LOCUS       DATCCH010000001         2224 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694 k141_109,
            whole genome shotgun sequence.
ACCESSION   DATCCH010000001 DATCCH010000000
VERSION     DATCCH010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="VIJ44_00005"
     CDS             complement(<1..373)
                     /locus_tag="VIJ44_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164307.1"
                     /translation="MATSVEDLPTKVTPFVLRAADSMCPRRLASELQSEPGTTDPVNR
                     ARVRNGLLDAVRTWHATGEWVVPPGLVAEERAVIAQAARWYEHHFPARAVEVTLPIES
                     PTPLSRRHLLLGGWVDLGVVHP"
     gene            442..2196
                     /locus_tag="VIJ44_00010"
     CDS             442..2196
                     /locus_tag="VIJ44_00010"
                     /inference="COORDINATES: protein motif:HMM:NF024594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HEY5164308.1"
                     /translation="MLPELDPIRRARLRTALRVALVVVLAWLLASAGLIFFARQQVSS
                     GLDTLQSAKNHLNADSLLRGQGLPALQQAERDFSSAHRLATNPVLAPWSVIPLAGGNV
                     DAIRALTGAAEHVAVVGTQAARDGSAALRSHPTTGAERLAVLDRVAAIAGRAARDLND
                     VSLGPDFFLVGPLGNARARFVDRLQRLRDAVTNAQALATGAAQVLRGPRRYLVLAANN
                     GEMRAGSGMFLSSGVATFGAGTFTLGEMGPSANRDLPASVPLPPQLAALWGWLAPGQH
                     WRNLATTPRFDVTAPVAAQMWQAATGEHVDGVLAVDAVALQALLAAQGPVVVGGQQLT
                     GSDALTYLLLGQYAGIPASAASQADRRDQLSAIAQASVETLSNRPWNAQTLVSELSGV
                     GKGRHILAWSRDSVEERAWEGAGIAGELHPDSLAVSIMNFGGNKLDQFLHVDSSLTVS
                     GRTDGGSDVRVDLRLHDDAPSGLPGYVSGPNPDSGAAEGVYQGVFAVNVPGAASLPLI
                     HGINPPLTAGVDGPTKAAAFGYFQVARGQTLDVTIRFRLPPSLRQITVEPSARVPPIA
                     WTFRSLKFTDTAAEHLGW"
BASE COUNT          315 a          836 c          748 g          325 t
ORIGIN      
        1 cgggatggac gacacccaga tcgacccacc cgccgagcag cagatggcgg cggctgagcg
       61 gggtcggcga ctcgatcgga agtgtcacct cgaccgcccg ggcgggaaag tgatgctcgt
      121 accagcgtgc ggcctgcgcg atcacggcgc gctcctcggc caccagcccc ggcgggacaa
      181 cccattcgcc ggttgcgtgc caagtccgga cggcgtcgag cagcccgttg cgaacccgcg
      241 cccggttcac cggatccgtc gtcccgggct cgctctgcag ttccgacgcc agtcgccgcg
      301 ggcacatcga atccgcggcg cgcaacacga acggcgtgac cttggtcggc aggtcctcga
      361 cgctcgtcgc cacgcgcgga ggctatggcg cagcgcgaca agagcgcggg atggagcggg
      421 cgcgctccta cacttccggt ggtgctgcct gagctcgacc cgataaggcg cgcgcgcctt
      481 cgtaccgcgc ttcgcgtcgc gctcgtcgtg gtgctcgcct ggctgctcgc cagcgctggg
      541 ctgatcttct tcgcgcgtca acaggtcagt tccggtcttg acacgctgca gagcgcgaag
      601 aaccacctga acgccgactc actcctccgc ggtcagggcc taccggcgct ccagcaggcc
      661 gaacgggact tctcgagcgc tcaccgcctc gccaccaacc cggtcctcgc cccctggtcg
      721 gtcatccccc tcgcgggggg caacgtcgac gcgatccggg cgctgaccgg cgcggccgag
      781 cacgtcgcgg tggtcggtac gcaggcggca cgcgacggat ccgcggccct tcgatcgcac
      841 ccgaccaccg gggccgagcg cctcgcggtc ctcgaccgag tcgccgcgat tgccggccgg
      901 gcggcacgcg acctcaacga tgtgagcctc ggccccgatt tcttcctggt cgggccgctc
      961 ggcaacgccc gagcccggtt cgtggatcgc ttacagaggc tccgcgacgc agtgacgaac
     1021 gcgcaagccc tcgcgaccgg cgcggcccag gtgctgcgcg gcccgcgccg ctacctcgtg
     1081 ctcgcggcca acaacggcga gatgcgcgcc gggtcgggca tgttcttgtc ctccggcgtg
     1141 gcgaccttcg gcgcggggac gttcaccttg ggagagatgg gccccagcgc caaccgggac
     1201 ctccccgcca gcgtgcccct tccgccccaa ctggcggcgc tgtggggctg gctcgcaccg
     1261 ggccagcact ggcggaacct ggcgaccacg ccccgcttcg acgtcaccgc cccggtcgcg
     1321 gcccagatgt ggcaggcggc caccggggag cacgtcgacg gcgtcctggc cgtggatgcc
     1381 gtcgcgctcc aagctcttct ggccgcccaa ggcccggtgg tggtcggggg tcaacagctc
     1441 accggaagcg acgcgctcac gtatctgctc ctcggccagt acgccgggat accggccagc
     1501 gcggcgtcgc aggccgatcg gcgcgaccag ctcagcgcca tcgcccaagc cagtgtcgaa
     1561 acgctctcga accgcccgtg gaacgcccaa acgttggtca gtgagctctc gggcgtcggc
     1621 aagggccgtc acatcctggc ctggtcgcga gattcggtcg aggagcgagc ctgggagggt
     1681 gccgggatcg ccggggagct ccaccccgac tcgctggccg tctcgatcat gaacttcggt
     1741 ggcaacaagc tcgaccagtt cctccacgtg gattcgtcac tcacggtgtc gggacgcacc
     1801 gatggtggaa gcgacgtgcg cgtcgatctc cggctccacg acgacgcccc gtcggggctg
     1861 ccgggctacg tgtcgggccc gaaccccgac agcggcgccg ccgagggggt ctaccagggg
     1921 gtcttcgcgg tcaacgtccc cggtgcggcg tcgctgccgc tcatccacgg gatcaacccg
     1981 cccctcaccg ccggggtcga cggaccgacg aaggccgccg ccttcgggta cttccaggtc
     2041 gcccgaggcc agaccctcga cgtgacgatc cggtttcggc ttccccccag tctccgccag
     2101 atcaccgtcg aaccgtcagc ccgggtcccg cccatcgcgt ggacgttccg gtccctcaag
     2161 ttcaccgata cggcggccga acacttgggg tggtgaccac cccactggcg ttccggtgac
     2221 gctc
//
LOCUS       DATCCH010000002         2395 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_248948, whole genome shotgun sequence.
ACCESSION   DATCCH010000002 DATCCH010000000
VERSION     DATCCH010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248948"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            388..1194
                     /locus_tag="VIJ44_00015"
     CDS             388..1194
                     /locus_tag="VIJ44_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164309.1"
                     /translation="MVLLALKLALTPLLIGGASLGARHWGPTAGGWIVSLPLTSGPVA
                     LFLALDRGPTFAADAAHASLAGCLAIAAYCLTYSRLAGRGWPAALAVATGGWLAGALA
                     AEATASWLVPATLALVVTATAGALAFLPARAAGRRAAPSGRGDMAIRMAVGAFVVVAV
                     TELAPFVGSAVAGLLAMLPIIGTILAVFAQRSGGPSDGVSVQRGILSGLFGTAAFLAV
                     VAWAIAPWGIAPAFLGAIATVAVVQVIALRVFTPTRGPASASHPAASQPA"
     gene            complement(1343..2203)
                     /locus_tag="VIJ44_00020"
     CDS             complement(1343..2203)
                     /locus_tag="VIJ44_00020"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5164310.1"
                     /translation="MSEREVRLSGTTVRPMLRDDVVAAADAVRRGDWGDREAFFRFAA
                     AHSNCRPILAERDGEIVGTGVATVSGRVGWVGTIWVAPALRGRGLGRAITEAVIDELE
                     GSGCRTLVLVATDAGRRVYERLGFEILTHDHALVAPGLGAGRPFSAPDPAIRAFARIQ
                     HLDAVAALDAWATGEDRRHILATCDGRVVVGAAGEVTAFALTSAFGGVATVARDVSDA
                     LRLFDLRRREAGPEDRIRAGAFDENVSGLERLVADGWTEVWSGSRMIRGQPLDWTPTA
                     IWSQFNHAIG"
BASE COUNT          316 a          880 c          832 g          367 t
ORIGIN      
        1 gcgtggtgac gatgcggcgt gaagggcggt cgggtcggcg tgccagtacg cggtccgcta
       61 tcggctcggc ctccgacggg cctcacgcag tctcccggta tcatcgcggg gtgaccgact
      121 tctacgacat cgaccgcgcg aacgctcgcc tccccgaggt ccggagccgg cacgcgcgag
      181 ctgccgacga ggacgcgacc tgcgtgtcat tggctcccgt caggcggcca tcggtatgcg
      241 cccggcggtc ccacgcgtca acggggtagt ccgacacgat gcccctgatc gtgctccgtc
      301 ggggagtgaa cctcgaggtt cggtgaggcg caccggacgc gctcggggca ccgcacggac
      361 gggtccgccg ccgctaccct tgacgatgtg gtcctcctgg cgctgaagct cgcgctgacg
      421 cccctcctca tcggcggggc gagcctcggc gctcggcact gggggcccac agcgggcggg
      481 tggatcgtct cgctgccgtt gacttcgggg ccggttgcgc tcttcctggc gctcgatcgg
      541 ggaccgacct tcgccgccga cgcggcccac gcctcgctcg ccggctgcct ggcgatcgcg
      601 gcctactgcc tcacctactc ccgtctcgcg ggccgcggct ggccagccgc cctggcggtc
      661 gcgaccggcg gctggctggc cggcgctctc gcggcggagg cgaccgcgtc gtggctcgtc
      721 cccgcgacgc tggcgctggt cgtgaccgcg acggccggcg cgctggcgtt cctgccggca
      781 cgcgcggcag gacgccgcgc ggcaccgtct ggccggggcg acatggcgat ccggatggcc
      841 gtcggggcat tcgtcgtggt cgcggtcacc gagctcgcac cgttcgtcgg ctcggctgtc
      901 gccggcttgc tcgccatgct gcccatcatc gggacgatcc tcgcggtgtt cgcgcagcgg
      961 tccggcggcc cgtcggacgg cgtcagcgtg caacgaggga tcctctcggg gctctttgga
     1021 acggccgcct tcctcgccgt cgtcgcctgg gccatcgcgc cctgggggat cgcccccgcg
     1081 ttcctcggcg ccattgctac ggtggcggtg gtgcaggtca ttgcgttgag ggtgttcacc
     1141 ccgacgaggg ggccggcgtc ggcgtcccat cccgctgcat cgcagccggc ttgaccaggg
     1201 cgaactggca cacccgaggg cgcgacaggt cgttaccgtg ccgatagcgg caggcgcacc
     1261 cgttcgcacg cggcacgggg tctggtcccc aaacgtcgcg catcgggcga atccgtcggg
     1321 acgaaacgac actgccgccc cttcaaccga tcgcgtggtt gaactggctc cagatcgcag
     1381 ttggcgtcca gtcgagcggc tgacctctga tcattcgcga gccgctccag acctccgtcc
     1441 agccgtcggc gacgagccgc tcgaggccac tgacgttctc gtcgaatgct ccggcgcgga
     1501 tgcggtcctc ggggccagcc tcccggcgcc gaaggtcgaa cagccgaagg gcatcggaaa
     1561 catcgcgcgc caccgtcgcg accccaccga acgcggatgt cagtgcgaat gccgtcacct
     1621 cgccagcggc tccaacgacg acccggccat cgcacgtcgc caggatgtgg cgtcggtcct
     1681 cgccggtcgc ccatgcgtcg agcgcggcga ctgcatcgag atgctggata cgggcgaagg
     1741 ctcgaatggc gggatccggc gcggagaagg gccgtccggc tccgagtcca ggcgcgacca
     1801 gggcgtgatc gtgggtgagg atctcgaagc cgagccgctc atagacccgg cggccggcgt
     1861 cggttgccac gagcacgagc gtccgacagc ccgagccttc gagctcgtcg atgaccgcct
     1921 ccgtaatcgc tcgcccgaga ccgcgccccc gcagtgccgg cgccacccat atcgtgccga
     1981 cccatccgac ccgccccgag acggttgcca cgcccgtccc aacgatctct ccgtcgcgtt
     2041 cggcaaggat cggtcggcag ttcgagtgcg cggcggcgaa gcgaaagaag gcctcccgat
     2101 cgccccagtc gcctcgtcga accgcgtcgg ccgccgcaac gacatcgtcc cgcagcatcg
     2161 gccggacggt ggtgccactg agccggactt cacgctcgct cacccgctga tggtggcagg
     2221 tccaccgttc gagcagtccc gacgacacgc cgtcacgatg gcaacgtcgt gttcggcgcc
     2281 aagacggcac gactcgagaa ctgcccgcgc cagcatggcc gctgtggccc ggctgccggt
     2341 ctactccagg tagtcccgca gcttgcgcga acggctcggg tggcgcagct tctgc
//
LOCUS       DATCCH010000003         2301 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_83105, whole genome shotgun sequence.
ACCESSION   DATCCH010000003 DATCCH010000000
VERSION     DATCCH010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83105"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="VIJ44_00025"
     CDS             complement(<1..271)
                     /locus_tag="VIJ44_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5164311.1"
                     /translation="MTLSGVAIVTGGAAGLGEAIAHRLGDEGAAVVVADLDGPGAERV
                     AAAITTAGGRAVGVVCDVTQPAQIAEAVHVATTLGPLRTLVLSAAV"
     gene            complement(268..1062)
                     /locus_tag="VIJ44_00030"
     CDS             complement(268..1062)
                     /locus_tag="VIJ44_00030"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEY5164312.1"
                     /translation="MTTTAANLDFRRLLDGQVALITGAASGQGRAAALLFGAHGAKVV
                     VADVNDEGSAETVRLLEEGDAEGVAIHADVSQRADVDTMVGAALDRFGHLDVLYNNAA
                     VQMSGRLVDCTDDEWDVTIATNLSAIFWACRAALPHLLEGRGGSIINTASTLGLIGSE
                     GYAAYGAAKAGLVLLTKQIAVEYGPAVRANVIAPGSIDTPRFRKVLEKTPGADQFVEG
                     LTRTIPARRLGMAEDVAAIALFLASDQSAYVSGAVVPADGGLAVYR"
     gene            complement(1136..1762)
                     /locus_tag="VIJ44_00035"
     CDS             complement(1136..1762)
                     /locus_tag="VIJ44_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013472229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEY5164313.1"
                     /translation="MPVDLPELLAPAHTAVLTMELQRGVVGDRATIAALADAVTTGDV
                     IGHAARVVQAARAVGARVVHCTAEFRPDRAGSADNAPLLRAIAKGPAHLLAGSLDTAL
                     VPELGPEPSDVIAARRHGLTPFPGTDLDQILRNLAVTTIVATGVSVNIGITGLVMVAV
                     DLGYRCAVVTDAVAGVPADYTAAVLDHTLAPLATLLSTEQVLATWSAA"
     gene            1928..>2301
                     /locus_tag="VIJ44_00040"
     CDS             1928..>2301
                     /locus_tag="VIJ44_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164314.1"
                     /translation="MAILVVVWLVCAFLGALIGSSKGQAAAGFFLGLLLGVIGLIIVV
                     FLKPADSSKQSKRVPCPHCAEQIMPAASVCPHCNREVNPRALVPASAGTAEGWMPDPS
                     GRHPDRWWDGRQQWTMWVRDRPG"
BASE COUNT          334 a          764 c          860 g          343 t
ORIGIN      
        1 cgacggctgc cgacagcacc aaggtccgca gcggaccgag ggtggtcgcg acgtgcaccg
       61 cctccgcgat ctgggccggt tgcgtcacgt cacacaccac cccgacggcc cggccgcccg
      121 cggtggtgat ggctgcggcg acccgctcgg ccccgggacc atcgagatcc gccaccacga
      181 ccgccgcgcc ttcgtccccg agccggtggg cgatggcctc accgagcccc gccgcgccgc
      241 cggtcacgat cgcgacccca gacagcgtca ccggtacacg gcgaggccgc cgtcggcggg
      301 gaccaccgcg ccactcacat aggcggactg gtcgctcgcc aagaacagcg cgatcgcggc
      361 gacgtcctcg gccatgccga gacgtcgcgc cgggatggtg cgggtgaggc cctcgacgaa
      421 ctggtcggcg cccggggtct tctcgagcac cttccggaac cgtggcgtgt cgatcgagcc
      481 gggcgcgatc acgttggcgc gcaccgccgg gccgtactcc accgcgatct gcttggtgag
      541 caggacgagc ccggccttcg ccgcgccgta ggcggcgtac ccctcggagc cgatcaggcc
      601 cagcgtcgac gcggtgttga tgatgctccc accgcggccc tcgaggaggt gagggagcgc
      661 ggcccggcag gcccagaaga tcgctgagag gttcgtcgcg atcgtgacgt cccattcgtc
      721 gtcggtgcaa tcgacgagac gaccgctcat ctgcactgcg gcgttgttgt agagcacgtc
      781 gaggtgaccg aaccggtcga gcgcggcgcc gaccatggtg tcgacgtcgg cgcgttggga
      841 cacgtcggcg tggatggcca cgccctccgc gtcaccttcc tcgaggagcc gaaccgtctc
      901 ggccgagcct tcgtcgttga cgtcggcgac gacgaccttg gcgccatggg ccccgaagag
      961 caacgcggcg gcgcgaccct gtccggacgc tgcaccggtg atgagcgcca cctggccgtc
     1021 gaggagacgt cggaaatcga ggttggcggc ggtggtcgtc atcgcggttc cgcattctgc
     1081 ctgggctttg atccggatgc ggtcggcggt gactcgggtc gacgatcggc gctgctcatg
     1141 ccgcggacca ggtcgcgagg acctgttcgg tgctgaggag cgtcgccagc ggagcgaggg
     1201 tgtggtcgag caccgcggcg gtgtagtcgg ccgggacgcc ggccacggcg tcggtgacga
     1261 cggcgcagcg gtagcccagg tcgacggcga ccatgacgag cccggtgatc ccgatgttca
     1321 ccgagacgcc ggtggcgacg atggtcgtca cggcgaggtt tcggaggatc tggtcgaggt
     1381 cggtgccggg gaacggggtg aggccgtgac ggcgggcggc gatgacgtcg gaaggctccg
     1441 gcccgagctc gggcaccagt gcggtgtcga ggctcccggc gaggagatgc gcgggaccct
     1501 tcgcgatggc gcgtagcagc ggcgcgttgt cggccgatcc ggcgcggtcg ggtcggaact
     1561 ccgcggtgca gtgcacgacc cgagccccga cggcccgggc cgcctggacc acgcgcgccg
     1621 catggccgat cacatccccg gtggtcacgg cgtcggccaa cgcggcgatg gttgcgcggt
     1681 caccgacgac gccccgctgg agctccatgg tgaggaccgc ggtgtgcgcg ggggcgagga
     1741 gctcagggag gtcgacgggc acgagacagg aacttaggcc cgggcggggc ggcggtgagg
     1801 cgaccgactc ggccgctcat cacctcccac cgcggcccgc cgaacggcac ggccgtgtcg
     1861 tccgattcct ggccggagga tggtgtccac gacagaatga cctgtcaccg ggtggaaggg
     1921 ggcagccatg gccatcctgg ttgtggtttg gctcgtgtgc gcttttctcg gcgcgcttat
     1981 cggttcgtcg aaaggacagg cggcggccgg gttcttctta ggtctgctgc tgggcgtcat
     2041 cggcctcatc atcgtggtgt tcttgaaacc ggccgattcg tccaagcagt ccaagcgggt
     2101 cccgtgtccc cactgcgctg aacagatcat gcccgcggca tcggtctgtc cgcactgcaa
     2161 ccgagaggtg aatccgaggg cgctggttcc ggcatcggcg ggaaccgccg agggatggat
     2221 gcccgacccc agtggacgac accctgaccg ttggtgggac ggcagacaac agtggacgat
     2281 gtgggtgcgt gaccggcctg g
//
LOCUS       DATCCH010000004         4455 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_497803, whole genome shotgun sequence.
ACCESSION   DATCCH010000004 DATCCH010000000
VERSION     DATCCH010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4455
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497803"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /gene="rplB"
                     /locus_tag="VIJ44_00045"
     CDS             <1..755
                     /gene="rplB"
                     /locus_tag="VIJ44_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002528761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEY5164315.1"
                     /translation="PEKSLLAPKPSTGGRNSYGRKTSRHRGGGHKQQYRIIDFKRDKD
                     GVPAKVATIEYDPNRNARIALLHYLDGEKRYIIAPALVAVGDLLQSGHGSEIRPGNAL
                     PMRYIPVGTTIHNVELKPGAGAKFGRGAGAAIQLVAKEGQFATLRLPSTEMRRVPIDC
                     RATVGAVGNAEATLVKIGKAGRNRWRGKRPHTRGVAMNPVDHPLGGGEGKSSGGRHPV
                     SPWGKPEGRTRKKGKKSDQMIVRRRRSRGARR"
     gene            774..1055
                     /gene="rpsS"
                     /locus_tag="VIJ44_00050"
     CDS             774..1055
                     /gene="rpsS"
                     /locus_tag="VIJ44_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008339784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEY5164316.1"
                     /translation="MPRSLKKGPFVDEHLAKKVDAMNAANDRKVIKTWSRRSTVTPEM
                     VGHTIAVHDGHKHVPVYISESMVGHKLGEFAPTRTFRFHSGQERGVRGR"
     gene            1062..1742
                     /gene="rplV"
                     /locus_tag="VIJ44_00055"
     CDS             1062..1742
                     /gene="rplV"
                     /locus_tag="VIJ44_00055"
                     /inference="COORDINATES: protein motif:HMM:NF012462.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEY5164317.1"
                     /translation="MPDTATRETSARLRHARISPPKVRMVLELIRGQDVETARDTLRF
                     CERSAAREVGKLLDSAIANAEHNNHIPEDELFVTRAHADEGPTLKRWRPRARGRGVPV
                     RKRTSHVTIVLARFADDEMQRRRGVDAQTGRGRRRADRRRPAELPAAEPDVEVTPDAD
                     EETPVGETAPEPAPAKTTKKASTKKATTKKATTKKATTKKATGASAKKTTPRKAPAKR
                     PGQPKDKD"
     gene            1742..2677
                     /gene="rpsC"
                     /locus_tag="VIJ44_00060"
     CDS             1742..2677
                     /gene="rpsC"
                     /locus_tag="VIJ44_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEY5164318.1"
                     /translation="MGQKVNPYGFRLGITTDWKSRWFADGKEYTDSLLEDWQIRDYLR
                     RQLPHAAVSRVEIERTRDRLRVDVYTARPGIVIGRRGAEADRLRQGLYKITGNPKVQF
                     NIQEIKQPELDATLIAQGVADQLAGRVSFRRAMKRAVQTAMKAGAQGIRVQCGGRLGG
                     AEMSRKEWYREGRVPLHTLRADIDYGGAEARTTFGRIGVKVWIYKGEILPYKSAAEDK
                     ITREAAMAVGETAGGEGTPRRRVVSAGGGRRRREETVEVPVEAGTEVVPEEATPLVKE
                     ADPELERLLAEEEEIERRTRQGQHHEAPKFHSDDE"
     gene            2700..3116
                     /gene="rplP"
                     /locus_tag="VIJ44_00065"
     CDS             2700..3116
                     /gene="rplP"
                     /locus_tag="VIJ44_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEY5164319.1"
                     /translation="MLMPRKIRHRKVQRGRRTGVAKGGTRVHFGDYGIQALEAGWITN
                     RQIEAARIAMTRYIKRGGKVWINIFPDKPVTQKPAETRMGSGKGNPEHWVAVVKPGRV
                     MFELAGVPESTARDAMRLAMHKLPIKTRFVVRQEEA"
     gene            3116..3334
                     /gene="rpmC"
                     /locus_tag="VIJ44_00070"
     CDS             3116..3334
                     /gene="rpmC"
                     /locus_tag="VIJ44_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003247579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEY5164320.1"
                     /translation="MKAVELRELNDIELANRVREFREELFNLRFQNATGQLENSTRLN
                     HVKKDIARCETLLREREIAAAEALKEHA"
     gene            3331..3606
                     /gene="rpsQ"
                     /locus_tag="VIJ44_00075"
     CDS             3331..3606
                     /gene="rpsQ"
                     /locus_tag="VIJ44_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014909496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEY5164321.1"
                     /translation="MSEPDASTRPNPRKTREGLVLSSKMDKTVVVAVTDRVRHRRYGK
                     TLQRTRRLYAHDEANDAHEGDRVRIAETRPLSRLKHWRVIEVLERAR"
     gene            3603..3971
                     /gene="rplN"
                     /locus_tag="VIJ44_00080"
     CDS             3603..3971
                     /gene="rplN"
                     /locus_tag="VIJ44_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEY5164322.1"
                     /translation="MIQQESRLKVADNSGAREVLCIKVLGGTRRRYAGIGDIFVATVK
                     DAVPGAAVKKGEVVKCVVVRTKKERRRPDGSYIRFDENAAVLINEQMQPRGTRIFGPV
                     GRELRDRRFMRIVSLAPEVL"
     gene            3991..4296
                     /gene="rplX"
                     /locus_tag="VIJ44_00085"
     CDS             3991..4296
                     /gene="rplX"
                     /locus_tag="VIJ44_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEY5164323.1"
                     /translation="MRLRKGDRVRVLSGKDLGKEGEITRVLPERNMVIVDGVNIAKKH
                     QRSTSATMQGGIIDKDMPVPASNVAILCPSCGPSRIGYQFDGDGVKTRVCKKCGKGL"
BASE COUNT          892 a         1552 c         1451 g          560 t
ORIGIN      
        1 gccctgagaa gtcgctgctg gcccccaagc cgtcgaccgg cggtcgcaac agctacggtc
       61 gcaagacgtc gcgtcaccgt ggcggcggtc acaagcagca gtaccggatc atcgacttca
      121 agcgggacaa ggacggcgtg ccggccaagg tggccaccat cgagtacgac cccaaccgca
      181 acgcccgcat cgcgctgctc cactacctcg acggcgagaa gcgttacatc atcgcccccg
      241 cgctggtggc ggtcggcgac ctcctccaga gtggtcacgg ctccgagatc cggcccggga
      301 acgccctgcc gatgcgatac atcccagtcg ggaccacgat ccacaacgtc gagctcaagc
      361 ccggagcagg cgccaagttc ggccggggcg ccggcgccgc catccagctc gtcgccaaag
      421 agggccaatt cgccacgctg cgcctcccgt ccaccgagat gcgtcgcgtc ccgatcgact
      481 gccgagccac cgtcggggcg gtcggcaacg ccgaagccac cctcgtcaag atcggcaagg
      541 ccggccgaaa ccgttggcgg gggaagcggc cccacacccg cggcgtcgcg atgaaccccg
      601 tcgaccatcc cttgggcggt ggggaaggca agagctcggg aggccgtcat ccggtctccc
      661 cgtggggcaa gcccgagggg cgcacccgca agaagggcaa gaagtccgac cagatgatcg
      721 tgcgccgacg ccgcagccgc ggcgcgcgcc gataggggag cgagagggac accgtgccgc
      781 gcagcctcaa gaaaggaccg ttcgtcgacg agcacctcgc caagaaggtg gacgcgatga
      841 acgctgccaa cgaccgcaag gtcatcaaga cgtggtcccg acggtcgacg gtcacccccg
      901 agatggtcgg tcacaccatc gcggttcacg acgggcataa gcacgtcccc gtttacatca
      961 gcgaatcgat ggtcgggcac aagctcggcg agttcgctcc tacccgcacc ttccggttcc
     1021 actccggtca ggagcgaggc gtgaggggtc ggtagctcga gatgcccgac accgcgaccc
     1081 gagagacgag cgcacggctc cgccatgcgc gaatctcgcc acccaaagtc cgtatggtcc
     1141 tggagctgat ccgcgggcaa gacgtcgaga ccgcgcgcga caccctccgg ttctgcgagc
     1201 ggtccgcggc ccgcgaggtc ggcaagctgc tcgattcggc cattgccaac gccgaacaca
     1261 acaaccacat ccccgaagac gagttgttcg tgacccgcgc gcacgccgat gaggggccca
     1321 ccctcaagcg gtggcgcccc cgagcgcgcg ggcgtggggt gcctgtccgc aagcggacca
     1381 gtcacgtgac catcgtgctc gcccggttcg ccgacgacga gatgcagcgg cggcgcggtg
     1441 tcgatgcgca gaccggccgc gggcgtcgtc gcgctgaccg ccgtcgtccc gctgagctcc
     1501 ccgcggccga gcccgacgtc gaggtcaccc ccgacgccga cgaggagacc cccgtcggcg
     1561 agacagcccc ggaaccggcg ccggccaaga ccaccaagaa ggcgtcgacg aagaaggcca
     1621 cgacgaagaa ggcgacgacc aagaaggcga cgaccaagaa ggcgaccggc gcctccgcca
     1681 agaagacgac gccccgcaaa gcaccggcga agcgaccggg ccaaccgaag gacaaggact
     1741 gatgggccaa aaggtcaacc cgtacgggtt ccggctcggg atcacaaccg attggaaatc
     1801 gcggtggttc gccgacggca aggaatacac cgacagcctc ctcgaggact ggcagatccg
     1861 cgactacctc cgccgccagc tccctcacgc ggccgtgagc cgggtcgaga tcgaacgcac
     1921 ccgcgaccgg ctgcgagtcg acgtctacac cgcccgcccg gggatcgtca tcggccgccg
     1981 cggcgccgaa gcggaccgcc tccgccaagg cctctacaag atcaccggca accccaaggt
     2041 ccagttcaac atccaagaaa tcaagcagcc cgagctcgac gcgaccctga tcgcccaggg
     2101 cgtcgctgac cagctcgccg gtcgagtctc gttccgccgg gcgatgaaac gcgctgtcca
     2161 gactgccatg aaggccggcg cccagggcat ccgcgtgcaa tgcggcggcc gcctgggcgg
     2221 cgccgagatg tcccgtaagg agtggtaccg ggaagggcgg gtacccctcc acacgctgcg
     2281 ggccgatatc gactacggcg gcgctgaagc gcgaaccacg ttcgggcgca tcggcgtcaa
     2341 ggtctggatc tacaagggtg agatcctgcc ctacaagtcc gcggccgagg acaagatcac
     2401 ccgcgaggcc gccatggcgg tcggcgagac cgccggcggc gagggcacgc cccgtcgacg
     2461 cgtggtgagc gccggtggcg gtcgccgtcg ccgcgaggag accgtcgaag ttccggttga
     2521 ggcggggacc gaggtcgtcc ccgaggaggc gacaccgctc gtcaaagagg ccgaccccga
     2581 actcgagcgg ttgctcgccg aggaagaaga gatcgagcgt cggacccgcc agggccagca
     2641 ccacgaagca cccaagttcc actccgacga cgaatgagcg acacggacaa ggcctgacca
     2701 tgttgatgcc ccgcaagatc cgccaccgca aggtgcagcg cggccgccga accggcgtcg
     2761 cgaagggtgg cacgcgcgtg catttcggcg actacggcat ccaggcgctc gaagccgggt
     2821 ggatcaccaa ccgccagatc gaagcggccc ggatcgccat gacccgctac atcaagcggg
     2881 gcgggaaagt ctggatcaac atcttccccg acaagccggt cacccagaag ccggccgaga
     2941 cgaggatggg ctccgggaag ggcaatcccg agcactgggt ggcggtcgtg aagcccggtc
     3001 gggtcatgtt cgagctcgcc ggagtaccgg agagcaccgc ccgggatgcc atgcgcctgg
     3061 cgatgcacaa gcttccgatc aagacacgct tcgtcgtccg tcaagaggag gcgtgatgaa
     3121 ggccgtcgag ctccgggagc tcaacgacat cgagctcgcc aaccgcgtcc gcgagttccg
     3181 agaggagctg ttcaacctgc ggttccaaaa cgccaccggt cagctcgaga actccacccg
     3241 gttgaaccat gtgaagaagg acattgcgcg ctgcgagacc ctgctccgcg agcgcgagat
     3301 cgccgccgcc gaggccctga aggagcacgc atgagcgagc ccgacgcttc cacgcgtccc
     3361 aacccgcgaa agacccgtga agggctcgtg ctgtcctcga agatggacaa gacggtcgtc
     3421 gtcgccgtca ccgaccgtgt ccgtcaccgc cgctacggca agacgctgca acgcacccgt
     3481 cggctctacg cccacgacga ggccaacgat gcccacgaag gcgaccgcgt ccgcatcgcc
     3541 gagacccgtc cgctgtcgcg actcaagcac tggcgcgtca tcgaggtcct ggagcgcgcc
     3601 aggtgatcca acaggaatct cgtctcaagg tcgccgacaa ctcgggtgcg cgcgaggtct
     3661 tgtgcatcaa ggttctcggt ggaaccaggc gtcgctacgc ggggatcggc gacatcttcg
     3721 tcgcaaccgt caaggacgcc gtccccggtg ctgcggtcaa gaagggtgag gtcgtcaagt
     3781 gcgtcgtcgt tcgcaccaag aaggaacgcc gacgccccga cggcagctat atccgcttcg
     3841 acgagaatgc ggccgtgctc atcaacgagc aaatgcaacc tcgcgggacc cgaatcttcg
     3901 gccccgtcgg ccgggagctt cgtgaccgcc ggttcatgcg catcgtctca ctggccccgg
     3961 aggtgctctg acatggctct tcgcgcccgg atgcggctcc gcaaagggga ccgggtacgg
     4021 gtcctctccg gaaaagacct cggcaaagaa ggcgagatca cccgagtgct gcccgagcgc
     4081 aacatggtga tcgtcgacgg cgtcaacatc gccaagaagc accaacgctc caccagcgcc
     4141 accatgcaag gcggcatcat cgacaaggac atgccggtac cggcgtcgaa cgtggcgatc
     4201 ctctgcccga gctgcggtcc gagtcgaatc ggttaccagt tcgacggcga cggggtcaag
     4261 acgcgggtgt gcaagaaatg cgggaaaggc ctctgatggc gaccgtacgc gagaagcccc
     4321 gcctcaaggc ccgctacgac gacgcgctgc gcccccagct ccaacaggaa ctcggtctgc
     4381 gcaacgtcat ggaagtgccc cgcctcgaca agatcgtcct caactgcggg gtgggtcaag
     4441 cgacccagca ggcct
//
LOCUS       DATCCH010000005         2513 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_249068, whole genome shotgun sequence.
ACCESSION   DATCCH010000005 DATCCH010000000
VERSION     DATCCH010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249068"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1675)
                     /locus_tag="VIJ44_00090"
     CDS             complement(<1..1675)
                     /locus_tag="VIJ44_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164324.1"
                     /translation="MSRVPTVERLGSPAAREKLPPVAPIATGAAREARSGAVAATSPV
                     RDRTLVAVLAVAVGLTAFGLARHAMWFDELQAWNLARASRSVPDLLHHLRYEGHPPAW
                     YLVLYGLTRITGDPRAMQGAELVIVAATYAVVLFASPFSKPVRFALLAGYTISFEYGV
                     ISRGYGLGVLSLVVTLALLGRPRPRWRWALVSAAVLAWTSVAGAVVALAIAGAVAGGA
                     PHRGGRTPGPGRDRRRFVVGTGVAAVAAAVVCIPPSDFHAFTPSVGSVSTLGTAGWSR
                     LLTAATGTWRGLVPIPSRLGAWNSQFVDRLPAAAWIEAGLSIAVVALMAGALRRFRTA
                     WRLWLLGSVGSFLFFAVVVLPDQARYAGTTFLVFLGAAWWAYAPPGGADDGSTGAVGP
                     GSARPSRRLHQLVTVVIAAQVVALLAIYPSETSGRFAPNQAVAEVVRAAGLQDHVVSG
                     QDFDATSVAAYLDRPVYSVARQAWIRYFTHDDREARRYNALGAAEILCAAWRVARGAH
                     HAAAIITEHLTPHRDPGVARIVTIDQVEVIRVDPDATLAGCADPASRRDA"
     gene            1932..2123
                     /locus_tag="VIJ44_00095"
     CDS             1932..2123
                     /locus_tag="VIJ44_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017832546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEY5164325.1"
                     /translation="MNLFVIQSWLQAKLGRDERGANLVEYILLVALIALAVIAAVVFL
                     RGQVNSQFNNAGSHLSSGS"
     gene            2283..>2513
                     /locus_tag="VIJ44_00100"
     CDS             2283..>2513
                     /locus_tag="VIJ44_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164326.1"
                     /translation="MSTVAVDPRAARRLGVRVELAPREWWALRWISEQDAVRRDVVAH
                     VLGGPEPLASDQTRRVVDRWRRAGLIERERLLA"
BASE COUNT          390 a          900 c          912 g          311 t
ORIGIN      
        1 tcgcgtcgcg gcgcgacgcg gggtcggcgc agcccgccag ggtggcgtcg ggatccacgc
       61 ggatgacctc gacctggtcg atggtgacga ttcgcgccac gccgggatcc ctatgcggcg
      121 tcaggtgctc ggtgatgatc gccgcggcgt gatgggcacc tcgggcgacg cgccaggcgg
      181 cgcacaggat ctccgcggcc cccagcgcgt tgtatcggcg cgcctcgcgg tcgtcgtggg
      241 tgaagtagcg gatccacgct tggcgagcga ccgagtagac gggacggtcg aggtaggcgg
      301 cgacgctggt ggcgtcgaag tcttgtccgg acacgacgtg gtcctggagt cccgcggcgc
      361 gcacgacctc ggccacggcc tggttcggtg cgaagcggcc cgaggtttcc gacgggtaga
      421 tggcgagcag cgcgacgact tgggccgcga tcacaaccgt gacgagctgg tgcagccgcc
      481 gcgatggccg ggctgacccg ggaccgaccg cccccgtcga cccgtcgtcg gctccgcccg
      541 ggggggcgta cgcccaccac gccgcgccca agaacacgag gaacgtcgtg cccgcgtacc
      601 gggcctggtc gggcaggacc acgaccgcga agaagaggaa cgagcccacc gacccgagca
      661 gccacagacg ccaggcggtg cggaagcgac gcagcgcgcc ggccatgagc gccacgaccg
      721 cgatcgagag cccggcctcg atccatgccg cggccgggag gcgatcgacg aactgcgaat
      781 tccatgcccc cagccgggac gggatcggca ccaggccccg ccaggtcccg gtcgcggctg
      841 tgagcagccg cgaccaaccc gcggtgccga gcgtcgagac gctgcccacg ctgggcgtga
      901 acgcatggaa gtcggacggc gggatgcaca cgacggcggc ggcgaccgcc gccacgcccg
      961 tccccacgac gaagcggcgc cggtcccggc ccggtcccgg tgtccgccca cccctgtggg
     1021 gggcaccgcc ggcgaccgcg cccgcgatgg ccagggccac gaccgcgcca gcgacactgg
     1081 tccacgcgag caccgcggcg ctgaccagcg cccagcgcca ccggggtcga ggccggccga
     1141 gaagcgcgag ggtcacgacg aggctgagga caccgagccc gtagccccga ctgatgacgc
     1201 cgtactcgaa gctgatggtg tagccggcca gcagcgcgaa ccgcaccggc ttggagaacg
     1261 gggacgcgaa gagcacgacc gcgtatgtgg ccgccacgat caccagctcg gcgccctgca
     1321 tggcacgcgg gtcaccggtg atccgcgtca ggccgtagag cacgaggtac caggcaggcg
     1381 gatggccctc gtagcggagg tggtgcagca ggtccgggac cgaacggctg gcccgggcga
     1441 ggttccaggc ctggagctca tcgaaccaca tcgcgtgccg cgccaacccg aacgcggtga
     1501 gcccgaccgc gaccgcgagc accgccacca gggtccggtc ccgaaccggg ctggtggcgg
     1561 cgaccgcccc ggaccgggct tcccgggccg caccggtggc gatcggcgcc accggcggga
     1621 gcttctccct cgccgccggc gacccgagcc gctcgacggt cgggacccga ctcatcgcct
     1681 ggtccgatgc gcctccggtc gacccgggcg accgggtcgt cggggcgccg ggatcgggct
     1741 cggaaacgcc ggcaccgggg gagaggccct cacggagcgg tgatcggccc cgccgcttcc
     1801 ggactgaagc cggggccgag gtcgcgctca agatccaggg agacgctgcc gatatccgtc
     1861 cccggacggc gggcttccgg gaggtcgtgg gctacgactc ggaaaccaaa acggaaggac
     1921 ggggctacac aatgaacctt ttcgtcattc agagctggct gcaggccaag ctcggtcggg
     1981 atgagcgggg cgccaacctg gtcgagtaca tcctgttggt cgcgctcatc gcgcttgcag
     2041 tcatcgctgc ggtggtcttc ctccgcggtc aggtgaacag ccagttcaac aacgctggca
     2101 gccacctgtc gagcggctcg taagcacgac tacctcgcag ggacggccgg tcccggtctc
     2161 accaccggag cggtcgtcac ctccagcact cgatcaaggc cgtcccttcg aggacggcct
     2221 tgatcacgtt gtctccccgt tcccgatcac cgccggcgcc cgaccgggtc ttggtgggtg
     2281 tgatgtccac cgtcgccgtc gatccacgcg cggcgcgacg tctcggcgtc cgggtcgaac
     2341 tcgcaccccg cgaatggtgg gcgctgcgat ggatctcgga gcaagacgcc gtgcgccgcg
     2401 acgtcgtcgc ccatgtgctc ggcggccctg agccgctcgc ctcggaccag acgcgacgcg
     2461 tcgtcgaccg ctggcgtcga gccggtctga tcgaacggga gcggcttctc gcc
//
LOCUS       DATCCH010000006         2927 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_332123, whole genome shotgun sequence.
ACCESSION   DATCCH010000006 DATCCH010000000
VERSION     DATCCH010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332123"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="VIJ44_00105"
     CDS             complement(<1..607)
                     /locus_tag="VIJ44_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012946341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEY5164327.1"
                     /translation="MTPTDGSSAQLLPRLLVLTDAAMLPDGRDLTSTLRAAVEGGARA
                     VVLRERDLPTPVRARLADQVQALLSPVGGVLLLASAAPPEATSRPAGLHLRSADPIPA
                     TRPALLGRSTHSADEVLHAAAEGCDYVTLSPVAPTRSKPGYGPSLGAAGFGSVLAQAR
                     RVRRPLPAVFALGGVDEHNAASFIQAGAHGVAVMGSVMSSPD"
     gene            complement(604..1419)
                     /locus_tag="VIJ44_00110"
     CDS             complement(604..1419)
                     /locus_tag="VIJ44_00110"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006606125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEY5164328.1"
                     /translation="MTVTDMSSVAPVVSSVPDPFVIAGEELSSRLLLGTGGATSLTTL
                     DSAVRASGSALVTVAIRRVSLTAGDSVLAVLNRAGVRLLPNTAGCYTAREAVLTAKLA
                     REALGTTWVKLEVIADDKTLLPDAVELLDAAERLVDDGFTVLPYTNDDPVLARRLAGL
                     GCAAIMPLGSPIGSGLGIRNPHAIEMICDAVDVPVVLDAGIGTASDAALAMELGCDAV
                     LIASAVTRAENPAAMATAMKHAVLAGRLARTAGRIPVRRLARASSPTEGVVVG"
     gene            complement(1421..1621)
                     /gene="thiS"
                     /locus_tag="VIJ44_00115"
     CDS             complement(1421..1621)
                     /gene="thiS"
                     /locus_tag="VIJ44_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018545575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEY5164329.1"
                     /translation="MTITLNGSPHEIADGTTLDQLVTVFSLPERGVALAMNSEVVPRS
                     VWASTRVLLDARVEVVTAFQGG"
     gene            complement(1618..2841)
                     /gene="thiO"
                     /locus_tag="VIJ44_00120"
     CDS             complement(1618..2841)
                     /gene="thiO"
                     /locus_tag="VIJ44_00120"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018104428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HEY5164330.1"
                     /translation="MTDAQRARTKVHTPGERSDVRVAVIGGGVIGLSIAWTLARDGCD
                     VEVHDDQPGLGASYAAAGMLAPVCEAAYEEPELLALGLASVQAWPEFAARLTQASGVD
                     VGLREEGSLWVGFDTDDAIGLGRVADLLARHDLRHRRLSSREARALEPALSPRISSAL
                     QVPGDHSVDNRQVVRALLAAAERAGVRIHRQRVGLVTVDGRAVGVRRVDVGDLNSAMA
                     PVHEADLVVLAAGDGSAHVPGLPDCARYPVRPVKGQILRLAGATGLLERTVRATVHGE
                     KVYLVPRGNGEVVVGATMEEVGADLTVTAGAVHHLLRTAIEVVPEVAELELVEALARC
                     RPGTPDNGPLIGYTPLPGLLVATGHGRGGVLLAPTTSDALHRLVSDLPMSVVIEPFAP
                     QRFSRSAHDVTEGIA"
BASE COUNT          460 a         1012 c         1047 g          408 t
ORIGIN      
        1 ggtcgggtga gctcatgacc gaacccatca cggccacgcc gtgtgcgccg gcctgaatga
       61 acgatgcggc gttgtgttcg tcgacaccac cgagggcgaa gacggccggc agcggcctgc
      121 ggacacgacg agcctgggcc agcacgctgc cgaaacctgc cgcgccgagc gacggcccgt
      181 acccgggctt ggatcgggtc ggggcgaccg gcgacagggt gacgtagtcg cagccttcgg
      241 cggctgcgtg gagcacctcg tcggccgaat gtgtcgaacg ccccagcaag gcaggcctcg
      301 tagccgggat cgggtcggcg ctgcgcaggt gtagcccggc cggtcgggag gtcgcctctg
      361 gtggtgcagc tgatgcgagg agcaggacgc cgcccacggg tgagagcagc gcctgcacct
      421 ggtcggcgag gcgggcccgg acgggggtgg gcaggtcgcg ttctcgcagc acgacggccc
      481 gggcgccgcc ctcgactgca gcacgcaggg tgctggtcag gtctcggccg tcaggcagca
      541 tcgcggcgtc ggtcagcacg agaagccgtg gcagcagctg ggccgagctg ccgtcggtcg
      601 gggtcatccc acgaccacac cctcggtcgg tgaggatgcg cgggcgagcc ggcgcaccgg
      661 gatacggccg gctgtgcggg cgaggcgacc ggcgagcacg gcgtgcttca ttgcggtggc
      721 catcgcggcg gggttctcgg cgcgggtgac cgcgctggcg atgaggacgg cgtcacaacc
      781 cagttccatg gccagcgcgg cgtcggacgc ggtgccgatc ccggcgtcca ggaccacagg
      841 cacgtcgacg gcgtcgcaga tcatctcgat tgcgtggggg ttacggatgc ccaggccgct
      901 gccgataggt gagcccagcg gcataatcgc cgcgcagccg aggccggcca gccggcgggc
      961 gagcaccggg tcgtcattcg tgtagggcag gaccgtgaac ccgtcatcca cgagacgctc
     1021 tgccgcatcc aacagctcga ccgcgtcggg caggagcgtc ttatcgtcgg cgatgacctc
     1081 gagcttgacc caggtcgttc cgagcgcctc tctggccagc ttggccgtga gcaccgcctc
     1141 ccgtgctgtg taacagccgg cggtattggg cagcagccgc accccggcgc ggttcaggac
     1201 ggccaggacg ctgtcgcccg cggtcagcga gacccgtcgg atcgcgaccg tgaccagcgc
     1261 cgaccccgac gcgcgcacgg cgctgtccag cgtcgtcagg ctggtcgccc caccggtgcc
     1321 cagcaagagc cgcgaggaca gctcctcccc ggcgatgacg aacgggtccg gcaccgaact
     1381 cactaccggt gcaacggaac tcatgtcggt gacggtcatc tcagccaccc tgaaaggcgg
     1441 tgacgacctc gacccgcgca tcgagaagga cccgcgtgct cgcccacacc gaccgaggga
     1501 cgacctcgct gttcatcgcc agcgcgaccc cccgctcggg taatgagaag accgtgacga
     1561 gctggtcgag tgtggtcccg tccgcgatct cgtgggggga gccgttgagc gtgatggtca
     1621 tgcgatgccc tccgtgacgt cgtgagctga gcgtgagaaa cgttgtggcg caaaaggttc
     1681 gatgaccaca gacatcggga gatccgagac gaggcgatgg agggcgtcgc tcgtcgttgg
     1741 agcgagcagc acgccgccgc ggccatgccc tgttgccaca agcagacccg gcagcggcgt
     1801 gtaaccgatc agcggcccgt tgtcgggggt gccgggtcgg caccgggcga gcgcctcgac
     1861 cagctcgagc tcggcgacct cgggcacgac ctcgatcgcg gtgcgcaaca aatggtgaac
     1921 ggcaccggcc gtcacggtga ggtcggcccc gacctcttcc atggttgctc ccacaaccac
     1981 ctcaccgttg ccgcggggaa cgaggtagac cttctccccg tgaaccgtcg cgcggaccgt
     2041 gcgctccagc agcccggtag ctcctgccag ccgcaggatc tggcccttaa ccgggcggac
     2101 cgggtaacgc gcgcaatccg ggagcccggg aacgtgagcc gagccgtcgc ccgcggccag
     2161 gacgacgagg tcggcctcgt ggaccggcgc catcgcagag ttcagatcgc cgacatcgac
     2221 gcggcggacc ccgaccgctc gcccgtcgac ggtgaccaga ccgacacgct ggcgatggat
     2281 ccgcacaccg gcgcgctcgg ctgcagcgag cagtgctcgc acgacctggc ggttgtcgac
     2341 gctatggtca cccgggactt gcagcgccga acttattcga ggcgagagcg ccggctccag
     2401 cgcgcgagcc tcacgggaac tcagtctgcg gtgccgcagg tcgtgacggg cgagcaggtc
     2461 ggcgacccgc cccaggccga tcgcgtcgtc cgtgtcgaag ccgacccaca gcgagccctc
     2521 ctcgcgcaac ccgacatcga cgcccgacgc ctgggtcagt cgagccgcga actcgggcca
     2581 tgcctgcacc gaagccaggc cgagggccaa gagctccggt tcctcatacg ccgcctcgca
     2641 caccggcgcg agcatgcccg cggcggcata tgaagccccc aaacccggct ggtcgtcgtg
     2701 gacctcgacg tcgcatccgt cccgcgccag ggtccacgcg atgctgagcc cgatcacacc
     2761 gccgccgatg acggcgaccc ggacatcgga ccgctcgccg ggcgtgtgga ccttggtccg
     2821 ggctcgctgg gcatcggtca tgaggttctc ctggttctgg gtgctgggtg gcgggttggt
     2881 tggtggtaca cgtgcctgac gggtcctcgg cttcggcctc ggcgatg
//
LOCUS       DATCCH010000007         3620 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_166636, whole genome shotgun sequence.
ACCESSION   DATCCH010000007 DATCCH010000000
VERSION     DATCCH010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3620
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166636"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="VIJ44_00125"
     CDS             complement(<1..521)
                     /locus_tag="VIJ44_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014374457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HEY5164331.1"
                     /translation="MSLAVLDWRRRVTELYTDVRATDDPATGHIIWRHGRDHLFADHP
                     ASPLLAADRAAFTGLRYAPYDPTLRFTLDIDTASPAAALEVPTGTDGIVTFRRVGQAR
                     LPGFGNLDVWWLLGYGGGLFLPVKDPNPETYGGGRYLLDTAKGADLGGHVDPNGTTTL
                     LIDLNFAYNPSCA"
     gene            complement(572..1108)
                     /locus_tag="VIJ44_00130"
     CDS             complement(572..1108)
                     /locus_tag="VIJ44_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164332.1"
                     /translation="MHWIGAVVVAAALGATAASTRSPRVSGGALGSYVHAVEPIRLGV
                     NDLLDGADPILESYREHQSTGDQAAAAMGTLERRFASYTVQINALTPANPTLAGLHAP
                     YAHTYILEDAYLGALTAALPSGDFSTLPNTQAAQRAAIIEWRTRLEVISRQMHTRLPS
                     DLQQAGRGEIAPAVSGAS"
     gene            1310..2260
                     /locus_tag="VIJ44_00135"
     CDS             1310..2260
                     /locus_tag="VIJ44_00135"
                     /inference="COORDINATES: protein motif:HMM:TIGR03621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03621 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEY5164333.1"
                     /translation="MPATGPIRFGIQVVAADSRSSLVELARRAESLGFDTLLVPDHVG
                     LPDPFVTLQAAADATTTLRVGTYVLNNDFHDPVLLARAAATLDVLSDGRFELGLGAGH
                     AQPEYEARGVAFEPAKQRVARLAEAVPLLRRLLDGESVTSEGVYYRLHNAACAPSPRQ
                     ARVPIVVGGNGAEVLRLAAREADGAGLTGLGRTLADGDRHEAEWSTASLEGRLQMIGD
                     AAGSRGQPELSALVQVVAVTDDRGDAARRVAERVPGLTETDALETPFLLLGSLPQMVE
                     QIVAAHQRWGFHYFVTRADSVNVMGDVIAEVHARSDPERP"
     gene            complement(2634..3023)
                     /locus_tag="VIJ44_00140"
     CDS             complement(2634..3023)
                     /locus_tag="VIJ44_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164334.1"
                     /translation="MKLGRWSSAALMASGLAGVVMPSRVASALELPAVTARGITETRA
                     GLGGTYAALGGWALVSRDPTAQAAVGVVWLGAAGARVGSLVLDQPRTDGAFWAYLTAE
                     ISLGAAALVGARRARLADPGRQKTSRA"
     gene            complement(3253..>3620)
                     /locus_tag="VIJ44_00145"
     CDS             complement(3253..>3620)
                     /locus_tag="VIJ44_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164335.1"
                     /translation="TQVGKGGGITYGWNQLTGSTDTPSGKTDVELLGNVDYLNGSGRF
                     FGFVTLKFASLSTLGFVMEGRAAKQAGGSTKLKAALKVIDGNAAFTGARGAGSFTGTR
                     AAALGSPIEITIKAKVRLS"
BASE COUNT          518 a         1194 c         1313 g          595 t
ORIGIN      
        1 tacgcgcacg acgggttata ggcgaagttc aggtcgatga ggagcgtggt cgtcccgttg
       61 gggtcgacgt ggccgccgag gtcggcgccc ttggcggtgt cgaggaggta gcggccaccg
      121 ccgtaggtct cgggattggg gtccttgacc gggaggaaga gccccccgcc gtatccgagg
      181 agccaccaca cgtcgaggtt cccgaagcca gggagccgcg cctgcccgac tcgtcggaag
      241 gtgacaatgc cgtcggtgcc ggtcgggacc tcgagggccg cggcggggct ggcggtgtcg
      301 atatcgaggg tgaatcgcag ggtcgggtcg tagggcgcgt agcgcagccc ggtgaaggcg
      361 gcgcggtcgg cggcgagcag cggtgacgcg ggatggtccg cgaacaggtg gtctcgcccg
      421 tggcgccaga tgatgtgccc ggttgcgggg tcgtcggtcg ctcgcacgtc ggtgtagagc
      481 tcggtgacgc ggcgacgcca gtcgagcacc gcgagcgaca cggccgaact gtacgcggcg
      541 tgcgccgggg tgccgacaga tggatcgtgg ttcagctagc gccggagacg gccggcgcga
      601 tctcgccgcg gccggcctgt tgcaggtctg agggcaggcg cgtgtgcatc tgacggctga
      661 tgacttcgag tcgggttcgc cactcgatga tggcggcgcg ttgggcggct tgggtgttgg
      721 ggagggtgga gaagtctcca ctggggagcg cggcggtcag tgcgcccagg tacgcgtctt
      781 cgaggatgta ggtgtgcgca tagggggcgt ggaggcccgc cagggtgggg ttcgccggcg
      841 tgagggcgtt gatctggacg gtgtaggacg cgaatcgtcg ctccagagtg cccatggccg
      901 cggcggcctg gtcgccggtt gactggtgct cgcgatacga ctcgaggatc gggtccgcgc
      961 cgtcgaggag gtcgttgacc ccgagtcgga tgggctcgac ggcgtgaacg tacgagccga
     1021 gggcgccgcc cgacacccgg ggcgagcggg tagacgccgc ggtcgcgccc agcgcagccg
     1081 cgacgacgac cgcgccgatc caatgcatga cctgagtctg ctccggtccg ggttactcag
     1141 gcggggcggg gacccgcggg cagcgcagca aaccccgcac cccgaggcga cgagccgtcc
     1201 ttgcggtgac cagagtcgtc ggtccatccc atcagaccgt ggcggcggtc ggtgcgtgaa
     1261 cggcggctcg ctccaggcgt ggtccggcga tactgcatgt gtgaccgcga tgccggcgac
     1321 ggggccaatc cgctttggga tccaggtggt ggccgccgat tcgaggtcgt cgctcgttga
     1381 gctcgcccgc cgcgcggaaa gtctcggttt cgacacgttg ctcgtccccg accatgtcgg
     1441 tcttcccgac ccgtttgtga ccttgcaagc ggcagcggac gcgaccacga cgctgcgggt
     1501 cgggacgtat gtgctcaaca acgacttcca cgacccggtg cttctcgcgc gcgccgcggc
     1561 gacgctcgac gtgctcagcg acggccgctt cgagctcggg ctcggcgcgg gccacgccca
     1621 gccggagtac gaggcgcgcg gcgtcgcgtt cgagcccgcg aagcaacgcg tcgctcggct
     1681 cgccgaggcg gtgcccctgc tccgtcggct cctcgacggc gagtccgtga cctccgaggg
     1741 tgtctactac cggcttcaca acgcggcctg tgcaccctct cctcgtcagg ctcgcgtccc
     1801 gatcgtcgtc ggtggcaacg gtgccgaggt gctccgactc gcggcgcggg aagccgacgg
     1861 tgccggtctc acgggtctgg gccgcacatt ggcggatggg gatcgccacg aggccgaatg
     1921 gtccaccgcg tcgctcgagg gacggctgca gatgatcgga gacgcggccg ggtcgcgcgg
     1981 ccagcccgag ctcagcgcac tggtgcaggt cgtcgcggtc actgacgatc gtggagacgc
     2041 ggcgcggcga gtcgcggagc gagtcccggg cctgaccgaa acggacgcac tggagacacc
     2101 attcctgctg ctcgggtcat tgccgcaaat ggtcgaacag atcgtggcgg cacaccagcg
     2161 gtggggcttc cactatttcg tgacccgagc cgactcggtg aacgtcatgg gggacgtcat
     2221 cgccgaagtt cacgcgcggt ccgatcccga gcggccgtga cggcggcatc cgctcgatcc
     2281 cactgctctc agcgttgcgc gccgacgcgc tccgccgccc gaactgattc ctccacggcc
     2341 ctcgcttctc cgggctggag attggcgcca acgaacgttg aggccgcggt cgtcgacgga
     2401 ctcgccgaac tgatttcccg cgacgacctc gctgagatcc gcgtcgctac acgcagcccg
     2461 atctgcgcgt cccgccgaat gtcttcagct tcgtcggtgg agttcggcga tctgtcacgc
     2521 tcgacccaga atttgtctcg ggcgccggac tcggcagggg acggtcgagc ttgagcttca
     2581 gttttggcgg catgctcacc tggcgagttc ggagccgccg gcgggcggct cgcctacgcc
     2641 cgcgacgtct tttgtcgccc cgggtccgcg aggcgtgctc gcctggcacc gaccagggcg
     2701 gccgcgccga ggctgatctc cgcggtcaga tacgcccaga acgcgccgtc ggttctgggc
     2761 tggtcgagca ccagcgagcc cacccgggct ccggctgccc ccagccagac gacgccgacc
     2821 gcggcctgcg cggtggggtc gcgactgacg agcgcccatc caccgagcgc ggcataggtt
     2881 ccaccgagcc cggctcgcgt ctccgtgatg ccccgtgcag tcacggcggg cagctcgagg
     2941 gctgaggcga ccctgctcgg catcacgacg ccggcgaggc ccgaagccat gagcgccgcg
     3001 ctgctccagc gtcccaattt cacgtcgtgc gggtcctcaa ctggtcgtgc gctcgtggtg
     3061 cgttgctccg tggagagacc cggccaatct accgggcctg atcatcgccg tcctcgccgt
     3121 catagcggcc atgaccgcct aagccgagca gagtcaatgg cgaagatcga gactgtgacg
     3181 gctgcgcgag tttgcgcagc tcaaaaccaa atgttctgcg tcgacgaccc tgcggatctg
     3241 aggatctaac cgctagctga gccgcacctt ggccttgatg gtgatctcga tcgggctgcc
     3301 cagcgccgcg gcccgcgtcc cggtgaacga gccggcacct ctcgccccgg tgaacgcggc
     3361 gttgccgtcg atcaccttga gtgcagcctt gagtttcgtg ctgccgccgg cctgcttggc
     3421 ggcccttccc tccatgacga agccgagcgt ggagagagac gcgaatttca acgtgacgaa
     3481 cccgaagaat cgtcccgacc cgttgaggta gtcgacgttg ccgaggagct cgacgtcggt
     3541 cttgcccgac ggcgtgtcgg tcgagccggt gagctggttc cacccgtagg taatgccgcc
     3601 gcctttgccc acctgcgtca
//
LOCUS       DATCCH010000008         3444 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_540801, whole genome shotgun sequence.
ACCESSION   DATCCH010000008 DATCCH010000000
VERSION     DATCCH010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540801"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1128
                     /locus_tag="VIJ44_00150"
     CDS             <1..1128
                     /locus_tag="VIJ44_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014214389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
                     /protein_id="HEY5164336.1"
                     /translation="DADPQATRPAPFDADAALVQLTSGTTGAPKPVVLRHGAVLALMD
                     AVVDTLRGGGSKHRRAPMPNLIPVSLSLWAGVYNVCFAFRVGAPVVLLERFEPSRFAA
                     LVARHEIRSVVLPPAAMTMLADDADLVSLAPLRLVRSITAPLSPFQARRFRARFGVDV
                     LNSYGQTELGGEVIGWSAGDLPVFGVTKLGAVGRPHTGVEVRIVDGELQVRTGRAVGT
                     DATGRADDDRLDPDGWFRTGDLARIDADGFVWIEGRVSEMINRGGLKVYPAEVEEVLR
                     AVPGVVDAAVVGAPDDRLGEVPVAFIVGLVDERLEARCRAELAPYKVPVRFVGVDALP
                     RTEVGKVIPGALLQQLTPDRELRLRSAVTGARLGTPPPDIG"
     gene            1163..1912
                     /locus_tag="VIJ44_00155"
     CDS             1163..1912
                     /locus_tag="VIJ44_00155"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008187946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5164337.1"
                     /translation="MTTALVTGASSGIGAELARLVAADGHDVVLVARRASELGALAAE
                     LSGARVIVADLADPASPAKLVAEVPDVDLLVNNAGVGDFGPFADADLAKTTAMIQLNV
                     TSLTELTRWYLPGMLDRGHGQVLNVASTAAFQPGPLMAVYYATKAYVLSFSEAIAEEL
                     RGSGVTVTALCPGPTASGFQTGAAMEGSRLVKDRRLPSAATVARAGHRALRRGDVVAV
                     VGARNKFLAASVRFTPRPVIRRLVGRMQRSD"
     gene            complement(1904..2293)
                     /locus_tag="VIJ44_00160"
     CDS             complement(1904..2293)
                     /locus_tag="VIJ44_00160"
                     /inference="COORDINATES: protein motif:HMM:NF022700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3054 domain-containing protein"
                     /protein_id="HEY5164338.1"
                     /translation="MTSDRVSRAGIAAALDGVCLVAFVVLGRGSHGLNGGSDWFVTVL
                     WPFVVGWYAAAFITGLYRAARHPWRRAAVTVALGVTLACVIRVAATSRSTPVVFGVVA
                     FSFLLLTTVGWRVAARLVTRVRVRASV"
     gene            complement(2283..3362)
                     /locus_tag="VIJ44_00165"
     CDS             complement(2283..3362)
                     /locus_tag="VIJ44_00165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEY5164339.1"
                     /translation="MSDGALEVAQPHRWSLGRVLLLAVTGICLYLLAPSIVEVFSAWE
                     RLDRFNPIWIMVIIACETASFACIWGLQRIVMPDVGWFDGAMTQLVGNAFNRITPGGG
                     ATGTALQARLLADAGHDLTSVAAALTTESLMVTLAITVLPLFSLPAILLGLNVPGSLA
                     DAAWIGVLVFVVVTLAGVWMLRSEQPLCRLGNGLAWIANKLRRRRAPIDGLGERLIIQ
                     RDTLREAVGSQWLAAVSAAVGRWVFEYIALLVALKAIGAQPDPTLLLLAFTTAELLGL
                     LPFTPGGLGFVEAGLTGTLALAGVGASDAVLATLVFRLVSFWLPLPVGLGASFAYRRR
                     HPRREGQGADAETDGSAGHTHPGDQ"
BASE COUNT          517 a         1230 c         1232 g          465 t
ORIGIN      
        1 gacgcggacc cgcaggcgac gcgtccggcc ccgttcgacg ccgacgccgc gctggtgcag
       61 ctgacgtcgg gcaccaccgg ggccccgaag ccggtggtgt tgcgccacgg cgcggtcctg
      121 gccttgatgg acgcggtcgt ggacaccctc cgcggcggtg ggtccaagca tcggcgggcg
      181 ccgatgccga acctgattcc cgtctcgctg tcgttgtggg ccggtgtcta caacgtgtgc
      241 ttcgcgttcc gggtgggtgc accggtcgtc ctgctcgagc ggttcgaacc gagccggttc
      301 gcggcgctgg tggcccgcca tgagatccga tcggtggtgt tgcctcccgc ggcgatgacg
      361 atgctcgccg atgacgccga cctggtgtcg ctggcgccgc tgcggctggt gcgcagcatc
      421 accgcaccgc tgtcgccgtt ccaggcccga cggttccgcg cccggttcgg ggtcgacgtg
      481 ttgaactcgt acggccagac cgagctcggc ggtgaggtga tcgggtggag cgccggtgac
      541 ttgccggtgt tcggggtcac caagctgggc gcggtcggtc gcccccacac cggggtggag
      601 gtgcggatcg tcgacggtga gctgcaggtg cgaaccggcc gtgccgtcgg caccgacgcg
      661 accggcagag ctgatgatga ccggctcgat cccgacggct ggttccggac cggtgacctg
      721 gcccgcatcg acgctgacgg gttcgtgtgg atcgagggac gcgtctcgga gatgatcaac
      781 cgcggcgggc tcaaggtgta cccggcggag gtcgaggagg tgctccgggc cgtgcccggc
      841 gtcgttgacg ccgcggtggt cggcgcgccc gacgaccggc tcggcgaggt cccggtcgcg
      901 ttcatcgtcg gcctcgtcga cgagcgcctc gaagcccgat gccgagccga gctcgccccg
      961 tacaaggtcc cggtccgatt cgtcggcgtc gatgcgctgc cgcgcaccga ggtcggcaag
     1021 gtgatcccgg gcgcgttgct ccagcagctc acgcctgatc gcgaactccg gttgcggtcg
     1081 gcggtgaccg gtgctcgact cgggacgcca ccgccggaca tcggctgacg gacacccgaa
     1141 ccccgaagcc gagaggggag ccatgaccac tgcgcttgtc accggcgcgt cgagcggcat
     1201 cggcgccgag ctcgcccggc tggtcgccgc cgacggccac gacgtcgtcc tcgtcgcgcg
     1261 ccgggccagt gagctcgggg ccctcgccgc cgagctcagc ggcgcgcggg tcatcgtcgc
     1321 cgacctcgcc gaccccgcca gtccggcgaa gctcgtggcc gaggtgcctg acgtcgatct
     1381 gttggtcaac aacgccggcg tcggtgactt cggaccgttc gccgacgcgg acctcgccaa
     1441 gaccacggcc atgatccagc tgaacgtcac gtcgctcacc gagctcaccc gctggtacct
     1501 gccgggcatg ctcgaccggg gtcacgggca ggtgttgaac gtcgcgtcga cggcggcgtt
     1561 ccaacccggt ccgctcatgg ccgtctacta cgcgacgaag gcctacgtgc tgtcgttcag
     1621 cgaggcgatc gccgaggagc tccgcgggag cggggtcacc gtcaccgcgc tgtgcccggg
     1681 gccgacggcg tccgggttcc agaccggtgc cgccatggag ggatcccggc ttgtgaagga
     1741 ccggcggctg ccgtcggccg cgacggtggc gcgcgccggg caccgggccc tccggcgtgg
     1801 cgacgtcgtc gccgtggtcg gcgcgaggaa caagttcctg gcggcgtcgg tgcggttcac
     1861 cccccgcccg gtgatccgtc gcctcgtggg acggatgcag cgctcagact gacgcccgga
     1921 ctcgcacccg ggtcacgagc cgcgccgcca cgcgccaacc gacggtcgtc aacaacaaga
     1981 agctgaacgc gacgacaccg aacaccaccg gcgtcgaacg gctcgttgcc gcgacccgga
     2041 tcacacacgc cagcgtgacg ccgagcgcga cggtcaccgc ggctcgtcgc cacgggtgcc
     2101 tcgctgcacg gtagaggccg gtgatgaacg ccgccgcgta ccagccgacc acgaacggcc
     2161 acagcacggt cacgaaccag tcgctcccgc cgttcagccc gtggctgcct cgaccgagga
     2221 ccacgaacgc gacgaggcac acgccgtcga gggctgcggc gatgccggcc cgcgaaaccc
     2281 ggtcactggt caccggggtg agtgtgcccc gccgacccgt ctgtctccgc atcggctcct
     2341 tggccctccc ggcggggatg gcggcgccgg tacgcgaacg acgcgccgag gccgaccggc
     2401 agcggcagcc agaacgagac cagacggaac accagcgtgg cgagcaccgc gtcggaggca
     2461 ccgaccccgg cgagcgccag ggtcccggtg aggccggcct ccacgaaccc gagcccgccc
     2521 ggtgtgaacg gcagcagacc gaggagctcg gcggtggtga aagccaacag gagcagggtc
     2581 gggtcgggtt gcgcgccgat ggccttcagc gcgacgagca atgcgatgta ctcgaacacc
     2641 caccgtccga ccgcggcgga gaccgccgcg agccactgcg acccgaccgc ctcgcgcagc
     2701 gtgtcccgct ggatgatgag ccgctcgccg agaccgtcga tcggcgcccg gcggcgccgc
     2761 agcttgttcg cgatccaggc cagcccgttc ccgaggcgac agaggggctg ttcgctgcgc
     2821 agcatccaga ccccggcgag cgtcacgacc acgaacacga gcaccccgat ccatgcggca
     2881 tcggcgaggc tgccgggcac gttgagaccc aagaggatcg ccgggaggct gaacagcgga
     2941 aggaccgtga tcgcgagggt gaccatcaac gattcggtgg tcagcgcggc ggcgacgctg
     3001 gtgagatcgt ggcccgcgtc agcgagcagg cgggcttgca acgcggtccc cgtcgcaccc
     3061 ccgccgggcg tgatccggtt gaaggcgttg ccgacgagct gggtcatcgc accgtcgaac
     3121 caaccgacgt cgggcatcac gatgcgttgc aaaccccaga tgcaagcgaa gctcgccgtc
     3181 tcgcaggcga tgatcaccat gatccagatc ggattgaacc ggtcgagccg ctcccacgcc
     3241 gagaagacct cgacgatgct cggggccagc agatagaggc agatgccagt cacggcgagg
     3301 aggaggaccc ggccgagcga ccaccggtga ggctgggcta cctccagagc accgtcgctc
     3361 accgcccgct ctccctctcg cgtctgcctc gccccgagca ggcatcggca tcgccccggt
     3421 cggggccgcc accgagagcc cggc
//
LOCUS       DATCCH010000009         2310 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_84822, whole genome shotgun sequence.
ACCESSION   DATCCH010000009 DATCCH010000000
VERSION     DATCCH010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84822"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1525)
                     /locus_tag="VIJ44_00170"
     CDS             complement(<1..1525)
                     /locus_tag="VIJ44_00170"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY5164340.1"
                     /translation="MSVDAPARHTADEKPARIPRPSWGAAVIAAVVLVGIALRFYTRS
                     DLWLDEALSVNIARLPLRQLHDALRHDGAPPLYYVVLHGWIRLFGTGDTAVRALSGLF
                     SVATLPLAWFAGKRLGGRPLAWLAVVVLATSPYAIRYGTESRMYALVMLLVFAGYLAV
                     LRAVERPTIARLAVVALVTAFLVYTQYWSFYLIAVAVLLLGWRALRGEPGPSRQAAWR
                     ALAAIVVGTLTFAGWIPTFLYQARHTGTPWGNARVPWSAIAEALLKFGGEDTHAETFI
                     LVFALITLVVLAVFGRGVDGRTIDLDLGTQPRIRREAILAFGTLLVGTTLSFVAGSAF
                     DARYASIMFPFFVLLVAIGLTLFVDPRVRAGVLVVVVLIGIIGGIRNVTTNRTQAAQV
                     TNIITAEAHPGDVVAYCPDQIGPSASRRLGAVPGLIQLTFPDGASPDRVNWVDYIDRI
                     RAASPQAFARRVVSRAGNHTIWYVSATGSNHVEGKCEALGAALTVARPKTTLRVVQDD
                     "
     gene            complement(1552..>2310)
                     /locus_tag="VIJ44_00175"
     CDS             complement(1552..>2310)
                     /locus_tag="VIJ44_00175"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY5164341.1"
                     /translation="SQRTTTIGLGQLVTCSSYRNAGLLAKEAAGLDVMSGGRLILGLG
                     AGWFDREYRAYGYEFRAARERLQILEETVQVIRALWTDETVTFDGRHLHFDGAYCDPK
                     PLQTLPPVWIGGGGEQVTLRIAAAFADATNWQVGLDEFVHKSEVLRGHCDRLGRDFDS
                     IVRTHGPDCRIFDTQRDLDAWLDAPDGGNLWGGGDPVVYVRDNFVGTVDQVAEKVQAF
                     VDAGCREFVLWFRDYPARDSLEAFATDVRPTIRG"
BASE COUNT          386 a          729 c          888 g          307 t
ORIGIN      
        1 agtcgtcctg gacgacccgc agcgtcgtct taggtcgggc gacggtgagc gccgcgccga
       61 gggcctcaca tttgccttcg acgtggttgg agccggtcgc ggacacgtac cagatggtgt
      121 ggttcccggc gcggctcacg acccgccggg cgaacgcctg ggggctcgcg gcgcggatgc
      181 ggtcgatgta gtcgacccag ttcacgcggt cggggctcgc gccgtcgggg aaggtgagct
      241 ggatcaggcc gggcaccgcg ccgagccggc ggctggccga tgggccgatc tggtcggggc
      301 agtaggcgac cacgtcaccc gggtgggcct cagcggtgat gatgttggtc acctgcgcgg
      361 cttgggttcg gttggtggtg acgttgcgga tccctccgat gatgccgatg agcacgacga
      421 ccaccaggac cccggcccgg acccgcgggt cgacgaagag cgtgagaccg atcgcgacga
      481 ggagcacgaa gaacgggaac atgatcgacg cgtagcgggc gtcgaaggcg ctcccggcga
      541 caaacgacag cgtggtgccg acgaggaggg ttccgaaggc gaggatggct tcgcggcgga
      601 ttcggggttg ggttccgagg tcgaggtcga tggtgcgtcc atcgacgccc cggccgaaga
      661 ccgcgaggac cacgagggtg atgagcgcga agacgagaat gaaggtctcg gcgtgggtgt
      721 cctctccccc gaacttgaga agcgcctccg cgatcgccga ccagggcacg cgggcgttgc
      781 cccacggcgt cccggtgtgt cgcgcctggt agaggaaggt ggggatccag cccgcgaagg
      841 tgagggtgcc gacgacgatg gcggcgaggg cgcgccacgc ggcctgccgg gacggaccgg
      901 gttcgccccg cagggcacgc cacccgagga gcagaacggc gacggcaatg aggtagaagg
      961 accagtactg ggtgtagacg aggaacgcgg tgacgagcgc gacgacggcc agccgtgcga
     1021 tggtgggtcg ctcgacagca cgcagcaccg cgaggtaccc ggcgaacacc aggagcatga
     1081 cgagcgcgta catgcgcgat tcggtgccgt aacggatggc gtacggagag gtggcgagga
     1141 ccaccaccgc gagccaggcc agcggacgcc caccgagacg cttcccggcg aaccaggcca
     1201 ggggcaacgt cgccaccgag aacaggcccg acagggcccg caccgcggtg tcaccggtgc
     1261 cgaagaggcg gatccagccg tggaggacca cgtagtacag cggcggggca ccgtcgtgtc
     1321 gcaacgcgtc gtggagctgg cgcagcggga gccgggcgat gttgaccgag agggcctcgt
     1381 cgagccatag gtcggaccgg gtgtagaacc gcagcgcgat cccgacgagg acgacagcgg
     1441 cgatgaccgc ggcgccccac gacgggcgtg ggatgcgcgc cggtttctcg tcggcggtgt
     1501 ggcgcgcggg cgcgtcgacg ctcacgagta ccgcacctta ggcgggcggg ctcagccgcg
     1561 gatggtcggg cggacgtcgg tggcgaaggc ttcgaggctg tcgcgcgccg ggtagtcgcg
     1621 gaaccacagg acgaactctc ggcagccggc gtcgacgaag gcctgcacct tctcggcgac
     1681 ctgatcgacg gtgccgacga agttgtcgcg gacatacacc accgggtcac ctcctcccca
     1741 gaggtttccc ccgtcgggtg catccagcca tgcgtcgagg tcgcgttgcg tgtcgaagat
     1801 gcggcagtcg ggaccgtggg tgcgcacgat cgagtcgaag tcgcgaccga gccggtcgca
     1861 gtggccgcgc agcacctcgg acttgtgcac gaactcgtcc aggccgacct gccagttcgt
     1921 ggcgtccgcg aacgcggcgg cgatccgcag cgtgacctgc tcgccgccgc cgccgatcca
     1981 gaccggcggg agggtctgga ggggcttcgg gtcgcagtag gcgccgtcga agtgcagatg
     2041 ccgaccgtcg aaggtgacgg tctcgtcggt ccagagcgcg cggatcacct ggaccgtctc
     2101 ttcgaggatc tgaagccgct cccgcgcggc gcggaactcg tagccgtacg cgcggtactc
     2161 gcggtcaaac cagccggcgc cgagaccgag gatcaaccgg ccgccgctca tgacgtccaa
     2221 ccccgcggct tccttggcga gcagccccgc gttgcggtac gaggagcagg tgacgagctg
     2281 gcccagcccg atggtcgtgg tgcgctggga
//
LOCUS       DATCCH010000010         5952 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_167638, whole genome shotgun sequence.
ACCESSION   DATCCH010000010 DATCCH010000000
VERSION     DATCCH010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5952
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167638"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VIJ44_00180"
     CDS             <1..322
                     /locus_tag="VIJ44_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008878784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY5164342.1"
                     /translation="ERVAESRIEIEQARLLTMKAAWLMDTVGNKGARFEIAAIKVVAA
                     RLATTVIDRAIQLFGGAGVSDDWPLAEMYTYARTLHLVDGPDEVHRQQIARRELRGYE
                     AFRT"
     gene            319..1212
                     /locus_tag="VIJ44_00185"
     CDS             319..1212
                     /locus_tag="VIJ44_00185"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY5164343.1"
                     /translation="MKVRVGYGLGVQGLRDEARFAQLVDSLERYRFDSLWLSERIAGD
                     APDPVVGLSFAAGRTERLKLGTSVQVLPGRNPVLLAKSWASLDRLSGGRTLPAFGLGN
                     PVAAEFQAFGVARDARASIFDEALPLIRRLWTEDGVDHDGPHFHYEALTVHPKPVQQP
                     PDVWLGGRAPSELRRCGRLGDGWLASFCTPADCEAGRPVIDEAASGVGRRVDPEHFGA
                     MVVYAHDEVPAALKDRLAVLRPGLDPADLVPVGLPALRERLIEFIGVGFSKLVLVPLA
                     EPDDWTAELEKVGAAVLDLQN"
     gene            complement(1236..2117)
                     /locus_tag="VIJ44_00190"
     CDS             complement(1236..2117)
                     /locus_tag="VIJ44_00190"
                     /inference="COORDINATES: protein motif:HMM:NF038012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY5164344.1"
                     /translation="MILTVVLALGAGLCYATAAVVQQRVAAQQPSELSLSVRLIVQLA
                     RRPLWLAGIAVDILAYLMEAAALGVGSVVVVGPLLASGLLFALPFASFRTGRRVTRRE
                     MIPALMVTAGLALYVGVGAPGGNASHASHLDWAIAGAGVVLGAGVAVFFGRRTKEPGR
                     RAIYYGLATGIVYSLTAVLTKATVDRIGSNVYPILGHWQLYALLIASAVGLVLNQSAF
                     QAGHVAASLPVISVTNPVLSSTMGILLFGEHLDAHGVAAWVVAGAAIIAMLVGTCWLA
                     RSPLVTEEVSVIPAMQV"
     gene            complement(2121..2768)
                     /locus_tag="VIJ44_00195"
     CDS             complement(2121..2768)
                     /locus_tag="VIJ44_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013732501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY5164345.1"
                     /translation="MRVTPALVGRGRPDHVALTFDDGPDPASTPAFLAELDRRGWNAT
                     FFMLGKMARRSPSLVGEVVAAGHEVAVHGDEHRNMLRRTPWGAREDIRRATDTIAELT
                     GRSPVWFRPPFGILSLGALRGARRAGLTTVLWTTWGRDWRKEATPSTVVADVLRRYVA
                     GGTVLLHDADGESYPGSWRSALGALAPLADAFAARDLTVGPLSEHGVARRTAARV"
     gene            complement(2867..4654)
                     /locus_tag="VIJ44_00200"
     CDS             complement(2867..4654)
                     /locus_tag="VIJ44_00200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY5164346.1"
                     /translation="MRRGILVISASMGAGHDGAARELERRLVARGHDVRVIDYLRLIP
                     FKLGGFVRWTYLFQLRVLPWTYDMTYKALGVGAPVLWGPVVRFMSLATRRALHRELLQ
                     SRPDAIVSTYPLASLVLGRMRKKGWLRVPVATYLTDFAVHPLWVHPGVDLHLAVSPLS
                     AETAAKRGGRENHAAGPLVGEHFRGSAMERPTSRQEMRRRLGIDDDERAILVVAGSWG
                     VGDVPDTVDAVCRAGPYHPITVCGRDDKLRASLIELGLGGTVIGWTDEMPALMSAADA
                     LVENAGGLTAMEAFAAGVPVVTFHPIAGHGKENAKTMATSGVSRYAHSADELEAALED
                     ATTPGPARDELINAGHALFAGDPTDHVEDLAADTRADALLIPFQLPKGRRRVSIAAVS
                     VAGLYLAMTIGAQGVSALGVGVAKPPKSATHDVFVGIRVNRAELANHQVLNAIDTLGV
                     TVVVDGRTAQRSGGQLAPLAADGIDIANGGWGQGRFLRWNRARDDCMKSWRTIAARSG
                     VSAHEFVPGRRLDGFDQIYCRSGRRKQRLVQANTTFGPETLPRPHGHAVYVLDGRNRD
                     PAAVATALENFASRVSHSHLHLRSLEGLR"
     gene            complement(4827..5852)
                     /locus_tag="VIJ44_00205"
     CDS             complement(4827..5852)
                     /locus_tag="VIJ44_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005630940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HEY5164347.1"
                     /translation="MARLEDPVAPRTRLVSRWRVAMTTGNAPAGAMDPVTRWLVLARA
                     SVLPMTVTAALFAVLLAWWNDEGHLDVGLWALAAVGIVLAHVANNLMNDLWDLKVGTD
                     SEQYPRALYAPHPVLSGMTTRRGLLLRALTVNVVDLAILAILVAARDPWIFFFALGGF
                     LLSVAYTAPPLRLKKHGLGEPTVFLVWGPLMVGGTYYAAVGHLPGAVVLASVPYALLC
                     TAVLMGKHIDKIPWDAPDGTHTLPVLLGERRARALTKAMMIAFYPLVGVLVAFGTLPI
                     LSLVCLGGIGGLAKVWKPFSEPKPDAPPAGFPIWPLWFAALAFVHTRRAGGLLVLGML
                     LGALVGV"
BASE COUNT          968 a         1996 c         2164 g          824 t
ORIGIN      
        1 agagcgtgtc gccgagagcc gcatcgagat cgagcaggcc cggctgctca ccatgaaggc
       61 ggcgtggctc atggacaccg tcgggaacaa aggtgcccgg ttcgagatcg cagcgatcaa
      121 ggtggtggcg gcccgtctgg cgacgacggt gatcgaccgg gccatccagc tctttggtgg
      181 cgccggcgtg tccgacgact ggccgctcgc cgagatgtac acctacgccc gcaccttgca
      241 tctggtcgac ggaccggacg aggtgcaccg tcagcagatc gcccgccgcg agctgcgggg
      301 ttacgaggcg ttccgcacgt gaaggtccgg gtcgggtacg gcctcggcgt ccaagggctt
      361 cgcgacgagg cccggtttgc tcagcttgtc gactcgttag agcgatatcg attcgattcg
      421 ctgtggctct cggagcggat cgcgggcgac gcccccgatc cggtcgttgg actgtcgttc
      481 gcggcgggcc gcaccgagcg gctgaagctg gggacgagcg tccaggtgct gccgggccgc
      541 aacccggtgc tgctcgcgaa gtcgtgggcc agtctcgacc gactctcggg agggcggacc
      601 ttgcccgcgt tcgggctcgg caacccggtc gcggccgaat tccaggcgtt cggcgtcgct
      661 cgcgacgcgc gggcgtccat tttcgacgaa gcgctccccc tgatccgtcg tctctggacc
      721 gaagacggcg tcgaccatga cgggccgcat ttccattacg aggccctcac cgtgcacccc
      781 aagccggtgc aacagccacc cgacgtgtgg ctcggcggtc gggcaccgag cgagctgcgc
      841 cgctgcgggc gacttggcga cggctggctg gccagcttct gcactcccgc cgactgcgag
      901 gcgggccggc cggtcatcga cgaggccgcg tcgggcgtcg ggcggcgcgt cgacccggag
      961 cattttgggg cgatggtggt ctacgcccat gacgaggtcc ccgctgcgct caaagatcga
     1021 ctcgcggtgc tgcgaccggg cctcgatccc gcggatctgg tgccggtggg actgccggcg
     1081 ttgcgcgagc ggctgatcga gttcatcggg gtgggcttct cgaagctcgt gctcgtcccc
     1141 ctcgccgagc ctgacgactg gaccgcggag ctcgagaagg tgggggccgc ggtcctcgac
     1201 ctccagaact gatcgccggt acagcgccgg tcgggtcaga cctgcatcgc ggggatgacc
     1261 gacacctctt cggtgaccag gggcgatcga gccagccagc aggtgccgac gagcatggcg
     1321 atgatggcgg cgccggcgac gacccatgcg gccacgccat gggcgtcgag atgctcaccg
     1381 aacaacaaga tccccatcgt cgagctgagt acggggttgg tcaccgagat cacggggagc
     1441 gacgccgcga cgtggccggc ctggaacgcg ctctggttga ggaccaggcc gacggcggag
     1501 gcgatcagca gggcgtagag ctgccagtgc ccgaggatcg ggtagacgtt gctcccgatg
     1561 cggtcgacgg tcgccttggt gagcaccgcg gtgaggctgt acacgattcc ggtggcgagg
     1621 ccgtagtaga tggcgcggcg ccctggttcc ttggttcggc gaccgaagaa gaccgcgacg
     1681 ccggcaccga ggacgacccc ggcaccggcg atcgcccagt cgaggtggga ggcgtgcgac
     1741 gcgttgccgc ccggcgcccc gaccccgaca tacaaggcca gtcccgcggt gaccatcaac
     1801 gccgggatca tctcgcggcg ggtcacgcgc cgaccagtgc gaaacgacgc gaacggcaac
     1861 gcgaagagga gcccgctggc caggaggggc ccaaccacga cgaccgagcc gacgccaagc
     1921 gcggcggctt ccatgaggta ggcgaggatg tcgacggcga tcccggcaag ccacagcggt
     1981 cggcgcgcca gctggacaat caggcgcacg gagagagaca gctccgatgg ttgctgcgcc
     2041 gcgacacgtt gctggaccac cgccgcggtc gcgtagcaga ggccggcccc gagcgcgagg
     2101 acgacggtga ggatcacggg tcagacgcgc gcggccgtgc ggcgcgcgac cccgtgttcg
     2161 ctgagcggac ccaccgtcag atcccgtgct gcgaacgcgt ccgcgagcgg ggccagggcg
     2221 ccgagcgctg atcgccacga gcccgggtac gactcaccgt cagcgtcgtg caacaggacg
     2281 gtcccacccg cgacgtagcg gcgcagcacg tcagcgacga ccgtcgacgg tgtcgcctcc
     2341 ttgcgccagt cgcgacccca ggtggtccac aggacggtcg tcagcccggc gcgtctggcg
     2401 ccgcggagcg cgcccagcga caggatcccg aacggcggcc ggaaccagac cgggctccgg
     2461 cccgtcagct cggcgatggt gtcggtcgcg cggcggatgt cctcccgggc gccccacggc
     2521 gtgcggcgca gcatgttgcg atgttcgtcc ccgtgcacgg cgacctcatg gccggcggcg
     2581 acgacctcgc ccacgaggga gggcgaccgg cgggccatct tgccgagcat gaagaaggtc
     2641 gcgttccaac ctcgccggtc gagctccgcc aggaacgccg gcgtggaggc cgggtctggg
     2701 ccgtcatcga aggtgagggc cacatggtcc ggtcgtccgc ggcccacgag ggcgggtgtc
     2761 acccgcatcc cgaacgggga gacccccgcg agcgccgggc cggcgtggag ggcgccggcc
     2821 gcgccagcca gacccgccgc ggagagggcg gcggcgatgg ccgcgctcag cgcaggccct
     2881 ccaaggaccg gaggtgcagg tgagagtggc tcacccgact cgcgaaattc tcgagcgcgg
     2941 tggcgaccgc ggcgggatca cggtttcggc cgtcgaggac gtagacggca tgaccgtgcg
     3001 gcctcgggag cgtctcggga ccgaaggttg tgttggcctg gaccagccgt tgcttgcgcc
     3061 ggccactccg gcagtagatc tgatcgaaac cgtcgaggcg ccgtccgggc acaaactcgt
     3121 gcgccgacac accggaacgg gccgcgatgg tccgccagga tttcatgcag tcgtcgcggg
     3181 cgcggttcca acgtaggaac cggccttggc cccagccacc gttggcgatg tcgatcccgt
     3241 cggcggcgag cggggcgagc tggcctcccg agcgctgggc ggtgcgaccg tcgacgacga
     3301 cggtcacccc gagcgtgtcg atcgcgttca ggacttgatg gttggccagc tcggcgcggt
     3361 tgactcggat cccgacgaag acgtcatggg tcgccgactt cggcggcttc gcgactccga
     3421 caccgagcgc ggagactcct tgggcaccga tggtcatcgc caggtatagg ccggcgacgg
     3481 acacggccgc gatcgacacc cgtcgccgtc ccttcgggag ctggaacggg atgagcagcg
     3541 cgtcggcgcg ggtgtctgcg gcgaggtcct cgacatggtc ggtcgggtcg ccggcgaaca
     3601 gcgcgtgacc ggcgttgatc agctcgtcgc gcgccgggcc gggtgtcgtg gcgtcttcga
     3661 gcgccgcctc gagctcgtcg gcgctgtgtg cgtagcggct cacccctgag gtggccatgg
     3721 tcttggcgtt ctctttcccg tgtccggcga tcgggtggaa cgtcacgacg gggacgccgg
     3781 cggcgaaggc ttccatcgcg gtgaggccgc cggcattctc gacgagggcg tcggcggctg
     3841 acatcagggc tggcatctcg tcggtccagc cgatgacggt cccgcccagc ccgagctcga
     3901 tcagtgacgc gcgcagcttg tcgtcgcgcc cgcagacggt gatcgggtgg tagggccccg
     3961 cccgacagac ggcatcgacg gtgtccggga cgtccccgac accccaggag cccgcgacga
     4021 cgaggatggc gcgctcgtcg tcgtcgatcc ccagccggcg tcgcatctct tgtcggctgg
     4081 tgggccgctc catcgcggag ccccggaaat gctcaccgac gagaggacca gcagcgtgat
     4141 tctcgcggcc gccccgcttg gctgcggtct cggccgagag cgggctgacc gccaggtgca
     4201 ggtccacgcc ggggtggacc cagagggggt ggaccgcgaa atcggtgagg taggtggcga
     4261 ccgggacccg cagccagcct ttcttgcgca tccggcccag gaccagcgac gccagcgggt
     4321 acgtcgagac gatggcgtca ggacgcgatt ggagcaactc ccggtgcaag gcccgccggg
     4381 tggccaggct catgaaccgg acgacgggtc cccacagcac cggggcgccg accccgaggg
     4441 ccttgtaggt catgtcgtag gtccagggca ggacccgaag ctggaagaga taggtccagc
     4501 ggacgaatcc gcccaatttg aacgggatca gccgcagata atcgatgacc cggacatcgt
     4561 ggccacgagc caccagccgg cgctcaagtt cccgcgcggc cccatcgtga cccgccccca
     4621 tcgacgccga gatgaccaga atccctcgac gcacaacttc cgacgatact cgccgggact
     4681 ccgatcgctt ggttgggatc catggacaaa acggcggaaa cccagcccac gcctggtccg
     4741 cgcgctctga ctgcggtcac ggggcggcga agggccggtc accctcggtg acgcacccga
     4801 tgctctgggt cgccgccgcg atcagctcag acgccgacga gcgcgccgag caacatgccc
     4861 agaacaagca ggcctccggc gcggcgagtg tggacgaacg ccagcgcggc gaaccacagc
     4921 ggccagatcg gaaagccggc cggcggcgca tcgggtttcg gttccgagaa cggcttccac
     4981 accttggcca ggcccccgat gccgccgagg cataccagag acaggatcgg cagggtcccg
     5041 aacgcgacga gcaccccgac aagcgggtag aaggcgatca tcatcgcttt ggtgagcgcc
     5101 cgggcccggc gttcaccaag gagcacgggg agggtgtggg tgccgtcggg ggcgtcccac
     5161 gggatcttgt cgatgtgctt gcccatgagc accgcggtgc agaggagtgc gtatggcacg
     5221 gaggcgagca cgaccgcacc cgggaggtgc ccgaccgcgg cgtagtaggt gccgccgacc
     5281 atgagcgggc cccacaccaa gaagacggtt ggttccccga gaccgtgctt cttcagtcga
     5341 aggggtggtg cggtgtaggc aaccgacaag agaaatccgc cgagggcgaa gaagaagatc
     5401 caggggtcgc gggcggcgac gaggatcgcc aggatcgcca ggtcaacgac gttgacggtc
     5461 aaggcgcgca gaagcagccc gcggcgggtg gtcatcccgg agagcacggg atgcggcgcg
     5521 tacagggccc ggggatactg ctcggagtcc gtcccgacct tgaggtccca aagatcgttc
     5581 atgaggttgt tcgcgacgtg ggcgagcacg atgcctacgg ctgcgagtgc ccagagcccg
     5641 acatcgaggt gaccttcgtc gttccaccac gccagcagca ccgcgaacag ggctgccgtc
     5701 accgtcatcg ggagaaccga ggcgcgcgcc agcaccagcc accgcgtcac cgggtccatc
     5761 gctcccgcgg gggcgttacc agtggtcatc gcgacccgcc accgagacac caatctggtg
     5821 cgcggcgcga ccggatcctc gagtcgagcc atgccgcgca gcgtactgat cccccaactg
     5881 atccgtccga cgtggcagga tttgcggcat tccgttcagg gggcccgcga gggggagacc
     5941 atgccggcca ag
//
LOCUS       DATCCH010000011         4734 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694 k141_997,
            whole genome shotgun sequence.
ACCESSION   DATCCH010000011 DATCCH010000000
VERSION     DATCCH010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4734
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_997"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..1183)
                     /locus_tag="VIJ44_00210"
     CDS             complement(140..1183)
                     /locus_tag="VIJ44_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_263500052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(767..769),aa:Sec)
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY5164348.1"
                     /translation="MSDTTATIVDDDRVAVVHATRDDDTLFVEADDLARATGWTLKPE
                     GLCRDDICVPVRDRGALVRDHHIDLRAFAAALSRPLALEPAAALAVLGEGSDTVGAQL
                     ASLSAPPFTLPDLDDNPVALADFAGRKRLLIAWASWUGCRWDLPVWQELYEELGPRGL
                     VPIAVALDEDLDAVKQWARDEPPAPLTYPMLVGRDHLVAERYGILNIPTTVWIDEDDR
                     IVRPPSIAPADDKFRDFTQIDSAVHHAALRSWVLDDVEPLTDGEIRARQIPPEPDLQL
                     ARAERRLAVHLLRAGDREAGERHLAAAMTLAPNDWTIRRGSMPIRGEDPFGQAFFDFY
                     QEWEAAGRPGYGA"
     gene            1217..2032
                     /locus_tag="VIJ44_00215"
     CDS             1217..2032
                     /locus_tag="VIJ44_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009954242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5164349.1"
                     /translation="MGLLADKTAIITGGGGGIGRGIAERFASEGAAVVVAELDPGRAE
                     ETVVSIRASGATAISVVVDVREERDVERLLQAALDERGRVDVLVNNVGHYGGARKAFH
                     HSTPQDWLDLYRVNLEHVLLCSRAVLGPMVEQGSGAIVNVSTIEAFRGIPTRAVYAAF
                     KAGITGLTKSLALEYATHGIRVNAMAPDVTETLQVPYSQWVRPEDEHLIPVWVPVGRF
                     GTPSDAAGVALFLASDLASFVTGTTVHVDGGTFAAGGWFRTEEGDWTNRPRHP"
     gene            complement(2153..2602)
                     /locus_tag="VIJ44_00220"
     CDS             complement(2153..2602)
                     /locus_tag="VIJ44_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014982225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY5164350.1"
                     /translation="MHDAVTVHIAAPPRRIWAVVSDVTRIGTYSPETFEAEWLDGAAS
                     PAAGARFGGHVKRNGKGPTCWTTCTVLDCVPGREFAFGVGPGDRPLNVWRYQLEPIGD
                     GTDVAESFELAKTPMLRVYWALLGWVRGRSNRDGMRTALERVKADVE"
     gene            2697..3329
                     /locus_tag="VIJ44_00225"
     CDS             2697..3329
                     /locus_tag="VIJ44_00225"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY5164351.1"
                     /translation="MSGPRHPTLARILLVRHGESTWNAEGRWQGHADPPLSATGAAQA
                     RAAAPALTGIDAVWCSDLIRARQTAELCAPTGLVLCPEPLLRERDVGEWTGLTRDVIE
                     ERFPGWIASGRRPDGWEGDGAVARRAWQALRRVVAALEPGAHAVVVSHGGLIRAVEAG
                     LGAPNRPVPNLGGVWLRHATGTFDLGEQAVLVDRDGRAAAASEPAVAGPE"
     gene            3431..>4734
                     /locus_tag="VIJ44_00230"
     CDS             3431..>4734
                     /locus_tag="VIJ44_00230"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEY5164352.1"
                     /translation="MTTPHPELAAEQRHIDQAYGRLAAMREAARRVVDGYADVGAGGT
                     HQARLERDAAADLTRRRLAALDIGSTPLCFGRLDLEAERGPEDGGDAGRVFYVGRIAV
                     TDDDQAPLIVDWRAPVSEPFYRATAVEPMDVIRRRHFLTRPGDGRELIGIDDEVFDQS
                     AVSASGFTVVGEGALFAALDRERTGRMGDIVATIQSEQDEAIRAPLAGILVVAGGPGT
                     GKTAVALHRAAYLLYTHRRRLGSQGVLVVGPNSIFLRYIDQVLPSLGEEDVQLSTMSG
                     LKPTYRALAEDTPEAARIKGDRRMARVIRRALQDREHPLPRDVVVIVDGNLLRVTRSA
                     SERVVGRARRRRGPHNAKRPGVVRAVVDQLRGQYRQALGAAAPDDPDWDRELDARLRR
                     LPEVRAALERMWPVLSGGELIHDLFSFPGLIRSAAGGVLDSG"
BASE COUNT          687 a         1593 c         1730 g          724 t
ORIGIN      
        1 cgcccgtcgg ttagatgaat tcgttccagc gcgccgtcgg cgacggcggt gcagaccagc
       61 gtgtcgtcac catcgctcgt cccggcgcac cacaccggac cctccgggaa gcgaatcccg
      121 gtggcgacga cctcggggtt cacgccccgt aaccggggcg gccagcggct tcccactcct
      181 ggtagaagtc gaagaacgct tgaccgaacg ggtcctcacc ccggatgggc atcgaaccgc
      241 gccgaatggt ccaatcgttc ggggcgagcg tcattgcggc cgcgaggtgg cgttcgccgg
      301 cttcgcggtc accggcgcgg aggaggtgga ctgcgaggcg gcgctcggcc cgggccagct
      361 gcaagtcggg ttccggcggt atctgacggg cgcggatctc gccgtcggtg agtggttcga
      421 cgtcgtcgag gacccagctc cgcagcgcgg cgtggtggac cgccgagtcg atctgggtga
      481 aatcacggaa cttgtcgtcg gcgggagcga tggacggggg tcggacgatc cggtcgtcct
      541 cgtcgatcca gaccgtggtc gggatgttca ggattccgta gcgttccgcg acgaggtggt
      601 cacggccgac gagcatcgga taggtgagcg gggccggggg ttcgtcacga gcccactgct
      661 tgacggcgtc gaggtcctcg tcgagggcga cggcgatcgg caccaaaccg cgcgggccga
      721 gttcctcgta cagctcctgc cacacgggca ggtcccagcg acagcctcac caggacgccc
      781 aggcgatcag cagccgcttg cggccggcga agtcggcgag cgccaccggg ttgtcatcga
      841 ggtcagggag ggtgaagggg ggcgcgctca gcgaggcgag ctgggcaccg accgtgtcgg
      901 acccttcgcc gaggaccgcg agtgccgccg ccggttcgag cgcgagcggg cggctgagcg
      961 cggccgcgaa cgcacgcagg tcgatgtggt ggtcccgaac gagcgcgccg cggtcacgga
     1021 cgggcacgca gatgtcgtcg cggcagagcc cttcgggctt gagcgtccag ccggtggcgc
     1081 gggcgaggtc gtccgcttcc acgaagagcg tgtcgtcgtc tcgggtcgcg tggacgaccg
     1141 cgacgcggtc atcgtcgacg atggtcgcgg tggtgtcgct catgggacca acctactggc
     1201 gtcgtaggct cgccgcatgg gtctcctcgc cgacaagacc gcgattatca ccggcggtgg
     1261 cgggggaatc ggtcgcggca tcgccgagcg gttcgcgtcg gagggcgcgg cggtcgtcgt
     1321 ggcggagctc gacccgggac gggctgagga gacggtggtg tcgatccggg cgtcgggggc
     1381 aacggcgatc agcgtcgtcg tcgacgtgcg cgaggaacgc gacgtggagc gcctgctcca
     1441 agccgcgctg gacgagcgcg gtcgggttga tgtgctggtc aacaacgtgg gccactacgg
     1501 cggtgcccgg aaggcgtttc accactccac cccgcaggac tggctcgatc tgtaccgggt
     1561 gaacctcgag cacgtcctgc tctgcagccg ggccgtcctc ggccccatgg tcgagcaggg
     1621 aagcggagcg atcgtcaacg tctcgaccat tgaagccttt cgggggatac cgacccgtgc
     1681 ggtgtacgcg gcgttcaagg ccgggatcac cggcctgacc aagagcttgg cgctcgaata
     1741 cgcgacccac gggatccgcg tcaacgcgat ggcaccggac gtgaccgaga cactgcaggt
     1801 cccctactcc cagtgggtac gacccgaaga cgagcacctg atcccggtgt gggtcccggt
     1861 cggccggttc gggaccccat ccgacgccgc gggggtcgcc ctgttcctcg cctccgacct
     1921 ggcgtccttc gtcaccggca ccacggttca cgtcgacggc ggaacgttcg cggccggggg
     1981 ctggttccgt accgaagagg gtgactggac gaaccgaccc cgccacccgt gatctcggca
     2041 cggacacccg agagtcacga acagcccgaa gccgtgaccg agcctcgagc tcagacggat
     2101 cgggacggtt tgcgcagtgg tcttcgcaga aggccaaccg gaaaccgggg cctcattcca
     2161 cgtcggcctt gacgcgctcg agggctgttc gcatgccgtc gcggttgctt cggccgcgga
     2221 cccaaccgag gagcgcccag tacacgcgga gcatcggggt cttggcgagc tcgaaggact
     2281 cggcaacgtc ggtgccgtcg ccgattggct ccaactggta acgccaaacg ttgagcggcc
     2341 ggtcgccggg gccgacaccg aaagcgaact cgcggccggg aacgcagtcc agcaccgtgc
     2401 acgtcgtcca gcacgtgggg cccttgccgt tgcgcttcac gtggccgccg aaccgggcgc
     2461 cagccgctgg gctcgcggcg ccgtcgagcc attctgcctc gaaggtctcg gggctgtacg
     2521 tgccgatcct ggtgacgtca ctcacgacag cccagatccg tctcggggga gcggcgatgt
     2581 gaaccgtgac cgcgtcatgc atgccttcac ccaattttga tccttccggc gctgtcttcc
     2641 tgccgtgcgc actgggtccg atctgagtgt gggcgtcgaa ccggtagcgt gccgccgtga
     2701 gcggtcccag acatcccacg ctcgcgcgca tcttgctggt ccgccatggc gagtcgacgt
     2761 ggaatgccga ggggcgctgg cagggtcatg ctgaccctcc gctctccgcg accggagctg
     2821 cacaggcgcg cgccgccgct cccgcgctga ccggcatcga cgcggtgtgg tgttcggacc
     2881 tgatccgggc gcgccaaacc gctgagttgt gcgcaccgac gggcttggtg ttgtgcccgg
     2941 agcccctgct gcgcgaacgc gacgtcggcg aatggaccgg cttgacccgc gacgtgatcg
     3001 aggaacgctt tccgggatgg atcgccagcg gccggcgccc tgacggctgg gagggcgacg
     3061 gggccgtcgc ccgacgggcg tggcaggcgc tgcgccgggt ggtcgccgca ctcgaaccgg
     3121 gtgcccacgc ggtcgtcgtg tcgcatggcg gcctgattcg cgccgttgag gctgggctcg
     3181 gcgccccgaa ccgcccggtg ccgaacctcg gcggcgtctg gttacgacac gcgaccggga
     3241 cgttcgacct cggggagcag gcggtgctcg tcgaccgaga cgggcgcgcc gccgcggcct
     3301 cggaaccggc agtggcggga cccgagtaac gcggcccccg ggtgcgacga tgacaccgca
     3361 cggggatgtc actctcggcc agtaaagtgg tcggtccccc gttgtcgtgt gaacgtgagc
     3421 cggcgtcccg ttgaccactc cgcacccaga gctcgctgcc gagcagcgac acatcgatca
     3481 ggcctacggc cgcctcgccg ccatgcgcga ggccgcccgc cgcgtcgtcg acggctacgc
     3541 cgacgtcggg gccgggggca cccaccaggc tcgcctggag cgggatgccg ccgccgacct
     3601 gacccgccgc cgcctcgcgg cccttgacat cggctcgact ccgttgtgct tcgggcgtct
     3661 cgacctcgaa gcggaacgcg gccccgagga cggaggcgac gccggccggg tcttctatgt
     3721 cggtcggatc gcggtcaccg acgacgacca ggcgccgctg atcgttgact ggcgggcacc
     3781 ggtgtccgag cccttctatc gggccacggc cgtggagccg atggacgtca tccggcgccg
     3841 gcacttcctg acccgacccg gcgatggacg cgagctcatc ggcatcgacg acgaggtgtt
     3901 cgaccagtcc gcggtgtcag cgtcggggtt cacggtcgtc ggcgaggggg cgctcttcgc
     3961 ggcgcttgac cgggagcgca ccggacgtat gggcgacatc gtcgccacga tccaatccga
     4021 acaagacgaa gcgattcgtg cccccctggc ggggatcctg gtggtggccg gcggccccgg
     4081 cacggggaag accgcggtgg cgttgcaccg cgccgcgtac ctcctctaca cccaccggcg
     4141 ccgtctcggc tctcaggggg tgctggtcgt gggtccgaac agtatcttcc tgcggtacat
     4201 cgaccaggtg ctgccgtcac tcggcgagga ggacgtacag ctgtcgacga tgagtggact
     4261 caagccgacc tatcgggctc tcgccgagga cacgccagag gcggcgcgga tcaagggtga
     4321 ccggcgcatg gcccgtgtca tccggcgggc cctgcaggac cgggagcatc cgctgccacg
     4381 cgacgtggtc gtgatcgtcg atggcaacct gctccgcgtc acccggtccg catcggagcg
     4441 ggtcgtgggg cgagcccgcc ggcgtcgcgg tccgcacaac gccaaacggc ccggggtggt
     4501 ccgagcggtc gtggaccagt tgcggggtca gtaccggcag gccttgggtg cggcggcgcc
     4561 cgacgatccc gactgggatc gtgaactcga cgcccgcttg cgtcgcctgc ccgaagtgcg
     4621 agccgcgctg gagcggatgt ggccggtgct gtcgggcgga gagctgatcc acgacctgtt
     4681 ctcgttccct ggtctgatcc gttcggccgc cggcggcgtg ttggacagcg gcga
//
LOCUS       DATCCH010000012         6898 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_125331, whole genome shotgun sequence.
ACCESSION   DATCCH010000012 DATCCH010000000
VERSION     DATCCH010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6898
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125331"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1193
                     /gene="groL"
                     /locus_tag="VIJ44_00235"
     CDS             <1..1193
                     /gene="groL"
                     /locus_tag="VIJ44_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006133870.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEY5164353.1"
                     /translation="ISAADDEIGAMIADAIDKVGKDGVITVEESQTFGMELDLVEGMR
                     FDKGYISPYFVTDPERMEAVLDDAYLLLVSSKISAVKDMLPVLEKVMQGGKPLAIVAE
                     DVEGEALATLVVNKIRGTFKSVAVKAPGFGERRKAMLQDIAILTGGQVVSEEVGLKLE
                     NVGLDLLGTARKIVVTKDETTIVEGGGSKTDVQGRINQIKAEIDNTDSDYDREKLQER
                     LAKLSGGVAIIKVGAATEVELKEKKHRIEDAVQTTKAAVEEGVVAGGGVALVRAQAKV
                     LDASKKMDGDEATGARMVARALEEPIKQIAHNAGMEGGVVVEKVKNLSGPEGLNAATG
                     DYEDLIKAGVIDAAKVTRSALQNAASIAALFLTTEAVVADKPEEKPAMPAGMPGGGMG
                     DMDF"
     gene            1309..1749
                     /locus_tag="VIJ44_00240"
     CDS             1309..1749
                     /locus_tag="VIJ44_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164354.1"
                     /translation="MEEARIRSIVLAKWSDYERAVPAVRVRFLARESYLEAARRDQRV
                     LDELAVGWFSDFDTEFRDPVLADPVTEQVTVCPPIMRDVVAPEPPRIQRAFVEGAFLR
                     RLFRLLVETEGWEADAQVRAVMARHFPFQLVAVEAVEGRPGAGA"
     gene            1837..2628
                     /locus_tag="VIJ44_00245"
     CDS             1837..2628
                     /locus_tag="VIJ44_00245"
                     /inference="COORDINATES: protein motif:HMM:NF018484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HEY5164355.1"
                     /translation="MSDEPLEASVGWGVVHLFYRVDRARADDEPGSAKHVLEAIDRLT
                     ADDTHQVLTFAALGHKADLGVMAVGPDLARLQTFEQDLARAPLVPTWSYVSLTEQYEY
                     AATEEDERARLASDEGVTDPDELDTRLAVWRDRIEHYREQRIHPHLPARRAICFYPMS
                     KRRSGPDNWFALDFDDRKRLMAGHARVGRSYAGRVLQLITGSTGLDDWEWGVTLFADD
                     PAALKEIVYEMRFDEVSARYAEFGPFVTGLILEPVEALRRVGLDA"
     gene            2802..3116
                     /locus_tag="VIJ44_00250"
     CDS             2802..3116
                     /locus_tag="VIJ44_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164356.1"
                     /translation="MDAATGRVAAELADLIDTEPAAQRSTPLEIVRGAVLEPSVVLED
                     AGIPAVVRDEFEERSFPDDRYRLAPRTFADLDDALGPVQLVWGLAKAALLRALPSTDP
                     PQ"
     gene            3153..5462
                     /locus_tag="VIJ44_00255"
     CDS             3153..5462
                     /locus_tag="VIJ44_00255"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY5164357.1"
                     /translation="MTDLVTPAETARADQDQLCLEDESSLPLAHGGLSADQLGVVLQY
                     SFDTITVLDPDGTVRYSTPAAQRISGYPDAFDFGQSGFDRIHPDDVGDVLEAFTECLR
                     TPGLSRQVQLRLRHADGHWITMQAVGNNLVDDPSIHGIVISARDVTLQLQTEEALRTS
                     EGRLRDQAHILEMIATSEPLEDILAEICRSVEAYSTETRCTIWLVDEDATVLRPGAAP
                     SFPDSFTGALDGLPVAEGSAVCGTAAFRAETVVIVDALDDPMTEPLRDLARTEGIRGC
                     WSTPVRAASDGRVLGTFATYLREPRGPTPAEEATVESLLQLAAIAIEHHVFEDQLSHQ
                     AHHDPLTGLPNRTLFYELLEHALARSVRVNTAVAVLFLDLDRFKVVNDSLGHDAGDAL
                     LAVLARRLESVLRPGDVVSRFGGDEFTVLCEDVDPGTAGRQSVNVAERIIDAICEPFV
                     LDGDEQFLSASVGIALAFHGSERPEDLLRDADAAMYRAKERGRGCCEIFDEAMRARAQ
                     DRHEIENTLHRAVSREELRVFYQPVISLYDGECIGVEALVRWQHPDRGLLAPREFIPL
                     AEETGLVVPMGRWILNAAAAQAAEWQRAQREPARFRLAVNLSGRQLQDPDLAATVGEL
                     LDAVGLDPQALCVEITESVLMEDADVGVGALKALKSIGVRVSVDDFGTGYSSLGYLRR
                     FPVDEVKIDRSFVARLGTDPADAAIVAAVVSLGHALGVSVVGEGVETEAQLDELRELG
                     ADAAQGFLFAPPQPAADLTPRLLRGRGWG"
     gene            5547..6227
                     /locus_tag="VIJ44_00260"
     CDS             5547..6227
                     /locus_tag="VIJ44_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HEY5164358.1"
                     /translation="MSEPAPISAADLLRWAETLSGIARTGLGFTQSLYERERFEEVLK
                     VAADIRAGAVEEAEADALFDEWISTVGDGVAGYVTPKVAVAAVVGNDQGEILLTQRSD
                     SGVWLYPVGWADVGYSPSEVAVKEVLEETGIQVEPASLIAVFDGLRLGFARLPLYSLV
                     FHCRMLGGELRGHPLETRAVGFFARDALPWPLAGGARWIETAFAAIGGEQRPCDFDPP
                     RTPPWREG"
     gene            complement(6224..6751)
                     /gene="pyrE"
                     /locus_tag="VIJ44_00265"
     CDS             complement(6224..6751)
                     /gene="pyrE"
                     /locus_tag="VIJ44_00265"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019074201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEY5164359.1"
                     /translation="MDRAELARAIYAASHLTGTFTLRSGTTSHEYFDKYRFESDPELL
                     HAIGEALVPLVPDGTEALAGLELGGVPLAVALSSVTGLPALFVRKEAKTYGTCQLAEG
                     GELEGRRVTVVEDVVTSGGQVLLSCADLRDRGATVEYALCVIDREAGGPAALHDIGVE
                     ARPLFTMSELKAAVA"
BASE COUNT         1054 a         2508 c         2324 g         1012 t
ORIGIN      
        1 cgatctccgc ggcggacgac gagatcggcg ccatgatcgc cgacgcgatc gacaaggtcg
       61 gcaaggacgg cgtcatcacc gtcgaggaga gccagacctt cgggatggag ctcgacctcg
      121 tcgaggggat gcgcttcgac aagggctaca tctcgccgta cttcgtgacc gaccccgagc
      181 ggatggaagc cgtcctcgac gacgcctacc tgctgctcgt gtcctcgaag atctcggcgg
      241 tcaaggacat gctgccggtc ctcgagaagg tcatgcaggg cggcaagccc ctcgccatcg
      301 tcgccgaaga cgtcgaaggc gaagcgctcg cgacgttggt cgtgaacaag atccgcggca
      361 cgttcaagtc ggtggcggtc aaggcccccg gcttcgggga gcgccgcaag gcgatgctcc
      421 aagacattgc gatcctcacc ggcggccagg tggtctccga agaggtcggc ttgaagctgg
      481 agaacgtcgg cctcgacctg ctcggcacgg cccgcaagat cgtcgtcacc aaggacgaga
      541 ccaccatcgt cgaaggtggc ggctcgaaga ccgacgtgca gggccgcatc aaccagatca
      601 aggccgagat cgacaacacc gactccgact acgaccgcga gaagctccag gagcgcctcg
      661 ccaagctgtc gggcggcgtg gcgatcatca aggtcggtgc tgccaccgag gttgagctca
      721 aggagaagaa gcaccgcatc gaggacgccg tccagaccac caaagccgcg gtcgaagaag
      781 gcgtcgtcgc cggaggcggt gtggccctcg tccgagccca agccaaggtg ctcgacgcct
      841 ccaagaagat ggacggtgac gaagcgaccg gcgcccgcat ggtggcccgc gccctcgaag
      901 agccgatcaa gcagatcgcg cacaacgccg gcatggaagg tggcgtcgtc gtcgagaagg
      961 tcaagaacct gagcggcccc gaaggcctca acgccgccac cggcgactac gaggacctca
     1021 tcaaagccgg ggtcatcgac gcggcgaagg tgacgcgctc ggcgctgcag aacgcggcgt
     1081 cgatcgcggc gctgttcctc accaccgagg ccgtcgtcgc cgacaagccc gaggagaagc
     1141 ccgccatgcc cgccgggatg cccggtggcg gcatgggcga catggacttc tgacctcgaa
     1201 cccgagacca accgccggtg tggggcgccc ttcggggcgc cccacccgcg tccaccctct
     1261 ttcgcctgga tggtctcggg cgcccgccgc ggggtccaca ctggaggcat ggaggaagcg
     1321 cggatccgct ccatcgtcct cgccaagtgg tccgactacg aacgcgccgt ccccgcggtc
     1381 cgggtccgct tcctcgcccg cgagtcgtac ctcgaagccg cccgtcgcga ccagcgggtc
     1441 ctcgacgagc tcgccgtcgg ctggttcagc gacttcgaca cggagttccg cgacccggtg
     1501 ctcgccgacc cggtcaccga gcaggtcacc gtctgtccgc cgatcatgcg cgacgtcgtc
     1561 gctcccgaac cgccccgcat ccaacgcgcc ttcgtcgaag gggccttcct ccgccgcctg
     1621 ttccggctcc tcgtcgaaac ggaggggtgg gaagccgacg cgcaggtccg ggccgtcatg
     1681 gcccggcact tcccgttcca gctcgtcgcg gtcgaggccg tcgagggtcg gcccggcgct
     1741 ggcgcgtaga gtgaatcacc gcgcgtcgcg accgcggcgc cggacgacca agccctcgtc
     1801 gagcggctga catccgaacc cgagtgtgca ggacccatga gcgacgaacc cctcgaggcg
     1861 tcggtgggct ggggtgtcgt gcacctcttc taccgggtcg accgggcccg ggccgacgac
     1921 gagcccggga gcgccaaaca cgtcctcgag gccatcgacc ggctcaccgc cgacgacacc
     1981 caccaggtgc tgaccttcgc ggccctgggc cacaaggcgg acctcggggt catggccgtc
     2041 ggtcccgacc tggcccgact ccagacgttc gagcaggacc tggctcgcgc cccactcgtc
     2101 ccgacctggt cgtacgtgtc gctcaccgag cagtacgagt acgccgccac cgaggaggac
     2161 gagcgtgccc gcctcgcgag tgacgaaggc gtcaccgacc ccgacgagct cgacacccgc
     2221 ctcgcggtgt ggcgcgaccg catcgagcac taccgcgagc agcggatcca cccccacctt
     2281 cccgcccgac gggcgatctg cttctacccg atgtcgaagc gacgcagcgg acctgacaac
     2341 tggttcgcgc tcgacttcga cgaccggaag cggctgatgg ccgggcacgc ccgcgtcgga
     2401 cgctcctacg ccgggcgggt cctccagctc atcaccggtt ccaccggcct cgacgactgg
     2461 gagtggggcg tcacgctgtt cgccgacgac cccgcggcgt tgaaggaaat cgtgtacgag
     2521 atgcgcttcg acgaggtgtc ggcccgctac gccgagttcg ggcccttcgt caccggtctc
     2581 atcctcgagc cggtcgaggc gctccgccgc gtagggctcg acgcctgacc gacgagcacg
     2641 ccgcccgggc ccgcctcacc gaagccggcg cggccatcgt cgctggcgtc gaggcgcacc
     2701 tccccgagta tctcctcggt cgcaccgccc ggatcctcga cgcgtgggac cgtctccccg
     2761 gtgagacccc cgccggggtc gacgaacgtc tccggggcgc cgtggacgct gccaccgggc
     2821 gcgtcgcggc cgagctcgcc gatctgatcg acaccgaacc ggcggcgcaa cgctccacgc
     2881 cgctcgagat cgtgcgcggc gcggttctag agccgagcgt cgtgctcgaa gacgccggga
     2941 tccccgccgt cgtgcgcgac gagttcgaag aacgctcgtt tcccgacgac cgctaccgcc
     3001 tcgcgcctcg caccttcgcg gacctcgacg acgccctcgg cccggtgcag ctcgtctggg
     3061 gcctggcgaa ggccgccctg cttcgggctc ttcccagcac cgacccgcca cagtagagtg
     3121 gtcggtcggt caccgtgggt gagggatttg ggatgacgga ccttgtgacg ccggccgaga
     3181 cggcgcgggc ggaccaggac cagctgtgcc tcgaggacga gtcgagcctg ccgctcgctc
     3241 acggaggcct gtcggccgac cagctcgggg tggtccttca gtactccttc gacaccatca
     3301 cggtgctcga ccccgacggg acggtccgct acagcacccc cgcggcccag cggatcagcg
     3361 gctatcccga cgcgtttgat ttcgggcaga gcggcttcga ccggatccat cccgacgatg
     3421 tcggcgacgt cctcgaggcg ttcaccgagt gcctccggac ccccgggctg agccgccagg
     3481 tgcagctccg gctgcgccac gccgacggcc actggatcac gatgcaagcg gtcggcaaca
     3541 acctggtcga cgaccccagc atccacggca tcgtcatctc ggcccgcgac gtcaccctcc
     3601 aacttcagac cgaagaagcg ttgcgcacca gcgaagggcg cctacgcgac caggcgcaca
     3661 tcctcgagat gatcgccacc agtgagccgc tcgaagacat cctcgccgag atctgccggt
     3721 cggtggaggc ctactcgacc gagacgcgct gcacgatctg gctcgtcgac gaggacgcca
     3781 cggtgttgcg ccccggcgcg gccccgtcgt tccccgactc gtttaccgga gcgctcgacg
     3841 gcctgcccgt cgccgagggc tccgccgtct gcgggaccgc agcgttccgg gccgagacgg
     3901 tcgtcatcgt cgacgcgctc gacgacccca tgaccgaacc gctccgcgac ctcgcccgca
     3961 ccgaggggat ccgcggctgc tggtcgacac cggtgcgggc cgcgtcggac ggccgggtcc
     4021 tggggacgtt cgccacctat ctgcgcgagc cgcggggccc gactcccgcg gaggaggcga
     4081 ccgtcgaatc gctgctgcag ctggccgcaa tcgcgatcga gcatcacgtc ttcgaggacc
     4141 agctgtcgca ccaggctcat cacgatcccc tgaccggcct gcccaaccgg accctgttct
     4201 acgagctgct cgaacatgcc ctcgcccgat cggtgcgggt caacaccgcg gtcgcggtcc
     4261 tgttcttgga cctcgaccgg ttcaaggtcg tcaacgacag cctcggccat gacgccggcg
     4321 acgcgctcct cgcggtcctc gcccgacggc tcgagagcgt gctccgcccc ggggacgtcg
     4381 tctcccggtt cggtggggac gagttcaccg ttctctgcga ggacgtcgat cccggcaccg
     4441 cgggtcgcca atcggtcaac gtcgccgagc ggatcatcga cgcgatctgc gagccgttcg
     4501 tgctcgacgg cgacgagcag ttcctcagcg ccagcgtcgg gatcgccctc gccttccacg
     4561 gctcggaacg tccggaggat ctcctgcgag acgcggatgc cgccatgtac cgggccaagg
     4621 aacgggggcg gggctgctgt gagatcttcg acgaagcgat gcgggcccgc gcccaggacc
     4681 gccacgagat cgagaacacg ctgcatcgcg cggtgtcgcg cgaggagctg cgcgtcttct
     4741 accaaccggt catctcgctc tacgacggcg aatgcatcgg tgtcgaagcc ctcgtccgct
     4801 ggcagcaccc cgaccggggg ctgctcgccc cccgagagtt catccccctc gccgaagaga
     4861 ccgggctggt cgttcccatg ggccggtgga tcctgaacgc ggccgcggcg caggccgccg
     4921 agtggcagcg ggcccagcgg gaaccggccc gcttccggct ggcggtgaac ctctcgggcc
     4981 gccagctcca agatcccgat ctcgccgcaa cggtcggcga gctcctcgat gcagtcggct
     5041 tggatcccca ggccctctgc gtcgagatca ccgagagtgt gctgatggag gacgccgacg
     5101 tcggcgtcgg tgcgttgaag gcgctgaagt ccatcggcgt gcgagtcagc gtcgacgact
     5161 tcgggaccgg ctactcgtcg ctcggttact tgcggcgctt ccccgtcgac gaggtcaaga
     5221 tcgaccgctc gttcgtcgct cgtctcggca ccgaccctgc cgatgccgcg atcgtcgccg
     5281 cggtcgtgag cctcggtcac gcgctcgggg tctccgtcgt cggtgagggc gtcgagaccg
     5341 aagcccagct ggacgagcta cgcgagctcg gcgccgacgc ggcccagggc ttcctcttcg
     5401 cgccgcccca acccgcggcg gatctcaccc cccgtctgct ccgcggccgc ggctggggat
     5461 agcgctcgag gcgcgtcggg cgccgacttc gcggcgacga ggcgcacccg cgatcggaac
     5521 cggcaccctt cacctaaggt gcgcgtgtga gtgagcccgc cccgatctcg gctgcggacc
     5581 tgctgcgctg ggccgagacc ctgtcgggga tcgcgcgtac cggtctgggc ttcacccaga
     5641 gcctctacga gcgtgaacgt ttcgaagagg tgctgaaggt cgcggccgac attcgggcgg
     5701 gcgcggtcga agaagccgag gccgacgcgc tcttcgacga atggatctcg accgtcggtg
     5761 acggcgtcgc cgggtacgtg acaccgaagg tcgcggtcgc ggccgttgtt ggcaacgacc
     5821 agggcgagat cctcctcacc cagcggtccg actccggggt gtggctctac ccggtcgggt
     5881 gggccgacgt cggctactca ccctcggagg tcgcggtcaa ggaggtgctc gaggagaccg
     5941 ggatccaagt cgagccggcg tcgctcatcg cggtgttcga cggtctccgc ctcggcttcg
     6001 ctcgcctgcc gctgtactcg ctggtctttc actgccggat gctcggtggt gagctgcgag
     6061 gccatcccct cgagacgcgc gccgtcggct tcttcgcccg cgatgccttg ccctggccgc
     6121 tcgccggtgg cgcccgctgg atcgagactg ccttcgcggc gatcggcggc gagcagcgac
     6181 cgtgtgactt cgatccgccc cgcaccccgc cgtggcgaga gggttaggcg accgcggcct
     6241 tcagctcgct catggtgaac agcggccggg cctcgacccc gatgtcgtgg agcgcggcgg
     6301 gacccccggc ctcgcggtcg atgacacaca gcgcgtactc gaccgtcgcg ccccggtcgc
     6361 gaaggtcggc gcaggacagc aggacctgac cgcctgacgt cacgacatcc tcgacgacgg
     6421 tgacccggcg gccctcgagc tcaccgccct cggccagctg gcaggtgccg taggtcttgg
     6481 cctccttgcg cacgaacagc gccggaagcc cggtgaccga tgagagcgcg accgccaacg
     6541 gcaccccgcc gagctcgagc ccggcgaggg cctcggtccc gtccgggacg agtgggacga
     6601 gcgcctcgcc gatcgcgtgc agcagctccg ggtcgctctc gaagcggtac ttgtcgaagt
     6661 actcgtggct cgtggtaccc gatcgcagcg tgaacgtccc ggtgaggtgc gacgcggcgt
     6721 agatcgcccg ggcgagctcg gctcggtcca tcacggccga cgcccgagtt cgtcgagcag
     6781 catcccggtg atcgacgcgc cgcgcttgga gcgctcccac cggtcgccga tgccggcatg
     6841 gtccacgagt ccggcggcgt cgggggcgat gccgagcgcc tcggtcgcgg acgcgtcg
//
LOCUS       DATCCH010000013         8190 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_458402, whole genome shotgun sequence.
ACCESSION   DATCCH010000013 DATCCH010000000
VERSION     DATCCH010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 8190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8190
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458402"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /gene="rpsK"
                     /locus_tag="VIJ44_00270"
     CDS             <1..338
                     /gene="rpsK"
                     /locus_tag="VIJ44_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012785188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEY5164360.1"
                     /translation="GVAHIKSSFNNTIITITDQQGNALAWASSGNVGFKGSRKSTPFA
                     AQLAAETVARRAMEHGVRKVDVLVKGPGSGRETAIRTIQNTGIEVVGIKDVTPIPHNG
                     CRPPKRRRV"
     gene            342..965
                     /gene="rpsD"
                     /locus_tag="VIJ44_00275"
     CDS             342..965
                     /gene="rpsD"
                     /locus_tag="VIJ44_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011602317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEY5164361.1"
                     /translation="MARYTESVCKLCRRERMKLFLKGAKCDTMKCPVERKPYPPGEHG
                     RGRIRESEYLLQLREKQKARRIYGLLERQFRNLYQQASREPGITGETLLRMLEQRIDN
                     VVFRTNFAASRNQARQFVRHGHVLVNGKRVTIPSYRVRPGDAIAIGPKVKDNIIIRHN
                     IDTIDRAVPAWLDVSSDRLEATVRDVPLREQIDVPVREQLIVELYSK"
     gene            1020..1955
                     /locus_tag="VIJ44_00280"
     CDS             1020..1955
                     /locus_tag="VIJ44_00280"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEY5164362.1"
                     /translation="MLIIQRPEVEAGEPDGNTQSFIISPLEPGFGHTLGNSLRRTLLS
                     SIPGAAVTQVRFDEALHEFDTIPGVKEDVTDLILNIKDLVLRCSSADPVTLRLDKRGP
                     GDVTAGDLQTTSDVEVLNSELHVATVNAKGRLAIDLTVEQGRGYLSAERNKRTSTIGV
                     IPVDAIFSPVRRVAFSIEPTRVEQATNYDKLTIEIETDGSITPREALASAGDTLRSLV
                     GLVADLSDTPQGLELGEVSPATAASPDLELPIEELDLSERPRNCLKRARVDTIGQLAQ
                     KTEDDLLAITNFGSKSLEEVLQKLDERGLSLRVKE"
     gene            1960..2307
                     /gene="rplQ"
                     /locus_tag="VIJ44_00285"
                     /pseudo
     CDS             1960..2307
                     /gene="rplQ"
                     /locus_tag="VIJ44_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019610101.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
     gene            2307..3107
                     /gene="truA"
                     /locus_tag="VIJ44_00290"
     CDS             2307..3107
                     /gene="truA"
                     /locus_tag="VIJ44_00290"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEY5164363.1"
                     /translation="MTLFDAGTPASPHRETGSRLKLLIAYDGTDFHGFASQRVVRTVE
                     GVLTDALEKVLGRPSASLELVCAGRTDKGVHARGQVVSVEASPGTDCAEVHQAVNRMI
                     GPEVVVRCVELADDDFDARRSARWRRYRYTIVNGSEPDPFLARFAWWVPGPLDLRMLR
                     LAADPFVGDHDFAAFCRRGPEGSTTRRRVFDARWHQGDGDVLHFEIRAKAFCWQMVRS
                     IVGTLVEAGSGKRRPGEVLAVLRSGDRAQAGQLAPPHGLCLWDVGYDD"
     gene            complement(3133..4644)
                     /locus_tag="VIJ44_00295"
     CDS             complement(3133..4644)
                     /locus_tag="VIJ44_00295"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY5164364.1"
                     /translation="MSVTASPTWLAPHHHEAADAALTVLTDVSLDRVVDMVLSASDGV
                     YEARSHEGAVQFRRGSTGQIEVVATEGRDPLTDQATDKFSSLAAERAQPHPDRSENAY
                     PHAFDQVAQLFDHPAAPDLCVIHSAAHNWEDQGGHRGEHGSLGIVQARAPFILAGKGV
                     RHDGIVERSARLVDVAPTILELLGVDAAAAGEHLAGQDGEVRRDALDVSGGRPGHVVA
                     FLFDGTNANVLHDMVARGEAPNVARLIDSGIAYGHGAMAGLPTVTLANHTSIMTGRLP
                     GHHGVLHNAWFDRRTGEQVITNSSSTWPWCMQHLFPGVDSVHSVLRRARPETFTASVD
                     EPCDHGASWSTFDAFRRGEVPTPSAKESLPHTTERFVRPHKDYRWSTIIDHAGVEQAV
                     GILGGQFRDETFPIPTFLFCNFTLTDAAMHAGGPYSEIAAAAVRDTDARMGEVIAAVE
                     SAGIFDDTAFVLVADHGMEENDPTATGDWDITLRDAGLRFRDEGYGFLYFDES"
     gene            4790..5248
                     /gene="rplM"
                     /locus_tag="VIJ44_00300"
     CDS             4790..5248
                     /gene="rplM"
                     /locus_tag="VIJ44_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017621569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEY5164365.1"
                     /translation="MRTFQPKPSDIERAWHVIDAEGAVLGRVASEVATLLRGKHKPIW
                     APQADVGDHVVIINAARLDIAIRKAQDKRYWRHSGYPGGIRSETLEHLLARDPIKVVR
                     LAVKGMLPKGPLGRQMIKKLRVYAGPTHPHAAQQPKPRALTSARRDAPAA"
     gene            5265..5657
                     /gene="rpsI"
                     /locus_tag="VIJ44_00305"
     CDS             5265..5657
                     /gene="rpsI"
                     /locus_tag="VIJ44_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEY5164366.1"
                     /translation="MPKPLIQTTGRRKEAVARVRLRPGSGKVIINGRPVEQYFTILTH
                     VQRATEALRLTQTADVYDVDATMHGGGVSGQAGALRLGIARALVALDDEVRPQLKKAG
                     LLVRDAREKERRKYGLKKARKAPQYSKR"
     gene            5689..7044
                     /gene="glmM"
                     /locus_tag="VIJ44_00310"
     CDS             5689..7044
                     /gene="glmM"
                     /locus_tag="VIJ44_00310"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003998794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEY5164367.1"
                     /translation="MTLRFGTDGIRGVANRELTPELVTALGRAAARVLGIDRPFVIGR
                     DTRRSGPLIEAALVAGICSEGADVELAGVVPTPTVAFLASSRSAPGAMISASHNSFED
                     NGVKLFAPGGQKMSDALEQRIEEELRELAVSSPGPGPGGSSVGVVRPMRDAIDEYIPH
                     VVGSLEGRSLESLTIVLDCANGAAFRTAPKIFRELGATVEIVNAAPDGTNINANSGST
                     DPSHLRQAVLDRGARAGLAFDGDADRVIAVDERGGLVDGDQIIAVCALDLAERDRLRN
                     RAIVATVMSNLGLRKCLETHGLGFVEVPVGDRHVIDEMERRDLVLGGEQSGHVIFRDH
                     ATTGDGVLTGAMLLDVVVGTGHSLSLLAGVMQRFPQILRNVPVRDRAALDEAAEFWEE
                     AASIERDLGPDGRVLVRPSGTEPVVRVMVEAPGLDQAELVADRLVSAVQRACGAPPEP
                     A"
     gene            7057..>8190
                     /locus_tag="VIJ44_00315"
     CDS             7057..>8190
                     /locus_tag="VIJ44_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate synthase"
                     /protein_id="HEY5164368.1"
                     /translation="MCGIIGVVRRRATREPPELSELISTLDEARAKLADWSGRIESLH
                     EAAAKLSRVDTDLRGAPGVFALVGDPAGAHELDVRSEAIDALVTMIEQRLDTGEQPAS
                     GVELEELNAALVALRDPAWTIRHDRIRTAGAVEDLAGSGASRAALESFGSVQLALSAI
                     DRLEVRGRDSAGLHLLVQGHGLDLTVPAVRARIDARAADPLFGSGSVRAFAETVVFVY
                     KASAEIGELGDNTRRLRAAIRGDELLHEALAVDTAQTQVLGHTRWASVGIISEPNAHP
                     LTGEEPHRDGPYVAAALNGDVDNYAELIVLDDLRIPAEITTDAKVIPTLMTHRRAAGS
                     DPIEAFRTTVTGLDGSVAIAAAQADVPDALLLALRGSGQALYVG"
BASE COUNT         1369 a         2834 c         2733 g         1254 t
ORIGIN      
        1 acggcgtcgc acacatcaaa tcgtcgttca acaacacgat catcacgatc acggaccagc
       61 aaggcaacgc gctcgcgtgg gcctcgtcag gcaacgtcgg cttcaaggga tcgcgcaaga
      121 gcacgccgtt cgcggcgcag ctggcggcag agaccgtcgc ccggcgcgcg atggagcacg
      181 gggtccgcaa ggtcgacgtg ctggtcaaag gccccggctc gggccgtgag accgccattc
      241 gaaccatcca gaacactggc atcgaggtgg tcggcatcaa ggacgtgaca ccgatccccc
      301 acaacggctg tcggccgccg aagcgccgga gggtctgacc catggcccgt tacaccgaga
      361 gcgtctgcaa gctctgtcgg cgtgagcgca tgaagctgtt cctcaagggg gccaagtgcg
      421 acaccatgaa atgcccggtg gagcgcaagc cgtacccgcc cggtgagcac ggccgcggtc
      481 gcatccgcga gagcgagtac ctgctccagc tccgcgagaa gcagaaagcc cggcgaatct
      541 acgggctcct cgaacgccag ttccggaacc tctaccagca ggcgtcgcgt gagcctggca
      601 tcaccggcga aaccctgctg cgcatgctcg agcagcgaat tgacaacgtc gttttccgca
      661 cgaacttcgc ggcgagccgc aaccaggccc gccaattcgt ccggcacgga cacgtgctcg
      721 tgaacggaaa gcgcgtcacg atcccctcgt accgagttcg gcccggcgac gcgatcgcca
      781 tcgggcccaa ggtcaaggac aacatcatca tccggcataa catcgacacg attgaccggg
      841 ctgtgcccgc gtggctcgac gtgagctcgg accggctcga agccacggtc cgagatgtac
      901 cgctccgcga gcagatcgac gtgccggtgc gcgaacaact catcgtcgag ctctactcca
      961 agtaagcccc ccagggcccg cggccgcggg cctatccgcg tttcggaaga agacagcaca
     1021 tgctcatcat ccaacgaccc gaagtcgagg ccggcgagcc cgacggcaac acacagtcgt
     1081 tcatcatctc gccgctcgaa ccggggttcg gtcacacgct cggcaacagc ctgcgtcgca
     1141 ccctgctttc gtcgatcccg ggtgctgctg tgacccaggt gcgcttcgac gaggcgctcc
     1201 acgagttcga cacgattccc ggcgtcaaag aggatgtcac cgacctgatc ctcaacatca
     1261 aagacctcgt gcttcgttgc tcgagtgccg atccggtgac cctccggttg gacaagcgtg
     1321 gtccggggga cgtcaccgcc ggtgacctcc aaaccacctc cgacgtggag gtgctcaact
     1381 cggagctgca tgtcgcgacg gtcaacgcca agggtcgtct cgccatcgat ctcaccgtcg
     1441 agcaagggcg cgggtacctg tccgccgaac gcaataagcg gacctccacc atcggtgtga
     1501 tcccggtcga cgcgatcttc tccccggtgc gccgcgtcgc gttctcgatc gagccgaccc
     1561 gggtcgagca agccaccaac tacgacaagt tgaccattga aatcgagacc gacgggtcga
     1621 tcaccccgcg ggaggcattg gcatccgcgg gggacacgct gcggagcctg gtcgggcttg
     1681 tcgctgatct gtcggacacg cctcagggtc tcgagctcgg agaggtgtcg ccggccaccg
     1741 cggcgtcccc cgacctcgag ctcccgatcg aggagctcga cctctccgaa cgtccccgca
     1801 actgcttgaa gcgggcccgg gtcgacacca tcggtcagct cgcgcagaag accgaggatg
     1861 atcttctggc gatcaccaac ttcgggtcga agtcgctcga ggaagtgctg cagaagctcg
     1921 acgagcgagg cctctcgttg cgggtgaagg agtagcaccg tgccgacgcc gacgaagggt
     1981 ccgcgcctcg ggcgggaccc ggcacaccag cgcctcctgc tcgccaacct ggcggcacac
     2041 ctcttcgagg cgggcgcgat cgtgacgacg gagacgaagg cgaaggccct tcggccctac
     2101 gcggagcatc tgatcacgaa ggctcggcgt ggaaccgtgc acgaccgccg gcaggtcgtc
     2161 gcgaagctgc acgacaaggc agttgcccac aaactcttcg ccgagatcgc tccccggtat
     2221 gaggagcgcc ccggtggcta cctgcggatc ctgaagctcg ggccgcgtcc gggtgacaac
     2281 gcgccgatgg cgcgtgtcga actggtgtga ccctcttcga cgccggcacg ccggcgtcgc
     2341 cgcaccggga aaccggttca cgcctcaaac tgctcatcgc ctacgacggc accgattttc
     2401 acggcttcgc ctcgcagcgc gtggttcgca ccgtcgaagg tgtgctcacc gacgccctgg
     2461 agaaggtgct gggccgcccg agcgcgtcac tggagctggt ctgtgccggg cgcaccgaca
     2521 agggtgtcca cgcgcggggc caggtcgtca gcgtggaggc ttcgcccggg actgactgcg
     2581 cggaggtcca tcaagcggtc aaccgcatga tcggtcccga ggtcgtcgtg cgctgtgtcg
     2641 agctagccga tgacgacttc gacgcacgac ggtctgctcg gtggcgtcgg taccggtaca
     2701 cgatcgtgaa cgggtccgaa cccgatccgt tcctggctcg tttcgcatgg tgggtgccgg
     2761 gcccacttga tcttcggatg ctgcgcctcg cggcggaccc gttcgttggc gatcacgatt
     2821 tcgccgcgtt ctgccgtcga gggccggaag ggtccacgac ccggcgacgg gtcttcgacg
     2881 cgcgatggca tcaaggcgac ggtgatgtcc ttcacttcga gattcgggcg aaggcgttct
     2941 gttggcagat ggtgcggtcg atcgtcggga ccctcgtcga agcggggtcc gggaagcgcc
     3001 gcccgggaga ggtgctcgcg gttctgcggt cgggtgatcg agctcaggcc ggtcagctcg
     3061 cgccgccgca cggcctgtgc ctctgggacg tcggatacga cgactgatca ggggccggtc
     3121 tcgacccttt gatcacgact cgtcgaagta gaggaagccg tagccctcgt cgcggaaccg
     3181 caacccggcg tcgcgcaacg tgatgtccca gtctccggtt gccgtcgggt cgttttcctc
     3241 catcccgtgg tcggcgacga ggacgaacgc agtgtcgtcg aagatcccgg ccgactcgac
     3301 ggcggctatg acctccccca tgcgggcgtc ggtgtcgcgc acggcggccg ccgcgatctc
     3361 ggagtaggga cccccggcgt gcatcgcggc gtcggtcaac gtgaagttgc agaacaagaa
     3421 cgtcgggatc gggaacgtct cgtcgcggaa ctggccgccg agaatcccga ccgcttgctc
     3481 gacaccggcg tggtcgatga tcgtggacca ccggtagtcc ttgtggggcc gcacgaagcg
     3541 ctcggtggtg tgggggagtg actccttggc gctcggggtc ggcacctcac cacgccggaa
     3601 ggcgtcgaac gtcgaccatg acgctccgtg atcacagggc tcgtcgacgg acgcggtgaa
     3661 ggtctcgggg cgagcccgtc gcagcacgct gtggactgag tcgacccctg ggaataggtg
     3721 ctgcatgcac catggccatg tcgacgacga gttcgtgatc acctgctcgc cggtgcgtcg
     3781 gtcgaaccac gcgttgtgca gcacaccgtg gtgaccggga agtcggcccg tcatgatcga
     3841 cgtgtggttg gccaacgtga cggtcggtag accggccatc gcaccgtgac cgtacgcgat
     3901 cccagaatcg atcagccggg cgacgtttgg agcctcacca cgggccacca tgtcgtggag
     3961 cacgttcgcg ttggtcccgt cgaacaggaa ggcgacgaca tggcccggcc ggccgccgct
     4021 cacgtctagc gcgtcgcgac ggacctcgcc gtcctgaccc gcgaggtgct cgccggccgc
     4081 ggccgcgtcc acgccgagca gctcgaggat cgtcggggcc acgtcgacga gtcgagccga
     4141 gcgctcgacg atgccgtcgt ggcgaacgcc ctttccggcc aggatgaacg gagcgcgggc
     4201 ctgcacgatc ccgagggagc cgtgctcacc gcggtgcccg ccctggtcct cccagttgtg
     4261 ggcggccgag tggatcacgc agaggtccgg tgcggccgga tgatcgaaaa gctgcgccac
     4321 ctggtcgaag gcgtgcgggt aggcgttctc gctgcggtcg ggatggggct gggcccgctc
     4381 ggcggcgagc gacgagaatt tgtcggtggc ctggtccgtc agaggatcgc ggccctcggt
     4441 ggccacgacc tcgatctggc cggtgcttcc ccggcggaac tgcaccgccc cttcgtgcga
     4501 tcgcgcctcg tagacaccgt ccgacgcgct gaggaccatg tccacgaccc gatcgaggga
     4561 cacgtcggtc aggaccgtga gggcggcgtc agcggcttcg tggtggtgcg gggccagcca
     4621 cgtgggcgaa gcggtgacgc tcacagcggc gagggtactg ggtcgaggcg ggacggattg
     4681 ccccatcccc gcccccgtcg gtaccatgag acgctgcgtt cacggccggg tcagctgccc
     4741 ggctgccgac cgccgtgaac ccgagccccg ctcacgaacc cgggacgctg tgcgcacctt
     4801 ccaacccaag ccttcggaca ttgaacgcgc ctggcatgtc atcgacgccg aaggtgccgt
     4861 gctcggccgc gtcgcgtccg aggtcgcgac cctcctccga ggcaagcaca agccgatctg
     4921 ggcgccgcag gccgatgtcg gggatcacgt tgtcatcatc aatgccgcgc gtctcgacat
     4981 cgcgatccgc aaagcccagg acaagcggta ctggcggcac tcggggtatc ccggcggcat
     5041 ccgcagcgag accctcgagc acctcctggc ccgcgacccg atcaaagtgg tgcgcctcgc
     5101 ggtgaagggc atgctcccga agggcccact cgggcgccag atgatcaaga agctgcgcgt
     5161 ctacgccggc ccgacccacc cgcacgccgc gcagcaaccg aagcctcggg ccctcacctc
     5221 ggcgcgtcgc gacgcgccag cggcctaaac cgacaaggga cacgatgccc aaacctctca
     5281 tccagaccac cggccgacgc aaggaagccg tcgcccgcgt ccgactccgt cccgggtcgg
     5341 gcaaggtgat catcaacggc cgcccggtcg agcagtactt cacgatcctg acgcacgtgc
     5401 agcgcgccac cgaagccctc cgcctcactc agaccgccga cgtctacgat gtcgatgcca
     5461 cgatgcacgg cggtggagtc agcggccagg ccggcgcact gcggctcggg attgcccgag
     5521 cactcgtcgc tctcgacgac gaagtgcgcc cgcaactcaa gaaagccgga ttgctcgtgc
     5581 gcgacgcgcg cgagaaggaa cggcgcaagt acggcctcaa gaaggcccgc aaggcgcccc
     5641 agtactcgaa gcgctagcgc gcccccgcgc gccgcctcag cgcgacccgt gactctccga
     5701 ttcggaaccg acggcatccg tggtgttgcc aaccgcgagc tgacgccgga gctggtgacc
     5761 gccctcgggc gagccgcggc ccgagtgctc ggcattgacc gaccgttcgt catcggacgc
     5821 gacacacgac ggtccggacc gctgatcgaa gccgcgctcg tggccgggat ctgctcggaa
     5881 ggcgccgatg tcgagctggc cggcgttgtt ccgaccccta ccgtcgcctt cctcgcgtcg
     5941 agtcgcagcg cacccggcgc catgatctcg gcgagccata acagcttcga agacaacggg
     6001 gtcaagctgt tcgccccggg cggccaaaag atgagcgacg cgctcgaaca gcgaatcgag
     6061 gaggaactgc gggagctcgc ggtcagctcg ccaggaccgg gaccgggtgg cagctccgtc
     6121 ggggtggtcc gccccatgcg tgacgcgatc gacgagtaca tcccccacgt cgtcgggtcc
     6181 ctggaaggac gcagcctgga gtccctgacc atcgtgctcg actgcgcaaa cggcgcggca
     6241 ttccgcaccg caccaaagat cttccgcgag ctcggtgcca cggtcgagat cgtcaacgcc
     6301 gcacccgacg gcaccaacat caacgccaac tccggatcga ccgatccgtc gcacctccgg
     6361 caggcggtgc tcgaccgtgg cgcccgggct ggtctcgcat tcgacgggga tgccgatcgc
     6421 gtcatcgccg tggatgaacg aggcgggctg gtcgacggcg accagatcat cgcggtctgc
     6481 gccctggacc tcgccgaacg cgaccggcta cggaaccggg ccatcgtcgc caccgtgatg
     6541 tcgaacctcg ggctccggaa gtgtctcgag acccacggcc tcggcttcgt cgaggttccg
     6601 gtcggggacc gtcatgtcat cgacgagatg gaacgtcgcg acctcgtgct cgggggcgaa
     6661 cagtccggcc acgtcatctt ccgggatcac gccacgaccg gcgacggcgt cctgaccggc
     6721 gcgatgctgc tcgatgtcgt ggtcgggacc ggacactccc tttcgctcct cgccggcgtc
     6781 atgcagcgct tccctcagat cctgcggaat gtccccgtcc gggaccgcgc cgcgctcgac
     6841 gaagccgcag agttctggga agaggcggcg tccatcgagc gcgatcttgg acccgacggg
     6901 cgcgtcctcg tccggccctc cggtaccgag cctgtggtcc gcgtgatggt cgaggcaccc
     6961 ggcctcgacc aggccgagct ggtcgccgac cgtctcgtca gcgccgttca gcgggcctgc
     7021 ggtgctccgc cggagcccgc gtaggcttga ccgtcgatgt gcggaattat cggtgtggtt
     7081 cgccgtcgag cgacgcgcga gccaccggag ctctccgagc tgatctcgac cctggacgag
     7141 gcgcgagcaa agctggccga ctggtcgggg cgaatcgaat cgctccatga ggccgccgcc
     7201 aagctgagcc gcgtcgatac cgacctgcga ggcgcgcccg gggtgttcgc gctcgtgggc
     7261 gaccccgccg gtgcgcacga gctcgacgtt cggagcgagg ccatcgacgc gctcgtgacc
     7321 atgatcgagc agcgtctcga caccggcgag cagccggcca gcggcgttga gctcgaagag
     7381 ctcaacgcgg cgctggtcgc gttgcgggac ccggcgtgga cgatccgcca tgaccgcatc
     7441 cgaacggcgg gcgcggtcga agaccttgcc ggatccggcg cctcacgggc agcgctggag
     7501 tccttcgggt cggttcagct ggccttgtcg gcgatcgatc ggctcgaggt tcgtggacgc
     7561 gactccgccg gtctgcacct gctcgtccag gggcacggtc tggacctcac cgttcccgcg
     7621 gtccgtgccc gtatcgacgc tcgcgcggcc gacccgctgt tcggttccgg ctcggtgcgg
     7681 gcgttcgctg agaccgtggt gttcgtctac aaggcatccg ccgagatcgg agagctcgga
     7741 gacaacaccc gccggctccg ggccgcgatc cgaggtgacg agctgctcca cgaggcgctc
     7801 gcggtcgaca ccgcccagac ccaggtgttg ggccacactc gctgggcgag cgtcgggatc
     7861 atctcggaac ccaacgccca tccgctcacc ggtgaagagc cacaccggga cggcccctac
     7921 gtcgcggcgg cactgaacgg cgacgttgac aactacgccg agctcatcgt cctcgacgat
     7981 ctccgcatcc cggcagagat caccaccgac gccaaggtca tccccacgct catgacccac
     8041 cggcgcgccg cagggtcgga tccgatcgag gccttccgga ccacggtgac ggggttggac
     8101 ggctcggtgg cgatcgccgc ggcgcaggcc gatgtccccg acgccttgct cctggcgctc
     8161 cgagggagcg gacaggcgct ctacgtcggg
//
LOCUS       DATCCH010000014         2552 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_541509, whole genome shotgun sequence.
ACCESSION   DATCCH010000014 DATCCH010000000
VERSION     DATCCH010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541509"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="VIJ44_00320"
     CDS             <1..338
                     /locus_tag="VIJ44_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164369.1"
                     /translation="IRDLHGLDFDTRDGLDLSYAHYEHNDLQIHVAVGYLPMGDLPTA
                     SRVIARHLAENARDRDVVLDLVMWSPGPRPEVEVTAKAAADLLAECDFGRPLHRIDLT
                     ITWLEGGGP"
     gene            357..>2552
                     /locus_tag="VIJ44_00325"
     CDS             357..>2552
                     /locus_tag="VIJ44_00325"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEY5164370.1"
                     /translation="MTFRSDPAGSDFVEDTIYRNLHPMLGKRLDLWRLSNFELTRLPS
                     PEDVYVFDGVAKDNPRDHRLFALAEVRDLTAVHDPDTGRVTYPRLGRSGLAALAALRS
                     AMARYPARERPTANRLVVWVRPPWNIPRDEWHDLAMTYEGLARGASLEKVVLHVNIPD
                     TDDLGQRVLTDRIVVLEGLGAGGPTIRMQEPGPNPVRPLTPYAQKVLTAARFGSPYPY
                     EIVRMLTPAEGDASPFPPGSFQELDLGADDDLVPVDRLPGGNTAHLVVGLLTSRTGVH
                     PDGLTRVALLADPTQGLGNLAEPECRRVNAALALALDRGIPVEWYAVSSGALIALDSG
                     TENMDWIALTLRRLIEYTQAGGEVNIVITGITVGGQPYWNAEATMLMHTKGILVMTPA
                     STMVLTGKQALDFSGAVSADDNQGIGGFDRIMGPNGQAQYWAPSFPEACALLLRHYDF
                     TYAVPGERFPRRRPTNDPVDRDVRTSPHARLPESGFTTVGDVFDAATNPERKLPFDMR
                     SVMRAVADQDVEPLERWRQWQDADTAIVWDATVGGIPVCMIGIESHRVRRAGFVPSYG
                     PPSWTSGTLFPQSSRKIARAVNAASGNRPLVVLANLSGFDGSPESMRQWQLEYGAEIG
                     RGVTNFEGPIVFVVVSRYHGGAFVVFSKALTETMEIAAVEGSFASVIGGAPAAATVFA
                     REVKRRVERDPRVESARAEAASAGGPQAAALRARAARVVEEVRSQKLHDV"
BASE COUNT          381 a          948 c          860 g          363 t
ORIGIN      
        1 gaatccggga tctgcacggg ctggacttcg acacccgcga cgggctggac ctcagctacg
       61 cccactacga gcacaacgac ctgcagatcc atgtcgccgt cggctaccta ccgatgggcg
      121 acctcccgac ggcatcccgc gtcatcgctc ggcacctcgc cgagaacgcc cgcgaccggg
      181 acgtcgtcct tgacctcgtg atgtggagtc cgggtccccg cccagaggtg gaggtgactg
      241 ccaaggcggc cgccgacctg ctcgccgagt gcgacttcgg tcggcccctg caccggatcg
      301 acctcaccat cacctggctc gagggcggtg ggccgtagcg gagccgcacc cagcacgtga
      361 ccttccgctc ggatccagcc ggttccgact tcgtggagga cacgatctac cgcaacctcc
      421 acccgatgct gggcaagcgc ctcgacctgt ggcggctgtc gaacttcgag ctcacccggc
      481 tcccctcccc cgaggacgtc tacgtcttcg acggcgttgc caaggacaac cctcgagacc
      541 accggctctt cgccctcgcc gaggtccgcg acctcaccgc cgtccacgac ccggacaccg
      601 gccgcgtcac ctatccgcgg ctcgggcgca gcggcctcgc agccctggca gccctgcgca
      661 gtgccatggc ccgctacccc gcccgcgagc ggccgaccgc gaaccggctc gtcgtctggg
      721 tgcgaccccc gtggaacatc ccccgggacg agtggcacga cctcgcgatg acctacgagg
      781 gcctggcccg cggcgccagc ctggagaagg tcgtcctgca cgtcaacatc ccggacaccg
      841 acgacctggg ccagcgggtg ctcacggacc ggatcgtcgt cctcgaaggg ctcggcgccg
      901 gtggaccgac gatccggatg caggagcccg ggccgaaccc cgtccgaccc ttgacgccgt
      961 acgcgcagaa ggtgctcacc gcggcccgct tcggcagccc ctacccctac gagatcgtcc
     1021 ggatgctcac tccggccgag ggcgacgcct cgcccttccc gccgggcagc ttccaggagc
     1081 tcgacctcgg cgccgacgac gacctcgtgc cggtcgaccg gttgccgggg ggcaacacgg
     1141 cgcacctcgt cgtcgggctg ctcaccagtc gcaccggcgt gcaccccgac ggcctgaccc
     1201 gggtggccct cctggcggac cccacccaag gcctgggcaa cctggccgag cccgagtgcc
     1261 ggcgggtcaa tgccgcgctc gccctcgcgc tcgaccgcgg catcccggtg gagtggtacg
     1321 ccgtgtcgtc cggcgcgctc atcgccctgg actccggcac ggagaacatg gactggatcg
     1381 cgctgaccct gcgccggctc atcgagtaca cgcaggcggg cggcgaggtg aacatcgtca
     1441 tcacggggat caccgtcggc ggacagccgt actggaacgc ggaggcgacg atgctcatgc
     1501 acaccaaggg catcctggtc atgaccccgg cgagcacgat ggtcctgacc ggcaagcagg
     1561 ccctcgactt ctccggtgcg gtatcggcgg acgacaacca gggcatcggc ggcttcgacc
     1621 ggatcatggg gccgaacggg caggcccagt actgggcgcc cagcttcccg gaggcgtgcg
     1681 cgctgctgct tcggcactac gacttcacct atgccgtgcc gggcgagcgc ttcccgcggc
     1741 gtcggccgac gaacgacccg gtggatcgcg acgtgcgcac ctccccgcac gcgcgcctgc
     1801 ccgagtcggg gttcacgacg gtgggcgacg tcttcgacgc cgccaccaac cccgagcgca
     1861 agctgccgtt cgacatgcgg tcggtgatgc gcgccgtcgc cgaccaggac gtcgaacccc
     1921 tcgagcgatg gcgccagtgg caggacgccg acacggcgat cgtgtgggac gccaccgtcg
     1981 gcggcatccc ggtgtgcatg atcggcatcg agtcacacag ggttcggcgc gcggggttcg
     2041 tcccctccta cggccccccc tcatggacgt cgggcacgct cttcccacag tcctcgcgca
     2101 agatcgccag ggcggtcaac gcggcgagcg ggaaccgccc gctggtcgtg ctcgccaacc
     2161 tgtcgggttt cgacgggtcg ccggagtcga tgcggcaatg gcagctcgag tacggcgccg
     2221 agatcggccg tggggtgacg aacttcgagg gtcccatcgt cttcgtcgtc gtctcgcggt
     2281 accacggcgg tgccttcgtg gtgttctcaa aggccctcac cgagacgatg gagatcgctg
     2341 cggtggaggg gtccttcgcc tcggtgatcg gcggtgcccc ggccgccgcg accgtgttcg
     2401 cccgtgaggt caagcgtcgg gtcgagcgcg acccgagggt cgagagcgcc cgagcggagg
     2461 cggcgtcggc aggcggtcct caggcggccg cgctcagggc gagagcggcc agggtcgtgg
     2521 aggaggtgcg ctcgcaaaag ctacacgacg tc
//
LOCUS       DATCCH010000015         8378 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_168291, whole genome shotgun sequence.
ACCESSION   DATCCH010000015 DATCCH010000000
VERSION     DATCCH010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 8378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8378
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168291"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1193)
                     /locus_tag="VIJ44_00330"
     CDS             complement(<1..1193)
                     /locus_tag="VIJ44_00330"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY5164371.1"
                     /translation="MPDWNLATVFESVCDALPDQTVIVQGERRHTWQSFDERAARFAG
                     ALAAYGVEPGSKVALYLYNSNEFLEAMYGSLKARAVHVNVNYRYLEDELLYLLENADA
                     EVLVFHGVLAEHVGAVRDRLPKLRLVVQVDDGSPLLDGAVPFEKLLAAHDPAPRIERS
                     GDDLWFLYTGGTTGMPKGVMWRNEDFYGSLGENTYTLFGETLPTTSDEAGAAAARIVA
                     TGASPTHLPASPLMHGTGVMTTIQSLFLGGSVVTLENRHFDADELWQTVARERVSQLA
                     IVGDAFAKPMVRSLEDAEARGAPYDLSSLRLIVSSGVIWSAEVKAELQARQPMILLDS
                     LGSSEGIGFASSITAPGDEARTARFRVGPRTKVLTDDGREVASGSGEQGMLALGGHLP
                     AGYYK"
     gene            1237..1932
                     /locus_tag="VIJ44_00335"
     CDS             1237..1932
                     /locus_tag="VIJ44_00335"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEY5164372.1"
                     /translation="MASRGGQQRIPRPPSVRAGAPAPWAHLRPAERRFTLAEVRRAAE
                     QLPEPHRPPPPPTARPAAVLVPIFETGGEARIILTKRPETMPSHRGEIAFPGGKLEAE
                     RDGSLRDAALREAHEEIGLDPHDVDVVAELDSLTTVASRFVLTPFVGLLDTRPQLHPD
                     PSEVVSVFDVAISELLRDDVYREERWDIPPDLGITPGRDRAIHFFELSDETVWGATAR
                     ILAGLLAHFTAGR"
     gene            2103..2849
                     /locus_tag="VIJ44_00340"
     CDS             2103..2849
                     /locus_tag="VIJ44_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEY5164373.1"
                     /translation="MAIVAGALGLTACFAPVAPPPVGAGTIMGPSVLTAAQIAAYVCH
                     VGRCATWLPEISAQQMAQLYIDEGNLVGVRGDIAFSQAVLETGWFAFLGSPAQNLVPP
                     PAPGDTTWPGFVLPRDHNYAGLGAFPGSTVFMRMPTPQDGVRAQLQHLRNYADAGSRH
                     DNLGAPFEARPGYDAYAFDHFFYKGQAPRWVDLDGRWAVPGTTYGQTILSIDNNMRTY
                     AGLAPIASSGLSASSLLTTPALHATTTDRH"
     gene            2947..4125
                     /locus_tag="VIJ44_00345"
     CDS             2947..4125
                     /locus_tag="VIJ44_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HEY5164374.1"
                     /translation="MRSDVEVEIGIGKAARRAYGFDEIAIVPSRRTRDPEDVDISWEI
                     DAYPFELPLIAAAMDGVASPRTAIDIGHLGGLACLNLEGLWTRYEDPEPLFEEIAELP
                     AEKATRRMQEIYAEPIRDDLIGARIREIKEGGVTACASLTPQRVEQYAKHVLDAELDL
                     LVIQGTVVSAEHVSKDESRQPLNLKKFIREFDLPVIVGGCASYSTALHLMRTGAVGVL
                     VGVGPGHACTSRGVLGIGVPQATAIADAAGARIRHLDETGVYVHVIADGGMRTGGDVA
                     KAIACGADAVMIGSPLAQAYEAPGRGYHWGMATFHPTLPRGARVYAGHNASLAEILVG
                     PAHENDGTLNLFGALRTSMATTGYQTLKEFQKAEVMVAPALKTEGKVLQREQHIGMGR
                     "
     gene            4097..5677
                     /gene="guaA"
                     /locus_tag="VIJ44_00350"
     CDS             4097..5677
                     /gene="guaA"
                     /locus_tag="VIJ44_00350"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013427280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEY5164375.1"
                     /translation="MSSTSAWAAEPRPLTPQHDTVLVVDFGAQYAQLIARRVREARVY
                     SEIVPRTMPLEEMLAKRPRGIIFSGGPASVHVDGAPSIDADIYRAGVPILGICYGAQL
                     AASQLGGDVRRTGGGEYGRTPMTRTDAGSLLLTDVAGEQTVWMSHGDAIGAPPEGAVV
                     TATTPGAPVAAFEDRDRALFGVQFHPEVLHTERGREVLHAFLFDVCGARPTWTHTSII
                     DEAVATVREQVGDARVVCGLSGGVDSAVAAALVSKAVGDQLTCVFVDTGLLRLGEADQ
                     VEETFRRQFQVDLVHVKAADRFLAALTGVTDPEQKRKMIGTTFIRVFEEVAAELSDAG
                     YLVQGTLYPDIVESGGPDNATIKSHHNVGGLPDDMRFELVEPLRNLFKDEVRAVGEEL
                     GLPDEIVWRQPFPGPGLAVRIIGEVTPERLETLRRADHVVTEEVRRAGLYRELWQSFA
                     VLPAVRTVGVMGDDRTYEYPIVVRAVTSEDAMTADWARLPHDVLERIASRIINEVPGV
                     NRVAYDITSKPPGTIEWE"
     gene            5683..6708
                     /locus_tag="VIJ44_00355"
     CDS             5683..6708
                     /locus_tag="VIJ44_00355"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HEY5164376.1"
                     /translation="MNTNTLEATARALVAPGKGILAADESTGTIKKRFDTIGVENTEE
                     NRRAYRDLLFTTDGIANHISGVILYDETIRQSSLDGTPFPKLLERQGVISGIKVDTGA
                     KPLALADDGETITEGLDGLRDRLAEYHGLGARFAKWRATYTISDNLPSQYAIDANADA
                     LARYAALCQEIGIVPIVEPEVMMEGDHTIQRAAAVSSATLEALYHALYEQRIHLPGSL
                     LKPNMVLSGYDAPVHASVDEVADRTLECFAARVPAAVPGIVFLSGGQSDEDATAHLDA
                     MNKRGPHPWELSFSYGRALQAAPLHAWRGDPANRTSAQAAFLLRAKLNSLARSGKYSP
                     DMEQQAA"
     gene            6730..7167
                     /gene="dtd"
                     /locus_tag="VIJ44_00360"
     CDS             6730..7167
                     /gene="dtd"
                     /locus_tag="VIJ44_00360"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014942190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEY5164377.1"
                     /translation="MRALVQRVTEARVTIDGAVVGEIGVGLCVFAGVTHGDGEPQARR
                     LAEKLWQLRVFPDDDGVMNRSLAETTGAALVVSQFTLYADTRRGRRPSWAAAAPPARA
                     APLVASLTHALRELGATVATGRFGADMSVELVNDGPVTLLLEV"
     gene            7347..>8378
                     /locus_tag="VIJ44_00365"
     CDS             7347..>8378
                     /locus_tag="VIJ44_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEY5164378.1"
                     /translation="MTDVPTASAPSVGERDDIERLLGGLNPVQREAAAAPDGPLLIVA
                     GAGSGKTRVLTHRIAYLIATGRVSPFNLLAITFTNKAAGEMKQRVATLVGPVAHRMWV
                     STFHSACARILRREAARVGLRSSFTIYDQADAVRLTDYVRRDLNLDPKRFPPRQLHAR
                     ILALKNDLVGPDEALAAAVTPPERRYAEIYVEYQRRLLEASAVDFDDLLLLAVRLFRD
                     HPDALTRWQHRFAHVLVDEFQDTNAAQWELVRLLTAGSRNVMVVGDHDQAVYGFRGAD
                     YRNLVRFEETFPDATVIVLEQNYRSTQAILDAANAVIANNAARQPKHLWTEQIGGELL
                     TQYQAEDEHD"
BASE COUNT         1328 a         3141 c         2650 g         1259 t
ORIGIN      
        1 tccttgtagt agccagccgg caaatgcccg ccgagcgcca acatgccctg ctcgcccgag
       61 ccggaggcca cctcgcgccc gtcatcggtc agcaccttgg tgcgcggccc gacccggaac
      121 cgggcggtgc gggcctcgtc tcccggcgcg gtgatgctgc tggcaaagcc gatcccctca
      181 ctcgagccca gactgtcgag cagaatcatc ggctgacgag cttggagctc tgctttgacc
      241 tcggcgctcc agatcacccc gctgctgacg atcaaccgga gcgacgagag gtcgtagggc
      301 gcaccgcggg cttcggcgtc ttcgagcgag cgcaccatcg gcttcgcgaa ggcgtcgccg
      361 acgatcgcca gctgcgacac cctctcccgg gcgacggtct gccagagctc gtcggcgtcg
      421 aaatgacggt tctcgagggt gacgacgctg ccgccgagga acagcgattg gatggtggtc
      481 atcacgcccg tcccgtgcat caacggcgag gccgggagat gtgtgggcga cgccccggtc
      541 gcgacgattc gggccgcggc ggcgcccgcc tcgtcggagg tggtcggcag cgtctccccg
      601 aacagcgtgt aggtgttctc gcccaacgag ccgtagaagt cctcgttgcg ccacatcaca
      661 cccttcggca tgccggtggt cccgcccgtg tacaagaacc agaggtcgtc acccgaccgt
      721 tcaatgcggg gcgctgggtc gtgggcggca aggagcttct cgaagggcac cgccccgtcg
      781 agcaacgggc tgccgtcgtc gacctgtacc accagccgca gcttcgggag ccggtcgcgc
      841 accgcgccga cgtgttccgc caacaccccg tggaagacca gcacttcggc gtcggcgttc
      901 tcgagcagat acaagagctc gtcctcgagg tagcggtagt tcacgttcac gtgcaccgcc
      961 cgggccttca acgacccgta catggcctcg aggaactcgt tgctgttgta caggtacaac
     1021 gccaccttcg agcccggctc gacgccgtag gccgcgagcg cgcccgcgaa tcgtgccgcc
     1081 cgctcgtcga acgactgcca cgtgtgtcgc cgttcgccct gcacgatcac ggtctggtcc
     1141 ggcaaggcgt cgcagaccga ctcgaagacg gtggccaggt tccagtcggg catagcgctc
     1201 ccggatcgat gtcggcggga gcatacgctt cgcttcgtgg cgagtcgtgg gggccagcag
     1261 cgcatccccc ggccaccctc ggtgcgcgcc ggtgccccgg ccccgtgggc gcacctgcgc
     1321 ccggccgagc ggcgcttcac cctcgccgag gtccgccggg ccgccgagca gctacccgaa
     1381 ccgcaccgtc cgccgccgcc gcccaccgcc cgtcccgccg cggtgctggt cccgatcttc
     1441 gagacgggcg gcgaggcgag gatcatcctc accaaacggc ccgagaccat gccgtcgcac
     1501 cgcggcgaga tcgcctttcc cggtgggaag ctcgaagcag agcgggacgg ctcgctgcgc
     1561 gacgccgcgt tgcgcgaggc gcacgaagag atcggtctcg atccccacga cgtcgacgtc
     1621 gttgccgaac tcgacagtct caccacggtc gcgtcccggt tcgtgctcac tccgttcgtc
     1681 gggctgctcg acacccggcc ccagctccac cccgaccctt cggaggtggt gagcgtcttc
     1741 gatgtcgcga tctcggagct gctccgcgac gacgtctacc gcgaggaacg ctgggacatc
     1801 cctcccgacc tcgggatcac ccccggacgg gatcgggcca tccacttctt cgagctcagc
     1861 gacgagacgg tttggggggc gacggcccgg atcctcgccg gcctcctcgc gcacttcacc
     1921 gccgggcgct gacccgcccc cggtttgggg ctccagtcgg ccccacccct ggtcgacatt
     1981 ctcccgaggg ccactcgggc cctggaggcg cgcgcatgca gatccgaact cgcgacgacg
     2041 ctgctcctcg ccgcgccccg acgtgccgtc cgcaccgacg ccgtggccgg cgaagcgcgg
     2101 cggtggcgat cgtcgcgggc gccctcgggc tcacggcgtg cttcgcgccc gtcgccccgc
     2161 caccggtcgg tgccgggacc atcatgggtc ccagcgtgct caccgcggcc cagatcgcgg
     2221 cgtacgtctg tcacgtcggt cgctgcgcca cttggctgcc cgagatctcc gcgcagcaga
     2281 tggcccagct gtacatcgac gaaggcaacc tggtcggcgt acgcggtgac atcgccttca
     2341 gccaggccgt cctcgagacc ggctggttcg cgttcctcgg ctccccggcc cagaacctgg
     2401 tcccgccgcc cgcccccggt gacaccacct ggcccggttt cgttctgcct cgcgatcaca
     2461 actacgccgg cctcggcgcg ttcccgggca gcacggtctt catgcggatg cccacccccc
     2521 aagacggggt ccgcgcccag ctccagcatt tgcggaacta cgccgacgcc ggctcccggc
     2581 acgacaatct tggcgccccg ttcgaggccc gcccgggcta tgacgcctac gcgttcgacc
     2641 acttcttcta caagggtcag gcaccccggt gggtggacct cgacggccgc tgggccgtcc
     2701 ccggcaccac ctacggccag accatcctga gtatcgacaa caacatgcgc acctacgccg
     2761 gcctggcgcc gatcgcgtcg tctggtctga gcgcctcgtc gctgctcacc acccccgcgc
     2821 tgcacgccac caccaccgac cgccactgag accgacggtc gtcccaggcc tgtcgggtcc
     2881 tcaccgaagg cggtcgctgg tcccgccgca gggtggcggt accatcaccg ttctggcgaa
     2941 acgggcatga ggagcgacgt ggaagtcgag atcggtatcg gcaaagcagc ccgacgggcc
     3001 tacggcttcg acgagatcgc catcgtgccg agccgccgga cccgagatcc cgaagacgtc
     3061 gacatctctt gggagatcga cgcctatccg ttcgagcttc ccctcatcgc cgcggcgatg
     3121 gacggtgtcg cgtcaccccg caccgcgatc gacatcggtc acctcggagg tctggcctgc
     3181 ctcaacctcg aaggtctctg gacccgctac gaggaccccg agccgctctt cgaggagatc
     3241 gccgagcttc ccgccgagaa ggccacccgg cgcatgcagg agatctacgc cgagccgatc
     3301 cgagacgact tgatcggggc ccgcatccgc gagatcaaag aaggtggtgt gaccgcgtgc
     3361 gcgtcgctca ccccccaacg ggtggagcag tacgccaagc acgtcctcga cgccgagctc
     3421 gacctgctcg tcatccaggg caccgtggtg tccgccgagc acgtctccaa ggacgaatct
     3481 cgccaacccc tcaacctgaa gaagttcatc cgcgagttcg acctccccgt catcgtcggc
     3541 ggctgcgctt cgtactcgac ggcgttgcac ctcatgcgca ccggcgcggt cggtgtcctc
     3601 gtcggcgtcg gacccggcca tgcctgcacc agccgtgggg tcctcggaat cggcgtcccg
     3661 caggccaccg cgatcgccga cgccgccggc gcccggatcc gtcatctcga cgagaccggc
     3721 gtctacgtgc atgtgatcgc cgacgggggg atgcgcaccg gcggcgacgt cgccaaagcc
     3781 atcgcctgcg gagccgacgc ggtgatgatc ggctcacccc tcgcccaggc gtacgaagcc
     3841 cccggccggg gctaccactg gggcatggcg acgttccatc cgacgctgcc acgcggggcc
     3901 cgcgtctatg ccggacacaa cgcctccctc gccgagatcc tggtcgggcc ggcccacgag
     3961 aacgacggca ccctgaacct gttcggcgcg ctgcgcacgt cgatggcgac gaccgggtat
     4021 cagaccctca aggagttcca gaaggccgag gtcatggtcg cacccgcgct caagaccgaa
     4081 ggcaaggtgc tccaacgtga gcagcacatc ggcatgggcc gctgagccca ggccgttgac
     4141 gcctcagcac gacaccgttc tcgttgtcga cttcggcgcc caatacgccc agctgatcgc
     4201 ccgtcgcgtc cgcgaggcgc gcgtgtactc cgagatcgtg cctcgcacca tgcctctcga
     4261 ggagatgctg gccaaacgac cccgggggat catcttctcc ggtggtcccg cgtcggtcca
     4321 tgtcgacggt gccccgtcga tcgacgccga catctaccgc gccggggtac cgattctcgg
     4381 catctgctac ggcgcgcagc tcgcggcatc gcaactcggt ggggacgtgc gccgcaccgg
     4441 cggcggcgaa tacgggcgca ccccgatgac ccggaccgac gccgggtcgc tgctcctcac
     4501 cgacgtcgcc ggtgagcaga ccgtctggat gagccacggc gacgcgatcg gtgccccccc
     4561 ggagggggcg gtcgtgaccg ccacgacgcc gggcgccccg gtggcggcct tcgaggaccg
     4621 ggaccgggcc ctgttcggcg tccagttcca ccccgaggtg ctccacaccg aacggggccg
     4681 cgaggtgctc cacgccttct tgttcgacgt gtgcggcgct cgaccgacgt ggacccacac
     4741 gtcaatcatc gacgaagccg tcgcgacggt gcgcgaacag gtcggcgacg cccgcgtcgt
     4801 ctgtgggctc tccggcggtg tcgactccgc cgtcgcagcg gcgctcgtga gcaaagccgt
     4861 cggtgaccag ctgacttgtg tcttcgtcga caccggtctc ctccgtctcg gcgaagccga
     4921 ccaggtcgag gagacgttcc gacgccagtt tcaggtcgac ctcgtccacg tcaaggccgc
     4981 cgaccggttc ctggccgcgc tcaccggcgt gaccgatcct gaacagaaac gcaagatgat
     5041 cggaacgacg ttcatccgtg tcttcgaaga ggtcgcggcc gagctctccg acgccggata
     5101 cctcgtccaa ggcaccctct accccgacat cgtcgaatcg ggtggtccgg acaacgccac
     5161 catcaagtcc caccacaacg tcggcggact ccccgacgac atgcgcttcg agttggtcga
     5221 gccgctgcgc aacttgttca aggacgaggt ccgagcggtc ggcgaagagc tcgggctccc
     5281 ggacgagatc gtctggcgtc aaccgttccc cggtcctggc ctcgcggtcc gcatcatcgg
     5341 ggaggtcacc cccgagcgcc tcgagacgct gcgccgagcc gatcacgtcg tcacggagga
     5401 agtccgccgc gccgggctgt atcgtgagct ctggcagagc ttcgcggtgc tgccggcggt
     5461 gcgcaccgtc ggtgtcatgg gcgacgatcg cacctatgaa tacccgatcg tggtgcgggc
     5521 cgtgacgtcg gaggacgcga tgaccgcaga ctgggctcgc ctcccgcacg acgtgctgga
     5581 acgcatcgcc tcccggatca tcaacgaggt ccccggcgtc aaccgggtcg cctacgacat
     5641 caccagcaag ccacccggca ccatcgagtg ggagtgaacc cgatgaacac caacaccctc
     5701 gaagcgactg cacgagcgct cgtggcgccg ggcaaaggca tcctcgccgc cgacgagagc
     5761 accggcacca tcaagaagcg cttcgacacg atcggtgtcg aaaacaccga agagaaccgg
     5821 cgcgcctacc gcgacctcct cttcacgacg gacgggatcg ccaaccacat cagcggcgtc
     5881 atcctgtacg acgagacgat ccgtcagtcc tccctcgacg gcaccccgtt ccccaagctc
     5941 cttgagcgtc agggtgtcat ctccggcatc aaggtcgaca ccggcgcgaa gccgctcgcc
     6001 ctcgccgacg acggggagac gatcaccgaa ggcctcgacg gcctgcgcga ccggctcgcc
     6061 gagtaccacg gtctcggggc tcggttcgcc aagtggcgcg ccacctacac catcagcgac
     6121 aaccttccgt cgcagtacgc gatcgacgcg aacgccgatg cgctggcccg ctacgcggcg
     6181 ctctgccagg agatcggcat cgtcccgatc gtcgaacccg aagtgatgat ggagggcgac
     6241 cacaccatcc aacgcgccgc ggcggtcagc tccgccaccc tcgaggctct gtatcacgcc
     6301 ctctacgagc agcggatcca cctgccgggc agcctcctca agcccaacat ggtgctgtcg
     6361 ggatacgacg caccggtgca cgcgtcggtc gacgaggtcg ccgaccggac cctcgagtgc
     6421 ttcgctgccc gggtccccgc cgctgtcccg ggcatcgtgt tcctgtccgg cggacagagc
     6481 gacgaggacg ccaccgcaca tctcgacgcg atgaacaagc gcggacccca tccctgggag
     6541 ctcagcttct cctacggccg tgcgctccaa gcggctcccc tgcacgcctg gcgaggcgac
     6601 ccggccaacc ggacctcggc gcaagccgcg ttcctgctgc gggccaagct caatagcctc
     6661 gcccgttccg gcaagtactc acccgacatg gagcagcaag cggcctgagc accgaccggc
     6721 acccgggtcg tgcgggcgct cgtccaaaga gtcactgagg cccgcgtcac gatcgacggt
     6781 gccgtcgtcg gcgagatcgg ggtcggcttg tgcgtgttcg ccggtgtgac ccatggcgac
     6841 ggtgagcccc aagcccgacg gctggccgag aagctgtggc agcttcgggt cttccccgac
     6901 gacgacgggg tgatgaaccg ctcgctcgct gagaccaccg gcgccgcgct cgtcgtcagc
     6961 cagttcaccc tctacgccga cacccgccgg ggtcgccggc cgtcctgggc cgcggcggcc
     7021 cctcccgcgc gggctgcacc actggtggcg tcgctgacac acgcgctccg cgagctcggt
     7081 gccaccgtgg ccacggggcg cttcggtgcc gacatgtcgg tcgagctcgt caacgacggt
     7141 cccgtcacgc tcctcctcga ggtctgatcc cgaccgagcc tcggacgcga cccccgtcgg
     7201 gccggttccc gcggcgaccg cggctcccgg gcacccattc gcgcgctccc gggcgcgcgc
     7261 caccgaccaa ccgaccgcgc cgggcgctca tcggtcccga acacctgtgc ggggtcgacg
     7321 cgggtcgccg gtacgatgga tcggtcatga ccgacgttcc gaccgcgtcc gcaccgtcgg
     7381 tgggggaacg ggacgacatc gaacggctgc tcggtggcct caacccggtg caacgcgaag
     7441 cggccgccgc ccccgacggg ccgctcctga tcgtcgccgg cgccggctca gggaagaccc
     7501 gggtcctcac gcaccggatc gcctatctga tcgccaccgg acgggtctca cccttcaacc
     7561 tgctcgccat cacgttcacc aacaaggcgg cgggggagat gaagcaacgc gtcgcgacgc
     7621 tcgtcggccc ggtcgcccac cgaatgtggg tctcgacctt ccactcggcc tgcgcccgga
     7681 tcctgcgacg cgaggcggct cgagtcggtc ttcgttcctc gttcacgatc tacgaccagg
     7741 ccgacgcggt gcggctcacc gactacgtgc gtcgcgatct caacttggac ccgaaacggt
     7801 tcccgccccg tcagctccac gcccggatct tggcgttgaa gaacgacctc gttggacccg
     7861 acgaggcgct cgccgcggca gtgacgccac ccgaacgacg ctacgccgag atctacgttg
     7921 aatatcagcg ccggctcctc gaagcctccg cggtcgactt cgacgatctc ctgctcctgg
     7981 cggtgcgcct gttccgcgat caccccgacg cgctgacccg ctggcagcac cgtttcgccc
     8041 atgtactcgt cgacgagttc caggacacca acgctgcgca atgggagctc gtccgcctcc
     8101 tcaccgccgg atcccgcaac gtgatggtgg tcggtgacca tgaccaggcg gtctacggat
     8161 tccggggcgc cgactaccgc aacctggtcc gcttcgaaga gacgttcccg gacgccacgg
     8221 tgatcgtgct cgagcagaac taccgctcga cgcaggccat cctcgacgcc gccaacgccg
     8281 tgattgccaa caacgccgcc cggcagccca agcatctctg gaccgaacag atcgggggcg
     8341 agctcctcac ccagtaccag gcggaggacg aacacgac
//
LOCUS       DATCCH010000016         4500 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_42730, whole genome shotgun sequence.
ACCESSION   DATCCH010000016 DATCCH010000000
VERSION     DATCCH010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4500
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42730"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..1554
                     /locus_tag="VIJ44_00370"
     CDS             136..1554
                     /locus_tag="VIJ44_00370"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEY5164379.1"
                     /translation="MDVPSRLRAYYEAGSPTRQLADRLAGAGHECYLVGGTVRDAFLG
                     ATSPDVDLATDARPEEIRGLLDGWADHVWLQGERFGTVGAQKDGVHFELTTYRGDVYR
                     PESRKPTVTFADRIEDDLARRDFTINAMALQLPVPVLIDPHDGAADLAARRLRTPVEP
                     EVSFEADPLRMLRAARFVAAFALEPVPELVAALHAMHHRMTIVSPERIRDELCRLLGV
                     ADPAAGLWMLCETGASDEFIPELNAMRLEQDPIHSHKDVLAHTIAVVGNTDPGHPLVR
                     LAALVHDIGKPKTRSIGPGGVSFHHHEVVGARMTEERLRALRFSNETVHDVTKLVYLH
                     LRIHTYAMGWTDRAVRRYVRDAGPLLNELNELQRADCTTRNQRKAAALARRMDDLEVR
                     IASLRAREELDAIRPPLDGRQVMAYLGISPGPAIGEALDTLLEARLDEGPIDVDDAYD
                     RLEAWAHAHDIEPARKRIPPQT"
     gene            complement(1578..2660)
                     /locus_tag="VIJ44_00375"
     CDS             complement(1578..2660)
                     /locus_tag="VIJ44_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEY5164380.1"
                     /translation="MSPASKKVRVAIAGIGNCSSSLVQGVAYYGDASTEDEVPGLMHV
                     VLGGYHVGDVDFVAAFDVDAAKVGLDLSKAIFSGSNNTIKFAEVSALGVQVQRGPTFD
                     GLGEFYRDEVEESPAEPVDVAQALRDAKADVLVSYLPVGSEDAQRHYASACLEAGVGF
                     VNAIPVFIASDPTWAKKFEDAGVPIVGDDIKSQVGATIVHRILTRLFEDRGVELDRTY
                     QLNFGGNMDFKNMLERKRLKSKKISKTQAVTSQLDHKIEADNVHIGPSDHVPWLDDRK
                     WCYIRMEGRNFGDVPLNLELKLEVWDSPNSAGVIIDAVRCAKLALDRGVGGPLLGPSA
                     YFMKSPPVQYRDEEAREMVEAFAATT"
     gene            complement(2657..3307)
                     /locus_tag="VIJ44_00380"
     CDS             complement(2657..3307)
                     /locus_tag="VIJ44_00380"
                     /inference="COORDINATES: protein motif:HMM:NF021886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEY5164381.1"
                     /translation="MLELAVLGLLKDQPLHGYELKKRLGETLGFLWGVSYGSLYPALR
                     RLERTGAIAIVIPTVAPVPSPSTGSLSGDLAAARLKAMAGRLSASGRRTRKAYQITAA
                     GETLFAELLLADDPHSDDEKAFALKLAFCRHLEPVQRIELLDRRRRVLAERLDRQRQV
                     HGIRDDRYTRSLMEHRTKSTQRDLEWVDELITAERAGPGGSPPDAASPAVKEEASA"
     gene            3466..3831
                     /locus_tag="VIJ44_00385"
     CDS             3466..3831
                     /locus_tag="VIJ44_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015805702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5318 family protein"
                     /protein_id="HEY5164382.1"
                     /translation="MRRHVDYALARRAVLRDFRAGRLERDDVCDAHPELLRAGRHIGS
                     PAPTACPVCGRRNLRLVSYVYGDALARANGRCITSPTELDRLDAAHEEFTRYVVEVCL
                     DCHWNHLQRHELHGRRHVG"
     gene            3925..>4500
                     /gene="panB"
                     /locus_tag="VIJ44_00390"
     CDS             3925..>4500
                     /gene="panB"
                     /locus_tag="VIJ44_00390"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEY5164383.1"
                     /translation="MTEPSAPPRKVTAPVVTAAKETDTPLVMVTAYDAPSARIVDAAG
                     VDMILVGDSVAMTVLGYDDTLQVTTTDIAHHVAAVARTRPRALVVADLPWMSYHVSTE
                     DTVRNAATLIRAGASAVKLEGGRKRLAAVRAILDAEIPVMGHLGLTPQSFHAIGGFRV
                     QGRDLEAAREIVDDAIALGEAGCFSIVLECVP"
BASE COUNT          728 a         1664 c         1436 g          672 t
ORIGIN      
        1 ccggtcaccg caccggcctg agcgccggcc gagccgaatc gccgaacccc cgacccggcg
       61 agtcgggccg gatcccacgg ccgcgaggcg aaggaccgga tacctgggcg gcccgcccgc
      121 gacaatggtg accgcatgga cgtcccctcg cgcttgcgcg cgtactacga ggcgggctcg
      181 cccacccgcc agcttgccga tcgcctcgcc ggggccggcc acgagtgcta cctcgtgggc
      241 ggcaccgtcc gcgacgcgtt tttgggcgcc acctcgcccg acgtggatct cgccaccgac
      301 gcccgccccg aggagatccg cgggctcctc gacggctggg ccgaccacgt ctggctgcaa
      361 ggtgagcggt tcgggaccgt cggcgctcag aaagacggcg tgcacttcga gctcaccacc
      421 taccggggcg acgtgtatcg gcccgagagt cgcaagccca cggtgacgtt cgccgaccgg
      481 atcgaggacg acctcgcccg ccgcgacttc accatcaacg ccatggcgct gcagctaccg
      541 gtcccagtcc tcatcgaccc gcacgacggt gccgccgacc tcgcggcgcg ccgattacgc
      601 acccccgtcg aaccggaggt gtcgttcgag gccgacccgc tgcggatgct gcgggcggcg
      661 cggttcgtcg ccgcgttcgc cctcgaaccg gtccccgagc tcgtcgccgc gctgcacgcg
      721 atgcatcatc ggatgaccat cgtgagcccc gaacggatcc gcgacgagct gtgccgcctg
      781 ctcggcgtcg ccgatccggc cgccgggctc tggatgctgt gtgagaccgg cgcctcggac
      841 gagttcatcc ccgagctgaa cgccatgcgt ctcgagcaag acccgatcca cagccacaag
      901 gacgtcctcg cgcacaccat cgcggttgtc gggaacaccg accccggcca tcccttggtg
      961 cgcctcgccg cgttggtcca cgacatcggg aagccgaaaa cgcgctcgat cggaccgggg
     1021 ggagtgagct tccatcatca cgaggtcgtc ggcgcccgca tgaccgaaga gcggctccgg
     1081 gccctgcggt tcagcaacga gacggtgcac gacgtcacca agctcgtgta cctgcacctg
     1141 cggatccaca cctacgccat gggctggacc gaccgggcgg tacgccggta cgtacgagac
     1201 gccgggccgc tcctcaacga gctcaacgag ctccagcggg ccgactgcac cacccgcaac
     1261 cagcgcaaag ccgcggccct ggcccgccgc atggacgacc tcgaggtccg catcgcttcc
     1321 ctgcgggccc gagaagagct cgacgccatc cggccgccgc tcgacggccg ccaggtgatg
     1381 gcctacctgg gtatctcgcc cggtcccgcg atcggtgaag ccctcgacac cctgctggag
     1441 gctcgcctcg acgaaggccc gatcgacgtg gacgacgcct acgaccgcct cgaagcctgg
     1501 gcccacgccc acgacatcga accggcacgg aaacggatcc cgccccaaac ctgacgttcc
     1561 ccgaaccggg gatgcgctca ggtggtggcg gcgaacgcct ccaccatctc gcgtgcttcc
     1621 tcgtcccggt actgcaccgg cggtgacttc atgaagtacg cgctcggccc gagcaacggg
     1681 ccgccgacac cgcgatccaa cgccagcttg gcgcaccgca ccgcgtcgat gatgacgccg
     1741 gccgagttcg gcgagtccca cacctcgagc ttcagctcca aattgagggg cacgtcaccg
     1801 aagttccggc cctccatccg gatgtagcac cacttgcggt catcgagcca cggcacatgg
     1861 tccgacgggc cgatgtgcac gttgtccgcc tcgatcttgt ggtcgagctg gctcgtcacc
     1921 gcctgggtct tggagatctt cttggacttc agacgctttc gctccaacat gttcttgaag
     1981 tccatgttcc cgccgaagtt cagctggtac gtccgatcga gctccacgcc gcgatcctcg
     2041 aagaggcggg tcaggatgcg atgcacgatc gtggcgccca cctggctctt gatgtcgtca
     2101 ccgacgatgg ggacgccggc gtcctcgaac ttcttcgccc aggtcgggtc gctcgcgatg
     2161 aacaccggga tcgcgttgac gaatccgaca ccggcctcga ggcacgccga cgcgtagtgg
     2221 cgctgtgcat cctcggagcc gaccgggagg tagctgacga gcacgtcggc cttcgcgtcg
     2281 cgcagagcct gcgccacgtc gacgggctca gccggcgatt cttcgacctc gtcccggtag
     2341 aactcgccga gaccgtcgaa cgtcgggccg cgctggacct gcaccccgag ggcgctgacc
     2401 tcagcgaact tgatcgtgtt gttgctcccg ctgaagatcg ccttgctgag atccaaaccg
     2461 accttcgcgg catcgacgtc gaaggctgcc acgaagtcga cgtcgccaac gtggtagccg
     2521 ccgagcacga cgtgcatgag gcccgggacc tcgtcttcgg tggaggcgtc gccgtagtag
     2581 gcgacaccct gcaccaggct gctggaacag ttgccgattc cggcaatcgc gacgcgcact
     2641 ttcttcgatg ctgggctcat gcggacgctt cctccttgac ggcggggctc gcggcgtccg
     2701 ggggagaacc gcccgggccc gcgcgctccg cggtgatcag ctcgtcgacc cactcgaggt
     2761 cgcgctgggt ggacttcgtt cggtgctcca tcaaggagcg ggtgtagcgg tcgtcgcgga
     2821 tcccgtggac ctggcgctgc cggtccagcc gctcggccag aacccgacgc cgacggtcca
     2881 gcaactcgat ccgctgcacc ggctcgagat gtcggcagaa ggcgagcttc agggcgaatg
     2941 ccttctcgtc atcgctatgg ggatcatcgg ccaacagcag ctcggcgaag agggtctcgc
     3001 ctgccgcggt gatctggtac gccttgcggg tccgacggcc cgaagccgac agtcgaccgg
     3061 ccatcgcctt gaggcgagcc gcggccaggt cacccgacag cgaacccgtc gacgggctcg
     3121 gaaccggcgc caccgtcggt atgacgatcg cgatcgcccc cgtgcgctcc agtcggcgca
     3181 acgcgggata gagggagccg tacgagactc cccacaggaa cccgagcgtc tcgccgagac
     3241 gcttcttaag ctcatagccg tgcaagggct gatccttgag cagtccgagg acagctagct
     3301 ccagcacaag cactcctaaa aaggtgccga tatatcgaac cgatatatcg gaacacagta
     3361 tgagggatcc cggaggctcg gtcaaggcgg gcgcctcgct ccgggcgggc gcaccgatcg
     3421 gtgagaaccg gagatcgggc tgggggccct gcctacactc ggcccgtgcg gcggcacgtc
     3481 gactacgcgt tggcccgacg ggcggtcctg agggacttcc gcgccggtcg cctcgagcgc
     3541 gacgacgtct gcgacgccca tccggagctc ctgcgggccg gtcgccatat cgggtccccg
     3601 gccccgaccg cctgcccggt ctgcggacgg cgcaacctcc gcctcgtctc ctacgtctac
     3661 ggcgacgccc tcgcccgtgc caacggtcgc tgcatcacct ccccgaccga gctcgaccgg
     3721 ctcgacgcgg cccacgaaga gttcactcgc tatgtcgtcg aggtctgcct tgactgccac
     3781 tggaaccatc tccagcgcca cgagctccac ggccgccgac acgtcggctg acctcgcacc
     3841 ggaccccggc cgccacgacc gcgagcgggt aacctcgccg tgccgccggg agccgcacga
     3901 gccctggacg gcggaggtga ggccatgacc gagcccagcg cgccgccccg caaggtgacc
     3961 gctcctgtgg tcaccgcagc caaagagacc gacacccccc tcgtgatggt cacggcctat
     4021 gacgctccat cagcgcggat cgtggacgcc gccggtgtcg acatgatcct cgtcggggac
     4081 tcggtcgcca tgaccgtgct cggctacgac gacaccctgc aggtcaccac gaccgacatc
     4141 gcgcatcacg tcgccgcggt ggcgcgcacc cgtccccgag ccctcgtcgt cgccgacctc
     4201 ccgtggatga gctaccacgt ctccaccgag gacacggtcc gcaacgccgc caccctgatc
     4261 cgagccgggg cgagcgcggt caagctcgaa ggcggccgaa aacgactcgc cgcagtccgc
     4321 gccatcctcg acgccgagat cccggtgatg ggccatctgg gcctgacccc gcagtcgttt
     4381 cacgccatcg gagggttccg ggtccagggc cgagacctcg aggccgcccg ggagattgtc
     4441 gacgacgcca tcgcgctcgg cgaggccggc tgcttctcga tcgtcctcga gtgcgtcccc
//
LOCUS       DATCCH010000017         2953 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_415992, whole genome shotgun sequence.
ACCESSION   DATCCH010000017 DATCCH010000000
VERSION     DATCCH010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415992"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..968
                     /locus_tag="VIJ44_00395"
     CDS             <1..968
                     /locus_tag="VIJ44_00395"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEY5164384.1"
                     /translation="PAMSDTDLRRATDAVRGAERIALACHVTPDGDALGSMLGLFHVL
                     RTTRTDVVASFPSPFVVAPHYRELPGLELLTKPDDFPRTPDVMVTFDCGSLARLGDLE
                     GPATAARELIVIDHHISNDRYGTVNVIEPDAAASGWVVHRLIDELGLPLNRDAAVCLY
                     TAIVCDTGRFQYDTTTPEVFDLARRLTEFDIPVARLSRSLFEEHRFQYLQLLADALSH
                     AELVREQQFVWTAVTQTMLGHHDVTMDEVEGLIDILRRTQEAEVTCVLKEEPDGTVRV
                     SLRSLGAVDVRRIAEAHGGGGHRYAAGFTSELDIPTTVARIRAAL"
     gene            980..1873
                     /gene="truB"
                     /locus_tag="VIJ44_00400"
     CDS             980..1873
                     /gene="truB"
                     /locus_tag="VIJ44_00400"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEY5164385.1"
                     /translation="MRDGLVVVDKPGGCTSHDVVARLRHVYRQRRVGHAGTLDPDATG
                     VLLVGLGRVTRLLRFLAETGKTYRGDVVLGVATSTLDAAGSVLDRKPMSVTRAQLDEV
                     ARRFVGDLQQVPPMVSALKVGGRRLHDLARHGEEVERPPRPVRIDRLDIEHFEPGPYP
                     VATILVECSTGTYVRSLAADLGAALGGCAHLGALRRLRVGSFDVSEAHTLDTIAAAPD
                     DVLLTPAIAMRDLERLDVDDEQARAVRHGMTFPCSTLPAPLGTGPFAVADERGDLLAV
                     YERRHAGIKPAVVVAAGPDGT"
     gene            1876..2832
                     /locus_tag="VIJ44_00405"
     CDS             1876..2832
                     /locus_tag="VIJ44_00405"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEY5164386.1"
                     /translation="MRVVRDLTTLEAPADGSVVTIGAYDGVHLGHQAVLRLVRELATA
                     RGHDAVCVTFDRHPAEVVRPDSAPCLLTTLEQKLELLDATGYLDTAVVIAFDEERSHE
                     PAEGFVHEVLVAGLQARVVVVGADFHFGYRRHGTVRLLEQMGAELGFEVVGLGLVPVI
                     ADPGLPYSSTAVRALLSDGRVADVAALLGRPPRPYELRGSVVTGDRRGRTLGYPTANV
                     DVPTRQCLPADGIYAGTFVAEDGVERVGAISLGRRPTFHADATRSLLEVHVLDFDGDL
                     YGQTVKVRVHERLRGEERFDSVDALIAQMRHDVETTRRIVTV"
BASE COUNT          400 a         1165 c          928 g          460 t
ORIGIN      
        1 caccagccat gagtgacacc gacctccgcc gcgccacgga cgcggtccgc ggcgccgagc
       61 ggatcgcgct cgcgtgtcac gtcacccccg acggcgacgc gctcgggtcg atgctcggtc
      121 tctttcatgt cctgcgcacc acgagaaccg atgtcgtcgc gtcgttcccg tccccgttcg
      181 tcgtcgcgcc ccactatcgg gagctcccgg gactggagct cctcaccaag cccgacgact
      241 tcccccgcac acctgacgtc atggtcacct tcgactgcgg ttccctcgcc cgcctcggtg
      301 acctcgaagg ccccgccacc gcggcacgcg agctgatcgt catcgaccat cacatctcca
      361 acgaccggta cgggacggtc aacgtcatcg aaccggatgc cgccgcgagc ggctgggtcg
      421 tgcatcgcct catcgacgag ctgggattgc cgctcaaccg tgacgccgcg gtctgcctgt
      481 acaccgcgat cgtgtgcgat accggccgct ttcagtacga caccacgacc cctgaggtgt
      541 tcgacctcgc tcgccgcctc accgagttcg atatcccggt cgctcgtctg tcgcgctcgt
      601 tgttcgagga gcaccggttc cagtacctcc agctcctcgc cgacgcgctc agccacgccg
      661 agctggtccg cgagcagcag ttcgtgtgga ccgcggtgac ccagaccatg ctgggccatc
      721 atgatgtgac gatggacgag gtcgaaggcc tgatcgacat cttgcgtcgc acccaggagg
      781 ccgaggtgac ctgcgtcctc aaggaggaac ccgacgggac ggtgcgggtg agcctccgct
      841 ccctcggtgc cgtcgacgtt cggcgcatcg ccgaagccca tggcggtggc ggtcaccggt
      901 acgcggccgg cttcacctcc gagctcgaca tccccacgac ggtggcgcgc atccgcgccg
      961 ccctctagct ctgccgccga tgcgcgacgg actcgtcgtc gtcgacaagc cgggcggctg
     1021 cacgtcccac gacgtcgtcg cgcgcctccg ccatgtctat cggcagcggc gcgtcggcca
     1081 cgccggcacc ctcgatcccg acgccaccgg tgtcctcctc gtgggcctcg gacgggtcac
     1141 ccgcctgttg cgcttcctcg ccgagaccgg caagacctac cggggtgacg tggtcctcgg
     1201 tgtcgcgacc agcacgctcg acgccgccgg ctcggtcctg gaccgcaaac cgatgtcggt
     1261 cacccgcgcg cagctcgacg aggtcgctcg ccgcttcgtc ggcgacctcc agcaggtccc
     1321 accgatggtc agcgcactca aggtcggcgg ccgccgtctc cacgacctcg cccgccacgg
     1381 cgaagaggtc gagcggcccc cgcgtccggt gcgcatcgac cgtctcgaca tcgaacactt
     1441 cgagccgggt ccgtacccgg tggccaccat cctcgtcgag tgcagcaccg gcacctacgt
     1501 gcggtccctc gccgcggacc tcggcgcggc gctggggggc tgcgcccacc tcggcgcgct
     1561 tcgccgcctc cgggtggggt cgttcgacgt cagcgaagcg cacaccctcg acacgatcgc
     1621 cgccgctccc gacgacgtgc tgctcacgcc ggccatcgcg atgcgggacc tcgaacgcct
     1681 cgacgtcgac gacgagcagg cccgcgccgt tcgccacggg atgacgttcc cctgctcgac
     1741 gttgccggca ccgctgggta ccgggccctt cgccgttgcc gacgagcgcg gcgacctgct
     1801 cgcggtctac gagcgccgcc acgccgggat caaaccggcg gtggtggtcg ccgccgggcc
     1861 cgacggaacc tgaccgtgcg cgtcgtccgc gacctcacca ccctcgaggc ccccgccgac
     1921 ggttcggtgg tcacgatcgg tgcctacgac ggcgtccatc tcggccacca ggctgtcctc
     1981 cgtctcgtcc gcgagctcgc caccgcccga ggccatgacg cggtgtgcgt gaccttcgac
     2041 cgccaccccg ccgaggtggt ccgacccgac agcgccccgt gcctgctcac caccctcgag
     2101 cagaagctcg agttgcttga cgccaccggg tacctcgaca ccgccgtggt catcgcgttc
     2161 gacgaggaac gcagccatga gcccgccgag ggcttcgtcc acgaggtcct cgtggccggg
     2221 ctccaggcgc gcgtcgtcgt cgtcggcgcc gacttccact tcgggtaccg ccgccacggc
     2281 acggtccggc tgctcgagca gatgggcgcc gaactcggct tcgaggtcgt cggcctcggc
     2341 ctcgtcccgg tcatcgccga tccggggctc ccgtactcgt cgaccgccgt ccgcgccctc
     2401 ctgagcgacg ggcgtgtcgc cgatgtcgcc gcgctcctcg gccgcccgcc ccgcccgtac
     2461 gagctgcgcg gcagcgtcgt caccggtgac cgtcgcggcc gcaccctggg gtacccaacc
     2521 gcgaacgtcg acgtcccgac ccgtcagtgc ctccccgccg acgggatcta cgccggcacc
     2581 ttcgtcgccg aggacggcgt cgaacgggtt ggggccatct ccctcggccg ccgcccgacg
     2641 tttcacgccg acgccacccg atccctcctc gaagtgcatg tgctcgactt cgacggtgat
     2701 ctctacggcc agaccgtcaa ggtgcgggtc cacgaacgcc tccgcggcga ggaacgcttc
     2761 gactcggtcg acgcgctcat cgcccagatg cgacacgacg tcgagacgac ccgccgcatc
     2821 gtgaccgtct gagcaccacc acccgcgccc ggtcgtggac cgcgcgccgc tccgcccgtc
     2881 gggggtgctc gtggctcgcc aaggtcattt tgatgaagcc cagcagctcc ggcttgaggc
     2941 tggtgagcgg ttc
//
LOCUS       DATCCH010000018         5366 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_86055, whole genome shotgun sequence.
ACCESSION   DATCCH010000018 DATCCH010000000
VERSION     DATCCH010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5366
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86055"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(442..1266)
                     /locus_tag="VIJ44_00410"
     CDS             complement(442..1266)
                     /locus_tag="VIJ44_00410"
                     /inference="COORDINATES: protein motif:HMM:NF024141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5164387.1"
                     /translation="MTTTTPTVDRPTSPGLSSVFGATLRSEWTKFRSVRSTVWTLLAA
                     AVATVGLGAALCAAYVSRYDQLTLGERARFDPMSFNLRGLFLAQLAIGVLGVLVISSE
                     YTTGMIRTSFAAVPQRRTVISAKSFVFLVVAFVVGVVACFGAFVAGQSILASKHLNVG
                     LSDPGVLRAIAGGGVYLALIGLLGLAFGTLLRRTAGAIAALFGLVLVLPPLASVLPSP
                     WGTDIPKYLPSDAGQSMLGIHYHADLLSPGAGFAVLVGYAVAAFGLSLLVVARRDA"
     gene            complement(1269..2204)
                     /locus_tag="VIJ44_00415"
     CDS             complement(1269..2204)
                     /locus_tag="VIJ44_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005163671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5164388.1"
                     /translation="MIEARGLTKRYESTVAVDDLSFDVRPGHVTGFLGPNGAGKTTTM
                     RLILGLDRPNKGTVAVNGRSYADHRFPLHEVGALLDAHALHGGRRAYDHLLCLAQSNR
                     IPRRRVGEVLDAVGLTDVAGRRAGTFSLGMGQRLGIAAALLGDPGILIFDEPVNGLDP
                     EGILWIRTLLKSLASEGRTIFISSHLMSEMSLTADRLIVIGRGRLIADAPIGELTGRA
                     TGQVRVRSPRRDELVALLERHGATVERTEPDALVVRGATAATIGDLAAEQQLALHELT
                     PELASLEETFFELTNDSVDFRAEVPGPAASSATGA"
     gene            complement(2435..2902)
                     /locus_tag="VIJ44_00420"
     CDS             complement(2435..2902)
                     /locus_tag="VIJ44_00420"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY5164389.1"
                     /translation="MTKVTKAEVARDVWQQLVGYFFAHRESQMRAAQELGLTPGHMKT
                     LFELDGSEGRSMGDLAVLLVCDASNVTWLVDRLEERGLVERRPHPRDRRVKSVVLTPE
                     GVKTKALLIERLLTPPDDLVGLDRADLDALGAALELLPPHPPFWSAEGEPAAG"
     gene            3011..3973
                     /locus_tag="VIJ44_00425"
     CDS             3011..3973
                     /locus_tag="VIJ44_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007266117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5164390.1"
                     /translation="MPEPDAAIRAVDLAKSFGAVRALEGISLEVPTGTVLGLLGPNGA
                     GKTTAVRILTTLLKPDRGLAEVLGRDVTEYPMRTRASIGLAGQNAAIDENLTARENLR
                     LVGRLTHVPAPEIRSRTETLLERFDLVGAADRLARTFSGGMRRRLDLAAALVHQPPVL
                     FLDEPTTGLDPQGRTELLAFIEELVDGGTTLLLTTQYLEDADRLANRIVVIDHGAVIA
                     EGTPSDLKARLGATVIEVAFGDDATTRRAATQLARLGDLAVDGKEVRVSVADGPRAML
                     EAVRALDTEQLEPLTMALREPTLDDVFLTLTGHAADTEPEATRP"
     gene            3970..4785
                     /locus_tag="VIJ44_00430"
     CDS             3970..4785
                     /locus_tag="VIJ44_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5164391.1"
                     /translation="MTATAATLPAPTGSSVSYARPRVVGDSLTIAWRNLLNIRRNPQL
                     LVFATIQPVIFVLMFRYVFGGAIKVPGVSYVDYLMPGIFVQTVVFGSLTTGVGLAEDL
                     QKGLVDRFRSLPMARSAVLIGRTLADLVRNVFVVALMCIVGLLVGWNPSASLPAILAG
                     LGLVLAFSYSLSWLFAIVGLAVRNGETAQAASFPLLAPLVFASSAFVPVASMPDWLQV
                     FAKHQPVSVVVNAVRGLTLGSATTSTVLSALAWIVGLVAVFAPIAVARYRRAV"
     gene            complement(4879..>5366)
                     /locus_tag="VIJ44_00435"
     CDS             complement(4879..>5366)
                     /locus_tag="VIJ44_00435"
                     /inference="COORDINATES: protein motif:HMM:NF025600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YHYH protein"
                     /protein_id="HEY5164392.1"
                     /translation="DRNPNHIAAQSITWSLPADPTAASAPSCTNGGAIGVLTDGVVLF
                     NALDGEGRDAAAHEVLDGCGGHPEMSSTYHHHEVPSCILDQATGRSTLVGYAKDGYGI
                     YAERDSHGTLLTNASLDVCHGRTSTVTWNGKKKKLYHYDATLEYPYTLGCFHGTPIST
                     H"
BASE COUNT          810 a         1898 c         1815 g          843 t
ORIGIN      
        1 cctcgaacgc cgccgccgag cgttcgtcct ggcgttcgcg tgcggatgtg tcctctccag
       61 cgtctacggc ttctggtccg gggcgtggcc gttcggggtg gtcgagctgg tgtggtccgg
      121 gatcgcggtg cggcgctacc tcaccgcgcc agccgagccg gtctgagccg gatggcaacg
      181 ccgagggcac ggttcgcctg aaggcacggc ggctctcccg gcgcggacct gcggtcgtgc
      241 tgctcgtcgc cgcggtcatc tacctcagca cgcagtgtca gagcctgccg gtggtgcttc
      301 ccggccggga ggccggctcg acccattatc ggatcgggta cgccgcggtg gcgttcgtcc
      361 tcgccgcgct ggcagcgtgg ggagcgcgac ggacggcccg ctgcgcgttg acacgacgag
      421 ccacggccga cgcggcgacg attaggcgtc gcgtcgggcc acgacgagca gggacagacc
      481 gaacgcagca accgcgtagc cgacgagcac cgcgaacccc gccccggggg agagcagatc
      541 ggcgtggtag tggattccga gcatcgactg cccagcgtcg ctcggaaggt acttcgggat
      601 gtccgtgccc cacggtgacg ggaggacgct ggcgagcgga ggcaggacca gcaccagccc
      661 gaacaaggcg gcgatggcac ccgcagtgcg gcgcagcaac gtcccgaacg ccaggcccag
      721 cagaccgatc aacgccaggt agaccccgcc accggcgatg gcgcgcagga cgccgggatc
      781 actcaggccg acgttgaggt gcttcgaagc caggatcgac tgaccggcga cgaaggcacc
      841 gaagcaggcc acgacgccga cgacgaacgc gaccacgagg aacacgaagc tcttggccga
      901 gatgaccgtt cgccgctgcg ggacggcggc gaagctggtc cggatcatcc cggtcgtgta
      961 ctcggagctg atgacgagca ccccgagcac gccgatcgcc agctgggcga ggaacaggcc
     1021 ccgcaggttg aagctcatcg ggtcgaaccg agcccgctca ccgagggtga gctggtcata
     1081 acggctcacg tacgcggcgc acaacgccgc gccgaggccg acggtcgcga ccgcggcggc
     1141 gagcagcgtc cagaccgtcg agcggacgga gcggaacttc gtccactctg atcgcagcgt
     1201 ggcgccgaag acgctcgaca gcccggggga ggtcggccgg tcgacggtcg gtgtggtcgt
     1261 cgtcatgctc aagctcctgt cgcgctcgac gccgcgggcc caggcacctc ggcacggaag
     1321 tcgacgctgt cgttggtcaa ctcgaagaac gtctcctcga gggacgccag ctcgggggtc
     1381 agctcatgga gggccagctg ctgctcggcc gcgaggtcgc cgatcgtcgc cgcggtcgcc
     1441 ccccgcacga ccagcgcgtc gggttcggtc cgctcgacgg tggcgccgtg acgctcgagg
     1501 agcgcgacga gctcgtctcg tcgcggtgac cggacccgga cctgaccggt ggctcgcccg
     1561 gtcagctcac cgatcggcgc gtcggcgatg agccgaccgc ggccgatcac gatcagacgg
     1621 tcggcggtga gcgacatctc gctcatgaga tgactcgaga tgaagatcgt gcggccctcg
     1681 ctggccaggg acttgaggag ggtccggatc cacaggatgc cctcggggtc gagtccgttg
     1741 accggctcat cgaagatcag gatcccgggg tcaccgagga gcgccgcggc gatcccgagc
     1801 cgctgaccca tgcccaggga gaaggtgccg gcccgtctcc cggcgacgtc ggtgagcccc
     1861 acggcgtcga gcacctcgcc cacccggcgg cgggggatcc gattgctctg ggcgagacac
     1921 aacagatggt cgtaggcccg gcgtccgccg tggagcgcgt gggcgtcgag cagggcaccg
     1981 acctcgtgga gcgggaagcg gtggtcggca taggaacgcc cgttgaccgc aacggtgccc
     2041 ttgttgggcc ggtcgagacc caaaatcagc cgcatcgtcg tcgtcttgcc ggcaccgttc
     2101 ggaccgagga agcccgtgac gtgcccggga cgcacgtcga agctcaggtc gtcgacggcg
     2161 acggtgctct cgtaccgctt ggtgaggcca cgtgcctcga tcatcagctg atcctctcgt
     2221 tcgctcggct cggacgatac gcggcctcac aggcggcgac gacgcccaca agcaggaatt
     2281 cgggggcatc cccaaggacg atccggcctc gtctccgggt gacgccgaaa tgttcgtgga
     2341 cccgtctcac atcctcacca agattgggtt gcaagacccg ggcgcaagcc gtcgtcgacg
     2401 cctccggaac cggtctcgtc cgacccgcca accgtcagcc ggcggcgggc tcgccctccg
     2461 cgctccaaaa cggcgggtga ggcgggagga gctccagggc ggcgcccagc gcgtcgaggt
     2521 cggcgcggtc cagcccgacc aggtcgtcgg ggggcgtcaa gagccgctcg atgagcagcg
     2581 ccttcgtctt cacgccctcg ggggtgagca ccaccgattt cacgcgacgg tctcgggggt
     2641 gggggcggcg ctccacgagg ccgcgctcct cgagccggtc gacgagccac gtgacgttcg
     2701 acgcgtcgca caccagcagc accgcgaggt cgcccatcga gcggccttcg gatccgtcga
     2761 gctcgaagag cgtcttcatg tgcccggggg tcaggccgag ctcttgggcg gcgcgcatct
     2821 gtgactcccg gtgcgcgaag aagtagccga cgagctgttg ccagacgtcc cgagcgacct
     2881 ccgctttggt gaccttcgtc acgtgcaaga ccatacgcct tcctcgagta accccaaggg
     2941 ttgaagcacc gataatctcg gggtgctgcg atatttgatc tccgtcaagg ataacgcgag
     3001 gatgacgccc atgcccgaac ccgacgccgc catccgcgcc gtggatctcg ccaagtcgtt
     3061 cggcgccgtc cgcgccctcg aggggatctc cctcgaggtg ccgaccggta cggtgctcgg
     3121 cctcctcggc ccgaacggtg ccgggaagac caccgcggtc cgcatcctca cgaccctgtt
     3181 gaagccggac cgaggcctcg ccgaggtcct cggccgagac gtcaccgagt acccgatgcg
     3241 cacccgcgcc tcgatcgggc tcgccggcca gaacgccgcc atcgacgaga acttgaccgc
     3301 ccgtgagaac ctccgccttg tcggccgcct cacccacgtc cccgcccccg aaatccggtc
     3361 ccggaccgag acgctcctcg agcgcttcga tctcgtcggc gccgccgatc gcctggcacg
     3421 aaccttctcc ggtgggatgc gccgccgtct cgacctcgcg gcggcgctcg tccaccaacc
     3481 tccggtcctg ttcctcgacg aacccaccac cggcctcgac ccccaaggcc gcaccgagct
     3541 gttggccttc atcgaagagc tcgtcgacgg gggcaccacg ctgctcctca ccacccagta
     3601 cctcgaagac gcggaccggc tcgcgaaccg gatcgtcgtg atcgaccacg gcgcggtgat
     3661 cgccgaggga accccgagcg acctcaaggc gcgtctcggc gccaccgtca tcgaggtcgc
     3721 cttcggcgac gatgccacca cccgtcgcgc cgccacccag ctggcgcgcc tcggagatct
     3781 cgccgtcgac gggaaggaag tgcgggtcag tgtcgccgac ggtccccggg cgatgctcga
     3841 agcagtgcgg gccctcgaca ccgagcagct tgagccgctc acgatggcgc tgcgcgagcc
     3901 gaccctcgac gacgtcttcc tcacgctcac cggccacgcg gccgacaccg aaccggaggc
     3961 gacccgccca tgaccgctac cgcggccact ctccccgccc cgaccggatc cagcgtctcg
     4021 tacgcccggc cccgagtcgt gggcgattcg ctgaccatcg cctggcgcaa cctgctgaac
     4081 atccggcgca atccccaact gctggtgttc gccaccattc agcccgtcat cttcgtgctg
     4141 atgttccgct acgtgttcgg cggcgcgatc aaggtgcccg gcgtctccta cgtcgattac
     4201 ctgatgccgg ggatcttcgt gcagaccgtg gtctttggtt cgttgaccac cggggtcggc
     4261 ctcgccgagg atctccagaa gggcctcgtg gaccggttcc gttccctgcc gatggcacgc
     4321 tcggcggtcc tcatcgggcg caccctcgcc gatctggttc gcaatgtgtt cgtcgtcgcg
     4381 ctgatgtgca tcgtcggtct tctcgtcggg tggaacccca gcgccagcct tcccgcgatc
     4441 ctggcggggc tcggactggt cctcgccttc tcgtactcgc tctcgtggct gttcgccatc
     4501 gtgggcctcg cggtgcgcaa cggcgagacc gcccaggccg cgtcgttccc gctgctcgcc
     4561 ccgctggtct tcgcgtcgtc ggcgttcgtt ccggtcgcgt cgatgccgga ctggctccag
     4621 gtgttcgcca agcatcaacc ggtgtccgtt gtcgtcaacg ccgtgcgcgg cctcacgctc
     4681 ggcagcgcga ccacctcgac cgtcctgagc gcgttggcgt ggatcgtcgg tctcgtcgcg
     4741 gtcttcgccc cgatcgcggt cgctcgctac cgacgtgccg tgtagcgaga gggtcgccgc
     4801 ggctcgcgac cgcggctgat caaccgcggc gctctgcccc gacctggctt tctgcggcgc
     4861 tcggtgtgga agccgacgtc agtgggtgct gatcggtgtc ccgtggaagc atccgagcgt
     4921 gtacgggtat tcgagcgtcg cgtcgtagtg gtacagcttc ttcttcttgc cgttccacgt
     4981 cacggtgctg gtgcgcccgt gacagacatc caggctggcg ttggtgagga gagtcccgtg
     5041 cgagtcgcgc tcggcgtaga tgccgtagcc gtccttggcg tacccgacca acgtggatcg
     5101 ccccgtcgcc tgatcgagga tgcaggacgg gacctcatga tggtgatagg tcgagctcat
     5161 ctcggggtga ccaccacagc cgtcgaggac ctcgtgggcc gcggcgtctc gcccctcgcc
     5221 atccaacgcg ttgaacaaca cgacaccgtc ggtcagcaca ccgatcgccc cgccgttcgt
     5281 gcacgaggga gcactggccg cggtgggatc ggccgggaga ctccacgtga tcgactgggc
     5341 cgcgatgtgg ttcgggttgc ggtcgt
//
LOCUS       DATCCH010000019         2354 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_42896, whole genome shotgun sequence.
ACCESSION   DATCCH010000019 DATCCH010000000
VERSION     DATCCH010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42896"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1131
                     /locus_tag="VIJ44_00440"
     CDS             <1..1131
                     /locus_tag="VIJ44_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164393.1"
                     /translation="LAFRLALRLAQNRVRAALLTAAALAGLSTLWSERPLILGLVAFL
                     VVIWAVEVPDSAVGRRPLVVLPIVFWCWANVHGTFALGFAYLGLHLVGRWVEGHRPTE
                     GRERQLVVAAGVGFAVTFLNPYGVGLVTFPVDLLGRSDILRHVVEWASPDFHTAAGYA
                     FALWIVVFAAVAARASGRVSRRDLIVALPFLLLGLWALRNIAIAPLVGLPIAARAVAR
                     PRRPETPDRTGGLRLVLAATLTLVVVVLTMRAAAQPDFAFNGYPVSAMHAVQRDGLVG
                     RRLLTSDADAGYVILRYWPAQRVFLDDRYDMYPRPVITDYLTLSHARPGWAGVLARYD
                     VEVIVWQRGDPLVQLVEASGHWRSVYQDHSRVVLVRNDVRTT"
     gene            complement(1184..1381)
                     /locus_tag="VIJ44_00445"
     CDS             complement(1184..1381)
                     /locus_tag="VIJ44_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019959989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEY5164394.1"
                     /translation="MDLFVLRTWLRARFLRDERGANLVEYLLLVSLIAIAVIGAVKFF
                     GGQLSPKFNQAGSQLSSASGP"
     gene            complement(1590..2108)
                     /locus_tag="VIJ44_00450"
     CDS             complement(1590..2108)
                     /locus_tag="VIJ44_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010988409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY5164395.1"
                     /translation="MIEDLSDPRRTEPAYVLSERATLEDWLEFHRTTLLLKCEGLDDI
                     GRKRRPVPTSNLSLHGLVRHMTEVERNWFRRTLLCDHAAPWVWIDPEVEDSELVPLDD
                     ADWTADRAAWEAECETSRVSAAAHQLDDTGLRDGVPCSLRWIYVHLIEEYARHNGHAD
                     LIRELVDGQVGW"
     gene            2243..>2354
                     /locus_tag="VIJ44_00455"
     CDS             2243..>2354
                     /locus_tag="VIJ44_00455"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005448949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEY5164396.1"
                     /translation="MVMVALTGGIGSGKSTVAEMLARHGAVLVDADAIARE"
BASE COUNT          304 a          826 c          825 g          399 t
ORIGIN      
        1 ctcgccttcc ggctggcgtt gcggctggcc cagaatcggg tccgggccgc gttgctcacc
       61 gcggcggcgt tggccgggct cagcacgctg tggtccgaac ggccgctgat cctcgggctg
      121 gtggcgttcc tcgtcgtcat ctgggccgtc gaggtccccg actccgccgt cggtcgccgg
      181 ccgctggtgg tgctccccat cgtgttctgg tgctgggcga atgtgcacgg cacgttcgcg
      241 ctcgggttcg cgtacctcgg gttgcatctc gtcggccgct gggtcgaggg gcaccggccc
      301 accgagggtc gcgaacgcca gctggtcgtc gcggccgggg tcgggttcgc ggtgacgttc
      361 ctcaacccgt acggggtcgg gctggtcacg ttccccgtcg atctgcttgg ccggtccgac
      421 attctccgtc acgtcgtcga gtgggcttcc ccggactttc acaccgcggc cgggtacgcg
      481 ttcgcgctct ggatcgtcgt gttcgcggcg gtggccgccc gggcgtcggg acgggtgtcg
      541 cgccgcgacc tgatcgtggc gttgccgttc ctgctcctcg ggttatgggc gttgcgcaac
      601 atcgcgatcg cgcccctcgt cgggcttccg atcgcggcgc gggccgtggc gcgcccgcgt
      661 cgccccgaga ccccggaccg gacagggggg ctgcggctgg tcctcgccgc gaccttgacc
      721 ctcgtcgtcg tggtgctcac catgcgggcc gcggcccaac ccgacttcgc gttcaacggc
      781 tacccggtca gcgcgatgca cgcggttcag cgcgacgggc tggtcgggcg aaggctgctc
      841 acctccgacg ccgacgccgg gtacgtgatc ctgcgctact ggccggcgca gcgggtcttc
      901 ctcgacgacc ggtacgacat gtacccccgg ccggtcatca ccgactacct gacgttgtcc
      961 catgcgcgtc ccggatgggc gggcgtgctg gcccgatacg acgtcgaggt gatcgtgtgg
     1021 cagcgcggcg acccgctggt gcagctggtc gaggccagtg gacactggcg gtcggtgtac
     1081 caggaccaca gccgagtggt gctcgtccgc aacgacgtgc gaaccacctg accggcacgc
     1141 cgtcgcgccc cgccgggcga tcggcgtgcc ggccacgcgg gctctagggg ccagaggcgc
     1201 tgctgagctg actcccggcc tgattgaact tcgggctcag ctgcccgccg aagaacttca
     1261 ccgccccgat caccgcgatc gcgatcaggc tcaccaacaa caggtactcg acgaggttgg
     1321 cgccgcgctc atcccggagg aaccgggccc gcagccacgt ccggagcacg aagaggtcca
     1381 tcgcgccgtg tgcgtctcct gcgtccgtgc cccgtcgcgc ctggcctgcc gggttatcgg
     1441 caggtaaggg gaaggttctt gagcggtgcg gtcaaaacgt cgatgccgtc acccgacccg
     1501 gtccagctcc ggccgttccc ggccggcccc ggggctggaa ggtgcaggag ggcccaggcg
     1561 ggaccgtcgt ggctccggct ggtggcggcc taccagccga cctggccgtc gacgagctct
     1621 cggatgaggt cggcgtgacc gttatgccgc gcgtattcct cgatcaggtg gacatagatc
     1681 catcgcaacg agcacggcac accgtccctc agcccggtgt cgtcgagctg gtgagccgcg
     1741 gccgacaccc gactggtttc acactcggcc tcccacgccg ctcgatccgc cgtccagtcc
     1801 gcgtcgtcga gggggacgag ctcgctgtcc tcgacctcag gatcgatcca gacccagggg
     1861 gcggcgtggt cgcagagcaa ggtccgacga aaccagtttc gttccacctc ggtcatgtgg
     1921 cggaccaggc cgtgcaggga caggttcgag gtcggcaccg gccgccgctt gcgcccaatg
     1981 tcatcgagtc cctcgcactt gagcagcagc gtggtgcggt ggaactcgag ccaatcctcc
     2041 aaggtcgccc gttcggataa gacgtacgcg ggctcggtgc gtcggggatc cgacaggtcc
     2101 tcgatcacga caggtcctcc ccgttcaatc ctccggcggc ccaggcgcgt cgacagtagc
     2161 gacgcgacca ccacgctcac ggagcagctt tcaggctggc gcggccacgg gtcagggccc
     2221 tgagtccgta gcatccgtga ggatggtcat ggtggcgctc accggcggga tcgggtccgg
     2281 gaagtccacc gtcgccgaga tgttggcccg tcacggcgcg gtgctcgtcg acgccgacgc
     2341 catcgctcgc gagg
//
LOCUS       DATCCH010000020         2206 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_251724, whole genome shotgun sequence.
ACCESSION   DATCCH010000020 DATCCH010000000
VERSION     DATCCH010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251724"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="VIJ44_00460"
     CDS             <1..380
                     /locus_tag="VIJ44_00460"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012864280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY5164397.1"
                     /translation="GGPPAGACQARPVADEGINQPYGGPGGHPGIDLHASYGDPIHAC
                     RAGTIVSAGWMGGYGNATVIDHGGGMATLYGHQSQIVVSEGQHVNAGDVIGYVGSTGF
                     STGPHLHFEVRINGNTVDPAPYL"
     gene            477..662
                     /locus_tag="VIJ44_00465"
     CDS             477..662
                     /locus_tag="VIJ44_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164398.1"
                     /translation="MTTWEYMILSLPPFAAPTRLPTSSPALQALDEVGAQGWEAVSMT
                     VLASGEVAVLLKRPTRG"
     gene            904..1068
                     /locus_tag="VIJ44_00470"
     CDS             904..1068
                     /locus_tag="VIJ44_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164399.1"
                     /translation="MTVAELSALVTLGLGAAALVIVWVEAKVPGFSGLRFAPVPMPRR
                     RAHAENVHRS"
     gene            1065..1256
                     /locus_tag="VIJ44_00475"
     CDS             1065..1256
                     /locus_tag="VIJ44_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164400.1"
                     /translation="MIEARMSHGGVALYDSSSSRLVRWLMMSSGTPTGVEVDGDDVTV
                     TFADGERSVYDLNTGVLER"
     gene            1559..>2206
                     /locus_tag="VIJ44_00480"
     CDS             1559..>2206
                     /locus_tag="VIJ44_00480"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEY5164401.1"
                     /translation="MIILGPVVALGVILSQILSDGLAWYNLVIAAIFYFVVGHGVTVG
                     FHRLFTHKSFEARRPLKIALALIGSLSFQGSLIGWVADHRRHHMFTERDGDPHSPTRP
                     PEQPFGRLRGLFHAHIGWLFERASTSREKFAPDLLADRDIVLIDKLFVPCCVVTLALP
                     FGIGYAITGRFTGGLALFLWAGVLRVGLLHHVSWSTNSVCHVFGRRRFETDDSSKN"
BASE COUNT          345 a          741 c          726 g          394 t
ORIGIN      
        1 gcggcgggcc accagccggg gcctgccagg cccgtcccgt tgccgacgag ggaatcaacc
       61 aaccgtacgg cggcccgggc ggccatccgg gcatcgacct ccacgccagc tatggcgatc
      121 cgatccacgc ctgtcgcgcc gggacgatcg tcagcgccgg ctggatgggc ggctacggca
      181 acgccaccgt gatcgaccac ggtgggggca tggccacgct gtacggccat cagtcccaaa
      241 tcgtggtgag cgaaggccag cacgtcaacg ccggagacgt cattggctat gtcggaagca
      301 ccgggttctc gaccggcccc cacttgcact tcgaggtccg tatcaacggc aacacggtcg
      361 acccggcacc gtacctgtag gcggttcgcg tcggcgacga gcgtcaccgg agatggtgat
      421 ggtctcgatc gcggtgcgtc acgaggccgg gctgcgacga gggccagact ggccgcatga
      481 cgacgtggga atacatgatc ctgtcccttc cgccgtttgc ggcaccgacc cggcttccga
      541 cgagctcgcc ggcgttgcag gcactggacg aggtcggggc gcagggctgg gaggcagtgt
      601 ccatgaccgt cctcgcctcc ggtgaggtcg ccgtcctgct caagcggcca acccgcggat
      661 aggtcaagcc ccgagcggtt ggtgttggtc gtttggagta ccgtgagaag cggacggtcg
      721 ctgaaccagc cgacctaggt ggcaactgag ccgacctttg gcctccgcca agagagtctc
      781 cttcgaggac tccgcgttga cgagacgctc gccgagggtc agaggagcag tcgtgtaccg
      841 aatcgaccac gccgaccagg gctcgcggtc tacccgcgga ccctctcctg cagctcagcg
      901 cgcatgacgg tggccgaact ctccgccctc gtgaccctgg gactcggtgc agcagccctc
      961 gtgatcgtct gggtcgaggc gaaagtgccg gggttcagcg ggctgcggtt cgcgccggtg
     1021 cccatgccgc gacgccgagc acacgcggag aacgtgcacc ggtcgtgatc gaggcgagga
     1081 tgtcacacgg tggtgtcgcg ctgtacgact cctcgagcag ccgcctggtt cgatggctga
     1141 tgatgtcgtc ggggacaccg acgggcgtgg aggtcgacgg cgacgacgtg acggttacct
     1201 tcgccgatgg tgaacgatcg gtctatgacc tgaacaccgg cgtcctcgag cgctaggccg
     1261 gccggcctcc tgcctccggg agcgttccgg attgttcatc ggacggccca ccgatcgaga
     1321 ccggcccaag gcgagttcgg gccgagccgc cgctacgatg gcggtgttgc cccaaatccc
     1381 cggggcagac aatacggacg cgctgctcct gacctcggtg gtgcatggaa tcgcacccga
     1441 ggtggctatg cagatcctgg acgagcgtcg cactcctccc gctccttcgc gtcctccgcc
     1501 gccggccggt ccttccccaa cggtcgagac gatctggccg cagcggctgc tgacctcggt
     1561 gatcatcctc ggcccggtcg tcgcgttagg ggtgatcctc agccagatcc tcagcgacgg
     1621 gttggcttgg tacaacctcg tcatcgccgc gatcttctac ttcgtcgtcg gtcacggcgt
     1681 caccgtcggc ttccatcgtc tcttcaccca taagagcttc gaagcgcgtc gtccgctcaa
     1741 gatcgcgttg gcgctcatcg ggtccctgtc gttccaggga tcgctcatcg gatgggtcgc
     1801 cgaccatcgt cggcatcaca tgttcaccga gcgggacgga gatccacact ccccaacgcg
     1861 gccgcccgaa cagccctttg ggcgcctgcg cggcttgttc cacgcccaca tcggttggct
     1921 cttcgagcgc gccagcacgt cacgagagaa gttcgccccc gatctgctcg ccgatcgcga
     1981 catcgtcctc atcgacaagc tgttcgtgcc gtgctgtgtg gtgaccctcg ccttgccgtt
     2041 cgggatcggg tacgcgatca cgggacgctt taccggtgga ctggccctgt tcctctgggc
     2101 cggggtgctg cgagtcgggc ttctgcacca tgtgagctgg tcgacgaact cggtttgtca
     2161 tgtcttcggt cgacgccggt tcgagaccga cgactcgagc aagaac
//
LOCUS       DATCCH010000021         2025 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_251726, whole genome shotgun sequence.
ACCESSION   DATCCH010000021 DATCCH010000000
VERSION     DATCCH010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251726"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="VIJ44_00485"
     CDS             <1..469
                     /locus_tag="VIJ44_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY5164402.1"
                     /translation="DLKGVLRLVRRFSLGRGLLELGSERRAPLADDMGRRLITFALVG
                     VVSTTTSLALFLTLRHPLGSVGAVVVALGATAVGNSWAHRRWTLGRRGRAGLARHAIV
                     SAVIAVCGIGVSALGLVAVDAIGGGLVFELAALAVIWTAATLLRLWNLSKHTV"
     gene            609..>2025
                     /locus_tag="VIJ44_00490"
     CDS             609..>2025
                     /locus_tag="VIJ44_00490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY5164403.1"
                     /translation="MTQTRVVEPIPQPGGRTAISSRRVLVAVAALLIVAGATAVRVAD
                     LGSQPGGLYPDEAAEGVTASRILHDPGYHPLFIAEDAGREPLFAYVVAGAFAVFGEST
                     VTLRTVSAVIGILGVLAIGMALRRFGAGVALTAMAWAAGSLWLICVGRDGMRNILVPL
                     AGAIALAALLRWSDHPGRLSAVLAGAAIGAGLWTYQPLKLLPLLVLVWLWLLRRRNPS
                     AYRGLVADVRWCIGAFVVVGGPMMFVALTQPGNYFGRGAETSAFNGGNSQNGVVTHVI
                     RTLGQFMFVGDPNQRHDVNGIPLLGWPLFLLAVVGVVVAWRRRDQAVYLLLLLGLPVF
                     LLPPLVATEGSAPHFLRNLGLAPFVGAFVGLGAVATVRWLGRRGVRHLELMGGMVLAV
                     GLVVIGAGSAHAYFNRPVRDRYFAYSFAVVALANAANHGPRTAVVVDSYSAFDVQFLD
                     ARNPPTRFDPNTRIPMPGQYRQ"
BASE COUNT          260 a          661 c          692 g          412 t
ORIGIN      
        1 ggacctcaag ggcgtgctgc gactcgtccg gcggttttcg cttggccgcg gtctgctcga
       61 acttggttcg gagcgccggg cgcctctggc cgacgatatg ggccgacgac tcattacctt
      121 cgccctcgtc ggtgtcgtca gcacgaccac ctctcttgcc ctcttcttga ccctccggca
      181 cccgttgggt tccgtcggcg cagtcgtggt ggccctcgga gcgaccgcgg tgggcaactc
      241 atgggctcat cgccgctgga ctctcggacg acggggtcgg gccgggctcg cccgtcacgc
      301 gatcgtgtcg gccgtcatcg cggtatgcgg tatcggcgtg tcggcgttgg ggctcgtcgc
      361 ggtcgatgcc atcgggggcg gcttggtctt cgaactcgcg gcgctcgctg tcatctggac
      421 ggccgcgacg ctcctccgac tgtggaacct ctcgaaacac accgtatgac gacaagggct
      481 cccacggcgt gtcaccgacc gcgcaccgac acgattcgtt ctcgcctcat cccccaccta
      541 agcctgggat cgatgccaaa cccgagccgt cgcggagtgc cggaccgctc ggcggagacg
      601 tcggatcgat gacccagact cgcgttgtcg aaccaatccc gcaacccggc ggtcgcaccg
      661 cgatcagttc gcgacgggtg ctcgtcgccg tcgccgcatt gctcatcgtc gctggcgcca
      721 cagccgttcg cgtggccgat ctcggtagcc aacccggtgg cctttatccg gacgaagccg
      781 ccgaaggagt gaccgcgagt cggatcctgc acgaccccgg atatcacccg ctgttcatcg
      841 ccgaggacgc gggccgcgag ccgctgttcg cgtacgtggt cgcgggggca ttcgctgtct
      901 tcggcgagag cacggtgacc cttcggacgg tctccgcggt gatcggaatc ctcggggtgc
      961 tggcgatcgg gatggccctt cgacgattcg gggccggcgt ggcgctgacg gccatggcct
     1021 gggcggctgg ttccttgtgg ctcatctgcg tcggacgcga cgggatgcgg aacatcttgg
     1081 taccgctcgc gggagcgatc gcgctcgctg ccctgttgcg ctggtcggat catccgggtc
     1141 ggctgagcgc cgtgctcgcc ggcgcggcga tcggagcggg tctttggacg tatcagccac
     1201 tgaagttgct tcctctgctc gtgctggtgt ggttgtggct gctccgtcgg cgcaatccga
     1261 gcgcgtatcg aggcttggtc gcggacgtgc ggtggtgcat cggcgcgttt gtcgtcgtcg
     1321 gtgggcccat gatgttcgtg gcgttaaccc agcctgggaa ctacttcgga cggggcgccg
     1381 agacgagcgc cttcaatggc ggaaacagtc aaaacggcgt cgtgacccac gtcatccgaa
     1441 cgttgggaca gttcatgttc gtcggcgatc caaaccagcg ccacgacgtg aacgggatcc
     1501 cgcttcttgg gtggccgctc ttcttgctgg cggtcgtcgg ggtcgtcgtc gcgtggcgac
     1561 gcagggacca ggctgtgtat ctgctgctgc tcttggggtt gccggtgttc ttgctgccgc
     1621 cgctcgtggc cacagagggt tcggcacccc actttctccg gaaccttggc ctggctccgt
     1681 tcgtcggcgc ctttgtcggc ctcggcgccg tcgcgaccgt gcggtggctg gggcgacgcg
     1741 gtgtgagaca tctggagttg atgggcggga tggtgttggc tgtcgggctc gtcgtgatcg
     1801 gcgccggcag cgcccacgcg tacttcaacc gtccggtgcg tgatcgctat ttcgcgtatt
     1861 ccttcgcggt ggtcgcgctc gcgaacgctg ccaatcacgg accgcgcacc gcggtggtgg
     1921 tcgacagcta ctcggcgttc gacgtgcagt tcctcgacgc caggaatccc ccgacccgtt
     1981 tcgaccccaa tacccggatc ccgatgccag gtcagtatcg acaga
//
LOCUS       DATCCH010000022         4507 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694 k141_1491,
            whole genome shotgun sequence.
ACCESSION   DATCCH010000022 DATCCH010000000
VERSION     DATCCH010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4507
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1491"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1038
                     /locus_tag="VIJ44_00495"
     CDS             <1..1038
                     /locus_tag="VIJ44_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006834186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEY5164404.1"
                     /translation="LDGIIPPAGFTTVEELAANLGIENLRFATTVMMGGASPTAALQH
                     AATAVTTGIASTVLVTVGWNGYSIFRPKPDARRPRHTFLSTSVIDTAVDFYRPYGAMA
                     AVQMYAWLAMRYQQQYHVPDEATAAIALACRRHAQHNDKALMRGRELTLEQYRASRFI
                     SEPFRLFDCCLETDAAAAYIVTTEERARDLAHPPVVILGAAEGHPYPADDIPSRADPF
                     RIGLSYAAPEAFAQAGIRPDELDFLEVYDCFTHIVLLELEALGMCGPGEAADFVAGGT
                     IELGGRYPLNTHGGLLSQGHVWGLNHIVEATRQLRGEAGAAQVPDAEVGVVTGWGDFG
                     DGSLAILGRAR"
     gene            1035..1463
                     /locus_tag="VIJ44_00500"
     CDS             1035..1463
                     /locus_tag="VIJ44_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEY5164405.1"
                     /translation="MTEPSAAPKPRPVLEGMTAAYYGHCARGELRFQRCDECGAWRHP
                     PRVLCAACGSPRWSWQPSAGRGRIFTWTVVHQAMNPAFASEVPYSVIVVEMDEGVRVV
                     ANLRRAGTEVLRLDLPVEVIFEAVDGTLTLPMFQPVDEQR"
     gene            complement(1466..2272)
                     /locus_tag="VIJ44_00505"
     CDS             complement(1466..2272)
                     /locus_tag="VIJ44_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164406.1"
                     /translation="MNASERRYQRPDVPREDRQIRVGSLLFTMVEPHRGREVAYNRWY
                     ERDHFYAGCMVGKWQFAGARYVATRACKAKRYPADSPITPDPMVGSYLAVYWVLDGHH
                     DEWNEWGVEQVNWLHANERMFLDRDHIHTALYDYAAELNAPGSTMPVELALDRAYPGI
                     VAVIGEAGDGGVEAVTEWSRERGCPSDVMLVGTPLPLRGDRPADVPDQPTGNRFLHLH
                     FSAEDPLAVWEDRYASLGEDLAASGTGRVLFASPFLATVPGTDTYTDELW"
     gene            complement(2269..3774)
                     /locus_tag="VIJ44_00510"
     CDS             complement(2269..3774)
                     /locus_tag="VIJ44_00510"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY5164407.1"
                     /translation="MTPTPLVGDLLRAAVEAHPDREAYVHGEKRVTYAWLDRVADGFA
                     ATLLDLGVGRGDVVELLLGSSIKFAGCYLGALRVGAITSAINPRLGPVEQRSIFDRTE
                     PRVTVLGDGAAVPPGAASGQVISVDDLKDAFACDPPARFPRLDPTDPTCIVWTSGTTG
                     VPKGAVYDHETQAAISRNVGELTAAGDRRLVVLPFPHVGYMTRMWDELANATTIVLVG
                     EPWSAAETLRLVRDEGITMATGVPTQWTLVLDHPDVTGTDFSNLRVAAIGGAAIPPEL
                     VRRMRDTLGCPVITRYTSTEAGVTTSTLATDSPDVIATTVGRPTPEVELRISAPGDGA
                     ALGRGEVGEVLCRSSAMMRRYWRDPGLTDQVVDADGWLHTGDLGVVGDDGNLRLVGRL
                     KEMYIRGGYNVYPTEVESVLVEHPGVARAAVVGAPDPVLGEVGVAFVVAAPDADPTSV
                     SRVELRAWCRQRLADYKAPDRVIVVDDLPTTSMLKVDKRALSAQAEQGATR"
     gene            3895..4236
                     /locus_tag="VIJ44_00515"
     CDS             3895..4236
                     /locus_tag="VIJ44_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164408.1"
                     /translation="MASVTHLDKVRADIRTAWPSEIACPPLVVRWRAGSERAGWGTLV
                     QLARVRGEHRGNPIVESLTRAVSVELVAQSDVPETVEMSKHLIAGTLGLVGGAGRPEL
                     TAVDAYLSGAA"
     gene            4344..>4507
                     /locus_tag="VIJ44_00520"
     CDS             4344..>4507
                     /locus_tag="VIJ44_00520"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5164409.1"
                     /translation="MARFTDRVAVVTGAGSGLGRATAQRLAVEGALVAVLDIDEAAAK
                     DTAAQIEADGR"
BASE COUNT          692 a         1606 c         1545 g          664 t
ORIGIN      
        1 ctcgacggga tcatccctcc cgccgggttc accaccgtcg aggagctcgc ggccaacctt
       61 ggcatcgaga acctccggtt tgcgacgacc gtgatgatgg gcggcgccag cccgaccgcc
      121 gccttgcagc acgccgccac cgcggtgacg acgggcatcg cgtcgaccgt gctcgtcacc
      181 gtcggctgga acggctactc gatctttcgc cccaagcctg acgcccgccg gccccgccat
      241 acgttcctgt ccacgtcggt gatcgacacc gcagtcgatt tctaccggcc gtacggcgcg
      301 atggcggcag tgcagatgta cgcatggctc gccatgcgct accagcagca gtaccacgtg
      361 cccgacgaag ccaccgccgc gatcgcgctg gcctgtcgcc gccacgcgca acacaacgac
      421 aaggccctga tgcgcgggcg tgagctaaca ctcgagcagt accgggcgtc gcgcttcatc
      481 tccgagccgt tccgcctctt cgactgctgc ttggagaccg acgccgccgc ggcgtacatc
      541 gtcacgaccg aggagcgcgc ccgcgacctc gcccacccgc cggtcgtgat cctcggcgcc
      601 gccgagggtc acccgtaccc ggcggacgac atcccgagcc gcgctgaccc cttccggatc
      661 ggcttgtcgt acgcggcacc cgaggccttc gcgcaggccg gcatccgccc cgacgagctc
      721 gacttcctcg aggtctacga ctgcttcacg cacatcgtgc tgctcgagct ggaggccctg
      781 gggatgtgcg ggccgggcga ggcggccgac ttcgtcgccg gcgggacgat cgagctcggc
      841 ggccggtacc cgttgaacac gcacggcgga ctgctgtccc aaggccacgt ctggggtctg
      901 aaccacatcg tcgaggcgac ccgtcagctg cggggcgaag ccggcgcggc ccaggtcccc
      961 gacgccgaag tcggtgtcgt caccggctgg ggcgacttcg gcgacgggag cctcgcgatc
     1021 ctcggacggg cccggtgacc gagccgtccg cggcgccgaa gccccgtccg gtgctcgagg
     1081 ggatgaccgc ggcgtactac gggcactgtg cccgcggcga gctgcggttc cagcgctgcg
     1141 acgagtgcgg cgcgtggcgt cacccgcccc gggtcctgtg cgcggcctgc ggatccccgc
     1201 gatggagctg gcagccctca gccggtcggg ggcgcatctt cacctggacc gtcgtgcatc
     1261 aggcgatgaa cccggcgttc gcctccgagg tgccgtactc ggtgatcgtc gtggagatgg
     1321 acgaaggggt acgcgtcgtc gcgaacctac gccgcgccgg gacggaggtg ctgcgcctcg
     1381 acctcccggt cgaggtcatc ttcgaggccg tcgacggcac cctcacgctc ccgatgttcc
     1441 agccggtcga cgagcaacgc tgaggtcacc acagctcatc ggtgtaggtg tcggtgcccg
     1501 ggacggtcgc gaggaacggc gacgcgaaca ggactcgacc ggtgccgctc gccgccagat
     1561 cctccccgag cgaggcgtac cggtcttccc acaccgccag cgggtcctca gcggagaagt
     1621 gcaagtgcaa gaagcggttc ccggtcggct ggtcaggcac gtcggcgggc cggtcacctc
     1681 gtaacgggag aggagtcccg acaagcatga cgtccgacgg gcagccacgc tcccgcgacc
     1741 actccgtgac cgcctcgaca cccccgtcgc ccgcctcccc aatcaccgcg acgatgcccg
     1801 ggtaggcccg gtcgagggcc agctccaccg gcatggtgct gcccggggcg ttcagctccg
     1861 cggcgtagtc gtagagcgcg gtgtggatgt ggtcccggtc gaggaacatg cgctcgttcg
     1921 cgtggagcca gttcacctgt tcgacgcccc actcgttcca ctcgtcgtga tgaccgtcga
     1981 gcacccagta gacggcgaga tacgacccga ccatgggatc tggcgtgatc ggcgaatccg
     2041 cgggataccg cttcgccttg caggccctcg tcgcgacata ccgggcaccg gcgaactgcc
     2101 acttgccgac catgcatccg gcgtagaagt ggtcacgctc gtaccagcgg ttgtacgcga
     2161 cctcgcggcc ccgatggggc tcgaccatgg tgaacagcaa cgatccgacc cggatctggc
     2221 ggtcctcgcg gggcacgtcg ggccgctggt agcgacgttc agaggcgttc atcgtgtggc
     2281 tccttgttcc gcctgggccg acagggcccg cttgtcgacc ttgagcatgg acgtcgtggg
     2341 gagatcgtcc acgacgatca cccggtcggg cgccttgtag tcggccaggc gctgccggca
     2401 ccaggcccga agctctacgc gagacaccga ggttgggtcg gcgtcgggag cggccacgac
     2461 gaacgccaca ccgacctcac cgagaaccgg atccggggcg cccactaccg ccgctcgggc
     2521 cacgccggga tgctcgacga gcaccgactc cacctcggtc gggtacacgt tgtagccccc
     2581 acggatgtac atctccttga gccgcccaac gaggcgaaga ttgccgtcgt cgccgaccac
     2641 gccgagatca ccggtgtgga gccagccgtc ggcgtcgacg acctgatcgg tcaaccccgg
     2701 atcgcgccag tagcgccgca tcatcgccga cgaccggcac agcacctcgc cgacctcgcc
     2761 gcggccaagg gcagcgccgt ccccgggtgc gctgatccgc aactcgacct ccggtgtcgg
     2821 tcgtccgacc gtggtcgcga tcacgtccgg tgaatcggtg gcgagcgtcg acgtggtgac
     2881 cccggcctcg gtgctcgtgt atcgggtgat gactgggcag cccaacgtgt cgcgcatccg
     2941 gcgtaccagc tcgggtggga tcgcggcgcc accgatcgcg gcgactcgca gattcgagaa
     3001 gtcggttccg gtcacgtccg gatggtccaa caccagcgtc cactgggtcg gtacaccggt
     3061 tgccatcgtg attccctcgt cgcgcaccag gcgcagggtc tcggccgccg accacggctc
     3121 accgacgagg acgatggtgg tggcgttggc gagctcgtcc cacatgcggg tcatgtagcc
     3181 cacatgcggg aacgggagga ccacgagtcg gcggtctccc gccgcggtga gctcaccgac
     3241 gttgcgggag atcgccgcct gggtctcgtg gtcgtacacc gcccctttcg ggacaccagt
     3301 ggtgccactg gtccacacga tgcacgtcgg atcggtcgga tccaggcggg gaaaccgagc
     3361 gggcggatcg caggcgaacg catccttgag gtcgtcgacg ctgatcacct ggcccgacgc
     3421 cgcccccgga ggcaccgcgg caccgtcgcc gaggacggtc acgcgcggct cggtccggtc
     3481 gaagatgctg cgctgctcga cgggcccgag ccgggggttg atcgccgacg tgatcgcccc
     3541 cacccggagc gcgccgaggt agcagcccgc gaacttgatc gacgagccca acaggagctc
     3601 caccacatca ccccgaccga ccccgaggtc gagcagtgtc gcggcgaagc cgtcggcgac
     3661 gcggtcgagc caggcgtacg tcacccgctt ctcgccgtgg acatacgcct cgcggtcggg
     3721 gtgcgcttca accgctgcgc gcagtagatc acctaccaac ggcgtcggcg tcacggcgtc
     3781 ggactgtatt cgttgcgatc gagggtgacc cgggccatag ggtgagcgga tccgcgcccc
     3841 acgcgaggcg cggggttacc gtcggagcga cgcagacagg aggacaacga gcgcatggcc
     3901 tccgtcaccc acctcgacaa ggtccgggcg gacatccgca ccgcgtggcc atcggagatc
     3961 gcgtgtcctc cgctggtcgt ccgatggcgg gccgggtccg agcgggccgg atgggggacc
     4021 ctggttcagc tggctcgggt gcgaggtgaa caccggggca acccgatcgt cgagagcctc
     4081 acccgagcgg tctctgtgga gctcgtcgca cagagtgacg tgcccgaaac agtcgagatg
     4141 tccaaacacc tcatcgccgg gacgctcggt ctcgtcggag gagcgggtcg ccccgagctc
     4201 accgccgtcg acgcgtacct ctccggcgcg gcctgaaccc gggccccgag tcgcgagggc
     4261 gcccggcccg gggaccgcag ccggtcacct gacaccgcgt taggtttcgg gcgtcgtcgc
     4321 cggcaccgag caggggagag gtcatggcgc gcttcaccga tcgagtggcg gtggtcacag
     4381 gagcgggttc cggactcggc cgggcgaccg cccagcgtct cgccgtcgag ggggcgttgg
     4441 tcgccgtcct cgacattgac gaggccgcgg cgaaggacac tgcggctcag atcgaggccg
     4501 acgggcg
//
LOCUS       DATCCH010000023         2153 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_42976, whole genome shotgun sequence.
ACCESSION   DATCCH010000023 DATCCH010000000
VERSION     DATCCH010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42976"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..563)
                     /locus_tag="VIJ44_00525"
     CDS             complement(138..563)
                     /locus_tag="VIJ44_00525"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEY5164410.1"
                     /translation="MSQHALPNAVRCLIVGTALLGVAFVSVALGTAGATGPTPAPTKT
                     IKLGDNFYSPTKVTVTAGTVVTFKWTGSNTHNVTVLKGPEKFTSPNQSDGTYDRTFTK
                     PGTYKIVCTFHPGMNLTLKVKKAPAPTTTTSTTPPPSSS"
     gene            complement(635..1141)
                     /locus_tag="VIJ44_00530"
     CDS             complement(635..1141)
                     /locus_tag="VIJ44_00530"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF016040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050385 - ureidoglycolate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HEY5164411.1"
                     /translation="MPTTVVPRNAQPLTADAFAPLGWIPVTDTDPADGAQTLHFEWAD
                     PHLNVISHAATEIDHTERGAPLCTVLYRHATHTQALLVLNVDAVVACAPADVTFSRPS
                     DLDAIVAFRLHPLDTLVLHRGTWHWGPFPLGSEPVRLLNVQGRRYAEDNDRVDLLDQA
                     GAAIEVLA"
     gene            1215..1598
                     /locus_tag="VIJ44_00535"
     CDS             1215..1598
                     /locus_tag="VIJ44_00535"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY5164412.1"
                     /translation="MDVRSIEDVEPVVEHNGTVPVWWLVNPREMKEITDGGYLELVSE
                     FEVAGGGEVDPHSHPTHEFYYVTAGRGIMTISDEDHEIVQGDLVHIPPNAVHSLRPVS
                     AHAPIHCFCFAVGIRDAGEIDYTTH"
     gene            1601..>2153
                     /locus_tag="VIJ44_00540"
     CDS             1601..>2153
                     /locus_tag="VIJ44_00540"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5164413.1"
                     /translation="MGRVVVTRRLPEGGTDPLVTAGHEVVLNDEDVAFTKLQLCGAAR
                     DADALLGALTDRIDDDVLAAGGTRLRVVATVAVGYDNIDVSAAAARGIAVCNTPGVLD
                     DTTADLAFLLILAAARLASDAERDLRAGSWPGWGITQYLGVDVHGAILGVVGFGRIGQ
                     AVARRGAGFGMEVLHHTRSPTGMP"
BASE COUNT          316 a          694 c          804 g          339 t
ORIGIN      
        1 cgcccgacgc gacgaagccc cgcctggccg gtacctagcg agccgagcgg ggggcgcagc
       61 caacgtcacc ggcttctgtc agggggtggt gcgctctgcc ggtccctaga ccgccgcggc
      121 gcgctcgcct tcgacggcta cgacgacgac ggcggcggtg tcgtgctggt ggtcgtcgtc
      181 ggcgctggcg ccttcttgac cttgagggtc agattcatgc cggggtggaa ggtgcagacg
      241 atcttgtagg taccgggctt cgtgaacgtt cggtcgtacg tcccgtcgct ctggttggga
      301 gaggtgaact tctccggacc tttgagaacg gtgacgttgt gggtgttcga acccgtccac
      361 ttgaaggtca cgacggtgcc ggcggtcacg gtcactttcg tcgggctgta gaagttgtcg
      421 ccgagcttga tcgttttggt cggggccggt gtcgggccag tcgcgccggc ggtcccgagg
      481 gcgacgctca cgaaggccac gccgagcagg gccgtaccga cgatcaagca gcggacagcg
      541 ttggggagag cgtgctgcga catgcgctca cggtatcggc cgatcccctg cggcgaagca
      601 tcagcctctc ggcacgcggc gcggcgcggt gtcgtcaggc gagcacctcg atggccgcgc
      661 cggcctggtc gaggaggtcg acacggtcgt tgtcctcggc gtagcgccga ccctgcacgt
      721 tcaagagccg aaccggctcg gacccgagcg ggaagggacc ccaatgccaa gtcccccggt
      781 ggaggaccaa cgtgtcgagg gggtggagcc ggaacgcgac gatggcgtcg aggtcgctcg
      841 gtcgagagaa cgtgacgtcg gccggggcgc aggcgacgac cgcgtcgacg ttgaggacga
      901 ggagcgcttg ggtgtgggtg gcgtgccggt acaggacggt gcagagcggc gcgccgcgtt
      961 cggtgtggtc gatctcggtg gcggcgtggc tgatgacgtt caggtgcgga tcggcccact
     1021 cgaagtgcaa ggtctgggcg ccgtcggcag ggtcggtgtc ggtgacgggg atccacccga
     1081 gcggtgcgaa cgcgtcggcg gtgagcggct gggcgttgcg gggcacgacc gtggtcggca
     1141 tcgccgcagt gtaggggtga gcccccgggc cgctcggggg gcgccccggg gtcgaggcct
     1201 ctacgcttcg ggccatggac gtgcgctcta tcgaggatgt cgagccggtc gtcgagcaca
     1261 acgggacggt ccccgtgtgg tggctcgtga acccgcgcga gatgaaggag atcaccgacg
     1321 ggggctacct ggagctcgtc agcgagttcg aggtcgccgg cgggggcgag gtcgacccgc
     1381 actcgcaccc gacccacgag ttctattacg tcacggccgg gcgcgggatc atgaccatct
     1441 ccgacgagga tcacgagatc gtgcaaggcg atctcgtgca catcccgccc aacgcggtgc
     1501 acagcctccg cccggtcagt gcccacgcgc ccatccactg cttttgcttc gcggtcggca
     1561 tcagagacgc cggcgagatc gactacacga ctcactgagc gtgggccgcg tcgtcgtcac
     1621 ccgacggctc cccgagggcg ggaccgaccc gctcgtcacc gccggtcacg aggtcgtcct
     1681 caacgacgag gacgttgcct tcacgaaact gcagctgtgc ggcgcggcac gtgacgccga
     1741 cgcgctcctg ggcgcgctca ccgaccggat cgacgacgac gtcctggccg caggcggaac
     1801 ccggctccgg gtcgtcgcca ccgtcgcggt cggctacgac aacatcgacg tctccgccgc
     1861 cgcggctcga gggatcgcgg tgtgcaacac accgggggtg ctcgacgaca ccaccgccga
     1921 cctcgcgttc ctgttgatcc tcgcagccgc tcgactcgcg tcagatgccg aacgcgacct
     1981 gcgcgcgggc agctggccgg gatgggggat cacccagtat ctcggcgtcg acgtgcacgg
     2041 tgcgatcttg ggtgtcgtgg gcttcgggcg gatcggacag gccgtcgccc gccggggcgc
     2101 aggcttcggc atggaggtgc tgcaccacac ccggagccca accgggatgc ccg
//
LOCUS       DATCCH010000024         4187 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_293410, whole genome shotgun sequence.
ACCESSION   DATCCH010000024 DATCCH010000000
VERSION     DATCCH010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4187
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293410"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1560)
                     /gene="murJ"
                     /locus_tag="VIJ44_00545"
     CDS             complement(<1..1560)
                     /gene="murJ"
                     /locus_tag="VIJ44_00545"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEY5164414.1"
                     /translation="MSPRGRGRGVRPHDTGRLLRSSVVVGVGTALSRVTGLLWRAAAA
                     YAIGITALSGTYAFANETPNMLYELIVGGVLTATLVPQFVRHFQDDDEEATSAVVTVS
                     MLLLVGVAILGVLLAPFVADLLTLRVTGSQQGSQRRLATDLLRLFMPQIVFYGFTALA
                     TALLQARRRFAAAAFAPILNNVVLIGLFLALPRLAGRPITVARVLNDTPLLLFLGLGT
                     TAGIAAMALALVPALRGARVHLRFLAAWRHRAVGIVVRLSGWTIGYVIANQIALWVVL
                     VLANGQPGGPAAYLLAYTLFQLPHGLFAVSIMTTAGPELAAAARDLPALRHRFARALR
                     LVLTVVIPSAALAVALARPVVVALLQRGAFSGSDSALVAHTLVGFAVGLPFFSVYLFS
                     LRAFYSLEDTRTPFVINCVENAVNIGLALAFIGPFGIAGLGYAFSGAYAVAAVVTLGV
                     LSRRIGGLQGRGIETSFIKVGVASAAAGTAAWFAAGAIGWAGGTRAVGATVVGAAVGL
                     AGIAAGLSLTGV"
     gene            complement(1557..3587)
                     /locus_tag="VIJ44_00550"
     CDS             complement(1557..3587)
                     /locus_tag="VIJ44_00550"
                     /inference="COORDINATES: protein motif:HMM:NF039636.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6049 family protein"
                     /protein_id="HEY5164415.1"
                     /translation="MSRRLTAGFAALTAALALAAAVAPPGRAATPNDPSRLALVAQDA
                     YTPLGGIFHAQVQIGTSGTPGMQLSVIAHQAIQTRSAFGDTVTDKALGGVLAQVLLPV
                     DALGVNPDGSRPLAIGLEIPGGAFDPARLAVHLAGVYPLEMELRDAHDQPLARFVTYL
                     VAVDVGASGQAQPLTARLGVSWVWPLDAHPSGRPDGTTDPTVAAALEPSGRLGRQGAA
                     LQRHPTVPVVLAPTPETLDTWGGLSLARGDPAAASGAATLRGLLGSSRAQVLTGPYVS
                     LDLPSVLHSGLGGALDAEFVQGGVAVDKFFGTHVDARTALAQPVDADALGRLRARGVD
                     RVVVDEAAVGAQNDRLTVTAPFSLEPAPNLAPAGPVAAVSNDTRLAQLLNGDGSPALR
                     AQRFLAGLSLTALETPAATRAVVVVNPSGFDAPAALLDAVLTGLTNHPWLNPMTLDTV
                     FTTVTPSSSNTVRPLAPYPAPAAPVSAKSYGDTETRLAAFGTLVGPTDVRVGRAERLL
                     LSSLSADWSGPVGATQARSELVGVNAIIDDFLTRIRVPAPGTITLTARSGAIPITFRN
                     DTGQPVRVLVSLRSNKLDFPQGSVQTFALPTRSVTVRFDVRSRTSGTFPLEMSVRSAD
                     GALRIADGRFKVRSTVVSTVGVALAAGAAFFLAAWWIFEIRRRRRARAGSGA"
     gene            complement(3584..>4187)
                     /locus_tag="VIJ44_00555"
     CDS             complement(3584..>4187)
                     /locus_tag="VIJ44_00555"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5164416.1"
                     /translation="SAAAVAECVARLRRDAYGSVVTSPLTETAAAPFLAAGFVHREHL
                     HLLAHSLESVARFDADRPPVRRAWRGDRAAVLDIDAHAFMPSWQLGAAGLREALRATP
                     SVRFRVGHHPGDPFPVAYAVTGRAGHHGYLQRLAVHPSERGRGHGRALIDDGLRWVRR
                     GGASRCLVNTQENNHDALALYERCGFERLPTGLAVLSADL"
BASE COUNT          688 a         1478 c         1539 g          482 t
ORIGIN      
        1 gaccccggtg agactgagcc cggccgcgat cccggcgagc ccgacggcgg cgccgaccac
       61 ggtcgccccc acggccctgg tcccgccggc ccagccgatc gctcccgcgg cgaaccacgc
      121 cgcggtcccg gcggccgcgc tcgcgacgcc gactttgatg aagctcgtct cgatgccccg
      181 cccctggaga ccaccgatcc gacggctcag caccccgagg gtcacgacgg cggcgacggc
      241 gtaggcgccc gagaacgcgt agccgagccc ggcgatgccg aacggcccga tgaacgccag
      301 ggccaatccg atgttcaccg cgttctccac gcagttgatc acgaaggggg tgcgggtgtc
      361 ctccagcgag tagaaggcgc gcagcgagaa gaggtacacc gagaagaacg gcaatccgac
      421 ggcgaagccg accagggtgt gcgcgacgag cgccgagtcg ctacccgaga acgcgccgcg
      481 ttggagcaac gcgaccacga ccggccgcgc caacgcgacg gcgagcgccg ccgatgggat
      541 gacgacggtc agcacgagcc gcaacgcgcg cgcgaaccgg tggcgcagcg ccggaaggtc
      601 acgggccgcc gccgcgagct cagggccggc ggtcgtcatg atcgacaccg cgaacaatcc
      661 gtgtgggagc tggaagagcg tgtacgcgag cagatacgcc gcgggaccgc ccggctggcc
      721 gttggcgagc accagcacga cccagagcgc gatctggttc gcgatcacat acccgatcgt
      781 ccaaccggac aagcgcacca cgatccccac cgcccggtga cgccacgcgg ccaagaatcg
      841 gaggtgcacc cgcgccccgc gcagcgccgg caccagggcg agtgccatcg cggcgatgcc
      901 ggcggtggtt ccgagtccga ggaacaacag cagcggcgtg tcgttcagga cccgggccac
      961 cgtgatcggg cgtcccgcga gccgaggaag cgcgaggaac agcccgatga ggaccacgtt
     1021 gttcaggatc ggtgcgaagg ctgcggccgc gaaccgtcgc cgcgcctgca ggagggcggt
     1081 cgcgagggcc gtgaagccgt agaacacgat ctggggcatg aagagccgca gcagatcggt
     1141 cgccagtcgc cgttgcgacc cttgctggga ccccgtcact cgcaaggtca gtaggtcggc
     1201 gacgaacggg gcgagcagca ccccgaggat cgccacgccg accagcaaca gcatcgagac
     1261 cgtgacgacc gcgctggtgg cctcctcgtc atcgtcttgg aagtggcgga cgaactgagg
     1321 gacgagggtg gcggtcagca ccccgccgac gatcagctcg tagagcatgt tcggggtctc
     1381 gttcgcgaac gcgtacgttc ccgacagcgc ggtgatcccg atcgcgtacg ccgcagccgc
     1441 gcgccacaac aaacccgtca cccgggacag cgcggtgccg accccgacga cgacgctcga
     1501 tcggaggagg cggcccgtgt cgtgaggacg gacgccgcgc ccgcgcccgc gtgggctcac
     1561 gcgccggacc ctgcccgtgc ccgccgccgc cggcggatct cgaagatcca ccacgcggcg
     1621 agaaagaacg ccgcgcccgc cgccagtgcg acaccgaccg tgctcaccac cgtcgaccga
     1681 accttgaacc ggccgtcggc gatgcgcagc gccccgtcag ccgagcggac gctcatttcg
     1741 agcgggaacg tccccgacgt tcgtgagcgc acgtcgaagc gcaccgtcac gctccgcgtc
     1801 gggagcgcga acgtctgcac cgaaccttgt gggaagtcga gcttgttgct gcggagggac
     1861 acgagcaccc ggacaggctg accggtgtcg ttccggaacg tgatcggaat tgcgcccgag
     1921 cgggccgtca acgtgatcgt gccgggagcc ggcacccgga ttcgggtcaa gaagtcgtcg
     1981 atgatggcgt tgacgccgac cagctcgctg cgagcctggg tcgcgcccac ggggcccgac
     2041 cagtccgccg acaacgagga caaaagcagc cgctcggcgc gacccacgcg aacgtcagtg
     2101 ggtccgacca gcgttccgaa cgccgcgagt cgggtctcgg tgtcgccgta ggacttggcg
     2161 gacaccggcg cggccggagc ggggtacggc gccaggggac ggacggtgtt actcgagctc
     2221 ggcgtgaccg tcgtgaacac cgtgtcgagg gtcatcgggt tgagccaggg gtggttggtc
     2281 agcccggtga gcacggcgtc gaggagcgca gcgggtgcgt cgaagccgct cgggttgacg
     2341 acgaccacgg cccgggtcgc cgccggggtc tccaacgcgg tcaacgacaa gcccgcgagg
     2401 aaccgctggg cccgcaacgc cggtgagccg tcaccgttca gcagctgcgc caggcgggtg
     2461 tcgttcgaga ccgcggccac cggcccggcc ggcgccaagt tgggtgccgg ctcgagcgag
     2521 aacggggccg tgacggtcag ccggtcgttc tgggcgccga ccgcggcctc gtcgaccacc
     2581 acccggtcga cgccgcgggc ccggagccgt cccaacgcgt cagcgtcgac cggctgcgcc
     2641 aacgcggtcc gcgcatcgac gtgggtccca aagaacttgt cgaccgcgac cccaccctgg
     2701 acgaactccg cgtccagcgc tcctccgagc ccggaatgca agaccgacgg caagtcgagc
     2761 gagacgtacg ggccggtgag cacctgagcc cggctcgagc cgagcaaacc acgcagcgtc
     2821 gccgcgccgc tcgcggcggc gggatcccca cgggcgaggc tgaggcctcc ccaggtgtcg
     2881 agcgtctcgg gggtcggcgc caacaccacg ggcaccgtcg ggtggcgttg gagggcggcg
     2941 ccctggcgac cgagccggcc cgaaggctcg agcgccgcgg ccaccgtcgg gtcggtggtc
     3001 ccgtcggggc ggcccgacgg gtgagcgtcg aggggccaga cccacgagac gccgagccgc
     3061 gccgtgagcg gctgcgcctg gccggacgca ccgacgtcca ccgcgacgag gtacgtcacg
     3121 aaacgggcga ggggctggtc gtgcgcgtcg cgcagttcca tctccagcgg gtacacgccg
     3181 gcgagatgca ccgcgagccg cgccggatcg aacgcgccgc cagggatctc gaggccgatc
     3241 gcgagcgggc ggctcccgtc ggggttcacg ccgagggcat cgaccgggag gaggacctgg
     3301 gccaggacgc cccccaaggc tttatcggta accgtgtcac cgaacgccga ccgcgtctgg
     3361 atcgcctgat gggcgatgac actcagctgc atcccgggcg tcccggacgt cccgatctgc
     3421 acctgggcgt ggaagatccc accgagcggg gtgtaggcgt cctgggccac gagcgcgagt
     3481 cggctcggat cgtttggtgt cgcggctcgc ccggggggcg cgaccgccgc ggcgagcgcc
     3541 agcgccgcgg tgagcgcggc gaatcctgct gtgaggcgac gactcacagg tcggcgctga
     3601 gcacggccag cccggtgggg agccgctcga atccgcagcg ctcgtagagc gcgagcgcgt
     3661 cgtggttgtt ctcctgggtg ttgaccaggc accgggaagc gccaccgcgg cgaacccatc
     3721 gcagaccgtc gtcgatgagc gcccggccgt gtccccgacc gcgttccgat gggtgcaccg
     3781 cgagccgttg gaggtagccg tgatgcccgg cccgtcccgt gacggcgtac gccaccggga
     3841 acggatctcc ggggtggtga ccgacccgga accggaccga cggggtggcc cggagggcct
     3901 ctcggagccc cgccgcgccg agctgccagg acggcatgaa cgcgtgggcg tcgatgtcga
     3961 gcaccgccgc ccgatcgccg cgccatgccc ggcggaccgg gggtcgatcg gcgtcgaacc
     4021 gggcgaccga ctccagcgag tgggcgagaa gatggaggtg ctcccggtgc acaaacccgg
     4081 cggcgaggaa cggagccgcc gcggtctcgg tgagcgggct cgtgactacc gacccgtagg
     4141 cgtcgcggcg cagtcgggcg acgcattcgg cgaccgcggc ggcgctc
//
LOCUS       DATCCH010000025         2365 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_251996, whole genome shotgun sequence.
ACCESSION   DATCCH010000025 DATCCH010000000
VERSION     DATCCH010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251996"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2263
                     /locus_tag="VIJ44_00560"
     CDS             <1..2263
                     /locus_tag="VIJ44_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011873266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEY5164417.1"
                     /translation="RVEDPRILRGDAKYVDDLSPAGTAHLAFVRSTMAHARITAVDTT
                     DAEAMPGVIGVHTKDTLSLAPVQGFVMLPPVFSRPPLADGVVRFVGDIVAVVVAETRA
                     QAVDASELVVVDYDPLPTLVDVEAALEPDAPQLFPDHGSNVAIEFNFGEDPTVLDGAD
                     VVVQGRFVNQRVAPAPMETNGVLIEPTPDGGLLATVPTQGPFGVRDPLAAALGLEPDQ
                     VRVVAPAVGGGFGAKTGAYCEYIIAATLARDLGRPVKWTETRSENMVAMTHGRGQLQL
                     IEMGLRRDGTIVGVKGRVVCDGGAYPAIGAFLPFLTRTMAQGVYAIPKVELNSVSVAT
                     NTTPTAAYRGAGRPEATAFLERIIDMAADELEIDPVEIRKRNFLPPEAFPLTTVTGAN
                     YDVGEYAKALDEACRVAGYEQLRAEQQAQRERGGVHQLGIGVSAYVEITAGGLFQEFG
                     GVEIQADGTVLARVGTSAHGQGHETAFAMIVAELLDVAMDRVRLVQSDTALVPRGSGT
                     MGSRSLQTAGSALYRASEEVIEKAKRLAAHLLEASPDDIVLHDGGKVGVAGVPATALS
                     WAELATALEDPDRRPDDWEGALASELDFNQGEATYPFGAHIAVVDVDTETGRVELLRH
                     VAVDDCGRILNPLLVAGQQHGGIAQGVAQALYEHMIYDEDGNPLTGNLMDYAFPSAAE
                     FPSFEASNTETPTPLNPLGAKGIGESGTIGSMPAVQNAVVDALSHLGVRHLDMPLGAE
                     RVWRAIQDARAGT"
BASE COUNT          380 a          843 c          796 g          346 t
ORIGIN      
        1 gcgcgtcgaa gacccgcgca tcctgcgggg cgacgccaag tacgtcgacg acttgtcgcc
       61 ggccgggacg gcccacctcg cgtttgtccg ctcgacgatg gctcatgccc gcatcacggc
      121 cgtcgacacc accgacgccg aagccatgcc gggcgtgatt ggcgtgcaca ccaaggacac
      181 gttgtcgttg gcgccggtcc aggggttcgt catgctcccc ccggtcttca gccgtccacc
      241 actcgctgac ggcgtcgtgc gcttcgtcgg cgacatcgtc gcggtggtcg tcgccgagac
      301 ccgggcccag gctgtcgacg cctcggagct ggtcgtcgtc gactacgacc cgctcccgac
      361 gctcgtggat gtcgaggcgg ccctcgaacc cgacgcgccg cagctcttcc ctgaccacgg
      421 atcgaacgtc gcgatcgaat tcaacttcgg cgaggacccg acggtcctcg acggtgccga
      481 cgtcgtcgtg cagggccggt tcgtcaacca gcgggtcgct ccggcgccga tggagaccaa
      541 cggcgtcctc atcgagccca cgccggacgg cggtctgctc gcgaccgtgc ccacccaggg
      601 gccgttcggt gtccgagatc cgctcgccgc cgcgctcggc ctcgagcccg accaggtccg
      661 cgtcgtggcc ccagccgtcg gtggcgggtt cggcgccaaa accggggcgt actgcgaata
      721 catcatcgcc gccaccctcg cccgggacct cggtcggccg gtgaaatgga ccgagacccg
      781 ctccgagaac atggtcgcga tgacccacgg gcggggacag ctccagctga tcgagatggg
      841 actacgccgt gacgggacca ttgtcggcgt caagggtcgc gtcgtgtgcg acgggggcgc
      901 ctacccggcg atcggcgcgt tcctcccctt cttgacccgc accatggcgc aaggcgtcta
      961 cgcgatcccg aaggtggagc tcaactcggt cagcgtcgcg acaaacacca ccccgaccgc
     1021 ggcctaccgc ggcgcgggac gcccggaggc caccgccttc ttggagcgga tcatcgacat
     1081 ggcggccgac gagctcgaga tcgatccggt cgagatccga aagcggaact tcctcccgcc
     1141 cgaagcgttc ccgctgacca ccgtcaccgg cgcgaactac gacgtcggcg agtacgcgaa
     1201 ggcgctcgac gaggcgtgcc gggtcgccgg gtacgagcag ctgcgagcgg aacagcaagc
     1261 gcagcgcgag cgcggcggcg tccatcagct cggtatcggc gtctcggcgt acgtcgagat
     1321 caccgccggt ggccttttcc aggagttcgg tggcgtggag atccaagccg acggcaccgt
     1381 gctcgcccgg gtcgggacgt cggctcatgg gcaaggccat gagaccgcgt tcgcgatgat
     1441 cgtcgccgag ctgctcgatg tcgcgatgga tcgggtccga ctggtgcaat ccgacactgc
     1501 cctcgtgccc cgaggaagcg gcacgatggg ttcgcgctcg ctgcagaccg cggggagcgc
     1561 cctgtaccgg gccagtgaag aggtgatcga gaaggcgaag cgcctcgcgg cgcatctgct
     1621 cgaggccagt cccgacgaca tcgtcctcca cgacggcggg aaggtcggcg tcgccggcgt
     1681 gccggccacc gcgctcagct gggctgagct cgccaccgca ctcgaggatc cggaccgccg
     1741 ccccgacgac tgggagggtg cgcttgccag cgagctcgac ttcaaccagg gcgaggcgac
     1801 ctatccgttc ggggcgcaca tcgcggtcgt ggacgtcgac accgagaccg gtcgagtcga
     1861 gctgctccgt cacgtcgcag tggacgactg cggccgaatc ctgaacccgc tcctcgtcgc
     1921 cggacagcag cacggcggga tcgcccaggg tgttgcccaa gccctctacg agcacatgat
     1981 ctacgacgaa gatgggaacc cgttgaccgg gaacctcatg gactacgcct tcccgagcgc
     2041 cgccgagttc cccagcttcg aagcgtcgaa caccgagacg cccaccccac tgaacccgtt
     2101 gggagcgaag ggcatcggcg agtcgggcac gatcgggtcg atgcccgcgg tgcagaacgc
     2161 cgtcgtcgac gcgctgagcc atctcggggt ccggcacctt gacatgccac tgggggcgga
     2221 gcgggtctgg cgcgccatcc aggacgcgcg tgccggaacc tgagggcacg ttcactccgc
     2281 caacgctgcg cctcaccagc gcgcagtacc gggacatcgt cgcccattgc tacgacggcc
     2341 taccagacga ggcttgcggc ctcct
//
LOCUS       DATCCH010000026         4530 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_86440, whole genome shotgun sequence.
ACCESSION   DATCCH010000026 DATCCH010000000
VERSION     DATCCH010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4530
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86440"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1356)
                     /gene="sufB"
                     /locus_tag="VIJ44_00565"
     CDS             complement(<1..1356)
                     /gene="sufB"
                     /locus_tag="VIJ44_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEY5164418.1"
                     /translation="MAVLGIDLGKYKLGWADSTADYVYTPKKGLNEDVVRDISGYKSE
                     PDWMTKFRLNALKRFERKPMLAWFAKNMPDIDFDDIYYYLKPTEGQVADWDELPDEIK
                     ATYEKLGIPEAERKFLAGVTAQYESETVYHKNRDDLAKQGVIFTDMDTALREYPEIVQ
                     RYLGTVIPPGDNKFAALNSAVWSGGSFVYVPEGTHVEMPLQAYFRINSENAGQFERTL
                     IIAEEGASVHYVEGCSAPVYTTDSLHSAVVELIAKPGSRIRYTTIQNWSPNVYNLVTK
                     RARAETESTVEWIDGNLGSKLTMKYPAVVMVGPKAHGEVLSVAYAGPGQHQDAGAKMT
                     HAAPETTSIIDSKSISKGGGRTTYRGLVKVDEGAHHVKSHVRCDALILDDESRSDTYP
                     YMEIEEKDARIGHEATVSKVGDDQLFYLMSRGLTEQQATAMVVNGFIEPIIKALPMEY
                     AV"
     gene            complement(1477..4398)
                     /locus_tag="VIJ44_00570"
     CDS             complement(1477..4398)
                     /locus_tag="VIJ44_00570"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HEY5164419.1"
                     /translation="MRVPTMERRSFRLRAWIIGFVVVLVILLFSLRGLAGFYTDYLWF
                     DSLGQGGTWSALLGAKVVPAVVFTAVFFVIMLASLLIADRLAPKVHPMGPASPEDELV
                     NRYQQVAGRYQGRIRVVIAAFFALIAGIGVSSQWRNWVLFMHRVDFGVKDPQFHKDVG
                     FYVFQLPFIQFILDWLFAGLVIVLLVTAVAHYLNGGIRFQSPLQRVTPQVKAHLSVVV
                     AVMALVKTAQYYFSRFGLTLSGRGVVDGATYTDVKAQLPAFNLLIFISVVAAGLFLWN
                     IFRRGWVLPVIAVGLWGFVSIVVGTIYPAAIQNFRVKPNELAQERPYIGRNITATRAA
                     FKLNNVTVNPFNYTANLNPSVVDTNRGTIENARLWDPAVIRSTYQTLQGLQPYYSINN
                     VDIDRYTINGQTSQVLVAARDLNSNSLPSQSWVNQHVVYTHGYGSIVSPTNQATSSGD
                     PAFTLSNIPPTSNGIPLTNQGAQLYFGENQSGYVIVNAKQDEFNYAQANNTNASTRYK
                     GRDGVKLSNIIRKAAFALRFGDINPLISGQVTGSSKLLMVRDIQSRVQKLAPFLQFDS
                     DPYPVVVNGRITWVLDAYTTSAQYPYSQTYSGDGGLSTSFNYVRNSVKATIDAYDGTV
                     HFYVIDPKDPMVRSYREAFPDLFSDFSKMPAAMRDHLRYPENLFKLQSDVFSTYHVTN
                     TSAFYAGTERWLLSPDPNSVLGGTVTPTTTRGTQRAPTITATTPRQDPYYLYIRLPGD
                     QRENFLILQPFVPVSQNNQQTRLASFMTAKSDPASYGQLQAFVMPQGGNVNGPVQVAN
                     NIQSDQALSQQFTLLSQGGSAIVKGNIQLIPVGNSIVYVQPIFVQQGSSQGYPQFRFV
                     VVFTQGKNPTFAATVSDALTKLFGGATTPTTPTGPTTPTTPTPGTTNPTAQNLLNQAA
                     QKFTDADTALRNGDLATYQSDIAQGESLVKQAQAILSGGSTATTTPLTSASAAAFHGL
                     P"
BASE COUNT          722 a         1295 c         1683 g          830 t
ORIGIN      
        1 gacggcgtac tccatcggga gggccttgat gatcggttcg atgaagccgt tcacgaccat
       61 cgccgtcgct tgctgctcgg taaggccccg gctcatcagg tagaagagct ggtcgtcacc
      121 gaccttcgag acggtcgcct cgtggccgat gcgggcgtct ttctcttcga tctccatgta
      181 cgggtaggtg tcggagcggc tctcgtcgtc gaggatgagc gcgtcgcagc gcacgtggct
      241 cttgacgtgg tgggcgccct cgtcgacctt caccaggccg cggtacgtcg tgcggccgcc
      301 acccttcgag atcgacttcg agtcgatgat cgacgtcgtc tccggcgcgg cgtgggtcat
      361 cttggcgccg gcgtcctgat gctggccggg accggcgtag gcgaccgaca ggacctcacc
      421 gtgggccttc gggccgacca tcacgacggc cgggtacttc atcgtgagct tggagccgag
      481 gttgccgtcg atccactcga cggtcgattc ggtctcggcg cgggcgcgtt tcgtcaccag
      541 gttgtagacg ttcggcgacc agttctggat cgtggtgtag cggatccgcg agccgggctt
      601 ggcgatgagc tcgacgacgg cggagtgcag cgagtcggtc gtgtagaccg gcgccgagca
      661 gccctcgacg taatgcaccg acgcgccttc ttcggcgatg atgagcgtcc gctcgaactg
      721 gccggcgttc tcagagttga tccggaagta cgcctggagc ggcatctcga catgggtgcc
      781 ctcggggacg tacacgaagc tgccgccgct ccacaccgcg gagttcaacg ccgcgaactt
      841 gttgtcgccg ggcgggatga cggtgccgag gtatcgctgc acgatctcgg ggtactcgcg
      901 cagcgcggtg tccatgtcgg tgaagatgac accctgcttg gcgaggtcgt cgcggttctt
      961 gtggtagacg gtctcggact cgtactgggc ggtgacaccg gcgaggaact tccgctccgc
     1021 ttcggggatg ccgagcttct cgtacgtggc cttgatctcg tcggggagct cgtcccaatc
     1081 ggcgacctga ccctcggtcg gcttgaggta gtagtagatg tcgtcgaagt cgatgtcggg
     1141 catgttcttc gcgaaccagg ccagcatcgg cttgcgctcg aagcgcttca gggcgttgag
     1201 ccggaacttg gtcatccagt ccggctcgga cttgtagccc gagatgtcgc ggacgacgtc
     1261 ctcgttcagg cccttcttgg gggtgtagac gtagtccgcg gtgctgtcgg cccagccgag
     1321 cttgtacttg ccgagatcga tgcctaagac ggccatgagg acgggctccc gtcgccgagg
     1381 gcgatccggg cccggcgggg ggtgccggac cggggggttt ccactacgcc gatagtaaca
     1441 attaccgggg ggtcgaacaa tcccgggggc ccggcgtcag ggaaggccgt ggaaggcggc
     1501 ggcgctcgcc gaggtgaggg gcgtggtggt cgcagttgag ccgccgctca ggatcgcctg
     1561 agcctgcttg acgaggctct cgccttgggc gatgtcggac tggtaggtgg cgaggtcacc
     1621 gttgcgcagc gccgtgtcgg cgtcggtgaa cttctgggcg gcctggttga gcaggttctg
     1681 ggccgtcggg ttggtggtcc ccggggtcgg ggtcgtcggg gtggtcggcc ccgtcggggt
     1741 ggtcggggtc gtcgccccac cgaagagctt ggtgagcgcg tcgctgacgg tggcggcgaa
     1801 cgtcgggttc ttgccctggg tgaagacgac cacgaaccgg aactgcgggt agccctgcga
     1861 ggagccctgc tgaacgaaga tcggctgcac gtagacgatc gagttgccga ccgggatgag
     1921 ctggatgttg cccttcacga tcgccgagcc gccctggctg aggagggtga actgctggga
     1981 cagcgcttgg tcactctgga tgttgttggc cacttggacc ggcccgttga cgttgccgcc
     2041 ttggggcatc acgaacgcct ggagctgccc gtagctcgcg gggtcggact tggcggtcat
     2101 gaacgaagcg aggcgggtct gctggttgtt ctgggagaca ggcacgaagg gctggaggat
     2161 caggaagttc tcgcgttgat cgccgggcag ccggatgtag aggtagtacg gatcctggcg
     2221 gggagtcgtg gcggtgatgg tcggcgcccg ctgggtgccc cgggtcgtgg tcggggtcac
     2281 ggtgccgccg agcaccgagt tcgggtcggg ggagagcagc cagcgctcgg tgccggcata
     2341 gaaggcgctg gtgttcgtca cgtggtacgt ggagaagacg tcggattgga gcttgaagag
     2401 gttctcgggg taccggaggt gatctcgcat cgcggccggc atcttcgaga agtcgctgaa
     2461 gaggtcgggg aacgcctccc gataggagcg gaccatcggg tccttggggt cgatgacgta
     2521 gaagtggacg gtcccgtcgt aggcgtcgat ggtggccttc accgagttac gcacgtagtt
     2581 gaaactggtg gagagcccgc cgtcgccgct gtaggtctgg gagtacgggt actgggccga
     2641 ggtcgtgtac gcgtcgagga cccaggtgat ccgaccgttg acgacgaccg ggtacggatc
     2701 agagtcgaac tggaggaacg gggcgagctt ctggacccgg ctctggatgt ctcggaccat
     2761 gagaagcttg gagctgccgg tgacctggcc cgagatgagc gggttgatgt cgccaaagcg
     2821 gagcgcgaac gcggctttgc ggatgatgtt cgagagcttc acgccgtccc ggcccttgta
     2881 ccgggtggag gcgttggtgt tgttggcctg ggcgtagttg aactcgtcct gtttggcgtt
     2941 gacgatcacg tagccgctct ggttctcccc gaagtacagc tgggcgccct ggttggtgag
     3001 cgggatccca ttcgacgtcg gcgggatgtt cgacagcgtg aaggcggggt cgccgctcga
     3061 ggtggcctgg ttcgtcggcg acacgatcga cccgtagccg tgcgtgtaca cgacgtgctg
     3121 gttcacccac gactggctcg ggaggctgtt gctgttgagg tcgcgggccg caacgagtac
     3181 ctggctggtc tggccgttga tggtgtagcg gtcgatgtcg acgttgttga tgctgtagta
     3241 gggctggagc ccctggagcg tctggtaggt ggagcggatc accgccgggt cccagagccg
     3301 tgcgttctcg atcgtgcccc ggttcgtgtc cacgaccgac gggttgaggt tagcggtgta
     3361 gttgaacggg ttcaccgtga cgttgttcag cttgaacgca gcgcgggtgg cagtgatgtt
     3421 gcgcccgatg tagggccgtt cctgggcgag ctcgttgggc ttgacccgga agttctggat
     3481 cgcggccggg tagatcgtgc cgaccacgat ggacacgaag ccccagagtc cgacggcgat
     3541 gaccggcagg acccagccgc gacggaagat gttccagagg aacaggccgg cggcgacgac
     3601 ggagatgaag atgaggagat tgaacgccgg gagctgggct ttgacgtcgg tgtaggtagc
     3661 tccgtcgacg acgccgcgac cggagagcgt cagcccgaat cgggagaagt agtactgggc
     3721 ggtcttcacg agggccatca ccgcgacgac gaccgagagg tgcgccttga cctggggcgt
     3781 gacgcgctgc agcgggctct ggaaccggat cccgccgttc aggtagtgag cgaccgcggt
     3841 caccaacaac acgatcacga gcccggcgaa gagccagtcg aggatgaact ggatgaacgg
     3901 gagctggaag acgtagaagc cgacgtcctt gtggaactgc gggtctttga cgccaaagtc
     3961 gacccggtgc atgaacagga cccagttccg ccactgcgac gagaccccga tgccggcaat
     4021 cagcgcgaag aacgccgcga tgacgacgcg gatccgcccc tggtagcggc cggcgacctg
     4081 ctggtaccgg ttgacgagct cgtcttcggg gctggccgga cccatcggat gcaccttcgg
     4141 cgcgagccgg tcggcgatga ggagactggc cagcatgatc acgaagaaca ccgcggtgaa
     4201 caccaccgcg gggacgacct tggcgccgag gagcgcgctc cacgtcccgc cctggcccag
     4261 cgaatcgaac cacaggtagt ccgtgtagaa gccggccagc ccccgcaggc tgaacagcag
     4321 gatcacgagg accacgacga agccgatgat ccaggcgcgc agccggaacg atcgccgttc
     4381 catggtcggg acccgcattg ggcggcagcc tagacgtgcg gccccgggcc ccgaacgtcg
     4441 ccgggcacga ccaggcagcg gcatccggga tgcgccgggg ggtacggctg tccggtcggg
     4501 aacggctggc ccttcaccgt cggttcgagc
//
LOCUS       DATCCH010000027         2101 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694 k141_1710,
            whole genome shotgun sequence.
ACCESSION   DATCCH010000027 DATCCH010000000
VERSION     DATCCH010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1710"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="VIJ44_00575"
     CDS             <1..410
                     /locus_tag="VIJ44_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005871936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HEY5164420.1"
                     /translation="TGTLVPVNPIPTPTQPWVGGPYSGAVRAGDWIVLAGQVGVEPTS
                     GQVAAGDVVDQARQVFANLQAILADTGATWGDVAKVTIFLAVQDLGMMPALNALYTEH
                     VGEHRPARTTVGVAWLPLGALVEVEVWVYKPEA"
     gene            410..808
                     /gene="msrB"
                     /locus_tag="VIJ44_00580"
     CDS             410..808
                     /gene="msrB"
                     /locus_tag="VIJ44_00580"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEY5164421.1"
                     /translation="MSERAVERTDQEWQDELSPEEYDVLRRAGTERAFTGKYWDCHDD
                     GAYVCAGCGAPLFDSGTKFESGTGWPSFSEPAVTEGVELRQDRAHGMRRTEVVCRRCG
                     GHLGHVFDDGPGPNGQRYCINSTALNLEPR"
     gene            934..1800
                     /locus_tag="VIJ44_00585"
     CDS             934..1800
                     /locus_tag="VIJ44_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009576882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5164422.1"
                     /translation="MAYPDGIGVVDLMIGFPIRDRKGVYDRLLKPGLKDEESRQEFAF
                     PAEYMFKDVPGEADDEHDPIEVAFAEMDRFGIEVGLFGMSPDAIAARERDPKRVQLSL
                     EVNPNDVMGAIRKIRKAKAEHDLRAVTFFPSGCLPQVPVDDPKAYAVYAACVDLDLPV
                     VVNAGIAGPRFPSDCQHVMRFDQVCYDFPELRIVMRHGAEPWEELAVKLMLKWPGLSY
                     MTSAFAPKYYPKAIIDYANTRGADKVMYAGYYPMGLSLERIFSELPQVPFRDHVWPKF
                     LRENARSIFALD"
     gene            1803..>2101
                     /locus_tag="VIJ44_00590"
     CDS             1803..>2101
                     /locus_tag="VIJ44_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020555319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEY5164423.1"
                     /translation="MTDRYLLISADCHAGGSMEQYREYLDPEYRDEYDAWRGRYSNPF
                     RDLQSDGRTRNWDSERRVDELAADGVVAEVLFPNTVPPFFPTGAVIARPPRATNYA"
BASE COUNT          333 a          723 c          731 g          314 t
ORIGIN      
        1 cgactggaac cctggtgccc gtgaacccga tccccacacc gacccagccg tgggtcggtg
       61 ggccctactc gggcgcggtg cgcgccgggg actggatcgt cctcgccggg caggtcgggg
      121 tcgagccgac ctcgggtcag gtcgcggcgg gcgatgtcgt cgaccaggcc cgccaggtgt
      181 tcgccaacct ccaggcgatc ctcgccgata ccggcgccac ttggggggac gtggcgaagg
      241 tgacgatctt cctcgccgtg caggacctgg gaatgatgcc ggcgctgaac gcgctctaca
      301 ccgagcacgt cggtgagcac cgaccggcgc gcacgaccgt cggtgtcgcc tggctcccgc
      361 tgggggcgct ggtggaggtc gaggtgtggg tctacaagcc ggaggcgtga tgagcgaacg
      421 agccgtggaa cgaaccgacc aagaatggca ggacgagctg tcccccgagg agtacgacgt
      481 gctgcgccgg gccgggaccg agcgggcctt caccggcaag tactgggact gccacgacga
      541 cggcgcgtac gtctgcgcgg gctgcggggc gccgctgttc gactccggca ccaagttcga
      601 gtccgggacc ggatggccga gcttctcaga gccggcggtc accgaaggcg tcgaactgcg
      661 ccaggaccgg gcgcatggga tgcgtcgcac cgaggttgtc tgtcggcgct gcggtgggca
      721 cctcgggcat gtgttcgacg acgggcccgg ccccaacggc cagcggtact gcatcaactc
      781 cacggccctg aacctcgagc cccgctgacg gcccccgggc cggggacgct cccgcctccc
      841 ccgccggcgg ggcggtaggc ggcgggcgtg gttcgacccg gtgcgtcggg cccggctggg
      901 cacggccccg gcctggcgtg agacgatccc gggatggcgt accccgacgg aatcggcgtg
      961 gtggacctca tgatcgggtt cccgatccgg gaccgcaaag gcgtctacga ccgactcttg
     1021 aagcccggac tcaaggacga ggagagccgc caggagttcg cgttccccgc cgagtacatg
     1081 ttcaaagatg tccccggcga ggccgacgac gagcacgatc cgatcgaggt cgcgttcgcc
     1141 gagatggacc gcttcgggat cgaggtcggg ctgttcggga tgtcccccga tgccatcgcg
     1201 gcgagagaac gcgacccgaa gcgggtgcag ctgtccctgg aggtgaaccc gaacgacgtg
     1261 atgggcgcga tccgcaagat ccgcaaggcg aaggccgagc acgacctccg ggccgtcacg
     1321 ttcttcccgt ccgggtgcct cccccaggtt cctgtcgacg acccgaaggc gtacgcggtg
     1381 tacgcggcgt gcgttgacct ggacctgccg gtcgtcgtga acgccgggat cgccggaccg
     1441 cggttcccat ccgactgcca gcacgtcatg cgcttcgatc aggtctgtta cgacttcccc
     1501 gagctgcgga tcgtgatgcg ccacggcgcc gagccgtggg aggagctcgc ggtcaagctc
     1561 atgctcaaat ggcccggcct gtcgtacatg acgtcggcct tcgcaccgaa gtactacccg
     1621 aaggcgatca tcgactacgc gaacacccgc ggtgccgaca aggtcatgta cgccgggtat
     1681 tacccgatgg ggctgtccct cgagcggatc ttctccgagc tgccgcaagt gccgttccgc
     1741 gaccatgtgt ggccgaagtt cctccgcgag aacgcgcggt cgatcttcgc gctcgactag
     1801 ccgtgaccga ccgctatctg ctcatctcgg cggactgcca cgccggcgga tcgatggagc
     1861 agtaccgcga gtacctcgat cctgaatacc gcgacgagta cgacgcctgg cggggccggt
     1921 actcgaaccc gttccgggac ctccagagcg acggccgcac ccggaactgg gacagcgagc
     1981 ggcgagtcga cgagctcgcc gccgacggcg tcgtcgctga ggtcctgttc ccgaacaccg
     2041 tccccccgtt cttccccacc ggggcggtca tcgcccgacc accccgcgcc acgaactacg
     2101 c
//
LOCUS       DATCCH010000028         5403 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_126166, whole genome shotgun sequence.
ACCESSION   DATCCH010000028 DATCCH010000000
VERSION     DATCCH010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5403
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126166"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="VIJ44_00595"
     CDS             complement(<1..425)
                     /locus_tag="VIJ44_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEY5164424.1"
                     /translation="MSPRVLVTEELAARGLDAMRAAGLEVDQRLGLTPDELRDAVRDA
                     AALVIRSATRVDDALLAAAPELVVVGRAGIGLDNVDVAEATRRGVMVVNAPQSNVLSA
                     AEHTIALMLAQARNIPQAHADLVAGSWNRSRWEGVELNG"
     gene            complement(522..1712)
                     /locus_tag="VIJ44_00600"
     CDS             complement(522..1712)
                     /locus_tag="VIJ44_00600"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014178738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEY5164425.1"
                     /translation="MERLSRKISAITESATLAVDAKAKALRAAGEPVIGFGAGEPDFP
                     TPAHITEAAAAACLDPRNHRYTPTPGLPELREAIAAKTLRDSGYRCEASQVLVTNGGK
                     QAVYETFQALLNPGDEVLLPAPYWTTYPEAIALADGVSVPLATTEETGFRVSVDTLEA
                     ARTERTKALLFVSPSNPTGAVYPPAEVEAIGRWAVEHGVWVVTDEIYEHLTFGAHRFS
                     SMPALVPELADTCVVVNGVAKTYAMTGWRVGWMIGPRDVVASATNLQSHLTSNVTNVA
                     QRAAVAALGGDLDAVATMRATFERRGRTMHELLSAIPGVRCLEPQGAFYCFPSFAQVL
                     GREIAGRTPTSSLELCELALEEVKVAMVPGEAFGAPGYARMSFALGDEDLVEGVERLT
                     KLLR"
     gene            complement(1781..2956)
                     /locus_tag="VIJ44_00605"
     CDS             complement(1781..2956)
                     /locus_tag="VIJ44_00605"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY5164426.1"
                     /translation="MTPPSFDALGVSPDLVAAMHDQGIDEPFPIQVLTIPDALAGRDV
                     CGKAKTGSGKTLAFGLPLLERVGVAGSRRPTALVLVPTRELANQVFGVLGPLAATRGQ
                     RAVAVYGGVGFEPQINALRRGVEVVVGTPGRLIDLKERGDLVLSDVEVLVLDEADRML
                     DMGFLPQVQKLLYGVKSEPQTMLFSATLDGAIRRLVDRYMKDPVFHEVASRELTVDEM
                     DHHFLLVHQMDKPKVAAAIADGVDRTLIFTRTKRGADKLVQQLHREGVRAGAIHGDLR
                     QQARERALADFQAGKVPVLVATDVAARGLHIDAVDVVIHYDPAEDQTAYLHRSGRTAR
                     AGETGIAVTLMLYNQENVVRTVLRRLSLSNEIVEVFSNDPRLADLRSFDPDETTVPA"
     gene            complement(3109..3687)
                     /locus_tag="VIJ44_00610"
     CDS             complement(3109..3687)
                     /locus_tag="VIJ44_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HhH-GPD-type base excision DNA repair protein"
                     /protein_id="HEY5164427.1"
                     /translation="MSRPEIPVTGDPDADRLLVENAMALLIGMLLDQQVPMEWAFKGP
                     ATLQTRLGGLDASRIADLDPDDLDAAFRTKPAVHRFPSSMAKRTHALAQMIVDEYGGD
                     AAAIWRDVPSGAELFERLRALPGFGDEKAKIFLALLAKRLGVRPDGWEAAAAPFSDST
                     PRSVADIDSPETLARVRDWKRTQKARGRGKAD"
     gene            3709..4074
                     /locus_tag="VIJ44_00615"
     CDS             3709..4074
                     /locus_tag="VIJ44_00615"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5164428.1"
                     /translation="MRLDHVNLCVPTDGVPAEARWLVDILGYQTVEPGPEASRFGTLH
                     WFEAEDGTQVHLTIDAEHRPSGRAHTAIHVGDVLDDVIARVESSGQSTSAITFDGGRH
                     VFATDPAGNLWELIGPPAS"
     gene            4130..4807
                     /locus_tag="VIJ44_00620"
     CDS             4130..4807
                     /locus_tag="VIJ44_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011598958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5164429.1"
                     /translation="MRVLIVEDEVHLAAAITEGLEAEGFDVDAVHDGLDGLWRAREGS
                     YDAIVLDVLLPGMNGYRVCKTLREEGVWTPILILTAKDGEWDEAEALDTGADDFLSKP
                     FSFVVLLARLRALFRRGAPPRPAVLELGPLRLDPGTRRLTREGDEIRLTTREFAVLEY
                     LMRRDGTVASKAEILDHVWGIDFAGDPNVVEVYVGYLRRKIDQPYGTQLIRTVRGAGY
                     HLVRVGD"
     gene            4848..>5403
                     /locus_tag="VIJ44_00625"
     CDS             4848..>5403
                     /locus_tag="VIJ44_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164430.1"
                     /translation="MRLRVTLAAAGLVAVGLTGASFALVRSVHNNLEGEIRRTNQAQL
                     ALVAQQLAQGRDPSQLNLPLDPGAGGPPIIELRGPNGSREDLNYPRRIPTAQPPPLPT
                     GQAIRAQQTIATQRGQITLIAQRSLGEVNRTVDSVTASLFLGVPALVAAIAALAWYLA
                     GRALRPVESIRSEAAAITATTMHRR"
BASE COUNT          766 a         1820 c         1995 g          822 t
ORIGIN      
        1 ttgccgttca gctcgacacc ttcccaacgg ctgcggttcc agctcccggc gacgaggtcg
       61 gcgtgcgcct gcgggatgtt gcgggcctgg gcgagcatca gcgcgatcgt gtgctcggcc
      121 gccgacagca cgttcgactg cggcgcgttc acgaccatga cgccgcggcg tgtggcctct
      181 gcgacgtcga cgttgtcgag cccgatccca gcccggccga cgacgacgag ctcgggggcg
      241 gcggcgagga gggcgtcgtc gacgcgggtg gcgctgcgga tgacgagcgc cgccgcgtcg
      301 cgtaccgcgt cgcgcagctc gtcgggggtg agaccgagcc gttggtcgac ctcgaggccg
      361 gccgcgcgca tggcgtcgag accgcgggct gcgagctcct cggtgacgag aacgcgtggg
      421 ctcacgacgc gtgagggtag tggcgtgggc ggtcccgacg gcgattcgtt gtctgggtgt
      481 gctcgccgcg tcgaacacgt ccgcgggacc agagggccga ctcagcgcag cagcttggtg
      541 agccgttcga cgccttcgac gaggtcctcg tcgccgagcg cgaacgacat ccgggcgtac
      601 ccgggcgcgc cgaacgcctc gccgggaacc atcgccacct tgacctcctc gagcgccagc
      661 tcgcacagct cgaggctgct cgtcggggtg cggccggcga tctcgcggcc gaggacctgg
      721 gcgaaggagg ggaagcagta gaacgcgcct tgcggttcga ggcagcggac cccggggatg
      781 gccgacagca gctcgtgcat ggtgcggcct cgccgttcga acgtggctcg catcgtcgcg
      841 accgcgtcga gatcaccgcc cagggcggcg accgccgccc gttgcgccac gttcgtcacg
      901 ttcgaggtga ggtgggactg caggttcgtg gccgaagcga ccacgtctcg aggtccgatc
      961 atccatccga cccgccagcc ggtcatggcg taggtcttcg cgacgccgtt gacgaccacg
     1021 caggtgtcgg ccagctcggg gacgagcgcc ggcatcgacg agaagcgatg cgcgccgaag
     1081 gtcaggtgct cgtagatctc gtcggtgacg acccagacgc cgtgctcgac cgcccaccgc
     1141 ccgatcgcct cgacctcggc cggtgggtac acggccccgg tggggttgct cggggagacg
     1201 aagagcaacg ccttcgtccg ctcggttcgg gccgcttcga gggtgtcgac cgagacccga
     1261 aagccggtct cctcggtggt ggcgagcggg accgagacgc cgtcggcgag ggcgatggcc
     1321 tcggggtagg tcgtccagta cggggcgggg agcagcacct cgtcgccggg gttgaggagc
     1381 gcctggaacg tctcgtagac ggcctgcttg ccgccgttgg tgaccagcac ctgggacgcc
     1441 tcgcaccggt acccggagtc ccgcagggtc ttggccgcga tggcttcccg cagctcgggc
     1501 agccctgggg tcggcgtgta ccggtggttg cgcgggtcga ggcacgcggc cgcggcggct
     1561 tcggtgatgt gcgccggggt cgggaagtcc ggctcgccag caccgaaccc gatgaccggc
     1621 tccccagcgg cgcgcagggc cttcgccttg gcgtcgacgg cgagggtggc cgattcggtg
     1681 atcgcgctga tcttgcggga caggcgttcc acgggcccat cctcgcacga cgccggcgtc
     1741 cgacgggctg gtttcgccgc ggacgagggc gcgcggccgc tcaggcgggg acggtcgtct
     1801 cgtcggggtc gaaggaccgc aggtccgcga ggcgagggtc gttggagaac acctcgacga
     1861 tctcgttgga gagcgacagt cggcgcagca cggttcgcac cacgttctcc tggttgtaga
     1921 gcatcagcgt caccgcgatt ccggtctcgc cggcgcgggc ggtgcgcccg gagcggtgca
     1981 ggtacgcggt ctggtcctcg gcggggtcgt agtggatgac gacgtcgacc gcgtcgatgt
     2041 gcagccctcg agcggccacg tcggtggcga cgaggacggg aaccttgccg gcttggaagt
     2101 cggcgagcgc ccgttcgcgt gcttgctggc ggaggtcgcc gtggatcgcg ccggcccgga
     2161 cgccttcgcg gtgcagctgc tggacgagct tgtcggcgcc ccgcttggtg cgggtgaaga
     2221 tcaaggtgcg gtcgacaccg tcggcgatcg cggccgcgac cttcggcttg tccatctgat
     2281 ggacgagcag gaagtggtgg tccatctcgt cgacggtgag ttcccgcgag gcgacctcgt
     2341 ggaacacggg gtccttcatg taccggtcga cgaggcgtcg gatggcgccg tcgagcgtgg
     2401 cggagaacag catggtctgc ggctcggatt tgaccccgta gaggagcttc tgcacctggg
     2461 ggaggaaccc catgtcgagc atgcggtcgg cctcgtcgag caccagcacc tcgacgtcgc
     2521 tgagcacgag gtcgccgcgc tccttgagat cgatgagccg accgggggtt ccgacgacga
     2581 cctcgacgcc gcgtcgcaag gcgttgatct ggggctcgaa gccgacgccg ccgtagaccg
     2641 cgacggcccg ctggcctcgg gtcgcggcga gcgggccgag aaccccgaag acctggttgg
     2701 cgagctcacg ggtggggacc agcacgagcg cggtgggacg tcgggagccg gcgacgccga
     2761 cccgctcgag gagcgggagc ccaaaggcga gcgtcttgcc ggagccggtc ttggccttcc
     2821 cgcacacgtc tcgtccagcg agggcgtcgg ggatggtgag gacctggatc gggaacggtt
     2881 cgtcgatgcc ctggtcgtgc atggccgcga cgaggtcggg ggagacgcct agcgcgtcga
     2941 aactgggagg ggtcacgggg gaaatgctcg ctttcacgga tacggtcggg gtcgccgcgt
     3001 ggcgggggcg gcgaggtcgc cctctgtgac gtgagcccgg accgtccgga gaaaccgagg
     3061 atgacctgac gggcaccggc ggtgccgtcg ggtgccaatg ctaccccatc agtccgcttt
     3121 gcctcggcct cgggccttct gggtgcgttt ccagtcccgc acccgcgcca gcgtctcggg
     3181 tgaatcgatg tcggcgacgg aacggggcgt cgaatccgag aacggggccg cggcggcctc
     3241 ccatccgtcg gggcgtacgc cgaggcgctt ggcgagcaac gcgaggaaga tcttggcctt
     3301 ctcgtccccg aagccgggca gggcgcggag ccgttcgaag agctcggcgc cgctcggcac
     3361 gtcacgccag atcgccgcgg cgtcaccgcc gtactcgtcg acgatcatct gagccaacgc
     3421 gtgggtgcgt ttggccatcg agctcgggaa gcgatgcacc gcgggcttgg tgcggaacgc
     3481 ggcgtcgagg tcgtcggggt cgaggtcggc gattcgggag gcgtccaggc cgccgaggcg
     3541 ggtctgcaac gtggcggggc ctttgaacgc ccactccatc ggcacctgct gatcgagaag
     3601 catcccgatg agcaaggcca tggcgttctc cacgagcagc cgatcggcgt cgggatcccc
     3661 ggtgaccggg atctcgggac gactcatggc cacgtaggat cgcgcgtcgt gcgactcgac
     3721 cacgtcaacc tctgcgtccc gaccgacggg gtccccgcgg aggcccgctg gctcgtcgac
     3781 atcctcggct accagaccgt cgagccgggc cccgaggcgt cccgcttcgg gacgctgcac
     3841 tggttcgagg ccgaagacgg cacccaggtg cacctcacca tcgacgccga gcatcgcccg
     3901 tcgggtcggg cccacaccgc gattcacgtc ggcgacgtcc tcgacgacgt catcgcccgg
     3961 gtggagtcgt cgggccaatc gacatcggcc atcacgttcg acggcggccg gcacgtgttc
     4021 gccaccgacc cggccgggaa cctctgggag ctgatcggac cgcccgcttc ctgagcggtc
     4081 tctcagcgga ggccgcgctc gaccggtggc cacggtgcac aatccagagg tgcgagttct
     4141 catcgtcgaa gacgaggtgc acctcgccgc ggccatcacc gagggcctcg aagccgaagg
     4201 gttcgatgtc gacgccgtcc atgacggcct cgacgggctg tggcgggcgc gggaaggctc
     4261 gtacgacgcg atcgtgctcg acgtgctcct ccccgggatg aacggctacc gcgtgtgcaa
     4321 gacgctgcgg gaggaaggcg tgtggacgcc gatcctgatc ttgaccgcca aggatggtga
     4381 atgggacgag gccgaggcgc tcgacaccgg cgccgacgac ttcctctcga agccgttctc
     4441 gttcgtcgtg ctgctcgccc ggctgcgggc gttgttccgc cgcggcgcgc caccgcggcc
     4501 ggcggtgctc gagctcggcc cgctgcgcct cgatcccggg acccggcgcc tcacccgtga
     4561 aggcgacgag atccgcctca ccacccgcga gttcgcggtg ctcgagtatc tgatgcgccg
     4621 cgacggcacg gtggcgtcga aggctgagat cctcgaccat gtgtggggaa tcgacttcgc
     4681 cggcgacccg aacgtggtgg aggtctacgt cggctatctg cgccgcaaga tcgaccagcc
     4741 gtacgggacg cagctgatcc gcacggtgcg gggcgcgggg tatcacctcg tgcgtgtcgg
     4801 ggactgacgt gtcgatccgt tcgttcgcga gtcgtcggct cggatcggtg cgtctacgcg
     4861 tcaccctcgc cgccgccggt ctcgtcgcgg tcggtctcac cggagcgtcg ttcgcgctgg
     4921 tgcgctcggt ccacaacaac ctcgaagggg agatccgccg gacgaaccag gctcagctgg
     4981 cgctggtcgc ccagcagctg gcgcagggcc gcgacccgag ccagctgaac ctgcctctcg
     5041 accccggtgc gggcgggccc cccatcatcg agctgcgcgg acccaacggg agccgcgagg
     5101 acctcaacta cccgcgtcgc atcccgaccg cgcaaccccc gccgctcccg acggggcagg
     5161 cgatccgagc ccagcagacc atcgcgaccc agcggggtca gatcacgttg atcgcgcaac
     5221 gctcactcgg cgaggtgaac cggaccgtcg acagcgtcac cgcttcgctc ttcctcggtg
     5281 tcccggcgct cgtcgcggcg atcgccgcgt tggcctggta cctggccggc cgggcgctcc
     5341 gcccggtcga atcgatccgg tccgaggcgg ccgcgatcac cgccaccacg atgcaccgtc
     5401 gcg
//
LOCUS       DATCCH010000029         3091 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694 k141_1862,
            whole genome shotgun sequence.
ACCESSION   DATCCH010000029 DATCCH010000000
VERSION     DATCCH010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1862"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VIJ44_00630"
     CDS             <1..333
                     /locus_tag="VIJ44_00630"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY5164431.1"
                     /translation="WRESEWHENALSAAGAVGIGQLLPPTSTFVAQDLLHQPGLDPRR
                     PVDNIRLTARYLRELINTFHGEERLGVGAYLQGSTSVASQGLTPQTTDYVNQIESLRA
                     EFARARRG"
     gene            complement(352..537)
                     /locus_tag="VIJ44_00635"
     CDS             complement(352..537)
                     /locus_tag="VIJ44_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164432.1"
                     /translation="MAVEHIKERLHAALHRGGTKASEEQLEAVTEVVLLVVAEVTAEI
                     AVLIADLEERVVALETA"
     gene            complement(642..1055)
                     /locus_tag="VIJ44_00640"
     CDS             complement(642..1055)
                     /locus_tag="VIJ44_00640"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY5164433.1"
                     /translation="MPELSTEDKRAALAKVEFFAGCSERQLADVAHLAVERDLDAGAQ
                     LCHEGEFEQHAFVILDGEATASIGSSAVGSVGPGEVVGELAMLGDGHRKATLVARTPL
                     RVLVLDADDIDSVLAADPHAQENLGPRATRAGDDG"
     gene            1267..>3091
                     /locus_tag="VIJ44_00645"
     CDS             1267..>3091
                     /locus_tag="VIJ44_00645"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY5164434.1"
                     /translation="MSKNPPGRRPGDPASAATTPSGSHVEPERRWSGFLEWASSPRGA
                     WLVDFTALAILSVALPLVIIWHFGALGIPRNDDWAFTRILFSWTRTGHLQLLDWNTQT
                     LVGQLAITVPVVKVFGTRIGALQAWVAVLGGAGCCCLYVVLRRLLPRVSALAALGCLL
                     VGPIYGSLAGSFMSDVPAFAAACGCLWAGVVALERRGWSFAGWLAGSAVLGFLALSIR
                     QVAVTAPIAVALALLVVVRRGRRPAASERTAAIERWASVGIALVFTVGTAALLVWRNG
                     LPFSNSIRLSLHPAFGPHNSFLYEILFTLALLSLPAVLRLSPVAVLRRAVAVSRAATV
                     VVVAGWAIVVGGALYLSRTSQVDLVLGNYVTRQGTVGVLAGHRPDLLPSGAWTAVVAA
                     SVYAALMLALVIVTRLPAARRSVDAFRGGFIVDSANVAGLVLGGFGVLYVMALELALG
                     FTQVVPSGGVAPAFSQTPYDRYLIPLVPIVVVLAWSDPSLFWPAPRVRRVVTAAGFGL
                     FAVLGLAYVVESASYDGARWAAASQLARSGVPAAAIDGGAEWVGFHAGTAAIRHSGPS
                     PTSWASMFRDVRTCYLISMAPHYTRHGVTGPIATFRYRGWHP"
BASE COUNT          375 a         1087 c         1074 g          555 t
ORIGIN      
        1 tggcgcgaat cggagtggca cgagaacgcg ctgtccgccg ccggcgccgt cggcatcgga
       61 cagctgctgc cgccgacgtc gaccttcgtg gcgcaggacc tgctccacca gcccgggctc
      121 gatccgaggc gtcccgtcga caacatccgg ctgacggccc gctacctccg cgagctcatc
      181 aacacgttcc acggtgagga acggctcggg gtcggcgcgt atctccaggg ttcgacgtcg
      241 gtggcgagcc agggtctgac gccccagacc accgactacg tgaaccagat cgagtccttg
      301 cgcgccgagt tcgcacgggc gcgtcgcggt tgacccgggt tggcgttgag ctcaggccgt
      361 ctccagcgcc acgactcgct cttctaggtc agcgatcagc acggcgatct ccgccgtcac
      421 ctccgcgacg accaggagca cgacttcggt gacggcctcg agctgctcct cggaggcctt
      481 cgtgccgccc cggtggaggg cagcgtgcaa ccgctctttg atgtgttcga cagccatcgg
      541 gcgaatgtaa ccggtcttac ctggtcgatc gtgatcgaga cgaggccgac gagagcgcgg
      601 ggaggtgagg acccttggaa acgacggtga cgagcgggcc gctacccgtc gtcgccggcc
      661 ctcgtggcac gaggaccgag gttctcctgc gcgtggggat cggccgcgag caccgaatcg
      721 atgtcgtcgg cgtcgagcac gagcacccgc aatggcgtgc gcgcgaccag cgtcgccttt
      781 cgatgcccgt cgccgagcat cgccaactcg ccgacaactt cgccgggtcc aaccgatccc
      841 acggccgagc tcccgatact ggctgtcgcc tcaccgtcga ggatcacgaa ggcatgctgt
      901 tcgaactcac cctcgtggca cagctgggct ccggcatcga ggtcccgctc gacggccagg
      961 tgcgcgacat cggcgagctg gcgctcgctg cagccggcga agaactcgac cttcgcgagc
     1021 gcggcgcgct tgtcctcggt gctcaactcc ggcatcggct acccctcgcg tcgtgccgcg
     1081 gacctgaacc tggcttctag tcggtcccgc gtaccctgtc caggcatcgg gcgtcccagc
     1141 gcgtggcacg gcgccaggga agctcgctga tcgtggggtt gcggttcgac cgcctcaccc
     1201 tgacgcatcc acctggggtg gcaccgcagc ccatttggcg gtcgctccgc cgacgagagg
     1261 gccgaggtgt cgaagaaccc gccgggccgg cgccctggag accccgcgtc ggccgcgacg
     1321 accccgtccg gatcgcacgt cgaaccggag cgccgctggt ctgggttcct cgagtgggct
     1381 tcgagtcctc gcggggcgtg gctggtcgac ttcacggcgt tggccatcct gagcgtggcg
     1441 ctgccgctgg tgatcatctg gcacttcggt gctctcggca ttccccgcaa tgacgactgg
     1501 gcgttcacgc gcatcctctt ttcgtggacc cgcaccggtc atctccaact gctcgactgg
     1561 aacacccaga cgctggtcgg ccagctcgcg atcacggtcc cggtcgtcaa ggtcttcggg
     1621 acccggatcg gtgcgctcca agcctgggtc gcggtcctcg gcggggccgg ttgctgctgc
     1681 ttgtacgtgg tgctccggag gctgcttccg cgggtgagtg ccctggcggc gctcggctgc
     1741 ctcctcgtgg ggccgatcta cgggtcgctc gccgggagct tcatgagcga cgtgccggcg
     1801 ttcgcggctg cctgcggttg cctgtgggcc ggcgtcgtag cgctcgaacg ccgcggctgg
     1861 tcattcgccg gctggctcgc agggagcgcg gtgctcggct tcttggcact gtcgatccgc
     1921 caggtcgcgg tgactgcccc catcgcggtg gccctggccc tgctcgtggt ggtgcgacgg
     1981 ggccgtcggc ccgccgcgtc ggaacgcacc gcggcgatcg aacgctgggc cagcgtcggc
     2041 atcgcgttgg tgttcacggt cgggaccgcc gcgctgttgg tctggcggaa cgggctgccg
     2101 ttcagcaact cgatccggtt gagcctgcat ccggcgttcg gcccccacaa ttcgtttctc
     2161 tacgagattc tgttcacgtt ggcgctgctc agccttccgg cggtgctgcg cctgtctccg
     2221 gtcgcggtgc tgcgccgggc ggtcgcggtg agccgggccg cgacggtcgt ggtcgtggcg
     2281 ggctgggcga tcgtcgtggg tggcgcgctg tacctgtccc ggacctccca ggtcgacctc
     2341 gtgctcggga actacgtgac acgccagggg accgtcggcg tcctcgccgg gcaccgtccc
     2401 gacctgctgc ccagcggcgc gtggaccgcg gtggtcgcgg cgagcgtgta cgccgcgctc
     2461 atgctcgctc tcgtgatcgt gacccggctc cccgcggcgc ggcgatcggt ggacgcgttc
     2521 cggggtggct tcatcgtgga ttcggcgaac gtcgcgggcc tcgtgctcgg tggtttcggg
     2581 gtgctctacg tcatggcact ggagctggcc ctcggcttca cccaggtggt gccgagcggt
     2641 ggggtcgcgc ccgcgttcag ccagacgccg tacgaccggt atctgattcc cctcgtcccc
     2701 atcgtcgtcg tgctggcctg gagcgacccg tcgctgttct ggcccgcgcc ccgggtccga
     2761 cgcgttgtca ccgccgccgg tttcgggctg ttcgccgtcc tcggactcgc gtacgtcgtc
     2821 gagtcggcgt cgtacgacgg ggcccgctgg gctgccgcgt cccaactcgc caggtccggt
     2881 gttccagcgg cggcgatcga cggtggcgcc gaatgggtcg ggttccatgc cgggaccgcc
     2941 gcgatccgac acagcgggcc gagccccacg tcgtgggcgt caatgtttcg cgacgtgcgg
     3001 acctgttacc tcatctccat ggcacctcac tacacccgcc acggcgtgac cgggccgatc
     3061 gccacgttcc gctatcgggg atggcatccc c
//
LOCUS       DATCCH010000030         2539 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_294037, whole genome shotgun sequence.
ACCESSION   DATCCH010000030 DATCCH010000000
VERSION     DATCCH010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294037"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..827
                     /locus_tag="VIJ44_00650"
     CDS             <1..827
                     /locus_tag="VIJ44_00650"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY5164435.1"
                     /translation="TPFLDELPPGMLGVGERVMVMPKYRGTGLVDELLECRRERAANH
                     DVRIVFSACEPHLLSLYLSQGRKTYADKNINGVEAGYLIPLVSFPQGPDVLEALDKEL
                     GTIPDCVDRVLSNRGGAVLSPLMTPSGEYPDQIRQTLHEIEDQGVSAFHGFTDDEIER
                     CLTRSNVIECAAGDRVLKKGGVARNIFVVLGGTLEVRDEERVVAVLRTGDVFGEMAFL
                     LERPRVLDTWAATDSVRVLSLSESTLRKMIAEDPVVAAKLLMNLAKMLCVRLIKST"
     gene            complement(920..2029)
                     /locus_tag="VIJ44_00655"
     CDS             complement(920..2029)
                     /locus_tag="VIJ44_00655"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY5164436.1"
                     /translation="MSPDADHDTGRDLLHGVRVLDLSIWRPGPYATQLLADLGADVIK
                     LEPPGGDPMRVFPQLFATLNANKRSIVLDLHEPDQRAQAHALVAEADALVEGFRPGVA
                     ARLGMGEPEVRAINPSIVYCSVSGYGQDGPLAKTPGHDLNYQAYGGMLTPTGGTPVEC
                     TVPIADLSGGVFAAMATCAALLGRTRTGSGERIDVAMAEVLATWTGPFTGTAVAGQRE
                     PMGKLPSYGSFATADGQWITLGIIDEQHFWVRLCDVLDLADIRDLDLVARVTRHSEIA
                     GRLSTAIAGRSRDKLVAALGDDVPVAPALTRAEMLVHPQFVHRGTVGLRTSDGTPTMG
                     YPVRFTRQPARAPGRPPSLAEHQDEGFRPRPARAR"
     gene            complement(2070..>2539)
                     /locus_tag="VIJ44_00660"
     CDS             complement(2070..>2539)
                     /locus_tag="VIJ44_00660"
                     /inference="COORDINATES: protein motif:HMM:NF025668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin type III-like domain-contianing
                     protein"
                     /protein_id="HEY5164437.1"
                     /translation="DRRAHHVEYGLFHGQQHHDHVGRPPAYPLGWGLSYTDFRYGPPE
                     LAVHDDHLDISVEVTNTGRRSGAEVVQCYAAAPDSTVERPHRWLVGFQRVPLDPGASA
                     QVSIRVPLECLAYWDESRDQFVVEATRYDLVVAPHAAAPGQAVSAEIGEHVIT"
BASE COUNT          398 a          815 c          917 g          409 t
ORIGIN      
        1 tcacgccgtt cctcgatgag ctgccacccg gcatgcttgg cgtgggggag cgggtcatgg
       61 tcatgccgaa ataccgcggt accggcctgg tcgacgagct gctcgaatgc cggcgcgagc
      121 gcgccgcgaa ccatgacgtc cggatcgtgt tcagcgcgtg cgagccgcat ctcttgtcgc
      181 tctatctcag ccagggccgc aagacgtacg cggacaagaa catcaacggc gtcgaggcgg
      241 gctacctcat cccgctcgtc tcgttccccc aaggccccga cgtcctcgag gccctcgaca
      301 aggagctcgg gaccattccc gactgcgtcg accgtgtgct gtccaaccga ggcggcgcag
      361 tgctgagccc actgatgaca ccttcagggg aatatccgga tcagatccgc cagaccctcc
      421 acgagatcga agaccaaggc gtgtcggcgt tccacgggtt caccgacgac gagattgagc
      481 gctgcctcac ccggagcaac gtcatcgaat gcgccgccgg tgaccgcgtc ctcaaaaagg
      541 gtggcgtagc ccggaacatc ttcgtcgtgc tgggtgggac gctcgaggtt cgcgatgaag
      601 aacgagttgt cgccgtcctg cgaactggtg acgtcttcgg tgagatggcg ttcctgctcg
      661 agcgcccacg cgtccttgat acctgggccg ccaccgactc cgttcgagtc ctcagcctca
      721 gtgagagcac gctccggaaa atgatcgccg aagacccggt cgtcgcggcc aagctcctga
      781 tgaacctcgc caagatgctg tgcgtgcgac tcatcaagtc cacgtgattg ctgccccgca
      841 gcacgcgccg tggtggtggg tcggccacca gcatccctga ttgctcgcgg tccgtggagc
      901 ccgacgacga acgcggcgct tacctagcac gcgccggtcg cggccggaat ccttcgtctt
      961 gatgctccgc gagggacggt gggcgtccgg gtgcccgggc cggctggcgg gtgaagcgca
     1021 cgggatagcc catcgtcggc gtcccgtcgc tggtcctgag gccgacggtg ccccggtgga
     1081 cgaactgggg gtgcacgagc atctcggctc gggtcagtgc gggtgcaacc gggacgtcgt
     1141 cgccgagcgc ggcaacgagc ttgtcgcgtg accgacccgc gatcgccgtc gagagccgcc
     1201 cggcgatctc gctgtgacga gtcactcgcg cgaccaggtc gaggtcccgg atgtccgcga
     1261 gatcgagcac gtcacagagc cgcacccaga agtgctgctc gtcgatgatc cccagggtga
     1321 tccactgccc atcggcggtt gcgaacgacc cgtacgaagg gagcttgccc atcggctcgc
     1381 gctggccggc gacggcggtc ccggtgaagg ggccggtcca cgtggcgagg acctcggcca
     1441 tggcgacgtc gatgcgttca ccgctgccgg ttcgagtgcg gccgaggagc gcggcgcacg
     1501 tggccatcgc cgcgaagacc ccgccgctga gatccgcgat tggcaccgtg cactcgacgg
     1561 gtgttccacc ggtcggcgtg agcatcccgc cgtaggcctg atagttgagg tcgtgccccg
     1621 gtgtctttgc gagaggaccg tcttggccgt agcccgagac ggagcagtac acgatcgagg
     1681 ggttgatggc ccgcacttcc ggttcgccca tcccgaggcg ggcggcgaca ccgggccgga
     1741 atccttcgac gagagcatcg gcttcggcga cgagcgcgtg ggcttgggcg cgctggtcgg
     1801 gttcgtggag gtcgaggacg atgctgcgct tgttggcgtt gagggtggcg aacagctgcg
     1861 ggaagacgcg catggggtcg ccgcccggag gctcgagctt gatgacgtcg gcgccgaggt
     1921 cggcgaggag ctgggtggcg taggggccgg ggcgccagat gctcaggtcg aggacgcgaa
     1981 cgccatggag gaggtcgcgg ccggtgtcgt ggtccgcatc tggggacacc gcgggatctt
     2041 acgggccgag ccctacccgc tccgcgatgt caggtgatga cgtgctcgcc gatctcggcc
     2101 gacacggcct gtcccggggc agcggcgtga ggcgcgacga cgaggtcgta ccgggtggct
     2161 tcgaccacga actggtctcg ggattcgtcc cagtacgcga ggcactcgag cggaacgcgg
     2221 atcgagacct gggcgctggc gcccgggtcg agggggacgc gctggaagcc gaccagccat
     2281 cggtgcggtc gctcgacggt cgagtcggga gcggcggcat agcactgcac aacctcggcg
     2341 cccgaccggc ggccggtgtt ggtcacctcc acgctgatgt cgaggtggtc gtcgtggacc
     2401 gcgagctcgg gcggcccgta ccggaagtcg gtgtacgaga gaccccatcc gaggggatac
     2461 gccggcgggc gtccgacgtg gtcgtggtgc tgctggccgt ggaacaggcc gtactcgacg
     2521 tgatgggccc gacggtcaa
//
LOCUS       DATCCH010000031         2914 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_169636, whole genome shotgun sequence.
ACCESSION   DATCCH010000031 DATCCH010000000
VERSION     DATCCH010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169636"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VIJ44_00665"
     CDS             complement(<1..215)
                     /locus_tag="VIJ44_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012894898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164438.1"
                     /translation="MVAFHLSASDEGRHLAGTDALWGESWYHDFAAADGSYGGYARLG
                     LYPNLGVAWYWVHLVRRGQPLVLIRDHT"
     gene            complement(243..1007)
                     /locus_tag="VIJ44_00670"
     CDS             complement(243..1007)
                     /locus_tag="VIJ44_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017571637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5164439.1"
                     /translation="MGVIAVTGSAGGIGGAIRRQIEAEGHDVIGVDVRDAEVVADLSS
                     AAGRSEALDGIREAAGPALDGLVVAAGIGGSTSAPPSMVARINYFGAVALLDGLREPL
                     ARGALHAAVAISSNSATAVPAQDLTLVEHCLSGREDEAADHADTLDGELVYAHAKLAL
                     ARRVRRLAVEWAPDVRVNAVAPGPVLTPLTQAALEHPVTGDLIRSYPIPLDRWGEPEE
                     IAAGVWFLLSPAAAWITGVLLFIDGGTDALLRPDGI"
     gene            1099..2817
                     /locus_tag="VIJ44_00675"
     CDS             1099..2817
                     /locus_tag="VIJ44_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007492909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5164440.1"
                     /translation="MLDMVVRGGEVIDGTGAPRRRADVGVRAGRVVTVGDVDEPAATT
                     VDAEGKVVTPGFVDIHTHYDAQVLWDPATTPSPFHGVTSVVGGNCGFTIAPVEPSETE
                     YLTRMLARVEGMPLESLIAGVPFDWRTFGEYLDRLDGNLAVNAGFLVGHSAIRRVVMG
                     DDAVGHTATDDQIDAMTRLLGESIAAGGLGFSSSQAKTHNDGNGDPVPSRHATPDELV
                     ALAREAGRHEGTTLEFIPSVGGFEEAHLALMAAMSREADRPLNWNVLVPNAARSEVAW
                     KMLQASDYAAEHGGKVLALTVPDVMKTHLTFKAGFVFDALPGWNKTMALEPAEKMHAL
                     ADPSERQRLRDAAASPEAGMLRGVANWANMTIVETFAPENAPLVGRRIGDITEERGGD
                     PFDVLLDIVLADELRTVLLPPLPGDDQASWELRRDIWRDPRAVVGASDAGAHLDMLST
                     FSYSTAMLRSCRDRGLMALEEAVHLLTDRQARLYGLRERGRVAEGWYADLVVLDEERV
                     APAPVVWRNDLPAGAGRLYAGAEGIDHVVVNGVEIVTDGELTGAVPGTLLRSGRDTET
                     VTASRA"
BASE COUNT          450 a         1047 c         1015 g          402 t
ORIGIN      
        1 gtgtgatccc ggatgagcac gagcggctga cctcgacgga ccaggtgcac ccagtaccag
       61 gcgacaccga ggttcgggta caggccgagg cgggcgtagc cgccgtagga gccgtccgcg
      121 gcggcgaagt cgtggtacca cgattcgccc cagagggcgt cggtgccggc gaggtgtcgt
      181 ccctcgtcgc tggcactcag atgaaacgcg accacggtgc acctcgctgg gttcgcatcg
      241 gcctagatcc cgtcaggacg gagcagcgcg tccgtgccgc cgtcgatgaa gagcaggact
      301 ccggtgatcc acgccgccgc cggggacagg agaaaccaga cgcccgcggc gatctcctcg
      361 ggttcgcccc agcgatcaag cgggatgggg tacgagcgga tgaggtcacc ggtgaccgga
      421 tgctcgagcg cggcctgggt cagcggggtc agcaccggac cgggcgcgac agcgttcacg
      481 cgcacgtcgg gcgcccactc caccgcgagg cgtcggacac gccgggccag agcgagcttg
      541 gcgtgcgcgt acaccagctc cccgtccagg gtgtcggcat ggtcggccgc ttcgtcctcc
      601 cggccggaga ggcagtgctc gacgagggtg aggtcctggg ccgggaccgc ggtagcagag
      661 ttcgacgaga tcgccaccgc ggcgtgcagc gcccctcggg cgagaggctc ccggagcccg
      721 tcgaggaggg cgaccgcgcc gaagtagttg atccgggcaa ccatcgaagg gggcgcggac
      781 gtgctgccac cgatcccggc ggccacaacg agcccgtcga gcgcgggccc cgcggcctcc
      841 cggatcccgt ccagggcctc gctccgcccc gcggccgacg acaggtcggc gaccacttcg
      901 gcgtcgcgaa cgtcgacgcc gatcacgtcg tgaccctcgg cttcgatctg tcgacggatc
      961 gccccgccga tcccaccggc ggacccggtc accgcgatga cgcccatctc cgcattcgct
     1021 ccttgctggt gacacccggg ccgggaacga gtggcgacgc tagcctcggt tgcgatcgtc
     1081 caggcgaaag ggcccgtgat gctcgacatg gtggtacgtg gaggcgaggt catcgacggt
     1141 accggtgcgc cccgacgtcg cgctgacgtt ggggtgcggg ccgggcgggt cgtcaccgtc
     1201 ggcgacgtcg acgagcccgc cgcgaccacc gtcgacgccg agggcaaggt cgtgaccccc
     1261 ggcttcgtcg acatccacac ccactacgac gcccaggtgc tctgggaccc cgccaccacg
     1321 ccgtcgccgt tccacggcgt gacgagcgtg gttggcggca attgcgggtt cacgatcgca
     1381 cccgtcgagc ccagcgagac cgagtacctg acccggatgc tcgcccgcgt cgagggaatg
     1441 ccactcgagt cgctcatcgc cggtgtcccg ttcgactggc gcaccttcgg cgagtacctc
     1501 gaccgcctcg acggcaacct ggccgtgaac gccggcttcc tcgtcggtca cagcgcgatc
     1561 cgacgggtcg tgatgggcga cgacgccgtc ggccacaccg ccaccgacga ccagatcgac
     1621 gccatgacca ggctgctcgg cgaatccatc gccgccgggg gcctcgggtt ctcctcgtcg
     1681 caagccaaga cccacaacga cggcaacggc gacccggtcc cctcccggca cgccacgccc
     1741 gacgagctgg tggccttggc ccgcgaagcg gggcgccacg aaggcaccac gctcgaattc
     1801 atcccctccg tcggcggatt cgaagaagcc cacctggccc tgatggccgc catgtcccgg
     1861 gaagcggacc ggcccttgaa ctggaacgtc ctggttccga acgcagcccg gtcggaggtg
     1921 gcctggaaga tgcttcaggc gtcggactac gccgccgagc acggcggcaa ggtcctcgcc
     1981 ctgaccgtcc ccgacgtgat gaagacccat ctcacgttca aggccgggtt cgtgttcgac
     2041 gcccttcccg gttggaacaa gaccatggcg ctcgaaccgg ccgagaagat gcacgccctc
     2101 gccgacccgt ccgaacggca gcggctccgc gacgccgcgg cgtcacccga ggccggcatg
     2161 ctccgaggtg tcgcgaactg ggcgaatatg acgatcgtcg agacgttcgc gcccgagaac
     2221 gcccctctgg tcggacggcg catcggcgat atcaccgagg agcggggtgg ggatccgttc
     2281 gacgtgctgc tcgacatcgt gctcgccgac gagctccgca ccgtgctgct ccctccgctc
     2341 cccggggacg accaggcgtc gtgggagctg cgccgcgaca tctggcggga tccccgcgcc
     2401 gtcgtgggcg catcggacgc cggcgcgcac ctcgacatgc tctccacctt ctcgtacagc
     2461 accgcgatgc tgcgctcgtg tcgagaccgg ggtctgatgg ccctcgaaga ggcggtgcac
     2521 ctcctcaccg accgccaggc ccggctgtac gggctgcgcg agcgcggtcg cgtcgccgag
     2581 ggctggtacg ccgacctcgt cgtcctcgac gaagagcggg tcgccccggc accggtggtg
     2641 tggcgcaacg acctcccggc cggcgcgggc cggctctacg ccggcgccga gggcatcgac
     2701 cacgtggtcg tgaacggggt cgagatcgtg accgatggcg agctgaccgg cgcggttccc
     2761 ggcacgctgc tccggtccgg gcgcgacacc gagaccgtta ccgcatcaag agcctgagcc
     2821 gcctgaggtc cggtacctca ccaggcgggc ggctcgaacc cggttcgagg tgtcgttagg
     2881 cccagacgag cggaaggcgc taggcttggc ggat
//
LOCUS       DATCCH010000032         2221 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_460494, whole genome shotgun sequence.
ACCESSION   DATCCH010000032 DATCCH010000000
VERSION     DATCCH010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460494"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VIJ44_00680"
     CDS             complement(<1..390)
                     /locus_tag="VIJ44_00680"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEY5164441.1"
                     /translation="MTLTGPPLVAERGVPSDDARAATRGSLALTVLQTAGRLLAVGFV
                     LVATRVLAPGSFGRYSTASAFVVIGGLLADFGTTTVIVRRVSRDPDGSDTLLTRTVLA
                     CLGLGVASALAVAAVAVVAGYPAATRTD"
     gene            complement(387..1694)
                     /locus_tag="VIJ44_00685"
     CDS             complement(387..1694)
                     /locus_tag="VIJ44_00685"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEY5164442.1"
                     /translation="MARLAVNAGTVVCVLALGKAHAVAHGYSMTGSSRFAWSLGYALA
                     LVVAAYGFGLPEVPRTRRRAVFAAVGAAAVAAAAISVVELLVGDALLPRAVVFGSAVV
                     LVPWYVLWSRVAGDARSRAEERDRVVLVGDIDDVDVLRADLDRAPERNAVVVAAVTPE
                     QVALDDPPRLPLVELADAGRATVLVLSRRAQSDDDVVLQAARLHETGMRVRTLSLFYE
                     QWLGKLPVNELERVSLLFDVGELHAPRYARVKRLVDLMLAGIGAVALVVTTPVVTVVN
                     LAGNRGPLLYRQPRVGRDGRVFPILKFRTMSTVAPAGDPTVERDPRVTRFGRVLRSTH
                     LDELPQVLNILRGELSIVGPRPEQPHVVHELAGKLPFYRLRHLVRPGLTGWAQVKYQY
                     AGTEAEALEKLQYEFFYLRRQSLGLDLRIVGRTIRSVVGRDGR"
     gene            complement(1762..>2221)
                     /locus_tag="VIJ44_00690"
     CDS             complement(1762..>2221)
                     /locus_tag="VIJ44_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164443.1"
                     /translation="GRYLLLSGLLLAVVAAVALESAPWRSAAAVVVVAGTITGAGLAW
                     LSVRSHAFARAVSAAAAEPGPVVTTDLPHFWREGGGFYTPGRRWLTAETTAQLPAAVD
                     VVRRLDAPSFTLVSRTPGEAPQRLGPYRRGSRQALPVVSGIDVAVSRYHR"
BASE COUNT          343 a          821 c          815 g          242 t
ORIGIN      
        1 gtcggtgcgg gtcgccgcgg ggtagccagc gacgacggcc accgccgcga ccgcgagtgc
       61 cgatgcgact ccgagcccga ggcaggccag gacggtgcgg gtcagcagcg tgtcggaccc
      121 gtccgggtcg cggctcaccc gccgcacgat gacggtggtg gtcccgaagt cggcgagcaa
      181 cccgccgatc acgacgaacg ccgacgcggt cgagtagcgg ccgaacgacc caggggcgag
      241 gacccgggtg gcgacgagca cgaacccgac cgcgaggagc cgccccgcgg tctggaggac
      301 ggtcaacgcc agcgacccgc gagtcgcggc ccgagcgtcg tcggacggta cgccgcgctc
      361 ggcgaccagc ggcggcccgg tcagcgtcat cgtccgtcgc gcccgaccac cgagcggatg
      421 gtgcgaccaa cgatccgcag gtcgagtccg aggctctggc gccgcaggta gaagaactcg
      481 tactggagct tctcgagcgc ctcggcttcg gttcccgcgt actggtactt gacctgcgcc
      541 cagcccgtga gcccgggccg gacgagatgc cgcaaccggt agaacgggag cttgcccgcc
      601 agctcgtgaa cgacgtgggg ctgctcgggc cggggcccca cgatcgacag ctcgccgcgc
      661 aagatgttga ggacctgggg cagctcgtcg agatgcgtcg agcgcaggac acgcccgaac
      721 cgggtcaccc gcgggtcgcg ttccacggtg gggtcaccgg ccggcgcgac ggtgctcatg
      781 gtccggaact tcaggatcgg gaacacccgg ccgtcccgcc cgacgcgcgg ttggcggtac
      841 aggagcggac cccggttgcc ggcgaggttc acgaccgtca ccaccggcgt ggtcacgacc
      901 agcgcgaccg cgccgatccc cgccagcatc aggtcgacga gccgcttcac ccgcgcgtac
      961 cggggggcgt ggagctcccc gacgtcgaag agcaacgaga ctcgctccag ctcgttgacc
     1021 ggcagcttgc cgagccactg ctcgtagaac agcgagaggg tccgcacccg catcccggtc
     1081 tcgtgcagcc gcgccgcttg cagcacgacg tcgtcgtcgc tctgcgcccg acggctgagc
     1141 accagcacgg tggcgcgtcc cgcgtcggcc agctcgacca gcgggagtcg gggcgggtcg
     1201 tcgagcgcga cctgctccgg cgtcaccgcg gccacgacga ccgcgttgcg ttccggggcc
     1261 cggtcgaggt ccgcgcggag gacgtcgacg tcgtcgatgt cgccgacgag cacgacccgg
     1321 tcccgctcct cggctcgcga ccttgcatcg ccggcgacac gagaccacag cacataccaa
     1381 gggacgagca ccaccgcgga cccgaacacc acggcccggg gcaggagcgc gtcgccgacc
     1441 aagagctcca cgaccgagat cgccgccgcc gcgaccgcgg ccgccccgac ggcggcgaac
     1501 accgcccggc gtcgggtgcg cggcacctcg ggcaacccga acccgtacgc cgcgacgacg
     1561 agggccagcg cgtacccgag cgaccaggcg aagcgcgacg agccggtcat cgagtagccg
     1621 tgcgcgaccg cgtgcgcctt ccccagggcg aggacgcaca ccaccgtgcc ggcgttgacc
     1681 gccaggcgag ccatgcgccg catcggtcct ctatcggccg gtcggggcct ggtgctgagc
     1741 ccttccccac ccccgggggg gctagcggtg gtaccggctg acggccacgt cgatcccgga
     1801 gaccaccggg agggcctggc gggaccctcg gcggtagggc ccgaggcgtt ggggggcctc
     1861 tccgggggtg cggctcacca gcgtgaacga cggggcgtcg agacgtcgga cgacgtcgac
     1921 ggccgcgggc agctgggccg tggtctcggc ggtcagccac cggcggcccg gggtgtagaa
     1981 gccgccgccc tctcgccaga agtgcggcag gtcggtggtt accaccggtc cgggctccgc
     2041 cgccgcggcg ctgaccgcgc gggcgaacgc gtggctccga accgacagcc acgccaatcc
     2101 ggctccggtg atggtgccgg cgacgacgac aaccgcggcc gcgctgcgcc acggggccga
     2161 ctcgagcgcc accgcggcga cgacggcgag caacaacccg gacaggagga gataccggcc
     2221 a
//
LOCUS       DATCCH010000033         3471 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_543123, whole genome shotgun sequence.
ACCESSION   DATCCH010000033 DATCCH010000000
VERSION     DATCCH010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543123"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1212)
                     /locus_tag="VIJ44_00695"
     CDS             complement(<1..1212)
                     /locus_tag="VIJ44_00695"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY5164444.1"
                     /translation="MTCPACGAAAPVDARFCPTCGHALVARPDERRVVSVVFADLVGF
                     TSYSEFSDPEHVKALVDECFEALSADVIAYGGQVDKIVGDALLALFGAPVAHEDDAER
                     AVRCALRMQQTLAALREERDLTVELRVGVNTGVVLVGALRTGGDYTAMGDVVNTASRL
                     QTVAEPGRVVVGPDTHAATHHAVQYEPLGALTVRGRSEPVEAWLALSAPVPPGTRRRA
                     RTPLVGRDAEMGLLSAILDTALTRSRAHLVLLTGGAGVGKSRLASEVAARARDQHEAS
                     VFEGQCVPYGEDIWFPIAETIRTVCHLPANATHDAAREGVYAAVAKASGRPPDDPEVA
                     RTGSGLLYLLGYAEAFHDVDPGRARDDALRSGQALFGQLASERPVVLVLSDLHWADDL
                     VLDLVDRLLGAL"
     gene            complement(1222..2226)
                     /locus_tag="VIJ44_00700"
     CDS             complement(1222..2226)
                     /locus_tag="VIJ44_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011605348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY5164445.1"
                     /translation="MAQGTTIRVRWEPSPTATAASQLALFPTEERVVGRGEFRGLEFV
                     HVQARSIINRLPPATPMPFRHTINAYRGCGHACTYCFARPTHAYLDLDPSTDFDRVIV
                     VKVNAVERLRAELAPKRWAGDPVAMGTNTDPYQRAEGKYHLTRGIVEVLAEQRNPFSV
                     LTKSTLILRDRDLLADAARRTDVSLCFSVGTLDEDVWRLTEPGTPPPARRLDAVARLN
                     EAGIRTSVLIGPILPGLSDTPAQLAAVVRGAVDAGAASISSVLLHLRPGVRQHYLASL
                     REHRPDLVADHERLYARGAYAPREHRDRVSALVGDLEARHRRNHPTSTPVASGRFSGP
                     "
     gene            2312..2593
                     /locus_tag="VIJ44_00705"
     CDS             2312..2593
                     /locus_tag="VIJ44_00705"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006550586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEY5164446.1"
                     /translation="MAALSDAEIEAGLARRPGWERAGDELVRVYELESFRAVIALVDR
                     IADAAEAADHHPDLDIRYNRLRVVLSTHSEGGITALDFALAAEIDDLAR"
     gene            2594..3022
                     /locus_tag="VIJ44_00710"
     CDS             2594..3022
                     /locus_tag="VIJ44_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164447.1"
                     /translation="MALIVVGVVIVGLCVALVIIVGVEPGPSPADVALAYEEAWDHLD
                     FEGLWSLSGDELRDGLRRPDFVAAKRAAYEQQTGLRALADRVVVDAVSEGKGFAVVHT
                     RVELRDGGRAVDALQLAQRGGRWLVIAYDLEPDSAGPAAR"
     gene            3019..3435
                     /locus_tag="VIJ44_00715"
     CDS             3019..3435
                     /locus_tag="VIJ44_00715"
                     /inference="COORDINATES: protein motif:HMM:NF024093.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5164448.1"
                     /translation="MSGPVPDYVVVIVEDVGRSVDFYTQVLGLRLAHRSGPYAQLDTG
                     VTRVGLYERAAMAATLGRPLRSPDPDAPAFELGFKVDDCDAAYAALVAKGARGVVEPT
                     DRAWGQRTGYVVDPDGHLVELAEDLSGPTSPASAAP"
BASE COUNT          464 a         1101 c         1398 g          508 t
ORIGIN      
        1 caacgcgccg aggagccggt cgacgaggtc aaggacgagg tcgtcggccc agtgcagatc
       61 ggaaaggacg agcaccaccg gtcgttcgct ggcgagttgg ccgaagaggg cctggccgga
      121 gcgcagcgcg tcgtcgcggg cccggccagg atcgacatcg tggaacgctt cggcgtaccc
      181 gagaaggtag aggaggccgc ttccggtgcg ggccacctcg gggtcgtcgg gaggtcggcc
      241 gctggccttc gcgaccgcgg cgtagacgcc ttcgcgggcg gcgtcgtggg tggcgttcgc
      301 cgggaggtga cagacggtgc ggatcgtctc ggcgatcggg aaccagatgt cttcgccgta
      361 ggggacgcat tggccttcga agaccgacgc ctcgtgctgg tcgcgggcgc gggcggcgac
      421 ctcgctggcg aggcggctct tccccacgcc ggcgccgccg gtgaggagca ccaggtgcgc
      481 gcgcgagcgc gtcaacgcgg tgtcgaggat ggcgctgagc aaacccatct cggcgtcgcg
      541 gccgaccaac ggggtgcggg cccggcgacg cgttcccgga gggacggggg cgctgagcgc
      601 cagccaggcc tcgacgggct cggagcggcc ccgcaccgtg agggcaccga gcggctcgta
      661 ctggaccgcg tggtgcgtcg ccgcgtgcgt atcgggtccg acgacgaccc ggccgggttc
      721 ggcgacggtc tggagtcggc tggcggtgtt cacgacgtcg cccatcgcgg tgtagtcacc
      781 gccggtgcga agcgccccga cgagcacgac accggtgttg accccgacgc ggagctcgac
      841 ggtgaggtcg cgttcctcac ggagcgcggc aagggtctgt tgcatccgca gggcgcaccg
      901 cacggcgcgt tcggcgtcgt cttcgtgcgc gacgggggcg ccgaacagcg cgaggagggc
      961 gtcgccgacg atcttgtcga cctggccgcc gtaggcgatc acatcggcgg agagggcctc
     1021 gaagcactcg tcgacgaggg ctttgacgtg ttcggggtcg ctgaactccg agtaggaggt
     1081 gaagccgaca agatcggcga agacgacgct caccacgcga cgttcgtcgg gccgggcgac
     1141 gagggcgtgg ccgcaagtcg ggcagaaccg cgcgtcgacg ggtgccgccg ccccgcacgc
     1201 cgggcaggtc atggccgtag gttacgggcc gctgaaccgg cccgatgcga ccggggtcga
     1261 cgtcgggtgg ttgcggcggt gacgggcctc gaggtctccg acgagggcgc tgacccggtc
     1321 ccgatgctcc cggggggcgt aggcgccgcg cgcgtagagc cgttcgtggt cggcgacgag
     1381 gtcgggtcga tgctcccgga gcgacgcgag gtagtgctgg cgaaccccgg gtcgcagatg
     1441 gagcagcacc gatgagatcg acgcggctcc ggcgtcgacc gcgcctcgga cgacggcggc
     1501 cagctgggcg ggcgtgtcgg acaggcccgg gaggatgggg ccgatgagca cgctggtgcg
     1561 gatcccggcc tcgttgaggc gggccaccgc gtcgaggcgt cgcgccgggg gcggtgtccc
     1621 gggttcggtg aggcgccaca cgtcctcgtc gagggtgccg accgagaagc agagggagac
     1681 gtcggtgcgt cgggcggcgt cggcgagcag gtcccggtcg cgaaggatca gggtgctctt
     1741 ggtgaggacc gagaacgggt tgcgctgctc ggcgaggacc tcgacgatgc cgcgggtgag
     1801 gtggtacttg ccctcggcgc gctggtacgg gtcggtgttc gtgcccatcg ccactgggtc
     1861 accagcccag cgtttcgggg ccagctcggc tcggagccgc tcgacggcgt tgaccttgac
     1921 gacgatgact cggtcgaagt cggtgctcgg gtcgaggtcg aggtaggcgt gggtggggcg
     1981 ggcgaagcag tacgtgcagg cgtggccgca gccccgatag gcgttgatgg tgtgacggaa
     2041 tggcatcggg gtggccggcg ggagccggtt gatgatgctg cgggcctgga cgtggacgaa
     2101 ctcgaggccg cggaactcgc ctcggccgac gacgcgttcc tcggtgggga agagggcgag
     2161 ctgggacgcc gcggtcgcgg tcggtgacgg ctcccaccgc acccggatgg tggttccctg
     2221 ggccatcggc gcagggtagc gaacatacgt tcgtgaaggc aaggttcggt tccgcttggc
     2281 gtgttggtgc ggcggcgccg acaatggggt ggtggctgcc ctgtccgacg ctgagatcga
     2341 ggccgggctg gcgcgccgtc ccgggtggga gcgggccgga gacgaactgg tccgggtgta
     2401 cgagctggag tcctttcggg ccgtaatcgc cctggtggac cggatcgccg acgcggccga
     2461 ggccgccgat catcacccgg atctcgacat ccggtacaac cggctgcggg tggtgttgtc
     2521 cacgcacagc gaggggggca tcaccgccct ggacttcgcg ctggccgccg agatcgacga
     2581 cctggcgcgc tgagtggcgc tcatcgtcgt cggcgtcgtc atcgtcgggc tgtgcgtggc
     2641 gctggtgatc atcgtcggcg tggaacccgg cccgtcgccg gccgatgtcg cgctcgcgta
     2701 cgaggaggcg tgggatcacc tcgacttcga agggttgtgg tcgctgtccg gggacgagct
     2761 gcgcgacggt ctacgccggc cggatttcgt cgccgcgaaa cgggccgcgt acgagcagca
     2821 gaccggtctg cgtgccctcg cggaccgggt cgtcgtcgac gcggtcagcg aagggaaggg
     2881 cttcgcggtc gtgcacaccc gggtcgagct ccgagacggc ggccgggccg tcgacgcgct
     2941 ccaactcgcg cagcggggtg ggcggtggct ggtgattgcc tacgacctcg agcccgacag
     3001 cgccggcccc gcggcgcggt gagcggtccg gttccggact acgtggtggt gatcgtcgag
     3061 gacgtcggcc ggtcggtgga cttctacacc caggtgctcg ggctgcgcct cgcgcaccgt
     3121 tccggtccgt acgcgcagct cgacaccggc gtgacccggg ttgggctcta cgagcgggcc
     3181 gcgatggccg ccacgctcgg tcggccgttg cgatcccccg atccggacgc gccggcgttc
     3241 gagctcggct tcaaggtcga cgactgcgac gcggcgtacg ccgcgctggt cgccaagggc
     3301 gcgcggggtg tcgtcgagcc gaccgaccgg gcatgggggc agcgcaccgg gtacgtcgtc
     3361 gatcccgacg ggcatctggt cgagctcgcc gaggatctca gcgggccgac cagccctgcc
     3421 agcgctgcac cgtgaccacg atcgccggcc ccgccggtgg cacctcggcg t
//
LOCUS       DATCCH010000034         7810 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694 k141_2029,
            whole genome shotgun sequence.
ACCESSION   DATCCH010000034 DATCCH010000000
VERSION     DATCCH010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7810
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2029"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="VIJ44_00720"
     CDS             <1..767
                     /locus_tag="VIJ44_00720"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY5164449.1"
                     /translation="RVAIVGGGANAAAACADGGFLVTQNGGIDFASTNLYPEDPPPYR
                     PVTPGIQRVSADGRVAYVTDSGYRAPNDLVVTADGTLWFTDPPPFPPSGEPVGRVHTM
                     QPDGTSRVEAAGFMYCNGIALEPDGTPVVVEARGLVRLEPDGGRHWVVETIGASASAG
                     DGLCVDAEGRLYVANTAEHGVRVYEPDGHEVEFLAIPGRGVTTNCCFGGRDRRTLFAT
                     DGLPGNVVAWEGLSTPGLPLHPWPHPDTFAATARAT"
     gene            782..1858
                     /locus_tag="VIJ44_00725"
     CDS             782..1858
                     /locus_tag="VIJ44_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018637247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HEY5164450.1"
                     /translation="MRAAIFVEQDGDLVVEDVKATDPGPRDVVVRITASGVCHSDLSV
                     INGTLPMPPPAILGHEGTGIVEDVGAEVSRVKPGDRVIGSFIPACGVCWYCRNQHSNL
                     CEQTYAVMGTPRATRKDGTSLPSMTGLGTFADVMTCDEISLVKVETDLPDEQLALIGC
                     GVTTGVGAALNTAQVVPGSTVAVVGCGGVGQAVIQGAKIAGAARIIAVDPVELKRKTA
                     LQLGATDTVDPSAGNATAQVQELTGGRGADYAFEVIGNPVTVQQAFDMARPGGACVVV
                     GVPRFDQTIELPSMPLVLQEKRVLGTVYGSAQVNRDFPRLIGLVEQGRLDLGHMVSRR
                     MKLDDINDAFKAMQAGEVIRSVIS"
     gene            complement(1870..2595)
                     /locus_tag="VIJ44_00730"
     CDS             complement(1870..2595)
                     /locus_tag="VIJ44_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017624166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEY5164451.1"
                     /translation="MAVAPDLTIERILVITAHPDDVDFGVAGSVATWTDAGIEVTYCL
                     VTDGEAGGSDRSLSRTEMAELRRAEQTAAAKVVGVEDLVFLGHPDGRLEPTLDLRRDL
                     SRVIRQVRPQRVVTQSPDRNYERIYASHPDHLAAGEAALCAVYPDARNPFAHPELLED
                     ESLEPWSVGETWLMAAGTPNRFVDVTDTFDRKIDALRSHASQHSDWDPIAEMIRGWLS
                     ATAKLATFPDGRLAEGFYGLETG"
     gene            complement(2639..3139)
                     /locus_tag="VIJ44_00735"
     CDS             complement(2639..3139)
                     /locus_tag="VIJ44_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012950200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5164452.1"
                     /translation="MSGAQVNSSSREQEWADLRRQYLCPKGERPATTARGVHHVALLS
                     QDVEQTIRFYQDLLGFPLTDLFENRDYPGSTHFFFDIGNGNALAFFDFPSLDLGGYAE
                     VLGGLHHLAISVPPDRWAELRMRLDAAGVEMERIDSSLYFRDPDGARVELISDPLGDL
                     YGNPVL"
     gene            complement(3136..4314)
                     /locus_tag="VIJ44_00740"
     CDS             complement(3136..4314)
                     /locus_tag="VIJ44_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013425887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5164453.1"
                     /translation="MDLDYLAFDADNHYYEALDAFTRHLDPGLGPRCVQWAEVNGRHY
                     HVVGGRVSHAVVNPTFDPVAKAGALHDYFRGNPEGKSPLEFLRAREPIRADYRDRDAR
                     LATLDEFGLESIWLFPTLGILYEELLKHDPEAVTLTFTAFNRWLDEDWGVNYQDRIYA
                     APYLSLCDVDWAVRELDWALERDARVVVMRPAAPTTKVGQLPPTHPTFDPFWARANEA
                     GITVVIHAGDSGYSSQGYASDGFAATFEGAGRPSIRMLAIERAAYDFLLTLVFDQLFD
                     RFPNLRVASVENGSEFLPDLFHKLGSAQRKMPGYFRDDPADQFRRHVWINPFWEDDPY
                     QIIDLMGSDRVIFGSDWPHIEGIPRPLDYVNELKELDDADRRLVLRDNAIELTRRRPA
                     "
     gene            4433..5560
                     /locus_tag="VIJ44_00745"
     CDS             4433..5560
                     /locus_tag="VIJ44_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007536000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEY5164454.1"
                     /translation="MIEPGDDGPETLGRPQTSTRDHNAVRAQLQAWLALQFPAGASPR
                     ITGFEIPASNGLSSETVLVDVESDRDEQPSTDRLVVRLAPPASAVPVFPVYDLDRQAK
                     VMRLVAAHTSVPVPRVRWSEPDPRPLGSPFFVMDRVDGLVPPDLLPYNFGSWLSEADP
                     TDQRRLQDATVAILAALHNIDATTHFSFLRLEPGSALAAHIADQRAYYTWVADGLRIP
                     LLEECFAWLEDHCPAAEGPAAFSWGDSRIGNIMYRDFEPVAVLDWEMAGLGPPELDLG
                     WLIFMHHFFEDVAAQFSLPGMPGFLRPDDVATTYESLTGHAPRDLGFYMLYAALRHGI
                     IMSRIQRRAIHFGEATMPDDPNDLISHRATVEAMLAGTYWR"
     gene            5852..6679
                     /locus_tag="VIJ44_00750"
     CDS             5852..6679
                     /locus_tag="VIJ44_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164455.1"
                     /translation="MGIDENNRPFSAEPRELSADFNAIKASSAEWVSFDFDWSTVQAA
                     GPTSYSWAPLDKAVDAANARGLKVLAIPDYTPSWARAPRTDNKYPPTNNGDYVNFVAA
                     AVTRYAPQGVHTWAIWNEPNNPDFWKPNPDVAAYSQLLKATYPTIKTLDRSAVVLTGG
                     TAPASGSLSPASWLSGLYAHGAKDSFDAISHHPYAGLPYGPATVAPWNPFQQTLELHH
                     LMVANADGAKKIWGTEAGAYTGEAIGAVDRTTQAQFITDYLRLWSRWSSFTGPLFIY"
     gene            6861..7535
                     /locus_tag="VIJ44_00755"
     CDS             6861..7535
                     /locus_tag="VIJ44_00755"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HEY5164456.1"
                     /translation="MTTAELYPAVRAKLLELAAGLSREQSELPVPALPGWRVKDAYAH
                     LTGLCGDMLDGRMEGAGSAAWTAKQVAARSRHDLSEICAEWLERGPALDDQLAKAGDH
                     APTFVAFDVWNHYQDVRSAVGLRGERDREQLAYLAAEALGAFDGRFREAGVPALRVIT
                     EHVDRNLGDGPPDATLATTDYELVRILFGRRSLAQITNAGWNGEPAAYVDHLHLFDPP
                     VSDLAD"
BASE COUNT         1278 a         2809 c         2472 g         1251 t
ORIGIN      
        1 agcgggtcgc catcgtcggt ggtggcgcca acgccgctgc ggcgtgtgcc gacggcggtt
       61 tcctcgtcac ccagaacggt gggatcgact tcgcgagcac caacctgtat cccgaggacc
      121 caccgcctta ccgtccggtc acgcccggca tccagcgggt cagcgccgac ggtcgcgtcg
      181 cgtatgtgac cgattcgggc taccgcgccc cgaatgacct cgtcgtcacc gccgatggca
      241 cgctgtggtt caccgacccg ccgccgttcc ctccgtccgg tgaaccagtc gggcgggtcc
      301 acaccatgca gcccgacggg accagccgcg tcgaggccgc gggattcatg tactgcaacg
      361 gcatcgccct cgaaccggat ggcaccccgg tcgtcgtcga agcccgaggg ctggtccgcc
      421 tcgagcctga tggaggtcgc cattgggtcg tggagacaat cggcgccagc gccagcgccg
      481 gcgacggcct ctgcgtcgac gccgaaggcc gtctctatgt cgcgaacacc gccgagcacg
      541 gtgtgcgcgt gtacgaaccc gacgggcacg aggtcgagtt cctcgccatc cccggccgcg
      601 gcgtcaccac caactgctgc ttcggcggcc gcgaccgccg caccctcttc gccaccgacg
      661 gcctacccgg gaacgtggtc gcctgggagg gcctctctac acccggcctc ccgctgcacc
      721 cctggccgca ccctgatacg ttcgccgcga ccgctcgggc aacttagagg gagacccccg
      781 catgcgtgca gccatcttcg tggagcaaga cggcgaccta gtcgtcgagg acgtcaaggc
      841 caccgatccc ggtccccgcg acgtcgtcgt ccgcatcacc gccagcggcg tctgccactc
      901 cgatctctcg gtcatcaacg ggaccctgcc gatgccgccg ccggcgatcc tcggccatga
      961 agggaccggc atcgtcgagg acgtcggtgc cgaggtctcc cgggtcaagc ccggcgaccg
     1021 ggtcatcggc tcgttcatcc cggcctgcgg cgtctgctgg tactgccgca accagcactc
     1081 caacctctgc gagcagacct acgcggtgat gggcacaccc cgcgccactc gcaaagacgg
     1141 cacctcgctc ccgagcatga ccggtctcgg tacgttcgcc gacgtcatga cctgcgatga
     1201 gatctcgctg gtcaaggtcg agaccgacct tcccgacgag cagctcgctc tcatcggctg
     1261 cggggtgacc accggggttg gcgccgccct gaacacggcg caggtcgtgc cgggctcgac
     1321 ggtcgccgtc gtcgggtgcg ggggggtcgg ccaggccgtc atccaagggg ccaagatcgc
     1381 cggcgcggcg cgcatcatcg cggttgaccc ggtcgagctg aagcgcaaga ccgccttgca
     1441 gctcggcgcg accgacaccg tggaccccag cgcggggaac gcgaccgccc aggtccaaga
     1501 gctgaccggc gggcgcggcg ccgactacgc gttcgaggtg atcggcaacc cggtgacggt
     1561 gcagcaggcg ttcgacatgg cccgccccgg gggggcctgc gtggtcgtgg gtgtgccccg
     1621 cttcgatcag accatcgagc ttccgagcat gccgttggtg ctccaagaga agcgggtcct
     1681 cggaaccgtg tacggctccg cgcaggtcaa ccgcgacttc ccacgtctca tcggtctcgt
     1741 cgagcaaggt cgcctcgacc ttggtcacat ggtctcgcgc cggatgaagc tcgacgacat
     1801 caacgacgcg ttcaaggcca tgcaggccgg cgaggtcatc cgcagcgtca tcagctgacg
     1861 ccgaggggtt cagccggtct cgagcccgta gaacccttca gccagtcgcc cgtcggggaa
     1921 tgtcgccagt ttcgcggtcg cgctcagcca gccccggatc atctcggcga tcggatccca
     1981 gtcgctgtgc tggctggcgt ggctgcgcaa cgcgtcgatc ttgcgatcga aggtgtcggt
     2041 gacgtcgacg aagcggttcg gcgttcctgc cgccatgagc caggtctcgc cgaccgacca
     2101 cggctccagg ctctcgtcct cgagcagctc ggggtgcgcg aacgggttac gggcgtcggg
     2161 gtacaccgcg cacagcgcgg cttcccccgc cgcgaggtga tcagggtggc tggcgtagat
     2221 ccgctcgtag ttccgatccg gggactgggt gaccacccgc tgagggcgaa cctgacggat
     2281 cacccggctg aggtcacggc gcaggtccag cgtcggctcc agccgaccgt cgggatgacc
     2341 cagaaacacc aggtcttcga caccgaccac cttcgccgcc gcggtctgct cggcccgtcg
     2401 gagctcggcc atctccgttc gcgacaagct gcgatccgag ccacccgctt ccccgtcggt
     2461 cacgaggcag tacgtgacct cgattcccgc gtcggtccac gtcgccacgc tgcccgccac
     2521 gccgaagtcg acgtcgtcag gatgggccgt gatcaccagg atccgctcga tagtcaggtc
     2581 cggtgccacg gccagcgacc ctagtccccc gcctgatcga cgccgcttgg cgcgcgcatc
     2641 agaggaccgg gttgccgtac agatcgccga gcgggtcgct gatcagctcc actcgcgccc
     2701 cgtcggggtc acggaagtac agcgacgagt cgatgcgctc catctccaca ccggcggcgt
     2761 cgagtcgcat gcgcagctct gcccatcggt ccggagggac cgagatggcg aggtgatgga
     2821 gtccgccaag cacctcggcg tagccgccga gatcgaggct cgggaagtcg aagaaggcca
     2881 gggcgttgcc gttcccgatg tcgaagaaga agtgggtcga gccggggtag tcacggttct
     2941 cgaaaagatc ggtcaagggg aagccgagca gatcctggta gaagcgaatc gtctgttcga
     3001 cgtcctggct caggagcgca acgtggtgga cgccgcgtgc agtcgtcgcc ggccgctcac
     3061 ccttggggca cagatactgg cgtcggaggt cggcccactc ctgctcccgc gagctggagt
     3121 tgacctgagc gccgctcacg caggccgccg tcgagtgagc tcaatcgcgt tgtcgcgcag
     3181 caccagccgc cgatccgcgt cgtcgagctc cttgagctcg ttcacgtagt ccaacgggcg
     3241 cgggatcccc tcgatgtggg gccagtccga cccaaagatg acccgatccg atcccatcag
     3301 gtcgatgatc tggtacgggt cgtcctccca gaacgggttg atccacacgt gacgtcggaa
     3361 ctggtccgcc gggtcgtccc ggaagtagcc gggcatcttg cgctgcgcgg agccgagctt
     3421 gtggaacagg tcgggcagga actccgagcc attttcgacc gaagcgaccc gcaggttcgg
     3481 gaaacggtcg aacagctggt cgaataccag cgtgaggaga aagtcgtacg ccgcccgctc
     3541 gatggccaac atcctgatcg acggacggcc ggctccctcg aacgtcgccg cgaacccgtc
     3601 gctcgcgtag ccctgtgagg agtagccgct gtcaccggcg tggatcacga cggtgatgcc
     3661 ggcctcgttg gccctggccc agaacgggtc gaacgtgggg tgtgtcggtg gcagctgccc
     3721 gaccttggtg gtcggcgccg ccgggcgcat cacgacgaca cgcgcgtctc gttcgagtgc
     3781 ccagtcgagc tcgcggaccg cccagtcgac atcgcagagc gagaggtacg gggcggcgta
     3841 gatccgatcc tggtagttca cgccccagtc ctcgtcgagc caccggttga acgctgtgaa
     3901 cgtcaacgtc accgcttcgg ggtcgtgttt cagcagctct tcgtagagga tcccgagcgt
     3961 cgggaacagc cagatggatt ccaatccgaa ctcgtcgagg gtggccaggc gagcgtcgcg
     4021 gtctcggtag tcggcgcgga tcggctcccg ggcccgaaga aactcgaggg gcgacttccc
     4081 ttcggggttc ccccggaagt agtcgtggag cgcaccggcc ttcgccaccg ggtcgaacgt
     4141 cgggttgacg acggcgtggc tgacccggcc tccgacgacg tgatagtgcc gcccgttgac
     4201 ttcggcccac tggacgcacc tcggcccgag gccgggatcg agatgacggg tgaaggcgtc
     4261 gagtgcctcg tagtagtggt tgtccgcatc gaaggccaaa tagtcgaggt ccacggcgac
     4321 cagcgtagag gagccaccat gggtggtgcg gtggggcggc gcggaagccg gccgcccacg
     4381 accacgggct ggaaccgtcc cccgtcgact cgactggtag acacgagggc ccatgatcga
     4441 accaggtgat gacggacccg agacgctcgg acgccctcag acctcgaccc gtgatcacaa
     4501 cgcggtgcgc gcccagctgc aggcctggct ggcgctccag tttccggccg gcgcttcgcc
     4561 ccggatcacc ggcttcgaga tccccgcgtc gaacgggctt tcgagcgaga ctgtcctcgt
     4621 tgacgtcgag tcggaccgcg acgagcagcc gagcaccgac cgtctggttg ttcggctcgc
     4681 cccaccggca tccgcggtgc cggtctttcc cgtatacgac ctcgaccgcc aggcgaaggt
     4741 catgcggctc gtcgccgccc acacctccgt gcccgtcccc cgggtccgct ggtctgagcc
     4801 cgacccccgt cctctgggct cgccgttctt tgtcatggac cgtgtcgacg gtctcgttcc
     4861 ccccgacctg ctgccctaca acttcggcag ctggctcagc gaggctgacc ccactgatca
     4921 acgccggctt caagacgcca ccgtcgcgat cctcgccgcg cttcacaaca tcgacgcgac
     4981 gacccacttc tcgttcctcc gtctcgaacc cggctcggcg ctcgccgcgc acatcgccga
     5041 ccagcgcgcc tactacacgt gggtggcgga cggcctgcgg atcccactcc tcgaggaatg
     5101 cttcgcctgg ctcgaggacc actgccccgc cgccgagggt ccagccgcgt tcagctgggg
     5161 cgattcgcgc atcggcaaca tcatgtatcg cgacttcgag ccggtcgccg tcctcgactg
     5221 ggagatggcc ggtctcggac ctcccgagct cgacctcgga tggctcatct tcatgcatca
     5281 cttcttcgaa gacgtcgccg cccagttcag cctccctggc atgcccgggt tccttcggcc
     5341 cgacgatgtc gccacgacct acgagtccct cactggccat gctccgcggg acctcggctt
     5401 ctacatgctg tatgccgcgt tgcggcacgg catcatcatg agtcgcatcc aacgtcgcgc
     5461 catccatttc ggtgaggcga ccatgcccga cgaccccaac gacctcattt cgcatcgcgc
     5521 gaccgtcgag gcgatgctgg caggcaccta ttggcgctga tgcgtcgacg tcggggactc
     5581 actgtctggt tcgggcgcgc cgaccgtccg ttcggggctc cgaacagctt ctccgaccgt
     5641 tccaggccgg gcccccgtgg ctgaccgcgt cgatctgtct gatccggccg cctggctcac
     5701 cgcgccgggg acccgcacct ggctgatcct cgctctggtt ggagcagtgc tgtttgtgag
     5761 gccggggcca ttcgccccca ccaccacgac gcacgaccct gtcaccagcc ggcaaccgtc
     5821 ggcgcctcgc aacactgtcg ccgcttcgcg cgtgggcatc gacgagaaca accgcccgtt
     5881 ctccgcggag ccccgggaac tcagcgccga cttcaacgcg atcaaggctt cgagtgcaga
     5941 gtgggtctca ttcgacttcg actggtccac cgtccaagcc gcaggaccaa cgtcgtacag
     6001 ctgggccccg ctggacaagg cggtcgatgc cgccaacgcg cgcggcctca aagtactcgc
     6061 catccccgac tacaccccct cctgggctcg tgcccccagg acagacaaca aatacccgcc
     6121 aacgaacaac ggcgactacg tcaacttcgt tgccgccgcg gtgacccggt acgccccaca
     6181 aggagtgcac acgtgggcga tctggaacga acccaacaac cccgactttt ggaaacccaa
     6241 cccggacgtc gccgcgtaca gccaactcct gaaagccaca tatccgacga tcaagaccct
     6301 cgaccgatca gccgtcgtcc tcaccggcgg gaccgcgccc gccagcggct cactctcacc
     6361 cgcgagctgg ctgtcaggcc tctacgctca cggcgccaaa gactccttcg atgccatcag
     6421 ccaccacccc tacgccgggc tcccctacgg acctgccacc gtcgcccctt ggaacccgtt
     6481 ccaacagacc ctcgagctgc accacctcat ggtcgccaac gccgacggcg ccaagaagat
     6541 ctggggcacc gaagccggcg cgtatacggg cgaggccatc ggggctgtcg accgaacaac
     6601 ccaagcccaa ttcatcaccg actaccttcg gctttggagc cgatggtcca gcttcaccgg
     6661 accgctcttc atctactaac tgcgtgacgc cggcaccaac cgcgccaacc gcgaggacaa
     6721 ctggggactg ctcaccacaa atggcacagc caagccggca tggcacgcct tcatcgccgc
     6781 cctcggccgg taacgtcacc cccgagcgcc cagccgttcg gaccgaactc gcggtcgacg
     6841 cggtcccgta tcatgccgac gtgacgaccg ccgagctgta ccccgcggtc cgagccaagt
     6901 tgctcgagct cgccgccggc ctttcccgcg agcagtccga gttgccggta ccagccctgc
     6961 cgggctggag ggtcaaagac gcgtacgcac acctgactgg tctctgcggc gacatgctcg
     7021 acggtcgcat ggaaggggcc gggtctgccg cctggacggc gaaacaggtc gcggcccgct
     7081 cacgccacga tctctccgag atctgcgcgg aatggctcga acgtggcccc gccttggacg
     7141 accagctcgc gaaggcgggg gatcacgccc cgacctttgt cgccttcgac gtttggaacc
     7201 actatcaaga cgtgcgatcc gccgtcggcc tgcgcggtga acgagaccgc gaacagcttg
     7261 cctacctagc cgccgaggcc ttgggcgcct tcgacggtcg gttccgcgag gccggagtgc
     7321 ccgccctgcg ggtgatcact gaacacgtcg accggaatct cggcgacggg ccgcctgacg
     7381 ccaccctcgc cacgactgac tacgagctcg tgcgcatcct gttcggccgg cgaagcctcg
     7441 cccagatcac gaacgcaggt tggaacggcg agccagcggc atacgtcgac cacttgcact
     7501 tgttcgatcc tccggtgagc gacctcgccg actaggcgtg cggtccgcgg tccccgtcag
     7561 tcgaggcgat caggtgatcg ccgccgcctg tccgctccag cagccaatcg tcgacgtgaa
     7621 ggacccagtg atccggctca tcgacggtcg gcgggcggta tgaagcggcc gtgaaggcct
     7681 cccgcgacgc gcagcgcgaa gctggcgtcc tcagtgatgt cgtcctcgac ccggacgtcg
     7741 ccatgccggg cgagaagcgg aagggcggtg atcatctcgg ccatggccag gtgctgtccg
     7801 atgcatcccc
//
LOCUS       DATCCH010000035         2090 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_87490, whole genome shotgun sequence.
ACCESSION   DATCCH010000035 DATCCH010000000
VERSION     DATCCH010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87490"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(294..1835)
                     /locus_tag="VIJ44_00760"
     CDS             complement(294..1835)
                     /locus_tag="VIJ44_00760"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY5164457.1"
                     /translation="MTAKSVAPGQHAPLWPPADSGRQAIQRSPQGPTRRVPKAARHDR
                     SYAIPKTAYVSTYAPHRCGIATFTYDLATTVGEREIVALHPADEPGPYPAEVRHRIRR
                     DVQADYGSVARALNDCGVDVVSIQHEYGIWGGDDGAYVLDFVRALRMPAVATLHTVLR
                     HPTLSQRRILTELIDASTATVVMSRAAASLLTRAYAVEPTRLNIVAHGVPDLPLVAPD
                     TVKPRLGLKGRQVILSFGLLGPGKGYESAIAAMPAVVKAMPSASYVILGATHPDLLHR
                     EGEAYRRRLEATAAALGVTDHVQFVDRFVGRVELGTWLEAADIFVTPYPNLDQIVSGT
                     LSYAMGAGKAIVSTPYAYALERLAHGRGRLVPAGSPDALAETFIELLRDPELRASLGR
                     RAYEYSRGMVWSEVGAEYRRVFARAARSAPVPGINANLTAGLDLTRARRLRENLGIAF
                     QGPVKVGPFEIDGPTALKMLASPGGPTAPDEDIAPTTRLSDGSRSDGSGRSGAGHREA
                     TRRWD"
BASE COUNT          329 a          704 c          760 g          297 t
ORIGIN      
        1 ggccattccg ggtagagctg cgtcggcgcg tcctcccacc cctgagaccg gggcgcgcga
       61 cagcggataa ctgcggtcga aatggagggg tcgccacgag gggagccggg gtgctgggag
      121 gctcagactt gggaagcaga cactctgcca gctgagttac tcccgctcgg gaggacccga
      181 ttgtagcgga ccgccgtcgc gacgccaacg cgctgctcgt gccatccgac caaggccggg
      241 gctcgccgtc gcccgcgcat acgacggcca gcacggctgc gccgctcgca cggtcagtcc
      301 caccggcgtg tcgcctcccg gtgacccgca ccggacctgc cagacccgtc tgatcgcgac
      361 ccgtcggaca ggcgcgttgt gggcgcgatg tcctcatcgg gagcggtagg cccaccgggc
      421 gacgccagca tcttgagggc cgtgggcccg tcgatctcga acgggccgac cttcacaggg
      481 ccctggaacg cgatgccgag gttctcccgc aatcgccggg cccgagtcag atcgaggccc
      541 gcggtgaggt tcgcgttaat gccgggtacg ggggccgacc gggcggcgcg ggcgaagacc
      601 cgacggtact ccgctcccac ctcggaccag accatgccgc ggctgtactc gtaggcgcgc
      661 cggccgagcg aggcgcgaag ctctggatct cgcagtagct cgatgaaggt ctcggcgagc
      721 gcgtccgggg agcctgctgg aacgagcctg ccgcgcccgt gcgccagccg ttcgagggcg
      781 tatgcgtagg gtgtcgagac gatcgccttc ccggcaccca tggcgtacga gagcgtgccg
      841 gatacgatct ggtcgaggtt cgggtagggc gtgacgaaga tgtcggcggc ctcgagccag
      901 gtcccgagct cgactcgacc gacaaagcgg tcgacgaact ggacgtggtc ggtcacaccg
      961 agagcggccg ctgtggcctc gagtctccga cggtacgcct cgccctcgcg gtggagcagg
     1021 tccgggtgtg tcgcgccgag gatcacgtag gacgccgagg gcatggcctt gacgacggcc
     1081 ggcatcgccg cgatcgccga ctcgtagccc ttgccgggcc ccagcaaccc gaagctcagg
     1141 atcacctgac gacccttgag gcccaggcga ggtttcacgg tgtccggcgc gacgagcggc
     1201 agatcgggga cgccgtgagc gacgatgttg aggcgggtcg gctcgacggc ataggcgcga
     1261 gtcagcaacg atgccgccgc ccgagacata accacggtcg cggtcgacgc atcgatcagc
     1321 tcggtgagga tccgccgctg cgagagggtg gggtgccgga gcaccgtatg gagcgtcgcc
     1381 acggcaggca tccggagcgc ccggacgaag tcgagcacgt acgcgccgtc gtcaccgccc
     1441 cagatgccgt actcgtgttg gatcgagacg acgtccacgc cacagtcgtt gagcgctcgg
     1501 gcgacggacc cgtagtcggc ctgcacgtcg cgccggatgc ggtggcggac ttccgctgga
     1561 tacgggcccg gctcatcggc cgggtggagg gcgacgatct cgcgctctcc gaccgtcgtg
     1621 gcgagatcgt aggtgaaggt cgcgatcccg catcggtgtg gcgcataggt agagacgtag
     1681 gcggttttcg ggatcgcgta gctcctgtcg tggcgcgccg ccttcgggac acggcgcgtt
     1741 gggccctgtg gcgatcgctg gatcgcctgg cgtcccgagt cggcgggcgg ccagagggga
     1801 gcatgctggc cgggcgcgac cgacttcgcg gtcatcgtcc tggatcgacg cgcgatcggg
     1861 ccccacaata ccagacgccc cacgaactcg cgctccgccc tcaccgccaa cgacgcctcc
     1921 tcgacgtcgg ggcgttgcgc cacgggctgg ctggcccgcg agatgcaaca cgatgaccac
     1981 gtcgtgtccg gccggcgcca ggccctgggt caagaggacc aagaggtctg cggcaacgcc
     2041 gtaacgcccc cacggggcaa cgcggcgcca cgtgcaaccg acagtcggat
//
LOCUS       DATCCH010000036         5367 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_375577, whole genome shotgun sequence.
ACCESSION   DATCCH010000036 DATCCH010000000
VERSION     DATCCH010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5367
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375577"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VIJ44_00765"
     CDS             <1..573
                     /locus_tag="VIJ44_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014670535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5164458.1"
                     /translation="FGVVGPVERVALDDARRCMETNLFGAARVIQAVLPGMRERGTGT
                     IVNVSSVAGRVGVPLDGFYSASKWALEGLSETLHFEVAHFGIRVRIVEPGFFETEFHG
                     NTVRHGLDDAPYDELDRQWQTARDVLNGDGEPQGADVVAGIIADAVESTEARLRWPVG
                     NDADLAISTKDSRSFEDFESTMRDLLGLAW"
     gene            570..1292
                     /locus_tag="VIJ44_00770"
     CDS             570..1292
                     /locus_tag="VIJ44_00770"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEY5164459.1"
                     /translation="MNEPNGETPGSALAVYAHPDDPEISAGGTLARWADAGAQVWVLI
                     TTRGDKGSPDPDVDSVALTRLRVEETAKAGALLGFADHFHLDYGDGELPDDLNLRAAI
                     VRHVRDLRPEVVLCPDPTAVFFGDAYFNHRDHRATGWATLDAVAPAAGNPHYFRDQIV
                     AGVPTHHVRAVYLSGTLEPNCWVDISDVLERKIDALFCHASQLTETGEWFREFLRERA
                     EDAGRTAGVRYAEAFRRLTFGS"
     gene            complement(1293..1571)
                     /locus_tag="VIJ44_00775"
     CDS             complement(1293..1571)
                     /locus_tag="VIJ44_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164460.1"
                     /translation="MAGDDDELDTGGVPAGLPSRGAYLLAFGSVVLAGVFGGIIGYGL
                     VDVGCTGNCSTSKVVGTLAGALTGAIGVGIVAVLVLRAMAEWKRRPRG"
     gene            1616..2125
                     /locus_tag="VIJ44_00780"
     CDS             1616..2125
                     /locus_tag="VIJ44_00780"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY5164461.1"
                     /translation="MREWLVAGALVEIDGALLLVRNERRGGHSDWTTPGGVIDADDQS
                     LLDGLAREVYEETGLRVTAWEGPLYEVNAVARDMDWRLRVEVHRAIEFEGELRVADPD
                     GIVVEAEFIATPGCLARLAGGAPWVREPLVDWLAERWGPDAARGYSYEVRGSRREDLS
                     VERTLPMTG"
     gene            2122..3321
                     /locus_tag="VIJ44_00785"
     CDS             2122..3321
                     /locus_tag="VIJ44_00785"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF003015.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEY5164462.1"
                     /translation="MSVVSEPGILHVDLDAFYASVEQGADPSLQGRPVVVGGLGPRGV
                     VAASSYEARQFGIQSAMPMARARRACPDAVFLAPRFDAYAAASRAVMAILRSYTPLVE
                     PISLDEAFLDARGARRLHGPARTIALEIRARVRAETGLTASVGLATTKLLAKIASDLG
                     KPDGFLIVEPGTELDFLHPLDVGRLWGVGPATRRRLERLGAHTIGDLAVIPESTLCHT
                     LGDAHGSHLHSLAHNDDSRPVEPERHAKSIGHEETFSTDVTDRHRLEVDLMRFADRVA
                     TRLREGAVAARTVQLKARYADFRTITRSRTLPDATDVAQDLVTVGRELLREIDLEDGL
                     RLLGLSAQQLVPITEVQPRLPLGVRTDVSRHASLERTIDEVRARYGDDAVTRAPRAPA
                     GPERSRP"
     gene            3318..4379
                     /locus_tag="VIJ44_00790"
     CDS             3318..4379
                     /locus_tag="VIJ44_00790"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY5164463.1"
                     /translation="MTVRVGIVGCGHIGFIHSYTLHQLMKHGLVDACVTATYDVVPER
                     AATFALAHGAVACDNVDAVLDQVDAVWVCTWTAAHHDVVAAAATRGLAIFCEKPLAPT
                     FPECEAVAQILATVPHQVGLVLRHAPVFQALVDEVTTGRHGRPLAVVFRDDQYFPVQG
                     VYASEWRADVARAGGGALFEHSIHDVDVLRWLLGDPTTVRADVASRYQHPGIDDVAAL
                     TLTFADDTIATLTSVWHQILSRGSTRRVEVFCDDALVWLDDDHLGPLHVESSDRSEII
                     EPEAPPWVGELAVPPALTRMVGEYAPATKGFLDALAAHGTAATGFPDAATALAAHRVV
                     DAAYRSAAAGGEAITPPPR"
     gene            4475..4858
                     /locus_tag="VIJ44_00795"
     CDS             4475..4858
                     /locus_tag="VIJ44_00795"
                     /inference="COORDINATES: protein motif:HMM:NF022684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3040 domain-containing protein"
                     /protein_id="HEY5164464.1"
                     /translation="MPLSEDEHRILKEIEANLSVTDPKLVQQVSDTTLYRHAAGMIKW
                     AVAGFVVGLILLIATFTSVLALGVVGFLIMLFCLLVIERNVRKMGRAGLESLTGSLRG
                     GALKGAFGDAGRRWRERWHRDDDPS"
     gene            4806..5321
                     /locus_tag="VIJ44_00800"
     CDS             4806..5321
                     /locus_tag="VIJ44_00800"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY5164465.1"
                     /translation="MPAAGGANAGTATTTRPEPLSARTGFSAPQAARLSGCSRSQLEY
                     WQRCGLIVAHPDASDGPYSFRDLVALRMVRSLLDAGLPLARIRRAVAAVIDAGEDMAG
                     LRMVTDGDHVWACRDDGQILDALRHGQLALFVAVDQMAADVDTEVRAFSQDRQTFVDR
                     LRGSDHPAGSG"
BASE COUNT          776 a         2050 c         1748 g          793 t
ORIGIN      
        1 ttcggcgtcg tcggccccgt cgaacgggtc gccctcgacg atgcccggcg ctgcatggag
       61 accaacctgt tcggcgcggc gcgcgtgatc caggcagttc tgcccgggat gcgcgagcgc
      121 ggcaccggga ccatcgtcaa cgtctcgtcc gtggcaggtc gcgtcggcgt tccgctcgac
      181 ggcttctact cggcctccaa atgggcactg gagggcctat ccgagaccct gcacttcgag
      241 gtcgcacact tcggcatccg ggtgcggatc gtcgagccag gtttcttcga gaccgagttt
      301 cacggcaaca ccgtgcgcca cggactcgac gacgctccct acgacgagct ggaccgccag
      361 tggcagacag cgcgcgacgt gctcaacggt gatggcgaac cccagggcgc cgacgtcgtc
      421 gccgggatca tcgccgacgc cgtcgaaagc accgaggcgc gactgcgctg gcccgtcggc
      481 aacgacgccg acctggcgat cagcaccaag gactcgcggt cgttcgagga cttcgagtcg
      541 acgatgcggg acctgctcgg cctggcgtgg tgaacgaacc caacggcgag acgccgggca
      601 gcgcgctcgc cgtctacgcg caccctgacg atcccgagat ctccgccggc ggcaccctgg
      661 cccggtgggc cgacgcgggc gcccaggtct gggtgctcat caccacccgc ggtgacaaag
      721 ggagtcccga ccctgacgtc gattccgtcg cgctcacccg cctccgggtc gaggagaccg
      781 ccaaggctgg tgcactgctc ggcttcgccg atcacttcca cctcgactac ggagacggcg
      841 agcttcccga cgacctgaat ctgcgagccg cgatcgtccg acacgtccgg gacctgcgcc
      901 ccgaagtcgt cctctgcccc gaccccacgg cggtgttctt cggcgacgcg tacttcaacc
      961 atcgcgacca tcgggcaaca ggctgggcga cgctcgacgc cgtcgccccc gcggcgggca
     1021 acccccatta cttccgggac cagatcgtcg ccggcgtccc gacccaccac gtgcgggccg
     1081 tctacctctc gggcacattg gagccgaact gctgggtcga tatcagcgac gtgctcgaac
     1141 gcaagatcga cgcgttgttc tgtcacgcca gccagctcac cgagaccggg gagtggttcc
     1201 gcgagttcct gcgcgaacgc gccgaggacg cgggacgcac cgccggtgtt cgctacgccg
     1261 aggcgttccg gcgcctcacg ttcggttcct gatcagccgc gtggtcggcg cttccactcc
     1321 gccatggccc gcaacaccag taccgccacg atcccgaccc cgatcgcacc ggtcagcgcg
     1381 ccggcgagcg tcccgacgac cttcgaggtg ctgcagttgc cggtgcagcc cacgtcgacg
     1441 agcccgtatc cgatgatccc gccgaacaca cccgcgagca cgacgctccc gaacgcgagc
     1501 agataggcgc cacgcgaggg aaggccggcg ggcacaccac ccgtgtcgag ctcgtcgtca
     1561 tcgccagcca cggcccggat cgtatggcca ggggccgccg gtaggctcgt cggcgatgcg
     1621 agagtggctc gtcgccggcg cgctcgtcga gatcgacggc gcgctcctcc tcgtgcgcaa
     1681 cgaacgccgg ggcggccact ccgactggac gacacccggc ggcgtcatcg acgccgacga
     1741 ccagtcactc ctcgacggcc tcgcccgcga ggtctacgag gagaccgggc tgcgcgtcac
     1801 cgcctgggaa ggtccgctgt acgaggtgaa cgccgtcgcc cgcgacatgg actggcgtct
     1861 ccgcgtggag gtgcaccggg cgatcgagtt cgagggtgag ctgcgcgtcg ccgaccccga
     1921 cggcatcgtc gtcgaggcgg agttcatcgc gacgccgggc tgcctggccc gcctcgccgg
     1981 cggcgcaccg tgggtgcgtg agccgctcgt cgactggctc gccgaacgct ggggcccgga
     2041 cgcggcccgt ggctactcct acgaggtgcg cgggagccga cgcgaggacc tgtcggtgga
     2101 gcgcaccctg ccgatgaccg ggtgagcgtg gtgtccgagc ctggcatcct ccacgtcgac
     2161 ctcgacgcgt tctacgcctc ggtggagcaa ggcgccgacc ccagcctcca gggccgaccg
     2221 gtagtggtcg gcggactcgg cccgcgtggt gtcgtcgccg cctccagcta cgaggcccgc
     2281 cagttcggga tccagtcggc catgccgatg gcccgcgccc ggcgagcctg ccccgacgcg
     2341 gtgtttctcg caccccgctt cgacgcgtac gccgccgcta gccgcgccgt catggcgatt
     2401 ctgcggtcct acacgccgct cgtcgagccg atctcgctcg atgaggcgtt cctcgacgcc
     2461 cgcggcgccc gccgactcca cggccctgcc cgaacgatcg cgctcgagat ccgcgcccgg
     2521 gtccgtgccg agaccggcct caccgcctcc gtcgggctcg ccacgacgaa gctcctcgcc
     2581 aagatcgcca gcgacctcgg caagcccgac gggttcctca tcgtcgaacc cgggaccgag
     2641 ctcgacttcc tccatcccct cgacgtcggg cggctctggg gggtggggcc ggccacccgt
     2701 cgccgactcg aacggctcgg cgcgcacacc atcggcgacc tcgccgtgat ccctgaatcc
     2761 accctgtgcc acaccctcgg cgacgcgcac gggtcgcacc tccactccct cgcgcacaac
     2821 gacgactcgc gcccggtgga gcccgaacgg cacgccaagt cgatcgggca cgaggagacg
     2881 ttctcgaccg acgtcaccga ccggcaccgc ctcgaagtcg acctgatgcg attcgccgat
     2941 cgggttgcca cccgcctccg cgaaggagcc gtcgccgccc gcaccgtgca gctcaaggcc
     3001 cggtacgccg actttcggac gatcacccga tcccgaacgt tgcccgacgc caccgacgtc
     3061 gcccaggacc tcgtcaccgt cggacgcgag ctgctgcgag agatcgacct cgaagacggt
     3121 ctgcgcctgc tcggtctctc ggctcagcag ctcgtgccga tcaccgaggt ccagccccgc
     3181 ctcccactcg gcgtccgtac cgacgtgtct cgtcacgcct cactcgagcg gaccatcgac
     3241 gaggtccggg ccaggtacgg cgacgacgcc gtgacccgcg ccccgcgagc accggccggc
     3301 cccgaacgga gccggccgtg accgttcgtg tcgggatcgt cggctgcggc cacatcggct
     3361 tcatccactc gtacacgctc caccagctca tgaagcacgg cctggtcgat gcctgcgtca
     3421 ccgccaccta cgacgtcgtc cccgaacgcg ccgccacctt cgcgctcgcc cacggcgccg
     3481 tcgcctgcga caacgtcgat gcggtgctcg accaggtcga cgcggtgtgg gtctgcacct
     3541 ggaccgccgc ccaccacgat gtcgtggctg ccgccgcgac ccgcgggctg gccatcttct
     3601 gcgagaagcc cctcgcgccc acgttccccg agtgtgaagc cgtcgcccag atcttggcca
     3661 ccgtcccgca ccaggtcgga ctggtccttc gccacgcgcc ggtcttccag gccctcgtcg
     3721 acgaggtgac cacgggccgg cacggacgcc cgctcgccgt cgtcttccgc gacgaccagt
     3781 atttcccggt ccaaggggtc tacgcgtccg agtggcgagc cgacgtcgcg cgtgccggcg
     3841 gcggggcgtt gttcgagcac tccatccacg atgtcgacgt gctccgctgg ctgctcggag
     3901 atcccacgac cgtgcgggcc gatgtcgcgt cccggtacca gcatcccggg atcgacgatg
     3961 tcgcggcgct gaccctcacc ttcgccgacg acaccatcgc gacgctcacc agcgtctggc
     4021 accagatcct gagccgtggc tcgacgcgac gagtcgaggt gttctgcgac gacgcgctcg
     4081 tgtggctcga cgacgatcac ctgggacccc tccatgtcga gagctcggat cggtccgaga
     4141 tcatcgagcc cgaggcgcca ccttgggtcg gcgagctggc cgtcccgccg gccctgaccc
     4201 gcatggtggg ggagtacgcg ccggcgacga aggggttcct cgacgcgctc gccgctcacg
     4261 gcaccgccgc gaccgggttt cccgacgcgg cgaccgcgct ggccgcgcac cgggtcgtgg
     4321 acgccgccta ccggtccgcc gcggccggtg gggaagcaat cacgccgcca ccgcgttgag
     4381 gggggcgggg ggctgcgcga caatgaggcg cccgcccaac tgacggcccc ggggcccgag
     4441 ccggaccccg cacagaggag ctccgcccag cgccatgccg ctttccgaag acgagcaccg
     4501 gatcctcaag gagatcgagg ccaacctctc ggtcaccgac ccgaagctgg tgcagcaggt
     4561 ttccgacacc accctctacc ggcacgcagc cgggatgatc aaatgggctg tcgccgggtt
     4621 cgtcgtcggc ctcatcctct tgatcgcgac gttcaccagc gtcctcgcgc tcggtgtcgt
     4681 cggctttttg atcatgctgt tctgcctgct cgtcatcgaa cgcaacgtcc gcaagatggg
     4741 tcgggcgggc ctcgagagcc tgaccggatc tcttcgcggc ggcgcgctca agggcgcctt
     4801 cggcgatgcc ggccgccggt ggcgcgaacg ctggcaccgc gacgacgacc cgtcctgaac
     4861 ctctgagcgc ccggacgggc ttcagcgccc cccaggcggc gcgcctctct ggctgctcac
     4921 gctcccagtt ggagtactgg cagcgctgcg ggctcatcgt cgctcatccc gacgcgtccg
     4981 acggcccgta ctcctttcgc gacctggtgg cgttacgcat ggttcgctcc cttctcgacg
     5041 ccggcctgcc tctcgcccgg atccgacggg cggtggcggc ggtcatcgac gcgggcgagg
     5101 acatggccgg cctccggatg gtgaccgacg gggaccacgt ctgggcttgc cgcgacgacg
     5161 gccagatcct cgacgccctc cgccacggcc aactcgccct gttcgtcgcg gttgaccaga
     5221 tggccgccga cgtcgacact gaggtccggg cgttctccca ggaccgtcag accttcgtgg
     5281 accgcctccg cggcagcgac caccccgcgg ggtccggctg atccaaccgg cgcgtcgaga
     5341 actgggcccg caatcgggcc cgcagat
//
LOCUS       DATCCH010000037         2722 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_209557, whole genome shotgun sequence.
ACCESSION   DATCCH010000037 DATCCH010000000
VERSION     DATCCH010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209557"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VIJ44_00805"
     CDS             <1..214
                     /locus_tag="VIJ44_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164466.1"
                     /translation="DGAGLVQWLAIGLSFVAVIVILRYARQPRCEVCSRRHARPDLTT
                     QHPEARLLAVELPEAADSGAISWKAE"
     gene            296..1057
                     /locus_tag="VIJ44_00810"
     CDS             296..1057
                     /locus_tag="VIJ44_00810"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5164467.1"
                     /translation="MPGSIAVVTGASSGIGHRLALELAATGATVVALARREERLAALT
                     PQLRQSSPESHHITCDVRDTTRFVATLRDVEQRHGRIDILINNAAIPEPEGDDLDRYR
                     SVMETNYFAVVAGTLAVLPGMRSRRRGVIVNVSSDTARAPTPGEAGYAASKAAVSAFT
                     EALSFDVEDDGVFLHILYPGWVPTEMTQSDTGDQHLPPRFVRRTPEQVARLVVAGLGK
                     RRVELDATKLARIAPVARTFFPSAYRRGIRASLRT"
     gene            1324..1905
                     /locus_tag="VIJ44_00815"
     CDS             1324..1905
                     /locus_tag="VIJ44_00815"
                     /inference="COORDINATES: protein motif:HMM:TIGR03086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03086 family metal-binding protein"
                     /protein_id="HEY5164468.1"
                     /translation="MDALIAYEQTLVWTGQRVAGVRADNLGVATPCRDWNARALVTHV
                     VAGIWYFKALASNERVEELMSGLSDLVGDDPFAAYDRAARAGLEAWRTPGVLDRSYAM
                     PLGDQAGREALAIHQADLLIHGWDVAEATHQDATMSPELAEFALRTERSFIRPEMRGA
                     GRAYDAARSASGSAGPQDHLLAFVGRSPTWRAA"
     gene            complement(1922..>2722)
                     /locus_tag="VIJ44_00820"
     CDS             complement(1922..>2722)
                     /locus_tag="VIJ44_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164469.1"
                     /translation="VPNCGNIDPLGVTSNVVADQTTGTVYAVGERETGVPSPHPAEFV
                     MVGVNPATGVLSLQSPSLTAPLGTDVPRQQQRAGLLLANGNVYVGFGGLAGDCGSYHG
                     YVVAARESDGVVMGSFKVAPANNAGAVWGTSGPVADSLGNVYATTGNSFNAPAGTDWS
                     DAVLKLNSSAGLLDYFQPVQWRSDNNADLDLGSTGPVLLPSGTQVFAIGKQHDAFLLN
                     VSSLGGVDHSTPVGTLNACPGMAFGQNAVIGASAYVACSSGLRQVHLS"
BASE COUNT          426 a          953 c          920 g          423 t
ORIGIN      
        1 ggatggggca ggactggtgc agtggctggc gatcgggctc tcgttcgtcg cggtcatcgt
       61 gatcctgcgc tacgcccgcc aacctcgctg cgaggtgtgt tcccggcgac acgcccgccc
      121 cgacctcacg acccaacacc ccgaggcccg gctcctcgcc gtcgagctgc ccgaggccgc
      181 cgactccggc gcgatcagct ggaaggccga gtagcccgcg ctccggcgcc gacgtcgtcc
      241 cgctgtggga cgaagatggg gcgggttctg acccgaggga gtgacgatgt tcaacgtgcc
      301 gggatcgatc gcggtggtga cgggcgcgtc gtcggggatc ggccaccgtc tggcgctcga
      361 gctggccgcg accggcgcca ccgtcgtcgc gctcgctcga cgggaagagc gactcgcggc
      421 actgacaccg cagctgcgac agtcgtcccc cgagtcgcat cacatcacct gcgacgtcag
      481 ggacaccacc cgctttgtcg cgaccttgcg cgacgtcgaa cagcgacacg gccgcatcga
      541 catcctgatc aacaacgcag cgatccccga acccgaaggt gatgacctcg accggtaccg
      601 cagcgtcatg gagaccaact acttcgcggt cgtcgcgggc acgctggcgg tcctcccggg
      661 gatgcgctcc cgacgacgag gtgtgatcgt caacgtctcg tcggacacgg cccgcgctcc
      721 gacaccgggt gaggccgggt acgcggcgtc gaaagcggcg gtcagcgcgt tcaccgaagc
      781 gctgtcgttt gacgtcgagg acgacggtgt cttcctccac atcctgtacc cgggttgggt
      841 gccaaccgag atgacccaat cggacaccgg cgaccagcac ctcccacccc gctttgttcg
      901 ccggaccccg gagcaggtcg cccgactcgt cgtcgccgga ctcgggaagc gtcgggtcga
      961 gctcgacgcc accaagctcg cccgcatcgc gccggtggcg cgcaccttct tcccgtcggc
     1021 atatcggaga ggcattcggg cgtcgctgcg cacctagtcc gccgacaccc cgtcgtccgt
     1081 gaccgccggg ccgaggaagt cagtcgttct cggtcggcac gaacccgagc cgcgctcgac
     1141 tttggtcgcg ctccgaggcc tggaggcgtt cccccaaacc gagcccagag cacggaggcg
     1201 cccggaggag gggagcccag accgggttcg cttggggctc tacattcgag ccatgggcag
     1261 gtccacgatg agcgcttctc gtcaggaccg gcccgcaggt gggtgtcgcc gggacggcgt
     1321 gtgatggatg ccctcatcgc gtacgaacag acgctggtgt ggaccgggca acgcgtcgcg
     1381 ggtgtccgag ccgacaatct cggtgtcgcc acaccgtgtc gcgactggaa tgctcgcgcg
     1441 ctcgtgacgc acgtcgtcgc cgggatctgg tacttcaagg ctctggcctc gaacgagcgc
     1501 gtcgaggagt tgatgagcgg cctctccgat ctcgtggggg acgacccgtt cgcggcctac
     1561 gaccgcgcgg cgcgcgccgg actcgaggcc tggcgcaccc ccggcgtcct cgaccggagc
     1621 tacgcgatgc cgctcggcga ccaggcaggc cgcgaggcac tcgcgatcca ccaagccgat
     1681 ctgcttatcc acggctggga cgtcgccgag gccacccatc aggacgccac tatgtcgccc
     1741 gagctcgcag agttcgcgct gagaaccgaa cggtccttca tccgacccga gatgcgaggc
     1801 gccggccgcg cctacgacgc ggcgcgaagc gcttccggtt ccgcggggcc ccaagatcac
     1861 ctgttggcat tcgtcgggcg ctccccgacg tggcgggcag cctgagcacc actgccgcgg
     1921 gttagctgag atgcacctgt cgcagcccgg atgagcaggc gacataggcc gacgccccga
     1981 tcaccgcatt ctggccgaag gccatcccgg ggcacgcgtt gagcgtcccg accggagtgc
     2041 tgtgatcgac tccgccgagc gaactgacgt tgagtaggaa cgcgtcgtgc tgcttgccga
     2101 tcgcgaacac ctgcgtcccc gacgggagca ggaccggtcc ggtcgacccg aggtccaggt
     2161 cggcgttgtt gtcggaccgc cactggacgg gctggaagta gtccagcagt ccggcgctgc
     2221 tgttcaactt caggaccgca tcgctccagt ccgtgcccgc gggtgcgttg aagctattgc
     2281 cggtcgtcgc gtacacgttg cccaagctgt cggcgaccgg accgctagtc ccccacacgg
     2341 ctccggcatt gttcgccgga gcgaccttga agcttcccat tacgacgccg tcgctctctc
     2401 tcgccgcgac gacgtagccg tggtagctgc cgcagtctcc ggcgagcccg ccgaacccga
     2461 catagacgtt gccgttcgcg agtaggagac cggcccgctg ctgttggcga gggacgtcgg
     2521 tacccaacgg ggcggtgagg ctcggcgact gaagggacaa gacaccggtg gccggattga
     2581 cgccgaccat cacaaactcg gcgggatgcg gggaggggac gcccgtctcg cgctcgccga
     2641 cggcgtagac cgtgccggtg gtctggtcgg cgaccacgtt gctggtcaca ccaagcgggt
     2701 cgatgttccc gcaattcggg ac
//
LOCUS       DATCCH010000038         3885 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_253262, whole genome shotgun sequence.
ACCESSION   DATCCH010000038 DATCCH010000000
VERSION     DATCCH010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3885
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253262"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1011)
                     /gene="glp"
                     /locus_tag="VIJ44_00825"
     CDS             complement(<1..1011)
                     /gene="glp"
                     /locus_tag="VIJ44_00825"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEY5164470.1"
                     /translation="MPLTPLEAVQQAILASMRPLDVRDVDLGDALGLVLAVDVTTDQP
                     VPPFANTAMDGYAIQAADTVTATVDSPVRLRVVGDLPAGKAPGRAVNPGEAIRIMTGA
                     PMPDGADSVVMVERTRRDGDAAVLVEEAAQSGDHVRGAGGDLAAGAPVFAAGTVVGPA
                     HVGVLASIGVARVPVIPRARVGVLSTGDELTAVDAPGPLAPGHIRDSNRPMLLALARQ
                     AGCDAVDLGIAVDDEAGIVATLDDAVRRYDAVLTSGGVSVGDYDYVKAALERLGSLTS
                     WQVAIKPSKPLAFGVVGDVPVFGLPGNPVSSLVSFECFARPALLERMGHRHRFRPEVT
                     ARA"
     gene            complement(1011..1901)
                     /gene="galU"
                     /locus_tag="VIJ44_00830"
     CDS             complement(1011..1901)
                     /gene="galU"
                     /locus_tag="VIJ44_00830"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019873309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEY5164471.1"
                     /translation="MTKRPSPITKAVIPAAGMGTRFLPATKSQPKEMLPIVDKPSIQY
                     VVEEAVRAGLRDVLVITGRGKRAIEDHFDRNFELEFYLEQDRKDELLREVQATNEIAD
                     LYYIRQRDPLGLGHAVSVARDHIAGESFAVMLGDDIMVDDARLLRTMLEVHAAEGGSV
                     LALMEVAPDEISAYGCAEVDAVRDRLVRVRSLVEKPPPDQAPSNLAVVGRYVFSPQIF
                     PALDATEPGVNGELQLTDAIAQLLDEQPIHGVVFSHGRYDIGRKIDFLRANIELALDR
                     PDLGPELRAFLVDLVQRRQL"
     gene            complement(1898..2506)
                     /locus_tag="VIJ44_00835"
     CDS             complement(1898..2506)
                     /locus_tag="VIJ44_00835"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HEY5164472.1"
                     /translation="MILVACLVPVAYLVGSFPSAGLIARTRGRDVLREGSGNPGASNV
                     FRLLGWKAGLAVLLLDLGKGAGAAGVGVAIDGHRGAFILGAAAVVGHVFPVTRRFKGG
                     RGVATAAGVMAVIFPLEIAVLAVVWFGIARGLRKASVASIVCAIAFPVIVAARDGSAL
                     DLAMTAGLAALVVARHLANLRRLVRGQELGLDDEDDGGSVAA"
     gene            2654..3076
                     /locus_tag="VIJ44_00840"
     CDS             2654..3076
                     /locus_tag="VIJ44_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HEY5164473.1"
                     /translation="MPTYRYRCSGCGEEFELWQSIKDDSLRTHEDGCGGEVLKVLTAA
                     GIVLKGSGFYKNDSRSSDGKRKGSDGSTPKQDSNDSKEGKDSKEGTDSKDSQDSGGSS
                     ESKPSSSESGTDSKRGESTSSTTKPGDSKSTAGAAKPS"
     gene            3124..3774
                     /locus_tag="VIJ44_00845"
     CDS             3124..3774
                     /locus_tag="VIJ44_00845"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEY5164474.1"
                     /translation="MIFDYGGVISARFLRDLHLFEDDMGYPRGSVEELMFGPSADTDG
                     EAVHDFHRLETGHLSLADYLQGLERRAPEVLGHPLDLEAYRAFAGASAVTVHWPVVHC
                     IRRLQATPLRLALLTNNVREFGDAWRATFPVEELFGTVVDSSAVGMRKPDPAIYAYTC
                     NLLEVTPTAAVFLDDNDDNIAAAAAMGLETVHVGVDPIVAIDELDTILERRGISSR"
BASE COUNT          626 a         1329 c         1399 g          531 t
ORIGIN      
        1 ggcgcgcgcc gtcacctcag gacggaaccg atggcggtga cccatccgct ccaggagcgc
       61 cggacgggcg aagcattcga agctgaccag ggacgacacc gggttgccgg gcagcccgaa
      121 caccggcacg tcgccgacga caccgaaggc gagcggtttg gagggcttga tcgcgacttg
      181 ccacgaggtg agcgacccga gtcgttcgag tgccgccttc acgtagtcgt agtcaccgac
      241 ggacacgccg ccgctggtca gcacggcgtc gtaccgacgg acggcgtcgt cgagggtggc
      301 gacgatcccc gcctcgtcgt cgacggcgat gccgaggtcg acggcgtcgc agccggcctg
      361 gcgggccagc gccaggagca tcggacggtt cgaatcacgg atgtgtccgg gggcgagcgg
      421 cccgggcgcg tcgacggcgg tcagctcgtc accggtggag agcaccccga cccgggcgcg
      481 tgggatgacc ggcacccgag cgacgccgat gctggcgagg actccgacgt gcgccggtcc
      541 caccacggtg ccggcggcga agaccggcgc gccggccgcg aggtccccgc cggcgccgcg
      601 cacgtggtcg ccgctctggg cggcctcctc gacgagcacc gcggcgtcgc cgtcgcgtcg
      661 ggtgcgttcc accatgacga ccgaatcggc gccgtcgggc atcggggcgc cggtcatgat
      721 gcggatcgcc tcaccggggt tgaccgcccg gccgggggcc ttcccggcgg gcaagtcgcc
      781 gaccacgcgg agccgcacgg gagagtcgac ggtcgcggtg acggtgtcgg cggcttggat
      841 cgcgtagcca tccatcgcgg tgttcgcgaa cggcgggacc ggctggtcgg tggtcacgtc
      901 gacggcgagg accagcccca gcgcgtcacc gaggtcgacg tcgcgcacgt cgaggggacg
      961 catggaggcg aggatcgcct gctggacggc ctccaggggc gtcaggggca tcagagctgg
     1021 cggcgctgca cgaggtcgac gaggaaggcc cgcagctcgg gtccgaggtc gggacggtcg
     1081 agggcgagct cgatgttggc ccgcaagaag tcgatcttgc ggccgatgtc gtaccggccg
     1141 tgggagaaca ccaccccgtg gatcggctgt tcgtcgagca gctgggcgat ggcgtcggtc
     1201 agctgcagct caccgttcac tccgggttcg gtggcgtcga gcgccgggaa gatctgcggt
     1261 gagaagacgt accgtccgac caccgccaga ttcgacgggg cctggtccgg cggtggcttc
     1321 tcgaccaggg agcggacccg caccaggcgg tcacggaccg cgtcgacctc ggcgcacccg
     1381 tacgccgaga tctcgtccgg cgcgacctcc atcaacgcga ggaccgagcc gccctccgcg
     1441 gcgtgcacct cgagcatggt gcgcagcagg cgagcgtcgt cgaccatgat gtcgtcaccg
     1501 agcatcaccg cgaacgactc accggcaatg tggtcccggg ccaccgacac cgcgtggccg
     1561 agccccagcg ggtcgcgttg gcggatgtag tagagatcgg cgatctcgtt cgtcgcctgc
     1621 acctctcgga gcagctcgtc cttgcggtcc tgctccagat agaactcgag ctcgaagttg
     1681 cgatcgaagt ggtcttcgat ggcgcgcttg cctcgccccg tgatcacgag gacgtcgcgc
     1741 agcccggccc gcacggcctc ctccacgacg tactggatgg acggcttgtc gacgatgggc
     1801 agcatctctt tgggctggct cttcgtcgcg ggcaggaacc gggtgcccat gcccgccgcg
     1861 gggatgacgg ccttggtgat cggcgaggga cgcttcgtca cgcggcgacg ctaccgccat
     1921 cgtcttcgtc gtcgagcccg agctcttggc cgcgcaccag gcgccgcagg ttggcgaggt
     1981 ggcgcgccac gaccaacgcg gccagcccgg cggtcatcgc gaggtccagc gcggagccgt
     2041 cgcgggccgc gacgatgacc gggaacgcga tggcacacac gatcgacgcc accgaggctt
     2101 tgcgcagccc ccgggcgatc ccgaaccaga ccacagcgag gaccgcgatc tcgagcggga
     2161 agatcacggc catgacaccg gcggcggtcg cgacgccacg accacccttg aagcgccgcg
     2221 tcacggggaa gacgtgtccg acgactgcgg ccgctccgag gatgaacgcc ccgcggtggc
     2281 cgtcgatcgc aacgccgacc ccggccgccc cggcgccctt gccgaggtcg aggagcaaca
     2341 ccgccagtcc ggctttccat ccgagcagac ggaagacgtt ggacgccccg gggttccccg
     2401 acccctcgcg gaggacgtca cgccctcggg tgcgcgcgat gagcccggcg ctggggaagc
     2461 ttccgaccag gtacgccacc gggacgaggc atgcgacgag gatcaccgcg ggcattcttc
     2521 cgctctgggc ggccctctgg gatgacgagg gaccgtcccg gcgtttacca ttagcagtca
     2581 acggtcccga gtgccagccg ctttggcggt gccctgggcc ttggccgggc aaccggcccc
     2641 gagcggaggc gacatgccca cctatcgata tcgctgcagc gggtgtggcg aggagttcga
     2701 gctgtggcag tcgatcaagg acgactccct ccgaacccac gaggatggat gcggcgggga
     2761 ggtcctgaag gtcctgaccg cagccgggat cgtcctcaaa ggctcgggtt tctacaagaa
     2821 cgacagccgg tcctcggacg gcaagcgcaa gggctcggac gggtccacgc cgaagcagga
     2881 cagcaacgac agtaaggaag gtaaggacag taaggaaggt accgacagta aggacagtca
     2941 ggacagcggc ggctcctcgg aatcgaagcc ttcgtcgtcc gagtcgggaa cggactccaa
     3001 gcgcggcgaa tcgacgtcgt cgacgaccaa gccgggcgat tcgaaatcga ccgcgggcgc
     3061 cgcgaagccc tcgtgagcga ggggtcaccc gctgggggtc cggaccgggg tgccatcgcg
     3121 gcggtgatct tcgactacgg cggggtgatc tcggcgcggt tcctgcgaga cctgcacctc
     3181 ttcgaggacg acatgggcta cccgcgcgga tcggtcgagg agctgatgtt cgggccgtca
     3241 gccgacaccg acggtgaggc ggtccatgac ttccaccgcc tcgagaccgg gcacctgtcg
     3301 ctcgccgact acctgcaggg ccttgagcgg cgcgcccccg aggtgctcgg ccaccccctc
     3361 gatctcgagg cgtaccgggc gttcgcgggc gcgtccgcgg tcacggtgca ctggccggtc
     3421 gtgcactgca tccgacgtct gcaggcgaca ccgctgcgac tcgcgctgct gacgaacaac
     3481 gtccgcgagt tcggcgacgc ctggcgggcc accttccccg tcgaggagct cttcggcacg
     3541 gtcgtggact ccagcgcggt cgggatgcgc aagcccgacc cggcgatcta cgcgtacacg
     3601 tgcaacctgt tggaggtgac gccgaccgcg gcggtgttcc tcgacgacaa cgacgacaac
     3661 atcgccgccg cggccgcgat gggactcgag acggtgcacg tcggcgtcga cccgatcgtg
     3721 gccatcgacg agctggacac gatcctggag cggcgcggca tcagctcccg ctgacctgca
     3781 tctcgccgat gagcacggtg gccccggcgg acgaaccggg cagccagcgc cgatcggcgc
     3841 ccacctcgac gacgtcgagg agcatgcgtt gcagcgtcga cgcca
//
LOCUS       DATCCH010000039         5660 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_295178, whole genome shotgun sequence.
ACCESSION   DATCCH010000039 DATCCH010000000
VERSION     DATCCH010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5660
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295178"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VIJ44_00850"
     CDS             <1..346
                     /locus_tag="VIJ44_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013731757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY5164475.1"
                     /translation="QHPPPGPARRNNPLCLPENLERATPIIETIRRVASAHDATPAQV
                     ALAWLLTKPNVVAIPGASSIEQLERNVAAADLDLTEDEVVELTAESDRFQPISGPATG
                     LALLRARRSTAA"
     gene            complement(646..1365)
                     /locus_tag="VIJ44_00855"
     CDS             complement(646..1365)
                     /locus_tag="VIJ44_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164476.1"
                     /translation="MGTFPAGFIIVKREASVNDSTWERYGLAAGIAFVVLVVVAALIG
                     GAPPKPTDSTAKILSYFRDNQDALKVGSYLNGLATVTFIWFLGSLWSRLRRAEGTGSR
                     LSVIALVGGIASVAFAIVASGIVAFVALYVGELGGGGAQAFYLLATVFLAFSAFALAV
                     FTSATSIMILRYPIVEKFLGWVGEGIATLWLVAGIAVADNNAAIHTVGFIAFLVWAGW
                     LLILSVLLLTRDVAAVETTAH"
     gene            1576..1896
                     /locus_tag="VIJ44_00860"
     CDS             1576..1896
                     /locus_tag="VIJ44_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164477.1"
                     /translation="MGERGLRDTIGVAVATVTAGAGLIITLAAFDIASGTQALHPRHT
                     PSGQDIDMKPSDFQNLHTMTMVGGFFVDNRLGHLKAALRVARAGKGVYPVGTILQLGP
                     KKRW"
     gene            1978..2184
                     /locus_tag="VIJ44_00865"
     CDS             1978..2184
                     /locus_tag="VIJ44_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164478.1"
                     /translation="MTRGTTTVVNRFGGNCASCHVAAQAKFDMVCEHNHGCAPLPVGD
                     DVIASIQRADPRPLPTPLTTPPTR"
     gene            2243..3616
                     /locus_tag="VIJ44_00870"
     CDS             2243..3616
                     /locus_tag="VIJ44_00870"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEY5164479.1"
                     /translation="MTGWRWAAIPMSGLVLGAGFASPAPTPLSGPHPACATTRAPPSA
                     PATAPAAAPRPSPLRLPALPLRTSGNRILDRRHREVTLASVNWYGAEEQDYIVGGLDR
                     RPLDVIARGVRALGFNSVRLPWSNALLHDNPVVCDSSVAANPTLRGKHALAVLDAVVR
                     ALGAQGIMVILDNHMTTADWCCLPTEANNLWYNAAPTSSQPLWLQNFHTAQWLADWQT
                     MVRHYTGPRYRNVVGVDLRNEPRGGAVWGTQTGPSPGCAPAAPDNPDWHDAAQRGAAC
                     VLAANPDLLVMVEGVAYSLNLTGAAAAPVVLPVSHDHQLVYSPHNYSQDQPADVNADP
                     VRLAAHLNQYWGFLASGAAPTPIWLGEFGTCHTAQLQCGDNHDHSHEWIVNLASYLST
                     NRFSWGYWPLNGTQSRGITRVDGDEESFGVLDTTWGCSAQPALTALLRHLPGATPGTD
                     LHCVPRP"
     gene            complement(3645..4220)
                     /locus_tag="VIJ44_00875"
     CDS             complement(3645..4220)
                     /locus_tag="VIJ44_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164480.1"
                     /translation="MAEKPTYLGLLNTIAVVETRAHAYLTAWADVTPDPDVRAVLRTV
                     AAREGEHGMSFAKRVNELGFDVRCNDDPSFETRTEIARSDRTDLEKIEALELLRFRSD
                     DEPDVFDTMFRDHSIDIQTGALLGRYIAEERDTLRLLQGCCAQLQARGSEATPSSSRL
                     GVLEDKIDGVSRAVEELRQIVCAQAMPTSSV"
     gene            complement(4329..4586)
                     /locus_tag="VIJ44_00880"
     CDS             complement(4329..4586)
                     /locus_tag="VIJ44_00880"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HEY5164481.1"
                     /translation="MPGVPHPQGRIVQLASFPSRFEADVVIAMLASNEIRAAANYGDG
                     DGWAPHLALYQGARVLVFDDDLDRARELLDAASPLEDPTDN"
     gene            4711..>5660
                     /locus_tag="VIJ44_00885"
     CDS             4711..>5660
                     /locus_tag="VIJ44_00885"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5164482.1"
                     /translation="MTYSSGRIVHDADAHIMEPPTWLRDHADPEVRDRIEPLTYPSGN
                     ELRQTGDPGEQLRDLAAAFERLRARHGTEEYRRVEAAEIMQRKNFAATGSFVRDDRPR
                     ALDLLGFSSQLVFNTFHNRRLRDWEHGDDLDLAYGAARAHNRGMVEFCSVDRRLLPTC
                     YVPLADFDRAAAAADESIAMGAAALLVASGCPRHHSPSHIGLDPVWARAQEAAIPVVF
                     HVGGTGDLIDASYFRNGLPVPPDFHGGEENFRSVDYMGIPGPPAQTLATMIFDGVLDR
                     FPMLRIGVIEQGAIWVPSWLRHLEAAFEAFARHEERLRALA"
BASE COUNT          919 a         2096 c         1828 g          817 t
ORIGIN      
        1 gcagcatccg ccgcccggac cggcgcgccg gaataacccg ctctgcctgc ccgagaacct
       61 cgaacgggcg accccgatca tcgaaaccat ccgtcgggtc gcgagcgccc atgacgccac
      121 cccggcccag gtggcgttgg cctggctgct caccaagccg aacgtcgtcg cgatccccgg
      181 cgccagctcg atcgagcaac tggagcgcaa cgtcgcggcc gccgacctcg acctcaccga
      241 ggacgaggtg gtggagctca ccgccgagag cgaccggttc cagccgatca gcggtcctgc
      301 caccggtctg gctctcctcc gcgctcgccg gtccactgcg gcgtagccgg gagcggcact
      361 ttcggctctc gacgaggaac gccggcttca ggtgacgtga cgccagaaca cacgggcttc
      421 gagcacgccg agcacgagcg tcacaaccgc gttcagctga ggaccacggt catcggaatg
      481 ccccgaccgg tcggtgaccc gtcggcacga gtcacggcgc cgtctcctcg gggacacggg
      541 tcccgttcgc cgtcaggtca acgcgttctc gtcgtcgccg catggcggca gcgtacgacg
      601 gtgccgcggc gtcggcattg cctgcgcctc tccggacggt cacagctagt gcgcggtggt
      661 ctccaccgcg gctacgtcgc gggtcaacaa gagcacgctg aggatcagga gccaaccagc
      721 ccagaccaag aacgcgatga agccgacggt gtggatcgcg gcgttgttgt cggcaaccgc
      781 gatcccggcg acgagccaca gggtggcgat gccttcgccc acccagccga ggaacttctc
      841 gacgatcggg tatcgcagaa tcatgatcga cgtcgcggac gtgaagacgg ccagcgcgaa
      901 cgcgctgaac gccaagaaca ccgtggccag caggtagaag gcctgcgcgc cgcccccgcc
      961 gagctccccg acgtagagcg cgacgaacgc gacgatcccg ctcgcgacga tggcgaacgc
     1021 cacgctggcg atcccgccga cgagggcgat caccgacagt cggctgccgg tgccctcggc
     1081 tcggcgcagt cgggaccaca ggctcccaag gaaccagata aacgtcacgg tggcgagccc
     1141 gttgagatac gaaccgacct tgagggcgtc ctgattgtcg cggaagtagc tgaggatctt
     1201 cgccgtcgaa tccgtcggct tcggtggagc acctccgatg agcgcggcga cgacgaccag
     1261 gaccacgaag gcgatcccgg cggccaggcc gtaacgctcc caggtggaat cgttcacgga
     1321 tgcctcccgt ttgacgatga tgaagcccgc ggggaaggtc cccaccggca ctaggggcta
     1381 ctggacgcga gcgcgtcctg caagtagccg gagtgagaac gactccgacc agcacgaatg
     1441 tcgccgtcga agccacgaat cctggacaac gaccgcctcc ccccgacacc caatccgttg
     1501 cgccgagacc gccgtatacc ctccagaggc gtcgccgttc gacgcgaccg cacgacgaat
     1561 tcgatcggag aggcgatggg cgagcgcggc ctccgcgaca ccatcggagt cgcggtcgcg
     1621 accgtcaccg ccggcgccgg cctcatcatc accctggccg ccttcgacat cgccagcgga
     1681 actcaggccc tccacccccg ccacacaccg agcgggcaag acattgacat gaagccgagt
     1741 gacttccaga acctccacac catgaccatg gtgggcgggt tcttcgttga caaccggctc
     1801 ggtcacctca aagccgcgct gcgcgtcgcg cgggccggga agggcgtgta cccggtcggc
     1861 accatcctcc agctcggccc caagaagcga tggtgaagcg acgcaaacgg gttcaacccc
     1921 gcgacacgcg actgggagtt cttcttcctc cagaccacac cccgaggcac cacgatcgtg
     1981 acccggggca ccacgaccgt cgtcaaccgg ttcggcggga actgcgccag ctgtcacgtc
     2041 gccgcccaag ccaagttcga catggtctgt gagcacaacc acggctgcgc gcccctccct
     2101 gtcggcgacg acgtgatcgc gtccatccaa cgcgccgacc cccggccact ccctaccccg
     2161 ctgaccaccc cgcccacccg ctgaggcggc ccggttccgc tcacggccct gcggacgggg
     2221 cgggcgttct accatccgcc cgatgaccgg ctggcggtgg gccgcgatcc cgatgagcgg
     2281 gctggtcctc ggcgccggat tcgcctcccc tgccccgacc ccgctgtcgg gtccgcatcc
     2341 agcctgcgcc accacgcgag ctcctccgtc cgcgccggcg accgcgccgg cggcggcacc
     2401 gcgcccctcg ccgctgcgtc tcccggcgct gcccctgcgg accagtggga accgcatcct
     2461 cgaccgccgc caccgggagg tgacgctcgc cagcgtcaac tggtacggcg ccgaagaaca
     2521 ggactacatc gtcggcggcc tcgaccggcg acccctcgac gtcatcgcac gcggggtccg
     2581 ggccctgggc ttcaactccg tgcgtctccc ctggtccaac gccctcctcc acgacaaccc
     2641 cgtggtctgt gactcgagcg tcgcggccaa cccgacgctg cgtgggaagc acgccctcgc
     2701 ggtactcgac gccgtcgtcc gcgcccttgg cgcgcagggg atcatggtga tcctcgacaa
     2761 ccacatgacg accgccgact ggtgctgtct gcccaccgag gccaacaacc tctggtacaa
     2821 cgcggcaccg acctcgtcac aacccctctg gctccagaac ttccacaccg cgcagtggct
     2881 ggctgactgg cagacaatgg tccgccacta caccggaccc cgataccgca acgtcgtcgg
     2941 cgtggacctc cgcaacgagc cgcgcggcgg cgccgtctgg ggaacccaaa ctgggccgtc
     3001 accgggatgc gcgcccgcgg cgccggacaa tcccgactgg cacgacgccg cgcaacgcgg
     3061 cgcggcgtgc gtcctggccg ccaaccccga cctgctcgtc atggtcgagg gcgtggcgta
     3121 ctcgctcaac ctcaccggcg ccgcagccgc gccggtggtc ctcccggtca gccacgacca
     3181 ccagctcgtc tactccccgc acaactacag ccaggatcag cccgccgacg tcaacgccga
     3241 tcccgtccgg ctggccgccc acctgaacca gtactggggg ttccttgcgt cgggcgccgc
     3301 gccgaccccg atctggctcg gcgagttcgg gacctgtcac accgcccagc tgcaatgcgg
     3361 cgacaaccac gatcactccc acgagtggat cgtcaacctc gcgagctacc tcagcaccaa
     3421 ccggttctcg tggggctact ggcccctgaa cggaacccag tcccggggca tcactcgcgt
     3481 cgatggcgac gaggaatcct tcggcgtgct cgacaccacc tggggctgtt cggcgcagcc
     3541 ggccctcacc gcgctcctga gacacctacc cggagcgacg cccggcaccg acctgcactg
     3601 cgtcccccga ccctgaccgt gtttgtgccc ccgtcgggcg cggctcagac gctgctggtc
     3661 ggcatcgcct gcgcgcagac gatctggcgg agctcctcga cggcgcggga cacgccgtcg
     3721 atcttgtcct ccaagacgcc gagccggctg ctcgaaggcg tcgcctcgga cccgcgggcc
     3781 tggagctgcg cgcagcaccc ttggaggagc cgcaaggtgt cgcgctcctc ggcgatgtag
     3841 cggccgagta gcgcaccggt ctgaatgtcg atcgagtggt cccggaacat ggtgtcgaac
     3901 acgtccggtt cgtcgtcgga gcggaaccgg agcagctcga gggcctcgat cttctcgaga
     3961 tccgtgcggt ccgaccgggc gatctccgtc cgggtctcga agctcgggtc gtcgttgcag
     4021 cgcacgtcga agccgagctc gttgacgcgc ttcgcgaagc tcatcccgtg ctctccctcg
     4081 cgggccgcca cggtgcgcag cacggcgcgc acatcgggat ctggggtcac gtcggcccag
     4141 gccgtcaggt acgcgtgggc gcgggtctca acgaccgcga tcgtgttaag cagcccgaga
     4201 tacgtcggct tctcggccat ggtggatcct ttcggtctcg gtccgtgcgc cgacagggga
     4261 gcgagcgtcg gcgagtcgat ccactgtgcc aggcgggccg tgagacccgc aacaactccg
     4321 ggctgggtct agttgtcggt cgggtcctcg aggggcgacg ccgcgtcgag aagctcgcgg
     4381 gcgcggtcga gatcgtcgtc gaacacgagc acccgcgctc cttggtagag agcgaggtgg
     4441 ggcgcccagc cgtcgccgtc gccgtagttc gcagcggcgc ggatctcgtt gctggccagc
     4501 atcgcgatga cgacgtcggc ttcgaaccgc gacgggaacg acgccagctg cacgattcgt
     4561 ccttgtggat gcgggacgcc gggcatccga ccacggtacc ggcgccggta acggaccttg
     4621 tatcgaccgt gacccgtgcg cccgggaaga cgcggcccgg atcacgtctc ggtacgctgc
     4681 acacggtcca ggtgtcacgg gggagagccc atgacgtaca gcagcggacg gatcgtccac
     4741 gacgcggacg cgcacatcat ggagcccccg acgtggctgc gtgaccacgc cgatcctgag
     4801 gtccgcgacc ggatcgagcc gctcacctac ccgagcggca acgagctgcg ccagaccggc
     4861 gaccccggcg agcagctccg cgacctcgcc gcggcgttcg agcggctccg cgcccgccac
     4921 ggcacggagg aataccgccg ggtcgaagcc gccgagatca tgcagcgcaa gaacttcgcg
     4981 gcgacgggat cgtttgtccg cgacgaccgg ccccgcgccc tcgacctgct cggcttctca
     5041 agccagctgg tgttcaacac cttccacaac cgtcggctcc gggactggga gcacggtgac
     5101 gacctcgacc tcgcgtacgg cgcggcgcgg gcccacaacc gcgggatggt cgagttctgc
     5161 tccgtcgatc gacggctgct tcctacgtgc tacgtgccgt tggccgactt cgatcgggcc
     5221 gcggcggccg ccgacgaatc gatcgcgatg ggcgcggcgg cgcttctggt ggcttcggga
     5281 tgtcctcggc accattcacc gagccacatc ggtctcgacc ccgtctgggc ccgggcccaa
     5341 gaggccgcga tcccggtcgt gttccacgtc ggggggaccg gcgacctcat cgacgccagc
     5401 tacttccgca acgggctgcc ggtgcccccg gacttccacg gtggcgaaga gaacttccgt
     5461 tccgtggact acatgggcat ccccggccct ccggcccaga cgcttgccac gatgatcttc
     5521 gacggggtcc tcgaccggtt cccgatgctg cggatcgggg tgatcgagca gggagcgatc
     5581 tgggtgccgt cgtggcttcg tcacttggag gctgcgttcg aggcgttcgc tcgccacgag
     5641 gagcgcctgc gggcgctggc
//
LOCUS       DATCCH010000040         3978 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_502722, whole genome shotgun sequence.
ACCESSION   DATCCH010000040 DATCCH010000000
VERSION     DATCCH010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502722"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="VIJ44_00890"
     CDS             <1..453
                     /locus_tag="VIJ44_00890"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5164483.1"
                     /translation="ARVIDRLAPEADSQELALDLSLDPAPVRGASALLERLVENLVQN
                     ASQYNHRAGWISVVTKTEASQAVLRVANSGPLVPPEVVSSLFDRFRRLDGTGDHRHRG
                     FGLGLSIVEAVARAHDGTATAEAQPDGGLLVTVRLPAATVPAPVLTPS"
     gene            complement(495..1697)
                     /locus_tag="VIJ44_00895"
     CDS             complement(495..1697)
                     /locus_tag="VIJ44_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013424927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEY5164484.1"
                     /translation="MADNNVWILGAAMTKFGRYTDKDVIDLASEASMNAMNDGGVTIH
                     DMEVMGAGNLIEAASGIGQRIQKQIGQTGIPVYNVANACATGATAIRCVTMAIKAGEA
                     DMGLAVGCEQMGKAGLLGSSARADKQVYEPAGRYGAVMSVEGRLGTTLMPGVFAQAGM
                     EYAGEHDGVGFEQFAKVAEKNHAHSTLNPLAQYQKAFSLDQIMGADMMAYPNTLLMCC
                     PTGDGAAALVLVSEERLKTLSAEQRKRAVKLSASVMTSDPWTETDQVQPDVNTLTRAA
                     ADQAYEESGVDPKDLDLVELHDCFATAELIHYDNLRLCGPGEAGKFIDERGPWRDGST
                     PVNVSGGLISKGHPLGATGVANLYEVTTHLRGEAGDRQVEGAKVGLTHVIGLGSACAV
                     HVLEKAAV"
     gene            complement(1714..2124)
                     /locus_tag="VIJ44_00900"
     CDS             complement(1714..2124)
                     /locus_tag="VIJ44_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEY5164485.1"
                     /translation="MSETSPARVPLVEYLVIDDGEPHLIAHESVESGALYFDRRNADA
                     KSGKPGFTTRRLADTGRVRTFTIVHRASPGVPTPYVSAVVDLDGGGSVKANLLNVEPD
                     PAHVPFGLPVRLTTYVAGTDDNGTEAVAFAYEPA"
     gene            2172..2254
                     /locus_tag="VIJ44_00905"
     tRNA            2172..2254
                     /locus_tag="VIJ44_00905"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2205..2207,aa:Leu,seq:tag)
     gene            complement(2310..2633)
                     /locus_tag="VIJ44_00910"
     CDS             complement(2310..2633)
                     /locus_tag="VIJ44_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164486.1"
                     /translation="MPMRSDCRHYETRTYGSGEVVHRCKLNLAPEAPWRCPEECPSFT
                     KRRMDAGWHYGSLSTAGRGSETEPAGDDVAALLDAAQEIVNSAAPDILADFDGKKRRR
                     RRKRR"
     gene            2893..>3978
                     /locus_tag="VIJ44_00915"
     CDS             2893..>3978
                     /locus_tag="VIJ44_00915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY5164487.1"
                     /translation="MTPSGQARPADGPSTNRPDLPAGLVGLVGRTPDMVIAIDARGTI
                     VSANAATEKVLGHAASTKVGTAVLDLVHPDDRARAEAGLKALTTGGDAIAPVLVRARC
                     ADGSWLPIEVTATNLLEEPTVQAIVLIARDLSERRRLERRVHELEHGFTAAFRHSPVG
                     RTLVDLRGRWLLVNDAFARMVGRDANSLIGTRGSDVIHPDDRARVRAELASLAARAVD
                     STTVETRFIRSDGDTVWARFTVWLVFDDDGEPTHLGSDVTDVTELRAARDVEDRVRRG
                     FEALVEHSSDIITVLEPDGTWRSSSAAGSRLLGYERGFDPDGGILALVHPDDFEMTGR
                     ALDEVRRGTRGPNESLVVRIRAADGSYH"
BASE COUNT          539 a         1377 c         1411 g          651 t
ORIGIN      
        1 gcccgagtca tcgaccgcct cgcgccggag gccgacagcc aggagctggc cctcgacctc
       61 agcttggatc cagccccggt gcgcggcgcg tctgcgttgc tggagcgctt ggtcgagaat
      121 ctggtccaga acgcgtccca gtacaaccat cgcgcgggat ggatctcggt cgtcaccaag
      181 accgaggcca gccaggcggt ccttcgggtc gcgaacagcg ggcccctcgt gccgccggag
      241 gtcgtgtcga gcctctttga ccggttccgc cgcctcgacg ggaccggcga ccatcgccac
      301 cgtggctttg ggctggggtt gtcgatcgtc gaagcggtgg cccgggcaca cgacgggacc
      361 gcgaccgcgg aagcccaacc cgacgggggc ctgctcgtga ccgtgcggct gcccgcggcg
      421 acggttccgg ccccggtcct gaccccctcg taacgtcagg cccgccgacc cgggcacacg
      481 acggtgaggc gtgcttagac ggcggccttc tccagcacgt gcaccgcgca ggcggagccg
      541 agcccgatga catgggtcag gccgaccttg gcgccctcga cttggcggtc gccggcctcg
      601 ccgcgcagat gtgtggtcac ctcatagagg ttcgcgacgc cggtcgcgcc gagcgggtgt
      661 cccttcgaga tgagcccgcc ggagacgttg accggtgtcg agccgtcacg ccacgggcct
      721 cgttcgtcga tgaacttgcc ggcttcgccg gggccgcaga gccgaaggtt gtcgtagtgg
      781 atgagctccg cggtggcgaa gcagtcgtgc agctcgacga ggtcgaggtc cttcgggtcg
      841 acgcccgact cctcgtaggc ctggtcggcg gcggcacggg tcagcgtgtt gacgtcaggt
      901 tgcacctggt cggtctcggt ccaggggtct gaggtcatga ccgacgccga gagcttcacg
      961 gcgcgtttcc gctgctccgc ggagagggtc ttgagccgtt cctccgagac gaggaccagg
     1021 gcggcggcac cgtcgccggt ggggcagcac atcagcagcg tgttcgggta cgccatcatg
     1081 tcggcgccca tgatctggtc gaggctgaag gccttctggt attgcgccaa cgggttgagc
     1141 gtcgagtgcg cgtggttctt ctccgcgact ttcgcgaact gctcgaaccc gacgccgtcg
     1201 tgctcgccgg cgtactccat gccggcttgg gcgaacacgc ccggcatcaa ggtcgtgccg
     1261 agacggcctt cgacgctcat gaccgcgccg tatcggcccg ccggttcgta gacctgcttg
     1321 tcggcccgcg ccgacgagcc cagcagtccc gccttgccca tctgctcaca gccgaccgcg
     1381 aggcccatgt cggcttcccc ggccttgatc gccatggtga cgcagcggat cgcggtcgct
     1441 cctgtcgcgc aggcgttcgc gacgttgtac accgggattc cggtctgacc gatctgtttc
     1501 tggatccgct ggccgatgcc gctggccgcc tcgatgaggt tgccggcgcc catcacctcc
     1561 atgtcgtgga tcgtgacgcc accgtcgttc atcgcgttca tcgacgcttc cgacgccaag
     1621 tcgatcacgt ccttgtcggt gtaacgaccg aacttggtca tcgccgcacc gaggatccag
     1681 acgttgttat cggccatcac acgctccctt gggtcaggcg ggctcgtagg cgaacgcgac
     1741 cgcctcggtc ccattgtcgt cggtcccggc gacgtacgtg gtcagacgca ccggcaggcc
     1801 gaacgggacg tgggcggggt ccggctccac gttgagcagg ttggctttca ctgacccgcc
     1861 cccgtcgagg tccacgaccg ccgagacgta cggcgtcggg acgcccggcg aggcccggtg
     1921 cacgatcgtg aaggtccgga cccggccggt gtcggcgagc cggcgggtcg tgaagcccgg
     1981 cttcccggac ttggcgtcgg cgttgcgccg gtcgaagtag agggcgccgc tctcgacgga
     2041 ctcgtgggcg atgaggtggg gttcgccgtc gtcgatgacg aggtactcga cgagcgggac
     2101 ccgggcgggg gaagtctcag acacgccggg gcacgttagt cggaggtgtc cccagcgcga
     2161 gaatgtcgcg cgcgagcgtg gcggaacggc agacgcgccg ggtttaggtc ccggtgccct
     2221 tcggggtgtg ggggttcgac tccccccgct cgcacttcgg tcgggtatcc cgtggaccgg
     2281 gggccgcatc gcccgtctgc cggcgacggt cagcggcgtt tgcggcgccg gcggcgtttc
     2341 ttgccgtcga aatcggccag gatgtcagga gcggcgctgt tcacgatctc ctgggcggcg
     2401 tcgagcaagg cggcgacgtc gtcgccggcg ggttcggtct cggatcctcg cccggctgtg
     2461 ctgaggctgc cgtagtgcca gccggcgtcc atgcgccgct tggtgaagct ggggcactcc
     2521 tcggggcagc gccacggcgc ctcgggcgcc aggttgagtt tgcagcgatg cacgacctca
     2581 cccgagccgt aggtccgggt ctcgtaatgg cgacagtcgc tgcgcatcgg cacggaccca
     2641 ttgtggtccc tcgacggccg gtacctctcc cgcggaccca ggttccggtc ccggctgtgg
     2701 ccctagcgtg ggggctggtg cggcccgacc cctgcggggt cggtgctcag ggcaccgagg
     2761 accggcgcct gtgccggggt tctcgggcgc cgacctgagc tccgtgatcg gcacctcgcc
     2821 cgtctcaggt cggttcgatt cggcgccgat catcttcggg atgggaccgg gaatggcgag
     2881 gacaggacca cgatgacacc cagcgggcag gcccggcccg ccgatggccc cagcacgaac
     2941 cgccccgacc tgccggccgg tctcgtcggc ctcgtcgggc ggacgccgga catggtcatc
     3001 gcgatcgacg cccgcgggac gatcgtgtcg gccaacgcgg ccaccgagaa ggtgctgggc
     3061 cacgccgcgt cgacgaaggt ggggaccgcg gtcctcgatc tcgtccatcc ggacgaccgc
     3121 gcccgcgccg aagccggtct caaggccttg acgaccggtg gcgacgcgat cgccccggtc
     3181 ttggttcggg ctcgctgcgc cgatggcagc tggctgccca tcgaggtcac cgccacgaac
     3241 ctgctcgagg agccgacggt gcaggcaatc gtgctcatcg cccgagacct gagcgaacgc
     3301 cgccgcctcg aacgccgggt ccatgagctc gagcacggct tcaccgccgc gttccgtcac
     3361 tcccccgtcg ggcgcaccct cgtggatctg cggggccgat ggctgttggt caacgacgcg
     3421 ttcgcccgca tggtgggccg tgacgccaac agcctgatcg ggacccgggg gagcgacgtc
     3481 atccaccccg acgaccgagc ccgcgtccga gccgagctcg cctcgctcgc ggctcgcgcc
     3541 gtcgatagca cgacggtcga gacccggttc atccggtccg acggggacac ggtctgggcc
     3601 cggttcacgg tctggctggt attcgacgac gacggggagc cgacgcacct cgggtccgat
     3661 gtcaccgacg tcaccgagct ccgcgccgcc cgggacgtcg aagaccgtgt ccgccgcggg
     3721 ttcgaagccc tcgtcgagca ctcctcggac atcatcaccg tgctggagcc cgacggcacc
     3781 tggcgatcat cgagcgcggc cgggtcccgc ctcctcggct acgaaagggg tttcgacccc
     3841 gacgggggca tcttggcgct ggtgcacccc gacgatttcg agatgacggg tcgggcgctc
     3901 gacgaggtcc ggcgaggcac gcgaggaccg aatgagtcgc tggtggtgcg tatccgcgcc
     3961 gcggacggct cgtaccac
//
LOCUS       DATCCH010000041         2674 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_417120, whole genome shotgun sequence.
ACCESSION   DATCCH010000041 DATCCH010000000
VERSION     DATCCH010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417120"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VIJ44_00920"
     CDS             complement(<1..345)
                     /locus_tag="VIJ44_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164488.1"
                     /translation="MRRQAVAALAAVTLGAPMAVVLTATGVWATAVTKATPTVAVCNP
                     KTAAKGIAAAFNQFLAAPTALEKVRFVDQGTKVEAAVGKSDQVTLTSGRSNPPLQDVA
                     VNLEATCTAKTKA"
     gene            complement(381..1073)
                     /locus_tag="VIJ44_00925"
     CDS             complement(381..1073)
                     /locus_tag="VIJ44_00925"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY5164489.1"
                     /translation="MARRNGAAARQAGRGPDGPVQGRELRARGRRTLGRLLDAGATVF
                     ADRGFHAARVDDIVKAARTSHGTFYLYFASKDDLFRALAEDVAAAMVALARELPPLTP
                     DRDGYESLVAWLRRFTDLYRRHGALIRTWTEAEIVDSELGQVGSDLVAQFALELATRL
                     RVAAPDVDARVGALALVAMIERANYYAEARQIRVDRDDLVTTLAAVTHAGLFGVAARS
                     AQPAAVTPVLRS"
     gene            1143..1562
                     /locus_tag="VIJ44_00930"
     CDS             1143..1562
                     /locus_tag="VIJ44_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013423996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEY5164490.1"
                     /translation="MTTSAAPFDTLTVGDEAPPLIVENLTRTNFVRYAGASGDFNPMH
                     HDDTIATSVGNPSVFGHGMLTAGLMARVLKDWFGPTALRRFQVRFAKQVWPGDTLTCR
                     ATVTGKHDEERLVDIECSVTNQDGVEVLTGTATATAS"
     gene            1573..2478
                     /locus_tag="VIJ44_00935"
     CDS             1573..2478
                     /locus_tag="VIJ44_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009718941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5164491.1"
                     /translation="MGLLDGRVAIVTGSGRGIGREFALAMARAGARVVVNDIGATLDG
                     TGTGEDPATQVCREIESFGGEAIPNHDSVTDFDAASRIVASAVDAFGQLDILVNNAGI
                     VRDRTLVKMNAEDFDAVLAVHLKGSFNCARHAAVVMKDAGHGRIINITSSAGLRGNFG
                     QTNYGAAKAALMGVTFTWALELGRYGITVNAVAPAGATRMTAALFERSGEQPPPEQDP
                     ALNAPLVVFLASDRAGHVNGQLLGRTDYAYTLFQHPKQIAFMWRDGGWTPELVAEHFD
                     QVLGQHLQTVGMVMPSGMEFSGGTA"
     gene            2481..>2674
                     /locus_tag="VIJ44_00940"
     CDS             2481..>2674
                     /locus_tag="VIJ44_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164492.1"
                     /translation="MSFPLGVVDLAFRPDPPEASAERAASLGFDHIDVVVDVDPSTLV
                     LPVGCPTAFPKPQPGWCSTPA"
BASE COUNT          407 a          974 c          899 g          394 t
ORIGIN      
        1 cgccttcgtc ttggcggtgc aggtcgcctc gaggttgacg gcgacatcct gcaacggtgg
       61 gttcgagcgg ccggacgtca gggtcacctg gtcgctcttg cccaccgcgg cttcgacctt
      121 ggttccctga tcgacgaacc tgaccttctc gagcgcggtc ggggcggcga ggaactggtt
      181 gaacgcggcg gcgattcctt tggccgcggt cttggggttg cagaccgcca ccgtgggcgt
      241 cgccttcgtg accgcggtgg cccaaacccc ggtcgcggtc agcacgacgg ccatcggggc
      301 tccgagcgtg acggccgcga gggccgcgac ggcctgcctc ctcaccgcac ccccttgcca
      361 atcccggtga gacgccgggc tcaggaacgt aacaccgggg tcacggccgc tggctgcgcg
      421 ctccgagcgg cgacgccgaa cagccccgcg tgggtcaccg cggcgagcgt cgtgaccaga
      481 tcgtcccggt cgacgcggat ctggcgggcc tcggcgtagt agttggcccg ctcgatcatc
      541 gcgaccagcg ccagggcacc cacccgggcg tcgacatcag gggcggcgac ccgaagccgg
      601 gtcgcgagct ccagcgcgaa ctgcgccacg aggtcgctgc cgacctgtcc gagctcgctg
      661 tcgacgatct cggcttcggt ccaggtccgg atcagcgcgc cgtgtcggcg gtacaggtcc
      721 gtgaaccgac gaagccacgc cacgagcgac tcgtacccgt cgcggtccgg ggtgaggggt
      781 ggcagctcgc gggcgagcgc caccatcgct gccgcgacat cctcggcgag cgcacggaac
      841 aggtcgtctt tgctcgcgaa gtacaggtag aaggtcccgt gggatgtccg agccgccttc
      901 acgatgtcgt cgactcgcgc cgcgtggaag ccccggtcgg cgaacaccgt cgcaccggcg
      961 tcgagcagcc ggcccagggt tcgccggccg cgagcacgca actcccgacc ctgcaccggg
     1021 ccgtccgggc cccgacccgc ctgtcgcgcc gcggcaccgt tccggcgagc catgtggacc
     1081 ggagcgtaga agagcctgac ggtcgtgtca ccttttcctg ggctcgcgat acgctccgcg
     1141 ccatgaccac gagcgcagcg ccgttcgaca ccttgaccgt cggcgacgag gcaccacccc
     1201 tcatcgtcga gaacctcacc cgcaccaact tcgtgcggta cgcgggcgcg tcgggagact
     1261 tcaacccgat gcaccacgac gacacgatcg cgacctcggt ggggaacccg tcagtgttcg
     1321 ggcacggcat gctcaccgcc gggctcatgg cccgggtcct caaggactgg ttcggcccga
     1381 ccgcgctgcg gcgcttccaa gtccggttcg cgaagcaggt ctggcccggt gacacgctca
     1441 cctgccgggc caccgtgacg ggcaaacacg acgaggaacg cctcgtcgac atcgaatgca
     1501 gcgtcaccaa ccaggacggc gtcgaggtcc tcaccgggac cgccaccgcg accgcgagct
     1561 gaggcgggcg cggtgggtct cctcgacggc cgggtcgcga tcgtcaccgg ctcgggacga
     1621 ggcatcggac gcgagttcgc gctggccatg gcccgcgccg gcgcccgggt cgtggtcaac
     1681 gacatcggcg cgaccctcga cggcaccggt accggcgagg acccggcgac gcaggtgtgt
     1741 cgcgagatcg agtcattcgg gggcgaagcg atcccgaacc atgactcggt caccgacttc
     1801 gatgccgcca gccgcattgt ggccagcgcg gtcgacgcgt tcggccagct cgacatcttg
     1861 gtgaacaacg ccgggatcgt gcgggaccgc accctcgtga agatgaacgc cgaggacttc
     1921 gatgccgtcc tcgcggtgca cctcaaggga tcgttcaact gcgcccggca cgccgccgtg
     1981 gtgatgaagg acgccggcca cgggcggatc atcaacatca cgtcctccgc cggtctccgc
     2041 ggcaacttcg ggcaaaccaa ctatggggcg gcgaaggccg cactgatggg cgtgacgttc
     2101 acctgggcgc tcgagctcgg tcgctacggc atcaccgtca acgcggtcgc ccccgcgggt
     2161 gcgacccgga tgaccgccgc gctgttcgag cgctcggggg agcagccgcc accagagcag
     2221 gacccggcgc tgaacgcgcc gctggtcgtg ttcttggcgt ccgaccgggc cggtcacgtc
     2281 aacgggcagc tccttggccg caccgactac gcctacacgc tgttccagca ccccaagcag
     2341 atcgccttca tgtggcgcga cggcgggtgg acccccgagc tcgtcgccga gcacttcgat
     2401 caagtgctcg gccagcacct ccagaccgtc ggcatggtga tgccatccgg gatggagttc
     2461 tcgggcggaa ccgcataggc gtgtcgttcc cgctcggcgt tgtcgacctc gcgttccgcc
     2521 ccgacccacc cgaagcgagt gccgagcgcg ccgccagcct cggtttcgac cacatcgacg
     2581 tcgtcgtcga cgtcgaccca tccacactcg tgctccccgt cggttgcccg accgcgttcc
     2641 cgaagccaca acccggctgg tgctccacgc ccgc
//
LOCUS       DATCCH010000042         2178 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_295363, whole genome shotgun sequence.
ACCESSION   DATCCH010000042 DATCCH010000000
VERSION     DATCCH010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295363"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..706
                     /locus_tag="VIJ44_00945"
     CDS             116..706
                     /locus_tag="VIJ44_00945"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEY5164493.1"
                     /translation="MPSRRILTIPNVISVGRLACAPVFCWLLFDDHRVAAFVLLGTLG
                     ATDWVDGWIARRFDQGSDLGKILDPVADRILLVTAAVALLIDGTVPAVIGALVLVREG
                     LVAAATLGLAAAGARRIDVQWVGKAGTLALMFALPGFLLIDVVGAGTAQDVIRVLTWC
                     FTVGGLALSYYAVVAYVPLARAALAEGRQARAGVTA"
     gene            703..>2178
                     /locus_tag="VIJ44_00950"
     CDS             703..>2178
                     /locus_tag="VIJ44_00950"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEY5164494.1"
                     /translation="MKAVILAGGEGTRLRPLTSNQAKPMMPLVNKPMMEHIVGLLAQH
                     GFDDVVVTVAYLANQIRTYFGDGSDFGVRIRYATDEEPLGTAGSVRNAAAELDDTFLV
                     ISGDVLTDIDLTAFVKTHRDSGAIASIALKRVENPLEFGIVITRPDGSIERFLEKPSW
                     GQVFSDTINTGIYVLEPAIFDSIPEGEVIDFAGDVFPAVLDQGQPLHGHITDGYWEDV
                     GTTEAYLRAHADVLDGRVLVDIGGFEIRDGVWLGEGAEIDPDTRIDGPVVLGDNCRIE
                     AGAHLSEYTVLGADVVVKPEASIVRTVLHDHVYVGRAAQLRGAVVGRNSDLREHVRLD
                     EGVVLGDECFIGRHAVISAGVKVYPFKTVEADAVVNSSIVWETRGVRTLFGPHGVRGL
                     ANVDVTPEMAVRVAMAYGTALKRGSFVTASRDTSRAARALKRAVIGGLNLAGVTVEDV
                     ELATVPLTRFQVRNSHALGGISVRLVPGDPDSVELRFFDADG"
BASE COUNT          310 a          768 c          753 g          347 t
ORIGIN      
        1 atgaccggat ccgaccaatt ctcgccgccc gggctgcggt gcagctcgag ttcgctccct
       61 gaccgcgagt tcgcgccctg accgggggcg ggaggccctc gcgtacgctg ggtgcgtgcc
      121 cagccggcga atcctcacga ttcccaacgt gatctcggtc gggcgcctgg cgtgcgcgcc
      181 ggtgttctgc tggctgctct tcgacgatca ccgggtcgcg gcgttcgtgc tgctcgggac
      241 cctcggcgcc accgactggg tcgatgggtg gatcgcccgc cgcttcgacc agggcagcga
      301 cctcggcaag atcctcgacc cggtcgcgga ccggatcctc ctcgtcaccg ccgccgtggc
      361 gctgctcatc gacgggaccg tgcccgccgt gatcggcgcg ttggtcctgg tccgagaggg
      421 actggtcgcc gccgcgaccc tcgggctcgc cgcggcgggg gcgcgtcgca tcgacgtgca
      481 gtgggtcggg aaagccggga cgttggcgct catgttcgcc ctgcccggct tcctcctcat
      541 cgatgtcgtc ggggccggca ccgcccagga cgtcatccgc gtcctgacct ggtgcttcac
      601 cgtcggcggg ctcgccctca gctactacgc ggtcgtcgcc tacgtgccat tggcgcgcgc
      661 cgcgctcgcc gaggggcgcc aggcccgggc cggggtcacg gcatgaaggc ggtgatcctc
      721 gccggtgggg aagggacccg gttgcggccc ctgaccagca accaggcgaa gccgatgatg
      781 ccgctggtca acaagcccat gatggaacac atcgtcgggc tgctcgccca gcacggcttc
      841 gacgacgtcg tcgtcactgt cgcgtacctc gccaaccaga tccgcaccta cttcggcgac
      901 ggatccgact tcggggtgcg gattcggtac gccaccgacg aggagccgct cggaacggcc
      961 gggtcggtgc gcaacgccgc cgcggagctc gacgacacgt tcctcgtcat ctccggcgat
     1021 gtgctcaccg acatcgacct caccgcgttc gtgaagactc accgcgacag cggcgcgatc
     1081 gcatcgatcg cgttgaagcg ggtcgaaaat ccgctcgagt tcgggatcgt cattacccgg
     1141 cctgacggga gcatcgagcg gttcctcgag aagccgtcct ggggccaggt cttctcggac
     1201 accatcaaca ccggcatcta cgtgctcgag ccggcaatct tcgacagcat ccccgaaggt
     1261 gaggtcatcg acttcgccgg ggacgtgttc cccgcggtgc tcgaccaggg tcaaccgctg
     1321 cacggtcaca tcaccgacgg gtactgggag gacgtcggaa ccaccgaggc gtacctacgg
     1381 gcccacgccg acgtgctcga cggccgggtc ctggtcgaca tcggcggctt cgagatccgc
     1441 gacggcgtgt ggctcggcga gggcgccgag atcgaccctg acacccgcat cgacgggccg
     1501 gtcgtcctcg gcgacaactg tcgcatcgaa gccggcgcgc acctctcgga gtacaccgtc
     1561 ctcggagcgg acgtggtcgt gaaacccgag gcgtcgatcg tccggaccgt gctccacgac
     1621 cacgtctacg tcgggcgggc agcgcagctg cgcggcgcgg tggtcggacg taacagcgac
     1681 ctgcgggagc acgtccgcct cgacgagggg gtcgtcctcg gcgacgagtg cttcatcggc
     1741 cgccacgcgg tgatcagcgc cggggtgaag gtgtacccgt tcaagaccgt ggaggccgac
     1801 gctgtcgtca actcgtcgat cgtgtgggag acccgcgggg tccgcaccct gttcggcccg
     1861 cacggcgtcc gtgggctcgc caacgtcgac gtcacgcccg agatggcggt gcgggtcgcg
     1921 atggcgtacg gcacggcgct gaagcggggc tctttcgtga ccgccagccg ggacaccagc
     1981 cgcgccgcgc gggcgcttaa gcgggcagtg atcggcggcc tcaacctggc cggggtcacc
     2041 gtcgaggacg tggaactcgc caccgtcccg ttgacccgct tccaggttcg caacagccac
     2101 gcgctcggcg gcatctcggt gcggctcgtc ccgggcgatc cggacagcgt cgagctgcgc
     2161 ttcttcgacg ctgacggc
//
LOCUS       DATCCH010000043         2371 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_127001, whole genome shotgun sequence.
ACCESSION   DATCCH010000043 DATCCH010000000
VERSION     DATCCH010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127001"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..1521
                     /gene="crtI"
                     /locus_tag="VIJ44_00955"
     CDS             52..1521
                     /gene="crtI"
                     /locus_tag="VIJ44_00955"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020489188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HEY5164495.1"
                     /translation="MRVAIVGAGLGGLAATCHLAGRGHEVVVFEREPVPGGVAGMLEG
                     SGYRVDTGPAVLTMLGILEATFNAAGATLDDHLTVQRLDPMYRACFADGSELLVRAGI
                     DAMAAEIEQFAGLKDADGFRRFTEWLRDLYDAEFGPFINRNFDSPLDLAARPLELARL
                     VRLGALGRLAPKVASFFGDERLQRVFSFQALYAGLSPFDALAVFSIITYMDTIEGVSF
                     PSGGMHEVARGLAAAAEKAGATFAYSCPVDRVDRGPDGVVNGVRLDTGEHVAADAVIC
                     NADVASTYRTLLGIPAPRLVARGQYSPSCALWLAGVRGPAPSGAAHHNLHFGRPWREA
                     FDTLLRDGRRMPDPSILVTTATVTDPTLAPDGGTTLYVLEPVPNLDGRIDWDADGPGV
                     RADLIARVGNLGYPVDDVELEQFLDPAGWAARGLERGTPFALAHRFFQSGPFRTSNVD
                     RRVPGLVLVGMGTVPGVGIPMVLLSGRLAADRVEGLDGS"
     gene            1518..>2371
                     /locus_tag="VIJ44_00960"
     CDS             1518..>2371
                     /locus_tag="VIJ44_00960"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020516910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HEY5164496.1"
                     /translation="MTTLDESYARCRHLNKAYGTTYYVATLLMPRVKRHHVHALYAFC
                     RYADDIVDDLGLAPVEEREHALTEFGDRFFTDLERGDSDDTVLKAVVHTVNAFDLNPD
                     CFRRFLRSMTMDLTVETYESFDDLLDYMDGSAAVIGEMMLPILEPTSAAALRHARDLG
                     LAFQLTNFLRDVDEDLDRGRVYIPQEDLRRFDADPWQRRVTPEWRALMAFEIRRTHEY
                     YESADLGIPLLPPSSARCIRAARRLYSEILDRIERAGGDVFTKRARVPTARKALVVGS
                     SLLNLGGG"
BASE COUNT          339 a          846 c          772 g          414 t
ORIGIN      
        1 actccaggag ctcggcacgt acgccgcctg gcgcgaccgg taacgagacc catgcgggtc
       61 gcgatcgtgg gggcgggcct cggcgggctc gccgcgacgt gtcatctcgc cgggcgaggc
      121 cacgaggtgg tcgtcttcga acgagagccg gtccccggcg gtgtcgccgg gatgctcgaa
      181 ggcagcggct accgggtcga caccggcccc gccgtcctca ccatgctggg catcctcgag
      241 gccaccttca acgctgccgg cgcgaccctc gacgaccacc tgaccgtcca gcggctcgat
      301 ccgatgtacc gcgcctgctt cgcggacgga tccgagcttc tggttcgagc gggcatcgac
      361 gcgatggcgg ccgagatcga gcagttcgcc ggactcaagg acgcggacgg attccgtcgg
      421 ttcaccgagt ggcttcgcga cctctacgac gccgagttcg ggccgttcat caaccgcaac
      481 ttcgattcgc cgctcgacct cgccgctcga cctctcgagc tggctcgcct cgtgcgcctc
      541 ggcgcgttgg gccggctcgc accgaaggtc gccagcttct tcggcgacga gcgcctgcag
      601 cgggtgttct cgtttcaggc gctgtatgcg ggcttgtcgc ctttcgacgc gctcgcggtc
      661 ttcagcatca tcacgtacat ggacaccatc gagggggtct cgtttccttc cggtggcatg
      721 cacgaagtcg cccgtggtct cgctgccgcc gcggagaagg ccggcgcgac cttcgcctac
      781 tcgtgcccag tggatcgcgt cgaccgcggt ccggacggtg ttgtcaacgg cgtccgcctc
      841 gatacgggag agcatgtcgc cgcggacgcg gtgatctgca acgccgacgt cgcgtcgacg
      901 taccggactc tgctcgggat ccctgcgcct cggctcgtgg cgcgcggcca gtactcaccg
      961 tcctgtgccc tctggctcgc cggggtccgg ggtccggccc cgtccggagc ggcgcaccac
     1021 aacctccact tcgggcgtcc gtggcgtgag gccttcgaca cgctcttgcg cgacggccgg
     1081 cgcatgccgg acccgtcgat cctcgtcacg acggcaaccg tcaccgaccc gaccttggct
     1141 cccgatggcg ggacgacgct gtacgtcctc gaaccggtgc cgaacctcga tggtcggatc
     1201 gactgggacg ccgacgggcc gggcgttcga gccgacctga tcgcgcgggt cgggaacctc
     1261 ggctacccgg tcgacgacgt cgagcttgaa cagttcctcg atccggccgg atgggcggcg
     1321 cggggcctcg agcggggaac gccgttcgcg ctggcacacc ggttcttcca aagcggtccc
     1381 tttcggacct ccaacgtcga ccgccgcgtc ccgggacttg tcctcgtcgg gatgggtacg
     1441 gtgccaggcg tgggaatccc catggtgctg ctctcgggtc gcttggccgc ggaccgggtc
     1501 gaagggttgg acgggtcgtg accaccctcg acgagtccta cgcgcgctgt cgccacctca
     1561 acaaggcgta cggcacgacc tactacgtag cgacgctgct gatgcctcgc gtgaagcgac
     1621 atcacgtcca tgccctctat gcgttctgcc gctacgccga cgacatcgtc gacgacctcg
     1681 ggctggcacc ggtcgaggag cgtgagcacg cgctcaccga attcggagat cggttcttca
     1741 ccgacctcga gcgcggcgac tccgacgaca cggtcttgaa ggcggtcgtg cacaccgtca
     1801 acgccttcga tctcaatccg gactgcttcc gtcggttcct ccgctcgatg acgatggacc
     1861 tcaccgtcga gacctacgag tccttcgatg acctgctcga ttacatggac ggatcggccg
     1921 cggtgatcgg ggagatgatg ctccccatcc tggaacccac ctcagccgcg gcgctccgtc
     1981 acgcgcgcga tctcggactg gcgttccagc tgacgaactt tctgcgcgac gtcgacgagg
     2041 acctggatcg aggccgcgtc tatatccccc aggaggatct gcgacgcttc gacgcggacc
     2101 cgtggcagcg gcgcgtcacg cccgagtggc gcgccctcat ggcattcgag atccgccgca
     2161 cccacgagta ctacgagtct gccgacctcg ggatcccgct gctgccccca tcctcagcgc
     2221 gctgcatccg cgcggcgcgt cgtctctact ccgagatctt ggatcggatc gaacgtgccg
     2281 gcggcgacgt gttcaccaag cgcgcccggg tcccgacggc ccgcaaggcc ctcgtcgtcg
     2341 ggagcagcct cctcaatctc ggtggtggtg a
//
LOCUS       DATCCH010000044         2320 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_127018, whole genome shotgun sequence.
ACCESSION   DATCCH010000044 DATCCH010000000
VERSION     DATCCH010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127018"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..167
                     /locus_tag="VIJ44_00965"
     CDS             24..167
                     /locus_tag="VIJ44_00965"
                     /inference="COORDINATES: protein motif:HMM:NF019044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164497.1"
                     /translation="MHAKGDVAIRGPASDLLLVMMRRRSPDDVEVLGDRAVLDGFLDQ
                     AGF"
     gene            230..958
                     /locus_tag="VIJ44_00970"
     CDS             230..958
                     /locus_tag="VIJ44_00970"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEY5164498.1"
                     /translation="MHVLLGIVVGLVTAELAILITTVYLHRGLTHRAITVHPVVAGPL
                     RVVIWVATGMKPREWAAVHRRHHAATDTPDDPHSPLVLGFWRVQLANAALYRRTDRDG
                     VTVDRYAKDLPPDRLDRWFFDRAWLGLGIGVVVLCLVLGWQTGLLAAVVHMVAYLGLS
                     GSINAIGHTVGDRPHANSATNGRFLALITAGEGLHNNHHDAPTSARFARKRGDLDPGW
                     WAVRSLSLLGLVRIRHRKLPARSS"
     gene            complement(983..1960)
                     /locus_tag="VIJ44_00975"
     CDS             complement(983..1960)
                     /locus_tag="VIJ44_00975"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016828285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEY5164499.1"
                     /translation="MSVPARYGITVPFTDVSLADHRSWYEEVAALGYTDVWSAEAGAT
                     DAFTPLALAAAWTPQLRLGTAIVPVFTRGAATLAQSVAAMCQAAPGRFALGLGTSSEV
                     IVERWNGIPFVKPYHRVRDVVRFLRAALPGDKVTAEYETFSVRGFRLEVPVAEMPPIL
                     IAALRPGMLRLAGREGDGAIINWLSADDVKTVVPHVGEDKEIVARIFVLPSEDRDLVR
                     FVGRRAIAAYLNVPVYAAFHEWLGRGEQLAPMWAAWRAGDRAAATEAIPDSLVDELIV
                     HGSPSECREHLARYAANGVTTPAPAILGLGVDVLRQAVRDLAPSASPAD"
     gene            complement(1957..>2320)
                     /locus_tag="VIJ44_00980"
     CDS             complement(1957..>2320)
                     /locus_tag="VIJ44_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164500.1"
                     /translation="LWVRLPGIPATASALAVVADYVPFGISQALGRRTSSNSLDNTLR
                     MVRPLDLHPTEWVLADVRVQAVADGFGHGVVHLWAEDGTLLATASQSAIVRNWASREA
                     RAAEPAPKATDLSSEASP"
BASE COUNT          340 a          895 c          752 g          333 t
ORIGIN      
        1 ccgacggcct cgaggtcgag gcgatccacg ccaagggtga cgtcgcgatc cgcggcccgg
       61 cgtcggacct gctcctcgtc atgatgcgtc gacgatcccc cgacgacgtc gaggtcctcg
      121 gcgatcgggc cgtcctcgac ggcttcttgg accaggccgg cttctaagtt caccctctgg
      181 ccatcggctc attcggcgcc gggacgcccc ggcccgctac tttgcagttg tgcacgtact
      241 actgggcatt gtcgtcgggc tcgtgaccgc cgagctggcg atcctgatca ccaccgtcta
      301 cctgcaccgg gggctcaccc accgggcgat caccgtccat cccgtggtcg ccgggccgct
      361 gcgcgtcgtc atctgggtcg ccaccggcat gaagccccgc gagtgggccg cggtccaccg
      421 gcgccaccac gccgccaccg atacccccga cgacccccac tcgccgctcg tcctcggttt
      481 ctggcgggtg cagctcgcca atgccgcgct ctaccgtcga accgaccgcg acggcgtcac
      541 cgtcgaccgc tacgccaagg acctgccgcc cgatcgcctc gaccgctggt tcttcgaccg
      601 ggcgtggctc ggactcggca tcggcgtggt cgtcctgtgc ctcgtcctcg ggtggcagac
      661 cgggctcctc gccgcggtgg tgcacatggt cgcctacctc ggcttgagcg gctccatcaa
      721 cgccatcggc cacaccgtcg gcgatcgacc ccacgccaac tcggcgacga acggacggtt
      781 cctcgccctg atcaccgccg gtgagggtct gcacaacaac caccatgacg cccccacctc
      841 ggcacgcttc gcccgcaaac gaggggacct cgaccccgga tggtgggcgg tgcgctcgct
      901 ctcgcttctg gggctggtgc gcatccggca ccgcaagctc ccggcccgtt ccagctgacg
      961 ccgcgggcgc ggcgagcctc gctcagtcgg cggggctggc gctcggcgcc aggtcacgca
     1021 ccgcctggcg cagcacgtcg acgccgagcc ccaggatcgc cggcgccgga gttgtgaccc
     1081 cgttcgccgc gtaccgggcc aggtgctcgc ggcactcgct cggcgatcca tgcacgatga
     1141 gctcgtcgac gagcgagtcg gggatcgcct cggtcgccgc ggcgcgatca ccggcccgcc
     1201 acgccgccca catcggcgcc agctgctccc cacgtcccag ccactcgtgg aaggcggcat
     1261 agaccgggac gttgaggtag gccgcgatgg cccgacggcc gacgaaacga accaagtccc
     1321 ggtcctcact cggcaggacg aagatgcgcg ccacgatctc cttgtcctca ccgacgtggg
     1381 ggacgacggt cttgacgtcg tcggccgaca accagttgat gatcgccccg tcaccctctc
     1441 ggcccgcgag gcgcaacatt ccgggccgga gcgccgcgat gaggatcggc ggcatctcgg
     1501 ccaccggcac ctcgaggcga aagccgcgca ccgagaacgt ctcgtactcg gcggtcacct
     1561 tgtcgccggg gagcgcggcc cgcaggaagc gcacgacgtc gcgcacccgg tgatacggct
     1621 tcacgaacgg gatcccgttc caccgctcga cgatgacctc cgacgacgtc cccagcccga
     1681 gcgcgaagcg tcccggcgcg gcctgacaca ttgccgctac cgactgggcc agggtcgcgg
     1741 ccccccgggt gaacaccggc acgatcgcgg tcccgagccg cagctgcggc gtccaggccg
     1801 ccgccaacgc gagcggcgtg aacgcgtccg tcgcccctgc ctccgccgac cacacgtcgg
     1861 tgtagccgag ggccgcgacc tcctcgtacc agctgcggtg atctgccaac gacacgtcgg
     1921 tgaacgggac cgtgatgccg tatcgagccg gaacactcat ggggacgcct ccgaggagag
     1981 atcggtcgct ttcggtgctg gttccgccgc gcgggcctcc ctgctggccc agttccgcac
     2041 gatcgcggac tggctggcgg tggcgagcag cgtgccatcc tcagcccaca ggtgcacgac
     2101 cccgtgtccg aacccgtccg caaccgcctg gacccgcacg tcggcgagga cccactcggt
     2161 ggggtggagg tccaagggac ggaccatccg caacgtgttg tcgagactgt tgctcgacgt
     2221 gcgccgaccc aacgcctggc tgatcccgaa cgggacgtag tcggcgacca ccgcgagcgc
     2281 cgacgccgtc gccgggatcc ccggcagccg gacccacaac
//
LOCUS       DATCCH010000045         2241 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_88611, whole genome shotgun sequence.
ACCESSION   DATCCH010000045 DATCCH010000000
VERSION     DATCCH010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88611"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="VIJ44_00985"
     CDS             complement(<1..687)
                     /locus_tag="VIJ44_00985"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEY5164501.1"
                     /translation="MRGVVARRQRLVVEDLPDPTPAAGEALVALRACGICGSDLHTLA
                     HAEVLPEVATAMGLATTFDPDGDYYLGHEWVGEVLDLGPDTADAPVSPGDRVVSMPYL
                     VRGPDLVPLGFSNDHYAGYCERFLLTAALCLRVPNGLDVRLAALTEPMAVGLHAVNKA
                     GLTRGDTALVVGCGPIGLALIAWLRTRGVEPIVACDYSPQRRALAERVGAHVVVDPRD
                     QPGIDAWRAHA"
     gene            759..2003
                     /locus_tag="VIJ44_00990"
     CDS             759..2003
                     /locus_tag="VIJ44_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020544463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY5164502.1"
                     /translation="MSIDFTLPADVEAVRLRIREFMDAEVRTAEEKLYANAPGGEPDR
                     REVVAMIIDLRQKAKEWGVWLPHMPTEWGGLGLGITAMAFVSAEAARTNFGPFVLNAQ
                     APDEGNQHTLLHHGNPDQQERYLRPSVEGRVRSCFAMTEPEVAGSDPTGIRTTAVLDD
                     ATGDWIVNGHKWFISGARGAGFAILIAKTDPDADPPQASNTAFLVDLPSDGWEVVRDV
                     ETMAGHGNHCEIRITDLRVPAANVLGGRGNGHLLGQARLGPARLAHCMRWIGQIETAF
                     EMLVDRAEKRQLHGEPLAEKQAIQWMMADSAMELYSAKLMVLHAAYKIEHGLPFRQEV
                     SFAKHHVANTLWRVVDRAVQVHGALGYSTDTPLERMLRHARSARLVDGADEVHQALIA
                     RNVRGAYAESGSIKAATGDLEL"
BASE COUNT          363 a          776 c          803 g          299 t
ORIGIN      
        1 cgcgtgcgcg cgccacgcgt cgatgccggg ctggtcgcgg gggtcgacga cgacgtgcgc
       61 gccgacgcgc tcggcgaggg cccgccgttg cggtgagtaa tcgcacgcca cgatgggctc
      121 gacgccgcgg gtgcggagcc aagcgatgag ggccagcccg atggggccac agcccacgac
      181 cagggcggtg tcgccccggg tgaggccggc cttgttgacc gcgtggaggc cgacggccat
      241 gggctcggtc agcgcggcga ggcgcacgtc gaggccgttg ggcacccgga ggcacaacgc
      301 cgcggtcagc aagaagcgct cgcagtagcc ggcgtagtga tcgtttgaga atccgagcgg
      361 gaccaggtcg gggcctcgta ccaggtacgg catcgacacg acccgatccc cggggctgac
      421 cggggcgtcg gcggtgtcgg ggccgaggtc gagcacctcc cccacccact cgtgccccag
      481 gtagtagtcc ccgtcggggt cgaacgtggt cgcgaggccc atggcggtcg cgacctcggg
      541 gagcacctcc gcgtgggcca gggtgtgaag gtcggagccg cagatcccgc aggctcggag
      601 cgcgacgagg gcctcaccgg ccgccggggt cggatcggga aggtcctcga cgacgagtcg
      661 ttgacgtcgg gcgacgaccc ctcgcaccgg cgcagcgtac gcccgggcgt ggagccgtcg
      721 gtttgacccg gagcggggac cccacgacac tcgccgtgat gtcgatcgat ttcacgctcc
      781 ccgccgatgt cgaggcggtc cgcctccgga tccgcgagtt catggacgcc gaggtgcgaa
      841 cggcggagga gaagttgtac gccaacgccc ccggcggcga gcctgaccgt cgcgaggtcg
      901 tcgcgatgat catcgacctg cgccaaaagg ccaaggagtg gggagtgtgg ctcccccaca
      961 tgccgacgga atggggtggg ctcgggctcg ggatcaccgc gatggcgttc gtgtccgccg
     1021 aggccgcccg caccaacttc ggaccgttcg tgctgaacgc gcaagccccc gacgagggca
     1081 atcagcacac gctgctgcac cacgggaacc cggatcaaca ggagcgttac ctgcgaccct
     1141 cggtcgaggg tcgggtccgg tcgtgcttcg cgatgaccga acccgaggtc gcggggtccg
     1201 acccgaccgg gatccgcacc accgcggtgc tcgacgacgc caccggcgac tggatcgtga
     1261 atggccacaa gtggttcatc tccggggcgc ggggggccgg gttcgcgatc ctcatcgcca
     1321 agaccgaccc cgacgccgac ccgccgcagg cgagcaacac cgcgttcttg gtcgacctcc
     1381 cctccgacgg ttgggaggtc gtccgcgacg tcgagaccat ggccgggcac gggaaccact
     1441 gcgagatccg gatcaccgac ctgcgggtcc ccgcggccaa cgtcctcggc gggcgcggca
     1501 acggtcacct gctcggccag gcgaggctcg ggcccgcccg gctggcgcac tgcatgcgtt
     1561 ggatcggcca gatcgagacc gcgttcgaga tgctcgtcga ccgggccgag aaacgccagc
     1621 tccacggcga acccctggcc gagaagcagg cgatccagtg gatgatggcc gactcggcga
     1681 tggagctgta ctcggccaag ctcatggtcc tgcacgccgc gtacaagatc gagcacggcc
     1741 tgccgttccg ccaagaggtg agcttcgcca aacaccacgt cgcgaacacg ttgtggcggg
     1801 tcgtggaccg cgcggtgcag gtacacggcg cgctcggata ctcgaccgac acgccgctcg
     1861 agcggatgtt gcgccacgcg cgatcggctc ggctggtcga cggggccgac gaggtccatc
     1921 aggcgctcat cgcccgcaac gtgcgaggcg cctacgccga gagcggatcg atcaaggccg
     1981 ccaccggaga tctcgagctg taaacctccc caggccccat acgccgatgg ggtcgcgggt
     2041 cgaaccggcc ttcgcgcgga gtggttcgta cactcctggg atggtccaga ccgccgacgt
     2101 gctccgcgag gtgctgaaga ccccgcactg gtcgagcgag atcccgctcg acacgcccca
     2161 cccgagaccg gacgcgctca ccgagacggg ttacgtcgtg ctgcgcgacc tcgacccccc
     2221 gatcggcgaa cccgagtatt t
//
LOCUS       DATCCH010000046         2874 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_253786, whole genome shotgun sequence.
ACCESSION   DATCCH010000046 DATCCH010000000
VERSION     DATCCH010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253786"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /locus_tag="VIJ44_00995"
     CDS             <1..785
                     /locus_tag="VIJ44_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005061076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY5164503.1"
                     /translation="FADSNPSAVDFSSLRLVPCGGSAVPQALIEAFQERYGVEILQAW
                     GMTETSPVAAVAHPPKGIDLSDPDAIGWRVRAGRALPGVELRIVGDDGPVLWDDETMG
                     ELEVRGPWITGAYYRDATSDKFDDGWLRTGDIGTIDARGYVRITDRSKDVIKSGGEWI
                     SSVELENHLMAHPSVAEASVIGVPDARWEERPLACVVVVGGADLDAEDLREFLAARVA
                     KWQIPERWAFIPEVPKTSVGKFDKKVLRARHEAGQLDIVEVR"
     gene            complement(824..2152)
                     /locus_tag="VIJ44_01000"
     CDS             complement(824..2152)
                     /locus_tag="VIJ44_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164504.1"
                     /translation="MRRRLVFLGALALFAVVVAAGPAAAKGGGGGSHNRRISLSGALV
                     VATGEVVNGPVVSVDGPATIAGRVANDVFVGHGSLAISGHVTGDVLIVDGDATITGRV
                     DGDITALHGRVTVQTGGNVRGDITSSNAPRAAPGTVRGRVKTLDVTSIFTGFLVAFLI
                     ILWIAVTVSIGILGLLFVVVFPHGADAVVIAGRRVGVSIVAGLLVGILGPVLGVAIVS
                     TIVGIPLGIGLLAAMTVLAPLGYASSALILGRLMVRGPRARARVGAFFTGFAILRLVA
                     LIPGLGFIIWYLACFYGLGALTIAAWRAGHRETAAPVTAAPPSPVETPPTPASVPPEP
                     ATRAGTSSPRARRPTTATPARRRPTRAAPKKTTAKRTAKPSTTRKPVRTARAPAKKRT
                     GRPSATPRRAPAKAATRQSRPTAKRSTARAKRAAPRPAPRKAAGTARRSR"
     gene            complement(2248..>2874)
                     /locus_tag="VIJ44_01005"
     CDS             complement(2248..>2874)
                     /locus_tag="VIJ44_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011438239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEY5164505.1"
                     /translation="ERVAIDVPEEHLGVVTQLLASRKGRLEQMVNHGTGWVRMEYLVA
                     ARALVGFRTEFLTQTRGTGLLHHVFERYEPWLGELRTRATGSLVADRRGETTSYALMG
                     LQERGHMFVGPGLEVYEGMIVGENARAGDMDVNPTREKKLTNHRAAAADELVRLVPPR
                     PMSLEQALEFIASDEFVEATPISVRLRKATLSASTRGRRRGHDRAARH"
BASE COUNT          444 a          960 c         1047 g          423 t
ORIGIN      
        1 gcttcgcgga cagcaacccg tccgcggtcg acttctcgtc gcttcgactc gtcccctgtg
       61 gtggatccgc ggtcccccag gcgctcatcg aggcgttcca ggagcgctac ggcgtcgaga
      121 ttctccaggc ctggggcatg acggagacca gcccggtcgc ggccgtcgcc catcccccga
      181 agggaatcga tttgagcgac cctgacgcga tcgggtggcg ggtgcgcgcc gggcgcgcgc
      241 tgccgggggt cgagctccga attgtcggcg acgacggccc cgtcctgtgg gacgacgaga
      301 cgatgggcga gcttgaggtc cggggaccgt ggatcacggg cgcctattac cgcgacgcca
      361 cctccgacaa gttcgatgac ggctggttgc ggaccggaga catcggaacc atcgacgcac
      421 gcggatacgt tcggatcacc gatcgttcca aggacgtcat caagtccgga ggcgagtgga
      481 tctcatcggt ggagctggag aaccacctca tggcgcatcc gagcgtcgcg gaggcctccg
      541 tcattggggt gcccgacgct cgttgggagg agcgcccact tgcttgcgtg gtcgtcgtcg
      601 gtggcgccga cctcgacgcc gaggaccttc gcgagttcct cgcggcgcgc gtcgcgaagt
      661 ggcagatccc cgagcgttgg gccttcatcc cggaggtccc gaagaccagc gtcgggaagt
      721 tcgacaagaa ggtgcttcgc gctcggcacg aagccggcca gctcgacatc gtcgaggttc
      781 gctaagccag gccgaccccc gacccccgag ccccgggacg cggtcagcgg ctccggcggg
      841 cggtccccgc cgccttgcgc ggggcggggc gcggcgcggc gcgcttcgcc cgagccgtcg
      901 atcgtttcgc ggtgggacgg gattggcgag tcgcggcctt cgccggggcg cgccgcggcg
      961 tggcggacgg acgcccggtt cgcttcttcg ctggagcgcg agcggtgcgg accggcttgc
     1021 gggtggtgga cggcttcgcg gtcctcttgg ccgtcgtctt cttcggggcg gctcgggtcg
     1081 ggcgtcgacg ggccggcgtc gccgtggtcg gccgccgggc cctgggggag ctggtaccgg
     1141 ctcgggtcgc aggctcgggt ggaaccgagg ccggggtcgg aggcgtctcg acgggcgacg
     1201 gaggcgccgc ggtgaccggc gcggccgtct cgcggtgccc ggcgcgccaa gcggcgatgg
     1261 tgagcgcacc gagtccgtag aagcaggcca gataccagat gatgaagccc agtccgggaa
     1321 tcagcgcgac gaggcgcaag atggcgaagc cggtgaagaa ggcgccgacc cgcgcccggg
     1381 cgcgggggcc gcggaccatg agccggccga ggatcaaggc gctgctcgcg tagccgaggg
     1441 gcgccaggac ggtcatcgct gcgaggagtc cgatcccgag tgggatgccg acgatcgtcg
     1501 acacgatggc cacaccgagg accgggccca ggatcccgac caggagaccg gccacgatcg
     1561 acacgcccac gcggcgtccg gcaatgacca ccgcgtccgc gccgtgaggg aagaccacga
     1621 cgaagagcag ccccaggatg ccgatcgaca cggtgaccgc gatccataag atgatcagga
     1681 aggcgacgag aaacccagtg aagatgctcg tgacgtcgag ggtcttcaca cggccgcgta
     1741 ccgtgccggg ggcggctcgg ggcgcgttgc tcgatgtgat gtcgccgcgg acgttcccac
     1801 cggtctggac ggtgactcga ccgtgcaagg cggtgatgtc gccgtcgacc cggccggtga
     1861 tcgtggcgtc accgtcgacg atcagcacgt caccggtcac atgaccactg atggccagac
     1921 ttccgtgtcc gacgaagacg tcattggcga cgcggcccgc gatcgttgcc ggcccgtcca
     1981 ccgacaccac gggtccgttg acgacctcgc cggtcgcgac gaccagcgcg ccggagaggc
     2041 tgattcgccg gttatggctt ccgccgcccc cacctttcgc ggcggctgga ccggcggcga
     2101 cgaccacggc gaagagcgcc agcgcaccga ggaacaccaa tcgacgacgc atgagacccc
     2161 ttcagcccgt gggaggatcg aagcgtcgca ggtgccaggg cgtcgcgcaa cgccagcggg
     2221 cgccccgagc ccgatcgctc ggattcgtca gtgccgggca gcccgatcgt gaccgcgccg
     2281 gcggccacgc gtgctcgccg acagggtggc cttgcggaga cgcacgctga tcggggtggc
     2341 ttccacgaac tcgtccgagg cgatgaactc gagcgcctgt tccagggaca tcggacgcgg
     2401 gggaacgagt ctcacaagtt cgtcggctgc cgcggcgcga tggttggtca gcttcttctc
     2461 gcgggtcggg ttcacgtcca tgtcaccggc ccgcgcgttc tcgccgacga tcatgccttc
     2521 gtacacctcg aggcccggtc cgacgaacat gtgaccgcgt tcttggaggc ccatgagagc
     2581 atacgacgtt gtctctcccc gccggtcggc gacgaggctc ccggtcgcgc gggtgcgaag
     2641 ctcgccaagc cacggctcgt agcgctcgaa gacgtggtgc aggagaccgg ttcctcgggt
     2701 ctgggtgaga aactcggttc ggaagccgac gagggcccgg gccgcgacga gatactccat
     2761 ccgaacccac ccggtgccat ggttgaccat ctgctccagt cggcccttgc ggcttgcaag
     2821 gagttgggtc acgacaccga ggtgctcctc gggaacgtcg atcgcgaccc gctc
//
LOCUS       DATCCH010000047         2297 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_295857, whole genome shotgun sequence.
ACCESSION   DATCCH010000047 DATCCH010000000
VERSION     DATCCH010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295857"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="VIJ44_01010"
     CDS             complement(<1..717)
                     /locus_tag="VIJ44_01010"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEY5164506.1"
                     /translation="MSLSPALRPLRLPRLSPTAYRRITLVAAVLLAVIIVTGGAVRLT
                     GSGLGCPAWPNCDPGHLTPRSPSDVNAMVEFLNRVFTGSVSIAVGLAVIGALLRLPRR
                     RDLIWLSLGLVAGFFAQAVLGGLTVLFQLQPQFVMAHFLLSLVLLANAIVLHHRAGRA
                     GGPSPLRVAPVVRRVATALLVAVSIVAVTGTVVTASGPHGGDAKAKRFSYSIPQVARV
                     HGTMVMVFLALVLLTFGLLRR"
     gene            839..1750
                     /locus_tag="VIJ44_01015"
     CDS             839..1750
                     /locus_tag="VIJ44_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019929953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY5164507.1"
                     /translation="MADPASYDEFGLFRENAEEYGLAWNGPPTVRRVVVPVASDGRKL
                     SALVWGDRPPEIVLLHGGAQNAHTWDTMALALDRPLVAVDLPGHGHSDHRDDGPFGPR
                     QNAGDVAVALRKLAPDAPMVVGMSLGGLTAIALTAEAPELVRRLALVDVTPGVDRDKA
                     APIAQFIAGPATFETFDEILARTVEFNPTRSVSSLRRGVLHNAVERDDGRWVWRYQRP
                     RLMEATAMDVPADFATLWDDVSGIKVPLMLVRGGATGTVVDDGDVAELLRRQPDARVE
                     LVEGAGHSIQGDRPVELAALLDSFLSS"
     gene            complement(1787..2206)
                     /locus_tag="VIJ44_01020"
     CDS             complement(1787..2206)
                     /locus_tag="VIJ44_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY5164508.1"
                     /translation="MSRHIEEFLADNRSGWTRVTPTHASTEVARGALLVDIRPLEQRR
                     RQGDVPGAIVVGRTVLEWRLAPFSDHRIPQAAPDARVIVLCGQGYSSSLAAASLRALG
                     LDATDVVGGFESWAAAGLSVESCRGPSREEATGFDTF"
BASE COUNT          363 a          699 c          926 g          309 t
ORIGIN      
        1 tcgtcggagc agcccgaagg tcaggaggac gagggcgagg aacaccatga ccatggtccc
       61 gtgtacgcgg gcaacttggg ggatgctgta cgagaagcgc ttcgctttcg cgtcgccgcc
      121 gtgcgggccg ctggcggtca caaccgtgcc ggtgacggcg acgatgctca ccgcgacgag
      181 cagggcggtc gcgactcgcc ggacaacggg cgcgacccgc agtggcgagg gtcccccggc
      241 gcgaccggcg cggtgatgca agacgatcgc gttcgcgagc agcacgagag agaggaggaa
      301 gtgggccatc acgaactggg gctgcagctg gaacagcacg gtgaggccac cgaggactgc
      361 ttgcgcgaag aagccggcga cgagcccgag ggacagccag atgaggtctc ggcggcgtgg
      421 gaggcggagc agcgccccga tgaccgcgag gccgacggca atggagaccg agccggtgaa
      481 gacccggttg aggaactcga ccatggcgtt gacgtcagac gggctgcgag gggtgaggtg
      541 accggggtcg cagttcggcc acgcggggca gccaagcccg gagccggtga ggcgcaccgc
      601 gccgccggtg acgatgatga cggcgaggag gacggcggcg acgagcgtga tccgccggta
      661 ggcggtcggt gagagacgcg gcagcctcaa cggcctcaac gcgggagaca ggctcatcgg
      721 cggctcggat gctacgccgg cgcgccgcgg acaccatgat cggcaggtcg gaccggacgg
      781 cgggggttgg atgtgacccg gtcgaccggt gcgggtcgag ccgttagcgt ccccgcggat
      841 ggctgacccc gccagctacg acgagttcgg gctcttccgc gagaacgccg aggagtatgg
      901 cttggcgtgg aacgggccac cgacggtgcg tcgggtggtg gtgccggtcg cgtcagacgg
      961 acggaagctg agcgcgttgg tctggggtga ccgcccgccg gagatcgtgt tgctgcatgg
     1021 tggtgcgcag aatgcgcaca cgtgggacac gatggccctg gcgctcgacc ggccgttggt
     1081 cgcggtggat ctccccgggc acgggcactc cgatcatcgt gacgacgggc cgttcggtcc
     1141 tcgccagaac gccggtgacg tcgcggtcgc gctgcgcaag ctggcaccgg acgcgcccat
     1201 ggtggtcggc atgtcgttgg ggggcctgac cgcgatcgcg ttgaccgcgg aggcgccgga
     1261 gctggtccgc cgattggcgc tggtggacgt caccccgggc gtggatcggg acaaggcggc
     1321 accgatcgcg cagttcatcg ccggcccggc gacgttcgag accttcgatg agatcctcgc
     1381 ccggacggtc gagttcaacc cgacccggtc ggtgtcttcg ctgcgacgcg gtgtcctgca
     1441 caacgcggtc gaacgggacg acggacgatg ggtgtggcgc tatcagcgac cgcggctgat
     1501 ggaagcgacg gcgatggacg taccggcgga cttcgcgacg ttgtgggacg acgtgagcgg
     1561 catcaaggtc ccgctgatgc tggtgcgggg aggtgcgacc ggcacggtgg tcgacgacgg
     1621 tgatgtcgcg gagctgctgc gacggcaacc ggacgcgcgc gtcgagctgg tggagggcgc
     1681 cgggcacagc atccagggcg accgcccggt cgagctcgcc gcgctcctgg actcgttcct
     1741 cagttcgtag cgaccggcac caccggcgtc gggtggcggg cagggattag aaggtgtcga
     1801 agccggttgc ttcttcgcgc gacggtcctc ggcacgactc gacgctgagc ccggctgcgg
     1861 cccaggactc gaagccgccg accacgtcgg tggcgtcgag accgagcgcc cggagcgacg
     1921 ccgcggcgag gctcgacgag tagccctggc cgcagagcac gatgacgcgc gcgtcgggtg
     1981 cggcctgcgg gatccggtga tcgctgaagg gggcaagccg ccactcgagg acggtgcggc
     2041 cgacgacgat cgcgccgggc acgtcgcctt ggcggcgtcg ttgctcgagg ggccggatgt
     2101 cgaccaggag cgcgccgcgg gcgacctcgg tggaggcgtg ggtgggtgtg acccgagtcc
     2161 agccggaccg attgtcggcg aggaactctt cgatgtggcg ggacacgggc tgcacggtaa
     2221 cgatggacgg atggacgcgc gcggtgaagc gcaggctcgg ctggagaccc tcgagccggg
     2281 acgcttcgag gctcggg
//
LOCUS       DATCCH010000048         3098 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_44395, whole genome shotgun sequence.
ACCESSION   DATCCH010000048 DATCCH010000000
VERSION     DATCCH010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44395"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..745)
                     /gene="obgE"
                     /locus_tag="VIJ44_01025"
     CDS             complement(<1..745)
                     /gene="obgE"
                     /locus_tag="VIJ44_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEY5164509.1"
                     /translation="MPNSAFVDEVQVNLRGGDGGAGAVSFRRESHVPKGGPDGGDGGD
                     GGAVVLEADRNVASLLAFRDHPHRRATSGTHGAGKRRHGSRGSDLIVAVPEGTLVHTR
                     AGELLADLAQHGDRYVAAPGGSGGRGNARFLSNARRAPSFAQQGEYAEEHWLRLEVQL
                     AADAALVGFPNAGKSTLISSVSAARPKIADYPFTTLEPHLGVVRAAGREFVLADIPGL
                     IEGAADGRGLGHRFLRHVERARVLVLLLDL"
     gene            complement(825..1079)
                     /gene="rpmA"
                     /locus_tag="VIJ44_01030"
     CDS             complement(825..1079)
                     /gene="rpmA"
                     /locus_tag="VIJ44_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010524945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEY5164510.1"
                     /translation="MSKKKGAASSRNGRDSAAQRLGVKVGAGTNVRAGTIIVRQRGTR
                     FHPGDNVGRGGDDTLFATADGTVRFGHRRGRKLIDVVSAA"
     gene            complement(1093..1404)
                     /gene="rplU"
                     /locus_tag="VIJ44_01035"
     CDS             complement(1093..1404)
                     /gene="rplU"
                     /locus_tag="VIJ44_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016838408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEY5164511.1"
                     /translation="MYAVVRTGGKQYRVEPGQRLQVERLGVDTGTDVELAPVLFVDGD
                     RVLATPDQLGATRVVARVVEEGRGKKIRGSTYKSKSNQRRRWGHRQGYSLVEITKIAA
                     G"
     gene            complement(1431..>3098)
                     /locus_tag="VIJ44_01040"
     CDS             complement(1431..>3098)
                     /locus_tag="VIJ44_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEY5164512.1"
                     /translation="GPTPVIEGGLTDADAAAEARDDAGLTPDAEASPGNAKPKIGDRR
                     PAPPATETAPSTASPGSSSGRRRRRGGRGRSRTGAAAGSATTTTNTDAPTDDVVRADL
                     GLGDAVDLEDLDDAQRARRRGRTRKGRPAGRYQLLVHVRPNAHTHIALLEGRVLVEHS
                     VSTPTDADTSIDGNIYLGRVQNVLPGMEAAFIDIGTPKNGVLYRGDVSFDKDEVEEKQ
                     PRIERLLKPGQSIVVQVTKNPIGAKGARLTQEVSLAGRFVVMVPGQPETYGISKRLPD
                     DERRRLRGVLEGLRPPDAGLIVRTAAEGAADEELERDVRRLREQWEQISALAARSKPG
                     RVLYREPLLALRVIREEFTKEYRGVLIDDRDLYEEVRAYIEAVTPELAERVEYYDADA
                     EGLPIFERFHVNEQLLKALDRKVWLPSGGSLVIERTEALTVIDVNTGKNVGSSSLEET
                     VFRNNLEAAEEIARHLRLRDIGGIIVIDFIDMEIRNNREQVQQAFRNALGRDKTRTQA
                     FDISELGLVEMTRKRISEGLVESMSDTCPTCQGRGYLVEESLLEELQ"
BASE COUNT          385 a          979 c         1208 g          526 t
ORIGIN      
        1 ggaggtcgag gaggagcacg aggacccggg cccgttcgac gtggcgcagg aaacggtggc
       61 cgaggccccg cccgtcggcg gccccctcga tgaggcccgg gatgtcggcg aggacgaact
      121 cgcggccggc ggcgcggacc accccgaggt gcggttccag ggtggtgaac ggatagtcgg
      181 caatcttggg tcgggcggcg ctgaccgagg agatgagggt cgacttgccg gcgttcggga
      241 accccacgag ggccgcgtcg gcggccagct gcacttcgag tcgcagccag tgctcctcgg
      301 cgtattcgcc ttgctgggcg aagctcgggg cccgccgggc gttggagagg aaccgggcgt
      361 tgccgcgtcc gccgctcccg ccgggggcgg cgacgtaacg gtcgccgtgt tgcgccaagt
      421 cggcgaggag ctccccggcc cgcgtgtgca ccagggtccc ttccgggact gcgacgatca
      481 ggtcggagcc tcggctcccg tggcgtcgtt tccccgcgcc gtgggtgcca gaggtggctc
      541 gtcggtgcgg gtggtctcgg aaggcgagga gcgacgcgac gttgcggtcg gcttcgagca
      601 ctaccgcgcc gccgtcgccg ccgtcgccgc cgtcggggcc gcctttgggg acatgggatt
      661 cgcgtcggaa cgacacggct ccggcgccgc cgtcgccgcc gcgcaggttg acctgcacct
      721 cgtcgacgaa cgcggagttg ggcatgcctc gaggataggg agcagcgcgg cgcggtcggc
      781 gccgatactt gggcggtgga cggcggtgct ggcggcgcgg cgggtcaggc ggcggaaacg
      841 acgtcgatca gcttgcggcc tcggcggtgc ccgaaccgca cggtgccgtc ggcggtggcg
      901 aacagggtgt cgtcgcctcc gcgcccgacg ttgtcgccgg ggtggaaccg ggtgccgcgt
      961 tgacggacga tgatcgtccc ggcccggacg ttcgtgccgg cgccaacttt gacgccgaga
     1021 cgctgcgcgg cggagtcgcg gccgttgcgg gaggacgcgg cacctttctt cttcgacatg
     1081 gggtgtcctt gctcagccgg cggcgatctt ggtgatctcg accagggagt acccctggcg
     1141 gtgaccccag cgtcgacgct ggttggattt cgacttgtag gtggagcctc ggatcttctt
     1201 gcctcggcct tcttcgacga cacgggccac gacccgggtg gcgccgagct ggtcgggggt
     1261 ggcgaggacc cggtcgccgt cgacgaacag gaccggggcc aactcgacgt cggtgccggt
     1321 gtcgactccg agccgctcga cttgcagtcg ctggccgggc tcgacccgat attgcttgcc
     1381 gccggtgcgg accaccgcgt acatcagacc tccgagggga ccggggaagc ctactgcagc
     1441 tcttcgagga ggctctcctc gacgaggtag ccgcggcctt ggcaggtggg gcaggtatcg
     1501 ctcatcgatt cgacgaggcc ttcggagatg cgtttgcggg tcatctcgac cagaccgagc
     1561 tcggagatgt cgaacgcctg ggtgcgggtc ttgtcgcggc cgagcgcgtt gcggaacgcc
     1621 tgctggacct gctcccggtt gttgcggatc tccatgtcga tgaagtcgat gacgatgatt
     1681 ccgccgatgt cgcggagacg cagatgtcgg gcgatttcct ccgcggcttc gaggttattg
     1741 cggaacacgg tctcttcgag gctcgaggag ccgacgttct tgccggtgtt gacgtcgatg
     1801 acggtgaggg cctcggtgcg ctcgatgacg agcgacccgc ccgacgggag ccacaccttg
     1861 cggtcgaggg ctttgaggag ctgctcgttg acgtggaacc gctcgaagat cggtagtcct
     1921 tcggcgtcgg cgtcgtagta ctcgacccgt tcggcgagct cgggggtcac cgcctcgatg
     1981 taggcgcgga cttcctcgta gagatcgcgg tcgtcgatga ggacgccgcg gtactccttg
     2041 gtgaactcct cgcggatgac ccgtagtgcc aggagcggct cccggtacag gacccggccg
     2101 ggcttcgagc gggcggcgag ggccgagatc tgctcccact gctcgcggag gcgccgcacg
     2161 tcgcgttcga gctcctcgtc cgcggcgcct tcggcggcgg tgcggacgat gagtccagcg
     2221 tcgggtgggc gaagcccttc gaggacgccg cgcagacggc ggcgctcgtc gtcgggaagg
     2281 cgtttggaga tgccgtaggt ctcgggctgg cccgggacca tcaccacgaa ccgtccggcg
     2341 aggctgacct cttgggtgag gcgggcccct ttggcgccga tcgggttctt ggtgacctgc
     2401 accacgatgc tctggcccgg tttgaggagc cgctcgatgc ggggctgttt ctcctccacc
     2461 tcgtctttgt cgaaggacac gtcgccgcgg tagagaaccc cgttcttggg ggtgccgatg
     2521 tcgatgaacg cggcttccat gccgggcagc acgttctgga cccggcccag gtagatgttg
     2581 ccgtcgatcg aggtgtcggc gtcggtgggg gtgctcaccg agtgctcgac gaggacgcgt
     2641 ccttcgagga gcgcgatgtg ggtatgggcg ttcgggcgga catggaccag gagctggtag
     2701 cgcccggcgg gacgaccctt gcgggtccgg ccccggcggc gggcccgctg ggcgtcgtcg
     2761 aggtcttcga ggtcgacggc atccccgagg ccgaggtcgg cgcgcaccac gtcgtcggta
     2821 ggtgcgtcgg tgttggtggt cgtggtggca ctgccggcgg cggcaccggt gcggctgcgg
     2881 ccccgcccac cgcgccggcg gcgccgtccc gacgaggagc cgggcgaggc ggtgctcggc
     2941 gccgtctcgg tggcgggggg cgcggggcgg cggtcgccga tcttgggttt ggcgttcccg
     3001 ggagacgctt ccgcgtcggg ggtcagcccg gcgtcgtcgc gggcctcagc ggccgcgtca
     3061 gcgtcggtga ggccaccctc gatgacgggg gtcgggcc
//
LOCUS       DATCCH010000049         2546 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_544933, whole genome shotgun sequence.
ACCESSION   DATCCH010000049 DATCCH010000000
VERSION     DATCCH010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544933"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1400)
                     /locus_tag="VIJ44_01045"
     CDS             complement(<1..1400)
                     /locus_tag="VIJ44_01045"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEY5164513.1"
                     /translation="MPSKILIANRGEIALRVMRTCRELGVATVAVYSDLDRDAAHVRY
                     ADEAYALGGQTAAESYLNTERILQAIERSGADAVHPGYGFFAENADFARAIGTAGVTW
                     VGPPPEAIEVMGDKISSRKAANAAGVEAVPGTIEAITDVDEIVAFGEQHGWPVAIKAA
                     YGGGGKGLKVVRGADGAQAAFESATREATAYFGRPEAYLERYLTRPRHIEMQVFCDTH
                     GHGVWLGERDCSTQRRHQKLIEETPAAGLDDATRSAMGAAAVKVALGCGYVNAGTVEM
                     LYQDGAFWFLEMNTRLQVEHCVTEMVTGLDLVAEQLRVADGEALSFTQESVQRDGHSI
                     ECRINAEDPSRGFLPSPGTITRLRVPGGPGVRWDAGYEEGDTISQYYDNLVGKLVVWG
                     PDRDGARHRMLRALGEIDIEGIHTTTPAHRVLIAHPDFAAGHHSTKWVEEELDEAIFA
                     TAPTPAVESPPADDGPA"
     gene            1454..2233
                     /locus_tag="VIJ44_01050"
     CDS             1454..2233
                     /locus_tag="VIJ44_01050"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEY5164514.1"
                     /translation="MGDAEASPGVRWRLRRFDEIDSTNRYALDAARAGTAAGLVVVAD
                     HQQAGRGRRGRAWTAPPGSSLLVSVLLRPRLAADQVHVVTMAAALALVDAVRLVAGVD
                     ADLKWPNDLLVDERKLAGLLAEADITGGVVRAVVIGVGCNVTWGGFPSELEDIATACD
                     REAGRPVERTALLDAFLARLGARIETLDRVPGDYRSRLATIGRRVRVELTDRTLEGVA
                     TGVDDHGRLVVQPGTGPSVTVAAGDVVHLRGVTDPGSPRST"
     gene            complement(2202..>2546)
                     /locus_tag="VIJ44_01055"
     CDS             complement(2202..>2546)
                     /locus_tag="VIJ44_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019360030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEY5164515.1"
                     /translation="FANTRGELCEATGSNVFVVIDNLVVTPPETSGCLLGVTRALVLE
                     LCEDLGVASDERALPLAALADADEAFLTSSTREVQPIARVDGQALPAAPGPLSSRLAD
                     GFVTLIAANLDP"
BASE COUNT          353 a          890 c          909 g          394 t
ORIGIN      
        1 gccggaccgt catcagcggg aggcgactcg acggcggggg tcggcgcggt ggcgaagatc
       61 gcctcgtcga gctcctcttc cacccacttc gtcgagtggt gaccggcggc gaagtcgggg
      121 tgcgcgatca ggacgcggtg cgccggcgtc gtggtatgga tcccctcgat gtcgatctcg
      181 cccagggcgc ggagcatgcg atggcgggcc ccgtcccggt cggggcccca caccacgagc
      241 ttgccgacga ggttgtcgta gtactgggag atggtgtcgc cctcttcgta gccggcgtcc
      301 caccgcaccc ccggtccacc cgggacccga aggcgagtga tcgtccccgg tgagggaaga
      361 aacccgcggg acggatcctc ggcgttgatg cggcactcga tcgagtggcc atcccgttga
      421 accgactctt gggtgaagga caaggcctcg ccgtcggcga cgcggagctg ctcggcgacg
      481 aggtcgagac ccgtcaccat ctcggtcacg cagtgctcga cctgcaagcg ggtgttcatc
      541 tccaagaacc agaaggcgcc gtcctggtag agcatctcga cggtgccggc gttgacatac
      601 ccgcagccga gcgcgacttt caccgcggcg gcgcccatgg ccgagcgggt cgcgtcgtcg
      661 agaccggccg cgggggtctc ctcgatcagc ttctggtggc ggcgctgcgt cgagcagtcc
      721 cgttcgccga gccagacgcc gtggccgtgg gtgtcgcaga agacctgcat ctcgatgtgt
      781 cgaggccggg tgaggtaccg ctcgaggtac gcctcgggcc gcccgaaata cgccgtggcc
      841 tctcgggtgg cggactcgaa cgctgcctgg gcgccatcgg cgccgcggac caccttcagg
      901 cctttcccgc cacccccgta cgcagccttg atcgccaccg gccagccgtg ctgctcaccg
      961 aaggcgacga tctcgtcgac gtcggtgatc gcctcgatcg tccccgggac cgcctcgaca
     1021 cccgccgcgt tcgcggcctt gcgggacgag atcttgtcgc ccatcacttc gatggcctcg
     1081 ggaggcgggc cgacccacgt gacccccgcg gttccgatgg cgcgcgcgaa gtcggcgttc
     1141 tcggcgaaga agccgtaccc ggggtggacg gcgtcggcac cgctgcgctc gatggcttgg
     1201 aggatccgct cggtgttcag gtagctctcg gccgcggtct gaccgcccaa cgcgtacgcc
     1261 tcgtcggcgt agcgaacgtg cgccgcgtcg cggtccaggt cggagtacac cgcgacggtc
     1321 gcgaccccga gctcccggca ggttcgcatc acgcgcagcg cgatctcgcc acgattcgcg
     1381 atgaggatct tggacggcaa cgggcccccg gtctcgtgag gtcgtgggga aaggtcgccg
     1441 tatcctacgg gtgatgggtg atgcggaggc cagcccgggt gttcgctggc gcctccgtcg
     1501 attcgacgag atcgactcca cgaaccggta cgcgctcgac gcggcccgcg ccggcaccgc
     1561 cgccgggctc gtcgtcgtgg ccgatcatca acaggccggg cgagggcgtc gggggcgcgc
     1621 ctggaccgcg cctcccgggt cgtcgctcct cgtctcggtg ctgctccgcc cgcgtctcgc
     1681 ggctgatcag gtgcacgtcg tgacgatggc tgcggccctc gcgcttgtcg acgcggtccg
     1741 actggttgcg ggagtcgacg ccgacctcaa gtggcccaac gacctcctcg tcgacgagcg
     1801 caagctcgcc gggttgctcg ccgaagctga catcaccggt ggtgtcgtgc gggcagtcgt
     1861 gatcggagtg ggctgcaacg tcacctgggg agggttcccc tccgagctgg aggacatcgc
     1921 caccgcgtgc gaccgagagg cgggacggcc cgtcgagcgc accgcgctcc tcgacgcgtt
     1981 cctggcccgg ctcggcgcgc gaatcgagac cctcgaccgc gtgcccggcg actatcggtc
     2041 ccggctcgcc acgatcggac gtcgggtccg cgtcgagctg accgaccgga cgctcgaagg
     2101 cgtcgcgacc ggagtcgatg accacggccg tctcgtcgtc caaccgggca ccggcccgtc
     2161 ggtgaccgtc gcggccggtg acgtcgtgca tctgcgcggc gtcacggatc caggttcgcc
     2221 gcgatcaacg tgacgaaccc gtcggcgagc cgcgacgaaa ggggcccggg cgccgccggc
     2281 agcgcctgac cgtcgacgcg cgcgatgggc tgcacctctc gggtgctcga ggtcaggaac
     2341 gcctcgtcgg cgtcggcgag cgcggcgagg ggcagcgccc gttcgtcgct cgccaccccg
     2401 aggtcctcgc acagctcgag gaccaaggcg cgggtcacgc cgagcaggca gcctgacgtc
     2461 tcgggtggtg tcacgacgag gttgtcgatc accacgaaga cgttcgaacc ggtggcttcg
     2521 cagagctccc cgcgggtgtt ggcgaa
//
LOCUS       DATCCH010000050         2215 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_545079, whole genome shotgun sequence.
ACCESSION   DATCCH010000050 DATCCH010000000
VERSION     DATCCH010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545079"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VIJ44_01060"
     CDS             <1..215
                     /locus_tag="VIJ44_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164516.1"
                     /translation="TVTRNIQSGVVTTMTRTFQPVGHTTRVTATVEWEIPVRSVARLI
                     AAPLRGPYRRGLRHSLRAADQSLTTG"
     gene            complement(222..1562)
                     /locus_tag="VIJ44_01065"
     CDS             complement(222..1562)
                     /locus_tag="VIJ44_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY5164517.1"
                     /translation="MTGTAADTTAETTDLLQILIRNACVNDGHVESGEEARSVEALEA
                     YLGTAGLDVERYEPQPGRTSLVTRIEGSDPDAPSLLLMGHTDVVPVNPDGWSREPFSG
                     ELVDGFVWGRGAVDMLNITASQAVAMRRLAQSGFTPRGTLTYLAVADEEALGTWGAQW
                     LVEHERDAVYADYVLTESGGYQMATPEGTRLPVLTAEKGTFWSKLRIRGTPGHGSQPF
                     RTDNALVTAAEVVRRLSEFQPEAHMHDVWRRFVEGMHYPPELHDALLDPVELPNVLGE
                     LPLGMSRLVHACTHTTFAPTVAHGGTKTNVIPDLVELEVDIRTLPGQTGTDALDLVRE
                     ALGDLADQVEIESNDDPSTASPIETPLWDSLARVTGRLVDGSALVPFLMVGGTDNRFF
                     RRAGSVGYGFALFSQRLSFEDYATMFHGNDERVDVESLGLSEQLWEAVAHDLLD"
     gene            complement(1619..>2215)
                     /locus_tag="VIJ44_01070"
     CDS             complement(1619..>2215)
                     /locus_tag="VIJ44_01070"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY5164518.1"
                     /translation="AGPPLRRRLMIRALRFLFSPPAPTRRVVHGQRLQLAGREFVSIH
                     TPGHTIDHLCLYDPENGVLLSGDHVLPTITPHVSGVGNGADALRSYLQTLDLVAALDG
                     VTLGLPAHGHPFDDVPGRVDAIKRHHEERMQLLLDASAQIGPATVEELAHEIFPRKHW
                     GVMAESETFAHLEHMVLAGSAERWREHGRLLYRGAPAA"
BASE COUNT          325 a          739 c          816 g          335 t
ORIGIN      
        1 gcacggtgac ccgcaacatc cagtccgggg tggtcacgac catgacccgg acgttccagc
       61 cggtcggcca caccacgcgg gtcaccgcca ccgtggaatg ggagatcccg gtgcgttccg
      121 tggcgcggct catcgcggcg ccgctacgcg gaccgtaccg gcggggcttg cgccactccc
      181 tccgcgccgc cgaccagtca ctgacgaccg gttgaccgac ctcagtcgag gaggtcgtgc
      241 gcgacggctt cccagagctg ctccgagagc ccgagcgact cgacatcgac gcgctcgtcg
      301 ttgccgtgga acatcgtggc gtagtcctcg aaggacaggc gctgggagaa cagcgcgaac
      361 ccgtagccga ccgatccggc ccggcgaaag aaccggttgt cggtcccgcc gaccatgagg
      421 aacggcacca gcgccgaccc gtcgacgaga cggcccgtga cccgggccag cgagtcccac
      481 agcggcgtct cgatcggcga cgcggtcgac gggtcgtcgt tcgactcgat ctcaacctgg
      541 tcggcgaggt caccgagcgc ttcgcgcacc agatccaggg cgtcggtacc ggtctgaccg
      601 ggaagagtgc gaatgtcgac ctcgagctcg acgaggtcgg ggatgacgtt cgtcttcgtc
      661 ccgccgtgcg cgaccgtcgg cgcgaacgtg gtgtgggtgc acgcgtgcac caggcgcgac
      721 atcccgagcg gcagctcgcc gaggacgttg ggcagctcaa caggatcgag gagcgcgtcg
      781 tgcagctcgg gtgggtagtg catgccttcg acgaagcgtc gccacacgtc gtgcatgtgg
      841 gcctcgggct ggaactcgct gaggcgacgc accacctcgg cggcggtcac gagggcgttg
      901 tcggtccgga acggctggga cccgtggccc ggggtgcccc ggatccgcag cttggaccag
      961 aaggtgcctt tctcggcggt gaggacgggg agccgggtcc cttccggggt cgccatctgg
     1021 tatccgccgg attcggtcag gacgtagtcg gcgtacaccg cgtcgcgctc gtgctcgacg
     1081 agccattgcg cgccccacgt gccgagcgcc tcttcgtctg cgaccgcgag gtaggtgagc
     1141 gtccctcgcg gggtgaagcc cgactgggcg aggcggcgca tcgccaccgc ctgcgacgcg
     1201 gtgatgttga gcatgtcgac tgcgccgcgt ccccacacga agccgtcgac gagttcaccc
     1261 gagaacggct cccggctcca cccgtcgggg ttgaccggta cgacgtcggt gtggcccatg
     1321 agcagcaggc tcggcgcgtc ggggtcgctg ccctcgatgc gggtgacgag gctggttcgc
     1381 ccgggttggg gttcgtaccg ctcgacgtcg agcccggcgg tgccgaggta cgcctcgagg
     1441 gcttcgacgc tgcgggcctc ttcgcctgat tcgacgtggc cgtcgttgac gcaggcgttg
     1501 cggatgagga tctgaagcag gtcggtggtc tcggcggtgg tgtccgccgc ggttccggtc
     1561 acagcggctc agggtaccgg ggtccggagc agtcggcgcc ggccacgcgc cgcgaggctc
     1621 aggccgcggg cgcgccgcgg tacaggagcc gaccgtgttc gcgccaccgc tcggcgctgc
     1681 ctgcgagcac catgtgctcc aggtgggcga acgtctcact ctcggccatg accccccaat
     1741 gcttgcgggg gaagatctcg tgcgcgagct cctcgaccgt cgccggcccg atctgagctg
     1801 acgcgtcgag caacagctgc atgcgctcct cgtggtgacg cttgatggcg tcgacccgac
     1861 cggggacgtc atcgaacgga tgcccgtgcg ccggaagccc gagggtgacg ccgtcgaggg
     1921 cggcgacgag gtccagggtc tgcaggtagg accggagcgc gtcggcgccg ttcccgaccc
     1981 ccgacacgtg gggcgtgatc gtcggcagca cgtgatcacc ggagaggagg acgccgttct
     2041 cagggtcgta gaggcacagg tggtcgatgg tgtgccccgg ggtgtggatc gacacgaact
     2101 ctcgtcccgc cagctggagt cgctggccgt gcaccacacg acgggtcggt gccggcggtg
     2161 agaagaggaa ccgcagcgcg cggatcatca gccggcgtcg aagcggcggc ccggc
//
LOCUS       DATCCH010000051         2945 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_254709, whole genome shotgun sequence.
ACCESSION   DATCCH010000051 DATCCH010000000
VERSION     DATCCH010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254709"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="VIJ44_01075"
     CDS             <1..424
                     /locus_tag="VIJ44_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY5164519.1"
                     /translation="FFAEGDSPGLEDAYRSAAAEAGLAPGTCIVPTRGNPTTLFVAED
                     LDTAWDRIGPHMLHDAQMYGSWMGADHDAASKSSAVTVEELRAEQGPYRIVTPPTAVE
                     MIQAGQPLGLQPLCGGLNPEVAWESLNLLSERVLPALG"
     gene            complement(549..1067)
                     /locus_tag="VIJ44_01080"
                     /pseudo
     CDS             complement(549..1067)
                     /locus_tag="VIJ44_01080"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin-like family protein"
     gene            complement(1097..2263)
                     /locus_tag="VIJ44_01085"
     CDS             complement(1097..2263)
                     /locus_tag="VIJ44_01085"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5164520.1"
                     /translation="MSDARVVDADCHILEPPDIWKNWLPEQYQEKAPRLVKDPAGGDA
                     WLTAVGGAADPIGLVSTPGMPWDQFRWFGVTYDEARPGCYNGAARLKDMDIDGVWAEV
                     LYPPQRTMSHFLGDDDDDFVLAGVDAYNNFLFEEFCAPDPTRLIGLAQMPSLGIDQAI
                     DALRKAKARGAKGVVISNWPSGQEGLSSDDDPFWAAAVDEGIPVAVHINIISRKQRAA
                     SRKAAAKRGNALYDMSSEATRAKAIGSMSHVFSMCAGSITQMIFTGVFERFPELQMCW
                     IETGVGWIPHLIESIDDRYWRNRVWGDLPIKEPPSSYWYRNNAATFITDRSGIALRHA
                     AGIDNIMWSSDYPHHGNDWPYSRKMIEDTMGQIPNDEKVKITGANAARIWHLDR"
     gene            2400..>2945
                     /locus_tag="VIJ44_01090"
     CDS             2400..>2945
                     /locus_tag="VIJ44_01090"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY5164521.1"
                     /translation="MATALLDDIRVVDLAGEPAALTGRILADLGAEVVLVEPPGGHPL
                     RSDPDRFAAWSAGKTSLAVRHADDPALLELLAAADIVVDTPGFPGTLDVDAGRAPQAV
                     WVSVTPFGRSGPRSGWVASDLGVMAASANMYCTGDPDRPPVRCTEPSGYAHTGPEAAF
                     AALTALWTGRPQHVDVSMQEAV"
BASE COUNT          460 a         1012 c          988 g          485 t
ORIGIN      
        1 cttcttcgct gaaggagaca gccccggtct cgaagacgcc taccggagtg ccgcggccga
       61 ggccggcctc gcgcccggga cctgcatcgt cccgaccaga ggtaacccga cgacgctgtt
      121 tgtcgccgag gatctcgaca cggcttggga tcggattggt cctcacatgt tgcacgacgc
      181 tcagatgtac ggatcgtgga tgggcgccga ccacgacgcg gccagcaagt cgtcggcggt
      241 gaccgtcgag gagctccgag ccgagcaagg cccctaccga atcgtgacgc cgccgaccgc
      301 agtcgagatg atccaagcgg gccagccact tgggttacag ccgctctgcg gcgggctgaa
      361 ccccgaagtg gcctgggaga gcctgaacct cctcagtgag cgagtcctcc cggcgttggg
      421 atagccacga gctacagcca tcccagcttg cgccaccaac tcggggctcg gtctcgggag
      481 tgggcgctcc ggtggaggcg cgacgcaagc cggcccgatt gccgtgccgt gctcccgtgc
      541 tcccgtgctc aacgcgccgc gcgcacggcg gcgaccaact cgtcgacgga tggtcggtcg
      601 tcaggtgtgc gggaccgata cccgtaggca acccgaccgc cggaatcaac gacgaagtct
      661 ccaccgagct gccgcgtgtc ctcggtggga cgacgaagcc gatggccggt gcgcagcagc
      721 cgcccgtacg tgcggagcgt gccccagctc cagacacgcc gccaccgccc ccgctccaga
      781 ccgtacgcgc ggtagaccgc gcgcgtttcg tcgaccagca ccggatagtt gagctcgagt
      841 agacgccgga agccacggag gttgcgttgg cgcgtgaaag tcaccagcac gaccgatgcc
      901 ttctcgaact catcgaggcg atcgctcacg gcgaccgcgt gctcatggca cggaagtcat
      961 gccagatgtc gatggaagat caagaccacc ggttggcctc gctgctccga gagccgccag
     1021 ccggcgtctt gctcgtcggg cagggcgaag tcgggtgcgg tatcgcctgt ctgcaccgcc
     1081 atgccagttc ctcttgctag cggtcgaggt gccagatccg cgccgcgttg gccccggtga
     1141 tcttgacctt ctcgtcgttt gggatctgac ccatcgtgtc ttcaatcatc ttgcgtgagt
     1201 atggccagtc gttgccgtga tgcgggtaat cgctcgacca catgatgttg tcgatccccg
     1261 ccgcgtggcg cagcgcgatc cccgaccggt cggtgatgaa cgtcgccgcg ttgttgcggt
     1321 accagtacga cgacggcggc tccttgatcg ggaggtcacc ccagacccgg tttcgccagt
     1381 agcggtcatc gatcgattcg atgagatgcg ggatccagcc gacaccggtt tcgatccagc
     1441 acatctgcag ctccgggaac cgctcgaaca cgccggtgaa gatcatctgg gtgatgctcc
     1501 cggcgcacat cgagaagacg tggctcatgc tcccgatcgc cttcgcccgg gtcgcctcgc
     1561 tgctcatgtc gtagagcgcg tttccgcgct tcgcggcggc tttgcgagac gcggctcgtt
     1621 gtttgcggct gatgatgttg atgtggaccg cgaccgggat cccctcgtcg accgcggcgg
     1681 cccagaacgg gtcgtcgtcg ctggagaggc cctcttggcc tgaaggccag ttcgagatca
     1741 cgaccccctt cgcgccgcga gcctttgcct tgcgcaacgc gtcgatggcc tggtcgatcc
     1801 cgagcgacgg catctgcgcc aagccgatga gacgggtggg atcaggagcg cagaactctt
     1861 cgaacaggaa gttgttgtag gcgtcgacgc cggccagcac gaagtcgtcg tcgtcgtcgc
     1921 cgaggaagtg gctcatggtg cgctgggggg gatagagcac ctcggcccag actccgtcga
     1981 tgtccatgtc cttgaggcgc gccgcgccgt tgtaacaacc ggggcgggct tcgtcgtagg
     2041 tgacaccgaa ccagcggaac tggtcccagg gcatcccggg tgtggacacc agcccgatcg
     2101 ggtccgcggc accgccgacc gcggtgagcc aggcgtcgcc gccggccggg tccttcacca
     2161 ggcgaggtgc cttctcctgg tactgctccg gcagccagtt cttccagatg tcgggcggct
     2221 cgaggatgtg acagtcggcg tcgacgacgc gggcatcaga catcggtgtc cctccaagct
     2281 cgaccggcgc gcctgcacag taggctgacg cgcgcgtcag gaaaagtctg gccgcaaccg
     2341 tctggggtac ggtccggtcg cgacgtcccg ggttggctga gggggaaggc gacaccgaca
     2401 tggcgaccgc cctcctcgac gacatccgcg tcgtcgacct ggccggcgag cccgccgccc
     2461 tgaccggccg gatcctcgcc gacctcgggg ccgaggtggt cctggtcgaa ccgcccggcg
     2521 gtcacccgct ccgctcggat cccgaccgtt tcgcggcgtg gagcgccggc aagaccagcc
     2581 tggcggtgcg acacgccgac gacccggctc tcctcgagtt gctcgccgcg gctgacatcg
     2641 tcgtcgacac gccgggcttc ccggggacgc tcgacgtcga cgccgggcgg gccccccagg
     2701 cggtctgggt cagcgtcacc ccgttcgggc gatcgggacc ccgttccggt tgggtcgcca
     2761 gcgacctcgg cgtcatggcg gccagcgcca acatgtactg caccggcgac cccgatcgac
     2821 ccccggtgcg ctgcaccgaa ccatccggat acgcgcacac cggacccgag gccgcgttcg
     2881 ccgcgctgac cgcgctctgg accggccgcc cgcagcacgt cgacgtgtcg atgcaagagg
     2941 cggtg
//
LOCUS       DATCCH010000052         2974 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_462713, whole genome shotgun sequence.
ACCESSION   DATCCH010000052 DATCCH010000000
VERSION     DATCCH010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462713"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            235..744
                     /locus_tag="VIJ44_01095"
     CDS             235..744
                     /locus_tag="VIJ44_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006238273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061136 - regulation of proteasomal protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome activator"
                     /protein_id="HEY5164522.1"
                     /translation="MTNPTPDGAAERTPVQPEVMDPAPPPPDRPVEDGEQVTSPTKLI
                     RIATMVRTMLDEVRRAPLDDAGRRRLREIHEKSLSELKGVLSPELQQELSDVVLPLTS
                     EAPTESELRLAQAQLVGWLEGLFHGIQATLFTQQAATQSQLDEMRRRGALEAAQGFGS
                     SETPGSGYL"
     gene            749..2110
                     /locus_tag="VIJ44_01100"
     CDS             749..2110
                     /locus_tag="VIJ44_01100"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HEY5164523.1"
                     /translation="MSTAEMFANDWSPDSWRRRSAEQQPDWPDPDALKDVEGELRGLP
                     PLVFAGEARTLTESLAAAQDGRAFLLIAGDCAESFQAFSADAIRDKLRVILQMSVVLT
                     YGSGVPTIKVGRMAGQFTKPRSSATEARGEVELPSFRGDTINDFTFDEFARRPDPSRL
                     LRAYHQSAATLNLVRAFAKGGFADLARVHAWNQEFVAASPEGRRYDVLAGEIERALRF
                     MEACGIDLETELALHQVDIWTTHEALLLEYEEALTRRDSITGDWYDCSAHLLWVGERT
                     RQPSGAHVEFLAGVHNPIGVKLGPSTTPDEVIALCDRLNPRRQPGRLVLFSRMGADRV
                     AEVLPPLLRVVRLSGHPVLWACDPMHGNTFVHSSGYKTRRFDDVLRELRGFFGACEAA
                     DVAAGGVHVELTGENVTECLGGSEEVLDEHLEQRYETVCDPRLNARQSLDLAFNVAGL
                     LRG"
     gene            2157..>2974
                     /locus_tag="VIJ44_01105"
     CDS             2157..>2974
                     /locus_tag="VIJ44_01105"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEY5164524.1"
                     /translation="MSFADGGVNQGLLDDLYARYRDDPSSVPERWRRFFDPARGNGAD
                     PAPPSTPPAAEATVVPAPPSPPAPGGAPIDAPSAAAVAPVEEAEVLRGAAARTVVNME
                     ASLGVPTATSVRTVPAKLLEVNRQILNNHLGRTGRGKVSFTHLIAFAVVRAQRDFPGL
                     NASYDEVDGQPTITRHAHVNLGLAVDVTRKDGTRTLLVPNIKTADQLDFAQFWSRYED
                     LIAKVRAGKLSPDDFTATTATITNPGMIGTVHSVPRLMAGQGFIVGVGSIGYPA"
BASE COUNT          464 a         1119 c          978 g          413 t
ORIGIN      
        1 atcgcttccg cgacgtcccc ggcccccgga ccgagagcgc cacggggcgg gtgccccccg
       61 gagtcgggga tcggggcggc attttcgagc cccggccctc gggctgaacc tgccgaggct
      121 cccggcgggc catgcgcccg cccgtcagct ccgccaaaca atcgcgggct tgcccagccc
      181 ggtccgtatc ctgggaccgt ctcctggccg gcgtcacaac ccacggagtg tctcgtgacc
      241 aaccccaccc ccgacggtgc cgctgagcgc acaccggtcc agcccgaggt catggatccc
      301 gcgccgccac cgcccgaccg accggtcgag gacggcgagc aggtcacgag ccccacgaag
      361 ctgatccgca tcgcgacgat ggtccgcacc atgctcgacg aggtgcgccg cgccccgctc
      421 gatgacgccg ggcgccgacg cctgcgcgag atccacgaga agtcgctgtc cgagctcaaa
      481 ggggtgctgt caccggagct gcaacaggag ctttccgacg tcgtgctgcc cctcacgtcc
      541 gaggcgccca ccgaatccga gctgcgtctg gcccaggccc aactcgtcgg atggctcgag
      601 ggcctgttcc acggcatcca ggccaccctc ttcacccagc aggccgcgac ccagtcccag
      661 ctcgacgaga tgcgacgacg cggggccttg gaagccgccc agggattcgg ctcgtccgag
      721 acccccggca gcggctacct ctagatcgat gtccaccgcg gagatgttcg cgaacgactg
      781 gtcgccggat tcgtggcggc gacggtccgc ggaacagcaa cccgattggc ccgaccccga
      841 cgcgctgaag gacgtcgaag gggagctgcg cgggctcccg ccgctggtgt tcgccggcga
      901 ggcacgcacg ctgaccgaat cgctcgccgc ggcccaggac ggccgagcgt tccttctcat
      961 cgcaggtgac tgcgccgaat cgttccaggc cttctcggcc gacgccatcc gcgacaagct
     1021 gcgcgtgatc ctgcagatgt cagtggtgct gacctacggg agcggcgtcc cgaccatcaa
     1081 ggtggggcgg atggcgggcc agttcacgaa gccgcgctcc tccgccactg aggcccgggg
     1141 tgaggttgag ctgccgtcat tccggggcga caccatcaac gacttcacct tcgacgagtt
     1201 cgcccgtcgt cccgacccga gccgtctgct gcgcgcgtac caccagtcgg cggcgacgct
     1261 gaacctggtc cgagccttcg cgaagggtgg cttcgcggac ctggcacgcg tgcacgcctg
     1321 gaaccaggag ttcgtcgccg ccagccccga ggggcgtcgc tacgacgtgc tcgcgggaga
     1381 aatcgagcgg gccttgcgct tcatggaggc atgcggcatc gacctcgaga cggagctggc
     1441 cttgcaccag gtcgacatct ggaccaccca cgaggcgctc ctccttgagt acgaggaggc
     1501 gctgacccga cgcgacagca tcaccgggga ttggtacgac tgctccgccc atctgctctg
     1561 ggtcggggaa cgaacccgtc agcccagcgg cgcccacgtc gagttcctcg ccggcgtgca
     1621 caacccgatc ggggtgaagc tcggaccatc aaccacgccc gacgaggtca tcgcgctctg
     1681 tgatcgcctc aacccgcgac gccaacccgg acggctggtg ctcttctcgc ggatgggcgc
     1741 ggaccgggtc gcggaagtcc tcccgccgct gctgcgggtc gtgaggctga gcggccaccc
     1801 ggtgctctgg gcgtgcgacc cgatgcacgg caacacgttc gtgcacagca gcggctacaa
     1861 gacgcggcgc ttcgacgatg tgctccgcga gctccgaggg ttcttcggcg cgtgcgaggc
     1921 ggccgacgtc gccgccggcg gcgtccacgt cgagctcacc ggcgagaacg tgaccgagtg
     1981 cctcgggggg agcgaagagg tgctcgacga gcacctcgag cagcgctacg agacggtctg
     2041 tgaccctcgg ctgaacgccc gccagtcact cgacctggcc ttcaacgtcg ccgggctgct
     2101 gcggggctga gcccccgagg caccaagacc gcgtcgttcg gtgcgacgat gacgacatga
     2161 gcttcgctga cggcggcgtc aaccaggggc tcctcgacga cctgtacgcc cggtaccgcg
     2221 acgaccccag ctcggtcccg gagcggtggc gccgcttctt tgaccccgcc cgtgggaacg
     2281 gggccgatcc ggccccgccg tccactccgc cggccgccga agccaccgtc gtgccggcac
     2341 cgccatcacc cccggcaccg ggcggcgcgc ccatcgacgc cccctcggcc gcggcggtcg
     2401 cgcccgtcga ggaggccgag gtgcttcgcg gcgccgcggc ccgcaccgtc gtgaacatgg
     2461 aggcgtccct cggagtcccg accgcgacgt cggtgcggac ggtgcccgcc aagctgctcg
     2521 aggtcaaccg ccagatcctc aacaaccatc tggggcgcac cgggcgcggg aaggtcagct
     2581 tcacccacct catcgcgttc gccgtcgtgc gggcccagcg cgacttcccc gggctgaacg
     2641 ccagctacga cgaggttgac ggccagccga cgatcacccg tcacgcccac gtgaacctcg
     2701 ggctggcggt cgacgtcacg aggaaggacg ggacccgcac cctcctcgtc cccaacatca
     2761 agacggccga ccagctggac ttcgcgcagt tctggtcccg ctacgaggac ctcatcgcca
     2821 aggtgcgcgc cgggaagctc tcccccgacg acttcaccgc cacgaccgcg acgatcacga
     2881 accccggcat gatcggaacc gtccactccg tcccgcgcct catggccggc caagggttca
     2941 tcgtcggcgt cggctcgatc ggataccccg ccga
//
LOCUS       DATCCH010000053         2078 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_504179, whole genome shotgun sequence.
ACCESSION   DATCCH010000053 DATCCH010000000
VERSION     DATCCH010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504179"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="VIJ44_01110"
     CDS             <1..374
                     /locus_tag="VIJ44_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018178137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="short-chain dehydrogenase"
                     /protein_id="HEY5164525.1"
                     /translation="ELGRYGVRSNAITPMARTRLTEQTPGLEDVVKAPTDAAKFDAWD
                     PANISPMIAYLGTADCPFTGETFFVQGDLVARYQPHVLAEVISNNGIRWTVERLIEEG
                     PKLAPGDDRRGGPGELARAAR"
     gene            complement(407..811)
                     /locus_tag="VIJ44_01115"
     CDS             complement(407..811)
                     /locus_tag="VIJ44_01115"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEY5164526.1"
                     /translation="MAKYQFLSDEWFAIVDQLVEEHGAEAPGSTDILMNVTVTDTPFG
                     AERHMHMGAKQGRGHWGIGHADGADLQLTTDYGTAKEVFVSGDPAAGMQAFMSGRVKI
                     QGDMAKLMAAQASGAAPGGATALQAAIQGITE"
     gene            895..1194
                     /gene="gatC"
                     /locus_tag="VIJ44_01120"
     CDS             895..1194
                     /gene="gatC"
                     /locus_tag="VIJ44_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003893732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEY5164527.1"
                     /translation="MSQRITRSDVEHVARLARLALTDDEIARFTTELGHILEHAADLA
                     TLDLDGVEPTAHPLPLANVTRQDEIRPPLPRDEVLAEAPAVEDGRFRVPRILDAP"
     gene            1191..>2078
                     /locus_tag="VIJ44_01125"
     CDS             1191..>2078
                     /locus_tag="VIJ44_01125"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY5164528.1"
                     /translation="MTAVDIAGSVRSGQRAARDVVEEHLTRVEQREAEIHAFTLVLAD
                     KARRAADDIDATVARGDDPGPLAGVPIAVKDNLCTRGTPTTCGSRILEGWRPPYTATC
                     VERILTAGAIVVGKANCDEFAMGSSTENSAFGPTRNPHDPTRVPGGSSGGSAAAVASG
                     MVPVGHAGDGGGSIRIPASECGLVGLKPSRARVPLGPEWGDVMGGLVCELAVTRSVRD
                     TAAVLDAVSGPAPGDPYAAPTPARRFLEELGIDPGRLRIGMQSAAFGAVVVTDPGCVA
                     ATEAAGALLEAIGHVVEPTH"
BASE COUNT          285 a          776 c          712 g          305 t
ORIGIN      
        1 tggagctcgg ccgttacggc gtccggtcga acgcgattac gcccatggcc cggactcgcc
       61 tcacggagca gacccccggc ctcgaggacg tggtgaaggc gccgaccgac gcggccaagt
      121 tcgacgcttg ggatccggcg aacatctcgc cgatgatcgc ctatctcggc acggccgact
      181 gtcccttcac cggtgagacg ttcttcgtgc agggcgacct cgtggcgcgg taccagccgc
      241 acgtcctcgc cgaggtgatc tcgaacaacg ggatccgatg gaccgtcgag cgcctgatcg
      301 aagagggccc caagctcgct cccggcgatg accgccgggg cggacccggg gagctggcgc
      361 gcgccgcccg ctgacgcgtc gatccacccc gcggtttcgc ccggggctac tcggtgattc
      421 cctggatggc cgcctggagc gcggtggcgc cccccggcgc cgcgccactg gcctgcgcgg
      481 ccatgagctt ggccatgtcg ccttggatct tcacccggcc tgacatgaac gcctgcatcc
      541 cggccgccgg gtcacccgac acgaagacct ccttggccgt cccgtagtcg gtcgtcagct
      601 gcagatccgc gccgtcggcg tggccgatcc cccagtggcc ccgtccctgc ttcgcgccca
      661 tgtgcatatg acgctcggcc ccgaacggcg tgtccgtgac cgtcacgttc atcaggatgt
      721 cggtgctgcc cggcgcctcg gcaccgtgct cctccacgag ctggtcgacg atcgcgaacc
      781 actcgtcgga gaggaactga tacttcgcca cgacgtcctc cgtgatctcg gtgggcagat
      841 cgtagccccg accctcggct ggtgatccgg tcgttcccgt agactcgcgg caccatgtcg
      901 caacggatca cacggtcgga tgtcgagcat gtcgcccgac tggcgcggct cgcgttgacc
      961 gacgacgaga tcgctcggtt cacgaccgag ctcggccaca tcctcgaaca cgccgcggat
     1021 ctcgcgacgc tcgacctcga cggcgtcgaa ccgacggcgc atcccctgcc gctcgccaac
     1081 gtcacgcgtc aggacgagat ccgcccgccg ttgccccgcg acgaagtgct cgccgaggcc
     1141 cctgcggtcg aggacggccg cttccgggtt ccccgcatcc tcgacgcccc gtgaccgccg
     1201 tcgacatcgc cggttccgtc cggtcaggcc aacgcgcggc ccgcgacgtc gtcgaggagc
     1261 acctcacccg ggtcgagcag cgcgaagccg agatccacgc gttcacgctg gtcctcgcgg
     1321 acaaggcccg gcgggccgcc gacgacatcg acgccaccgt cgcccggggc gacgaccccg
     1381 ggccactcgc cggcgtgccg atcgcggtga aggacaacct ctgcacccgg gggacgccga
     1441 cgacgtgcgg ttcgcggatc ctcgaagggt ggcgcccgcc gtataccgcg acctgcgtcg
     1501 agcggatcct caccgccggg gccatcgtcg tcggcaaggc gaactgcgac gagttcgcga
     1561 tgggttcctc gaccgagaac tccgccttcg gcccgactcg caacccgcac gatccgaccc
     1621 gagtcccggg cgggtcgagc ggcggctcgg ccgcggcggt ggcgtcgggc atggtgccgg
     1681 tcggacacgc cggtgacgga ggcgggtcga tccgcatccc ggccagcgaa tgcgggctgg
     1741 tggggctcaa gccgtcgcgg gcccgcgtcc cgctcgggcc cgaatggggc gacgtcatgg
     1801 ggggtctcgt gtgcgagctg gccgtgaccc gctcggtgcg cgacaccgcg gcggtactcg
     1861 acgcggtgtc gggaccggcc ccgggggatc cctacgcggc gccgaccccg gcccgtcggt
     1921 ttctcgagga gctcggcatc gaccccggtc gactgcgcat cgggatgcag agcgcggcct
     1981 tcggggcggt ggtggtcacc gatcccggct gcgtggccgc caccgaagcg gcgggtgcgc
     2041 tgctcgaggc gatcggccat gtcgtcgagc ccacccac
//
LOCUS       DATCCH010000054         3065 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_462971, whole genome shotgun sequence.
ACCESSION   DATCCH010000054 DATCCH010000000
VERSION     DATCCH010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462971"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VIJ44_01130"
     CDS             <1..215
                     /locus_tag="VIJ44_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiolase"
                     /protein_id="HEY5164529.1"
                     /translation="GDTLHFDSGKLPTNTHGGLLSHAYVLGIAHVVEVVRQLRGEAAA
                     QVPDATFGVYGGYTGPQAATLVLRRG"
     gene            226..654
                     /locus_tag="VIJ44_01135"
     CDS             226..654
                     /locus_tag="VIJ44_01135"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEY5164530.1"
                     /translation="MRPDFPLPDTTWEPTREFWAGAARHEVRIPRCDQCGRSCWYPRE
                     RCRFCEGTSFQWVTMSGRGTLFSWVVVTHAFLPQFADLVPFVPALVALDDDPAVRLAT
                     RVVDCDVDTLTFEMPLQVTFQPITFHGVDGQVTAPLFAPA"
     gene            672..941
                     /locus_tag="VIJ44_01140"
     CDS             672..941
                     /locus_tag="VIJ44_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4499 domain-containing protein"
                     /protein_id="HEY5164531.1"
                     /translation="MAVDRRWWLTNTGGIVVTGVLAAATGRPALQRLFHTAVAIHVGE
                     ALYSYSLARRAGFTTSAPRWALQTLGVGFPSLIALRAARQAASAQ"
     gene            complement(976..1794)
                     /locus_tag="VIJ44_01145"
     CDS             complement(976..1794)
                     /locus_tag="VIJ44_01145"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5164532.1"
                     /translation="MSTPAFQVDTHVHVVADDEGRYPLNPSGATGAWYREDPCSIGRL
                     VDLMDVAGVDAAVLVQGISAYRFDNRYTLDAAAQHPERCTSVVCVDLGGADPASEVTA
                     LLDRGAGGLRWVALHDGGVEEPRAVWDIVSDRRIPVVVTLLDRHLPGLAEALPRLPEV
                     LLALDHCGFADLSHGLPDALTALAAFPAVHLKVSTIALDAAAEHGDVRDFLSDLAACF
                     GATRLMWGSDYSQTHDRPYPALADYARFASSKLGDDDRALFLGRTACTVWPELQ"
     gene            complement(1814..2578)
                     /locus_tag="VIJ44_01150"
     CDS             complement(1814..2578)
                     /locus_tag="VIJ44_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007976912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5164533.1"
                     /translation="MGDRLQDKVALVTGAASGIGAACVARFAAEGAAVAGLDLQAQPA
                     DSAAAGWWTADVRDEATVERTVDAVTDQRGRIDVLVNAAGVLSIGTADEMDREEWDRV
                     LGINLTGTWLVSKHVVRRMVAQGSGSIVNLASIEGLIGFSGQTAYNVSKGGVVLLTRN
                     MATDFGPSGVRINCLCPGLIDTPLTEILHDPALAKVRERFVEWHTLGRAGRPEEVAAA
                     ALFLASDDASFVHGEALTVDGGLTAGRRFLNPGEEL"
     gene            complement(2600..>3065)
                     /locus_tag="VIJ44_01155"
     CDS             complement(2600..>3065)
                     /locus_tag="VIJ44_01155"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5164534.1"
                     /translation="SVVVGGWGNRTRRSVVALFGIFLLVAGQILLAVAPSYALGLSGM
                     FTMGAAYLLIATALNTSIQGRVDETHRGRAMSIYLMGLLAGVPLGALLQGKLASIIGL
                     RETVFGAAVLLGLLAAAVALTRDGFRPLDEEIHDEPGTDLLLNAPPSIASAD"
BASE COUNT          461 a         1099 c         1061 g          444 t
ORIGIN      
        1 ccggcgacac cctccacttc gactccggga agctcccgac caacacccac ggtgggctgc
       61 tctcgcacgc gtacgtgctc ggcatcgccc atgtcgtcga ggtcgtccgc cagctgcgcg
      121 gcgaagccgc agcccaggtg cccgacgcca cgttcggggt gtacggcggc tacaccggcc
      181 cccaggccgc cacactcgtg ctgcgtcgcg gctagcaacc tgacgatgcg acccgacttc
      241 ccgctccccg acaccacctg ggagccgacc cgcgagttct gggccggcgc tgcccgccac
      301 gaggtgcgca tcccccgctg cgaccagtgc gggcggtcct gttggtaccc gagggagcga
      361 tgccggttct gtgaaggcac gtcgtttcag tgggtgacga tgagtggccg gggcaccctc
      421 ttctcttggg tcgtcgtcac ccatgccttt ctcccccagt tcgccgacct cgtcccgttc
      481 gtcccggcgc tcgtcgcgct cgacgacgac cccgccgtcc ggctcgccac ccgcgtcgtc
      541 gactgcgacg tcgatacgct caccttcgag atgccgctcc aggtgacctt ccagccgatc
      601 acgttccacg gcgtcgacgg ccaggtcacc gcgccgttgt tcgcaccggc atgatcaccg
      661 acgacacttt cgtggcggtc gatcgtcgct ggtggctgac gaacaccggt gggatcgtcg
      721 tgaccggggt gctcgccgcg gccaccggcc gtccagccct ccaacggctg ttccacaccg
      781 cggtcgccat ccacgtcggt gaggccctct acagctacag cttggcgcgt cgggccgggt
      841 tcacgaccag cgcgccccgg tgggccctcc aaacgctcgg ggtcgggttc ccatcgctga
      901 tcgcactacg ggctgcgcgc caggctgcct ccgcccagta gccgagcggc ggccgagtgg
      961 gccgccgagt cccgttcact gcagctcggg ccacacggtg caggcggtcc ggccgaggaa
     1021 caacgcccgg tcgtcgtcac caagcttcga cgaggcgaac cgggcatagt ccgcgagggc
     1081 gggatacgga cggtcgtgag tctggctgta gtccgatccc cacatcagcc gggtggcgcc
     1141 gaagcaggcg gcgagatcgg acaggaagtc gcgcacgtcg ccgtgctcgg cagcggcatc
     1201 gagggcgatg gtcgaaacct tgagatggac cgcggggaac gccgcgagtg cggtcagcgc
     1261 atccggcagg ccatgcgaga ggtcggcgaa gccgcagtgg tcgagcgcga gcagaacctc
     1321 ggggagacgc gggagcgcct cggccaggcc cgggaggtgc cggtcgagca acgtgacgac
     1381 gacggggatc cggcggtcgg agacgatgtc ccagaccgct cgtggctcct cgacgccgcc
     1441 gtcgtgcagc gcgacccagc gcaacccgcc tgcgccccga tcgagcagtg cggtgacctc
     1501 ggaggcgggg tcggctcctc ccaggtcgac acacaccacg ctggtgcacc gctccgggtg
     1561 ctgggcggcc gcatcgaggg tgtagcggtt gtcgaatcgg tacgcgctga tgccctggac
     1621 caggaccgcg gcgtcgaccc cggcgacgtc catgaggtcg acgaggcgcc cgatcgagca
     1681 ggggtcctct cggtaccagg cgccggtggc accgctcggg ttgagcgggt agcgcccctc
     1741 atcgtcggcg accacatgga cgtgcgtgtc gacctggaac gcaggggtgc tcaccgcgga
     1801 tcgggcgcca cgttcagagc tcctcgccgg ggttgaggaa gcgtcgaccg gcggtgagtc
     1861 ccccgtcgac cgtcagggcc tcgccgtgca cgaacgaggc gtcatcggag gcgaggaaca
     1921 gggcggcggc cgcgacctcc tcggggcgac cggcccggcc gagggtgtgc cactcgacga
     1981 accgctcccg gaccttcgcc agggccgggt cgtggaggat ctcggtgagt ggcgtgtcga
     2041 tgaggcccgg gcagaggcag ttgatgcgca caccggacgg cccgaagtcg gtcgccatgt
     2101 tgcgcgtgag cagcaccacg ccgcccttgg agacgttgta ggccgtctgg cccgagaacc
     2161 cgatcagccc ttcgatgctg gccaggttca cgatgctccc tgagccttgc gcgaccattc
     2221 gacgcaccac gtgcttcgag accagccagg tgccggtcag gttgatccca agcacccggt
     2281 cccactcttc acggtccatc tcgtcggcgg tgccgatgct caacaccccg gccgcgttca
     2341 cgagcacgtc gatgcgaccg cgctggtcgg tcaccgcatc gacggtccgc tcgaccgtcg
     2401 cttcgtcccg tacatcggcg gtccaccagc ccgcggccgc cgagtcggcc ggctgggcct
     2461 gcagatcgag acccgcgacg gccgcaccct ccgccgcgaa gcgggcgacg cacgccgcgc
     2521 cgatgcccga cgcggcgccg gtcacgagcg cgaccttgtc ctgcaaccgg tcacccatgt
     2581 tcgttccctc aggtcgtgtt cagtcggcgc tggcgatcga cggtggagcg ttgaggagca
     2641 ggtcggttcc cggctcgtcg tggatctcct cgtcgagggg acggaacccg tcgcgcgtga
     2701 gcgcgacggc ggcggcgagc aatccgagga ggaccgccgc gccgaatacc gtctcgcgca
     2761 gcccgatgat cgaggccagc ttgccctgga gcagggcgcc gagcgggaca ccggcgagca
     2821 ggcccatgag atagatcgac atcgcccgcc cgcggtgcgt ctcgtcgacc cgcccctgga
     2881 tggaggtgtt cagcgcggtt gcgatcagca ggtacgccgc gcccatcgtg aacatgccgg
     2941 agaggcccag cgcgtagctc ggagccaccg ccagcaggat ctgcccagcc acgaggagga
     3001 agatgccgaa cagggcgacg accgaacggc gggtccggtt gccccagcct ccaaccacga
     3061 ccgag
//
LOCUS       DATCCH010000055         2526 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694 k141_3104,
            whole genome shotgun sequence.
ACCESSION   DATCCH010000055 DATCCH010000000
VERSION     DATCCH010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3104"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="VIJ44_01160"
     CDS             complement(<1..531)
                     /locus_tag="VIJ44_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HEY5164535.1"
                     /translation="MTPAPLGADFLDRRLLFFTGKGGVGKSTVAAATALLAAERGRRV
                     LMIEVDSKGNLASLFEHQPVGFKPTEVYPGVFAMQMRTEAALREYLKLNLRIPVLGRL
                     GPLANVLEFVATAAPGVKEILTVGKVCWEAREALEGRADWDLIVVDAAATGHVVAQLD
                     SPRAFQELVQVGPVRQQ"
     gene            complement(536..766)
                     /locus_tag="VIJ44_01165"
     CDS             complement(536..766)
                     /locus_tag="VIJ44_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018561495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3107 domain-containing protein"
                     /protein_id="HEY5164536.1"
                     /translation="MDVRIGVIWSPKELTLEVDGDAAQLESSINDALNGDGKVLWLTD
                     VHGRRVAVPADRVAYVEIDADGNAKRVGFGPG"
     gene            complement(814..1959)
                     /locus_tag="VIJ44_01170"
     CDS             complement(814..1959)
                     /locus_tag="VIJ44_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HEY5164537.1"
                     /translation="MPAHDAEVRAALRGVEDPELAASVVDLGMVAAVARDGGTVVVQL
                     ALALPEDTWPPDELIRRVRATVEAIPGITRVDVELRPMTPQEESDAARVLRGDPPRNP
                     LAVVDADAEAPRPPRRNPFTDSSTRILAISSGKGGVGKSSVSTNLAIGLAARGHRVAA
                     VDADVWGFSLPRMLGIDRPPGLVDDVIVPPSAHGVRLVSMGFFARDDQAVIWRGPMLH
                     KALEQFLTDVHWGNPDVLVVDLPPGTGDVALSMAQLLPHAEVVVVTTPQPAAQRVAQR
                     SAAMAEKVDLPVVGVIENMSWFTGDDGKRYEIFGSGGGRALADTLGVPLLGQVPLVSA
                     LREGGDTGRPISVTDPDSEAAQVFTAIAERLDAATPRRIRHPELRIG"
     gene            2016..>2526
                     /locus_tag="VIJ44_01175"
     CDS             2016..>2526
                     /locus_tag="VIJ44_01175"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY5164538.1"
                     /translation="MAAEPVPKPVSRRVPPPEGSERGLTADEFTAAVAAVTSAFGDPT
                     RREIYLFARDASDGVTTAEVAEQFALHPNVARHHLEKLTGGGYLMVAISRPDDGNRPA
                     GRPSKRYLATGRDTLTLPLRQDDVLAGLLAGALDALGADAAETLADQVGYEYGRTLAA
                     RMDPGEGHRS"
BASE COUNT          367 a          923 c          893 g          343 t
ORIGIN      
        1 ctgctggcgg accgggccga cctggaccag ctcctggaac gcacgcggcg agtcgagctg
       61 ggcgaccacg tggccggtgg cggcggcgtc gacgacgatg aggtcccagt ccgcgcgacc
      121 ttcgagagct tcccgggcct cccagcacac cttcccgacg gtgaggatct ccttcacccc
      181 tggggccgcg gtggccacga actccaacac gttcgccaac ggtccgagac ggccgagcac
      241 cgggatccgc aggttgagct tcaggtactc gcgtaacgcg gcctcggttc gcatctgcat
      301 cgcgaagacg ccggggtaga cctcggtcgg cttgaacccg acgggttgat gctcgaacag
      361 cgacgcgagg ttgcctttgg agtcgacctc gatcatcagt acccgtcggc cccgttcagc
      421 cgcgaggagc gccgtcgcgg ccgcgacggt gctcttgccg accccaccct tgccggtgaa
      481 gaagagcagg cgacggtcga ggaagtccgc gccgagaggc gccggggtca ccgattcagc
      541 cgggaccgaa cccgacccgc ttggcgttcc cgtcggcgtc gatctcgacg tacgcgaccc
      601 ggtcggccgg caccgcgacc cgtcggccgt ggacgtcggt cagccagaga accttcccgt
      661 cgccgttcag ggcgtcgttg atcgacgatt cgagctgcgc ggcgtcgccg tcgacctcga
      721 gcgtcagctc cttcggggac cagatgacac cgatgcgcac gtccatggcc gggacactac
      781 cggtcggtca ccgtgcgtcc cgacgcgtgt cagtcatccg atccggagct ccggatgacg
      841 gattcgcctc ggggtggcgg cgtcgagacg ctcggcgatg gcggtgaaca cctgcgccgc
      901 ttcgctgtcg gggtcggtca cgctgatggg ccggccggtg tcgccgccct cgcggagcgc
      961 ggagaccagg ggaacctggc cgaggagcgg cacgccgagc gtgtcggcca gcgctcgccc
     1021 gccgcccgag ccgaagatct cgtagcgctt cccatcgtcg ccggtgaacc aggacatgtt
     1081 ctcgatgacg ccgaccaccg gcaggtcgac cttctcggcc atcgccgcgc tgcgttgcgc
     1141 gacacgctgc gccgccggtt ggggcgtggt gacgaccacg acctcggcgt ggggcagcag
     1201 ctgggccatc gagagcgcca cgtcgccagt gccggggggc aagtcgacga cgagcacgtc
     1261 ggggttcccc cagtggacgt cggtgaggaa ctgctccagc gccttgtgca gcatcggtcc
     1321 gcgccagatc acggcctgat cgtcgcgggc gaagaacccc atcgagacga gccggacacc
     1381 gtgggcgctg ggcgggacga tcacgtcgtc gacgagtccc gggggccggt cgatgccgag
     1441 catgcggggc agcgagaacc cccagacgtc ggcgtcgacc gccgcgacgc ggtggccacg
     1501 cgccgcgagc ccgatcgcga ggttcgtact caccgacgac ttccccacac cgccctttcc
     1561 ggacgagatc gccaggatgc gcgtcgacga gtcggtgaac gggttccggc gggggggccg
     1621 gggcgcttcg gcgtcggcgt cgacgacggc gagcgggttc cggggcgggt ccccgcgcag
     1681 gacccgcgcc gcgtcggact cctcttgggg ggtcatgggg cgaagctcga cgtcgacccg
     1741 ggtgatgccg gggatcgcct cgaccgtggc ccgaactcgc cggatcagct cgtcgggcgg
     1801 ccaggtgtcc tcgggcagcg ccaaggccag ctggaccacg acggtcccgc cgtcgcgcgc
     1861 caccgcggcg accatcccga ggtccacgac cgaggccgcc agctcgggat cctcgacccc
     1921 gcgcagggcg gcccgaacct cagcgtcgtg ggcgggcacg ggcagcctcc gggagggacg
     1981 ggatttccac tacgggcata ggtagaatac cggtcgtggc cgccgagccc gtcccgaagc
     2041 ctgtcagtcg ccgcgtcccc ccgccggaag ggtcggagcg gggcctgacg gccgacgagt
     2101 tcactgccgc cgtcgccgcg gtgaccagcg cgttcggcga ccccacccgt cgggagatct
     2161 acctcttcgc ccgcgacgcc tcggacgggg tcacgaccgc cgaggtcgcc gagcagttcg
     2221 cgctgcaccc gaacgtggcc cgccaccatc tcgagaagct caccggtggc ggctacctga
     2281 tggtcgccat cagccgtccc gatgacggca accgtcccgc cggtcgccct tcgaagcggt
     2341 acctggccac cggccgagac accctcaccc tcccgctccg ccaggacgac gtgctcgccg
     2401 gcctcctcgc gggcgcgctc gacgcgctcg gcgccgacgc cgccgaaacc ctcgccgatc
     2461 aggtcggcta cgagtacggc cgcaccctcg cggcccggat ggaccccggc gaagggcacc
     2521 gctcgg
//
LOCUS       DATCCH010000056         2603 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_210585, whole genome shotgun sequence.
ACCESSION   DATCCH010000056 DATCCH010000000
VERSION     DATCCH010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210585"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..547)
                     /gene="def"
                     /locus_tag="VIJ44_01180"
     CDS             complement(47..547)
                     /gene="def"
                     /locus_tag="VIJ44_01180"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015103659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEY5164539.1"
                     /translation="MFGDPVLRQPAREVTELNGELARLVDTMIETMNEAHGVGLAAPQ
                     VGVQKRLYTYDVGEGPAVLINPEIVDTRDDWTFEEGCLSVPGLQFDIVRPKVVTVRGV
                     DLDGNEIVIEGDELMGRLIQHEVDHLDGVLLLQRLDPERRREALRELRTRAFDGFTPV
                     GSAHHL"
     gene            complement(628..1020)
                     /locus_tag="VIJ44_01185"
     CDS             complement(628..1020)
                     /locus_tag="VIJ44_01185"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5164540.1"
                     /translation="MDDERLIFLALWVSDLEASAGFYRETLGVPLHVGFNEPTGDPWT
                     DGRHYEHSWRDGAYLHFALFPIRDGVSPTRGAELSFATTDLDAKHEELVSNGVAVVHG
                     PRTWESAGLRMARYRDPDGNVVGFTERI"
     gene            complement(1099..>2603)
                     /locus_tag="VIJ44_01190"
     CDS             complement(1099..>2603)
                     /locus_tag="VIJ44_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164541.1"
                     /translation="LRAASPPNIVDPRQAPSEPDLAVHPSLDPPLPLPDGTEPRLVIW
                     PPARDRAALVQSLLAPEGSTIVVVPDRAEQQHLVATARAAGRSVVEVRGDDPAGTRTA
                     AWRDARRGACVVLGGRIAVFAPVPDLQAVVVLDDADEALVEERAPTWHARAVAQERAR
                     RADASMTIITPAPTVEASVTSPVTTALPPATSRAGWPLVEVVDRRSEPPGLGLLGERL
                     ATALRTVRAEKRRTICVLNRRGRARLLICRQCGAPARCEHCGAAVVETDGGLACPRGD
                     GSRPFVCADCGASDLRPARPGVRRLRDDLAALLPRARVSVAERGVHPDDDADVVVGTE
                     AALHGVLNQDHPVGLVAFLDFDQELLAPRYRADEQALWLLVRAARLCGDRRSGSRILV
                     QTRLPDHEVLAAARTGDPMPVVDADAVRRETLAFPPFGGLAALDGDTDAVAAAATILR
                     DHLEVLGPVDGRALLRAASPTALADALAATDLTAARAHGRLRIEVDPQRV"
BASE COUNT          361 a          841 c         1031 g          370 t
ORIGIN      
        1 cgcggcggcc ggggtaccga agaagacgag tcgcacccgc ggcggcttac aggtggtggg
       61 cgctcccgac gggggtgaag ccgtcgaagg cgcgggtgcg cagctcgcgc agcgcctccc
      121 ggcggcgctc agggtcgagc cgctgcagga gcaggacacc gtcgaggtgg tcgacttcgt
      181 gctggatgag tcgacccatg agctcgtcgc cttcgatgac gatctcgttc ccgtcgaggt
      241 cgacgccccg cacggtcacg accttcggtc gcacgatgtc gaattgcaat cccgggaccg
      301 agaggcagcc ttcctcgaag gtccagtcgt cgcgggtgtc gacgatctcg ggattgatga
      361 ggaccgcggg cccttcgccg acgtcatagg tgtagaggcg cttctggacg ccgacctggg
      421 gtgcggccag gccgacaccg tgggcttcgt tcatggtctc gatcatggtg tcgaccaggc
      481 gcgccagctc tccgttcagc tcggtgacct cgcgtgccgg ttggcggagc acggggtcac
      541 cgaacatgcg gattacgaac gtgttcaccg tgccaggcta ccagcggcct ttgtgggatt
      601 ggtgggtcgg tcgtggtccg ggcgcggtca gatccgttcc gtgaacccga ccacgttccc
      661 gtccgggtcg cgatacctgg ccatccgtaa cccggcgctc tcccaggtcc ggggcccgtg
      721 cacgaccgcg acgccattgg agacgagttc ctcgtgtttg gcgtcgaggt cggtggtcgc
      781 gaacgaaagc tccgcgccgc gcgtggggct gacgccgtcg cggattggaa acaaggcgaa
      841 gtggaggtag gcgccgtcgc gccacgaatg ctcgtagtgg cgcccgtcgg tccacgggtc
      901 tccggttggc tcgttgaagc ccacatggag ggggacgccg agagtttccc ggtagaagcc
      961 tgccgacgct tccaggtcac tcacccagag ggcgaggaag atgaggcgct catcgtccat
     1021 gtcgatcctc agctccggcg tctgctggac gagcctatgc aggaagctgg ctcgcgaccg
     1081 gcgggcgcgg tcgtcgcgtt agacccgttg cgggtcaacc tcgattcgga gccgtccgtg
     1141 cgcccgagcc gcggtgaggt cggtggcggc aagcgcgtcg gcgagcgcgg tcggcgacgc
     1201 ggcgcgcagg agtgcccgac cgtcgacggg gccgagcacc tcgagatggt cgcgcaggat
     1261 ggtggccgcg gcggcgactg cgtcggtgtc gccgtcgagc gcggcgaggc ctccgaacgg
     1321 tgggaacgcc agggtctcgc gccgcacggc gtcagcgtcg acgaccggca tcgggtcgcc
     1381 ggtgcgggcc gcggcgagga cctcatggtc cgggagacgg gtctggacca ggatgcggga
     1441 tccggaccgt cgatccccgc aaaggcgagc ggcgcggacg aggagccaga gtgcctgctc
     1501 atcggctcgg tatcgcggtg cgagtagctc ctggtcgaag tcgaggaacg cgacgaggcc
     1561 caccgggtgg tcctggttca agacgccgtg cagcgcggct tcggtgccga ccacgacgtc
     1621 ggcgtcgtcg tcggggtgca cgcctcgctc ggcgacgctg acccgggcgc gagggaggag
     1681 cgcggcgagg tcgtcgcgga gacggcggac gccggggcgg gccgggcgca ggtcgctggc
     1741 cccacagtcg gcgcacacga acgggcggct cccgtcgccg cggggacagg cgagcccccc
     1801 gtcggtctcg accaccgccg cgccgcagtg ctcgcaacgc gccggtgcac cgcattggcg
     1861 acagatgagc aggcgcgccc gcccgcgccg gttgaggacg cagatggtgc gtcgtttctc
     1921 ggcgcgcacg gtgcgcagcg cggtggcaag ccgctccccg agcaggccga gcccgggcgg
     1981 ctcggagcga cgatcgacca cctcgacgag gggccagccg gcccggctgg tggcgggcgg
     2041 cagcgcggtg gtgacggggc tcgtgacaga ggcctccacg gtgggcgccg gggtgatgat
     2101 tgtcatcgac gcgtcggccc ggcgggcccg ttcctgggcc acagcccggg catgccaggt
     2161 cggggcgcgt tcctcgacga gggcttcgtc ggcgtcgtcg aggacgacga ccgcctggag
     2221 gtcgggtacc ggggcgaaca cggcgatgcg gccaccgagg acgacacacg cgccgcgtcg
     2281 cgcgtcgcgc catgcggcgg tgcgggtccc ggccgggtcg tcgccccgca cctcgacgac
     2341 ggaccgtccg gcggcccggg cggtggccac caggtgctgc tgctcggccc ggtcggggac
     2401 aacgacgatg gtggagcctt ccggcgcgag gagtgattgc acgagggcgg ctcggtcccg
     2461 ggcggggggc cagatgacga gacggggctc ggttccgtcc gggagcggaa gtggcggatc
     2521 gagtgagggg tggaccgcga gatcgggttc cgagggcgcc tggcgcgggt cgacgatgtt
     2581 cggcggagac gcggcgcgca gga
//
LOCUS       DATCCH010000057         2314 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_90117, whole genome shotgun sequence.
ACCESSION   DATCCH010000057 DATCCH010000000
VERSION     DATCCH010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90117"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..951)
                     /locus_tag="VIJ44_01195"
     CDS             complement(25..951)
                     /locus_tag="VIJ44_01195"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014143121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEY5164542.1"
                     /translation="MALPEEVKALASRVRNWGRWGDDDEIGTLNLITDDVVKAAAAEI
                     QTGRRLALGIPMNAEGPQTGAIPGRDNPTHEMIMVDTPLTGDASNFTTSDDKVAMALQ
                     AATHWDALAHVSYEGRLYNGVSSSVITDAGAARMGIDQIRTLVGRGVLLDLPAAKGVD
                     RLEGGYPVTGADLDAAAELGKVTVRSGDIILLRTGQIRWFLEGDRDRYGAPAPGPSLQ
                     TVDWFHDHDIAAVATDNLTFEVYPSEREDALLPVHLLHLVDMGMTQGQNWNLEELAVD
                     CAGDQRYSFFLDASPMPVTSAVGSPVNPIVVK"
     gene            complement(1027..2142)
                     /locus_tag="VIJ44_01200"
     CDS             complement(1027..2142)
                     /locus_tag="VIJ44_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014143117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEY5164543.1"
                     /translation="MRGLVYDGSKAAIRDDVEVRAPGPTEVKVRIVAAGLCHSDLSVI
                     DGTIPWPTPAVLGHEGAGVVDEVGEAVTHVAPGDHVVLHTLAYCGACKWCNTGRPAWC
                     RKSISNASQPFTVGGEAAWNFAAASFFADYTVVRGIQCVKIDPSIDLSVACLIGCGVL
                     TGIGSVWNRTNLGQGDTCAVFGVGGVGLNVIQAAKVRGARRIFAIDTVADKEAAARSF
                     GATDFILAGPGVDVVARIRQELPTEIENVVGSFGEGGVDYAFDCVGHPEILNSALEML
                     DWGGTAVAVGVPAPTATVPARITNFTHVERALIGSRAGSHRPHWDIPLIVDAYRRGQI
                     KLDELVSARYPIEDWETAVRDLHDGKLARGVLTVSPA"
     gene            complement(2168..>2314)
                     /locus_tag="VIJ44_01205"
     CDS             complement(2168..>2314)
                     /locus_tag="VIJ44_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018092256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY5164544.1"
                     /translation="EAGHVGVNTAQRNHEAPFGGFKMSGVGRDGGAFGLHAYTELQSV
                     IWPG"
BASE COUNT          349 a          773 c          804 g          388 t
ORIGIN      
        1 cggtcgtcga agcggcccat ctctctactt cacgacgatc gggttgaccg ggctgccgac
       61 ggcgctcgtg accggcatcg gggaggcgtc gaggaagaag gagtagcgct ggtcgccggc
      121 gcagtcgacc gcgagctcct cgagattcca gttctggccc tgggtcatcc ccatgtcgac
      181 gaggtgcagg aggtgcaccg gcaagagggc gtcttcgcgt tcggaggggt acacctcgaa
      241 ggtcaggttg tcggtcgcga cggcggcgat gtcatggtcg tggaaccagt cgaccgtctg
      301 cagcgacggg ccgggtgcgg gcgccccgta ccggtcccgg tcgccttcca ggaaccatcg
      361 gatctggccg gtccgcaaca agatgatgtc gcccgaccgg acggtgacct tccccagctc
      421 ggccgcggcg tcgagatccg cgccggtcac gggatagccg ccttcgagcc ggtcgacacc
      481 cttggccgcg ggaaggtcga gcaggactcc ccggccgacc agggtgcgaa tctggtcgat
      541 tcccatccgc gccgcccccg cgtcggtgat gacactggac gacactccgt tgtagagccg
      601 accctcgtag ctgacgtgcg cgagggcatc ccagtgcgtg gcggcctgga gggccatcgc
      661 gaccttgtcg tcgctggtcg tgaagttcga cgcgtcgccg gtgagcggtg tgtcgaccat
      721 gatcatctcg tgggtcgggt tgtcacggcc ggggatcgcg ccggtctgtg gcccctcggc
      781 gttcatgggt atcccgagcg ccagtcgtcg tccggtctgg atctcggctg ccgccgcctt
      841 gaccacatcg tcggtgatga ggttgagggt gccgatctcg tcgtcatcgc cccaccgccc
      901 ccagttccga acccgcgacg cgagtgcttt cacctcctcg ggcagtgcca tgtcacgctc
      961 cctgctggac ggttcgggcg tcgacggttc ccacgcacgt catgaccggg ggtgtcggct
     1021 gttgtctcaa gcgggcgaga cggtgaggac gccccgggcg agcttgccgt catggaggtc
     1081 gcgcaccgcg gtctcccagt cctcgatcgg gtagcgagca ctcacgagct cgtcgagctt
     1141 gatctgaccg cggcggtagg cgtcgacgat gagggggatg tcccaatggg gccggtgaga
     1201 gccggcccgg gagccgatga gggcccgttc gacgtgggtg aagttcgtga tgcgcgccgg
     1261 gaccgtcgcg gtcggcgcgg gcacacccac tgcgacggcg gtgccgcccc agtcgagcat
     1321 ctcgagcgcg gagttgagga tctccgggtg accgacgcag tcgaacgcgt agtcgacgcc
     1381 gccttcaccg aatgacccca ccacgttctc gatctcggtg ggaagctcct ggcggatgcg
     1441 ggccacgacg tcgacgccgg gaccagcgag gatgaagtcg gtcgcaccga agctgcgggc
     1501 ggcggcctcc ttatcggcca ccgtgtcgat ggcgaagatc cgacgggcgc cgcggacctt
     1561 ggccgcctgg atgacattga gcccaacgcc gccgacgcca aacacggcgc aggtgtcgcc
     1621 ctgtccgagg ttggtccggt tccagaccga gccgatcccg gtcaggactc cacagccgat
     1681 gaggcaggca acggacaggt cgatgctggg atcgatcttc acacactgga tgccacgaac
     1741 gacggtgtag tcagcgaaga acgaggcggc cgcgaagttc catgctgcct cgccaccgac
     1801 ggtgaacggt tggctggcgt tgctgatgct ctttcgacac caggccggcc ggccggtgtt
     1861 gcaccacttg cacgctccgc agtacgcgag cgtgtgcaac accacgtggt cgcccggagc
     1921 gacgtgcgtg acggcctcgc cgacctcgtc gacgacaccg gcgccctcat ggccgagcac
     1981 ggccggcgtg ggccacggga tggtgccgtc gatgacgctc aggtcgctgt gacacaaccc
     2041 ggcggcgacg atgcggacct tcacctcggt ggggccgggg gcacgcacct cgacgtcgtc
     2101 gcgaatcgcg gccttgctcc cgtcgtagac caggcccctc atgcgatgtg tcccttccgg
     2161 atcgggctca tcccggccag atcacgctct gcagctcggt gtaggcgtgg agcccgaacg
     2221 ctccgccgtc acgaccgaca ccgctcatct tgaacccgcc gaacggagct tcgtggttgc
     2281 gttgcgccgt gttgaccccg acatggcccg cctc
//
LOCUS       DATCCH010000058         2116 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_297053, whole genome shotgun sequence.
ACCESSION   DATCCH010000058 DATCCH010000000
VERSION     DATCCH010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297053"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="VIJ44_01210"
     CDS             complement(<1..410)
                     /locus_tag="VIJ44_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020549298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEY5164545.1"
                     /translation="MPETLGYRIKNRLLGPPIDTEQLVHERLGKPTALAVFASDNLSS
                     VAYGTEQILTQLVPAIGVAAFALVMPITIALLVVLAFLILSYRQTIKAYPSAGGAYIV
                     TRDNFGLLPAQVAGVALLTDYVLTVAVSVAAGTAA"
     gene            597..1265
                     /locus_tag="VIJ44_01215"
     CDS             597..1265
                     /locus_tag="VIJ44_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011981813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEY5164546.1"
                     /translation="MHVIVVGCGRVGSELAVSLDQGGHSVAIMDKNQVAFRKLPDGWG
                     GRTVHGFGFDRDHLEAAGVHEAGALAAVTSGDNSNILAARIARETFEIPRVVARIYDP
                     RRALIYQRLGIATVATVTWTTDQVMRRLFEDNSVTEWSEPSGQVVLVERALPDGWAGQ
                     PLAPLSSGDRFRLVSMTRSGQAQLAGNDLIGQEGDLLHIAVRKDALDELSELLSRPPT
                     SSTH"
     gene            1267..1929
                     /locus_tag="VIJ44_01220"
     CDS             1267..1929
                     /locus_tag="VIJ44_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018352889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEY5164547.1"
                     /translation="MKVTIAGAGNVGCYIASDLREAGHDVLMLDKSPTLVERLRPTLD
                     VNWFVGDACEVSSLHSAGIDGSDVMVAATGDDEDNLVVSLLAKQEFAIPRVVARVNHP
                     KNQWLFNETWGVDVSVSTPHLLSALVEEAVSVGSLVRLLQFEGGQARLVEVTLADGSP
                     AAGRSIADLAVPRDATVVAVVREGHVVVPRGDTVVNAGDEVLALVTPDSEEPVRSILI
                     GT"
BASE COUNT          334 a          741 c          726 g          315 t
ORIGIN      
        1 gcggcggtgc ccgcagcgac ggaaaccgcg accgtgagca cgtagtcggt gagcaacgcg
       61 accccggcga cctgcgccgg gaggagcccg aagttgtcgc gagtcacgat gtaggccccg
      121 cccgcgctcg ggtacgcctt gatcgtctgt cggtacgaca ggatcaggaa cgccaacacc
      181 acgagcagcg cgatcgtgat cggcatcacg agcgcgaacg ccgcgacacc gatcgcgggg
      241 accagctggg tgaggatctg ctcggtgccg tacgcgaccg atgacaggtt gtccgacgcg
      301 aacacggcca gagcggtggg cttgcccagc cgttcgtgca ccagctgctc ggtgtcgatc
      361 gggggtccaa gcagccggtt cttgatccgg tacccaagcg tctcgggcac ctcgaccgag
      421 gcctcggggg gtggcggaga cggcgcggtc gccgtctcgg ccgctcggag ttcggactcg
      481 gtcttcatcg ggctcccaga cggcgcgacg gcgaagcgcg ccgtcagtgt ggcatgacgt
      541 cgagacccac ggtcacccga gaagaggcgg gactcggggt gtgccaggat cggcccgtgc
      601 acgtcatcgt cgtgggctgc ggtcgggtcg gctccgagct cgccgtctcg ctcgatcagg
      661 gcggccacag cgtcgcgatc atggacaaga accaggtcgc gttccgaaag ctgcccgacg
      721 gctggggtgg ccgcaccgtc cacgggttcg gcttcgaccg agaccacctc gaggccgccg
      781 gggtccacga agccggggcg ttagctgcgg tcaccagcgg cgacaactcc aacatcctcg
      841 ccgcccggat cgcccgggag accttcgaga tcccgcgggt cgtcgcccgc atctacgacc
      901 ctcgccgggc gctcatctac cagcgcctcg ggatcgccac cgtcgcgacc gtcacctgga
      961 ccaccgatca ggtgatgcga cgtctgttcg aggacaactc ggtgacggaa tggtccgagc
     1021 ccagcgggca agtcgtcctg gtggagcggg ccctcccgga cggatgggcc ggccaacccc
     1081 tcgcgcccct ctcgagcggt gaccgcttcc ggctcgtctc gatgacccgg tccggtcagg
     1141 ctcagctcgc cggcaacgac ctcatcggcc aggaaggtga cctcttgcac atcgccgtcc
     1201 gcaaggacgc cctcgacgag ctgtcggagc tgctgagccg tccgccgacc agctcgacgc
     1261 actgacatga aggtcaccat cgccggagcc ggcaacgtgg gctgctacat cgccagtgac
     1321 ctgcgcgaag ccggtcacga cgtgctgatg ctcgacaaga gcccgactct cgtcgagcgt
     1381 ctccgtccga cgctcgacgt caactggttc gtcggcgacg cctgcgaggt cagcagcctc
     1441 cactcggccg ggatcgacgg cagcgacgtg atggtggccg cgaccgggga cgacgaagac
     1501 aacctcgtcg tctcgctcct ggcgaagcag gagttcgcca tcccgcgggt cgtcgcccgg
     1561 gtgaaccatc cgaagaacca gtggctgttc aacgagacct ggggtgtcga cgtgtcggtc
     1621 tcgacccccc acctgttgag cgccctcgtc gaggaggccg tgtcggtcgg ttcgctcgtc
     1681 cgcctcctgc agttcgaagg cggacaggcc cgcctggtcg aggtcacgct ggccgacggt
     1741 tcgccggccg cggggcgcag catcgccgac ctcgccgtgc ctcgcgacgc gaccgtggtg
     1801 gccgtcgtcc gggaaggcca tgtcgtcgtg ccacggggcg acacggtcgt caacgccggc
     1861 gacgaggtcc tggcgctcgt cacccccgac tccgaagaac cggtgcggtc catcctcatc
     1921 ggcacctgac gtcccgttgg ccatcccact ggcgggtcgc gccgaccgcc ccgacagagt
     1981 gtcagggcgg aaacggtacg cttcgacgag cgtctgatcg acgacgacgg acctcgagat
     2041 cggaccgcag ccggtggagc cagaaccgca ccctgccatg agcgagcctc ggctcgggtt
     2101 cggtgggctg gcgctc
//
LOCUS       DATCCH010000059         2057 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_297228, whole genome shotgun sequence.
ACCESSION   DATCCH010000059 DATCCH010000000
VERSION     DATCCH010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297228"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VIJ44_01225"
     CDS             complement(<1..256)
                     /locus_tag="VIJ44_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020392908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 domain-containing protein"
                     /protein_id="HEY5164548.1"
                     /translation="MSHPAPQTDGPQIDPRGPRFAAILTTILLAVVLLTQSPWLLAVQ
                     AVLFAVGAFAGVQRTPYAALFRRFVRPRLAPPAELEDARPP"
     gene            complement(495..944)
                     /locus_tag="VIJ44_01230"
     CDS             complement(495..944)
                     /locus_tag="VIJ44_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003988378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEY5164549.1"
                     /translation="MNPGLVLLVVVLVAATGFGVWRRLTDGRIRGVDARSTSSTADEN
                     HAVPSLTAAELGGALGRRATLVQFSTAFCQPCRATRRILGEVAEQVEGVAHLEVDAEA
                     HLDLVRRLDVMRTPTVFVLDGSGRVVRRAVGLPRKADVVAALGEAIP"
     gene            complement(1075..1335)
                     /locus_tag="VIJ44_01235"
     CDS             complement(1075..1335)
                     /locus_tag="VIJ44_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEY5164550.1"
                     /translation="MTVTLRYWAAAKEAAGLAEDAFAGPATVAELLAAAVAAHPDDGR
                     LARVLGRCAFLVDGDPVGTRPPDSVALSDGAVVEALPPFAGG"
     gene            complement(1332..>2057)
                     /locus_tag="VIJ44_01240"
     CDS             complement(1332..>2057)
                     /locus_tag="VIJ44_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016644790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY5164551.1"
                     /translation="SSLTRVTRVLSDRAGVVAFDFRGHGRSQGYSTLGDLEVLDLDAA
                     VRWARLLGYRSVATVGFSMGASVVIRHAALYRGVDAVVAVSGPARWYYRGTATMRRLH
                     FLVEHPIGRLFARVAMGTRIHDEGWDPVPAEPRAVAGDVAPTPLLVVHGDQDRFFPLE
                     HAEELAAAGGRTTELWVESGFGHAEAAIDDELTRRIAAWVRAVVASATPLHPAGGGSP
                     GVPEAFGGAAAPVRRVKRGQVGA"
BASE COUNT          310 a          741 c          792 g          214 t
ORIGIN      
        1 gcggcgggcg ggcgtcctcg agctcagccg gcggcgcgag gcgcgggcgg acgaaccgcc
       61 ggaacaacgc cgcgtagggc gtgcgctgga ccccggcgaa ggcgccgacg gcgaagagga
      121 cggcctgcac ggcgagcagc cacgggctct gggtgagcag gacgacggcg agcaggatcg
      181 tcgtgaggat cgcggcgaag cgcggccctc gcgggtcgat ctggggaccg tcggtctggg
      241 gagcagggtg cgacatgggt actcctgatg ggagccggca cgggagggtg tgcgggtgcg
      301 ggcagcaggc ggtcgcgagg accagcgggg tacccggtca ggaggacgga caggcgcagg
      361 aggtgctgcg gcacaggtcg actgcgcgcc gcttcgtgag cacctgccgg gacatggcac
      421 ccagggtaag ggatgagcgg acgcgcgtca ccggctcgtc cagatcctga catcgagatc
      481 tcgccggacg gacgtcacgg gatggcctcg ccgagcgcgg cgaccacgtc ggccttgcgc
      541 gggaggccga ccgcgcgccg gaccacccgg ccagatccgt cgaggacgaa gacggtgggg
      601 gtacgcatga cgtccagccg ccgtacgagg tccaggtggg cctcggcgtc gacctccagg
      661 tgggcgacgc cctccacctg ctcggccacc tcgccgagga tccgccgcgt cgcacggcac
      721 ggctggcaga acgcggtgga gaactggacg agggtcgccc ggcgcccgag ggcgccgccg
      781 agctcggccg cggtcagcga agggacggcg tggttctcgt ctgcggtgct cgaggtcgag
      841 cgcgcgtcta cgccccggat ccggccgtcg gtgagccgtc gccacacgcc gaaccccgtc
      901 gccgcgacga ggacgacgac cagcagcacg agcccggggt tcacgtacgc cgcaacccac
      961 gcacggccgc ctggcattcc cgccggccca taccgggccg ggttggcccg cctgccggcc
     1021 aaccggcgtc cgtctcggcg acagtgggcc agacggacgc ggtgagcgac acggtcagcc
     1081 gcccgcgaag gggggcaacg cctcgacgac ggcgccgtcc gagagcgcga ccgagtcggg
     1141 cggacgggta ccgaccgggt caccgtcgac gaggaacgcg caccgcccga ggacccgagc
     1201 cagccgcccg tcgtccgggt gggcggcgac ggcagcggcc agcagctcgg ccacggtcgc
     1261 cgggccggcg aaggcatcct cggccaggcc ggcggcttcc ttcgccgccg cccagtaccg
     1321 caaggtcacc gtcacgcacc cacctgcccg cgcttcaccc ggcgaacggg tgctgcagcg
     1381 ccgccgaaag cctctggcac gccgggcgaa cccccaccgg ccgggtgaag cggcgtggcc
     1441 gaggccacca cggcgcgcac ccacgcggcg atccggcggg tgagctcgtc gtcgatggct
     1501 gcctccgcgt ggccgaagcc ggactcgacc cacagctcgg tcgtgcgtcc accagccgcg
     1561 gcgagctcct cggcgtgctc gagggggaag aaccggtcct ggtcgccgtg gacgacgagc
     1621 agcggcgtcg gcgcgacgtc gccggcgacg gctcgcggct cggcgggtac ggggtcccag
     1681 ccctcgtcat ggatccgggt acccatcgcg acccgtgcga agaggcgacc gatcgggtgc
     1741 tcgaccagga agtgcagccg tcgcatcgtg gccgtccctc ggtagtacca gcgggccggg
     1801 ccgctgaccg cgacgaccgc gtcgaccccg cggtagagcg ccgcgtgccg tatgacgacc
     1861 gacgccccca tcgagaaccc caccgtggcc acggaccggt acccgaggag gcgcgcccag
     1921 cggacggccg cgtcgaggtc cagcacctcg aggtcgccga gggtgctgta cccctgcgac
     1981 cggccgtgcc cgcggaagtc gaacgcgacc actccggccc ggtcggagag gacccgagtg
     2041 accctggtga gcgacga
//
LOCUS       DATCCH010000060         2519 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_90366, whole genome shotgun sequence.
ACCESSION   DATCCH010000060 DATCCH010000000
VERSION     DATCCH010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90366"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /gene="ispH"
                     /locus_tag="VIJ44_01245"
     CDS             complement(<1..815)
                     /gene="ispH"
                     /locus_tag="VIJ44_01245"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011438255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEY5164552.1"
                     /translation="MRRMAPTRLVLASPRGYCAGVDRAVDTVERALDHFGPPVYVRRE
                     IVHNRHVVAELTGRGAVFVESEEDVPTGAVLVLSAHGVAPEVHRRSAERELRVLDATC
                     PLVTKVHAEARRFAERGYTIVLVGHAGHEEVVGTMGEAPEAIRLVESIADVDMLEVPD
                     PMRVAYITQTTLSVDETVEILAALRRRYPAAVGPKRDDICYATQNRQNAVKRLANEVD
                     LVLVIGSQNSSNSNRLVEVTREFGVASHLIDDERDIDPRWLEGITTVGITSGAS"
     gene            complement(864..1355)
                     /locus_tag="VIJ44_01250"
     CDS             complement(864..1355)
                     /locus_tag="VIJ44_01250"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEY5164553.1"
                     /translation="MSRRLTIFLVVGLLAGGVGATTARGAEAHWYPLFQAINRARAKH
                     HLRPVAASPRLHWAARRHSSDMLAHDYFGHTSPSGGTLYDRIVRSGFLTEGTWWAGET
                     LAWGTGTTGDPDSVVHMWLESPEHRAILLSPLYRFVGIGRARGTFLRHPDANVWTADW
                     GHR"
     gene            complement(1401..>2519)
                     /locus_tag="VIJ44_01255"
     CDS             complement(1401..>2519)
                     /locus_tag="VIJ44_01255"
                     /inference="COORDINATES: protein motif:HMM:NF015588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HEY5164554.1"
                     /translation="LSGAWFAWESAASGEDVTPAYVTGPGGRRLRVLTGEREIHVVAD
                     IAWAVDTYVRWSGDDRFLDEGGAELTVQAARFFATRAVETSRGFEILHVIGPDELHED
                     VSNSAFTNTMAAWTLQRAAELADAGRAKSQPGEPARWREIGDRMLLLHTPDGLIEQHE
                     GFLDLPLAPDERAGHDELAWQRDRQEWRDVKQADVVMLMSLLEPQFTPEQRLGNFRLY
                     EPLTRHLSSLSEAVHSVVARRLGLHGEADDYLRRAIAIDLDDSRSNRASGLHMATQGG
                     LWQAVAIGCAGVRAGETALEFDPRLPPGWRLLRFRVAHRGVPLIVTLTPDQIEIEART
                     AVARVRLGALEAEVRAGTPLRITRTAGEWHLPAGFTDS"
BASE COUNT          363 a          891 c          894 g          371 t
ORIGIN      
        1 gaggcccccg acgtgattcc caccgttgtg atgccctcga gccagcgtgg gtcgatgtcg
       61 cgctcgtcgt cgatcaggtg cgacgcgacc ccgaactcgc gggtcacctc gaccagccgg
      121 ttggagttgg acgagttctg ggagccgatc acgagcacca gatcgacctc gttcgcaagc
      181 cgcttcaccg cgttctggcg gttctgggtg gcgtagcaga tgtcgtcgcg cttgggccct
      241 acggcggccg ggtagcggcg ccgcagagcc gcgaggatct cgaccgtctc gtccacggag
      301 agggtcgtct gcgtgatgta ggccacccgc atcgggtcgg gcacctcgag catatcgacg
      361 tcggcgatcg actcgaccag ccggatcgct tcgggcgcct ctcccatggt gccgacgacc
      421 tcctcgtgcc cggcgtggcc gacgagcacg atggtgtagc cgcgctcggc gaaccggcgc
      481 gcctcggcgt gcaccttcgt caccagcgga cacgtcgcgt cgaggacccg cagctcgcgc
      541 tcggcactgc ggcggtgcac ctcgggcgcg actccgtgcg ccgagaggac gagcacggcg
      601 ccggtgggga cgtcctcctc ggactcgacg aagacggcac cgcggccggt cagctcggcg
      661 accacatggc ggttgtgcac aatttcgcga cgcacgtaca cgggcggccc gaaatggtcg
      721 agcgcccgct cgacggtgtc aacggcccgg tcgactccgg cgcagtagcc gcgcggagag
      781 gccaggacga gtctggtcgg tgccatgcgc ctcatgctac cgcccaacgg ccgcggcggt
      841 cgccgtgccc gcgccgcctg cgcctagcga tgaccccagt cggccgtcca cacgttggcg
      901 tcggggtggc gcaggaatgt gcctcgggcc cggccgatgc cgacgaaccg gtacaggggc
      961 gacagcagga tcgcccggtg ctcggggctc tcgagccaca tgtgcacgac cgagtcgggg
     1021 tcgcccgtcg tgcctgtgcc ccaggccagc gtctcgccgg cccaccacgt cccctccgtc
     1081 aggaagcccg agcggacgat gcggtcgtag agggtgccgc cgctggggct ggtgtggccg
     1141 aagtagtcgt gggcgagcat gtcgctcgag tgccggcggg ccgcccagtg caggcgcggc
     1201 gacgccgcaa cgggacggag gtggtgtttg gcacgagcgc ggttgatggc ctgaaacagc
     1261 ggataccagt gtgcctccgc gcctcgggcg gtcgtggcgc ccacgccgcc tgcgagcaga
     1321 ccgactacga ggaagatggt cagtcgacgg ctcataccat tggtgatcga cctgcgaaag
     1381 gcctggatta gccggattcg tcaagaatct gtaaaaccgg cgggcagatg ccactcgccc
     1441 gccgtgcgcg tgatccgcag cggcgtgccg gcgcgcacct ccgcctcgag cgcgccgagg
     1501 cggacgcggg cgacggccgt gcgcgcctcg atctcgatct ggtcgggcgt cagggtgacg
     1561 atcagcggca ccccgcgatg cgcgacccgg aagcgcagca ggcgccagcc cggtgggagg
     1621 cgtgggtcaa actcgagtgc tgtctcgccg gctcggacgc cggcgcatcc gatcgccacc
     1681 gcctgccaca gaccaccctg ggtcgccatg tgcaggccgg aggcgcgatt gctgcgggag
     1741 tcgtcgaggt cgatcgcgat cgcgcgccgc aggtagtcgt cggcctcccc gtgcaggccg
     1801 agcctgcgcg cgacaaccga gtggacggcc tcgctgagcg acgataggtg cctcgtcagc
     1861 ggctcgtaca gccggaagtt cccgagccgc tgctccggcg tgaactgcgg ctcgagcaac
     1921 gacatcagca tgacgacgtc ggcctgtttg acgtcgcgcc actcctgccg gtcgcgctgc
     1981 cacgccagct cgtcgtggcc tgcgcgctcg tcgggtgcga gcggtaggtc gaggaagccc
     2041 tcgtgctgct cgatcaggcc gtccggcgtg tgcagcaaca gcatccggtc gccgatctcg
     2101 cgccagcggg ccggctcgcc cggctgcgac ttcgcccgtc cggcgtcggc cagttcggcc
     2161 gcgcgttgca gcgtccacgc cgccatggtg ttcgtgaagg cgctgttcga gacgtcctcg
     2221 tgcagctcgt cgggaccgat cacatggagg atctcgaacc cgcgcgaagt ctcgaccgcc
     2281 ctggtcgcga agaagcgggc ggcctgcaca gtgagctcgg ctccgccctc gtcgagaaag
     2341 cggtcgtcgc cgctccagcg cacgtacgtg tcgaccgccc acgcgatgtc ggccacgacg
     2401 tggatctccc gctcgcccgt cagcacccgc agccggcggc cgcccggacc ggtcacgtac
     2461 gccggcgtca cgtcctcgcc cgatgcggcc gactcccagg cgaaccacgc cccggaaag
//
LOCUS       DATCCH010000061         3847 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_335618, whole genome shotgun sequence.
ACCESSION   DATCCH010000061 DATCCH010000000
VERSION     DATCCH010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3847
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335618"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="VIJ44_01260"
     CDS             complement(<1..645)
                     /locus_tag="VIJ44_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002627761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase-like domain-containing protein"
                     /protein_id="HEY5164555.1"
                     /translation="MPSRIEQYALIGDTQTAGLVADDGSLDWLCAPRFDSPACFAALV
                     GDDSHGRWLLGPAAGGRAARRQYREGTLVLETEFETPEGAVRIIDFMPLRDETIDVIR
                     IVEGIRGAVPMRMDLVIRFDYGSIVPWMHRHRGALHAIGGPDALVLHTPVETRGEDMR
                     TVAEFTVRARERIPFVLAYLPSTAPFPEPADAEAALVRTSQWWRRWSARSTYQGE"
     gene            complement(659..1075)
                     /locus_tag="VIJ44_01265"
     CDS             complement(659..1075)
                     /locus_tag="VIJ44_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164556.1"
                     /translation="MSGLWTPSGEHEPGAGPGGDDDPSGPPGGGPPGGPVEEAELAAE
                     MHRVRAELAATPVEDLVANHAVGLWQLAVLHLVPDGDEPPRLDHAAVAIDAFAAIVET
                     LGPRLGPHEEPLRDALGQLRMAFVQVQERVSGGSGP"
     gene            1248..1952
                     /gene="infC"
                     /locus_tag="VIJ44_01270"
     CDS             1248..1952
                     /gene="infC"
                     /locus_tag="VIJ44_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014983430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEY5164557.1"
                     /translation="MLPASATVRVRQPLRCDISTDDTRINDRIRAREVLLIGGEGEQL
                     GVKPLPEAISIARAAELDLVEVAPNANPPVCRLMDYKRYEYEQQQRRKESRRKATQVV
                     IKEMKFRPKIDVHDYTTKMRHVERFLAEGNKVKLTIMFRGREMAHPELGLRILERIAD
                     EVNEIAMVESAPRQDGRNMTMVLHPLKQNNRRPSTPSKAAKPAATTAETTAPATSPPP
                     APAEPAPSTTAPAQEH"
     gene            1952..2149
                     /gene="rpmI"
                     /locus_tag="VIJ44_01275"
     CDS             1952..2149
                     /gene="rpmI"
                     /locus_tag="VIJ44_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004006287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEY5164558.1"
                     /translation="MPKHKTHRGAAKRFKITGRGKIRRRQAYRSHLLEKKSSRRTRRL
                     GREVEVTGGQKKHVERLLGRA"
     gene            2188..2550
                     /gene="rplT"
                     /locus_tag="VIJ44_01280"
     CDS             2188..2550
                     /gene="rplT"
                     /locus_tag="VIJ44_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020459340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEY5164559.1"
                     /translation="MARGKRSVHSKKRRRRILASAEGYSGARSRHLKTANEQVLHSGQ
                     YAYRDRRARKGEFRRLWIVRINAACRDYEISYSRFVAGLKAAEIDVDRKILADLAVRD
                     SAAFGALVTQARTALEAA"
     gene            2547..3365
                     /locus_tag="VIJ44_01285"
     CDS             2547..3365
                     /locus_tag="VIJ44_01285"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEY5164560.1"
                     /translation="MTNRGLGARHADVQRLRVLARDRHARHTEHAFLLEGPRLLDTAL
                     ERGVPLEAVYLGYGARPAFTTLVDRAVAAGIPVRDLREGVLEKVGTTRTPQPVLAVAP
                     LAPEPAALPTHGDILVAVDVADPGNLGTIIRSAEAAGATAVVVTGASVDPHNPKVVRS
                     SAGAIFGLSIVEQPDPAAALDALHDHGRHTVGTAAIGGHACDTLEIATPIALVVGNEA
                     HGLAPEVRSRLDEIVTIPMHGTGNSLNVAMAATVLLFDLDRRRTRTTPSTGPAR"
     gene            3362..>3847
                     /locus_tag="VIJ44_01290"
     CDS             3362..>3847
                     /locus_tag="VIJ44_01290"
                     /inference="COORDINATES: protein motif:HMM:NF013568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEY5164561.1"
                     /translation="MSLADEQARLDAALSEARATIPDAVSTAALAELERDFAGKRSVA
                     SAVNEAIKSLPAPERPVAGQAVQAYRAAIAELAGHRRETLAAEEAATAAVTERIDLTL
                     GTHTRPRGHLHLVTQIRRELEDIFTGLGYRVAEGPEVEDDWHNFEALNIPPAHPARSM
                     QD"
BASE COUNT          673 a         1574 c         1144 g          456 t
ORIGIN      
        1 ctcaccttgg taggtcgaac gcgccgacca ccgccgccac cactgactgg tccgcaccaa
       61 cgcggcctcc gcatccgccg gctcggggaa cggggccgtg gacggcaggt acgccaacac
      121 gaacgggatc cgctcccggg cccggacggt gaactcggcc acagtcctca tgtcctcacc
      181 acgggtctca accggggtgt gcaacaccaa cgcatccggg ccgccaatgg cgtgcagcgc
      241 accccgatgt cggtgcatcc acgggacgat cgacccataa tcgaaccgga tcaccagatc
      301 catgcgcatc ggcaccgcac cccggatccc ctccacgatg cggatcacgt cgatcgtctc
      361 gtcccgcaac ggcatgaagt cgatgatccg gaccgcccct tccggggtct cgaactcggt
      421 ctcgagcacc aacgtcccct cccgatactg acgccgcgcc gcgcgcccac ccgcggccgg
      481 ccccagcagc caccgaccgt gcgagtcatc cccgaccagc gcggcgaaac acgccggaga
      541 atcgaaccgg ggcgcgcaca gccagtcgag cgacccgtcg tcggcgacga gccccgcggt
      601 ctgggtgtca ccgatgagcg cgtactgctc gatgcgcgaa ggcatgcgag cacggatgct
      661 aggggccaga accgcccgaa acgcgttcct gcacctgcac gaacgccatc cgcagctgac
      721 ctaacgcgtc ccgcagcggc tcctcgtggg gacccaaccg gggccccaac gtctccacga
      781 tcgccgcgaa cgcgtcgatc gccaccgccg cgtggtcgag ccggggcggc tcgtcaccat
      841 cggggacgag gtgcagcacc gcgagctgcc acagcccgac agcatggttg gcgaccaagt
      901 cctcgaccgg cgtcgccgcc agttcggccc ggacccggtg catctcagcc gcgagctcgg
      961 cctcctcgac cggcccgccg ggcggtccgc cgcctggggg cccactcggg tcgtcgtcgc
     1021 cacccgggcc cgccccgggc tcgtgctcac cggaaggtgt ccataagccg ctcacactgg
     1081 tatcctcgtc gctcgacaac cgatgtggga agtgggacga ccttccgccg ccagccaaag
     1141 ggcgagagac cgtccccacc cggccgggac ccgtacccac gggccgctcc gagcccgggt
     1201 ccgtcctttg agccggggcg tgttcgcccc gaccctggtg gtggcggatg cttcctgcgt
     1261 ccgccactgt tcgcgtccgg caaccattga ggtgtgacat aagcacagac gacaccagga
     1321 tcaacgaccg catccgagcc cgcgaagtgc tgctcatcgg cggcgaaggc gaacagctcg
     1381 gcgtcaagcc gctccccgag gcgatctcca tcgcccgggc cgcggaactc gacctcgtgg
     1441 aagttgctcc gaacgcgaac ccgccggtct gccggctcat ggactacaag cgctacgagt
     1501 acgagcagca gcagcggcgc aaagagtccc gccgcaaggc cacccaggtc gtcatcaagg
     1561 agatgaagtt ccgaccgaag atcgacgtgc acgactacac gaccaagatg cgccacgtcg
     1621 agcggttcct cgccgaaggc aacaaggtca agctcaccat catgttccgg ggccgagaga
     1681 tggcccaccc cgagctcggc cttcgcatcc tggaacgcat cgccgacgag gtcaacgaga
     1741 tcgcgatggt ggagtcggcc ccccgccaag acggacgcaa catgaccatg gtcctgcacc
     1801 ccctcaaaca gaacaaccgc cgccccagca cccccagcaa ggctgccaaa ccggccgcga
     1861 ccaccgctga gaccacggcc ccagccacca gcccgccacc agcccccgcc gaaccggcac
     1921 cgtccaccac cgccccagct caggagcact gatgccgaag cacaagacgc atcgcggcgc
     1981 cgcgaaacgc ttcaagatca ctggccgcgg caagatccgg cgccgccagg cgtaccggtc
     2041 ccacctcctc gagaagaagt cgagtcgccg cacccggcgc ctcggacgcg aggtcgaggt
     2101 caccggcggc cagaagaagc acgtggagcg cctcctcggg cgcgcgtaac cgcccctcgg
     2161 agccaccgat caccaacggg agcagtcatg gcacgcggca agcgttccgt ccattccaag
     2221 aagcgccgcc ggcggatcct cgcgtccgcc gagggctact ccggcgcgcg cagccgccac
     2281 ctcaagaccg cgaacgaaca ggtcctgcac tccgggcagt acgcgtaccg cgaccgccgg
     2341 gcccgcaagg gcgagttccg tcgcctctgg atcgtccgca tcaacgctgc gtgccgcgac
     2401 tacgagatct cgtactcccg gttcgtcgcc ggcctcaaag ccgccgaaat cgacgtcgac
     2461 cgcaagatcc tcgccgacct cgcggtgcgc gactccgcgg cgttcggtgc cctcgtcacc
     2521 caggcccgca ccgcgctcga agcggcttga cgaaccgcgg gctcggcgcc cgccacgccg
     2581 acgtccagcg cctccgggtc ctggcccgcg accgacacgc gcgccacacc gaacacgcct
     2641 tcctgctcga aggcccccgc ctcctcgaca cggccctgga acgcggcgta ccgctcgaag
     2701 ccgtctacct cggctacggc gcccgacccg cgttcacgac cctcgtcgac cgagccgtcg
     2761 ccgccggcat ccccgtccgg gacctgcgcg aaggcgtcct cgagaaagtc ggaacgaccc
     2821 gcacccccca gccggtcctc gccgtcgccc ccctggcccc cgaaccggcg gcgcttccca
     2881 cccacggcga catcctcgtc gcggtcgacg tcgccgaccc cgggaatctc ggcaccatca
     2941 tccgcagcgc cgaagcggcc ggcgccaccg ccgtcgtcgt caccggcgcc tcggtcgacc
     3001 cccacaaccc caaggtggtg cgctcgtccg cgggtgcgat cttcgggctc tcgatcgtcg
     3061 agcagcccga cccggccgcc gcgctcgacg cgctccacga ccacggccgt cacaccgtcg
     3121 gcaccgccgc gatcggtggc cacgcctgcg acaccctcga gatcgccacc ccgatcgccc
     3181 tcgtcgtcgg caacgaggcc cacgggctcg cccccgaggt ccggtcccgc ctcgacgaga
     3241 tcgtcaccat cccgatgcac ggcaccggca actccctcaa cgtcgcgatg gccgccactg
     3301 tcctgctctt cgacctcgac cggcgccgca cccggaccac accctcgacg ggaccagccc
     3361 ggtgagcctc gccgacgaac aggcccgcct cgacgccgcg ctcagcgaag cccgcgccac
     3421 gatccccgac gccgtctcga ccgcggcgct cgccgagctc gaacgcgact tcgcggggaa
     3481 acggtccgtg gccagcgccg tcaacgaggc catcaagtcg ctccccgcac ccgagcggcc
     3541 cgtcgcgggt caagccgtcc aggcgtaccg cgccgcgatc gccgagctcg ccgggcaccg
     3601 ccgggagacg ctcgccgccg aggaggccgc caccgccgcg gtcaccgaac ggatcgacct
     3661 gacgctcggc acccacaccc gaccccgagg tcacctgcac ctcgtcaccc agatccgccg
     3721 cgaactcgag gacatcttca ccgggctcgg ctaccgcgtc gccgaaggac ccgaagtcga
     3781 agacgactgg cacaacttcg aagccctcaa catcccaccc gcgcacccgg cccgctccat
     3841 gcaagac
//
LOCUS       DATCCH010000062         2314 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_463972, whole genome shotgun sequence.
ACCESSION   DATCCH010000062 DATCCH010000000
VERSION     DATCCH010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463972"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="VIJ44_01295"
     CDS             complement(<1..525)
                     /locus_tag="VIJ44_01295"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5164562.1"
                     /translation="MKDVVVIGAGMAGVTAARDCARAGLDVVVVEAQDRIGGRMFTAR
                     DFCDYPVEQGAEFVHTAEADTWPEIEDAGLETVRCDPASGFVLSVGGVQSPKLFKDPS
                     LVRLGDLLQDIVDYDGPERTVAEFLAARQLDGPARTLADQMLTLHPLGDQDQLSVIGL
                     RDDRVVDLERGCDWR"
     gene            555..1349
                     /locus_tag="VIJ44_01300"
     CDS             555..1349
                     /locus_tag="VIJ44_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020788546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5164563.1"
                     /translation="MRIEGSTILVSGASSGIGAALAPQLAARGATVGIVARRRDRLEA
                     VLERCTQHAPRSRSWTVDLGDLDAAVRVVDEAWDAFTGLDCLVNNAAIPKRVPVPRLT
                     DSLIDETMRINFTSPVRMTMALVPRWIQRGSGCVVNVSSMGGRIGIAHEAAYCASKFA
                     LCGWSEAMAIDLRGTGVDVKLALPGPIETEIWDLPGNDAALYNGPFVSAETCAASIVD
                     AIEGDGFEFFVPPEFPGGLGAQHDMVVGKTRDVDAFIDLMGQMAGS"
     gene            complement(1355..>2314)
                     /locus_tag="VIJ44_01305"
     CDS             complement(1355..>2314)
                     /locus_tag="VIJ44_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEY5164564.1"
                     /translation="ETTRHADVILPAPPLLARGHYDLALYSLAVRNVANYSPPLVALE
                     AGEMHEWEILLRLANIAAGHGAAADPHALDDVIVAGMVDKAVRRGASNVAGRDPADLL
                     EELAARRGPERILDLMLRTGPYGDGFGVDRDGLSLAVLEDHPHGVDFGPLTSRVPEML
                     RTPSGRIELAPDPIAADVDRLRSTLRGASAPGLTLIGRRDLRSNNSWMHNVEVLVKGR
                     DRCTLLVHPDDAAQLGLRDGGRAKVVAVGGSLFAPVEVSDTMMRGVVSLPHGWGHDVD
                     GVQLRVASSRPGVNSNLLADTEHFDPLSGNAVLNGIPVDVAPA"
BASE COUNT          371 a          809 c          787 g          347 t
ORIGIN      
        1 ccgccagtca cagccccgct ccagatcgac gacccggtca tcgcgcaagc cgatcaccga
       61 cagctggtct tggtcgccga ggggatgcaa ggtgagcatc tgatcggcga gggtccgggc
      121 tggcccgtcg agttggcgcg ccgcgaggaa ctccgcgacc gtgcgctccg ggccgtcgta
      181 gtcgacgatg tcttggagca gatccccgag ccgcacgagc gacgggtctt tgaacagctt
      241 gggggactgc acgcccccga cggagagcac gaaccccgac gcggggtcgc atcgcaccgt
      301 ctcgagtccc gcgtcctcga tctcaggcca ggtgtccgcc tcggcggtgt ggacgaactc
      361 cgcgccctgc tccaccggat agtcacagaa atcacgcgcc gtgaacatcc ggccgccgat
      421 tcggtcctgg gcttcgacga ccaccacgtc gagaccggcg cgcgcacagt cgcgagccgc
      481 ggtcacaccg gccatcccgg ccccgataac gacgacgtct ttcatcaggg cgggatggta
      541 ccgtcgccgc ccgcatgcgc atcgagggct cgaccatcct ggtatccggg gcgtcgtccg
      601 gcatcggcgc ggcgctcgca ccgcagctcg cagcgcgagg cgcgacggtc gggatcgtcg
      661 cccggcgtcg cgaccgcctc gaagcggtgc tggaacgctg tacccagcac gctccgcgat
      721 cgcgatcgtg gacggtcgac ctcggtgatc tcgacgccgc cgtccgtgtc gtcgacgagg
      781 cctgggacgc cttcacgggc ctggactgcc tcgtcaacaa cgcggcgatc ccgaagcggg
      841 tgcccgtccc acgactgacg gactcgctga tcgacgagac gatgcggatc aacttcacgt
      901 cgccggtccg tatgaccatg gcgctggtcc cgcgctggat ccaacgtggc tcgggctgcg
      961 tcgtgaacgt gtcgagcatg ggtgggcgaa tcgggatcgc ccacgaggct gcctactgcg
     1021 cctccaagtt cgcgctgtgt ggctggagcg aagcgatggc catcgaccta cgcgggactg
     1081 gcgtcgacgt gaagctcgcg ctgccgggtc cgatcgagac cgagatctgg gacctacccg
     1141 gcaatgacgc cgctctctac aacggcccgt tcgtctccgc cgagacgtgc gcggcgagca
     1201 tcgtcgacgc gatcgagggg gacggattcg agttcttcgt ccctccggag ttccccggtg
     1261 gactcggcgc gcagcacgac atggtggtcg gcaagacccg cgacgtcgac gccttcatcg
     1321 acctcatggg tcagatggct ggtagctgag acgactacgc gggggcgacg tcgaccggga
     1381 tcccgttgag caccgcgttg cccgaaaggg gatcgaaatg ctcggtgtcg gcaagcaggt
     1441 tgctgttcac acccggccgg ctcgacgcca cccgaagctg cacgccgtcg acgtcgtgac
     1501 cccacccgtg cggcaggctg acgacacctc gcatcatcgt gtcgctcacc tcgaccgggg
     1561 cgaagagcga tccgccgacg gccacaacct tggctcgccc cccgtcgcgc aagccaagct
     1621 gagccgcgtc gtcaggatgg acgagcaacg tgcaccggtc gcggcccttg accaggacct
     1681 caacgttgtg catccaggag ttgttggagc ggaggtcgcg ccggccgatc agcgtgagcc
     1741 caggcgccga cgctccacgg agcgtcgatc gcagccggtc aacgtcggcg gcgatcggat
     1801 cgggcgcaag ctcgatccgg ccactcggcg tgcgcagcat ctcgggcacc cgtgaggtca
     1861 acggtccgaa gtcgacgccg tgggggtggt cctcgagcac ggcgagcgac aagccgtcgc
     1921 ggtccacccc gaatccgtcg ccgtacggcc cggtccggag catcagatcc aggatccgtt
     1981 ccggcccgcg ccgcgccgcc agctcctcga gcaggtcggc cgggtcacgg ccagccacgt
     2041 tcgaagcccc tcgacggacc gccttgtcca ccatgccggc cacgattacg tcatcgaggg
     2101 cgtgcggatc cgcggcagcc ccgtggccgg ccgcgatgtt ggcgagacga aggaggatct
     2161 cccactcgtg catctccccc gcttcgaggg ccacgagcgg gggtgagtag ttcgccacgt
     2221 tgcgcaccgc gagcgagtac aaggccaggt cgtagtggcc ccgggccagg agcggcggtg
     2281 caggcaggat cacgtccgcg tggcgagtgg tctc
//
LOCUS       DATCCH010000063         2612 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_256188, whole genome shotgun sequence.
ACCESSION   DATCCH010000063 DATCCH010000000
VERSION     DATCCH010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256188"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1000)
                     /locus_tag="VIJ44_01310"
     CDS             complement(<1..1000)
                     /locus_tag="VIJ44_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164565.1"
                     /translation="MGLFDGLLNGLGWIWDQTAGRATSAVWDQIVNGLVSWVVNSITW
                     FVSALLQFFERSSTPTLASNWFAGGPIGTNAHSPYGVVASLALSVLVLCVLISVVHGL
                     LTGEGPAMIGRIARDVPLSVLGIVATIGVAQVLLGAADELAARILVGTQAGAHATAVL
                     HAVGQVQASSGQPTFVVFLLGLVAVLGAFVLWVELLVRASLLYVLLALSPFAYAAFVW
                     PSARRILHRLVELIVALVFSKVVIAVTLAVAASALASSNAGGLATGEAKIGTLLVGTI
                     MFLLAAFSPFLLLRLFPVVEAAVVAHGISRAPARATQRAMFTALTVTRLAGGLSGTG"
     gene            complement(1000..1677)
                     /locus_tag="VIJ44_01315"
     CDS             complement(1000..1677)
                     /locus_tag="VIJ44_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164566.1"
                     /translation="MATVTLGTRTLVLALAATAITGMGARGLIASPQTPPRTTAATAA
                     KTPGPRSTTSGMPTGFARNEPGAIAAATSYVRQGQHLLDLPTADRTPALQVIASLDGA
                     NGYVAQVTAELAELDGIEARGQGHLTWDVSVLATRVDAYNAQRARISIWRVGVLSVTG
                     LVAPLAEWTTVDDELVWERGDWKVWAETQTPGPTPAGQPDARPSTPTQLHSALAGYTR
                     YPGLDPL"
     gene            complement(1679..1987)
                     /locus_tag="VIJ44_01320"
     CDS             complement(1679..1987)
                     /locus_tag="VIJ44_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164567.1"
                     /translation="MGALAAVARRLVADGVNATPSANGLPGAGFVQRLIDWTAQIGLW
                     GSLAALLVGAAMFGLSQQAGNYAGASRGKHLVMGGAIGAAITGLAPTIVNLVFTASRS
                     "
     gene            complement(2073..>2612)
                     /locus_tag="VIJ44_01325"
     CDS             complement(2073..>2612)
                     /locus_tag="VIJ44_01325"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial transcriptional activator
                     domain-containing protein"
                     /protein_id="HEY5164568.1"
                     /translation="FHRHVGRLDASVQTDVDTLVSARSRAEYQATTDAAATLADALEL
                     VRGRPFDVATGYEWAFTELHVAHAERVVVDVAHDLAGLALTLGDWRRTLWATDVGLRA
                     CPASELLYQDRMRACAAGSDRRGVESAMRDLRRTLATTDSSDVPSRDTFELYEHLHTR
                     LQRGTPTDPPVATRNLPSR"
BASE COUNT          406 a          809 c         1034 g          363 t
ORIGIN      
        1 cccccgtccc gctcaagccg ccggcgagtc gagtgaccgt gagcgcggtg aacatcgccc
       61 gctgcgtcgc acgagctggg gcgcgcgaga tgccatgggc gacgaccgcg gcctcgacga
      121 cgggaaagag tcggagcagc aagaacggcg agaacgccgc gaggaggaac atgatcgtcc
      181 cgacgagcag cgtgccgatc tttgcctccc cggtggcgag accgccggcg ttggagctgg
      241 ccagcgccga ggcggcgacg gcgagcgtga ccgcgatgac gaccttggag aagacgagag
      301 cgacgatcag ctcgacgagt cggtgcagga tccgacgtgc gctgggccag acgaaggccg
      361 cgtaggcgaa tgggctgagt gcgagcagca cgtagaggag ggaggcacgc acgaggagct
      421 ccacccagag gacgaaggcg ccgaggacgg cgacgagccc gagcaggaac accacgaagg
      481 tcggctgtcc cgacgacgct tggacctggc cgacggcgtg caggaccgcg gtggcatggg
      541 cgcccgcctg ggtgccgacg aggatccggg cggcgagctc gtcggcggca ccgagcagga
      601 cttgcgccac gccgatggtg gcgacgatgc cgaggaccga gagtggaacg tcgcgagcga
      661 ttcggccgat catcgcgggg ccctcaccgg tgagcagccc gtggacgacg ctgatgagga
      721 cgcagagcac cagcacgctg agggccaggc tggcgacgac cccgtacggg gagtgcgcgt
      781 tggtgccgat gggtccgccg gcgaaccagt tgctggcgag cgtgggcgtg gatgagcgct
      841 cgaagaactg gagcagagcg gagacgaacc aggtgatcga gttgacgacc cagctcacga
      901 gaccgttgac gatctggtcc cagacggcgc tggtcgctcg tccggcggtc tgatcccaga
      961 tccagccgag cccgttgagg agcccgtcga agagacccat cagagcgggt cgaggccggg
     1021 gtagcgggtg tagccggcga gcgcgctgtg gagctgggtg ggggtcgacg gacgcgcgtc
     1081 gggttggccc gcgggcgtgg gtcccggtgt ctgggtctcg gcccagacct tccagtcgcc
     1141 tcgttcccac accagctcgt cgtcgacggt tgtccactcg gcgagggggg cgacgagtcc
     1201 ggtcaccgac agcacgccga ctcgccagat cgagatgcga gcccgctggg cgttgtaggc
     1261 gtcgacgcgg gtggcgagga cggacacgtc ccaggtgagg tggccttgcc cgcgtgcctc
     1321 gatgccatcg agttccgcga gctcggcggt cacttgcgcg acgtagccgt tcgccccgtc
     1381 gagcgacgcg atgacttgca aggcgggggt gcggtcggcg gtgggtaggt ccaagaggtg
     1441 ctggccttgg cggacgtagg acgtcgcggc cgcgatggcg ccgggttcgt tgcgggcgaa
     1501 tccggtcggc atgcccgagg tcgtggatcg cggcccgggg gtcttcgccg cggtggcggc
     1561 ggtcgtccgc ggtggagttt gtggcgacgc gatgagtccg cgggcaccca tgccggtgat
     1621 cgcggtcgcc gcgagcgcga ggaccagggt tcgggtcccg agcgtgacgg tcgccatgtc
     1681 agcttcggga ggcggtgaag acgaggttga cgatggtcgg ggcgaggccg gtgatggcgg
     1741 cgccgatggc gccgcccatg acgagatgtt tgccccgcga cgcgccggcg tagttgccgg
     1801 cctgttggga gaggccgaac atggcggcgc cgacgagcag ggcggcgagg ctcccccaga
     1861 gcccgatttg ggcggtccag tcgatgagac gctggacgaa gccggcgccc ggcaagccgt
     1921 tcgcgctcgg tgtcgcgttc acgccgtcgg cgacgagccg ccgtgcgacc gctgcgagtg
     1981 ctcccatcgg ttctccttgc cggcttggtg tcggtggcaa caaggcacga aacccagccc
     2041 ggaacccaca cgaagatgtg tcgtggtggg agctatcgag acgggaggtt tcgagtggcg
     2101 acgggcgggt ccgtcggtgt tccgcgctgg aggcgcgtgt ggaggtgctc gtagagctcg
     2161 aaggtgtcgc gactcgggac gtcggacgag tccgtggtcg cgagggttcg acggaggtcg
     2221 cgcatggcac tctcgacccc gcggcggtcg ctccccgcgg cgcacgctcg catccggtcc
     2281 tgatagagca gctcgctggc cggacacgcg cggagcccga cgtcggtggc ccacagcgtc
     2341 cggcgccagt cccccagcgt gagcgcgagc ccggccagat cgtgtgcgac gtcgacgacg
     2401 acgcgttcag cgtgggcaac gtgcagttcg gtgaaggccc actcgtagcc ggtcgccacg
     2461 tcgaacggcc gtccccggac cagctcgagt gcgtccgcga gcgttgccgc ggcgtcggtg
     2521 gtcgcctggt actcggcccg tgaccgggcc gacacgagcg tgtcgacgtc ggtctgcacc
     2581 gaggcgtcaa ggcgaccgac gtggcggtgg aa
//
LOCUS       DATCCH010000064         2350 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_546517, whole genome shotgun sequence.
ACCESSION   DATCCH010000064 DATCCH010000000
VERSION     DATCCH010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546517"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..149
                     /locus_tag="VIJ44_01330"
     CDS             21..149
                     /locus_tag="VIJ44_01330"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5164569.1"
                     /translation="MFPPTGEMAQPEEVATLIAFLASDDAGYISGAVVPIDMGVTA"
     gene            197..709
                     /locus_tag="VIJ44_01335"
     CDS             197..709
                     /locus_tag="VIJ44_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020555537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY5164570.1"
                     /translation="MAEELQAPMGHGMKQRDVIKMTPAEIDDFLQGRRTMNCATINHD
                     GTIHLVAMWYGFLDGCPALETKAKSQKVQNLRRSDRISALVEDGQAYDELRGVELVGR
                     GEIIDDPARMFELGISVFERYQGLKYSDEMRPFVEQMLNKRVVVKIHIDRYVSWDHRK
                     LGLPSAQAAS"
     gene            784..1446
                     /locus_tag="VIJ44_01340"
     CDS             784..1446
                     /locus_tag="VIJ44_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020660059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY5164571.1"
                     /translation="MISAAIGDAASVLNVGAGTGSYEPSEGRVVAAEPSETMLTQRAD
                     RSAPAIRAVGEWLPVRDRAFDVALAVLTIHHWPDPVAGLRELQRVASRQVILHFEPGF
                     PFWFADEYLPPDRTIDVVDEPTVTEVVAALGNARVEPVPVPHDCLDGFFAAYWRRPAA
                     YLDPSVRANISYLARMGDDDLSPGLDRLRRDLASGAWDAEHQDLLARDELDAGYRLIV
                     AG"
     gene            1558..2247
                     /locus_tag="VIJ44_01345"
     CDS             1558..2247
                     /locus_tag="VIJ44_01345"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY5164572.1"
                     /translation="MFSDPAYLDSWRDRALARTLEPARARSVDRLERLVDAARDLANE
                     AGTAAFTVAQVAARAEVSLKAFYRCFAGKDDLLVALLEEDSRLGAAILNGMVDAHRDS
                     PARLHAYVTGVFELLALPGALGYAGLLVREHRRLSDSRPEEMRRALAPMIDVLAAEIT
                     VAVSTGAVESLDPQRQARAVFALVLDGIHEVARGDAEPLDVATFLWRFCWGGLRGAFN
                     DPTPPDPEEQP"
BASE COUNT          363 a          835 c          794 g          358 t
ORIGIN      
        1 acacccgcga ctaccagaag atgttcccgc ccacgggaga gatggcccag cccgaagagg
       61 tcgccaccct gatcgcgttt ctcgcctccg acgacgccgg gtacatctcg ggcgccgtgg
      121 tgcccatcga catgggagtt accgcgtgag ctgcggggcc gagctgcgcc ccgctgggcc
      181 ccggtagcct ctcgccatgg ccgaggaact tcaggccccg atgggtcacg gcatgaaaca
      241 gcgtgacgtg atcaagatga ccccggcgga gatcgacgac ttcctccagg gccgccggac
      301 catgaactgc gccacgatca accacgacgg caccatccac ctcgtggcga tgtggtacgg
      361 gttcctggac ggctgccccg cgctcgagac gaaggccaag agccagaagg tccagaacct
      421 ccgccggagc gatcggatct ccgcgctggt cgaggatggc caggcctacg acgagctccg
      481 aggagtcgag ctcgtcgggc ggggtgagat catcgacgac ccggcccgga tgttcgagct
      541 cggtatcagc gtcttcgagc ggtaccaagg gttgaagtac agcgacgaga tgcgaccctt
      601 cgtggagcag atgctgaaca aacgggtcgt ggtcaagatc cacatcgacc ggtacgtctc
      661 ctgggatcac cgcaagctcg ggctcccgag cgcccaggcg gcgagctgac gcccccacag
      721 ctctacgagc ggatcggagg cgggtacacc cgtcaccgac ggccggatcc tcgggtcgcg
      781 gcgatgatca gcgccgcgat cggtgacgcc gcatcggtgc tcaacgtcgg cgccggcacc
      841 ggctcctatg agccgagcga aggtcgggtc gtcgccgcgg agccatccga gacgatgctc
      901 acccaacgcg ccgaccggtc ggcgccggcg atccgcgcgg tcggggagtg gctgccggtc
      961 cgggaccggg cgttcgacgt cgcgctcgcg gtgctcacca tccaccactg gccggacccg
     1021 gtcgcggggc ttcgtgagct gcagcgggtc gcgtcgcgtc aagtgatcct gcacttcgaa
     1081 ccgggcttcc cgttctggtt cgccgacgag tacctccctc cggatcggac catcgacgtg
     1141 gtggacgagc cgacggtgac cgaggtcgtc gcggcgctcg gtaacgcccg ggtcgagccg
     1201 gtcccggtcc cgcacgactg tctcgacggg ttcttcgccg cgtactggcg gcggccggcg
     1261 gcctacctgg acccatcggt tcgcgccaac atctcctacc tggctcggat gggcgacgac
     1321 gatctcagcc cgggcttgga tcgcctccgg cgcgacctcg cgtccggcgc atgggacgcg
     1381 gagcaccagg atctgctggc acgggacgag ctcgacgccg gctaccggct catcgtcgcc
     1441 ggctgagcgt tccccgctcg gtccgcccca actgccctca gcccagaacc cttggctcgt
     1501 ctcgaatcga gcccccaatt tcgattcgga gagccgccgg cgcgatagtg gagaacaatg
     1561 ttctccgatc cggcatatct tgattcctgg cgggaccggg cgctggcccg aaccctcgag
     1621 ccggcgcggg cccggtcggt cgaccggctc gagcgactcg tcgacgccgc ccgcgacctc
     1681 gccaacgagg cgggcaccgc ggcgttcacg gtcgcccagg tggcggcgcg cgccgaggtt
     1741 tcgctgaagg ccttctatcg ctgcttcgcc ggcaaggacg acctgctcgt cgcgcttctc
     1801 gaggaggaca gtcggctcgg cgcggcgatc ctgaacggga tggtcgacgc gcaccgcgac
     1861 tcgccggccc gcctccacgc ctatgtgacg ggcgtgttcg agctcctcgc actcccgggg
     1921 gccctcgggt atgccgggtt gctcgtccgc gagcaccggc ggctctccga cagccgaccg
     1981 gaggaaatgc ggcgggccct ggcgccgatg atcgatgtgc tcgccgccga gatcacggtc
     2041 gccgtctcca ccggagccgt cgaatcgctc gatcctcagc gacaggcgcg agcggtcttc
     2101 gccctggtgc tcgacgggat ccatgaggtc gcccgcggtg acgccgagcc gctggacgtg
     2161 gccacgttct tgtggcggtt ctgttggggc gggctgcgcg gcgccttcaa cgacccgacg
     2221 ccaccggatc ccgaggagca accgtgaccc acgacaccgc gaccgaagcg accgctccga
     2281 cgctgctgct cttcgatccc aagacggcca acgcgccgcg cgacacctat cggaagctgc
     2341 tcgccgatgg
//
LOCUS       DATCCH010000065         3146 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694 k141_3746,
            whole genome shotgun sequence.
ACCESSION   DATCCH010000065 DATCCH010000000
VERSION     DATCCH010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3746"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="VIJ44_01350"
     CDS             <1..423
                     /locus_tag="VIJ44_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164573.1"
                     /translation="TYFFFAPLVIPIVILAVPIVDTVFSFVRRLVRGQSFSQADREHL
                     HHRLMRLGHGPRRAVVILWLWTALLSGVALIPTYTNRGNALVPLALAALALLLFTYFH
                     PGLRTRRANRAEGGDPSADGVVDFEAHRRAADSGRRRS"
     gene            535..837
                     /locus_tag="VIJ44_01355"
     CDS             535..837
                     /locus_tag="VIJ44_01355"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEY5164574.1"
                     /translation="MSLRVRRRRSIPNASDDAFSYAVAMVIVPVLFGLLGSWIDGRLG
                     THPLFLLALGAFGVACSFASAYYRYEARISHHDQGKPWTRHPRPAEPDRELEHGRP"
     gene            834..1268
                     /locus_tag="VIJ44_01360"
     CDS             834..1268
                     /locus_tag="VIJ44_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164575.1"
                     /translation="MSAPTIPAVDHIESRIARDLARRALLAAPVIMLGVGLWRGVDAA
                     GAVAVALALIVANFLVAAAALGWAARTSPNLLMGVALFGFLARLALLTGIGVGIKALD
                     IVDWPVFCITLIVGYFGLLFWELRSVSLSLASPGLKPDRHWE"
     gene            1265..2056
                     /gene="atpB"
                     /locus_tag="VIJ44_01365"
     CDS             1265..2056
                     /gene="atpB"
                     /locus_tag="VIJ44_01365"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEY5164576.1"
                     /translation="MTGLNPIVANLLPNINEIFQWKGLFGQNTILEFNKTALMAFVST
                     AICIALFWAGSRRRALVPTGIQNVAEYGYEIVEENISIAAMGEDGRSWTPFFATLFFW
                     IFFINIFEVVPGIDFPATARLAIPLFLALLSWATFIITGFVKQGPKYFWHNVNPEGVP
                     FALKFLVIPIEIFSKYLVRPFSLAVRLFANMTAGHVLLSIFAIMTDQLLIARNSGWYQ
                     AAFSPLPFAGLVAFTGFEVLVAVLQAYIFTILTAVYVNESVHAEH"
     gene            2087..2329
                     /locus_tag="VIJ44_01370"
     CDS             2087..2329
                     /locus_tag="VIJ44_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010119249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEY5164577.1"
                     /translation="MHTLITLGATAPNELKNGLASVGYGLAAIGPGIGIGYLVGQSVQ
                     AMARQPEMAGNVRTTMFLGIAFAEALALIGFVLKFI"
     gene            2338..3054
                     /gene="atpF"
                     /locus_tag="VIJ44_01375"
     CDS             2338..3054
                     /gene="atpF"
                     /locus_tag="VIJ44_01375"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEY5164578.1"
                     /translation="MRTRTLLAGGLLAATVLIAVPTPALAADPGTSAGKDLVACVQKA
                     LKNNEAAIAKSQYTGFQNDLDNCRKATSLFTPAAPELIWGSIAFVIVAFALMKFAFPA
                     MKKGLKAREEKIRGDLEAAERAREEAEESRREYERRLSEARTEATHLIEAAHQDAERV
                     RLELLQRAEADATDVRTRAQDDIRLATERAMSDLRTRVTDMSVELAERIVEHNLDRDT
                     QVALVESYIDSIGTGGNGHR"
BASE COUNT          507 a         1142 c          967 g          530 t
ORIGIN      
        1 acctacttct tcttcgcgcc cctcgtcatc ccgatcgtga tcctggccgt cccgatcgtc
       61 gacacggtgt tctcgttcgt gcggcgactc gtccgaggcc agtcgttctc gcaggccgac
      121 cgcgagcacc tccaccaccg cctcatgcgc cttggtcacg gaccgcggcg ggcggtcgtg
      181 atcctgtggc tctggaccgc gctgctctcc ggggtcgcgc tgatcccgac ctacaccaac
      241 cggggcaacg cactcgtccc gctggccctg gccgcgctcg ccctgctcct cttcacctac
      301 ttccacccgg gactacggac gcggcgggcg aaccgcgccg agggtggcga tccgtccgct
      361 gacggggtgg tcgatttcga agcgcaccgg cgggcggcgg attccggacg gcgccggagt
      421 tgacgatcga aggggccctc gatactgtgt gaaaatattc acaagcgcga cccgggccag
      481 gcggagcctg gcgcggcgcc ggcgctggca gcggaacaag gggaaccagc ggcggtgagc
      541 cttcgggtgc ggcggcggcg aagcatcccg aatgcttcgg acgatgcctt cagctacgcc
      601 gtcgccatgg tcatcgtccc ggtgctgttc ggcctgctcg gctcctggat cgacggtcgc
      661 ctgggcaccc acccgctgtt cctcctcgcg ctgggcgcgt tcggcgtggc gtgctcgttc
      721 gcctcggcct actaccgcta cgaggcccgc atctcccacc acgaccaggg caagccctgg
      781 acgcgccacc cccgaccggc cgagcccgac cgtgagctcg agcacggcag accatgagcg
      841 caccgacgat ccccgccgtc gaccacatcg agtcgcgcat cgctcgagac ctggcccgcc
      901 gggccctgct ggcggcaccg gtcatcatgc tgggcgtcgg tctctggcgg ggcgtcgacg
      961 ccgcgggcgc agtggccgtg gcgctggcgc tgatcgtggc caacttcctc gtcgccgcgg
     1021 cggcgctcgg atgggcggcc cgcacgagcc cgaacttgct catgggcgtc gccctcttcg
     1081 ggttcctcgc ccggcttgcc ctcctcaccg gaatcggggt gggcatcaaa gccctcgata
     1141 tcgtcgactg gccggtgttc tgcatcacct tgatcgtcgg gtacttcggg ttgctgttct
     1201 gggaactgcg ctcggtgagc ctgtcgctcg cgtcacccgg cctaaagcct gaccgtcatt
     1261 gggagtgacc ggcttgaacc cgatcgtcgc caacctgctg ccgaacatca acgagatctt
     1321 ccaatggaag ggtctcttcg gccagaacac catcctcgag ttcaacaaga ccgcgctgat
     1381 ggcattcgtc tcgaccgcga tctgcatcgc cctgttctgg gccggcagcc gccgccgggc
     1441 gctcgttccc accggcatcc agaacgtcgc cgagtacggc tacgagatcg tcgaagagaa
     1501 catcagcatc gccgcaatgg gcgaagacgg ccgcagctgg acaccgttct tcgcgaccct
     1561 gttcttctgg atcttcttca tcaacatctt cgaggtcgtg cccggcatcg acttccccgc
     1621 caccgcccgc ctcgcgatcc cgctcttctt ggccctcctg agctgggcga cgttcatcat
     1681 caccggattt gtcaaacagg gcccaaagta cttctggcac aacgtcaacc ccgagggggt
     1741 gccgttcgcg ctgaagttcc tcgtcatacc gatcgagatc ttctccaagt acctggtacg
     1801 cccgttctcg ctcgcagtcc gactgttcgc gaacatgacc gccggccacg tcttgttgtc
     1861 gatcttcgcg atcatgaccg accagctcct gatcgcccgc aacagcggct ggtaccaggc
     1921 cgcgttctcg cccctgccgt tcgcgggcct tgtggcgttc accggcttcg aagtcctcgt
     1981 cgccgttctc caggcctaca tcttcacgat cctcaccgcc gtgtacgtga acgaatccgt
     2041 gcacgccgaa cattgatggc gttacccata tgacaaggag cacagcgtgc acaccctgat
     2101 caccctcggc gccacggcgc cgaacgagct caagaacggt ctcgcgtccg tcgggtacgg
     2161 cctcgcggcg atcggtccgg gcatcggcat cggttacctc gtcgggcagt ccgtccaagc
     2221 gatggcgcgc cagcccgaga tggccgggaa cgtgcgcacc accatgttcc tcggcatcgc
     2281 gttcgctgag gccctggccc tcatcggctt cgtcctcaag ttcatctaag gccgacgatg
     2341 cggacccgaa ccctgctcgc cggcggcctg ctcgcggcca ccgtgctcat cgcggtcccg
     2401 acgccggctc tcgcggccga cccgggcacc agcgcgggca aagacctcgt cgcgtgcgtg
     2461 cagaaggcct tgaagaacaa cgaggcggcc atcgccaaga gccagtacac gggcttccag
     2521 aacgacctcg acaactgccg gaaggcgacc agcctgttca cgcccgcggc gcccgagctc
     2581 atctggggtt cgatcgcctt cgtcatcgtc gctttcgccc tgatgaagtt cgcgttcccg
     2641 gccatgaaga agggactcaa agcccgcgag gagaagatcc gcggcgacct tgaggccgcc
     2701 gaacgcgccc gcgaggaggc cgaggagagc cgccgcgagt acgaacgtcg cctgagcgag
     2761 gcgcgcaccg aggcgaccca cctcattgag gccgcgcacc aggacgcgga gcgggttcgc
     2821 ctagagctgc tccaacgcgc cgaggctgac gccaccgatg tccgcacccg ggcccaggac
     2881 gacatccgcc tcgccaccga gcgcgccatg agcgacctgc gcacccgagt caccgacatg
     2941 tcggtcgagc tcgcggaacg catcgtcgag cacaacctcg accgcgacac ccaggtggcc
     3001 ctcgtcgaga gctacatcga ctcgatcggt accggaggga acgggcaccg atgacgaagg
     3061 cgcgggaacg gaacgaggcg tacgcccaag gcctcctcga cgtcgcgcga ggcgaaggac
     3121 cactcgccga catcgaagac gacctg
//
LOCUS       DATCCH010000066         2541 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_91194, whole genome shotgun sequence.
ACCESSION   DATCCH010000066 DATCCH010000000
VERSION     DATCCH010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91194"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(255..719)
                     /locus_tag="VIJ44_01380"
     CDS             complement(255..719)
                     /locus_tag="VIJ44_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011308010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HEY5164579.1"
                     /translation="MREKMFSIGDDFWIENESGERAFKVNGKALRMRDTLVLEDPSGS
                     ELYKVQEKALRVRDTMQIERDSDTVATVKKALVHVVRDRFAVEVNGGEDLEARGNIVD
                     HEYKIERDGDHVAEVSKRWFRVRDTYGIEIAPDEDNALILAIVVCIDQMSHD"
     gene            1361..2524
                     /locus_tag="VIJ44_01385"
     CDS             1361..2524
                     /locus_tag="VIJ44_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164580.1"
                     /translation="MASWSPVYLKERYTMFAATHIKVRAVLAAAVIGVVIAGVPAVAS
                     ARPFLNRFGTISLLNTTVPATGSAAGDQNPYGVAVVPHSTGDLVRGGVLVSNFNNSQN
                     QQGTGSSIVEISPSGQAHVFAVVPPLTATPAVGLTTALAVLPSGFVVVGSLPAPGGTL
                     DGSAGGLTILDPNGHVVTTLAGGDINGPWDMTAVDQGRNAVLFVTNVLNGTVAAGGAV
                     VDGGTVVRIRLRIHHQHIRVTSNSVIATGFGEHTDPGALVVGPTGVGLGKHDVLYVAD
                     SNANRIAAIPHALTRNHVLGGGGLTISSGGSLNDPLGLAIAPNDHIITVNGADGNAVE
                     TTPSGDQVVTKQLVPNGAGDLFGLAVTPGEDGLYFVNDIGMPDPAANSLELLH"
BASE COUNT          431 a          878 c          798 g          434 t
ORIGIN      
        1 cagctggccg ccgctgacca tcgcagttgc cgttcccttg cgaacgcagt cctctgccct
       61 caaccgcgtt cggcaggcga tcggcttcgg cgaggtaacc cggacggcgg tagcaccatc
      121 gccgtcgacg cgccgacgag aaccaggttc aacgccgccg tcagcgagag gagaagacct
      181 gacccatgac gtatcaggcc agcttaggaa tggcctttgg tggcgtgagg cttgacgggt
      241 ttaggcggtg aggttcagtc gtgggacatc tggtcgatgc agaccacaat ggcgaggatc
      301 agcgcgttgt cctcgtcggg ggcgatctcg atgccgtagg tgtctctgac gcggaaccag
      361 cgcttggaga cctcggcgac gtggtcaccg tcgcgctcga tcttgtactc gtggtcgacg
      421 atgttccccc tggcctcgag gtcctcgccg ccgttgacct cgaccgcgaa gcggtcccgc
      481 acgacgtgga cgagcgcctt cttgaccgtc gccacggtgt cgctgtcgcg ctcgatttgc
      541 atcgtgtccc gcacccggag cgccttctct tggaccttat agagctcgct gccggacggg
      601 tcctccaaga ccagcgtgtc tctcatccgc agcgccttgc cgttgacctt gaacgctcgt
      661 tcacccgact cattctcgat ccagaagtcg tcgccgatcg agaacatctt ctcgcgcatc
      721 cgatacttcg tttgatcagg gccgtggtcg cgtcggagca atggcatgtt cagagaaccg
      781 tagcttcgga gcgcgtggtc cgcatcaccc cggccgggtg atccacggac acgctgagtc
      841 gctgtgtacg agccaccgcc cggctcagac gcgtctgata acacaaaccc gtccgaggct
      901 gggtctgagc ggatcgcata gtcgcggcgc ccaggcgata tccactcgcg gactccaggc
      961 gccccagccg cccgcctcct cgccgaccca caccccaccg ccgttggcga acagcatcac
     1021 cgaacgcgaa gaaagcgcgc gcacgccgca gagcgctccg accaaccgcc caggcccggc
     1081 ggtcgccgcg tccggtcggc gttggggacg agacggcggg cgctgacgcc cgatgccctc
     1141 aacgaccgcc cggagcgttc ggtggtcgat cgaccaccgc gacggagtga gcgctgacaa
     1201 ggccgacgag ccgccggcgt ccgtctcagt cattccaagg ctcgctgcga cggtggctcg
     1261 gaacatgacg ccccgtctga gtggcgctga tgttcgtatc cgcgaccttg cgatttttca
     1321 gatcaggaag gatggcggcg atctggcgtc ttggaagtca gtggccagct ggtcgcccgt
     1381 ctacctcaag gagcgctaca caatgttcgc tgcgacacat atcaaggtcc gggccgttct
     1441 tgcggccgct gtgatcggcg tcgtgatcgc cggggtgccc gctgtcgcct cggcgaggcc
     1501 gttcctcaac cgcttcggca cgatcagcct gctcaacacg acggtccctg ccaccggttc
     1561 agccgctggt gaccagaacc cctacggcgt cgccgtcgta ccacacagca ccggcgacct
     1621 ggtcaggggc ggggtgctcg tcagcaactt caacaattca cagaaccagc agggaacagg
     1681 gtccagcatc gtcgagatct cgccgagcgg tcaagcgcac gtgttcgccg tcgttccccc
     1741 gctcaccgcg acacccgcgg tcggactcac gaccgccctg gccgtgctgc ccagcgggtt
     1801 cgtggtcgtt ggcagcctgc cggcgccggg tgggaccttg gacggaagtg ccggagggct
     1861 gacgatcctg gatccgaacg ggcacgtcgt cacgacgctc gccgggggcg acatcaacgg
     1921 tccctgggac atgaccgctg tcgaccaggg tcgcaacgcc gtgctgttcg tgactaacgt
     1981 gctcaacggc accgtcgcag cgggcggagc ggtggtcgac ggggggaccg tcgtgcgcat
     2041 cagactcagg attcatcatc agcacatccg cgtgacctca aactcagtga tcgccaccgg
     2101 cttcggtgaa cacaccgacc cgggcgccct ggtcgtcggt ccgaccggag tcggactcgg
     2161 aaagcatgac gtcctctacg tcgccgactc gaacgccaat cggatcgccg cgatccccca
     2221 cgcactgact cgcaaccacg ttctgggagg cggcggcttg acgatctcct cggggggctc
     2281 gctcaacgac ccgctcggcc tcgccatcgc tccaaacgac cacatcatca ctgtcaacgg
     2341 cgccgacggc aacgccgtcg agacgacgcc cagcggggac caggtcgtca ccaagcagct
     2401 cgtgcccaat ggcgcgggag acctgtttgg cctggcagtc acgcccggcg aagacggcct
     2461 ctacttcgtc aacgacatcg gcatgccgga cccggcggcg aattcgctgg agcttctgca
     2521 ctaagccctg aggcgccgtc g
//
LOCUS       DATCCH010000067         8322 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_91208, whole genome shotgun sequence.
ACCESSION   DATCCH010000067 DATCCH010000000
VERSION     DATCCH010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 8322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8322
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91208"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1386
                     /locus_tag="VIJ44_01390"
     CDS             <1..1386
                     /locus_tag="VIJ44_01390"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEY5164581.1"
                     /translation="LFLGCVGKSAQIPLFPWLTDAMEGPTPVSALIHAATMVTAGVFL
                     ICRAHPFFDASHSAMTVVGWIGAATALLAGTMAVLQPDVKRVLAYSTISQLGYMFLAL
                     GTGAYTAAVFFVLMHAFYKACLFLGAGSVLHGNGENQDMRVMGALRRFMPFTAGAMVV
                     AWLAIAGVPPFAGFWAKDGVLEAAYLHHDYGIWAVGVVATIMTGLYMTRLILLTFYGK
                     ERWRPSDSSAVAAVTGGSDDGPDGHDDTEADLGYDPDFLPTVVYGEPPRAPRLHDHEP
                     HESPGLMVLPIVTLALLAIVGGLVNIPLHGFTHLDSWLEPVFRGVTPPVAETFAGAAA
                     LSATALGFGVVGIIVAFVFYRRGLPSPADDPLRARLGVVGDVVGHGYYYDDAISKAVD
                     GPVRAFGTFLDRDVDQRVIDGAVNGIGHLVRRAAEGLRQAQDGLVRRYAIGIALGAAA
                     LLLFFLAYAGR"
     gene            1388..2980
                     /locus_tag="VIJ44_01395"
     CDS             1388..2980
                     /locus_tag="VIJ44_01395"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEY5164582.1"
                     /translation="MTDFPILTAIIATPLIGAVVVALLPGRRPEVIKAVGYGFSVATL
                     GLACWLLADFSTKKVFFQFVESRPWLPAIGSRYIVGVDGISLFMILATALLIPIGLLA
                     SQRYPSQRPKAFTAWFLLLEFSLMGIFLSLDLIIFFLFWEILLVPMYFLIQGWGHERR
                     QYAATKFFIYTAAGSSLMLACTLILGFIHQSDTGVLSFDYRVLAAWSWHGLDGTTEVL
                     LFLGFLAAFAIKVPLVPFHTWLADVHTEAPTAGSVVLAGVILKMGAYGLLRFSFELFP
                     HASVDLAPLLLTLGVIGILYGAIVAAMQKNLKRIIAYSSIAHMGFVIVGIFSLTVIGL
                     DGAVFTMLSHPLTTGALFLLVGMLYERRQTYQLEDYGGIWRSAPILTAMFLVAMFAGI
                     GLPGFSGFIGEFLSLAGTFVSRRPWAVAGAVGVILAAVYMLWAFQRVFMGKPSRINLK
                     LPDVSLREVMVVAPLLALSLFLGLYPKLAIERIEPSLKRSIVNLELKTHFREKRPKGY
                     APTKLPGGVSGTQSIQLGRGAP"
     gene            2977..4467
                     /locus_tag="VIJ44_01400"
     CDS             2977..4467
                     /locus_tag="VIJ44_01400"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEY5164583.1"
                     /translation="MTPPIVAPSINWLAISPEISLVAAGVAIVMLRSVLRRRPIVVPL
                     CTVVAISGVIAAGAVEYRLWHLLRGHRAMTTIAGMVRVDLFGVFLGVVVLAATVMALL
                     LSVAYLRREGLEAPEYFALVLLSAAGMLVMTTANDLIVVFLALEVLSIPLYVLAAFDR
                     RRLSSQEAGMKYFVLGAFSSAVFLYGVALTYGATGTTSLTGIAQFLANNTLLDQGTLL
                     AGIALLLVGLGFKVAAVPFHMWTPDVYQGAPTPVTSFMSAATKAAGFAALLRIFGVAF
                     PLYRSDWRPAVWALAVLSLIVGSVAAVIQTDVKRILAYSSIGHAGFILIGFQANTSRG
                     REAALLYLFVYAFMTIGAFAVITVATRRGDDHHSLDDYRGLALDRPVVGGLLIFFVLA
                     QAGVPFTGGFVAKLEVFSAAAQANEYGLLIIGVLASVVAAFFYLRIALTMLTARDDSA
                     TAPGGLRRRVDIWSAVVLVACVTLVIVVGVVPGTFVHWARDATFLL"
     gene            4535..6157
                     /gene="pgi"
                     /locus_tag="VIJ44_01405"
     CDS             4535..6157
                     /gene="pgi"
                     /locus_tag="VIJ44_01405"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017869387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEY5164584.1"
                     /translation="MTRIVDTPEWAALAHHYDQLGQVHLRQLFADDPDRGDTMNLEVG
                     DLYLDYSKNRLTAETIRLLAALARRAQVEQRRDAMFAGEKINVTERRAVLHVALRAPR
                     DEHIVVDGVDVVPEVHRVLDRMSDFSDRVRSGAWTGHTGRRIRNIVNIGIGGSDLGPR
                     MAYEALKDFSHRSLTVRFVSNVDGTDFWECTRDLDPEETLFVVASKTFTTLETMTNAR
                     TAREWALAALHSDAAVAKHFVAVSTNEAEVTKFGIDPANMFEFWDWVGGRYSYDSAIG
                     LSLMVAIGPDAFRDMLAGFHLIDEHFRTAPIEQNLPMILGLIGIWYDNFFGAETVAIL
                     PYNQYLARLTDYLQQLDMESDGKSVTLEGQPVDWQTGPVIWGQPGTNGQHAYYQLIHQ
                     GTKLIPCDFIGFCRPRHQLGDHHNLLMANLFAQPQALAFGKTADEVRAEGVPEFQVPH
                     RTFAGNHPTNTMLITELTPHTLGQLIALYEHKVFTQGSVWDINSFDQWGVELGKKLAS
                     DIIPELDATEEPTLRHDSSTNTLIRRFRRLRT"
     gene            6154..6723
                     /locus_tag="VIJ44_01410"
     CDS             6154..6723
                     /locus_tag="VIJ44_01410"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY5164585.1"
                     /translation="MTSTPPELWLVRHGATEWSESGRHTGRTDVPLLPSGEERARALA
                     PRLDRHDFALVLTSPLQRARETARLAGYPDAQVDEHLLEWDYGTYEGLTTDEIRAGRP
                     GWTIWNGDPPGGETAAAVEARVRAVIARCTSAAGDALLFAHGHVLRALTAVYLGLGVR
                     AGAQFALETATINVLDHEHEEPTLQRWNA"
     gene            complement(6728..7627)
                     /locus_tag="VIJ44_01415"
     CDS             complement(6728..7627)
                     /locus_tag="VIJ44_01415"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY5164586.1"
                     /translation="MPGPRPRRDGSRARRPGTAFVLSGGGNQGVAQVGMLRALLEHGI
                     VPDVVVGTSAGALNGASIATSPTLEKVDELQRVWVGLRGDQVFPGGALKRAWNILRRD
                     DHLSPNEGLLSVVEAAGVAPDFANLAVPLRVVTTDLDTGDEILFVAGATIPALLASAA
                     IPGIFPPVELHGQRLVDGAVVNLVPISHALAGPTGRIFVLDVSDPLGDRPIRSPLDVA
                     IRAFAISRDQRFELELQWVPPDVEVVVFPAPVDDREFFDFSGGDALIDDAYKMASQTL
                     DDFEARARRRPSRRFLARRRMVP"
     gene            7774..8136
                     /gene="ndhC"
                     /locus_tag="VIJ44_01420"
     CDS             7774..8136
                     /gene="ndhC"
                     /locus_tag="VIJ44_01420"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEY5164587.1"
                     /translation="MTEFYRQYLAVAVLIGAAALMFGAMLGAGRLIRPTRPQQDKYIS
                     YESGSDPVGGWGQSNVRYYIYALLFVIFDVEAVFIFPWAVNLKTLDVFGLVEMTVFIL
                     VLTLGLVYAWRKGVLRWA"
     gene            8127..>8322
                     /locus_tag="VIJ44_01425"
     CDS             8127..>8322
                     /locus_tag="VIJ44_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HEY5164588.1"
                     /translation="MGLVEQGRLPKPLTWLLNYSRKYSLWMFQWGLACCAIEMGAAIA
                     SPRYDMMRLGVIPFPASPRQA"
BASE COUNT         1233 a         3038 c         2574 g         1477 t
ORIGIN      
        1 ctgttcctcg gctgcgtggg caagagcgcg cagattcccc tgttcccgtg gctgaccgac
       61 gcgatggaag gcccgacgcc ggtgtcggcg ctgatccacg ccgccacgat ggtcaccgcc
      121 ggtgtcttct tgatctgtcg ggcccacccg ttcttcgatg cgagtcattc ggcgatgacc
      181 gtcgtcggct ggatcggggc cgccaccgcg ctcctcgcgg gcaccatggc cgtgctgcag
      241 cccgacgtca aacgggtgct cgcctactcg acgatcagcc agctcggata catgttcttg
      301 gcgctcggga ccggcgcgta caccgcggcg gtcttcttcg tgctcatgca cgccttctac
      361 aaggcctgcc tgttcctcgg cgccgggtcc gttcttcacg gcaacggcga gaaccaggac
      421 atgcgtgtca tgggtgcgct ccgacgattc atgccgttca ccgccggcgc gatggtcgtg
      481 gcttggcttg ccatcgccgg cgtgccaccg ttcgccgggt tctgggccaa agacggagtc
      541 ctcgaagccg cgtacctgca tcacgactat gggatctggg ccgtcggggt cgtcgccacc
      601 atcatgactg gcctctacat gactcggctc atcctgttga ccttctacgg caaagaacgt
      661 tggcgcccgt ccgacagctc cgccgtggcc gcggtcaccg gaggatccga cgacggcccc
      721 gacggccacg acgacaccga agccgatctc ggatacgacc ccgacttcct cccgaccgtg
      781 gtgtacggcg agccgccccg agccccccgg ctccacgatc acgaaccgca cgagagccca
      841 gggctcatgg tcctcccgat cgtgaccttg gcgctcctcg cgatcgtcgg ggggctcgtc
      901 aacatcccgc tgcacgggtt cacccacctt gactcgtggc tggagccggt gttccgcggc
      961 gtcaccccgc cggtggccga gaccttcgcc ggcgccgccg cgctcagcgc caccgcgctc
     1021 ggcttcggcg tcgtcggaat catcgtcgcg ttcgtcttct atcgacgggg gttgccctca
     1081 ccggccgacg acccgctccg ggcccgtctc ggggtcgtcg gcgacgtcgt cgggcacggc
     1141 tactactacg acgacgcaat ctcgaaagcc gtcgacgggc cggtccgcgc cttcggcacc
     1201 ttcctcgacc gtgacgtcga ccaacgggtc atcgacgggg ccgtgaacgg aatcgggcac
     1261 ctcgtccggc gcgctgccga aggcctccgt caagcccagg acgggctcgt ccgccggtac
     1321 gcgatcggga tcgccctcgg cgccgccgcg ctgctcctct tcttcctggc ctacgcggga
     1381 cgctgacgtg accgacttcc cgatcctcac cgccatcatc gcgacgccgt tgatcggcgc
     1441 ggtggtcgtc gccctgctcc ccgggcgtcg accggaggtc atcaaggccg tcggctacgg
     1501 gttcagcgtc gccacgctcg gtctggcgtg ctggctgctg gcggacttct ccaccaagaa
     1561 ggtgttcttc cagttcgtcg agagccggcc gtggctgccc gcgatcgggt cgcgctacat
     1621 cgtcggtgtc gacgggatca gcctcttcat gatcctcgcc accgcgttgc tgatccccat
     1681 tggcttgctc gcctcccagc ggtatccgtc gcagcgcccg aaggccttca cggcttggtt
     1741 cttgctgctc gagttctccc tcatggggat cttcctctcc ctggacctca tcatcttctt
     1801 cttgttctgg gagatcctcc tggtgcccat gtacttcctg atccagggct ggggccacga
     1861 acgacgccag tacgcggcca cgaagttctt catctacacc gcggcgggct cttcgttgat
     1921 gctcgcctgc accctcatcc tcggattcat ccaccagtcc gacaccggcg tgctcagctt
     1981 cgactaccgg gttctcgccg cgtggagttg gcacggcctt gacggaacga ccgaggtgct
     2041 gctgttcctc gggttcctcg ccgcgttcgc gatcaaggtc ccgctcgtcc cgttccacac
     2101 ctggctcgcc gacgtccaca ccgaagcgcc gaccgcgggc tcggtggtgc tggccggtgt
     2161 catcctcaag atgggcgcct acgggctgtt gcgcttctcg ttcgagctct tcccgcacgc
     2221 gtcggtggac ctcgcgccgc tgctgctcac cctcggcgtc atcgggatcc tctacggagc
     2281 gatcgtcgcg gcgatgcaga agaacctgaa gcggatcatc gcctactcct cgatcgcgca
     2341 tatgggcttc gtcatcgtcg gcatcttctc gctgaccgtc atcggtctcg acggcgcggt
     2401 gttcacgatg ctcagccacc cgctcacgac cggagcgctg ttcctgctgg tcggcatgct
     2461 ctacgagcgt cgccagacgt accagctcga ggactacggc ggcatctggc gttccgcgcc
     2521 catcctcacc gcgatgttcc ttgtcgcgat gttcgccgga atcgggctgc ccggattctc
     2581 cggattcatc ggcgagttcc tctccctggc cggcaccttc gtctctcgtc gcccgtgggc
     2641 cgtcgcgggc gcggtgggcg tcatcctcgc cgcggtctac atgctctggg ccttccaacg
     2701 ggtgttcatg ggcaagccga gccgaatcaa cctgaaactg cccgacgtca gtctccgtga
     2761 ggtgatggtc gtcgcgccgc tgcttgcgct cagcctcttc ctcggcttgt acccgaagct
     2821 ggcgatcgag cgcattgaac cgtcgctgaa acgcagcatc gtgaacctcg agctcaagac
     2881 ccacttccgc gagaagcggc ccaagggcta cgccccgacg aagctgcccg ggggcgtcag
     2941 cggcacgcag tcgatccagc tcggccgggg cgcaccatga ccccgccgat cgtggccccg
     3001 agcatcaact ggctggccat ctcacctgag atctccctcg tggcggccgg cgtcgcgatc
     3061 gtcatgctgc ggtccgtcct gcggcggcga cccattgtcg tccccctctg caccgtcgtg
     3121 gccatctcgg gagtcatcgc ggcgggcgcc gtcgagtacc ggctctggca tctcctccgg
     3181 ggccaccggg cgatgacgac catcgccggc atggttcgcg tcgatctgtt cggcgtgttc
     3241 ctcggcgtgg tggtgctcgc ggccaccgtc atggcgctcc tcctctcggt cgcgtatctg
     3301 cgtcgtgagg gcctcgaggc gcccgagtac ttcgcgctgg tcctcctgtc cgcagccggg
     3361 atgctggtca tgacgacggc caatgacctc atcgtggtgt tcctcgccct cgaggtgctg
     3421 tcgatcccgc tgtacgtgct cgccgccttc gaccgtcgaa ggctcagctc ccaagaagcc
     3481 gggatgaagt acttcgtgct cggcgcgttc tcatccgcgg tgttccttta cggcgtcgcg
     3541 ctgacctacg gagcgaccgg caccacgtca ctcacgggta tcgcccagtt ccttgccaac
     3601 aacacgctgc tcgatcaggg aacgctcctc gccggcatcg cgctcctcct cgtcgggctc
     3661 ggcttcaagg tcgcggccgt cccgttccac atgtggaccc ccgacgtcta ccaaggtgcg
     3721 ccgacgccgg tgacgtcctt catgtcggcc gcgaccaaag cagctggctt cgccgccctg
     3781 ctgcgcatct tcggagtcgc gtttccgctg tatcgaagcg actggcggcc ggcagtctgg
     3841 gccctggcgg tgctgtccct catcgtcggc agtgtcgccg ccgtcatcca gaccgatgtc
     3901 aaacgaatcc tcgcctactc ctccatcggc cacgccgggt tcatcctcat cgggttccaa
     3961 gccaacacgt cgcgaggtcg cgaggccgcg ctgctctacc tgtttgtcta cgcgttcatg
     4021 accatcgggg cgttcgcggt gatcacggtg gccacccgac gcggcgacga ccatcattcg
     4081 cttgatgact accgaggctt ggcgctggac cgcccggtcg tcggcggtct gctgatcttc
     4141 ttcgtcctcg cccaagccgg ggtgccgttc accggtgggt tcgtcgccaa gctcgaagtc
     4201 ttcagcgccg cggcgcaagc caacgagtac ggcctgttga tcattggcgt gctcgcaagc
     4261 gtggtcgcgg ccttcttcta tctgcgtatc gcgctcacga tgctcaccgc ccgcgacgac
     4321 agcgccaccg cacccggcgg gttgcgtcgc cgcgtcgaca tctggagcgc ggtcgtgctc
     4381 gtcgcttgcg tcaccctcgt catcgtcgtc ggtgtggtgc ccggcacctt cgtccactgg
     4441 gcgcgcgacg cgacgttcct gctctaggaa cgccgcgacc accggtcggt gccgcgatgc
     4501 cccgtaggct gcgcacgacg acaggagacc gccgatgacg cgcatcgtcg acaccccgga
     4561 gtgggccgcg ctcgcgcacc actacgacca gctcgggcag gtccatctcc gccagctctt
     4621 cgccgacgat cccgaccggg gtgacacgat gaacctcgag gtcggggact tgtacctcga
     4681 ctactccaag aaccgcctca ccgccgagac catccgactg ctcgccgccc tcgcccgccg
     4741 agcccaggtc gagcagcgac gcgacgccat gttcgcgggc gagaagatca acgtcaccga
     4801 gcgccgggcc gtgctccacg tcgccttgcg agccccccga gacgagcaca tcgtcgttga
     4861 cggcgtcgac gtcgtgcccg aagtccaccg cgtcctcgac cggatgagcg atttctcgga
     4921 ccgggtgcgc tcgggcgcct ggaccggcca caccggtcgg cggatccgca acatcgtcaa
     4981 catcgggatc ggcggcagcg acctcggccc ccggatggcc tacgaagccc tcaaggactt
     5041 cagccaccga tccctgaccg tccgcttcgt ctccaacgtc gacggcaccg acttctggga
     5101 gtgcacccgg gacctcgatc ccgaggagac cctcttcgtg gttgcctcca agaccttcac
     5161 cacgctcgag accatgacga acgcccgcac ggcgcgggaa tgggccctcg ccgcgctcca
     5221 ctccgacgcc gccgtcgcca agcacttcgt cgccgtctcg acgaacgagg ccgaggtcac
     5281 caagttcggg atcgaccccg ccaacatgtt cgagttctgg gactgggtcg gcggccggta
     5341 ctcctatgac tcggcgatcg ggctctcgct catggtcgcc atcggccccg acgccttccg
     5401 ggacatgctg gcgggcttcc acctcatcga cgagcacttc cgcaccgcgc ccatcgagca
     5461 gaacctcccg atgatcctgg ggctcatcgg catctggtac gacaacttct tcggcgccga
     5521 gaccgtcgcc atcctcccgt acaaccagta cctcgcgcgc ctcaccgact acctccaaca
     5581 gctcgacatg gagagtgacg gcaagtcggt caccctcgag ggtcaaccag tcgactggca
     5641 gaccggaccc gtcatctggg gtcagccggg taccaacggc caacacgcct actaccagct
     5701 catccaccag ggcacgaagc tgatcccgtg tgacttcatc ggcttctgcc gtccccgcca
     5761 ccagctgggc gatcaccaca acctgctgat ggccaacctc ttcgcccagc cccaagcgct
     5821 ggcgttcggg aagaccgccg acgaggtccg agccgaaggt gtccccgagt tccaagttcc
     5881 ccaccggacc ttcgccggga accatccgac caacaccatg ctgatcaccg agctcacccc
     5941 tcacaccctg ggtcagctca tcgcgctcta cgagcacaag gtcttcaccc aaggttccgt
     6001 ctgggacatc aactcgttcg atcaatgggg tgtcgagctc ggcaagaagc tggcgtccga
     6061 catcatcccg gagctcgacg ccaccgagga accgacgctt cgacatgact cctcgacgaa
     6121 cacgctcatc cgccggttcc gtcgactccg gacgtgacgt cgaccccgcc cgagctgtgg
     6181 ctcgtacgcc atggcgcgac cgagtggagc gagagcggac gccacaccgg ccggaccgac
     6241 gtcccgctcc tgccgagcgg tgaagagcgg gcccgcgcgc tcgccccccg gctcgaccgc
     6301 cacgacttcg cgctcgtgtt gacgagcccg ttgcaacgcg ctcgggagac ggcccggctc
     6361 gcgggctatc cagacgcgca ggtggacgag cacctgctcg aatgggacta cggcacgtac
     6421 gagggactca ccaccgatga gatccgcgcc ggtcgcccgg gatggacgat ctggaacgga
     6481 gaccctccgg gcggtgagac cgcggccgcc gtggaagcgc gcgtccgggc ggtgatcgcg
     6541 cggtgcacct cggccgccgg cgacgcgctg ctcttcgcgc acggtcacgt cctgcgggcc
     6601 ctcaccgccg tctacctagg cctcggtgtc cgggccgggg cccagttcgc cctcgagacc
     6661 gccaccatca acgtgctcga ccatgagcat gaggagccga ccctgcaacg ctggaacgcc
     6721 tgagcgctca gggcaccatc cggcgccggg ccaagaaccg tcttgacggc cgtcggcggg
     6781 cgcgggcctc gaagtcatcg agcgtctggg acgccatctt gtaggcatcg tcgatcagcg
     6841 cgtcaccgcc ggagaagtcg aagaactccc ggtcgtcgac gggcgcaggg aagacgacga
     6901 cctcgacgtc gggaggcacc cactggagct cgagctcgaa tcgctgatcg cgcgagatcg
     6961 cgaacgcccg gatcgccacg tcgagcggcg atcgaatcgg ccggtccccg agcgggtctg
     7021 agacatcgag gacgaagatc cgccctgtcg gacccgcgag cgcatgggag atcggcacca
     7081 ggttcacgac cgcaccgtcc acgagccgtt ggccgtgcaa ctcgacgggc gggaagatgc
     7141 cggggatcgc cgcgctcgcc agcaaggccg ggatggtcgc gcccgccacg aacaggatct
     7201 cgtcaccggt gtcgaggtcc gtggtgacga cccgcagcgg gacggcaagg ttcgcgaaat
     7261 cgggagcgac tcccgcggcc tcgacgaccg agagcagccc ctcgttcggg gacagatgat
     7321 cgtcgcggcg caagatgttc catgctcgct tgagggcccc gcccgggaag acctggtccc
     7381 cgcgtaaccc cacccagacg cgctgcagct cgtcgacctt ctccagggtc ggcgacgtgg
     7441 cgatcgaggc gccgttcagc gcaccggcgg aggttccgac gaccacgtcg gggacgatcc
     7501 cgtgctccaa cagcgcgcgg agcatcccga cctgggccac accctggttg ccgccgcccg
     7561 agagcacgaa cgccgtgccc ggccgccgag cccggctgcc gtcgcgccgc ggtcgcggtc
     7621 ctggcatgca cggcctcctt ctcgtctcgg tcatcgtcgc gcagggaccg gcgcgccgac
     7681 ggtttcaggc tccgacagtt caggcgtgga ggaccgccgc tgatagcctg cgcgcccggt
     7741 gcatccccgg gggatcgaga gggccggctc gctgtgaccg agttctaccg gcaatacctg
     7801 gccgtcgcgg tcctgatcgg cgctgccgcg ctcatgttcg gggcgatgct cggagccggg
     7861 cgcctcattc ggccgacgcg gccgcagcaa gacaagtaca tcagctacga gtccggttcc
     7921 gaccccgtcg gcggatgggg ccagtcgaac gtccggtact acatctacgc gctcctcttc
     7981 gtcatcttcg acgtcgaggc ggtgttcatc ttcccgtggg ctgtcaacct caagaccctc
     8041 gacgtgttcg gcctggtgga gatgaccgtc ttcatcctgg tgctcacgct cggtctcgtc
     8101 tacgcgtggc gcaagggggt cttgcggtgg gcctagtcga gcagggtcgg ctcccgaaac
     8161 cgctcacgtg gctgctcaac tacagccgca agtactcgct ctggatgttc cagtggggcc
     8221 tcgcctgctg cgccatcgag atgggcgcgg cgatcgccag tccccgctac gacatgatgc
     8281 gcctcggggt catcccgttc ccggccagcc ctcgccaagc cg
//
LOCUS       DATCCH010000068         2662 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_173891, whole genome shotgun sequence.
ACCESSION   DATCCH010000068 DATCCH010000000
VERSION     DATCCH010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173891"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1754
                     /locus_tag="VIJ44_01430"
     CDS             <1..1754
                     /locus_tag="VIJ44_01430"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEY5164589.1"
                     /translation="LDGRGDPEVPLIVPPGAFGEPISIDASELETVLAAMAEASDPAA
                     GSNSWALAGHRTHSGSPLVAGDPHRLVEVPGVYYQCHLACPDFDAVGLAFVGVPGFPH
                     FGHTARVAWCVTNAYGDYQDLYVERLDGIPPSRTEVIAVRDAEPVTIDCYETTHGPVL
                     FGDPGAGIGIALRATALAEPSAGLAVVLPMLRADTVDELDEAMRAWVDPVNNLVSADV
                     DGHLRYRTVGVIPERSAANAWGPVPGWTAAHEWTGVVPYDELPWLRDPDRGCIVAANQ
                     QIVGPDYPHYLGFEYSRPDRARRVFARLAELDAATVDDMAAIHRDRRSLGADAWVERL
                     TDLKGRDHTERRALEQLRGWDCVMDAASTAAAIYVATRDHAGRALAHQPRLAPLRRPV
                     AGEPIGTFQPLELRLWTLLPGLLAADDTTLLGPGATWDTVLADALSAAVADLRTRLGD
                     DVDTWRWGALHRCAPNHPLAGDDPALAARLNPPPVEVGGEWDTVFAAAHPAGYGFGVT
                     TASVARYVFDLADWDQSRWIVPLGASGEPTNPHFADQQKAWASGELVPMRYRWDVIAD
                     TAESTTTLRPSLPSRAS"
     gene            complement(1740..>2662)
                     /locus_tag="VIJ44_01435"
     CDS             complement(1740..>2662)
                     /locus_tag="VIJ44_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164590.1"
                     /translation="ARVLVPSPPAVTDPPWSADDPVDPGGAADGRTVVSPVPNADLTW
                     DDLARGDAALAAWCADRWLGCWRPLAPVTDLAALAVTRRSWHTVAEHVLAPFRFQACS
                     KIGLRYTPGGVGIPFVRGDGVEVQLRLDESGLVVDRGSETRAPWTTLRAAADAAGVAV
                     GVRTGVYEPTTAGEPDQPLAVDADAAARLADWFGFGASVLEEVRAAARDGSSNRLQLW
                     PEHFDVSVDLGDEAAGQRGTFGASPGDDGHSLPYLYVTHWGPVPDDRYWNDTVFSGAS
                     LGYDRLVGLKDPRRVAIEFLAGGRARLAGA"
BASE COUNT          384 a         1122 c          821 g          335 t
ORIGIN      
        1 gactcgacgg acgcggcgac cctgaggtcc cgctgatcgt cccgccggga gcgttcggcg
       61 aaccgatctc catcgacgcc tccgagctcg aaaccgtgct cgccgccatg gccgaagcca
      121 gcgaccccgc ggccggctcg aactcgtggg ccctcgccgg ccaccgcacc cactccgggt
      181 caccgctcgt cgccggggac ccgcaccgtc tcgtcgaggt tcccggcgtc tactaccaat
      241 gccatctcgc ctgccccgac ttcgacgccg tcggcttggc cttcgtcggt gtccccggct
      301 tcccccactt cggtcacacc gcccgcgtcg cctggtgcgt caccaacgcg tacggcgact
      361 accaggatct ctacgtcgag cggctcgacg gcatcccacc ctcccgcact gaggtgatcg
      421 cggtccgcga cgccgaaccg gtcaccatcg actgctacga gacgacccac ggcccggtcc
      481 tgttcggaga ccccggcgcc gggatcggaa tcgcgcttcg cgccaccgcc ctcgccgagc
      541 cgagcgccgg tctcgcggtc gtgctcccca tgctccgggc cgacaccgtc gacgagctcg
      601 acgaggcgat gcgcgcctgg gtcgacccgg tcaacaactt ggtctcggcc gacgtcgacg
      661 gccacctccg ctaccgaacc gtcggcgtca tccccgaacg ctccgccgcc aacgcctggg
      721 gtccggtacc gggctggacc gcggcccacg agtggaccgg cgtcgttccc tacgacgagc
      781 tgccctggct gcgcgacccc gaccgaggct gcatcgtcgc cgccaaccag cagatcgtcg
      841 gcccggacta cccgcactat ctcggattcg agtacagccg tcctgaccgg gcccgccggg
      901 tcttcgcccg actcgccgag ctcgacgccg ccaccgtcga cgacatggcc gccattcacc
      961 gggaccgacg gtcgctgggc gccgacgcgt gggtggagcg cctcacggac ctcaagggac
     1021 gcgaccacac cgagcgacgc gccctcgagc agctgcgcgg gtgggactgt gtcatggacg
     1081 ccgcctccac cgcggcggcg atctacgtcg ccacccgcga ccacgccggt cgggccctgg
     1141 cccaccagcc tcgcctcgcg ccgctgcggc gacccgtcgc cggcgagccc atcgggacgt
     1201 tccagcccct cgagctgcgc ctctggaccc tgctcccggg gttgctcgcc gccgacgaca
     1261 ccaccctcct cggtccgggc gcgacgtggg acaccgtcct cgccgacgcg ctcagcgccg
     1321 cggtcgccga cctgcggacc cgcctcggcg acgatgtcga cacctggcgc tggggggcgc
     1381 tgcaccgatg cgcgcccaac catcccctcg ccggggacga cccggccctg gccgcccgac
     1441 tcaacccccc gccggtcgag gtcggcggcg agtgggacac cgtgttcgcc gccgcgcacc
     1501 cagccggcta cggattcggt gtcacgaccg cgtcggtcgc ccgctacgtc ttcgacctcg
     1561 ccgactggga ccagtcgcgc tggatcgtcc cgctcggcgc gtccggtgag cccactaacc
     1621 cccacttcgc cgaccagcag aaggcctggg cctccggcga gctcgtcccg atgcgctacc
     1681 gctgggacgt catcgccgac accgccgagt cgacgacgac gttgcgcccc tcgttgccgt
     1741 cacgcgccag ctaagcgcgc ccggcccccg gccaggaact caatcgccac ccgccgcgga
     1801 tccttgagac cgaccagccg gtcatagccg agactggcgc ccgaaaacac ggtgtcgttc
     1861 cagtaccgat cgtcgggcac cgggccccaa tgcgtcacat agagataggg gagcgagtgc
     1921 ccgtcgtcgc ccggcgacgc cccgaacgtt ccccgctgcc cggccgcctc gtccccgaga
     1981 tcgaccgaca catcaaaatg ctccggccac agctgcagcc ggttcgacga accgtcccgc
     2041 gccgcggcgc gcacctcctc caacaccgac gccccgaacc cgaaccagtc ggccaggcgc
     2101 gccgcggcgt ccgcgtccac cgccaacggc tggtccggct ctcccgcggt cgtcggctca
     2161 tacacccccg tgcgcacacc gacggcgaca cccgccgcgt ccgcggcggc ccgcaacgtc
     2221 gtccacggcg cccgcgtctc cgaaccccga tccacgacca ggccggactc gtcgagccgg
     2281 agctgcacct cgacgccatc cccccgcacg aacgggatcc ccacacctcc cggcgtgtac
     2341 cgcaacccga tcttcgagca cgcctgaaac cgaaacgggg cgaggacatg ctcggccacg
     2401 gtgtgccacg agcggcgcgt gaccgccaac gcggccaggt cggtcaccgg cgccaacggc
     2461 cgccagcatc ccagccaccg gtcggcacac cacgccgcca acgccgcgtc gccccgggca
     2521 aggtcgtccc aggtcaggtc ggcgttcgga accggtgaca ccaccgtgcg cccgtccgcc
     2581 gcgccgcccg ggtcgaccgg gtcatcggca gaccacggcg ggtcggtcac cgccggcggt
     2641 gacggcacca gcacccgagc ct
//
LOCUS       DATCCH010000069         3775 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_173958, whole genome shotgun sequence.
ACCESSION   DATCCH010000069 DATCCH010000000
VERSION     DATCCH010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3775
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173958"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /locus_tag="VIJ44_01440"
     CDS             complement(<1..910)
                     /locus_tag="VIJ44_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005517232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5164591.1"
                     /translation="MTTALDPLDAHPELFLPEPEPRPVRYTIISVDDHLVEPPHMFEG
                     RLPAGLQDRAPRVVEDDHGHEVWEFEGERHFQVGQNAVAGRRLETVKIEPFRFDEMRR
                     GCWDIEARIHDMDINGVWASLNFPSMITGFCGRVYSQAKDPELGLAVTRAWNDWFFDE
                     WYSAHPDRIVPMGITWLADPEIGAAEICRNAERGFRSVTLPERPHRIGFPSIFDEAWE
                     PIIAACAETGTVISLHVGSSGMPEFPPGCPPLQLGATLFGQLSLTACAEWVWSAWAVK
                     YPKLKIAMSEGGIGWVGMLIDRLDNIV"
     gene            complement(913..2175)
                     /locus_tag="VIJ44_01445"
     CDS             complement(913..2175)
                     /locus_tag="VIJ44_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014000411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY5164592.1"
                     /translation="MTVGSVPTSAVLDGFDLTDLDNFADGFPHDAFVTHRHRAPVLWH
                     EPTPHTPDGDGFWSVATHAETLAVTRDPLTYSSERGGHRERAGTILPDQAIAGEVLNM
                     MDDPRHGRIRRLVSIGLTPKAVTDLEPDLRARMRVILDDVDAETCDVVALVRELPLHA
                     IALLMGVPGTDRHQLGEWVDHAFDFKDRDYLETTDEVAAAQRDMFDYGTRLIAEKRRH
                     PSDDMLSVVANARLDDEDPPQLTDGELQLFFSLLFSAGAETTRSAAAGGLLALAERPE
                     QWRRLRQDRSLVGHAVEEIVRWTSPAAYNRRTATCVTELGGMAVQPGDKVVFWEASAN
                     RDERIFERSMSFDVSRDPNPHLGFGHGLHFCLGASLARLELRITLEELTARLERLEPA
                     GAPEWTRSNKHTGLRHVPLRLDWAEMRS"
     gene            2269..2730
                     /locus_tag="VIJ44_01450"
     CDS             2269..2730
                     /locus_tag="VIJ44_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164593.1"
                     /translation="MATLRFNHMELTFPPGTLDKAFRREVTAFYGELFGWDSSDVEVV
                     GQRCQYLRVDDGQFVLLAESERPMQSPGYDHLGLLCETRAEVDELAARAKTYQERDDR
                     VRVKEYEDLVTANLTVHAFYVKYLLPIWCDVQCMEWQAGAAPGRRWQYTSA"
     gene            2745..>3775
                     /locus_tag="VIJ44_01455"
     CDS             2745..>3775
                     /locus_tag="VIJ44_01455"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY5164594.1"
                     /translation="MDFNPFSPDFFDDPYETYRWLRDHAPAYRNETFGFWALSRYEDV
                     VSAHRDWKTFSSEHGVTIDQLTDPVSPVRGTSIIMLDPPEHEHLRKLVSRVFTPRAVE
                     ALEPMVRGVVRSHLDPLMDRDEFDLVADFSGPFPVEVISTMLGVPDADRQQIRHWTDL
                     LLHREPNDPRPTHEGMEAALLQVGDFFDLIADKRATPGDDLITRLIEVEVTDEDGTTH
                     QLTDGDIAGFAVLLAAAGSETVTKLVGSGVVLFDRNPDEWRQLVNDRSQAANAVEEVL
                     RYWAPSQYQGRFSLAASTWHGVTIPEGMPVLLITGAASRDERAYDDPDRFDIDRPQPL
                     GVGLGHGIHA"
BASE COUNT          594 a         1250 c         1337 g          594 t
ORIGIN      
        1 cgacgatgtt gtcgagccgg tcgatgagca tcccgaccca gccgataccg ccctccgaca
       61 tcgcgatctt gagcttcggg tacttcaccg cccacgccga ccacacccat tcggcgcacg
      121 cggtgagcga cagctgaccg aacaaggtgg cgccgagctg caggggcggg cagcccggtg
      181 ggaactccgg catgcccgac gaaccgacat gcaagctgat gacggtgccg gtctcggcgc
      241 acgcggcgat gattggctcc caggcctcgt cgaagatcga cgggaacccg atgcggtgcg
      301 gccgttcggg cagcgtgacc gaccggaagc cccgctcggc gttgcggcaa atctcggcgg
      361 cgccgatttc ggggtcggcg agccacgtga tccccatcgg gacgatgcgg tccgggtgcg
      421 cggagtacca ctcgtcgaag aaccagtcgt tccaggcccg ggtcaccgcc aggcccaact
      481 cggggtcctt ggcctgggag tacacccgtc cgcagaagcc ggtgatcatc gacgggaagt
      541 tcagtgacgc ccacacgccg ttgatgtcca tgtcgtggat gcgggcctcg atgtcccagc
      601 agcctcggcg catctcgtcg aaccggaacg gctcgatctt gactgtctcg agtcgacgtc
      661 cggcgaccgc gttctggccg acctggaagt ggcgttcacc ttcgaactcc cagacctcgt
      721 ggccgtggtc gtcctcgacg acgcggggcg cgcggtcttg gaggccggcg gggagccgcc
      781 cttcgaacat gtggggtggc tcgacgagat ggtcgtcgac cgagatgatc gtgtatcgaa
      841 cggggcgggg ctcgggttcg gggaggaaca gctcggggtg cgcgtcgagc ggatccagcg
      901 cggtcgtcac ggtcagctcc tcatctcggc ccagtccaac cgcaagggta cgtggcgcag
      961 tccggtgtgc ttgttgctgc gggtccactc gggggcgccg gccggctcga ggcgctccaa
     1021 ccgggccgtg agctcctcga gggtgatgcg cagctcaaga cgggccaggc tcgccccgag
     1081 acagaaatgc aggccgtgcc cgaaccccag gtgcgggttc gggtcgcggc tcacgtcgaa
     1141 cgacatcgac cgctcgaaga tccgctcgtc tcggttggcc gacgcctccc agaacacgac
     1201 cttgtcgccc ggttggacgg ccatcccgcc gagctcggtg acgcacgttg cggtgcgacg
     1261 gttgtacgcg gccgggctgg tccagcggac gatctcctcg acggcgtggc cgacgagcga
     1321 tcggtcctgg cggaggcggc gccactgctc gggccgctcc gcgagggcga gcagcccgcc
     1381 cgccgccgcg ctgcgtgtcg tctcggcgcc agcggagaac agcagagaga agaacagctg
     1441 cagctcaccg tcggtgagct ggggtgggtc ctcgtcgtcg agtcgggcgt tggcgacgac
     1501 ggacaacatg tcgtcgctcg ggtggcgtcg cttctcggcg atgagccggg tgccgtaatc
     1561 gaacatgtcg cgttgcgcgg cggcgacctc gtcggtggtc tcgaggtagt cgcgatcttt
     1621 gaagtcgaag gcatggtcga cccactcgcc gagctgatgc cggtcggtgc cggggacacc
     1681 catcagcaac gcgatggcgt gcagcggcag ctcgcgcacc aaggcgacga cgtcgcaggt
     1741 ctcggcgtcg acgtcgtcga ggatgacccg catccgggct cgcaggtcgg gttccaggtc
     1801 ggtgacagcc ttgggggtga gcccgatgct caccagtcgt cggatccggc cgtgtcgagg
     1861 gtcatccatc atgttgagga cctcgccggc gatcgcctgg tcgggcagga tcgtcccggc
     1921 gcgttcccgg tggccgcccc gctcggagga gtaggtgagc gggtcgcggg tcacggcgag
     1981 ggtctcggcg tgggtggcga ccgaccagaa gccgtcgccg tcgggagtgt ggggggtcgg
     2041 ttcgtgccac agcaccggag cccggtggcg gtgggtgacg aacgcgtcgt ggggaaagcc
     2101 gtcggcgaag ttgtcgaggt cggtcaggtc gaagccgtcg agcaccgccg aggtgggtac
     2161 cgacccgacc gtcacggacg gcaatctacg cggcgagcca cgaccccgga cccgaggtgc
     2221 gggggtaggg gcgtgcgggt cgcgtcctgc acggcgtacg gtgcaggcgt ggcgacgctg
     2281 cgcttcaacc atatggagct gacgttcccg ccggggacgc tcgacaaggc gttccgccgc
     2341 gaggtcacgg cgttctacgg cgagctgttc ggctgggatt caagcgacgt cgaggtcgtc
     2401 gggcaacgct gccagtatct ccgggtcgac gacggccagt tcgtgctgct cgccgagtcc
     2461 gaacggccga tgcagtcgcc cggttacgac cacctcgggc tgctctgcga gacccgcgcc
     2521 gaggtcgacg agctggcggc gcgggcgaag acgtaccagg aacgcgacga tcgggtccgg
     2581 gtgaaggaat acgaggatct cgtcaccgcc aacctgaccg tccacgcgtt ctacgtgaag
     2641 tacctcctgc cgatctggtg cgacgtgcag tgcatggagt ggcaggcggg ggctgcgccc
     2701 ggtcgtcgct ggcagtacac gtcggcgtag cccatccaga cgcgatggac ttcaacccgt
     2761 tctcacccga cttcttcgac gacccgtacg agacgtaccg gtggctgcgt gatcacgccc
     2821 cggcgtaccg caacgagacg ttcgggttct gggccttgtc ccggtacgag gatgtggtgt
     2881 ccgcccaccg cgactggaag acgttcagca gcgagcacgg cgtcaccatc gaccagctca
     2941 ccgaccctgt cagcccggtg cggggcacga gcatcatcat gttggacccc ccggagcacg
     3001 agcatctccg caagctggtc agccgggtgt tcacgccccg cgcggtcgaa gcgttggagc
     3061 cgatggtccg cggcgtcgtg cgctcgcatc tcgatccgct catggaccga gacgagttcg
     3121 atctcgtggc ggacttctcc ggtccgttcc cggtcgaggt catctcgaca atgctcggcg
     3181 tccccgacgc cgaccggcaa caaatccgcc actggaccga cctgttgctg caccgtgagc
     3241 ccaacgaccc ccggccgacc cacgaaggca tggaagccgc gctgctccag gtcggcgact
     3301 tcttcgatct catcgccgac aagcgggcca cgccgggcga cgacctgatc acccggctca
     3361 tcgaggtgga ggtcaccgac gaggacggca ccacccacca gctcaccgac ggtgacatcg
     3421 cggggttcgc ggttctgctc gccgccgcgg ggagcgagac ggtgaccaag ctcgtcggca
     3481 gcggcgtcgt gctgttcgat cggaaccccg acgagtggcg tcagctggtc aacgaccggt
     3541 cccaggccgc gaacgcggtc gaagaagtcc tgcgctactg ggcaccgtcg caataccagg
     3601 ggcggttctc actggccgcg tcgacctggc atggcgtgac gatcccggag gggatgccgg
     3661 tcctgctcat caccggcgcc gcgagccgcg acgaacgcgc ctatgacgac cccgaccggt
     3721 tcgacatcga ccgcccccag ccgctcgggg tcggactcgg acacgggatt cacgc
//
LOCUS       DATCCH010000070         2204 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_465067, whole genome shotgun sequence.
ACCESSION   DATCCH010000070 DATCCH010000000
VERSION     DATCCH010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465067"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..826
                     /locus_tag="VIJ44_01460"
     CDS             182..826
                     /locus_tag="VIJ44_01460"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY5164595.1"
                     /translation="MPLEAGHERRARSASANVWCAIGIRDMNAAEIDALLEVVTRSER
                     PRTTPEERITAVEDRRAIRDLIMVYGYLCDARRWDDLLALYTDDIERVLAGSLTERVQ
                     GKATLREKLVAPTLERTDGDGAAPPPEYLVTLGLRHLMASDVIRLSDEGTTATAAVQY
                     TMVATADDERGFRRGSHEGSYVFDFRKEAGVWRFSRQLVTSDNAHNPMFQREST"
     gene            843..1616
                     /locus_tag="VIJ44_01465"
     CDS             843..1616
                     /locus_tag="VIJ44_01465"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5164596.1"
                     /translation="MSDATSGRFAGRVAMVTGAGAGIGRATVIRLAADGAAVVVNDVR
                     EDLAADTVAAVTAAGGRAVLAPGDVTVAADVDAIVARAHDAAGPVDVLINNVGGAPAG
                     VAWRELRETSIEDFYAFVALNLGSAFICSRAVVGGMVERGYGKIVCVNSISAVFGQRA
                     GVGYASAKAGLSGFVASLAKEVAKAGVNVNAVLIGNAPHPSRTPEREAVLNQWNHFGR
                     HGRHEEFAGTIAFLVSDDATYLSGSTVVVDGGTLRFALL"
     gene            1622..>2204
                     /locus_tag="VIJ44_01470"
     CDS             1622..>2204
                     /locus_tag="VIJ44_01470"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY5164597.1"
                     /translation="MRDLVTTPGRREDFVRRGVWDDATLAGRVAHLAASRADAVAVVD
                     EGGQRRRTYAELAGEAGCLSARLRERGVTVGDVVSVQLPNWYEAAVVAVAIQSIGAVI
                     NPLLPNYRARELTHVFTTAQPAAIVTPGEYRGFDHRRLVADVSAATGVRPLHIVSRAE
                     TGESGDVTLDDLLDGSAVSLDTSTVNAEAVSELI"
BASE COUNT          335 a          742 c          789 g          338 t
ORIGIN      
        1 gagtctcacc cggctcgtcg gcgcccgcca cacggatccg cagcggaggg cggctcggga
       61 aaccgccggc cgccctccgc gctcatgacc gggcgacgcg acaccgagcg ccggtgggtc
      121 aaaatggggt tgtgggcatc accgaacggg tcgacgggta ctcgaagcac tgcagcacgt
      181 cgtgcctctc gaggcgggtc atgaacgacg agcgcgatcg gcgagcgcga acgtgtggtg
      241 tgccatcggg atcagagaca tgaacgccgc cgagatcgac gccctgctgg aggtggtgac
      301 ccgaagcgag cgtccccgga ccacccccga ggagcggatc accgcggtgg aggaccgacg
      361 ggcgatccgt gacctcatca tggtctacgg ctacctctgc gacgcccgcc gctgggacga
      421 cctcctcgcg ctctacaccg acgacatcga acgggtgctc gccggcagcc tcaccgagcg
      481 cgtccagggc aaggcgacgc tgcgcgagaa gctggtggcc cccacactgg agcgcaccga
      541 cggtgacggc gcggccccgc cacccgagta cctcgtcacc ctcggactcc gccacctcat
      601 ggcgagcgat gtgatccgac tctccgacga agggaccacc gcaacggctg cggttcagta
      661 cacgatggtc gccaccgccg acgacgagcg cgggttccgt cgtgggtctc acgaaggctc
      721 gtacgtgttc gatttccgca aggaagccgg cgtctggcgc ttctcccgcc agctcgtcac
      781 cagcgacaac gcgcacaatc ccatgttcca gcgcgagtcg acgtgagccg cgtgcggccc
      841 cggtgagcga cgcgacgtcg ggtcgtttcg cgggccgggt ggcgatggtc acgggagccg
      901 gagccgggat cggtcgggcc accgtcatcc gcctcgcggc tgacggcgcc gcggtggtcg
      961 tgaacgacgt tcgcgaggac ctggcggctg acaccgtcgc tgcggtcacg gccgccggtg
     1021 gtcgcgcggt gctcgcccca ggggacgtga ccgtcgccgc ggacgtggac gcgatcgtgg
     1081 cccgggccca cgatgccgcg gggccggttg acgtgctgat caacaacgta ggcggcgcgc
     1141 ccgcgggcgt cgcctggcga gagctgcgcg aaacgtcgat cgaggacttc tacgccttcg
     1201 tggccctcaa tctgggatcg gccttcatct gctcacgggc ggtcgtcggt ggaatggtcg
     1261 agcgcggcta cggcaagatc gtctgcgtca actccatctc agcggtcttc ggtcagcggg
     1321 ccggcgtcgg atacgcgtcg gcgaaggcag gtctgagcgg gttcgtggcg tccctcgcga
     1381 aggaggtggc gaaggcgggc gtgaacgtga acgccgtgtt gatcggcaat gcgccccacc
     1441 cgtcgcgaac ccccgagcgg gaggccgtcc tcaaccagtg gaaccatttt gggcgccacg
     1501 ggcgccacga agagttcgcg ggcacgatcg cgttcttggt gtccgacgat gccacgtacc
     1561 tctcgggctc cacggtcgtc gtcgacggcg ggaccttgcg attcgcgctg ctatgagtcc
     1621 gatgcgcgac cttgtgacca ccccgggccg ccgcgaggac ttcgtgcgcc ggggggtctg
     1681 ggacgacgcg acgcttgccg gtcgggtcgc ccacctggcc gcgtcacgag cagatgccgt
     1741 cgcggtggtc gacgaaggcg gccagcggcg gcggacctac gccgagctcg cgggtgaggc
     1801 cgggtgtctc tcggcgcggt tgcgagaacg cggcgtgacg gtcggagacg tggtgtcggt
     1861 ccaacttccg aactggtacg aggccgcggt cgtcgcggtg gcgatccagt ccatcggggc
     1921 ggtgatcaac ccgctgctcc caaactaccg ggctcgtgag ctgacgcacg tgttcaccac
     1981 ggcgcagccg gcggcgatcg tcaccccggg tgagtatcga gggttcgatc atcgccgcct
     2041 cgtcgccgat gtgtcggcgg cgactggcgt gcgaccgttg cacattgtct cgcgagcgga
     2101 gaccggcgag agcggcgacg tgaccctcga cgacctgctc gacggttcgg ccgtctccct
     2161 cgacacttca accgtgaacg ctgaagctgt ctcggagctg atct
//
LOCUS       DATCCH010000071         2132 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_45657, whole genome shotgun sequence.
ACCESSION   DATCCH010000071 DATCCH010000000
VERSION     DATCCH010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45657"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..1083
                     /locus_tag="VIJ44_01475"
     CDS             163..1083
                     /locus_tag="VIJ44_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY5164598.1"
                     /translation="MPEVRELATGLEFPEGPVAQPDGSTLLVEIGRGTVSRVTTEGAV
                     EVVADLGGGPNGAAIGPDGACYVVNNGGFLWSNVGDLRIPLDLATGTNEPPGFTGGWV
                     ERVDLSTGTSRVLYRDCDGHPFRSPNDIVFDDAGGFWFTDLGKTRARDMDKGGLYYAS
                     PDGSSVRCVGFGTLGANGVGLSPGGDRVYVAESFTGRLWGWDLEGPGQVKATGTGPGG
                     GHCLAATKGHFDSLAVEEDGTVVVAAIGQGLCVVRPDFSHEYVAMPDPFTTNICFTGA
                     DRRTALVTLSGGGRLVAVDWPRPGLELNYS"
     gene            1080..>2132
                     /locus_tag="VIJ44_01480"
     CDS             1080..>2132
                     /locus_tag="VIJ44_01480"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY5164599.1"
                     /translation="MTSIAEFVRAREDDGRRALRFEDQTWTWSEFVRAAAQRAAYLDA
                     ELDPDCPPHVGVLLDNVPEFPMWLSAAALAGDAIVGINPTRRGRELERDIRHADCQLI
                     VTEARYLDLLENLDLGPATGRVVIVEDAGDVLAAHRDAPVPDRRVDERGIYLLLFTSG
                     TSGAPKACILSQGRLAQQASRLSQMMAFSADDVMYQVMPLFHSNAIITGFAPWLISGA
                     TCVLRRKFSASGFLPDVRHYGVTYFNYVGKPLSYILATPERPDDAQNTLTRVFGNEAA
                     DLDIDRFSRRFAVPVVDGYGSTEGGANVNRTPETPKGALGVAPDGTVILDSETGEECP
                     RARFDGDGHLLNPEEAI"
BASE COUNT          321 a          750 c          735 g          326 t
ORIGIN      
        1 gtcgacatcg atcacccact cgggatcggg caccgcgcgc tcccctcctg gcctgttggc
       61 gtgccgtccg gttccggagg tccgccgggc gaccgtacct ggtcatcgcc acctcggcta
      121 gaaacactcc aggcgaaccc gtggggggaa acgatggctg acgtgcccga ggtccgcgag
      181 ctggcaacag ggctggagtt cccggaaggc cccgtcgcgc agcccgatgg cagcacactc
      241 ctggtcgaaa tcggccgggg aaccgtgagc cgggtcacca ccgagggggc ggtcgaggtc
      301 gtcgccgacc tcggcggcgg tcccaacggc gcggcgatcg gacccgacgg agcgtgctat
      361 gtcgtcaaca acggcgggtt cttgtggagc aacgtcgggg acctgaggat cccgttggat
      421 ctcgccaccg ggaccaacga gccgcccggc ttcaccggcg ggtgggtcga gcgggtggac
      481 ctctcgaccg ggacgtcccg cgttctgtac cgagactgtg acggccaccc gttccgcagc
      541 cccaacgaca tcgtgttcga cgacgccggc ggcttctggt tcaccgatct cggcaagacc
      601 cgggcgcgcg acatggacaa ggggggtctg tattacgcca gccccgacgg gtcgtcggtc
      661 cgctgcgtcg ggttcggaac gctgggcgca aacggggtgg gcctctcccc cggcggcgac
      721 cgggtgtacg tcgccgagag cttcaccggt cggctatggg ggtgggacct cgaggggccg
      781 ggccaggtca aagcgaccgg gaccggaccc ggcggcggcc actgcctcgc cgcgacgaaa
      841 ggccacttcg actcgctcgc ggtcgaggag gacggcacgg tggtcgtcgc cgcgatcggc
      901 cagggcctct gcgtggtgcg acccgacttc agccacgagt acgtcgcgat gcccgacccg
      961 ttcaccacca acatctgctt caccggcgcc gatcggcgca ccgccctggt caccctctct
     1021 gggggcggcc gcttggtcgc ggtcgactgg ccccggccgg gcttggagct caactactcg
     1081 tgacgtcgat cgcggagttc gtccgggctc gagaggacga cgggcggagg gcgctgcgtt
     1141 tcgaggacca gacctggacc tggtcggagt tcgtccgcgc cgcggcgcaa cgcgccgcgt
     1201 acctcgatgc ggagctcgac ccggactgcc cgccacatgt cggggtcctc ctcgacaacg
     1261 tcccggagtt cccgatgtgg ctcagcgccg cggcgctggc cggagatgcg atcgtgggaa
     1321 tcaacccgac ccggcgcggg cgggagctcg agcgcgacat ccgccacgcc gactgccagc
     1381 tcatcgtcac cgaggcccgc tacctcgatc tcctcgagaa tctcgacctc gggccggcga
     1441 ccgggcgggt cgtgatcgtc gaggacgccg gcgacgtcct cgcggcccat cgtgacgcac
     1501 cggttccgga tcgccgagtc gacgagcggg gcatctacct gctgctgttc acgtccggga
     1561 cgagcggcgc gccgaaggcc tgcatcctct cccagggacg gctggctcag caggccagcc
     1621 gcctgagcca gatgatggcc ttctcggccg acgacgtcat gtaccaggtg atgccgctct
     1681 tccattccaa cgcgatcatc accggtttcg cgccctggct gatcagcggg gcgacgtgcg
     1741 tcctgcgccg aaagttctcg gcgtcgggct tccttcccga cgtccgccac tatggcgtca
     1801 cctacttcaa ctacgtcggc aagccgctgt cgtacatctt ggcgacaccc gagcgtcccg
     1861 acgacgcgca gaacacgctg acccgcgtgt tcggcaacga ggccgccgat ctcgacatcg
     1921 accggttctc ccggaggttc gcggttccgg tcgtcgacgg atacgggtcg acggaaggcg
     1981 gcgccaacgt gaaccgcacc cccgagactc cgaagggggc cttgggcgtc gcacccgacg
     2041 ggaccgtgat cctcgattcg gagaccggtg aggagtgccc ccgggcccgc ttcgacggcg
     2101 acggtcacct cctgaaccca gaggaggcga tt
//
LOCUS       DATCCH010000072         2003 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694 k141_4150,
            whole genome shotgun sequence.
ACCESSION   DATCCH010000072 DATCCH010000000
VERSION     DATCCH010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4150"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..>2003
                     /gene="gcvP"
                     /locus_tag="VIJ44_01485"
     CDS             132..>2003
                     /gene="gcvP"
                     /locus_tag="VIJ44_01485"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020548706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEY5164600.1"
                     /translation="MTLRDLEADFADRHIGPDADEQAAMLDALGLASLDELADRAVPA
                     SIRDRDPLDLPPAGDETTVLDELRALADRNTVFVSLLGGGYADTITPPVILRNVLENP
                     AWYTAYTPYQPEISQGRLEALLNFQTMVSDLTGMELANASLLDESTAAAEAMTLCHRV
                     HPKAGDTFVVDVDCHPQTIAVVSTRAEALGLDMVVAPADEFVGEGVFGVLLQEPGTSG
                     RVRDRRELIADWHARGALVAVATDLLACALLVPPGEHGADVVIGSSQRFGVPLGFGGP
                     HAGFLATRDEYKRSLPGRLVGVSVDDAGRRALRLALQTREQHIRRERATSNICTAQVL
                     LAVIAGLYAVYHGPDGIRRIARRVHRLTVLLDAGLRHRGVDVVHDDFFDTLAVRVPGR
                     AAAAAAQARERGINLRVVDDDTVGISLDETTTPAIVEAVWASFGVDARVEDLDRSTSD
                     AFPDRLARSSPFLTHPVFHRYRSETELLRYLRRLADRDLALDRTMIPLGSCTMKLNAT
                     TEMLPITWPEFARLHPFAPLDQAAGYLELFADLERWLAEITGYDAVSLQPNAGSQGEL
                     AGLLAIRAYHRDQGDDRRDVCLIPASAHGTNAASATMAGMRVMVVGCDDDGNVDLDDL
                     "
BASE COUNT          286 a          774 c          660 g          283 t
ORIGIN      
        1 aaggggaaag ccggtccgcc ggcgaatctc tcaggtccac tgacagcgcg ggagccacgg
       61 gccgagcctg gcccgagcga ccgaacgcga cccggccccc gggccggccc accagggacg
      121 gagagacggc catgacgttg cgagacctcg aggccgactt cgctgaccgt cacatcggcc
      181 ccgatgccga cgagcaggcc gccatgctcg acgcgctcgg actcgcatcc ctcgacgagc
      241 tcgccgaccg ggcggtgccg gcatcgatcc gcgaccgcga cccgctcgac ctgcccccag
      301 cgggcgacga gaccaccgtc ctcgatgagc tgcgcgccct cgccgaccgc aacaccgtgt
      361 tcgtctcgct gctcggcggt gggtacgccg acaccatcac ccctccggtg attctgcgca
      421 acgtgttgga gaaccccgcc tggtacaccg cgtacacgcc gtaccagccc gagatctccc
      481 agggccgcct cgaggcgctc ctgaacttcc agaccatggt cagcgacctc accggcatgg
      541 agctcgccaa cgcgtcgctg ctcgacgaat cgaccgccgc ggcagaggcc atgacgctgt
      601 gtcaccgggt gcaccccaag gccggcgaca cattcgtcgt cgacgtcgac tgccatcctc
      661 agaccatcgc cgtcgtgtct acccgcgccg aggctctcgg tctcgacatg gtcgtcgccc
      721 ccgccgatga gttcgtcggc gagggcgtgt tcggcgtgct cctccaggag ccgggcacga
      781 gcggccgggt ccgcgaccgg cgcgagctga tcgctgactg gcacgcccgc ggggcgctgg
      841 tcgccgtcgc caccgacctg ttggcgtgcg cgctcctcgt cccgccgggg gagcacggcg
      901 ccgacgtcgt gatcggctcg tcacagcggt tcggggtgcc actcggcttc ggtggccccc
      961 acgccgggtt cctcgcgacg cgcgacgagt acaagcgcag cctccccggc cggctcgtgg
     1021 gcgtgtccgt cgacgatgcc ggccgccggg cgttgcgcct ggcgttgcag acccgcgagc
     1081 agcacatccg ccgcgagcga gccacgagca acatctgcac cgcgcaggtg ctgctggcag
     1141 tcatcgccgg cctctacgcc gtctatcacg ggcccgacgg gatccgccgc atcgcccgtc
     1201 gagtgcaccg gctcaccgtg ctcctcgacg cggggttgcg ccaccgcggc gtcgacgtcg
     1261 tccacgacga cttcttcgac accctggccg tccgggtccc aggacgggcc gcggccgccg
     1321 cggcccaggc tcgggagcgg gggatcaacc tgcgagtcgt cgacgacgac accgtcggga
     1381 tctcgctcga cgagaccacg accccggcga tcgtcgaggc ggtctgggcc agcttcgggg
     1441 tcgacgcccg cgtcgaggat ctcgaccggt cgacgtccga cgcgttcccg gaccggttgg
     1501 cacgttcgtc gccgttcctg acccatccgg tgttccaccg gtaccggtcc gagaccgagc
     1561 tgctgcgcta tctgcgtcgc ctcgccgacc gggacctggc cctcgaccgg acgatgatcc
     1621 cgctgggctc gtgcaccatg aagttgaacg ccaccaccga gatgctcccg atcacctggc
     1681 ccgagttcgc gcgcctgcac ccgttcgcgc cgctcgacca ggcggccggc tacctcgagc
     1741 tcttcgccga cctcgagcgc tggctggccg agatcaccgg gtacgacgcg gtgtcgctcc
     1801 aacccaacgc cgggtcccaa ggcgagctgg cggggctgct ggccatccgc gcctaccacc
     1861 gcgaccaggg cgacgaccgc cgcgacgtgt gcttaatccc ggcgtcggcg cacggcacca
     1921 acgcggccag cgccacgatg gccgggatgc gcgtcatggt cgtcggctgc gacgacgacg
     1981 gcaacgtcga tctcgacgac ctc
//
LOCUS       DATCCH010000073         3216 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_211486, whole genome shotgun sequence.
ACCESSION   DATCCH010000073 DATCCH010000000
VERSION     DATCCH010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211486"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            265..1101
                     /locus_tag="VIJ44_01490"
     CDS             265..1101
                     /locus_tag="VIJ44_01490"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HEY5164601.1"
                     /translation="MARLGSLYERLCLPVAVFMLFSSQRLHPGYGMTWRKRTVLGLRI
                     YRNWRHVRTGVSYKAHLAMAAKLLELPPTVEGAVVECGSWLGGTTTNLSVICDIVGRE
                     LIVYDSFEGLPAPEAADNMQPAVRGSYKGSLETVQGNVREYGVIDRCTFRKGWFKDTL
                     PGHSEPIALCFIDVDLKSSMHDCIVNLWPHLAVQGYLFFDEYIHLDNCALFFSERFWR
                     DYLDTKPPGLMGTGTGVGVGQYFLGPWMGLSLPPIQRPTSVAYTRKGFNGLWDYVPET
                     SP"
     gene            complement(1418..1603)
                     /locus_tag="VIJ44_01495"
     CDS             complement(1418..1603)
                     /locus_tag="VIJ44_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164602.1"
                     /translation="MTDANEIATLTACKSGAEWLLAASAHMSYGVDHCIVCGKGTPRA
                     VLHAFCGGEPTTPACSK"
     gene            1918..2166
                     /locus_tag="VIJ44_01500"
     CDS             1918..2166
                     /locus_tag="VIJ44_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164603.1"
                     /translation="MLEERPKLLRLYYGDQIAAYLADLDVDAVWEAATKSERRTLTDE
                     MIEAVDVHDHLEVTVRGAPKLNQGSRSSSDKTRLIPPA"
     gene            2197..2538
                     /locus_tag="VIJ44_01505"
     CDS             2197..2538
                     /locus_tag="VIJ44_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012181420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY5164604.1"
                     /translation="MARAATTSDVFNAIAEPQRREILVLLRAGERPVTELARELGMAQ
                     PGASKHLRVLREVGLVRARKAGKQSLYGLDARGLRPVHEWTGGFERFWNESFDRLDAY
                     VQDLKQARQEE"
     gene            2542..3048
                     /locus_tag="VIJ44_01510"
     CDS             2542..3048
                     /locus_tag="VIJ44_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019068356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY5164605.1"
                     /translation="MSATGRGTPAQSGTADREIVISRVISAPRELAFEAFTEVRHVSR
                     WWGPEGFTTTTRAFEFRVGGEWDFVMHGPDGTDYQEWICWTEIAPPERIALLHGESRG
                     DPNAFESVLTFAPDGAATRIEMRTVFPTKELRDEAVEKYHAIEGGQQTLSNLAAYVTE
                     IVRKGAEG"
     gene            3048..>3216
                     /locus_tag="VIJ44_01515"
     CDS             3048..>3216
                     /locus_tag="VIJ44_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018798608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEY5164606.1"
                     /translation="MAGEVFFSVSMSLDGFIAPGSLGDLMGQQWMELQQWIFPQRFFR
                     ENLKLGEGGEEG"
BASE COUNT          566 a         1004 c         1079 g          567 t
ORIGIN      
        1 cgtgacggtg tggatgtcgt cgtgtcttca ccgtagagct catgggaaca tgcgccgccg
       61 gattggctgt cggagggcgg cgatcgcctt cgggtgtacc gctgttcacg ggcgggcggc
      121 cagtctccga gaaacatcgt ccccgttatg ctcgtgcgga tgacgggcgg tgacgaggaa
      181 acagatactt ccatgcgacg ctcggggagg tggaaggggc ccaagctcat gggagaggga
      241 cgtgacgcac gtagtgcgaa gcgaatggcg cggctcggct cgctctacga gcggctctgt
      301 cttccggtgg cggtgttcat gttgttctcg agccaacgcc ttcatcccgg ctacgggatg
      361 acgtggcgga agaggaccgt gctcggtctt cgtatctatc ggaactggcg gcacgtccgg
      421 accggcgtct cctacaaggc ccatctcgcg atggctgcga agctattgga gctccctcct
      481 acggtcgagg gtgcagtggt cgagtgtggg tcgtggttgg gtggtacgac gacgaatctc
      541 agcgtcatct gtgacatcgt cggacgcgaa ctcatcgtct acgactcgtt cgagggactt
      601 cccgcgcccg aggccgcaga caacatgcag ccggcggtga ggggctccta caagggctcg
      661 ctcgagacgg tgcagggcaa tgttcgggag tacggcgtca tcgaccgctg tacctttcgc
      721 aaaggctggt tcaaagacac cctgccgggc cattccgagc cgatcgccct ttgcttcatc
      781 gacgtggatc tgaagtcgag catgcacgac tgcatcgtga atctctggcc gcacctcgcc
      841 gttcagggct atctgttctt cgacgagtac attcatcttg acaactgcgc gctgttcttc
      901 tccgaacgct tctggcgtga ctacctcgat accaagccgc ccggactcat gggcaccggg
      961 acgggcgtcg gggtcgggca atactttctc ggtccatgga tgggtctgtc gttgcctccg
     1021 atccaacggc caacaagcgt cgcctacacg cgcaagggct tcaacggact ctgggactac
     1081 gtccccgaaa cctcgccgta gcaccactcc gagggcggcg cggagctgca tctgatattc
     1141 gcgctgacaa ctcgaacaga cccacaggtc gcttcgccgt cttgagcgaa gctgcctacc
     1201 gcacccttca cgacgaccgt gtgactcgtc atcttggtgc gttgagcctg cccggcagga
     1261 gttcgatgca cagcgacgcc cgaccgaggg ctctgacccg accgaccgtg agttccacgc
     1321 tcgaaagcac gccggaggtt ttgcagctcg ccgaacgcgc gcggacgcca ttcgttcacg
     1381 cgcgccttct atcctctcgc acggggtctc agcaccacta cttgctgcac gcgggtgttg
     1441 tcggctcgcc gccacagaac gcgtgcagta cggctcgcgg cgttcccttg ccgcacacga
     1501 tgcaatggtc caccccgtaa ctcatgtgag ccgacgcggc gaggagccat tccgctccgc
     1561 tcttacatgc cgtgagtgtc gcgatctcgt ttgcgtccgt caccgcgatc gtcgcgttcg
     1621 ggtcttcatt aacgaccgta agcatcgccg cattgcacgc tctcgagacg gttgcgggag
     1681 ccgtcgaggc ggttgtggga gtcgacgtgc tctttctccc cgaggagcat cccgccacaa
     1741 ccgcgagcca gaccactagc gccacgagca cgcgcttctt cacactcctc cccccgtcgc
     1801 gtcgggtgaa ggtatcggac caagactcct tttcacgcgc gagctagcgg ccggcgagcg
     1861 ggagcccgag agaggcgggg gtcggcgggt gcggaccctt cggtcaccct cacggcgttg
     1921 ctcgaggaac ggccgaagct gttgcgcctg tactacggcg accagatcgc cgcctacctc
     1981 gccgacctcg atgtggatgc ggtctgggag gccgcaacga agagcgaacg acgaacgctc
     2041 accgacgaga tgatcgaagc cgtcgacgtg cacgaccacc tcgaagtcac cgtacgaggc
     2101 gcaccaaagc tgaaccaagg gtcacgatcg tcatctgaca agacccgcct gatacctccg
     2161 gcttgacgca tcccgatatc ggcatatgat ggcaccatgg cacgagcagc gacgacgtcg
     2221 gacgtcttca acgcgatcgc cgagccgcag cgccgggaga tcctggtact gctgcgggcg
     2281 ggcgagcggc cggtgaccga gttggcccgg gagctgggga tggcccagcc gggggcgtcc
     2341 aaacacctgc gggtgctccg ggaggtcgga ctggtgcgcg cccgcaaagc aggcaagcaa
     2401 tctctgtacg gccttgacgc ccgcgggctg cgaccggtcc acgagtggac cggcgggttc
     2461 gagcggttct ggaacgagag cttcgaccgg ctggacgcgt acgtgcagga cctcaagcag
     2521 gcaaggcagg aggagtagct gatgagcgcg acgggacgag gaacgccggc gcagtcaggg
     2581 acggccgacc gcgagatcgt gatctcccga gtcatcagtg ccccacggga gctggcgttc
     2641 gaggcgttca ccgaagtccg gcacgtgtcg cgatggtggg gaccggaggg gttcaccacc
     2701 accacgcggg cgttcgagtt ccgcgtcggc ggggagtggg acttcgtgat gcacggaccg
     2761 gacgggacgg actaccagga gtggatctgc tggaccgaga tcgccccgcc ggagcggatc
     2821 gcgctgttgc acggtgagtc ccgcggcgac ccgaacgcct tcgagtcggt cctcacgttc
     2881 gcgcccgacg gcgcggcgac ccggatcgag atgcgcacgg tgttccccac caaggagctg
     2941 cgcgacgagg cggtcgagaa gtaccacgcg atcgagggtg gccagcagac cctgagcaac
     3001 ctggctgcct acgtcaccga gatcgttcgg aagggagcgg agggctgatg gccggggagg
     3061 tgttcttcag cgtgtcgatg tcgctggacg gtttcatcgc gcccgggtcc ctcggggact
     3121 tgatggggca gcagtggatg gaactgcagc agtggatatt cccgcagcgg ttcttccggg
     3181 agaacctgaa gctcggcgag ggcggcgagg aagggc
//
LOCUS       DATCCH010000074         2775 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_547453, whole genome shotgun sequence.
ACCESSION   DATCCH010000074 DATCCH010000000
VERSION     DATCCH010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547453"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="VIJ44_01520"
     CDS             <1..667
                     /locus_tag="VIJ44_01520"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEY5164607.1"
                     /translation="TYSVNTVWAQVAESLGTGTMTKYMKRFGFYAKPPIDLPPDETHA
                     SYPVSPTGHPYAPGSSNEDIGRIAIGQGGLEVTPLQMAMVASAVADGGRLMKPHLATR
                     AVDKDGRTVKTISPTVYHQVMKRSTAAAVAEMMRKVVDEGTGTSAQLGSGITFAGKTG
                     TASVGIAGANQTQPWFIGFAPIDNPKVAIAVTIERTDGGFGGTVAAPIAKAVVQTLLA
                     DGK"
     gene            681..2705
                     /gene="pknB"
                     /locus_tag="VIJ44_01525"
     CDS             681..2705
                     /gene="pknB"
                     /locus_tag="VIJ44_01525"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEY5164608.1"
                     /translation="MPEPTRDTMVDGRYRVVSRLGAGGMADVFLAEDQQLGRKVALKL
                     LHRRFAEDPDFVERFRREAQAAAGLQHPNVVGIYDRGEYDGTYYIAMEYLPGRTLKQV
                     IREEAPLDPIRAINIAIQILKAARFAHRRGIIHRDLKPHNVMIDDAGDAKVTDFGIAR
                     AGASDMTETGSIMGTAQYLSPEQAQGHSVSASSDLYSVGVVLYEMLTGRVPFDADAAV
                     TIALKHVSEAPVPPSAINPAIPPELEQTVLWTLNKNPADRPVDADQLITVLEHCRASI
                     EAGGAGQNTASMAAVAAGAAAGLPTAGALAEAAAAYEAAAAASDARNGTGEHATGEEG
                     EPERQRAAWVPWAWGLLVALLVAGAATAAYFISRPKHYPVPRVTGLQLNVARTLIQNA
                     HFQVDVINVESKKPTGTVIGENPGAGTKADKGSTVTLTVSRGQGNVSVPSVQGLSQTA
                     ATNVIKRVGLKVTHVVTQSSPTFKTGEATGTNPGAAKSVPPGTGVTLLVSSGQPQKAV
                     PDVRGESQSAATSQLTGAGFNVNPTTQTSSSATAGNVISQDPAGGAQATAGSTVTIVV
                     AAAPATVNVPSVTGDPTGSAFSALQSAGFHVVRQTRDVSDSSKNNEVISQSPPGGSSQ
                     KKGSTVTIVVGHYVPSSSSSSSSSSSTTTTTTSKSSSTPTTPTTSTNQTH"
BASE COUNT          475 a          985 c          939 g          376 t
ORIGIN      
        1 cacgtactcg gtcaacaccg tatgggccca ggtcgccgaa tcgctcggca ccgggacgat
       61 gaccaagtac atgaagcgct tcggcttcta cgccaagccg ccgatcgacc tgccgcccga
      121 cgagacgcac gcgagctatc cggtatcccc gacggggcat ccgtacgcgc ccggcagctc
      181 caacgaggac atcggccgga tcgcgatcgg tcagggcggc ctcgaggtca cacctctgca
      241 gatggcgatg gtcgcctcgg cggtggccga cggcggacgg ctgatgaagc cgcatctggc
      301 gaccagggcg gtcgacaagg acgggcgcac ggtcaagacg atctcgccga ccgtgtacca
      361 ccaagtaatg aagcgctcga ccgccgcggc ggtcgctgag atgatgcgca aggtggtcga
      421 cgagggcacc ggcacctctg cccagctcgg ctcgggcatc acgttcgccg gcaagaccgg
      481 gaccgcgtcg gtcgggatcg ccggcgccaa ccagacccag ccctggttca tcggctttgc
      541 cccgatcgat aaccccaagg tcgcgatcgc ggtgacgatc gagcgtacgg acggcggctt
      601 cggggggacc gtggccgcgc cgatcgccaa ggcggtcgtc cagaccctcc tggcggacgg
      661 taagtgaggc cgtatcgctg atgcccgaac cgacgcgcga cacgatggtc gacgggcgct
      721 accgggtcgt gtcacggctg ggcgcgggcg gcatggccga cgtgttcctg gccgaggacc
      781 aacaactggg acgcaaggtt gcgctcaagc tgctccatcg ccgcttcgcg gaggaccccg
      841 acttcgtcga gcgcttccgc cgggaagccc aggcggccgc cggcctgcag catcccaacg
      901 tggtcggcat ctatgaccgc ggcgagtacg acggcaccta ctacatcgcg atggagtacc
      961 tgccggggcg cacgctcaag caggtgatcc gcgaggaggc gccgctcgat ccgatccgcg
     1021 cgatcaacat cgcgatccag atcctcaagg cggcccgctt cgcccatcgc cgcggaatca
     1081 ttcaccgcga cctcaagccc cacaacgtga tgatcgacga cgccggcgat gcgaaggtca
     1141 cggacttcgg gatcgcccgc gccggcgcgt cggacatgac cgagaccgga tcgatcatgg
     1201 gcacggccca gtacctgtcg cctgagcagg ctcagggcca ttcggtcagt gcctcctccg
     1261 acctgtactc ggtgggcgtc gtgctctacg agatgctcac cgggcgcgtc cccttcgacg
     1321 ccgacgcggc cgtcacgatc gcgctcaagc acgtctccga ggcgccggtg cccccgagcg
     1381 cgatcaatcc ggcgattccc cccgagctgg agcagaccgt cctgtggacg ctgaacaaga
     1441 accctgccga tcgacctgtc gacgccgatc agctgatcac ggtgctcgag cactgccgcg
     1501 cctcgatcga ggccggcggc gccgggcaga acactgccag catggcggcg gtggccgcgg
     1561 gtgcggcggc cggcttgccg acggcggggg cgctcgccga ggccgctgcg gcctatgagg
     1621 cggccgctgc ggccagcgac gcgcgcaacg gcaccggaga gcacgcgacc ggcgaggagg
     1681 gcgagccgga gcgccagcgg gccgcctggg tgccctgggc atggggcctg ctcgtcgcgt
     1741 tgctcgttgc cggcgcggcg acggccgcct acttcatcag ccgtcccaag cactatcccg
     1801 tcccgcgcgt gaccgggcta cagctcaacg tcgcccgcac gctgatccag aacgcccatt
     1861 ttcaggttga cgtcatcaac gtggagagta agaagccgac agggacggtg atcggcgaga
     1921 acccgggcgc cggcaccaag gccgacaagg gctcgacggt caccctgacc gtgtcgcggg
     1981 gccagggcaa cgtgtcggtt ccctcggtcc aggggctcag ccagacggct gccaccaacg
     2041 tgatcaagag ggtcgggctc aaggtgaccc atgtggtgac ccagagctcg ccgaccttca
     2101 aaacgggcga ggcgaccggg accaatcccg gggccgccaa gtcggtgcct cccgggaccg
     2161 gtgtcacgct gctcgtctca tcgggccagc cgcagaaggc cgtgcccgac gtgcgcggag
     2221 agagccagtc ggcggccacg tcgcagctca ccggcgccgg cttcaacgtc aacccgacga
     2281 cccagacgtc gagctcggcg acagccggta acgtgatcag ccaggacccg gccggcggcg
     2341 cccaggcgac cgcgggctcg acggtcacga tcgtcgtcgc ggccgccccg gcgaccgtca
     2401 acgtcccgtc ggtgacgggc gacccgaccg gcagcgcgtt cagcgcgctg caatcggcgg
     2461 gcttccacgt cgtccgccag acccgggacg taagcgacag cagcaagaac aacgaggtga
     2521 tctcgcagag cccgcccggc ggctcgtcgc agaagaaggg ctcgacggtg acgatcgtcg
     2581 tcggccacta cgtgccgtcg tcctcatcgt cctcgtcctc gtcctcgagt acgacgacca
     2641 ccaccacttc gaagtccagc tcgacgccga cgacgccgac gacgtcgacc aaccagaccc
     2701 actgatccgt tgacgcgcta ctgctggcgg tgtgcggcgg ccctgcccgg acgggagggt
     2761 gaggctcctg ttatc
//
LOCUS       DATCCH010000075         2317 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_211581, whole genome shotgun sequence.
ACCESSION   DATCCH010000075 DATCCH010000000
VERSION     DATCCH010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211581"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1026
                     /gene="pncB"
                     /locus_tag="VIJ44_01530"
     CDS             <1..1026
                     /gene="pncB"
                     /locus_tag="VIJ44_01530"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012888458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0019357 - nicotinate nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0019358 - nicotinate nucleotide salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEY5164609.1"
                     /translation="EIYFPGSPVLTVRGELGQALILETLVLSVLNHDSAIASAASRMV
                     LAAGGRPLIEMGSRRTHERAAVATARAAYLSGFAATSNLAAGLLHGIPTVGTAAHAFT
                     LAHRSEKAAFASQVAVHGPGTTLLVDTYDIEAGIRTAVEVAGPELGAIRIDSGDLAEE
                     SRKARALLDSLGATKTRITVTSDLDEYLVAALADAPVDAFGVGSRVATGSGHPSAGLV
                     YKLVAVADSDDPGAPMRPVAKTAAGKVNLGGRKVAFRELGADGRAERELVTVDPSAPG
                     AVPRTWRPLQVEVMRGGSVVHRPSLAEVRAHHATALAELPGRALEIAHGEAAMSAAPA
                     PPGEVQP"
     gene            1023..1628
                     /locus_tag="VIJ44_01535"
     CDS             1023..1628
                     /locus_tag="VIJ44_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018172623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEY5164610.1"
                     /translation="MSSAFLIVDVQNDFVTGSLPVPGGADVAAAITAHLRAHPGRYAV
                     VAASQDWHSAMGDNGGHFATPGTDPDYVHTWPVHCVADEPGSAYHPALDVSLIDEHFR
                     KGQGTPAYSMFEAVDAEGHDLADALAAAGVTAVDVGGLATDYCVRATALDALAAGLHV
                     RLLTDLAAGVAPETSAAALDELAAAGVVLATTSDVRGLDSL"
     gene            1625..2314
                     /locus_tag="VIJ44_01540"
     CDS             1625..2314
                     /locus_tag="VIJ44_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015661494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY5164611.1"
                     /translation="MSDAFALTVDLAIFTIRDGAFCVLLVQRGTEPHRGRWALPGGFV
                     QPDESVEAATWRGLSAETGLPRFPGHLEQLRTYGAVGRDPRMRVVSVAHVAFAPDLPD
                     PTAGTGNDAARWWPVDEAPPLAFDHGAILSDAVERVRSKLEYTTLATRFVTEPFSLAD
                     LYLVYRAVWGVAPDLPTFRRKVLSTNGFVRPAGVSPDEALPTLLYRRGDAELLQPAML
                     RGAPDESDERE"
BASE COUNT          293 a          909 c          798 g          317 t
ORIGIN      
        1 gagatctact tccccgggtc gcccgtcctg accgtacgag gcgagctcgg gcaggcgttg
       61 atcctcgaga cgctggttct ctccgtgctc aaccacgaca gcgcgatcgc gtccgcggcc
      121 tcgcggatgg tcctcgccgc gggcgggcgg ccgctgatcg agatggggtc gcgccgcacc
      181 cacgagcggg ccgccgtcgc gacagcgcgc gcggcgtacc tgtccggctt cgccgccacc
      241 agcaacctgg cagcggggtt gctgcacggg atcccgaccg tcggcaccgc tgcccacgcc
      301 ttcacgctcg cgcaccgcag cgagaaggcg gccttcgcct cgcaggtcgc cgtgcacggc
      361 ccgggcacga cgctgctggt cgacacctac gacatcgagg cgggcatccg taccgccgta
      421 gaggtggctg gacccgagct cggcgcgatc cggatcgact cgggcgacct cgccgaggag
      481 tcgcgcaagg cccgcgcact gctcgattcc ctcggcgcca cgaagacgcg gatcacggtg
      541 acgtcggacc tcgacgagta cctcgtcgcc gcgctcgccg atgctccggt ggacgccttc
      601 ggcgtcgggt cgcgggtcgc caccgggtcg ggccatccct ctgccgggct ggtctacaag
      661 ctggtcgccg tcgcggacag cgacgacccg ggtgcaccga tgcgtccggt cgccaagacc
      721 gcagccggca aggtcaacct cggcggccgc aaggtggcgt tccgcgagct cggcgccgac
      781 gggcgcgcag agcgcgagct cgtcacggtc gacccttcgg cgcccggcgc cgtaccccgt
      841 acctggcggc cgttgcaggt cgaggtgatg cgcggcggct ccgtggtgca ccggccgtcg
      901 ctcgccgagg tccgggcgca ccacgccacg gccttggccg agctgcccgg ccgcgcgctc
      961 gagatcgccc acggcgaggc cgcgatgtca gctgcacccg caccccccgg agaggtccag
     1021 ccatgagcag cgcgttcctg atcgtcgacg tgcagaacga cttcgtcacc ggctcgctcc
     1081 cggtgcccgg cggcgccgac gtggcagcgg ccatcaccgc ccaccttcga gcgcaccccg
     1141 ggcgctacgc cgtcgtcgcg gcgtcgcagg actggcactc cgccatgggc gacaacggcg
     1201 gtcacttcgc cacgccggga accgaccccg actacgtgca cacctggccg gtgcactgcg
     1261 tcgccgacga gccggggtcg gcataccatc cggcgctcga cgtgtccctg atcgacgagc
     1321 acttccgcaa gggtcagggc acccccgcgt actccatgtt cgaggcggtc gacgccgagg
     1381 gccacgacct ggccgacgcc ctcgcggctg cgggcgtgac cgcggtcgac gtgggcgggc
     1441 tcgccaccga ctactgcgtc agggcgaccg ccctggacgc cctcgcggcc ggcctgcacg
     1501 tgcggttgct caccgacctg gcagccggcg tcgccccgga gacgtcggcc gccgcactcg
     1561 acgagctggc cgccgcgggg gtcgtgctcg cgacgacctc cgacgtgcgc gggctggact
     1621 ccctgtgagc gacgccttcg ccctcaccgt cgacctcgcg atcttcacca tccgcgacgg
     1681 cgcgttctgc gtgctgctcg tccagcgagg gaccgagccc catcgcggcc ggtgggccct
     1741 gccgggcggg ttcgtccagc ccgacgagtc cgtcgaggcg gcgacctggc gcgggctctc
     1801 cgccgagacc gggctgcccc gattccctgg gcacctcgaa cagctccgca cgtacggtgc
     1861 cgtgggccgc gacccgcgga tgcgcgtcgt ctccgtcgcg cacgtcgcct tcgcgcccga
     1921 cctgcccgac ccgacggccg gcaccggcaa cgacgctgcc cgctggtggc cggtggacga
     1981 ggcacccccg ctcgccttcg accacggcgc gatcctctcc gacgctgtcg aacgggtacg
     2041 ttccaagctc gagtacacga cgctggcgac ccgcttcgtc accgagccgt tctcgctcgc
     2101 cgacctctac ctcgtctacc gggccgtgtg gggagtggcg cccgacctcc cgacgttccg
     2161 ccgcaaggtg ctgtccacca acgggttcgt acgtccggcc ggcgtctcgc ccgacgaggc
     2221 gttgccgacg ctcctctacc gccggggcga cgccgagctg ctgcagccgg ccatgctgcg
     2281 gggcgccccc gacgagagcg acgagcgcga gtagccc
//
LOCUS       DATCCH010000076         3759 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_465496, whole genome shotgun sequence.
ACCESSION   DATCCH010000076 DATCCH010000000
VERSION     DATCCH010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465496"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="VIJ44_01545"
     CDS             <1..642
                     /locus_tag="VIJ44_01545"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEY5164612.1"
                     /translation="SDSARTAAAELDSAVGDRTLVLRVDRIDPSKNIVRGFLAYDLLL
                     ERHPEWRERVVFVARLNASREALAEYQAYRQEVETAADRVNERWATNGWTPVVLDAQD
                     NYPRTVAAMARYDVLVVNAIKDGLNLVAKEGPLVNRRDGVLCLSPGTGAWDDLRTGAL
                     AMHPFDLEQAADALHVALEMPIEERAARAVKLRALAAARTPKSWFDDQLIAAR"
     gene            complement(626..1414)
                     /gene="otsB"
                     /locus_tag="VIJ44_01550"
     CDS             complement(626..1414)
                     /gene="otsB"
                     /locus_tag="VIJ44_01550"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEY5164613.1"
                     /translation="MTLPGVLAPLVNDPGSAAIVTDFDGTLAPIVNDPTRARPLPAAV
                     SALRALVEPFGLVAVVSGRPVDFLRAQLPVDGLTLVGQYGLERLVDGDVMVDERAKAY
                     AGAVAVAADEAEHRWPRLLVERKGALAFSVHWRATPDAAPPLDEMEILARRHGLAVSR
                     GRMVGEVRAPVNIDKGAALSLLIQERRVQACAFAGDDYGDLPAFSALTDRAERQSGFV
                     GVRIAVRSPEAPPALLATADHVADSPADLAALFESLVEAVTAPR"
     gene            complement(1433..1936)
                     /locus_tag="VIJ44_01555"
     CDS             complement(1433..1936)
                     /locus_tag="VIJ44_01555"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HEY5164614.1"
                     /translation="MTAIAPAPRLGATILRTAARGAVLVGAAVVVGIVLLQVVNGTGS
                     SGGGGTTASPTPNSSTASIGTTTTLVGGGRPAAQVKVFVQNASGVSGAAATKANVLRG
                     LGYVILGQGNATTPQTGTTAGCKTGFTKEADALAKSVGVGTTVGAYPTPPPATATAAD
                     CIVTLGK"
     gene            complement(1933..2214)
                     /locus_tag="VIJ44_01560"
     CDS             complement(1933..2214)
                     /locus_tag="VIJ44_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012895284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3263 domain-containing protein"
                     /protein_id="HEY5164615.1"
                     /translation="MRGHTRSPMELSARERAILDFERSWWCFPGPKESAIRARLQVST
                     SHYYRVLSELLEKPAASEYDPLTVKRLRRYRDDRRRSRPDGRRADPGTR"
     gene            complement(2348..3085)
                     /locus_tag="VIJ44_01565"
     CDS             complement(2348..3085)
                     /locus_tag="VIJ44_01565"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HEY5164616.1"
                     /translation="MTAVVRPIECGWLSCDLGTMIAGRSGAFRFPVPAFLVEHPRGLV
                     LFDTGLHPALVTSRERLRSVADIFTPEVTEAEVVSARLRTMGIDPADIAVVVSSHLHF
                     DHCGGHALVPNARVVVQRTEWDAAHDQGMIQFGGYNPDDFEVGHDVQLLDGDHDLFGD
                     GALRLVLTDGHTAGHQSLIVDDRLVLVGDACYCRLALDTDGLPPFMADEARQRAAFAW
                     LRAQETAGRELIFSHDPDQWAALPATL"
     gene            complement(3478..3550)
                     /locus_tag="VIJ44_01570"
     tRNA            complement(3478..3550)
                     /locus_tag="VIJ44_01570"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3515..3517),aa:Thr,seq:tgt)
     gene            complement(3563..>3759)
                     /locus_tag="VIJ44_01575"
     CDS             complement(3563..>3759)
                     /locus_tag="VIJ44_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HEY5164617.1"
                     /translation="ISDIAVADRPLILVFGAEGAGLARLARARCDLLARIPMHGPIES
                     LNVAAAAAIACFEVARHRAK"
BASE COUNT          604 a         1261 c         1357 g          537 t
ORIGIN      
        1 tccgactcgg cccggaccgc cgccgcggag ctcgactcgg cggtcggaga ccgcacgctg
       61 gtcctgcgcg tcgatcggat cgacccgtcc aagaacatcg tccgcggctt ccttgcgtac
      121 gacctgctgc tggaacgtca cccggagtgg agggaacggg tcgtgttcgt cgcccgcctg
      181 aacgcgtcgc gcgaagccct cgccgagtac caggcctacc ggcaagaggt cgagacagcc
      241 gccgatcggg tcaacgagcg gtgggcgacg aacggttgga cgcctgtcgt gctcgacgcc
      301 caggacaact acccgcgcac cgtggccgcg atggctcgct acgacgtgct cgtcgtgaac
      361 gcgatcaagg acgggctcaa cctcgtcgcc aaggagggcc ctctcgtcaa ccgccgcgac
      421 ggtgtgctgt gcctctcgcc cggcaccggc gcgtgggacg atctgaggac cggagcgtta
      481 gccatgcacc cgttcgatct cgagcaggcc gcggacgcgc tccacgtcgc gttggagatg
      541 ccgatcgagg agcgggccgc ccgggccgtg aagctgcgag cactcgcggc ggcgcgcact
      601 ccgaagtcct ggttcgacga tcaactcatc gcggcgcggt aaccgcctca acgagcgact
      661 cgaatagggc ggcgagatcg gcggggctgt cggcgacatg gtcggcagtg gcgagcagtg
      721 ccgggggcgc ctccggtgac cgcaccgcga tccgcacgcc cacgaacccg gactgacgtt
      781 cagcccgatc ggtcagggcc gagaacgccg ggagatcccc atagtcgtca ccagcgaagg
      841 cgcacgcctg gacccgacgt tcttggatca ggaggctcaa cgcggcgccc ttgtcgatgt
      901 tcaccggggc gcgcacctca cccaccatcc ggccccggga caccgccaga ccgtgccggc
      961 gggcgaggat ctccatctcg tcgagcggcg gggcagcgtc gggcgtcgct cgccagtgca
     1021 ccgagaacgc cagggctccc ttccgctcga cgaggaggcg aggccaccgg tgctcggcct
     1081 cgtcggcagc gacggcgacc gcccccgcgt acgccttggc ccgttcgtcg accatgacgt
     1141 caccgtcgac gaggcgttcc aggccatatt ggcccacgag ggtcaggccg tcgacgggga
     1201 gttgggcgcg aaggaagtcg accgggcgtc cgctgaccac cgcgaccagc ccgaacggct
     1261 cgacgagggc ccgcaaggcc gacactgcgg ccggcagcgg ccgggcgcgg gtcgggtcat
     1321 tgacgatcgg tgcgagggtg ccgtcgaagt cagtgacgat ggcggccgag cctggatcgt
     1381 tgacgagggg tgcgaggacg ccgggcagcg tcatgggcgc gcggcccgct cgctacttgc
     1441 cgagggtgac gatgcagtcc gcggcggtgg cggtggccgg aggcggcgtc ggataggcgc
     1501 cgacggtggt gcccaccccg acggacttgg cgagcgcgtc cgcttccttg gtgaacccgg
     1561 tcttgcaccc ggcggtggtg ccggtctggg gtgtggtcgc gttcccttgc cccaagatca
     1621 cgtagccgag tccacggagc acgttggcct tcgttgccgc cgcgccgctc accccggagg
     1681 cgttctggac gaagaccttc acctgcgccg cgggacgacc cccgccgacc agcgtcgtgg
     1741 tcgtcccgat gctcgcagtc gacgagtttg gtgtcgggct ggccgtcgtg cccccgccac
     1801 ccgaggagcc agtgccgttg acgacctgga gcagcacgat gccgacgacg acggcggcgc
     1861 cgacgagcac cgcaccgcgc gccgcggtgc gcaggatcgt ggcgccgaga cgaggcgccg
     1921 gcgcgatggc tgtcatcgtg tgcccggatc ggcgcgtcga ccgtcggggc ggctgcgacg
     1981 ccgatcatcg cggtagcggc ggagacgttt cacagtgagg gggtcgtact cggacgcggc
     2041 gggcttctcg agcagctcgc tgaggacccg atagtaatga ctcgtcgaga cctgaagccg
     2101 agcgcggatc gctgattcct tcggacccgg gaagcaccac caggaacgtt cgaaatcaag
     2161 gattgcgcgc tcgcgcgctg agagctccat cggtgaacga gtatggcccc gcatcaccaa
     2221 aaagccgggt atttcgccca atcacgcacg aaggacgagc cccttgtacc gggactcgac
     2281 gccgattcgc gcacaaaccg gtccgcgaca ctccccgtga ccagcgtcac gcctcgacgt
     2341 cggttcttca gagcgtggcg gggagcgcag cccactggtc gggatcgtgc gagaagatca
     2401 gctcccgacc ggcggtttcc tgggctcgga gccacgcgaa cgcggcgcgt tgacgtgctt
     2461 cgtccgccat gaacggcggc agcccatcgg tgtcgagggc gaggcggcag tagcaggcgt
     2521 cgccgacgag aaccaggcgg tcatcgacga tgagcgactg gtgtcccgcg gtgtgcccgt
     2581 cggtgagcac gagacgcaac gcgccgtcgc cgaacaggtc atggtcgcca tcgaggagct
     2641 ggacgtcgtg acccacctcg aagtcgtcag ggttgtatcc gccgaactgg atcattcctt
     2701 gatcgtgggc ggcatcccac tccgttcgct gcacgacgac gcgggcgttc gggacgagcg
     2761 catggccacc gcagtggtcg aagtgcaggt ggctgctcac gaccacggcg atgtccgccg
     2821 ggtcgatgcc catggtgcgc aggcgcgcgg agaccacttc ggcctcggtc acctcggggg
     2881 tgaagatgtc cgcgacgctg cgaagccgct cccgggaggt cacgagcgcg ggatggaggc
     2941 cggtgtcgaa caggacgagg cctcgggggt gttcgaccaa gaacgcgggg acggggaagc
     3001 ggaaggctcc cgaccggccg gcgatcatcg tgccgagatc acacgagagc cacccacact
     3061 caatgggacg gacgaccgct gtcatcgggg cagcgtaaac gtcggcgccg ggctcggtca
     3121 cccggtcctc gtggtcgacc gcgctgccgg tacgaggcgg tggtcgggag cgagctgcgc
     3181 tacaggagct tcgaggcgaa ccggcgcggc ccgtgaccga ccgagttggc gcgggcacga
     3241 cggtcgtcgg tgaacgcgcg ccggcgaagt cgctcacggc gagcggcgcg cggtgcttac
     3301 cgcgccgggc gttcgccacg gcgttcgccg ctagccatcc gagccgtggg cgtcgaagac
     3361 gcttggacgg gtaccacgtt gtcacaacgc ggtcggtgta cccaacgcga atccctggct
     3421 caagcatgcg tggcagagat gcccgtcggc tggctcgtcg aggagcggcg acatcagagc
     3481 cgggtgtcgg gatcgaaccg acgacctctg ctttacaaga gcagcgctct gccagctgag
     3541 ctaacccggc gcacgattca ggctacttgg cacggtgccg cgcgacctcg aagcacgcga
     3601 tcgccgcggc cgcggccacg ttgagtgact cgatgggtcc gtgcatcggg atgcgcgcca
     3661 ggaggtcgca gcgcgctcgg gcgagtcggg ccagcccggc tccttcggcg ccgaacacga
     3721 gtatcagcgg ccggtcggcg accgcgatgt cgctgatca
//
LOCUS       DATCCH010000077         2008 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_585067, whole genome shotgun sequence.
ACCESSION   DATCCH010000077 DATCCH010000000
VERSION     DATCCH010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585067"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..1293
                     /gene="cysS"
                     /locus_tag="VIJ44_01580"
     CDS             97..1293
                     /gene="cysS"
                     /locus_tag="VIJ44_01580"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEY5164618.1"
                     /translation="MTIQLFDTAERRIVAFEPGPIVRMYVCGITPYDSTHLGHAATYL
                     AYDLLARRLEDLGHEVRMVRNVTDVDDSILPKARELGVPYLELAEAELARFRADMDAL
                     GMRPPVAEPRATEAIPGMIEMIGQLLDAGHAYLTHGTVYFDVSTFPRFGELSHYSRDH
                     MLRLAAARGGRPEDPHRRDALDFVLWQPSLPDEPAWRAPFGVGRPGWHIECSVMSMAS
                     LGVPLDLHGGGTDLIFPHHECEIAQSESVTGEPFSRHWMHSAMVSYEGEKMSKSLGNL
                     VFVSDLLKTTDARAIRLALMRHHYRAGFEWYDTDLDEGTALLHRLLVAAGRPGGPDPR
                     PFAERVRAALDRDLDAPHAVEAIDDLASAVLSGGDDPSAPDVLRELGALLGVALDRPV
                     EISTGA"
     gene            1323..>2008
                     /locus_tag="VIJ44_01585"
     CDS             1323..>2008
                     /locus_tag="VIJ44_01585"
                     /inference="COORDINATES: protein motif:HMM:NF014839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HEY5164619.1"
                     /translation="MGDDITITLPDGSTRGYSKGVTLREVATSIGPRLAKAALAAAVD
                     GEPVDLDRPVDHDAAVAIITPPTPEGREVLRHSTAHVLAQAVTDLFPGARYAIGPAIE
                     DGFYYDFELPDQQRFTDDDLERIEEHMRAIVAADQPFVREELDRDEGLARFADQPFKQ
                     EIIERVDGSEVGSGAVISVYRNPRADGPEFVDLCRGPHVPSTSRLGAFKLMRVAGAYW
                     RGDEHGPVLQR"
BASE COUNT          306 a          783 c          629 g          290 t
ORIGIN      
        1 cggcatcgag gcgttcgccg ccgtgctgct cacggtgaac gtccgccacc acgggaaggg
       61 cacgctgcgc ctactgggcc tcgaggaagg cgaacggtga cgatccagct cttcgacacc
      121 gccgaacgtc ggatcgtcgc gttcgaaccc ggaccgatcg tgcgcatgta cgtctgcggg
      181 atcacgccgt acgactccac gcatctgggg cacgccgcca cctacctggc gtacgacctc
      241 ctggcccgaa ggctcgagga tctcggccat gaggtccgca tggtccgcaa cgtcaccgac
      301 gtcgacgact cgatcctccc gaaggcccgc gagctcggcg tcccgtacct cgagctcgcc
      361 gaggctgagc tcgcccgctt ccgagccgac atggatgccc tcgggatgcg cccaccggtc
      421 gccgagcccc gggccaccga ggcgatcccg gggatgatcg agatgatcgg ccagctgctc
      481 gacgccggac acgcctatct cacccacggc accgtctact tcgacgtgtc gaccttcccc
      541 cgcttcggtg agctgtccca ctactcccgt gatcacatgc tgcggctggc ggctgcccgc
      601 ggcggacgcc cggaggaccc ccaccggcgc gacgcgctcg acttcgtgct ctggcagccg
      661 tcgctacccg acgaaccggc ctggcgggcc ccgttcggcg tcggccgccc cggctggcac
      721 atcgagtgct cggtcatgtc gatggcgagc cttggcgtcc cgctcgacct gcacggcggc
      781 ggcaccgacc tgatcttccc tcaccacgaa tgcgagatcg cccagagcga gagcgtcacc
      841 ggcgaaccct tctctcgcca ctggatgcac tccgcgatgg tcagctacga aggcgagaag
      901 atgagcaagt cgctcggcaa cctcgtgttc gtcagcgacc tgctcaagac caccgacgcc
      961 cgcgccatcc gcctcgcgct catgcgccac cactaccgcg ccgggttcga gtggtacgac
     1021 accgacctcg acgaaggcac cgcgctgctc caccgcctcc tcgtcgcggc cggccgcccc
     1081 ggcggacccg accctcgccc gttcgcggaa cgggtccggg ccgcgctcga ccgcgacctc
     1141 gacgcacctc acgccgtcga agcgatcgac gacctcgcca gcgccgtcct ctccggcggt
     1201 gacgacccca gtgctccgga cgtgctgcgc gagctcggcg cgctcctcgg tgtcgccctc
     1261 gaccgaccgg tcgagatctc cacgggcgcg tgacagacgg tcaggtaccg tcgtcaccaa
     1321 ccatgggcga cgacatcacc atcaccctcc cggacgggtc gacccgcggc tactccaagg
     1381 gcgtcaccct ccgcgaggtc gcgacgtcga tcgggccgcg tctggccaag gctgctctcg
     1441 ccgcagccgt cgacggggag ccggtcgacc tcgaccgtcc cgtcgaccac gacgccgccg
     1501 tggccatcat cacccccccg acacccgagg gccgcgaggt gctgcgccac tcgaccgcgc
     1561 acgttctcgc ccaagctgtg accgacctgt tccccggggc gcgctacgcg atcgggccgg
     1621 cgatcgagga cggcttctac tacgacttcg agctcccgga tcagcagcgc ttcaccgacg
     1681 acgacctcga gcgcatcgag gagcacatgc gagcgatcgt cgccgcggac cagcccttcg
     1741 tccgcgagga gctcgaccgt gacgagggcc tcgcccgctt cgccgaccag ccgttcaagc
     1801 aggagatcat cgaacgggtc gacggctccg aggtggggag cggcgcggtc atctctgtgt
     1861 accgcaaccc ccgggccgac ggaccagagt tcgtcgacct ctgccgtggt ccccacgtcc
     1921 cgagcacgtc tcggctcggc gcgttcaagc tgatgcgcgt ggcgggcgcc tactggcgcg
     1981 gagacgagca cggcccggtc ctgcagcg
//
LOCUS       DATCCH010000078         6069 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_257640, whole genome shotgun sequence.
ACCESSION   DATCCH010000078 DATCCH010000000
VERSION     DATCCH010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6069
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257640"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1132)
                     /locus_tag="VIJ44_01590"
     CDS             complement(<1..1132)
                     /locus_tag="VIJ44_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013424206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEY5164620.1"
                     /translation="MGRKILFVTTDQQRYDALGCNGNTIARTPVIDRLAAEGIVYRRA
                     YNQNTVCMPARSTMLTGQYVRTHGVVANGIPLPTDAPSVAAHLSEHAGYRTALLGKAH
                     FEPGFDLTGRFEENARAIRGDTGPWRGFERSVQAMHSAAAVQMTPDGPQVMGPIAHYG
                     RWLEQNHPEHLTSFAPLLAALGGGDTDAPETKNNPIPREWYHTDWIADLVIDWLDTLR
                     NEEDWFCWMSFPDPHHPWDPPASESHRIDWRDLDLPPGHPGSDEDIRTVLAGKPAHWL
                     AWWEGRWKNREGGPGSFYPSVLTHDQIREINAKVHIENELIDEALGRVLQKIDQRGWG
                     PDTDVIFTTDHGELQGDYGLLFKGPFPTDSLMRLPLVWRPAPS"
     gene            complement(1132..1539)
                     /locus_tag="VIJ44_01595"
     CDS             complement(1132..1539)
                     /locus_tag="VIJ44_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HEY5164621.1"
                     /translation="MSITPNRDQFAELAAAASADDGPVVMLNLLKFKETATSGETGAA
                     SYNRYGGSVTRMVEERGGRILWSGRVDQVLIGEEAANGWDAIALVQYPSRQAFLDMVS
                     DDTYLKSHEHREGGLADTVLLACTDRLPETSPT"
     gene            complement(1721..2542)
                     /locus_tag="VIJ44_01600"
     CDS             complement(1721..2542)
                     /locus_tag="VIJ44_01600"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY5164622.1"
                     /translation="MPTVTMRDVTIAYDVAGNPDADPIVLICGCGQPALAWNVGIGPA
                     FVRAGYRVVTFDNRGVTPSSSPPAPYTVQDLVADTVGLLDHLDIASSRFVGHSMGGWV
                     AETLAIRHPERVRAAALLGSCNVATAWEKAITTVERDLARLDYDLPPLFYATDTLRYL
                     PNHELQEDRTVEAWLTLIGDQPPWPNPGRLGQYEACLRWSLDLARTEAWSSISVPCLV
                     LAFEHDIDSPPAQARAAAARIPGAHFAIIDGTSHLAPFTHPDPVAVAVLDFFGAV"
     gene            complement(2624..3685)
                     /locus_tag="VIJ44_01605"
     CDS             complement(2624..3685)
                     /locus_tag="VIJ44_01605"
                     /inference="COORDINATES: protein motif:HMM:NF024151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164623.1"
                     /translation="MVAIPTRRWRRLHATLLAGVLLVGGAAVSAGTATAATTKPGART
                     GRVASIGTFAVGQLSETFVDPSRPTNPNGTFPGASSRTLLTAIYYPTTGAPAHQATLN
                     APFNPAHGPYPLILFSHGVLARGVFYAGLLTKWASAGYIVVAPDYPLSNTNAPGGVDF
                     GRGVADVKNQPADASFVISQVLKLDKTKKLVGGTVDQQHLGAAGHSLGAITTYGLVYS
                     ACCRDKRIDAAVELSGIPAVVEPTANYFHGVNTPLLAVHGNADPIVPYQADVDGFARA
                     QPPKFFLTFLGGGHVSPFLSPTGADGLALQKTTVDFWDRYLKDDTAALGQLKTDATVP
                     GSTTFQDDPVPTTASHAKP"
     gene            complement(4087..4371)
                     /locus_tag="VIJ44_01610"
     CDS             complement(4087..4371)
                     /locus_tag="VIJ44_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEY5164624.1"
                     /translation="MIWRRLTARLTRDLACRQFVEVVTDYLEGAMPARERARFEHHLS
                     RCDGCDRYLAQIRRTLDVTGRLTVADVDALGSVARERILGAFWEFHTQGA"
     gene            complement(4368..5021)
                     /locus_tag="VIJ44_01615"
     CDS             complement(4368..5021)
                     /locus_tag="VIJ44_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018640209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY5164625.1"
                     /translation="MTSAGTDDVDDRSLVDRLRRGDEVAFAWLLDRYTGSLHRLSRSY
                     VATASVADEAVQDTWLAVITGIDRFEGRSSLKTWLHRILINIARTKGVREHRSIPFAS
                     LGSEIDGDDPAVDPARFRGGRDHHAGQWATPPVPWDEAPEDRLLSRETLATVQHAIDE
                     LPPNQRTVITLRDLEGWPSEDVCNVLAITETNQRVLLHRARSKVRGALETHFEDTTA"
     gene            5238..>6069
                     /locus_tag="VIJ44_01620"
     CDS             5238..>6069
                     /locus_tag="VIJ44_01620"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEY5164626.1"
                     /translation="MSAEGTGQDGAPPRESRRKYAVLFLALAVFAVAGVLVASQSNPN
                     SVTGPVDTSHLPVGPPLPELTDAKAWINSTPLASSDLQGKVVVYDFWTYSCVNCVRSL
                     PFLRSWYDRYRQYGLVVIGIHSPEFDFEKDHGNVATAVKNLHVDYPVALDDDMAIWNQ
                     FGNQYWPETWVADRQGRLRYQHIGEGNDATTEDVLRRLLSVPASAPRARVVNGQNLGQ
                     PPQQSQAVTQETYLGTDHGTAGVQSGLVSYPEPQGLGAGDARVVGPWLGSGQQVQAAA
                     P"
BASE COUNT          929 a         1907 c         2231 g         1002 t
ORIGIN      
        1 ctgagggcgc gggtcgccac accaggggga ggcgcatgag cgaatcagtc gggaagggac
       61 ctttgaagag gaggccgtag tcaccctgta gctcgccgtg gtcggtggtg aagatgacgt
      121 cggtgtcggg accccacccc cgttggtcga tcttttgcag gacacggccg agtgcctcgt
      181 cgatgagctc attctcgata tggaccttgg cgttgatctc gcgaatctgg tcgtgcgtga
      241 gcacgctcgg gtagaagctt ccgggtccac cctcacggtt cttccagcgt ccctcccacc
      301 aggcgagcca gtgagcgggc ttgccggcga ggacagtgcg gatgtcctcg tccgagcctg
      361 ggtgcccggg gggtaggtcg aggtcgcgcc agtcgatccg gtgcgactcg gaggcgggcg
      421 ggtcccacgg gtgatgaggg tccgggaagc tcatccagca gaaccagtcc tcttcattgc
      481 ggagggtgtc cagccagtcg atgaccaggt ccgcgatcca gtcggtgtgg taccactccc
      541 gcgggatcgg gttgttcttc gtctccggcg cgtcggtgtc accgccgccg agcgccgcga
      601 ggagcggggc gaagctggtg agatgctcgg ggtggttctg ctccagccag cggccgtaat
      661 gggcgatcgg gcccatgacc tgcgggccgt ccggggtcat ctgcaccgcg gcggcggaat
      721 gcatggcctg cacagaacgc tcgaatccgc gccacgggcc ggtgtcacca cgtatcgcgc
      781 gggcgttctc ttcgaagcgt ccagtcaggt cgaagccggg ctcgaaatgg gctttgccga
      841 gcagcgcggt gcggtagccc gcgtgctcgg acagatgggc cgcgacgctg ggcgcgtcgg
      901 tggggagggg gatgccgttg gcgacgacac cgtgggtgcg cacgtactgg cccgtgagca
      961 tcgttgagcg ggccggcatg cacaccgtgt tctggttgta ggcccgccgg taaacgatcc
     1021 cttcggccgc gagccgatcg atgaccggtg tgcgggcgat ggtgttcccg ttgcagccca
     1081 gcgcgtcgta acgctgctgg tcggtcgtga cgaagaggat cttgcggccc atcaggttgg
     1141 cgacgtctcc ggtagccggt ccgtgcaggc aaggaggacg gtgtcggcga gaccgccctc
     1201 gcggtgctcg tgcgacttca ggtacgtgtc atcggagacc atgtcgagaa acgcttggcg
     1261 ggacgggtac tgcaccaagg cgatggcgtc ccagccgttc gcagcctcct cgccgatcaa
     1321 gacctgatcg acccggccgc tccacaggat ccgaccccca cgctcctcga ccatccgggt
     1381 caccgagccg ccgtaccggt tgtaggaggc cgcgcctgtt tcgccactgg tcgcggtctc
     1441 cttgaacttc aacagattca gcatgaccac cggcccgtcg tcggcggacg cggccgccgc
     1501 cagctcggcg aactggtcac gattcggagt tatggacacg acgctctcct ctactcaggg
     1561 ccgctgcatg ttgccacgtg gtggcgatcc ccgtcgcggg gcatcgggat cctgttcttg
     1621 gggttggtgt cggcgggcgc gcgcgtgacc ttgcgcgaag ggggcgcggt gccgatcggg
     1681 gtggccgtgc ccgacggcgc gtcgaaaccg ggcgcgaggg tcagacggcg ccaaagaagt
     1741 cgagcacggc caccgccacg ggatcggggt gcgtgaacgg ggccaggtga ctggttccgt
     1801 cgatgatcgc gaagtgcgca ccggggatcc gcgccgcggc cgctcgcgcc tgggccggtg
     1861 gtgagtcgat gtcgtgttcg aaggccagca cgagacacgg gacgctgatc gacgaccacg
     1921 cttcggtacg ggccaggtcg agggaccagc gcaggcaggc ctcgtactgt cccaggcggc
     1981 ccgggttcgg ccacggtggc tggtcaccga tcagggtcag ccaggcctcg acagtgcggt
     2041 cctcttgcag ctcatggttg ggtaggtacc gcaacgtgtc ggtggcgtag aacagcggcg
     2101 ggaggtcata gtcgagccgg gcgaggtccc gctcgaccgt ggtgatcgcc ttctcccagg
     2161 cggtcgcgac gttgcagctc ccgaggagcg ccgcggctcg cacccgttcg gggtgccgga
     2221 tcgccagggt ctcggcgacc caaccgccca tcgagtgacc gacgaagcga gacgaggcga
     2281 tgtcgaggtg gtcgagcagc cccacggtgt cggccacgag gtcctgcacg gtgtacgggg
     2341 ccggtggtga cgacgagggg gtcacgccgc ggttgtcgaa ggtgacaacg cggtacccgg
     2401 cgcgaacgaa ggcgggcccg atgccgacgt tccacgccaa ggcgggctgt ccgcacccgc
     2461 agatcaggac gatggggtcg gcgtcgggat tcccggcgac gtcgtaggcg atcgtgacgt
     2521 cgcgcatcgt gaccgtgggc atccgtcccc ctctgttccc tgagactggg cgctgtggcg
     2581 agccgggtgg cctgcggtgt cgctcggccg cgcggcgcgg aggctacggc ttggcgtgcg
     2641 aagcggtggt cggcaccggg tcgtcctgga acgtggtgga ccccggcacg gtggcatcgg
     2701 tcttgagctg accgagcgcg gcggtgtcat ctttcaggta gcggtcccag aagtccacgg
     2761 tcgtcttctg caaggccaag ccgtcggcgc cggtggggct aaggaagggc gagacgtgac
     2821 cgccgccgag gaatgtgagg aagaacttgg gcggctgggc ccgggcgaag ccgtcgacat
     2881 cggcctgata cgggacgatc gggtcggcgt tgccgtggac cgcgagcagc ggggtgttca
     2941 ccccgtggaa gtagttggcg gtcggctcga cgaccgcggg gatgccggac agctcgaccg
     3001 cggcgtcgat gcgcttgtcg cgacagcacg cgctgtagac cagcccgtag gtcgtgatcg
     3061 cgccgaggga gtgaccggct gcgccgaggt gctgctggtc gacggttccg cccaccagct
     3121 tcttggtctt gtcgagcttg agcacctggc tgatcacgaa gctggcgtcc gcgggctggt
     3181 tcttcacgtc cgcgacaccc cggccgaagt ccacgccgcc gggcgcgttg gtgttcgaca
     3241 acggatagtc gggcgcgacg acgatgtagc ccgccgacgc ccatttggtg agcaggccgg
     3301 cgtagaagac accgcgggcg aggacgccgt ggctgaacaa gatgagcgga tacggcccgt
     3361 gggcagggtt gaacggcgcg ttcagcgtgg cctggtgggc gggtgcgccg gtcgtcgggt
     3421 agtagatggc ggtgagcagg gttcggctcg acgctccggg gaaggtgccg ttcgggttgg
     3481 tcggtcggct cgggtcgacg aaggtctcgc tgagctgacc gacggcgaag gtgccgatcg
     3541 aggcgacgcg ccccgttcgc gcaccaggtt tggtcgtcgc ggccgtggcg gtgccggccg
     3601 agaccgctgc gcccccgacg agcagcacac cggcgagcag ggtcgcgtgg agccgacgcc
     3661 atcgacgagt tggtatcgcg accatccgtc cccttggcgt gcccgcagga tcgggaccgc
     3721 ccgaacgcga ctctaccggc cgtcggctcg cgggcccgtc agtacgcggc ggaccgcctg
     3781 cctcgaggcg cgtggtggag ccgacgtgcg tggtggagcc gacgttcgcg gtggctcgta
     3841 tacggcggcg acgcgcggcc cgaccaggag ggtgatcgaa ggctcggtcg actcgccctg
     3901 cccgcgggac cgggcctctt agcggccggt cggtcgggtg agcgcggcgg cgccttcgat
     3961 gaccggaagc cgcaggcgag acgtcgactg gatcgtctgg cggccggacc gacacgtcgg
     4021 gtccaggcga ctcgcggtct tgagaaccgc gagtcggcca aagcgggcgg accaagttct
     4081 gcggcgttag gcgccctggg tgtggaattc ccagaaggct ccgagtatcc gttcgcgtgc
     4141 gacggacccg agagcatcga cgtcggcgac ggtcagtcgt cccgtgacgt cgagcgtccg
     4201 ccgtatctgg gccaggtagc ggtcacagcc gtcgcaacgc gataggtggt gctcgaagcg
     4261 ggcgcgttcg cgtgcgggca tggcgccctc gaggtagtcg gtcacgacct cgacgaattg
     4321 gcgacacgcg aggtcccggg tgagtcgagc ggtgagcctg cgccagatca tgccgtcgtg
     4381 tcctcgaaat gggtttccag ggcaccgcgc accttggacc tcgcccggtg aagtagcacc
     4441 cgctgattgg tctcggtgat cgccaagacg ttacaaacgt cttcggacgg ccagccttca
     4501 aggtcacgca acgtgatcac ggttcgctga ttgggtggga gctcgtcgat ggcgtgctgc
     4561 accgtggcga gcgtctcgcg ggacaggagc cgatcctcgg gggcttcgtc ccacggcacg
     4621 ggcggcgtcg cccactgccc ggcgtggtga tcgcgcccgc cgcggaaccg ggccgggtcg
     4681 actgcggggt cgtcgccgtc gatctcggaa cccagcgacg cgaacgggat gctgcggtgc
     4741 tctcgcacgc ccttggtgcg ggcaatgttg atcaagattc ggtgcagcca ggtcttcagc
     4801 gaagaacggc cttcgaaccg atcgatccca gtgatgaccg cgagccatgt gtcctgcacc
     4861 gcctcgtctg ccaccgacgc ggtggcgaca tacgaacggg acagccggtg cagtgatccc
     4921 gtatagcggt cgagcagcca cgcgaacgcc acctcatcgc cgcgtcgaag ccggtcgacg
     4981 agggacctat cgtcgacatc gtcggtgccg gcggaggtca cggtcggcat tctcacccac
     5041 ccaccagaaa agccgaggtc ggccctgtaa cgagtagccc tgggatgtca ctaagtggac
     5101 agggccgcca tccgaccggc cttgggcgac gtattggaga gggatgggac tacgaccggg
     5161 tgctgtgaac gccgtcgggc ccgtgcgttc ggtcggctcg taccgacccg ccggagctgc
     5221 gtcgtcgcga ggtcaccatg tcggctgagg ggacgggtca ggacggtgcc ccgcctcggg
     5281 agtcccggcg caagtacgcg gtgctgttcc tggcgctcgc ggtgttcgca gtagcgggcg
     5341 tcttggtggc cagccagtcg aatccgaaca gcgtcaccgg tcccgtcgac acctcccatc
     5401 tgccggtggg tccaccactt ccggaattga ccgacgccaa ggcttggatc aactcgacgc
     5461 cgctggcttc gtcggacctg cagggcaagg tcgtggtcta cgacttctgg acctattcgt
     5521 gtgtgaactg cgtgcgatcg ctgccctttc tgcggtcgtg gtacgaccgg taccgccaat
     5581 acggactggt cgtcatcggg atccactcac ccgagttcga cttcgagaag gaccacggca
     5641 acgtcgcaac tgccgtgaag aacctgcatg tcgattatcc ggtggcgctc gacgacgaca
     5701 tggcgatctg gaaccagttc ggaaatcagt actggcctga gacgtgggtc gcggaccgtc
     5761 aaggccggct ccggtatcaa cacatcggcg aaggcaacga cgccaccacc gaggatgtgc
     5821 tgcgtcgctt gctcagtgtc ccggcgtcag caccccgagc tcgggtcgtc aacggccaga
     5881 acctgggtca gccgccccag cagagccagg ccgtcaccca agagacgtac ctcgggaccg
     5941 atcacggcac cgcgggggtg cagtctggct tggtgagcta ccccgaaccg caggggctcg
     6001 gcgcgggcga cgctcgtgtc gtggggccct ggctcggcag cggccagcag gtccaggcgg
     6061 ccgcacccg
//
LOCUS       DATCCH010000079         2043 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_211740, whole genome shotgun sequence.
ACCESSION   DATCCH010000079 DATCCH010000000
VERSION     DATCCH010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211740"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..252)
                     /locus_tag="VIJ44_01625"
     CDS             complement(109..252)
                     /locus_tag="VIJ44_01625"
                     /inference="COORDINATES: protein motif:HMM:NF018140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164627.1"
                     /translation="MANLLGKRYQCAVCEATVLVTKPGDGELECHGQVMTVAQAKPLP
                     SSD"
     gene            complement(262..390)
                     /locus_tag="VIJ44_01630"
     CDS             complement(262..390)
                     /locus_tag="VIJ44_01630"
                     /inference="COORDINATES: protein motif:HMM:NF018140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164628.1"
                     /translation="MPATKVGTRLRCDRCGTEIIVVKTADDSQPVCCGQPMVAREG"
     gene            409..1233
                     /locus_tag="VIJ44_01635"
     CDS             409..1233
                     /locus_tag="VIJ44_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY5164629.1"
                     /translation="MRDPDDFSDLTFAVDDNVAVITLCRPERRNAFTGAMGESLGAAY
                     QRCDEDDSVRAVVVTGAGDAFCAGADLAPAGETFAAPETDPGKFTSSPISPPAWEVRK
                     PVLAAMNGHAIGIGMTLAMQCDLRIMATGAKYGFVHVRRGVIPDAHSHWTVPRAVGFS
                     QAADLLLTGRHFSAEEAVAMGLATRAVAPEQVLATALAVARDIATNAAPLSVAITKRL
                     LWASPPLDAAETDRLESAMHHHIMGEADAREGVLAFLERRKPDWSLSPTRHWPDAW"
     gene            complement(1245..>2043)
                     /locus_tag="VIJ44_01640"
     CDS             complement(1245..>2043)
                     /locus_tag="VIJ44_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007508027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5164630.1"
                     /translation="RPLLKDRESAEFAHPASYMFRNLPAARYAPDPLRAVLDEMDRFG
                     VERALVGVHPGHDTARRALRDHADRFIGSYDVDPNQGVGGTQALRAAVDDLGVRAATA
                     FTAGTFPQVPLDAPAWYPLYSTCVDLGLPLFVTVGIPGPRVPFAPQDVRRLDQVCYDF
                     PELVVVMRHGAEPWADLAVKLMLKWPGLHYSTSAFAPRYYPKAIVDYANTRGATKVCF
                     AGYFPMGLSYETIFEQLPSVPFRDHVWPHFLRDNALRLLGESSPRAS"
BASE COUNT          330 a          688 c          724 g          301 t
ORIGIN      
        1 cgacgaccac cgcggccgca ccgagatggt cgatgagggc agcgtcgacg gcggcacggc
       61 cggccgcgag cagccgggcg tcgacctcga cgaccggcac ggccgggttc agtcggacga
      121 gggaagcggc ttggcttggg cgaccgtcat gacctggccg tggcattcga gctcgccgtc
      181 gccgggcttg gtcacgagca ccgtggcctc gcacacggcg cattggtagc gcttcccgag
      241 caggttcgcc atggttgcct ctcagccctc gcgagcgacc atcggctggc cgcagcacac
      301 cggttggctg tcgtcggcag ttttcacgac gatgatctcg gtgccacagc ggtcacagcg
      361 aagacgggtc ccgactttcg tcgcaggcat aggggacaga ctatccgggt gcgtgatccg
      421 gatgacttct cagacctgac gtttgccgtc gacgacaacg tggcggtgat caccctgtgc
      481 cgccccgagc ggcggaacgc gttcaccggt gccatgggag agagcctcgg cgcggcttac
      541 cagcgctgcg acgaggacga cagcgtgcgg gcggtggtcg tgaccggcgc gggcgacgcg
      601 ttctgcgccg gcgccgatct cgcgcccgcc ggcgagacgt tcgccgcgcc ggagacggat
      661 ccggggaagt tcacctcgtc accaatcagt ccgccggcat gggaggtccg caagccggtc
      721 cttgcggcga tgaacggtca cgccatcggg atcggcatga cgctggccat gcagtgcgat
      781 ctccggatca tggcgacggg tgcgaagtac gggttcgtac acgtccggag aggggtgatc
      841 cccgacgcgc actcccactg gaccgtgccg cgggcggtcg ggttctccca ggccgccgac
      901 ctgttgctca ccggccgtca cttcagcgcc gaggaggccg tggcgatggg cctcgccact
      961 cgagcggtcg cccccgagca ggtcctggcc accgcgttgg cggtggcacg ggacatcgca
     1021 acgaatgccg cacccctgtc cgtcgcgatc acgaagcggc ttctctgggc cagcccgccg
     1081 ctcgacgccg ccgagaccga tcgcctcgag agcgcgatgc accaccacat catgggagaa
     1141 gccgacgcac gcgaaggtgt cctggcgttc ctcgaacgtc gcaagcccga ctggtcactt
     1201 tcgccaactc ggcactggcc cgacgcttgg tgaatcgacc ccgatcacga cgctcgcggc
     1261 gacgactccc cgaggagtcg gagcgcgttg tcgcggagga agtgcggcca cacgtggtca
     1321 cggaacggga ctgagggcag ctgctcgaag atcgtctcgt acgagagccc catcgggaag
     1381 tagcccgcga agcaaacctt cgtggccccg cgggtgttcg catagtcgac gatcgccttc
     1441 gggtagtacc ggggtgcgaa cgcgctcgtg gagtagtgca gtccaggcca cttgagcatg
     1501 agcttcaccg cgaggtcggc ccacggctcg gcgccgtggc gcatcaccac gacgagctcg
     1561 gggaagtcat agcagacctg gtcgagccga cggacgtcct gcggtgcgaa cgggacgcgc
     1621 ggccccggga tccccaccgt gacaaacagc gggaggccga ggtcgacgca ggtgctgtac
     1681 agcggatacc acgcgggtgc gtcgagggga acctgcggga aggttcccgc cgtgaacgcg
     1741 gtcgcggcgc ggacgccgag gtcgtccacg gccgcccgca gtgcctgcgt ccccccgacc
     1801 ccctggttcg ggtccacgtc gtatgacccg atgaagcggt cggcatggtc gcgcaacgct
     1861 cgccgcgccg tgtcgtgacc ggggtgcacc ccgaccagtg cccgctcgac tccgaagcgg
     1921 tccatctcgt ccagcaccgc ccgcaacggg tcgggtgcgt agcgggccgc gggaaggttt
     1981 cggaacatgt acgaggcggg gtgagcgaac tcggccgatt cgcggtcctt gagaagcgga
     2041 cga
//
LOCUS       DATCCH010000080         3937 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_507018, whole genome shotgun sequence.
ACCESSION   DATCCH010000080 DATCCH010000000
VERSION     DATCCH010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507018"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..734
                     /locus_tag="VIJ44_01645"
     CDS             87..734
                     /locus_tag="VIJ44_01645"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEY5164631.1"
                     /translation="MRRTLAEHDSLALLHDHGIPVVAETVVATPADAARAATAVGFPV
                     VVKAAGDRIAHKTERGLVRLGLRSAPEVEVAATQVLDATGPEDGAVALVVAPMVRGDR
                     ELIAGLHRDDQFGPCVMVGIGGVLAEALADVAIRLAPIDTHDAHDMLDDLRGGALLGP
                     FRGEPAVDRDRVAAVLLALSDLATARPDLVAIDVNPLVIVDGAPVAVDALVELDS"
     gene            731..2212
                     /locus_tag="VIJ44_01650"
     CDS             731..2212
                     /locus_tag="VIJ44_01650"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEY5164632.1"
                     /translation="MKPRLASERFQPLFDPRGVLVAGVSSHPGKFGFVALHNIVTQGF
                     TGPVFGTNRDGGEVLGVPMLASADDLPADAPVDLVVVCTPAVANPDLLRSCARRGITA
                     AFVTSAGYGEAGEQGRADEADLVALADDLGILLAGPNGQGLASTPSRLCAQIVAPFPP
                     PGAIGIVSQSGNFVSSFQNLARATGVGVSRAVSAGNAAAVTVPDFLEYYASDAATRVG
                     LAYVEGVGDGRAFFTRVRDVAATKPLVLLKGGTTTGGQRAAASHTGALASDVRIFDGL
                     CRQAGVTRADTVEDAFEAAATFATQPLPRGPRVAVVTTAGGWGVVTADAISRSDLELA
                     TLPDDLRVAIDERLPPRWSRNNPIDLAGGETRDTVPEILDLVTRHPDVDAVIYLGIGI
                     QSNQARLMREGRFASDPNLERIVAYHERQDARFAQAAAAVSDATGKPVLTATELAVAD
                     PANPGPRTVRDTGRLCYPSSYRAVRALEHLWHRARYQQTRGQA"
     gene            2250..3644
                     /locus_tag="VIJ44_01655"
     CDS             2250..3644
                     /locus_tag="VIJ44_01655"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEY5164633.1"
                     /translation="MTRWRITAGILLAGALVCAALALRPDSTSSSSNGTTLRTPLFSP
                     RRLPELIVDSVGAQHLQSALDADVSAQNACFLVTEGGQPRAVHNPDTPLIPASTEKLL
                     TAAAALTVLGPNFKYETKAVAATAPHGGTLDRLWIVGAGDPILATGDYLAYRQTQPTS
                     RNAIATRLEPLADAIAAQGVKQIPGGVVGDDSRYDQQRYVPSWPTSYRTDPVIGPLGA
                     LTVNGGYRLTGGRPVPVDDPALATASTLTTLLQARGIQVGPPEAHIPVPATTTPLASV
                     MSPPLRDILMFFLSSSDDLTGELLTKELAVHAGSPGTTATGVQRIAATLQTLGLPTAG
                     LTLIDGSGLDRGNRATCRLLAAALDLGLRPQDRTILDGLAVAGQDGTLAVEFNGSPLA
                     GKLHAKTGFLNGVDGLVGLLDAGRPLRFALLLNGNLTMPEAFAARERFGGILATFPSA
                     PAADLLVPAPDPAR"
     gene            3684..>3937
                     /locus_tag="VIJ44_01660"
     CDS             3684..>3937
                     /locus_tag="VIJ44_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164634.1"
                     /translation="MTPNVAGVDARLHTEPPPRSPRSRRLPPGVAAVLAATVAAIILA
                     ACGNGGNSNINLNSGGQQPATNPAAARIHTIPGMPAVVNPT"
BASE COUNT          583 a         1702 c         1147 g          505 t
ORIGIN      
        1 accgacgccg gcaagctcct caaacgggtc ctgcgcgacg aacactggcg cgcccacgac
       61 cgcgccgtct gaggccgccc cacccgatgc gccgcacgct cgccgagcac gactctctgg
      121 cgctcctcca cgatcacggc atcccagtcg tcgccgagac cgtcgtcgcc acccccgccg
      181 acgcggcccg cgccgccacc gccgtcgggt tcccggtggt cgtcaaagcg gccggggacc
      241 gcatcgccca caagactgag cggggcctcg tccggctcgg gttgcgcagc gcccctgagg
      301 tcgaagtggc cgccacccag gtcctcgacg ccaccggccc cgaggacggc gccgtcgcgc
      361 tcgtcgtcgc cccgatggtc cggggcgacc gggagctcat cgccgggttg caccgcgacg
      421 atcagttcgg accatgcgtc atggtcggga tcggcggcgt cctcgccgaa gcgctcgccg
      481 acgtcgcgat ccggctcgcg cccatcgaca cccacgacgc gcacgacatg ctcgacgacc
      541 tgcgcggcgg cgcgctgctc ggcccgttcc gcggcgagcc cgcggtcgac cgcgaccggg
      601 tcgccgcagt gctgctcgcg ctctcggacc tcgcgaccgc ccgtccggac ctggtcgcca
      661 tcgacgtcaa cccgctcgtc atcgtcgacg gggccccggt cgccgtcgac gccctcgtcg
      721 agctcgactc gtgaagccgc gcctcgcctc cgagcggttc cagccgctct tcgacccacg
      781 cggggtgctc gtcgccggcg tgtccagcca tcccggcaaa ttcgggttcg tcgcgctcca
      841 caacatcgtc acgcaagggt tcaccggacc ggtgttcggg accaaccgcg acggaggcga
      901 ggtcctcggg gtcccgatgc tcgcctccgc cgacgacctg cccgccgacg ccccggtcga
      961 cctcgtcgtg gtctgcaccc ccgccgtcgc gaacccggac ctgctgcgca gctgcgcgcg
     1021 tcgcggcatc accgccgcgt tcgtcacctc cgccgggtac ggcgaagcag gggagcaagg
     1081 ccgggccgac gaagccgacc tcgtcgcgct cgccgatgac ctcgggatcc tcctcgccgg
     1141 accgaacggc caagggctgg cctcgacccc gtctcggctc tgcgcccaga tcgtcgcccc
     1201 attcccgcca ccaggcgcga tcgggatcgt cagccagtcc ggcaacttcg tgtcctcgtt
     1261 ccagaacctg gcccgcgcca ccggcgtggg tgtcagccgc gccgtatcag ccggaaacgc
     1321 cgccgcggtc accgtccccg acttcctcga gtactacgcc agcgacgccg cgacccgcgt
     1381 cggtctcgcg tacgtcgaag gggtcggcga cggtcgcgcc ttcttcaccc gggtccgcga
     1441 cgtcgcggcc accaaacccc tcgtcctcct caaaggcggc accacgaccg gggggcaacg
     1501 cgccgccgcg tcccacaccg gcgcgctcgc cagcgacgtc cgcatcttcg acggcctctg
     1561 ccgccaagcc ggcgtcaccc gcgccgacac ggtcgaagac gccttcgaag ccgcggcgac
     1621 gttcgcgacc cagccgctcc cacgaggccc acgcgtcgcg gtggtcacca ccgccggtgg
     1681 ctggggagtc gtcaccgccg acgcgatcag ccgttccgac ctcgagctcg cgaccctccc
     1741 ggacgatctg cgcgtcgcaa tcgacgagcg gctcccgcct cgctggagcc gcaacaaccc
     1801 catcgacctc gccggtggcg agacccgcga caccgtcccc gagatcctcg acctcgtgac
     1861 ccggcatccc gacgtcgacg cggtcatcta cctcgggatc ggtatccagt cgaaccaagc
     1921 ccggctcatg cgcgaaggac gattcgcgtc cgacccgaac ctcgaaagga tcgtcgcgta
     1981 ccacgaacgc caagacgccc gcttcgcgca ggccgccgct gcggtatcgg acgcgaccgg
     2041 caaaccggtc ctgaccgcca ccgaactcgc cgtcgccgac cccgcgaacc ccgggcctcg
     2101 caccgtgcgc gacaccggcc ggctctgcta cccgtcctcg taccgcgcgg tgcgcgccct
     2161 cgagcatctc tggcaccggg cccgctacca gcagacccga ggccaggcgt gactcgcatc
     2221 ccgacgcacc ggaaacggcg ccacacacca tgacccggtg gcggatcacc gccgggatcc
     2281 tgctcgccgg cgcactggtc tgcgccgccc tcgcgctccg tcccgactcg acgtcgagca
     2341 gctccaacgg gaccacgttg cgcactccac tcttctcacc ccgacgactg cccgagctca
     2401 tcgtcgacag cgtcggcgcc caacacctcc agagcgcgct cgacgccgac gtcagcgccc
     2461 agaacgcctg cttcctcgtc accgaaggcg gtcagccccg ggccgttcac aaccccgata
     2521 cgccgttgat tcccgcgagc accgagaagc tgctcaccgc ggccgccgcg ctcaccgtcc
     2581 tcggcccaaa cttcaagtac gagacgaagg ccgtcgccgc gaccgccccc cacggcggca
     2641 ccctggaccg gctctggatc gtcggcgccg gtgaccccat cctcgccacc ggcgactacc
     2701 tcgcgtaccg ccagacccaa ccgaccagcc gcaacgccat cgccactcgc ctcgaacccc
     2761 tggccgacgc catcgccgcc caaggggtca agcagatccc gggcggggtc gtcggcgacg
     2821 acagccgcta cgaccagcag cgatacgtcc ccagctggcc gaccagctac cgcaccgacc
     2881 cggtcatcgg ccccctcggg gcgctcaccg tcaacggcgg ctatcgcctc accggcggcc
     2941 gtcccgtccc ggtcgacgac ccggccctcg ccaccgcatc aaccctgacc accctgctcc
     3001 aggcccgcgg catccaagtc ggaccccccg aggcacacat ccccgtcccc gccaccacca
     3061 cgccgctcgc gtcggtgatg tcaccgccac tgcgcgacat cctcatgttc ttcctcagct
     3121 ccagcgacga cctcaccggc gagctcctca ccaaggaact cgcggtccac gccggcagcc
     3181 ccggcaccac cgccaccggg gtgcagcgga tcgccgccac gctccagacc ctcggcctcc
     3241 cgaccgccgg tctcaccctc atcgacgggt ccggcttgga ccgcggtaac cgggccacct
     3301 gccggctcct cgccgcggcc ctcgacctcg gcctccgacc ccaagaccgg accatcctcg
     3361 acggactcgc cgtcgccgga caagacggca ccctcgcggt cgagttcaac ggcagcccgc
     3421 tcgcgggcaa gctccacgcc aagaccgggt tcctcaacgg cgtcgatgga ctcgtcggcc
     3481 tcctcgacgc cggacggccg ctgcgattcg cgctcctctt gaacggcaac ctcaccatgc
     3541 ccgaagcgtt cgccgcccgc gaacgcttcg gtggcattct cgccaccttc cccagcgccc
     3601 ccgccgccga cctgctcgtc cccgcacccg acccggcccg ctgacgccag cttgttgaag
     3661 gtcccctcct aggatcccga cccttgaccc caaacgtcgc cggcgttgac gcgcgcctcc
     3721 acaccgaacc gcctccccga tccccccgat cccgtcgcct cccacccggc gtcgccgcgg
     3781 tcctcgccgc gaccgtcgcc gcgatcatcc tcgccgcctg cgggaacggc gggaactcga
     3841 acatcaacct caactccggc ggccagcaac cggccacgaa cccggccgcg gcccggatcc
     3901 acaccatccc gggcatgccc gcagtcgtga accccac
//
LOCUS       DATCCH010000081         2908 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_211891, whole genome shotgun sequence.
ACCESSION   DATCCH010000081 DATCCH010000000
VERSION     DATCCH010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211891"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="VIJ44_01665"
     CDS             <1..504
                     /locus_tag="VIJ44_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015036192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEY5164635.1"
                     /translation="IPVGNAGNITAYWRGYCEDAEAGRATRRPRMLGWQATGAAPIVR
                     GEPVLHPETLATAIQIGNPASWAGALAARDESGGSIGAVTDPAIVEAYRLLAATVGVF
                     VEPASAAAVAGLLQAAAEGTVTSSDVVVCTLTGHGLKDPQRAIAEVQVGETVDADAAQ
                     VADAVGL"
     gene            544..1326
                     /locus_tag="VIJ44_01670"
     CDS             544..1326
                     /locus_tag="VIJ44_01670"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5164636.1"
                     /translation="MDIPVALVTGSSSGIGAAFARALGARGYQVILSARREDRLRGLA
                     AELERDHGRSAEVLVADLATDRGVQQVAARLGDTDRPIDLLVNNAGFGTAGALWELDA
                     EREEQEIRVNVVAVVRLTHAALSTMVGRGRGGVINVSSVAGYQPTPWGATYGATKAFV
                     SSFTHAVHEELRGTGVRVMVLAPGYTHTEFHEQAAMPVDQSPELLWQSPETVAEAALH
                     AYERGRVVCTPGPLNTVAAAFSATLPAAITRRVAGIVTRRTH"
     gene            1374..2891
                     /locus_tag="VIJ44_01675"
     CDS             1374..2891
                     /locus_tag="VIJ44_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEY5164637.1"
                     /translation="MPTLNAVVAARAARTPDGLAYIGDEHRVTWREYHERANRIAAQL
                     IEAGTQRGDRVAVLMPDGPSVHACYLGVERAGAVIVGIGPRAGRREVEHLLAVTDASV
                     LLAPPDLAVDAVPAGVPRLSITENEPPGDADASAVVGRRPIDADDLWLLNSTSGTTGL
                     PKCVMHHQRRWSAFHEYAVAAADLSPDDVFMSALPAPFGFGVWTAHVTPTLLGVPCVV
                     SARFDAEACLALIAREQVSVLAAVSTQFIMLLNSPELGRHDLSSLRVLFTGGEMVPEQ
                     RATEFEDRTGCTVLQFYGSNETGALSRTTLADTRARRLRTAGRVIPEMQVRLLADDGT
                     DVTATGGPGIAACRGALTCLGYYHDPDANAALYTADGWMRTGDRCTVDTDGYLTVRGR
                     ASDFIIRGGKNISAAQVEDEVATHPTVALTAAVAMPDPVFGERVCVYVELYPNSDLDL
                     DGLRDHLAARGVGKELWPEHLVVMDALPRSSGGKVAKGALRDDVRRRVGPGGTAP"
BASE COUNT          422 a         1084 c          991 g          411 t
ORIGIN      
        1 atccccgtcg gcaacgccgg caacatcacc gcgtactggc gcggatactg cgaggacgcc
       61 gaagcgggtc gagcgacccg ccggcctcgg atgctcggct ggcaggcaac cggagcggca
      121 ccgatcgtgc gcggtgaacc cgtgttgcat ccagagacgc tcgccacggc gatccagatc
      181 ggcaaccccg cctcctgggc gggggcgttg gcggcccgtg acgaatcagg cgggtccatc
      241 ggcgcggtca ccgacccggc gatcgtcgag gcctaccggc tcctcgccgc caccgtgggg
      301 gtattcgtcg agcccgcttc cgccgccgcc gtcgcggggc tcctgcaggc cgcggcggag
      361 ggaacggtca catcctccga cgtcgtcgtc tgcaccctca ccggacatgg actcaaggac
      421 ccccaacggg cgatcgccga ggtgcaggtc ggcgagaccg tcgacgccga cgccgcgcag
      481 gtcgccgacg ccgtcggcct ctgagcccga cggtcccggc ccgaccgtct ctaggctcgg
      541 ggtatggaca taccagtcgc gctcgtcacc gggtcgtcgt cgggcatcgg cgcggccttc
      601 gcccgggcgc tcggcgcccg gggctaccag gtgatcctca gcgcccgacg cgaggaccgg
      661 ctccgaggcc tcgccgccga gcttgagcgc gaccacgggc ggtcggccga ggtgctcgtc
      721 gccgacctcg cgaccgatcg gggcgtccag caggtcgcgg cccgtctcgg cgacacggac
      781 cggccgattg atctgctcgt caacaacgcc gggttcggga ccgccggggc cctctgggag
      841 cttgacgccg aacgcgagga acaagagatc cgcgtcaacg tcgtggccgt cgtccgcctg
      901 acacacgccg cgctttcgac gatggtcgga cgagggcgcg gcggcgtgat caacgtgtct
      961 tcggtcgccg ggtaccaacc caccccgtgg ggtgccacct atggggcgac gaaggcattc
     1021 gtgtcgagct tcacgcacgc cgtccacgag gagctccggg gaacgggtgt gcgggtgatg
     1081 gtcctcgccc cgggctacac ccacaccgag ttccatgagc aggcggccat gccggtcgac
     1141 cagtctcccg agctcctctg gcagtccccg gagacggtcg cggaggcggc gttgcacgcc
     1201 tacgagcgcg ggcgcgtcgt gtgcacgccg ggacccctca acaccgtggc cgccgcgttc
     1261 tccgcgacgt tgcccgccgc gatcactcgc cgggttgctg ggatcgtcac ccgccgcacg
     1321 cactgaccgt gcgcgcggaa tggcgcgccg agcagcggct accgtccgcg gccgtgccaa
     1381 cgctcaatgc cgtcgttgca gcccgtgccg cgcggacgcc ggacggcctg gcatacatcg
     1441 gtgacgaaca ccgcgtcaca tggcgtgagt accacgagcg cgccaaccgg atcgccgctc
     1501 aactcatcga agccggcacg cagcgcgggg accgggtcgc ggtactcatg cctgacggtc
     1561 cgtcggtgca cgcctgctac ctcggcgtcg aacgagccgg cgcggtcatc gtcggcatcg
     1621 gacctcgagc cggtcgacgc gaagtcgaac acctcctcgc ggtcacggac gcctcggtgt
     1681 tgctcgcccc gcccgacctc gcggtggatg ccgtgcccgc cggcgtgccc cgactctcca
     1741 tcaccgagaa cgagccacct ggagacgccg atgcgtccgc ggtggtcggg cgacgcccga
     1801 tcgacgctga cgacctgtgg ctcttgaact cgacgtcggg aacgaccggg ctgcccaagt
     1861 gtgtgatgca ccaccaacga cgctggtccg ccttccacga gtacgccgtc gctgccgccg
     1921 acctgtcgcc cgacgacgtc ttcatgagcg cactaccggc cccgttcggg ttcggggtct
     1981 ggaccgcgca cgtcacgccg accctcttgg gggtcccgtg cgtcgtgtcg gcacgcttcg
     2041 acgctgaggc gtgcctggcg ctcatcgcgc gcgaacaggt ctcggtcctc gccgcggtgt
     2101 cgacgcaatt catcatgctg ctcaactcgc ccgagctcgg ccgccacgac ttgtccagcc
     2161 tgcgggtcct cttcaccggc ggcgagatgg tccccgagca gcgcgccacg gaattcgagg
     2221 atcgcaccgg ctgcaccgtg ctccagttct acggttcgaa cgagacggga gcgttgagcc
     2281 gaaccacgct cgccgacacc cgggcccggc ggctccgaac cgcggggcgg gtgatccccg
     2341 agatgcaggt ccgcctcctc gcggacgacg gaaccgacgt caccgccacc ggcggacccg
     2401 gcatcgccgc gtgtcggggc gcgctcacct gcctcggcta ctaccacgac cccgacgcca
     2461 acgccgcgct ctacacggcc gacggctgga tgcggaccgg cgaccggtgc accgtggaca
     2521 ccgacggcta cctcaccgtg cggggccggg cctcggactt catcatccgg ggcgggaaga
     2581 acatcagcgc cgcccaagtc gaagacgagg tcgccaccca ccccacggtc gcgctcaccg
     2641 ccgccgtagc gatgccggat ccggtgttcg gtgagcgggt gtgcgtctac gtggagctgt
     2701 accccaactc agacctcgac ctcgacgggc tgcgcgatca cctcgcagcc cggggggtcg
     2761 gcaaggagct ctggcccgag cacctcgtgg tgatggacgc cttgccgcgc tcctcgggcg
     2821 gcaaggtcgc caaaggtgcg cttcgcgatg acgtccgacg ccgggtcggt cccggcggga
     2881 ccgcaccgtg accgcaccgc ggctcgag
//
LOCUS       DATCCH010000082         3486 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_175165, whole genome shotgun sequence.
ACCESSION   DATCCH010000082 DATCCH010000000
VERSION     DATCCH010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175165"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VIJ44_01680"
     CDS             <1..285
                     /locus_tag="VIJ44_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEY5164638.1"
                     /translation="DRFLQQEVWERMGVDVKAAVKVAMQDPDRPIFQSMLFSKIVPNC
                     KKLGLLDAADGWLRERFTELGVIQFEDWVDTGEEYATLDEVAKDRATATA"
     gene            376..2037
                     /locus_tag="VIJ44_01685"
     CDS             376..2037
                     /locus_tag="VIJ44_01685"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEY5164639.1"
                     /translation="MTAPARIVFLGGLGEIGRNCMCLEVDGRIIVIDCGLMFPEADMP
                     GIDLVLPDFTFLRDHADHVDGIVVTHGHEDHVGGLAYLLRDVSAPIYGSALSLGLARN
                     RIEEAGVLDRTELIAVRDGERRRIGPVDCEFIPVAHSVPHALAVAYFTPAGTIIHTGD
                     FKLDLTPVDGRRTDLARLGELGRGGVRLLLSDSTNAERAGFTASESTVGVVMRDLIRD
                     HPERRFIVASFASHLHRVQQVAREAVAAGRRVAFLGRSMTQNVSLAREMGLLDLPADR
                     VIDAEDVTRYAPGEVCIICTGSQGEPMSALALMASHEHKFIKVSSDDIVVLSAHAIPG
                     NEPNVHRVIDGLHRAGALVIHESTSPVHVSGHASREELKFVLGLVDPEWFVPVHGEYR
                     HLVHHAALAEDMGLEPDHVLICEDGDAVTIDDTGVDVERRAVPAGYQYVDGIGGDISH
                     GVLRDRRTLGEEGFVVVIVTVDAGTGEIVTGPEIVTRGWVYEAEADELLEDAKTAVRA
                     ALIHAAEDGPVEFEALRRHARRALGKLINERTRRRPAVIPVILEV"
     gene            2057..2845
                     /locus_tag="VIJ44_01690"
     CDS             2057..2845
                     /locus_tag="VIJ44_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003884178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5164640.1"
                     /translation="MTIRDWSNFIGGRTALITGSGAGVGRAIAIQMARAGADIWVNDL
                     DRDRAEGVCAEIEREGGTARPVLADVCDGDSVARMTDTTGPLDILVNNAGTGVRSWTE
                     SVRLVPFAETGPKDWDPVIRVNLAGVLHVTRSYLPAMLERRWGRVLVIVSDAGRRGER
                     KQVVYGAAKAAAMGFARGLAAEVGRDGITVNCISLGSMKHSTLADAIDADPDLEPKLA
                     RAYPVGRLGHVEDPAPLAVLLCSDAAEWITGQVYPVDGGYAPAL"
     gene            2934..>3486
                     /locus_tag="VIJ44_01695"
     CDS             2934..>3486
                     /locus_tag="VIJ44_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164641.1"
                     /translation="MASRTTTRSARRAPRPAKKPSGTRPSSSRRRPPPPEPPRHDFAA
                     AARRQLGAHRNDAIGVALLVLAALTALGLASDLAGTLGRGLADTLGALLGQARYGVPV
                     ACVVFAVLCLRARPHPPADPDPAEGPTDEEQPPGREPAPLRVGLGTVLLGLAVVGVLH
                     LTRGAPALDGPVAALRAAGGYLGA"
BASE COUNT          519 a         1426 c         1045 g          496 t
ORIGIN      
        1 gaccggttcc tccagcaaga ggtctgggag cggatgggcg tcgatgtcaa ggccgcggtc
       61 aaggttgcca tgcaagaccc cgaccggcca atcttccagt cgatgctgtt ctccaagatc
      121 gtcccgaact gcaagaagct cggtctcctc gacgccgccg acggctggct ccgggaacgg
      181 ttcaccgagc tcggcgtcat ccagttcgag gattgggtcg acactggcga ggagtacgcc
      241 acgctcgacg aggtcgccaa ggaccgcgcc actgccaccg cgtgaccgtc ccgcgtccac
      301 cacaaccgat ccgcgccgag cccccgacct cgtcgtggcc cggggggaac ccggcggggc
      361 agtaacttcg agcgcatgac cgcccccgcc cgaatcgtct tcctgggcgg cctcggcgag
      421 atcggccgca actgcatgtg cctcgaggtc gacgggcgca tcatcgtcat cgactgcggt
      481 ctcatgttcc ccgaggccga catgcccgga atcgaccttg tcctgcccga tttcaccttc
      541 ctgcgcgacc acgccgacca tgtcgacggc atcgtcgtga cccacggcca cgaagaccac
      601 gtcggcggcc tcgcctacct gctccgcgac gtctccgccc ccatctacgg ctccgcgctc
      661 tccctcggcc tcgcccggaa ccgcatcgaa gaggctggtg tcctcgaccg gaccgagctc
      721 atcgccgtcc gtgacggcga gcggcgacgc atcggccccg tcgactgcga gttcatcccc
      781 gtcgcccact ccgtcccgca cgccctcgcc gtcgcctact tcacccccgc cgggaccatc
      841 atccacaccg gcgacttcaa gctcgacctg acccccgtcg acgggcgacg caccgacctc
      901 gcccgcctcg gtgagctcgg ccgcggtggc gtccggcttc tgctctcgga ctccaccaac
      961 gccgaacggg ccgggttcac cgcctccgaa tccaccgtcg gggtcgtcat gcgcgacctc
     1021 atccgcgacc accccgaacg tcgcttcatc gtcgcttcgt tcgcgtcgca tctgcaccgc
     1081 gtccaacaag tcgcccgcga agcagtcgcc gccggccgcc gcgtcgcgtt cctcggacgg
     1141 tccatgaccc agaacgtcag cctcgcccgc gagatgggtc tcctcgacct gcccgccgat
     1201 cgagtcatcg acgccgaaga cgtcacccgc tacgcgccgg gcgaggtctg catcatctgc
     1261 accggctccc aaggcgaacc catgagcgcg ctcgccctca tggccagcca tgagcacaag
     1321 ttcatcaagg tcagcagcga cgacatcgtg gtcctctcgg cccatgccat tcccggcaac
     1381 gaacccaacg tgcatcgcgt catcgacggg ctccatcgcg ccggtgccct cgtcatccac
     1441 gagagcacct caccggtcca cgtctcgggt cacgcctccc gcgaggagct gaagttcgtc
     1501 ctcggcctcg tcgaccccga atggttcgtc cccgtccacg gcgagtaccg ccacctcgtc
     1561 caccacgccg cgctcgccga ggacatggga ctcgaacccg accacgtcct catctgcgaa
     1621 gacggcgacg ccgtcaccat cgacgacacc ggcgtcgacg tcgagcggcg cgccgtcccg
     1681 gccggctacc agtacgtcga cgggatcggc ggcgacatca gccacggcgt cctccgcgac
     1741 cgccgcaccc tcggcgaaga aggcttcgtc gtcgtcatcg tcaccgtcga cgccggcacc
     1801 ggcgagatcg tcaccggacc cgagatcgtc acccgaggct gggtctacga agccgaagcc
     1861 gacgagctcc tcgaagacgc caaaaccgcg gtgcgcgccg cgctcatcca cgccgccgag
     1921 gacggacccg tcgagttcga agcgctccgc cgccacgccc gccgggccct gggcaagctc
     1981 atcaacgaac ggacccgccg ccgccccgcc gtgatcccgg tcatcttgga ggtctgagca
     2041 tcgtgccgga gcgacggtga cgatccggga ctggtccaac ttcatcggcg gtcgcaccgc
     2101 gctcatcacg gggtccggcg ccggcgtcgg acgcgccatc gccatccaga tggcccgcgc
     2161 cggagccgac atctgggtca acgacctcga ccgtgaccgc gccgaaggcg tctgcgccga
     2221 gatcgaacgc gaaggcggca ccgcccgacc cgtcctcgcc gacgtctgcg acggcgactc
     2281 cgtcgcccgc atgaccgaca ccaccggacc gctcgacatc ctggtcaaca acgccggcac
     2341 cggcgtccga tcatggaccg agagcgtccg ccttgtcccg ttcgccgaga ccggaccgaa
     2401 ggactgggac cccgtcatcc gcgtgaacct ggccggtgtc ctccatgtca cccgctccta
     2461 ccttcccgcc atgctcgaac gacgatgggg ccgcgtgctc gtcatcgtct ccgacgccgg
     2521 ccgacgcggt gagcgcaaac aagtcgtcta cggcgccgcc aaagccgcgg ccatgggctt
     2581 cgcccgcggg ctcgcggccg aggtcggacg cgacggcatc accgtcaact gcatctccct
     2641 cggctccatg aagcactcca ccctcgccga cgccatcgac gccgaccccg acctcgagcc
     2701 caagctggcc cgcgcctacc cggtcggccg tctcggccac gtcgaagacc ccgcccccct
     2761 cgcggtgctg ctgtgcagcg acgccgccga atggatcacc ggccaggtgt accccgtcga
     2821 cggcgggtac gcccccgcgc tctaggggac ccgaggggcg atcgaccact gtcggtgccc
     2881 aaggggccca gtgtgtccgc tgacagattt gtggctggtg ttagcgtgac cgggtggcgt
     2941 cccgcaccac cacccgctcg gctcgccgcg ccccgcgccc ggcgaagaag ccaagtggaa
     3001 cgcgtccctc ctcgtcccgt cggcggccac cgccccccga gccgccccgc cacgacttcg
     3061 ccgccgcggc ccgccgtcag ctcggcgccc accgcaacga cgccatcggc gtcgccctcc
     3121 tcgtcctcgc cgcgctgacc gccctggggc tggcctcgga cctcgcgggg acgctcggcc
     3181 gcgggctcgc cgacaccctc ggggctctcc tcggccaggc ccgatacggc gtcccggtcg
     3241 cctgtgtggt cttcgcggtg ctgtgcctcc gagcccgacc tcatcccccc gccgaccccg
     3301 accccgccga gggtcccacc gacgaggagc agccgccggg ccgcgaaccg gcaccgcttc
     3361 gcgtcggact cggcaccgtg ctcctcgggc tcgccgtcgt cggcgtcctc catctcaccc
     3421 gcggcgcccc cgccctcgac ggaccggtcg cggcgctgcg tgccgccggc ggatacctcg
     3481 gcgccg
//
LOCUS       DATCCH010000083         6023 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_299694, whole genome shotgun sequence.
ACCESSION   DATCCH010000083 DATCCH010000000
VERSION     DATCCH010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6023
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299694"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="VIJ44_01700"
     CDS             complement(<1..433)
                     /locus_tag="VIJ44_01700"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY5164642.1"
                     /translation="MTKFFNLMSNGLALGAVYALVALGFVVIYRATRVINFAQGGLVV
                     FGAFLVYQAEHTWHWDFWIAAVFAMACCAAIGAAFQGVVLRRMIGQPVFAVILITIGL
                     FLLVEPITTTIWHNPPGGIETPWHLKTVSIGDVRILQVNIAT"
     gene            complement(446..2287)
                     /locus_tag="VIJ44_01705"
     CDS             complement(446..2287)
                     /locus_tag="VIJ44_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008357866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY5164643.1"
                     /translation="MTATVDVATVPPSGATPATLPAMLLAHAAARPRDAALRVKRLGR
                     WLELSWHDYATRAASMAWGLRGLGVEPGDRVAILSESRPEWLFADLAIQGIGAVSVGV
                     YTTSSEPEVAEVLRSSGAKIVIVEDEEQLDKTLAIRAELPQLAKIVVIDTRGIRSLGD
                     PMTISLDQLENLGEAPSRDIIDVWRDTVTGLAGGDDTAIVVYTSGVGGPPQGAMLSHR
                     NLIASAGVLASFYDARPDEEVLSYLPLCHVAERLVSVATAIHVGYVVNFGEGGESFVN
                     DLREVQPTFFLGVPRVWEKLMGSVQFRIGNASRLKRWMYSFWQRRGTRAADSRRRGRR
                     GPALPRGLAWFFLLHSLRHKLGLSRVRIALSGAAPIAPGVLEYWWSLGVPLRETYGQT
                     ENTALATANPATDVRVGTVGVALPGVTLRVADNGEILVRSPGNFVGYLGDPGGTRSAL
                     DGGGWLHTGDLGELDADGYLTITGRTKELIVTAGGLNVSPNRIENLIKVSPFVREAMV
                     IGDRRPYLTALIGVESAAVSDWARQQDLQFTNHRELVAQPEVRRLIERTIADVNRQVS
                     ETEQIRAFELLPFDLDDIDALTATQKVRRQQVAEECSDLIDRMYTAS"
     gene            complement(2313..3101)
                     /locus_tag="VIJ44_01710"
     CDS             complement(2313..3101)
                     /locus_tag="VIJ44_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008357867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5164644.1"
                     /translation="MSPESERGELLLDVQAITLRFGGVTALQGVSFQVHRNELFAIIG
                     PNGAGKTSIFNCLNAVYRPQTGHIGFDGDEIIGKRPSATAGLGIARTFQNLGLFVNLN
                     VIDNLMLGRHHLMKTGFVAGALWVGKAKREEVENRRRVEEIIELIELGPYRYQPVGLL
                     PYGIQKRIELGRALAMDPKLLLLDEPVAGMNLEETEDMARYLLQVKDELKLAMILVEH
                     DMHLVMDIADSVMALDFGVPIATGTPTQIQADPKVIEAYLGQPS"
     gene            complement(3098..3886)
                     /locus_tag="VIJ44_01715"
     CDS             complement(3098..3886)
                     /locus_tag="VIJ44_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017986100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5164645.1"
                     /translation="MLAVRDLEVVYGSAIHVLRGVSLDVEPGAIVALLGANGAGKTTL
                     LRAVSGLLRFHRGSVTQGSVAFEAERIDRLDAAAIVRKGISQVMEGRRIFAELTVDEN
                     LRAGAYSRRDRSAVKASYERVLELFPVIAERRRSTAGYLSGGEQQMLAMGRAMMASPR
                     LLVLDEPSLGLAPLIVQQIRDLIVEINHAGTSVLLVEQNATMALSIAQHGYVLENGLI
                     AKDGPSQALLADADIQEFYLGVGETAGRSFRDLKTYRRKKRWSA"
     gene            complement(4021..4497)
                     /locus_tag="VIJ44_01720"
     CDS             complement(4021..4497)
                     /locus_tag="VIJ44_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019884301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEY5164646.1"
                     /translation="MNTGDSTPRYVRPGWFTTHVFNPLVAALTRAGLSVWGSRELRVQ
                     GRTSGQWRRTPVNVLTVDDERYLVAPRGVTQWVRNLRVAGRGELRVGRRRESFQAVEL
                     SDVEKPAILRAYLKRWKAEVGVFFGGVSADSSDDELGRIAPDHPVFRITSGLERAA"
     gene            complement(4580..5233)
                     /locus_tag="VIJ44_01725"
     CDS             complement(4580..5233)
                     /locus_tag="VIJ44_01725"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HEY5164647.1"
                     /translation="MTTAAGPERDLGRASDAQYRFLDVIGGFNDSDLRRPSLLPGWTV
                     AHVLVHLARNADSHRRRAEAAARGDVIDQYPGGFEGRAAQIEGGAARTRAEILDDVGS
                     SADGMQTAWRRVPDSAWDVISRDVSGKERPLRSLPARRWQELEVHIVDVDAGVTHRGW
                     SADFVDVWLPALRATAEPRLPRGAALPAPGTLDDRDELAWLYGRFQRSDLPELTPWR"
     gene            complement(5284..>6023)
                     /locus_tag="VIJ44_01730"
     CDS             complement(5284..>6023)
                     /locus_tag="VIJ44_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164648.1"
                     /translation="RLVGMDLVRLALERADTATGAVDVIVGLLERYGQGGSGYDGTDD
                     PYWSSFLVADASDGWIVETSGTATATEHVTRTRAISNRLTIPAFDAAHRDPAMPTDFA
                     DPRLRSSEALLAAEPVSVAGVKDHLRDHTAGEHGFTVCMHVDDAVATTAALIAELPGP
                     FSTPRPLAHVCLGSPCRSIFVPVAVGRTLGRAPEWKQFIDVPAEACHQLGALEAELAA
                     SAPTNADSDAWAPEAWRRVEAVPAHPR"
BASE COUNT          952 a         1982 c         2157 g          932 t
ORIGIN      
        1 tcgtcgcgat gttgacctgg aggatcctga cgtccccgat cgagaccgtc ttgagatgcc
       61 agggcgtctc gatcccgccc ggcgggttgt gccagatggt ggtggtgatc ggctcgacga
      121 ggaggaagag cccgatcgtg atcaaaatga cggcgaagac gggctgaccg atcatccgtc
      181 gcagcacgac cccttggaag gccgcgccga tcgccgcgca gcaggccatg gcgaacaccg
      241 ccgcgatcca gaagtcccag tgccaggtgt gctcggcttg gtagacgagg aaggcgccga
      301 agacaaccag gccaccttgg gcgaagttga tgacccgcgt cgcgcggtag atgacgacga
      361 accccaacgc cacgagggcg tacacggctc cgagcgctaa cccgttgctc atcaggttga
      421 agaacttcgt cacggccgac tcctgtcagg aagcggtgta catccggtcg atcaggtccg
      481 agcactcctc ggcgacctgc tggcggcgaa ccttctgcgt cgcggtgagc gcatcgatgt
      541 cgtccagatc gaacgggagc agctcgaagg cccgaatctg ctcggtttcc gacacctgcc
      601 ggttcacgtc ggcaatggtg cgctcgatga gccgccgaac ctcgggttgg gcgacgagct
      661 cccgatggtt cgtgaactgc aggtcctgtt gacgagccca gtcggacacc gccgcggact
      721 ccacaccgat cagcgcggtc aagtaagggc gacggtcgcc gatgaccatc gcttcacgga
      781 cgaacggtga gaccttgatg aggttctcga tccggttcgg tgaaacgttc aggccaccgg
      841 cggtgacgat gagttccttg gtccggccgg tgatcgtcaa gtagccgtcg gcgtcgagct
      901 ccccgaggtc gccggtgtgg agccatccgc cgccgtcgag cgcggaccgg gtcccgccgg
      961 ggtcacccag gtagcccacg aagttgcccg ggctgcgcac gagaatctcg ccgttgtcgg
     1021 cgacccggag cgtcacgccg gggagtgcga cgccgaccgt gccaacccgc acatcggtcg
     1081 ccgggttcgc ggttgccaac gcggtgttct cggtctgccc gtacgtctct cgcagcggca
     1141 cgccgagtga ccaccagtac tcgagcaccc caggggcgat gggcgcggct ccggagagcg
     1201 cgatccgaac ccgggacagg ccgagcttgt gccgaaggct gtggagcagg aagaaccagg
     1261 ccagcccccg gggcaacgcc gggccccggc ggccgcggcg ccggctgtcg gcggcccggg
     1321 tacctcgccg ctgccagaac gagtacatcc accgcttgag gcggctggcg ttcccgatcc
     1381 ggaactggac cgatcccatc agcttctccc atacgcgggg gacgccgagg aagaacgtcg
     1441 gctgcacttc gcggaggtcg ttgacgaacg attcgccgcc ctcgccgaag ttgacgacgt
     1501 agccgacgtg gatcgccgtg gcgaccgaga ccagccgctc cgcgacgtgg cacaacggca
     1561 ggtaggacag gacctcctcg tcggggcggg cgtcgtagaa cgacgcgagg actccggctg
     1621 acgcgatgag gttgcggtgc gagagcatcg ccccctgggg tggtccgccg accccggagg
     1681 tatagacgac gatcgcggtg tcatcgcccc cggcgagtcc ggtgaccgtg tctcgccaga
     1741 cgtcgatgat gtctcgcgac ggggcctccc cgaggttctc gagctggtcg aggctgatcg
     1801 tcatcgggtc accgagggac cggatcccgc gggtgtcgat gaccacgatc ttggcgagct
     1861 ggggcagctc ggcacggatg gcgagtgtct tgtcgagctg ttcctcgtcc tcgacgatga
     1921 cgatcttggc gccggagcta cgcagcacct cggcgacctc aggttctgag ctggtggtgt
     1981 acacgccgac gctgaccgcg ccgatgccct ggatcgcgag atcggcgaag agccactcgg
     2041 gccggctctc gctcaagatg gcgacgcggt ccccgggctc gaccccgagg ccgcgcagac
     2101 cccacgccat gctggcggct cgcgtcgcgt agtcatgcca ggagagctcg agccaacggc
     2161 cgaggcgctt cacgcgcagg gccgcgtctc gcggtcgagc cgcggcgtgc gcgagcagca
     2221 tcgcgggcag cgtcgccggc gtcgcgccgg acggcgggac cgtggcgacg tcgaccgtcg
     2281 cggtcatggc gcgcacctcc cgtcgcgggc cctcacgatg gctgtccgag gtaggcctcg
     2341 atgaccttcg ggtcggcctg gatctgggtt ggggttccgg tggcgatcgg gacaccgaag
     2401 tcgagggcca tgaccgagtc ggcgatgtcc atgacgaggt gcatgtcgtg ctcgaccagg
     2461 atcatggcca gcttgagctc gtctttgacc tggagcagat accgggccat gtcttcggtt
     2521 tcctcaaggt tcatgcccgc gaccggttcg tcgaggagga gcaacttggg gtccatggcc
     2581 agtgcccggc ccagctcgat gcgcttctgg atcccgtacg ggagcaaccc gacgggctga
     2641 taccggtacg ggccgagctc gatgagctcg atgatctcct cgacgcggcg gcggttctcg
     2701 acttcctccc gtttggcttt cccgacccag agggcacctg cgacgaagcc ggtcttcatc
     2761 aggtggtgac ggcccagcat caggttgtcg atcacgttca ggttgacgaa cagcccgaga
     2821 ttctggaacg tccgggcgat tccgagcccc gcggtggccg acggccgctt cccgatgatc
     2881 tcgtcaccgt cgaagccgat gtggcccgtt tgcggccggt agacggcgtt caggcagttg
     2941 aagatggacg tcttgccggc gccgttgggt ccgatgatcg cgaacagttc gttgcggtga
     3001 acctggaacg acactccctg cagggcggtg acgcctccga agcgaagcgt gatcgcctgc
     3061 acgtcgagga gcagctcccc ccgttcggac tcgggactca tgcgctccac cgcttcttac
     3121 ggcggtaggt cttgaggtct cggaacgacc ggcccgcggt ctcgccgacc ccgaggtaga
     3181 actcttggat gtcggcgtcg gccagcaacg cctggctcgg gccgtccttg gcgatgaggc
     3241 cgttctcgag gacgtagccg tgctgggcga tcgagagggc catggtggcg ttctgttcga
     3301 cgagcagcac gctggtgccg gcgtggttga tctcgacgat gaggtcgcgg atctgctgca
     3361 cgatcagcgg agcgagaccg agcgaaggct cgtcgagcac gagcaggcgc ggtgaggcca
     3421 tcatcgctcg acccatcgcc agcatctgct gctcgccacc ggagaggtag ccggcggtcg
     3481 agcggcgtcg ttccgcgatg accgggaaca gctcgagcac ccgctcgtag gaggccttga
     3541 ccgcggatcg gtctcgacgg ctgtaagcac cggctcggag gttttcgtcc accgtgagct
     3601 cggcgaaaat tcgacggcct tccatgacct gggagatgcc tttacgaacg atggccgccg
     3661 cgtcgagccg gtcgatgcgc tctgcctcga acgcgacgct gccttgggtg acggatcccc
     3721 gatggaagcg aagcaatccg gaaacggctc gcagcagcgt cgtcttgccg gctccgttcg
     3781 cgccgagcag cgcgacgatc gcgccgggtt cgacatcgag gctcacaccc cgcagcacat
     3841 ggatggcgga cccgtagacg acctcgagat cccgtaccgc gaggatgggc gtgacgaggg
     3901 tcggcgtgct ttgtcccccc gacgccacgc ccgcacacta atgaaggagg tagaggtcct
     3961 caagggccgg ggcgccgacg gaccaatgga ccgtatgcgc cgcgggtctc ggctcgggat
     4021 tcaggcggcg cgctcgagcc ccgatgtgat gcggaacacc ggatggtcgg gagcgattcg
     4081 gccgagctcg tcgtcggatg agtcggcgct cactcccccg aagaagacgc cgacctcggc
     4141 cttccatcgc ttcaagtacg cgcgcaggat cgccggcttc tcgacgtcgg agagctcaac
     4201 ggcctgaaac gactctcggc ggcgcccgac gcgcagctcg ccgcgacccg cgacacgcag
     4261 gttcctgacc cactgggtca cgccgcgggg agcgacgagg taccgctcgt catcgacggt
     4321 caggacgttg acgggtgttc ggcgccattg gccgctggtg cggccctgga cccgcagctc
     4381 gcgcgaaccc cagacgctga gcccggcgcg ggtgagcgcg gcgacgaggg ggttgaagac
     4441 gtgggtcgtg aaccagcccg gacggacgta gcggggggtc gagtcaccag tgttcatggg
     4501 cacctccccg gcgggagacg ccgcggtggc gagcaaggtc ggcgacagcc cgagccgccg
     4561 cggacggcct tgggccggct caacgccacg gggtgagctc gggaaggtca ctgcgctgga
     4621 agcgtccata cagccaggcg agctcgtcgc gatcgtcgag cgtcccgggc gcgggaagcg
     4681 cagcaccgcg ggggaggcgg ggctcggcgg tggcgcgaag cgccgggagc cacacgtcga
     4741 cgaagtcggc ggaccagccg cgatgggtga cgccggcgtc gacgtcgacg atgtgcacct
     4801 cgagctcttg ccatcggcgg gccggcaggg accgcagcgg ccgctccttc ccagagacat
     4861 cacgcgagat gacgtcccac gcgctgtccg gcacccgccg ccaggcggtc tgcatcccgt
     4921 cggcgctgct gccgacgtca tcgaggatct cagcgcgggt ccgcgccgct ccgccttcga
     4981 tctgggcggc tcggccttcg aatccgcctg ggtactgatc gatcacgtcg ccgcgggccg
     5041 cggcttcggc gcggcgacgg tgactgtcgg cgttgcgcgc caggtggacc aggacatgcg
     5101 cgacggtcca gccgggcagg aggctcgggc gacgcagatc cgagtcgttg aagccgccga
     5161 tcacgtcgag gaaccggtac tgggcgtcgc tcgctcggcc gaggtcgcgc tcggggccgg
     5221 ctgctgtcgt cacggtggcc accctatgag cctgcaccgc gcccgacggt taggcgaccg
     5281 gcgtcaacgg ggatgggcgg gcacggcctc gacgcggcgc cacgcttcgg gagcccatgc
     5341 gtcggagtcg gcgttggtcg gcgccgaggc ggcgagctcg gcttcgagcg cgccgagctg
     5401 gtggcaggcc tcggcgggga cgtcgatgaa ctgcttccat tcgggggccc gcccgagggt
     5461 tcggcccacc gcaaccggga cgaagatcga acggcatggc gacccgaggc acacgtgcgc
     5521 cagcggccgt ggggtcgaga acgggcccgg gagctcggcg atcagcgccg cggtcgtcgc
     5581 caccgcgtcg tcgacgtgca tgcagaccgt gaacccgtgc tcaccggcgg tgtggtcgcg
     5641 cagatgatcc ttgacgccgg cgacgctgac cggctcggcg gcgagcagcg cctcactgga
     5701 gcgaagccgg gggtccgcga agtcggtcgg catcgccggg tcgcggtgcg cggcgtcgaa
     5761 cgctgggatc gtgagccgat tggagatcgc ccgggtccgc gtcacgtgct cggtcgcggt
     5821 cgcggtcccc gaggtctcga cgatccaacc gtccgaagcg tcggcgacca ggaacgacga
     5881 ccagtacggg tcatccgttc cgtcgtagcc gcttccgcct tgcccgtagc gttcgaggag
     5941 cccgacgatg acatcgacgg cgccggtggc ggtgtcagcc cgttcgagcg cgaggcggac
     6001 caggtccatg cccacgaggc gcg
//
LOCUS       DATCCH010000084         2133 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694 k141_4649,
            whole genome shotgun sequence.
ACCESSION   DATCCH010000084 DATCCH010000000
VERSION     DATCCH010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4649"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..1230)
                     /locus_tag="VIJ44_01735"
     CDS             complement(19..1230)
                     /locus_tag="VIJ44_01735"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005466189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEY5164649.1"
                     /translation="MPEAVIVAASRTPIGRANKGSLVDCRPDDLSAGVVRAVLDQVPA
                     LDPAEVEDLLWGCGQPAGESGYNVARVVAILAGLAQAPGATVNRYCSSSLQTIRMAGH
                     AIKAGEGDVFVAGGVETVSRFLKGMSDGLPGTHNPMFTDAEARSTAHGEGGDGPWKPN
                     DGFPDVYLAMGQTAENVAEQEHVSREEMDEFALRSQQRAVEAQKNGFFEREITPVTTP
                     DGTLVSTDDGPRPDTTLEKLSSLKPAFRPGGTITAGNACPLNDGAAATIVMSDTRAEA
                     LGITPKARIVASGVTAIDPEIMGLGPIEASRQALARAGMTIDQIDLVEINEAFAAQVI
                     PSARHLGIPLEKLNVHGGAIALGHPFGMTGARIMATLLNGLEDKGLRYGLETMCVGGG
                     QGMAMLVERLS"
     gene            1360..>2133
                     /locus_tag="VIJ44_01740"
     CDS             1360..>2133
                     /locus_tag="VIJ44_01740"
                     /inference="COORDINATES: protein motif:HMM:NF039283.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase-like domain-containing protein"
                     /protein_id="HEY5164650.1"
                     /translation="MVDESGYQPIENYGVIGDLHTVALVGVNGSIDFCSFPKFDSPTI
                     FAALLDKDRGGRFSIHPTLDDVSYKQLYLPDTNVLISRFLSDDGLSEVSDFMPIGIFP
                     HDHPRVLVRRVKCVRGVVRFDLVFDPRFDYARAEHRVETRDGEVIFASTGSDGTAVRL
                     RTPVPVTVGGDGVARATFELRAGEKAAFVMEEVADHLSSPSAAPNYVSDAFKDTVNFW
                     RTWIGRSQYAGRWRESVHRSALVLKLLVSQTHGSLVAAPT"
BASE COUNT          308 a          795 c          679 g          351 t
ORIGIN      
        1 gcgcgagcgc gctcagagct agctcagccg ctccacgagc atcgccatac cttgcccgcc
       61 gccgacgcac atcgtctcga gcccgtaccg cagacccttg tcctcgagcc cgttcagcag
      121 cgtggccatg atccgagccc cggtcatccc gaacgggtgc ccgagcgcga tcgcgccacc
      181 gtggacgttc agcttctcca acgggatccc gagatgtctc gccgacggga tcacctgcgc
      241 cgcgaacgcc tcgttgatct cgaccaggtc gatctggtcg atggtcatcc cggcgcgcgc
      301 tagcgcctgg cgactggcct cgatcgggcc gagccccatg atctcggggt cgatggcggt
      361 cacccccgac gccacgatgc gggccttcgg ggtgataccc aacgcttcgg cgcgggtgtc
      421 gctcatcacg atcgtcgccg cggccccgtc gttcagcgga caggcgttgc cggcggtgat
      481 ggtcccgccc ggacggaacg ccggtttcaa cgagctgagc ttctcgagcg tggtgtccgg
      541 gcgcggcccg tcgtcggtcg agaccagtgt cccgtccggc gtcgtcaccg gggtgatctc
      601 ccgttcgaag aacccgttct tctgggcttc caccgcccgt tgctgggacc gcaacgcgaa
      661 ctcgtccatc tcctctcggg acacgtgctc ctgctcggcg acgttctcgg ccgtctggcc
      721 catcgcgagg tacacgtcgg ggaagccgtc gttgggcttc cacggaccgt cgccgccttc
      781 gccgtgggcc gtgctgcgcg cctcggcgtc ggtgaacatc gggttgtggg tgccgggcag
      841 cccgtccgac atccccttga ggaaccggct gaccgtctcg acgccaccgg cgacgaacac
      901 gtcgccctca cccgccttga tggcgtgccc ggccatccgg atcgtctgca gcgacgagga
      961 gcagtaccgg ttcacggtcg ctcccggagc ctgagccagc ccggcgagga tggcgacgac
     1021 ccgggccacg ttgtaccccg actcgcccgc cggctggcca cagccccaga ggaggtcctc
     1081 gacctcggcc gggtccagcg ccgggacctg gtcgaggacg gcccgcacga cccccgccga
     1141 gaggtcatcg gggcgacagt cgacgaggga ccctttgttg gctcgtccga tcggggtccg
     1201 ggacgcggcg acgatcacgg cttcgggcat ggcgaggtct cctcagcgcc tgggatgctt
     1261 cccgcctgtc tacatccggc tcccactcgg gagcgaaaga cagcaggccc cccggcatcg
     1321 accggcggcc tgaacgcgag gaccagaggg gaaccactcg tggtcgacga gtccgggtac
     1381 cagccgatcg agaactacgg ggtcatcggc gacctgcaca ccgtcgcgct ggtcggggtg
     1441 aacgggtcca tcgacttctg ctcgttcccc aaattcgact caccgacgat cttcgccgcg
     1501 ctcctcgaca aggaccgcgg cggtcgcttc tccatccacc cgacgctcga cgacgtctcg
     1561 tacaagcagc tctacctgcc cgacaccaac gtgctcatca gccggttcct gtccgacgac
     1621 gggctctcgg aggtgtcgga cttcatgccg atcgggatct tcccgcacga ccatccccgg
     1681 gtcctcgtcc gacgggtgaa atgcgtacgc ggcgtggtcc gcttcgacct cgtcttcgat
     1741 cctcgcttcg attacgcccg ggccgagcat cgggtcgaga cccgcgacgg cgaggtgatc
     1801 ttcgcctcca ccggatccga cggcaccgcg gtgcggctcc gcaccccagt gcccgtcacc
     1861 gtcgggggcg acggtgtcgc ccgagccacc ttcgagctcc gggccgggga gaaggccgcc
     1921 ttcgtcatgg aggaggtcgc cgaccatctg agcagcccgt ccgcggcgcc gaactacgtc
     1981 tccgacgcct tcaaagacac cgtcaacttc tggcgcacct ggatcggccg ttcccagtac
     2041 gccggccgct ggcgcgagag cgtccaccgc tccgcactgg tcctcaagct gctcgtctcc
     2101 caaacccacg ggtcgctggt cgcggcgccc acc
//
LOCUS       DATCCH010000085         6104 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_92779, whole genome shotgun sequence.
ACCESSION   DATCCH010000085 DATCCH010000000
VERSION     DATCCH010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6104
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92779"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="VIJ44_01745"
     CDS             <1..855
                     /locus_tag="VIJ44_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010042158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY5164651.1"
                     /translation="RIIGQDEAVSAVANAIRRSRAGLSDPNRPIGSFLFLGPTGVGKT
                     ELARTLAEFLFDDERAMVRIDMSEYQEKHNVSRLVGAPPGYVGYDEGGQLTEAVRRRP
                     YAVVLLDEIEKAHPDVFNVLLQLLDDGRLTDGQGRTVDFTNTVLIMTSNLGGGADEAV
                     VMQAVQSSFKPEFLNRIDEIVMFHRLDERHIEQIVRLQVDQLRSRLAERNLGLELTEE
                     ALAHVAKIGYDPSFGARPLKRVLQKEVADPIALGLLKGDYHDGDVVQVDASSDAQLTF
                     SRSAAVTI"
     gene            1133..2425
                     /locus_tag="VIJ44_01750"
     CDS             1133..2425
                     /locus_tag="VIJ44_01750"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012157655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEY5164652.1"
                     /translation="MPGTVIVGTQWGDEGKGRFTDYLAKESSLVVRYQGGHNAGHTLV
                     VDGEVFALQLVPSGVLYDHVTPVIGNGVVIDPVVLVEELDALERKGIDTSRVRISGNA
                     HLIMPYHQELDRVTERYLGKNKLGTTKRGIGPAYADKALRVGLRVQDLLDAKIFRQKL
                     ELALREKNGVLAKVYNRLPLDADDICDRYLGLLPRLEPHIADTVHLVHEALEIGQHVL
                     FEGAQATYLDLDHGTYPFVTSSNPVAGGACIGAGVGPRAIERVIGVAKAYVTRVGSGP
                     FPSELPEGDPVGDFLVERGVEFGTNTGRRRRTGWLDTVMLRHAVWLNGCSELAVTKLD
                     VLAPLEELKVCVAYVGPDGTRYDHVPYHQSVLHKVRPVYETLPGWGTEIDGAARIEDL
                     PTAARDYLGFIADAAGVPVSFVSVGPSREQTVVLPRAA"
     gene            2422..3705
                     /gene="purD"
                     /locus_tag="VIJ44_01755"
     CDS             2422..3705
                     /gene="purD"
                     /locus_tag="VIJ44_01755"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013283401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEY5164653.1"
                     /translation="MSLRVLVVGGGGREHALAWALARSGSVSDVYALPGNPGAATVAQ
                     CVTGETDDPSAVAAVADDLAVDLVVVGPEPPLVAGVADAVAAHGHAVFGPSADAARLE
                     GSKAWMKALLAEVGVPTARHAVFGPGDDAAARSFLDTLDGLYVVKTDGLAAGKGVLVT
                     ESVTEARDAVGRYLSGEAFGDAGRTLVIEEGLRGPELSLLVVCNGDPDAAVPLAPAQD
                     FKRIGEHDTGPNTGGMGAYSPVPVVSTELIEQLMTTAVRPTLHALAARGAPYRGVLYA
                     GLMLTPDGPKVLEYNVRFGDPECQVVLPRLETDLGELCWRAASGSPLPPVRFHGDACV
                     GVVLASEGYPASPRTGDVIVGLEDAAALPDVAVFHAGTKRGDDGALATAGGRVLDVTA
                     LGPTIPAARRRAYEAAEKISWPGAQYRRDIAAAVA"
     gene            4064..5368
                     /gene="purB"
                     /locus_tag="VIJ44_01760"
     CDS             4064..5368
                     /gene="purB"
                     /locus_tag="VIJ44_01760"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEY5164654.1"
                     /translation="MIPRYALEPMASLFTDEARYAAWLEVEVLAVEAWAKLAVVPAED
                     AVAVRDRAGFDVAAIEAREQVTDHDVAAFVDVVQERVGGRAGSWIHYGLTSSDVVDTA
                     LSVTLVRAGDLLLEAVDALEAVVARRAREFRDTPIVGRTHGIHAEPTTFGAKLALWAL
                     ALRRDRRRLERARGGIAVGKLSGAVGTYSNVDPEVEAFVCERLGLRPVPATQVLGRDR
                     HAELLYACASLGATIETCALEIRHLQRTEVGEVAEPFRTGAQKGSSAMPHKRNPVKSE
                     QLCGLARVLRGYVVAGLEDVALWHERDISHSSVERIVLPDACQLAFYMAVRLRTVVDG
                     LEVYPDRMRENLHSSHGLVFSQAVLLALVEGGWTRDDAYRSVQRAAARSWADRRPFLD
                     VLLEDHDVVDVLGPARLEACFDLVGALAHAGRSVDALDESEF"
     gene            5368..>6104
                     /locus_tag="VIJ44_01765"
     CDS             5368..>6104
                     /locus_tag="VIJ44_01765"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF010568.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEY5164655.1"
                     /translation="MTDVALPHRYTGKVRDLYEVGPDRTLMVASDRISVFDVVLGDEI
                     PDKGRALTALSAFWFERTALVAPNHFLTADPAEFPDRAGPNVAGRAMLVRSAAPVRLE
                     CIARGYLFGSAWDEYEAVGTVCGRRLPPGLRRADRLPEPIFTTTTKVEPGAGHDEALT
                     DAVAAALVGDVVFERCRDSTLAVYRVGAARAEERGLILADTKLEFGLVGDEVLVIDEI
                     MTSDSSRYWDAAEYDVGISPPSFDKQFV"
BASE COUNT          915 a         2169 c         2080 g          940 t
ORIGIN      
        1 cgaatcatcg gtcaagacga ggcagtcagc gccgtcgcca acgccatccg ccgctcccgc
       61 gccgggcttt cggatcccaa ccgcccaatc ggatcgttcc tcttcctcgg tcccaccggc
      121 gtcggcaaga ccgagctcgc cagaaccctg gccgagttcc tgttcgacga cgagcgggcg
      181 atggttcgga tcgatatgtc ggaataccag gagaagcaca acgtgtcgcg cctggtcgga
      241 gcgcctccgg gctacgtcgg atacgacgaa ggtggccagc tcaccgaggc ggtccgacgc
      301 cgcccctacg cggtggtgct cctcgacgaa atcgaaaagg cccaccccga cgtcttcaac
      361 gtgttgctcc agctcctcga tgatgggcgg ctcaccgacg gccaaggccg gacggtggac
      421 ttcaccaaca ccgtcctgat catgacgagc aacctcggtg gcggcgccga cgaggccgtc
      481 gtcatgcagg cggtgcaaag cagcttcaag cccgagttct tgaaccgaat cgacgagatc
      541 gtcatgttcc atcgcctcga cgagcgccac atcgaacaga tcgtgcgcct ccaggttgac
      601 cagctccggt cgcggctggc ggaacggaac ctcggcctcg agctcaccga agaggccctg
      661 gcccacgtcg ccaagatcgg gtacgacccg tccttcggcg cccggccgtt gaagcgagtg
      721 ctccagaaag aggtggccga cccgatcgcg ctcggcctgc tcaagggcga ctaccacgac
      781 ggagacgtcg tccaggtcga cgcgagcagc gacgcccagc tcaccttcag tcggagcgcc
      841 gcggtcacca tctgaccacg gcgcctaccc ggccgcgagc acccccacac cctcggtccc
      901 ggcccgcggt tggaagcacc cggtcggcaa cgttgctgct tcgcgcatgc tcgtcggtca
      961 cgagcacatc ccggcacggc ccacccgaca acgaacctcg aggccgggca cgggtggggg
     1021 tcggcgcgaa ccgctcgcgg ctccgctccg ctccagcgaa agcgagcgac taccatgtcc
     1081 ctagcgtgac tgctgacgcg cgggaacgtt cgaagccgac ccggaggccg tcgtgcctgg
     1141 cacggtgatc gtcgggacgc aatggggtga cgagggcaaa ggtcgcttca ccgactacct
     1201 ggcgaaggaa agctcgctgg tggtccggta tcaaggcggc cacaacgccg gccacaccct
     1261 cgtcgtcgac ggcgaggtct tcgctctgca gctcgtcccg agcggggtgc tgtacgacca
     1321 cgtcacgccg gtcatcggca acggcgtcgt gatcgacccg gtcgtgctcg tcgaggagct
     1381 cgacgccctc gagcgcaagg ggatcgatac cagccgggtg cggatctccg gcaacgccca
     1441 cttgatcatg ccgtaccacc aagagctcga ccgggtcacc gagcggtacc tcgggaagaa
     1501 caagctcggc acgacgaagc gagggatcgg gccggcctac gccgacaagg cgctccgcgt
     1561 cgggttgcgc gtccaagacc tcctcgacgc caagattttc cgccagaagc tcgagctcgc
     1621 cctccgcgag aagaacggcg tcctggccaa ggtctacaac cggcttccgc tcgacgccga
     1681 tgacatctgc gaccgttatc tcggactcct ccctcgactc gagccgcaca tcgccgacac
     1741 cgtccacctg gtgcacgagg ccttggagat cggtcaacac gtccttttcg agggggcgca
     1801 agccacctac ctcgacctcg accacggcac ctatccgttc gtgacgtcgt cgaatccggt
     1861 cgccggcggc gcgtgcatcg gcgccggtgt cggccctcgg gccatcgagc gggtcatcgg
     1921 ggtcgcgaag gcgtacgtca cacgcgtcgg gagcggaccc ttcccctccg agctgccgga
     1981 gggcgacccc gtcggcgact ttctcgtcga gcgcggggtc gagttcggca ccaacaccgg
     2041 tcggcgccgc cggaccggct ggctcgacac ggtgatgctg cgccacgccg tgtggctcaa
     2101 cggctgcagc gagttggccg tcaccaagct cgacgtcttg gcgccgctcg aggagctcaa
     2161 ggtctgcgtc gcctacgtcg ggcccgacgg aacgcggtac gaccatgtcc cgtaccacca
     2221 atcggtgctg cacaaggttc gcccggtcta cgagacgctg ccgggatggg gaaccgagat
     2281 cgacggtgcc gcccgcatcg aggacctacc caccgccgca cgcgactacc tgggcttcat
     2341 cgccgacgcg gcgggtgtgc ccgtgagctt cgtctccgtc gggcccagtc gcgagcagac
     2401 ggtcgtcctt ccccgcgccg cgtgagcttg cgggtcctcg tcgtcggcgg cggcgggcgg
     2461 gagcacgcgc ttgcgtgggc gctcgcccgc tcggggtcgg tcagtgacgt ctacgcgctc
     2521 cccggcaatc ccggcgccgc gaccgtggcc caatgcgtga ctggagagac cgacgacccg
     2581 tccgcggtcg ccgcggtcgc cgacgacctc gcggtcgacc tggtggtcgt cgggcccgaa
     2641 ccgcccctcg tcgcgggcgt cgccgacgcg gtcgccgctc acggccacgc ggtcttcggg
     2701 ccgagcgccg acgcggcgcg cctggagggc tcgaaggcgt ggatgaaggc gctgctggcg
     2761 gaggtgggtg tgccgacggc gcggcacgcg gtgttcgggc ccggcgatga cgccgcggcc
     2821 cggtccttcc tcgacaccct ggacgggctc tacgtggtca agaccgatgg gctcgcggcc
     2881 gggaaagggg tgctcgtcac cgaatcggtg accgaagccc gagatgccgt tggccgatac
     2941 ctcagcgggg aggcgttcgg agatgccggg cgcaccctgg tgatcgagga aggcctgcgc
     3001 ggtcctgagc tgtcgctgct cgtcgtgtgc aacggtgacc cggacgcggc ggttcccctc
     3061 gcaccggccc aggacttcaa acggatcggc gaacacgaca ccgggccgaa caccggcggg
     3121 atgggtgcct actcaccggt gccggtggtg agcaccgagc tgatcgaaca gctcatgacc
     3181 accgcggttc gccccaccct gcacgccctc gccgctcgcg gcgcgcccta ccgcggagtc
     3241 ctctacgccg ggctgatgct cacccccgac ggaccgaagg tgctcgagta caacgtgcgc
     3301 ttcggcgacc cggaatgcca agttgtgctc cctcgactcg aaaccgatct gggcgagctg
     3361 tgctggcgcg ccgcgtcagg ctcgccgctt ccgccggtgc gcttccacgg cgacgcctgc
     3421 gtcggggtcg tcctcgcgag tgagggctat cccgcgtcgc cccgcaccgg ggatgtcatc
     3481 gtcggcctcg aggacgccgc cgcgctgcct gacgtcgcgg tcttccacgc cggaacgaaa
     3541 cggggcgacg acggcgcgct cgccaccgcg ggcggtcggg tcctcgacgt caccgcgctc
     3601 ggtccgacga tcccggccgc tcgacgccgg gcctatgagg cagcggagaa gatcagctgg
     3661 cccggcgccc aataccggcg agacatcgcc gcggccgtcg catgacctgg ctagtggatc
     3721 ggccgccggc gggtccgact gatgtccatc ggcgccgccc gacgagcgcc gcggacccga
     3781 atccccgccg cgacgagtgc gcaatcgatg cagcgcgtcg gctgtcgctt gcgggtgggc
     3841 ggcaccaggc aatccgcaca ccacaacttg gcgcagtcct tgcagcggcc ggcggcacgc
     3901 tcactccagt gcttcgcgca cgtgccgacc agaccgccgg tcggctcact gctcctcacg
     3961 ggcgcccctc ccggtgtctc acggttcgtt cgtggtcctc ggggcgtact cggtcgcgct
     4021 ccgggatccg gcccctagag tgtcgaccag gaggcagcgc gccttgatcc cccgctacgc
     4081 cctcgagccg atggccagcc tcttcaccga cgaggcgagg tatgcggcct ggctcgaggt
     4141 ggaggtcctg gccgtcgagg cgtgggcgaa gctggccgtc gtccccgccg aggacgccgt
     4201 cgccgtccgc gaccgggccg ggttcgacgt cgccgccatc gaggcgcgcg agcaggtcac
     4261 cgaccatgac gtcgccgcgt tcgtcgatgt cgtccaggag cgagtcggcg gtcgcgccgg
     4321 atcgtggatc cactacgggc tcacgtcgag cgacgtcgtc gacacggcct tgtcggtgac
     4381 cctggtgcgg gccggtgacc tccttctcga ggcggtcgac gcgctggagg cagtcgtcgc
     4441 ccggcgagcc cgcgagtttc gtgacacccc gatcgtcggg cggacccacg gcatccacgc
     4501 cgagccgacc acgttcggag cgaaactggc gttgtgggcg ctcgcgctgc ggcgggaccg
     4561 tcgccgcctc gagcgagccc gcggcgggat cgcggtgggg aagctctcgg gtgcggtcgg
     4621 gacctactcg aatgtcgatc ccgaggtcga ggcattcgtg tgcgaacggc tcgggttgcg
     4681 tccggtgccg gccacccagg tcctcggccg tgaccgacac gcggaactgt tgtacgcctg
     4741 cgcgtcgctc ggggccacga tcgagacgtg cgcgctcgag atccgacacc tccaacgaac
     4801 cgaggtgggg gaagtcgcgg agccgttccg aaccggagct cagaagggct cgagcgcgat
     4861 gccccacaag cgcaacccgg tcaagagcga gcagctgtgc gggctggctc gggtgctccg
     4921 cgggtacgtc gtcgcgggac tcgaagacgt agcgctctgg cacgaacgcg acatctccca
     4981 ctcgtcggtg gagcggatcg tgctccccga cgcctgccag ctcgcgttct acatggcggt
     5041 ccgcctgcgg accgtggtcg atgggctcga ggtctacccc gaccgcatgc gcgagaacct
     5101 gcactcctcg cacggactgg tgttcagcca agcggtgctc ctcgccctcg tcgagggcgg
     5161 ttggacgcgc gacgacgcgt accggtcggt gcaacgtgcc gcggcgcgct catgggcgga
     5221 tcgtcgaccg ttccttgatg tcctcctcga ggatcacgat gtcgtcgacg tgctcggccc
     5281 ggctcggctc gaggcctgct tcgacctggt cggcgcgctc gctcacgccg ggcggagcgt
     5341 cgacgcgctc gacgagtcgg agttctgatg accgacgtcg cgttgcctca ccggtacacc
     5401 ggtaaggtcc gtgacctgta cgaagtcgga cccgaccgca ccctcatggt cgcgtccgat
     5461 cgcatctcgg tgttcgacgt cgtactcggc gacgagatcc ccgacaaggg ccgagccctc
     5521 accgcgctgt cggcgttctg gttcgagcgg accgctttgg tcgcgccgaa ccatttcctc
     5581 accgccgacc ctgcggaatt cccggaccgt gccggcccga acgtggcggg gcgcgccatg
     5641 ctcgtccgct ccgcggcgcc ggtgcggctt gagtgcatcg ctcggggcta cctgttcggg
     5701 tccgcgtggg atgagtatga agccgtgggc acagtctgtg gccgaaggct tccgcccggg
     5761 ctccgacgcg ccgaccggct cccggagccc atcttcacca cgactacgaa ggtcgagccc
     5821 ggcgccggcc acgacgaggc tctcaccgac gcggtggcgg cggccctcgt gggcgacgtg
     5881 gtcttcgaac ggtgccgcga ctcgacgttg gcggtgtacc gcgtcggggc ggctcgggcc
     5941 gaggaacgag gtctgatcct cgccgacacg aagctcgagt tcggtctggt cggcgacgag
     6001 gttctcgtga tcgacgaaat catgacgtcg gactcgtccc gctattggga cgccgccgag
     6061 tacgacgtcg ggatctcgcc accgtcgttc gacaagcagt tcgt
//
LOCUS       DATCCH010000086         2670 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_258452, whole genome shotgun sequence.
ACCESSION   DATCCH010000086 DATCCH010000000
VERSION     DATCCH010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258452"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1608)
                     /locus_tag="VIJ44_01770"
     CDS             complement(<1..1608)
                     /locus_tag="VIJ44_01770"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEY5164656.1"
                     /translation="MTDRAIIGRTISESVPVFDASPRPPADAPNIVLVVLDDLGFGQL
                     GCFGSDLATPTIDRLAAEGARYNRFHVTALCSPTRACLLTGRNHHAVGMGFLTDVPTG
                     FPGYNGRIPPSAAALPRILRDGGYSTFAVGKWHLAPRWEQSASGPFARWPLGLGFERF
                     YGFLGGDTNQWTPELVRDNGFIEPPRSPMDGYHLTEDLVDQAMRLVQDQQQATPKKPF
                     FLYFATGATHAPHHAPRDAIDRYRGHFDAGWDAWRAATFDRQLASGVVPAGTTLTPRP
                     PWVDAWDNLSGDERRLFARLMEAFAGFLSHADEQLGRLVAHLETLGVLDDTLVLLCSD
                     NGTSAEGGPYGSLNEHRFTHQLVDDPTETLTHLDELGGFRAYNHYPWGWAWAGNTPLR
                     LWKRYTWLGGVRTPLVARWPSRITEPSGIRHQFCHAVDIFPTILDAAGIEVPTSVDGV
                     IQQPVDGASLLPTLADQEAPSPRATQYFEMIGSRAIYHDGWKATTDHIGNQLDIERAR
                     LEGSRDYDTDHWALFDLDEDFSEAHDRS"
     gene            complement(1664..2662)
                     /locus_tag="VIJ44_01775"
     CDS             complement(1664..2662)
                     /locus_tag="VIJ44_01775"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEY5164657.1"
                     /translation="MTPARAVGAPVPSLRFGDGARNGGDGTGQCHLRSTSHLVTSEGP
                     AMAKISDILNEGRTISFEFFPPRTAEAEAQLEQTLRDLEPLRPSYVSVTYGAGGATRE
                     RTHELVVQINRDLDLTAMAHLTCAAHSRAELEEILANYRDARIENVLALGGDPPSDVD
                     LPPTELDHAVDLVRLARGIGDFSIGVAVHPEPHPRSPTLESDRRFTAEKLAEADFGIT
                     QFFFEPEHYFELVDSLHRHDVDKPVIPGIMPVLSLAGIQRMAQLQGSALPAWLVERLH
                     TVEDDPAAVRRVGIDEATRLCSTLLEGGAPGLHFYTLNRSPATREIAANLGLAPTH"
BASE COUNT          399 a          810 c         1038 g          423 t
ORIGIN      
        1 ggagcggtcg tgggcctcgg agaagtcctc gtcgaggtcg aagagggccc agtggtcggt
       61 gtcatagtcg cggctgcctt cgagccgagc gcgttcgatg tcgagctggt tgccgatgtg
      121 gtcggtggtc gccttccagc cgtcgtggta gatggcacga gagccgatca tctcgaagta
      181 ctgcgtggcg cgggggctgg gtgcttcctg gtcggcgagg gttggcagga ggctcgcccc
      241 gtcgactggt tgctggatga caccgtcgac gctggtgggg acctcgatcc ccgcggcgtc
      301 gaggatcgtg gggaagatgt cgacggcgtg gcagaactgg tggcggatgc cgctcggctc
      361 ggtgatccgc gagggccacc gagcgacgag cggggtgcgg acgccgccca gccaggtgta
      421 gcgcttccac agccgcaggg gcgtgttgcc ggcccaggcc cagccccatg gatagtggtt
      481 gtaggcccgg aatccgccga gctcgtcgag gtgggtgagc gtctcggtgg ggtcgtcaac
      541 gagctggtga gtgaagcgat gctcgttgag cgacccgtac gggccgcctt cggcgctggt
      601 gccgttgtcg gagcacagca gcacgagggt gtcgtcgagg acgccgaggg tctcgaggtg
      661 ggcgacgaga cgcccgagct gctcgtcggc gtggctgagg aacccggcga aggcctccat
      721 gagccgggcg aagagacgcc gctcgtcgcc cgagaggttg tcccaagcgt cgacccacgg
      781 cggccgcggg gtcagggtcg tcccggcggg caccactccc gaggcgagtt gacggtcgaa
      841 ggttgcggcc cgccacgcgt cccagccggc gtcgaagtgc ccgcggtagc ggtcgatggc
      901 gtcgcggggc gcgtggtgcg gcgcgtgggt cgcgccggtg gcgaagtaga ggaagaacgg
      961 tttcttgggt gtggcctgct gttggtcctg gaccaggcgc atggcctggt cgacgaggtc
     1021 ctcggtgagg tggtagccgt ccatcggtga gcgcggcggc tcgatgaacc cgttgtcgcg
     1081 gacaagctcg ggcgtccact ggttggtgtc ccctccaagg aagccgtaga agcgctcgaa
     1141 acccaagccg agaggccaac gagcgaacgg ccccgacgcg ctctgctccc agcggggcgc
     1201 gaggtgccat ttgcccaccg cgaaggtgct gtacccgccg tcgcggagga tccgcggcaa
     1261 cgcggcggcc gacggtggga tccgcccgtt gtaccccggg aacccggtcg gcacgtcggt
     1321 gaggaacccc atgccgaccg cgtggtggtt ccggccggtg aggagacagg cccgggtcgg
     1381 cgagcacaac gcggtgacat ggaagcggtt gtaccgggct ccttccgcgg ccagtcgatc
     1441 gatggttggc gttgcgagat cggagccgaa acagccgagc tggccgaaac cgaggtcgtc
     1501 gagcacgacg aggacgatgt tgggagcatc cgccggcggt cgcggcgacg cgtcgaacac
     1561 cggcaccgat tcggagatcg tgcgtccgat gatcgcccgg tcggtcacgt tgccctcctc
     1621 agcgccgtcg acccacgcga cgacggtcgg cgttgacggt cgatcagtgg gtcggcgcca
     1681 aaccgaggtt ggccgcgatc tcgcgggtcg cgggcgaacg gttgagcgtg tagaagtgca
     1741 aacccggtgc gccgccttcg aggagggttg agcacagccg ggtggcttcg tcgatcccga
     1801 cccgtcgcac cgcggcggga tcgtcctcga ccgtatggag tcgctcgacc agccacgccg
     1861 ggagcgccga gccttgcagc tgggccatgc gctggatccc agcgaggctg agcaccggca
     1921 tgatcccggg gatcaccggc ttgtcgacgt cgtgtcggtg cagcgaatcg acgagctcga
     1981 agtagtgctc gggctcgaag aagaactggg tgatcccgaa gtccgcctcg gcgagcttct
     2041 cggcggtgaa gcgccggtca ctctcgagcg tcggagagcg agggtgcggc tcggggtgca
     2101 ccgcgacgcc gatcgagaag tcgccgatgc cccgcgccag tcggacgagg tcgacggcgt
     2161 ggtcgagttc ggtgggcggc aggtcgacgt cgctcggcgg gtcgcctccg agggccagga
     2221 cgttctcgat gcgggcatcc cggtaattgg cgagaatctc ttcgagctcg gcccggctgt
     2281 gcgcggcgca ggtcaggtgg gccatggccg tcaggtccag gtcacggttg atctggacga
     2341 cgagctcgtg ggtccgttca cgggtggcac cgccggcgcc atacgtgacc gacacgtacg
     2401 aggggcggag cggctccagg tcacggaggg tctgctcgag ctgcgcctcg gcttcggcgg
     2461 tccggggcgg gaagaactcg aacgagatgg tgcggccctc gttcaggatg tcgctgatct
     2521 tggccatggc gggaccctcg gaggtgacga gatgcgaggt cgagcgtagg tgacactgcc
     2581 cggtcccgtc accgccgttt cgtgccccat cgccgaatcg cagcgagggg acgggtgccc
     2641 cgaccgcgcg cgcgggagtc atgacagact
//
LOCUS       DATCCH010000087         2642 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694 k141_4855,
            whole genome shotgun sequence.
ACCESSION   DATCCH010000087 DATCCH010000000
VERSION     DATCCH010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4855"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1404
                     /locus_tag="VIJ44_01780"
     CDS             <1..1404
                     /locus_tag="VIJ44_01780"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY5164658.1"
                     /translation="VLADEAGAALDGPAIEAAVASLAGGLRAAGVRHGDVVAWQAPNW
                     HEVILLYRACWRLGAIAGPIHHHAGPADVDQFLDVLRPRLWLPPDAIRGGAARYPALL
                     GGEPVTSSAARPSDLAVVLFTSGSTGGPKAALHTQRGLAYKARVMADAHQLTPADAIL
                     MPAPLAHVSGLLNAVLVPGVVPMRVNLMARWEPEAALATIDTEAITFMIGPPTFFISL
                     MESPRFRAPRVASLRLVSSGGAGVTPTFVSDASERLHATVKRTYGSTEAPTVTTSTPS
                     DSPGRARDTDGRAVGSVRLRVSDPVTGQPRPHGETGELWLRGPELFAGYVDASQTEAA
                     ISRGWFRTGDLATVDADGWLTVVGRIKDTIIRGGENIAAAEVEAILEQHPDVVQAVAV
                     GYSDARYGERVCAFVVARRPFDLGICRAWFAHEGVARFKTPERVVMLDELPLLSAGKA
                     DRAALRARAEADAPDPG"
     gene            complement(1426..2514)
                     /locus_tag="VIJ44_01785"
     CDS             complement(1426..2514)
                     /locus_tag="VIJ44_01785"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY5164659.1"
                     /translation="MDFALNDDQEALRDGARDVLDGLASSAQVRAVVDAGGGWDSVLW
                     GAMVEQGWPGVAVPDSLGGLGLGTVEVAVLLEEVGRHVAPAPFASTVLALDALTGAGH
                     TGRVEDLLRGDVLAGVAWSRRGDAVRAETDGSHWRLHGRPDPVLYAPSALVAIVVAEG
                     GDGPGLFRVDLDPEHPARAEPAMDRTRELGWLRFNGTPATRLGGADSVERLINRGATY
                     AAAEMLGGADRALTMAVEYAKERVQFGRPIGSFQAVKHRCADMLVDVEGMRSSVYWAA
                     WCISADHPDASVAASTAKIWCSDASKRVMASALQVHGGIGFTWEHDLHLFMKRAQLDQ
                     LSFGDASYHRERLAALLRPRVEAGESIL"
BASE COUNT          394 a          984 c          899 g          365 t
ORIGIN      
        1 gtgctggccg acgaggccgg cgccgcgctc gacggccccg ccatcgaggc cgcggtcgcg
       61 tcgttggccg gcgggctccg cgccgccggt gtgcgccacg gcgatgtcgt ggcgtggcaa
      121 gcgccgaact ggcacgaggt gatcctcttg taccgggcct gctggcgatt gggcgcaatc
      181 gcgggtccga ttcaccatca cgcggggcca gctgacgtcg accagttcct cgatgtcctg
      241 cgaccaaggc tgtggcttcc acccgacgcc atccgtggag gcgccgcccg ctatcccgcc
      301 ctgctcggcg gcgagcccgt cacgtcctcc gcggcgcggc cgagcgatct ggcggtggtc
      361 ctgttcacgt ccggatcgac gggagggccg aaggccgcgc tgcacaccca gcggggcctc
      421 gcctacaagg cgcgcgtcat ggccgatgcg catcaactga cacccgccga cgcgatcttg
      481 atgccggcgc ccctcgctca cgtctcgggt ctgctcaacg cggtgctggt gcccggggtg
      541 gtgccgatgc gggtgaacct catggcccgg tgggagcccg aagctgcgct ggcgacgatc
      601 gacactgagg cgatcacctt catgatcggc ccaccgacgt tcttcatctc gctcatggag
      661 tcgccacggt tccgggcccc gcgagtcgcg agcctgcgct tggtgtcgag cggaggcgcc
      721 ggcgtgacgc caacgttcgt gtccgacgcc agcgaacggc tccacgcgac ggtcaaacgc
      781 acttacggct cgaccgaagc cccgacggtg acgacgagca cccccagcga ctcgcctggt
      841 cgcgcccgcg acaccgacgg ccgggctgtc ggttcggtgc gcctgcgggt cagcgacccc
      901 gtcaccgggc agccccgtcc ccacggcgag acgggtgaac tgtggctgcg aggacccgag
      961 ctcttcgccg gctacgtcga cgcgtcccag accgaggcgg ccatcagccg gggatggttc
     1021 cgaaccggtg acctcgccac cgtggatgcc gacgggtggc tgaccgtcgt cgggcggatc
     1081 aaggacacga tcatccgcgg tggggagaac atcgccgccg ccgaggtcga ggccatcctc
     1141 gagcagcacc ccgacgtggt acaggccgtc gcggtcggct actccgatgc ccgctacggg
     1201 gagcgggtct gcgccttcgt cgtcgctcgt cgacccttcg acctcgggat ctgccgggcc
     1261 tggttcgccc acgagggcgt ggcacggttc aagacgccag agcgggtggt catgctcgac
     1321 gagctgccgc tgctctccgc cgggaaggcc gaccgggccg cgcttcgcgc gcgcgccgag
     1381 gcggacgcac cagacccggg ttgaccgggc accggcggtt ccgagctaca ggatgctctc
     1441 cccggcctcg acgcggggcc gcagcaacgc cgccaaccgc tcgcggtgat acgacgcgtc
     1501 accgaacgac aactggtcga gctgggcccg cttcatgaac agatgcaggt cgtgctccca
     1561 ggtgaagccg ataccgccgt gcacctgcag cgcagacgcc atcacacgct tcgacgcgtc
     1621 ggaacaccat atctttgcgg tggacgcggc gaccgacgca tccggatgat cggcactgat
     1681 gcaccacgcc gcccaataga cgctggaacg catcccttcg acgtcgacga gcatgtcggc
     1741 acaccgatgc ttgacagcct ggaagctgcc gatgggtcgg ccgaactgaa cccgctcttt
     1801 ggcgtactcg accgccatcg tcaaggctcg gtcggcgccg ccgagcatct ccgcagcggc
     1861 atacgtcgcg ccccggttga tcagcctctc caccgagtcc gcgcctccga ggcgggtcgc
     1921 cggcgtgccg ttgaagcgaa gccagccgag ctctcgggtc cgatccatcg ccggctccgc
     1981 gcgggccgga tgctcagggt cgaggtccac gcggaacaac ccgggcccgt cgccgccttc
     2041 ggccaccacg atcgcgacga gggcggaggg cgcgtagagg accgggtcgg gtcgcccgtg
     2101 cagccgccag tgggacccgt ctgtctcggc gcgtacggcg tcgcctcgcc ggctccacgc
     2161 cacgccggcg agcacgtcgc cccggagcag atcctcgact cggccggtgt ggccggcccc
     2221 ggtgagagcg tccagcgcga gcaccgtcga cgcgaagggt gccggcgcca cgtggcgccc
     2281 cacttcctcc aggaggaccg cgacctcgac cgtgccgagg cccagcccgc ccagcgagtc
     2341 gggaaccgcg acgccgggcc aaccctgctc gaccatggcc ccccacagga ccgagtccca
     2401 ccccccgccg gcgtccacga cggcgcgaac ctgcgcactc gacgcgaggc catcgagcac
     2461 gtcgcgggcg ccgtcgcgca gcgcctcctg gtcgtcattc aacgcgaagt ccaagcggcg
     2521 gcaggctatc cctgcccttc gagcgtcgcc ggacgaccgc tacgctgagc gcaaccccga
     2581 gacaggagcc gtgcggtgac cgacgaaaag cccgcgtacc ttcgtcacgg cgacaccgag
     2641 gt
//
LOCUS       DATCCH010000088         2275 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_507765, whole genome shotgun sequence.
ACCESSION   DATCCH010000088 DATCCH010000000
VERSION     DATCCH010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507765"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..121)
                     /locus_tag="VIJ44_01790"
     CDS             complement(<1..121)
                     /locus_tag="VIJ44_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009076481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HEY5164660.1"
                     /translation="MHVGIMTREFPPDVYGGAGVHVDFLVRELRRLADVDVSCF"
     gene            144..1367
                     /gene="glgC"
                     /locus_tag="VIJ44_01795"
     CDS             144..1367
                     /gene="glgC"
                     /locus_tag="VIJ44_01795"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018684039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEY5164661.1"
                     /translation="MASRRRVLGIVLAGGAGKRLMPLTADRAKPAVPFGGQYRLVDFV
                     LSNLVNAGYLRLCVLTQYKSHSLDRHITTTWRMSTLLGNYVTPVPAQQRLGPRWYTGS
                     ADAIFQSLNLVYDDQPDYVVVFGADHVYRMDPSQMVQDHIDSGAGVTVAGIRVPKAQA
                     SGFGIIETEANGKDIKAFLEKPADPPTVPGHPDLCYASMGNYVFSTDVLIEALKVDSS
                     DEGSVHDMGGDIIPMMTRLGAAKVYDFADNDVPGATDRDRGYWRDVGTLDSYHDAHMD
                     LVSVHPIFNLYNRQWPILSHMGAWPPAKFVEGGEAHESIVGAGTIISGGLVDHSVVAF
                     DVRVGHGAEITDSVLLPGCTVGRGAVVRRAILDKGVVIPDGARVGVDAGRDAELYTVS
                     DSGVVVLGKNARALL"
     gene            1354..>2275
                     /locus_tag="VIJ44_01800"
     CDS             1354..>2275
                     /locus_tag="VIJ44_01800"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY5164662.1"
                     /translation="MPFFSGQGRRLIAWLRTLRGGALAVWGAAFVYYLFTQGLPLDRW
                     SQTLWILLALFAANVGRPWRSQLRILVDWLPFVGFLYVYDLTRGMADKLGTPVHVTQP
                     LAVEKWLFHGNVPTAWLQARFYQADTFHWWDIAVSLVYLSHFFVVWIYAAVLYIRSRD
                     EWAPWARRILMLSYAGLVTYVLYPSAPPWYANDHGLITGVSRIATRGWDPLGLHFAGV
                     LISQGQAQYNAVAALPSLHAAFTAMLTVFVWTRLRWWGRVLMTVYTLAMALSLVYGGE
                     HYVFDVLLGYLYVAVVLVVAWWWERWRSRRR"
BASE COUNT          328 a          782 c          775 g          390 t
ORIGIN      
        1 cgaagcagga gacgtcgacg tcggccagcc gacgcagctc gcggaccagg aagtccacgt
       61 ggacaccggc cccgccgtac acgtccgggg ggaactctcg ggtcatgatt ccaacgtgca
      121 cccgaccacc ctagggtgag cgcatggcga gccgcaggcg ggtactcggg attgttctgg
      181 caggtggtgc ggggaagcgg ttgatgcccc tgacggctga ccgagccaaa cctgctgtgc
      241 ccttcggcgg tcagtaccgg ctcgtcgact tcgtgctgtc caacctggtc aacgccggtt
      301 acctgcggct gtgcgtgctc acccagtaca agtcccactc gctcgaccgg cacatcacga
      361 cgacctggcg gatgtccacg ttgctgggca actacgtgac gccggtaccg gcccagcagc
      421 gcctcggccc gcgctggtac accggcagcg ccgacgcgat cttccagagc ctcaacctgg
      481 tgtacgacga ccagcccgac tacgtcgtcg tcttcggcgc cgaccacgtc taccggatgg
      541 acccgtccca gatggtccag gaccacatcg actccggtgc cggggtaacc gtggcgggca
      601 tccgcgtacc caaggcgcag gcgtccggct tcgggatcat cgagacggag gccaacggca
      661 aggacatcaa ggcgttcctg gagaagccgg ccgacccgcc gactgtccca ggtcaccccg
      721 acctctgcta cgcgtccatg ggcaactacg tcttctcgac cgacgtgctg atcgaggcgc
      781 tcaaggtcga ctccagcgac gagggctcgg tccacgacat gggtggcgac atcatcccga
      841 tgatgacgcg gctgggcgcg gccaaggtgt acgacttcgc cgacaacgac gttcccggcg
      901 cgaccgaccg cgaccgcggc tactggcgcg acgtcgggac gctcgactcg taccacgacg
      961 cccacatgga tctcgtgtcc gtgcacccga tcttcaacct gtacaaccgg cagtggccga
     1021 tcctgtcgca catgggcgca tggccacccg cgaagttcgt cgagggcggc gaggcgcacg
     1081 agtccatcgt gggcgccggg acgatcatct cgggcggtct cgtcgaccac tccgtcgtcg
     1141 cgttcgacgt acgcgtcggg cacggcgccg agatcaccga ctcggtgctc ttgccagggt
     1201 gcaccgtcgg ccgcggtgcc gtggtgcgcc gggcgatcct cgacaagggg gtcgtcatcc
     1261 cggacggggc acgggtcggc gtcgacgcgg gccgggacgc cgagctgtac accgtctcgg
     1321 acagcggggt cgtcgtgctc ggcaagaatg cgcgtgccct tctttagcgg ccaggggcgc
     1381 cggctgatcg cctggctcag gacgctgcgc gggggcgcgc tagcggtctg gggggcggcg
     1441 ttcgtctact acctgttcac gcaggggctg ccgctggacc gctggtccca gaccctgtgg
     1501 atcctgctcg cgctgttcgc ggccaacgtc ggccggccgt ggcgcagcca gctgcggatc
     1561 ctcgtggact ggttgccgtt cgtcgggttc ctgtacgtct acgacctcac ccgcgggatg
     1621 gccgacaagc tcggcacgcc ggtgcacgtc acccagccgt tggccgtcga gaagtggctc
     1681 ttccacggga acgtgccgac cgcctggctg caggcccgct tctaccaggc ggacacgttt
     1741 cactggtggg acatcgcggt gtccctcgtc tacctgtcgc acttcttcgt cgtctggatc
     1801 tacgcggcgg tcctgtacat ccggtcgcgc gacgagtggg cgccctgggc gaggcggatc
     1861 ctgatgctct cgtacgccgg cctggtcacg tacgtcctgt acccgtcggc accgccctgg
     1921 tacgcgaacg accatgggct gatcactggc gtctcccgga tcgccacgcg cggctgggac
     1981 ccactcggcc tgcacttcgc cggcgtcctg atcagccagg gccaggccca gtacaacgcg
     2041 gtcgcggcgc tgccgtcgct gcacgcggcc ttcaccgcga tgctcaccgt gtttgtgtgg
     2101 accaggctgc ggtggtgggg gcgcgtgctc atgaccgtct acacgctggc gatggcgctg
     2161 tcgctggtct acggcggcga gcactacgtc ttcgatgtgc tgctcggcta cctctacgtg
     2221 gccgtggtgc tcgtcgtggc gtggtggtgg gaacgctggc ggtcgcggcg gcggg
//
LOCUS       DATCCH010000089         3087 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_129324, whole genome shotgun sequence.
ACCESSION   DATCCH010000089 DATCCH010000000
VERSION     DATCCH010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129324"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1166
                     /locus_tag="VIJ44_01805"
     CDS             <1..1166
                     /locus_tag="VIJ44_01805"
                     /inference="COORDINATES: protein motif:HMM:NF023401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164663.1"
                     /translation="ALAGADYVVVTISTGGFDSMRHDLEIPARYGIRQPVGDSVGPGG
                     ITRALRSIPVLIKIAQAVERRCPDALLLNVTNPLSALCRAVTRQTDVPTVGLCNEVVG
                     LQFVTSLLLDADLRRVDPVVAGVNHLPLVTALRVEDEDGFARLRDLLNDEDAQRQPVW
                     MTPPPAMHYPKVSAGADWTKADVIAGNRLKLEIFTRFGVLPGSSDTHVAEFFPGFVTP
                     SSDYGREWSVHHYGLAGHMADKVADDADVAALLERDEISSYPSGELVAPLLDSLLSHR
                     PAALPVNLPNQGQVQNLPAGVVVECMGVATDDQRVVARDEAHVPSIPGEQLRRVAVSE
                     ELTVEAALSGNRTTVLEAMLADPMAGRLPYEHVVTMTDELLQSTARWLPQFAR"
     gene            1206..1979
                     /locus_tag="VIJ44_01810"
     CDS             1206..1979
                     /locus_tag="VIJ44_01810"
                     /inference="COORDINATES: protein motif:HMM:NF010663.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine reductase family protein"
                     /protein_id="HEY5164664.1"
                     /translation="MTSSPTSPPTFRALRAADPPPDLGRLYAPAWDRRETARHVRVNM
                     IASIDGGTAADGTSGSLGGPADGVIFALVRSFADVVLVGAGTMRAEHYGPARLDEEHR
                     QARVARGQTPVPPIAVITRSANLDWESEFFTQAEVRPIVLTVEASRDRHERAARVADV
                     ILAGAQEVDLAFALDELARRGYRDVLVEGGPSINAELAALDTIDELCLTISPLLLGGA
                     SSRIVRGDAAPPSRHLELVSSVTADGFLFLRYRRIPASP"
     gene            complement(2009..2614)
                     /locus_tag="VIJ44_01815"
     CDS             complement(2009..2614)
                     /locus_tag="VIJ44_01815"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY5164665.1"
                     /translation="MTTRLPAEQRRQQILDVACGVFADRGFHATAMDDIAAAAGVTKP
                     VLYQHFPSKRALFIELLGELGRRLLDELAGATATAHTGRERVEGGFAAYFRFVSGNEA
                     AFRVLFGAAARNDSEFADVIERLLDDVAVAISTLIDIEGTEEHRRVLAHALIGIAEAT
                     SRDTLTDGGSALDADRLARWVSELAWFGLRGVRAEEPARVV"
     gene            complement(2625..>3087)
                     /locus_tag="VIJ44_01820"
     CDS             complement(2625..>3087)
                     /locus_tag="VIJ44_01820"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY5164666.1"
                     /translation="LQTVFNVLEQEPGRTFASEPAVTEGRAGLISRVPHASDTLSGKI
                     DRGTEFPASDHRSHRNRDNMLDNFDKADTLAFLWEGSDRTIAQAAIAGILADPAFTTV
                     LPTVVSRDEVVEYAEASDKPLDNGEKARLNELWSRNFDHTNRYEMPLKSSA"
BASE COUNT          480 a         1159 c          976 g          472 t
ORIGIN      
        1 ccgcgctcgc gggcgccgac tacgtggtcg tgacgatctc gaccggcggc ttcgacagca
       61 tgcgccacga cctcgagatc cccgcccgct acgggatccg ccaacccgtc ggcgacagtg
      121 tcgggccggg agggatcacc cgtgcactgc gcagcatccc cgtcctgatc aagatcgccc
      181 aggccgtcga acggcgatgc cccgacgcct tgctgctcaa cgtcacgaac ccgctctcgg
      241 cactgtgtcg cgccgtcacg cgccagaccg acgtccccac ggtcggcctc tgcaacgagg
      301 tcgtcggtct ccagttcgtc accagcctcc tcctcgacgc tgatctccga cgcgtcgacc
      361 ccgtcgttgc cggcgtcaac cacctcccac tcgttaccgc actgcgcgtc gaggacgagg
      421 acggcttcgc acgactccgt gacctgctca acgacgagga cgcccagcgc caaccggtct
      481 ggatgacacc cccaccggcc atgcactacc cgaaggtctc cgcaggagcc gactggacca
      541 aggccgacgt gatcgccggg aaccgcctca agctcgagat cttcacccgg ttcggtgtgc
      601 tccccgggtc atccgacacc cacgtcgccg agttcttccc cggattcgtg acgccgagct
      661 ccgactacgg gcgcgagtgg tcggtgcacc attacgggct cgcgggccac atggccgaca
      721 aggtcgccga cgacgccgac gtcgccgcgc tgctggaacg ggacgagatc agctcgtacc
      781 cgtctggtga gctcgtcgcg ccgctcctcg attcgctcct ctcccaccga cccgcggcgc
      841 tgcctgtgaa cctgcccaac cagggccagg tccagaacct gcccgccggc gtcgtcgtcg
      901 aatgcatggg cgtcgcgact gacgatcagc gcgtcgtcgc ccgcgacgag gcgcacgttc
      961 cctcaatccc cggcgagcag ctgcgccgcg tcgcggtctc agaagagctc accgtcgaag
     1021 cagcgctcag cggcaaccgc accaccgtcc tcgaagcgat gctcgccgac ccgatggccg
     1081 gccggctccc ctacgagcac gtcgtcacga tgaccgacga actgctccaa tccaccgccc
     1141 gctggctgcc gcagttcgcc cgctgagtcg ctctcttcac cgtcatcgag ccagagccga
     1201 tccgcatgac ctcctccccg acctcaccac cgacgttccg ggcgcttcgc gccgcggacc
     1261 caccaccgga cctcggccgg ctctatgcgc cggcgtggga ccgccgcgag acagcccgcc
     1321 acgtccgggt caacatgatc gccagcatcg acggcggcac cgcagccgac gggacgtcag
     1381 gatccctcgg cgggcccgcc gacggcgtca tcttcgcctt ggtgcgctcc ttcgcggatg
     1441 tcgttctggt cggcgccggg accatgcgcg cggagcacta cgggccggcc cggctcgacg
     1501 aggagcacag gcaggcgcgc gtcgcgagag gtcaaacccc cgttccgccc atcgccgtca
     1561 tcacgcgcag cgccaacctc gactgggaga gcgagttctt cacccaggca gaggttcgcc
     1621 cgatcgtctt gaccgtcgag gcatcccggg atcgccacga acgcgcggcg cgggtcgccg
     1681 acgtcatcct cgccggggca caagaagtcg acttggcgtt cgctctcgac gagctggcgc
     1741 ggcgcggata ccgggatgtg ctcgtcgaag gcggccccag catcaacgcc gagctcgccg
     1801 cgctcgacac catcgacgag ctctgcctga ccatctcgcc cctcctgctt ggcggcgcga
     1861 gctcccggat cgtgaggggg gacgcggcac cgccaagccg ccacctcgag ctggtctcga
     1921 gcgtcactgc cgacggcttc ttgttcctcc gctaccgacg catcccggcc agcccgtagc
     1981 cgtcgtcacc tcggtcgcgc accggcggtc agacgacccg ggccggctcc tcggcgcgga
     2041 ccccacggag gccgaaccac gcgagctctg acacccaccg ggcgagccgg tccgcgtcga
     2101 gggcggaacc cccgtcggtg agggtgtcgc ggctcgtcgc ctcagcgata ccgatcaacg
     2161 cgtgagccag cacccgtcgg tgctcctccg ttccttcgat gtcgatgagc gtgctgatcg
     2221 ccaccgccac atcgtcgagg agacgctcga tgacgtcggc gaactcggag tcgttgcgcg
     2281 ccgcggcgcc gaacagaacc cgaaacgccg cctcgttccc ggacacgaac cggaaatagg
     2341 cggcgaaacc cccctcaact cgctcgcggc cggtgtgggc cgtcgccgtc gctcccgcga
     2401 gctcgtcgag cagcctgcgc ccgagctcgc cgagcaactc gatgaacaac gcccgcttgg
     2461 aagggaaatg ctgatacagg accggcttgg tgacaccggc ggccgcggcg atgtcgtcca
     2521 tcgcggtggc gtggaaaccc cggtccgcga acacgccgca cgcgacgtca aggatctgtt
     2581 ggcgccgctg ctcggccggg aggcgggtcg tcaccgggcc cgcctcacgc actcgacttc
     2641 aagggcatct cgtaccggtt ggtgtgatcg aagttccggg accacagctc gttgagccga
     2701 gccttctcac cgttgtcgag cggcttgtcc gaagcctcgg cgtactcgac gacctcgtcc
     2761 cggctgacca cggtcgggag gacggtcgtg aacgccgggt cggcgaggat cccggcgatg
     2821 gccgcctgag cgatggttcg gtcgctccct tcccacagga acgccaaggt gtcagccttg
     2881 tcgaagttgt cgagcatgtt gtcgcgattg cggtgcgatc ggtggtcgct ggcggggaac
     2941 tcggtgcccc ggtcgatctt tcccgacaag gtgtccgagg cgtgggggac ccgcgagatg
     3001 agccccgctc gaccctcggt cacggccggt tccgaggcaa aggtcctacc cggttcctgt
     3061 tcgaggacgt tgaagacggt ctggagc
//
LOCUS       DATCCH010000090         2704 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_507802, whole genome shotgun sequence.
ACCESSION   DATCCH010000090 DATCCH010000000
VERSION     DATCCH010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507802"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VIJ44_01825"
     CDS             complement(<1..295)
                     /locus_tag="VIJ44_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164667.1"
                     /translation="MDPTRVDGIDWPVVLRGATAGLSVIVPVTILRAILDRDVTDFNN
                     SSWVYPLFILLLAAYVLAGWVAGRGAPEAPLTHGALAGLGSVVLWIPIRIVIWV"
     gene            558..800
                     /locus_tag="VIJ44_01830"
     CDS             558..800
                     /locus_tag="VIJ44_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEY5164668.1"
                     /translation="MAMRAIGSWRDQARCRGVDPEIFHPGEDDDGVEAKAICTQCPVR
                     EACLEYALATREKEGVWGGLTARERRRVLRQRRKSA"
     gene            857..2023
                     /locus_tag="VIJ44_01835"
     CDS             857..2023
                     /locus_tag="VIJ44_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY5164669.1"
                     /translation="MQFDLSPELAELQATVRKLAQERIAPRAREIDDSEEYPQDLFEL
                     FVDAGLTGLCVPEELGGSGAGIFGLALAIEEVAKYSNAAALMLLLTRLPTGPVLIAGS
                     EEQQRRFVTPVATGEARAAFGLSEPQAGSDVMGMRTRARADGDDWILTGTKCWMSGVR
                     QADWYCIFAKTGPVDSRAHDSVSAFIVERDRPGVAVGSIDRKMGVKGVDTGELLLDDV
                     RVPAENVVGEIGGFRLAMLGLNAMRPIVAARGIGLAEGALMYAVEYVQNRQAFERTIA
                     DFQGIQWKIAELATEIEAARLLTYRAAWMADRGQFTKEWVPFLSMAKYYATELGVKVS
                     GEALQMLGAAGYMKDHPLELYYRDAKQLTIVEGTSQIQLGLIARGVLDHDLWWD"
     gene            2062..>2704
                     /gene="trpD"
                     /locus_tag="VIJ44_01840"
     CDS             2062..>2704
                     /gene="trpD"
                     /locus_tag="VIJ44_01840"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEY5164670.1"
                     /translation="MTDSPVLWTEVLGRLVRREELPASLAEAALGAVLAGDATDAQIA
                     AFAVALRAKGETPAELAALVRAMLAHADHVDWDGPEPLVDTCGTGGDQAGTINVSTIA
                     ALIAAGGGACVAKHGNRAASSRCGSADVLEALGVAIDLGPAGVTRCLRDARIGFCFAQ
                     RFHSALRHAAVARRELGVPTTFNFLGPLANPAGATRRTVGVSDPAMAERVVGAL"
BASE COUNT          423 a          959 c          927 g          395 t
ORIGIN      
        1 ccacccagat cacgatccgg atcgggatcc acagcaccac cgagcccaac ccggcgaggg
       61 caccgtgggt gagcggtgcc tccggcgctc ctcgccccgc gacccagccg gccaggacat
      121 acgccgcgag gagcaggatg aacagcggat acacccagct gctgttgttg aagtcggtga
      181 cgtcccggtc gaggatcgcc cggaggatcg tgactggcac gatgaccgac aacccggccg
      241 tcgcgccgcg caggaccacc ggccagtcga tgccgtcgac acgggttggg tccatcgggg
      301 ctcctccacc ccgcaatcgc agggcgcacc cgatcatcgc acccgccgtc cccggggttc
      361 ggtctcaacc cggggtgggc gtcgccgccc gaaacacccg ccagcgctcc gggatccgcc
      421 gccgcgccgg gcaccaccgg gccccgacta ccgggttcgg gtcggctcag gtggtgggga
      481 atactcgtcg atgcactcat cgttgaacta gacccccgtc ccccccgggg gtgtggacgt
      541 agaaagcgtg gtgccccatg gccatgcggg caatcggctc ctggcgtgac caggcccgct
      601 gtcggggtgt cgaccccgag atcttccatc ctggcgaaga cgacgacggc gtcgaggcga
      661 aggcgatttg cacccagtgc ccggtccggg aggcctgcct ggagtacgcc ttggccaccc
      721 gagagaagga aggcgtctgg ggtggcctga ccgcccgcga acgccgccgg gtgctccgcc
      781 agcgccgcaa gagcgcgtaa ccggtccggg ccgccggcgc cgagagggcc ccaggaccgc
      841 ccggtaccct caggcggtgc aattcgacct ctcgccggag ctcgccgagc tccaggccac
      901 ggtccgcaag ctcgcccagg agcgcatcgc gccccgagcc cgcgagatcg acgactccga
      961 ggagtacccc caagacctct tcgagctgtt cgtcgacgcg ggcctcaccg ggttatgtgt
     1021 cccagaggag ctgggcgggt cgggcgccgg gatcttcgga ctggccctgg cgatcgaaga
     1081 ggtcgccaag tactcgaacg ccgcggccct gatgctgctc ttgacccggt tgcccaccgg
     1141 tccggtgttg atcgccggca gcgaggagca gcagcggcgc ttcgtgactc ccgtcgcgac
     1201 cggggaggcc cgggccgcgt tcgggctgtc cgaaccccaa gccggcagcg acgtgatggg
     1261 tatgcgtacc cgggctcgcg ccgatgggga cgactggatt ctcaccggga ccaaatgctg
     1321 gatgtcgggc gtccgccaag ccgactggta ctgcatcttc gccaagaccg gacccgttga
     1381 ttcccgggcc catgacagcg tctcggcgtt catcgtcgag cgcgaccgtc ccggcgtcgc
     1441 ggtcgggtcc atcgaccgca agatgggtgt caaaggggtc gacaccggcg agttgctcct
     1501 ggacgacgtg cgcgtccccg ccgagaacgt ggtgggagag atcggtggct tccgcctcgc
     1561 gatgctcggg ctgaacgcca tgcggcccat cgtcgcggcg cgaggcatcg ggctcgccga
     1621 aggtgccctc atgtacgcgg tcgagtacgt tcagaatcgt caagcgttcg aacggacgat
     1681 cgccgacttt caaggcatcc agtggaagat cgcggagctc gcaaccgaga tcgaggcagc
     1741 ccgactgctc acctatcgag ccgcgtggat ggccgaccgg ggacagttca ccaaggaatg
     1801 ggtcccgttc ctctcgatgg ccaagtacta cgccaccgag ctcggggtga aggtgtccgg
     1861 cgaggcgctc cagatgctcg gcgcggccgg ctacatgaaa gaccatccgc tcgagctcta
     1921 ctaccgcgac gccaaacagc tcacgatcgt cgagggcacc agccagatcc agctcgggct
     1981 gatcgcccgt ggtgtgctcg accacgacct ctggtgggac tagtcggtgc gacgctgcca
     2041 tcggactcga cggtctcggc gatgaccgat tccccagtgc tgtggaccga ggtgctcggt
     2101 cgcttggtgc gacgagaaga gctccccgcc tccctcgctg aggccgcgct cggcgcggtc
     2161 ctcgcgggag acgccaccga cgcgcagatc gcggcgttcg cggtcgcgct gcgagccaaa
     2221 ggtgaaacgc cggccgaact cgcggcgctg gtccgagcca tgctcgccca cgccgaccac
     2281 gtcgactggg acggtcccga accgctcgtc gacacctgcg ggaccggcgg cgaccaggcg
     2341 ggcacgatca acgtctcgac catcgccgcg ctcatcgccg ccggtggtgg cgcctgtgtg
     2401 gccaaacatg ggaaccgggc ggcgtcctcg cgctgcgggt cggcggacgt cctcgaagcg
     2461 ctcggggtcg cgatcgacct cgggcccgcc ggtgtgaccc gctgcctccg agacgccaga
     2521 atcggcttct gcttcgcgca gcggttccat tccgcgctcc gccacgccgc cgtggcccga
     2581 cgcgagctgg gcgtgcccac gacgttcaac ttcctgggcc cgttggcgaa cccggcggga
     2641 gcgacacgcc gcaccgtcgg cgtctcggac cccgcgatgg cggagcgggt tgtcggcgcc
     2701 ctcg
//
LOCUS       DATCCH010000091         2857 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_46555, whole genome shotgun sequence.
ACCESSION   DATCCH010000091 DATCCH010000000
VERSION     DATCCH010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46555"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="VIJ44_01845"
     CDS             <1..549
                     /locus_tag="VIJ44_01845"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY5164671.1"
                     /translation="LDSYPIHPGNDGYLLFLGRMSPDKGAGNAVRVALETGMPLKLAG
                     KIHDPIEHEYFDSAVRPYLGNGIEYVGEVSHNEKVELLQHAAVTVFPIQWPEPFGLVM
                     VESMACGTPVLATRLGAVPEVVEHGRGGLVVDTISEMAACMPRMLALDPLEVRMVAEE
                     SFSAARMVADYERAYERLLAEA"
     gene            complement(613..2151)
                     /gene="purH"
                     /locus_tag="VIJ44_01850"
     CDS             complement(613..2151)
                     /gene="purH"
                     /locus_tag="VIJ44_01850"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEY5164672.1"
                     /translation="MKIKRALISVSDKNGLSTLATGLHALGVELISTSGTAGFLEQAG
                     VPVTTVERLTGNPELLGGRVKTLHASLHAAILARRDHPEDMASLEQAGITPIDLIVCN
                     LYPFRSVAARRGVAEAEVIANIDIGGPTMLRAGAKNFHSVAVVTEPDRYGFLLDELNS
                     AGGELSLDTRRDLAGEAFAHVASYDSAVAAWFTDTEPFPDRLTIDLVKVGDLAYGENP
                     HQRAALYRETGSRRHVLSRIDQVSGKPLSFNNVADLQAARAIVAAFQLPACAIVKHAG
                     PCGVAVGATLEEAYVRALAADPMSAFGAVIAVNRSVTADVAERIIETKVDVLFAPGYD
                     DAARETLSSKPTTRVLEDRERRKSSPGERDLRRVLGGLLIQDRDNETEDRADMEVVSE
                     VAPGKREWEDILFAWRTVHFVRSNAVVIAHDLATVGIGAGQTSRVDAVKVAVSKAGER
                     AAGAVLASDGFFPFADGPEAAFAAGVRAIVQPGGSVRDAEVIAAANSAGAAMVFTGRR
                     HFLH"
     gene            complement(2148..2699)
                     /gene="purN"
                     /locus_tag="VIJ44_01855"
     CDS             complement(2148..2699)
                     /gene="purN"
                     /locus_tag="VIJ44_01855"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006694314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEY5164673.1"
                     /translation="MLISGVGTNLQTLIDAPDIDVACVVSSRADAPGLDRARRAGIPA
                     LASADEDETSAFLDQNRVGLVVLAGYMRILSPAFVRAYSGRILNVHPSLLPAFPGAHA
                     VEDALAHGVKVTGVTVHLVDDDHPSVDSGPIVLQESAAVSYDDTVADLTERLHRIEHR
                     LLPEAVRLYRAGKIQVDGRQVSG"
BASE COUNT          430 a         1003 c          991 g          433 t
ORIGIN      
        1 ctcgacagct atccgatcca tcccggcaac gacggctacc tcctgttcct cggccggatg
       61 tcccccgaca agggagccgg caacgcggtg cgagtcgcgc tcgagaccgg gatgcctctg
      121 aagctggcgg gcaagatcca cgatccgatc gagcacgagt acttcgactc cgccgtgcgg
      181 ccgtatctgg ggaatggcat cgagtacgtc ggcgaggtct ctcacaacga gaaggtcgaa
      241 ctcctccagc acgccgccgt gacggtgttc ccgattcagt ggccggagcc ctttggactc
      301 gtgatggtgg agtcgatggc gtgcgggacg ccggtgctgg ccacccgtct tggtgcggtg
      361 cccgaggtcg tcgagcacgg ccggggcggg ttggtcgtgg acacgatcag cgagatggcg
      421 gcctgcatgc cgcggatgct cgcgctcgac ccgctcgagg tgcggatggt ggccgaggag
      481 agtttctctg cggcgcgaat ggtggccgac tacgagcgcg cctacgagcg cctcctcgcc
      541 gaggcgtaac aagactcagc ttctcgcacg gcagctttcg ggggactgtc cccggttctt
      601 cgtccccggt tcctagtgca ggaagtggcg gcggcccgtg aagaccatgg ccgcgccggc
      661 ggagttcgcg gcggcgatca cctcggcgtc gcgcaccgag ccgcccggct ggacgatcgc
      721 ccggacgccc gcggcgaacg ccgcctcggg accgtcggcg aacgggaaga acccgtcgga
      781 ggccagcacg gcgccggccg cccgttcgcc cgccttcgag accgccacct tgacggcgtc
      841 cacgcgactc gtctgtccgg cgccgatccc gaccgttgcc aggtcatggg cgatcacgac
      901 cgcgttcgag cgcacgaagt gcacggtccg ccaggcgaac aggatgtcct cccactcgcg
      961 cttgccgggc gccacctcgg acacgacctc catgtccgcg cgatcctcgg tctcgttgtc
     1021 gcgatcctgg atcaggaggc cgccgagcac ccgccgcaga tcgcgttcgc cggggctcga
     1081 cttgcgccgc tcgcggtctt cgagcacgcg cgtcgtcggc ttggacgaga gcgtctcacg
     1141 ggcggcatcg tcgtatcccg gcgcgaacag cacgtcgacc ttcgtctcga tgatgcgctc
     1201 tgccacatcg gccgtgaccg acctgttcac ggcgatcacg gccccgaacg ccgacatcgg
     1261 gtcggcggcg agcgcgcgca cgtaggcctc ctcgagcgtc gcaccgaccg ccacgccgca
     1321 ggggccggcg tgcttgacga tggcgcacgc aggcaactgg aacgcggcga cgatggcgcg
     1381 ggccgcctgc aggtcggcga cgttgttgaa cgacagcggc ttgccgctca cctgatcgat
     1441 gcgcgagagc acgtgccggc gcgagcccgt ctcgcggtag agcgccgctc gctggtgcgg
     1501 gttctcgccg taggcgaggt cgccgacctt gaccaggtcg atcgtgagcc ggtcgggaaa
     1561 cggctcggtg tcggtgaacc aggcggccac agcagagtcg taggacgcca catgcgcgaa
     1621 cgcctctccg gccaggtcgc ggcgcgtgtc cagtgacagc tcgccacccg cggagttcag
     1681 ctcgtcgagc aggaacccgt accggtcggg ctcggtgacg acggccaccg agtggaagtt
     1741 cttcgcgccg gcgcggagca tggtcgggcc gccgatgtcg atgttggcga tcacctcggc
     1801 ctcggccacg ccccggcggg cggccaccga ccggaaggga tacaggttgc agacgatcag
     1861 gtcgatgggc gtgatcccgg cctgctcgag cgacgccatg tcctcgggat ggtcgcgccg
     1921 ggcaaggatg gccgcgtgga gcgaggcgtg cagcgtcttc acccgtccgc cgagcagctc
     1981 gggattgccc gtcagccgct cgacggtcgt cacggggacg ccggcctgct cgaggaaccc
     2041 ggccgtgccc gacgtcgaga tcagctcgac cccgagcgcg tgcagacccg tcgccagcgt
     2101 cgagaggccg ttcttgtcgc tgaccgatat cagggctcgt ttgatcttca tccgctcacc
     2161 tgccgtccgt cgacctggat cttgcccgcc cggtacaacc tcaccgcttc cggcagcaac
     2221 cgatgttcga tccggtgcag acgctccgtc aggtcggcga cggtgtcatc atacgaaacc
     2281 gccgcgctct cctgcaggac gatcggcccg ctgtccacgc tcgggtggtc atcgtcgacg
     2341 aggtgcaccg tgacgccggt gaccttgaca ccgtgtgcga gagcgtcctc cacggcgtgg
     2401 gcgcccggaa acgcgggcag gagcgaggga tgcacgttca ggatccggcc cgagtaggcg
     2461 cgcacgaacg cgggcgagag aatgcgcatg tagccggcca gcaccaccag tccgacccga
     2521 ttctggtcga ggaacgcgct cgtctcgtcc tcgtcggcgc tcgcgagggc cggaatgccc
     2581 gcccgccggg cgcggtcgag ccccggcgcg tctgctcggc tcgagacgac gcaggccacg
     2641 tcgatgtcgg gagcgtcgat gagcgtctgg aggttggtgc cgacccccga gatgagcacg
     2701 ccgaccctca gcggaagcgc acccccgccc cctccaccac gctcccggcg acgaagctcg
     2761 ggcagccgcc ccggtcgagc gcggtcagcg cggtcggcgc cgagtccatc tcgatcacgg
     2821 ccgcatatcc gatgccgagg ttgaacacgc gccgcat
//
LOCUS       DATCCH010000092         2041 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_467005, whole genome shotgun sequence.
ACCESSION   DATCCH010000092 DATCCH010000000
VERSION     DATCCH010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467005"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="VIJ44_01860"
     CDS             <1..675
                     /locus_tag="VIJ44_01860"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEY5164674.1"
                     /translation="IGWGIPDAAVAFVLSLVAASIAVLPFFSHSKLPKHLEVDASFAS
                     LLAQTGTVIGYLVFAARKGRGSLTADFGLRVRVRDGLWFGVGFVLSIASGILIAPIVS
                     AGHLSNSSQDVVRLFQQARGVETVFFAFAVLVVAPLGEELLFRGALLRGLLRRTSPVV
                     AIWGSGLAFALVHVALDPGAGFAVPALLGLGLLSSYRALRTGDLSQSIMLHAGFNLLA
                     VLQILT"
     gene            978..>2041
                     /locus_tag="VIJ44_01865"
     CDS             978..>2041
                     /locus_tag="VIJ44_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164675.1"
                     /translation="MAATTVRDGPAAAPDDAEPPAPAWWARLEIWVGLLIVVACCVFI
                     FVQLDPRLLLRNTTASGGDTGAHVWWPAYLRDHLLPWRLSGWSPDFYAGFPAGQFYFP
                     VPALLIVGLNLFIPYNVAFKLVTALGPMLLPVGAYVFARGIRAPNPAPAALAVAATGF
                     LFFNGDPGTTQADKGIAFNQHIMGGNLASNLAGEFSFTLALAFALLFLGTLAWALRTR
                     RAPWLPAVLLAATMMSHLVVAVFALLGAAVVFVGARPRPTVPHPATRRAILGGVLAAG
                     GILLAVMSGSYLAGGLLVVVGLGIVVFQIWPAATVTRAAAIGAVGALLTAVWSVPLVA
                     TLQYTTDMRYGSIGVSKDSAT"
BASE COUNT          229 a          791 c          640 g          381 t
ORIGIN      
        1 atcgggtggg gcatccccga cgcggccgtc gcctttgtcc tctcccttgt cgcggcgtcg
       61 atcgcggtcc tcccgttctt cagccacagc aagctcccca agcacctcga ggtcgacgcc
      121 tccttcgcat cgctgctggc ccagaccggc accgtcatcg gctatctcgt gttcgccgcc
      181 cgcaagggcc gaggcagcct caccgctgac ttcgggttgc gggtccgggt ccgcgacggc
      241 ctgtggttcg gcgtcgggtt cgtgctgtcg atcgcgtcgg ggatcctgat cgcgccgatc
      301 gtctccgccg ggcacctcag caactcctcc caggatgtgg tgcgactctt ccagcaggcc
      361 cgcggcgtcg agaccgtgtt cttcgcgttc gcggtgctgg tcgtcgcgcc gctcggtgag
      421 gagctcctat tccggggggc gctcctgcgc gggctgctgc gtcgcaccag cccggtggtg
      481 gcgatctggg gctcaggcct ggcgttcgcg ctcgtccatg tcgccctcga ccccggggcc
      541 gggttcgcgg tgcccgccct gctcggcctc gggctcctct cgagctatcg ggctctccgc
      601 accggtgacc tgtcccaatc catcatgttg cacgcgggct tcaacctcct cgccgtcctc
      661 cagatcctca cctgagctca agtccagccc ggacctgccg ataccgggca ttaggaagag
      721 ctgggaggct gttatgcgct gtccgtcgtg cagcaaaggg tcgatcatcg agatccggat
      781 ggaaatcggt ggtgaggacc tcacgttccg acggtgtggt cgctgtgagt tgcaatcctg
      841 gaccaacccc ggcggccgtg tgcccctcag tcgcgtgctc gagctggccc gcaccacctg
      901 aacccatctg actcgggctc caccgtcgcg tcgacggtga tgccccgatc cccgccagcc
      961 atgtcaggct aaggcccatg gccgcgacga cagtgcggga cggccccgcc gcggcccccg
     1021 acgacgccga accccccgct ccagcctggt gggcgcgcct cgaaatctgg gtcgggttgc
     1081 tcatcgtcgt cgcctgctgc gtcttcatct tcgtccagct cgacccccga ctcctgctcc
     1141 gcaacaccac cgcctccggc ggcgacaccg gcgcgcacgt ctggtggccc gcctacctgc
     1201 gcgaccacct gctgccgtgg cgcctctcgg gctggagtcc cgacttctac gctgggttcc
     1261 ccgccggtca gttctacttc ccggtgccgg cgctgctcat cgtcgggctc aacctcttca
     1321 tcccctacaa cgtcgcgttc aagctcgtca ccgccctcgg cccgatgctc cttcccgtcg
     1381 gtgcctacgt gttcgcccgc gggatccggg ctccgaaccc cgcgccggcg gcgctggccg
     1441 tcgccgcgac cggcttcctc ttcttcaacg gcgaccccgg caccacccag gccgacaagg
     1501 gcatcgcgtt caaccagcac atcatgggcg gcaacctcgc cagcaacctc gccggggagt
     1561 tctcctttac cctggcgctg gcgttcgcgc tgctgttcct cgggaccctg gcgtgggcgt
     1621 tgcgcacccg ccgagccccg tggctccctg ccgtcctgct cgcggcgacg atgatgagcc
     1681 acctcgtcgt ggcggtcttc gcccttctcg gcgccgccgt cgtgttcgtc ggggcgcggc
     1741 ctcgcccgac ggtcccgcac cccgcgacgc ggcgcgccat cctcggcggt gttctcgccg
     1801 ccgggggcat cctgctcgcg gtcatgtcgg gcagctacct cgccggcggc ctgctcgtcg
     1861 tcgtcggcct cgggatcgtc gtcttccaga tctggcccgc cgcgacggtc acccgcgctg
     1921 ccgcgatcgg cgctgtcggc gcgctgctca ccgcggtgtg gagcgtcccg ctcgtcgcga
     1981 cgctgcagta cacgaccgac atgcgctacg ggtccatcgg ggtcagcaag gacagcgcga
     2041 c
//
LOCUS       DATCCH010000093         2006 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_378730, whole genome shotgun sequence.
ACCESSION   DATCCH010000093 DATCCH010000000
VERSION     DATCCH010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378730"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..758
                     /locus_tag="VIJ44_01870"
     CDS             <1..758
                     /locus_tag="VIJ44_01870"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEY5164676.1"
                     /translation="EPGAAADPIAVHAHATRDGGAWTLSGTKTFVLDGDTADEIVVVA
                     REPGTVGEDGVAAFVTPRDALRVTPRRPLDPTRSLVDLDLDGARVSDDRRLGDPGPHT
                     VLALRRAIEEATAMLAVEIVGSSQAILDVTLDYAKQREQFGLPIGSFQAIKHKFADML
                     IALERARATAYFAALCVAEDDPRRAIAVATAKAAAGDCQRRFGKEGIQILGGIGYTWE
                     HDMHLYVRRIKTDAQLLGTAHEHRRRIAELLGL"
     gene            complement(816..1586)
                     /locus_tag="VIJ44_01875"
     CDS             complement(816..1586)
                     /locus_tag="VIJ44_01875"
                     /inference="COORDINATES: protein motif:HMM:NF017945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HEY5164677.1"
                     /translation="MLHLAVNIFNRTPGLEFLARWAHVLVGILWIGLLYYFNFVQVPS
                     FAQMDAGARNNAIDKLASRALWWFRWAAAATLGFGIVLLLVIPGSNGNSELFSGVYWK
                     SAQGISIATGILLGVTMFLNVWLVIWPQQKKVIANARNVQAGGQADPAAADAGRRAAL
                     ASRQNTIFSIPMLFFMVGTAHFFTAGLYDTSKASDRGIYFAITAVIWAVFELSALGII
                     GGRAAGNITNWVYENHWRAIGSGLVLVVVWYALFEIMF"
     gene            complement(1715..>2006)
                     /locus_tag="VIJ44_01880"
     CDS             complement(1715..>2006)
                     /locus_tag="VIJ44_01880"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEY5164678.1"
                     /translation="DVAAGSCAGRSSSFDPRDQSLVTADLHVRYLGRPKGSPVRAEAR
                     VMRSGRMLIVVEVRVLDPLDNLIAFADFSAMIVARREPLDGVVPTDPQAPDL"
BASE COUNT          360 a          688 c          671 g          287 t
ORIGIN      
        1 ccgagccggg tgcggccgcc gacccgatcg cggtgcatgc ccacgcgacg agggacggtg
       61 gagcgtggac gctgtcgggc acgaagacct tcgtcctcga cggtgacacg gccgacgaga
      121 tcgtcgtggt ggcccgcgag cccgggacgg tcggcgagga cggcgtcgcc gcgttcgtca
      181 cgccccgtga cgcgcttcga gtcactcccc ggcgccccct cgacccgacc cgcagcctcg
      241 tcgatctgga cctcgacggc gcccgggtgt ccgacgaccg acggctcggc gaccccgggc
      301 cgcacaccgt gctggcgttg cggcgagcca tcgaggaagc caccgccatg ctcgcggtcg
      361 agatcgtcgg ctcctcccag gcgatcctcg acgtgacgct cgactacgcg aagcagcgcg
      421 agcagttcgg cctgccgatc ggttcgttcc aggcgatcaa gcacaagttt gcggacatgc
      481 tcatcgccct cgaacgggct cgggccaccg cgtacttcgc cgccctttgc gtcgccgagg
      541 acgacccccg gcgcgcgatc gccgtcgcca cggccaaggc cgctgcgggc gactgtcagc
      601 gacggttcgg gaaggaaggc atccagatcc tcggcggcat cgggtacacg tgggagcacg
      661 acatgcacct ctatgtgcgc cgcatcaaga ccgatgccca gctgctcggg accgcccacg
      721 agcaccgtcg ccgcatcgcg gagctgctcg ggctctgacg cggaaacgcc cggctcacgc
      781 cgggcgcctc catcacatga gtggtgtcgg gactctcaga acatgatctc aaagagggcg
      841 taccagacca cgacgagcac gagaccgcta ccgatggccc gccagtggtt ctcgtacacc
      901 cagttcgtga tgttgccggc cgcccggcca ccgatgatcc cgagcgcgct cagctcgaac
      961 acagcccaga taaccgcggt gatcgcgaag tagatgcctc gatcgctcgc cttcgacgtg
     1021 tcgtagagcc cagcagtaaa gaaatgcgcg gtgccaacca taaagaacag catcgggatc
     1081 gagaagatgg tgttctggcg tgacgccagc gcggctcggc ggccagcatc ggccgcggcg
     1141 gggtcagcct ggcctccggc ttggacgttg cgagcgttgg cgatgacctt cttttgctga
     1201 ggccagatga cgagccagac gttgaggaac atcgtcacgc ccagcaagat gccggtcgcg
     1261 atgctgatcc cctgggcgct cttccagtag acaccgctga acagctcgga gttgccgttg
     1321 ctgcccggga tgacgaggag cagcacgatc ccgaacccga gcgtcgcggc cgcggcccaa
     1381 cggaaccacc acagcgcccg agaggcgagc ttgtcgatgg cgttgtttcg ggccccggcg
     1441 tccatctgcg cgaacgaggg cacctgcacg aagttgaagt agtagagcag cccgatccag
     1501 aggatcccga cgagcacatg cgcccatcgg gctaagaact ccagcccggg tgtgcggttg
     1561 aagatgttca cagccaggtg caacatcggt gggccccctt gtggtgtgcg cgaagtcggc
     1621 ggcaacgtag cacccaaaca cggcatcgac tggctcgacc gcacgacgcc gaccgggtcg
     1681 ggccctatcc ccaggcacga cccggggcga aggatcagag gtccggggcc tgggggtcgg
     1741 tggggaccac cccgtcgagc ggttcccgcc gcgcgacgat catcgccgag aagtccgcaa
     1801 acgcgatcag gttgtcgagc gggtccagaa cacggacttc aaccacgatg agcatgcgcc
     1861 ccgagcgcat cacccgagcc tcggcccgga ccgggctccc cttcgggcga cccaagtatc
     1921 ggacgtgcag gtcggcggtg acgaggctct ggtctcgggg gtcgaagctc gaggaccgac
     1981 cggcgcagga cccggcggcg acatca
//
LOCUS       DATCCH010000094         4898 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_129490, whole genome shotgun sequence.
ACCESSION   DATCCH010000094 DATCCH010000000
VERSION     DATCCH010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4898
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129490"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..107)
                     /locus_tag="VIJ44_01885"
     CDS             complement(<1..107)
                     /locus_tag="VIJ44_01885"
                     /inference="COORDINATES: protein motif:HMM:NF024744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HEY5164679.1"
                     /translation="MSDRRVTLAGPLNFRDLGGYPTVDGRTVRWGQIYR"
     gene            complement(104..784)
                     /locus_tag="VIJ44_01890"
     CDS             complement(104..784)
                     /locus_tag="VIJ44_01890"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEY5164680.1"
                     /translation="MNGQPNIVDGHGPDASSPPGPELARLTEALRHSTDLVVRPGASP
                     VMLAEAAALVERAADLLEAATRPWTPSVPARPLAELEPHDFFPFSPMIGLFSPLAPPI
                     HCERHEGGVRGHGLLGAAYEGPPGCVHGGIIAGLFDELLGVANIVAGVGAMTGTLTVV
                     YRSPTPLYTELRLAARTLGVDGRKVRTVGTLHAGDRLCAEAEGVFILVDHARFAAHTL
                     AHGAGKNP"
     gene            complement(818..1018)
                     /locus_tag="VIJ44_01895"
     CDS             complement(818..1018)
                     /locus_tag="VIJ44_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEY5164681.1"
                     /translation="MAWKVVVDFDVCESNAICMGVAPEVFEVRDDDFLYVLQEEPPDD
                     LRPKVEQAVRQCPKQAISIAED"
     gene            complement(1051..1812)
                     /locus_tag="VIJ44_01900"
     CDS             complement(1051..1812)
                     /locus_tag="VIJ44_01900"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY5164682.1"
                     /translation="MRLLTLDRPEARNALDTVHYHALADAFDDAATRTDVAVVALTGS
                     AGAFCAGQDLAEMGRLGDRSATTDEARNGHGFTRFVGALESFPKPVVAAVNGVAVGLG
                     VTMLPYCDLVLVADDARFRAPFASLGVVPEAGSSFTLPVAMGRQVAAHALLTGAWFDA
                     ATAVASGLAWKSCPAALLLDEALDAARAMARLPIVSLSETKRLMRAGWLSAAQAARAG
                     EEDVFARLTGAPANREAIAAFLEKREPDFVNLPPE"
     gene            complement(1840..2295)
                     /locus_tag="VIJ44_01905"
     CDS             complement(1840..2295)
                     /locus_tag="VIJ44_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY5164683.1"
                     /translation="MTDARSAIERIVYGYADRVDMGDFAGLAELFRHATYKGGGPDDP
                     GVVGANAVLAIQERMVRRYPDGTPRTKHVTTNLVVDADEGAGTAAAHSYFTVLQQVDD
                     FPLQAIIAGRYHDQFERVAGEWRLTERVIFCDLIGDLSHHLTIDPFGAR"
     gene            complement(2312..2620)
                     /locus_tag="VIJ44_01910"
     CDS             complement(2312..2620)
                     /locus_tag="VIJ44_01910"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEY5164684.1"
                     /translation="MSKVSVIATLTVKDGKADEFPAAFDEFFRHVETEDGTEEYVLHR
                     STTNPNVFYMTELYTNKGAFDAHAGSDAFAKLGASMGDYIEGFDLQLAEPLKAKGADR
                     "
     gene            complement(2634..4358)
                     /locus_tag="VIJ44_01915"
     CDS             complement(2634..4358)
                     /locus_tag="VIJ44_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5164685.1"
                     /translation="MHDLVIRGGTIVDGTGADAAPGDVAVDDGRITAVGRVDAEGDEV
                     IDATGCVVTPGFVDVHTHYDGQVTWDPTLAPSSWHGVTTVVMGNCGVGFAPAKPDRRD
                     WLIGLMEGVEDIPGSALSAGIRWGWETFPEFLDFLDAQPLAIDVATQVPHGAVRGYVM
                     GERGARNEPATPDDIAEMAKLVREGVEAGAVGVSTSRTIAHRAIDGEPVPGTFAAEEE
                     LFAMGRALGEAGSGVFELAPAGVMGEDLAASDREMDWMRRLSRETSRPVTFALLQHDH
                     DPDQWKRMLDLALEAYDDGVPVRPQVAGRPLGLLLGLQTFHPLRDRPTYQALDHLDLD
                     EKVARLREPDVRAAILSERAPKREGIEAYIGLGLDRIFRLGEPPDYEPAPEESIAAIA
                     ERDGRDKWDLFYDLLLENDGRELLLRPLLGYSNFTQDPIREMVMHPTSALGLGDGGAH
                     VGSICDASIETYMLTHWVRDRTRGERLPLELVVRKMTSDTAALYGMHDRGTIEVAKKA
                     DFNVIDLDRLVLHRPEMHHDLPAGARRLLQRADGYVATIVNGEVIMQDGHETGARPGA
                     LVRSGRAA"
     gene            4467..>4898
                     /locus_tag="VIJ44_01920"
     CDS             4467..>4898
                     /locus_tag="VIJ44_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY5164686.1"
                     /translation="MSTNITDVDLANLDTFVAGVPHDMFDTLRRDAPLYFHPETNGAG
                     FWCVVRYHDLEQISRDPRMFSSEWGITLHESAEGELEQQRMMMLMMDPPRHTKQRLLV
                     NKGFTPRMIEQLHDRIRQAAVDIIDNIAERGECDFVVDVAAE"
BASE COUNT          754 a         1694 c         1684 g          766 t
ORIGIN      
        1 ctccggtaga tctgacccca acggacggtc cgtccgtcga ccgtcggata gccgccgagg
       61 tcccggaagt tcagcggccc cgccagggtc acgcgtcgat cgctcatggg ttcttacccg
      121 ccccgtgggc gagggtgtgc gccgcgaagc gtgcgtgatc cacgaggatg aagacgcctt
      181 cggcttcggc gcagagccgg tcaccggcat ggagggtgcc cacggttcgg accttgcggc
      241 cgtcgacgcc gagggtgcga gcggcgagcc ggagctcggt gtacagcggc gtcgggctcc
      301 ggtacacgac ggtcagcgtg ccggtcatgg cgccgacccc ggcgacgatg ttggccacgc
      361 ccagaagctc gtcgaagaga ccggcgatga tcccgccgtg gacgcagccg ggcggcccct
      421 cgtaggcggc gccgaggagc ccgtggcctc gcacgccacc ttcgtggcgc tcgcaatgta
      481 tgggcggggc gagcgggctg aacagcccga tcatggggct gaacgggaag aagtcgtgag
      541 gctcgagctc ggcgaggggt cgcgccggca ctgacggcgt ccagggtcgg gtcgcggcct
      601 cgaggagatc cgcggcccgc tcgacgagcg ccgcggcctc ggccagcatc accggtgacg
      661 cgccgggccg gacgacgagg tcggtgctgt gacgcaacgc ctcagtgagg cgcgccagct
      721 cggggccggg agggctcgag gcatcggggc catgcccgtc gacgatgttc ggctggccgt
      781 tcacaagcgc agactcagtt gagaagcgga ccggcgacta gtcctcggcg atcgagatcg
      841 cttgcttggg gcactggcgg acggcttgct cgaccttggg tcgcaagtcg tcaggcggct
      901 cctcttggag cacgtagagg aagtcgtcgt cacgaacctc gaagacttcg ggcgcgaccc
      961 ccatgcagat ggcattgctc tcgcagacgt cgaaatcgac tacgaccttc caggccatca
     1021 cgcctcctcc aggctcaacg cgggccgact ctactccggc ggtaggttca cgaagtccgg
     1081 ctcgcgcttc tccagaaagg cggcgatcgc ctcccggttc gcgggcgcgc cggtcagccg
     1141 ggcgaatacg tcctcctcgc cggctctcgc cgcttgggcc gctgagagcc atccggccct
     1201 catgagccgc ttcgtctcac tgagcgacac gatcggcagg cgagccatgg cccgggcggc
     1261 gtcgagcgct tcgtccagga gcagcgccgc gggacacgac ttccaggcca gccccgacgc
     1321 caccgcggtc gcggcgtcga accacgcgcc ggtcagcagg gcgtgggccg cgacctggcg
     1381 gcccatcgca accggaagcg tgaagctgct tcccgcctcc ggaacgacgc ccaagctggc
     1441 gaacggggcc cgaaaccggg cgtcgtcggc gacgagcacc agatcgcagt acggaagcat
     1501 cgtgacaccg aggccgaccg cgacgccgtt gaccgcggcg accaccggct tcgggaacga
     1561 ctcgagggcc ccgacgaacc gcgtgaaccc gtggccgttt cgcgcctcgt cggtggtcgc
     1621 gcttcgatca ccgagccgtc ccatctccgc caggtcctgt ccagcgcaga acgcgcccgc
     1681 cgagccggtg agggcgacga ccgccacgtc ggtccgggtt gcggcgtcgt caaaggcgtc
     1741 ggcgagcgcg tgatagtgga cagtgtccag agcgttccgc gcctcgggac ggtccagggt
     1801 taagagacgc acccggtccg cgtcgtcgac gtggagcact cagcgggccc cgaacgggtc
     1861 gatcgtcagg tgatggctga gatcgccgat gaggtcacag aagatcactc gctccgtcaa
     1921 ccgccactcg ccggccacgc gctcgaactg gtcgtgatac cgaccggcga tgatcgcctg
     1981 taacgggaag tcgtccacct gttggaggac agtgaagtac gagtgggcgg cggcggtgcc
     2041 ggcaccctcg tcggcgtcga cgacgaggtt cgtggtcacg tgcttggtgc ggggcgtccc
     2101 gtcgggatag cggcggacca tccgctcctg gatcgccagc accgcgttgg caccaacgac
     2161 accggggtcg tcggggccgc ctcctttgta ggtcgcgtgg cggaacagct ccgcgaggcc
     2221 ggcgaagtcg cccatgtcca ctcggtcggc gtagccgtag acgatccgct cgatcgcgct
     2281 gcgagcgtcg gtcaccgcca catcggcggc gtcaccggtc ggcgcccttc gccttcagcg
     2341 gctcggcgag ctgcagatcg aacccttcga tgtagtcgcc catcgacgca ccgagcttcg
     2401 cgaacgcatc gctgcccgcg tgcgcgtcga aggcaccctt gttggtgtag agctccgtca
     2461 tgtagaacac gttcgggttc gtcgtggagc ggtgcaggac gtactcctcg gtcccgtctt
     2521 cggtctcgac gtgacggaag aactcgtcga aggccgcggg gaactcgtcg gccttgccgt
     2581 ccttgacggt caacgtcgcg atcactgaca ccttgctcat cgtcgcctct cgctcaagcg
     2641 gcccggccgc tgcgcaccaa cgcgcccggc cgagccccgg tctcgtgtcc gtcctgcatg
     2701 atcacttcac cattcacgat cgttgccacg tacccgtcgg cgcgctggag tagtcgccga
     2761 gcgccggcgg gaaggtcgtg gtgcatctcc ggtcggtgca ggaccagccg atcgaggtcg
     2821 atgacgttga agtcggcctt cttggcaacc tcgatggtcc ctcggtcgtg catcccgtag
     2881 agcgccgcgg tgtcgctggt catcttgcgc acgaccagct cgagcgggag gcgctcaccc
     2941 cgggttcggt cgcggaccca gtgggtcagc atgtaggtct cgatgctggc gtcacagatc
     3001 gagccgacgt gggcaccgcc gtcgccgagc ccgagggcgc tcgtcgggtg catgaccatc
     3061 tcgcggatcg ggtcttgagt gaagttcgag tacccgagca gcgggcgcag cagcagctcg
     3121 cgaccatcgt tctcgaggag caggtcatag aacaggtccc atttgtcgcg gccgtcacgc
     3181 tcggcgatcg ccgcgatgct ctcctcgggc gcgggctcgt agtccggcgg ctcaccgagc
     3241 cggaagatcc ggtcgaggcc gagaccgatg taggcctcga ttccctcccg cttcggcgct
     3301 cgttcgctca ggatggctgc ccgcacgtcc ggttcccgaa ggcgggccac cttctcgtcg
     3361 aggtcgaggt gatcgagcgc ctggtaggtc gggcggtcgc gcagcgggtg gaaggtctgc
     3421 agcccgagga gcagcccgag cggccggccg gcgacctggg gccggaccgg taccccgtcg
     3481 tcgtacgcct cgagcgcgag gtcgagcatg cgcttccact ggtcgggatc gtggtcgtgt
     3541 tggagcagcg cgaaggtgac cggtcggctg gtctcgcgcg agagacgccg catccagtcc
     3601 atttcccggt cggacgccgc gaggtcctcc cccatcaccc cggcgggggc cagctcaaac
     3661 accccggacc ctgcttcgcc cagcgcgcgg cccatggcga acagctcttc ctcggcggcg
     3721 aacgtgccgg ggactggctc cccgtcgatc gcccgatggg cgatcgtccg cgacgtcgag
     3781 acgccaacgg cgccggcttc gacgccttcc cggaccagct tggccatctc ggcaatgtca
     3841 tcgggggtgg cgggctcgtt ccgggcaccg cgctccccca tcacgtagcc gcgcaccgcc
     3901 ccgtgcggga cttgggtcgc gacgtcgatg gccagcggct gcgcgtcgag gaagtcgagg
     3961 aactcgggga aggtctccca tccccagcgg atgcctgcgg acagggccga gccggggatg
     4021 tcctcgacgc cctccatcag gccgatgagc cagtcgcgtc ggtccggttt ggccggcgcg
     4081 aagccgaccc cgcagttgcc catgacgaca gtggtcacgc cgtgccatga cgacggggcc
     4141 agggtcggat cccatgtgac ctgaccgtcg taatgggtgt gaacgtcgac gaaacccggg
     4201 gtcaccacac agcccgtggc gtcgatgacc tcgtctccct cggcgtccac ccgtccgacc
     4261 gcggtgatgc gcccgtcgtc gacagccacg tcgcccggcg cggcatccgc cccggtaccg
     4321 tcgacgatgg tgccaccccg gatcacgagg tcgtgcatcg ctctccccct tgtcagtcgg
     4381 gcttcgagcg tcgtggaccg cccggtgcgc cagatcgtaa cgtgttccag tcagaccgcc
     4441 ttgcccgccc gacgaggagc agccctgtgt ccacgaacat caccgacgtc gacctggcga
     4501 acctcgacac gttcgtggcc ggcgtcccgc acgacatgtt cgacacgctg cgccgcgacg
     4561 cacccctcta cttccatccc gaaacgaacg gggcggggtt ctggtgcgtg gtgcgctacc
     4621 acgacctgga gcagatcagc cgggatcccc ggatgttctc gagcgagtgg ggcatcaccc
     4681 tccacgagtc cgccgagggc gagctcgaac agcagcgaat gatgatgctg atgatggacc
     4741 cgcctcgcca cacgaagcag cggctcctgg tcaacaaggg attcacgccc cgcatgatcg
     4801 agcagctcca cgaccggatc cgtcaggccg cggtcgacat catcgacaac atcgccgaac
     4861 gcggcgagtg cgacttcgtg gtcgacgtcg cggcggag
//
LOCUS       DATCCH010000095         2389 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_300770, whole genome shotgun sequence.
ACCESSION   DATCCH010000095 DATCCH010000000
VERSION     DATCCH010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300770"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="VIJ44_01925"
     CDS             complement(<1..220)
                     /locus_tag="VIJ44_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164687.1"
                     /translation="MSDGMVMAPVPNRAPEATDRSAAQKLVASVSAGIAKYSNLSAAV
                     ADGYVPATNPRGYVVHYANWEAVRAGDVL"
     gene            660..1403
                     /locus_tag="VIJ44_01930"
     CDS             660..1403
                     /locus_tag="VIJ44_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164688.1"
                     /translation="MTTRQIDFGPPAFEPVGEERGRKRAARWPVAFVVISSLAAACGG
                     TTPFATVVPTTRPVPTATPPAGRGLPAGYVPFVDAADHFSIAVPNTWRRIDLSTPGAS
                     QSLRDVVETNPGLKRVLGSNAAALIAQGIKFYAVNPKAGADVPAINVGVRPAVGIRDS
                     DLPHIGNTLRSQFATIGATISSISAASVAGHQALEVAVTFRAIGPTGAVTAKQEIQYF
                     IAANDFIYVLALAGTSPEFPAIAATFDVS"
     gene            1573..2226
                     /locus_tag="VIJ44_01935"
     CDS             1573..2226
                     /locus_tag="VIJ44_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164689.1"
                     /translation="MRGWPLFAAIAYVVLLFAGLVVVPAMPEVNASGARLVRYYHDHG
                     DGVRLATWLGTWSTVPIVLLVAHLRSRLTGMSRDVMMLGAAGLVVATVVWSWFNAGLA
                     LHPDTLDPRVARTVADVGAYFGPVITVSIILLIAPIGLAAWRREGGFPRWLAWLTVVF
                     AVEQSVETITVFGTRGFIAPGGPMNFTLGAGLFLVWVIGAGVAASSTRTAAVGLGVV"
BASE COUNT          358 a          771 c          824 g          436 t
ORIGIN      
        1 cgagcacgtc gccggcccga accgcctccc agttcgcata gtgcaccacg taacccctcg
       61 gattcgtcgc gggcacgtaa ccgtcggcga cggcagccga caggttcgag tacttggcga
      121 tcccggcgct cacggacgcc accagcttct gcgccgcgct tcgatcggtc gcttcggggg
      181 cacggttcgg gacgggcgcc atgaccatgc cgtcggacat caccgtcgca cccttcacgt
      241 cactacatac cgcgggcaca gccgccttgg tgttcgcggc atcggcgttc cgtgcgttgg
      301 tcgacggcgc cgccggggtg acagacgaat ttcgggcgcc gccaccgcga ccgctgtcgt
      361 tcgcgcctgc ccacgcgaac ccggccgcga ccagagcccc gacgatggcc acgaccactg
      421 ccgtgaaacc gaaccttccc cacctggcac gcgtctcttc cacgtgccca gtatcggcgg
      481 acgacgtgag gccggagcca cccggaacgt aagggaattg tgaacctaaa gaaatagtga
      541 ggtgccgcag ggccccgtat tcctgccggt ctcggggcca tcgcatcggg ccgatctgtt
      601 ccctgtaggg cgaggtcgac gatgccgata ggaggcggtc gggcgagctc ggaggatcgg
      661 tgacgacgcg acagatcgac ttcgggcccc cagcattcga gcctgtgggc gaagaacgcg
      721 ggagaaagcg tgccgcgcga tggcccgtcg cgttcgtggt gatctccagc ctcgccgccg
      781 cgtgtggagg caccacaccg ttcgccaccg tggtccccac gacccggccg gttccgacgg
      841 cgacacctcc ggcgggtcga ggtctgccgg ccggttacgt cccgttcgtc gacgccgcgg
      901 atcacttcag catcgccgtc ccgaacacct ggcggcggat cgatctgtcc actcccgggg
      961 cctcgcaatc gcttcgggat gtcgtggaaa ccaatcccgg cctgaagcgg gtcctgggat
     1021 cgaacgccgc ggcattgatc gcgcaaggca tcaagttcta cgccgtgaac ccgaaggccg
     1081 gtgccgacgt gcccgcgatc aacgtcgggg tacggcccgc ggtcgggatc cgcgacagcg
     1141 accttccgca tatcggcaac acgctgagga gtcaattcgc aaccatcgga gcgacgatct
     1201 cgagcatttc cgcagcctca gtggccggtc accaggcact cgaagtggca gtgacgtttc
     1261 gcgcgattgg tcctaccggc gccgtgaccg cgaagcagga gatccagtac ttcatcgcag
     1321 ccaacgactt catctacgtc ctggcgctcg ccggaacgag ccccgagttt cctgcgatcg
     1381 cggccacgtt cgacgtgtcg taggaaatcc gccgtcggta gcttcccgca ggagcgaaac
     1441 tgatcgacgt ttcaccgtcg atcggccgat cggtacgcgc tcaccgagcg cgggatcggc
     1501 cgatgtggtt ccctatggcc gatgtaattc cctgatggag tgtccgagcg ggttcgtcgg
     1561 cgggggtgcg ggatgagagg ttggccgctg ttcgcagcga tcgcgtacgt ggtgctgttg
     1621 ttcgcggggc tcgtcgtggt cccggcgatg ccggaggtga acgcgtcggg cgctcggctc
     1681 gttcgctact accacgacca tggcgacgga gtgcgtctgg cgacgtggtt gggcacgtgg
     1741 tcgacggttc cgatcgtatt gctcgtcgcg cacctgcggt cgcgtctgac cgggatgagc
     1801 cgagacgtga tgatgctcgg ggctgcgggg ttggtcgtcg cgaccgtcgt gtggtcgtgg
     1861 ttcaacgcgg gactcgcgtt gcatcccgac acactcgacc cgcgagtcgc tcggacggtt
     1921 gccgacgtgg gcgcgtactt cggtccggtc atcaccgtgt cgatcatctt gctcatcgct
     1981 ccgatcggtc ttgcggcgtg gcggcgtgag ggtggatttc cgcgctggct ggcctggctc
     2041 acagtcgtgt tcgccgtgga gcagtcggtc gagacgatca ccgtcttcgg gacccgaggc
     2101 ttcatcgcgc cgggcggacc gatgaatttc acgctcggtg ccggcctgtt cctcgtctgg
     2161 gtgatcggcg cgggtgtcgc agcttcgagc acacgaacgg ccgcggtcgg tcttggggtg
     2221 gtttgatgcg acgacacgtt ctcggcgcga cgagtgtgga gctgtccgcg gtcggtctcg
     2281 gcggcgtgtg gttgcgtgct gacgacgcga cgtcggccgg cggcgtgttg gcggcatcgt
     2341 tcgaggcggg agtgaattgg gtcgacaccg ccgagggata cggcgacgg
//
LOCUS       DATCCH010000096         2666 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_300955, whole genome shotgun sequence.
ACCESSION   DATCCH010000096 DATCCH010000000
VERSION     DATCCH010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300955"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="VIJ44_01940"
     CDS             <1..361
                     /locus_tag="VIJ44_01940"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HEY5164690.1"
                     /translation="TDEYDLPEPVVERLANGVLRVPGRTPIDEVNELLDVTLPQEEWD
                     TVGGLVFNTLGHVPIESECLRVDGLEFCAERVQGRRIVSVIITRLSDPCDDESPASDR
                     PNSDRPSSDRPSSDRGE"
     gene            358..1257
                     /gene="era"
                     /locus_tag="VIJ44_01945"
     CDS             358..1257
                     /gene="era"
                     /locus_tag="VIJ44_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007786750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEY5164691.1"
                     /translation="MSADFRSGFVSLVGRPNVGKSTLLNQIVGQKVSIVSDRPQTTRT
                     QVRGVRTTPNTQIVFLDTPGVHKPRTLLGERANGRALTTLGEVDVVCFLIEANAPIGA
                     GDRFVAARLAEVDTPVVLALNKVDVASRDQIVDHLAVASGELGDFAAYVPLSARAGDG
                     VDALLGELEARLPIGPHYYPEGVVSDQPESFLVAELLREQLLAMAREELPHSIAVTTD
                     EMEERETPDGPLLVVRVIVRVERDSQKGIVIGRGGAVLKAAGTAVRHELEALLGMRVH
                     LETRVKVDPDWQRRAGALDRLGL"
     gene            1297..2532
                     /gene="coxB"
                     /locus_tag="VIJ44_01950"
     CDS             1297..2532
                     /gene="coxB"
                     /locus_tag="VIJ44_01950"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEY5164692.1"
                     /translation="MRVTPPRAVGVPELSFGESTAVESGRSPAETRRRERLKLDRTHR
                     FRRAGTAAAIAIAVVTLDSCGDSTGPSNKQNALRPAGKYSRQILHLTEPFFWIAVVIG
                     LGVIGGTFFVALRFREKPGEERAVKQVHGNTILEISWTVIPAVILAIMAVPTVATIFS
                     LAKKPTGPNVVNVTVTARQWWWQFTYTDPGNGVETANELHIPVGAPISLTLQGPPACS
                     PAPCYLNGVIHSFWVPELNGKKDVVPGRKQFLKLEADKPGVYQGQCAEYCGLSHADMR
                     LRVIAQTPADYQAWVRSQLAQKTSEAALKAGVDSATWGCATCHSFKSGVPGAVAPNLT
                     HLADRTAFAGDKYLLNYDNLWKWVYDAPGRKPMGDLYQHMPAFNKAGMSQAEAQKIAC
                     FLLTNTATNPNPPSECAGK"
BASE COUNT          469 a          908 c          878 g          411 t
ORIGIN      
        1 aaccgacgag tacgaccttc ccgagccggt cgtcgagcgg ctggcgaacg gggtgctgcg
       61 cgttcccggc cggacgccga tcgacgaggt gaacgagttg ctcgacgtca ccttgccgca
      121 agaggaatgg gacacggtcg gcgggctcgt gttcaacacg ctcgggcacg tcccgataga
      181 gagcgaatgc ctgcgggtgg acggcttgga gttctgtgca gaacgcgtac agggccggcg
      241 gatcgtctcg gtgatcatca cgcgcctcag cgatccgtgc gacgacgaat ctccggcctc
      301 cgatcgcccc aactccgatc gacccagctc cgatcgaccc agctccgatc gaggcgagtg
      361 agcgccgact tccgctcggg cttcgtgtcg ctggtcgggc gtccgaacgt cggaaagtcg
      421 acgttgctca accagatcgt ggggcagaag gtctcgatcg tctccgaccg tccgcaaacg
      481 acgcgcaccc aggtgcgcgg cgtgcgaacc actccgaaca cccagatcgt gttcctcgac
      541 acgccggggg tgcacaagcc gcggaccctg ctcggtgagc gcgcgaacgg ccgcgcgctc
      601 acgacgctgg gcgaagtcga cgtcgtgtgc ttcctgatcg aggccaacgc cccgatcgga
      661 gccggcgacc ggttcgtcgc ggcccggctc gccgaggtcg acacacccgt cgtgctggca
      721 ctgaacaagg tcgacgtcgc gtcacgcgac cagatcgtcg accaccttgc ggtggcatcg
      781 ggtgagctcg gcgacttcgc cgcctacgtg ccgctttcgg cgcgtgccgg cgacggagtg
      841 gacgcgctgc tcggcgagct cgaggcgcgc ctgcccatcg gtccgcacta ttaccccgaa
      901 ggcgtggtct ccgaccagcc cgagtcgttc ctcgtggccg agttgctgcg cgagcagctc
      961 ctcgcgatgg cgcgcgaaga gctcccccat tccatcgcag tcaccaccga cgagatggaa
     1021 gaacgggaga ctcccgacgg accgctgctc gtcgtgcgcg tcatcgtgcg cgtcgagcgc
     1081 gattcgcaaa aggggatcgt catcggccgc ggtggggcgg tgctgaaggc ggcggggacg
     1141 gccgtccgcc acgagctgga agccctgctc gggatgcgtg tacacctcga gacgcgggtc
     1201 aaggtcgacc cggattggca gcgacgggcc ggggccctcg accggctcgg cctctgatcg
     1261 agcgcggggt ccaaccggcc cgttctgctt gtgatcatgc gcgtgacacc accccgcgcg
     1321 gtcggcgtgc cagagttgag tttcggagaa tcgaccgctg tagagtccgg ccgatctcct
     1381 gcagagactc gtcgccgaga gaggctgaag ttggaccgca cgcaccggtt ccggcgcgca
     1441 ggcacggctg ctgccatcgc gatcgccgtc gtcacgctcg acagctgtgg tgacagcacg
     1501 gggccgagca acaagcagaa cgcgctgcgt ccggccggga agtactcccg ccagatcctg
     1561 catctgaccg agccgttctt ctggatcgcg gtggtcatcg gcctgggtgt gatcggcggc
     1621 acgttcttcg tcgcgttgcg cttccgcgag aagccgggcg aagagcgcgc ggtgaagcag
     1681 gtgcacggca acacgatcct cgagatcagc tggaccgtca tccccgcggt catcctggcg
     1741 atcatggccg taccgaccgt cgccacgatc ttcagcctcg ccaagaagcc gaccggaccc
     1801 aacgtcgtga acgtcaccgt cacggcgcgg cagtggtggt ggcagttcac ctacaccgac
     1861 cccggcaacg gcgtcgagac cgcgaacgag ctgcacatcc cggtcggcgc gccgatctcg
     1921 ctgacgctgc agggtcctcc tgcgtgctcg cccgcgccgt gctacctgaa cggcgtgatc
     1981 cactcgttct gggtccccga gctcaacggc aagaaggacg tcgtccccgg ccgtaaacag
     2041 ttcctgaagc tcgaggccga caaaccgggc gtctaccagg gacagtgcgc cgagtactgc
     2101 gggttgtcgc acgccgacat gcgtctacgg gtgattgccc aaacgccggc cgactaccag
     2161 gcctgggtgc gcagccagct cgcccagaag acgtccgaag ccgcgttgaa ggcgggcgtc
     2221 gacagcgcga cctgggggtg cgccacgtgt cacagcttca agtccggcgt ccccggcgcc
     2281 gtcgccccga acctcacgca tctcgccgac cgcaccgcgt ttgccggcga caagtacctg
     2341 ctcaactacg acaacctgtg gaagtgggtg tacgacgcgc ccggtcgtaa gccgatgggc
     2401 gacctctacc agcacatgcc ggctttcaac aaagcgggga tgagccaagc ggaagcacag
     2461 aagatcgcgt gcttcctgct gaccaacacg gccacgaacc cgaacccgcc ttcggagtgt
     2521 gcaggcaaat gacaactgtc gaagaagcca ccacggtcgt tcacgcaccc gagcgacgcg
     2581 ttcgcatcct cgagcggccg cgagcgacga cgggcaagtt cagctgggtc accacggtcg
     2641 accacaagaa gatcgcgatc atgtac
//
LOCUS       DATCCH010000097         2106 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_259556, whole genome shotgun sequence.
ACCESSION   DATCCH010000097 DATCCH010000000
VERSION     DATCCH010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259556"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="VIJ44_01955"
     CDS             complement(<1..724)
                     /locus_tag="VIJ44_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164693.1"
                     /translation="MTVRHETSDPEVTEPLPIPESLLVPMLDAAAAVLRDLDPGDFPP
                     AVRPLASFGKQGLTTSPARQQLRRALDVDEHFRARAATRFLARPAVVAVLETWDAAHA
                     LRRVDDAAERSDLPLLASALYAARPEGWAFGIGVVCASFDRKRVEKEAEDDAKARQLQ
                     LSTLDEARRRAEEDRERSRHDVARLEEQLRAERRSRRDRDHRAEQGIEAAERTRTEAE
                     ALVAKARAATEEAEARLEREATR"
     gene            complement(729..968)
                     /locus_tag="VIJ44_01960"
     CDS             complement(729..968)
                     /locus_tag="VIJ44_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017573862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164694.1"
                     /translation="MGDPVRYRCAGCGNVTRFDVVAIRRTRSFHHYSLAGELTVEDES
                     VLDEQIVEVTCRWCGASGDTVVEVAEPSAEATSSG"
     gene            complement(1040..1465)
                     /locus_tag="VIJ44_01965"
     CDS             complement(1040..1465)
                     /locus_tag="VIJ44_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164695.1"
                     /translation="MSPAGRGPAVPPAAPGRGAGKPPRDALPLTVRVGAWVLAVPIGL
                     AIVGVPARILGYLTSQKLLDVIVKHNLSRFVPLLVIVVLWALATTLLVEVFIEGGRWL
                     MLRRRRRSGGPGGADDFDAGPVPPRPRSRSRRPAGTRRS"
     gene            complement(1770..>2106)
                     /gene="trpD"
                     /locus_tag="VIJ44_01970"
     CDS             complement(1770..>2106)
                     /gene="trpD"
                     /locus_tag="VIJ44_01970"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013269553.1"
                     /note="Catalyzes the conversion of
                     N-(5-phospho-D-ribosyl)-anthranilate and diphosphate to
                     anthranilate and 5-phospho-alpha-D-ribose 1-diphosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEY5164696.1"
                     /translation="STIHELREGTISEFEIDPTVLGLPLVDRAALAGGDADLNAQVVH
                     AVLDGKPGPNRDIALLNAAAALVVAGVADDLPDGLEAARASVDDGRARAALDAFVRTS
                     VAARDAEAA"
BASE COUNT          293 a          742 c          796 g          275 t
ORIGIN      
        1 cccgggtcgc ttcgcgttca agacgggcct cggcctcctc ggtggcggcg cgagccttcg
       61 cgaccaaggc ttcggcctcg gtgcgggttc gctccgcggc ctcgatgccc tgctcggctc
      121 ggtgatcacg gtcgcgacgc gatcgacgtt cggcgcgcag ctgctcctcg aggcgggcga
      181 cgtcgtgtcg ggatcgctcg cggtcctcct cggctctccg gcgcgcttcg tcgagcgtgg
      241 acagctggag ttgacgggcc ttggcgtcgt cctcggcttc tttttcgacc cgcttgcggt
      301 caaaggacgc gcagaccacg ccgatcccga acgcccagcc ttcgggtcgg gcggcataca
      361 gcgccgaggc cagtagcggc aggtccgagc gctccgcggc gtcgtcgacg cggcggaggg
      421 cgtgggccgc gtcccaggtc tcaagcaccg cgaccaccgc ggggcgggcg aggaagcgcg
      481 tcgcggcccg ggcccggaag tgctcatcga cgtcgagcgc ccgccgcagt tgctgacggg
      541 cgggactcgt cgtcaaccct tgcttcccga agctcgccag cggccgaacc gcaggcggga
      601 agtcgccggg gtcaaggtcc cggaggaccg cggcggcggc gtcgagcatc gggacgagca
      661 acgattcggg tatcggcagc ggctcggtga cctcggggtc cgacgtctcg tgtcggacgg
      721 tcacgtcgtc atcccgagct cgtggcctcg gctgatggtt ccgcgacctc gacaacggtg
      781 tcaccgctgg cgccacacca gcgacaggtc acctcgacga tctgctcgtc gaggaccgac
      841 tcgtcttcga cggtgagctc acccgcgagg ctgtagtggt ggaaggaccg ggtccgccgg
      901 atcgcgacga cgtcgaagcg agtgacattc ccgcaaccgg cgcagcggta acggaccggg
      961 tcgcccattt cggcagtgta cccggcaggt cgcggatatc gaacgtacgt tccgaccctg
     1021 agggggcgtc ggtacccggc tacgagcggc gcgtgccggc ggggcgacgc gaacgcgacc
     1081 tcggtcgggg cggtaccgga ccggcgtcga agtcgtcggc tccccccggg ccacccgagc
     1141 ggcgccgccg gcgcagcatc agccaccggc cgccttcgat gaagacctcg acgagcaagg
     1201 tggtggcgag cgcccacaac acgacgatga cgagcagggg aacgaagcgc gacaggttgt
     1261 gcttcacgat gacgtcgagg agcttctggc tcgtgaggta gccgaggatc cgagcgggca
     1321 cgccgacgat cgcgagcccg atcggcaccg ccagcaccca ggcgccgacc cggaccgtga
     1381 gcgggagggc gtcgcggggc ggcttccccg cgccgcggcc cggcgcggca ggtggaacgg
     1441 ccggtccacg gccggcgggg gacaccaccg tcccgcccgg cggtcccgag ctgggggtgg
     1501 cgacgacagg ggccgcggcc ggtgccggcc gcggcggacg cgcggtgggt gccggtgacg
     1561 actcgggagg agcggcgcgg cgccgcgggg gcggcgcggc gccaccaccc cgagggggag
     1621 gagacgacga gccgccgccc cgcgcggggc gagccaccgg cgggaccttc agcacttgcc
     1681 cgcaattctc acagcggaac gtgtcggttg cgagcccgtc gagacgcttc ttcgtgccac
     1741 agctcggaca ctggagggcc tgggccatct caggccgcct cggcgtcgcg agccgcgacg
     1801 ctggtgcgca cgaaggcgtc gagcgccgcc cgcgcccggc cgtcgtccac ggacgcccgt
     1861 gccgcctcga gcccgtcggg gaggtcatcc gcgacgccgg cgacgacgag cgcggccgcg
     1921 gcattgagca acgcgatgtc ccggttcggg cccggcttgc cgtccagcac cgcgtgcacc
     1981 acctgcgcgt tcaggtcggc gtcaccaccg gcgagggcgg cgcggtcgac gagcgggaga
     2041 cccagcaccg tggggtcgat ctcgaactcg gagatcgtcc cctcgcgaag ctcatggatt
     2101 gtggac
//
LOCUS       DATCCH010000098         2253 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_259585, whole genome shotgun sequence.
ACCESSION   DATCCH010000098 DATCCH010000000
VERSION     DATCCH010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259585"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VIJ44_01975"
     CDS             <1..288
                     /locus_tag="VIJ44_01975"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY5164697.1"
                     /translation="EPFPTFAGRVRAALEETTGQLGSGETALLCTSSGVISAACTTLL
                     GLPDDAFLAFNRVTVNAGITTIALGRRGATLVSFNEHGHLLAGGRSLLSYR"
     gene            complement(292..2196)
                     /locus_tag="VIJ44_01980"
     CDS             complement(292..2196)
                     /locus_tag="VIJ44_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017206871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HEY5164698.1"
                     /translation="MSSHPLDPLTADEVRLAVAIVRREQDVSDRWRFASIELREPSKA
                     VLTDFAPGTAFARAARVVCWNRDDGQTHTAVVSLSEGRVTSWLHQPDGQPNMTADEFH
                     ECDAAMRAEPRVIEALARRGIEDMDRVLIEAWAYGAHLVPERYTSRRLGWTDVWYRKQ
                     EGSSPYANHVSGLHFIVDLNRMELLEVEDHGAVEEPQTMGEYIPRLVPGQRLRSDVKP
                     LEIVQPEGASFSLEGNLLRWQKWSLRVGFNYREGLVLHTVGYEDGGRLRSVAHRLSFA
                     EMVVPYRDPTPEHYRRTAFDIGEWGLGFMTTSLELGCDCLGEISYLDAVLHDTTGEPY
                     TIRNAICIHEEDDGVLWKHVDSETGAETRRQRRLSVSFHATVGNYEYLVYWRFYQDGN
                     IQVEVRATGIMVTTRLPEGDHPRNGTIVDERTYAPFHQHFLVARLDLDIDGEDNTVHV
                     TDSEASPTGPDNPYGLALVQRSTPLRTEDAGKQDYDWSTQRAWKVVNERVPNGLGAPV
                     GYKLVPGDCFPAMFDAASPVLQRAQVLAHTLWVTPFDEDERWPCGEFVVQSREDRGLP
                     VWTGQNRSIEDTDVVLWYVFGIHHITRPEEWPIMSADTVSFWLKPVGFFDRNPALDVP
                     PTPHHRPRPA"
BASE COUNT          368 a          780 c          739 g          366 t
ORIGIN      
        1 gagccattcc cgacgttcgc cggccgggtc cgggcggcgc tcgaggagac caccggacag
       61 ctcggctcgg gagagacggc gctcctgtgc acgtcgagcg gggtcatcag cgccgcctgc
      121 acgaccctgc tcgggctgcc cgacgatgcg tttctcgcct tcaaccgcgt caccgtcaac
      181 gccgggatca ccaccatcgc cctcgggcgc cgtggcgcga cgctcgtctc cttcaacgag
      241 cacggccacc tgctggccgg cgggcggtcg ctgctcagct accgctgagg ctcacgctgg
      301 ccggggacgg tgatggggcg tgggcggcac gtcgagtgcc gggttgcggt caaagaagcc
      361 aaccggcttg agccagaagg acaccgtgtc cgcggacatg atcggccact cctcgggccg
      421 agtgatgtgg tgaatgccga agacgtacca cagcacgaca tcggtgtcct cgatcgaccg
      481 gttctgcccg gtccagaccg gcagtccccg atcctcccgg ctctggacga cgaactcgcc
      541 gcacggccac cgctcgtcct cgtcaaacgg tgtgacccag agggtgtgag cgagcacctg
      601 ggcgcgctga agcaccggcg aggcggcgtc gaacatcgcc ggaaaacagt cacccgggac
      661 gagcttgtag ccgacagggg cgcccagccc attgggaacg cgctcgttga cgaccttcca
      721 tgcgcgctga gtgctccagt catagtcctg cttgccggcg tcctcggtgc gtagcggagt
      781 gctccgctgg acgagcgcca gcccgtaggg gttgtccggc ccggtcggcg acgcctcgga
      841 gtcggtcacg tggacggtgt tgtcctctcc gtcgatgtcc agatccagcc gcgcgacgag
      901 gaagtgctgg tggaacggcg cgtaggtccg ctcgtcgacg atggtcccgt tgcgcggatg
      961 gtcgccctcc ggaaggcgag tggtcaccat gatcccggtc gcgcgcacct caacctggat
     1021 atttccgtcc tggtagaagc gccagtacac gaggtactcg tagttgccga cggtggcgtg
     1081 aaacgacacc gacagccgcc gttgccggcg agtctcggct ccggtctcgg agtcgacgtg
     1141 cttccacaga acgccgtcat cctcctcgtg gatgcagatc gcattgcgga tcgtgtatgg
     1201 ctccccggtt gtgtcgtgca gcacggcgtc aaggtaggag atctcgccca ggcagtcaca
     1261 accgagctcc aatgacgtcg tcatgaagcc gagcccccac tccccgatgt caaacgcggt
     1321 gcgtcggtag tgctcaggtg tgggatcccg gtaggggacg accatctcgg cgaacgacag
     1381 gcggtgtgcc accgaacgga gacggccgcc atcctcgtag cccaccgtgt ggagcacgag
     1441 tccctcgcgg tagttgaagc cgactcgcag cgaccacttc tgccagcgca gcagattccc
     1501 ctccaggctg aacgacgcgc cctcgggctg tacgatctcg agcggcttga cgtccgagcg
     1561 cagtcgctga ccggggacca gccgtgggat gtactcgccc atcgtctgcg gctcctcgac
     1621 ggcgccgtgg tcctcgacct ccagcagctc catccggttg agatcgacga tgaaatgcaa
     1681 gcccgacacg tggtttgcgt acgggctcga gccctcctgc ttgcggtacc agacgtcggt
     1741 ccaccccagg cggcgactcg tgtaacgctc gggcacgagg tgggctccat acgcccaggc
     1801 ctcgatcagc acccggtcca tgtcctcaat cccgcggcga gccagcgcct cgatcacgcg
     1861 cggctctgct cgcatcgcgg cgtcacactc gtgaaactcg tccgccgtca tgttcggctg
     1921 cccgtcgggc tgatggagcc agctcgtgac gcgaccttcg gagagcgaga ccaccgccgt
     1981 gtgggtctga ccgtcatcgc ggttccagca caccacccgg gccgcccgcg cgaacgccgt
     2041 cccgggtgca aagtcggtga gcaccgcctt ggacggctcg cgcaactcga tcgaggcgaa
     2101 acgccaccga tcgctcacgt cctgctcgcg gcgcacgatc gccacggcca aacggacctc
     2161 atcagcggtg agcggatcca gcgggtggct tgacatgacg cgggatggtc gagcatcacc
     2221 gcccgcgacg caacccgggc gccgctgacg ccg
//
LOCUS       DATCCH010000099         7140 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_467729, whole genome shotgun sequence.
ACCESSION   DATCCH010000099 DATCCH010000000
VERSION     DATCCH010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7140
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467729"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..127)
                     /locus_tag="VIJ44_01985"
     CDS             complement(<1..127)
                     /locus_tag="VIJ44_01985"
                     /inference="COORDINATES: protein motif:HMM:NF037181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164699.1"
                     /translation="MSHRRVTLRAVTTRPAHRCTACGAEAPRWLGRCPDCGTWGSF"
     gene            179..799
                     /locus_tag="VIJ44_01990"
     CDS             179..799
                     /locus_tag="VIJ44_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018490518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEY5164700.1"
                     /translation="MPDRSPLVVGFDLDMTLVDSRPGIATTLAALAAETGTPIDADVV
                     VGRLGPPLEDEMAHWFPTDEVASVSDRFRELYADLGVPGTVLLDGAAESVAAVRSVGG
                     GVVVVTAKFESNAVACLDLVGLAVDAVCGWRHGPDKGVALAEHGAAVYVGDTPTDVEG
                     ARGVGIVAVGVATGPHPAGELHAAGADMVLGSLTEFPAWLRAWLTG"
     gene            complement(756..1613)
                     /locus_tag="VIJ44_01995"
     CDS             complement(756..1613)
                     /locus_tag="VIJ44_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164701.1"
                     /translation="MTSGPSGGPARPRVWAPIAAVALGIILGGCSARATVRVSMRRDG
                     SGTVQAAVALDADAVQAAEAGGATLEQRVRLADLHAAGWTVSPWVASRNGGATLTVTK
                     RFQSPGQVAGIARELSGTTGPLRNFVAARGAAWVGLGHRATLRGTVDLSAAQPGVTTD
                     QQLVASLAGQHIDVNAINRQLLAQLRSSVSVRVVADLPGSRQTITVQAGKRQQLDAAA
                     TTFDTARVALLGVAVALAFLAAVVWPRGGRRRAPRRHPGQHQRPGRRRRSGRRVSPSA
                     TPATRPGTR"
     gene            complement(1660..4170)
                     /locus_tag="VIJ44_02000"
     CDS             complement(1660..4170)
                     /locus_tag="VIJ44_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020549609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEY5164702.1"
                     /translation="MFERFTDRARRVVVLAQEEARLLNHNYIGTEHILLGLIHEGEGV
                     AAKALESLGISLEAVRAQVEEIIGHGGQAPSGHIPFTPRAKKVLELSLREALQLGHNY
                     IGTEHILLGLIREGEGVAAQVLVKLGADLSRVRQQVIQLLSGYAGAKEGQGQGGAGGE
                     SQPSGSLVLDQFGRNLTQLAREKKLDPVIGREKEIERVMQVLSRRTKNNPVLIGEPGV
                     GKTAIVEGLASDIVGGDVPETIKGKQLYTLDLGALVAGSRYRGDFEERLKKVLKEIRT
                     RGDIILFIDELHTLVGAGAAEGAIDAASILKPMLARGELQTIGATTLDEYRKHLEKDA
                     ALERRFQPIKVEEPTVTQTIEILKGLRDRYEAHHRVTITDQAVVAAANLADRYISDRH
                     LPDKAIDLIDEAGSRLRIRRMQAPPDFRELEDEIATVRKDKEEAIEGQQFERAASLRD
                     REKELLEQRRTKETEAQREGVDLFNEVSEESIAEVLALWTGIPVYKLTEEETAKLLRM
                     EDELHKRVIGQDSAIRAVSQAIRRTRAGLKDPKRPSGSLIFLGPSGVGKTETAKTLAE
                     FLFGDEAALIQLDMSEYMEKHTVSRLVGSPPGYVGYDEGGQLTEAVRRKPFSVVLFDE
                     IEKAHPDVFNTLLQILEDGRLTDAQGRTVDFKNTVIIMTSNLGTADLRKASIGFGPAD
                     EAVTYEKMKDKVVEELKRHFRPEFLNRIDEVIVFAELSQEEVGEIVHLMIRRVQDQLE
                     SQGLGLELTPEAKLLLARRGYDPQLGARPLRRAIQRLVEDPLSEKILWKEFHAGETIV
                     VDADGEEVTFRAVAGVEPPPIEGVEPPPVELAGSGPEG"
     gene            4407..5384
                     /locus_tag="VIJ44_02005"
     CDS             4407..5384
                     /locus_tag="VIJ44_02005"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEY5164703.1"
                     /translation="MSLAETLELPPLPGDLKRVERALAGAVEHQDRFLGDVAGHLVAA
                     GGKRIRPTLTLCAAYAALGSDRPASDDAVTGAVAVELVHLGSLYHDDVIDEAETRRGV
                     PSVNARWSNIVAILAGDYLLARASGLAASLGAPVAGLLAATIGELCRGQVLELQHLFD
                     VGRSEEDYATAIEGKTASLFATACRIGGMVSDVDEPTLDALTRFGTHLGLCFQIVDDC
                     LDLTATDATLGKPAGQDLVEGIYTLPVIYALADDPGLSDRLGGPLEGERLAEVRVRAA
                     ANGAVDAALAVARDHATKAGEALTGADTLDAEVCKSLGHLVDGLVTRQS"
     gene            complement(5401..6225)
                     /locus_tag="VIJ44_02010"
     CDS             complement(5401..6225)
                     /locus_tag="VIJ44_02010"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY5164704.1"
                     /translation="MSVATARDGVPITYQTFGRPDAAPLLMLQGLGVDSRGWALQRFP
                     LGRRFRCVAVDNRGVGGSARAPHPLSLEQMADDAMAVLDHAGVDDAHVMGASMGGVLA
                     QIVAVRHPERVRSLVLSCTACRHHEWRRELFEDWSAAVLAGGMGALGGEGLRWLIGPR
                     LHRRVGLWLNVMARILQQAPPENFAAQVQAILAASDELRFELVNVHAPTLVITGSQDL
                     LTPLGDADELAELIPTARLLEVRGVGHALMVEAPNAFNRAVAGFLREVDAATVSVA"
     gene            complement(6222..6896)
                     /locus_tag="VIJ44_02015"
     CDS             complement(6222..6896)
                     /locus_tag="VIJ44_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164705.1"
                     /translation="MTVPVPNYGLDTDRVLATDSLPAALRRRLRGQYAVDEFGADPHL
                     MDLTAGLVALPVTVEHGEHVPQVGPALLVANRGLGFLEPLAVVRAVHVACGRRARVVG
                     APEVPVLGDALRKLGAIGYRPDDVAALLRAGNVAAAPLASTWFRSGPGEPPRALLVAT
                     LGFPVLPVAVQPGFPFGRPGRPWRVRFGEPLFPPAGTGPDDQLAAAELAAEVQTRVQA
                     LLEDRA"
     gene            complement(6893..>7140)
                     /locus_tag="VIJ44_02020"
     CDS             complement(6893..>7140)
                     /locus_tag="VIJ44_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164706.1"
                     /translation="GGRIPIPVVGFGWELAAEAAELAGAPIPPHVVELLRHGRCGDGA
                     RAVAELDLGSLRPTQEVLVDLFEWATVLRLEPTRRVA"
BASE COUNT          939 a         2306 c         2778 g         1117 t
ORIGIN      
        1 cgaaggatcc ccaggtgccg cagtcgggac agcggccgag ccagcggggc gcctcggcgc
       61 cgcaggcggt gcagcggtgc gcgggccggg ttgtcacggc ccgcagcgtc acacgccggt
      121 gtgacaccgg gccggcaaat cggcccgggc cgcgacgggc ggggccacac tgtcggccgt
      181 gccggaccgc tctccgctcg tggtgggttt cgacctcgac atgacgctgg tcgattcgcg
      241 gcccgggatc gcgacgacgc tcgccgcgct cgccgcggag accgggaccc cgatcgacgc
      301 cgacgtggtg gtgggccgac tcggtccgcc gctcgaggac gagatggcgc actggttccc
      361 gaccgatgag gtcgcgagcg tgtcggaccg gttccgtgag ctctacgccg acctcggcgt
      421 tcccggcacg gtcttgctcg acggcgcggc ggagtccgtg gccgcggtcc ggtcggtggg
      481 tggtggggtc gtggtggtca ccgccaagtt cgaatcgaac gcggtggctt gcctcgacct
      541 cgtcggtctg gcggtggatg cggtgtgcgg atggcggcac gggcccgaca agggggtggc
      601 cctcgccgag cacggcgccg cggtgtatgt cggcgacacc ccgaccgacg tcgaaggtgc
      661 gcggggagtg gggatcgtgg cggtgggcgt cgccaccggt ccgcatcccg ccggtgagct
      721 ccacgcggcc ggtgcggaca tggtgttggg ctcgctcacc gagttcccgg cctggttgcg
      781 ggcgtggctg acgggctgac gcgtcggccc gatcgtcgtc tccgtccggg acgttggtgc
      841 tggcccgggt ggcgtctcgg ggcgcggcgc cggccgcccc gcggccacac gacggcggcg
      901 aggaacgcca acgccaccgc gacgccgagg agcgcgaccc gggcggtgtc gaaggtggtc
      961 gcggcggcgt cgagctgctg gcgcttcccg gcctggacgg tgatggtctg gcggctgccg
     1021 gggaggtcgg cgaccacccg aaccgacacc gagctgcgca gctgggcgag gagctggcgg
     1081 ttgatggcgt tgacgtcgat gtgctgcccg gcgagactgg ccacgagttg ctggtcggtg
     1141 gtcaccccgg gttgcgcggc gctcaggtcc acggtcccgc ggagcgtggc gcggtgcccg
     1201 agaccgaccc acgccgcgcc gcgagccgcg acgaagttgc gcagcggccc ggtggtcccg
     1261 ctgagctccc gggcgatgcc cgccacctgg cctggcgact ggaaccgctt ggtgacggtg
     1321 agggtggcgc cgccgttcct gctggcaacc cagggggaga cggtccatcc ggcggcgtgc
     1381 aggtcggcga gtcggacccg ttgctcgagg gtcgcgccgc ccgcttcggc ggcttggacc
     1441 gcgtcggcgt cgagcgcaac tgcggcctgc acggtcccgg aaccgtcgcg ccgcatcgag
     1501 acccgcacgg tcgcccgggc cgagcagccg ccgaggatga tcccgagtgc gacggcggcg
     1561 atcggcgccc agacccgcgg tcgagccggt ccgcccgacg ggcctgacgt cacactgctc
     1621 gaggcttcgt accggcgccg cgccgaggga tgggttcgtt cagccttcgg ggcccgagcc
     1681 ggcgagctcg accggtgggg gctcgacacc ctcgatcggt gggggctcga cacccgcgac
     1741 cgcccggaag gtgacctctt cgccgtcggc gtcgacgacg atcgtctcgc cggcgtggaa
     1801 ctccttccag aggatcttct cggagagggg gtcctcgacg aggcgttgga tggctcgccg
     1861 caacggacgg gcaccgagct gagggtcgta gccccgacgg gcgagcagca gcttcgcttc
     1921 gggggtgagc tccaacccga gaccctggct ttcgagttgg tcctgcacgc ggcggatcat
     1981 gaggtggacg atttcgccga cctcctcttg ggacagctcg gcgaagacga tgacctcgtc
     2041 gatgcggttg aggaactcgg ggcggaagtg ccgcttgagc tcctcgacca ccttgtcctt
     2101 catcttctcg taggtgacgg cctcgtcggc cggcccgaac ccgatggatg ctttgcgcag
     2161 gtcggcggtc ccgaggttcg aggtcatgat gatcacggtg ttcttgaagt cgaccgtgcg
     2221 accttgggcg tcggtgagtc gcccgtcttc gaggatctgc agcaaggtgt tgaagacgtc
     2281 ggggtgggct ttctcgatct cgtcgaagag caccaccgag aacggcttgc ggcgcaccgc
     2341 ttcggtgagc tggccgcctt cgtcgtagcc gacgtagccg ggcggggagc ccacgaggcg
     2401 cgacacggtg tgcttctcca tgtactcgct catgtcgagc tggatcagcg ccgcttcgtc
     2461 accgaagagg aactcggcga gcgtcttggc ggtctcggtc ttcccgaccc cggagggtcc
     2521 gaggaagatg agcgacccgg aaggccgctt cgggtccttg agaccggcgc gggtgcgtcg
     2581 aatggcctgc gacaccgccc tgatggcgct gtcctggccg atgacccgct tgtggagctc
     2641 gtcttccatg cggaggagct tggcggtctc ctcctcggtg agcttgtaca cggggatgcc
     2701 ggtccagagc gcgaggacct ccgcgatcga ttcttcgctg acctcgttga agaggtcgac
     2761 accttcgcgt tgggcttcgg tctctttggt gcggcgctgc tcgagcagct ccttctcacg
     2821 gtcgcggagc gacgcggccc gttcgaactg ctggccttcg atcgcttcct ctttgtcctt
     2881 gcggacggtg gcgatctcgt cctcgagctc ccggaagtcg ggcggagcct gcatgcggcg
     2941 gatccgcaac cgcgaaccgg cttcgtcgat gaggtcgatc gccttgtcgg ggaggtgacg
     3001 gtccgagatg tagcggtcgg cgaggttcgc ggccgcgacg accgcctggt cggtgatcgt
     3061 gacgcggtgg tgggcctcgt accggtcgcg cagacccttg aggatctcga tggtctgggt
     3121 gacggtgggc tcttcgacct tgatgggctg gaagcggcgt tcgagcgcgg cgtctttctc
     3181 gaggtgcttg cggtactcgt cgagggtggt cgcaccgatg gtctggagct cgccgcgggc
     3241 gagcatgggc ttgaggatgc tggccgcgtc gatggcgcct tcggcggcgc cggcaccgac
     3301 gagggtgtgg agctcgtcga tgaagaggat gatgtcaccg cgggtgcgga tctccttgag
     3361 gaccttcttc agtcgctctt cgaagtcacc gcggtagcgg gaaccggcga cgagcgcgcc
     3421 gaggtcgagg gtgtagagct gcttgccctt gatggtctcg gggacgtcac cgccgacgat
     3481 gtcggacgcc agcccttcga cgatggcggt tttgccgacg ccgggttcac cgatcaggac
     3541 cgggttgttc ttggtgcggc gggacaggac ctgcatgacc cgctcgatct ccttctcgcg
     3601 cccgatgacc gggtcgagct tcttctcccg ggcgagctgg gtgaggttgc ggccgaactg
     3661 gtcgaggacg agggaccccg acggctggga ttcgccgccg gcgccgcctt ggccttggcc
     3721 ttctttggcg ccggcgtatc cggagaggag ctggatgacc tgctggcgga cccgggacag
     3781 gtccgcgccg agcttgacaa ggacttgggc cgcgacgcct tcgccttcgc ggatgagacc
     3841 gaggaggatg tgctcggtgc cgatgtagtt gtggccgagt tggagcgctt cgcgaagcga
     3901 cagctcgagc accttcttgg cccggggtgt gaaggggatg tggccggagg gggcctggcc
     3961 gccgtggccg atgatctctt cgacttgggc ccggaccgct tcgaggctga tgccgagcga
     4021 ctcgagggct ttggccgcga cgccctcacc ttcgtggatc agcccgagga ggatgtgctc
     4081 ggtgccgatg tagttgtggt tgagcaggcg cgcctcttct tgcgccagca cgaccacccg
     4141 tcgagcccgg tcggtgaagc gttcgaacac ggtgcctacc gtcgtctttc tggtcgcccg
     4201 tcccacgtcg ccatgagcgt gggaccggac caccgtcgtt gtgttccagt ctacccgtgc
     4261 gggtccgcgc gccttctgcc agaccgggcc ctcacccttg aggttctcgg ctgtttggag
     4321 gccgcgcgtg agcggtacca agtgccagaa gcgcgaaatt gttcccggct tggtgggacc
     4381 cgccggggct gcgtagcgtg ggccgtatga gcctggcgga gacgctcgag ctcccgccgc
     4441 tgccgggcga cctcaagcgg gtggagaggg cgctggcggg cgcggtcgaa caccaggacc
     4501 ggttcttggg tgacgtcgcg ggtcatctcg tcgcggccgg tgggaagcgg atccgcccga
     4561 cgttgacgtt gtgcgccgcc tacgccgccc tcggctcgga ccggccggcg tcggacgacg
     4621 cggtgaccgg ggccgtggcc gtggagctgg tgcatctggg gtcgctgtac cacgacgatg
     4681 tgatcgacga ggccgagacc cgccgggggg tcccgtcggt gaacgcccgc tggtcgaaca
     4741 tcgtggcgat cctggccggg gactacctgc tggcccgggc gtcggggttg gcggcgtcgc
     4801 tgggggcgcc ggtggcgggc ctgctggcgg cgacgatcgg ggagttgtgc cggggccagg
     4861 tgctggagct gcagcacctg ttcgatgtcg gccggtccga ggaggactac gccaccgcga
     4921 tcgagggcaa gaccgcgtcg ctgttcgcca cggcgtgtcg catcgggggg atggtgagcg
     4981 acgtcgacga gccgaccttg gatgccttga cccggttcgg cacccatctc ggcttgtgct
     5041 tccagatcgt ggatgactgc ttggacctga ccgcgaccga tgccacgctg ggcaagccgg
     5101 cgggtcagga cctggtggag ggcatctaca cgctgccggt catctacgcg ctggccgacg
     5161 accccgggct gagcgaccgg ctcggtggcc cgctcgaggg ggagcgtctg gccgaggttc
     5221 gggtccgagc cgccgcgaac ggcgccgtcg acgccgcgct ggccgtggcc cgcgaccatg
     5281 cgacaaaggc cggggaggcc ttgaccggcg ccgacacgct cgacgccgag gtgtgcaagt
     5341 cgttgggcca cctcgtcgac gggctcgtga cgcgccagag ctgagcgtcg atcgtcgcgt
     5401 ttaggcgacg ctcacggttg cggcgtcaac ctcgcggaga aacccggcga cggcccggtt
     5461 gaacgcgttc ggggcttcga ccatgagcgc gtgcccgacg ccgcgcacct cgaggagccg
     5521 ggccgtgggg atcagctcgg cgagctcgtc ggcgtcgccg agtggcgtga gcaggtcttg
     5581 ggagccggtg atcaccagcg tgggcgcgtg cacgttcacc agctcgaagc gcagctcgtc
     5641 ggacgcggca aggatggcct ggacctgcgc ggcgaagttc tcgggtgggg cctgctgcag
     5701 gatccgcgcc atgacgttga gccacagccc gacccggcgg tgcaggcgcg ggccgataag
     5761 ccatcggagc ccctcgccgc cgagcgcgcc catgccgccg gcgagaaccg cggcggacca
     5821 gtcctcgaac agctcgcggc gccactcgtg atggcgacac gcggtgcacg agagcacgag
     5881 ggagcggacc cgttcgggat ggcggaccgc gacgatctgg gcgaggaccc cgcccatcga
     5941 ggcgcccatc acgtgggcgt cgtcaacccc ggcgtggtcg aggacggcca tggcgtcgtc
     6001 ggccatctgc tcgagcgaga gcggatgagg ggcgcgggcg ctgccgccga cgccccggtt
     6061 gtcgaccgcg acgcagcgga agcggcgacc gagcgggaag cgctgcaacg cccagccgcg
     6121 cgagtcgaca ccgaggcctt gcagcatgag caacggggcg gcgtcggggc gcccgaaggt
     6181 ctggtacgtg atggggacgc cgtcgcgggc ggtcgcgacg ctcatgctcg gtcctcgagg
     6241 agggcctgga cgcgggtctg gacctcggcc gcaagctccg cggccgcgag ctggtcgtcg
     6301 gggccggtcc cggccggtgg gaacagcggc tccccgaagc ggacccgcca cggccggccg
     6361 ggccgaccga acgggaagcc gggttggacc gcgacgggca gcacggggaa cccgagggtg
     6421 gcgacgagca gcgcccgggg cggctcaccg ggaccggacc ggaaccacgt cgaggcgagt
     6481 ggcgcggcgg cgacgttgcc ggctcggagg agcgcggcga cgtcgtcggg tcggtagccg
     6541 atggcgccga gcttgcggag agcgtcgccg agcaccggaa cctcgggggc accgacgacg
     6601 cgggcgcgcc ggccgcaggc gacgtgcacg gcgcgcacga cggcgagcgg ctcgaggaac
     6661 ccgagccccc ggttcgcgac gagcagcgcg ggaccgacct gggggacgtg ttcgccgtgc
     6721 tcgacggtga cggggagggc cacgagcccg gccgtgaggt ccatgaggtg ggggtcggcg
     6781 ccgaactcgt cgacggcgta ctggccgcgc aggcgtcggc gtagcgccgc gggcagcgag
     6841 tcggtggcga ggacgcggtc ggtgtcgagc ccgtagttgg ggaccgggac ggtcacgcga
     6901 ctctccgggt cggctcgagt cgcagcaccg tcgcccattc gaacaggtcg acgaggacct
     6961 cttgggtggg gcgcagcgat ccgaggtcga gctcggcgac ggcgcgggct ccgtcgccgc
     7021 agcggccgtg gcggaggagc tcgacgacgt ggggcgggat gggtgcaccg gcgagctcgg
     7081 cggcctcggc cgcgagctcc cagccgaagc cgacgacggg gatcgggatc cggccgccga
//
LOCUS       DATCCH010000100         4581 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_176770, whole genome shotgun sequence.
ACCESSION   DATCCH010000100 DATCCH010000000
VERSION     DATCCH010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4581
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176770"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="VIJ44_02025"
     CDS             <1..956
                     /locus_tag="VIJ44_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012726000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY5164707.1"
                     /translation="AITDLYQRRFGVELDPETQACTTIGAKEGFSHLMWVLLGPGDAA
                     MVPSPSYPIHIWGPILAGAEVRHVRLGPEQDFFANLVETWEDSWPRPRVIVLSFPHNP
                     TTACVDLAFMTRIVDFARAHDVLLVHDFAYADLGFDGYEPPSILQVPGATDVAVELYT
                     LTKSFSMAGWRVGFLVGNAEVVAALGRLKSYLDYGTFQPIQIASIVAMNEAPDYPLGV
                     NAIYRRRRDALVDGLARIGWEIEKPRGTMFVWAPIPEPYEDLGSLDFSKVLVEEAKVA
                     VSPGVGFGAGGEGYVRFALVENEHRIGQAVRGIRRALTKLG"
     gene            1048..1755
                     /locus_tag="VIJ44_02030"
     CDS             1048..1755
                     /locus_tag="VIJ44_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013475739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY5164708.1"
                     /translation="MLYWTLKLVLTPLLRLGFRIQVEGRDHLPRRGPVILAANHRSFL
                     DSIFLPLAIGRRRMTFVAKAEYFEKRRTAWFFRGVGQIPIQRSGGSASDAALDAATDV
                     LRGGGAFGIYPEGTRTRDGVTHRGHTGVARLARSTGVPIVPVGLIGTDECQPTNAKLP
                     RFFRRVTIRFGEPIPADRYLADEGLALRQLTDEVMFEIVQLCGLYEYRDTYATKQAED
                     VPTPAARLAALPTARVA"
     gene            complement(1761..3254)
                     /gene="glpK"
                     /locus_tag="VIJ44_02035"
     CDS             complement(1761..3254)
                     /gene="glpK"
                     /locus_tag="VIJ44_02035"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEY5164709.1"
                     /translation="MTVVLAIDAGTTGVRTLGVDEDGSVRFGAYREFPQYFPQPGWVE
                     HDPVEILDATIATLAEVAAAVEAAGATVAAIGITNQRETTIVWDRTTGQPCHRAIVWQ
                     DRRTAGRCDQLRDEGHEPLIRSRTGLVLDPYFSATKLAWLFEDGGVERDANLAFGTVD
                     TWLTWCLTGGSDGGVHVTDPSNASRTLLYDIDELDWSDELAELFGVPASSLPAVLPSS
                     GHFGETVPDRAAGLRVPISGIAGDQAAALFGQACVEPGMTKNTYGTGSFVLMNLGPSH
                     PPPTEGLLTSVAWILGGDVTYALEGAVFVTGAAIQWLRDGLGIIADAAETGPLAASVA
                     DTGGVVFVPAFSGLGSPWWDPYARGTVLGITRGTTRAHLARAVVESMAWQTRDVIDAM
                     SQAAGRPVAELRVDGGASVMDLLCQLQADVLGVPVRRAAVQETTALGAAFLAGLGVGV
                     WSNPAEVGARWSADAEFEPAMARSEVEPRHRQWLRAVARSRGWARET"
     gene            complement(3251..3721)
                     /locus_tag="VIJ44_02040"
     CDS             complement(3251..3721)
                     /locus_tag="VIJ44_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164710.1"
                     /translation="MGRRLEQTVAATHGALVEGERVVAYGYCWAAQLRRIPLVFLGRR
                     RYLLLLTNRRVLLFNRRGRHHPRPNDLVIGKRYEWFKLGRVRRVRPLMQVLVTTGNGA
                     RMIFEFSPRRRELGRTLVGRLGDGTGSGEPGLGEATESHHVDHSAFWGPSTGSS"
     gene            3802..>4581
                     /locus_tag="VIJ44_02045"
     CDS             3802..>4581
                     /locus_tag="VIJ44_02045"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020115266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HEY5164711.1"
                     /translation="MRIGILTGGGDCPGLNAVIRAIVHKGEGVYGHEILGFRHGWRGV
                     LDNELTELTVASTRGLLHRGGTILGTSRTNPFQREDGVATVQATLTREHIEALIAIGG
                     EDTLGVAHKLGAEGVACIGVPKTIDNDLSATDVTFGFNTAVQIATDAVDRLHTTAESH
                     DRVMVVEVMGRHAGWLALSAGLAGGADLILIPEQPFDLDELCERLRHRHARGASFSIV
                     VVAEGAEPVAGSMTVRGGATDEFGHVRLGGIGNALAPEIEAR"
BASE COUNT          693 a         1670 c         1543 g          675 t
ORIGIN      
        1 ttgccatcac cgacctctac cagcgccggt tcggtgtcga gctcgacccc gagacgcagg
       61 catgcacgac catcggcgcc aaagaaggct tctcgcacct gatgtgggtg ctgctcggcc
      121 ccggggacgc cgcgatggtg ccgagcccct cgtacccgat ccatatctgg gggcccatcc
      181 tcgccggcgc tgaggtccgc catgtccggc tcggacccga acaggacttc ttcgcgaacc
      241 tggtcgagac ctgggaggac tcctggcccc gcccgcgggt catcgtgctc tcgttcccgc
      301 acaacccgac caccgcgtgc gtcgacctcg ccttcatgac ccgcatagtc gacttcgccc
      361 gggcacacga cgtgctcttg gtccacgact tcgcctacgc cgatcttggg ttcgacggct
      421 acgaaccccc gtcgatcctc caggtgccgg gcgccaccga cgtcgccgtc gagttgtaca
      481 ccctcacgaa gtcgttctcg atggccggct ggcgggtcgg gttcctggtc ggtaacgccg
      541 aggtcgtcgc cgcgcttggc cggttgaaga gctacctcga ctacgggacg ttccagccca
      601 tccagatcgc ctcgatcgtc gctatgaacg aagcgcccga ctacccgctc ggggtcaacg
      661 cgatctaccg gcgtcgacgc gacgccctcg tggatggcct ggcccggatc ggctgggaga
      721 tcgagaaacc gcgcgggacc atgttcgtgt gggcgccgat ccccgagccg tacgaggacc
      781 tggggagtct cgacttctcc aaggtgctcg tcgaggaagc caaggtcgcg gtgtcacccg
      841 gcgtcgggtt cggtgccggc ggggagggct acgtgcggtt cgcgctggtc gagaacgagc
      901 atcgcatcgg ccaggcggtg cgcgggatcc ggcgggccct gaccaagctc ggctgagcct
      961 ccggcggcat cggcggtcga ggtcagcgtg gtctcggcac cgggctgcgc tcggtaacgt
     1021 gacgcccgtc accaaggggt gaaggccatg ctctactgga cgctcaagct cgtcctgacg
     1081 ccgctgcttc gcctgggctt ccgcatccag gtcgagggtc gcgatcatct gccgcgccgc
     1141 ggcccggtga tcctcgccgc caatcaccgg tcgttcctcg actcgatctt cttgccgctc
     1201 gcgatcggtc ggcgccgcat gaccttcgtc gccaaggccg agtacttcga gaagcgtcgc
     1261 acggcgtggt tcttccgcgg tgtcggccag atcccgatcc aaagaagcgg cgggagcgcg
     1321 agcgacgccg cgctcgacgc cgccaccgac gtcctccggg gcggcggagc gttcgggatc
     1381 tacccggaag ggacccgcac tcgcgatggc gtcacccacc ggggccacac cggtgtcgct
     1441 cgcctcgctc gctccaccgg ggtgccgatc gtcccggtcg ggctcatcgg taccgacgag
     1501 tgtcagccca cgaacgccaa gcttccgcgg ttcttccgac gggtcacgat ccgcttcggg
     1561 gagccgatcc ccgccgatcg gtacctcgcc gatgagggcc tggcgctacg ccagctcacc
     1621 gacgaggtga tgttcgagat cgtgcagctc tgcggcctct atgagtaccg ggacacgtac
     1681 gcgacgaaac aggccgaaga cgttcccacc ccggcggccc gcctggccgc gctccccact
     1741 gcccgcgtcg cgtagcgccg tcaggtttcc cgagcccagc cgcgggaccg ggccacggcc
     1801 cgcagccact gccggtgccg cggctcgact tcggatcggg ccatcgccgg ctcgaactcg
     1861 gcgtcggcgc tccagcgtgc cccgacctcg gccgggttcg accacacccc gaccccgagg
     1921 ccagcgagaa acgcggcgcc gagcgcagtg gtctcttgga ccgcggcgcg gcgcaccggc
     1981 acgccgagca cgtcggcctg cagctggcac agcagatcca tgacgctggc ccccccatcg
     2041 acgcgcagct ccgccaccgg ccgccccgcg gcctgactca tcgcatcgat cacgtcgcgg
     2101 gtctgccagg ccatcgattc cacgaccgct cgggccagat gggcccgggt cgtgccccgg
     2161 gtgatcccga gcaccgtccc ccgcgcgtac gggtcccacc acggcgaccc gagcccgctg
     2221 aacgcgggga cgaacaccac cccaccggtg tcggcgacgc tggcggccag cggcccggtc
     2281 tcggcggcgt cggcgataat ccccaggcca tcgcgcaacc actggatcgc ggcgccggtg
     2341 acgaacaccg ccccttcgag cgcgtaggtg acgtcaccgc cgaggatcca cgccacgctg
     2401 gtgaggagcc cctcggtcgg cggtggatgc gacggcccca agttcatgag gacgaacgac
     2461 cccgtcccgt aggtgttctt cgtcatcccc ggctcgacgc aggcctgccc gaacagcgcc
     2521 gccgcctggt ccccggcgat gccgctgatc gggacccgaa ggcccgccgc ccggtccggg
     2581 accgtctccc cgaagtgtcc gctgctgggc aagaccgccg ggagggacga ggccgggacg
     2641 ccgaacagct cggccagctc gtctgaccag tcgagctcgt cgatgtcgta caggagcgtc
     2701 cgcgacgcgt tcgacggatc ggtgacgtgc acaccaccgt cggatccgcc ggtgaggcac
     2761 cacgtcagcc acgtgtcgac ggtcccgaac gccaagttcg cgtcccgctc gacgccgcca
     2821 tcctcgaaga gccacgccag cttcgtcgcc gagaagtacg ggtcgaggac cagcccggtt
     2881 cgtgaccgga tcagcggctc gtggccctcg tcgcgcagtt gatcgcagcg gcccgcggtg
     2941 cggcggtcct gccagacgat ggcgcggtgg cagggctgac ccgtcgtgcg gtcccagacg
     3001 atcgtcgtct cacgttgatt ggtgatcccg atcgcggcga cggtcgcccc ggccgcttcg
     3061 accgcggcgg cgacctcggc gagcgtggcg atcgtcgcgt cgaggatctc gacggggtcg
     3121 tgttcgaccc atcccggttg ggggaagtac tgcgggaact cgcgatacgc gccgaaccgg
     3181 acggaaccat cctcgtcgac gccgagggtg cggacgcccg tggtgccggc gtcgatcgcg
     3241 aggacgacgg tcacgacgag ccggtcgaag gtccccagaa cgccgagtgg tcaacgtggt
     3301 ggctttcggt ggcctcaccg agccccggtt cgcccgaccc ggtgccatcg ccgaggcgcc
     3361 cgacgagggt gcggcccagc tcgcgacggc ggggcgagaa ctcgaagatc atgcgggcgc
     3421 cgttcccggt ggtgaccagc acctgcatca acggccgaac ccgacgcacc cggcccagtt
     3481 tgaaccactc gtatcgtttc ccgatcacca ggtcgttcgg ccggggatga tgacgccccc
     3541 gccggttgaa caacaagacc cgacgattgg tgagcaacag gaggtaccgg cgccgcccga
     3601 ggaataccaa cgggattcgc cgcagctgcg cggcccagca gtagccgtag gcgacgaccc
     3661 gctcgccctc gacgagggcc ccgtgcgtcg ccgcgacggt ctgttcgaga cggcggccca
     3721 tcgaaggcga ggctaccggc cacgtcgatc gtcggcgggc tgcgccagga tggtgacgca
     3781 cctgagcgga ggagaacggc gatgcggatc gggatcctga ccggtggcgg cgactgtccg
     3841 ggactgaacg cagtcatccg ggccatcgtc cacaaaggcg agggcgtcta cgggcacgag
     3901 atcctcggct tccgccacgg gtggcgcggt gtcctcgaca acgaactcac cgagctgacg
     3961 gtcgcctcga cccgcgggct cctccaccgc ggcggcacga tcctcggcac ctcgcgcacg
     4021 aacccgttcc agcgcgaaga cggggtcgcg accgtccagg ccaccctcac ccgcgagcac
     4081 atcgaggcgc tcatcgcgat tgggggcgag gacaccctcg gtgtcgccca caagctgggc
     4141 gccgaggggg tggcatgcat cggggtgccc aagacgatcg acaacgacct ttccgcgacc
     4201 gatgtcacct tcgggttcaa caccgcggtg cagatcgcca ccgacgccgt cgaccggctc
     4261 cacaccaccg ccgagagcca cgaccgggtc atggtggtgg aggtcatggg tcgccacgcc
     4321 ggctggctcg cgctgtcggc gggcctcgct ggtggtgccg acctcatctt gattccggag
     4381 cagccgttcg acctcgacga gctgtgcgaa cggctccgac accgtcacgc gcgcggcgcg
     4441 agcttctcca tcgtcgtcgt cgccgaaggt gccgaaccgg tcgcggggtc gatgaccgtc
     4501 cggggcggcg ccaccgacga gttcggccac gtgcgtctcg gtgggatcgg caacgcgctc
     4561 gcccccgaga tcgaggcccg g
//
LOCUS       DATCCH010000101         2012 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_550029, whole genome shotgun sequence.
ACCESSION   DATCCH010000101 DATCCH010000000
VERSION     DATCCH010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550029"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..1117
                     /locus_tag="VIJ44_02050"
     CDS             128..1117
                     /locus_tag="VIJ44_02050"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY5164712.1"
                     /translation="MPDRDQRRVGGAASDALRRVSLGRELQRALAGGELQLAYQPVVN
                     MLDGGVCGVEALLRWEHPHLGLMWPGEFLDAVHGTELGTQVLHWVMNEALRQGSEWRT
                     RFPDHPIWVSVNVVGHDFVADGLLSDVAEILEANQLDPSALALEVKERDVFDDRYGLV
                     RERLDTIKRAGIGVLVDDFGNAHAALMTALEAWPPNVRKVYPAFVAQVVTQAYEATVA
                     SMNALRGWPIDVLKLDRVLLDRLFTDRGSEPLVDSLVRLARSLGLRTLATSIESPSEA
                     RQLYQLGCDMAQGYHFHRPQTPEYVDVLLAEQDHEPSTPRGLGDGQALHVGHG"
     gene            1286..>2012
                     /locus_tag="VIJ44_02055"
     CDS             1286..>2012
                     /locus_tag="VIJ44_02055"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY5164713.1"
                     /translation="MRRWWAAAIAALSVIGVGAGGIPASAAATPKSPVKHVFVVVLEN
                     ASASEIASPTFKTTMPYLSSLAAKGVSLDQMYAIDHVSLGNYIGMTSGYEANAKTKAD
                     CTQYDCVYPVGQDDNVANQLEAQHLTWKGYMDGMTQPCQKPPTQGAPDPYQVGYATRH
                     NPFMYYASIVDNATRCRSHVVPFPQLATDLAANRVPNYSFISPSTCNDAHDRGSACGL
                     EQADVWMSQHVQPILKSKAYKDGG"
BASE COUNT          347 a          733 c          641 g          291 t
ORIGIN      
        1 ccgatgcgag caggcgacct agtcgacgtt ggtgctgttg tcgctcaagg tgggctggtc
       61 atcgcccgat gtttcggtcc gagggcgacc cccggggcgg gggttcggag cgaggttgat
      121 gatggtgatg ccggatcgag accagcgccg cgtcggaggc gccgcatccg acgccctgcg
      181 tcgcgtcagc ctcggccgcg agttgcagcg ggccctcgcc ggcggggagc tccagctcgc
      241 gtaccagccc gtcgtcaaca tgctcgatgg cggcgtgtgt ggcgtcgagg ccttgctccg
      301 atgggaacat ccccacctcg ggctcatgtg gcccggcgag ttcctcgacg cagtgcacgg
      361 caccgagctc ggcacccagg tgctgcactg ggtcatgaac gaggcgctac gtcaagggtc
      421 ggagtggcga acccgctttc ccgaccatcc catctgggtg tcggtcaacg tcgtcggtca
      481 cgacttcgtc gccgacgggc tgctcagcga cgtcgcggag atcctggagg ccaaccagct
      541 cgaccccagc gccctcgcgc tcgaggtcaa ggagcgcgac gtcttcgacg accggtacgg
      601 cctcgtccgc gagcgactcg acacgatcaa gcgggctggg atcggtgtcc tcgtcgacga
      661 cttcggcaat gctcacgccg cgctgatgac cgcgctcgaa gcgtggccgc cgaacgtgag
      721 gaaggtgtac cccgcgttcg tcgcccaggt cgtcacgcaa gcgtacgagg cgaccgtcgc
      781 gagcatgaac gccctccgag gctggccgat cgacgtgctc aagctcgacc gcgtcctcct
      841 ggaccgcctg ttcaccgacc gagggtccga gcccctcgtc gacagcctcg tgcgcctggc
      901 ccgcagcctc ggcctgcgaa ccctggcgac gagcatcgaa tcaccaagcg aggcccgcca
      961 gctctaccag ctgggctgcg acatggccca ggggtaccac ttccatcggc ctcagacccc
     1021 cgagtacgtc gacgtcctcc tcgccgagca ggaccacgag ccgtcgacgc cccgcggcct
     1081 cggcgacggc caggcgctgc acgtcggaca cggttaaggc ccaccgacgc catctcgcgc
     1141 gccccgttcg aggccgcgtc gcgccggtga cgaacccgcg aacacggcgc gacaacctcc
     1201 ggacgaggca ccggaccggg cccatcgacc gctgagccga ggtacggtcc gggctcatcc
     1261 gcagcggagg gaccgggcag tgggaatgcg acggtggtgg gcggccgcga tcgcggcatt
     1321 gagcgtcatc ggagtcggtg caggcggcat cccggcctcg gccgccgcga ccccgaagtc
     1381 gccggtgaag cacgtcttcg tcgtcgtgct cgagaacgcc agcgccagcg agatcgccag
     1441 ccccaccttc aagaccacga tgccgtatct gtcgtcgctc gccgcgaaag gcgtctccct
     1501 cgaccagatg tacgccatcg accacgtcag tctgggcaac tacatcggga tgacgagcgg
     1561 gtacgaggcc aacgccaaga ccaaggccga ctgcacccag tacgactgcg tctaccccgt
     1621 cggccaagac gacaacgtcg ccaatcagct cgaggcccag cacctcacct ggaagggcta
     1681 catggacggc atgacccagc cgtgccagaa gcccccgacc caaggcgccc ccgacccgta
     1741 ccaagtcggc tacgccaccc gccacaaccc attcatgtac tacgccagca tcgtcgacaa
     1801 cgccactcga tgccgctccc acgtcgtgcc gttcccgcag ctcgcgacgg acctcgccgc
     1861 gaaccgggtc cccaactact cgttcatctc gccgagcacc tgcaacgacg cccatgaccg
     1921 cgggtcggcc tgcggtctgg aacaagctga cgtttggatg agtcagcacg tccagcccat
     1981 cctcaagtcg aaggcctata aggacggcgg cg
//
LOCUS       DATCCH010000102         5988 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_467950, whole genome shotgun sequence.
ACCESSION   DATCCH010000102 DATCCH010000000
VERSION     DATCCH010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5988
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467950"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..633)
                     /locus_tag="VIJ44_02060"
     CDS             complement(64..633)
                     /locus_tag="VIJ44_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019135032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEY5164714.1"
                     /translation="MGLEQFPHNFLTARLEDLVQWARERSVFPATFGLACCAMEMMAT
                     GAAHYDLARYGMEVWRASPRQADLMIVAGRVSQKMAPVLRQVYDQMVDPKWVISMGVC
                     ASTGGMFNNYALVQGVDQIVPVDVYVPGCPPGPETLFHGILTLHHQIRTGDLLRRRDA
                     IGTGAGIQVEQRDGTPSHVQPIPIRRKSA"
     gene            complement(637..1101)
                     /locus_tag="VIJ44_02065"
     CDS             complement(637..1101)
                     /locus_tag="VIJ44_02065"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEY5164715.1"
                     /translation="MEQYLPILAMVVLVLLFVTLSFVASALLGPRKRPTSAKEAPYEC
                     GIVPETEPAERFPVRFYLVAMAFIVLDVEIIFIYPFTTVLKALGTYGLVAMGVFLLVL
                     LVPFGYLLSTGAVDWGPVRQVTDRIAHRMLRATGTPGRAGLDPAEQPTEEAA"
     gene            complement(1222..2475)
                     /locus_tag="VIJ44_02070"
     CDS             complement(1222..2475)
                     /locus_tag="VIJ44_02070"
                     /inference="COORDINATES: protein motif:HMM:NF017634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HEY5164716.1"
                     /translation="MGVRDRFDVLIVGGGPSGAAAACWLAEAGHRVLVVERKQFPRDK
                     TCGDGLTPRAVRQLEDLGLADELEPHLRFEGLRSIAHGITLELAWPEHPEYPPYGYVV
                     RRRDLDAMVANRAVKAGATLWTGTEAVEPVADGGLVTGAVLHRGVDDSHETVHARYVI
                     VADGANSRFGRALGTSRDRTYPLGMAVRGYFQSPFHDEPWIESHLDLRDKDGNHLPGY
                     GWIFPVGDGTINVGVGLLSTFAGWKDVNTTKLMEAFCETAPARWGISPDTACGAPTGG
                     KLPTGLSVFPRVGPTWCTVGDAAGSVNPFNGEGISVAYETGRLAADAIDIALRTGDGL
                     ALQRYPERLEATYGLYFKVARAFVRAIGNPAVMRELTRVGFMSRSLMEWVLRIMANLL
                     RPEELGPAEVAYRLVERLVAFAPEP"
     gene            2637..3314
                     /locus_tag="VIJ44_02075"
     CDS             2637..3314
                     /locus_tag="VIJ44_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164717.1"
                     /translation="MPNLMPDWLPWIRPAGRLLWGVFALGVGMSFFLVLLRRPLMKRP
                     FPSRVGYLAFPVILGVGIVGAKVFTSAQTIIVWGTALALIGHGLLMVASGKARDAEVP
                     ATWAECFAGAMGTFAIFVVGYAIVPSEWLNYANSYIQWGDNTKFVWKSSQNMLFLPFR
                     WPFSLDFPALRDIVVTLIYVVFLGINLKLWVMWQRRHEVREQAATASADAPARRSRFG
                     RPLRAKA"
     gene            3325..3738
                     /locus_tag="VIJ44_02080"
     CDS             3325..3738
                     /locus_tag="VIJ44_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEY5164718.1"
                     /translation="MARTDANPPMPDFVTDYQLQEVDPAYLTQSVKPKQFLHIDQEEC
                     ILCEGCVDICPWKCIHMVSVGAIAEALGTEQPGEDPKDHVAFIVDEDVCTRCALCVDR
                     CPTGVIILGKLGNLPPDGDRHTRTARYGYAYGVRF"
     gene            3870..4586
                     /gene="extP"
                     /locus_tag="VIJ44_02085"
     CDS             3870..4586
                     /gene="extP"
                     /locus_tag="VIJ44_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenite/tellurite reduction operon b-type
                     cytochrome ExtP"
                     /protein_id="HEY5164719.1"
                     /translation="MRGSQAWNSIFRPGSVYRRGYTDSPRNRSYVIMNNVLYHLHPVK
                     VKRHAVKVSYTLCLGGLSFFLFILLTVTGIFLMFFYRPTTASAWNDIKTLETAVTFGS
                     LVRNMHRWAAHLMVLSVFLHMCRVFYHGAYKAPREFNWVIGVILLTLTLLLSFTGYLL
                     PWDQLALWAVTVGTNMMGYTPVFGSSVKYVLLGSKEISGSTLLRWYVLHVLLLPFVIV
                     IFMAVHFWRVRKDGGVSGPL"
     gene            4609..5454
                     /locus_tag="VIJ44_02090"
     CDS             4609..5454
                     /locus_tag="VIJ44_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinol-cytochrome c reductase cytochrome b
                     subunit"
                     /protein_id="HEY5164720.1"
                     /translation="MADVPEHLLRRSLERRKALGLPVPGEEGGEAASEEPPAAEAATP
                     ESGAPAPDAGGGDGGGGPDVETAGGAKIPAHLLERSKQRRAAAGGGEAAAAAAGAGTA
                     TAVAAPPAPTATGPGGHTQRLLTVVKSGSIQQTKAEAQDKVHTWPHLLVIEFAAVLIV
                     LAALTIFSALVKAPLLGLSDVNKTPNPSKAPWYFLGLQELLTMFHPMVAGVTIPGIGL
                     GLLAVAPFVDKNPSHFPKDRKYAIAIFTMFIMLWAVLVIIGSFFRGEGFNFVFPWNNG
                     IFFEL"
     gene            5468..>5988
                     /locus_tag="VIJ44_02095"
     CDS             5468..>5988
                     /locus_tag="VIJ44_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164721.1"
                     /translation="MAVIIVGVIVLIALAGLVVLGTARARANTGRLSRETRQRDVSGP
                     PPDVRPEPAEGDEIDEEARARADDSRRAIESGGALPVPAGGRGVARYEPVDEDELGVT
                     RRQFFNRGILTGLLLGVSGFGAASLAFLWPAAGGGFGGKINIGSKADVDTAIAKKTPF
                     YNATAKVYINPYP"
BASE COUNT          951 a         2021 c         2066 g          950 t
ORIGIN      
        1 cgaggggctc gcccgcggcg gcgtggtcag ggtccgtggc catttcggtc ctcggggctg
       61 agttcaggcc gatttccggc ggatcgggat gggctggacg tgcgacgggg tgccgtcgcg
      121 ctgctcgacc tggatccccg cgccggtgcc gatcgcgtca cggcggcgga ggagatcgcc
      181 ggtgcggatc tgatgatgga gggtcaagat cccgtggaag agcgtctcgg gccccggcgg
      241 gcatcccggc acatacacgt ccaccgggac gatctggtcg acaccttgga ccagcgcgta
      301 gttgttgaac atcccgccgg tgctggcgca cacccccatc gagatgaccc acttcgggtc
      361 gaccatctgg tcatagacct ggcgcagcac cggcgccatc ttctggctga cccggcccgc
      421 gacgatcatg aggtcggctt ggcggggact ggcacgccac acctccatcc cgtaccgggc
      481 gaggtcgtag tgcgcggcgc cggtcgccat catctccatc gcgcagcagg ccagaccgaa
      541 cgtggcgggg aacaccgacc gttcacgcgc ccactgcacg agatcctcga ggcgggcggt
      601 caggaagttg tgcgggaact gctcgagacc catgcgctac gcggcttcct cggtcggttg
      661 ttccgccggg tcgaggccgg cccggcccgg ggtgccggtc gcgcgcagca tccgatgcgc
      721 gatccggtcg gtcacctgcc ggacgggacc ccagtccacg gcaccggtcg acagcagata
      781 gccgaacggg acgaggagaa cgaggaggaa gacacccatc gccacgagac cgtaggtacc
      841 gagggccttg aggacggtcg tgaacgggta gatgaagatg atctcgacat cgaggacgat
      901 gaacgccatc gcgacgagat agaaacgcac cgggaaacgc tcggcgggct cggtctcggg
      961 cacgatgccg cactcgtagg gcgcttcctt ggccgacgtc ggccgcttgc gggggccgag
     1021 cagcgccgac gccacgaacg agagcgtgac gaacaacaag accagcacga ccatcgcgag
     1081 gatcggcagg tactgctcca ccctggtccc ctcccgggag tggtccggtg cacacgcggt
     1141 cgcgcgcagg ctatccgacc cccctgcggg caggggaagg gatcccgccg caaccggcgc
     1201 cggtcggacg cggcggcggg cctaaggctc gggggcgaac gcgaccaggc gctcgacaag
     1261 gcggtacgcg acctccgcgg gcccgagctc ctcggggcgg agcaagttgg ccatgatccg
     1321 cagcacccac tccatgagcg accgcgacat gaacccgacc cgggtgagct ctcgcatgac
     1381 cgcggggttg ccgatggccc gcacgaaggc tcgcgccacc ttgaagtaga gcccgtacgt
     1441 cgcttcgagc cgttccgggt agcgctgcag ggccaggccg tcgccggtgc gcagcgcgat
     1501 gtcgatggcg tcggcggcca ggcgaccggt ctcgtacgcg acggagatgc cctccccgtt
     1561 gaaggggttc acggacccgg cggcgtcgcc gacggtgcac caggtcgggc cgaccctcgg
     1621 gaacaccgac agcccggtgg gaagcttccc gcccgtcggc gcgccgcagg cggtgtcggg
     1681 cgagatgccc cagcgggcgg gcgcggtctc gcagaacgct tccatcagct tggtggtgtt
     1741 cacgtccttc cagccggcga aggtggacag gaggccgacg ccgacgttga tggtcccatc
     1801 gccgaccggg aagatccacc cgtagcccgg caggtgattg ccgtccttgt cgcgcaggtc
     1861 gaggtggctc tcgatccacg gctcgtcgtg gaacgggctc tggaagtagc cccggacggc
     1921 catgccgagc gggtacgtcc ggtcccggct ggtaccgagc gcccgcccga agcgtgagtt
     1981 cgctccgtcg gcgacgatga cgtaccgggc gtggaccgtc tcatggctgt cgtccacccc
     2041 ccggtgcagc accgcgccgg tgacgaggcc accgtcggcg accggctcga ccgcctcggt
     2101 gccggtccag agggtggcgc cggccttgac ggcccggttg gcgaccatgg cgtcgaggtc
     2161 gcgccggcgc accacgtatc cgtacggcgg gtattcggga tgttcgggcc aggccagctc
     2221 gagggtgatg ccgtgggcga tcgagcgcag gccctcgaag cgcaggtgcg gctcgagctc
     2281 gtcggcgagg ccgagatctt cgagctggcg gacggcgcgg ggggtcagcc cgtccccgca
     2341 ggtcttgtcg cgggggaact gcttgcgttc gacgacgagg acccgatggc ccgcctcggc
     2401 gagccagcac gccgcggcgg cacccgaggg gccacccccg acgatcaaca cgtcgaatcg
     2461 gtcgcgaacg cccatcgccg accatcgtgc catcgcgggc ggatgggttc accacccagg
     2521 cgcggcccga tatgccgttt ccacagttac cgtggtagga aaagcctacg gggcaagggt
     2581 cctgggggag tctcggctct tcgagacaca cgctccggag ggaggacacc ggcggcatgc
     2641 ccaacctgat gcccgactgg ctgccgtgga ttcgtcctgc cggtcggctc ctctggggcg
     2701 tgttcgccct cggggtcggc atgagcttct tcttggtgct gttgcggcgc ccgttgatga
     2761 agcgaccgtt cccgagccgg gtcggctacc tcgcgttccc ggtcatcctg ggcgttggga
     2821 tcgtcggcgc aaaggtgttc acgagcgcgc agaccatcat cgtgtggggc acggcgctgg
     2881 ccctgatcgg tcatggcctc ttgatggtcg cgagcggcaa ggcccgcgac gccgaggtgc
     2941 cggcgacctg ggcggaatgc ttcgccggcg cgatgggcac cttcgctatc ttcgtggtcg
     3001 gttacgcgat cgtccccagc gagtggctca actacgccaa ctcctacatc cagtggggtg
     3061 acaacacgaa gttcgtgtgg aagagcagcc agaacatgct gttcctcccg ttccgctggc
     3121 cgttcagcct cgactttccc gcgctgcgcg acatcgtcgt cacgttgatc tacgtcgtct
     3181 tcctcggcat caacctcaaa ctgtgggtca tgtggcagcg gcgccacgag gtgcgcgagc
     3241 aggcggccac ggccagtgcg gacgctccgg ctcgccggtc gcgcttcggg cgcccgttgc
     3301 gggcgaaggc gtaggagttc gatcatggcg cgaaccgacg cgaacccgcc gatgccggac
     3361 ttcgtcaccg actaccagct ccaagaggtg gatccggcgt atctgaccca gtcggtcaag
     3421 ccgaagcagt tcctccacat cgaccaggag gagtgcatcc tttgcgaggg atgcgtcgac
     3481 atctgcccgt ggaagtgcat ccacatggtg tcggtagggg ccatcgccga ggcgctcggg
     3541 accgagcagc cgggcgagga tccgaaagac catgtggcgt tcatcgtcga tgaggacgtc
     3601 tgcacccgct gcgccctctg cgtcgaccgc tgtccgacgg gtgtgatcat cctcggcaag
     3661 ctcggcaacc tgcccccgga cggcgaccgg cacacccgaa cggctcgata cggctacgca
     3721 tacggagtgc ggttctaggc gtccaccggc gccgacgggg acgaagaatc caacgaggag
     3781 acaggttcgt ggccaagggg aacgggacga acggcaacgg gcacgccggg cagcgggtcg
     3841 cggccgcggt cgacaaagtc gacgagcgga tgcggggctc gcaggcctgg aactcgatct
     3901 tccggccggg gtcggtgtac cggcggggct acaccgacag cccccgcaac cggtcgtacg
     3961 taatcatgaa caacgtcctg taccaccttc acccggtgaa ggtgaagcgg cacgcggtga
     4021 aggtgtccta caccctgtgc ctcggcggcc tgtcgttctt cctgttcatc ctcttgacgg
     4081 tcaccggcat cttcttgatg ttcttctacc ggccgacgac cgcgtcggct tggaacgaca
     4141 tcaaaaccct cgagactgcg gtgaccttcg ggtcactggt ccgcaacatg caccggtggg
     4201 ctgcgcacct catggtgctc tccgtcttct tgcacatgtg tcgcgtcttc taccacgggg
     4261 cctacaaggc tccccgcgag ttcaactggg tcatcggcgt catcttgttg acgctcacgc
     4321 tgctgctgtc cttcaccggc tacctgctcc cctgggacca gctcgctcta tgggcggtca
     4381 cggtggggac gaacatgatg ggctacacgc ccgtgttcgg cagcagcgtc aaatatgtgc
     4441 tgctgggatc gaaggagatt tccggcagca ccctccttcg atggtacgta ctccatgtct
     4501 tgctgctgcc gttcgtcatc gtcatcttta tggccgtcca cttttggcgg gtccgcaaag
     4561 acggcggcgt ctcgggtccc ctgtaggtcg acggagtagg agcgcgaagt ggccgacgtc
     4621 cccgaacacc tcctgcgccg ctcactcgag cgccgcaagg cgctaggtct ccccgtcccc
     4681 ggggaagaag gcggcgaggc ggcatccgag gagccgcccg cggccgaagc cgccacgccc
     4741 gaatccgggg cgccggcgcc cgacgcgggg ggtggcgacg ggggcggcgg tccggacgtg
     4801 gagaccgccg gcggcgccaa gatccccgcc catctgctcg agcgttcgaa gcaacgccgg
     4861 gccgccgcgg gcggcggcga agccgcggcg gcagctgcgg gcgcggggac cgcgaccgcg
     4921 gtggcggccc cgccggcacc gaccgccacc ggccctgggg gccacaccca gcggctgctg
     4981 accgtcgtga agtccgggtc gatccaacag accaaggccg aggcccagga caaggtgcac
     5041 acctggccgc acctgctggt gatcgagttc gcggcggtcc tgatcgtcct ggcggcgctc
     5101 accatcttct cggcgttggt gaaggcgccc ctgctcggcc tctccgacgt gaacaagacc
     5161 ccgaacccgt cgaaggcacc gtggtacttc ctgggcctcc aggagctcct gacgatgttc
     5221 caccccatgg tcgcgggggt gaccatcccc ggcattggcc tcggactcct cgcggtcgcg
     5281 cccttcgtcg acaagaaccc gtcgcatttc ccgaaggacc gcaagtacgc gatcgcgatc
     5341 ttcaccatgt tcatcatgct gtgggcggtg ctcgtcatca tcgggtcgtt cttccgcggc
     5401 gagggattca acttcgtgtt cccgtggaac aacggcatct tcttcgagct gtaggtagga
     5461 gcagaacgtg gcagtcatca tcgtcggcgt gatcgtcctg atcgccttgg ctggactcgt
     5521 cgtgctcggc acggctcggg cccgggcgaa caccgggcgt ctcagccgcg agacccgcca
     5581 acgcgatgtg tcgggaccgc ccccggatgt ccggcccgaa ccggccgagg gcgacgagat
     5641 cgacgaagag gcccgggccc gagccgacga ctcccgacga gcgatcgaga gcggcggagc
     5701 gctcccggtc cccgcgggtg gtcgcggcgt cgcccgctac gagccagtcg acgaggacga
     5761 gctcggcgtc actcgccgcc agttcttcaa ccgcgggatc ctgaccgggc tgctcctcgg
     5821 cgtcagcggc ttcggcgccg cgtcgctcgc gttcttgtgg ccggccgcgg gcggtggatt
     5881 cggcgggaag atcaacatcg ggtcaaaggc cgacgtcgac accgcgatcg ccaagaagac
     5941 cccgttctac aacgcgacgg cgaaggtcta catcaaccca tacccgaa
//
LOCUS       DATCCH010000103         3164 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_420270, whole genome shotgun sequence.
ACCESSION   DATCCH010000103 DATCCH010000000
VERSION     DATCCH010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420270"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..363
                     /locus_tag="VIJ44_02100"
     CDS             166..363
                     /locus_tag="VIJ44_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164722.1"
                     /translation="MSASDLAELSTLRAQLDELTVRVASVAERYDDTPDSAIAAELFA
                     AERLLIGAGRSLDRAIARLTA"
     gene            complement(353..997)
                     /gene="nth"
                     /locus_tag="VIJ44_02105"
     CDS             complement(353..997)
                     /gene="nth"
                     /locus_tag="VIJ44_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012308051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEY5164723.1"
                     /translation="MTLPRGARQRAAVVVERLTSEYPDPRCALVHDNTFQLLVATILS
                     AQSTDEMVNKVTPALFARYSTAADLADADPDELETLIHSTGFFRSKAKSLMGMAAAVE
                     ERFDGGVPRALEDLVTLPGVGRKTGNVVRSVGYDLPGLPVDTHVGRLARRLRLTSETD
                     PVKAEHDLNRLIAEPERGRFSLRLIEHGRRVCLARRPRCDVCVLADICPSAFKL"
     gene            1033..1956
                     /gene="mshD"
                     /locus_tag="VIJ44_02110"
     CDS             1033..1956
                     /gene="mshD"
                     /locus_tag="VIJ44_02110"
                     /EC_number="2.3.1.189"
                     /inference="COORDINATES: protein motif:HMM:TIGR03448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035447 - mycothiol synthase activity
                     [Evidence IEA];
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol synthase"
                     /protein_id="HEY5164724.1"
                     /translation="MVDVQLRRPVAPSDVDVLRRVADDAGRAAGHAVVGDTVWRDLAD
                     PSPDTAIIVARDGESTVGAMHIGSNDSRTTPHTTLSVAVAPGAPHGEVVAAMTTPALA
                     DRRAHGGGHVELWVLGTDRSWDSRARRLGLDPARELRQLRVRLPVTEPVTWPPGVTVR
                     RFEVGRDEAAWVAVNNRAFARDPDQGGWVQATLRRREQEEWFDAAGFLLAEDDRGLAG
                     FCWTKLHPAAPPIEPVALGEVYVIGVEPGRQGSGLGRSLVLAGLADVHDRRGAPVGML
                     FVDTTNDAALRLYESIGFTVTRVDRAYACEL"
     gene            1944..3020
                     /gene="rlmN"
                     /locus_tag="VIJ44_02115"
     CDS             1944..3020
                     /gene="rlmN"
                     /locus_tag="VIJ44_02115"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010119001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEY5164725.1"
                     /translation="MRALTRYGASRAELASLLDDEPRYRVDQVWDALWRRRVALESAT
                     ELPGALRTRLADLLPLALAPVTELVSADELTRKWLWAAGDAQVETVLMRSPRRASVCV
                     SSQAGCAMGCTFCATGQAGFDRHLRADEIVEQVLRAEHASPQRVTNVVYMGMGEPLAN
                     YDEVWASVVTLKEALGLSARRLTISTVGVVPGMRRLATEGLPVTLAVSLHAPDDALRA
                     TLVPLNHRYPIAEVLDAAADVAGASGRRVTFEYAGIAGVNDQPAHAEALARLLGSWPG
                     VGGSHVNLIPLNATAGFPGRASALPALHDFARRLRARGVTATVRRTRGADIDAACGQL
                     RRRTLIPPERSGSTRMGPCASTGS"
BASE COUNT          432 a         1160 c         1110 g          462 t
ORIGIN      
        1 acgggcgcgg tcggctcctc gggcggggcg agcgcgaacg cgaggttccc ggtgaacgcg
       61 cggaggtcga gcaggtcgag cacggtggga gtcatcgtaa ccgtgggtcg tcactccacc
      121 gttccgcttt gcctacccgt cgatgagacg acaggctggg gcgcgatgag cgcgtccgac
      181 ctcgccgagc tttcaacctt gcgcgcgcag ctcgacgagc tgaccgtacg agtggcgagc
      241 gtcgctgagc gttacgacga cacgccggac tcggcgatcg ccgcggagtt gttcgcggcg
      301 gagcggctgc ttatcggcgc gggacgatcg ttggatcgcg ccatcgcccg gctcacagct
      361 tgaacgcgct gggacagatg tcggcgagca cgcacacatc acagcggggt cggcgcgcca
      421 gacacacccg gcgaccgtgc tcgatcaggc gcagcgagaa ccgcccccgc tccggttccg
      481 cgatcagccg gttgagatcg tgctccgcct tcaccgggtc ggtctccgac gtcaggcgga
      541 gccgacgcgc caaccggccg acgtgggtgt cgaccggcag gcccgggagg tcgtagccga
      601 ccgagcgaac cacgttgccg gtcttgcggc ccacgccggg cagcgtcacc aagtcctcca
      661 gcgctcgggg caccccgccg tcgaagcgct cctcgaccgc ggcggccatg cccatcaggc
      721 tcttggcctt ggagcgaaag aaccccgtcg agtggatgag ggtctcgagc tcgtcggggt
      781 cggcatcggc gagatccgcc gccgtcgagt agcgcgcgaa cagggcgggg gtcaccttgt
      841 tgaccatctc gtcggtcgac tgggccgaaa ggatcgtggc gacgagcagc tgaaaggtgt
      901 tgtcgtgcac gagggcgcag cgagggtcgg ggtattccga cgtgagccgt tcgaccacga
      961 ccgcagcgcg ttgtcgtgca ccacgcggga gcgtcacggc cgcaacctac cggaaccggt
     1021 accctcggcg acatggtcga cgtgcagttg cggcgtcccg tcgcgccgtc ggacgtcgac
     1081 gtgctccgac gcgtcgcgga cgacgcggga cgtgccgccg gccatgcggt cgtcggtgac
     1141 actgtctggc gcgacctcgc tgatccgtcg cccgacaccg cgatcatcgt ggcgcgcgac
     1201 ggggaatcga ccgtcggcgc gatgcacatc ggatccaacg actcgcgcac cacgccccac
     1261 accaccctgt cggtcgcggt cgcgcccggc gcgccccacg gcgaggtcgt cgccgcgatg
     1321 acgacgcccg cgcttgccga ccggcgagcg cacggcggcg ggcacgtcga gctctgggtc
     1381 ctcggcacgg accggtcctg ggacagccgg gcccgccgtc tcgggctcga ccccgcccgc
     1441 gagctgcgcc agctgcgggt ccggctcccg gtcacggagc cggtgacttg gccgccgggg
     1501 gtgacggtcc gccgcttcga ggtcggccgc gacgaggccg cgtgggtcgc cgtcaacaac
     1561 cgggccttcg cccgagaccc cgaccagggc ggctgggtgc aggcgaccct gcgtcgccgc
     1621 gagcaggagg agtggttcga tgcggccggg ttcctcctcg ccgaagacga ccgcggcctc
     1681 gccgggttct gctggacgaa actgcacccg gcggcgccac cgatcgagcc ggtcgcgctc
     1741 ggtgaggtct acgtcatcgg cgtcgaaccg ggccgtcaag gctccgggct cggccgcagc
     1801 ctggtgctcg ccggcctggc cgacgtgcac gaccggcgtg gcgcaccggt cgggatgctg
     1861 ttcgtcgaca ccacgaacga cgcggcgctg cgactttacg aatcgatcgg gtttaccgtg
     1921 acacgcgtgg atcgggcgta cgcatgcgag ctctgacccg ctacggcgcg tcccgagccg
     1981 agctggcctc gctgctggac gacgagcccc ggtaccgggt cgaccaggtg tgggacgccc
     2041 tgtggaggcg gcgggtggcg ctggagtccg ccaccgagct ccccggggca ctccggaccc
     2101 ggctggccga cctgctcccc cttgccttgg ctcccgtcac cgagctcgtg tcggccgacg
     2161 agctcacccg caagtggctc tgggccgccg gggacgccca ggtcgagacg gtcctcatgc
     2221 gctccccccg acgagccagc gtgtgcgtct cctcccaggc cggctgtgcg atgggctgca
     2281 ccttctgcgc gaccggccaa gccggattcg accgtcacct ccgggccgat gagatcgtcg
     2341 agcaggtgct gcgagccgag cacgcgtcac cgcaacgggt caccaacgtc gtctacatgg
     2401 ggatggggga gccgctcgcc aactacgacg aggtttgggc ctcagtcgtg acgctgaaag
     2461 aggcgttagg tctctcggcg cggcggctca cgatcagcac cgtcggtgtc gtccccggca
     2521 tgcggcgcct cgccaccgaa ggtctcccgg tcacgttggc agtgtcgctg cacgcccccg
     2581 acgatgcgct gcgagccacc ctggtgcccc tcaaccaccg gtacccgatc gctgaggtgc
     2641 tcgacgccgc cgccgacgtg gccggcgcca gcggccgccg agtcacgttc gagtacgccg
     2701 ggatcgccgg cgtgaacgac cagccggccc acgccgaggc cctcgcccgg ctgctcggct
     2761 cctggcccgg tgtcggaggc tcccacgtga acctcatccc cctcaacgcg accgccgggt
     2821 tccccggtcg cgcctcggcg ctccccgcgc tgcacgactt cgcccgccgc ctccgggccc
     2881 gaggcgtcac cgccaccgtc cgccggaccc gaggcgccga catcgacgcc gcgtgcgggc
     2941 agctccgccg acggaccctg atcccgccgg agcggtccgg ttcgaccaga atgggcccgt
     3001 gcgccagtac cggttcctga accaggctca gccccagacc ctcttcatcg ccaccctgtt
     3061 ctgctatatc gacgccgtct tcgggctgct cggtggggtg gtcaccatct cggtgctgtt
     3121 ggccctcctg accatcctcg gcctcgcgct cggcggggtc ggga
//
LOCUS       DATCCH010000104         2363 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694 k141_5642,
            whole genome shotgun sequence.
ACCESSION   DATCCH010000104 DATCCH010000000
VERSION     DATCCH010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5642"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="VIJ44_02120"
     CDS             <1..332
                     /locus_tag="VIJ44_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164726.1"
                     /translation="LDLPPVLVHHPDGTRTEYSNLDASWRSGFLHSSHHFVDALLAGR
                     TDPEMSGDLAVKTLQLCFAVYQASNERRPVDPATITASVSPPWWPPDLSVTPRFMESY
                     GHEPPRR"
     gene            633..1832
                     /locus_tag="VIJ44_02125"
     CDS             633..1832
                     /locus_tag="VIJ44_02125"
                     /inference="COORDINATES: protein motif:HMM:NF015541.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HEY5164727.1"
                     /translation="MKREHNWSRLGAALLGLVVVVVAVAAAAANGTPAGKLPRQAPAG
                     DAFYRPQGLRSGRAGDVIWTAPIIAPAGSRGWKILYHSQAIDGHDIAVSGVVIVPTSK
                     VPSGGRPVISWAHGTHGLADACAPSKALDIAYRIPGIKDFIKQGDIVVATDYEGLGTP
                     GEHPYLVGESEARGVLDIVRAVRQMPGSGASATTLVYGHSQGGQAALFAAQIASSYAP
                     ELHLLGVTAVAPVADVTAMVPMATTVPALLGYDVMTAVGFRAAYPNVDLSTVLGPQAL
                     ANLNVVDQRCSDDVVDYFAQYPPSQVVAHNPLDVPVLAQLLQQSTAGDVHTAVPQFIV
                     QGDLDALVPKTVTDRYVRRACGQGDQLLYRVYPGQDHITARDVSVPDVEAWLAARLAG
                     QPAPSSC"
     gene            complement(1860..>2363)
                     /locus_tag="VIJ44_02130"
     CDS             complement(1860..>2363)
                     /locus_tag="VIJ44_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164728.1"
                     /translation="NRAIINRDRTAVIGVGGTRLGAIAAISVLGAWAAPRLSKPVLVA
                     TTGLVAAAALRRGRLRRLVWLTSTLRRESPDAILVGEFAAREPGAGVAFAVQALDAIG
                     TRVTLALTVQGARDDRRARSLVRLYERRFGFETVARHSVGSDDVVLMVRRASVTTLSR
                     TAVPTGS"
BASE COUNT          344 a          867 c          794 g          358 t
ORIGIN      
        1 tgctcgacct cccgccggtc ctggtccacc atccggacgg gacccgcacc gagtactcca
       61 acctcgacgc cagctggcgc tccggcttcc tgcactcctc gcatcatttc gtcgacgcgc
      121 tcctcgcggg ccgaaccgac cccgagatga gcggcgacct cgcggtcaag acgctccagc
      181 tgtgcttcgc ggtgtatcag gcgagcaacg agcgtcgccc ggtggacccc gcgacgatca
      241 ccgcatcggt gtcgccgccg tggtggcctc ccgacctctc ggtcacccca cggttcatgg
      301 agagctacgg ccacgaacca ccgcgccgct gacctcggcc cacgcgaggc gccgtgacat
      361 gcctccccca aaccaccggt ttgtgattta gtgggtcgtg gccctcgggt cggggacgcc
      421 ggtcgccgcg agcaccgccg gctggttcct ccgctcggcc gtgcgcttga tggactccct
      481 ccttggtctc ccacccctcg ttctccttcc cccgggcggg tcgcggcctc ctggggaccg
      541 gttcaagacc tcccccggat cggtcgatga acacgactgg tcctcgggtc ggtttcgacc
      601 gccggggccg tcggtcggct gggagcgatg cgatgaagcg tgagcacaac tggtcccgtc
      661 tcggggcggc gctgttgggc ctcgtcgtgg tggtcgtcgc ggtggccgca gccgcggcga
      721 acggaacgcc cgcgggcaag ctgccccgcc aagcaccggc cggcgacgcc ttctatcgcc
      781 cccagggtct gcgttcgggg cgtgccggcg acgtgatctg gacggcgccg atcatcgccc
      841 cggctggctc gcgaggctgg aagatcctgt atcactccca agccatcgac gggcacgaca
      901 tcgccgtgtc cggggtcgtg atcgtcccga cgtcgaaggt cccgtcgggc ggacggcccg
      961 tcatcagctg ggcgcacggc acgcacgggc tcgcggacgc gtgcgcgccg tcgaaggcgt
     1021 tggacatcgc gtaccggatc ccgggcatca aggacttcat caagcagggc gacatcgtcg
     1081 tcgcgaccga ctacgaaggg ctcgggacgc ccggtgagca tccgtatctc gtcggtgaga
     1141 gcgaggcccg cggcgtgctc gacatcgttc gggcggtccg ccaaatgccc ggcagtgggg
     1201 cgagcgcgac aacgctcgtc tatgggcact cccagggtgg ccaggcggct ctctttgccg
     1261 cacagatcgc gtcgagctac gcgcctgagc tgcacctgct cggcgtcaca gcagtcgcac
     1321 cggtcgccga tgtgacggcg atggtcccga tggccacaac ggtccccgcg ctcctcggat
     1381 acgacgtcat gaccgcggtg gggttccggg ccgcgtaccc gaacgtggac ctgagcaccg
     1441 tcctcggtcc gcaggccctc gccaacctca acgtcgtcga tcaacgctgc agtgacgatg
     1501 tcgtcgacta cttcgcccag tacccgccga gccaagtcgt cgcgcacaac ccgctcgacg
     1561 tccccgtcct cgcgcagctc ctccagcagt ccaccgccgg tgacgtgcac accgccgtgc
     1621 cccagttcat cgtgcagggc gacctcgacg ccctggtgcc gaagacggtc accgaccgtt
     1681 acgtccggcg ggcatgcggc caaggcgacc agctcctcta ccgcgtctac cccggacagg
     1741 atcacatcac cgcccgtgac gtgtccgtcc ctgacgtcga ggcgtggctc gccgccagat
     1801 tggccggcca gcccgcccca tcatcctgtt agtgccggcg gggggaccac cgggcttctt
     1861 caggagcccg tggggaccgc agtccgcgac agcgtcgtca ccgacgctcg gcgcaccatg
     1921 agcacgacgt cgtcggagcc aaccgagtgg cgggccacgg tctcgaaccc gaagcgccgc
     1981 tcgtagaggc gaacgaggga acgggcgcgg cggtcgtcgc gggcgccttg caccgtcaag
     2041 gccagggtga cgcgggtccc gatcgcgtcg agggcctgga cggcgaacgc gacgccggct
     2101 ccgggctcgc gtgcggcgaa ctcgcccacc agaatcgcgt ctggggattc tctccgcagt
     2161 gtggaggtca accacaccag tcggcggagc cggcctcgac gcagcgccgc cgcggcgacg
     2221 agaccggtgg tggcgacgag aaccggtttg gagaggcgcg gcgcggccca ggcgccgagc
     2281 acgctaatgg cggcgatggc gcccagccgg gtcccgccga cgccgatgac ggcggtgcga
     2341 tcccgattga tgatcgcccg att
//
LOCUS       DATCCH010000105         2433 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_301682, whole genome shotgun sequence.
ACCESSION   DATCCH010000105 DATCCH010000000
VERSION     DATCCH010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301682"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="VIJ44_02135"
     CDS             complement(<1..694)
                     /locus_tag="VIJ44_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEY5164729.1"
                     /translation="MTPRKRAALLLAVLGPGVIAANAGNDAGGIATYASAGSQFVYRM
                     LFLMVLVTIALVVVQEMSARLGAYSGEGLVSLIREQFSLRTATFAVVCLVVANVGLVV
                     SEFAGIGAAFEIFGVSRYIAVPLAAVGIWAVVIFGSYRYAERVFLLLSLAFLAYPIAV
                     FLGNPDWGQVATNTLWPHFIASHSFIVLAVALVGTTITPYMQLYQAAAVSDRGIGPDD
                     YPRERIDSVGGAI"
     gene            complement(712..2049)
                     /locus_tag="VIJ44_02140"
     CDS             complement(712..2049)
                     /locus_tag="VIJ44_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY5164730.1"
                     /translation="MARRPPFPSPAALRLRRDRRTERLLANRAVVDALTSVAGLEGRP
                     VRITGGSEVGRLVDLVARWDGEPYPPVTGLVIRVGRRRSFIPGEQLAELGRSEVTLRS
                     ATFSLRDFERRPGEVLLAGDVLDHQLVDVDGVRVVRASDLYLAQVGDGWRLVGADVGY
                     HTLLRRLGPRRWRARPTPERVIDWAAIQPFGVDPGPLRLRRTNQSLSRLRPGELADLL
                     EELGRPERTELLEALGPDSAADALEEMEAEELRALLRDLPTRRAAELIGAMEPDEAAD
                     ALRDLDPDELDEVFAAMTPDVAMRLRPLLAYPERTAGGLMTTRLVLAHRRERVGAVRD
                     RLAANTGEHGDLDAVVVVDDGGRLLDDLRLVELLVADADEIFEHLVATSEPVTVNSDA
                     SLAEVVERLTESRRTSVIVTDDDHHPIGRILADDVIDALVPSRGRHRVHVRFP"
BASE COUNT          407 a          823 c          889 g          314 t
ORIGIN      
        1 agatggcccc gccaacgctg tcgatgcgct cgcggggata gtcgtcgggc ccgattcccc
       61 ggtcgctgac cgccgccgct tggtacagct gcatgtacgg ggtgatcgtg gtgccaacca
      121 acgccaccgc gaggacgatg aagctgtggc tggcgatgaa atgcggccac aaggtgttcg
      181 tcgcgacctg accccagtcg gggttcccga ggaacaccgc gatcgggtac gcgaggaacg
      241 ccagcgacag cagcaagaac acccgctcgg cgtaccggta ggagccgaag atcacgaccg
      301 cccagatccc gaccgcggcg agcggcaccg cgatgtagcg cgagacgccg aagatctcga
      361 acgcggcgcc gatccccgcg aactccgaga cgacaagacc gacgttggcg acgacgaggc
      421 acacgaccgc gaacgtggcg gttcgtagcg agaactgctc ccggatcagc gagaccaggc
      481 cctcacccga gtacgcaccg agccgggccg acatctcttg gaccacgacc agggcgatgg
      541 tgaccagcac catcaagaac agcatccggt acacgaactg tgacccggct gaggcgtacg
      601 tcgcgatccc accggcgtcg ttgccggcgt tcgccgcgat caccccggga cccaacacgg
      661 cgaggagcaa cgcggcccgc tttcgcggcg tcatccgggc gaagggtcgg atcatgggaa
      721 tcggacatgg acgcggtggc gaccgcggga cgggacgagg gcgtcgatga cgtcgtcggc
      781 gaggatccga ccgatcggat ggtgatcgtc gtcggtgacg atcaccgagg tgcgccgcga
      841 ctcggtgagt cgttcgacga cctccgcgag cgacgcgtcg ctgttcaccg tgaccggctc
      901 cgaggtcgcg acgagatgct cgaagatctc gtcggcgtcg gcgacgagca gctcgacgag
      961 acgcaggtcg tcgaggaggc ggccaccatc gtcgacgacg acgaccgcgt cgaggtcacc
     1021 gtgctcaccg gtgttggcgg cgaggcggtc gcggacggca ccgacccgct cccgacggtg
     1081 cgcgaggaca aggcgggtgg tcatgagccc accggcggtg cgctcggggt aggcgagcag
     1141 cggacggagt cgcatcgcca catccggggt catcgccgcg aagacctcgt cgagttcgtc
     1201 aggatcgagg tcacggagcg cgtcggcggc ttcgtcgggt tccatcgcgc cgatcagctc
     1261 cgcggcgcgt cgggtgggca ggtcgcgcag cagcgcccgg agctcttcgg cctccatctc
     1321 ctccagcgcg tcggcggccg aatcgggtcc gagcgcttcg aggagctcgg tccgttccgg
     1381 tcggcccagc tcctcgagca ggtcggcgag ctcgccgggg cgcagccgcg acagggactg
     1441 gttggtgcgg cgcagccgga gcggtccggg gtcgacaccg aagggctgga tcgcggccca
     1501 gtcgatcacc cgctctgggg tcgggcgggc ccgccaccgt cggggaccca gtcgtcgcag
     1561 cagcgtgtgg tatccgacgt cggcgccgac cagtcgccac ccgtcgccca cctgggccag
     1621 gtacaggtca gaggcccgca ccacacgcac cccgtcgacg tcgacgagct ggtggtcgag
     1681 gacgtccccg gcgaggagca cctccccggg gcggcgttcg aaatcccgga ggctgaacgt
     1741 ggcggaccgc agggtgacct cggagcggcc cagctcggcg agctgctcgc cggggatgaa
     1801 cgaccgtcgc cgtccgaccc ggatgacgag gccggtgaca ggcgggtacg gctcgccgtc
     1861 ccagcgggcc acgaggtcga cgagacgtcc aacctcggag cccccggtga tgcgaaccgg
     1921 ccggccttcg aggcccgcga cggaagtgag cgcgtcgacg acagcgcggt tcgcgaggag
     1981 acgttcggtg cgacggtcgc ggcggagccg cagcgcggca gggctgggga acggcggacg
     2041 gcgggccatg gggtcctccg ggggttccgg cgggcagggc gagcagatcg agcccgcgcc
     2101 gccggagacg ccgtcgcgaa ggagcgacta gcgcaaggtg ttccggcggg ggacgggccg
     2161 acggctggga ctcgggggtt cgcttcgtcg tcgcatccgc ctcacctcct cggttcccgc
     2221 gctccaggcg cgatcagcac caccagccac gacgcatgtt accgctgtgg gttcatcacc
     2281 gggccgtttc gcaccgcaat ccggattccg tgtcgatccg tatgaccccg agagcgccgg
     2341 ggaagcactc accaccagac caaggagaag gaaagccccg atgcgtctca cggggacctg
     2401 gacccggatc atcggcgccg tcaccgcggt gct
//
LOCUS       DATCCH010000106         3252 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_379386, whole genome shotgun sequence.
ACCESSION   DATCCH010000106 DATCCH010000000
VERSION     DATCCH010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379386"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VIJ44_02145"
     CDS             complement(<1..278)
                     /locus_tag="VIJ44_02145"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY5164731.1"
                     /translation="MTADDNKAIVRRLWAALYDRHDFDEVGALFASDGYYCDMPHPDQ
                     GATGPAEVAARLRLGLAKLTDHTHDIERITAEGDTVVTEHTEHWFFPTG"
     gene            complement(451..1524)
                     /locus_tag="VIJ44_02150"
     CDS             complement(451..1524)
                     /locus_tag="VIJ44_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164732.1"
                     /translation="MSIRWPRPPTEHRTAGTAARPVPALVVLAVGAALVGFGFRSPTP
                     AATPTISWSELHNPLLAYPDRALKDPALVWSDHRWVALFSQVGVDAHWTLGVATSVDL
                     RRWTAPTTVAHDPAVEGDASPDVVRAPDGTFVVTDQSFVHDVDGSEAKLYYRTTRDFR
                     SFSAPRPLGRSLPPGPSDRMIDASLAWTPAGLLLGYKVGGADSPQAFEIARSTTGSLD
                     GPWALIGRPQITVIGDTIENYQFLALQGRWQLLATSNRFDRPYLFQLTGDPHDPRGWL
                     RWSAGRQLQVPQEPWNRGRGTTGVSYEHANGAFLVNRGQVAGHFYLIFADASELASFG
                     GSGHDELALARSTDLRHWSVPPH"
     gene            complement(1595..2107)
                     /locus_tag="VIJ44_02155"
     CDS             complement(1595..2107)
                     /locus_tag="VIJ44_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012087349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY5164733.1"
                     /translation="MTKARWLDEHERQTWLIFVYANQLFFEQIERDLQRDADMPFAYY
                     ETLVILSESPGRSMRMSELAEASLFSRSRLSHAIARLEAAGWVRRESCPSDRRGSFAV
                     LTDEGYAVLEAAAPKHVESVRAHLFDQLSPSQLDALRDISETLTRHLIPSLGVTPPPV
                     LACPDPVASG"
     gene            complement(2251..2820)
                     /locus_tag="VIJ44_02160"
     CDS             complement(2251..2820)
                     /locus_tag="VIJ44_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018658335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEY5164734.1"
                     /translation="MTVVVTEIPGYIAGTWTIDPSHSEVSFTIRHLMVSKVRGRFTGF
                     EGQVVTGADPLQSSVRATVDLASIDTANADRDTHVRSTDFFDVARHPTMTYQSTGVRF
                     DGDDLVVDGELSLHGVTRSVSLKLEVNGFQPSTPFGDTRVGFSATGELNRSDFGITFN
                     MPLDSGGIALGEKIRLTIEIEAILDSPAA"
     gene            2995..>3252
                     /locus_tag="VIJ44_02165"
     CDS             2995..>3252
                     /locus_tag="VIJ44_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5164735.1"
                     /translation="MEVKELGHLVLYVRDLEVSAAFYRDVLGFRPITPTIPGMRAAAF
                     SSGRTHHELLLIEVGADASPVPRGRHLGLYHFGLKVGDSDDE"
BASE COUNT          489 a          932 c         1311 g          520 t
ORIGIN      
        1 ccggtcggga agaaccagtg ctcggtgtgc tcggtgacca ccgtgtcccc ttcggcggtg
       61 atgcgctcga tgtcatgggt gtgatcggtc agcttggcga ggccgagccg gagccgggcc
      121 gccacctcgg ccgggccggt ggcgccctgg tcggggtggg gcatgtcgca gtagtagccg
      181 tcggaggcga agagcgcccc gacttcgtcg aagtcgtggc ggtcgtagag cgcggcccac
      241 aagcgtcgca ctatcgcctt gttgtcgtct gcggtcatgg cggctccgct cgtcccgatc
      301 gacgttatcg gcgcggaccg ccagcgcggt gggccgaggc gtcgtcgacg gatggcgagc
      361 gcgaagaggt gtcggctgtg agcgatgggg tcggcgatca cgtcgaggcg ggcgctgccc
      421 cgcgatcgtg acgcggggga ggggtactcg tcagtggggt gggacggacc agtggcgcag
      481 gtcggtgctg cgggccaagg cgagctcatc gtggcctgat ccgccgaagc tggcgagctc
      541 cgacgcgtcg gcgaagatca gatagaagtg gccggcgacc tggccgcggt tgacgaggaa
      601 cgcgccgttg gcgtgctcgt aggaaacgcc ggtggttccg cggcctcgat tccacggctc
      661 ctgggggacc tggagctgtc gcccggctga ccagcgcaac cagccgcgcg ggtcgtgggg
      721 gtcgccggtg agctggaaca ggtagggccg gtcgaagcgg ttgctcgtgg cgagcagctg
      781 ccagcgaccc tgtagcgcga ggaactggta gttctcgatg gtgtcgccga tcacggtgat
      841 ctggggtcgc ccgatgagcg cccagggacc gtcgagggat ccggtggtgg aacgggcgat
      901 ctcgaacgct tggggactgt ctgcgccgcc gaccttgtat ccgaggagca gcccggccgg
      961 ggtccacgcc agcgaggcgt cgatcatccg gtcgctgggt ccggggggga gtgagcgtcc
     1021 cagcggacgc ggggcggaga agctgcggaa gtcccgggtg gtgcggtagt agagctttgc
     1081 ttcgctgccg tcgacgtcat ggacgaacga ctggtcggtg acgacgaagg tcccgtcggg
     1141 ggcccggacg acatcgggcg acgcgtcacc ttcgaccgcc gggtcgtggg ccacggtcgt
     1201 cggggctgtc cagcggcgga ggtcgacgct cgtcgcgacg ccgagggtcc agtgggcgtc
     1261 gacgcccacc tggctgaaca gggcgaccca acggtggtcg gaccagacca gggcgggatc
     1321 cttcagcgcc cggtcgggat acgcgaggag cgggttgtgc agctccgacc acgagatggt
     1381 cggtgtcgcg gctggggttg gcgaccggaa cccgaagccg acgagcgcgg cgccgaccgc
     1441 gaggacgacc agcgcgggga cgggccgagc cgccgtgccg gcggtccggt gctcggtcgg
     1501 cggtctcggc cacctgatgg acacgcggag cacggtacga gaccgcggcg ccaggcgaga
     1561 tggtcggcgc gcgggtgggt cgaggtgggg tgggtcagcc cgaggcgacg ggatcggggc
     1621 aggccagcac cggtggtggt gttacgccga gtgaggggat gaggtgtcgg gtgagggtct
     1681 cgctgatgtc gcggagcgcg tcgagctggg acggtgagag ctggtcgaag aggtgggcgc
     1741 ggacgctctc gacatgcttg ggcgcggcgg cttccagcac cgcgtagccc tcgtcggtga
     1801 ggaccgcgaa ggagccgcgg cggtcgctgg ggcagctctc ccggcggacc catccggcgg
     1861 cttcgaggcg agcgatggcg tgggagagcc ggctccgcga gaagagcgac gcttcggcga
     1921 gctcgctcat gcgcatggat cggccggggg attcggacag gatcacgagg gtctcgtagt
     1981 acgcgaacgg catgtcggcg tcgcgttgga ggtctcgttc gatctgctcg aagaacagtt
     2041 ggttggcgta cacgaagatg agccaggtct gtcgttcgtg ctcgtcgagc cagcgggctt
     2101 tcgtcattgg gtcatcatac gacaatcttg ttgaccgttc aatcatcggg ggcgtgggct
     2161 gggggtgact cctcgcgcct cggcgacccg ctccgtgccc acgaggacgg gagcaggtcg
     2221 ccgggtggtg cgctcggcgg ggcgcgccgg tcaggcggcg gggctgtcga ggatggcctc
     2281 gatctcgatg gtgaggcgga tcttctcgcc gagggcgata ccgccactgt cgaggggcat
     2341 gttgaacgtg atgccgaagt cgctgcggtt gagctcgccg gtggccgaga agccgactcg
     2401 ggtgtccccg aacggggtgc tgggctggaa gccgttcacc tcgagcttga gggacaccga
     2461 gcgggtgacg ccgtggaggg acagctcccc gtcgacgacg aggtcgtcgc cgtcgaagcg
     2521 aacgccggtc gattggtagg tcatggtcgg gtggcgtgcg acgtcgaaga agtcggtcga
     2581 gcggacgtgg gtgtcgcggt cggcgttggc ggtgtcgatc gacgccaggt caacggtggc
     2641 gcggaccgag gactggaggg gatcggcgcc ggtgacgacc tggccctcga agccggtgaa
     2701 gcggcctcga accttgctca ccatgaggtg gcggatcgtg aaggagacct cggagtggga
     2761 cgggtcgatg gtccaggtgc cggcgatgta gccggggatt tcggtgacga cgacggtcat
     2821 ggattcgctc ctctatttga gttctaaact gttgaacacg agagtatcat gagataattg
     2881 agaggtcaac cgttgagggc gcgaccgctc atcgcccagg cggcggtccg tcgggcgacg
     2941 agaattgccg ggggagcgac ccggggcggg taccgcagaa cgggaggagg atcgatggag
     3001 gtcaaggagc tcggacacct ggtcctgtac gtccgggacc tcgaagtgtc ggcggcgttc
     3061 tatcgggacg tcctcggctt ccggccgatc acgccgacca ttccggggat gagggcggca
     3121 gccttctcga gcgggcgcac gcaccatgag ctgctcctca tcgaggtcgg cgccgacgcg
     3181 tcgccggtcc cccggggacg acacctgggc ctgtaccact tcgggctcaa agtcggggac
     3241 agcgacgacg aa
//
LOCUS       DATCCH010000107         2495 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694 k141_5879,
            whole genome shotgun sequence.
ACCESSION   DATCCH010000107 DATCCH010000000
VERSION     DATCCH010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5879"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="VIJ44_02170"
     CDS             <1..398
                     /locus_tag="VIJ44_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164736.1"
                     /translation="PLADAVADAPDLPRRLETALTFAHRAVRDHAVLQKTLTNEPGAL
                     LPLMTVEQHRVIRYISDYLRPVLAAEQAAGRLRPGVDVDHAADFLARMGLSLIGSPGR
                     WDLEDPDAVRTLVRTELLAGILRPEPAPE"
     gene            488..919
                     /locus_tag="VIJ44_02175"
     CDS             488..919
                     /locus_tag="VIJ44_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164737.1"
                     /translation="MGMFKDINKMKKQGQEMQKAQGKRTGMIGMLRDMPSNISQASSA
                     VDDAMALQADIQQQQALLTTGTQGKATIKNFTDTGTLVNFNPQVVLDLEVTCPGQDPY
                     AAKLTTAVPQVYLAQLQPGASIGVRVDPSDPSSIALDWAAG"
     gene            1061..1693
                     /locus_tag="VIJ44_02180"
     CDS             1061..1693
                     /locus_tag="VIJ44_02180"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY5164738.1"
                     /translation="MSASLTDREQRAPARPEVEARAVAALLSCVARQGLRKTTLDDVA
                     REAGCGRATLYRYFASKQALLAAAGHTEAIRIVAALRDAAAAADTLEDAVVALLVTAG
                     VELGAHPALRFVADFEPDWLLPHLAFAGGDRFLAAAADALTPALDRFVGSDHAGRAAE
                     WIARVGLCLLCSPVSPVALNDEASLRAYTREFIVPALNPLSVRSPASTRR"
     gene            1699..>2495
                     /locus_tag="VIJ44_02185"
     CDS             1699..>2495
                     /locus_tag="VIJ44_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEY5164739.1"
                     /translation="MSSTEELIGRADLNDIEAILAVTNTDVDEVTHAIRANADAIFTW
                     DYEQSRQPLKKLYEKAKTSQWNSIDLPWDTDVDQEAVAAANQVQNGYGDNRIDLAGTP
                     FEHWGDKEWLDFNIESQNWTLSQFMHGEQGALICTAQIVETVPWIDAKYYASTQVMDE
                     ARHVEVFSRYLSEKLSGFYPINAHLKMLLDDIIADSRWDMTYLGMQIMVEGLALAAFG
                     FMHQMTTEPLLKQLLRYVMSDEARHVAFGVLSLKEYYAELSAAEIRER"
BASE COUNT          427 a          975 c          720 g          373 t
ORIGIN      
        1 cccccctcgc cgacgccgtc gccgacgccc ccgatctccc ccgacggctc gagaccgccc
       61 tcacgttcgc tcaccgggcg gtccgggacc acgccgtcct ccagaagacc ctcaccaacg
      121 aacccggcgc gctccttccc ctcatgaccg tcgagcagca ccgcgtcatc cgctacatca
      181 gcgactacct ccggccggtc ctcgccgccg agcaagctgc cggccggctc cggcccggcg
      241 tcgacgtcga ccacgccgcc gacttcctgg cccgcatggg cctctccctg atcgggagcc
      301 ccggccgctg ggacctcgag gaccccgacg ccgtccgaac cctcgtccgc accgagctcc
      361 tcgccgggat tctgcgcccc gaacccgcac ctgagtagga cttactcaag actcgcggtc
      421 tcaaccgcga gacgatggcg ccagtcagac cggcgtccgc cggtccaccg atctcgagga
      481 gccacccatg gggatgttca aggacatcaa caagatgaag aagcagggcc aggagatgca
      541 aaaggcccag ggaaagcgca ccggcatgat cggcatgctg cgcgatatgc cgagcaacat
      601 cagccaggcc agcagcgccg tcgacgacgc gatggccctc caggccgaca tccagcagca
      661 gcaggcgctg ctcaccaccg gcacccaggg caaggccacg atcaagaact tcaccgacac
      721 cggcaccctg gtgaacttca acccacaagt cgtcctcgac ctcgaggtca cctgccccgg
      781 ccaggacccc tacgccgcca agctgaccac cgcggtcccg caggtgtacc tggctcagct
      841 ccagcccggc gccagcattg gtgtccgggt cgacccgtcg gacccgtcgt cgatcgccct
      901 cgactgggcc gcgggctgac ccgattgctc gaacgaccgt acgggggcgg cctgcgggcc
      961 gcccccgacg cgtcgccccg gctcggcccc ggctgagacc tcccctgatc catcccgggg
     1021 tcgttgacgc tgagacgaaa tcagtcctat tgtctcaggg atgagcgcgt ctctcaccga
     1081 ccgggagcaa cgcgcgcccg ctcgccccga ggtcgaggcg cgcgctgtcg ccgccctttt
     1141 gagctgcgtc gctcgccagg gtctgcgcaa gaccaccctc gacgacgtcg cccgcgaggc
     1201 cggctgtggc cgagccaccc tgtaccggta cttcgccagc aagcaggcgc tcctcgccgc
     1261 cgctggccac accgaggcca tccgaatcgt cgccgcgctg cgcgacgccg cggccgcggc
     1321 ggacacgctc gaagatgccg tcgttgcctt gctggtcacc gcgggggtcg agctcggcgc
     1381 ccatcccgcg ttgcggttcg tcgctgactt cgaacccgac tggctcctcc cgcacctcgc
     1441 gttcgcgggt ggggaccgct tcctcgctgc cgccgccgac gcgctcaccc ccgccctgga
     1501 ccgtttcgtg ggctccgacc acgcggggcg cgccgccgag tggatcgcac gagtcgggct
     1561 ctgcctcctg tgctcccccg tgtcgcctgt cgcgctcaac gacgaagctt cgctgcgtgc
     1621 ctacacccgc gagttcatcg tccccgccct caacccgctt tccgtccgtt cgccagcttc
     1681 gacccggagg tgactgccat gtccagcact gaagaactca tcggtcgtgc cgacctgaac
     1741 gacatcgagg ccatcctcgc cgtcaccaac accgacgtcg acgaggtcac ccacgccatc
     1801 cgcgccaacg ccgatgccat tttcacctgg gattacgagc agtcccgcca accgctcaag
     1861 aagctgtatg agaaggccaa gacctcccag tggaactcga tcgacctgcc ctgggacacc
     1921 gacgtcgacc aggaagccgt cgcggccgcc aaccaggtcc aaaacggcta tggcgacaac
     1981 cgcatcgatc tcgccggcac ccccttcgag cactggggtg acaaggaatg gctggacttc
     2041 aacatcgaat cccagaactg gaccctgtcc cagttcatgc acggcgagca gggcgccctg
     2101 atctgcaccg cgcagatcgt cgagaccgtc ccttggattg acgccaagta ctacgcgtcg
     2161 acccaggtga tggacgaagc ccgccacgtc gaggtgttca gccgctacct ctcggagaag
     2221 ctgtcgggct tctacccgat caacgcgcac ctcaaaatgc tgctcgacga catcatcgcc
     2281 gacagccgct gggacatgac ctacctcggt atgcagatca tggtcgaagg cctcgccctc
     2341 gccgcgttcg ggttcatgca ccagatgacc accgagccgc tcctcaagca gctgctccgc
     2401 tacgtgatga gcgacgaggc ccgccacgtc gccttcggcg tcttgtccct caaggagtac
     2461 tacgccgagc tgtccgcggc cgagatccgc gagcg
//
LOCUS       DATCCH010000108         2242 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_260947, whole genome shotgun sequence.
ACCESSION   DATCCH010000108 DATCCH010000000
VERSION     DATCCH010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260947"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="VIJ44_02190"
     CDS             <1..483
                     /locus_tag="VIJ44_02190"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEY5164740.1"
                     /translation="GGAGVTDNEALAAQVRLLRGHGLARDYEHVVTSDNARLSEVEAA
                     ALRVGLRRLVSYNDRRRMIARHYRDAAPGLFWQAPHARHVYHLCVARVPDRESFRDRL
                     PFLTGVHYPRALTQQPAYGGYTRFSCPESEAWAAECVSFPCFPELTDDEIEVVCRALR
                     "
     gene            465..1217
                     /locus_tag="VIJ44_02195"
     CDS             465..1217
                     /locus_tag="VIJ44_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY5164741.1"
                     /translation="MPRTPMNPAVESISAFFPCFDDEPTIGSMVNLAFATIEKVGADG
                     DVIVIDDGSIDGSPYVLKELADTHPRLRIVTHEHNRGYGGALLSGFAAATEQWVFYTD
                     GDAQFDPSELELLVRAARDDIDVVQGYKIRRADSLTRRVIGRVYHRFVALAFGLTIRD
                     TDCDFRLIRLAALQRVELVHTSGVICVELVRKLQDSGAHFVEVPVHHYRRVNGQSQFF
                     RPAQVVSSLVDLARLWFRLVVFGRGRRQFSKV"
     gene            complement(1206..1946)
                     /locus_tag="VIJ44_02200"
     CDS             complement(1206..1946)
                     /locus_tag="VIJ44_02200"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY5164742.1"
                     /translation="MELDEYTRIAAVEDDHWWYRNTRAVMADLLGPWLGANQTILDAG
                     CGPGGNGAWLARHGEVAALDLAPEGLRFVRDRRPELNPVRASVTALPFPDGRFDVAVA
                     VTVVYSVPDDHLAVVELARVLRPGGALLLIEPAFRALRRAHDTTVHSVHRYRRAELAA
                     LASEAGLAVRRATYAYSFLTPPAAALSVADRLRPRADRAAGSDVERRWLDGLFEPLAR
                     LERRRLRHADLPFGTSAIVLATSPTSDL"
     gene            complement(1985..>2242)
                     /locus_tag="VIJ44_02205"
     CDS             complement(1985..>2242)
                     /locus_tag="VIJ44_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164743.1"
                     /translation="RQHWRPLAQAPVTDPAQLYGRHVVRLGLRADQLTVAVDGDTVLD
                     VPALSRATVDLGREPCRGDRVGVQAWSTTEVTIDSFRVASL"
BASE COUNT          326 a          743 c          810 g          363 t
ORIGIN      
        1 ggcggagccg gcgtcaccga caacgaggcg ttggctgccc aggttcgcct gttgcgaggc
       61 catgggctcg ctcgcgacta cgaacacgtc gtcacctctg acaacgcccg cctctcggag
      121 gtcgaggcgg ccgcgctgcg agtggggctt cggcgtctgg tgtcctacaa cgaccggcgc
      181 cggatgatcg cgcggcacta tcgggacgcg gcgccgggct tgttctggca agcaccccac
      241 gcgcggcacg tgtaccacct ctgcgtcgca cgagtaccgg accgcgaatc gtttcgcgat
      301 cggctcccgt ttctcaccgg ggtccactac ccgcgtgcgc tcacccagca gccggcctac
      361 ggcgggtaca cacggttctc ctgcccggag tcggaggcct gggccgcgga gtgcgtatcg
      421 ttcccctgct ttcccgaact gaccgacgac gagatcgagg ttgtgtgccg cgcactccga
      481 tgaacccggc ggtcgagtcg atctccgcgt tctttccgtg cttcgacgac gagccgacga
      541 tcggctccat ggtcaacctc gcgttcgcga cgatcgagaa ggtcggcgcc gacggtgacg
      601 tcatcgtcat cgacgacggg tcaatcgacg gatcccccta cgtcctcaaa gagctcgccg
      661 acacgcaccc acggttgcgg atcgtgaccc acgagcacaa ccgggggtac ggcggggcgc
      721 tgctctctgg tttcgccgcc gcgaccgagc agtgggtctt ctacaccgac ggtgacgccc
      781 agttcgatcc gtcggagctc gagctgctgg tccgagcggc tcgtgacgac atcgatgtgg
      841 tccaggggta caagatccgc cgagctgaca gcctcacgcg gcgggtgatc gggcgggtct
      901 atcaccggtt cgtggccctg gcgttcgggc tcacgatccg tgacactgat tgtgacttcc
      961 ggttgatccg actggcggcg ttgcagcggg tcgagctggt gcacaccagc ggtgtgatct
     1021 gcgtcgagtt ggtccgcaag cttcaggact ccggggccca cttcgtggag gtgccggttc
     1081 accactatcg acgggtgaac ggccagtcgc agttcttccg tcccgcgcaa gtggtcagct
     1141 cgctggtcga cctggccagg ctgtggttcc gcctcgtcgt gttcggtcgc ggtcgccgcc
     1201 agttctcaaa ggtctgacgt cgggctcgtc gcgaggacga ttgccgacgt cccgaagggc
     1261 aggtcggcgt gtcggagtcg tcgccgctcg agccgagcca aaggctcgaa gaggccatcg
     1321 agccagcgtc gttcgacgtc agagcccgcg gcgcggtcgg cccgaggtcg gaggcggtcg
     1381 gcgacgctca gcgcggccgc gggtggcgtc aggaacgagt acgcgtaggt ggcccgccgc
     1441 acggccagcc cggcctcgga ggcgagggcc gcgagctcgg cgcggcggta ccggtggacg
     1501 ctgtgcaccg tcgtgtcgtg ggcccttcgt agagcccgga acgccggttc gatgagcagc
     1561 agcgcaccgc cgggacggag cacccgggcg agctccacga cggcgaggtg gtcgtcgggc
     1621 accgagtaca cgacggtgac cgcgacggcg acgtcgaacc gcccgtccgg gaacgggagc
     1681 gcggtgacgc tggcccggac cgggttgagc tcgggtcggc ggtcgcgcac gaagcggagc
     1741 ccttccggtg ccaggtcgag ggccgcgacc tccccgtgac gggcgagcca ggcgccgttg
     1801 ccccccggac cgcaaccggc gtcgaggatg gtttggttcg cgccgagcca cggtccgagg
     1861 aggtcggcca tgactgcacg ggtgtttcgg taccaccagt ggtcatcttc gacggcggcg
     1921 atgcgcgtgt actcgtcgag ctccaccgcg gcacggtatc ccggctggtt ggagcagccg
     1981 ggagttacag gctggcgacc cggaacgagt cgatggtgac ctcggtcgtg gaccaggcct
     2041 gcacaccgac ccggtcgccg cgacacggct cgcgaccgag gtcgacggtg gctcggctca
     2101 gggccgggac gtcgaggacc gtgtcgccgt cgacggccac ggtcagctgg tcggctcgca
     2161 acccgagtcg cacgacatgt cgaccgtaga gctgagctgg gtcggtgacc ggggcttgcg
     2221 cgagggggcg ccagtgctga cg
//
LOCUS       DATCCH010000109         2143 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_302538, whole genome shotgun sequence.
ACCESSION   DATCCH010000109 DATCCH010000000
VERSION     DATCCH010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302538"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..981)
                     /locus_tag="VIJ44_02210"
     CDS             complement(<1..981)
                     /locus_tag="VIJ44_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164744.1"
                     /translation="MTASATDWGWATDFDDVRGQVAICGIGEADHSKASGRTTKEIVG
                     QAVERALADAGLGPTDVDGLMYIPLPEQFDVAAFHEHFGTSHDMWESRAGGGMRWAAT
                     APYEAARALRDGAAHTILNTFGVAWATQRSQMVAGPGESHAHEGFKQNLEIPFGWFPQ
                     PVYFATVARRHMHEFGTTQEQLGAVAVACRRHANLTPGAVMHDRPLTMEQYLASPPIV
                     APFRKEDCCLISDGGAAYLMTTTDRARDLRTPLVEVAGVGLGNSVTGTHWAEQPAFTS
                     TPQVFAAPAAFRMAGIAPSDVDVYTCYDPFTIHTIMQIEDSGFCDKGDGGA"
     gene            complement(981..>2143)
                     /locus_tag="VIJ44_02215"
     CDS             complement(981..>2143)
                     /locus_tag="VIJ44_02215"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY5164745.1"
                     /translation="ALACLGADVIKIESVSRPDLMRYTSTVRPPEPQWFEWGPLFHGA
                     NNSKRAVTLDLTQDAGVHLLRRLLETADVLLENFTPRVMEQFGLGWGAVHDLNPALTM
                     VRLPAYGLDGPWRDRTGFAQTMEAITGMAWVTGRPDGPPLLPRGACDPLAAMHAVFAT
                     FLAVQERDRTGDGRLVEATMIEAALNMAAEQVVEYSADGRLLGRHANRGPVAAPQGVY
                     PAAGEEEWLALAIVTDAQWGALRAALGDPAWARDPALSTDAGRRAAHDVLDEELSTWC
                     ASRDARVTAERFASIGIPAAHVVDARDIAHNPQLNHRGFFEVEDHPVTGSHPLPMLPF
                     QFRGRTSGWMRGPAPTLGQHNDEILGGILGLSGDELTALRDEGVIGDRPLGA"
BASE COUNT          304 a          725 c          777 g          337 t
ORIGIN      
        1 cgcaccgccg tcccctttgt cgcagaagcc gctgtcctcg atctgcatga tcgtgtggat
       61 cgtgaatggg tcatagcacg tgtacacgtc gacgtctgag ggcgcgatgc ccgccatgcg
      121 gaacgccgcc ggcgcggcga acacctgggg tgtggacgtg aacgccggct gctcggccca
      181 gtgcgtgccg gtcaccgagt tcccgagccc gacgccggcg acctcgacga gcggcgttcg
      241 caggtcccgg gcccggtcgg tcgtcgtcat gaggtacgcc gcgccgccgt cggagatgag
      301 gcagcagtct tccttgcgga acggggccac gatcggcggc gacgccaggt actgctccat
      361 ggtgaggggt cggtcgtgca tcactgcccc cggggtcagg ttggcgtgcc ggcgacaggc
      421 gaccgcgacc gcgcccagct gctcttgcgt cgtgccgaac tcgtgcatgt ggcggcgggc
      481 caccgtcgcg aagtacaccg gctggggaaa ccagccgaag gggatctcga ggttctgctt
      541 gaacccctcg tgggcatgcg attcgccggg cccggcgacc atctgggagc gctgcgtggc
      601 ccacgccact ccgaacgtgt tgaggatggt gtgcgcggca ccgtcacgaa gtgcccgcgc
      661 ggcctcgtag ggggcggtgg cggcccagcg catgcctcct cctgcccgcg attcccacat
      721 gtcgtggctg gtgccgaagt gctcgtggaa ggcggcgacg tcgaactgct cggggagcgg
      781 gatgtacatg agcccgtcga cgtcggtcgg cccgagaccg gcgtcggcca aggcacgctc
      841 caccgcttga cccacgatct ctttggtcgt gcggcccgac gccttggagt ggtcggcttc
      901 gccgatgccg cagatcgcga cctgtccccg cacgtcgtcg aagtcggttg cccagcccca
      961 gtcggtcgcc gaggccgtca tcaggccccc aacgggcggt cgccgatgac gccctcatcg
     1021 cgcagcgcgg tgagctcgtc gccgctgagc ccgaggatgc cgccgaggat ctcgtcgttg
     1081 tgctggccca aggtgggcgc gggtccgcgc atccaaccgc tcgtgcgccc gcggaactgg
     1141 aacgggagca tcggcagcgg atgcgagcct gtgacggggt gatcctccac ctcgaagaag
     1201 cctcggtggt tgagctgcgg attgtgcgcg atgtcgcggg cgtcgaccac atgcgcggcc
     1261 gggatcccga tcgacgcgaa ccgctccgcg gtcacgcgag catctcggct ggcacaccat
     1321 gtgctgagct cctcatccag cacgtcgtgc gccgctcggc ggccggcatc ggtcgagagc
     1381 gcagggtcac gcgcccaggc ggggtctccg agcgccgctc ggagcgcacc ccactgcgcg
     1441 tcggtgacga tcgccagtgc caaccactct tcttcgccgg cggcgggata cacaccttgc
     1501 ggcgccgcga ccggcccgcg gttggcgtga cgccccagca ggcgaccgtc ggcggagtac
     1561 tcgacgacct gctcggcggc catgttcagc gcagcttcga tcatcgtcgc ttcgacgagg
     1621 cggccgtcgc cggtgcgatc gcgttcctga acggccagga aggtcgcgaa caccgcgtgc
     1681 atcgccgcga gcgggtcgca cgcgccccgg gggaggaggg gaggtccgtc aggacggcca
     1741 gtcacccagg ccatcccggt gatggcctcc atggtctggg cgaatccggt gcgatcgcgc
     1801 cacgggccgt cgaggccgta cgcagggagc cggaccatcg tcaacgccgg gttgaggtcg
     1861 tggaccgcgc cccagccgag cccgaactgc tccatgactc ggggggtgaa gttctcgagg
     1921 agcacgtcgg cggtctcgag gagacgccgg agcaggtgga cgccggcgtc ttgggtgagg
     1981 tcgagcgtga cggcccgctt gctgttgttc gcgccgtgga acagcggtcc ccactcgaac
     2041 cactgcggct cgggtggccg aacggtgctg gtgtagcgca tcaggtcggg tcgtgacacc
     2101 gattcgatct tgatgacgtc ggcgccgagg catgccagcg cgt
//
LOCUS       DATCCH010000110         3430 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_130475, whole genome shotgun sequence.
ACCESSION   DATCCH010000110 DATCCH010000000
VERSION     DATCCH010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130475"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VIJ44_02220"
     CDS             <1..223
                     /locus_tag="VIJ44_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164746.1"
                     /translation="AHGFAARAEAHGDEFTIVSECCPFGDTAQRYPHVVCALDRGMIR
                     GMLAGLYGETSPHFESSRPDGSDHCVARV"
     gene            234..1382
                     /locus_tag="VIJ44_02225"
     CDS             234..1382
                     /locus_tag="VIJ44_02225"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEY5164747.1"
                     /translation="MTRAYLDHASSSPLRPMALEAMLPYLRGNPADPGRVHSDGRVTR
                     VAIEDARAAVAALVGARPREVVFTATGTEAVNTAVWGAVTRGADRLVGRGHVVTTAVE
                     HSAVLESCRRSEVDVSIVGVDGSGRIDEAEIMAACRPETVLVSVQLANHEVGTVQSAV
                     PGIVTAVRERGVLVHVDACAAVGHVPLDFAALGADLCSITGHKLGGPKGSAALLIRRG
                     LRLPPFVVGGAQERARRAGIEDVPAIVGFGAAAAELYADDTLTTEAGTARAQTDGLLA
                     AALAVAGVTQLGDPVDRLPHLVCLAVDGVEAEPVLLGLDQHGVAVHSGSSCSSETLEP
                     SPVLAAMGVDADRSLRPSVGWSTTVADVAAFVDAFSEVVGKLRALGGG"
     gene            1390..1851
                     /locus_tag="VIJ44_02230"
     CDS             1390..1851
                     /locus_tag="VIJ44_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007357880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5164748.1"
                     /translation="MIPVRHLNHAVLYVRDATQSAAFYRDVFGFHAVAELPGAAFLRA
                     EGSTNHHDLGLFSVGPNAPGPEPGRVGLYHLAWEVGSITDLATARDTLRRVGAYVGES
                     DHGATKSIYGHDPDGNEFEVMWMVPRDEWGDHEHDAPVAPLDLDAEIARHG"
     gene            1877..2596
                     /locus_tag="VIJ44_02235"
     CDS             1877..2596
                     /locus_tag="VIJ44_02235"
                     /inference="COORDINATES: protein motif:HMM:NF014635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HEY5164749.1"
                     /translation="MTNDWPELAEGLNDALNLTAPPIAITFSDDAPPGVDPFDSPMPT
                     PTDDGRTGRVPAGCVFWIRAADRTFSTVAEDHANCSVGSLTHGFKTLDEVAGNADVAA
                     LLDAGWVTMDVVPQIPVVHDRPGTVTYGPLAETPVDPDVVLVRINGKQLMVLADALPG
                     LRIEGKPQCHIVAIAKQDGEVAASVGCALSRVRTGMPATEMTCAIPAGRLPEVLAALS
                     STAQADTTVARYAAQDAKRFA"
     gene            complement(2619..3245)
                     /locus_tag="VIJ44_02240"
     CDS             complement(2619..3245)
                     /locus_tag="VIJ44_02240"
                     /inference="COORDINATES: protein motif:HMM:NF013286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HEY5164750.1"
                     /translation="MSDVVEPVLAVTDPAREQILEVRSSEPNPDSLALWVEIDGQSAG
                     AFSYTMELRPVADAGPGDALQRHDDLSVVIAADSIEQLAGATLDFTGAGMVMQNPNKP
                     SAGPARGAPPVADLSGPVPQAVLTILDQEINPAIAAHGGRADLVAVDDGVAYVRMSGG
                     CQGCGLASVTLSQGIEVAILDGVPEIESVIDVTDHASGDNPYFESAKK"
BASE COUNT          467 a         1288 c         1180 g          495 t
ORIGIN      
        1 cgcgcacgga ttcgcggccc gggccgaggc ccacggcgac gagttcacca tcgtgtccga
       61 gtgctgcccg ttcggtgaca ccgcccagcg ctacccgcat gtcgtgtgcg ccctcgatcg
      121 gggcatgatc cgcgggatgc tcgccgggct ctacggcgag acgtcgccgc acttcgagtc
      181 gagccgcccc gacggcagcg accactgcgt cgcccgggtc tgaggcccgg cccgtgacgc
      241 gggcatacct cgaccacgcg tcgtcctcgc cgctgcgccc catggcgctc gaggcgatgc
      301 tcccctacct gcgtgggaac ccggccgacc cgggccgcgt ccacagcgac gggcgggtga
      361 cgcgcgtcgc gatcgaagac gcccgcgccg ccgtcgcggc tctggtcggc gcccgacccc
      421 gcgaagtcgt cttcaccgcc accgggaccg aagcggtgaa caccgcggtg tggggtgcgg
      481 tcacccgcgg cgcggaccgc ctggtgggcc gggggcatgt cgtgaccacc gcagtcgagc
      541 actcggcggt gctcgagtcg tgtcgacgct cagaggtcga cgtgagcatc gtcggcgtcg
      601 acggctcggg ccgcatcgac gaggccgaga tcatggccgc gtgccggccc gagacggtgc
      661 tcgtcagcgt gcagctggcg aaccacgagg tcggcacggt gcagtccgcg gtgccgggca
      721 tcgtcacggc ggtccgcgag cgtggcgtcc tcgtgcacgt cgacgcctgc gccgccgtcg
      781 gtcacgtccc gctcgacttc gccgcgctcg gcgcggacct gtgctcgatc accgggcaca
      841 agctcggcgg acccaagggt tcggcggccc tgctcatccg ccgaggtctg cgccttcccc
      901 cgttcgtggt cggtggcgcc caggagcgcg cccgtcgtgc cgggatcgag gacgtgccgg
      961 ccatcgtcgg cttcggcgcc gccgccgccg agctctacgc cgacgacacg ctcacaaccg
     1021 aggccgggac ggcccgggcc cagaccgacg ggctcctcgc cgccgcgctc gcggtggccg
     1081 gcgtcactca gctcggcgac cccgtcgacc ggctccccca cctggtgtgc ctggccgtcg
     1141 acggggtcga agccgaaccg gtgctcctgg gcctcgacca gcacggtgtg gcggtgcact
     1201 ccggctcgtc gtgctcgagc gagaccctcg agccgtcacc ggtcctcgcc gcgatgggag
     1261 tcgacgccga ccggtcgctg cgccccagcg tcggctggag caccaccgtc gccgacgtcg
     1321 ctgccttcgt cgacgcgttc tcggaggtcg tcgggaagtt acgggccctc ggtgggggtt
     1381 gaaccagtca tgatcccggt cagacacctc aaccacgccg tcctgtacgt gcgagacgcg
     1441 actcagtccg cggcgttcta ccgcgacgtc ttcggcttcc acgcggtcgc cgagctcccc
     1501 ggagccgcgt tcctccgggc cgaggggtcg acgaaccacc acgacctcgg gctgttctcc
     1561 gtgggaccga acgcccccgg ccccgaacca gggcgggtgg ggctgtacca cctggcttgg
     1621 gaggtcgggt cgatcaccga cttggccacc gcccgcgaca cgttgcgccg ggtcggcgcg
     1681 tacgtcggcg agtccgacca cggagcgacg aagtcgatct acggccacga tcccgacggc
     1741 aacgagttcg aggtgatgtg gatggtcccc cgcgacgagt ggggcgacca cgagcacgac
     1801 gcccccgtcg cgcccctcga cctcgacgcc gagatcgccc gacacgggtg acggtcccga
     1861 tcgatagggt gcgcgcatga cgaacgactg gcccgagctc gccgaagggc tcaacgacgc
     1921 cctgaacctc accgcgccac ccatcgcgat cacgttctcc gacgacgcgc cccccggcgt
     1981 ggacccgttc gactcgccca tgcccacccc gaccgacgac ggccgaaccg gtcgcgtacc
     2041 cgccggctgc gtgttctgga tccgcgccgc ggaccggacg ttctccaccg tggccgagga
     2101 tcacgccaac tgctcggtcg gaagcctcac ccacgggttc aagaccctcg acgaggtcgc
     2161 cggcaacgcc gacgtcgccg cgctcctcga cgccggttgg gtcacgatgg atgtcgtccc
     2221 ccagatcccg gtggtacacg accgacccgg gaccgtgacc tacgggccgc tcgctgagac
     2281 cccggtcgac cccgacgtcg tgctggtgcg catcaacggc aagcagctca tggtcctcgc
     2341 cgacgcgctg cccggcctgc gcatcgaggg caagccgcag tgtcacatcg tcgcgatcgc
     2401 caagcaggac ggtgaggtcg cggccagcgt cggctgcgcg ctgagccggg tccgcaccgg
     2461 gatgcccgcc accgagatga cgtgcgcgat tcccgctggg cgcctcccgg aggtgctcgc
     2521 cgcgttgtcg tccaccgcgc aggccgacac gacggttgcc cggtacgcgg cgcaggacgc
     2581 caagcggttc gcctgatcgc gtcgtccggc gcggcgactt acttcttggc tgattcgaag
     2641 tacgggttgt cgcccgaggc gtggtcggtg acgtcgatca ccgattcgat ctcggggacg
     2701 ccgtcgagga tcgcgacctc gatgccctgc gacagcgtga ccgaggcgag cccacacccc
     2761 tgacagccac cggacatgcg cacgtaggcg acgccgtcgt ccactgcgac caggtcggcc
     2821 cggccgccgt gcgcggcgat cgccgggttg atctcctggt cgagaatggt gagcaccgcc
     2881 tgcggcaccg gtcccgacag gtccgccacc ggtggggcac cccgagccgg accggcgctc
     2941 ggcttgttcg ggttctgcat caccatgccg gcgccggtga aatcgagggt cgctccggcc
     3001 agctgctcga tgctgtcggc ggcgatcacc accgagaggt cgtcgtggcg ttgcagcgcg
     3061 tcacccgggc cggcgtcagc gacgggacga agctccatcg tgtacgagaa cgcccccgct
     3121 gactggccgt cgatctcgac ccagagcgcg agcgagtcgg ggttcggctc gctcgagcgg
     3181 acctccaaga tctgctcgcg ggccgggtcc gtcacggcca ggacgggttc gacaacatcg
     3241 ctcatacggc catcatacga ccgtgtctcg gatccttctc ctgctgccga ccgcgacgta
     3301 ccgggcggcc gatttcctcg ccgccgcccg ggcgctcggc gtcgacgtgg tcgtcggcac
     3361 cgaggagccg cagccgcttc ccgccacgat gggcgacggt gccgtggtcg tcccgctcga
     3421 cgacccggac
//
LOCUS       DATCCH010000111         4663 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_47630, whole genome shotgun sequence.
ACCESSION   DATCCH010000111 DATCCH010000000
VERSION     DATCCH010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4663
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47630"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VIJ44_02245"
     CDS             complement(<1..351)
                     /locus_tag="VIJ44_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164751.1"
                     /translation="MESSSQGNQRANRRSHRNGAVCRLLAALGASVILGFGVGAPIAA
                     AQAPAAVPLHDGGGDVGAGAQRGEQLRQVLERLFQQLLAELRGRDHSVQAGSSPPVAT
                     TTPPVATTTPPVTVP"
     gene            478..825
                     /locus_tag="VIJ44_02250"
     CDS             478..825
                     /locus_tag="VIJ44_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY5164752.1"
                     /translation="MSAAPEVPAVDPAEARRRLEAGAMLLDVRETDEWDTGHAPEATW
                     IPMGDVAARQAELAGDQRIVVVCRSGGRSARVTSALLQAGYDATNLAGGMQAWAAAGF
                     SVVTDGGAPGTVA"
     gene            869..2518
                     /locus_tag="VIJ44_02255"
     CDS             869..2518
                     /locus_tag="VIJ44_02255"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HEY5164753.1"
                     /translation="MLSDFDRPEALRRLASDPFDVLVVGGGIVGAGCALDAASRGLRT
                     ALVERQDFASGTSSKSSKLVHGGIRYLQQREFGLVYEGLAERQLALRNAPHLVRVLPF
                     LIPILSKDGLIDPRVARALGAAMWMYDLTGGLRIHKAHQRVAKARALTLMPTLREDNV
                     ASAYLYYDAQADDARLTLCLARTAAAHGAAVANAVRVVGVTKDSDGKVTGARVAADDR
                     EFEIRARTIVNATGVWSDGVRALDEGTQPASIRPAKGIHIAVPWSKVRNEIAAIIPVP
                     KDRRSVFVVPWGDFTYVGTTDTDYDGPIDDPQCTPADVEYLLTALNRAITEPVSTADV
                     TGTWAGLRPLLASASNERTADLSRRHGVRVSTSGVITVTGGKLTTYRRMASDTIDQVG
                     RLLGRRIRARTKHLLLLGAEGFQAPPATPEAASLHEHLAGRYGTEAEHVLALIRENPT
                     WRERLVPGLPYVQAEAIHAARHEMAGTLDDVLSRRTRSRLLARDASRAAADDVARLIA
                     PELGWDAARTRHEVEGFRLACDHERASAQLPESALDASLGA"
     gene            2591..4084
                     /locus_tag="VIJ44_02260"
     CDS             2591..4084
                     /locus_tag="VIJ44_02260"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY5164754.1"
                     /translation="MPTPPIEFGPDPDRTTPVTAHLERAGVAISDASLDRLRSACACV
                     VTDDATLGEASRDWWPLAMIWALDGQVAALAAAVARPSSVEELQAVLRVCDEDRIPVT
                     TAAGRSGVCGASVPVHGGLVLDVCGLTGIVAVDHDSMVLDVRAGTFGDVLEDELRADH
                     GVTVGHWPQSVALSTVGGWLACRGAGQYSTRYGKIEDIVVGLDVVLADGRHLRTGGAP
                     RAAVGPDLTQVFVGSEGTLGVITSARLRLHPTPPSTRTAAWGFPSFTAGLDACRRILQ
                     RDATPAVLRLYDELEGQRNFQTGDLHPLLVLDEGDATMVDAVLAVVGDECRDAVPLDA
                     DLVTRWLEHRNDVSSLEALIQRGFVVDTMEIAARWRDLPPIYAAATAALRDVPGTAVA
                     SAHQSHSYRDGACLYFTFAGQPDAGEREAYYRAAWDAGTRAVLHHGGSLSHHHGIGLN
                     RARFVAEALGGGFEVLVAIKAALDPHGILNPGKLGLTSAFGPSPAWP"
     gene            4110..>4663
                     /locus_tag="VIJ44_02265"
     CDS             4110..>4663
                     /locus_tag="VIJ44_02265"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEY5164755.1"
                     /translation="MTSSERVLVVDVGTSSVRAAVVDAAAHLVAEHREPLLPSSPSPG
                     LVEFDPAVMADVVLTVARAALDDVGPVAAVGVANQRSSTVLWDRATGEPVGVGIGWQD
                     LRTVGRCLELRAEGIRLAPNESATKLEWLLDQADPSRGRDLCFGTVDAWVAWTLSGGA
                     LHITDATNAALTGLRNRGGDDWSE"
BASE COUNT          641 a         1766 c         1600 g          656 t
ORIGIN      
        1 cgggaccgtg accggcggcg tggtcgtcgc caccggcggc gtggtcgtcg ccaccggcgg
       61 cgacgaccct gcctgcacgc tgtggtcgcg tccacgaagc tcagcgagga gctgctggaa
      121 caggcgctcg agcacctggc gcagctgctc accgcgttgg gcgcccgcgc cgacgtcgcc
      181 gcccccatcg tggaggggga ccgcggccgg tgcctgcgcc gccgcgatcg gcgcgccgac
      241 cccgaacccc aagatcacgc tggcgccgag cgcggcgagg agccggcaca ccgcgccgtt
      301 gcggtggctt cgccggttcg ctcgctggtt gccctgggag gagctctcca ccgctgtcct
      361 tacttctgtg cgatgtgtct gccccgccgc cgttcagtgt gtcggcaccg cggcccccaa
      421 accttgagcc atctcccgag gtgcccggcc accggtccgg gtgggaggct gcgggccgtg
      481 agcgcagccc ctgaggtgcc cgccgtggac ccggccgagg cccgccgccg cctcgaggcc
      541 ggagcgatgc tcctcgacgt ccgcgaaacc gacgaatggg acaccgggca tgcaccggag
      601 gcgacgtgga tcccgatggg cgacgtcgcg gcccgccagg ccgagctggc cggcgatcag
      661 cgcatcgtgg tggtgtgccg gtcggggggc cggtcggcgc gggtgacgag cgcgttgctc
      721 caagccggct acgacgcgac gaacctcgcc ggcggcatgc aggcctgggc cgccgccggg
      781 ttcagcgtcg tcaccgatgg cggcgcaccg ggcaccgtgg cgtgaacgcc ccccagccca
      841 acggctactc gccggtaacc tgaggctcgt gctgtccgat ttcgaccggc ccgaggcgct
      901 gcgccgcctc gccagcgacc ccttcgatgt cctcgttgtc ggtggaggca tcgtcggcgc
      961 cgggtgcgcg ctcgacgcgg cctcccgggg cctgcgaacc gccctcgtcg aacgccagga
     1021 cttcgcgtcc gggacctcat cgaagtcctc gaaactggtc cacgggggaa tccggtacct
     1081 ccagcagcgc gagttcgggc tcgtctacga gggtctggcc gaacgccagc tcgcgctgcg
     1141 caacgccccg catctggtgc gggtcctccc gttcctgatc ccgatcctct ccaaggacgg
     1201 cctcatcgac ccgcgcgtcg cccgagcgct cggtgccgcg atgtggatgt acgacctcac
     1261 cggtggcctg cgcatccaca aggcccatca gcgagtcgcc aaggcccggg ccctgacgct
     1321 catgcccacg ctgcgcgagg acaacgtcgc ctccgcgtac ctgtactacg acgcccaagc
     1381 cgacgacgcc cggctcacgt tgtgtctggc ccgcaccgcg gccgcccacg gcgccgcggt
     1441 cgcgaacgcg gtccgagtcg tcggcgtcac gaaagactcc gacggcaagg tcaccggcgc
     1501 acgggtcgcg gccgacgacc gcgagttcga gatccgcgcc cgcacgatcg tgaacgcgac
     1561 cggggtctgg tccgatgggg tgcgcgccct cgacgaaggc acccagccgg cgtcgatccg
     1621 accggcgaag ggaatccata tcgccgtccc gtggtcgaag gtccgcaacg agatcgccgc
     1681 gatcatcccg gtccccaagg accggcgatc ggtgttcgtc gttccgtggg gtgacttcac
     1741 ctatgtcggc acgaccgaca ccgactacga cggtccgatc gacgatcccc aatgcacccc
     1801 agccgacgtc gagtacttgc tgacggcctt gaaccgagcg atcaccgagc cggtatcgac
     1861 ggccgacgtc accgggacgt gggccggctt gcgtccgctg ctcgccagtg ccagcaacga
     1921 gcgcaccgcc gacctgtcgc ggcgccacgg ggtgcgggtc tccacaagcg gggtcatcac
     1981 ggtcaccggc gggaagctca cgacctaccg acgcatggcg tccgacacca tcgaccaggt
     2041 cggtcggctg ctcgggcgac gcatccgggc ccgcaccaag cacctcctgc tcctcggcgc
     2101 cgaggggttc caagcacctc cggcgacccc cgaggccgcc agcctccatg agcacctcgc
     2161 cggtcgctac gggaccgagg ccgagcacgt cctcgcgctc atccgagaga acccgacgtg
     2221 gagagagcgg ctggtccccg ggttgccgta cgtgcaggcc gaagccatcc acgccgcccg
     2281 gcacgagatg gccggcaccc tcgacgacgt gctgtcacgg cgaacccgct cccgactcct
     2341 cgcccgcgac gcctcacgcg ccgccgccga cgacgtcgct cgtctgatcg cccccgagct
     2401 gggatgggac gcggctcgga cccgtcacga ggtcgaaggc ttccgcctcg cctgcgacca
     2461 cgagcgggcc tcggcgcagc tccccgaatc ggccctggac gcgtcgctcg gcgcatgacc
     2521 gggaccgccg cggcgcgaac cgaaccggac cgccgatagg gtcccggccg tgcccgacgc
     2581 tcggcgtggc gtgccgaccc ccccgatcga gttcggtccc gaccccgacc gcaccactcc
     2641 ggtcaccgca cacctagaac gcgccggagt cgcgatctcg gacgcgtcgc tcgaccgact
     2701 ccgatccgcg tgcgcgtgcg tggtcaccga cgacgcgaca ctcggggagg cgagccgcga
     2761 ctggtggccg ctggcgatga tctgggcgtt agacggccag gtcgcggccc tcgccgccgc
     2821 ggtggcgcgt ccgagctcgg tcgaggagct tcaggcggtg ctgcgggtct gtgacgagga
     2881 ccgaatcccg gtgacgaccg ccgcgggacg cagcggggtc tgcggcgcca gtgtcccggt
     2941 tcacggcggt ctcgtcctgg acgtctgcgg gctgaccggc atcgttgcgg tcgaccacga
     3001 ctcgatggtg ctcgacgttc gagcggggac cttcggcgat gtcctcgagg acgagctccg
     3061 cgccgaccac ggcgtcacgg tgggccactg gccccagtcg gtggcgctgt cgacggtcgg
     3121 tggctggctc gcctgccggg gcgccggcca gtactccacc cgttacggca agatcgagga
     3181 catcgtcgtc ggactcgacg tcgtcctcgc cgatggtcga cacctccgca ccggcggcgc
     3241 tccccgcgcc gcggtcggcc cggacttgac ccaggtgttc gtcggcagcg aaggcaccct
     3301 cggggtcatc acctcggcgc ggttgcgcct gcacccgact ccaccctcga cccgaacggc
     3361 agcgtggggc ttcccgtcgt tcaccgccgg gctcgacgca tgccggcgga tcctgcaacg
     3421 cgacgcgacc ccggcggtgt tgcgcctcta cgacgagctc gaaggccaac gcaacttcca
     3481 gaccggagac ctccacccgc tgctggtcct cgacgaaggc gacgccacga tggtcgacgc
     3541 cgtcctggcg gtggtcggcg acgaatgtcg cgacgcggtg ccccttgacg ccgacctcgt
     3601 gacgcgctgg cttgagcacc gaaacgacgt ctcgagcctc gaggccctca tccagcgcgg
     3661 cttcgtcgtc gacacgatgg agatcgcggc gcgatggcgc gacctccccc cgatctatgc
     3721 ggccgcgacg gcagcgctgc gcgacgtccc cgggactgcc gtcgcgtcgg cgcaccagtc
     3781 ccactcgtat cgcgacggcg cctgcctcta cttcaccttt gccggccagc ccgatgccgg
     3841 cgagcgtgag gcgtactacc gcgcggcatg ggacgcgggc acgcgcgcgg tcctccacca
     3901 tggggggtcc ctcagccacc accacgggat cgggttgaac cgggctcggt tcgtggccga
     3961 ggccctcggt ggcggcttcg aggtgctcgt cgccatcaaa gcggcgctcg acccgcacgg
     4021 gatcctgaac cccggcaagc tcgggctcac ctcagcgttc gggccctcac ccgcgtggcc
     4081 ctgagccccc accatcatcg tcgtcgcccg tgacgtcatc ggagcgggtg ctcgtcgtcg
     4141 acgtcgggac atcgtcggtg cgcgccgcgg tcgtcgacgc cgccgcgcac ctcgtcgccg
     4201 agcaccgcga accgctgctc ccgtcgtctc cttcccctgg cctcgtcgag ttcgacccgg
     4261 ccgtcatggc cgacgtcgtg ctcaccgtgg cccgggccgc gctcgacgac gtcggaccgg
     4321 tcgccgcggt cggtgtcgcg aaccagcgca gctcgaccgt gctctgggac cgtgccacgg
     4381 gcgaacccgt cggggtagga atcggctggc aggacctgcg aacggtgggg cggtgcctgg
     4441 agctgcgggc cgaaggcatc cgcctggcac cgaacgagtc ggccacgaag ctcgagtggc
     4501 tcctggacca agcggacccg agccggggtc gtgacctgtg cttcgggacc gtcgacgcct
     4561 gggtcgcctg gaccctctcg ggtggcgccc tccacatcac cgacgccacc aacgccgcgc
     4621 tgaccggctt gcggaaccgg ggcggggacg actggtccga gca
//
LOCUS       DATCCH010000112         3615 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_510264, whole genome shotgun sequence.
ACCESSION   DATCCH010000112 DATCCH010000000
VERSION     DATCCH010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3615
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510264"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..144
                     /locus_tag="VIJ44_02270"
     CDS             <1..144
                     /locus_tag="VIJ44_02270"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017364447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HEY5164756.1"
                     /translation="HVPPSAGTFPTSTPLFVVLVIGVIVIVGALTFLPAVALGPIVEQ
                     LKI"
     gene            159..2219
                     /gene="kdpB"
                     /locus_tag="VIJ44_02275"
     CDS             159..2219
                     /gene="kdpB"
                     /locus_tag="VIJ44_02275"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HEY5164757.1"
                     /translation="MPRDTKLVERDLVRRAVVDSFIKLSPRNLMRNPVMFVVGVGSVL
                     TTILFVKDLGSSSSDQNVFTGLVALFLWFTVLFANFAEAIAEGRGKAQADTLRKTRSE
                     TSARVRQSDGTIEERASTALEVGDECVVSAGEIVPSDGEIIEGIASVDESAITGESAP
                     VIREAGGDRSAVTGGTRVLSDEIVVRITARAGETFLDRMIALVEGSSRQKTPNEIALN
                     ILLAGLTIIFLLATVTLQPFAMFANAEQSIVILVALLVCLIPTTIGALLSAIGIAGMD
                     RLVRRNVLAMSGRAVEAAGDCATLLLDKTGTITFGNRQADTFIPVNGVTEQDLADAAQ
                     LSSLADETPEGRSIVVLAKERYGLRERELVGAELVPFTAQTRMSGVDLDNRSVRKGAA
                     ESVRRWIEEQGGSVPAELGDTVDSIAAQGGTPLVVAEGSTGDGTIAARALGVIYLKDI
                     VKPGIAQRFADLRRMGIRTVMITGDNPRTAAVIAGEAGVDDFLAEATPEDKMELIKRE
                     QRGGHLVAMTGDGTNDAPALAQADVGVAMNTGTQAAKEAGNMVDLDSDPTKLIDIVEI
                     GKQLLITRGSLTTFSIANDVAKYFAIIPAMFAGFASVSVLNDLNVMDLQTPRSAILSA
                     VIFNAVIIVMLIPLALRGVKFVPTGAAQLLRRNLLIYGAGGVVAPFIGIKLIDVLLSA
                     LGVK"
     gene            2220..2945
                     /locus_tag="VIJ44_02280"
     CDS             2220..2945
                     /locus_tag="VIJ44_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011062826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit C"
                     /protein_id="HEY5164758.1"
                     /translation="MRRQLVPALVSMLLLTLLLGVGYPLLVTGIAQVGFQHRADGALV
                     SRNGKPVGSPLIGQAFVDANGNPIPKYFQSRPSAATGVNGTTTAGYDPTLSSGSNLGP
                     SNPKLVAACLPVPETTKSGKPVLDHAGHPVNQHQSDGSLVCDPTTVPQRAKAYRKLNG
                     LRADVAVPVDAVTASASGLDPDISIANARLQAARVGRARHLSARQVLGVVDAHTSGRT
                     LGFLGEKTVNVLEVNLALDRLTS"
     gene            2963..>3615
                     /locus_tag="VIJ44_02285"
     CDS             2963..>3615
                     /locus_tag="VIJ44_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014145350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY5164759.1"
                     /translation="MARGRLRIYLGAAPGVGKTYAMLNEGWRRHARGTDVVVGLYETH
                     GRSNTEAQVRDLEVVPRRTINYRQTTFEEMDVDAVLSRKPEVVLVDELAHTNVPGSRH
                     EKRWQDVEDLLGAGIDVISTLNVQHLESINDVVERITGVKQQETIPDSVVRQAEQVEM
                     VDMTPEAIRRRLAHGNIYPAERVDASLANYFRPGNLAALREIALLWVADRVEENLQQ"
BASE COUNT          590 a         1295 c         1165 g          565 t
ORIGIN      
        1 cacgtgccac cgtcggcggg gacgttcccg acgagcacgc cgctgttcgt ggtgctcgtc
       61 atcggcgtca tcgtcatcgt gggtgccttg acgttcctgc ccgccgtggc gctcgggccc
      121 atcgtcgagc agctcaagat ctgaagggag cagccccgat gcctcgcgac accaagctcg
      181 tcgaacgtga cctggtacga cgcgctgtcg tcgacagctt catcaagctg agcccgcgca
      241 acttgatgcg caacccggtc atgttcgtcg tcggggtcgg gtcggtgctc accaccatct
      301 tgttcgtgaa ggacctcggc tcgtcctcgt cggaccagaa cgtgttcacc ggcctggtgg
      361 ccctcttcct gtggttcacg gtgctgttcg cgaacttcgc cgaggccatc gccgaagggc
      421 gcgggaaggc tcaggcggac acgctgcgca agacccgttc ggagaccagc gcccgcgttc
      481 gtcagtcaga cggaacgatc gaggagcggg ccagcaccgc cctcgaggtc ggcgacgagt
      541 gcgtggtcag tgccggtgag atcgtcccga gcgacggcga gatcatcgag ggcatcgcca
      601 gcgtcgacga atccgccatc accggcgaat cggcgccggt gatccgcgag gccggtggtg
      661 accgctccgc ggtgacgggc ggcacccggg tcctctccga cgagatcgtg gtgcgcatca
      721 cggcccgggc cggcgagacg ttcctggacc gcatgatcgc cctcgtcgaa gggtcgtcgc
      781 gccagaagac gccgaacgag atcgccctga acatcctgct cgcgggactc acgatcatct
      841 tcctgctcgc gaccgtgacc ctgcagccgt tcgcgatgtt cgcgaacgcg gagcagtcca
      901 tcgtcatcct cgtggcgctg ctcgtctgtc tcatcccgac aacgatcggg gcgctgctct
      961 cggcgatcgg gatcgccggc atggatcgtc tggtgcgccg caacgtgctt gccatgagcg
     1021 gacgcgccgt tgaggccgcg ggtgactgcg ccacgctgct cctcgacaag accgggacca
     1081 tcaccttcgg gaaccggcag gccgacacgt tcatcccggt gaacggcgtc acggagcagg
     1141 atctcgccga cgccgcacag ctgtccagcc tcgccgacga gacccccgag gggcgctcca
     1201 tcgtcgtgct cgccaaggag cgctacgggt tgcgggagcg ggagctggtc ggggccgagc
     1261 tcgtcccgtt caccgctcag acccgcatga gcggtgtcga cctcgacaac cgctcggttc
     1321 gcaaaggggc ggcggagtcg gtgcggcgct ggatcgaaga gcaaggcggc tcggtcccgg
     1381 ccgagctcgg cgacaccgtc gattcgatcg cggcccaggg tgggacaccg ctggtcgtcg
     1441 ccgagggatc caccggcgat ggcaccatcg cggcgcgagc gctcggcgtg atctacctca
     1501 aggacatcgt gaagcccggc atcgcgcagc ggttcgccga tcttcgccgc atggggatcc
     1561 gcaccgtcat gatcaccggg gacaaccccc gcaccgcggc ggtcatcgcc ggcgaggcgg
     1621 gcgtcgacga cttcctcgcc gaagccaccc ccgaagacaa gatggagctc atcaagcgcg
     1681 agcagcgcgg cggccatctc gtcgcgatga ccggcgacgg caccaacgac gcgccggctc
     1741 tcgcccaagc cgacgtcggt gtggccatga acaccggaac ccaggcggcg aaggaagccg
     1801 ggaacatggt cgacctcgac tcggacccga ccaagctgat cgatatcgtc gagatcggca
     1861 agcagctgct catcacccgg gggtcgctca cgaccttctc gatcgcgaat gacgtcgcga
     1921 agtacttcgc gatcatcccc gccatgttcg ccgggttcgc cagcgtcagc gtgctcaacg
     1981 acctcaatgt gatggacctc cagacccccc gctccgcgat cttgtccgcg gtcatcttca
     2041 acgccgtgat catcgtcatg ctgatcccgt tggcgcttcg tggcgtcaag ttcgttccca
     2101 ccggcgccgc gcagctcctg cgccgcaacc tgttgatcta cggggcgggt ggtgtcgtcg
     2161 caccgttcat cgggatcaag ctcatcgacg tcctcctctc cgccttgggg gtgaagtaga
     2221 tgcgccgcca gctggtcccc gcgctcgtgt cgatgctgct cctcacactg ctgctcggtg
     2281 tcggctaccc gctcctcgtg accggcatcg cccaggtcgg cttccagcac cgggccgacg
     2341 gagcgctcgt cagccgcaac ggcaagccgg tcggctcccc gctgatcggc caggccttcg
     2401 tcgacgccaa cggcaacccg atcccgaagt acttccagtc ccggccgtcc gccgcgacag
     2461 gggtcaacgg cacgacgacc gcgggatacg accccacgtt gagcagcggg tcgaatctcg
     2521 gtccgagcaa cccgaagctc gttgcggcct gcctcccggt gccggagacc accaagtccg
     2581 ggaagccggt cctcgaccac gccggccatc cggtgaacca acaccaaagc gacgggtccc
     2641 tcgtctgtga tccgaccacc gtgccccagc gcgccaaggc ctaccggaag ctcaacggcc
     2701 ttcgcgccga cgtcgcggtg ccggtcgacg cggtgacggc gtcggcgtcg ggtctcgacc
     2761 ccgacatctc gatcgccaac gctcgcctcc aggcagcacg ggtcggccgg gcccgtcacc
     2821 tctcggcccg ccaggtcctg ggcgtcgtcg acgcccacac ctcggggcgc accctcgggt
     2881 tcctcggcga gaagaccgtc aacgtcctcg aggtcaacct ggccctcgac cggctgacgt
     2941 cgtgatcggg taggaagcgg gcatggcgcg cggtcggctc cggatctacc tgggtgccgc
     3001 gcccggcgtc ggcaagacct acgccatgct caacgagggg tggcggcgcc acgcccgcgg
     3061 caccgacgtc gtcgttgggc tctacgagac ccacggccgg tccaacaccg aagcgcaggt
     3121 tcgtgatctc gaggtcgttc cccggcggac gatcaactac cgccagacga cgttcgagga
     3181 gatggacgtc gacgccgtcc tctcccgaaa gccggaggtg gtgctcgtcg acgagctcgc
     3241 gcacaccaac gtcccggggt cgcgccacga gaagcgctgg caggacgtcg aggatctcct
     3301 cggcgccggc atcgacgtca tctccaccct gaacgtccag cacctcgagt cgatcaacga
     3361 cgtcgtcgag cgcatcaccg gcgtcaagca gcaagagacc atccccgaca gcgtcgtgcg
     3421 ccaagccgag caggtcgaaa tggtcgacat gacgccggag gccatccgtc gtcgcctcgc
     3481 ccacggcaac atctaccctg cggagcgggt cgacgcgtcg ctcgccaact acttccggcc
     3541 cgggaacctc gccgcgctgc gcgagatcgc cctcctctgg gtcgctgacc gggtcgagga
     3601 gaacctccag cagta
//
LOCUS       DATCCH010000113         4848 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_261096, whole genome shotgun sequence.
ACCESSION   DATCCH010000113 DATCCH010000000
VERSION     DATCCH010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4848
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261096"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="VIJ44_02290"
     CDS             complement(<1..671)
                     /locus_tag="VIJ44_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020549732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY5164760.1"
                     /translation="MPGFETLLFEVTDGVATITLNRPDRLNSFDTTLTRELHGLWRDL
                     RFDDAVRAVVLTGAGDRAFCTGIDRGDVIPQPTGPWMMDDPGLSLGPKSADCWKPVIA
                     AVNGMACGGAFYLLGEVETILAAEHATFFDPHVTYGMTAAFEPILMRSRMPFGEIMRL
                     TLLGNYERLSASRAYQVGLVQEVVPGDQLLARAQAVAGEIAASPPVAVQGTVRSIWLA
                     RELRRS"
     gene            747..1547
                     /locus_tag="VIJ44_02295"
     CDS             747..1547
                     /locus_tag="VIJ44_02295"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020555536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5164761.1"
                     /translation="MTSTDDTAVPDYPQSLRLDGRRFLVLGAGQGIGRQSTHALAQLG
                     ARVGCVDVDPDRAAEIATEVGGSPIVGDVTRRLDVERIVEDATRTLGGLDGIVDIVGM
                     AHFAPLLDVTDEEWDWHFDIVLRHAYLALQIGGRALAAAGGGVMAFVASASGITSAPR
                     HAAYGAAKAGLMSLVRTGAVELGPAGVRVNAVAPGVVWTPRISELLGDEGRIRNEANT
                     PLRRVALPADIAGALLFLCSDLAAYVSGQVIVVDGGVGAKFPYPMADA"
     gene            1627..1950
                     /locus_tag="VIJ44_02300"
     CDS             1627..1950
                     /locus_tag="VIJ44_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164762.1"
                     /translation="MFVLVDLGPYPPVISLEEPEDTRRFHVTVRGGADPALVFGALVD
                     AAAGRLEGDDAWITVDAIRRLASGRVQPGWDDEFSTMLDYARTKGWLDEATHALQAHI
                     EWPDA"
     gene            1943..2590
                     /locus_tag="VIJ44_02305"
     CDS             1943..2590
                     /locus_tag="VIJ44_02305"
                     /inference="COORDINATES: protein motif:HMM:NF018777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD domain-containing protein"
                     /protein_id="HEY5164763.1"
                     /translation="MPEPRQQALVAVAWAAGDPPSATIRRWSEHGWRAEPVLPDQGAL
                     DRAGSKGRTPPPFQTLLWRDLTPESPGAAIVPDGLPDVADQWAALRLLEHPAWDDTSR
                     LSLVPPVKQVSFLKAQADLTVTAFRRHFREHVDVARVHHPAICRYTQHDVLDAAGDPG
                     LDVQGVSELWFADEASLVERYFAGPDSVAVVRADNREYIDFSGTLSLLVHPSEPR"
     gene            2587..4701
                     /locus_tag="VIJ44_02310"
     CDS             2587..4701
                     /locus_tag="VIJ44_02310"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEY5164764.1"
                     /translation="MSVEQDALRDTFRGFFARESSPERLRVAEQAEAPGFDADLWSQA
                     RALGVPDLAVDGATLADLAVIAEEYGRALAPIPLLEVLVASRLLDRVPTDRRAAGDED
                     AIVTVALQPVVDGVASLVPAGAVADAVVALHDDALVLARSGRHGVAPPTLGSAPIADW
                     TLDDATVLLEGDAARALFAEAQDDWRALMAAALVGLARRALELGVDYAKARRQFGVPI
                     GSFQSLQHRLADVATLVDAAALLTHHAVTEQRPFPERAAVAYIAAARAARDAAGLSLH
                     VHGGYGFMVEYDIQLYFRRASAWPLALGDPAAELDRLAELLATRGWTIADADAAGFRA
                     EVRQFLEETCPAELVARVHRSGTVHDWEFHRAVAARGLLRVGWGPEWGAEERTDDDVR
                     ALWEELERAGAPIDGWGTSDLVARTLARVGTEEQRRDIVPRVLGGEILICLGYSEPDS
                     GSDVAAASTRAERDGDGWVITGQKMFTTLAHEASYVFLLTRTNREASKHRGLTMFLVP
                     IDQPGIEITPIHTVSGERTNITYYTDVRVPDTCRVGLVDGGWDVMTVALAYERNPTMV
                     GELDRLLRLFLGWARSHPEVLERPVVRDRLARAVIDLEAGRLLSERMSAVGASGALPI
                     VEGSMAKLFSSEALVRASAGLLDTLGAAGVLSVGASGAPAAGWVEVMQRHAQVTTIRA
                     GTSEIQRSIIAERGLGLPRSGR"
BASE COUNT          659 a         1812 c         1675 g          702 t
ORIGIN      
        1 gagcggcgga gctcgcgggc cagccagatg gaccgcaccg tgccctgcac cgccaccggc
       61 ggcgatgccg cgatctcgcc cgcgaccgcc tgggcgcgtg ccagcagctg gtcgccgggg
      121 acgacctcct ggaccaggcc cacctgatac gcccgcgacg ccgacagccg ctcgtagttc
      181 ccgagcaagg tcaagcgcat gatctcgccg aacggcatcc gggagcgcat caggatcggc
      241 tcgaacgccg cggtcatgcc gtacgtgacg tgcggatcga agaacgtggc gtgctccgcg
      301 gcgaggatcg tctccacctc gccgagcagg tagaaggccc cgccgcaggc catgccgttc
      361 accgcggcaa tgaccggctt ccagcagtcg gcgctcttcg gtcccagcga caggccgggg
      421 tcgtccatca tccacgggcc ggtcggctgt gggatgacgt caccgcgatc gatcccggtg
      481 cagaacgcgc ggtccccggc acccgtcagc accacggccc gcaccgcgtc gtcgaaccgc
      541 aagtcacgcc agaggccatg gagctctcga gtcaaggtgg tgtcgaagct gttcagccgg
      601 tcgggtcggt tcaatgtgat cgtggcgaca ccgtcggtga cctcgaacag cagggtctcg
      661 aacccgggca cggcgagagg ctagtgacgg ttgacggtcc gtccccgcgt tcctacggtt
      721 cgctggcgat cgacggagga gcggccatga cgagcaccga cgacaccgcg gttcccgact
      781 acccccagtc gctgcggctg gacggccggc ggttcctggt gctcggcgcc ggccagggca
      841 tcggccggca gagcacccac gccctggccc agctcggggc ccgcgtgggg tgcgtcgacg
      901 tcgaccccga tcgcgcggcc gagatcgcga ccgaggtcgg ggggagcccc atcgtcggtg
      961 acgtcacccg ccggctcgac gtcgagcgga tcgtcgagga cgcgacgcga acgctcggcg
     1021 gcctcgacgg gatcgtcgac atcgtcggca tggcgcactt cgctcccctg ctggatgtca
     1081 ccgacgagga gtgggactgg cacttcgaca tcgtgctgcg ccacgcctac ctcgcgctgc
     1141 agatcggagg ccgggccctc gccgcggccg ggggtggcgt catggccttc gtggcgtcag
     1201 catcgggaat cacctccgcg cctcgccacg ccgcctacgg cgccgccaag gccgggctca
     1261 tgtccctggt gcggacggga gccgtcgagc tcggcccggc cggcgtccgc gtgaacgcgg
     1321 tcgcacccgg tgtcgtctgg accccccgca tctcggagct cctcggcgac gagggtcgga
     1381 tccgaaacga ggccaatacg ccccttcggc gcgtcgccct cccggccgac atcgccggcg
     1441 ccttgctgtt cctgtgctcc gacctggcgg cgtacgtgtc cggccaggtc atcgtcgtcg
     1501 acggcggtgt gggggccaag ttcccctacc ccatggccga cgcctgaccc cgccgaacgg
     1561 ctcggttgcg actgaaacgt ggccgcgaac ctgacgcgcg ggccagcccc gcgataggca
     1621 ggagcgatgt tcgtcctcgt tgacctcggc ccgtaccctc cggtgatctc gctcgaggag
     1681 cccgaggaca cgcgtcggtt ccacgtcacc gtccgaggag gcgccgaccc ggcgctcgtg
     1741 ttcggagctc tcgtcgatgc cgccgccggt cgcctggaag gagacgacgc ctggatcacc
     1801 gtcgatgcga tccgccggct cgcctcggga cgcgttcagc ccggctggga cgacgagttc
     1861 tcgaccatgc tggactacgc ccgcacgaag ggttggctcg acgaggcgac ccacgcgctc
     1921 caagcgcaca tcgagtggcc cgatgcctga accgcgccag caggcactgg tggcggtggc
     1981 ctgggccgcc ggcgacccac cgtccgcgac catccggcga tggtccgagc acggctggcg
     2041 ggcggaaccg gtgcttcccg accagggcgc gttggatcgt gccggctcga agggtcgcac
     2101 gccaccaccg ttccagaccc tgctctggcg tgacctcaca cccgagagtc ccggtgccgc
     2161 catcgtcccc gacggcctcc ccgacgtcgc cgaccagtgg gcggcgctgc ggctgctcga
     2221 gcacccggcg tgggacgaca cgtcccggct gagcctcgtg ccgccggtca agcaggtgtc
     2281 gttcctcaag gcccaagccg acctcacagt cacggcgttc cgacgccact tccgtgagca
     2341 cgtcgacgtc gcccgggtgc accatcccgc gatctgccgg tacacgcagc acgacgtcct
     2401 cgacgccgcc ggcgaccccg gcctcgacgt gcagggcgtg tccgagctct ggttcgcgga
     2461 cgaggcgtca ctcgtcgagc gctacttcgc gggacccgac agcgtcgcag tcgttcgagc
     2521 cgacaatcgt gagtacatcg acttctccgg gacgctctcg ttgctggtcc acccgagcga
     2581 accgcggtga gcgtcgaaca ggacgccctg cgcgacacgt tccggggctt cttcgcccgg
     2641 gagtccagcc cggagcggtt gcgggtcgcg gagcaggccg aagcgcccgg cttcgacgcc
     2701 gacctgtggt cgcaggcccg cgcgctcggt gttcccgacc tggcggtcga cggcgccacc
     2761 ctcgctgacc tggccgtcat cgccgaggag tacggacggg cgctggcacc gatcccgctg
     2821 ctcgaggtgc tcgtcgcgtc gcgtctcctc gaccgggttc cgaccgaccg ccgcgccgcc
     2881 ggggacgagg acgccatcgt cacggtcgcg ctccaacccg tggtcgacgg ggtcgcgtcc
     2941 ctcgtgccgg cgggggcggt cgcggacgcg gtcgtggcgc tccacgacga tgcgctcgtc
     3001 cttgcccgat ccggccgtca cggggtggct ccgccgacgc tcggctccgc gcccatcgcc
     3061 gactggacgc tcgacgacgc caccgtcctc ctcgagggcg acgcggcgcg ggccctgttc
     3121 gcggaagcac aagacgactg gcgcgcgctc atggccgcgg ccctcgtcgg gctcgctcgc
     3181 cgcgcgctcg agctcggtgt cgactacgcc aaggctcgac gccagttcgg ggtcccgatc
     3241 ggctcgttcc aatcgctcca gcaccgcctc gccgacgtcg cgacgctcgt cgacgccgcc
     3301 gcgctcctca cccaccacgc ggtcacggag caacggccct tcccggagcg ggccgcggtg
     3361 gcgtacatcg ctgcggcccg agccgcgcgg gacgcggccg gtctcagcct ccacgtccac
     3421 ggcggctacg ggttcatggt cgagtacgac atccagctct acttccgccg cgccagcgcc
     3481 tggccgctgg ccctcggcga tcccgcggcg gagctcgacc ggctcgccga gctcctcgcg
     3541 acccggggct ggacaatcgc ggacgcggac gcggccggtt tccgggccga ggtccgccag
     3601 ttcctcgagg agacctgccc cgccgagctc gtcgcgcgcg tccaccgctc cggcacggtc
     3661 cacgactggg agttccatcg cgccgtcgcg gcccggggcc tgctccgggt cggctggggc
     3721 cccgagtggg gcgcggagga gcgaaccgac gatgacgtgc gcgcgctctg ggaggagctg
     3781 gagcgcgccg gcgcacccat cgacgggtgg gggacctccg acctcgtcgc ccgcacgttg
     3841 gcgcgagtcg gcaccgagga gcagcgccgc gacatcgtcc cgcgcgtcct cgggggcgag
     3901 atcctcatct gtctcggcta cagcgagccc gactcgggct ccgacgtcgc ggccgcgtcc
     3961 acacgcgccg agcgtgacgg cgacggttgg gtcatcaccg gtcagaagat gttcacgacc
     4021 ctggcccatg aggcctcgta cgtgttcctc ctcacccgta cgaaccggga ggcgtccaag
     4081 catcgcgggc tcacgatgtt cctggtgccg atcgaccagc cggggatcga gatcaccccg
     4141 atccacaccg tgagcggcga gcggaccaac atcacctact acacggacgt gcgcgtcccg
     4201 gatacctgcc gcgtgggctt ggtcgacggc ggctgggacg tgatgaccgt cgccttggcg
     4261 tacgagcgca acccgacgat ggtgggcgag ctcgatcgcc tgctccgcct gttcttgggc
     4321 tgggcccggt ctcaccccga ggtcctcgag cgtccggtcg tccgggaccg tctcgcccgc
     4381 gcggtcatcg acctggaagc cggccggctg ctcagcgagc ggatgagcgc cgtcggcgcc
     4441 tccggtgccc tcccgatcgt cgagggatcg atggcgaagc tgttctcgtc ggaggcgctg
     4501 gtccgcgcgt ccgcggggct gttggacacc ctcggcgcgg ccggggtcct gtcggtcggg
     4561 gcgagcggcg cccccgccgc cggatgggtc gaggtgatgc agcgtcacgc ccaggtcacc
     4621 accatccggg ccgggaccag cgagattcag cgcagcatca tcgccgagcg cggtctcggc
     4681 ctgccccgct ccgggcgctg agccttacgc cagcatcgtc aggggctcgg gcccgtcacc
     4741 cgacacccag accgtgtcgc cgtgcacccg ttcgtcgacc gtcgcctcaa cgaagatcac
     4801 catgtcggcg gagagcacgt cgtcgccgaa cagctcctca tgaccgag
//
LOCUS       DATCCH010000114         2593 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_130568, whole genome shotgun sequence.
ACCESSION   DATCCH010000114 DATCCH010000000
VERSION     DATCCH010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130568"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1116
                     /locus_tag="VIJ44_02315"
     CDS             <1..1116
                     /locus_tag="VIJ44_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HEY5164765.1"
                     /translation="APPGGAFVVASGDTPTFLASWPVTVLDDAELAALLRRLGLAQLG
                     DFAALPPASVLTRFGWSGARAHRLARGLDEHTAPPTPPPPELLETCELDPPATRVDEA
                     AFAAKGLADRLLERLGALGLACNQVVVEAETEHGEHLSRCWRHEGMLTPAALVTRLRW
                     QLDGWLTGQRDGTDDDATGGLVLVRIAPDRVVPAVGRQLGFWGGDAAAGDRADQVLAR
                     LQGMLGPDAVVTASPCGGRLPSERVQWVSWGDARGQDAGPEQPGWPGAVPGPAPARVY
                     EPRLVASLLDAQGAPVTVSGRGEASAAPTRLVAEPIGDAPVIAWVGPWPQDVRWWDRV
                     CRRRRALWQVVARTERADVACLVVMEGGRSGIEAVYD"
     gene            1406..1807
                     /locus_tag="VIJ44_02320"
     CDS             1406..1807
                     /locus_tag="VIJ44_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164766.1"
                     /translation="MFRAAVGVLAAGQRYAPRELVGRAALEVLRGPQRARPDDAGPEL
                     REVYTKVFRTAIQRGELPGTFHPGELGAITNWVLLQGTLFWAVRVSGPLTPEQVLWRR
                     VQLVVHVAGRPGLDPWPGPTGPMPAPCAPPS"
     gene            complement(1767..>2593)
                     /locus_tag="VIJ44_02325"
     CDS             complement(1767..>2593)
                     /locus_tag="VIJ44_02325"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEY5164767.1"
                     /translation="GLAIGFITVALLVVALLTRGAGVVGIAVLFLIFVPIEKLFAFRP
                     QKVFRRGFLTDLTHLLVNNVLITIGLLAAVVVAMVPFFWLRNLDIAASLPPAVAVLIA
                     VAIVFVGQYWAHRLTHQVPLLWRFHSVHHSIENMDWLASGRVHPIDQIFTHGAVVIPL
                     YLLGFNGTVFGGVVVFFTLLALFQHANVRLRFPVVRWVINTPEWHHWHHALDREAINK
                     NFGTPVIDKLFGTAYMPRGQWPSGFGTAEPVPPVGYVRQLAYPFTKVARTGRASGQ"
BASE COUNT          364 a          886 c          969 g          374 t
ORIGIN      
        1 gctccacctg ggggtgcgtt cgtcgtcgcg tcgggagaca cgccgacgtt tctcgcgtcg
       61 tggccggtga cggtcctcga cgacgcggag ctcgcggcct tgctccgacg gctcgggctg
      121 gcacagcttg gtgacttcgc ggcgttaccg cccgcgtcgg tgctcacccg attcggatgg
      181 tcaggggcgc gcgcccatcg gctcgcccga ggcctcgacg agcacaccgc cccgcccacg
      241 ccgccgccgc ccgagctgct cgagacctgc gagctcgacc ctcccgcgac ccgggtcgat
      301 gaagcggcct tcgcggcgaa aggtctcgcg gaccgcctcc tcgaacggct cggagcgctc
      361 gggctggcct gcaaccaggt ggtggtggaa gccgagaccg agcacggcga acacttgtcg
      421 cgctgctggc gccacgaagg catgctcacc cccgcggcgc tggtcacacg gctgcggtgg
      481 cagctcgacg gctggctgac cggccagcgc gacggcaccg acgacgacgc gaccggcggc
      541 ctcgtcctcg tgcggatcgc ccccgaccgg gtcgtcccag cggtcggtcg ccagctcggc
      601 ttctggggcg gtgacgccgc cgcgggggac cgggctgatc aggtgctggc gcgcctacaa
      661 ggcatgctcg gacccgacgc ggtcgtcacc gcgtcgccgt gcggcggtcg tctccccagc
      721 gaacgggtgc agtgggtgtc gtggggtgac gcccgaggcc aggacgcggg cccggagcag
      781 ccaggctggc cgggggcggt tcccggtccc gccccggccc gggtctatga gccgcggctg
      841 gtcgcctcgc tgctcgacgc ccagggggca ccggtcacgg tgtcggggcg cggcgaagcg
      901 tctgcagcgc cgacccgtct ggtggcggaa cccatcggcg acgcgccggt gatcgcctgg
      961 gtcggaccct ggcctcagga tgtgcgctgg tgggatcggg tctgtcgtcg acgacgggcg
     1021 ctgtggcagg tcgtggcccg gaccgagcgg gccgacgtcg cctgcctcgt ggtgatggaa
     1081 ggcggccgga gcgggattga ggcggtctac gactgaacgt cgatcgggta gtgggtcagt
     1141 ttcggccctg cgcagcggtg tgctgctggg ccctaccggc ccgaaggatg ttgatcgcgg
     1201 cgctggtgtc ggcgtgagtc atggccacac gcctggcacc ggctggtgtc gcggggctcc
     1261 actgcgatga cggtccgacc ggcgcttctt ctaccacttc cccgccaagg agcatctcct
     1321 agtcgagctg gtcgcggccg gcggaaccga cgccatcgcc gccgcggtcg acgagcagct
     1381 ggccgcgggg gcatcgctgt cggaggtgtt ccgggccgcg gtcggcgtgc tcgccgcggg
     1441 tcagcgctac gccccgcggg agctcgtggg gcgcgccgcg ctcgaggtgc tgcgcggtcc
     1501 ccagcgcgcg cgtcccgacg acgccgggcc ggagctgcgg gaggtctaca cgaaggtgtt
     1561 ccgcaccgcg atccagcgcg gcgagctgcc gggaaccttt catcccggcg agctcggcgc
     1621 gatcacgaac tgggtgctcc tccagggaac cctgttctgg gccgtcaggg tgagcggccc
     1681 gctcaccccg gagcaggtgc tctggcgacg ggtccagctc gtggtgcacg tcgccggccg
     1741 accgggcctg gacccctggc ccgggcctac tggcccgatg cccgccccgt gcgcgccacc
     1801 ttcgtgaacg ggtacgcgag ctgacggacg tagccgacgg gcggcaccgg ctcggccgtc
     1861 ccgaaccccg acggccactg gcctcgcggc atgtacgcgg tcccgaagag cttgtcgatg
     1921 accggggtgc cgaagttctt gttgatcgct tcccgatcga gggcgtggtg ccagtggtgc
     1981 cactcggggg tgttgatcac ccaccgcacg accgggaatc gcagtcgcac gttggcgtgc
     2041 tggaacaggg ccagcagggt gaagaacacc acgacgccac cgaacacggt gccgttgaag
     2101 ccgagcaggt acagcgggat gacgactgcc ccatgggtga agatctggtc gatcgggtgg
     2161 acccggcctg aggcgagcca gtccatgttc tcgatgctgt ggtgcacgga gtggaaccgc
     2221 cacagcagcg gcacctggtg ggtgagccgg tgcgcccagt actggcccac gaacacgatc
     2281 gcgaccgcga tgaggaccgc gacggccgga ggcaggctcg cggcgatgtc gaggtttcgc
     2341 agccagaaga acgggaccat cgcgacgacg accgcggcga ggagcccgat cgtgatcagc
     2401 acgttgttga ccagcaggtg ggtgaggtca gtcaggaacc cgcggcggaa caccttctgg
     2461 ggtcggaacg cgaacaactt ctcgatcggg acgaagatca ggaacaggac cgcgatcccg
     2521 actacccccg cgcccctcgt cagcaaggcg acgacgagca gggcgacggt gatgaacccg
     2581 atcgcgagcc cgg
//
LOCUS       DATCCH010000115         2607 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_420987, whole genome shotgun sequence.
ACCESSION   DATCCH010000115 DATCCH010000000
VERSION     DATCCH010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420987"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1344)
                     /locus_tag="VIJ44_02330"
     CDS             complement(<1..1344)
                     /locus_tag="VIJ44_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013285209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164768.1"
                     /translation="MKVRARYSALVAGALLAGMVPVLATTAALAGQPVPGHTHLVPQV
                     PRTDQPKISDGEIFDIAAIGNVVYVVGSFTSYTKGTTTIQTNGLAAWNSDTGALLPGF
                     KPVVAGGAVTTVETTPDGTKLFIGGSFKTVNGLAVSKVAELNPTTGAPISTFHFTGTT
                     NNQVTALATTNSTLYVGGKFTKINGSNESGLAAVSTATGAVDTTFTMPLSGGIGVNGE
                     LTVQVLKLTHDESKLIVIHTGRQIAGQDRLGIGIIATATKTLLPWRTELWDNNLARVG
                     GVTRVYAGDVSPDDSYFVVTAGSGGDAPPISDVAVKFPVAGADNVQPIWVSRHFDSVY
                     SVAITEDAIYLGGHFQWQPSPTAPTPWPGLTNVGYGTGQGISAYALGDEVVRVDHLGA
                     LDPNTGDGLEWDPGSNSFQGNKAMLATSRGLFAGGDATIQGGKSGGRIAFFDFNND"
     gene            1548..1952
                     /locus_tag="VIJ44_02335"
     CDS             1548..1952
                     /locus_tag="VIJ44_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017984611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2237 domain-containing protein"
                     /protein_id="HEY5164769.1"
                     /translation="MSEATSDQVNVLGGGLEPCGTDPVTGFYRDGCCTSGREDVGSHT
                     VCSVVSVDFLAHQQGVGNDLSTPRPEYGFPGLSPGDRWCVVAARWLQAYEDGVAPGVV
                     LAATNARALEIIPIAVLRSLAVDVPADPGSLT"
     gene            1976..>2607
                     /locus_tag="VIJ44_02340"
     CDS             1976..>2607
                     /locus_tag="VIJ44_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016189316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY5164770.1"
                     /translation="MTETSSTTAAFRQARDQLLDLRGQHETAVGTFRWPDLGDRFCWA
                     VDWFDAYARDNHQPGLVIVEEDGRSTSLSFAELSHRSDQLAVWLRGQGVAKGDPVILM
                     LGNQVELWESMLAVMKLGAVIMPTTTALGAMDLVDRVTRGGARHVIARADDVAKFADV
                     PGDYGRIRVGPGGASSPGWADYADASRIVDPVVDHPGTAPGDPLLLYFTSG"
BASE COUNT          377 a          851 c          936 g          443 t
ORIGIN      
        1 gtcgttgttg aagtcgaaga acgcgatgcg gccgccgctc ttgccgccct ggatggtggc
       61 gtcgcctccg gcgaacaggc cgcgcgaggt cgcgagcatc gccttgttgc cctggaagga
      121 gttcgatcct gggtcccact ccagcccgtc accggtgttc gggtcgagag cgcccaggtg
      181 gtcgacgcgc acgacctcgt caccgagggc gtatgccgag atgccctggc ccgttccgta
      241 gcccacgttg gtcagaccgg gccacggtgt cggcgccgtc ggggacggtt gccactggaa
      301 gtggccgccg aggtagatcg cgtcctcggt gatggccacg gagtagacgc tgtcgaagtg
      361 ccgcgagacc cagatcggct gcacgttgtc agcgccggcc accgggaact tgacggccac
      421 gtcgctgatc gggggtgcgt cgccaccaga tccggccgtg acgacgaagt acgagtcgtc
      481 cggcgacacg tcaccggcgt agacgcgcgt gaccccgccg acgcgggcga ggttgttgtc
      541 ccacagctcg gtgcgccacg ggagcaaggt cttggtggcg gtcgcgatga tgccgatgcc
      601 gagccgatcc tggccggcga tctggcgacc ggtgtggatc acgatgagct tggactcgtc
      661 gtgggtcagc ttgagcacct ggacggtcag ctcgccgttc acgccgatgc caccgctcag
      721 cggcatggtg aaggtggtgt cgacggcgcc cgtggcggtg ctgaccgctg cgaggccgga
      781 ctcgttcgat ccgttgatct tggtgaactt gccaccgacg tacagcgtgc tgttggtcgt
      841 cgcgagggca gtcacctggt tgttggtggt cccggtgaaa tggaacgtgc tgatgggagc
      901 cccggtggtg gggttgagct cggcaacctt ggagacggcg aggccgttga cggtcttgaa
      961 gctgccgccg atgaacagct tcgtgccgtc cggcgtggtc tcgaccgtcg tcacggcgcc
     1021 gccggcgacg accggcttga agccgggtag cagcgcgccc gtgtcgctgt tccaggcggc
     1081 cagcccgttc gtctggatgg tcgtcgtccc cttggtgtat gacgtgaagg acccgacgac
     1141 gtagacgacg ttgccgatcg ccgcgatgtc gaagatctca ccgtcggaga tcttcggctg
     1201 atcggtgcgc ggcacctgtg gcaccagatg ggtgtgccct gggaccggtt gcccggccag
     1261 cgctgcggtg gtcgcgagga ccggaaccat cccggcgagc agggctccgg cgaccagagc
     1321 ggaatagcgt gcacgaacct tcatgcggtt gcccccaagg catgtcctgc agatgtagcg
     1381 agcgtcacga ggccagcgac ggcactcgtt tgcacaccgt agatgggtgg tgtacgcggc
     1441 ggagtcaggc gatcggccga gaagcactgg tccgttcggt gaccgctctc gcaccttcgg
     1501 acagcccgtg ctcgcagaca tcggctggga ggtgcgaggc tagcgcggtg agcgaggcta
     1561 cgagcgacca ggtcaacgtg ctcggcggtg ggctggagcc gtgcggcaca gatccggtca
     1621 cgggcttcta tcgcgacggc tgctgcacga gcggtcgcga ggatgtcggc agccacacgg
     1681 tctgctcggt cgtgtccgtg gatttcctcg cccatcagca gggggtcggc aacgacctca
     1741 gcacaccccg cccggagtac ggcttccccg ggctctcgcc gggcgaccgc tggtgcgtgg
     1801 tggcggcgcg ctggttgcag gcctacgagg acggcgtggc ccctggcgtg gtcctggccg
     1861 cgacgaacgc tcgggctctc gagatcatcc cgatcgcggt gctgcgctct ctcgccgtcg
     1921 acgtccccgc cgaccctggc tcgctgacct gaccgacccc aggtaggagt ggatcatgac
     1981 cgagacgagt tccacgacgg cggccttccg ccaggcccgc gaccagctgc tcgacctgcg
     2041 cggccagcac gagactgccg tcggaacctt tcgctggccg gacctcggtg accgcttctg
     2101 ctgggcggtc gactggttcg acgcgtacgc gagggacaac caccagcccg gcctcgtcat
     2161 cgtcgaggag gacggccgct cgacgtcact gagcttcgcc gagctgtcgc accgctcgga
     2221 ccagctggcc gtctggctgc gcggtcaggg agtcgccaag ggcgaccccg tcatcttgat
     2281 gctgggcaac caggtcgagc tctgggagtc gatgctcgcg gtcatgaagc tgggtgcggt
     2341 gatcatgccg acgacgaccg cgctcggcgc catggacctg gtcgaccggg tgacgagggg
     2401 tggcgcccgc cacgtgatcg cccgtgccga cgacgtcgcg aagttcgccg acgtccccgg
     2461 cgactacggt cgcatccggg tcggtccagg cggtgcgtcc agcccgggat gggccgacta
     2521 cgcagacgcg tcccgcatcg tcgacccggt cgtcgaccac ccgggcacgg cccccggcga
     2581 cccgttgctg ctctacttca cctcggg
//
LOCUS       DATCCH010000116         2312 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_95950, whole genome shotgun sequence.
ACCESSION   DATCCH010000116 DATCCH010000000
VERSION     DATCCH010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95950"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="VIJ44_02345"
     CDS             <1..393
                     /locus_tag="VIJ44_02345"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEY5164771.1"
                     /translation="VLFLCVHNAGRSQMALGWFQHLASGRALAWSGGSEPTSAVNPAA
                     IAAMAEIGIDISGEYPKPWTDEIVRAADAVITMGCGDACPIFPGKRYEDWELDDPDGL
                     GVEAVRPIRDEIGRRVGDLLASLSLPVG"
     gene            525..1451
                     /locus_tag="VIJ44_02350"
     CDS             525..1451
                     /locus_tag="VIJ44_02350"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY5164772.1"
                     /translation="MDESRATTSRSEAAGRRRRRIALIAVASMVALGAVAAAVGLVPG
                     SGSAPPQAQPEADRSGSGSTTTSAPTTTTTLAPFPTVAPGTVVTNGPRDRRQVALTFD
                     SNLTDGMIQELDQRRVASFANVAVIDELDQLQVPATLFLAGKWIERYPDLTRRLATDP
                     LFELGSHSYEHRAYHVPCYGLGGLPVAQMAADVGHSEQLLRQFTDRPTPYFRFPGGCY
                     DAAALRATAGTGITVIQYDLASGDAFGTSVRTIVNNVVNNAQNGSIVVMHITGGNTAP
                     LTAFALPYVVNGLRQKGFTLVRISELLGPPAP"
     gene            complement(1492..>2312)
                     /locus_tag="VIJ44_02355"
     CDS             complement(1492..>2312)
                     /locus_tag="VIJ44_02355"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEY5164773.1"
                     /translation="AVLAIAVFPGRQVDDLQEVVPRFSRLAFWCVAALVITGGFQAWR
                     QVGSINALRNTDYGQILLVKSLVVLILVVVAALSRQIAGYLFPAPRRVAPAPVPVVAG
                     GSDDLSGDGAVDDDWDEIDERHELKRLRRTVATEIVIAVAVIAVTAVLVNAAPAKIAQ
                     AQPGGAAGVTLKSDQVWVDITAAPGTAPAANDMHVTAINPAGAPVNPADVVATLDYPA
                     RHVAPLTIPLRRLGPGHYLSPGFTIPFKGTWRITAHVRFDQFTEVTIAGPLNIG"
BASE COUNT          351 a          811 c          818 g          332 t
ORIGIN      
        1 gtcctcttcc tctgcgtcca caacgcggga cggagccaga tggccctggg ttggttccag
       61 cacctcgcca gcggccgggc gctggcctgg tccggtggct ccgagccgac cagcgcggtg
      121 aaccccgcgg ccatcgcggc gatggccgag atcggcatcg acatctccgg ggagtacccc
      181 aagccctgga ccgacgagat cgtccgggcc gcggacgccg tcatcaccat gggctgcggg
      241 gacgcctgtc cgatcttccc cgggaaacgg tacgaggact gggagctcga tgaccccgac
      301 gggttgggcg tcgaggccgt ccgtcccatt cgcgacgaga tcggccgacg ggtcggggac
      361 ctgctcgcct cgttgagcct ccccgtcggc tgacccgacc cgcggctcgt tcttcacctc
      421 gaattcgccg ccgatgcctc gacgccgcgg ggcggtcatt gcttgaatgg acgtcgaggg
      481 gaaccagcgg ggctggcgag cgacgcggag tgcgggcagt gcgggtggac gaatcacggg
      541 cgacgacgtc gcgatccgag gccgcgggcc gtcgtcgacg ccgcatcgcc ctcatcgcgg
      601 tcgcctcgat ggtggcgctg ggcgccgtcg cggcggcggt cggactcgtg ccgggatcgg
      661 ggtcggcccc accgcaggcc cagcccgagg cggaccgctc cgggtcgggg tcgacgacga
      721 ccagcgcccc gacgaccacg accacgttgg cgccgttccc cacggtcgcg cccgggacgg
      781 tggtcaccaa cggtccgaga gaccgtcgcc aggtcgcgct gaccttcgat tccaacctga
      841 ccgacgggat gatccaagag ctcgaccagc gtcgcgtggc gtcgttcgcg aacgtggccg
      901 tgatcgacga gctcgaccag ctgcaggttc ccgccaccct gttcctcgcc ggcaaatgga
      961 tcgagcgcta tcccgacctg acccgacgcc tcgccaccga tccgctgttc gagctgggca
     1021 gccactccta tgagcaccgc gcctaccacg tgccctgtta cgggctcgga gggctgcccg
     1081 tcgcccagat ggcggccgat gtcggccact ccgagcagct gctccgccag ttcacggacc
     1141 gtccgacgcc gtacttccgg ttccccggcg ggtgctacga cgccgccgcg ctccgcgcca
     1201 ccgcggggac ggggatcacc gtcatccagt acgacctcgc gagcggcgac gccttcggca
     1261 cgtcagtgcg gaccatcgtc aacaacgtcg tcaacaacgc gcaaaacggc tcgatcgtcg
     1321 tcatgcacat caccggaggc aacaccgcac cgttgaccgc attcgcgctg ccgtacgtcg
     1381 tcaacgggct ccgccagaaa ggtttcacgc tggtccgcat ctcggagctg ttggggccgc
     1441 cggctccctg accgatcccg acgccggcga ccgcgccggc gggcgcggcc atcagccgat
     1501 gttcaacgga ccggcgatcg tgacctcggt gaactgatcg aagcggacat gggcggtgat
     1561 ccgccacgtc cccttgaacg ggatcgtgaa tcccggggag aggtagtggc cggggccgag
     1621 gcgccggagc gggatcgtca acggtgcgac gtggcgcgcc gggtagtcga gggtggcgac
     1681 cacgtcggcc gggttcaccg gcgcccctgc ggggttgatc gcggtgacgt gcatgtcgtt
     1741 ggcggccggc gcggttccgg gcgcggcggt gatgtcgacc cacacctggt cggacttcag
     1801 ggtcacgccg gcggcaccac cgggttgagc ctgggcgatc ttggccgggg ccgcgttgac
     1861 gaggacggcg gtgacggcga tgaccgcgac cgcgatgacg atctcggtgg cgacggtgcg
     1921 ccgcagccgc ttcagctcgt ggcgctcgtc gatctcgtcc cagtcgtcgt cgaccgcacc
     1981 gtcaccggag aggtcgtcgg agcccccggc gacgaccggg accggtgctg gggccacacg
     2041 tcgaggcgcg gggaagaggt aaccggcgat ctggcggctc agcgcggcca ccaccaccaa
     2101 gatgagcacg acgagtgact tcacgagcag gatctggccg tagtcggtgt tgcggagcgc
     2161 gttgatgctc cccacctggc gccaggcctg gaatccaccg gtgatgacga gcgcggcgac
     2221 gcaccagaac gccaaccggg agaagcgagg gaccacctcc tgcaggtcgt cgacctgacg
     2281 gccggggaac accgcgatgg cgagcaccgc ga
//
LOCUS       DATCCH010000117         2024 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_261625, whole genome shotgun sequence.
ACCESSION   DATCCH010000117 DATCCH010000000
VERSION     DATCCH010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261625"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VIJ44_02360"
     CDS             <1..301
                     /locus_tag="VIJ44_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016442944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEY5164774.1"
                     /translation="ERLVAPVRAAGVDTLVLGCTHYPLLARTITDVMGRDVVLVSSAD
                     ETAFAVREMLPNAGGALRHPSRPTFITSGDVEQFRRLGARFLGPEVTDVEAWSWS"
     gene            292..1023
                     /locus_tag="VIJ44_02365"
     CDS             292..1023
                     /locus_tag="VIJ44_02365"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY5164775.1"
                     /translation="MELTVLGCSGSYGGPDGEACSGYLLRDGSTTIWLDCGNGTFGHL
                     QRHVKIEDLTAVVISHEHPDHCVDMYGLHVLMRYGLQRRGLPVYAPAGLGDRLGTLVE
                     GDWGDTFSWNTIDDQQAVTIGSATLRFARTDHPPPTYAVEVTANGSRMIYTSDTGPKW
                     SVEAFAPGADLVLSEATYLHDDKPAPIHLSAKEAGAAARAAGARRLMLTHLWPQLDPK
                     LVVAEGSAAYGQPIMLATPQLVTKI"
     gene            1048..1791
                     /gene="rph"
                     /locus_tag="VIJ44_02370"
     CDS             1048..1791
                     /gene="rph"
                     /locus_tag="VIJ44_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEY5164776.1"
                     /translation="MGSRRDGREPGDLRPVSFTRDYTEFAAGSVLVEFGRTRVLCTAS
                     VEERVPPWLRGKGRGWVTAEYSMLPGSTSERTDREAARGRQSGRTQEIQRLIGRSLRA
                     ITDLKTMGEVQITLDCDVLQADGGTRTASICGAWVALHDACSRLVASGGLTGHPITDV
                     CGAVSVGVVDAIPMLDLDYSEDARAEVDMNVVMTGSGRFIEVQGTAEGMPFSRTELDE
                     LLALAEDGIAQIFELQRALVAEPPTPRRR"
     gene            1806..>2024
                     /locus_tag="VIJ44_02375"
     CDS             1806..>2024
                     /locus_tag="VIJ44_02375"
                     /inference="COORDINATES: protein motif:HMM:NF013854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HEY5164777.1"
                     /translation="MRFVLATDNADKAREIHDVVASTGLPIELLARPGDLPPVDEDRE
                     TLEDNARLKARSVRDATGEAAIADDTGLE"
BASE COUNT          328 a          723 c          672 g          301 t
ORIGIN      
        1 agagcgtctc gtagccccgg tccgagccgc cggcgtggac acgctggtgc ttggttgcac
       61 ccattacccc ctactggccc gtaccatcac cgacgtcatg ggcagagacg tcgtcctcgt
      121 ctcgagcgcc gacgagacgg cgttcgcggt gcgcgagatg cttcccaacg cggggggcgc
      181 cttgcgccac ccgtcgcgcc cgaccttcat caccagcggc gacgtggagc agttccgtcg
      241 gctcggcgcc cggttcctcg gccccgaggt cacagacgtg gaggcctggt catggagctg
      301 acggtgctcg ggtgctcagg gtcctacggc ggaccggacg gggaggcgtg cagcggatac
      361 ctgctccgcg acggctcgac gacgatctgg ctcgactgcg gcaacggaac gttcggccat
      421 cttcaacgcc acgtcaagat cgaagacctc accgcggtcg tgatcagcca cgaacatccc
      481 gaccactgcg tcgacatgta cggccttcac gtgctcatgc gctacggcct gcaacgtcgc
      541 gggctccccg tctacgcgcc ggccggcctc ggcgatcgtc tcggcaccct cgtcgaagga
      601 gactggggcg acacgttctc ctggaacacc atcgacgacc agcaggccgt gacgatcgga
      661 tccgcgacgc tgcggttcgc gcggaccgat cacccgccgc ccacgtacgc cgtggaggtc
      721 acggccaacg gatcccggat gatctacacc tccgacaccg gaccgaagtg gagcgtggag
      781 gcgttcgcgc ccggcgccga cctggtgctg tccgaggcga cctacttgca tgacgacaag
      841 ccggcgccga tccacctgtc ggcgaaggag gccggcgcgg cggcgcgagc cgccggcgcg
      901 cggcggctga tgctgaccca cctctggccg cagctggacc cgaagctggt cgtcgccgag
      961 ggttccgccg cctacgggca accgatcatg ctcgcgacgc cacaacttgt caccaagatc
     1021 tgagaccgag gaccggagcg acactcgatg ggatcccgac gtgacgggcg cgaacccggc
     1081 gaccttcgcc cagtcagctt cacccgcgat tacaccgagt tcgccgccgg gtcggtcctc
     1141 gtcgagttcg gccgcacccg ggtcctgtgc accgcgtccg tcgaggagcg agtgccaccg
     1201 tggttacgcg gcaaggggcg gggctgggtc accgccgagt attcgatgct cccggggtcg
     1261 accagcgaac gcaccgaccg ggaggcggcc cgcggacgcc agtccggacg cacccaggag
     1321 attcaacgcc tcatcggccg gtcgttgcgg gcgatcaccg acctaaagac catgggcgag
     1381 gttcagatca cgctcgactg cgatgtgctc caggctgacg gggggacccg cactgcgtcg
     1441 atctgcggcg cgtgggtcgc gctccatgac gcctgctccc gtctcgtcgc gtccggcggc
     1501 ctgaccggac atccgatcac cgacgtctgt ggtgccgtct ccgtcggggt ggtcgacgcg
     1561 atcccgatgc tggacctcga ctactccgag gacgcacgag ccgaagtgga catgaacgtc
     1621 gtcatgaccg gcagcggccg gttcatcgag gtgcagggca ccgccgaagg gatgccgttc
     1681 tcccgcaccg agctcgacga gctcctcgcg ctcgctgaag acgggatcgc ccagatcttc
     1741 gagttacagc gtgccctcgt ggcggagccg ccgacacctc ggcggcgctg agcagctccc
     1801 ggccgatgcg gttcgtgctc gccaccgaca acgccgacaa ggcccgcgag atccacgatg
     1861 tggtcgcgag caccggtttg ccgatcgagc tgcttgcccg acctggcgac ctcccaccgg
     1921 tcgatgagga ccgcgaaacc ctcgaggaca acgcccgact caaagcgcgc tcggtgcgcg
     1981 atgccaccgg cgaggccgcc atcgctgacg acaccggctt ggag
//
LOCUS       DATCCH010000118         2108 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_213639, whole genome shotgun sequence.
ACCESSION   DATCCH010000118 DATCCH010000000
VERSION     DATCCH010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213639"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="VIJ44_02380"
     CDS             complement(<1..849)
                     /locus_tag="VIJ44_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidyl aminopeptidase"
                     /protein_id="HEY5164778.1"
                     /translation="MAFTFFDDDGFNFEVQNLLGSIRVGCGDAGEILATVATITNGDA
                     ASWVSAWQALGDRVASIADAAASRHYDVSARDAYLRAATYYAAALASVDGADDPDAAL
                     KHVFAQHRRCFDAYADRLQPPAVKVDIPYERSTMPGYLFVPAADGSPRRTVVLNNGSD
                     GAVTSVLGMAKAACDRGYNALIFDGPGQQSMLFDQGIPLRADWEHVITPIVDFLSQRP
                     DVDNDRIALYGISQAGYWVPRALAYEHRIAAAIADPGVVDVSTSWVTHLPKEMLAMLD
                     KRDRTTF"
     gene            1266..>2108
                     /locus_tag="VIJ44_02385"
     CDS             1266..>2108
                     /locus_tag="VIJ44_02385"
                     /inference="COORDINATES: protein motif:HMM:NF015541.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY5164779.1"
                     /translation="MARKRQRSLAEGPSDGSGSAVAATRGNHRGRRWLVVVGAGLLAA
                     AGVLAFVWAVEPANPGSFYDLPLAAHIGVPGSILRQVAVPTNLNGARVSKILYASTDR
                     TNRPIVVSGVIVVPTGPVPPGGRPIIAWAHGTTGVASRCAPSLEPDAGIGRIPLLRDL
                     VAEGVVVVATDYPGLGTPGTHPYLVGESEGRAVLDSVRAAETVDRGSSSAKVVLMGHS
                     QGGHAVLFAAQLAQSYAPDLHLVGVVAMAPPTDLSELLAQDVHENAGVVLTALAITGW
                     THTYA"
BASE COUNT          350 a          686 c          682 g          390 t
ORIGIN      
        1 gaaggtggtt cgatcacgct tgtccagcat ggcgagcatc tcctttggga ggtgcgttac
       61 ccacgacgtc gaaacatcga cgactcccgg gtcggcgatc gcggcggcga tgcggtgctc
      121 gtaggcgagc gcccgcggta cccagtaccc ggcctggctg atgccgtaga gggcgatgcg
      181 gtcgttgtcg acgtcgggac gttgggacag gaagtccacg atcggggtga tgacgtgctc
      241 ccagtccgcc cggagtggga tcccctggtc gaagagcatc gactgctgac ccgggccgtc
      301 gaagatcaac gcgttgtaac cccggtcgca ggcagccttg gccataccga gcactgaagt
      361 gaccgcaccg tcgctaccgt tgttcagcac gacggtcctc cgcggggaac catccgccgc
      421 gggaacgaac aggtagccgg gcatcgtcga gcgctcgtat gggatatcga ccttgactgc
      481 cggcggctgg agccggtcgg catacgcgtc gaagcagcgg cgatgctggg cgaacacgtg
      541 cttcaatgcc gcatctgggt cgtctgcacc gtcgaccgat gcgagcgctg ccgcgtagta
      601 ggtcgcagcc ctcaggtacg catctctggc actcacgtcg taatgccggg atgccgctgc
      661 gtcggcaatg gacgccactc ggtcaccaag cgcttgccac gcggacaccc agctcgcggc
      721 atcgccgttg gtgatcgtcg ccacggtggc caggatctcg ccggcatcac cacaaccgac
      781 gcgaatcgac ccgagcaggt tctggacctc gaagttgaat ccatcgtcgt caaagaatgt
      841 gaatgccact gagctcactc cttctcgctt gcagcaactg catcgttgct gaggtgatgt
      901 gctgctcgcg ttcgccatga gtggtcggtc gaggtgaact cgcttcatct tggcacgacg
      961 cggaaccagg gtcagagtcc ccgactctcc aagatgcgag tctcgcgagc tcatcgacct
     1021 gactcgccat gggcccgacg ctgttggggt cacggtggcc gggatcgtcg gcgttgaggc
     1081 cccaggcccc cagtgaaccc cggagccacc catctgagac gttgatgctg cgcaaggaac
     1141 caggagccct tgggccagtg acgcctgagg agtgtcgtcg atgtggtccg cgcctgtagc
     1201 gtccgaggaa tgaggattct ggtttctgaa gcggcgacag ctctgctgcg tcgagggagg
     1261 agtccatggc ccgcaagcgg caacggtcac tagcggaagg gccgtccgat gggagcggct
     1321 cggctgtcgc cgcgactcga ggtaatcatc gcggccgccg ctggctggtc gtggttggag
     1381 ctggcctcct cgccgccgcc ggtgtcctcg ctttcgtgtg ggcagttgaa ccggcaaatc
     1441 ccggttcgtt ctacgacctg cccttggcgg cccacatcgg ggtaccgggt tcgatcctca
     1501 ggcaggtggc ggttccgacc aacctcaacg gtgcccgcgt ctcaaagatc ctctatgcat
     1561 ccaccgatcg aaccaatcgc ccgattgtcg tatccggtgt catagtcgtg ccaaccggtc
     1621 cggtacctcc cggcggtcga ccgattattg cctgggcaca cggcaccacc ggcgtagcct
     1681 cccgatgcgc cccgtcgctc gagcccgacg cgggcatcgg tcgcataccc ctactccgtg
     1741 acctcgtggc cgagggagtg gtggtcgtgg ctactgacta ccctggtctc ggtacgcccg
     1801 gcacgcatcc ctaccttgtc ggcgagagcg aaggccgggc ggtgctggac agcgtccgag
     1861 cggctgagac ggtggatcga gggtcatcgt cggccaaggt cgtgctcatg ggtcactccc
     1921 aaggcggcca tgccgtgctc ttcgccgctc aactggccca gtcctacgct cccgacctcc
     1981 acttggtcgg agtggtcgcc atggcgccgc cgaccgatct gagcgaactc ctggcccagg
     2041 acgttcacga aaatgctggc gtcgtcttga ctgcactcgc catcaccggc tggacccaca
     2101 cgtatgcc
//
LOCUS       DATCCH010000119         3278 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_178893, whole genome shotgun sequence.
ACCESSION   DATCCH010000119 DATCCH010000000
VERSION     DATCCH010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178893"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2214
                     /locus_tag="VIJ44_02390"
     CDS             <1..2214
                     /locus_tag="VIJ44_02390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY5164780.1"
                     /translation="DDFEMTSRALDEVRRGTRGPNESLVVRIRAADGSYHAFETVAQD
                     LTADPAVGGIVLNSRDVTERVDAEEQLRVADGRLRTLLEHSSDLIVLLDPTGAIEYIS
                     PAHEAMFGRRTDEVEGTDGLFAVHPDDLPRIAATMASLSDDPGRRVNVTLRVRHVDGS
                     YRHVEAIGQNRTGDAAVHGIVWNVRDITDRIRTEAELRATEQRLAAVVEHSSDLIVVL
                     DEEGRVRYLSPASERLFGRSAEGGVGVLGTELVHPDDLERVGAKFAQLAGSPGASDMV
                     TCRVLHTDGSYRWVEAVGRNRLDDSALPGIVLNIRDITDRIESETTLRAAQARFAALV
                     SHASDLITVNDLHGVVTYASPSALTILGWEPDELVGTQARDLLHPDDVAEVEHAAADQ
                     FAGKRVEPIEYRARHRDGSWRVLDAIVTNLVDEPAVQGVVTNARDVTDRRAAERRAAD
                     LVEILEAANELVVVSDPAGRIVYANRSARSLLGAHEQRHVGELSSERSRERLRTEIMP
                     VVRRRGAWSGELELVDSEGRRIPVTATVQAQRDESGDVVRVATVAHDISELKAAQRRL
                     EFEATHDALTGLPNRALFREIGDRAVARASRISESLAVLFLDLDGFKLVNDSYGHDTG
                     DMLLGQVACRLREAVRAGDFVARLGGDEFVILCEHPRNEQQMLELSTRIIETVSQPFV
                     IDEHSVRVGVSVGIAFSKGSNAGIGELIRDADVALYRAKNEGRGRAQLFDATLLG"
     gene            complement(2221..2778)
                     /locus_tag="VIJ44_02395"
     CDS             complement(2221..2778)
                     /locus_tag="VIJ44_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164781.1"
                     /translation="MAVIDVAGFVADLKDHAVEHGFHVHDERHFVESYSLRQTWEVDV
                     HPEEGCEGPVDLYLSLEIDPRVLLGFEDAVMTRPEGDDPSDDYEFPLNFTWGLPPLPH
                     NPDLLVLATDLAGIGGPELPLEVSAIDSIPSATDSPERALRVVAHQRVSLLRIRDGEA
                     VSCELLDRCLEVSRFLLARAGGWLG"
     gene            complement(2780..>3278)
                     /locus_tag="VIJ44_02400"
     CDS             complement(2780..>3278)
                     /locus_tag="VIJ44_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HEY5164782.1"
                     /translation="RSVRDATGEAAIADDTGLEVDALGGAPGVRSARFAGPHATDHDN
                     LTLLLERLRGVPFEERRARFVTVAVASFPDGRELAAIGTVEGTIASQPRGDGGFGYDP
                     VFLADPGDGRTFAEMSPEEKHTCSHRGRAFRTLADGLAILDRAGHLDPPDPSGGGPAN
                     DPEEV"
BASE COUNT          514 a         1126 c         1162 g          476 t
ORIGIN      
        1 gacgacttcg agatgacaag tcgggcgctc gacgaggtcc ggcgcggcac gcgaggaccg
       61 aacgagtcgc tcgtggtgcg gatccgcgcc gcggacggct cgtaccacgc gttcgagacc
      121 gtcgcccaag acctcaccgc ggaccccgcg gtgggcggca tcgtcctgaa ctcacgcgac
      181 gtcaccgaac gggtcgacgc cgaggagcag ctccgcgtcg ccgacggccg cctccggacc
      241 ctgttggagc attcgtcgga cctcatcgtg ctcctggatc cgactggcgc catcgagtac
      301 atcagccccg cccacgaggc gatgttcggt cggcgcaccg acgaggtcga aggcacggac
      361 gggttgttcg cggttcaccc cgacgacctg cctcggatcg cggcgacgat ggcttcgctg
      421 tccgacgacc ccggccggcg cgtgaacgtc acgctccggg tccgacatgt cgacggctcg
      481 taccgacacg tggaggccat cggccagaac cggaccggag acgccgcggt gcacggcatc
      541 gtctggaacg tccgcgacat cacggaccgc atccgcaccg aagccgagct acgcgccacc
      601 gagcagcgcc tcgccgccgt cgtcgagcat tcgtcggacc tcatcgtcgt cctcgacgag
      661 gagggtcggg tccgctactt gagcccggcc agcgagcgcc tgttcgggcg cagcgccgaa
      721 gggggggtcg gggtgctcgg gaccgagctc gtgcatcccg acgacctcga gcgggtcggc
      781 gccaagttcg cgcaactcgc gggctcgccg ggcgccagtg acatggtgac ctgccgggtg
      841 ttgcacaccg acgggagcta ccgctgggtg gaagcggtgg gccgcaaccg gctcgacgat
      901 tcagcgctac ccgggatcgt gctcaacatc cgcgacatca ccgaccggat cgagtcggaa
      961 acgaccctgc gagccgccca ggcccggttc gccgcgctgg tgtcccacgc ctcggatctc
     1021 atcaccgtga acgaccttca cggagtcgtc acctacgcga gcccctcggc cctgacgatc
     1081 ctcggctggg aaccggacga gctggtcggc acccaggccc gcgacctcct ccaccccgac
     1141 gacgtcgccg aggtggagca cgccgccgcc gaccagttcg ccgggaagcg agtcgagccc
     1201 atcgagtacc gcgcccggca ccgcgacggc tcgtggcggg tgctcgatgc catcgtgacg
     1261 aacctggtgg acgagcccgc ggtccagggc gtcgtcacga acgcgcgcga cgtcaccgac
     1321 cgccgagcgg cggagcggcg agcggccgac ctcgtcgaga tcctcgaggc ggccaacgag
     1381 ctcgtcgtgg tctcggatcc cgccggtcgg atcgtctacg cgaatcggtc cgcccggagt
     1441 ctcctcggcg cgcacgagca gcgccatgtc ggtgagttgt cgtcggaacg gtcgcgggag
     1501 cggctgcgga ccgagatcat gccggtagtg cggcgacgcg gggcgtggtc cggcgagctg
     1561 gagctcgtcg actccgaggg tcgacggatc ccggtcacgg ccaccgtcca agcccaacga
     1621 gacgagagtg gcgacgtggt gcgcgtggcg acggtcgcgc acgacatctc cgagctcaaa
     1681 gccgcgcagc gccgcctcga gttcgaagcc acccatgacg ccttgaccgg gttgccaaac
     1741 cgggcactgt ttcgtgagat cggggaccgg gcggtcgctc gcgccagccg catctcggag
     1801 tcgctcgccg tgttgttcct cgacctcgac ggcttcaagc tcgtgaacga cagctacggc
     1861 cacgacaccg gagacatgct cctcggtcag gtcgcctgtc gcctacgcga ggcggtgcgg
     1921 gccggtgact tcgtcgctcg cctcgggggc gacgagttcg tgatcctctg cgagcatcct
     1981 cgaaacgagc aacagatgct ggagctgtca acccggatca tcgagacggt ctctcagccg
     2041 ttcgtcatcg acgagcattc ggtgcgcgtc ggtgtcagtg tgggcatcgc gttctcgaag
     2101 ggttccaacg cggggatcgg tgagctgatc cgagacgccg atgtcgcgct gtaccgagcc
     2161 aagaacgagg ggcggggccg cgcccagttg ttcgacgcga ccctgctcgg gtaaagccgg
     2221 tcagcccagc cagccgccgg ctcgggcaag caggaatcgg ctcacttcga ggcaacggtc
     2281 cagcagctcg cacgacaccg cctcaccgtc gcggatgcgc agcagcgata cccgctggtg
     2341 cgccacgacc cgcaacgccc gttcgggaga gtcggtcgct gacgggatcg aatcgatcgc
     2401 cgacacctca agggggagtt caggaccgcc gatcccggcc aggtcggtcg ccaggacgag
     2461 caagtcgggg ttgtggggca atgggggcag cccccaggtg aagttgaggg ggaactcgta
     2521 gtcgtcggat gggtcgtcac cctcgggacg ggtcatgacc gcgtcctcga acccgaggag
     2581 cacccgaggg tcgatctcga gcgacaggta gaggtcgacg gggccctcgc agccctcttc
     2641 ggggtgcacg tcgacctccc aggtctggcg cagcgagtag ctctcgacga agtgccgttc
     2701 gtcgtggacg tggaagccgt gctcgaccgc gtggtccttg aggtcggcga cgaagccggc
     2761 aacgtcgatg acggccacgt cacacctcct cggggtcgtt ggccggaccg ccgccgctcg
     2821 ggtctggcgg gtcgagatga ccggcgcggt ccaagatcgc cagtccgtcg gcgagcgtac
     2881 ggaaggctcg gccccgatgc gagcaggtgt gcttctcctc ggggctcatc tcggcgaagg
     2941 tgcgcccgtc gccggggtca gccaggaaga cggggtcgta gccaaacccg ccgtcccctc
     3001 ggggctggct ggcgatggtg ccctccacgg ttccgatcgc cgcgagctcg cgtccgtcgg
     3061 ggaagctcgc caccgcgacg gtgacgaagc gggcgcgtcg ttcctcgaag gggacgccgc
     3121 ggagccgttc gaggagcagg gtcaggttgt cgtggtcggt ggcgtgcgga ccggcgaagc
     3181 gggcggagcg cactcccggg gcacccccca gcgcgtcgac ctccaagccg gtgtcgtccg
     3241 cgatggcggc ctcgccggtg gcgtcgcgca ccgagcgc
//
LOCUS       DATCCH010000120         2938 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_130770, whole genome shotgun sequence.
ACCESSION   DATCCH010000120 DATCCH010000000
VERSION     DATCCH010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130770"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="VIJ44_02405"
     CDS             <1..618
                     /locus_tag="VIJ44_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D 1"
                     /protein_id="HEY5164783.1"
                     /translation="DLPWGWVAECRSTMKVVLDACDDFEDLVLGNEIFEARTRGIGII
                     PGRIGASYGVSGSNLRASGVDWDLRRDGPPYLVYPDLDFKVWTHPDGDSYSRYWVRLQ
                     ETRESARMVLQCLDAIPSGPIMAKVPRIIKVPEGEIWVETENPLGQMGYYVISKGATG
                     PFRVKIRSASFSNVSIVPWLLRGVYVPDVITILASLYFILGDIDR"
     gene            618..1655
                     /gene="nuoH"
                     /locus_tag="VIJ44_02410"
     CDS             618..1655
                     /gene="nuoH"
                     /locus_tag="VIJ44_02410"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005313708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEY5164784.1"
                     /translation="MATIPTLALSIGWGTTLAIKTIVILMVIPLGALVLGYVFLMKMM
                     SHMQSRLGPMDPGGFHGWYQLVGDGLKFLQKEDIMPAESDRRVFAAAPAVVVMSTFLV
                     FLVIPMGPDLVVKNLDVGVFYALAVSSLSVIGVLMAGWASANKFALLGALRAAAQLIA
                     YELPLLLAVVGVVIQAGTMSLQGIVLSQQRGSVFGWSGLGEPFVLTQFIGFALFLVAS
                     QAELTQTPFDMPVAESELVAGYMVEYTGFRFLFFFIGEFGTAFAFSAIAATLFLGGWA
                     IPGVNGDLANILGPMVLFAKLMMVAFLMFWVRFTYPRLREDQLQALAWKFLIPLGLAN
                     ILLTGIFKVAL"
     gene            1655..2137
                     /locus_tag="VIJ44_02415"
     CDS             1655..2137
                     /locus_tag="VIJ44_02415"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HEY5164785.1"
                     /translation="MKPKIPGLIGGLGVTLKTMLRPAVTVQYPHEKEAPAPRARGVIA
                     LKEENCTVCMLCARQCPDWCIYIEGHKEQRPPRREGGRPRSVSVLDRFDIDYALCMYC
                     GICVEVCPFDALFWSPEYEYSEFQISHLLHDKVKLGEWMESVPEPEPLEVGAESKGKK
                     "
     gene            2142..2648
                     /locus_tag="VIJ44_02420"
     CDS             2142..2648
                     /locus_tag="VIJ44_02420"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018550687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEY5164786.1"
                     /translation="MWEQQVAFWLFAVAMGAAAIGVVRSRNVVHAALFLVVVLAGAAA
                     QYILLAAEFVAWVQVLIYIGAVVILFLFGVMLTRAPMRGEGESLDNDQRWGAIVAALF
                     VLGVLVALFVDAFGGKKIHFSHALVALGRTSTVGQDIFRVFVVPFEIVSMLLLAALVG
                     AVVLARKD"
     gene            2650..>2938
                     /gene="nuoK"
                     /locus_tag="VIJ44_02425"
     CDS             2650..>2938
                     /gene="nuoK"
                     /locus_tag="VIJ44_02425"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEY5164787.1"
                     /translation="MQLNEFLILSAFLFCTGIYGVLARRNGVLVLMSVELMLNAVNIN
                     LIAFSAFTRNISGQVFALFVITIAAAEVGVGLAIVLLIYRNLRSPNLDEIDQ"
BASE COUNT          444 a          979 c          928 g          587 t
ORIGIN      
        1 gacctcccgt ggggatgggt cgccgagtgc cgctccacga tgaaggtcgt gctcgacgcc
       61 tgcgacgact tcgaggacct cgtgctcggc aacgagatct tcgaagcccg cacccgcggc
      121 atcgggatca tcccgggccg catcggcgcg tcctacggcg tgtccgggtc caacctgcgc
      181 gcgtccggcg tcgactggga cctgcggcgt gacggccccc cgtacctcgt gtacccggat
      241 ctcgacttta aggtttggac ccaccccgac ggtgactcgt actcgcgcta ctgggtccgg
      301 ctccaagaga cccgcgaatc cgcccggatg gtgctgcagt gcctcgacgc catcccgagc
      361 ggcccgatca tggcgaaggt gccgcggatc atcaaagtgc cggaaggcga gatctgggtc
      421 gagaccgaga acccgctcgg gcagatgggg tactacgtca tctcgaaggg cgcgaccggt
      481 ccgttccggg tgaagatccg ctccgcgtcg ttcagcaacg tgtcgatcgt cccttggctg
      541 ctgcgcggcg tctacgtgcc cgacgtcatc acgatcttgg cgtcgctcta cttcatcctc
      601 ggtgacatcg accggtgatg gcgaccatcc ccacgctcgc gctgagtatc ggttggggca
      661 cgacgctcgc gatcaagacg atcgtgatcc tcatggtcat cccgttgggg gcgctggtcc
      721 tcggctacgt cttcttgatg aagatgatga gccacatgca gagccgtctc ggcccgatgg
      781 accccggcgg cttccacggc tggtaccagc tcgtcggcga cggcctgaag ttcctccaga
      841 aggaggacat catgccggcc gagtccgacc ggcgagtgtt cgccgcggcg ccggccgtgg
      901 tcgtcatgtc gaccttcttg gtcttcctgg tgatcccgat gggacccgac ctggtcgtca
      961 agaacctcga cgtgggggtc ttctacgccc tcgccgtgtc gagcctctcg gtcatcgggg
     1021 tactcatggc cgggtgggcc agcgcgaaca agttcgcgct gctcggcgcg cttcgagccg
     1081 cggcgcagct catcgcctac gagctgccgc tgctcctcgc ggtcgtcggt gtggtcatcc
     1141 aggccggcac catgagcctg caaggcatcg tgctctccca gcagcgcggc tcggtcttcg
     1201 gttggagtgg tctcggcgag ccgttcgtct tgacgcagtt catcggattc gcgctgttcc
     1261 tcgtcgcctc ccaagccgag ctcacccaga caccatttga catgccggtc gccgagagcg
     1321 agctcgtcgc gggctacatg gtcgagtaca ccgggttccg gttcctgttc ttcttcatcg
     1381 gcgagttcgg caccgcgttc gcgttctcgg cgatcgcggc caccttgttc ctcggcggtt
     1441 gggccatccc cggagtcaac ggcgacctcg ccaacatcct cgggcccatg gtcctcttcg
     1501 ccaagctgat gatggtcgcg ttcctcatgt tctgggtgcg attcacgtac ccgcggctgc
     1561 gcgaagacca gctgcaggcc ctggcctgga agttcctcat tccgctgggg ctcgccaaca
     1621 tcttgctcac cggcatcttc aaggtggcgc tctgatgaaa cccaagattc ccggcctgat
     1681 cggcgggctc ggagtgacgc tcaagaccat gttgcgcccc gcggtgaccg tgcagtaccc
     1741 gcacgagaaa gaagcccccg cgcctcgggc tcggggagtc atcgccctca aggaagagaa
     1801 ctgcaccgtc tgcatgctct gcgcacgcca gtgcccggac tggtgcatct acatcgaagg
     1861 gcacaaggag cagcgacccc cacgccgaga aggcggccgc ccccggtcgg tgagtgtgct
     1921 tgaccgcttc gacatcgact acgcgttgtg catgtactgc gggatctgcg tcgaggtgtg
     1981 ccccttcgac gcgctgttct ggagccccga gtacgagtac tcggagttcc agatctcgca
     2041 cctgctccac gacaaggtca agctcgggga gtggatggag tccgtcccgg aacccgagcc
     2101 actcgaagtt ggggccgagt cgaagggtaa gaagtagcgc cgtgtgggag caacaggtcg
     2161 ccttctggct tttcgccgtc gcgatgggtg ccgcggcgat cggagtggtc cggtcccgca
     2221 acgtcgtcca cgcggccttg ttcctcgtcg tcgtcctcgc tggtgcagcg gcccagtaca
     2281 tcttgctcgc ggccgagttc gtcgcctggg tgcaagtcct catctacatc ggcgcggtgg
     2341 tcatcctctt cctcttcggg gtgatgctca cccgcgcccc catgcgaggc gagggagaga
     2401 gcctggacaa cgaccagcgg tggggcgcga tcgtggcggc cctcttcgtc ctcggggtgc
     2461 tcgtcgcgct gttcgtcgac gcattcgggg gcaagaagat ccacttcagt cacgcgctcg
     2521 tcgcgctggg gcgcaccagc accgtgggac aggacatctt ccgcgtcttc gtcgtgccgt
     2581 tcgagatcgt ctcgatgctg ctcctcgccg cattggtcgg cgccgtcgtg ctcgcccgaa
     2641 aggactgagg tgcagctcaa cgagttcctg atcctcagtg ccttcttgtt ctgcaccggc
     2701 atctacggag tgctcgctcg ccgcaacggc gtgttggtgc tcatgagcgt cgagctgatg
     2761 ctcaacgcgg tcaacatcaa cctcatcgcg ttctcggcgt tcaccaggaa catcagcggc
     2821 caggtgttcg ccttgttcgt catcaccatc gctgcggcgg aggtcggtgt cggactggcc
     2881 atcgtgctgc tcatctatcg caatctgcgc agcccgaacc tcgacgaaat cgaccagc
//
LOCUS       DATCCH010000121         2204 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_380149, whole genome shotgun sequence.
ACCESSION   DATCCH010000121 DATCCH010000000
VERSION     DATCCH010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380149"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1319)
                     /locus_tag="VIJ44_02430"
     CDS             complement(<1..1319)
                     /locus_tag="VIJ44_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014143088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5164788.1"
                     /translation="MLDVVIKDATVVDGTGTPGRPGDVAVQDGRIVAVGTVDDAAAET
                     IDADGLVVTPGFVDPHTHYDAQLFWDPSASSSSLHGVTSIVAGNCGFTLAPIAERDAD
                     YTRRMMAKVEGMPLAALEQGVPWNWSTFAEYLDRLDGQVGVNVAFLVGHCALRRHVMG
                     ADAVGNEATPDQVAAMVGLLHESLEAGGLGFSTTLSFTHSDGDGQPVASRWASRDEVL
                     ALCGAVRDHEGTTLEYVTDGCLRGFRPDEVELMAAMTLAGRRTLNWNVLTVDAKEPDR
                     YRDQLAANEHAAAAGGRSIALTMPVLVEMNMSFLNYCALFMLPDWGAVMNLPVAERMA
                     KLRDPQVRRHLNDRAHSPDAGVFTRLTDWGRYQIGDTFSAENEGLKGRAVADLARERG
                     TAPFETLLDVVLADELRTVLWPIPTDDDGDSWDLRRDAWARDDVLIGG"
     gene            1541..1963
                     /locus_tag="VIJ44_02435"
     CDS             1541..1963
                     /locus_tag="VIJ44_02435"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY5164789.1"
                     /translation="MDHGKTEEWLQKVPLFRGLSKKQLRDISGLATRLDEPAGTVLTK
                     EGKIGHEFIIVIEGEIEVRKGDQVIATRGPGDYVGEIALMDHRPRTATVVAKTPVVIE
                     VIGQREFRTLLAEAPGLSSDIMSTMAQRLAELDQSGEG"
     gene            complement(1970..>2204)
                     /locus_tag="VIJ44_02440"
     CDS             complement(1970..>2204)
                     /locus_tag="VIJ44_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017945601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
                     /protein_id="HEY5164790.1"
                     /translation="VAVVGVPDDRLGEVGMAFVVARPGATVDPDEFIAWSRDHVANYK
                     VPRYVEVMDALPLNASGKVLKYELRDRAQVRNR"
BASE COUNT          379 a          746 c          741 g          338 t
ORIGIN      
        1 ccgccgatca ggacgtcgtc gcgggcccag gcgtcacgtc gcaggtccca ggagtcgcca
       61 tcgtcgtcgg tcgggatcgg ccacagcacc gttcgcagct cgtcggcgag gacgacatcc
      121 agcagggtct cgaacggtgc cgtcccgcgc tcgcgggcca ggtccgccac cgctcggcct
      181 ttgagtccct cgttctcggc cgagaaagtg tcgccaatct ggtaccggcc ccagtcggtg
      241 agccgggtga agacaccggc atcgggggaa tgcgcgcgat cgttgaggtg acgccggacc
      301 tgtggatcac gcagcttggc catccgttcg gctaccggca ggttcatcac tgcgccccag
      361 tccggcagca tgaacagggc gcagtagttc aggaaactca tgttcatctc gacgaggacc
      421 ggcatcgtga gcgcgatcga tcggcctccc gcggccgcag catgctcgtt cgcggcaagc
      481 tgatcgcggt accggtcagg ttccttcgcg tcgacggtca acacgttcca gttcaacgtc
      541 cgccgccccg cgagggtcat ggctgccatc agctcgacct cgtcgggtcg aaagccacgg
      601 aggcagccat cggtgacgta ctcgagcgtc gttccctcgt ggtcgcggac cgcaccgcac
      661 aacgcaagga cctcgtcgcg gctcgcccag cgggacgcga ccggttggcc gtcgccgtca
      721 gagtgggtga acgagagcgt cgtggagaag ccgagcccac cagcctcaag ggattcgtgc
      781 aagaggccga ccatcgccgc gacctggtcg ggcgtcgctt cattcccgac cgcatcggca
      841 cccatcacat gacgccggag ggcgcagtga ccgaccagga acgcgacgtt gacgccaacc
      901 tggccgtcga gtcggtcgag atactcggca aaggtcgacc agttccaggg gacgccttgc
      961 tcgagcgcgg cgaggggcat cccctcgacc ttcgccatca tgcggcgcgt gtaatccgcg
     1021 tcgcgttcgg cgatgggcgc caacgtgaac ccgcagttgc cggcgacgat gctggtgacg
     1081 ccgtgcagac tcgatgacga ggcgctcggg tcccagaaca gctgggcgtc gtagtgagtg
     1141 tggggatcga cgaaccccgg tgtcaccacc agcccgtcgg cgtcgatggt ctcggccgcg
     1201 gcgtcgtcga ccgtgccgac cgcgacgatg cgcccgtctt gcaccgcgac gtctcctggt
     1261 cgtccgggcg tgccggttcc atccacgacg gtggcgtcct tgatgacgac gtccagcatg
     1321 acgacctctc gacgagaaca ggttctagtt tctcgcgctt cggggacgcg agcaaccgcc
     1381 actttggggc gatccgccca cccgtcgggt gggttggtca ggccgcggac cgcacggcgt
     1441 cacgaccgag gcggcggaga cagcggcggg gtgaagaccc caaccgtaga ctccggccgc
     1501 ggggaggcga ggccctcggc accacgagca ggagcgcacg atggaccacg gcaagaccga
     1561 ggaatggctc cagaaggtgc ccctcttccg ggggctctcc aagaagcagc tccgggacat
     1621 ctcgggtctc gccacccggc tcgacgagcc cgccggcacg gtgttgacca aggaaggcaa
     1681 gatcggtcac gaattcatca tcgtgatcga aggtgagatc gaggtccgca agggcgacca
     1741 ggtcatcgcg accagagggc ccggcgatta cgtgggtgag atcgcgctca tggaccaccg
     1801 tccccgtacc gcgacggtgg tcgccaagac accagtcgtg atcgaggtga tcggccaacg
     1861 cgagttccgg actctgctcg ccgaagcgcc cgggctgtcg agcgacatca tgagcacgat
     1921 ggcgcagcga ctcgccgagc tcgaccaatc gggtgagggg taggccccgt caccggttcc
     1981 ggacctgtgc ccggtcgcgg agctcgtact tcaggacctt tccactggcg ttcagcggca
     2041 gcgcgtccat gacctcgacg tagcgaggaa ccttgtagtt ggccacgtgg tcgcgcgacc
     2101 aggcgatgaa ctcgtcgggg tcgacggtcg cgccgggcct cgccacgacg aacgccatcc
     2161 ccacctcacc aagccggtcg tcgggcaccc cgacgaccgc gacc
//
LOCUS       DATCCH010000122         2846 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_511092, whole genome shotgun sequence.
ACCESSION   DATCCH010000122 DATCCH010000000
VERSION     DATCCH010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511092"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="VIJ44_02445"
     CDS             complement(<1..377)
                     /locus_tag="VIJ44_02445"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEY5164791.1"
                     /translation="MGDATRVGVLGAGVMGSGIAQVTATAGFPTVCYDVDPEALRRGR
                     EHVTTGRYGLERGVERSKLTREQADAALARLTFSDSFEEAAAADLIIEAVPERLALKV
                     SVFRQLDGAAPAGAILASNTSGFP"
     gene            complement(411..1595)
                     /locus_tag="VIJ44_02450"
     CDS             complement(411..1595)
                     /locus_tag="VIJ44_02450"
                     /inference="COORDINATES: protein motif:HMM:TIGR03624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEY5164792.1"
                     /translation="MSGAPQPGEEPMLPFGFGDLDLNQLMRMLQAGGPVNWEIATQVA
                     RHVATDGEPERPDDPAAATQMEELARAAQTHVVAETGLSATFAARVQTTGRTGWVDLH
                     LEALKPVLEALATTLGRALAGSADEPGGPAGTDPFSAMMPMLAPLLLGVQAGSMIGYL
                     AQHALGRYDLPLPTADRPSLCFVIPNVDGFEEAWSLERADLRFTIALHEVVHAAARSV
                     PWVRERLVSLATDYVSAYEVDPVILRARLGDVDPANPEALQSLAERPEELLGAMTSPR
                     QEEMLARLRVFHSVLDGYGDEILARIGARLVPSFARIHEAVTRHRLERGEASRFVEQL
                     LGLTMEREDYERGAAFCAGVVERAGADALNRLWEDPSMIPTASELEAPGLWLARIELD
                     LP"
     gene            1613..2668
                     /locus_tag="VIJ44_02455"
     CDS             1613..2668
                     /locus_tag="VIJ44_02455"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY5164793.1"
                     /translation="MTSIAVTGASGLVGQRLLPVLADDASVTRVVGLDMRPPRRRARG
                     LEFHLVDVARAELKPLLEGIDVLVHLASVVDPIPDQALMARVNVDGTRRVLDAAAAVG
                     VHRVVRVSSATVYGAWANNPVPLTEDSPLRPNPGFSPAVQAAEVERLLAEWRDDHPGV
                     VVTTLRAAPVLGPGAERLPSRILLGRPPLRVRHATPPVQAVHVDDLVAALAIVVLSDH
                     PGTFNVASDGWLDHDQARRLLEPSRIPPLPSGLLERVLRVTWDIGIGDVPPGVLPYLV
                     HPLVIANDRLRALGWVPRHDNEQAVVDGLDSFGPRRSKVWFALGGGAAAGAAATAWLL
                     GRRWRRGARRATRPSQT"
     gene            complement(2598..>2846)
                     /locus_tag="VIJ44_02460"
     CDS             complement(2598..>2846)
                     /locus_tag="VIJ44_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018672771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY5164794.1"
                     /translation="HCYRRPTYPDWPYSVFTMVHGKHSRDCETTIAAIREETGVDDYA
                     LLWSIKEYKKTRVRYFTSDWDEWRAGHLAASGAPTATP"
BASE COUNT          370 a         1044 c          999 g          433 t
ORIGIN      
        1 gggaaccccg aggtgttcga cgccaggatc gctcccgcgg gcgcggcccc gtcgagctgg
       61 cggaacaccg agaccttcaa ggccagtcgt tcggggacgg cctcgatgat caggtccgcg
      121 gccgcggcct cctcgaagga atcggagaac gtgagccggg ccagcgccgc gtcggcctgc
      181 tctcgggtca gcttcgaccg ctccacgcca cgctcgagcc cgtagcggcc ggtcgtgacg
      241 tgctcgcggc ctcgccggag cgcctcgggg tcgacgtcgt agcagacggt cgggaacccc
      301 gccgtggcgg tcacctgcgc gataccgctc cccatcaccc cggcgccgag gaccccgact
      361 cgggtcgcgt cacccaacgg gttcctccgt cggtctgggc tcggcggcgt tcacggcagg
      421 tcgagctcga tgcgggctag ccacagcccc ggcgcctcga gctccgaagc cgtcgggatc
      481 atgctcggat cctcccagag ccggttcagc gcgtcggccc cggcccgctc cacgacaccg
      541 gcgcagaagg ccgcccctcg ctcgtagtcc tctcgctcca tcgtgaggcc caacagctgt
      601 tccacgaagc ggctcgcctc cccccgctcc aaccggtgtc gggtcacggc ctcgtggatc
      661 cgagcgaacg acgggaccag ccgcgccccg atgcgggcca ggatctcgtc gccgtacccg
      721 tcgagcaccg agtgaaagac ccgcagccgg gccagcatct cctcttgacg cggtgacgtc
      781 atcgcgccga gcagctcctc gggacgctcg gcgaggctct gcagcgcctc ggggttcgcc
      841 gggtcgacgt cgccgagccg ggcccgcaag atcaccgggt ccacctcgta ggccgacacg
      901 tagtcggtgg cgagactcac gagacgttcc cgcacccacg gcaccgaacg ggccgcggcg
      961 tgcacgacct cgtgcaacgc gatcgtgaac cgcaggtcgg cgcgctcgag tgaccaggcc
     1021 tcctcgaacc catcgacgtt cgggatgacg aagcacagcg agggtcgatc cgcggtcggc
     1081 agcggcaggt cgtaccggcc cagggcgtgc tgggcgaggt agccgatcat cgatccggcc
     1141 tgcaccccga ggagcaacgg cgccagcatc ggcatcatcg ccgagaacgg gtcggtcccg
     1201 gcggggccgc cgggttcgtc ggccgaaccc gccagcgccc ggcccagcgt ggtggccagc
     1261 gcctcgagca ccggcttgag ggcctcgagg tgcaggtcga cccaaccggt gcggcccgtg
     1321 gtctggaccc gggcggcgaa cgtggcgctg agcccggtct cggcgacgac atgggtctgc
     1381 gcggcgcggg cgagctcctc catctgggtc gcggccgcgg ggtcgtcggg acgttccggc
     1441 tcgccatcgg tcgcgacgtg gcgggcgact tgggtcgcga tctcccagtt gacggggccc
     1501 cccgcctgca gcatccgcat gagctgattt aggtcgaggt caccaaaccc gaacggcagc
     1561 atcggctcct cgccgggttg aggggcgccg ctcacgcggc cagtctatga acgtgacttc
     1621 gatcgccgtc accggggcat ccgggctcgt cggccagcgg ctcttgcccg tgttggccga
     1681 cgacgcgtcg gtcacgcgtg tcgtcgggct cgacatgcgt cccccgcgtc gacgggcgcg
     1741 gggcctcgag ttccatctcg tcgacgtggc ccgagccgag ctcaagccgc tgctcgaggg
     1801 catcgacgtg ctcgtccatc tcgcctcggt tgtcgatccg atcccggacc aggccctgat
     1861 ggcccgcgtg aacgtcgacg ggacccgtcg cgtcctggac gcggccgcgg cggtgggtgt
     1921 ccaccgggtc gtgcgcgtct cgagcgccac ggtctacggc gcgtgggcga acaatccggt
     1981 gccgctcacc gaagattctc cgttgcgccc gaacccgggg ttctcgccgg ccgtgcaggc
     2041 cgccgaggtc gagcggctcc tcgccgagtg gcgcgacgac catcccggtg tcgtggtgac
     2101 gacgctgcgc gccgcgccgg ttctcgggcc cggcgccgag cggctcccgt ctcggatcct
     2161 gttgggccga cccccgttgc gggtccgcca cgcgacaccc ccggtccagg ccgtgcatgt
     2221 cgacgacctg gtcgcggcgt tggcgatcgt cgtgctcagc gaccaccccg gcaccttcaa
     2281 cgtcgcctcg gacggctggc tcgaccacga ccaggcccgg cggctgctcg agccgtcccg
     2341 aatcccgccg ctcccgtcgg gtctgctcga acgggtgctg cgcgtcacgt gggacatcgg
     2401 gatcggtgac gtcccgcccg gtgttctgcc gtacctcgtg cacccgttgg tgatcgccaa
     2461 cgaccggctg cgggccctcg ggtgggtgcc ccgtcacgac aacgagcagg ccgttgtgga
     2521 cgggctcgac tcgttcgggc ctcggcggtc gaaggtctgg ttcgcgctcg gcggtggtgc
     2581 ggccgccggc gccgccgcta cggcgtggct gttggggcgc cgctggcggc gaggtgcccg
     2641 gcgcgccact cgtcccagtc agacgtgaag tagcggacac gcgtcttctt gtactccttg
     2701 atcgaccaga gcagcgcgta gtcgtccacg ccggtctcct cgcgaatcgc cgcgatggtc
     2761 gtctcacagt cccgactgtg tttgccgtgc accatcgtga acaccgagta cggccagtcg
     2821 gggtaggtcg gccgccggta gcagtg
//
LOCUS       DATCCH010000123         2355 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_587300, whole genome shotgun sequence.
ACCESSION   DATCCH010000123 DATCCH010000000
VERSION     DATCCH010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587300"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VIJ44_02465"
     CDS             <1..387
                     /locus_tag="VIJ44_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3151 family protein"
                     /protein_id="HEY5164795.1"
                     /translation="DDALAALVAAWGQPPLARHDAIAAVVAHWPTFLDGWARLGDAAR
                     DDVEAYACYRVGYHRGLDRLRRAGWRGSGYVRWVHPTNRGFLRALAGLGRVAAAIGED
                     DEADRCFTFLHQLEPDWDRVASEPGQ"
     gene            398..1390
                     /locus_tag="VIJ44_02470"
     CDS             398..1390
                     /locus_tag="VIJ44_02470"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006238376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042351 - 'de novo' GDP-L-fucose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEY5164796.1"
                     /translation="MRRALITGITGQDGRHMSEFLTGKGYQVFGLVRGQNNPKAQLVQ
                     DENPSLELVEGDLRDLSSLIAALEQVQPDEVYNLGAMSFVALSFRQPELTADTTGLGV
                     LRMLEAIRIVGGTENNPIRFYQASSSEMFGKVRETPQTEETPFHPRSPYGVAKVFGHS
                     ITVNYRESYGMHASSGICFNHESPRRGLEFVTRKITNGLARIKLGLQDSITLGNLDAA
                     RDWGYAGDFVEAMWLMVQQDDPGDYVIATGETHTIREFLDLAFRCAEIDGWEDRIRTD
                     PRFERPAEVDVLTGDATKALEQLGWKPRVTFEELVRMMYEADLVEEAARAEIAR"
     gene            complement(1391..>2355)
                     /locus_tag="VIJ44_02475"
     CDS             complement(1391..>2355)
                     /locus_tag="VIJ44_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007072754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5164797.1"
                     /translation="FGVQTLDDGLAVRAASDADDARHAVVVGGGYVGLELGEALVRRG
                     LSVALVESAPQPMSTLDPEMGALVADALRSVGVEVYVGERVEGFDTDAGQVTGVQTAA
                     RVLPADLVILGLGVDPNSGLARDAGIPVGASGGVAVDDHVRAGVDGIYAAGDCSETFH
                     RVSRRPVVVALGTHANKQGRVAGINASGGDAVFPGVLGTAASKICKYEVARTGLNERE
                     AAAAGFDAFAVSTDTTTRAGYYPDAAPIRVRVVAERGTGRLLGAQIVGEEGAAKRVDV
                     LAAAIWNEMTVDEVASMDLSYAPPFAPVWDPTLMAARRAASALA"
BASE COUNT          367 a          854 c          802 g          332 t
ORIGIN      
        1 gacgacgccc tcgccgcgct cgtcgccgcc tggggccagc cgcccctggc ccggcacgac
       61 gcgatcgccg cggtcgtcgc ccactggccc accttcttgg acgggtgggc tcgtctcggc
      121 gacgcggcac gcgacgacgt cgaggcgtac gcctgctatc gggtcggcta ccaccggggc
      181 ctcgaccggc tccgccgggc cggctggcgc gggtcgggct acgtccggtg ggtgcacccg
      241 accaaccggg ggttcctccg ggcccttgcc gggctgggcc gggtggccgc ggcgatcggc
      301 gaggacgacg aagccgaccg gtgcttcacc ttcctccacc agctcgagcc ggactgggat
      361 cgggtcgcct cggagcccgg ccagtaacgt cggcgtcgtg cgacgagcgc tcatcaccgg
      421 aatcacgggc caggacggcc ggcacatgtc ggagttcctg accggcaagg gctatcaggt
      481 cttcggtctg gtccggggcc agaacaaccc gaaagcccaa ctggtccagg acgagaaccc
      541 gtcgctggag ctcgtcgagg gagacctccg ggacctgtcc agcctcatcg ccgcgctcga
      601 gcaggtccag cccgatgagg tctacaacct cggcgcgatg agcttcgtgg ccctgtcgtt
      661 ccgccagccg gaactgaccg ccgacaccac cggcctcggg gtgctgcgaa tgctggaggc
      721 gatccggatc gtcggaggca ccgagaacaa cccgatccgc ttctaccagg cgtcatcgtc
      781 ggagatgttc gggaaggtgc gagagacccc ccagaccgag gagaccccgt tccatccccg
      841 ctccccgtac ggggtggcga aggtcttcgg tcactcgatc accgtgaact accgggagtc
      901 ctacgggatg cacgcctcgt cgggcatctg tttcaaccac gagtcgccac ggcgggggct
      961 cgagttcgtg acccgcaaga tcaccaacgg cttggcccgg atcaagctcg gtctccagga
     1021 ttccatcacc ctcgggaatc tcgacgctgc acgcgactgg ggttacgccg gcgacttcgt
     1081 cgaggcgatg tggctcatgg tgcaacaaga cgaccccggc gactacgtga tcgccaccgg
     1141 ggagacccac acgatccgcg agttcctcga cttggcgttc cgctgcgccg agatcgacgg
     1201 ctgggaggac cgcatccgaa ccgatccacg gttcgagcgt ccggcggagg tcgacgtcct
     1261 cactggtgac gccacgaagg ccctcgagca gctgggttgg aagccccgcg tcacgttcga
     1321 ggagctggta cggatgatgt acgaggccga cctcgtcgaa gaggccgccc gggccgagat
     1381 cgcgcggtag ttacgcgagg gccgaggcgg cgcgccgcgc cgccatcagt gtcggatccc
     1441 acaccggagc gaacggcggc gcgtacgaca ggtccatcga cgcgacctcg tcgaccgtca
     1501 tctcgttcca gatcgccgcg gcgagaacgt cgacgcgttt cgcggcccct tcctcaccca
     1561 cgatctgggc accgaggagt cgcccggtcc cccgttcggc gacgacccgg acccggatcg
     1621 gggccgcgtc ggggtagtac ccggcccggg tggtggtatc ggtcgacacc gcgaacgcgt
     1681 cgaacccggc cgcggcggct tcgcgctcgt tgagaccggt gcgggccacc tcgtacttgc
     1741 agatcttgga cgccgcggtc ccgagcaccc cggggaacac cgcgtcgcct ccgctggcgt
     1801 tgataccagc gacgcggcct tgcttgttgg cgtgggtccc gagcgccacg acgacaggcc
     1861 gacgggagac ccgatggaac gtctcggagc agtcgccggc ggcgtagatc ccgtcgacgc
     1921 cggcacgaac atggtcgtcg acggcgaccc cgccgctcgc acccaccggg atccccgcgt
     1981 cgcgggcgag gccgctgttg ggatcgacgc ccagaccgag gatcacaagg tcggcgggca
     2041 acacccgcgc cgcggtctgc accccggtga cctggccggc gtcggtgtcg aacccctcga
     2101 cgcgctcgcc gacatagacc tcgacgccga cggatcgcag cgcgtccgcg acgagcgccc
     2161 ccatctccgg gtcgagggtg ctcatgggct gcggcgccga ttccaccagc gcgaccgaga
     2221 ggccccgccg gaccagcgcc tctcccagct ccagtccgac gtagccaccc ccgacgacga
     2281 ccgcgtgacg ggcatcgtcg gcgtcggacg cggcccgaac cgcgagcccg tcgtcgaggg
     2341 tctgcacccc gaaga
//
LOCUS       DATCCH010000124         3816 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_338903, whole genome shotgun sequence.
ACCESSION   DATCCH010000124 DATCCH010000000
VERSION     DATCCH010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338903"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1032)
                     /gene="hemW"
                     /locus_tag="VIJ44_02480"
     CDS             complement(<1..1032)
                     /gene="hemW"
                     /locus_tag="VIJ44_02480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEY5164798.1"
                     /translation="MALGVYVHIPFCASKCDYCDFATWTDRAALIDDYVDACVTDLRA
                     RVRSGDLDGATSVFVGGGTPSLLPADALTRILDEIPRAPDAEVTVECNPESVDGAKLG
                     RYRTAGVNRCSFGVQSFRPHVLAALGRHHDVAAVTRAVDAARECGFETFNLDLIYGTV
                     GESIDDWRATLDAALDLDPPHVSAYALTVEPATALGRRVAAGTAPAPDDDDQAHKYEL
                     TDDILSAHGLDWYEVSNWARPGHECRHHLLYWDQGDYVAIGCAAHAHRDGHRTWNVRT
                     PERYIERIRTGAAPEADGEHLDAATRTDEAFALALRTRRGAVPSPGAETEVDALTAAG
                     LLIRDGDRVL"
     gene            1139..2275
                     /locus_tag="VIJ44_02485"
     CDS             1139..2275
                     /locus_tag="VIJ44_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY5164799.1"
                     /translation="MNPDPGAARRDVRAFLAEQAPNGRYPRDFVRRLAGAGYVAPHWP
                     VPWGRAANPAEQLAIDEELRAAAAPRPLNPIGIGWAGPTLLVAGTDEQQRRWLPGILD
                     GSELWCQLFSEPEAGSDLASLTTRAERDGDEYVVHGHKVWTTIAHLARWGILLARTDP
                     DRPRRRGISYFVLDMKSAGVTVRPIRQMTGASDFNEVILDGVRVPVDHRVGDDGDGWG
                     LARVTLGNERVSLSSEGALWGLGPTMHDVVELVRAHGGTDDAPRRQRLAAHLTEAEVL
                     RLIRLRTVAAQVRGLEPGPETSVRKTLADQHGQHVMGDAVELAGSYGMLRDAGPFGEP
                     GDEWATGYLFSPALTIGGGTSEVQRGIIAERLLGLPRDAEAPER"
     gene            complement(2286..2609)
                     /locus_tag="VIJ44_02490"
     CDS             complement(2286..2609)
                     /locus_tag="VIJ44_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164800.1"
                     /translation="MSLTPAEVRERVLIWFRPYSHRVVVDTLDRLEVVSGSQAKLRII
                     GGAFIAASSLPTRTAVTIDAGAAGSKVGVTSRDAVGFGLKTGMKHKYEQWVASIVDGI
                     RAALT"
     gene            complement(2677..>3816)
                     /gene="lepA"
                     /locus_tag="VIJ44_02495"
     CDS             complement(2677..>3816)
                     /gene="lepA"
                     /locus_tag="VIJ44_02495"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEY5164801.1"
                     /translation="VTGARLRFVQANATHDAEEVGVRSPAPTPVASLGPGEVGYLIAG
                     IKEVRQARVGETVTTVAHPGAVLPGYRDPKPMVFCGLYPVVGDEYADLREALEKLLLN
                     DSSFTYEPETSGALGFGFRCGFLGLLHMEIVRERLEREYDLSLVATAPNVEYRAHRSD
                     GTVELVDNPSALPPANEIEQIEEPYVTVTILTPTDYVGPLMDLAQSRRGDMTKMEYLS
                     EERVELVYTLPLAEIVMDFFDVLKSRTRGYASLDYEPAGYRPSNLVRVDVLLNGIPVD
                     AFSTIVHRQKAQDYGRRMTDKLSELIPRQLFDVPIQAAIGGRVIARETVKAKRKDVLA
                     KCYGGDVTRKRKLLERQKEGKRRMKQIGRVEVPQEAFVAALRLDN"
BASE COUNT          533 a         1217 c         1496 g          570 t
ORIGIN      
        1 caagacccgg tccccgtcgc ggatgagcag cccggcggcg gtgagggcgt cgacctcggt
       61 ctcggcgccg ggggacggga cggcgccgcg ccgggtgcgg agcgcgaggg cgaacgcctc
      121 gtcggtccgg gtggcggcgt cgaggtgttc cccgtcggct tcgggggccg cgccggtgcg
      181 gatccgttcg atgtagcgct cgggggtgcg gacgttccag gtgcggtgac cgtcgcggtg
      241 ggcgtgggcg gcgcacccga tcgcgacgta gtcgccctgg tcccagtaga gaaggtggtg
      301 gcggcactcg tggcccggtc gggcccagtt cgacacctca taccagtcga ggccgtgcgc
      361 ggagaggatg tcgtcggtca gctcgtactt gtgggcctga tcgtcgtcgt cgggggcggg
      421 ggcggtgccc gccgcgacac gtcggcccag tgccgtggcc ggctcgacgg tgagcgcata
      481 cgcgctcacg tgcggcgggt cgaggtcgag cgcggcatcg agggtggccc gccagtcgtc
      541 gatcgactcc cccaccgtcc cgtagatcag gtcgaggttg aacgtctcga acccgcattc
      601 gcgggccgcg tcgacggcgc gggtgaccgc cgcgacgtcg tggtgtcggc cgagcgcggc
      661 gaggacgtgg gggcggaagg actgcacccc gaacgagcag cggttcaccc ccgcggtgcg
      721 gtagcggccg agcttggccc cgtcgaccga ttcggggttg cattcaacgg tgacttcagc
      781 gtcgggagcg cgggggatct cgtcgaggat gcgggtgagc gcatcggcgg ggagcaggga
      841 tggggtgcca ccgccgacga agacgctggt ggcgccgtcg aggtccccgg agcggacccg
      901 ggcccgaagg tcggtgacgc aggcgtcgac gtagtcgtcg atgagcgccg cccggtcggt
      961 ccaggtggcg aagtcgcagt agtcgcactt cgacgcgcag aacgggatgt gcacgtacac
     1021 gccgagcgcc acaccccatc ctcgcgccac tgaccggccg ggcgacgagg acggccgggc
     1081 cgcgggggac accggttcgg gggcgacggg cggtggggca cggcgcgagg ctggcggggt
     1141 gaaccccgat ccgggtgcgg cccggcgcga cgtgcgcgcc ttcctcgccg agcaggcgcc
     1201 gaacggccgg tacccgaggg acttcgtgcg ccgcctcgcg ggtgcggggt acgtcgcgcc
     1261 gcactggccg gtgccgtggg gacgagccgc caacccggcc gagcagctgg cgatcgacga
     1321 ggagcttcgg gccgcggccg ctccccggcc gctgaacccg atcgggatcg gctgggccgg
     1381 tccgaccctg ctggtcgcgg gcaccgatga gcagcagcgg cgttggctgc ccgggatcct
     1441 cgacgggtca gagctctggt gtcagctctt cagcgagcct gaggccggga gcgatctggc
     1501 gtcgctgacg acgcgggcgg aacgcgacgg tgacgagtac gtcgtgcacg gccacaaggt
     1561 gtggacgacg atcgcgcacc tggcccgttg ggggatcctc ctggcccgca ccgaccccga
     1621 ccggccgcgc cggcggggca tctcgtactt cgtgctcgac atgaagtcgg cgggggtgac
     1681 ggtgcggccg atccgccaga tgacgggggc gagcgacttc aacgaggtga tcctcgacgg
     1741 cgttcgggtg ccggtcgacc atcgggtggg cgacgacggc gacggatggg ggctagcccg
     1801 ggtgacgctc gggaacgagc gggtgtcgct gtcgagcgaa ggggcgttgt ggggtctggg
     1861 tccgacgatg cacgacgtcg tcgagctggt ccgggcccac ggcgggaccg acgacgcgcc
     1921 gcggcgccag cgcctcgcgg cgcatctcac cgaggccgag gtgctgcgcc tgatccggtt
     1981 gcggacggta gcggcccaag tgcggggcct ggagccgggc ccggagacgt cggtgcgcaa
     2041 gaccctcgcg gaccaacacg gccagcacgt catgggtgac gcggtcgagt tggccggctc
     2101 gtacgggatg ctgcgcgacg cgggaccgtt cggtgagccc ggggacgagt gggccacggg
     2161 gtacctgttc tcgcccgcgt tgacgatcgg gggcggaacc agcgaggtgc agcgcggcat
     2221 catcgccgag cgactcctcg gactgccccg cgacgccgag gcaccggagc gctgactcgg
     2281 ctcgctcagg tgagcgcggc gcggatccca tcgacgatcg acgccaccca ctgctcgtac
     2341 ttgtgcttca tgccggtctt gagaccgaag ccaaccgcgt ctcgggaggt gacaccaacc
     2401 ttggatccgg cggcgccggc gtcgatggtg acggcggtgc gggtcgggag cgacgacgcg
     2461 gcgatgaacg ccccgccgat gatccgcagc ttggcctggg atccgctgac cacctcgagc
     2521 cggtcgaggg tgtcgacgac gacccggtgg gagtagggac ggaaccagat cagcacccgt
     2581 tcgcgcacct ccgcgggggt gagcgacacg tcgaaggtgt cggtgtgggt caggggtcct
     2641 atcgggccga gcctacggcg acgacgcggg ccgggatcag ttgtcgaggc ggagcgcggc
     2701 gacgaacgcc tcttggggga cctcgacgcg gccgatctgc ttcatgcgcc gcttcccctc
     2761 tttctggcgt tcgaggagtt tgcgtttgcg ggtgacgtca ccgccgtagc acttggcgag
     2821 cacgtccttg cgtttggctt tcaccgtctc gcgggcgatg acccgtcccc cgatggcggc
     2881 ttggatcggg acgtcgaaga gctgtcgggg gatgagctcg ctgagcttgt cagtcatgcg
     2941 gcgtccgtag tcctgggcct tctggcggtg cacgatggtc gagaaggcgt cgacggggat
     3001 cccgttgagc aacacgtcga ctcgcaccaa gttcgagggt cggtagccgg ccggctcgta
     3061 gtcgaggctg gcgtagcccc gggtgcggga cttcaggacg tcgaagaagt ccatgacgat
     3121 ctcggccagc ggcagggtgt agacgagctc gacgcgttcc tcggacaggt actccatctt
     3181 cgtcatgtcg cctcggcggg actgggccag gtccatgagc ggcccgacgt agtcggtcgg
     3241 ggtgaggatg gtgacggtga cgtacggctc ttcgatctgt tcgatctcgt tcgccggtgg
     3301 cagcgccgac gggttgtcga cgagctccac ggtgccgtcg ctgcgatgcg cccggtactc
     3361 gacgttcggg gcggtggcga ccaaggacag gtcgtactcg cgttcgagtc gctcccggac
     3421 gatctccatg tgcaacaggc cgaggaaccc gcagcggaac ccgaacccga gcgccccgga
     3481 agtctcgggt tcgtaggtga acgacgaatc gttgagcagg agcttctcga gcgcctcgcg
     3541 caagtcggcg tactcgtcgc cgacgacggg atacaggccg cagaacacca tgggcttcgg
     3601 gtcgcggtac ccggggagca ccgcgcccgg gtgcgcgacg gtggtgacgg tctcaccgac
     3661 ccgggcttgg cggacctcct tgatgccggc gatcaggtag ccgacctcac cggggccgag
     3721 ggacgccacc ggcgtcgggg ccgggctgcg aaccccgacc tcttcggcgt cgtgggtggc
     3781 gttggcttgc acgaaccgca gacgggcgcc ggtgac
//
LOCUS       DATCCH010000125         2036 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_380477, whole genome shotgun sequence.
ACCESSION   DATCCH010000125 DATCCH010000000
VERSION     DATCCH010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380477"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="VIJ44_02500"
     CDS             complement(<1..706)
                     /locus_tag="VIJ44_02500"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEY5164802.1"
                     /translation="MSSLNLDRLLDAAVAAARAGGEIVRGSVDTAPEAREKAPGDWVS
                     DADTASERAVRSVLAAAAPDLPVHGEEEGGERGRIGWLVDPIDGTTNFLHRFPVVGVS
                     VALVDDGQPIVGVVHAPLLGDTYTARVGGGAHRNGTPIRVSERTPSQSVLATGTPFRA
                     KQTRLREYVRVFERAMLAFEDLRRAGAASLDLAWTAAGVFDGYFEQALGPWDVAAGAL
                     LVREAGGLVTDWAGDRD"
     gene            complement(703..1449)
                     /locus_tag="VIJ44_02505"
     CDS             complement(703..1449)
                     /locus_tag="VIJ44_02505"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEY5164803.1"
                     /translation="MGPADLLDLFVTAADAQRVALAPLSATELRTRTPRPGQYALDLV
                     ADAAVLDVLHRAPVAIVSEESGRSGPAAAAITVVVDPVDGSTNCSRRIPYWGISLCAL
                     DADGPLCALVVNTTTGEQFSAVRGDGAQVDGTPLRASTTTRVEDSFVAVSSLPRPQPW
                     KQFRALGSTALALCDVAAGRVDAYLDCPGFEQAPWDYLGGLLACTESGATVVDARDRA
                     LAVIDPDARRQVLAAATPVLLDALRAGVAP"
     gene            complement(1481..>2036)
                     /locus_tag="VIJ44_02510"
     CDS             complement(1481..>2036)
                     /locus_tag="VIJ44_02510"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEY5164804.1"
                     /translation="QRVDPTRADLIRFESGDEIRADIAATIPAYAGIERLHQTGDQVQ
                     WGGRHLCADGRFPTADGRASFTVVEPPAVGPSDGRLRLATRRGKQFNTMVMGERDPLT
                     GATRDAVFVAADDAARLGVHDGDPVVVRSDHGQLHGRVRISALRAGNVQVFFPEGNVL
                     LPMGRRDESGVPDYTAPVEVVPAR"
BASE COUNT          275 a          720 c          775 g          266 t
ORIGIN      
        1 cgtcgcggtc ccccgcccag tcggtgacca gccctccggc ttctcggacc agcagcgcgc
       61 cggcggcgac gtcccacggg ccgagggcct gttcgaagta gccgtcgaag accccggccg
      121 cggtccaggc gaggtcgagg ctggcggcgc cggcccgtcg gaggtcctcg aaggccagca
      181 tggcccgctc gaacacgcgc acatactcgc ggaggcgcgt ctgcttggcc cgaaacgggg
      241 tcccggtggc gagcaccgac tgcgatggtg tgcgctcact cactcggatg ggggtgccgt
      301 tgcggtgggc tccgccaccc acccgagcgg tgtaggtgtc gccgagcagc ggggcgtgga
      361 cgacaccgac gatcggctgt ccgtcgtcga cgagcgcgac ggacacgccg acgaccggga
      421 agcggtgcaa gaagttggtg gtgccgtcga tcgggtcgac cagccagccg attcggcctc
      481 gctccccacc ttcttcttcg ccgtggacgg gtaggtccgg ggccgcggcg gcgagcaccg
      541 accgcaccgc ccgttcgctg gcggtgtcgg cgtcgctgac ccagtccccc ggggccttct
      601 cccgggcttc gggtgcggtg tcgacggagc cgcgcacgat ctcgccgccg gcgcgggcgg
      661 ccgcgaccgc ggcgtcgagg agccggtcga ggttgaggct gctcatggcg ccacgccggc
      721 gcggagcgcg tcgaggagca caggcgtcgc ggcggccagc acctggcggc gcgcgtccgg
      781 gtcgattacg gccagcgccc ggtcccgggc gtcgacgacg gtcgcaccgg attcggtgca
      841 ggccagcaac ccgccgaggt agtcccaggg ggcttgctcg aacccggggc agtcgaggta
      901 ggcgtcgacc cgaccggcgg cgacatcgca cagcgcgagc gcggtggatc cgagcgcccg
      961 gaactgcttc cacggttgtg gccggggcag cgacgacacc gcgacgaagg agtcctcgac
     1021 tcgggtggtc gtcgaggccc gcaacggcgt gccgtcgacc tgggccccgt caccgcgcac
     1081 cgcggagaac tgctccccgg tcgtggtgtt gaccaccaga gcacagaggg gaccgtccgc
     1141 gtcgagcgcg cagagcgaga tcccccagta ggggatgcgc cgcgagcagt tcgtggatcc
     1201 gtcaaccggg tcgacgacca cggtgatcgc agccgccgcg ggtcccgacc ggccggattc
     1261 ctcgctcacg atggcgaccg gggcgcggtg caggacgtcg aggacggccg cgtccgcgac
     1321 gaggtcgagc gcgtactggc ccggtcgggg ggttcgcgtc cgcagctcgg ttgcgctcag
     1381 gggggcgagc gcgacgcgtt gcgcgtccgc ggcggtgacg aagaggtcga ggaggtcggc
     1441 ggggcccacg gcccggcagt ctcgcagact gcctcgcagc tcagcgcgct gggacgacct
     1501 cgaccggggc ggtgtagtcg ggcacgccgg actcgtcgcg tcggcccatg gggagcaaga
     1561 cgttcccttc ggggaagaac acctgcacgt tcccggcccg cagcgcactg atccggaccc
     1621 gtccgtgcag ctgcccgtga tccgatcgga ccacgaccgg gtcgccgtcg tggacaccga
     1681 gccgggcggc atcatcggcg gcgacgaaca ccgcgtcccg ggtggcaccg gtgagggggt
     1741 cccgctcccc catcaccatg gtgttgaact gcttgccccg ccgggtcgcc aaccggagcc
     1801 gaccgtcgct cggccccaca gccgggggtt ccaccacggt gaacgacgcc cggccgtcgg
     1861 cggtcgggaa ccgaccgtcg gcgcagaggt gccgcccacc ccattggacc tggtcgccgg
     1921 tctggtgcag ccgctcgatc ccggcgtacg ccgggatggt cgcggcgatg tcggcccgga
     1981 tctcgtcccc ggattcgaag cgaatcaggt cggcgcgggt ggggtcgacg cgctgg
//
LOCUS       DATCCH010000126         3537 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_421523, whole genome shotgun sequence.
ACCESSION   DATCCH010000126 DATCCH010000000
VERSION     DATCCH010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421523"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2523
                     /gene="secA"
                     /locus_tag="VIJ44_02515"
     CDS             <1..2523
                     /gene="secA"
                     /locus_tag="VIJ44_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEY5164805.1"
                     /translation="ETARRTLGQRHFDVQLMGGAALHFGWVAEMKTGEGKTLVATLPV
                     YLNALAGYGVHVVTVNDYLARRDADWMGQIYRSLGLDVGVVIPEVFDYEAKRTAYRAD
                     VTYGTNNEFGFDYLRDNMTGSGEDQVQRGHTFAIVDEVDSILIDEARTPLIISGPADD
                     AQQLYNQFAQIVKPLQRDRDYEVDEAKKTVVPTEEGISRVEQALDISNLYEHVNQNFV
                     HQLQAALRAKELFKRDVDYVIQGGEIKIVDEFTGRILEGRRWSEGLHQAVEAKEAVRI
                     KEENQTLATVTLQNYFRLYDKLAGMTGTASTEAGEFAHTYGLEVVSIPTNQPMVRADQ
                     PDLIYKSEGAKFEAVADDIQDRREAGQPVLVGTISVEKSELLSSILDRRGITHSVLNA
                     KQHEREAHIVTQAGQLGGVTVATNMAGRGVDILLGGNPDGLARRDLLAEGTTPEDSPE
                     RFAELLARHTAQCAAEGDRVRELGGLYVLGTERHESRRIDNQLRGRSGRQGDPGESRF
                     YLSLEDDLMRLFATGAMSWVMDRAFPDDMPLEARMVTKAIERAQHTVEDRNFEIRKDV
                     LKYDEVMNEQRKIIYRRRQQILNGEDLREPTIESIQDSIERLVTAFCPGEFPEDWDLD
                     EFHRNLQVTLPSEVTRPELDDCIHLDQLRETVLSEALTKYEAKEESIGKETLREIERR
                     VMLSVIDQRWREHLYEMDYLREGINLRAMGQQDPLAEWQREGYDMFEAMMAEIEDDFV
                     RYVTHLQVVTEDAPDPLASSLQYSAAEDPVQGSEALRSALATPVSSEPQPTADALPPD
                     PEAFDPYDPELQQEPVRVEKTPGRNEPCFCGSGKKYKLCHGR"
     gene            2554..>3537
                     /gene="prfB"
                     /locus_tag="VIJ44_02520"
     CDS             2554..>3537
                     /gene="prfB"
                     /locus_tag="VIJ44_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153759895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEY5164806.1"
                     /translation="MRDSTDALADARRRVEDARAYLRVDAARDRLAELEQALASEPDL
                     WDDPDRARDLTKQHARAREDVELIDGLDRRISDLETLHQLAREEGDESLEPEIETGLG
                     TLVDELDRLELRALFTGDHDERDAICEIHSGAGGTDAQDWAHMLFRMFTRWAERRGFD
                     VEVDEVLPGTEAGLTSATFTVKGRYAYGMLEGERGVHRLIRISPFDANARRQTAFASL
                     DGVPALEATEEPDIDPGDLRIDTFRSSGAGGQHVNVTDSAVRITHVPSGIVVSCQNER
                     SQAQNKARAMQILAARLAERQRQDREKELERLSGDKRDVAFGSQIRTYTLAP"
BASE COUNT          673 a         1275 c         1108 g          481 t
ORIGIN      
        1 gagaccgccc gacgcaccct cgggcagcgt catttcgacg ttcagttgat ggggggcgcg
       61 gcgctgcact tcgggtgggt cgccgagatg aagaccggcg aaggcaagac cctcgtcgcg
      121 accctgccgg tctacctcaa cgcgctggcc ggctacggcg tccatgtcgt caccgtgaac
      181 gactacctcg cccgccgcga cgcggactgg atgggtcaga tctaccggtc tctcggtctc
      241 gacgtcggcg tcgtcatccc cgaggtcttc gactacgagg cgaagcgaac ggcgtaccgg
      301 gctgacgtca cctacgggac caacaacgag ttcgggttcg actacctgcg cgacaacatg
      361 accggctcag gcgaggacca ggtgcagcgc gggcacacgt tcgccatcgt cgacgaggtc
      421 gactcgatcc tcatcgacga ggcccgcaca ccgctgatca tctcgggccc ggccgacgac
      481 gcgcagcagc tctacaacca gttcgcgcag atcgtgaagc ccttgcaacg tgaccgggac
      541 tacgaggtcg acgaggcgaa gaagaccgtc gtgcccaccg aggagggcat cagtcgggtc
      601 gagcaggcgc tcgacatctc gaacctctac gagcacgtga accagaactt cgtccatcag
      661 ctccaggccg cgctgcgagc caaggagctc ttcaagcgcg acgtcgacta cgtcatccaa
      721 ggcggcgaga tcaagatcgt cgacgagttc accggccgga tcctcgaggg ccgacgctgg
      781 agcgaaggtc tgcaccaggc cgtcgaggcc aaggaagccg tgcgaatcaa agaggagaac
      841 cagaccctcg ccacggtcac gctccagaac tacttccgcc tgtacgacaa gctggccggg
      901 atgaccggca ccgcctccac cgaggcgggc gagttcgccc acacctacgg gctcgaggtc
      961 gtctccatcc cgacgaacca gccgatggtc cgcgccgacc agcccgatct catctacaag
     1021 tccgagggcg cgaagttcga agccgtcgcc gacgacatcc aggatcgccg cgaggccggc
     1081 cagccggtgc tcgtcggcac catctcggtc gaaaagtccg agctgctgtc gagcatcctg
     1141 gaccgccgcg gcatcacgca ctcggtgctc aacgccaagc agcacgagcg ggaagcccac
     1201 atcgtcaccc aggcgggcca gctcggtgga gtcaccgtcg ccaccaacat ggccggtcgc
     1261 ggcgtcgaca tcctcctcgg cggcaacccc gacggtctag cccggcgtga cctgctcgcc
     1321 gaaggcacca cccccgaaga cagccccgag cggttcgccg agctcctcgc ccgccacacc
     1381 gcgcaatgtg ccgccgaagg agaccgcgtc cgcgagctcg gtggcctgta cgtcctcggc
     1441 accgaacgac acgagagccg ccggatcgac aaccagctcc ggggccgctc ggggcgccag
     1501 ggcgaccccg gtgagagtcg cttctacctc tcgctcgagg acgacctcat gcggctcttc
     1561 gcgactggcg cgatgagctg ggtcatggac cgggcgttcc ccgacgacat gccgctcgag
     1621 gctcgcatgg tcaccaaggc catcgaacgg gcccaacaca ccgtggagga ccgcaacttc
     1681 gagatccgca aggacgtcct caagtacgac gaggtgatga acgagcagcg caagatcatc
     1741 tatcggcgcc gccagcagat cctcaacggc gaggatctcc gcgagccgac gatcgagtcg
     1801 atccaggaca gcatcgaacg gctggtcacc gcgttctgcc ccggcgagtt ccccgaggac
     1861 tgggacctcg acgagtttca ccgcaacctg caggtcacct tgccgagcga ggtgacccgg
     1921 cccgagctcg acgactgcat ccacctcgat cagctccgag agaccgtcct cagcgaggcg
     1981 ctgaccaagt acgaggccaa ggaagaatcg atcggcaagg agactctgcg cgagatcgaa
     2041 cgtcgcgtca tgctctcggt gatcgaccag cggtggcgtg agcacctcta cgagatggac
     2101 tacctgcgcg aaggaatcaa cctgcgcgcc atgggccaac aggaccctct cgccgagtgg
     2161 cagcgggagg gctatgacat gttcgaagcg atgatggccg agatcgaaga cgacttcgtc
     2221 cgctacgtca cgcacctcca ggtcgtcacc gaggacgcac cggacccgct ggcaagcagc
     2281 ttgcagtaca gcgccgctga ggacccggtc cagggctccg aggcgctccg cagcgcgctg
     2341 gccacgccgg tctcgtccga gccccagccc accgccgacg cgcttcctcc tgaccccgag
     2401 gcgtttgacc cgtacgaccc cgagctgcaa caagagccag tacgggtcga gaagacgccc
     2461 ggtcggaatg agccctgctt ctgcggcagc ggcaagaagt acaagctctg ccacggccgc
     2521 tgaacgcccg ccgacaccgc tcgccgcttc gccatgcgcg actccaccga tgccctcgcc
     2581 gacgcccgcc gccgcgtcga ggacgcccgc gcctacttgc gggtcgacgc cgcccgggac
     2641 cgcctcgcgg agctcgagca ggccctggcg tccgaacccg acctctggga tgaccccgac
     2701 cgcgcgcgag acctgacgaa gcaacatgcc cgggctcgcg aagacgtcga gctcatcgac
     2761 ggcctcgacc gccgaatctc agacctcgag accctccacc agctcgctcg cgaggagggt
     2821 gacgaatcgc tggagcccga gatcgagacc ggcctcggga ccctcgtcga cgagctcgac
     2881 cgcctcgagc tgcgcgcctt gttcaccggc gaccatgacg agcgcgacgc catctgcgag
     2941 atccattccg gagccggcgg taccgacgcc caagactggg cccacatgct gttccgcatg
     3001 ttcacccggt gggcggagcg ccgcggcttc gacgtcgagg tcgacgaggt cctgcccggc
     3061 accgaggccg gcttgacgtc ggccaccttc accgtcaagg gacgctacgc ctacggcatg
     3121 ctcgaaggag agcgaggcgt gcaccgcctc atccgaatct cgccgttcga cgccaacgcc
     3181 cgccgccaga cggcgttcgc ctccctcgac ggcgttccag cgctcgaagc gaccgaggag
     3241 cccgacatcg accctggtga cctccggatc gacaccttcc ggtcgtcggg tgcggggggc
     3301 cagcacgtca acgtcaccga ctccgcggtg cggatcaccc acgtgccgag cgggatcgtc
     3361 gtgtcctgtc agaacgagcg gtcacaggcc cagaacaagg cccgtgcgat gcagatcctc
     3421 gccgcccggc tggcggagcg gcaacgccag gaccgcgaga aggagctgga acgtctctcc
     3481 ggcgacaaac gcgacgtcgc cttcggcagc cagatccgca cgtacacgct ggcgccg
//
LOCUS       DATCCH010000127         4117 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_470515, whole genome shotgun sequence.
ACCESSION   DATCCH010000127 DATCCH010000000
VERSION     DATCCH010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4117
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470515"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1209
                     /locus_tag="VIJ44_02525"
     CDS             <1..1209
                     /locus_tag="VIJ44_02525"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
                     /protein_id="HEY5164807.1"
                     /translation="FGAAWTGVPYAPLNYRLPADARQALLARLAPVEVADDSWLEAPT
                     TPSAPYPDDPAAPAVLLFTSGTTAEPKAATLTHDQLLAYLFNTVEFGSAGDDEAVLVS
                     VPPFHIAGVAAILSSTYAGRRVVPLPRFSPEAWLAVTAREGVTHAFVVPTMLARVVAQ
                     LEQDPDLKVSSLRHLAYGGSRMPTPILERALTLFPNAEFVNAYGLTETSSTVCILGPD
                     DHRAAQHSADATVRARLGSVGRPVPGIEVRVVDSEIQVRGAQVSGDYVGQGSQRDADG
                     WLHTGDRGRVDDDGFVFVDGRGDDTIIRGGENLAPAEIEDTLLRHPAVAGAAVVGIPD
                     EEWGEQVAAMVVARPGAALDPDALRGFVRDHLGSLKTPQVLVIVDELPHTATGKILRR
                     EVRAGLSKRR"
     gene            complement(1412..1921)
                     /locus_tag="VIJ44_02530"
     CDS             complement(1412..1921)
                     /locus_tag="VIJ44_02530"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY5164808.1"
                     /translation="MSTARHPIPGDVLDLQPEATAAFWRLYGTLWSHGTVDQATKEVA
                     RLRNARTTGCRYCQNVRFDGARQAGLTEDRVALIADEFETSDLSPAEKTVIRFVDSFL
                     ADPHGLTDEQRHEVLEHFDAGQVVELTAGVALFMGFSKIAVALGPPPDMPTLVVPTPD
                     WPPITDGQP"
     gene            complement(1948..2469)
                     /locus_tag="VIJ44_02535"
     CDS             complement(1948..2469)
                     /locus_tag="VIJ44_02535"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY5164809.1"
                     /translation="MSWLPEQVEGASPLDRVFGLRPDLYEPFRAFYATFWTHDLLDPI
                     VLELCRLRVAQLLRCESEQWVRYEPALRAGLTEEQVARLAQWPTTPMFSPAQRAALGF
                     AEQFVIDVEGIDAQLRDAVVDHFTVAGLVALCEALALFDGFCRFRTMLGVDGDSRSEV
                     IVVPGPTPREGLR"
     gene            complement(2477..3256)
                     /locus_tag="VIJ44_02540"
     CDS             complement(2477..3256)
                     /locus_tag="VIJ44_02540"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5164810.1"
                     /translation="MNADTGPPLTGRTAIVTGAGQGVGRGIARALAARGANVVLAARR
                     AETGEPSAQEIRDRGGEAACLVTDVTRAESVERCVADTVERYGGLHIMVHNAFRGGRP
                     HRLEDVDDALWTMNSRTAVWGSFYAARFAYPHLRTAESRGRLILITSPSGVEGSASIP
                     IYSPVKAAQRAMAKSLSREWGPFGITVNCLAPVAETPALTNAFTENPALKERLEARTS
                     LRRVGDAERDIGAVAVFLASDDAGYVTGQTIVCDGGSFMGL"
     gene            complement(3253..3999)
                     /locus_tag="VIJ44_02545"
     CDS             complement(3253..3999)
                     /locus_tag="VIJ44_02545"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5164811.1"
                     /translation="MSALAGRVAVITGADQPLGRGLALAVANEGANVALLGDEGLLTA
                     VAQEASEHGARAVAFPALLTERHDVERAFKQAAETFGAPVSAVVHTAMTPIAYEPIAF
                     ADIDDERWGLIWEGTMRAALAVLQASFAPMRGHDGRIVLVAPTVSMSGAERLVSYTTA
                     VEGQRLLAKSAARQWGPDGITVNCVAVAPEQVPIGVSSTATSLAPAALGGIGDSEHDI
                     GPVVVFLLGDAGRFVTGATISVDGGVWMAP"
BASE COUNT          621 a         1484 c         1378 g          634 t
ORIGIN      
        1 ttcggggccg cgtggacggg agtgccgtac gctccgctca actaccggct ccccgcggat
       61 gcccgtcagg cgctgctcgc ccggctggcg ccggtcgaag tcgccgatga ctcctggctc
      121 gaggctccga ccaccccgag cgccccgtac cccgatgacc cggccgcgcc ggcggtgctg
      181 ctcttcacca gcggtaccac cgcggaaccg aaggccgcga ccctcaccca cgaccagctg
      241 ttggcgtatc tcttcaacac cgtcgagttc ggctccgccg gcgacgacga ggcggtgctc
      301 gtctcggtcc ctccgtttca catcgcgggg gtcgccgcga tcctgtcctc cacctacgcc
      361 ggacggcggg tcgtccccct cccccggttc tcgccggagg cctggctggc ggtgacggcc
      421 cgggagggcg tgacccacgc gttcgtggtc cccaccatgc tggcccgcgt cgttgcccag
      481 ctcgaacagg acccggacct caaggtgtcg tcgcttcgcc atctcgccta cgggggctcg
      541 cggatgccga ccccgatcct ggaacgagca ctcacgttgt ttccaaacgc ggagttcgtg
      601 aacgcgtacg gcctcaccga gacgagcagc accgtctgca tcctgggacc cgacgaccat
      661 cgcgccgcgc agcacagcgc cgacgcgacg gttcgagccc ggctcgggtc tgtcggccgc
      721 ccggtcccgg gcatcgaggt ccgagtcgtc gacagcgaga tccaggtccg gggcgcgcag
      781 gtctccggcg actatgtcgg acagggatct caacgcgacg ccgacggctg gctccacacc
      841 ggtgaccggg ggcgggtcga tgatgacggg ttcgtcttcg tcgacggacg gggtgacgac
      901 acgatcatcc gcggcggcga gaacctcgct cccgccgaga tcgaggacac actgctgcgc
      961 caccccgcgg tcgccggcgc ggcggtcgtc ggcattcccg acgaggaatg gggtgaacag
     1021 gtcgcggcca tggtcgtcgc ccgacccggc gccgcgctcg accccgatgc cctgcgtggc
     1081 ttcgtccgtg accacctcgg gtccttgaag accccgcagg tgctcgtcat cgtcgacgag
     1141 ctaccccaca ccgccaccgg caagatcctg cgtcgcgagg tgcgagccgg tctctccaaa
     1201 cgtcgatgaa cgcccgagac atctcgcgcc cgacgttcct cggcgcaacc atgaccgctg
     1261 ccgcctcgcc tcggtggatc accagctgag cctcgtcccg gaccggtgcg cccattctcc
     1321 tcggggtgaa cgccgcgacg tacgcccgcg acctcgagac tcgaacccaa ccccaggtcg
     1381 tcgctgacgc gatggacgtg ctgggatcga tctacggctg accatcggtg atcggtggcc
     1441 agtccggtgt gggaacgacc aaggtcggca tgtcgggcgg cggcccgagc gcgaccgcga
     1501 tcttcgagaa ccccatgaag agcgccacac cggcggtcag ctcgacgacc tgaccggcgt
     1561 cgaagtgctc gagcacttcg tggcgctgct cgtcggtcag gccgtgcggg tcagcgagga
     1621 acgagtccac gaaacggatc accgtcttct cggccggtga gagatccgac gtctcgaact
     1681 catccgcgat cagcgccacc cggtcctcgg tgaggccagc ctggcgagcc ccgtcgaagc
     1741 ggacgttctg gcagtagcga cagcccgtcg tcctcgcgtt gcggagccgg gcgacctctt
     1801 tcgtagcctg gtcgaccgtc ccgtggctcc acagcgtgcc gtacagccgc cagaacgcgg
     1861 ccgtcgcttc gggctgcaag tcgagcacat ctcccgggat cgggtgtcgc gccgtgctca
     1921 tctcagtgcc gctcagacat cgtcgggtca gcgtaggcct tcgcggggcg tcgggccggg
     1981 gaccacgatc acctctgacc tggaatcgcc gtcgacaccg agcatggtgc ggaatcgaca
     2041 gaagccgtcg aagagggcga gggcctcaca gagtgccacc agacccgcga cggtgaaatg
     2101 gtcgacgacc gcgtcgcgca gctgggcgtc gattccctcc acgtcgatca cgaactgctc
     2161 ggcgaagccg agcgcggccc gttgagcggg gctgaacatc ggtgtcgtcg gccactgcgc
     2221 gagccgcgcc acctgctcct cggtcaggcc ggcccggagt gcgggctcgt agcgcaccca
     2281 ctgctcgctc tcgcaccgca gcagctgcgc gacccggagg cgacacagtt cgagcacgat
     2341 cgggtccagg aggtcgtggg tccagaacgt cgcgtagaag gctcggaacg gctcgtataa
     2401 gtccggtcgc agcccgaaga cccggtcaag cgggctcgct ccttctacct gttcaggtaa
     2461 ccagctcatc cgccgctcac aagcccatga agctgccacc gtcacagacg atggtctggc
     2521 ccgtcacgta ccccgcgtcg tcgctcgcca ggaacacggc gacagcgcca atgtcgcgct
     2581 cggcgtcccc gacgcgtcga agtgacgtgc gcgcctcgag gcgttccttc aatgcgggat
     2641 tctccgtgaa cgcgttcgtg agcgccgggg tctccgcgac cggcgcgagg cagttgacgg
     2701 tgatcccgaa tgggccccac tcgcgagaca ggctcttggc catcgcccgc tgcgcggcct
     2761 tcactggcga gtagatcggg atgctcgcgc ttccttcgac tcccgacggc gaggtgatca
     2821 ggatcaggcg gccgcgagac tcagcggtac gcaggtgcgg ataggcgaat cgggccgcgt
     2881 agaacgatcc ccagaccgcg gtgcgtgagt tcatggtcca gagcgcgtcg tcgacgtctt
     2941 cgagtcgatg cggtcgaccg cctcgaaacg cgttgtggac catgatgtgg aggccgccat
     3001 agcgctcaac cgtgtccgcg acgcagcgtt cgacactttc ggcgcgggtg acgtccgtga
     3061 ccaggcaggc ggcttcgcca ccccggtccc gaatctcttg ggcggatggt tcaccggtct
     3121 cggcccggcg ggcggccagc acgacgttcg cgcctcgggc cgcgagggct cgggcgatcc
     3181 ctcgccctac cccctggccg gcgccggtca cgatcgcggt ccgtcccgtc aacggcggcc
     3241 cggtgtcggc gttcacgggg ccatccacac gccgccgtcg acactgatcg tggcgccggt
     3301 cacgaaacga ccagcgtcgc cgaggaggaa cacgacgacc ggtccgatgt cgtgctcgga
     3361 gtcaccgatg ccgccgagcg ccgccggcgc gagcgacgtc gcggtgctcg acaccccgat
     3421 cgggacctgc tccggtgcca ccgcgacgca gttcacggtg atcccgtcgg gaccccattg
     3481 acgggccgcg gacttggcca gcaggcgttg gccttccacc gcggtcgtat aggagaccag
     3541 ccgttcggcc ccagacatcg agaccgtcgg ggcaaccaag acgatccggc cgtcgtggcc
     3601 gcgcatcgga gcgaacgacg cctgcagcac cgccagcgcg gcgcgcatcg ttccttccca
     3661 gatgaggccc caacgctcgt cgtcgatgtc ggcgaacgca atgggctcgt acgcgatcgg
     3721 ggtcatcgcg gtgtgcacca ccgcgctgac cggcgctccg aacgtctcgg ccgcctgctt
     3781 gaaggctcgc tcgacgtcgt ggcgctcggt gagtaacgcc gggaaggcga cggctcgagc
     3841 gccgtgctcg ctcgcctctt gcgccaccgc cgtgagcaga ccctcgtcgc cgaggagcgc
     3901 gacgttcgcc ccttcgttgg ccaccgcgag cgccagtccg cggccgaggg gctggtccgc
     3961 gccggtgatg accgcgaccc tgcccgcgag cgcgctcatc gcccggaccg tatcatccgg
     4021 ccgtggaaac tgatcaaggc gtgcgcgcgc tcgtggcgcg ctcctgtgac gccgtcgcgc
     4081 gcgcggatgt ggaagcgtgg atcgcgtgtt ggcgtga
//
LOCUS       DATCCH010000128         2554 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_470535, whole genome shotgun sequence.
ACCESSION   DATCCH010000128 DATCCH010000000
VERSION     DATCCH010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470535"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VIJ44_02550"
     CDS             complement(<1..219)
                     /locus_tag="VIJ44_02550"
                     /inference="COORDINATES: protein motif:HMM:NF015666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEY5164812.1"
                     /translation="MGFSRDAVIVGGCGRVGLPLGLALADSGLAVTLYDRDAFAVDRV
                     RAAKMPFFEREADAVLERLISTDKLYVTT"
     gene            complement(332..1024)
                     /locus_tag="VIJ44_02555"
     CDS             complement(332..1024)
                     /locus_tag="VIJ44_02555"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY5164813.1"
                     /translation="MPDDHDDAPNAPADIGGLDYGRLYEFRFRDVEQRARQTVWNEIG
                     PFVWKRMGSPARVLDPAGGRGEFVNSIAADERWLVDLVDYPHRQTVPGVKVVIGDVLT
                     LDLPRDYFDGIFVSNLLEHLPDPDAVAGFLARMREALAPGGVLAIMGPNFKYCAREYF
                     DCADHLLALTHVSVEEHLIAAGFEVHENVARFLPFSFRSRLPSSARLTRLYLQMPLLW
                     RVAGSQFLLLAR"
     gene            complement(1080..1718)
                     /locus_tag="VIJ44_02560"
     CDS             complement(1080..1718)
                     /locus_tag="VIJ44_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164814.1"
                     /translation="MAKSTSGTGWRQRSSGFLGGIAAVVLVVVLLGGMALGYEIEKGR
                     VKKPAKPAAAAAKKAPAKAVPVRVVGTVSAKTATSITVTPTKGAARKITIAKGTVVVK
                     AGSGAASDIAANARVVFSGTFTAAKAVIVLPTTARIGTKVTAADGTSMSLQNGKKVTK
                     ITTTGASVNKTSPATVADVVNGAKVLVGAVRTKAGAVIATEIVVLPGNSAFQ"
     gene            1864..>2554
                     /locus_tag="VIJ44_02565"
     CDS             1864..>2554
                     /locus_tag="VIJ44_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164815.1"
                     /translation="MTNPGRGMRLGFVEPHLGRFGGIRRMVEFANRLVDRNHQVTFYL
                     PDDVDLRCTWMRCAAEIKPITEGFDDELDVILFNHEPQWHLIDRFERARRRVFYALHY
                     AHLYGKEGSWESLRAPVDLQLANSNWTADQIGAETGHRPTVQLGGVNREMFHPYPGPK
                     RFSLLCSGEQQREWKGTETIVEAGRLLRVRVERYAGKNLDQEALGREYAAARVFAVGS
                     WFEGFCQPGLEA"
BASE COUNT          417 a          840 c          869 g          428 t
ORIGIN      
        1 cgtagtcacg tagagtttgt cggtcgagat cagccgttcg aggaccgcgt cggcctcgcg
       61 ctcgaagaac ggcatcttgg ccgcccgcac ccggtcgacc gcgaacgcgt cacggtcgta
      121 gagggtgacc gccaagccgg aatcggcgag ggcgaggccc aagggaagcc cgacccggcc
      181 gcaaccgccg acgatcactg cgtctcgaga aaatcccatg ccctgctgtc cccttgtcca
      241 gtggccccac ccgaccaggg tgtttgacca cagacggtag caaggacgcc cccgagttgg
      301 aagggttcgg gactgtacag gggcagtctc ttcagcgcgc cagcaacagg aactgacttc
      361 cggcgactcg ccagagcagc ggcatctgca gatacaggcg ggtgaggcgg gccgaggagg
      421 gcaagcggct ccggaacgaa aacggcaaga accgcgcaac gttctcgtgc acctcgaacc
      481 ccgctgcgat caggtgctcc tcgaccgaga cgtgggtcag ggcgagcagg tggtcggcgc
      541 aatcgaagta ctcgcgcgcg cagtacttga agttgggccc catgatcgcg agcacgccgc
      601 ccggggccag cgcctcgcgc atgcgggcga ggaagccggc gaccgcgtcg ggatcgggga
      661 ggtgctccag gaggttcgag acgaagatcc cgtcgaagta gtcgcggggg aggtcgagcg
      721 tgagcacgtc gccgatcacg accttgacgc cgggcaccgt ctgtcggtgc gggtagtcga
      781 cgaggtcgac gagccagcgt tcgtcggccg cgatcgagtt cacgaactcg ccgcgccctc
      841 ccgcgggatc gagcacccga gcgggggacc ccatgcgctt ccacacgaag ggcccgatct
      901 cgttccacac ggtctgacga gcccgctgct cgacgtcgcg aaagcggaac tcgtagaggc
      961 gcccgtaatc gagcccgccg atgtcggccg gagcattcgg cgcgtcatcg tgatcgtcag
     1021 gcacgcgggg gcagtttcgt cacgccattg gtaattctct tggactattg gctcttggac
     1081 tattggaagg cgctgttgcc cgggagcacg acgatctccg tcgcgatcac cgccccggcc
     1141 ttggtgcgaa cggccccgac caacaccttg gccccgttga ccacgtcggc gacggtcgcg
     1201 ggcgaggtct tgttgacgct cgcgccggtg gtcgtgatct tcgtcacctt cttgccgttc
     1261 tgcaagctca tcgacgtccc gtcggccgcg gtgaccttcg tgccgatgcg cgcggtggtc
     1321 gggaggacga tgacggcctt ggccgccgtg aacgttccgc tgaagacgac tctcgcgttg
     1381 gcggcgatgt cactcgccgc gcccgatccg gccttcacga cgacggttcc cttggcgatc
     1441 gttatcttgc gagccgcgcc tttcgtgggc gtgacggtga tcgaagttgc cgtcttcgcg
     1501 ctgacggtgc ccacgacgcg gaccgggacg gccttggccg gagctttctt cgcggcggcg
     1561 gcggcgggct tcgccggttt cttgacccgg cccttttcga tctcgtagcc gagcgccatc
     1621 cctcccagga gcacgacgac gagcacgacc gcggcgatgc ccccgagaaa gccggacgac
     1681 cgctgtcgcc atccggtgcc gctcgttgat ttcgccattc cgtgctcctc gcgaccgctg
     1741 agtgatgtga tgaagggtca tgttgtagca aacctgcgag ggaggttcca cgcgcacggt
     1801 ccctgtggac aacgctttgt cacggtttcg ggccctccat cggcccgagt agcgtgatcg
     1861 gccatgacga atcctgggcg gggcatgcgc ctcggtttcg ttgagccgca cctcggccga
     1921 ttcgggggga ttcggcgaat ggtcgagttc gccaaccggc tcgtggatcg caaccaccag
     1981 gtgacgttct atctccccga cgacgtggat ctgcgctgca cctggatgcg gtgcgccgcg
     2041 gagatcaagc cgatcaccga aggtttcgac gacgagctcg acgtgatcct gttcaaccac
     2101 gaaccgcagt ggcacctgat cgatcggttc gagcgggccc ggcgacgggt gttctacgcc
     2161 ctccactacg cccacctcta cggcaaggag ggcagctggg agagtcttcg ggcccccgtc
     2221 gatctccagc tcgccaacag caactggacc gccgaccaga tcggggccga gaccgggcac
     2281 cggccgacgg tgcagctcgg cggcgtcaat cgggagatgt ttcatcccta ccccgggccg
     2341 aagcgattct ccctgctctg cagcggcgag cagcagcgcg agtggaaggg caccgagacc
     2401 atcgtcgagg ccggccgcct gctccgcgtg cgggtcgagc ggtacgcggg caagaacctc
     2461 gatcaagagg cgctcgggcg ggagtacgcg gcggcccggg tcttcgcggt cggcagctgg
     2521 ttcgagggct tctgccagcc cgggctcgag gcgc
//
LOCUS       DATCCH010000129         2061 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_97068, whole genome shotgun sequence.
ACCESSION   DATCCH010000129 DATCCH010000000
VERSION     DATCCH010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97068"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            353..1309
                     /locus_tag="VIJ44_02570"
     CDS             353..1309
                     /locus_tag="VIJ44_02570"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019873173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEY5164816.1"
                     /translation="MAERRDLVAQIGANKRRTVYLMAGFAVLITGVVVAFDVAFRFGP
                     AFIVIAFVVALVLVWASYFYSDKLAISAARAHEADPQEYRQLHDVVEELCIGVGLPKP
                     RVYIIDDPAPNAFATGRNPQHSAVAVTAGLLQIMNRTELEGVLAHELSHIRNYDILIS
                     TIAVTLVGFVALLADFGLRMLFFGGLGGRGRDNEGGGQIAIFILVISVVFIIIAPIAA
                     KIMQFAVSRRREALADVSGIYITRNPQGLISALEKLRDTSGVVHHAPAATAHMWITSP
                     LDKKSKDAHGWFNRLFETHPPIEQRIAALQEVAGSVQPPATA"
     gene            complement(1368..1619)
                     /locus_tag="VIJ44_02575"
     CDS             complement(1368..1619)
                     /locus_tag="VIJ44_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164817.1"
                     /translation="MKRLADYGDDDHPADDPERVKVAWAVAALDDCDGCDALRIELTV
                     EEMDRPGTGLVAHLAPGTARRLRAALATALRDIGEDSGS"
BASE COUNT          304 a          740 c          677 g          340 t
ORIGIN      
        1 tctcctccag agcgccgtcc ccgccggcga cgacgggacg cgtgcccgcc attgatgtcg
       61 cctcgcacgg tgggcggggc gcaggggtga cacctccgag gccgctgagc acgacatcgc
      121 gtcgagtgct ggcccgctgc cctcggtgcc cccacgcgtg tcggtagccg gttcgggttc
      181 ggtgtcgcag gtgcgaagca gcgccggagc ctcgagtcag cccagagcgg cccgggtcgg
      241 atcggagccc gccgggccgg ggtcgctcgg cggcaccgtc ccgacccgtt ggccgccgac
      301 cctgcgttcg ggcaatgctg acgtccaaca cgctcgaacg gagggaccga agatggctga
      361 gcgcagggat ctggtcgctc agatcggggc caacaagagg cggacggtgt atctgatggc
      421 ggggttcgcc gttctcatca ccggcgtcgt cgtggccttc gacgtggcgt tccggttcgg
      481 gccggcgttc atcgtcatcg ccttcgtggt tgcgctcgtg ctggtgtggg ccagctactt
      541 ctattcggac aagctcgcca tctccgccgc tcgtgcccac gaggccgacc ctcaggagta
      601 ccgccagctg cacgacgtcg tggaggagct ctgcatcggg gtcggcctcc ccaagccgcg
      661 cgtctacatc atcgacgacc cggccccgaa cgccttcgcc accggtcgca acccgcagca
      721 ctctgccgtc gcggtgaccg ccggcctgtt acagatcatg aaccggaccg aactcgaagg
      781 cgtcctcgcc cacgagctca gccacatccg caactacgac atcctcatct cgacgatcgc
      841 ggtcaccctc gtcgggttcg ttgccctgct cgccgacttc gggctgcgga tgctcttctt
      901 cggaggcctc gggggacgcg gacgcgacaa cgagggtggt ggccagatcg ccatcttcat
      961 cctcgtgatc tcagtcgtct tcatcatcat cgccccgatc gccgccaaga tcatgcagtt
     1021 cgcggtttcg cgacgtcgcg aggcactcgc ggacgtgtcc ggcatctaca tcactcgcaa
     1081 cccgcaaggg ctcatctcgg cgctcgaaaa gctccgcgac acctcagggg tcgtgcacca
     1141 tgccccggcc gcgaccgcgc acatgtggat cacgtcaccg ctggacaaga agtcgaaaga
     1201 cgcccacggc tggttcaacc ggctcttcga gacccaccca ccgatcgagc agcgcatcgc
     1261 cgcgttgcaa gaagtcgctg gcagcgtcca gccaccagcg acagcctgaa cggccgtcca
     1321 ctgtcgccca gcaccagcct gcacgaccag gtgctagccg ggaggcgtta ggagccggag
     1381 tcctcgccga tgtcgcgcag cgcggtcgcg agcgcggctc ggaggcgacg agccgtgccc
     1441 ggtgcgagat gcgcgacgag accggtgccg ggacggtcca tctcctcgac ggtcagctcg
     1501 atgcgcagcg cgtcgcagcc gtcgcagtca tccagcgcgg cgaccgccca cgcgaccttg
     1561 acccgttcgg ggtcatccgc cgggtggtcg tcgtcgccgt agtccgcgag tcgcttcatc
     1621 cggccagtta acccgatccg gcgaccgacc gaccccgacg cccggtcggg atttctcggg
     1681 ccgttgagcg accgcgtcgg ccccgttccg tagggcgatg gacccggcct ctggcacccg
     1741 ttggccgccc caccgcccgc cggtcagccc agcgggatac ggcccgtgga agtgagctcc
     1801 ttccgtgaga agggcttgag ttgcccggtt tcggatccga tgaaccggtg aggcggctac
     1861 gagcggccgt cgacgtcgcg cgtcaggact cggagcacac cgcatgcaag gcatctggat
     1921 ctggcgacgc tggactcgct ggcatctgac gcctgggatc ctggtcgtcg ccgcggtggt
     1981 cctcggcctc gacgtcgtca cggcttggcg caacgtcgcg atcggctccc tgggtcgcgt
     2041 tgcgacgtcg ccggcgcttc c
//
LOCUS       DATCCH010000130         5030 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_421832, whole genome shotgun sequence.
ACCESSION   DATCCH010000130 DATCCH010000000
VERSION     DATCCH010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5030
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421832"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..638)
                     /gene="pth"
                     /locus_tag="VIJ44_02580"
     CDS             complement(21..638)
                     /gene="pth"
                     /locus_tag="VIJ44_02580"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEY5164818.1"
                     /translation="MLRRRGSPPARTGTPADLLVVGLGNPGAEYAHTRHNVGVDVVEI
                     LADRHGAKLRSGKEHALVDEVRIGARRVALAVPQQWMNDSGDAVRRLVRRYGVTPEQL
                     VVVHDELDLPVATLRVKSGGGLAGHNGLRSVQAHLHSDAFGRVRIGVDKPGSKDRGAD
                     HVLSRFSKRERRDIDVALQQAADAVEVIVGEGIDAAMNRFNPSPA"
     gene            complement(642..1310)
                     /locus_tag="VIJ44_02585"
     CDS             complement(642..1310)
                     /locus_tag="VIJ44_02585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HEY5164819.1"
                     /translation="MAEEVLTAEPRAERGSRPAGRLRRAGQVPAVIYGLGGDTVPVTV
                     PSREVTHILSHGANTLIRLRLAGDDQLTLARQVQRHPTRGDLVHLDFIRVRTDVAVSA
                     EVALHLIGEPEGVRNGGLLEQLVFGLPIEAMPHDIPGSIEVEVGHLDIGDQFRLNEIT
                     LPAGVTATIEPDALIAQVVAPRVAEVAEVAEGEGEEGEAVEGEAGEGAPAGEAPSGDE
                     GGGE"
     gene            complement(1532..2524)
                     /locus_tag="VIJ44_02590"
     CDS             complement(1532..2524)
                     /locus_tag="VIJ44_02590"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0044249 - cellular biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEY5164820.1"
                     /translation="MTHLEFTPTKRLMLFAGRGNGDVSAEIAQGLRVPLGSVVLSSFA
                     NGELYCRYGENIRGADVFVIQSHCEPINDRIMEQLIMIDAAKRASAKRITAVCPFYAY
                     ARQDRKAEGREPITARLLADLLTAAGADRVVTVDLHTGQIQGFFDMPVDHLTAVPLLT
                     AHLAAVVAPDVTVVSPDAGGGKLARRFANNLADHKIDAELAFIDKRRPKGLHNLAVAV
                     EVVGDVEGRSCVLVDDMIDTAGTISSAADLLHTRGASEVIVAATHGVLSGPAVDRLKN
                     APIREVVVTNTLPIPDEKRWDALTVLSIAPIIAKAINAVFEDSSVSEIFRGDNF"
     gene            complement(2521..3951)
                     /gene="glmU"
                     /locus_tag="VIJ44_02595"
     CDS             complement(2521..3951)
                     /gene="glmU"
                     /locus_tag="VIJ44_02595"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEY5164821.1"
                     /translation="MTELAYRPLAAVVLAAGEGTRMRSATPKVLHPLCGRPMILHVLD
                     RLVELPLARIVIVVGHGAESVTKTVQEQLATEIPVEFVEQRFQRGTGDAASVGLTGSG
                     FDFDGEDDVLVLTGDTPLLRADTLAQLATEHRLTDAAATVLTAELDDPTGYGRIVRDA
                     RGGVNRIVEQPDANDAEREITEVNSSIYCFRRSLLAPALRRLDPENAQGEYYLTDVIG
                     VLRATGHVVVGTPADDATETLGVNDRAQLAAAEAVLRGRINRYWMREGVAMTDPDTTY
                     IDASVDLEAEVRLLPGTILEGRTAVGARTVIGPHCRLVDTIVGEGAVIEQTVAREAEI
                     GDGATIGPWVSLRPGTRIAAGAHVGTFVETKNADIGEGAKIPHLAYVGDAEIGPGANI
                     GAGTITANYDGRDKHRTKVGRDARIGSNTVLVAPVEVGDEAYTAAGAVVTDDVPPGAL
                     AKGVPARVEEGWVEARRHADEDEDGQ"
     gene            complement(3990..4060)
                     /locus_tag="VIJ44_02600"
     tRNA            complement(3990..4060)
                     /locus_tag="VIJ44_02600"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4026..4028),aa:Gln,seq:ttg)
     gene            4158..4259
                     /locus_tag="VIJ44_02605"
     CDS             4158..4259
                     /locus_tag="VIJ44_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003402602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AURKAIP1/COX24 domain-containing protein"
                     /protein_id="HEY5164822.1"
                     /translation="MGSLIKKRRKRMRKKKHKKLLKKTRWQRRQQGK"
     gene            complement(4303..>5030)
                     /locus_tag="VIJ44_02610"
     CDS             complement(4303..>5030)
                     /locus_tag="VIJ44_02610"
                     /inference="COORDINATES: protein motif:HMM:NF012509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEY5164823.1"
                     /translation="TFTAGGDTAHVPNGAANLAVRAAEDLLIRAGRSGHGVRMWLRKA
                     IPAGAGLGGGSADAAAALAAVRRMLEVDISDEELRELAATIGSDVPFCLEGGAAWMRG
                     RGERLEPVSLDGQLPLVVALPPFRISTPDVYRAWDELGGPIAERRIAAPPAAAGIITG
                     LANDLEVAAERVEPRLVSFREELEAVAKAPVILAGSGSAYAVIPDVDSLGAAGALANR
                     VSSKLRVPATATSIAAQGVRLSA"
BASE COUNT          684 a         1645 c         1942 g          759 t
ORIGIN      
        1 ggcgagcttg aagaagggat tcatgccggc gaggggttga agcggttcat ggcggcgtcg
       61 atgccctccc cgacgatgac ctcgacggcg tcggccgcct gttggagcgc gacgtcgatg
      121 tcgcgccgct cacgcttgga gaagcgggac agcacatggt cggcgccccg gtccttcgag
      181 cccggcttgt cgaccccgat gcggacccgt ccgaaggcgt cgctgtgcag gtgcgcctgg
      241 accgaccgga gcccgttgtg gccggcgagg ccgccacccg acttcacgcg cagggtcgcg
      301 acggggaggt cgagctcgtc gtggacaacg acgagctgct ccggggtcac cccgtagcgg
      361 cggacgaggc ggcgcaccgc gtcgccggag tcgttcatcc actgctgggg gacggcgagc
      421 gcgacgcgcc gggcgccgat gcggacctcg tcgacgaggg cgtgttcctt gcccgaccgc
      481 agcttggccc cgtgccggtc ggcgaggatc tcgacgacgt cgacgccgac gttgtggcga
      541 gtgtgggcgt actcggcgcc ggggttgccg agaccgacca cgagcaggtc ggcgggcgtc
      601 ccggtgcgcg ccggcggtga gccccgccgc cgcagcacga gctactcccc gccgccctcg
      661 tcgccgctcg gagcttcgcc ggcgggtgcc ccttcgccgg cttcgccctc gacggcttcg
      721 ccctcttcgc cttcgccctc ggcgacttcg gccacctcgg cgacccgggg ggcgacgacc
      781 tgtgcgatga gcgcgtcggg ttcgatggtg gcggtcaccc cggcgggcag cgtgatctcg
      841 tttaagcgga actggtcgcc gatgtcgagg tggccgacct cgacctcgat cgagcccggg
      901 atgtcatggg gcatggcctc gatcgggagg ccgaacacga gctgctcgag cagtccgccg
      961 ttgcggacgc cttcgggctc accgatgagg tggagcgcca cctcggcgct gacggcgacg
     1021 tcggtgcgga cccggatgaa gtcgaggtgg accaggtcgc cgcgggtggg gtggcgttgc
     1081 acctggcggg ccaaggtgag ctggtcgtcg ccggcgaggc ggagccggat caaggtgttg
     1141 gcgccgtggg agaggatgtg ggtcacctct cgggacggga cggtgacggg gacggtgtcg
     1201 ccgcccaggc cgtagatcac ggcgggcacc tggccggcgc gccgaagccg ccccgcgggg
     1261 cgcgagcctc gttcggctcg gggttcggcg gtgaggactt cctcggccat gcctggatgc
     1321 gcctttcaag atgggggacc gcccattctg ctcgcggggc ggcggtcagg ccaagccgag
     1381 ccgtggggtc gcctgggggc gccagggcga tgggtcaggg tcgccagcgc cgatcggggt
     1441 ggcgcaccgg cgcgtcgagc tggcggggga tcgtgaacgg gagacggcgc tcgcgctatg
     1501 ggcgatgaag ttgcgcgcga gtgatgtggt gtcagaagtt gtcgccgcgg aagatctcgc
     1561 tgacgctcga gtcctcgaag acggcattga tggccttggc gatgatcggc gcgatcgaga
     1621 gcacggtgag ggcgtcccag cgtttctcgt cggggatcgg caaggtgttg gtgacgacca
     1681 cctcgcggat cggcgcgttc ttgagtcggt cgaccgccgg gcccgacagg acgccgtggg
     1741 tggcggcgac gatgacctcg gaggcgcccc gggtgtggag caggtcggcg gcggagctga
     1801 tagtcccggc ggtgtcgatc atgtcgtcga cgaggacaca ggaccggccc tcgacgtcac
     1861 cgaccacttc gaccgcgaca gccaggttgt gcaggccctt gggtcggcgc ttgtcgataa
     1921 aggccagctc ggcgtcgatc ttgtggtcag cgaggttgtt cgcgaaacgc cgagcgagct
     1981 tgccgccgcc ggcgtcgggt gagaccaccg tgacgtcggg cgcgacgacc gcggccaggt
     2041 gggcggtgag gagcggcacc gcggtgaggt ggtcgacggg catgtcgaag aacccttgga
     2101 tctgcccggt gtgcaggtcg acggtcacga cccggtcggc gccggccgcg gtgagcaggt
     2161 cggcgaggag ccgggcggtg atcggctcgc gcccttcggc ttttcggtcc tggcgggcgt
     2221 acgcgtagaa cgggcagacc gcggtgatcc gcttcgccga cgcgcgtttc gcggcgtcga
     2281 tcattatgag ctgctccatg atgcggtcgt tgatcggctc gcagtggctc tggatgacga
     2341 acacgtcggc gccgcggatg ttctccccgt accggcagta gagctccccg ttggcgaagc
     2401 tcgacagcac caccgacccg agcgggaccc gcaggccctg ggcgatctcg gccgagacgt
     2461 cgccgttgcc gcgaccggcg aagagcatga gccgcttcgt cggggtgaac tcgaggtgcg
     2521 tcactgaccg tcctcgtcct cgtcggcgtg acggcgggcc tcgacccagc cttcctcgac
     2581 gcgggccggg acgcccttgg ccagcgcgcc gggcgggacg tcgtcggtga cgacggctcc
     2641 ggcggcggtg tacgcctcgt cgccgacctc gaccggggcg acgagcacgg tgttcgatcc
     2701 gatgcgggcg tcgcggccca ccttggtgcg gtgcttgtcc cggccgtcgt agttggccgt
     2761 gatggtcccg gcgccgatgt tggcaccggg tccgatctcg gcgtcgccga cgtaggcgag
     2821 atgcgggatc ttggcgcctt cgccgatgtc ggcgttcttg gtctcgacaa acgtgccgac
     2881 gtgcgcgccg gcggcgatgc gagtccccgg ccgcaacgac acccacgggc cgatggtggc
     2941 gccgtcaccg atctcagcct cgcgggcgac ggtctgctcg atgacggccc cttcaccgac
     3001 gatggtgtcg acgaggcggc agtggggtcc gatgaccgtg cgggccccga ccgcggtgcg
     3061 accctcgagg atggtgccgg gcaggagtcg cacctcggct tcgaggtcga ccgacgcgtc
     3121 gatgtaggtg gtgtcggggt cggtcatggc gacgccctca cgcatccagt agcggttgat
     3181 gcggccgcgg agcaccgctt cggccgcggc gagctgggcg cggtcgttga cgccgagggt
     3241 ctcggtggcg tcgtcggcgg gcgtgccgac gacgacatga ccggtggcgc gcaagacccc
     3301 gatgacgtcg gtcaggtagt actcgccttg cgcgttctcg gggtcgaggc gccgaagggc
     3361 gggggcgagg aggctgcggc ggaagcagta gatcgacgag ttgacctcgg tgatctcacg
     3421 ctcggcgtcg ttggcgtcgg gctgttccac gattcggttc acaccaccgc gggcgtcgcg
     3481 cacgattcgc ccgtagccgg tcgggtcgtc gagctcggcg gtgaggacgg tcgcggcggc
     3541 gtcggtgagg cgatgctcgg tggcgagctg cgcgagggtg tcggcacgga gcagcggggt
     3601 gtcgccggtc aggacgagga cgtcgtcttc gccgtcgaag tcgaacccgg acccggtgag
     3661 gccgacgctg gcggcgtcgc cggtgccgcg ctggaagcgc tgctcgacga actcgaccgg
     3721 gatctcggtg gcgagctgct cttgaacggt cttggtgacc gactccgcgc cgtgaccgac
     3781 gacgatcacg atgcgggcca acgggagctc cacgagccgg tcgaggacgt ggaggatcat
     3841 gggccgccca cacagggggt ggagcacctt cggggtcgcg gaccgcatgc gggtgccctc
     3901 cccggcggcg agcaccaccg cggcgagagg tcggtatgcc agctccgtca ccccttccat
     3961 ggcagccatt caatcgtgcg acggcgtcgc tcgggggcga ggtttcgaac ctcgacttgg
     4021 gactccaaag gaccccgtgc tgccgttaca ccacccccga caaggttctc gtcgaatgag
     4081 gctacccccg aggttgtggc gtaccgcgca cgccgatgtg acttcggagg tcgtcacggg
     4141 taccgtgcgc agcagtcatg ggatcgctga tcaagaagcg ccgcaagcgc atgcgcaaga
     4201 agaagcacaa gaagctgctg aagaagaccc gctggcagcg tcgccagcag ggcaagtagt
     4261 ccggcggccg ccccgacagg gcggccgttt cgcgtcgggg cctcaggcgg agaggcgcac
     4321 cccttgcgcg gcgatggacg tcgccgtggc cggcacccgc agcttcgagc tgacccggtt
     4381 ggcgagcgcc cccgcggcac cgaggctgtc gacgtcgggg atgaccgcgt acgccgagcc
     4441 gctcccggcg aggatcacgg gggccttcgc gacggcctcg agctcctcgc ggaacgacac
     4501 cagccgcggc tcgacccgtt cggcggcgac ttcgaggtcg ttggcgagcc cggtgatgat
     4561 gcccgccgcg gccggtgggg cggcgatgcg ccgctccgcg atggggccgc ccagctcgtc
     4621 ccacgcccgg tacacgtcgg gggtggagat ccgaaacggg ggcaaggcga cgacgagggg
     4681 gagctggccg tcgaggctca ccggctcgag ccgttcaccc cggccgcgca tccacgccgc
     4741 cccgccctcc aggcagaacg gaacgtcgga gccgatggtg gcagccaact cgcgcagctc
     4801 ctcgtcgctg atgtcgacct cgagcatgcg acggaccgcg gcgagcgccg cggccgcgtc
     4861 ggccgagcct ccgccgaggc ccgcgcccgc gggaatggct ttgcgcagcc acatgcgcac
     4921 cccgtggccg gagcgcccgg cgcggatcag gagatcttcg gcggcgcgca ccgcgaggtt
     4981 ggccgcgccg ttggggacgt gcgcggtgtc gccgccggcg gtgaacgtga
//
LOCUS       DATCCH010000131         2269 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_97838, whole genome shotgun sequence.
ACCESSION   DATCCH010000131 DATCCH010000000
VERSION     DATCCH010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97838"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1191
                     /gene="manB"
                     /locus_tag="VIJ44_02615"
     CDS             <1..1191
                     /gene="manB"
                     /locus_tag="VIJ44_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222900.1"
                     /note="converts mannose-6-phosphate to
                     mannose-1-phosphate; the resulting product is then
                     converted to GDP-mannose by ManC which is then used in the
                     synthesis of mannose-containing glycoconjugates that are
                     important for mediating entry into host cells; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEY5164824.1"
                     /translation="FADGATLAGADVVDLGLASTDLVYFASGYLDAPAAMFTASHNPA
                     QYNGIKLCRAAAAPVGEATGLTEIKARIAAGRIDTAETRGSVERRDLLSEFVTHVRSF
                     IDVETLRGQRLVTDTANGVGGLIVPAVFNGVPVELRMLFADLDGTFPNHPADPIQPEN
                     LKDLQQAVVESGADVGLAFDGDADRVFLVDDRGEPLSGSLTTAIVAASTLRRLPPDAT
                     DADRTIVHNLICSKAVPEIVRELGGTPVRTRVGHSYIKQVMAETGASFGGEHSGHYYF
                     RENYRADSGIIAALVTLETLAQAGVTLSELRHPYERYVQSGEINRRVDDPPAVIEAVA
                     AASVGATQDRLDGLTVDHGDWWFNLRSSNTEPLLRLNLEAADEASCRQHRAEILALLG
                     GEAD"
     gene            1197..1433
                     /locus_tag="VIJ44_02620"
     CDS             1197..1433
                     /locus_tag="VIJ44_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEY5164825.1"
                     /translation="MTLDPKLLDILACPEDKGPLLYFPDESSLYNPRLKRRYAVRDDI
                     PIMLIDEAETVSDTEHARLLAKADAEGIQPTFEA"
     gene            1439..>2269
                     /locus_tag="VIJ44_02625"
     CDS             1439..>2269
                     /locus_tag="VIJ44_02625"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEY5164826.1"
                     /translation="MPVDSLGLLGVVEGLPEQLAAAHEQAAMTLDRTALPHPDQIDNI
                     AVLGMGGSGIAGDVLRAVATSLPLPVIVLKQIRTPAFVGPRTLAFAVSYSGNTEETNA
                     MAEGALDAGARVIAVTAGGELARLAAARGAIQVQCAGGYVMPRAAVGALVAPLFVTLF
                     HIGLYPEAHALLVQAQAQLKHRCDQCRPDAGPGNPARELARRIARSIPLIYGGGALGG
                     VAAYRWKCDINENAKAPAFTAAYPELDHNEICGWGQHGDVTRQIMTLVSLRHGFEDER
                     L"
BASE COUNT          360 a          908 c          680 g          321 t
ORIGIN      
        1 ttcgccgacg gcgcgaccct cgccggcgcc gacgtcgtcg acctcggctt ggcgtccacc
       61 gacctcgtct acttcgcctc cggctacctg gacgccccgg cggcaatgtt caccgcgagc
      121 cacaatcccg cccagtacaa cggcatcaag ctctgtcggg ccgcggcggc cccggtcggt
      181 gaagccaccg gcctcaccga aatcaaagcg cgaatcgcgg ccgggcggat cgacaccgcc
      241 gagacgcgcg gcagcgtcga gcggcgcgac ctcctctcgg agttcgtcac ccatgtccgt
      301 tccttcatcg acgtcgaaac cctgcgcggg cagcgcctcg tcaccgacac cgccaacggt
      361 gtcggcggcc tcatcgtccc cgcagtgttc aacggggtgc ccgtcgagct ccgcatgctc
      421 ttcgccgacc tcgacggcac cttcccgaac caccccgccg accccatcca acccgagaac
      481 ctcaaagacc tccagcaggc cgtcgtcgaa tccggcgccg acgtcggcct cgccttcgac
      541 ggcgacgccg accgggtctt cctcgtcgac gaccggggcg aacccctctc cgggtccctc
      601 accaccgcca tcgtggcggc gtcgacgttg cgccgtctcc cacccgacgc caccgacgcc
      661 gaccggacga tcgtccacaa cctgatctgc tcgaaggcgg tgcccgagat cgtccgtgag
      721 ctcggcggca ccccggtgcg cacccgggtc gggcattcgt acatcaagca ggtgatggcc
      781 gagaccggcg ccagcttcgg cggcgagcac tccgggcact actacttccg ggagaactac
      841 cgggccgact cgggcatcat cgcggcgctc gtcaccctcg agaccctcgc ccaagccggc
      901 gtgacgctct ccgagctgcg ccacccctac gagcggtacg tccaatccgg ggagatcaac
      961 cgccgggtcg atgacccccc cgcggtgatc gaggcggtcg ccgccgcgtc cgtcggggcg
     1021 acccaagacc gcctcgacgg cctcaccgtc gaccacggcg actggtggtt caacctgcgc
     1081 tccagcaata ccgagccgct cctgcgcctc aacctcgagg ccgccgacga ggcgtcctgc
     1141 cgccaacacc gcgccgagat cctcgcgctc ctcggcggcg aggcagactg agcgctgtga
     1201 ctctcgatcc gaaactgctc gacatcctgg cctgcccgga agacaaaggg cccctgctgt
     1261 acttccccga cgaatcgtcg ctgtacaacc cccggctcaa gcgtcgctac gccgtccggg
     1321 acgacatccc gatcatgctc atcgacgaag ccgagaccgt cagcgacacc gaacacgccc
     1381 ggctactcgc caaagccgac gctgaaggca tccaaccgac cttcgaggcg tagccccggt
     1441 gcccgtcgat tcgctcgggt tactcggcgt cgtcgagggt ctcccggagc agctcgccgc
     1501 ggcccacgag caggccgcca tgaccctcga ccggactgcg ctgccccacc cggaccagat
     1561 cgacaacatc gcggtcctcg gcatgggcgg ttccgggatc gccggcgacg tgctccgggc
     1621 ggtggccacg tcgctgcccc tccccgtcat cgtgctcaag cagatccgca ccccggcctt
     1681 cgtcggacct cgcacgctgg cgttcgcggt ctcctactcc ggtaacaccg aggagaccaa
     1741 cgccatggcc gaaggcgccc tcgacgctgg cgcccgcgtg atcgcggtca ctgctggtgg
     1801 cgagctcgcc aggctcgccg cggcgcgcgg cgcgattcag gtccagtgcg cgggcggcta
     1861 cgtcatgccc cgcgccgcgg tcggcgcgct cgtcgcaccc ctcttcgtca ccctgttcca
     1921 cattggcctg taccccgaag cccacgcgct gctcgtccag gcccaagcgc agctgaagca
     1981 ccggtgcgac cagtgccgac ccgacgccgg ccccggcaac cccgcacgcg agctggcgcg
     2041 acgcatcgcc cgcagcatcc cgctcatcta cggcggtggg gccctcggcg gtgtcgccgc
     2101 ctaccgatgg aagtgcgaca tcaacgagaa cgccaaagcc cccgcgttca ccgccgcgta
     2161 ccccgaactc gaccacaacg agatctgcgg gtggggccag catggggatg tcacccgcca
     2221 gatcatgacg ctcgtgtcgt tgcgacacgg cttcgaggac gagcgcctc
//
LOCUS       DATCCH010000132         7781 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_380940, whole genome shotgun sequence.
ACCESSION   DATCCH010000132 DATCCH010000000
VERSION     DATCCH010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7781
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380940"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..896)
                     /locus_tag="VIJ44_02630"
     CDS             complement(18..896)
                     /locus_tag="VIJ44_02630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEY5164827.1"
                     /translation="MTVADPRARRALWIALGANVGLLVVEVVGGFLFSSLALLADATH
                     LATDVVGLAVALVAQLLVTRPASRRRTFGLRRAEALGAQANGLLVLGVAVWIAVEAAQ
                     RLGHGHPVDGAGLLGVATLGLVVNAVATVVLARVAGKNLNLRAAMVHMLADVASSLGA
                     MVAGIGILAFGATWLDPAASLGIAALVAWSAWGVLRDTTNVLLEAAPRGVDVDQVEQA
                     LFGEAGVEAVHHLHVWEVASDLPALSAHIVLGEVPSLHDAQVRGDELKTMLADRFGIA
                     HTTLELECHECDDPTH"
     gene            993..1763
                     /locus_tag="VIJ44_02635"
     CDS             993..1763
                     /locus_tag="VIJ44_02635"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5164828.1"
                     /translation="MRLAGEIALVTGSTAGIGKAIALEFAEQGAAVCVHGRDAARGGA
                     VVDQIVSSGGDAHFVAADLSTEDVCSDVVRATADRFGGLSILVNNAVASPDGHDARVG
                     EMDTAYWEDALRVNLTAPMWLCRAAIPHLLRGGHGAIVNISSRQAERPSAGLAAYAAS
                     KGGLNALTRAVAVEYAAQGIRCNTISPGYVISERRDADLAPDRRRRLEAMHLTRLGEA
                     RDIAWAAVYLASRESEFVTGINLELDGGSGHARAATLG"
     gene            1760..2752
                     /locus_tag="VIJ44_02640"
     CDS             1760..2752
                     /locus_tag="VIJ44_02640"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY5164829.1"
                     /translation="MISDAELATACAWVTGEDERHVAVTDDGWSLALYRYRGSGPPVV
                     CGHGLAGSRFIFDVHPDYSIARYLAAHGHDVWLVDLRGRGDSWPDGPRGQLQWSFDDF
                     VFRDVPAVCARVLDVTGASEVAWIGTEMSGQVGYAAVISGTAPLRRVVTMGSPVVTPA
                     HAQVPGVTTPYPERDGNRYRFSMVQAIGARLAEQQSEVLASSFRPDDTDWVVTARYFT
                     HGVPDEATDLIDQFADWVETATMRSRDRSVVWSERLDEFTVPVLVAVGAADLQRPASA
                     VRATFDALGSADKQLVVAGTEAGWPVDAGHDDLLCGLAAPTHLYPLLDRFLHAS"
     gene            2752..3558
                     /locus_tag="VIJ44_02645"
     CDS             2752..3558
                     /locus_tag="VIJ44_02645"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEY5164830.1"
                     /translation="MHPRVSLSAISTFSWTLDQDLAFYEDTGITAIGASLAKLEAAGL
                     DAGARRLHDAGLRITNLIGIGPFVLSDPPQWERQRDRVRRALDAAETVGAECLILTTG
                     PAGALSWEDAADALASALGPVVDDAGARGIPLGLEHTNSLRVDVGFVHTLRDVVDLAR
                     RLDIGVCVELNACWAERGLAATIRDAVDTFRIVQVSDFAVGTLSTPNRLVPGDGDIPL
                     GRLLGQVLAARYSGVFDLELIGPRIDEEGYAAASRRAVDRLGALLDQAGA"
     gene            complement(3566..4627)
                     /locus_tag="VIJ44_02650"
     CDS             complement(3566..4627)
                     /locus_tag="VIJ44_02650"
                     /inference="COORDINATES: protein motif:HMM:NF021599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2332 domain-containing protein"
                     /protein_id="HEY5164831.1"
                     /translation="MSVDARTKLGARLRGQAAYCAAACSPFYADLLECLAADAEGGGP
                     TWDLLGRTVDDPALQVPQLRLLGAVHRLVLGGAAPELTAHYPSVGGDADAAAAWPAVR
                     SLFTARHDELARSLDRPPQTNEVGRSAALIGGFLTVSAQTGHPLRILEVGASAGLNLR
                     FDHFWYQAGEHGLGDPASLVRFVDVWEGHPPPFDATMTVSSRAGCDHNPIDPASESGR
                     LALLGYVWPDQAARVALLRGALDVAARVPANVDQADAVEWVAAQLAQPHADEATVVFH
                     SIVMQYFGVDDISEFAAVLADAGARATASAPLAWLRLEPTSTTEVTRAELRLTLWPEG
                     EERLLALAGFHGTPVEWRA"
     gene            complement(4624..5208)
                     /locus_tag="VIJ44_02655"
     CDS             complement(4624..5208)
                     /locus_tag="VIJ44_02655"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEY5164832.1"
                     /translation="MSEARGRDADWRNPWPVAGVRPGEPAPSPGVSALRAEGFTVVFD
                     VDPVDADRDEYQDHLNNTAAARMFNELRTAYVASRLAPRWPKFVRRQGMTVVVRELHV
                     LYEREGWMHERYVGATRYVQRRGKAAIVEQRLVEATGAASLAQAWIVQLLVGSDGRVT
                     TWPPWFWEMLHEVQGAPVEIVDDADRLPWGPPAP"
     gene            complement(5213..5998)
                     /locus_tag="VIJ44_02660"
     CDS             complement(5213..5998)
                     /locus_tag="VIJ44_02660"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5164833.1"
                     /translation="MLHPEVSPEATRAMHRVDGRVIIVTGSGQGVGRGMANHLAKAGA
                     TVVVADYQADKLRRTCDELSELGAPNLGVEVDIRDEESIDAMVAHVADELGRVDGIIN
                     NAQTFRANAPMAEVVADDVDVFYRSGVLGTLRAMQAVYPHMKAAGWGRIVNFASSMGR
                     TGGQGFGAYNASKEAIRALTRTAAREWAADGIVVNAIAPAAAPPRAVGSEHYEEFMRN
                     CPMGRNGDPELDIGPVAHFLCSDACRFVTGHTFMVDGGTFMWS"
     gene            complement(6010..6471)
                     /locus_tag="VIJ44_02665"
     CDS             complement(6010..6471)
                     /locus_tag="VIJ44_02665"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5164834.1"
                     /translation="MPRQLHHAALCVRDVDASLRFYRDGLGLDVLMDHRFEGDWPTLF
                     DVSSRHLRSVFLGDPASADAGIVELVVFDDALNEPESPVIAAGSPGSGFFLLSFFVDL
                     DVTLNRLRGLGFAEHPRRIEQPGPLGPVAMATVRDPDGVLVELIHAKEPGA"
     gene            6497..7384
                     /locus_tag="VIJ44_02670"
     CDS             6497..7384
                     /locus_tag="VIJ44_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019546630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY5164835.1"
                     /translation="MWFGAFLPQLRMGFDRILERVLAAEAAGFDSVWLMDHLAAPAAV
                     DQDTFEGWTLAAALAARTTTIRVGHLVTCDPFRHPALLAKMAATVDVVSGGRLELGLG
                     WGSLAEELRRFGIGEGSASVRAARLGESIEIVRLMFAGEFFDFDGDHFHLEQAIGRPR
                     PVQNHVPIHLGGAGATLTMPLVRDHADWWNCPSYAVDRLAELRPLAGGSRVSVQHPVG
                     LVTEASDRDAVEEQARRRFGAWGGLLVGTPSELVDTLGAEAQLGVEGFVLQFSDFGTP
                     ETLEHFMRSVAPALRASAA"
     gene            complement(7341..>7781)
                     /locus_tag="VIJ44_02675"
     CDS             complement(7341..>7781)
                     /locus_tag="VIJ44_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164836.1"
                     /translation="RSERGVINGARETGANRPRPASSRFVTAVARSALARDAAFVVAG
                     LHAGALVAWGHSALVVAYLVLVVPVALAAAALARRDQREVLDEAGLVMPRRVIDPISP
                     VLGLQAVEAPAAIVEALRGWHAGRVPSPRRVTQRMLAGRGPRYA"
BASE COUNT         1148 a         2727 c         2803 g         1103 t
ORIGIN      
        1 gcggtcgctg agccagctca gtgggtcggg tcgtcacatt cgtgacactc gagctcgagc
       61 gtcgtgtgcg cgatcccgaa ccggtcggcg agcatggtct tgagctcgtc gccgcggacc
      121 tgagcgtcgt gtagcgacgg cacctcaccg agcacgatgt gcgccgacag cgcgggaaga
      181 tccgatgcga cctcccagac gtgaaggtgg tgaaccgcct cgacaccagc ctcgccgaac
      241 aaggcctgct cgacctggtc gacatcgacc ccgcgtggcg ccgcctcgag caacacgttc
      301 gtcgtgtcac gcaacacacc ccaagccgac caggccacga gcgcggcgat cccgagcgac
      361 gccgcggggt cgagccaggt cgcgccgaac gcgaggatcc cgatccccgc caccatcgcc
      421 ccgagcgagc tggcgacgtc ggcgagcatg tgcaccatcg cagctcgaag gttcaggttc
      481 ttgcccgcga cgcgcgcgag caccacggtg gcgaccgcgt tgacgacgag gcccagggtg
      541 gccacgccga gcagcccggc cccgtcgacc gggtggccgt ggccgagccg ctgcgccgct
      601 tcgaccgcga tccagaccgc gacaccgagc accaacaagc cgttcgcctg ggcaccgagc
      661 gcttccgcgc gacgaagccc gaacgtccga cgtctcgagg cgggtcgcgt cacgagcagc
      721 tgggcgacca gcgcgaccgc gagcccgacc acgtccgtcg ccaggtgggt ggcgtcggcc
      781 agcaacgcca acgacgagaa caagaagcca ccgacgacct cgaccaccag cagcccgacg
      841 ttggcgccca aggcgatcca gagcgcccgg cgcgcccgcg ggtccgcaac ggtcatcgcc
      901 cctatcttgg cccgagaaca ccgctagccg ggcaccgctc gtcgcgccag cgacccgctg
      961 gttccgacgc gccgaccggt agggtgccgc tcgtgcgcct cgccggggag atcgcgctcg
     1021 tgaccggctc gacggccggg atcggcaagg ccatcgcgct cgagttcgcc gagcaaggcg
     1081 ctgcggtgtg cgtccacggt cgcgacgcgg cgcgtggcgg cgccgtagtc gaccagatcg
     1141 tcagttccgg cggggacgcc cacttcgtcg ctgcggacct gtcgaccgaa gacgtctgct
     1201 ccgacgtcgt ccgagcgacc gccgaccgct tcggtgggct gagcatcttg gtgaacaatg
     1261 cggtcgccag ccccgacggt cacgacgccc gagtcgggga gatggacacc gcctattggg
     1321 aggacgccct ccgggtgaac ctcaccgcgc cgatgtggct ctgccgcgcc gcgatcccgc
     1381 atctgctccg aggcggtcac ggcgcgatcg tgaacatctc ctcacgacaa gcggagcgcc
     1441 ccagcgcggg tttggccgcc tacgccgcga gcaagggcgg actgaacgcg ctgacgcggg
     1501 ccgtagccgt ggagtacgcg gcgcagggca tccggtgcaa cacgatcagc cccgggtacg
     1561 tgatcagcga gcgacgcgac gccgacctgg cgccggatcg gcggcggcgc ctcgaagcca
     1621 tgcatctcac gcgtctcgga gaagcccgcg acatcgcctg ggccgcggtg tacctggcca
     1681 gccgcgaatc ggaattcgtc accggcatca atcttgaact cgacggcggc agcggccacg
     1741 ctcgcgccgc gacgctggga tgatctccga cgccgagctc gccacggcct gcgcgtgggt
     1801 aacgggagag gacgaacgcc acgtcgcggt caccgatgac ggttggagcc tggcgctcta
     1861 ccggtatcgc ggttcgggac caccggtcgt gtgcggccac ggcctggccg ggagccgctt
     1921 catcttcgac gtccatcccg actattccat cgcccggtac ctcgccgccc acggtcacga
     1981 cgtgtggctc gtcgacctcc ggggccgcgg ggacagctgg cccgacgggc cccgcggcca
     2041 gctgcaatgg tccttcgacg acttcgtgtt ccgcgatgtt ccggcggtgt gtgcccgggt
     2101 gctcgacgtc accggcgcca gcgaggtggc ctggatcggc accgagatga gcggccaggt
     2161 ggggtacgcc gcggtgatca gcggtaccgc accgctgcgg cgggtggtca cgatgggatc
     2221 gccggtcgtc acgccggcac atgcgcaggt gcccggcgtc acgacgccgt atcccgaacg
     2281 agatggcaac cggtaccggt tttcgatggt gcaggccatc ggagcccggt tggcggagca
     2341 gcaatcagag gtgctggcga gcagcttccg tcccgacgac accgactggg tcgtcacggc
     2401 ccgctacttc acccacggcg tccccgacga ggccaccgac ctcatcgacc agttcgccga
     2461 ctgggtcgag accgcgacga tgcggtcccg cgaccggtcc gtggtgtggt ccgagcgtct
     2521 cgacgagttc accgttcccg tcctggtcgc ggtcggcgcc gctgatctgc aacgtccggc
     2581 ttccgcggtg cgggccacgt tcgacgcgct gggcagcgcg gacaagcagc tggtcgtggc
     2641 cggcaccgaa gccggctggc ccgtcgatgc cggccacgac gacctgctct gcgggctggc
     2701 ggcaccgacc catctgtatc cgctgctcga ccgcttcctc cacgcgtcgt gatgcaccca
     2761 cgagtgtcgc tgagcgcgat ctcgacgttc tcgtggaccc tcgatcagga cctggcgttc
     2821 tacgaagaca ccgggatcac ggcgatcggc gcttcactcg ccaagctcga ggccgccggg
     2881 ctcgacgccg gcgcccgtcg gctgcacgac gcggggctgc ggatcaccaa cctgatcgga
     2941 atcgggccgt tcgtgctctc cgaccccccc caatgggaac ggcaacggga ccgggtgcgc
     3001 cgggcgctcg acgccgccga gaccgtcggc gctgagtgcc tgatcctcac gaccggcccg
     3061 gctggcgcct tgtcgtggga ggacgccgcg gacgcgctgg cgtcagccct cggaccggtc
     3121 gtcgacgacg ccggggcgcg cgggatcccg ttgggtctcg aacacaccaa ctcgctgcgc
     3181 gtcgatgtgg gcttcgtgca taccttgcgc gacgtcgtgg acctggctcg ccggcttgac
     3241 atcggtgtct gcgtcgagct gaacgcctgc tgggcggagc ggggactcgc ggcaacgatc
     3301 cgcgacgccg tcgacacgtt ccggatcgtc caggtgagcg acttcgcggt cgggacgctc
     3361 tcgacgccga accggctcgt acccggtgac ggagacatcc ccctcggtcg cctcctgggt
     3421 caggtgctcg cggccagata ttcgggcgtg ttcgaccttg agctcatcgg gcctcgcatc
     3481 gacgaggagg gttacgccgc ggcgagccgc cgggcggtcg accggctcgg tgcgctcctc
     3541 gaccaggccg gcgcctgaac gcggctcagg ccctccactc caccggcgtc ccatggaacc
     3601 cggcgagggc caggagccgc tcctcaccct caggccaaag cgtgagtcgc agctcggcgc
     3661 gagtcacctc ggtcgtcgag gtcggctcaa gccgcaacca cgccagcggc gcggacgcgg
     3721 tggcgcgggc accggcgtcc gcgaggacgg ccgcgaactc cgagatgtcg tccacgccga
     3781 agtactgcat gacgatggag tggaagacga ccgtggcttc gtcggcatgg ggttgggcga
     3841 gctgcgcggc gacccattcg acggcgtccg cttggtcgac gttggccggc acccgggccg
     3901 cgacgtcgag cgcgccgcgc aggagcgcga cccgggcggc ttggtcgggc cagacgtagc
     3961 cgagcagcgc gaggcgcccc gattcgctcg ccgggtcgat cggattgtgg tcgcagccgg
     4021 cgcgcgacga cacggtcata gtggcgtcga acgggggtgg atgcccctcc cagacgtcga
     4081 cgaaccgcac cagcgaagcg gggtctccga gcccgtgctc ccccgcctgg taccagaaat
     4141 ggtcgaaccg caggttcagg cctgcgctcg cacccacctc gaggatgcgc agcgggtggc
     4201 cggtctgcgc cgagacggtc aggaacccgc cgatcaacgc ggccgatcgg ccgacctcat
     4261 tggtctgggg tggtcggtcg agtgaccggg cgagctcgtc gtgtcgcgcc gtgaacaagg
     4321 accgcaccgc cggccaggcc gcggcggcgt cggcgtcgcc accgaccgag gggtagtgcg
     4381 cggtgagctc gggggcggcg ccgccgagca cgaggcggtg caccgcgccg aggaggcgga
     4441 gctgggggac ctggagcgcg gggtcgtcga cggtgcggcc gaggagatcc caggtgggcc
     4501 ccccgccctc ggcgtcggcg gcgaggcact cgagcaggtc ggcatagaac ggggagcagg
     4561 ccgcggcgca gtacgccgcc tggccccgca ggcgtgcacc gagtttcgtc cgggcgtcga
     4621 cgctcacggt gccggtggtc cccacggcag acggtcagca tcgtcgacga tctcgacggg
     4681 tgcaccttgg acctcgtgga gcatttccca gaaccacggc ggccaggtgg tgacgcgccc
     4741 gtcggacccg acgagcagct ggacgatcca agcttgggcg agcgacgccg cgccggttgc
     4801 ctccacgagg cgctgctcga cgatggccgc cttccctcgc cgctgcacgt accgggtggc
     4861 cccgacgtat cgctcgtgca tccacccttc acgttcgtac aggacgtgga gttcgcgcac
     4921 gacgaccgtc atgccctggc gtcgcacgaa cttcggccag cggggcgcga gccgcgacgc
     4981 cacgtacgcg gttcgcagct cgttgaacat gcgcgccgcg gcagtgttgt tgagatggtc
     5041 ctggtactcg tcccggtcgg cgtcgacggg gtcgacgtca aacacgacgg tgaacccctc
     5101 ggcgcgcagc gccgacacgc cgggcgatgg ggctggttcg ccgggacgga ccccggcaac
     5161 gggccacggg ttcctccagt cggcgtctcg tcctcgcgcc tcgctcaacg ggctaggacc
     5221 acatgaaggt tccgccgtcg accatgaagg tgtggccagt cacgaagcgg cacgcatcgg
     5281 agcacaagaa gtgggccacc gggccgatgt cgagctccgg atctccgttg cggcccatgg
     5341 ggcagttccg catgaactcc tcgtagtgct ccgatccgac ggcgcggggc ggagcggcgg
     5401 cgggagcaat ggcgttgacc acgatcccgt ccgcggccca ctcccgggct gcggtgcggg
     5461 tcaaggcgcg gatggcctcc ttcgaggcgt tgtaggcccc gaagccctga ccgccggtgc
     5521 gtcccatcga cgacgcgaag ttgacgatgc gaccccagcc ggcggccttc atatgcgggt
     5581 agacggcctg catggcgcgc agggtgccca gcaccccgga gcggtagaac acgtcgacgt
     5641 cgtcggcgac gacctccgcc atgggtgcgt tggcgcggaa ggtctgcgcg ttgttgatga
     5701 tcccgtcgac ccggccgagc tcgtcggcga cgtgcgccac catggcatcg atcgactcct
     5761 cgtcgcggat gtcgacctcg accccgaggt tgggggcgcc gagctcggag agctcgtcgc
     5821 aggtgcgtcg cagcttgtcg gcctgatagt cggcgaccac gaccgtcgca ccggctttgg
     5881 cgagatggtt cgccatcccg cgtccgacgc cctggccgga accggtgacg atgatgaccc
     5941 gcccgtcgac gcggtgcatc gcgcgggtgg cctcgggcga gacttcgggg tggagcacga
     6001 tccgggaggc tacgccccgg gttccttcgc gtggatcagc tcgacgagga caccgtcggg
     6061 atcacggacg gtcgccatcg cgaccggccc gagcggaccc ggctgctcga tccgccgggg
     6121 gtgctccgcg aagccgaggc ctcggagccg gttgagggtg acgtcgaggt cgacgaagaa
     6181 cgacaacaag aagaagcccg agccgggcga ccccgccgcg atgaccggac tctcgggttc
     6241 gttgagcgcg tcgtcgaaga ccacgagctc cacgatcccg gcgtcggcgc tcgccgggtc
     6301 accgaggaac accgatcgga ggtgacgcga cgacacgtcg aacagcgtgg gccagtcgcc
     6361 ttcgaaccgg tggtccatca ggacatcgag tccgagaccg tcgcggtaga agcgcaggct
     6421 ggcgtcgacg tcgcgtacgc acaacgcggc gtggtgcagc tgtcgtggca cgcggccgac
     6481 gctaccctcc tgcgcgatgt ggttcggagc cttcttgccc cagctgcgca tgggattcga
     6541 ccggattctg gagcgcgtcc tcgcggcgga ggccgcgggg ttcgattccg tctggctcat
     6601 ggatcacttg gcggcgcccg ccgcggtgga ccaggacacg ttcgagggct ggacgctcgc
     6661 ggcggcgctc gcggcgcgga ccacgacgat ccgcgtgggc catcttgtga cctgcgaccc
     6721 gtttcgccac ccggcgttgt tggcgaagat ggcggcgacg gtcgatgtgg tgagcggggg
     6781 gcgtctcgag ctgggattgg gctgggggtc actcgccgag gagctgcggc gattcgggat
     6841 cggagagggg tcggcgagcg tccgcgccgc gcggctggga gagtcgatcg agatcgtgcg
     6901 gttgatgttc gccggcgagt tcttcgattt cgacggggac cacttccacc tcgagcaggc
     6961 gatcggccgg cctcggccgg tgcagaacca cgtgccgatc cacctcggag gcgcgggggc
     7021 gacgctgacc atgccgttgg tccgcgacca cgccgactgg tggaactgcc cgtcctacgc
     7081 ggtggatcgg ctcgcggagt tgcggcccct ggcgggcggt tcgcgggtgt cggtgcaaca
     7141 cccggtcggg ctggtcaccg aggcgtcgga ccgtgacgcc gtcgaggagc aggcccgccg
     7201 gcgcttcggc gcctgggggg gcctcctcgt cgggaccccg tcggagctcg tcgacaccct
     7261 cggcgccgag gcccagctcg gggtcgaagg cttcgtgctc cagttctcgg acttcgggac
     7321 ccccgagacg ctcgagcact tcatgcgtag cgtggccccc gccctgcgag catccgctgc
     7381 gtgacccggc gcggcgaggg gacgcgaccg gcgtgccacc ctcggagtgc ctcgacgatc
     7441 gccgccggcg cttcgacggc ctgcaggccg agcaccggcg agatcggatc gatgacccga
     7501 cgcggcatga ccaggcccgc ttcgtcgagc acctcgcgct ggtcgcggcg ggccagcgcc
     7561 gccgccgcca acgcgacggg gacgacgagc accaggtagg cgacgacgag cgcgctgtga
     7621 ccccaggcga ccagcgcgcc agcgtggagc ccggcgacta cgaaggccgc gtcacgggcg
     7681 agcgccgacc gggccacggc ggtcacgaac cggctcgacg cgggccgcgg acggttggct
     7741 ccggtctcgc gggcgccgtt gatgacgccg cgctcagaac g
//
LOCUS       DATCCH010000133         5514 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_553558, whole genome shotgun sequence.
ACCESSION   DATCCH010000133 DATCCH010000000
VERSION     DATCCH010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5514
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553558"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /locus_tag="VIJ44_02680"
     CDS             complement(<1..805)
                     /locus_tag="VIJ44_02680"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEY5164837.1"
                     /translation="MSEMGRRREELRALVGRLRGVVFWSAVTGLLTGLVVTGFEHLVL
                     DEALGPVLGLPIWATAMLPMMGLAVAFAVLRVVGGGASPATSDAYLQAFHGQADLGWR
                     PSVARLLASVATIGSGGALGLEGPSQYAGAAIGQGLEGGRGSRVGSQRRSLLVAGAAA
                     GVGAIFKAPATGAVFALESPYQDDLARRMLLPALVGAATGYLVLVSFDGTTPLLTVHG
                     SPPFSGVDLAGAVAIGFLAGIAARLFAALVRAAKHVAARRPALPRIVLGG"
     gene            complement(798..1586)
                     /locus_tag="VIJ44_02685"
     CDS             complement(798..1586)
                     /locus_tag="VIJ44_02685"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5164838.1"
                     /translation="MRAQRVWAITRRQGYVLKRAPQRWFDVIVWPVVDAILFGAIGVY
                     FGRQHGTAAASAAFLLAGIVLFHVVFQAEISLATGFLEETWSRNLLNLMVTPLTELEY
                     GAGVVLFGLVRLAIGVSVVSIVAFALFAFDITTLGLALIPLVVILLVVGWSVALLVIG
                     LILRVGQGAEILTWGVIAFLLPMSGIFYPVSALPGGLQPVAEALPTTHVFAAGRAVEA
                     GHSLPWGQVGLAALSAVALVALSVWFLTRMLATFRRRGFISRHV"
     gene            complement(1586..2353)
                     /locus_tag="VIJ44_02690"
     CDS             complement(1586..2353)
                     /locus_tag="VIJ44_02690"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5164839.1"
                     /translation="MAEPETALAARGLIKQFRRIRAVDGIDLTVGAGERVALLGPNGA
                     GKTTTLMMLLGVITPDEGEIEIVGRKLPRERSAAMEQVGFAAGYLPLPERLRVREFLE
                     LFADLYGLRDGRAAVARGLERFRIEGLADIMGNELSSGQRTLVGIVKAGLHQPKLLVL
                     DEPTASLDPDVAHRVRSGLERLCAEDGTALLVTSHNMQEVERLCTRVVFVAGGRIVAD
                     GTAEAVAARFGRADLEGVFLHLAESSAETISDARVVP"
     gene            2421..2840
                     /locus_tag="VIJ44_02695"
     CDS             2421..2840
                     /locus_tag="VIJ44_02695"
                     /inference="COORDINATES: protein motif:HMM:NF014584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HEY5164840.1"
                     /translation="MRARSEAILVALGGALGAGARYELLQTFPVHPGRFGLTTGSINV
                     GGAFLLGFLLEWLTRHRDVDHWARWLVGVGGLGAFTTFSAFAAFSVELVLLARDGHGV
                     LAFADAGVSLVAGVAVVFVGLVAAGWQRAPVPSEAES"
     gene            3034..3189
                     /locus_tag="VIJ44_02700"
     CDS             3034..3189
                     /locus_tag="VIJ44_02700"
                     /inference="COORDINATES: protein motif:HMM:NF014584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164841.1"
                     /translation="MGTGFLGAYTTFSTYAYEVVRRGEEGQAPVAGSTRWSAWCSVWS
                     SRPRASP"
     gene            3273..4475
                     /locus_tag="VIJ44_02705"
     CDS             3273..4475
                     /locus_tag="VIJ44_02705"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018501211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY5164842.1"
                     /translation="MGVLDGVRVVELAEHGFVPSCGAILGDWGAEVIKIERPEGDPLR
                     TVMAAGLVADTGDVNFLWELYNRNKRGLALDLRVAAGRAVFDRLLEDADVLLTSFLPS
                     ARHKLGVEPEDCWAVNPRLIYAKGHGQGQRGPDADQGGFDAVSFWARGGLGHILSPPQ
                     GPMIMQRGAMGDAPSGAMLAGGVAAALFQRERTGRGMVVDVALLNTAVWQLSMDLTAT
                     AVLGENPRPLHATGVLPNPLVGPYRTGDGRWLLLNMLDDTRHFAPACRALGLDALLDD
                     PRFADTAGRAEHRIELHELIAERIAASALADLRSGLQREDTIFAALASPLEVIHDEQV
                     IANGYFAPHPTRPSVRLACAPMQFDDEMTEIRRAAPETGEHSDEVLAELGYDADAIRA
                     LRSAGAVA"
     gene            4505..5395
                     /locus_tag="VIJ44_02710"
     CDS             4505..5395
                     /locus_tag="VIJ44_02710"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HEY5164843.1"
                     /translation="MPDGLTWDQTPERLRQPVLVAGFEGWNDAADAASTAAGWLTQHG
                     TSTPLASIDPEEHFDFQSRRPQVELVDGVTRKIAWPENHFFSVGFDDRDLVVLRGIEP
                     SYRWKSFSRAVTTVAHETGCEMVVTLGALLADVPHTRAARITGTATDGELIDRLGLAQ
                     SRYEGPTGIVGVLNDYCRTDGVPSVSLWAPVPHYLAAPPNPPATLALLERFAGLAGLN
                     FDLTRLQQTAQAWREKVDEVAAGDDDVRNYVSTLEERFDAQTTADGSISDQSWGTNLP
                     SGDELAREVERFLREQRDDS"
BASE COUNT          826 a         2047 c         1894 g          747 t
ORIGIN      
        1 cgccgccgag cacgatccgg ggcagcgccg gacggcgcgc cgcgacgtgc ttcgcggccc
       61 gcacgagcgc ggcgaacagc cgagccgcga tcccggcgag gaacccgatc gcgaccgcgc
      121 ccgcgaggtc gacgccggag aacggcgggc tgccgtggac ggtcagcagc ggcgtcgtcc
      181 cgtcgaacga caccaagacc aggtagccgg tggcggcgcc gaccagcgcg gggagcagca
      241 tgcggcgggc caggtcgtcc tggtacgggg actccagtgc gaacaccgcc cccgtcgccg
      301 gagccttgaa gatcgccccc acccccgccg cggcgccggc gacgagcagc gatcgccgct
      361 gcgacccgac ccgcgacccc cgtccaccct cgaggccttg tccgatcgcg gccccggcgt
      421 actgcgacgg gccctccaga cccagcgcgc cgccggaccc gatcgtcgcc accgacgcca
      481 gcagccgcgc cacgctcggc cgccacccga ggtcggcttg cccgtggaac gcctgcaggt
      541 acgcgtccga cgtcgcgggg ctcgccccgc cgccaaccac ccgcagcacc gcgaacgcca
      601 ccgccaaccc catcatcggc agcatcgccg tcgcccagat cggcaacccg agcaccgggc
      661 ccagcgcctc gtcgagcacc agatgctcga acccggtgac cacaaggccg gtcaggagac
      721 cggtgaccgc cgaccagaac acgacgccgc gcagccgccc gaccagcgct cgcagctcct
      781 cgcgtcggcg gcccatctca gacatggcgg ctgatgaagc cacggcggcg gaacgtcgcc
      841 agcatgcggg tcaggaacca caccgagagc gcgacgagcg ccaccgcgct gagcgcggcg
      901 aggccgacct gaccccaggg gagcgagtgg cccgcttcga cagcccgacc ggcggcgaac
      961 acgtgggtcg tcgggagcgc ctcggccacg ggctgcaacc cgccgggaag ggcactcacc
     1021 gggtagaaga tccccgacat cggcaacagg aacgcgatga ccccccaggt gaggatctcg
     1081 gcgccctgcc cgacccgcaa gatgagaccg atgacgagca gcgccaccga ccagccgacg
     1141 accaggagga tcacgaccag cgggatcagc gccagcccga gcgtcgtgat gtcgaacgcg
     1201 aacaacgcga acgcgacgat cgacacgaca ctgacgccga tggcgaggcg caccaggccg
     1261 aacaggacga ccccggcccc gtactcgagc tcggtgagcg gcgtgaccat caagttgagg
     1321 aggttacggg accacgtctc ctccaagaag ccggtcgcga ggctgatctc ggcttggaag
     1381 accacgtgga acagcacgat cccggcgagg aggaaggcgg cgctggccgc cgcggtgccg
     1441 tgctgacgtc cgaagtacac gccaatggca ccgaagagga tggcgtcgac gaccggccac
     1501 acgatcacgt cgaaccaccg ctgcggcgcc cgtttgagca cgtagccctg gcgtcgggtg
     1561 atggcccaga ctcgttgggc gcgcatcacg gcaccacccg ggcgtcgctg atcgtctcgg
     1621 cgctggactc ggcgaggtgc aagaagaccc cttcgaggtc cgcccgaccg aaccgggccg
     1681 cgacggcctc agcggtcccg tcggcgacga tgcggccccc ggcgacgaag accacccgag
     1741 tgcagagccg ctccacctct tgcatgttgt ggctggtgac gagcaacgcg gtgccgtcct
     1801 cggcgcagag ccgctcgagg ccgctgcgta cccggtgggc gacgtcgggg tccagcgacg
     1861 cggtgggctc gtcgagcacg aggagcttcg gctggtgcag cccggccttg acgatcccga
     1921 cgagggtccg ctgccctgac gacagctcgt tgcccatgat gtcggcgagc ccctcaatgc
     1981 ggaaccgctc cagcccgcga gcgaccgcgg cccgcccgtc tcggagaccg tagaggtcgg
     2041 cgaacagctc gaggaactcc cggacccgga gccgttccgg gagtggcagg tagccggccg
     2101 cgaagccgac ctgctccatc gccgcgctgc gctcgcgcgg caacttccgg cccacgatct
     2161 cgatctcgcc ctcgtcgggg gtgatgaccc cgagcagcat catcaacgtg gtggtcttcc
     2221 ccgcaccgtt cggtcccagg agcgcgacgc gttcgccggc gccgaccgtg aggtcgatgc
     2281 cgtccacggc gcggatgcgc cggaactgct tgatcagacc gcgcgcggct agcgcggtct
     2341 ccggctcggc catgggttca gtgtcgcaga gtctcggcgg gtgctggtcg tggtttccgg
     2401 ccggctcata ggctcgcccg gtgcgtgccc ggtccgaggc gatcctggtc gctctcggcg
     2461 gcgcgttggg cgccggggcc cggtacgagc tgctgcagac gttcccggtc catccgggac
     2521 gcttcgggct caccaccggc tccatcaacg tcggtggcgc gttcctcctc ggattcctcc
     2581 tggagtggct gacccgccac cgcgacgtgg atcactgggc gcgctggctc gtgggcgtcg
     2641 gcggtctcgg cgcgttcacg acgttctcgg cgttcgcggc gttctcggtc gagctcgtgc
     2701 tcttggcccg cgacggccac ggagtcctcg cgttcgccga cgcgggggtg tccctcgtcg
     2761 ccggggtcgc ggtggtgttc gtcggtctcg tcgctgccgg ttggcagcga gccccggtcc
     2821 cgagcgaggc cgagtcgtga ccctgctcgg cgtcctcgtc gccggtgcgc tcggtgcgcc
     2881 ggtccggtac ctgctcgagc gggcgctgcg ccggaccggt caggtgttcc cgtgggggca
     2941 cgttcctcgt gcacctgagc ggctcgttcg cgatcggtgt cgtgacagac cgccgcgctc
     3001 acgcaagggt tctccgccga cgctcggacc atcatcggca ccgggttcct cggggcgtat
     3061 acgaccttct cgacctacgc ctatgaggtc gtacgccgag gtgaggaagg ccaggccccg
     3121 gtggcgggat ctacgcggtg gtcagcctgg tgctcggtgt ggtcttcgcg gccgcgggcc
     3181 tcgccgtgac cggcgcgctc tgacccgtcg tcgccgcgcc ccgccgacag ggaagccaga
     3241 ctgcggtgcg ttccgaccgg agggggtcct caatgggagt actggacggt gtgcgggtag
     3301 tggagctcgc cgagcacggg ttcgtcccgt cctgtggcgc gatcctcggc gactggggcg
     3361 ccgaggtgat caagatcgag agacccgaag gtgacccgct ccgaacggtg atggccgcgg
     3421 gcctcgtcgc cgacaccggc gacgtcaact tcctctggga gctctacaac cgcaacaagc
     3481 gcggcctcgc gctcgatctg agggtcgcgg cgggtcgtgc cgtattcgac cggctcctcg
     3541 aagacgccga cgtgctgctc accagcttct tgccgtcggc gcgccacaag ctcggtgtgg
     3601 aacccgagga ctgctgggcg gtgaaccccc gactcatcta cgccaaggga cacgggcagg
     3661 gtcagcgagg tcccgacgcc gaccagggcg ggttcgacgc cgtgtcgttc tgggctcgcg
     3721 ggggactcgg ccacatcttg agccccccgc aaggcccgat gatcatgcag cgcggcgcca
     3781 tgggcgacgc gccgagcggc gccatgctcg ccggcggtgt cgccgcggca ctgttccaac
     3841 gggagcgcac cgggcgcggg atggtcgtcg acgtggcgtt gctcaacacc gcggtgtggc
     3901 agttgagcat ggacctcacc gcgaccgcag tgctcggcga gaaccctcga cccctccacg
     3961 ccaccggtgt cctccccaac ccgctggtcg gcccgtaccg gaccggcgat ggacgctggc
     4021 tgctcctcaa catgctcgac gacacccgcc acttcgcgcc ggcttgtcgc gccctcgggc
     4081 tcgacgcgct cctcgacgac ccccgcttcg cggacaccgc gggccgagcc gagcaccgca
     4141 tcgagctcca cgagctcatc gcggagcgca tcgccgcgtc cgccctcgcg gacctgcggt
     4201 cgggtctcca acgcgaagac accatcttcg ccgcgctggc ctcgccgttg gaggtgatcc
     4261 acgacgagca ggtcatcgcc aacggctact tcgcgcctca cccgacccgc ccatcggtcc
     4321 ggctggcgtg cgcgccgatg cagttcgacg acgagatgac cgagattcgc cgcgccgccc
     4381 ccgagaccgg cgagcacagc gacgaggtcc tcgccgagct cggctacgac gccgacgcaa
     4441 tccgggcgct gcgctcggcg ggcgcggtcg cgtgactccg gcgggaccga cgtaacgtga
     4501 cggcatgccc gatggcttga cctgggacca gacccccgag cggctgcgcc aaccggtgct
     4561 cgtggccggc ttcgagggct ggaacgacgc cgccgacgcg gcctcgaccg ccgcgggatg
     4621 gctcacccag cacggcacct cgaccccgtt ggcgtcgatc gaccccgagg agcacttcga
     4681 cttccagtcc cgacgacccc aggtcgagct ggtcgacggg gtgacccgca agatcgcctg
     4741 gcccgagaac catttcttct cggtcggatt cgacgaccgt gacctggtgg tgctccgtgg
     4801 gatcgaaccc agctaccggt ggaaatcatt ctcgcgggcg gtgaccaccg tcgcgcacga
     4861 gaccggctgc gagatggtcg tcacgctcgg ggccctgctc gccgacgtgc cccacacccg
     4921 cgccgcgcgg atcaccggca ccgccaccga cggcgagctc atcgaccggc tcggcctggc
     4981 ccagtcacgg tacgaagggc cgaccgggat cgtcggggtc ctgaacgact actgccgcac
     5041 cgatggggtc ccgtcggtgt cgctgtgggc gccggtcccc cactacctcg ccgcgccccc
     5101 caacccgccg gcgacgttgg cgctcctcga gcgcttcgcc gggctcgcgg ggctgaactt
     5161 cgacctgacc cgactgcagc agaccgcgca ggcctggcgc gagaaggtcg acgaagtcgc
     5221 cgccggcgac gacgacgtcc gcaactacgt ctcgaccctc gaggagcgct tcgacgccca
     5281 aaccaccgcg gacgggtcga tctcggacca gtcctggggc acgaacctgc ccagcggcga
     5341 tgagctggcc cgggaggtcg agcggttcct gcgcgagcag cgcgacgaca gttgagcagc
     5401 cgcgtccgga cgcggatcga catcgccgcc ccgatcgacg tggtcttcgc cttcttcgac
     5461 gacctcgcca acgccgccgt cctggtcccc agcctcggcg agatcaccgc cgtc
//
LOCUS       DATCCH010000134         2001 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_422064, whole genome shotgun sequence.
ACCESSION   DATCCH010000134 DATCCH010000000
VERSION     DATCCH010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422064"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VIJ44_02715"
     CDS             complement(<1..199)
                     /locus_tag="VIJ44_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020787042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase"
                     /protein_id="HEY5164844.1"
                     /translation="MTVMKYGQAIDAALGEAMARDDRVVTWGEDVRLIHRDLLARFGP
                     DRVSDTSISESAFLYAGVGAAM"
     gene            complement(196..1263)
                     /locus_tag="VIJ44_02720"
     CDS             complement(196..1263)
                     /locus_tag="VIJ44_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006334434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEY5164845.1"
                     /translation="MGVPPGFDAASAYRAMVRMRLTEEALVQAWADGLVPGEYHSGIG
                     EEGINAGVLAHLDGRDSMALDHRNTSPLIGRGTDLTSLMLEVMGSEDGLNRGRAGHMH
                     LMDPGIPATADGIVGSSGPLAVGQAIANGRLHPGRIAVAFHGEGAMNQGMLMESYSMA
                     VAWNLPVLFVCKDNKWSITTYSAEVTGGDLVERARSFGLAVEKVGGDRVEDVHRAAGR
                     LIARARRGRGPGFLHAGCHRPGGHFEGDPIVRLLRNPRGQAEEWGPPLIQSARTKSGG
                     NRSDHVAAVTVLTKRGARAARDYAVRARMDPVRHGRRLLEPSAAERIEATERLEVEQA
                     MTEARAAIGPRATFGATGGTR"
     gene            complement(1539..>2001)
                     /locus_tag="VIJ44_02725"
     CDS             complement(1539..>2001)
                     /locus_tag="VIJ44_02725"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5164846.1"
                     /translation="VDATFGGYWAWDALTRAIASDETLVWPAGDLGVPRYHSYVLGAP
                     EQLLLGSPDTVRRFLEATSRGFRTAASEPEATRRDLEQAIPYVPSWRLERSLELVAPT
                     WTHDGIWGGLREELLGPYAEWLASYGILRTPSRWSDGFTTEFLPPQGASVL"
BASE COUNT          313 a          719 c          662 g          307 t
ORIGIN      
        1 ccatggcagc cccgacgccg gcgtagagga acgccgattc actgatcgag gtgtcgctca
       61 cccggtcggg cccgaaccga gcgagcaagt cgcggtggat gagcctgacg tcctcgcccc
      121 aggtgacgac acggtcgtcg cgggccatcg cctcgccgag ggcagcatcg attgcctggc
      181 catacttcat gaccgtcatc gggttcctcc ggtcgcgccg aacgtggccc gtgggccgat
      241 cgccgccctg gcctcggtca tggcctgttc gacctccagg cgctcggtcg cctcgatgcg
      301 ctcggcagcc gacggttcca gcagccgccg tccgtgccgc acggggtcca tccgggcgcg
      361 aaccgcgtag tcgcgggcag cccgggctcc gcgcttggtc agcaccgtga ccgctgcgac
      421 atggtcgctg cggttcccgc cggactttgt ccgggcgctc tggatcagcg gcgggcccca
      481 ctcctcggcc tgtccgcgcg ggttgcgcag cagacgcacg atgggatcac cctcgaagtg
      541 gcccccgggt cggtggcatc cggcgtggag gaagccaggg ccccgccccc tgcgagcccg
      601 ggcgatcagc cgtccggcag ctcggtggac gtcctcgacc cggtcgccac ccaccttctc
      661 cacggccagc ccgaacgaac gggcccgctc caccagatcc cctcccgtga cctccgccga
      721 gtacgtcgtg atcgaccact tgttgtcctt gcagacgaac aggacaggga ggttccaggc
      781 gacagccatg ctgtaggact ccatcagcat cccctgattc atggcaccct cgccgtggaa
      841 cgccaccgct atgcgaccgg gatgcagccg cccattggcg atggcctgac cgacggccag
      901 cggaccgctc gagcccacga tcccgtcggc cgtagccggg atacccgggt ccattagatg
      961 catgtgtccg gcccggcccc gattgagccc gtcttccgaa cccatcacct cgagcatgag
     1021 agatgtgagg tcggttcctc taccgatcag aggggaggta ttgcgatggt cgagcgccat
     1081 cgagtcgcgg ccatcgaggt gggccagaac gccggcattg atgccttcct cacctatccc
     1141 cgagtggtac tcgccgggta cgaggccgtc cgcccaggcc tgcaccagtg cctcctcggt
     1201 gaggcgcatc cgcaccatcg cccggtaagc gcttgcggcg tcgaatcctg gaggaactcc
     1261 catgccaacc tcgaagctcg cggctcggat gcccggccga agacggggcg cccgggagtc
     1321 cgagcgcccg acaaccggcc cgagtggccc gggccacacg gaactctagg actgcagatc
     1381 ggtgatcact agggtccaac gaccctttcg tcccctgggt accatttggc gccgatgcgc
     1441 cacctgacgg cctcgactcg agcctttggg cgacccgtga cgggacggtg actacctggg
     1501 cttggtcgac cgccgaaggt gccgcccggc tcagtggatc agagaaccga cgcgccctgc
     1561 ggtgggagga actcggtggt gaagccgtcg gaccacctgc tcggggtgcg gaggatccca
     1621 tacgatgcga gccattccgc atagggcccg agcagttcct cgcggagccc gccccagatg
     1681 ccgtcatggg tccaggtcgg cgccacgagc tccagcgagc gctccaggcg ccacgacggc
     1741 acgtagggaa tggcctgctc caggtcgcgc cgcgtcgcct cgggttccga cgccgctgtg
     1801 cgaaagccgc ggctggtggc ctcgaggaac cggcgtaccg tgtcgggcga cccgaggagc
     1861 agctgttcgg gtgccccgag gacgtagctg tggtagcggg gcacgcccaa atcgcctgcc
     1921 ggccacacca gggtctcgtc ggaagcgatc gcccgggtca aggcgtccca cgcccagtaa
     1981 ccgccgaaag ttgcatcgac g
//
LOCUS       DATCCH010000135         3090 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694 k141_7321,
            whole genome shotgun sequence.
ACCESSION   DATCCH010000135 DATCCH010000000
VERSION     DATCCH010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7321"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..1576)
                     /locus_tag="VIJ44_02730"
     CDS             complement(44..1576)
                     /locus_tag="VIJ44_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY5164847.1"
                     /translation="MLAETLREAARRFGAAPAYVTEEGWALSFEDLDRVSDEVAVGLR
                     ARGVAVGDVLALVLPPGPDYLVAYLAAAKLGAVTAGVNDRLTTTERAPVLERAAPRLV
                     LTGRGLGPAGFDRVEVDAAPSLGDLLGCLRVTGAAPPPLPDDPERAVAIIFTSGTTGI
                     PKGAVYANRQLAFITANDIGDAWGGGGRTFTGTSFAHLGFMTKLAGNLRRGGTSYILR
                     RWSAPRSLELLAEHQMTTVAGVPAQLALMLRQPDFDDYDLDSVRFIVIGGGPVTPGLA
                     DEARTRFGAALATRYSCTEAGIGLGTAFDDPDEDAVVSVGRPHTAVALAVLDDDDRPV
                     SDGAVGAVCLRSPAVMTEYWRDQEATQAAFTRDGFVRTGDLGWVDERGRLHLVGRTKE
                     MYVRGGYNVYPVEVESVLSTHPDVAAVAVVPRPDPVMGEVGVAVVVAREPTSPPTLDA
                     LRAHAGAALAGYKLPEALRIVEALPLTAMDKVDRRVLGHQVVGGPREPSRRGDGTGGE
                     PG"
     gene            1665..2324
                     /locus_tag="VIJ44_02735"
     CDS             1665..2324
                     /locus_tag="VIJ44_02735"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY5164848.1"
                     /translation="MPASYKSSPRREAEASAGLPAQHRELRAQGRRTLRRLLDAGLRV
                     FARRGYHAARVDDIVRAARTSHGTFYLYFANKEDLLRALAVDCAHELTDLAASIGPIG
                     RDASGRTELRAFLERFIDTYRRYGPVIRAWMEDQVGDRDVDRLGVKAFTAIGGRLGER
                     MRDTQVMFESEDAAVGALMAMLERLSYGVASGRITADNETLLDTLTTIVHRGFFAATD
                     P"
     gene            complement(2338..>3090)
                     /locus_tag="VIJ44_02740"
     CDS             complement(2338..>3090)
                     /locus_tag="VIJ44_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011856467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HEY5164849.1"
                     /translation="FDAPYGHALIAKYAMATRRHMYEYGTTIEQLAEIAVSTRFNASL
                     NPDAYHREPISIEDVQSSRLIADPLTKLHCCLRSDGGGAVVLTSEARARDLRHPPVWV
                     LGTGEATSHTTMSEWPDFTDSPARRSGQAAFERAGVTPTDIDCCQLYDAFTPMVLLTL
                     EALGFCSKGDGGGFVEDGRLRVGGSLPTNTDGGGLSACHPGMRGMFLLVEATRQLRGD
                     AGARQVDGAELCCVNGTGGWFSSASTVILAAG"
BASE COUNT          466 a         1088 c         1103 g          433 t
ORIGIN      
        1 cgtgggcccg agcgattcgc aaccagctct tcggtggtcg ccgtcatccc ggttcgcccc
       61 ccgtcccgtc cccgcgacgg gacggttctc gcgggcctcc cacgacctgg tggccgagga
      121 cgcggcgatc caccttgtcc atcgccgtga gcggcaacgc ctcgacgatc cggagagcct
      181 ccggcagctt gtagccggcg agtgctgctc ccgcatgggc acgcagggcg tcgagggtcg
      241 gcggcgacgt cggttcgcgg gctacgacca cggcgacgcc gacctcgccc atcaccggat
      301 ccggtcttgg gacgaccgcg accgcggcga catcagggtg cgtcgacagc acgctctcca
      361 cctcgaccgg gtacacgttg tacccgccgc ggacatacat ctctttcgtg cgccccacga
      421 gatggaggcg gccgcgctcg tcgacccagc cgagatcgcc ggtgcgaacg aacccatcgc
      481 gtgtgaacgc cgcctgggtc gcttcctggt cccgccagta ctcggtcatc acggccggcg
      541 accgcaagca caccgcgccg acggcgccgt cggaaaccgg gcggtcgtcg tcatcgagga
      601 ccgcgagagc gaccgcggtg tgcggacgcc cgacgctcac gacggcgtcc tcatcggggt
      661 cgtcgaacgc ggtgccgagc ccgatcccgg cctcggtgca ggagtaccgg gtggcgagcg
      721 cggccccgaa ccgggtccgg gcctcatcgg ccaagccggg tgtcaccggt ccgcctccga
      781 tgacgatgaa ccgcacgctg tcgaggtcgt agtcgtcgaa gtcgggctgg cggagcatga
      841 gggccagctg ggccgggacg cccgccaccg tcgtcatctg gtgctcggcg agcagctcca
      901 acgagcgagg cgcgctccac cgccgcagga tgtagctcgt ccccccgcgg cgaaggttcc
      961 cggccagctt ggtcatgaac ccgaggtgcg cgaacgacgt gccggtgaag gtgcgtcctc
     1021 ctccgcccca agcgtcgccg atgtcattgg cggtgatgaa cgcgagctgg cggttggcgt
     1081 acaccgcacc tttcgggatc ccggtggtcc ccgacgtgaa gatgatggcc acggcccgtt
     1141 ccgggtcgtc gggcagcggg ggtggtgcgg caccggtgac gcgcaggcat ccgaggaggt
     1201 cgcccagcga aggcgccgcg tcgacctcga cgcggtcgaa ccccgccggt cccagccccc
     1261 gtccggtcag caccaggcgc ggtgcggcgc gttcgagcac cggggcgcgt tcggtcgtag
     1321 tcagccggtc attgaccccg gcggtgaccg cgccgagctt ggctgccgcg agatacgcca
     1381 cgaggtagtc gggcccgggg ggcagcacga gcgccaggac gtcgccaacc gcgacgccgc
     1441 gggcccggag cccgacggcc acctcgtccg acacccgatc gaggtcttcg aacgagagcg
     1501 cccaaccctc ctcggtcacg taggcgggag cggcaccgaa gcgacgggcg gcttcgcgta
     1561 gggtttcggc gagcatgatc ggctctcgag cgggatcagt tccggcgacg gtggacccaa
     1621 ccagtccggg gcggccgaga gagcgttggg ggaggttggg gtaagtgccc gcatcgtaca
     1681 agtcgtcgcc gcgccgcgag gccgaggcga gcgccgggct ccccgcacag caccgggagc
     1741 tgcgcgcgca ggggcgacgc accttgcggc gcctccttga cgccggactg cgcgtcttcg
     1801 cccgccgcgg ttaccacgcg gcgcgagtcg acgacatcgt gcgagcggcg cgcacctcgc
     1861 acggcacctt ctacctctac ttcgccaaca aggaagacct gctccgcgcg ctggcggtcg
     1921 actgcgcgca cgagctcacc gacctcgccg ccagcatcgg cccgatcggt cgagacgcgt
     1981 cgggacggac cgagctgcgg gccttcctgg aacggttcat cgacacctac cgccgctacg
     2041 gcccggtcat ccgagcctgg atggaggacc aggtggggga ccgtgacgtc gaccgcctcg
     2101 gcgtcaaggc gttcacggcg atcggcggac gcctgggcga acggatgcgc gacacccagg
     2161 tgatgttcga gagcgaggac gcggccgtgg gtgcgctgat ggcgatgctg gagcggctca
     2221 gctacggcgt cgcgtcgggg cggatcaccg ccgacaacga gacgttgctc gacaccctga
     2281 ccacgatcgt ccatcggggg ttcttcgccg cgacggaccc gtgatctcgg gccgctgtca
     2341 cccggcggcg aggatcaccg ttgaagccga cgagaaccag ccgccggtcc cgttcacaca
     2401 gcacagctcg gccccgtcga cctgccgggc gccggcgtcg ccccggagct ggcgggtcgc
     2461 ctccacgagc agaaacatgc cgcgcatccc aggatgacaa gccgacaggc cgccgccgtc
     2521 ggtgttggtc gggaggcttc cgcccacgcg caggcgcccg tcctccacga acccgccgcc
     2581 gtcacccttg gaacagaacc cgagcgcctc gagggtcaac agcaccatgg gcgtgaacgc
     2641 gtcgtagagc tgacagcagt cgatgtcggt cggcgtgacc ccggcccgct cgaacgcggc
     2701 ctgaccggac cggcgtgccg gggagtccgt gaagtcgggc cattcgctca tggtggtgtg
     2761 cgacgtcgcc tcaccggtgc cgagcaccca cactggagga tggcgaaggt cgcgggcccg
     2821 ggcctccgac gtgagaacga ccgcgccgcc gccgtcgctg cggagacagc agtgcagctt
     2881 cgtgagcggg tcggcgatga gacgggatga ctggacgtct tcgatgctga tgggttcccg
     2941 gtggtaggcg tcggggttca gcgaggcgtt gaaccgagtc gagaccgcga tctcggccag
     3001 ctgctcgatg gtcgtgccgt actcgtacat gtggcggcgg gtcgccatcg cgtacttcgc
     3061 gatcaacgcg tggccgtacg gtgcgtcgaa
//
LOCUS       DATCCH010000136         2560 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_471548, whole genome shotgun sequence.
ACCESSION   DATCCH010000136 DATCCH010000000
VERSION     DATCCH010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471548"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1685)
                     /locus_tag="VIJ44_02745"
     CDS             complement(<1..1685)
                     /locus_tag="VIJ44_02745"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEY5164850.1"
                     /translation="MAEPGALAVLDQCVRGLGGDDRPGQRLLTEAVAAAFADSHHLVA
                     QAPTGSGKSLAYLAPAVASGRATVVATATLALQDQLWRKDLPLVAEQSGIPFEAAVLK
                     GRAQYLCLARLRAAVGGEALFDERPGPDLAAELTRLERFAADSETGDASDADVAPGVW
                     RAVSCGPNECPGAARCPAGEDCFAEHARRRADGADILVVNHALYLAHLAAGGTLLPHH
                     EVVIVDEAHALADTATRALGSEVAPGGLRHLAARLRGAGGPAADADAVAEAGERLDDV
                     LGDLDGRVDPTEERLAGVLAAAAERIATAAGHVDQRGDAPRATQTARLAAARLDAVRR
                     VQAPRGDEVAWVEGGERPVLRLAPVDVGSRLAPLLFAHHTCVLLSATLGPGPRFEPFA
                     RRVGLEPTADAAAERGYLAARVESPFDVRGQSLLYVPRALPDPRRDTWADAAGEELCA
                     LVAAAGGRALVLCTSRRAVERFAALLRDRTDHSVLAQGDAANAALLERFAAEETSCLV
                     ATRAFWQGVDVPGPACVLVVIDRVPFTRPDDPLEQARREAVEAAGGSGFRDVD"
     gene            1764..>2560
                     /locus_tag="VIJ44_02750"
     CDS             1764..>2560
                     /locus_tag="VIJ44_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009954240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY5164851.1"
                     /translation="MSDRTVHYRTCPLCEAMCGLEIHVEGDAVPLIRADRDDVWSKGF
                     LCPKGTTLGHLHHDPDRLREPMIRSGDTWRTVGWDEAFARCEEVIRFVLDDVGKAALT
                     AYIGNPTAHNFSLGRYIGLFMGLADFPMIYSAGTVDQWPKNVACALMYGNMWAIPAPD
                     IQRTDYWVVMGANPQASQGSLLACPDVLGEIDRIRDRGGRTVVIDPRRTGTADRADEW
                     IPIVPGTDAAFLLALIHVLFADDLIDLGDVADLVDGVEAVRDACRDFS"
BASE COUNT          373 a          948 c          931 g          308 t
ORIGIN      
        1 aagtcgacat cacggaaccc ggatccgccg gccgcctcga cggcctcccg gcgggcctgc
       61 tccaacgggt cgtcgggacg cgtgaagggg acccggtcga tgacgacgag cacacacgcc
      121 gggcccggga cgtcgacgcc ctgccagaac gcccgggtcg cgacgagaca cgaggtctcc
      181 tcggccgcga accgctccag cagcgccgcg ttcgcggcgt cgccctgggc cagcaccgaa
      241 tggtcggtgc ggtcccgcaa cagggccgcg aaccgctcga cggcccgccg cgacgtgcac
      301 agcaccagcg cccggccacc ggcagcggcg accagcgcgc agagctcctc accggcggcg
      361 tcggcccacg tgtcgcgccg cgggtcgggc aacgcccgcg ggacgtacag cagcgactgg
      421 ccccgaacgt cgaacggcga ctcgacgcgg gccgcgaggt agccgcgctc ggcggccgcg
      481 tctgcggtcg gctccagacc aactcggcga gcgaacggct cgaaccgggg accgggcccc
      541 agcgtcgcgg agaggagcac gcaggtgtgg tgggcgaaga gcagcggagc gagccgcgac
      601 ccgacgtcca ccggcgccag gcgcagcacc ggccgctcac cgccctcgac ccacgccact
      661 tcgtcgccgc gcggcgcttg gacccggcgg accgcgtcga ggcgggccgc ggccagccgg
      721 gcggtctggg tggctcgggg tgcgtcaccg cgctggtcga catgaccggc ggccgtcgcg
      781 atccgctccg ccgccgcggc gaggacaccc gcaagccgct cctcggtcgg gtcgacccgg
      841 ccgtcgagat caccgaggac gtcgtcaagc cgctcgccgg cttccgcgac cgcgtccgcg
      901 tcggccgcgg ggccgcccgc gccccggagc cgcgccgcga ggtggcggag accgccgggc
      961 gcgacctcgc tgccgagcgc acgcgtcgcg gtgtcggcga gggcgtgtgc ctcatcgacg
     1021 atgacgacct catggtgggg cagcaaggtc cctcccgccg cgaggtgagc caggtagagg
     1081 gcgtggttga cgacgaggat gtcggcgccg tcagcccggc ggcgggcgtg ctccgcgaag
     1141 cagtcctcac cggcgggaca acgggccgcg cccgggcact cgttcggccc gcacgacacc
     1201 gcccgccaga cccccggcgc gacgtcggcg tcggaggcgt cgccggtctc agaatccgcg
     1261 gcgaagcgtt cgagccgggt gagctcggcg gcgagatcgg ggccaggccg ctcgtcgaac
     1321 aaggcttcgc cgccgactgc ggcgcgcagc cgcgccaggc acaagtactg ggcgcgtccc
     1381 ttcaacaccg cggcttcgaa ggggatgccg ctctgctcgg cgaccagggg gagatccttg
     1441 cgccacagct ggtcttgcag cgcgagggtc gcggtcgcca ccaccgtcgc ccgtcccgac
     1501 gcgaccgccg gagctaggta ggcgaggctc ttgcccgaac cggtcggggc ctgggcgacg
     1561 aggtgatgcg aatcggcgaa ggcggcggcg acggcctcgg tgagaagccg ctgaccgggg
     1621 cggtcgtcgc cgccgaggcc ccgcacgcac tggtcgagga cggcgagcgc cccgggttcc
     1681 gccacacctc ggaacctacc gggggccgcg cgctgtgacg cggaacggcg tatcgtgctc
     1741 cagccgcttc accgagggaa cgagtgtccg accgcaccgt ccactaccgc acctgcccgc
     1801 tatgtgaggc gatgtgcggc ctggagatcc acgtcgaggg tgacgcggtg ccgctgatcc
     1861 gcgctgaccg cgacgacgtg tggagcaagg gtttcctctg cccgaaaggc accacgctcg
     1921 ggcacctgca ccacgacccc gaccggttgc gggagccgat gatccgcagc ggcgacacgt
     1981 ggcgcaccgt cggctgggac gaggcgttcg cgcgctgcga ggaggtcatc cgcttcgtgc
     2041 tggacgacgt ggggaaggca gccctcaccg cgtacatcgg caacccgacg gcgcacaact
     2101 tctcgctcgg tcgctacatc gggctgttca tggggttggc cgacttcccg atgatctact
     2161 cggcggggac cgtcgaccag tggcccaaga acgtcgcgtg cgcgctcatg tacgggaaca
     2221 tgtgggcgat cccggctcct gacatccaac gcaccgacta ttgggtggtg atgggcgcca
     2281 acccgcaggc gtcccaaggc agcctgctcg cctgccccga cgtgctcggt gagatcgacc
     2341 ggatccgcga ccgtggggga cgcacggtcg tgatcgaccc tcgccggacc ggcaccgcgg
     2401 accgagccga cgagtggatc cccatcgtcc ccggaaccga cgccgcgttc ctcctcgcgc
     2461 tgatccacgt cctgttcgcc gacgacctca tcgacctcgg agacgtcgcc gacctcgtcg
     2521 acggggtcga ggcggtccgc gacgcctgcc gggacttctc
//
LOCUS       DATCCH010000137         4340 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_471566, whole genome shotgun sequence.
ACCESSION   DATCCH010000137 DATCCH010000000
VERSION     DATCCH010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4340
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471566"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1266
                     /locus_tag="VIJ44_02755"
     CDS             <1..1266
                     /locus_tag="VIJ44_02755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG family protein"
                     /protein_id="HEY5164852.1"
                     /translation="DIVAALIGAAAEHGEVTYAVPGSPSVAERTVALLHDAARDGRVE
                     LATVPGLSFAELAWTQLGVDPMTDLARVVDARALESASLDLGGLLLVAQCDDPLLCSD
                     VKLILLERLPPDTPVTVLARLGGPDERVDTVALADLDRVVPDHLTSLAVQLPDPSPGR
                     ELARLLELARLLRRPGGCPWDAEQTHHSVTRYLLEEAYEVVDAVEALPIPAPADTPAD
                     DPAYTRLVDELGDLLYQVIFHAMLAEEAGAFGMGDVARGVHDKLVHRHPHVFGDAAAA
                     TSGDVMRNWEQIKKGERGATSIMDGITPGLPSLLYAHKLLRKAASVGLDPGDVAVSLD
                     RIDAATVALRAGADDEPVLGDLLAGAVALARALGVDAESALRGWSVRFRARFEAMEAD
                     AAGDGVDLATLDPGAAAARWDAAGPTLRS"
     gene            complement(1256..2503)
                     /locus_tag="VIJ44_02760"
     CDS             complement(1256..2503)
                     /locus_tag="VIJ44_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY5164853.1"
                     /translation="MTDVARDEVGTEPEAELGLNPFAPGYFEDPYRQYRQLREQAPVH
                     HNPIGPWTITRYEDCSRLLRDPRMSVMPANADVDPRTEMIERFGYERSERGTRAILSL
                     DPPDHTRIRRLAQQAFTPRRVELLVARIQSLVDGMLDEVEPAGGMDVIADLAFPLPFA
                     VISEMLGMPDTDRAEMRRWSHTLIKSLEPIVMPDEFPAILDAGDHLTEHVTAAIEWKR
                     REPGDDLLSALIAAEEEGDRLSPAELRDQVVLLYLAGHETTVNLIGNGTLAVLRHPDQ
                     HAALRDDPDLIGNAVEELLRYDSPVQFSRRITLADVELDGQTIPERSFVFTCLGAANR
                     DPEHFGPDADDLDLRRRDAPHHISFGGGIHHCLGAVLARTEARAAIGTLVRRFPDLTL
                     GGDDPAWNGRVVLRGLDELPVSF"
     gene            2688..3986
                     /gene="eno"
                     /locus_tag="VIJ44_02765"
     CDS             2688..3986
                     /gene="eno"
                     /locus_tag="VIJ44_02765"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEY5164854.1"
                     /translation="MSEIVEVIGREILDSRGNPTVEAEVQLVSGARGRAAVPSGASTG
                     THEAVELRDGDGRYRGKGVRHAVEHVNTEIADAVTGLDGDDQRLLDDELAVLDGTANK
                     GRLGANAILAVSLAVAHAAADEAGLPLYRYLGGVDAHVLPMPLLNVLNGGAHAASNVD
                     LQEFMLAPVGAASFSEALRWGTETYHALRALLDERGLATGLGDEGGFAPNLPSNEEAI
                     SLLLAAIEAAGYQPGVEVALALDVAATELYADGTYTLAGEDRAFDAAGWVDYLAGLGD
                     RYPIVSIEDGMAEDDWDGWALLTARLGQLQLVADDVFVTNPERLQRGIDAGVANSILI
                     KVNQIGTLTETLDTMLLASRHGYTAVMSHRSGETEDTTIADLAVGTNCAMIKAGAPAR
                     TDRVAKYNQLLRIEEELGPVAAYLGGGALAGGARGHGQRG"
     gene            3970..>4340
                     /locus_tag="VIJ44_02770"
     CDS             3970..>4340
                     /locus_tag="VIJ44_02770"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEY5164855.1"
                     /translation="MGNVAEPRRRRGSAAPPNRTRRRAAPSTAKPASTRRRASQRVRR
                     RRTLLTATLLGLAVVGLLFVFVYPTRTYLQQRNQISAAQHHLAQLNRETARLDGQSRQ
                     LQADAAVERIAREQYGLVKPG"
BASE COUNT          616 a         1654 c         1482 g          588 t
ORIGIN      
        1 gacatcgtcg ccgcgctgat cggcgccgcc gccgagcacg gcgaggtgac ctacgccgtc
       61 ccggggagcc cgagcgtcgc cgaacgcacc gtggctctcc tccacgacgc ggcccgggac
      121 ggacgggtcg agctcgccac ggtccccggc ctgtcgttcg ccgagctggc ctggacccaa
      181 ctcggggtcg atcccatgac cgacctggcc cgcgtcgtcg acgcccgggc cctcgagtcc
      241 gcgtcgctcg acctgggcgg gctcctcctc gtcgcgcagt gcgacgaccc gctgctgtgc
      301 tcggacgtga agctgatcct gctggaacgg ctcccgcccg acacaccggt cacggtcctc
      361 gcccggctcg gtggccccga cgaacgcgtc gacaccgtcg ccctcgccga cctcgaccgt
      421 gtcgtccccg accatctcac ctccctggcg gtgcagcttc ccgacccgtc accgggacgc
      481 gagctcgccc gcctcctcga gctggcgcgg ctgctgcgcc gccccggcgg ctgcccgtgg
      541 gacgccgagc agacccacca ctcggtgacc cgctacctcc tcgaagaggc ctacgaggtc
      601 gtcgacgccg tcgaggcgct cccgatcccc gcgcccgccg acacgccggc cgacgacccg
      661 gcgtacaccc gtctcgtcga cgagctcggt gaccttctct atcaggtgat cttccacgcc
      721 atgctcgccg aagaagccgg agcgttcggg atgggtgacg tcgcgcgcgg cgtccacgac
      781 aagctcgtgc accgccaccc gcacgtcttc ggcgatgccg ccgccgcgac cagcggcgac
      841 gtgatgcgca actgggagca gatcaagaag ggcgagcggg gcgcgacgtc gatcatggac
      901 ggcatcaccc ccgggctccc gtcgttgctc tacgcccaca agctcctccg caaggccgcg
      961 tcggtcggcc tcgaccccgg ggacgtcgcc gtgtccctcg atcggatcga cgccgcgacc
     1021 gtggcgttgc gcgccggcgc cgacgacgag ccggtcctcg gagatctcct cgccggggcg
     1081 gtggcgctcg cccgggccct cggcgtcgac gccgaatcgg cgctgcgagg ctggtcggtg
     1141 cggttccgag cccggttcga ggccatggaa gccgacgccg ccggcgacgg cgtcgatctc
     1201 gccaccctcg atcccggcgc cgcggcggcc cgctgggacg cggcgggccc gacgctcaga
     1261 agctgaccgg cagctcgtcg aggccgcgta gcacgacccg gccgttccag gccggatcgt
     1321 cgccgccgag cgtcaggtcg gggaagcgtc gcaccagcgt cccgatcgcg gcgcgcgcct
     1381 cggtgcgggc gagcaccgcg ccgagacagt ggtggatccc accgccgaac gagatgtggt
     1441 ggggtgcgtc gcggcggcgc aggtccaggt cgtcggcgtc gggaccgaaa tgctcgggat
     1501 cgcggttcgc ggcgccgagg caggtgaaca cgaacgatcg ctccgggatg gtctggccgt
     1561 cgagctcgac gtcggcgagc gtgatgcgac gcgagaactg caccgggctg tcgtagcgca
     1621 gcagctcctc gaccgcgttg ccgatgagat ccgggtcgtc gcgcagcgcc gcgtgctgat
     1681 cggggtggcg cagcacggcg agggtcccgt tgccgatcag gttcaccgtt gtctcgtggc
     1741 ccgcgaggta gaggagcacc acctggtccc gcagctccgc cggggacagc cgatcccctt
     1801 cttcctcggc ggcgatcaac gcgctgagca gatcgtctcc cggttcgcgc cgtttccact
     1861 cgatcgccgc ggtgacatgc tcggtcaagt ggtcaccggc gtcgaggatg gcggggaact
     1921 cgtcgggcat cacgatcggc tcgagggact tgatcaacgt gtgtgaccag cggcgcatct
     1981 ccgcccggtc ggtgtcgggc atgccgagca tctccgagat caccgcgaac ggcagcggga
     2041 acgccaagtc ggcgatcaca tccatcccac cggcgggctc gacctcgtcg agcatgccgt
     2101 ccaccagcga ctggatccgc gccacgagca gctccacccg ccggggtgtg aacgcctgct
     2161 gagcgaggcg gcggatccgg gtgtggtcag gcgggtcgag gctgaggatc gcccgggtgc
     2221 cgcgctcgct gcgctcgtag ccgaaccgct cgatcatctc ggtgcgggga tccacgtcgg
     2281 cgttggctgg catcaccgac atgcgggggt cgcgcaggag ccgggagcag tcctcgtagc
     2341 gggtgatcgt ccacggcccg atcgggttgt ggtgcaccgg ggcctgctcg cgcagctggc
     2401 ggtactggcg gtaggggtcc tcgaagtagc cgggggcgaa cgggttcagc cccaactcgg
     2461 cttccggctc ggttcccacc tcgtcgcggg ccacgtcggt cacgaccacg agtgtatgga
     2521 cccacctccc ccgcctggct cggcccaacg cccccaggcc ctgtcgggcc gccccgccgg
     2581 gcccggattg ggagcccttc ccagaccggc cccggacggc tacattcgtt cctcctgtca
     2641 cacaaaccac acgggccaca gccggcgagc ccccgaggtg tcgggccatg agcgagatcg
     2701 tcgaggtcat cggccgcgag atcctcgatt cgcggggcaa tccgacggtc gaagccgagg
     2761 tgcaactggt ctccggggcc cgaggccggg cggcggtccc gagcggggcc agcaccggca
     2821 cccacgaggc cgtcgagctc cgcgacggtg acggccgcta ccgcggcaag ggcgtgcgcc
     2881 acgccgtcga gcatgtgaac accgagatcg ccgacgcggt gacgggtctc gacggcgacg
     2941 accagcgcct cctcgacgac gagctcgcgg tcctcgacgg gaccgcgaac aagggccgcc
     3001 tcggcgcgaa cgccatcctc gcggtgtccc tcgcggtggc tcacgccgcc gccgacgagg
     3061 ccggcctgcc cctgtaccgg tacctcggcg gggtcgacgc ccacgtcctg ccgatgccgc
     3121 tcctcaacgt cttgaacggc ggcgcccacg ccgccagcaa cgtcgacctc caggagttca
     3181 tgctcgcccc ggtcggcgcc gccagcttca gtgaggcgct ccgttggggc accgagacgt
     3241 accacgcgct ccgcgccctc ctcgacgagc ggggtctcgc caccgggctc ggcgacgagg
     3301 gcggcttcgc gccgaacctg ccctcgaacg aagaagccat ctcgctgctc ctcgcggcga
     3361 tcgaagcggc cggctaccaa ccaggcgtcg aagtcgcgct ggccctcgac gtcgccgcca
     3421 ccgagctcta cgccgacggg acctacaccc tcgccggcga agaccgcgcc ttcgacgccg
     3481 ccggctgggt cgactacctc gccgggctcg gtgaccgtta cccgatcgtg tccatcgagg
     3541 acggcatggc cgaagacgac tgggacgggt gggctctgct caccgcccgg ctcggccaac
     3601 tccaactcgt cgccgacgac gtgttcgtca ccaaccccga acggctccag cggggcatcg
     3661 acgccggggt cgcgaactcg atcctgatca aggtcaacca gatcggcacg ctgaccgaga
     3721 ccctcgacac catgctgctc gcgtctcggc acggctacac cgcggtgatg tcgcaccgca
     3781 gcggcgagac cgaagacacc accatcgccg acctcgccgt cgggaccaac tgcgccatga
     3841 tcaaagccgg agcgccggcc cgcaccgacc gggtcgcgaa gtacaaccag ctcctccgca
     3901 tcgaggagga gctcggaccg gttgccgcgt acctcggtgg cggcgcgctc gcgggcggag
     3961 ctcgggggca tgggcaacgt ggctgagccg cgccgcaggc gcggctcggc cgcgcccccg
     4021 aaccgcaccc gccgtcgggc cgcgccgtcg accgcgaagc cggcatcgac tcgccggcgc
     4081 gcctcgcagc gggtccgccg gcgccgcacg ctgctcaccg cgacgctcct cggcctggcg
     4141 gtcgtcggct tgctcttcgt cttcgtctac ccgacccgca cctacctcca gcagcgcaac
     4201 cagatcagcg ccgcgcagca ccacctcgcg cagctcaacc gcgagaccgc ccgcctcgac
     4261 ggccagtccc gccagctcca ggccgacgcc gcggtcgagc gcatcgcccg cgagcagtac
     4321 gggctggtca agccgggtga
//
LOCUS       DATCCH010000138         8920 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_180959, whole genome shotgun sequence.
ACCESSION   DATCCH010000138 DATCCH010000000
VERSION     DATCCH010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 8920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8920
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180959"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..425)
                     /locus_tag="VIJ44_02775"
     CDS             complement(45..425)
                     /locus_tag="VIJ44_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164856.1"
                     /translation="MSVARNVAQGCGCAPDETIRWLVVSLLGLAGTATLATRWWLQHQ
                     ARRTATAPTEVPASVSDPRSRAERCAPVVAGLVIFGLALLVAVVPMSSDPALLVLALG
                     ATVGTAGFGLGSRRTRRDSQTAPQ"
     gene            complement(505..1539)
                     /locus_tag="VIJ44_02780"
     CDS             complement(505..1539)
                     /locus_tag="VIJ44_02780"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEY5164857.1"
                     /translation="MLYPTEWYGDREAFAAEIDLLGRLDPQVQVLVEPYVEPHELRSA
                     RGKPGAEQLRSAAPALTDEHRTAFGQLDVALAIDLPFDVSSVAPRLRWVQAVGAGTGQ
                     LQSAGLAAAGIRLTTAAGANAVAIAEFALARVLQHWKRFRELDDAQQRHDWDPLYGQQ
                     LAGRTLGLIGLGAINSAVAARAQAFDLRVVATRRSATPGSTAPDVDALYPPDELAVML
                     GQCDAVIAAVPETPETTGLMDRDAFAAMKPGAFFCNVGRGSLVDEAALVDALRAGHLR
                     AAALDVASVEPLPAEDPLWDAPNLYLSSHSASSPAALFVNLHLLFRENLSRFLRGDAL
                     ENEVDMSRGY"
     gene            complement(1625..4039)
                     /locus_tag="VIJ44_02785"
     CDS             complement(1625..4039)
                     /locus_tag="VIJ44_02785"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY5164858.1"
                     /translation="MPGALDGLRVLDLTWGAAGALGVLLVAEQGADTIKVEPPGGDPF
                     RGYDGYKVWTRSRRAVQVDLKSDLGRAAFLQLAASADVVVESFRPGVADRLGVGYDAC
                     AAINPRLVYCSVPGWPDGHRNADRPGYDALIQAASGQQWEQPGWRPGPIFLAMPMPSM
                     GAIYLVASGVLAALIARESTGRGQHVRTSLLQGVFLYTTQIWQQAERADAAYYGLMAK
                     THPPGIHQGMLFECANREFAHVSIMSGLTPLKSLDEVLGVDTPPPSALEGLLPLEQQL
                     LMNERRREAFLRWPRDKLVAELLANNHAVEAVVEPEDQFAHPQLQANGMVAVVDDPDL
                     GPVTQIGVPIHLLGTPGAIAGPQPGVGQHDGEVWGDLGYDTDEITAITGGGKRLVIGT
                     GARRAESRQAPAIPPAPPPDVTVRRALDGVRVIDFGQYLAGPFGPMILADLGADVIKV
                     EPIAGDGMRMAATPFFGCQRGKRDLALNIKDPEGLELALALVRGADVVHHNMTKGTAA
                     RLGLDYDACRAVNPAVVYCNTYAYGLDGPLSHFGGLDPLYQASAGLEHEAGAVGHGHE
                     PLYYRYGMCDASNAMLSVVGVLAALVHQRRTGQGQELWTSLMDGGAVFASDVLLDRDG
                     KPTWRPKLDARQQGFGPFYRLYETNDGWIQVCAISDAHRTALLSGTGVDRGVDAGSTE
                     AALENVFATKSAVTWSHVLDDAGVPNEVAVDVDGGKLALFDADAERLGLVTEYEHPLM
                     GRLRQFGETISFSDTPGRIFGPPPRVGEHTRALLEELGVPSGDHERLRAQGIVGWPDD
                     TYAWGW"
     gene            4203..4598
                     /locus_tag="VIJ44_02790"
     CDS             4203..4598
                     /locus_tag="VIJ44_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164859.1"
                     /translation="MVAQPRSRADVASRLAHRLGIEFHSDLLEIVPPVAWDEFPTRDE
                     LGEALRARDEHLDRRLRAIRDALAAAHHDQARQLVGRALALALAGVVAASAALVRSRH
                     GSGSVTGRARDHGGSCAGQREALHRRRVG"
     gene            4555..6000
                     /locus_tag="VIJ44_02795"
     CDS             4555..6000
                     /locus_tag="VIJ44_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018657767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEY5164860.1"
                     /translation="MQVNEKLYIGGEWVEPETSATIDVVSPHTEKVIGRVPDASPADM
                     DRAVTAARQAFDDGPWPRMTPAERAEGIKRLSGAVQSRAQDLADTISSENGSPKSWSL
                     MGQVFSATMVLDTYAGLASGYGWEERRTGALGAPVLVRRAPVGVAAGIIPWNVPLFIS
                     ALKLGPAMAAGCPIVLKPSPETPLDGYLLADAVAEADLPPGVVNIVAAGRETGEHLVR
                     HPGIDKVSFTGSTAVGKHIGQICGGMLKRLTLELGGKSAAIVLDDVNLDEATLGNLVQ
                     SGLMNNGQVCGAQSRVLVSKRRYQDVVDALGAAVGALKVGDPLDESVQVGPLVAERQR
                     TRVEGFLDAGKEAGARVVVGGGRPSDQPTGWYIEPTVFADVNNDMKIAREEIFGPVLS
                     VIPYNDEREAIALANDSDYGLCGSVWTADPEHGAAVAAQVRTGVVAVNSSMILDFNAP
                     FGGFKQSGIGRELGPEGIAPYTEYQTIILPA"
     gene            complement(6029..6979)
                     /locus_tag="VIJ44_02800"
     CDS             complement(6029..6979)
                     /locus_tag="VIJ44_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY5164861.1"
                     /translation="MATRTRKHSAEQQDAVGAYLESIGRHDLLTKDDEARLGRLVMDG
                     LAALERLESGGRLTPTQRRDAHQAIAAGQRARDEFVVANLRLVVSIARRYQSTGLPFG
                     DLIQLGNLGLLHAVEKFDFRKGFKFSTYATWWIRQAITRGYADTARVIRLPAHAEDTY
                     RLVARTSDDLEARLGRQATDDEVAHASGVDPSRVGDVRRFPTRMASVDAPIGDGDSDD
                     LLLGDTIADEDSMFADEIADRDARTHVVQTMLDILDDRERRIVELRYGLDGGGPRDLA
                     EVGKEIGVTRERVRQIEARVLSKLRHPSMAGVDAGDALAS"
     gene            complement(7056..7790)
                     /locus_tag="VIJ44_02805"
     CDS             complement(7056..7790)
                     /locus_tag="VIJ44_02805"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002696019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEY5164862.1"
                     /translation="MTLPAEPASPVGSFELRAGDARELLLALPDDSVDLICTDPPYNL
                     APYSRGNIEMSWRSDFNNDLADWDRIAFDPGDWLDELRRVLAPHGNLFAFTSYNLLGR
                     WHEVFDPVFDTFQFLVWHKTNPAPKLRRAGFLNSCELVVCCWDRGHTWNFGRQRDMHN
                     FIEAPICAGHERVKDPKHPAQKPLAVVRRLVEMASRPGDLVLDPFMGVGTTGVAAVEL
                     GRRFLGFELDPGYASAAQARLEALDQ"
     gene            7910..>8920
                     /gene="xseA"
                     /locus_tag="VIJ44_02810"
     CDS             7910..>8920
                     /gene="xseA"
                     /locus_tag="VIJ44_02810"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEY5164863.1"
                     /translation="MTEQPSLLDAESSAHPTFSVAELNQTISDALGETFPTPVWVRGE
                     VQALHVSRNQHTYFELVEKHERRDQVRAAIRVALFRDDRPGVNRALRDAGLRLSDGVE
                     VRIRARVDFWPPAGRLQLVMTAIDPTFTVGRLAADRDRILRVLAAEGVLRRNAALSLS
                     VVPLRIGLVTSGGSAAYRDFVHELEATGYAFRVAHCDVRVQGAAASRRLVWALRRLAQ
                     EELDVIVVVRGGGARSDLAPFDTEIVARAITEMPVPVLTGIGHETDRTIADDVAHTSV
                     KTPTAAAALLVELVDAYVERLAYVTHRVSLRARGVCGVARREVSGVARRLTRVVPASL
                     DRE"
BASE COUNT         1325 a         3084 c         3173 g         1338 t
ORIGIN      
        1 gtccgactgg ggtgccgcga agaagaaggg aaccgccacg ttcgtcactg tggcgcggtc
       61 tgcgagtcgc ggcgcgtccg tctcgatccg agcccgaacc ctgccgtccc gacggtcgcg
      121 ccgagcgcca ggaccagcag cgcgggatcc gagctcatcg ggacaacggc cacgagcaac
      181 gccaacccga agatgacgag acccgccacg acgggcgcgc atcgctccgc tcggctccgc
      241 gggtccgaca ccgaagccgg gacctcggtc ggcgcggtcg ccgtgcgacg cgcctgatgc
      301 tgcagccacc atcgcgtcgc aagggtcgcg gtccctgcga gcccgagcag gctgaccacg
      361 agccagcgga tcgtctcgtc gggagcgcat ccgcacccct gcgccacgtt ccgcgccacg
      421 ctcacccggg ctcccccgtg ctgggcttcg tcggtcggga cgatagtggc ggagccggca
      481 cgcctcgacg cccggagccg gcgttcagta gccccgactc atgtcgacct cgttctcgag
      541 ggcgtcgccg cggaggaacc ggctgaggtt ctcgcgaaac agcaggtgca agttgacgaa
      601 caatgccgcg ggggacgacg cggaatgcga cgacaggtag aggttgggcg cgtcccagag
      661 gggatcctca gcggggagcg gttcgacgct cgcgacgtcc aatgcggccg cgcggagatg
      721 gccggcgcgg agcgcgtcga cgagcgcagc ctcgtcgacg agggatcctc gtccgacgtt
      781 gcagaagaac gcgcctggct tcatcgccgc gaacgcgtcg cgatccatga gcccggtcgt
      841 ctcgggggtc tcgggaaccg ccgcgatgac cgcgtcgcac tgcccgagca tcactgccag
      901 ctcgtcgggc gggtacaggg cgtcgacgtc cggagcggtg gagccgggcg tcgcggaccg
      961 tcgggtcgcc acgacacgga gatcgaacgc ttgcgcccgg gccgcgacgg cactgttgat
     1021 cgcacccaac ccgatgagac ccaacgtgcg gccggccagc tgctgaccat agagcgggtc
     1081 ccagtcgtgg cgttgttgcg cgtcgtcgag ctcccggaac cgtttccagt gctggaggac
     1141 cctcgccaac gcgaactcgg cgatcgcgac cgcgttcgca ccggcggcgg tcgtgaggcg
     1201 gatccccgcc gccgcgaggc ccgcggattg cagctgtccg gtgccggctc cgaccgcttg
     1261 gacccatcga agccgcggcg cgaccgagga gacgtcgaag ggcagatcga tcgccaacgc
     1321 cacgtccagc tggccgaagg cggtgcggtg ctcatcggtc agtgccggag cggcggaccg
     1381 cagctgctcc gcgccgggct tgccgcgggc gctgcggagc tcgtgcggct cgacgtacgg
     1441 ctcgacgagg acctggacct gcgggtcgag gcgaccgagg aggtcgatct cggccgcgaa
     1501 cgcctcgcgg tccccgtacc actcggtggg gtacagcacg ccgaccacca ccgacgaccc
     1561 gtctgggcgg gaaggaatca cgtcgaacct ccggtcaagc agacggcggt cgggtcggtc
     1621 ggggtcacca gccccaggcg taggtgtcgt cgggccagcc gacgatgcct tgggctcgca
     1681 gccgttcatg gtcacccgac ggaactccga gttcctcgag gagtgcccgg gtgtgctccc
     1741 cgacccgtgg gggcggtccg aagatccgtc cgggggtgtc ggagaagctg atcgtctcac
     1801 cgaactgtcg gagccgaccc atgagcggat gctcgtattc ggtcaccaaa ccgaggcgtt
     1861 cggcgtcggc gtcgaacagc gccagcttgc caccgtcgac gtcgaccgcg acctcgttgg
     1921 ggaccccggc gtcatcgagg acatgggacc atgtcaccgc gctcttcgtg gcgaacacgt
     1981 tttcgagggc ggcttcggtc gagcccgcat cgaccccacg atcgacgcca gtgccagaga
     2041 gcagcgcggt tcggtgggcg tcggagatgg cgcacacctg gatccaaccg tcgttggtct
     2101 cgtacagacg gtagaacggc ccgaatccct gctggcgggc gtcgagcttg ggacgccagg
     2161 tcggcttccc gtcccggtcc aacaggacgt cggacgcgaa gaccgccccg ccgtccatga
     2221 ggctcgtcca gagctcctgg ccctggccgg ttcgtcgttg gtggacgagc gcggcgagga
     2281 cccccacgac ggagagcatg gcgttgctcg cgtcgcacat cccgtaccgg tagtagaggg
     2341 gctcgtggcc gtgccctacc gcgccggcct cgtgttccaa cccggcggaa gcctgataca
     2401 gcgggtcgag gccgccgaaa tgcgagagcg gtccgtccag cccgtacgcg tacgtgttgc
     2461 agtacacgac cgccgggttc acggcgcggc acgcgtcgta gtccaagccg agccgcgccg
     2521 cggtgccttt cgtcatgttg tgatgaacga cgtcggcgcc gcgtaccagg gccagggcca
     2581 gctcgagacc ctcggggtcc ttgatgttga gcgcgaggtc gcgcttgccg cgctgacagc
     2641 cgaagaacgg cgtcgcggcc atgcgcatcc cgtcccccgc gatcggctcg accttgatga
     2701 cgtcggcgcc cagatcggcg aggatcatcg ggccgaacgg tcccgcgagg tactggccaa
     2761 agtcgatgac gcgcaccccg tcgagggccc gacggaccgt cacgtccggt ggtggagcgg
     2821 gcgggatcgc cggcgcctga cgcgattcgg cgcggcgggc cccggtgccg atgaccagcc
     2881 gtttccctcc gccggtgatc gcggtgatct cgtcggtgtc gtagccgagg tcgccccaca
     2941 cctcgccgtc gtgttgaccg acgcccggct gggggccggc gatcgcgccc ggcgtgccga
     3001 gcaggtggat cgggacgccg atctgggtca ccgggccgag atcggggtcg tcgacgaccg
     3061 ccaccatccc attggcctgc agctggggat gcgcgaactg gtcttcgggc tccacgaccg
     3121 cttcgaccgc atggttgttc gcgagcagct cggcgaccag cttgtcgcgc ggccagcgga
     3181 ggaacgcttc ccgacgccgc tcgttcatca ggagctgctg ttcgagtggc agcagacctt
     3241 cgagcgccga cggtggcggg gtgtccacgc ccaggacctc atccaggctc ttgagcggtg
     3301 tcaacccgct catgatcgag acgtgggcga actcgcggtt cgcgcactcg aagagcatcc
     3361 cttggtggat tcccggcgga tgcgtcttgg ccatcaggcc gtaataggcg gcgtcggcgc
     3421 gctcggcttg ctgccagatc tgggtcgtgt acaggaacac gccttgcagg aggctggtgc
     3481 ggacgtgctg gccccgcccc gtcgactcgc gtgcgatcag cgccgccagc acaccgctcg
     3541 ccaccaggta gatggccccc atgctcggca tcggcatggc gaggaagatg ggacctggcc
     3601 gccaccccgg ctgctcccac tgctgacccg aggcggcttg gatcagcgcg tcgtaccccg
     3661 gccggtcagc gttgcggtga ccgtcgggcc accccggcac cgagcagtag accagacgag
     3721 ggttgatcgc cgcgcaggcg tcgtagccga cgccgagccg gtcggcgacc ccggggcgga
     3781 acgactcgac gaccacgtca gctgacgccg cgagctgtag gaacgcggcc cggccgaggt
     3841 ccgacttcag gtcgacctgc accgcacgcc gcgaccgggt ccagaccttg tagccgtcgt
     3901 agccccggaa cgggtcaccg cccggcggct cgaccttgat ggtgtccgcg ccctgctccg
     3961 cgacgaggag gacaccgagc gcgcccgctg cgccccacgt caggtcgagg acacgcagac
     4021 catcgagcgc ccccggcatg ctcgcatcct tccatggacc tgacatcgac gtgagctgag
     4081 gcgccgcacc ggcgcggcac cgcgtcgggg tgcgcgatgt cacaccgggg cgcgacggtc
     4141 gcggggcctc gggagccggg cgtcgcggca cccccgcccg tcctcacgaa tcggaggcca
     4201 cgatggtcgc ccaaccccgc tcccgcgccg acgtcgcgag tcgcctcgcc catcgtctcg
     4261 gcatcgagtt ccactccgat ctgctcgaga tcgtgccgcc ggtcgcgtgg gacgagttcc
     4321 ccacccgcga cgagctcggc gaggcgctcc gcgcccggga cgagcacctc gaccggcgcc
     4381 tccgggccat ccgcgacgcc ctcgccgccg cgcaccacga ccaggcccga cagctcgtcg
     4441 gacgggccct cgccctcgcc ctggccggcg tcgtcgcggc gtcggccgcc ctggtccggt
     4501 cccggcacgg aagcggtagc gtcaccgggc gcgcccgcga ccacggaggc agctgtgcag
     4561 gtcaacgaga agctctacat cggcggcgag tgggttgaac ccgagacgtc ggccaccatc
     4621 gacgtcgtct ctccccacac cgagaaagtc attggacggg tccccgacgc gtcacccgcc
     4681 gacatggacc gtgccgtcac cgcggcccgc caggcgttcg acgacgggcc ctggccgcgg
     4741 atgaccccgg cggaacgagc cgagggcatc aagcgcctct caggggcggt ccagtcgcga
     4801 gcccaggacc tggccgacac catctcgtcc gagaacgggt cacccaagtc gtggtcgctg
     4861 atgggccagg tcttctccgc gacgatggtc ctcgacacgt acgccggcct cgcctcgggg
     4921 tacgggtggg aagagcggcg caccggcgcg ctcggcgccc cggtgctcgt ccgtcgagcg
     4981 ccggtcggtg tcgccgcagg aatcatcccg tggaacgttc cgctgttcat cagcgccttg
     5041 aagctcggtc cggccatggc cgccggctgc cctatcgtgc tgaagccgtc cccggagacg
     5101 ccactcgacg ggtacctgct cgccgacgcc gtcgccgagg ctgaccttcc gccgggggtc
     5161 gtgaacatcg tcgccgccgg ccgcgagacc ggcgagcacc tcgtccgcca cccgggtatc
     5221 gacaaagtct cgttcaccgg gtccaccgcg gtcggcaagc acatcggtca aatctgcggc
     5281 gggatgctga agcgcttgac cctcgagctc ggcggcaaat ctgcggcgat cgtgctcgac
     5341 gacgtcaacc tcgacgaggc gacgctcggc aacctcgtcc agtccgggct catgaacaac
     5401 gggcaggtct gcggcgcgca gagccgcgtc ctcgtctcga agcgtcgcta ccaagacgtc
     5461 gtcgacgcgc tcggcgccgc cgtcggcgcc ttgaaggtcg gtgacccgct cgacgagtcg
     5521 gtccaggtcg gcccgctcgt cgccgaacgc cagcggaccc gcgtcgaagg gttcctggat
     5581 gccgggaaag aggccggagc tcgagtcgtg gtgggcggag gacggcccag tgaccaaccg
     5641 accggctggt acatcgagcc gacggtgttc gccgacgtga acaacgacat gaagatcgcc
     5701 cgcgaggaga tcttcggacc ggtcctctcc gtcatcccct acaacgacga gcgcgaggcg
     5761 atcgccctcg ccaacgactc cgactacggg ctgtgcggat cggtgtggac cgccgacccc
     5821 gagcacgggg ccgccgtcgc ggcccaggtt cggaccggtg tcgtcgcggt caactcgtcg
     5881 atgatcctgg acttcaacgc cccgtttggt gggttcaagc agtcagggat cggtcgcgag
     5941 ctcggccccg aaggcatcgc cccgtacacc gagtaccaga cgatcatcct gccggcgtag
     6001 gccggcaggt cacccgaggc gggcgggttt agctggcgag cgcgtcgccg gcgtcgacgc
     6061 ccgccatcga cgggtggcgc agcttcgaga ggacgcgggc ttcgatctgg cggacccgct
     6121 cgcgggtgac gccgatctcc ttgccgacct cggcgaggtc gcgggggccg ccgccgtcga
     6181 gcccgtaccg aagctccacg atgcggcgct cccggtcgtc gaggatgtcg agcatcgtct
     6241 ggaccacatg ggtgcgggcg tcacggtcgg cgatctcgtc ggcgaacatc gagtcctcgt
     6301 cggcgatggt gtcgccgagg agcaggtcgt cgctgtcgcc gtctccgatg ggggcgtcga
     6361 cactggccat gcgggtcggg aaacgccgga catcaccgac gcgggacggg tcgacaccgc
     6421 tggcgtgggc gacctcgtcg tcggtggctt ggcggcccag tcgcgcctcg aggtcgtcag
     6481 aggtgcgggc aacgagccgg taggtgtcct cggcgtgcgc cgggagtcgg atgacgcgcg
     6541 cggtgtcggc gtacccgcgt gtgatcgcct ggcggatcca ccaggtcgcg taggtcgaga
     6601 acttgaagcc cttccggaaa tcgaacttct cgaccgcgtg gaggagaccg aggtttccga
     6661 gctggatcag gtcgccgaac ggcaagccgg tgctctgata ccggcgggcg atcgagacca
     6721 ccaggcggag gttggcgacg acgaactcgt cacgggcgcg ctgtccggcg gcgatcgcct
     6781 ggtgggcgtc gcgtcgctgg gtcggggtca ggcgcccgcc cgattcgagg cgctcgaggg
     6841 ccgcgaggcc gtccatgacg aggcggccga ggcgcgcctc gtcgtctttg gtcagcaggt
     6901 cgtggcggcc gatcgactcc aggtaggcgc cgacggcgtc ctgctgctca gcgctgtgtt
     6961 tgcgggtccg cgtagccatt gtctgatctc ccgggggttc gtagtcatca acgtccgcgg
     7021 atgcctcaga cgttcccatg tgacgtcagg cacaatcact ggtccagggc ctccaggcgg
     7081 gcttgggcgg cggaggcgta cccggggtcg agctcgaagc cgaggaatcg gcgtccgagc
     7141 tccacggcgg cgaccccggt cgtcccgacc cccatgaacg ggtccagcac caggtcaccg
     7201 ggccggctgg ccatctccac cagccgccgg accaccgcga ggggcttttg ggccgggtgc
     7261 ttcgggtctt tgacccgctc gtggccggcg cagatcgggg cctcgatgaa gttgtgcatg
     7321 tcccgctggc gtccgaagtt ccaggtgtgg ccccgatccc agcagcacac gaccagctcg
     7381 caggagttga ggaacccggc ccgccgcagc tttggggcgg ggttcgtctt gtgccagacg
     7441 aggaactgga aggtgtcgaa gaccgggtcg aatacctcgt gccatcggcc caggaggttg
     7501 taggacgtga acgcgaagag gttcccgtgg ggggccagga cccggcgcag ctcgtcgagc
     7561 cagtcgccgg ggtcgaaggc gatccggtcc cagtcggcga gatcgttgtt gaaatcggag
     7621 cgccagctca tctcgatgtt gccccgcgag tagggcgcga ggttgtacgg cgggtcggtg
     7681 cagatcaggt cgaccgaatc gtcggggagg gcgagcagca gctcgcgggc gtcaccggct
     7741 cgcaattcga aggatccaac cggcgaggcc ggctcggcag ggagggtcac cgggaccatc
     7801 gaagcagatg gcctcggacc gtccgcgttc ttcgccgctg gtccctgggg gcctcggtcc
     7861 tggttcgagg gcaatactgg ctcggtgcgt cgattcccgg tgcctgctgg tgactgagca
     7921 gccgtccttg ctcgatgccg aatcgagcgc ccacccaacg ttcagcgtcg ctgagctcaa
     7981 ccagacgatc agcgacgcgc ttggcgagac gttcccgacg ccggtgtggg tacggggcga
     8041 ggtgcaagcg ctgcacgtgt cgcgcaacca gcacacctac tttgagctcg tggagaagca
     8101 tgagcggcga gatcaggtcc gcgccgcgat ccgggtcgcg ctcttccgcg acgaccgccc
     8161 cggtgtgaac cgagcgttgc gcgacgccgg gctccgcctc tccgacgggg tggaggtgcg
     8221 aatccgcgcc cgggtggact tctggccgcc ggcgggccgg ctgcagctgg tcatgaccgc
     8281 gatcgacccg accttcaccg tcgggcgcct cgccgccgac cgggaccgga tcctgcgcgt
     8341 cctggccgcc gaaggtgtcc tgcgtcgcaa cgcggcgttg agcttgtcgg tggttccgct
     8401 acggatcggg ctcgtgacga gcggcgggag cgcggcgtac cgagacttcg tccacgagct
     8461 cgaggccacc gggtacgcgt ttcgtgtcgc gcactgcgac gtccgcgtgc aaggcgcggc
     8521 agcttcgcgc cgactggtgt gggcgctgcg gcgcctggcg caagaggagc tcgacgtcat
     8581 cgtggtcgtg cgaggtggcg gtgcccggag cgacctggca ccgttcgaca ccgagatcgt
     8641 cgcccgagcc atcaccgaga tgcccgtccc ggtcctgact gggatcggtc acgagaccga
     8701 ccgcaccatt gctgacgacg tcgcccacac cagcgtcaag actccaactg cggccgccgc
     8761 gctcctcgtc gagctcgttg acgcctacgt ggagcgattg gcgtacgtga cacatcgcgt
     8821 ctcgttgcgg gcccgcggcg tgtgcggtgt cgcgcgacgt gaggtgtctg gtgtggctcg
     8881 tcgtctcacc cgcgtggtgc cggcgtcgct cgaccgcgag
//
LOCUS       DATCCH010000139         3440 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_181106, whole genome shotgun sequence.
ACCESSION   DATCCH010000139 DATCCH010000000
VERSION     DATCCH010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181106"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /locus_tag="VIJ44_02815"
     CDS             <1..904
                     /locus_tag="VIJ44_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164864.1"
                     /translation="ITLILFGLGAITYARHPEGIVEFQTRRSILEMARGRALGARAKA
                     LAAAKQLPTAYTPVSRVILGCAAGPGLYLAYLLVRSATGLHAHFTGTAPYLRVQGQWA
                     TAHSTTLLCFILPSVAFLLAWMWRTDTVLRRQQGDRRGKLVIIGGAGLGALLGFLLQS
                     TGDLPGSALDCALVGIPVGVAVGVFPWLPHQVELVARAKGWLEAPITWKVGRTPAVFV
                     LTGVYLFSRLTTSTPPTGWPVCLVATVLTIVSLQSVGGIQGAVNELAIGNEGEARRRA
                     EGLGQPLVGAPPAATVAAPVGSAE"
     gene            901..1704
                     /locus_tag="VIJ44_02820"
     CDS             901..1704
                     /locus_tag="VIJ44_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5164865.1"
                     /translation="MSETTARTSERSVRPGALLDAAGITKRFAGITALNGVNLDVQPG
                     EMVGLIGPNGAGKTTFFNCLLGMLRPEGGTVHFDGRDITRLPVHRRARLGFGRTFQRL
                     ELFSGMTVREHFLVTERARRGTGRLWKDLLNLAKPSVEEQALADRMIEIVGLQAEADE
                     PVEALSLGRGRLVELGRALMTQPKLLLLDEPSSGLDVRETASLADKLAAVQTQHEFAV
                     LLVEHDVEMVSNLVTRLYVLDFGELIAFGPTQSVLGDEAVRKAYLGDVV"
     gene            1701..2465
                     /locus_tag="VIJ44_02825"
     CDS             1701..2465
                     /locus_tag="VIJ44_02825"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5164866.1"
                     /translation="MTNDLAAEKPLLELRNIEASYGPFRSLFGVSFAIQERGVTALLG
                     SNGSGKTTVVRICSGLLHPTGGQLLYDGNDVSQMRAYKLARLGVVHAPEGRSVFGTLS
                     VEENLALTFRQVFGRRGCREAIDRSYELFPRLGERRRQIAGTLSGGEQRMLSLARVLV
                     RGPKLLITDELSLGLAPVIIDEVYATLATIRASGTALLIVEQHIRQALTLADHVVVMA
                     KGRVSLSGRTDELGDIAERILPAVHGEAGSSNGAWS"
     gene            complement(2541..3056)
                     /locus_tag="VIJ44_02830"
     CDS             complement(2541..3056)
                     /locus_tag="VIJ44_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164867.1"
                     /translation="MRIRAVTLLTALVTVGMLVVGASSAEAKSTPKAAACSGSTKSAA
                     TKQIKAAYDYFLNGTTKPPRTNAQREAYIAGMSDPALAALFEKGFGANAAAAATTNVK
                     VSAVTCTNKTTASVKADLVISGKVLPGVFPNPGSAVIENGVWKAGKLTFCDLTALSDS
                     TVTQSGPCSVQ"
     gene            complement(3202..>3440)
                     /locus_tag="VIJ44_02835"
     CDS             complement(3202..>3440)
                     /locus_tag="VIJ44_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164868.1"
                     /translation="KTKATFAFDLQLKQSSTGETTPPLGLRQPGAAVLKKGTWYVTAL
                     TVCDFAALSPDPANQPAVAECYQALGRQVPVPTT"
BASE COUNT          485 a         1189 c         1185 g          581 t
ORIGIN      
        1 catcaccctc atcctcttcg gtctcggcgc catcacctat gcccgccacc ccgaaggcat
       61 cgtcgagttc cagacccgca ggagcatcct ggagatggcc cggggccgcg ccctgggcgc
      121 acgagccaag gccctcgcgg ccgccaaaca actccccacg gcctacacac cggtctcgcg
      181 cgtcatcctt ggctgcgcgg ccggacccgg cctctacctc gcgtacctgc ttgtccggag
      241 cgccaccggg ctccacgcgc acttcaccgg gaccgctccg tacttgcggg tccagggcca
      301 gtgggccact gcccacagca ccacgctgct gtgcttcatc ttgccgagcg tcgccttcct
      361 cttggcctgg atgtggcgga ccgacaccgt gttgcgccgc cagcagggcg atcggcgcgg
      421 aaagctggtc atcatcggcg gcgccgggtt gggtgccttg ctcgggttcc tcttgcagtc
      481 gacgggtgat ctgccgggga gcgcgctcga ctgcgcgctc gtcgggatcc ccgtcggcgt
      541 cgccgtcggc gtgttcccgt ggcttccgca ccaggtcgag ctcgtcgccc gcgccaaggg
      601 ttggctcgag gcgcccatca cctggaaggt cggccggacc ccggcggtgt tcgtgctcac
      661 cggcgtctac ctgttctctc gtctgacgac ctcgaccccg cccacggggt ggccggtctg
      721 tctcgtggcg acggtgctga cgatcgtgtc gctccagtcg gtcggtggca tccaaggcgc
      781 ggtcaacgag cttgccatcg gcaatgaggg cgaagcgcgc cgtcgggccg aaggtctcgg
      841 tcaacctctg gtcggcgccc cgccagcggc gacggttgcc gcgccggtcg ggagtgcgga
      901 gtgagcgaga cgaccgctcg aacctcggag cggtcggtcc gtcccggcgc gctgctcgac
      961 gcggccggta tcacgaagcg cttcgccgga atcaccgccc tcaacggcgt caacctcgac
     1021 gtccagccgg gcgagatggt cgggctcatc gggccgaacg gggccggcaa gaccaccttc
     1081 ttcaactgcc tcctcgggat gctgcgcccc gaggggggca ccgtccactt cgacggccgg
     1141 gacatcaccc gcctcccggt ccaccggcgg gcccgcctcg ggttcgggcg gacgttccag
     1201 cgcctcgagc tgttttcggg gatgaccgtc cgggagcact tcctcgtcac cgaacgggcc
     1261 cggcgcggaa ccggtcggct ctggaaggac ttgttgaacc tggccaagcc cagcgtcgag
     1321 gaacaagcgc tcgccgaccg gatgatcgag atcgtcggac tccaggccga agccgacgag
     1381 ccggtcgagg ctctgagcct cgggcggggt cgcctggtcg agctgggtcg cgcgctcatg
     1441 acccagccga agctgctgct gctcgacgag ccgtcgtcgg gtctcgacgt ccgcgaaacc
     1501 gccagcctcg ccgacaagct ggccgcggtg cagacgcagc acgagttcgc ggtgctgctc
     1561 gtcgagcacg acgtcgagat ggtctcgaat ctcgtgacgc gcttgtacgt gttggacttc
     1621 ggtgagctga tcgccttcgg tccgacccag tcggtgctcg gcgacgaagc cgtccgcaag
     1681 gcctacttgg gagacgtggt gtgacgaacg acctcgcggc cgagaagccc ctcctggagt
     1741 tgcgcaacat cgaggccagc tacgggccgt tccggtcgct gttcggcgtg tcgttcgcga
     1801 tccaggaacg gggcgtcacc gcgctcctcg gcagcaacgg ttcgggcaag accaccgtcg
     1861 ttcggatctg ctccggcctg ttgcacccca ccggcgggca gctcctctac gacggcaacg
     1921 acgtgtccca gatgcgggcc tacaagctgg cccggctcgg cgtcgttcac gctccggaag
     1981 gtcggtccgt gtttgggacg ctctccgtcg aggagaacct ggccctcacc ttccgacagg
     2041 tgttcgggcg gcgcggctgc cgagaggcca ttgaccgctc ctatgagctg ttcccgcgtc
     2101 tcggggaacg gcgacgccag atcgcaggca cgctctcggg tggcgaacag cgcatgctgt
     2161 ccctcgcccg ggtcctggtc cgaggcccga agctgctcat caccgacgag ctctcactgg
     2221 ggctcgcccc ggtcatcatc gacgaggtct acgcgaccct cgcgacgatt cgggcgtcgg
     2281 ggacggcgct gctcatcgtc gagcagcaca tccgccaggc gttgacgctc gccgatcacg
     2341 tggtcgtgat ggccaagggt cgggtcagcc tctccggccg caccgacgag ctcggcgaca
     2401 tcgccgagcg gatcctcccc gccgtccacg gcgaggccgg ctccagcaac ggcgcctggt
     2461 cgtaacggtc accgcgattc gtggtggccg tggccagggg gccacgcgct cgccgcgcgg
     2521 ccccctgacg gacggctaca tcactgaacg gagcagggac cgctctgggt gacggtcgag
     2581 tccgaaagcg cggtcaggtc acagaacgtc agcttcccag ccttccagac gccgttctca
     2641 atgaccgcgg aacccgggtt cggaaagacg cccggcagga ccttgcccga gatgaccaag
     2701 tcggccttga ccgacgctgt ggtcttgttg gtgcaggtca cggcgctgac cttgacgttg
     2761 gtggtggcgg cagccgccgc gttggcgccg aagccctttt cgaacagcgc ggccagagcc
     2821 ggatcgctca tccccgcgat gtaggcctcg cgctgggcgt tggtgcgcgg cggcttcgtg
     2881 gtcccgttca ggaagtagtc gtacgccgcc ttgatctgct tggtggccgc ggacttggtg
     2941 cttcctgagc acgcagcggc cttcggtgtg ctcttcgctt cggcgctcga ggcaccgacg
     3001 acgagcatcc cgacggtcac gagcgcggtc agcaacgtga ctgctcggat tcgcatgttt
     3061 cccccattga tgttcgacac tatgacacca gtgtcagcaa tataaccggg gccctcacgc
     3121 gatggggcgc ggccctgcag cggccgatcg gggttgaggg acgccgcggg ggccgaggtc
     3181 ctcggcgcgt gtcacggccg gtcaggtggt cggaaccggg acctggcggc cgagggcctg
     3241 gtagcactcg gcgaccgcgg gctgattggc ggggtcgggg ctgagcgcgg cgaagtcgca
     3301 cacggtgagg gcggtgacgt accaggtgcc cttcttcaag actgccgcgc cgggctggcg
     3361 gagcccgagg gggggtgtcg tctccccggt cgaggactgc ttgagctgca gatcgaaggc
     3421 gaaggtcgcc ttcgtcttgg
//
LOCUS       DATCCH010000140         3222 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_471843, whole genome shotgun sequence.
ACCESSION   DATCCH010000140 DATCCH010000000
VERSION     DATCCH010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471843"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="VIJ44_02840"
     CDS             <1..408
                     /locus_tag="VIJ44_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164869.1"
                     /translation="FGLGTIVNTLSVGFIVDVLLRVVPSPDSAGGRWPMLLGGIVVTA
                     FGMGLYIGAGLGPGPRDGLMTGLAAKGHPLWLVRTVLELAALAIGWALGGDVGIGTVL
                     FAFAIGPLGHWFLARLHLGVDAVDPVPGATVGE"
     gene            405..1370
                     /locus_tag="VIJ44_02845"
     CDS             405..1370
                     /locus_tag="VIJ44_02845"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY5164870.1"
                     /translation="MTVRVGVIGTGFGARVVAPVFAATEGCEVVDVVSARDDAAVVAL
                     CENPDVDLVCVHSPPFLHASHVRRAMSEGRAVLCDKPFGLDGMEAQTLFAAAEAADCL
                     HLVNFEFRYDPTRVLVRDLVEGGSIGTPEHVEWTHLSAGSRQPLRPHGWLFDRAAGGG
                     WVGAWGSHAVDFLRWTFGDISEVWGRCRTTITERRDVDGAVRVCDAEDGFSAVLVSGD
                     GVTVALDSSFAAPVSLAPRFVVVGSDGVLECVADSRVVVRHRDGTRQEYESPHGDDAH
                     VEPMRRFAGVVRDAVREGIAPSGAPTFADGLACARVLERLRAGGE"
     gene            complement(1350..>3222)
                     /locus_tag="VIJ44_02850"
     CDS             complement(1350..>3222)
                     /locus_tag="VIJ44_02850"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY5164871.1"
                     /translation="HALVAARHGTEPGSSLDRLADRELLDLHEGEFRFPSEVVRDVAY
                     GTLTKAERARRHAVLADWLATGLTEDDGSTTALERVAHHYGAAALLLGELGVVDGVPA
                     DLPARALRLLEHAADRARNAELWPTASRLFEQALAVLPTDAPPEARWRIQIGRATALA
                     EQRELPAARAAVDEILDDDPDPRTTARALTVLADILQRLGDPDGAIETAERALECWME
                     LGDERGAADALRTRGITNMFIGNLDPAEQDISAALQAFRDGGDRRGEAWALQNLASIA
                     FFRGVPDQAEERLNRSAAMFRELGDYGGLHWCMGILAWVRFQQGRLEDAEALARDQLT
                     DTEAAGNRWVAGILTMLLANLALWSGRSQAAVTYANDTLARFDAIDDRWGQAQARASL
                     IRSLACLGHIDESLDLLDRFEVAAGREQTGMINRLLRAQVLIHVGAPEALAAALHVSG
                     DEGAGMQMLGDANLALGLALLQAGRVDEALGSMQTARGIAESEETGPGVAASAGFALG
                     AVAAGRIDEARRLADAGVGRGTYLDQIQHALAGAFARLRAGDPDTVTAFDQLVAFSDT
                     TEARLDQAVTRLARAHAWRALGRGDADEATRDAQVRLDAIGISATGWDQLFSLAAGT"
BASE COUNT          389 a         1025 c         1309 g          499 t
ORIGIN      
        1 ttcgggctcg ggacgatcgt gaacaccttg agcgtcgggt tcatcgtcga cgtgttgctg
       61 cgggtggtgc catcgcccga ctcggcgggg ggccgatggc cgatgctgct gggtggcatc
      121 gtcgtcaccg cgttcgggat gggcctctac atcggtgcgg gcctcgggcc cggtccccgc
      181 gacgggttga tgaccggcct ggccgcgaaa ggtcatccgc tctggttggt gcgcacggtg
      241 ctcgagctgg ccgcgctggc gatcggatgg gcgctgggcg gcgacgtcgg gatcggcacg
      301 gtcctgttcg cgttcgcgat cgggccgctc gggcactggt tcctcgcccg gttgcatctg
      361 ggtgtcgacg cggtggaccc ggtgccgggc gcgacggtcg gggaatgacc gtccgggtgg
      421 gggtcatcgg gaccgggttc ggcgcccggg tggtggcgcc ggtgttcgcg gcgacggaag
      481 ggtgcgaggt cgtcgacgtg gtgtcggccc gggacgacgc cgcggtggtg gcgctgtgcg
      541 agaaccccga cgtcgacctg gtctgcgtcc actcgcctcc gttcctgcac gcctcgcatg
      601 tgcgacgggc gatgagcgaa gggcgcgcgg tgctgtgtga caagccgttc gggctcgacg
      661 ggatggaggc ccagaccttg ttcgcggcgg cggaggcggc cgactgtctg cacctggtga
      721 acttcgagtt ccgctacgac ccgacccgcg tgctggtgcg ggacctcgtc gagggcggtt
      781 cgatcggcac gcccgagcac gtggagtgga cccacctgag tgcgggatcg cgacagccgt
      841 tgcggccgca cgggtggctg ttcgaccggg cggccggtgg cgggtgggtc ggggcgtggg
      901 ggtcgcacgc ggtcgacttc ttacgctgga ccttcgggga catcagtgag gtgtggggtc
      961 ggtgccggac gacgatcacg gaacggcgcg acgtcgacgg agccgtgcgg gtctgcgacg
     1021 ccgaggacgg gttcagcgcg gtgctggtgt ccggtgacgg ggtgacggtc gccctcgatt
     1081 cgagcttcgc ggcgccggtc agcttggcgc cgcgcttcgt ggtggtcggc agcgacgggg
     1141 tcctcgaatg tgtcgccgac tcgcgggtcg tggtgcgcca ccgcgacggc acccgtcagg
     1201 agtatgagag cccgcacggc gacgacgcgc acgtggagcc gatgcgacgc ttcgccggtg
     1261 tcgtgcggga cgcggttcgc gaggggatcg cgccgtcggg cgcgcccacg ttcgccgacg
     1321 ggctggcctg cgcccgggtg ctcgagcggt tacgtgccgg cggcgagtga gaagagctgg
     1381 tcccacccgg tggcgctgat cccgatggcg tcgaggcgga cctgggcgtc ccgggtggct
     1441 tcgtccgcgt caccgcgacc gagagcgcgc caggcgtgcg cccgggcgag gcgggtgacg
     1501 gcctggtcga gccgggcttc ggtggtgtcg ctgaacgcga cgagctggtc gaacgcggtg
     1561 accgtgtcgg ggtcaccggc ccggagtcgg gcgaacgcgc cggcgagggc gtgttggatc
     1621 tggtcgaggt aggtgccccg tccgacaccg gcgtcggcga gccggcgggc ctcgtcgatc
     1681 cgaccggcgg cgacggcacc gagggcgaac ccggcgctgg cggcgacgcc gggaccggtc
     1741 tcctcggatt ccgcgatccc gcgcgccgtt tgcatggagc cgagggcctc gtcgacgcga
     1801 ccggcttgga gcagggccaa gccgagcgcg aggttggcgt cgccgagcat ctgcatcccg
     1861 gcgccttcgt cgccgctgac gtgaagcgcc gcggcgaggg cctcgggtgc gccgacgtga
     1921 atgaggacct gggctcgtag gagccggttg atcatcccgg tctgttcgcg tcccgcggcg
     1981 acttcgaacc ggtcgaggag gtcgagggac tcgtcgatgt ggccgaggca ggcaagggac
     2041 cggatgagcg aggctcgggc ctgggcctgg ccccaacggt catcgatggc gtcgaagcgg
     2101 gccaaggtgt cgttcgcata cgtcaccgcg gcctgggacc ggccgctcca gagcgcgagg
     2161 ttggcgagca gcatggtgag gatcccggcg acccagcggt tcccggcggc ttcggtgtcg
     2221 gtcagctgat ctcgcgcgag tgcttcggcg tcctcgagac gaccctgttg gaaacgcacc
     2281 caggccagga tgcccatgca ccagtgcagg ccgccgtagt cgccgagctc gcggaacatc
     2341 gcggcggagc ggttcaggcg ttcctcggcc tggtcgggca ccccgcggaa gaaggcgatt
     2401 gacgccaggt tctgcagggc ccacgcctca cctcgccggt cgccgccgtc gcggaaggct
     2461 tggagcgcag ccgagatgtc ctgttcggcg gggtcgaggt tgccgatgaa catgttcgtg
     2521 atgccccggg ttcggagagc gtcggccgcg ccgcgttcgt cgccgagctc catccagcac
     2581 tcgagggcgc gttcggcggt ctcgatggct ccgtcgggat cgccgagccg ctgcaggatg
     2641 tcggccagga cggtgagggc ccgggcggtt gtgcgagggt cgggatcgtc gtcgaggatc
     2701 tcgtccaccg cggcccgggc cgccgggagc tcgcgctgct ccgcgagcgc agtggctcgc
     2761 ccgatctgga tccgccaacg ggcctccggt ggcgcgtcgg tggggaggac ggcgagggcc
     2821 tgctcgaaca ggcggctggc cgtgggccat agctcggcgt tgcgggcccg gtcggcggcg
     2881 tgctcgagga gccgcaacgc gcgggcgggc agatcggcgg gcacaccgtc gacgacgccg
     2941 agctcgccga ggaggagcgc ggcggcgcca tagtgatgcg cgacgcgctc caacgcggtg
     3001 gtcgacccgt catcctcggt gaggccggtg gccagccagt cggcgaggac cgcgtggcgg
     3061 cgagcccgct ccgccttcgt caacgtcccg tacgcgacgt cgcggaccac ctccgatggg
     3121 aagcggaact cgccctcgtg gaggtcgagc agttcgcggt cggcgagccg gtcgaggctc
     3181 gatccgggct cggtgccgtg acgggcggcg acgagcgcgt gg
//
LOCUS       DATCCH010000141         2855 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_49110, whole genome shotgun sequence.
ACCESSION   DATCCH010000141 DATCCH010000000
VERSION     DATCCH010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49110"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(552..675)
                     /locus_tag="VIJ44_02855"
                     /pseudo
     CDS             complement(552..675)
                     /locus_tag="VIJ44_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018890547.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
     gene            941..1558
                     /locus_tag="VIJ44_02860"
     CDS             941..1558
                     /locus_tag="VIJ44_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164872.1"
                     /translation="MTSDQHSNGARLLAVAALGALVLAACGGGSLSVSTPTSTSTTSS
                     TVASTSTTSTTSTTTTSTLPTPSTTSGPNTAQKVTFGGVQFGVPVGWPVYTLANDPTR
                     CVRQDVHAVYLGQEGPNPSCPARIVGHTETVHVEPFNAKSQPQAARATQAQVLNGLNV
                     RIDPAEQIAGAVTAVFPDQQLVVTITFVKSSVLADEIFQSFAHAS"
     gene            1548..2363
                     /locus_tag="VIJ44_02865"
     CDS             1548..2363
                     /locus_tag="VIJ44_02865"
                     /inference="COORDINATES: protein motif:HMM:NF020498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HEY5164873.1"
                     /translation="MRRNRARVRRATPLLAILAASVALAGCLPVRPPPPPPPGVFQGF
                     GFDACTAPTTGQMQAWLSSPFRSVGIYIGGGNRGCSQPALNASWVSTVGNEGWHLAPL
                     YVGLQAPCTSATNVSVINGFVPGVQGAQSALDAIHQANILGLGPGTPIYFDMEAYNSG
                     DAFCVEVVRQFVTNWVNELHLYGYVAGYYSSSASGIRDEAQIVQSPNYAHPDAIWFAN
                     WDNRLSTTGDPFFSDAYWFDHQRIHQFEGGHYEGWGGVALNIDSSVDDGPNAT"
     gene            2675..>2855
                     /locus_tag="VIJ44_02870"
     CDS             2675..>2855
                     /locus_tag="VIJ44_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002479078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEY5164874.1"
                     /translation="MVNGTVKFFNAEKGYGFISREDGDDVFVHFSQIEGEGYKTLQEG
                     QRVEFEIGPGRKGDEA"
BASE COUNT          470 a          958 c          966 g          461 t
ORIGIN      
        1 actcgccgcc ccagaatccg tattcacgat tggcgcgagc ccaacgccga catgggatca
       61 gcacccggca ggtcgcgcag agcgagcgcg ccgcgctctc gcgacgggct cggcgggccg
      121 ggcgttcccg gtcggccgcg aagtacaggt ggctgctacc ccgacatgtg gcctttgaga
      181 cccagctcag gtccggaggc cgtcgaggtt gtcgtcgagg tcggtccgga ccggcaccca
      241 cgaggcgtct tgaatggcca tcacgatgtg actcccggga ttcagcggat agtgaagccg
      301 tcggtcagac cgttgatgtc gaagacctgg tgggccggtt gttggcgtcc cgtgaggacg
      361 acgtacgttt tgggccccgc actgggtcga gtgaccagtg aggacccggc gttggcgcag
      421 gcctggtggc ggcggtgtcg accagcacac cttttcaagc gacggggacg tcgacacctt
      481 tcacgtgggc aggcggcggg cgatcccgag ccggtggtcg cgctggagcg gtggctgacc
      541 ccgcgctcat cgaccccgac cccctcgtcg gcccagggtc ctccggcgtc aacggatgtc
      601 ggtccgcagc ctcagtcccg aggtgagggt gcgaccacgc acagcgtcgg cctccacgag
      661 cgtccaaggg ccgtggcccg ggcgcattcc ccggtttgtc gcgctccgtg agggcccgga
      721 cgaatcgcgc agacttgccc gatatcgcca ccgtgacggt cgaaggaatc gacgatcggg
      781 cggtaatcat gggcccccgg caccgttcta aaggtgaagc gccgacaggt cgatgctcgt
      841 cgcgtcgggc cggaaggctc gggcgcgtca gggctcgacg ggagtcaagt gtttaggatt
      901 cgggagcccc aaatttccct cgtagctgag tcgacgcgcg atgacctctg atcaacactc
      961 gaacggcgcg cggctgcttg ccgtcgcggc gctcggcgcc ctggtgctcg ccgcgtgcgg
     1021 cggcggttcg ctcagcgtgt caacgccgac cagcaccagc acgacctcat cgacggtcgc
     1081 gtcgaccagc accactagca ccaccagcac gaccacgacc tcgaccctgc cgaccccgtc
     1141 gacgacgtcg ggccccaaca ccgcgcagaa ggtcaccttc ggtggtgtcc agttcggcgt
     1201 cccggtcggc tggccggtgt acacgttggc caacgacccg acgcgctgtg tccgccagga
     1261 tgtccatgcc gtgtatctcg gccaagaagg tccgaacccg tcgtgtccgg cgcggatcgt
     1321 cggacacacc gagaccgtcc acgtcgagcc gttcaatgcg aagtcacagc cccaggcggc
     1381 gcgggccacg caagcgcagg tcctgaacgg cctcaacgtg cggatcgacc cggcggagca
     1441 gatcgccggg gcggtcaccg cggtgttccc ggaccagcag ctcgtcgtca ccatcacgtt
     1501 cgtcaagagc tcggtgctcg ccgacgagat cttccagagc ttcgctcatg cgtcgtaacc
     1561 gggcgcgggt ccgccgcgcg actccgctgc tcgcgatctt ggccgccagc gtcgcgctcg
     1621 ccggctgctt gccggtgcgg ccgccgccgc cccctccacc cggcgtgttc cagggctttg
     1681 gcttcgacgc ctgcaccgcg ccaacgacgg gccagatgca ggcctggctg tcgtcaccgt
     1741 tccgcagcgt cgggatctat atcggcggag ggaaccgggg ctgctcgcaa ccggcgttga
     1801 acgcctcctg ggtcagcacc gtcgggaacg aagggtggca cctcgcgccg ctctatgtcg
     1861 gcctgcaggc tccctgtacg tcggccacca acgtcagcgt tatcaatggg tttgtgcccg
     1921 gcgtgcaggg ggcgcaatcg gcccttgacg cgattcacca ggccaacatc ctcgggctcg
     1981 ggcccgggac gccgatctac ttcgacatgg aggcgtacaa cagcggagac gcgttctgtg
     2041 tcgaggtcgt gcgccagttc gtgacgaact gggtcaacga gctgcacctc tacgggtatg
     2101 tcgccggcta ctactcgagc tcggcgtcgg gcatccgaga cgaggcccag atcgtccaga
     2161 gcccgaacta cgcgcacccc gatgcgatct ggttcgcgaa ctgggacaac cggctcagca
     2221 ccaccggcga cccgttcttc tccgacgcgt actggttcga ccatcaacga atccaccagt
     2281 tcgaaggcgg ccactatgag ggttggggcg gcgtggccct caacatcgac agcagcgtcg
     2341 acgacgggcc caacgcgacc taacggttcg accggcccgg cgcggtcgcg cccggcgact
     2401 ccgtcgagcc cgttcgacac cgcggccagc gtgaaatgag agcggttctc aacctggccg
     2461 gcgcgagtcc cggcggggac acgggcgcga tggcgctggc gtgtcgtcgg ggccagcggg
     2521 gccgcatcgt gtggacacgc gcacgcgagt gtcggtcgcg tcagatgtgg cacgggcacc
     2581 ccgcggccgg gtcataggtc cttgctaggg tctgcgcgtc caaaaggttg aagttgatca
     2641 gttggtcaac tgcaagagac aaggagttga tcgagtggtc aacggcacag tgaagttctt
     2701 caacgctgaa aagggttacg ggttcatctc gcgcgaggac ggcgacgacg tcttcgtcca
     2761 cttctcacag atcgagggtg agggttacaa gaccctccaa gagggccagc gggtcgagtt
     2821 cgagatcggg ccgggccgca agggcgacga agccc
//
LOCUS       DATCCH010000142         2924 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_513305, whole genome shotgun sequence.
ACCESSION   DATCCH010000142 DATCCH010000000
VERSION     DATCCH010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513305"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..217)
                     /locus_tag="VIJ44_02875"
     CDS             complement(44..217)
                     /locus_tag="VIJ44_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164875.1"
                     /translation="MLVCTGCAKTVLITIRMRVAGREIVFHRCSKCEANTWQDDEGVL
                     SLTEVLDLARARQ"
     gene            complement(335..1063)
                     /locus_tag="VIJ44_02880"
     CDS             complement(335..1063)
                     /locus_tag="VIJ44_02880"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEY5164876.1"
                     /translation="MDPGTSLPPERVSAAGSVPLRWGLGDFLWIYVAGIVASVVGASI
                     GFAITGDTSGHIGAVTTALSTLGQFGTWFALVVLIARSKGRSVRDDFGLTLQLRDWWA
                     PFAGLGLFLLGTALIYPLVNIVNAHQQVVDDLQRASGAKLAVFAVVAAIVAPICEELL
                     FRGLLLRALRRRMSPVMAVIVQALAFALAHPMLSPTLGDFAVVPALFLLGVVSGVVAV
                     RRGDISASILMHIGFNLVTTLIAL"
     gene            1342..>2924
                     /gene="ftsH"
                     /locus_tag="VIJ44_02885"
     CDS             1342..>2924
                     /gene="ftsH"
                     /locus_tag="VIJ44_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEY5164877.1"
                     /translation="MVIGLIVAIVLVWKAAPTSTTPQAKLDYGAFLTLVSQDHVSNIK
                     YDASSGKITGAFKDGFTQDNGKKEFTTQGQINQLPDADIQLLNAHGVARNYKPRSSDV
                     LGTLLVYLLPLVLIGAIWFFIARRAQGQMGAVMNIGRSRAKVYNTDKPKTTFSDVAGY
                     GAVKEEITEVVGFLKQPGKFKEIGARIPKGVLLVGPPGTGKTLIARAVAGEAGVPFIS
                     ITGSDFMEMFVGVGAARVRDLFQTAKKQAPAIIFVDEIDSIGRKRGAGLGGGHDEREQ
                     TLNQMLAEMDGFEATEGIVMMAATNRPDVLDPALLRPGRFDRQILVPLPTLDERVEIL
                     KVHMRDKKIDPDVDVGVVARGTPGMSGADLANLVNEAALFAVRRNAQEVHREDFDAAR
                     DRVLMGLKRESLALSEEEKEIIAYHEGGHAVCAYVLEHADPLHKVTILPTGMALGVTH
                     QLPVEERHIYKREYIADSLVVRLGGRVAEELIFGHQSTGAQNDLVGNTELARKMVREW
                     GMSDRIGPMSWGSQGAVFLG"
BASE COUNT          570 a          968 c          963 g          423 t
ORIGIN      
        1 acgccggact cacccccacg gtccgtgggg ccgcgagaac ggcctactgg cgggcgcggg
       61 cgaggtcgag gacctcggtc aacgacagga cgccctcgtc gtcctgccag gtgttggcct
      121 cgcacttcga acaacggtgg aacacgatct cgcggcccgc aacgcgcatg cggatcgtga
      181 tgagcaccgt cttcgcacat cctgtgcaaa ccagcattcc gtgcctccct tgacaccgcg
      241 ccggtcttca ttgtcggcac cgaggtgcga gaactgaatc acccggacgg gtgaccttcg
      301 ccgccccggc gccgcccggg gaccaaaccg ccggttagag ggcgataagt gtcgtgacga
      361 ggttgaaacc gatgtgcatc aggatcgagg ccgagatatc gccgcggcgg accgcgacca
      421 cccccgacac gacccccagg aggaagaggg ccgggaccac ggcgaaatcg ccgagggtcg
      481 ggctcagcat cggatgcgcg agcgcgaagg cgagcgcctg cacgatcacc gccatgaccg
      541 gcgacatccg gcggcgcagg gcccggagca atagcccccg gaacagcagt tcctcgcaaa
      601 tgggcgcgac gatcgccgcg accactgcga acaccgcgag cttggcacca ctcgcccgtt
      661 ggaggtcgtc gacgacctgt tgatgcgcgt tcacgatgtt gaccagcggg tagatcaacg
      721 cggtgccgag aaggaagagc ccgagcccgg cgaagggagc ccaccagtcc cgcagctgca
      781 gcgtcaggcc gaagtcgtca cggaccgatc tgcccttgga gcgcgcgatg agcaccacca
      841 acgcgaacca ggtcccgaac tgaccgaggg tcgacagggc cgtggtgacg gcgccgatgt
      901 ggccggaggt gtcgcccgtg atcgcgaatc cgatcgacgc gccgacgacc gaagcgacga
      961 tgccggcgac gtagatccac aagaaatcgc cgagacccca tcggagcggc acggaacccg
     1021 ccgccgacac ccgttccggc ggcaaggaag ttcccggatc caccggctca ggctaccgag
     1081 cacgcgattg cggccgatcg acacctggcg gtgagagatc gtccgatcga ggacggcgcg
     1141 aacccggatt cgaccgggaa caggaaacgt ctctcgtccg ccatacgatg ggaagtcttc
     1201 gactgccgtg gagaatccct catgagccca ggccctgaag accagtggcg cccgccggaa
     1261 ccaccgccgg gtcgttcacc gcggccgggc tcacccggag gaccgatccg gtccggtaac
     1321 cggccgaaat ggatgccgtg ggtggtcatc ggattgatcg tggccatcgt cttggtgtgg
     1381 aaggcggcac cgacttccac gaccccgcag gcgaagctcg actacggagc cttcctcacg
     1441 ctggtcagcc aagatcacgt gtcgaacatc aagtacgacg cgtcgagcgg caagatcacc
     1501 ggcgcgttca aggacggctt cacccaggac aacggcaaga aggaattcac cacccagggt
     1561 cagatcaacc aactgccgga tgccgacatc cagttgttga atgcccacgg ggtcgcgcgc
     1621 aactacaagc cgcgctcgag cgacgtgctc ggcactctgc tcgtctatct cctgccgctg
     1681 gtgctcatcg gggccatttg gttcttcatc gcccgacggg cccaaggcca gatgggcgcg
     1741 gtgatgaaca tcgggcggtc ccgcgccaag gtgtacaaca ccgacaaacc gaagacgacc
     1801 ttctccgacg tagccggcta cggcgcggtg aaagaagaga tcaccgaggt cgtcggcttc
     1861 ttgaagcaac ccggcaagtt caaggagatc ggcgcccgga ttccgaaggg cgtgctgctc
     1921 gtcggaccac ccggtaccgg taagacgctc atcgcccgcg ccgtcgcggg cgaggcaggc
     1981 gttcccttca tctcgatcac cggctccgac ttcatggaga tgttcgtcgg ggtcggcgcg
     2041 gcgcgggtgc gcgacctgtt ccagaccgcg aagaagcagg cgccggcgat catcttcgtc
     2101 gacgagatcg actcgatcgg ccgcaaacgc ggcgcgggcc tcggtggtgg ccacgacgag
     2161 cgtgagcaga cgctcaacca gatgctcgcc gagatggacg gcttcgaggc caccgagggc
     2221 atcgtgatga tggcggcgac caaccggccc gacgtcctcg atcccgcgct cttgcggccc
     2281 ggccgattcg accgtcagat cctcgtgccg ttgccgacac tcgacgagcg ggtcgagatc
     2341 ctcaaggtcc acatgcgaga caagaagatc gacccggacg tcgacgtcgg cgtcgtggcg
     2401 cgcggaacac ccggaatgag cggcgccgat ctggccaacc tcgtgaacga agccgccctg
     2461 ttcgccgtgc gacgcaacgc acaggaagtg caccgcgaag acttcgacgc cgcgcgcgac
     2521 cgggtcctga tggggttgaa gcgcgagtcg ctcgcgttgt ccgaagagga aaaggagatc
     2581 atcgcctacc acgagggtgg ccacgcggtg tgtgcgtatg tgctcgagca cgccgatcct
     2641 ctccacaagg tgacgatcct cccgacgggg atggcgctcg gtgtcacgca ccagctgccc
     2701 gtcgaagagc gtcacatcta caagcgcgag tacatcgccg actcgctggt ggtacgcctg
     2761 ggtggtcgag tcgccgagga gctgatcttc ggccaccaat cgacgggcgc ccagaacgac
     2821 ctcgtgggca acacggagct cgcccgcaag atggtgcgcg agtggggcat gtccgaccgc
     2881 atcggtccga tgtcctgggg ttcccagggt gcggtgttcc tcgg
//
LOCUS       DATCCH010000143         6009 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_554729, whole genome shotgun sequence.
ACCESSION   DATCCH010000143 DATCCH010000000
VERSION     DATCCH010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6009
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554729"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="VIJ44_02890"
     CDS             complement(<1..568)
                     /locus_tag="VIJ44_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020554454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY5164878.1"
                     /translation="MTVPFAPGSVSVRLYPHNDLPASQIVTELRVQAALAVGHGFDGV
                     MTSEHHGGFAGYLPNPLQVAGWCLDAMAAGWAAPCPLLLPLRPAALVAEEVAWLAARF
                     PDRVGVGVAAGALPADFEIMGLTMEDLTDRFTSGLERLVGFLHGREPGALAGDPAVAA
                     CAQAPVPIVSAAMGFTAVRRAARLGIGLL"
     gene            complement(571..723)
                     /locus_tag="VIJ44_02895"
     CDS             complement(571..723)
                     /locus_tag="VIJ44_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164879.1"
                     /translation="MASCSFEVMMGDPRSFPADPAGYETLLAERGIEQLHDPPIAMPG
                     LEDSRS"
     gene            933..1088
                     /locus_tag="VIJ44_02900"
     CDS             933..1088
                     /locus_tag="VIJ44_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164880.1"
                     /translation="MLQLAGPPVPDVARGAQRHPTGGMTQIRDRLERANLVARGAAPS
                     DRRQVLV"
     gene            complement(1268..2029)
                     /locus_tag="VIJ44_02905"
     CDS             complement(1268..2029)
                     /locus_tag="VIJ44_02905"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014214392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5164881.1"
                     /translation="MTGLLTGRGVLVTGAGGGVGRGIAFACAAAGADVVVASPRENGA
                     ETVAEISARGGSAIWARCDVTNPGDVRAAVATAEARRGLHALVHNATSRRSSEPARIE
                     DLDAALWEDHAGVSLRGALHCARAAWPELRAHRGRFILMTSPAGMEGSLMLPAYGIVK
                     AALRGFAKSLAREWAPDGVTVTVVSPLAETPAMTNAYVKDPELAARLARRVPLGHLGD
                     PETDIGPVVVFLASDDARYVTGQTLPVDGGRFMTL"
     gene            complement(2026..2811)
                     /locus_tag="VIJ44_02910"
     CDS             complement(2026..2811)
                     /locus_tag="VIJ44_02910"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5164882.1"
                     /translation="MATPSDGDADRIVAPGLLAGRVVLVTGGARGLGRDVALGLADAG
                     AAVGVVDSAATAPVAESIIGAGRRAVAVDAAIETQEEASHAFAAVTEALGPLDAVVHA
                     SVDPDALVPSRIVDTADADWDRRCEAPLRAALVCAEAAFSQLQARGGRLVFVTPTVAL
                     TGAAGLVAYTTAVEGIRALAKSAARQWGTRAITVNCVAPPLELLAADGRGAPEPTVGD
                     PALGRAPHGRVDVAPVVAWLAGPGAGFVTGLTVTVDGGIVMAP"
     gene            2855..3727
                     /locus_tag="VIJ44_02915"
     CDS             2855..3727
                     /locus_tag="VIJ44_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5164883.1"
                     /translation="MDTREAEDHMIGERLNLSGRSVVVAGAGGGGIGTAVCRFLAEAG
                     ATVVAWDLDPDRLAVSERALRDLNGPHHFAVVDVRDADAVDQAVRAAGRLHGLVHVAG
                     GLPPDQWAPLLEVTPAVFDEVIHLNLASAFVTSKAVAAQLVEHGTGGSIVHIASIAGL
                     SAMPFGAAYSAAKAGMIALTRTAALEWGPFGIRVNAVAPGTVRVPRNRSESADEDTVE
                     ERTAIPLGRRGEPEDIAGAVVFLLSDLAGFVTGQVLAVDGGSSARPSYLDAANLPVFV
                     HDAGLRARLGALGR"
     gene            3771..4268
                     /locus_tag="VIJ44_02920"
     CDS             3771..4268
                     /locus_tag="VIJ44_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018832422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY5164884.1"
                     /translation="MSDDPFGGRSGERAMLGGFLDWYRAVVARKVEGLAEDDAARVMT
                     PSGVSPLGVVRHLAWVERLWFQWRLAGEDVDLFTGPDNSVTFRLEPTDTVGLVVATYR
                     AEIEQAQRVVAAVGSLDDLGVRPHPLFGLVSARWVLVHVIEETARHAGHLDVMREQLD
                     GATGD"
     gene            complement(4485..5576)
                     /locus_tag="VIJ44_02925"
     CDS             complement(4485..5576)
                     /locus_tag="VIJ44_02925"
                     /inference="COORDINATES: protein motif:HMM:NF013954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEY5164885.1"
                     /translation="MVLGVSVLVVGVPLRVGAQTTPPAPQDELTAVAIELSRTTASLD
                     VVARAAADAQVRLASAQAILADVEARTAATTAQIDALRAQLVGRAAQVYEQRSNDGAV
                     LRVQHVVDLAAGEQYTNAAAIDGTRQLQQLNVVETQLEQEQAQAAATRQAIADDELRL
                     ESSRSNLEAVRARDQALLDRVGAIPIMGDARLTAAQIAAWFHSTGQVAHLAGGTTIDD
                     LAAMYIDEGAAEHVRGDVAFAQAIIETGSFGHALDNNYSGIGACDSCTSEISFPTPRD
                     GVRAQIQLLRSYADPGSRAALLAHPPEPALFGGTPAAAAASYDSFAYKGSAPVWNVMG
                     NGKWATDPDYSTKVLEIYARMVGFASTHG"
     gene            complement(5800..6000)
                     /locus_tag="VIJ44_02930"
     CDS             complement(5800..6000)
                     /locus_tag="VIJ44_02930"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HEY5164886.1"
                     /translation="MRDAHVTPEELSEACREHGVGDPRDCHLVVLEIDGSISVVPRSA
                     EAPSPPSRRPARRRIGRVDRGE"
BASE COUNT          862 a         2126 c         2174 g          847 t
ORIGIN      
        1 agagcaaccc gatgccgaga cgcgccgcgc gtcgcaccgc ggtgaagccc atcgctgcgc
       61 tcacgatcgg gaccggcgcc tgcgcgcaag ccgcgacggc cgggtcaccc gcgagggccc
      121 ccggctcacg gccgtgcagg aacccgacca ggcgctcgag tccactcgtg aaccggtcgg
      181 tgaggtcctc catcgtcaag cccatgatct cgaagtccgc cgggagcgcg cccgcggcca
      241 caccgacgcc cacccggtcg gggaagcgtg ccgcgagcca ggcgacttct tccgcgacca
      301 gcgcggcggg acgcaacggc aggagcagcg ggcagggcgc ggcccatccg gcagccatgg
      361 cgtcgaggca ccatccggca acttgaaggg ggttggggag gtagccggcg aacccgccgt
      421 gatgctcact cgtcatcacc ccatcgaagc cgtgcccgac agccagcgcc gcctgcaccc
      481 gcaactcggt gacgatctgc gacgcaggca ggtcgttgtg cgggtagagg cgcactgaca
      541 ccgacccggg cgcgaatggg acggtcaccc tcagctccgg ctgtcctcga gcccgggcat
      601 ggcgatcggc gggtcgtgca gctgctcgat gcctcgttcg gccagcagag tctcgtagcc
      661 ggccgggtcg gcgggaaacg agcggggatc gcccatcatc acctcgaaag agcacgacgc
      721 catcgggacc ggcgacgaac ggaccgaacg tgtcaccctg gtcgagcgcg atatgcgtcc
      781 cgggcgggca gctcatctcg ccttcggtgg cgaacactac ctgggcgcgg cgatgaccgt
      841 gcgcgtgcac gatcctcccc ggatcccacc ggacaaatgg cgacaggtag cgaggcgaga
      901 actccagcca cttctcacgc acggtgcggc gcgtgttgca gctcgccggg ccccccgtac
      961 cggacgtcgc ccgcggagcc cagcgacacc cgactggtgg catgacccag atccgcgacc
     1021 ggctcgagcg ggccaacctc gtcgcccgcg gcgctgctcc gtcggatcgc cgccaggtcc
     1081 tggtctaagc actgaggggc tgcgcaccgc cgagaaggcc gacgcccgct acgccgacga
     1141 acgacagcaa ctcctcgccg tgctgtcacc cggcgaggtc gaagcgatcg atgacgccac
     1201 caaccggccc ctcggggtcc tcgtcgagga cgcccgcgcc cgccagtgac gcactgcagt
     1261 cgccaggtta gagcgtcatg aagcggccgc cgtcgacggg cagcgtctgg ccggtcacgt
     1321 accgagcgtc gtcgctggcc aggaacacga cgaccggtcc gatatcggtt tcgggatcgc
     1381 cgaggtgccc gagtgggact cgtcgggcca gccgagccgc cagctcgggg tccttgacgt
     1441 aggcgttcgt catcgccggc gtctcggcca gcggtgacac caccgtgacc gtcaccccgt
     1501 cgggggccca ctcccgagcg aggctcttcg cgaagccgcg caacgcggcc ttgacgatcc
     1561 cataggccgg gagcatcagg ctgccttcca tccccgcggg cgacgtcatg aggatgaacc
     1621 tgccgcggtg cgcgcgcagt tcgggccatg cggcgcgggc gcaatgcagg gcgccgcgga
     1681 gcgagacgcc ggcatggtcc tcccagagtg ccgcgtcgag atcctcgatg cgggccggtt
     1741 cactcgagcg gcggctggtc gcgttgtgaa ccaacgcgtg gagtccacga cgagcctcgg
     1801 cggtggcgac ggcggctctg acgtcgccgg ggttcgtgac atcgcagcgc gcccagatcg
     1861 ccgagccgcc ccgggcgctg atctcggcga cggtctcggc gccgttctcg cgtggtgatg
     1921 ccacgacgac gtcggctccg gccgcggcgc acgcgaacgc gatgcctcga ccgacgccgc
     1981 cgccggcgcc ggtcaccagc actccccggc cggtcaggag accggtcatg gggccatcac
     2041 gatcccgccg tcgacggtga cggtcaggcc ggtcacgaac cccgctccag gaccagccag
     2101 ccaagcgacg actggcgcca cgtcgacccg accgtgcggt gcgcgaccca acgcggggtc
     2161 cccgacggtc ggctcgggcg cgccgcgtcc atccgcggcg agcagctcga gcggtggcgc
     2221 gacgcagttc accgtgatgg cgcgggtgcc ccactggcgc gcggcggact tggccagcgc
     2281 tcggatgccc tcgaccgccg tcgtgtatgc gaccagcccg gcggcgccgg tcagcgccac
     2341 ggtcggtgtc acgaacacga gccggccgcc gcgggcctgg agctgggaga aggcggcctc
     2401 agcgcagacc agcgcagctc ggagtggtgc ctcacatcgc cggtcccaat cggcgtcggc
     2461 ggtgtcgacg atgcggctcg ggacgagcgc gtcggggtcg accgacgcgt ggacgacggc
     2521 gtcgagcggc ccgagcgctt ctgtgaccgc cgcgaacgcg tgcgaggcct cctcttgcgt
     2581 ctcgatggcc gcgtcgaccg ccacggcgcg acgtcccgcc ccgatgatgg actccgcgac
     2641 gggtgcggtg gcggcgctgt cgacgacgcc gaccgcggcg cccgcgtccg cgaggccgag
     2701 cgcgacatcc cggccgaggc cgcgggcgcc gccggtcacc agaacgaccc gcccggcgag
     2761 cagcccagga gcgacgattc ggtccgcatc tccgtccgac ggcgtcgcca tcccccttcg
     2821 ccgggcccgg cgcgggatgc tatcggcgcg cccaatggac acccgggagg cggaggacca
     2881 catgatcggc gaacgactga acctttcggg ccgctcagtc gtcgtcgcgg gcgcaggcgg
     2941 cggcggaatc ggcaccgcgg tgtgtcgttt cctcgccgag gcgggagcca ccgtcgtcgc
     3001 ctgggacctc gaccctgacc gcctggccgt ctccgagcgc gcactgcgcg acctcaacgg
     3061 cccccatcac ttcgccgtcg tcgacgtacg cgacgccgac gcggtcgacc aagcggtgcg
     3121 tgccgcaggg cgcctgcacg gcctcgtcca tgtcgcgggt ggcttgccgc ccgatcagtg
     3181 ggcaccgcta cttgaggtca cgccggccgt attcgacgaa gtgatccacc tcaacctggc
     3241 gtcggcgttc gtcacctcga aggcggtagc ggcccagctc gtcgagcacg gcacgggcgg
     3301 gagcatcgtg cacattgcgt cgattgccgg actgtccgcc atgccgttcg gcgcggcgta
     3361 ctcggccgcg aaggccggga tgatcgcgct gacgcgaacg gcggcgctcg agtggggacc
     3421 gttcgggatc cgcgttaacg cggtggcgcc gggcaccgtc cgagtgccgc gcaaccggtc
     3481 cgagagcgcc gacgaggaca ccgtcgaaga gcgcaccgcg atcccgctcg gacgtcgggg
     3541 cgagcccgag gacatcgccg gcgcggtggt gttcttgctc tccgatctcg ccggcttcgt
     3601 caccggtcag gtcctcgcgg tcgacggggg gtcgtcggct cggccgtcgt acctcgacgc
     3661 cgcgaacctc ccggtgttcg tccacgacgc cgggctgcgg gcccgactgg gggccctcgg
     3721 tcggtagcaa cgctcgcgct gctccatctc cgacctctac gatccccgac gtgagtgacg
     3781 acccgttcgg cggtcgcagc ggcgaacggg ccatgctcgg cggctttctc gattggtacc
     3841 gggcggtcgt ggcccgcaag gtcgagggcc tcgccgagga tgacgcggcg cgggtcatga
     3901 caccgagcgg ggtcagcccc ctcggggtgg tccggcacct cgcctgggtc gagcggctct
     3961 ggttccagtg gcgactcgcg ggtgaggacg tggacctctt cacgggtccg gacaactcgg
     4021 tcacgttccg gctcgagccg acggacaccg tcgggttggt cgtcgccacg taccgagccg
     4081 agatcgaaca ggcccagcga gttgtcgccg ccgtcgggtc cctcgacgac ctcggcgttc
     4141 gcccgcatcc gctcttcgga ctcgtgagtg cgcggtgggt gctcgtgcac gtcatcgagg
     4201 agaccgctcg tcacgccggc catctggacg tgatgcgtga acagctcgac ggtgccaccg
     4261 gcgattgaac ccgccgcggc ggctgcgtcg ggcgcgtccc ggacgcgcca gtacggatgg
     4321 tcaggaaccg catctcgacg ggaccggcgg tgaagccgta ctcgtagcgg ccggggaaca
     4381 cgatcgacga cgacggtgac cgcccacttc gcgcgactgg gagcagccgc cgaagaagga
     4441 atggcagcgt cggttcgtcg gcggctccga cgatccggcg gtgctcagcc gtgcgtcgag
     4501 gcgaacccga ccatccgggc gtagatctca aggaccttgg tcgagtagtc gggatcggtc
     4561 gcccacttcc cgttgcccat gacgttccac accggcgccg agcccttgta cgcgaacgag
     4621 tcgtacgacg ccgcggcggc cgcgggggtg cccccgaaga gggccggctc gggtgggtgc
     4681 gcgaggagcg cggcgcggct gccgggatcg gcgtagctgc gcagcagctg gatctgggcg
     4741 cggaccccgt cgcgcggggt ggggaacgaa atctcactgg tgcagctgtc gcaggcgccg
     4801 atccccgagt agttgttgtc gagggcatgc ccgaacgagc cggtctcgat gatcgcctgc
     4861 gcgaacgcga catcgccccg gacgtgctcg gctgcgccct cgtcgatgta catcgcggcg
     4921 aggtcgtcga tggtcgtccc gccggcgagg tgtgcgacct gcccggtcga gtggaaccag
     4981 gctgcgattt gcgccgcggt gaggcgcgcg tcgcccatga tggggatcgc tcccacgcgg
     5041 tcgagcaggg cctggtcgcg ggcccggacc gcttcaagat tggaccggga cgactccagc
     5101 cggagctcgt cgtcggcgat ggcctgacgc gtcgcggcgg cttgagcctg ctcttgttcg
     5161 agttgggtct cgaccacgtt gagctgttgc agctggcggg tcccgtcgat cgcggccgcg
     5221 ttggtgtact gctcacccgc ggcgaggtcc acgacgtgct ggaccctgag caccgccccg
     5281 tcgttgctgc gctgctcata cacctgcgcg gcgcgcccga ccagctgcgc gcgcagtgcg
     5341 tcgatctggg ccgtggtggc cgcggtgcgc gcttccacgt cggcgaggat ggcttgcgcc
     5401 gaggccagac ggacctgcgc gtcagcggcc gcgcgggcga cgacatcgag gctggcggtg
     5461 gtgcgcgaca actcgatcgc gaccgcggtc agctcgtctt gcggtgcggg cggggttgtc
     5521 tgggctccga ctcgcagcgg tacgccgacg accaagaccg agacaccgag caccatgggt
     5581 gcacggagaa cggttcgcca acgatggagg tgggtcacgg tcgtcacgga cggtcgggcg
     5641 ggcctgcggc tcgctgcggc tcgtaggtat tgacggcgtc ggagcggttc aacttgacga
     5701 aaaactgggc cccggcgcgc ctcacccggg tttcgacacg aagaactcga tcctgtgatg
     5761 ccaggcgccg ctcagcgtga gaggcggccc gcctcggcgt cactctccgc ggtcgacacg
     5821 cccgattcgg cgccgagcgg gacgcctcga tggcggggag ggagcctcgg cgctcctcgg
     5881 gacgacgctg atcgatccgt cgatctcgag gacgaccagg tgacagtcgc gcgggtcgcc
     5941 gacgccatgt tcccggcacg cttcggagag ctcttcggga gtgacgtgcg cgtcccgcaa
     6001 cggggcggt
//
LOCUS       DATCCH010000144         5655 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_513845, whole genome shotgun sequence.
ACCESSION   DATCCH010000144 DATCCH010000000
VERSION     DATCCH010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5655
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513845"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..894)
                     /locus_tag="VIJ44_02935"
     CDS             complement(109..894)
                     /locus_tag="VIJ44_02935"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEY5164887.1"
                     /translation="MSTLQIAPIDGPLGATATGVDLHQALDPADEHTLRAALADRLLL
                     VVPGQFLDVDAERRVARTFGQLWEHPAARHRAGRGSAAVLDSRRTPDRGRPAEVWHAD
                     ATFTARPPAYTVLSAQIVPDHGGATSFANQQLAYTHLDDEWRDRLTDRRAVHAPGPRQ
                     LRRAPWLTESTHPVVRVDTETGRSSLFVNPGFTRRFEHLTVEQSRPYLRALFRVALDP
                     SVVYDHHWNAGDLVIWDNRALLHRAHHDHGDEARVLARVTVAA"
     gene            complement(952..2346)
                     /gene="murD"
                     /locus_tag="VIJ44_02940"
     CDS             complement(952..2346)
                     /gene="murD"
                     /locus_tag="VIJ44_02940"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEY5164888.1"
                     /translation="MTPASLAGSRVLIMGIGRRAGGAGAIRYAVESGAQVRVTDRGDP
                     ASFHDIVEQFAGTPVEFVLGHHDEVDFGWADVVIRNPDVRRDSPYLRVAEEHGARIEM
                     EMTLFLAACAAPTIGVTGTKGKTTTAMLTHSILRERWPGTVVAGNMGRSALARLGDVD
                     PDVPVILELSSFQLEGLGEHALAPTVAVITNVRPDHLDRYPSFDAYADAKAQIVRHQH
                     THDWKVVPADDPAVDALVSGTPAPPITFGDDPVGPRTLFVADGHFVASWDGGDLDLGP
                     VDALRVPGAHNRRNALAAAGAALAVGVTADEVRRGVARFDGVPHRLEPVATIAGVDFV
                     NDTTATTPEDAEAALRAFPGRPVVAIAGGFDKGLDLDALVAALNDQAVATVLLAGSAT
                     PGLQDRLARDTTAGPFDSMRDAVQAAVSLAPDGAVVLLSPGCASFGLFVDEFDRGDQF
                     RDAVHALEPGRRPR"
     gene            complement(2343..3866)
                     /locus_tag="VIJ44_02945"
     CDS             complement(2343..3866)
                     /locus_tag="VIJ44_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEY5164889.1"
                     /translation="MTFDPIVTPTVPVAPHREPHFVHDEARDAARQRLDDYRARRGRN
                     PNVLVILFDDVGWGDFGCYGGGVAVGAPTPNLDRLARDGLLLTSCYSEPSCSPSRASL
                     MTGRLPMRHGLLRPPMYGEPGGLEGEVTLAELLSSAGYVTQAVGKWHMGENAASQPQH
                     VGFDDFYGFLSVSDMYTEWRDPYFFPEIVYSDERTEWIKNQPFNRCFVHATRGADVEN
                     VEEVTIPVLSQLDDRWCDYSIGFLERMAGGGSPWFLYHCTRGAHFDNYPHQRFLGVSP
                     ARHPYKDTIVELDDIVGRLLETLRTTGQLEDTFVFVSSDNGPEMETWPDAAYSPFRSA
                     KGSTWEGGVRVPALFSWPGVVDAGQASDGLFAFTDVLPTVVTLAGCADSVPTDRFIDG
                     VDQTSFLLAPGAPSNRKYHYYWLLDRFSAVRVGEYKFMLESISDDDHDVLNPGGFTGV
                     TQRYPYGRLYNLYLDPKETRSYLIRKLAYIDSFRNGIQDHLLTFRDYPPKRVVGGSA"
     gene            4069..4287
                     /locus_tag="VIJ44_02950"
     CDS             4069..4287
                     /locus_tag="VIJ44_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164890.1"
                     /translation="MTDASCASCGTTDGTVRIRPWHELDVALCARCYASLTGDHDNHG
                     VGFTLAWVVLVTGLLLVAAGAATYLLTR"
     gene            complement(4288..5292)
                     /locus_tag="VIJ44_02955"
     CDS             complement(4288..5292)
                     /locus_tag="VIJ44_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY5164891.1"
                     /translation="MELRPLGPTGLRVAPLCLGAMNFGDPTEPAESARIIDAALDAGV
                     TMIDTADVYAGGTSEQIVGDALTANGRRDDVVLATKVGMPRGKAAPGTWHRREHIIDS
                     CETSLRSLQTDHIDLYQLHRPSMAVPQAETLAAFDELVQAGKVRHVGCSTHPAWMVME
                     ALAIAARDGRPGYATEQPPYNLLDRRIENELLPLCRAQGLGVLPWSPLGGGILAGRYQ
                     DVDQIPDDSRAARRPQLRDRVTARALEVARGVAALAADRGLTSSQLALLWVKDQPDVT
                     APIVGPRTLAQLEEALGILDRSLDDDARVACDELVHPGSAVADFFNTSGWMREQISAA
                     "
BASE COUNT          800 a         1839 c         2192 g          824 t
ORIGIN      
        1 gggccgtggc agccgggcgg gatcagcgcg ttccagtggc gcaccggcga ggagcaggcc
       61 cgacgcgagg gacgcgccta cgagccgttc ggtgccgcgg tgctgtgatc aggccgcgac
      121 ggtcacccgg gcgaggaccc gggcctcgtc gccgtggtcg tggtgggccc ggtgcagcag
      181 cgcccggttg tcccagatga ccaggtcacc ggcgttccag tgatggtcat agacgacgct
      241 cgggtcgagc gcgacgcgaa acagcgccct caggtacggg cggctctgct cgacggtgag
      301 gtgctcgaac cggcgggtga agccggggtt gacgaacagc gacgatcgcc cggtctcggt
      361 gtcgacgcgg acgacggggt gtgtcgactc ggtgagccac ggggcgcgtc ggagctggcg
      421 ggggccgggg gcgtgcacgg cgcgtcggtc ggtgaggcgg tcccgccact cgtcgtcgag
      481 gtgggtgtag gcgagctgct ggttggcgaa cgacgtggcc ccgccgtggt cggggacgat
      541 ctgggctgag aggacggtgt atgccggggg gcgggcggtg aaggtggcgt cggcgtgcca
      601 cacctcggcc ggtcggcccc ggtcgggcgt ccggcggctg tcgagcaccg cggcgctgcc
      661 ccgcccggcg cggtgacggg ccgcgggatg ctcccagagt tgcccgaagg tgcgggcgac
      721 gcggcgctcg gcgtcgacgt cgaggaactg gcccgggacg acgaggagga gccggtcggc
      781 gagcgcggcg cgcagggtgt gctcgtccgc cgggtcgagg gcctggtgaa ggtcgacgcc
      841 ggtggcggtc gctccaagcg gtccgtcgat gggtgcgatc tgcagggtgc tcatgggagg
      901 aatcgtcgcg ccggtccgtg aagcatcagt caaggaaggg tcagggcgtg gtcaccgcgg
      961 ccgtcgaccg ggctcgaggg cgtggacggc gtcgcgaaac tggtcgccgc ggtcgaactc
     1021 gtcgacgaag agcccgaagc tggcgcagcc gggtgacaac aggacgaccg cgccgtcggg
     1081 tgccaggctc accgcggcct gcaccgcgtc gcgcatcgag tcgaagggtc ccgcggtggt
     1141 gtcccgagcg aggcgatcct ggaggccggg ggtggcggaa ccggcgagga gcacggtggc
     1201 cacggcctgg tcgttgaggg ccgcgacgag cgcgtcgagg tcgaggccct tgtcgaaccc
     1261 gccggcgatg gcgacgacgg ggcggccagg gaacgctcgc agcgcggcct cggcgtcttc
     1321 gggggtggtg gcggtggtgt cgttgacgaa gtcgacgccg gcgatcgtgg ccacgggctc
     1381 gagtcggtgg gggacgccgt cgaagcgggc caccccgcgg cgcacctcgt cggcggtcac
     1441 gccgacggcc agggccgcgc cggccgcggc gagcgcgttg cgccggttgt gggccccggg
     1501 gacccgcagc gcatcgaccg ggccgaggtc gaggtcgccg ccgtcccagg aggcgacgaa
     1561 gtgcccgtcg gcgacgaaca acgtccgggg gccgaccggg tcgtcgccga acgtgatcgg
     1621 cggggcgggc gtcccggaca cgagggcgtc gaccgcaggg tcgtcggccg gcacgacctt
     1681 ccagtcgtgg gtgtgctggt ggcgaacgat ctgggccttg gcgtcggcgt acgcgtcgaa
     1741 gctcgggtag cggtcgaggt ggtcaggccg aacgttggtg atcaccgcga cggtgggggc
     1801 caaggcgtgc tcaccgaggc cttcgagctg gaacgacgac agctcgagga tgaccggcac
     1861 gtccgggtcg acgtcgccga gccgggccag cgccgaacgg cccatgttgc cggcgacgac
     1921 ggtcccgggc cagcgttccc gcaggatcga gtgggtgagc atcgcggttg tcgtcttgcc
     1981 cttcgtaccg gtgacgccaa tcgtcggcgc ggcacacgcg gcgaggaaca aggtcatctc
     2041 catctcgatg cgggccccgt gctcctcggc gactcggagg tacggggagt cccgacgaac
     2101 gtcggggttt cggatcacga cgtcggccca gccgaagtcg acctcgtcgt ggtgccccag
     2161 gacgaactcg accggcgttc ccgcgaactg ctcgacgatg tcgtggaacg acgcggggtc
     2221 gccgcgatcg gtgacgcgca cctgcgcgcc cgattcgacg gcgtagcgga tggcgccggc
     2281 accgccggcg cgtcggccga tgcccatgat gagcacccgg gatccggcca ggctggccgg
     2341 cgtcacgccg agccgcccac tacgcgcttg ggggggtagt cgcgaaacgt gagcagatgg
     2401 tcttggatgc cgttgcgaaa cgagtcgatg tacgccagct tgcggatcag gtagctgcgc
     2461 gtctccttcg ggtccaggta gaggttgtag agccgcccgt aggggtagcg ctgggtcacc
     2521 ccggtgaacc cgccggggtt gagcacgtcg tgatcgtcgt cggagatcga ttcgagcatg
     2581 aacttgtact cgccgacccg caccgccgag aaccggtcca gcagccagta gtagtggtac
     2641 ttgcggttcg acggtgcccc gggggcgagc aggaacgacg tctggtcgac cccgtcgatg
     2701 aaccggtcgg tgggtaccga gtcggcgcag ccggcgagcg tcacgacggt cgggagcacg
     2761 tcggtgaacg cgaacagccc gtcggaggcc tgtccggcgt cgacgacgcc gggccagctg
     2821 aacaacgcgg ggactcgtac cccgccctcc caggttgacc ccttcgcgct tcggaacggc
     2881 gagtacgcgg cgtcgggcca ggtctccatc tcggggccgt tgtcggagct cacgaacacg
     2941 aacgtgtcct cgagctggcc ggtggtccgc agcgtctcga ggagccggcc cacgatgtcg
     3001 tcgagctcga cgatggtgtc cttgtagggg tgacgggccg gggacacgcc gaggaaccgc
     3061 tggtgcgggt agttgtcgaa atgggcgccg cgggtgcagt ggtagaggaa ccacggggag
     3121 ccgccacccg ccatccgctc gaggaatccg atcgagtagt cgcaccaccg gtcgtcgagc
     3181 tgggagagca ctgggatcgt gacctcttcg acgttctcga cgtcggcccc gcgggtggcg
     3241 tgcacgaagc accggttgaa cggctggttc ttgatccact cggtgcgctc atcgctgtag
     3301 acgatctcgg ggaagaagta cgggtcgcgc cactcggtgt acatatcgga gacggagagg
     3361 aagccgtaga agtcgtcgaa gccgacgtgc tggggctgcg acgcggcgtt ctcgcccatg
     3421 tgccacttcc cgaccgcctg ggtcacgtac ccggcggagc tgaggagctc ggcgagggtc
     3481 acctcgccct caaggccacc gggctccccg tacatcggcg gacggagcag accgtggcgc
     3541 atcgggagcc gcccggtcat gagcgatgcc cgtgaggggc tgcacgacgg ctccgagtag
     3601 cacgacgtca gcagcagccc gtcgcgggcc agccggtcga ggttcggtgt cggcgcgccg
     3661 accgcgacgc cgccgccgta gcagccgaag tcaccccacc cgacgtcgtc gaacaagatg
     3721 acgagcacgt tggggttccg gccccgacgg gcccggtagt cgtcgaggcg ttgcctcgcc
     3781 gcgtcccggg cctcgtcgtg gacgaagtgg ggctcgcggt gtggggccac cgggacggtc
     3841 ggggtgacga tcggatcgaa ggtcaccgcg gcacgacgcg ccgcgctgtg ggttcgaggc
     3901 ccatgtcccg ccggcgcgct gccgcgccgt caccggctct gtccacgtct tctcgcaccc
     3961 cggactccct tcgcggcgcc attctgccct gcgcggtcga cgacggacga tttgcgtcgg
     4021 aggcgcggcg gcaactccgc cgcggccatg atggactcgg gagccgagat gaccgatgcc
     4081 agctgtgcga gttgcgggac gaccgacggg accgtgcgga tccgaccgtg gcacgaactc
     4141 gacgtggcgc tctgcgcgcg ctgctatgcc tcactgaccg gcgaccacga caaccacggc
     4201 gtcggcttca cgctcgcctg ggtggtgctc gtcaccgggc tgctcctcgt cgccgcggga
     4261 gccgcgacgt acctcctcac ccggtgatca cgccgcgctg atctgctcgc gcatccaacc
     4321 cgaggtgttg aagaagtcgg cgaccgcgct gcccgggtgg accagttcgt cgcaggcgac
     4381 gcgggcgtca tcgtccaacg accggtcgag gatcccgagg gcctcctcga gctgcgccag
     4441 ggtgcgcggc cccacgatcg gtgcggttac gtcaggctgg tccttgaccc agagcagtgc
     4501 tagctgtgag ctggtcaacc cccggtcggc ggcgagcgcc gcgacaccgc gagcgacctc
     4561 gagggcccgg gcggtgaccc ggtcccggag ctgcggtcgc cgtgccgcgc gcgagtcgtc
     4621 ggggatctgg tcgacgtcct ggtagcggcc ggcgaggatc ccacccccga gcggcgacca
     4681 cgggaggact ccgaggccct gcgctcggca gagcggcagg agctcgttct cgatgcgccg
     4741 gtcgagcagg ttgtaggggg gttgctccgt cgcgtacccg gggcgtccgt cgcgggccgc
     4801 gatcgctaac gcctccatca ccatccacgc cggatgcgtc gagcagccga cgtggcggac
     4861 cttgcccgcc tgcacgagct cgtcgaatgc ggcgagggtc tcggcttggg ggacggccat
     4921 cgacgggcgg tggagctggt agaggtcgat gtgatcggtc tggaggctcc gcagggacgt
     4981 ctcgcacgag tcgatgatgt gctcgcggcg gtgccaggtt ccgggagcgg ccttcccgcg
     5041 gggcatgccg actttggtcg cgaggacgac gtcgtcgcgc cggccgttgg ccgtgagcgc
     5101 gtcgccgacg atctgctcgc tggtgccgcc ggcgtacacg tcggcggtgt cgatcatcgt
     5161 cacgccagcg tcgagggcgg cgtcaatgat ccgggccgac tcggcgggtt cggtggggtc
     5221 accgaagttc atggcgccga ggcacagcgg cgcgacgcga agtccggtcg gtccgagggg
     5281 acgcagctcc atcattcagc cccgcgacca ttggtgagcg ttcatgtcgg gccccgggtg
     5341 catgacctgg gagtctggca ggtcatggcg gggagccccg gaggtgggaa tgcacccacc
     5401 tcaacggggc gaccccaccg ctcgcctggc gactacgccc gcagcgggtg gggcggggct
     5461 gggtttcgct gcagaccacc aggcgagttc ggcggcgtgc gagcacgccg atctcgcgtc
     5521 cttgcatctt tactaccact ggcggacgca cgacattccc gggccccgcg cacgccccaa
     5581 ctcgggcgaa accgggtggg gtcggttgcc gcgaatgccg cggaccgatc caaacccggc
     5641 gggcccgcgg tgcgg
//
LOCUS       DATCCH010000145         2403 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_215155, whole genome shotgun sequence.
ACCESSION   DATCCH010000145 DATCCH010000000
VERSION     DATCCH010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215155"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..120)
                     /locus_tag="VIJ44_02960"
     CDS             complement(<1..120)
                     /locus_tag="VIJ44_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEY5164892.1"
                     /translation="MSELAARVRDGDRRALARAITLVESTRPDHRSEADALLTD"
     gene            complement(161..2305)
                     /gene="scpA"
                     /locus_tag="VIJ44_02965"
                     /pseudo
     CDS             complement(161..2305)
                     /gene="scpA"
                     /locus_tag="VIJ44_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977581.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
BASE COUNT          348 a          764 c          871 g          420 t
ORIGIN      
        1 gtcggtgagc agcgcatcgg cctccgagcg gtggtcgggg cgggtgctct ccacgagggt
       61 gatggcgcgc gcgagggctc gtcggtcgcc gtcgcgcacg cgggcggcca gctcgctcac
      121 gcggccccgg cgatcggccg cggtcgcgcc gagcccgccg tcatccggcg tcagcgcggc
      181 ggagcagtcg gagcacttcg gacgcggccg ccgggacatt ggtgccggga ccgaacaccg
      241 cggcgacacc ggcgtcatga aggaactcgt agtcctggga tgggacgacc ccgccgacca
      301 ccacgacgat gtcgccacct ccttgcttac ggagctgggc gaccagctcg ggcacgaggg
      361 tcttgtgtcc cgcggcttgg ctgctcacgc cgatcacgtg cacgtcgttc tcgacggcgt
      421 cgcgcgccgc ctcctcgggg gtctggaaga gcggcccgac gtcgacgtcg aatccgaggt
      481 cggcgaacgc ggtggcgatg accttggcgc cacggtcatg accgtcttgg cccatcttga
      541 cgacgaggat ccgggggcgg cgcccctcgc cagccgcgaa cgcttcgacc tcggctcggg
      601 tgcgttccca ggcttcgtcg ccggctgcgg aatcgccgta cacgcccgcg atcgtacgga
      661 tcgtggcgcg gtggcgggtg aacactgact cgagggcgtc ggagatctca cccactgttg
      721 cccgggcccg gctggcttcg atgctgagcg ccaacaggtt ggcgtcaccg cgagcacctt
      781 cggtgagggc acgcaacgcc gcctccaccc ggctctggtc acgagtggcg cgcagctgct
      841 cgaggcgccg gatctggccg gaacgcacgg ccgtgttgtc cacctcacgg atctcgaccg
      901 tgggctgcgt gtccgtccgg tacttgttca cgccgacgat gacctcggcg ccgcggtcga
      961 cgcgggcttg gcgccgcgcc gcggcctctt cgatccggag cttgggcatg ccggcctgga
     1021 cggctcgggt catgccgccg agaccttcga cctcttcgat gagttcccag gccgcgttgg
     1081 cgagcgcgcc ggtcagcacc tcgacgtagt acgagccgcc aagcgggtcg acgacgtggt
     1141 tgatcccgct ctcttcttgc aacaccagct gggtgtttcg ggcgatgccg gcggagaact
     1201 cggtcggcag cgcgagggcc tcgtcaaagc tgttggtgtg gaggctctgc gtgccgccca
     1261 gcacggccgc gagcgcctcc agcgcggtgc gaacgatgtt gttgtacggg tcctattcgg
     1321 tgagcgagac gccggaggtc tggcaatggg tccgcagcat cagcgatgcc ggcttgttgg
     1381 gttcgaattg gcgcatgatc cgggaccaga ggagccgggc ggcccggagc ttggcgatct
     1441 ccatgaagaa gttcatgccg atcgcgaaga agaacgacag ccggccggcg aaggcgtcga
     1501 cgtcgagccc acggtcgagg gcggagcgga cgtactcgag tccatcggcg atggtgaatg
     1561 ccagctcctg gaccgcggtc gcgccagctt cttgcatgtg atagccggag atagagatcg
     1621 agttgaaccg gggcatgtgt tgtgctgtgt gctcgatgat gtcggcgacg atccgcatgc
     1681 tcggttcggg cggatagatg taggtgttgc ggaccatgaa ctccttgagg atgtcgttct
     1741 ggatggtccc ggcgagttgg gcttgatcga cgccttgttc ttcggcggcc acgatgtagc
     1801 cggcgagcac ggggagcacc gccccgttca tggtcatcga gaccgtcatg tgatcgagcg
     1861 ggatgccgtc gaagaggatc ttcatgtcct caacggagtc gatggccacg ccggcttttc
     1921 cgacatcgcc gaccactcgg gggtgatcgc tgtcgtagcc acgatgggtc gcgagatcga
     1981 aggccacgga gaggcccatc tgtccgtcct cgaggtttcg ccggtagaag gcgttggatt
     2041 cttccgcggt cgagaagccc gcgtactggc ggatcgtcca cgggcggttg gcgtacatcg
     2101 tcgcccggat cccccggacg aacggctcga atcccggcag agtgtcgacg gactcgagtc
     2161 cttcgaggtc ctcggcggtg tacagaggat tgaccgggat gccctcgggg gtcggccaga
     2221 tgagctgctc cgggtcgcct ttcacctcgg aggcggccag cgcctcccac tcggctcggc
     2281 tgtgctcggg gtcgtgctca cccatggtca gcgagggtag aggccgtctc ctcgatcggg
     2341 cgaccgatcg aggagacggt gggccttcgc cgcggcgggc tcggcgcagc ctggggcggg
     2401 gac
//
LOCUS       DATCCH010000146         4093 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_472874, whole genome shotgun sequence.
ACCESSION   DATCCH010000146 DATCCH010000000
VERSION     DATCCH010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472874"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1237
                     /locus_tag="VIJ44_02970"
     CDS             <1..1237
                     /locus_tag="VIJ44_02970"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY5164893.1"
                     /translation="YQHEAGDRVVITDAAGRQLASSEPRAEVDRGPADVLGPPEIARA
                     LGGHETSGSRYSRALGSDLWFVAVPVKSGSRVTGVVRVTHPLSSVAHRVRDNWLLLTA
                     VGGGVLAAVLVVSLLVALSVTRPLAELARAASRLGRGALHARAPVPSGPAEVRLLAQE
                     FNVTAEQLEALVGAQQSFVADASHQLRTPLAALRLRLENLESEVDPVAAEDVEGARTE
                     VERLTRLVDGLLALARAERAPSAPTNVDVDEVIAGRRDAWLAFAAERDVGLETQVPAR
                     LWAQVTPERLEQVLDNLLNNALELAPAGSVVTISGSHRRDRVEVRVRDRGPGMPAEDR
                     ARAFDRFWRGAGAPSDSGGFGLGLAIVRQLVAADGGTVELRSPPSGGLEAVISLRAGL
                     GPAVSMSPATEISGPAGPY"
     gene            1398..1643
                     /locus_tag="VIJ44_02975"
     CDS             1398..1643
                     /locus_tag="VIJ44_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164894.1"
                     /translation="MGGAVALVGLLSAMVSQALPGASGTTTSSQQPATAASPAAAPTS
                     PAPTAAPSSSSSSDTSLQPAPPPVVTHQRSVARSGGS"
     gene            1640..2569
                     /locus_tag="VIJ44_02980"
     CDS             1640..2569
                     /locus_tag="VIJ44_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEY5164895.1"
                     /translation="MTGPWASTSFPALGTTATLAVTHPAAFDPAHVELKALLAAVDKA
                     CSRFRSDSDLIHVNSGAGHPVKVGSMLLDALDVALRAARLTAGDVDPTVGRAMRVLGY
                     DRDFTEVVATGAPLTCVVERIPGWHTIQVDRTASTVQVPTGVELDLGATAKAFAADRA
                     ATTIADVIDGGILVSLGGDMAVAGTPPPDGWPVRVTDDHSTGPDAPGETVAIKAGGLA
                     TSSTAVRRWRRGDEQLHHIIDPSSGRPAPSPWRTVSVVAETCVDANIASTAAIVRGET
                     APSWLDELRLAARLVRRDGEAVHTGGWPAVVPS"
     gene            2566..3726
                     /locus_tag="VIJ44_02985"
     CDS             2566..3726
                     /locus_tag="VIJ44_02985"
                     /inference="COORDINATES: protein motif:HMM:NF013918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEY5164896.1"
                     /translation="MSVLATAGPSALWYLARGAGLAALVVLTLNMVLGIVTSVRWTNP
                     RWPRFVIELLHRNSSLVAFALIVVHVATVVIDSFAPIGWKDAFIPFVGVYRPIWLGLG
                     ALAFDILLALIITSLLRHRMSHRTWRFVHWFAYLCWPLVVVHGLGTGSDSKVGFVLLL
                     YVGCIAAVIMAAWWRLAVGWPNRVGVRIGALTTSVVAPFVLAAWLAAGPLAAGWARRA
                     GTPARVLAKVASTPAPASTPAPTVPPTAPASGSSALPAPPFSAQIVGVITQSNPSADG
                     RVTVRLANTLSGGATGVLNIDLTGQPVDGGGVALDTSQVTFGPSQQPSLYQGTVVALQ
                     GGRIVADLQGAGQPNLELILTVQTDRSGTKLSGSLRAQTTITGGNNPSGDGQ"
     gene            3723..>4093
                     /locus_tag="VIJ44_02990"
     CDS             3723..>4093
                     /locus_tag="VIJ44_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018771639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting membrane transporter"
                     /protein_id="HEY5164897.1"
                     /translation="MTTAATRPAPTPRHGPTGLPRLLAGYRPDGSMGLDEHLHTYGPP
                     PWRGTSRRGGTLINSIEAAGLRGRGGAAFPTGRKMRTVAQHRGRPVVVANGSEGEALS
                     AKDRLLLSNLPHLVIDGAALAA"
BASE COUNT          544 a         1507 c         1430 g          612 t
ORIGIN      
        1 ataccagcac gaggccggcg atcgcgtcgt catcaccgac gccgccgggc ggcagctggc
       61 gagctccgag ccccgcgctg aggttgaccg tgggccggct gacgtgctcg gtccacccga
      121 gatcgcgcgg gccttgggtg gccatgagac gagcggtagc cgctactccc gtgccctcgg
      181 cagcgacctc tggttcgtcg cggtgccggt caagtcgggg agccgagtga ccggggtggt
      241 acgcgtcacg caccccttgt catcagtcgc gcaccgggta cgcgacaact ggttgctcct
      301 gaccgcggtc ggcggcgggg tgctcgccgc ggtgctggtc gtcagtctcc tcgtggctct
      361 ctccgtcacc cggccgctgg ccgagctggc acgggccgcg tcgcgtctgg gccgaggcgc
      421 actgcatgcc cgcgcccccg tgccttctgg acccgccgaa gtgcgacttc tggcccagga
      481 gttcaacgtc accgccgagc agctcgaggc actcgtgggc gctcagcagt cgttcgtcgc
      541 cgacgcctca catcaactgc gaacgccgtt ggcggcgttg cggctgcggc tcgagaacct
      601 tgagagcgag gtcgatccgg tcgcggccga agacgtggaa ggggctcgca ccgaagtcga
      661 gcgcctaacg cgcctcgtcg atgggctgct ggcgctggcc cgcgccgagc gggcaccctc
      721 ggcgccgacc aacgtcgacg tcgacgaggt catcgccggg cgccgggacg cctggctcgc
      781 cttcgccgct gagcgcgacg tgggcctcga aacgcaggtc ccggcccggc tgtgggcaca
      841 agtgacgccc gagcgcctcg agcaggtgct ggacaacctg ttgaacaacg ccctggagct
      901 cgcaccggcg ggcagcgtgg tgacgatcag cggctcccac cggcgggatc gagtcgaggt
      961 gcgggtgcga gaccgcggtc cgggaatgcc cgccgaggat cgcgcccgcg ccttcgaccg
     1021 gttctggcgt ggcgccggag cgccaagcga cagcggcggg ttcgggctgg gcttggccat
     1081 cgtgcggcag ctcgttgccg ccgacggcgg caccgtcgag ctccggtccc cgccgtcggg
     1141 cggtctcgag gcggtgatct cgttgcgggc cggcctgggg cccgcggtct cgatgagccc
     1201 cgcgaccgag atctcagggc ccgcgggccc gtattagcca tttctttgct ctttgctgga
     1261 cggccggagg atcgacatct ggaagcctga ggtctgcacc gccccgttct ctacccccgt
     1321 ggagggtcac tatgcggttt cgaaccactg accgagatgt cgggctccgt cgcgtctcga
     1381 gcctgacccg ctgggtggtg ggtggcgccg tcgctctcgt cggcctgctc tccgcgatgg
     1441 tgagccaggc gctcccgggc gcctcgggga ccacgacgag ctctcaacag ccggcgacgg
     1501 ccgcgtcgcc cgccgcggca ccgaccagcc cggcgccgac cgccgcaccc agcagcagca
     1561 gcagcagcga caccagcctc cagcccgccc cgcccccggt cgtgacccat caacgcagcg
     1621 tggcgcgctc cggcgggtcg tgaccggacc ctgggcctcc acaagcttcc ccgcgctggg
     1681 gacgacggcg accctggcgg tcacccaccc cgcggccttc gacccagcgc acgtcgagct
     1741 gaaggccctg ctcgcggcgg tggacaaagc atgcagccgt ttccgctccg actcggacct
     1801 gatccacgtc aacagcgggg ccggacaccc ggtgaaggtc ggatccatgc tcctcgacgc
     1861 actcgatgtg gctcttcgcg ccgctcgcct caccgccggt gatgtcgatc cgacggtggg
     1921 ccgcgccatg cgggtcctcg gctacgaccg cgacttcacc gaggtcgtgg cgacgggggc
     1981 gccgctgacc tgcgtcgtcg agcggatccc cggctggcac acgattcagg tggaccgcac
     2041 cgcgtcgacc gtccaggttc ccaccggcgt cgagctcgac ctgggcgcca cggcgaaagc
     2101 cttcgccgcc gaccgcgccg cgacgacgat cgccgacgtc atcgacggcg ggatcctggt
     2161 gagcctcggc ggcgacatgg cggtcgctgg caccccgccc ccggacggtt ggcccgtgcg
     2221 ggtcacggac gaccactcga cgggacccga cgcgcccgga gagacggtcg cgatcaaggc
     2281 gggcgggctc gccacctcga gcaccgcggt gcgccgctgg cggcgcggcg acgagcagct
     2341 gcaccacatc atcgacccgt cctctggtcg gccggcaccg agcccctggc gaacggtgag
     2401 tgttgtcgcc gagacctgcg tggacgccaa catcgccagc accgcggcga tcgtgcgggg
     2461 cgagacggcg ccaagctggc tcgacgagct caggttggca gcccggctcg tacgtcgcga
     2521 cggcgaggcg gtgcacaccg gcggctggcc cgcggtcgtc ccgtcatgag cgtgctcgcg
     2581 acggctggtc cgtcggcgct ctggtatctc gcccggggcg ctggcctcgc cgcgctggtg
     2641 gtcctgacgc tgaacatggt gctcggcatc gtgacctcag tccgctggac caacccgcgc
     2701 tggccacgtt tcgtcatcga gctgctgcac cgcaactcgt cgctggtcgc gttcgcgctg
     2761 atcgtcgtgc acgtcgccac cgtcgtcatc gactcgttcg cgccaatcgg ctggaaggac
     2821 gcgttcatcc cgttcgtagg ggtctaccgg ccaatctggc tcggcctcgg cgcgctggcc
     2881 ttcgacatac tcctggcgct gatcatcacc agcctgctgc gtcaccggat gagccaccgg
     2941 acctggcggt ttgtgcactg gttcgcgtac ctgtgctggc cgctcgtcgt ggtgcacggg
     3001 cttggtactg gcagtgactc caaagtcggg ttcgtgctgt tgctctacgt cggatgcatc
     3061 gcggcggtga tcatggcggc ctggtggcgt ctcgcggtcg gctggcccaa ccgggtcggt
     3121 gtgcgaatcg gcgccctgac caccagcgtc gtcgcgccgt tcgtcctcgc ggcgtggctc
     3181 gccgcggggc cgctcgccgc cggctgggct cgacgggctg ggacgccggc gcgcgtcttg
     3241 gcgaaagtgg cctcgacgcc cgcccccgcc agcaccccgg ccccgaccgt gccgcccact
     3301 gcgccggcct cggggtccag cgcgcttccc gcgcctccct tcagtgcgca gattgtcggc
     3361 gtgattaccc agtccaaccc gtcggccgac ggtcgggtga ccgtgcggct ggccaacacc
     3421 ctcagcgggg gcgcgaccgg cgtgctcaac atcgacctca ccgggcagcc tgtggacggc
     3481 ggtggagtgg ccctggacac cagccaagtg acgttcgggc cgtcccagca acccagcctg
     3541 tatcaaggca cggtcgtcgc gttgcagggc gggcgcatcg tggccgacct gcaaggggcc
     3601 gggcaaccga acctggagct gatcctgacg gtgcagaccg accggtccgg gaccaagttg
     3661 agcggaagcc tgcgcgcgca gaccaccatt accgggggca acaatccgtc tggggatggc
     3721 caatgaccac tgccgccact cgacccgcgc cgacgccgcg ccacggtccg accggcctgc
     3781 cccggcttct cgcgggatac cgccccgacg ggtccatggg cctcgatgag cacttgcaca
     3841 cctacgggcc gccaccatgg cgcggcacaa gccgccgcgg cggcaccctc atcaactcga
     3901 tcgaagccgc cggtctgcgc ggccggggcg gcgcggcctt cccgaccggg cgcaagatgc
     3961 ggaccgtcgc ccagcaccgg ggccgcccgg ttgtggtcgc gaacgggtcc gaaggggaag
     4021 cactcagcgc caaagaccgg ctgctgctca gcaacctgcc ccacctcgtg atcgacggcg
     4081 ctgcgctcgc cgc
//
LOCUS       DATCCH010000147         3252 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_306273, whole genome shotgun sequence.
ACCESSION   DATCCH010000147 DATCCH010000000
VERSION     DATCCH010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306273"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..908)
                     /locus_tag="VIJ44_02995"
     CDS             complement(12..908)
                     /locus_tag="VIJ44_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164898.1"
                     /translation="MWWRRRDGDLVRDLPRNRRVMPYLMRGRNESAVYFEHDIPLRKS
                     DAFIREWNQANPGLRIDVFHLAVWGLRQTTERNPTINRFIAGGRLYDRRGIWFSYAVK
                     SKLVTGAPLIVVKRRMDLDESFAAMVVGMHETEAQYRSGQADRVDQELGLLLFFPGFA
                     RRIIMRGVNGLDRLGMLPRSYLEGDPMYSSAFLANMASLGMPPVYHHLYEYGTASIFC
                     SLGRPIPEPGSPTSGPDRRRTMQVRWTFDERAEDGLTAWFAARRFRQVVEDPIACGVI
                     IEAVAPDATHPIGEVVDDAARF"
     gene            complement(920..1807)
                     /locus_tag="VIJ44_03000"
     CDS             complement(920..1807)
                     /locus_tag="VIJ44_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019730658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY5164899.1"
                     /translation="MRHPGLALLTLAGVLAACSGGPSTSVGTSQHSAGPSTSTSTTTT
                     TIAVRADSPAALADTLTQIERGLRQPTAASTPSLGRAQQSAYGALKAHPDWRPTVLAA
                     IGSDVHDAVNTNVDADLALEALFTGSGPAPTSLPITSIRIPVPAAQLLADYQEAAVTT
                     GVPFAYLAAINFIESRFGRNPGQSTAGAQGPMQFEPGTWAQYGHGGNIQDDHDAILAA
                     GRLLAANGGPANIAKAIFAYNNSHEYVRAVQDYANVMIAAPSAFYGYYQWQVYTKTTA
                     GTFLLPEGYPNQPAVRTSG"
     gene            1847..2425
                     /locus_tag="VIJ44_03005"
     CDS             1847..2425
                     /locus_tag="VIJ44_03005"
                     /inference="COORDINATES: protein motif:HMM:NF017697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate hydrolase family protein"
                     /protein_id="HEY5164900.1"
                     /translation="MFAELLACPGVEQHVTLRSGVGFLALHGGLEPGTAELARSAADR
                     SRASVYTVVQPGDLRWHVPAHRVEPGECPSLAEFLAHVAVVVSLHGYRRADLPMALLV
                     GGSNRPLAARLGTQLRAGLPDYDVIDDLARIPPDLRGVHPHNPVNLTRAGGIQLELPH
                     PVRSIGPYRDASYQAHTVALVDILAGFARSEA"
     gene            2433..3128
                     /locus_tag="VIJ44_03010"
     CDS             2433..3128
                     /locus_tag="VIJ44_03010"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTAP family purine nucleoside phosphorylase"
                     /protein_id="HEY5164901.1"
                     /translation="MGRLAVIGKEGTLGSPAASARVALPQPIGVLDHGTHVTLYRHGL
                     DHYVAPHRIDHAAHLRTLVQLGCDRVLALSSVGSLRVDLPVGSFVAPHDFVALDQHPV
                     DVNDADAQHVVPGFTDGWRHQILTVWRRVAGAPIVDRGIYWQANGPRFETPAEIRYIA
                     TFADVVGMTVPSECVAANQLGVAYAAVCIVDNLANGVADTPLTTEEFERGAAANVARL
                     TAALSHVIPELAA"
BASE COUNT          442 a         1167 c         1124 g          519 t
ORIGIN      
        1 tgcgccccag cctagaagcg ggcagcgtcg tcgacgacct cgccgatggg gtgggtggcg
       61 tcgggtgcga cggcctcgat gatgaccccg cacgcaattg ggtcctcgac gacctggcgg
      121 aaccgtcgcg ccgcgaacca ggcggtcaac ccgtcctcgg ctcgctcgtc gaacgtccat
      181 cggacctgca tcgtgcgtcg ccgatcgggg ccgctcgtcg ggctgccggg ctcggggatg
      241 ggacgtccga gcgagcagaa gatgctcgcc gtcccgtact cgtagaggtg gtggtacacc
      301 ggcggcatcc cgaggctggc catgttggcc aggaacgcgc tcgagtacat cgggtcacct
      361 tcgaggtatg agcggggcag catcccgagc cggtcgaggc cgttcacgcc tcgcatgatg
      421 atccggcggg cgaacccggg gaagaagagg agcaacccga gctcctggtc gacccggtcc
      481 gcttggccgc tgcggtactg ggcctcggtc tcgtgcatgc cgacgaccat cgccgcgaac
      541 gactcgtcga ggtccattcg gcgcttcacc acgatgagcg gtgccccggt gacgagcttg
      601 gacttgaccg cgtacgagaa ccagatcccg cgccggtcgt ataggcgccc gccggcgatg
      661 aaccggttga tggtcgggtt ccgttcggtg gtctggcgca agccccagac cgcgaggtgg
      721 aacacgtcga tgcgaaggcc cgggttcgcc tggttccatt ctcggatgaa cgcgtccgac
      781 ttgcgcaatg ggatgtcatg ctcgaagtag acggccgatt cgttgcggcc ccgcatgagg
      841 tacggcatga cccgccggtt gcggggcagg tcccggacga ggtcgccgtc tctccgtcgc
      901 caccacacgg ccgttgagcc tatcccgacg tccggacggc gggctggttc gggtagccct
      961 cgggcaacag gaacgtcccg gcggtggttt tggtgtacac ctgccactgg tagtagccgt
     1021 agaaggcgga tggggccgcg atcatcacgt tggcgtagtc ctggacggcg cgcacgtact
     1081 cgtggctgtt gttgtacgcg aagatcgctt tcgcgatgtt cgccggacca ccgttcgcgg
     1141 ccaggagccg gccggcggcg aggatcgcgt cgtggtcgtc ttggatgttg ccgccgtggc
     1201 cgtactgggc ccacgtcccg ggttcgaact gcatcggtcc ttgggctccg gcggtgctct
     1261 ggcctggatt gcggccgaag cgactttcga tgaagttgat cgcggcgagg tacgcgaacg
     1321 gcacgccggt ggtgaccgcg gcttcctggt agtcggcgag cagctgcgcc gccggcacgg
     1381 gaatccggat cgacgtgatc ggcagcgacg tcggggccgg accgctgccg gtgaagaggg
     1441 cctcgagggc caggtcggcg tcgacgttgg tgttcaccgc gtcgtgcacg tcggaaccga
     1501 tcgcggcgag gacggtcggc cgccaatccg ggtgcgcctt gagcgcgccg tacgccgact
     1561 gctgggctcg acccaggctc ggggtcgacg ccgcggtggg ctgacgcaac cctcgctcga
     1621 tctgggtcaa cgtgtcggcg agcgcggcgg gactgtcggc tcgcactgcg atcgtggtgg
     1681 ttgtggtgct ggtgctcgtc gacgggcccg ccgagtgttg ggaggtgccg acgctcgtgc
     1741 tcggcccgcc cgaacaggcc gcgagcactc cggcgagggt cagcagcgcg agacccgggt
     1801 gacgcatccc gtgaggatgg catgcggccg gtagcctgcg cgctcagtgt tcgccgagct
     1861 tctggcctgc cccggcgtgg agcagcacgt cactctccgc tccggggtgg ggttcctcgc
     1921 cctccacggc ggtctggagc ccggcaccgc cgagctggcc cggtccgccg ccgaccggag
     1981 ccgcgcctcg gtgtacaccg tcgtccagcc cggcgatctc cgctggcatg tcccggccca
     2041 tcgggtcgag ccgggcgagt gcccgtccct cgccgagttc ctcgcccacg tcgccgtcgt
     2101 ggtgtccctc cacggctacc ggcgtgccga tctgcccatg gccctcctcg tcggcggctc
     2161 gaaccgtccc ctcgccgccc ggctcggcac gcagctgcgc gccggcctcc ccgactacga
     2221 cgtgatcgac gacctggccc gcatcccccc cgacctgcgc ggcgtccatc cgcacaaccc
     2281 ggtgaacctc acccgcgccg gggggatcca gctcgagttg ccccacccgg tccggtcgat
     2341 cggcccgtac cgcgacgcgt cctaccaggc tcacaccgtc gcactggtcg acatcctcgc
     2401 cgggttcgcc cggtccgagg cgtagccgac cggtgggtcg cctcgccgtc atcgggaagg
     2461 aaggaaccct cggatcaccc gcggcgtcgg cgcgcgtcgc cctcccccag ccgatcggag
     2521 tgctcgacca cggcacccac gtgaccctgt accgccatgg tctcgaccac tacgtcgccc
     2581 cacaccgcat cgaccacgcc gcgcacctcc gcactctcgt gcagctcggc tgcgaccggg
     2641 tcctggcgct cagctcggtc gggtcgttgc gggtcgacct gcccgtcggc agtttcgtcg
     2701 ccccgcatga tttcgtcgcg ctcgaccagc accccgtcga cgtcaacgac gccgacgcgc
     2761 agcatgtcgt ccccggcttc actgacgggt ggcgtcacca gatcctgacc gtgtggcgac
     2821 gggtcgcagg cgcgccgatc gtcgaccgtg gcatctactg gcaggcgaac ggcccccgct
     2881 tcgagacccc agccgagatc cgctacatcg cgacgttcgc cgacgtggtc ggcatgacgg
     2941 tcccgtcgga gtgcgtcgcc gcgaaccagc tcggcgtggc gtacgcggcc gtctgcatcg
     3001 tcgacaacct tgccaacggg gtcgccgaca cccccctcac caccgaggag ttcgagcgcg
     3061 gcgccgcggc gaacgtggcc cggctgaccg ccgcgctcag ccacgtcatc ccggagctcg
     3121 cggcatgagc ctcgcggtga ccggagccac cgcgctcgac ggcaccccag ccgcgctgcg
     3181 cgccgtcaac gggaggattg tcgacctcgg cgccgacgtc acccccggac ccgacgacga
     3241 gatcctcgac gc
//
LOCUS       DATCCH010000148         2576 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_381682, whole genome shotgun sequence.
ACCESSION   DATCCH010000148 DATCCH010000000
VERSION     DATCCH010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381682"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            659..1558
                     /locus_tag="VIJ44_03015"
     CDS             659..1558
                     /locus_tag="VIJ44_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164902.1"
                     /translation="MRDDEIGVRARGLGATLEPVAGQVYFSPECHAQYEALGFGASPG
                     LAGAVALPDGPAYLCSRGSVMGQVPGELVAAAFGVFNPAAVVPAVTYGWTLTDAATIC
                     EARTAGATAQLIRILGERPDGIKEATALLTEANEPLRPEGRPLYAGLLALGLPGNPMG
                     DLWRLADRLREYRGDAHTAAWTSAGYDAAEIGLLTELYWGLPPRTYVRTRAWSDADLD
                     AAEATLTERGLLTESALTDAGRTAREEIEHATDRQCRQIVEALGDRLDTLVSILGPWG
                     EAIRAAGGYLAAGPHDLATSAAS"
     gene            1632..2270
                     /locus_tag="VIJ44_03020"
     CDS             1632..2270
                     /locus_tag="VIJ44_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164903.1"
                     /translation="MTGVEVFVDPSCPWAWITSRWVKEVAPRRDLTVTWPSYCLEIRD
                     DYGVAPTVPESLRETVIAAHAISHRMLRIFEAARAEAGEDAVDALYTEWGRRFFAAGG
                     PRDDELLSQCLAATALDPDLVLAADDEKWDTPIIEAMEVAYAFGGPKTQTPTIVVGSD
                     RPHGFKGPVMAPAPTGDAAARLWDAIEVISQEPGFFEITRPRANPPRPPALD"
     gene            complement(2309..>2576)
                     /locus_tag="VIJ44_03025"
     CDS             complement(2309..>2576)
                     /locus_tag="VIJ44_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase S12"
                     /protein_id="HEY5164904.1"
                     /translation="VAGNSEPPGPGVNAAGLGIFRYQTSCGTVYGHTGNTAGYTQFIA
                     ATRDGSRSATVSVNAQVNPRTDAKLFPTLRRVFSTAVCAALART"
BASE COUNT          381 a          894 c          898 g          403 t
ORIGIN      
        1 cccgggcggc gtcacccgga tggcaccgga gcgtgactgg gccgtgacag tccgtggtcg
       61 ttgatctgag tcggggcggc ggtgaggacg atgtggagcc aaagcctcga ctgctcggcc
      121 acggtgtccg ggttgcacga gggtcaggga gctgtggacg ccggtgggag gtcatcggtc
      181 tccgcggatc tgggctcgta gccgctcggc cgatccgtcc tcgacccagg cccgaatgat
      241 caacagcccg gttcgacggg tgaccatttc ctcaccggcg gaacggtccc atgccccggc
      301 cgtgatggcc gtggagcgca gcagtgagtt cacaccgtcc ccccgggcgg ttggctcccg
      361 cggcgagccg ctatgtgccc cacggcgggc ccaggcaaag ccaagacgac cagctcgcag
      421 ccgcgacacc gagagcatcg agaactcggg catcccggaa gcgcacggtc gctccgacgg
      481 ctcgatcggc ggatagctca accacggacc gcggctcggc cgagcgggca tgggtgggaa
      541 gagttgcagt tcacgtcggc agcggacgac tggtcgcgct tcgccctcat cagcggctct
      601 gccccttcgc tcacctgtcg gtgtcgggga tctcgggcgg gccgtaccat gaccagcgat
      661 gcgagacgac gaaatcggtg ttcgggcccg agggttgggc gcgacgctcg agccggttgc
      721 cggccaggtc tatttctccc ccgaatgcca cgcgcagtac gaggcgctcg ggttcggggc
      781 cagccccggc ctggccggtg cggtggcgct acccgacggg ccggcgtacc tctgtagtcg
      841 gggttcggtc atgggccagg tgcctggcga gctggttgcc gccgcgttcg gggtgttcaa
      901 ccccgccgcg gtggtgcccg ccgttaccta cggctggacc ctgaccgacg cggccacgat
      961 ctgcgaagcg cgcaccgccg gcgccaccgc gcagctcatc cgaatcttgg gtgagcgccc
     1021 cgacgggatc aaggaggcga ccgcgctgct gaccgaggcc aatgagccgc tccgccccga
     1081 aggccgacct ctctacgccg ggctgctcgc cctcgggttg ccgggcaatc cgatggggga
     1141 cttgtggcgc ctcgccgacc ggctgcgcga gtaccgcggt gacgcacaca ccgcggcgtg
     1201 gacctcagcg ggctacgacg ccgccgagat cggactgctc accgagctct actggggatt
     1261 gccgccccgc acctacgtgc gcacccgggc ctggagcgac gctgacctgg acgccgccga
     1321 ggccacgctc accgagcggg gcctcctcac cgagagcgct ctcaccgacg ccggccgtac
     1381 cgcgcgcgag gagatcgagc acgccaccga tcgtcaatgc cgtcagatcg tcgaggccct
     1441 tggcgacagg cttgacaccc tcgtaagcat tctcggtccg tggggcgagg ccatccgcgc
     1501 cgcgggcggt tatctcgccg cgggcccgca cgacctggcc accagcgccg cctcctaagc
     1561 gctctcgagc cccgggtggc gatggtgtcc tggcccgcgc tcggcttcga acccgtcgat
     1621 agcctcgcgt catgacgggc gtcgaggtgt ttgtcgatcc gagttgcccc tgggcgtgga
     1681 tcacgtcccg gtgggtcaag gaggtcgcgc cgcggcgcga tctgaccgtg acgtggcctt
     1741 cgtattgcct cgagatccga gacgactacg gcgtggcccc cacggtgccg gagagccttc
     1801 gggagacggt gatcgccgcc catgccatct cgcaccggat gctccgaatc ttcgaggcgg
     1861 cccgagctga ggctggagag gacgcagtcg acgcgctcta caccgaatgg ggtcgccggt
     1921 tcttcgctgc tggtgggcca cgcgacgacg agctcttgtc gcaatgtctt gccgcgaccg
     1981 cacttgatcc cgatcttgtc ctcgcggccg acgacgagaa atgggacacg ccgatcatcg
     2041 aggcgatgga ggtcgcctac gcgtttggtg gtcccaagac gcagaccccg acgatcgtcg
     2101 tgggttccga ccgacctcat gggttcaagg ggccggtgat ggcgccagcc cccaccggtg
     2161 acgccgcagc gcgactctgg gatgccatcg aggtcatctc acaagagcct ggcttcttcg
     2221 agatcacccg gccccgcgcc aacccgccgc ggccacccgc cctcgactga cgtcaccggg
     2281 ccggccgatt ccgtgacggc cagtggggct aggtgcgagc gagcgcggcg cacaccgcgg
     2341 tgctgaagac acgccgcagc gtcgggaaga gcttcgcatc ggtcctcggg ttgacctggg
     2401 cgttcacgga cacggtcgcc gaccgagaac cgtcacgcgt cgcggcgatg aattgcgtgt
     2461 agcccgccgt gttcccggta tggccataga ccgtgccgca tgacgtctgg tatcggaaga
     2521 ttcccagccc ggcggcgttg acaccgggtc ccggcggttc ggagttgccc gcgacg
//
LOCUS       DATCCH010000149         4293 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_99664, whole genome shotgun sequence.
ACCESSION   DATCCH010000149 DATCCH010000000
VERSION     DATCCH010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4293
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99664"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VIJ44_03030"
     CDS             complement(<1..467)
                     /locus_tag="VIJ44_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010308774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HEY5164905.1"
                     /translation="MPEDPAALRLPDELIPADGRFGSGPSKVRDEAMARLFDSAHGYL
                     GTSHRRPGVKTVVHRAREGLRELYTLPDGYEILLGNGGSTAFWDAAAFGLIKRRSQHL
                     SFGEFSAKFAAVTASAPFLEDPEVIESPPGTHPDPRPNPDVDAYALTHNETSTG"
     gene            complement(534..1196)
                     /locus_tag="VIJ44_03035"
     CDS             complement(534..1196)
                     /locus_tag="VIJ44_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003989162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEY5164906.1"
                     /translation="MTPPPELARFDRVERAAHWSTASLFGVLMFTGAALYAGPISTLV
                     GQREVVRTIHVGAGLLLPFPLLVGLAGRWGRALRGDLHRLGRFDDEDRRWLRRHSRAD
                     ARLGKFNPGQKLNAAFLGSAIVVMLGTGIVLKWFSLFSLTTRTGATFVHDWVALGIWI
                     SVLGHIMLAVRDPIALRGMLHGAVTARWARTNRPRWYEAETGEDAGRLTPPDPRGPPR
                     RA"
     gene            complement(1180..1905)
                     /locus_tag="VIJ44_03040"
     CDS             complement(1180..1905)
                     /locus_tag="VIJ44_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007446220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY5164907.1"
                     /translation="MERAIRGDTSETAVGRRVFLGLLGFGAAGIVFGSSVQGWLERNV
                     GPVLAKDPTGLLALLPIGRFRIYSVTADLPDRSRSAYRLKVRGLVDRETVFTFDELAA
                     MPKTHLTRDFQCVTGWRVPEVRWSGVRLADVLDRAGVQADAGAVRFVSFDGTYTESLT
                     VTQARRADVLVAYELEGEALSSEHGGPARLYIAPMYGYKSLKWLQTIELTRHVEPGYW
                     EQRGYDVDGWVGRSNHRSDDPTA"
     gene            complement(1994..3157)
                     /locus_tag="VIJ44_03045"
     CDS             complement(1994..3157)
                     /locus_tag="VIJ44_03045"
                     /inference="COORDINATES: protein motif:HMM:NF016218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF459 domain-containing protein"
                     /protein_id="HEY5164908.1"
                     /translation="MAKTTRPPEPDPPPDGSLPPIAYEAEDGAPARRPLAAGHVIVVG
                     LVALLVGALLNAPGIRKTALGQPVGWKRDISTAFANPLYDVSHALYLDRLRQGLKDVL
                     GRSGDDSVNANLPSPTTLPAGPTPTTLPSRLAFSPSHPLRLWIGGDSLGDTPGSSLID
                     QLSSDRAVGILGPVDTHISTGLARPEVFNWPEFLKTVVTTDQPGAVVLTFGANDDQNL
                     TGDGGGESFGSPAWQAEYRRRIGGLMDVVTGTASHPKLFFVGIPPVRDMARFSNDYVL
                     INNIINSEAQARPGRVYYVDTVSALGNPTGGYADDLLNPDGTIVRVRSSDGIHFTRAG
                     GDRVAAKILAAMNQAFDLTSWFKDQSTTTTTTKPGTKARRPAAAATTTTVRKR"
     gene            complement(3159..>4293)
                     /locus_tag="VIJ44_03050"
     CDS             complement(3159..>4293)
                     /locus_tag="VIJ44_03050"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HEY5164909.1"
                     /translation="VRYSRDVLDGMGLSVGNTIIAVTLPVGISFFTFQALSYVIDIYR
                     GTFEPREFLEFAVYLSFFPHVISGPIVRAAEFLPQLRERHNARRVDASRAFFLIFLGL
                     FKKVVIANFLGTQIVDIVFANPKQHSSLEVVVSVYAYAIQIYADFSGYTDMAIGLALL
                     LGFRFPQNFDAPYTAVSLQDFWRRWHMTLSRWLRDYLYVPLGGNRNGRWRTYRNLMIT
                     FVLGGLWHGAAWTFVVWGAIHGGFLSVEHWRRTAHGKHGHRPDDTRRHRLIQRLVTFH
                     VVCFAWVFFRADSFKNAADVLGRIVDPSHWFHAAPLITGGVLLAIGAGLAEQYIPRDT
                     MARAMASFSRLSPVAQGVTLGFVLLVTNTMGPRGVAPFIYFRF"
BASE COUNT          699 a         1433 c         1536 g          625 t
ORIGIN      
        1 ccggtcgagg tctcgttgtg ggtgagcgcg taggcgtcga cgtcgggatt cgggcgcggg
       61 tcggggtgcg tccccggtgg ggactcgatc acctcgggat cctcgaggaa cggcgccgac
      121 gcggtgaccg cggcgaactt ggccgagaac tccccgaagc tcagatgctg gctgcgccgc
      181 ttgatcaacc cgaacgcggc cgcgtcccag aacgcggtgg agcccccgtt gccgagcagg
      241 atctcgtacc cgtccggcaa cgtgtacagc tcgcggaggc cttcgcgggc gcggtgcacg
      301 accgtcttga cccccgggcg gcgatggctg gtgccgagat acccgtgggc ggaatcgaag
      361 agccgggcca tggcctcgtc gcgcaccttg gacggcccgg atccgaagcg gccgtcggcg
      421 gggatgagct cgtcggggag acggagggcg gcgggatcct cgggcatcat cagcgaggct
      481 actggtggcc tcccggtgtc gccccgaagt tcctgggagc tcggccgcgc gggctacgcg
      541 cgccgcggcg ggccgcgcgg gtcgggcggc gtgagccggc ccgcgtcctc gccggtctcc
      601 gcctcgtacc aacggggccg gttggtgcgc gcccagcgcg ccgtcaccgc gccgtgcagc
      661 atcccgcgca gcgcgatcgg gtcgcgcacc gccaacatga tgtgcccgag gaccgagatc
      721 cagatcccga gcgcgaccca gtcgtgcacg aacgtcgcgc cggtccgggt cgtgagggag
      781 aacagcgaga accacttgag cacgatgccg gtgccgagca tcaccacgat cgccgaaccg
      841 aggaacgcgg cgttgagttt ctggcctggg ttgaacttcc ccagccgggc gtcggcgcgg
      901 ctgtggcgcc gaagccaccg tcggtcctcg tcgtcgaagc gtcccaatcg gtgcaggtca
      961 ccgcgcaggg cgcggcccca ccggccggcc agcccgacga gcagcgggaa cggcaacagg
     1021 agtcccgcgc cgacgtggat ggtgcgcacg acctcgcgtt ggccgaccag ggtcgagatc
     1081 ggaccggcgt agagagcggc accggtgaac atcagcaccc cgaacaacga cgccgtcgac
     1141 cagtgcgcgg cgcgctcgac ccggtcgaag cgtgccagct caggcggtgg ggtcatcgct
     1201 tcgatggttg gagcggccca cccagccgtc gacgtcgtag ccgcgctgct cccagtagcc
     1261 gggctcgacg tggcgggtca gctcgatggt ctggagccac ttcagcgact tgtagccgta
     1321 catcggcgcg atgtagagcc gggcgggccc gccgtgctcg ctcgaaaggg cctcaccctc
     1381 gagctcgtag gcgacgagca cgtcggcgcg gcgggcctgg gtcacggtga ggctctcggt
     1441 gtacgtgccg tcgaacgaca cgaaccgcac cgcgcccgcg tcggcctgga cgccagcacg
     1501 gtccaacacg tcggccaggc gcaccccgga ccagcggacc tccggcaccc gccagccggt
     1561 cacacactgg aagtcccggg tgagatgggt cttgggcatc gccgcgagct catcgaaggt
     1621 gaacacggtc tcccggtcga cgagcccccg gaccttcagg cggtacgccg atcggctccg
     1681 gtcgggcaga tcggcggtca cgctgtagat ccggaaccgc ccgatcggca gcagcgccag
     1741 gagccccgtc gggtccttgg cgaggaccgg cccgacgttg cgttcgagcc agccctggac
     1801 gctggagccg aacacgatgc ccgcggcccc gaaaccgagg agaccgagga acacccgccg
     1861 tcccaccgcg gtctcgctcg tgtcgcctcg aatcgctcgt tccacggatc tcgaccccgc
     1921 ctcgggcccg tcggccctca tgatgccagc gccatccccg cccactcctt cgggttctga
     1981 ccgtcgccgg gtctcagcgc ttgcggacgg tggtggtggt cgccgccgcg gccggccgac
     2041 gggccttggt cccgggcttg gtcgtggtgg tcgtcgtcga ctggtccttg aaccacgacg
     2101 tgaggtcgaa ggcctggttc atggcggcca ggatcttcgc cgcgacccgg tccccgccgg
     2161 cccgagtgaa atggatgccg tcgctgctgc ggacccggac gatggtccca tccgggttca
     2221 ggagatcgtc ggcgtagccg ccggtcgggt tgccgagcgc cgacacggtg tccacgtagt
     2281 agacgcgccc agggcgggcc tgggcctcgg agttgatgat gttgttgatc agcacgtagt
     2341 cgttggagaa tcgtgccatg tccctcaccg gtgggatgcc gacgaagaac agcttggggt
     2401 gactggcggt accggtgacc acgtccatca ggcccccgat gcgacgccgg tactcggcct
     2461 gccaggcagg acttccgaac gactcgccgc ctccgtcgcc ggtgaggttc tggtcgtcgt
     2521 tggccccgaa ggtgagcacc accgcgccgg gttggtcggt ggtcacgacg gtcttcagga
     2581 actccggcca gttgaacacc tcggggcgag ccagtccggt cgagatgtgg gtgtcgacgg
     2641 gaccgaggat cccgaccgcc cgatccgagg acagctgatc gatcagcgac gaccccggtg
     2701 tgtcgccgag cgaatcgccg ccgatccaga gtcgcagcgg atgcgacgga ctgaacgcca
     2761 gccgtgacgg caacgtggtc ggcgtcggac cggcgggcag cgtggtcggg ctcggcagat
     2821 tcgcgttgac actgtcgtcg ccgctgcggc ccagcacgtc cttcaacccc tgtcgcagcc
     2881 ggtccaggta cagggcgtgg ctcacgtcgt agagcgggtt cgcgaacgcg gtcgagatgt
     2941 cgcgtttcca gcccaccggc tggcccagcg cggtcttgcg aatcccgggg gcgttgagca
     3001 acgccccgac gagcaaggcc acgagaccga ccacgatcac gtgtcccgcg gccaacggtc
     3061 gccgggccgg cgcgccgtcc tcggcctcat aggcgatcgg cggcagcgag ccgtcgggag
     3121 gcgggtcggg ttcggggggt cgagtcgtct tcgccatgtc agaagcggaa gtagatgaac
     3181 ggcgccacac cgcgtgggcc catggtgttg gtgacgagga gcacgaagcc gagcgtcacc
     3241 ccttgagcga ctgggctcag acgggagaag ctggccatcg cacgcgccat ggtgtcacga
     3301 ggaatgtact gctcggccag accggcgccg atggcgagga ggaccccgcc ggtgatgagc
     3361 ggcgccgcgt ggaaccagtg cgatggatcg acgatgcggc cgaggacatc tgccgcgttc
     3421 ttgaacgaat cggcgcggaa gaacacccac gcgaaacaga cgacgtggaa ggtcaccagc
     3481 cgttgaatca ggcgatgccg ccgcgtgtcg tcgggccgat gcccgtgctt gccgtgcgcg
     3541 gtgcgacgcc agtgctccac gctcaggaag ccaccgtgga tcgctcccca gaccacgaac
     3601 gtccaggccg cgccgtgcca gagccctccc aacacgaacg tgatcatcaa gttgcgatag
     3661 gtccgccacc gtccgttgcg gttccctccg agcgggacgt acaggtagtc gcgcagccag
     3721 cgggacaacg tcatgtgcca gcggcgccag aagtcctgca gcgagaccgc ggtgtagggg
     3781 gcgtcgaagt tctgggggaa ccggaacccg agcaggagtg cgagcccgat cgccatgtcg
     3841 gtgtagccgc tgaagtcggc gtagatctgg atcgcgtacg cgtagaccga gaccaccacc
     3901 tcgagggagg agtgctgctt cgggttggcg aacacgatgt cgacgatctg ggttccgagg
     3961 aagttcgcga tcaccacctt cttgaacagg cccaggaaga tcaggaagaa cgcccggctc
     4021 gcgtcgaccc ggcgggcgtt gtgccgttcc cgcagctggg gcaggaactc ggcggcccgc
     4081 acgatcgggc ccgagatgac atgcgggaag aacgacaggt agacggcgaa ctcgaggaac
     4141 tctcggggct cgaacgttcc ccggtagatg tcgatgacgt agctgagggc ctggaacgtg
     4201 aagaacgaga tcccgaccgg cagcgtcacc gcgatgatgg tgttgccgac cgacaacccc
     4261 atcccgtcga ggacgtcgcg cgagtaccgc acg
//
LOCUS       DATCCH010000150         2115 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_588860, whole genome shotgun sequence.
ACCESSION   DATCCH010000150 DATCCH010000000
VERSION     DATCCH010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588860"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..1685
                     /locus_tag="VIJ44_03055"
     CDS             141..1685
                     /locus_tag="VIJ44_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEY5164910.1"
                     /translation="MPDDERPNILVIWGDDIGITNLSCYSDGLMGYRTPNIDRIADEG
                     MRFTDSYGEQSCTAGRSSFITGQSAFRTGLSKVGMPGADVGLQAEDATIAELLKPLGY
                     ATGQFGKNHLGDLNKYLPTAHGFDEFFGNLYHLNAEEEPESANWPPPEDFPGFNERFT
                     PRGVIHSWATDEDDAAEDPRYGRVGRQRIEDTGKLTRKRMETIDDEVLEHTLDFLDRK
                     ATEDAPFFCWFNTTHMHFRTHPKPESLGQAGRWQSPYHDTMIDHDKVVGAVLDKLDAL
                     GLSENTIVIYSTDNGPHMNTWPDGGMTPFRSEKNTNWEGAFRVPEMIRWPGKIPAGVV
                     SNEIVQHHDWLPTFLAAAGEPDIVEKLKAGHTIGETTYKVHIDGYNLLPFLTGEAEKS
                     PRPGLVYFSDDGDVLAIRFDNWKVVFMEQRVQGTLQIWAEPFVSLRVPKLFNLRTDPF
                     ERADITSNTYYDWLVGSDYIILAASTLVAQFLASFEEFPPRQKAASFTIDQVVAKLET
                     VLTSGR"
     gene            1682..>2115
                     /locus_tag="VIJ44_03060"
     CDS             1682..>2115
                     /locus_tag="VIJ44_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014399231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEY5164911.1"
                     /translation="MNRQAPASTMTWVPAATFRMGSDAHYPEEGPVHPVAVSGFWIDR
                     YAVTNRDFAAFVQDTGYVTVAERPLDPADFPGAPPENLVPGSLVFTPTPGQVDLRHFS
                     QWWTWTPAACWRRPEGPDSSLEDRPDHPVVQVAFEDAEQYAT"
BASE COUNT          400 a          734 c          650 g          331 t
ORIGIN      
        1 ccaccgctac gggcacatca tccgtcccag ccacctgcgc gactgacccg ccggccagca
       61 gtcaccccga caaggtgatg ttcgatcacc cggacaggcg ctggaatcgc accatatgac
      121 cattgcaaag gggaggccgc atgcccgacg atgagcgtcc gaacattctc gtcatctggg
      181 gcgatgacat cgggatcacg aacctgagct gttacagcga cggactgatg ggctaccgga
      241 caccgaacat cgatcggatc gccgacgagg ggatgcgatt caccgactcc tacggcgagc
      301 agagctgcac cgcgggccgt tcctcgttca tcaccggcca gagcgcgttc cggaccggcc
      361 tgagcaaagt cgggatgccg ggggccgacg tggggttgca ggccgaggac gcgacgatcg
      421 ccgagctgct caagccactc ggctacgcca ccgggcagtt cggcaagaac cacctgggcg
      481 atctgaacaa gtatctgccg accgcccacg gtttcgatga gttcttcggc aacctgtacc
      541 acctcaacgc cgaggaggag cccgagtcgg cgaactggcc gccgccggag gacttcccgg
      601 gcttcaacga gcgcttcacc ccgcggggcg tcattcattc gtgggcgacc gacgaggacg
      661 atgccgccga ggacccgcgc tacggacgcg tcgggcgtca gcggatcgag gacaccggca
      721 agctcacgcg caagcggatg gagacgatcg acgacgaggt gctggagcac acgctggact
      781 tcctcgatcg caaggccacc gaggacgcgc cgttcttctg ctggttcaac accacccaca
      841 tgcacttccg cacgcatccc aagccggaga gtctgggcca ggccggtcgc tggcagtcgc
      901 cgtatcacga cacgatgatc gatcacgaca aggtcgtcgg cgcggtgctc gacaagctcg
      961 acgcgctcgg tctgtccgag aacacgatcg tcatctacag caccgacaac gggccgcaca
     1021 tgaacacctg gcccgacggc ggcatgacgc cgtttcgcag cgagaagaac accaactggg
     1081 agggcgcgtt ccgcgttccc gagatgatcc gctggcccgg gaagatcccg gcgggagtgg
     1141 tttccaacga gatcgtccag catcacgact ggctgcccac attcctcgcg gccgccggcg
     1201 agcctgacat cgtcgagaag ctcaaggccg ggcacaccat cggcgagacg acctacaagg
     1261 tccacatcga tggctacaac ctgctgccct tcctcaccgg cgaggccgag aaatccccgc
     1321 gtcccggcct ggtctacttc tccgacgacg gcgacgtgct ggcgatccgg tttgacaact
     1381 ggaaggtcgt gttcatggag cagcgcgtcc aggggacgtt gcagatctgg gccgaaccgt
     1441 tcgtatcgct gcgggtgccc aagctcttca acctgcggac cgacccgttc gagcgcgcgg
     1501 acatcacctc gaacacctac tacgactggc tggtcggaag cgactacatc atcctcgccg
     1561 cctcgacgct cgtcgcccag ttccttgcct cattcgagga gttcccgcca cgacagaagg
     1621 cggcgagctt caccatcgac caggtcgtcg ccaagctgga gacggtgctg acatcaggac
     1681 gatgaaccgc caggcgccgg catccaccat gacgtgggtg ccggcggcga ccttccggat
     1741 gggctcggac gctcactacc ccgaggaggg accggtccac ccggtcgccg tcagcggatt
     1801 ctggatagac cgctacgcgg tgaccaaccg cgacttcgcc gcgttcgtac aggacaccgg
     1861 ctacgtcacc gtcgccgagc gtccgctcga tcccgccgat tttcccggcg ccccgccgga
     1921 gaatctcgtc ccgggatccc tcgtgttcac cccgacgccc ggacaagtcg acctgcgcca
     1981 tttctcgcag tggtggacct ggactcccgc ggcgtgttgg cgtcgtcccg agggcccgga
     2041 ctcctcactg gaggatcgcc ccgatcaccc cgtcgtccag gtcgccttcg aggacgccga
     2101 gcagtatgcg acttg
//
LOCUS       DATCCH010000151         2269 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_555225, whole genome shotgun sequence.
ACCESSION   DATCCH010000151 DATCCH010000000
VERSION     DATCCH010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555225"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="VIJ44_03065"
     CDS             <1..479
                     /locus_tag="VIJ44_03065"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY5164912.1"
                     /translation="LMDTSPGPPPGIDVATIDLGVEIARRDGMTVLKAVQDELDPLGT
                     PAYQRLLAERPGYREFCDRKWDALAPEMWVALVAELVRQPDQLAALTTVRCPTLVIVG
                     EQDTPFVGVSEEMAATIPGAQLTVVPDAGHSPQFENPRAWFDAVDDFLRAHARAPA"
     gene            476..2113
                     /locus_tag="VIJ44_03070"
     CDS             476..2113
                     /locus_tag="VIJ44_03070"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase"
                     /protein_id="HEY5164913.1"
                     /translation="MTEPDAGYLAAATLRVHDVDTVFTLNGGHLWPLYYGCTHAGVRL
                     VDTRHEQTAAFAAEGWAKVTRRPGVAALTAGPGVTNGVSAITTAFLNGSPVTIIGGRA
                     PQARWGSGSLQELDHVPILASVTKQADTAAAPEQVPGLLDGALRAARTPHRGPTFLDV
                     PLDCWGPSAARVPDALEPARLTGAVPDPEAIARVARLAGRAHRPVLMAGADVYWAGAE
                     AEVRAFADATRVPVFVNDMARGVIAADDPLAFSRARGRAFAEADLVIVAGTPLDFRLG
                     FGAFGDAAVVHLRDAEPLTAASVPRAAETTGDLRRTFASLAESAAHHGDATARAARED
                     WLAHLRDDEQARRAADEARLESDATPIDPVRIYGELRPRLERDAIVVGDGGDFVSYAG
                     RYIDTFTPGCFVGPGPYGCLGAGMGYALGAALAHPDRQVVALFGDGAIGFALGDLDTL
                     ARHDVNVTAVVGNNGIWGLEKHPMQLLYGEDLVADLAPETRYDLVAAALGVHGELVRD
                     PADLGGALDRALTHPGPALVNVLTDPADAYPRRSNLA"
BASE COUNT          307 a          909 c          742 g          311 t
ORIGIN      
        1 tgctgatgga caccagcccg gggccaccgc ccgggatcga cgtggcgacc atcgacctcg
       61 gggtcgagat cgcccgccgc gacgggatga cggtcctgaa agccgtccaa gacgaactcg
      121 acccgctcgg cacacccgcg taccagcggc tcctcgccga acgccccggg taccgcgagt
      181 tctgtgaccg caaatgggac gccttagcgc ccgagatgtg ggtcgcgctc gtggccgagc
      241 tggtgcgaca accggaccag ctcgctgcgc tcaccacggt gcgctgcccg acgctcgtca
      301 tcgtgggcga acaagacacc ccgttcgtcg gcgtgtccga ggagatggcc gccacgatcc
      361 ccggcgcgca gctcaccgtc gtccccgacg ccgggcactc accccagttc gagaaccctc
      421 gggcctggtt cgacgcggtc gatgacttcc tccgcgctca cgcccgggcg ccggcgtgac
      481 cgagcccgac gccgggtatc tcgccgccgc gaccctgcgg gtccacgacg tcgacaccgt
      541 cttcaccctg aacgggggcc atctctggcc cctctactac ggctgcaccc acgccggtgt
      601 ccgactcgtc gacacccgcc acgagcagac cgcggcgttc gccgccgagg gatgggcgaa
      661 ggtgacccgc cggcccgggg tcgcggcgct gaccgccggg cccggcgtga cgaacggggt
      721 gagtgccatc accaccgcgt ttctcaacgg atcgcccgtc accatcatcg ggggtcgcgc
      781 cccccaagcc cggtggggct cgggctcact ccaagagctc gaccatgtcc ccatcctcgc
      841 gtcggtcacc aaacaggcgg acactgccgc cgcgcccgaa caggtccccg ggctgctcga
      901 cggtgcgctc cgcgctgctc gcaccccgca ccggggcccg accttcctcg acgtgcccct
      961 cgactgctgg ggcccgagcg cagcccgagt tcccgacgcg ctcgaacccg cccgcctcac
     1021 cggggccgtt cccgaccctg aggcgatcgc gcgagtggcc cgcctggccg ggcgagcgca
     1081 tcgtcccgtg ctcatggccg gcgccgacgt gtactgggcc ggagccgaag ccgaggtgcg
     1141 ggccttcgcc gacgcgacgc gtgtgcccgt cttcgtcaac gacatggccc gcggcgtcat
     1201 cgccgccgat gacccgctgg ccttctcacg cgctcggggc cgggccttcg ccgaggccga
     1261 cctggtcatc gtggccggca ccccgctgga cttccgcctc gggttcggcg ccttcggcga
     1321 cgccgcggtg gtgcacctcc gcgacgccga gcccctcacc gcggcgtcgg tgccacgggc
     1381 ggcggagacg accggcgacc tgcggcgcac cttcgccagc ctcgccgagt ccgccgccca
     1441 ccacggcgac gcgacggcgc gggccgcccg tgaggactgg ctggcgcacc tccgcgacga
     1501 cgagcaggcc cggcgggccg cggacgaagc ccgactcgaa tcggacgcga caccgatcga
     1561 cccggtgcgc atctacggcg agctccgccc ccgtctggag cgtgacgcca tcgtcgtcgg
     1621 agacggcggc gacttcgtct cctacgccgg ccgctacatc gacaccttca cccccggctg
     1681 cttcgtcggc cccggcccct acggatgcct cggggccggg atgggctacg ccctcggtgc
     1741 cgcgctcgcc caccccgacc gccaggtcgt ggccctcttc ggcgacgggg ccatcgggtt
     1801 cgcgctcggc gacctcgaca ccctcgcccg ccacgacgtc aacgtcaccg ccgtcgttgg
     1861 caacaacggg atctggggac tcgagaagca ccccatgcag ctcctgtacg gcgaggacct
     1921 cgtcgccgat ctcgctcccg aaacccgcta cgaccttgtc gccgcggcgc tcggcgtgca
     1981 cggcgagctg gtgcgcgacc ccgccgacct cggtggcgcc ctcgaccggg cgctgaccca
     2041 ccccgggccg gcactcgtga acgtgctcac cgatcccgcc gacgcctacc cgcggcgcag
     2101 caacctggcg tgatgatccg cgtcgcggac gatcaggggc aggccttcca ggtgccgttc
     2161 tcgtcgacga tcaagatgct gaacgaccgg ctgctcctgc cgttgtcgta gctcaccgtg
     2221 aattcgacct tcgcggtgct acccgagcgg tcgacgccga acgggttcg
//
LOCUS       DATCCH010000152         2389 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_265318, whole genome shotgun sequence.
ACCESSION   DATCCH010000152 DATCCH010000000
VERSION     DATCCH010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265318"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            785..1378
                     /locus_tag="VIJ44_03075"
     CDS             785..1378
                     /locus_tag="VIJ44_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY5164914.1"
                     /translation="MTNLYHPGARHLQDRFDSRRIADRLEQITVHDTFTDDDRAFIER
                     CPMFFLATADAEGRPDCSYKGGRPGFVRVLDEHTLAFPSYDGNGMFRSLGNTVVNPNV
                     GMLFIDFEHPNRIRVNGIASLHDDDTLLDAFDGAQLVVQVHAERIFPNCPRYIHRMQL
                     VEHSVYAPSPGHVPPTPEWKKMEVFRDYLPGGPTSAP"
     gene            complement(1380..>2389)
                     /locus_tag="VIJ44_03080"
     CDS             complement(1380..>2389)
                     /locus_tag="VIJ44_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007034404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HEY5164915.1"
                     /translation="TGAAKLQEVAVLSTADEPSGDDSGVEDDRLRLIFTCCHPALSVE
                     AQVALTLRTLAGLTTAEIARAFLVPEATLAQRLVRAKRKIRHAGIPYRVPPAHLLPDR
                     TGAVLAVLYLLFNEGYAATAGADLVRRRLCGEAIRLARTLTDLMPDEPEALGLLALML
                     LHDARRAGRVDDAGDLVPLEEQDRGRWDRAVISEGLGLLDAALRRRQPGPYQVQAAIA
                     ACHASAPDAPATDWVEIAALYGELVRMVPSPVVELNRAVAVAMADGPEAGLRVVEALD
                     ASGSLQAYHLLPATRADLLRRLGRSADAAAAYRDALEFATSDAERRYLSRRLAETSAR
                     S"
BASE COUNT          409 a          933 c          747 g          300 t
ORIGIN      
        1 gaccaccgag ccccctcgtc ccacggacac cgcggtcggt ggagggtcca ccgcggcgta
       61 gaacccggcc ccggcgagga tcacccccaa gaacgcaccg gcccggagca gccacggcac
      121 ggcacgcgat cggtaccccg ccacgacccg ccgaacagag caacaacaca gctccgagga
      181 ccaggacggc ctcgaagggc cccaacccca acgcgatcac ctgactcagg tagtggaccg
      241 acgcctcccg caaggcatcg gtcgggccca ggatcttccc ggtcaagatc aacgcgtcga
      301 gcagcagccc cgcgcccctc cgacaaccac ccacagccgc cgcatcgcgg tccgcctcgc
      361 ctgctccgac gctcgcaccg catgttgtaa tggctcgctc gcagcgccac acgcgtccgg
      421 tcggtcgggg gcgggaccgc ccgggacggg aggcgctcag tcatccggcg gacctggagg
      481 atcaacggga atctcaacca gggcccgatg gtgctcgaac gcggcctgga ggctcaccac
      541 cgaggccacc ggtccgaagg agcacccaca ccgacaggtg gcgtcgtaga cgaggtccgc
      601 ccggtcgaac cccagcgggc cgaggcgatg cgccgccgcg cccccgggtt ccgtcgggca
      661 cgcatcccga tcactcgtcg cctcggggcc ggtgtcacca tgttgttcca ccaccaccct
      721 ggaccgaccg ccctcgttca agtgacgggc caaacgtcga acctgacgcc gtaccgtggg
      781 caccatgacc aacctgtacc accccggtgc acggcacctc caggaccgct tcgatagccg
      841 gcgcatcgcc gaccgcctcg agcagatcac ggtgcacgac accttcaccg acgacgaccg
      901 ggccttcatc gagcgatgcc cgatgttctt cctggccacc gccgacgctg agggccggcc
      961 cgactgctcc tacaagggcg ggcggcccgg cttcgtccgc gtcctcgacg agcacaccct
     1021 ggcgttcccg agctatgacg gcaacggcat gttccgaagc ctcggcaaca ccgtcgtgaa
     1081 cccgaacgtc gggatgctct tcatcgactt cgagcatccg aaccgcatcc gcgtgaacgg
     1141 catcgcctcg cttcacgacg acgacaccct gctcgacgcc ttcgatggcg cccagctggt
     1201 ggtccaggtc cacgccgagc gcatcttccc caactgcccc cggtacatcc accgcatgca
     1261 gctcgtcgag cactcggtct acgcacccag ccctggccac gtcccgccca cccccgagtg
     1321 gaagaagatg gaggtcttcc gcgattacct cccgggcggt cccaccagcg cgccgtgatt
     1381 caggaacgag cgctggtctc cgccaagcgg cgcgacaggt aacgtcgctc ggcgtcgctc
     1441 gttgcgaact cgagcgcgtc gcgatacgcg gcggccgcgt cggcggaacg ccccagccgg
     1501 cggagcaggt cggctcgggt cgccggaagc aggtggtacg cctgcaggga cccggaggcg
     1561 tcgagcgcct ccacgacccg aagcccagct tcagggccat cggccatcgc gaccgccacc
     1621 gcccggttca gctccacgac cggggagggc accatccgga ccaactcccc gtacagcgcc
     1681 gcgatctcga cccaatccgt cgcgggcgcg tccggcgcgc tggcatgaca cgcggcgatc
     1741 gcggcctgca cctgatacgg gcccggctgg cgccgccgca acgcggcatc gagcagcccg
     1801 agtccctcgc tgatcacggc ccgatcccag cggccgcggt cctgttcctc gagcggcacg
     1861 aggtcaccgg cgtcgtcgac ccgcccggcc cggcgggcgt cgtgcaagag catgagggcc
     1921 agcagcccga gcgcctcggg ctcgtcgggc atcaggtcgg tcagcgtccg ggccaggcgg
     1981 atcgcctcac cgcacaggcg gcggcgcacc aggtcagcac cagcggtcgc ggcgtacccc
     2041 tcgttgaaca gcaggtacag cactgccaac accgcgccgg tccgatccgg cagcagatgc
     2101 gccggaggga cccggtacgg gatcccggca tggcggatct tgcgcttcgc ccgaaccagt
     2161 cgctgggcga gcgtcgcctc cgggacgaga aacgcccggg caatctccgc cgtcgtgagg
     2221 cccgccaacg tccgcaacgt caacgccacc tgagcttcga ccgacaacgc cgggtgacaa
     2281 caggtgaaga tcagccgcag ccgatcatcc tcgacgccgc tgtcgtcacc actcggctcg
     2341 tccgcggttg aaagcacggc gacctcctgc agcttggcgg ccccggtcg
//
LOCUS       DATCCH010000153         2043 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_99826, whole genome shotgun sequence.
ACCESSION   DATCCH010000153 DATCCH010000000
VERSION     DATCCH010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99826"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="VIJ44_03085"
     CDS             <1..401
                     /locus_tag="VIJ44_03085"
                     /inference="COORDINATES: protein motif:HMM:NF018257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfocyanin-like copper-binding protein"
                     /protein_id="HEY5164916.1"
                     /translation="SVLAVGVSLAVRSTGPAGDVVAKTTNFRISMPTTLRPGRHTFAY
                     TNEGSVPHELLVFRTGLAGNSLPMRSDGDVAEESPLLHKVADSGSATAPGGIEAVPTN
                     EALVPGHYVAVCNLPEHYHLGMWLNITVAR"
     gene            519..962
                     /locus_tag="VIJ44_03090"
     CDS             519..962
                     /locus_tag="VIJ44_03090"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY5164917.1"
                     /translation="MAQAWLDELSLEECLALLRAGTIGRIAFTSDDWPVVFPVNYRLV
                     EASERTWIALRTRPGNVIDRSPLNVAFQVDGLGPMLEEGWSVLVRGTLHDVDPDAADF
                     RERFDPHPWIIDERDAWLIVEPFGITGRRLHAAEPEWAFHPEAYL"
     gene            complement(1008..>2043)
                     /locus_tag="VIJ44_03095"
     CDS             complement(1008..>2043)
                     /locus_tag="VIJ44_03095"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY5164918.1"
                     /translation="GDFVMLHDPQTAGLAREARLAGGIVVWRCHIGRDTANRYSDEGW
                     GFIRPYIEEANAFVFSRAEFIPPWIDGARSRVIPPSIDPFSAKNQDLDQTTVRLILQY
                     TGLLAGAADPPTVGFSRRDGSPGRIDRRADILQTGPPPPPEAPLVVQVSRWDRMKDMP
                     GVLRGFVEHLDGTRDAHLCLVGPAVHGVEDDPEAGEVLDECIRAWQELPATARSRVHL
                     ACIPMHDPDEAAIITNALQRHATVVTQKSVAEGFGLTVTEAMWKRRPVVASRIGGISD
                     QITDHEDGLLIDDPSDLDAFGGAVRLLLDDPDLAARLGKAAHQRVENEYLGDRHLERY
                     AQLFATLPER"
BASE COUNT          321 a          639 c          693 g          390 t
ORIGIN      
        1 cctccgtcct cgcggtcggt gtgtccctgg ccgtgcgctc gaccggtccg gctggcgacg
       61 tcgtggcgaa gacgaccaat ttccggatct cgatgccgac gaccctgcga cctgggcgtc
      121 acacgtttgc gtacaccaac gaaggatccg tgccccacga actcctggtg ttccgcactg
      181 gtctggcagg taattcgctt cccatgagga gcgatggcga cgtcgccgaa gagtcgcccc
      241 tgcttcacaa ggtggcggac agtgggagcg caacggctcc tggtgggatt gaagccgtgc
      301 cgaccaacga ggcccttgtc cctggtcact atgtcgcggt gtgcaacttg cccgagcact
      361 atcaccttgg tatgtggctc aatatcaccg ttgctcggta gcagctgggt tacgacttca
      421 gttgagccgt atggcttcgc ctggaagcgc cggccagtgg cagtggcgac ttgccgagcg
      481 ggttagcgtg cgttgaatcg ttgacgagtt gaggtcgcat ggcacaagct tggcttgacg
      541 agctctcgct tgaggagtgc cttgcgttgt tgcgtgcggg cacgatcgga cgcatcgcgt
      601 tcacgagtga cgactggcca gtggtcttcc ccgtgaacta ccggctggtc gaggccagtg
      661 aacgaacctg gatcgcgctg cggactcggc ccgggaacgt gatcgatcgc tcgcctctca
      721 acgtcgcgtt tcaggtcgac gggctcggcc ccatgctcga ggaggggtgg tccgtgctcg
      781 tccgcgggac cctgcacgac gtggatccgg acgcggcgga ctttcgagag cgcttcgatc
      841 cgcatccgtg gatcatcgat gaacgcgacg cctggctcat cgtcgagccg ttcggtatca
      901 ccggtcggcg tctgcatgcc gccgagcccg agtgggcatt ccaccccgag gcgtacctct
      961 gaccgagccg ttcgttcgct cggtgctggg ccgagtggtc gctggtgtca ccgctcgggc
     1021 aaggttgcga agagctgggc gtaccgctcg aggtgtcgat ccccgaggta ctcgttctcg
     1081 acgcgttgat gtgccgcttt gccgagtcgg gccgcgaggt cgggatcgtc gaggaggagc
     1141 cgcaccgcgc cgccgaaggc gtcgaggtcg gaaggatcat cgatgagcag accgtcttcg
     1201 tggtcggtga tctggtcgga gatgcccccg atccggctgg ccacgaccgg gcgacgtttc
     1261 cacatggcct cggtgacggt gagaccgaat ccttcggcga cactcttctg agtgacgacg
     1321 gtggcgtgcc gctggagcgc gttggtgatg atggcggctt cgtcggggtc atgcatcggg
     1381 atgcaggcca gatgcacccg gcttcgggcg gtcgccggga gttcctgcca cgcccggatg
     1441 cattcgtcga gcacctcccc ggcttcgggg tcgtcttcga cgccgtggac cgcggggccg
     1501 acgagacaca gatgggcgtc ccgagtgccg tcgagatgct cgacgaagcc ccgcagcaca
     1561 cctggcatat ctttcatgcg atcccagcgc gagacctgga ccaccagcgg cgcctccggc
     1621 ggtggagggg gaccggtttg gaggatgtcc gctcttcggt cgatccgtcc cggtgacccg
     1681 tcccggcgcg agaagccgac ggtcggcggg tcggcggcgc cggcgagcaa ccccgtgtat
     1741 tgcaggatca agcgaaccgt ggtttggtcc aaatcctggt tcttggctga gaacgggtcg
     1801 atcgaaggcg gaatcaccct gcttcgtgcc ccatcgatcc acggcgggat gaactcggcg
     1861 cgactgaaga cgaaggcgtt agcctcctcg atgtagggac ggataaatcc ccatccctca
     1921 tccgaatagc ggttcgccgt gtctcgcccg atgtggcaac gccacaccac gatccctccg
     1981 gctagacgcg cctcgcgagc caaccccgcg gtctgcgggt cgtggagcat gacgaaatcg
     2041 ccc
//
LOCUS       DATCCH010000154         3119 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_306660, whole genome shotgun sequence.
ACCESSION   DATCCH010000154 DATCCH010000000
VERSION     DATCCH010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306660"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="VIJ44_03100"
     CDS             <1..784
                     /locus_tag="VIJ44_03100"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HEY5164919.1"
                     /translation="GGGVVGDTAGFAAAVYHRGVAVVQVPTTLLAMVDAAIGGKTAVN
                     LPEGKNLVGAFHQPVAVVADVVTLATLPAAEYRSGLGEVAKYALMPGGGAVATLLAEH
                     TAAVLTRDPTVLTDLVAGCVAIKAAVVAADPEERTGVRAVLNYGHTFAHALETTGQYA
                     LAHGEAVSVGLVFAAHLARNLERIDDQTVEHVQRVLTGLGLPIRVPDDRSEPELLAVM
                     RRDKKVRDDLTFVLPGPHGLELVHDPPAPALTGAFRAVGVGG"
     gene            790..1245
                     /locus_tag="VIJ44_03105"
     CDS             790..1245
                     /locus_tag="VIJ44_03105"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEY5164920.1"
                     /translation="MAIILVLSGPNLNLLGEREPHIYGTATLDDLVGDIQAVASAAGH
                     AVEHVQSNHEGELVDAVQAARGRCAAIIVNAGALSHYSYALADALATYDGVAVELHIS
                     NPSARDAWRHLSVIAPVVTGTIAGFGRTGYRLAAEATVAALAAATPDAR"
     gene            1242..2351
                     /locus_tag="VIJ44_03110"
     CDS             1242..2351
                     /locus_tag="VIJ44_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019927052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEY5164921.1"
                     /translation="MTDSWAPMDVASRPARLRTRLPDAGVDALLVTRLPNIRYLTGFT
                     GSAALLLVTPDALVFITDGRYQTQAHEQLDAVGVDARVEIGATSAEQHDLLAAAVGPH
                     GRLGLEAHGVTWAQHRTLAPLVAADLVATDGLVEDLRRVKDPGEVARIRAACTIADDA
                     LAELLPTLADAPREREFAVELEYAMRRRGASAMSFDPIVAAGPNAAKPHARPTDRPIG
                     PGELVVIDFGCVVDGYCSDMTRTVSVDDPGPEARRLWDLVAASQQAGRDAVHAGVACA
                     DVDRASRAVIEDAGFGDQFVHGTGHGVGLEIHEAPRVARTAPGTLDVGTVVTVEPGVY
                     LPGVGGVRIEDTVVVTTDGAAPLTEAPKSLVVARH"
     gene            2364..2924
                     /gene="efp"
                     /locus_tag="VIJ44_03115"
     CDS             2364..2924
                     /gene="efp"
                     /locus_tag="VIJ44_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEY5164922.1"
                     /translation="MSISTNDLKNGMALNLPEGLMTVVEFQHVKPGKGGAFVRTKLKN
                     FRTGAVIERTFRADEKVPLAVIDKREMQYLYRDGDAYVFMDLADYEQLQIDGTNLGSA
                     VHYLKEGDHAVLPVYNSQVVGVELPAAVELAVTNTEPGVQGDRVSGARKAATLETGLV
                     IQVPLFVEPGEMVKVDTRSGEYLARA"
     gene            2934..>3119
                     /locus_tag="VIJ44_03120"
     CDS             2934..>3119
                     /locus_tag="VIJ44_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N utilization substance protein B"
                     /protein_id="HEY5164923.1"
                     /translation="MATRREARERALALCYELETKGQPAESVLADLPAPPDDYTVILV
                     RGVGEHEPEIDDLLRTYS"
BASE COUNT          482 a         1255 c          952 g          430 t
ORIGIN      
        1 cggcggcggt gtggtgggcg acaccgccgg cttcgccgcc gcggtgtacc accggggggt
       61 cgcagtggtg caggtcccga ccacgctgct ggcgatggtc gacgccgcca tcggcggcaa
      121 gaccgcggtg aacctccccg aaggaaagaa cctcgtcggc gcgttccatc agcccgtcgc
      181 cgtcgtcgcc gatgtcgtca ccctcgccac cctccccgcg gcggagtacc ggtccggtct
      241 cggtgaagtc gcgaagtacg cgctgatgcc cggcggcggc gccgtcgcca cactcctcgc
      301 cgagcacacc gccgcggtcc tcacccgcga cccgacggtg ctcaccgacc tcgtcgccgg
      361 atgtgtcgcg atcaaggccg cggtcgtcgc cgccgacccc gaggagcgca ccggcgtccg
      421 cgcggtgctg aactacgggc acaccttcgc ccacgcactc gagacgaccg ggcagtacgc
      481 gctggcccac ggcgaagccg tctccgtcgg cctggtgttc gccgcccacc tggcccgcaa
      541 cctcgaacgg atcgacgacc agaccgtcga gcacgtgcag cgggtcctca ccggcctcgg
      601 gctgccgatc cgcgtccccg acgaccggag cgaacccgaa ctgctcgccg tcatgcgccg
      661 tgacaagaag gtccgggacg atctgacctt cgtgctgccc ggcccccacg ggctcgagct
      721 cgtgcacgac ccgccggccc cggccctcac cggcgcgttc cgtgccgtcg gcgtgggagg
      781 ctgactgcgg tggccatcat cctggtgctg tccgggccga acttgaacct gctcggcgaa
      841 cgagaacccc acatctacgg caccgcgacc ctcgacgacc tcgtcggcga catccaggct
      901 gtggcgtccg ccgcgggtca cgccgtcgaa cacgtccagt ccaaccacga aggcgagctc
      961 gtcgacgccg tccaagcggc ccggggccga tgtgccgcca tcatcgtgaa tgctggtgcg
     1021 ctcagccatt actcgtacgc gctcgccgac gcgctcgcga cctacgacgg cgtcgcggtc
     1081 gagctccaca tctcgaaccc ctccgcccgc gatgcgtggc gtcacctctc ggtgatcgcc
     1141 cccgtcgtca ccggcaccat cgccggcttc ggacgcaccg gctatcgcct cgcggctgaa
     1201 gcaaccgtcg ccgccctcgc cgccgcgacc ccggatgctc gatgaccgac tcctgggcgc
     1261 cgatggacgt ggcgtcgcgc cccgcccgac tgcggacccg cctccccgac gccggcgtcg
     1321 acgcgctgct cgtcacccgc ctccccaaca tccgctacct gactggcttc accggctccg
     1381 ccgcgctgct cctcgtcacc cccgacgctc tcgtgttcat caccgacggc cgctaccaga
     1441 cccaggctca cgaacagctc gacgctgtcg gcgtcgacgc ccgcgtcgag atcggtgcca
     1501 ccagcgccga gcagcacgac ctcctcgccg ccgcggtcgg cccccatggc cgcctcggtc
     1561 tcgaagccca cggtgtcacc tgggcccagc accggaccct ggcgcccctc gtcgccgcgg
     1621 acctcgtcgc caccgacggg ctggtcgagg acctacgccg cgtcaaggac cctggcgaag
     1681 tggcccgcat ccgggccgcg tgcaccatcg ccgacgacgc cctcgccgag ctcctcccga
     1741 ccctcgccga cgcgccccgc gaacgggagt tcgcggtcga gctcgagtac gccatgcgcc
     1801 gccgaggggc cagcgccatg agtttcgatc ccatcgtcgc cgcgggaccg aacgccgcca
     1861 aaccccacgc ccgaccgacc gaccgtccca ttggaccagg cgagctcgtg gtcatcgact
     1921 tcggctgcgt cgtcgacggc tactgctccg acatgacccg aaccgtcagc gtcgacgacc
     1981 ccggccccga ggcccgccgg ctctgggacc tcgtcgccgc gagccaacaa gccggccgcg
     2041 acgcggtgca cgccggcgtc gcctgcgccg acgtcgaccg cgccagccgg gctgtcattg
     2101 aagacgccgg cttcggcgac cagttcgtgc acggcaccgg ccacggcgtc ggcctcgaga
     2161 tccacgaggc cccccgagtc gcccgaaccg cgcctggtac cttggatgtc ggcaccgtcg
     2221 tcacggtcga acccggcgtc tacctccccg gcgtcggtgg cgtccgcatc gaggacaccg
     2281 tggtcgtcac caccgacggc gccgcgcccc tcaccgaagc ccccaagtcg ctcgtcgtcg
     2341 cgcggcactg acccaggacc cccgtgagca tctccaccaa cgacctcaag aacgggatgg
     2401 ccctcaatct ccccgaaggg ctcatgaccg tcgtcgagtt ccagcacgtc aagccgggca
     2461 aaggcggcgc cttcgtccga accaagctca agaacttccg caccggcgcg gtcatcgaac
     2521 ggaccttccg cgccgacgag aaagtccccc tcgccgtcat cgacaaacgc gagatgcagt
     2581 acctctaccg ggacggcgac gcgtacgtct tcatggatct cgccgactac gagcagctcc
     2641 aaatcgacgg caccaacctc ggttccgccg tccattacct caaggagggc gaccacgctg
     2701 tcctccccgt gtacaactcc caagtcgtcg gtgtcgagct ccccgccgcg gtggagctgg
     2761 ccgtcaccaa caccgagccc ggtgtccaag gcgaccgggt ctcgggcgcc cgcaaggccg
     2821 ccaccctcga gaccggtctc gtcatacaag tgccgttgtt cgtcgaaccg ggtgagatgg
     2881 tcaaggtcga cacccgctct ggggagtacc tcgcgcgagc gtgaggggac gacatggcga
     2941 cacgacgcga ggcacgggaa cgggccttgg cgctgtgcta cgagctcgaa accaaaggcc
     3001 aacccgccga gtcggtcctc gccgaccttc cggccccacc ggacgactac accgtcatcc
     3061 tcgtccgagg cgtcggcgag cacgaacctg agatcgacga cctgctccgc acctactcc
//
LOCUS       DATCCH010000155         3789 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_473253, whole genome shotgun sequence.
ACCESSION   DATCCH010000155 DATCCH010000000
VERSION     DATCCH010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473253"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VIJ44_03125"
     CDS             complement(<1..378)
                     /locus_tag="VIJ44_03125"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY5164924.1"
                     /translation="MADEAHEHTTVGAPPDRCYAVAVDFERYPEWARDVKEVTVLERD
                     AEGRSARVEYRVAGLGQSIHYVLEYDYGDAPDSFSWQLVESDKLRQLDGRYGFEPEGT
                     GTRVAYDLTIDVAIPLPGLIRRQA"
     gene            complement(422..844)
                     /locus_tag="VIJ44_03130"
     CDS             complement(422..844)
                     /locus_tag="VIJ44_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164925.1"
                     /translation="MSEGYDGRRIVVTSWVGVALFGLTAVPAAAGVSGFDVAALVVDV
                     ALFLVGIAVFFWAFAAALVRNAHGDDIVVSSLFLTQGPVPRPVRRNLFAALAISVVIA
                     GATGTTDAFGILVPMYPLGLIGLWGARHGVFPPRRDRR"
     gene            complement(870..2003)
                     /locus_tag="VIJ44_03135"
     CDS             complement(870..2003)
                     /locus_tag="VIJ44_03135"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY5164926.1"
                     /translation="MTSSLLVTNDFPPKIGGIQSYLYELWRRLPAPDTTVLTTPHVNA
                     ADWDHAQPFRVERTRQRFLLPTSVLARHVDALAREIDAGIVFVDPMLPLGHLVPRLAD
                     RPTIVIAHGAEITVYGRLPGTAPLARRVLRHATGLVAAGQYPSEQAIRVAGHSVPTLV
                     IPPGVDSERFTPRDDAARAATRMRFGLDVDAPLVLGVSRLVPRKGFDVLLDAVAGLDG
                     VQVAIAGTGRDAARLARRAARLGGRARLLGRVLDAELPDLYASADVFAMLCRDRWAGL
                     EAEGFGIVFLEAAACGVPAVAGRSGGADEAVIDGVTGVVVEPRDVAAVRAALDRLLSD
                     EVLRARLGAAARRRAVGEYSYDRLAARLARLAAGDLAALEAIG"
     gene            complement(2021..2686)
                     /locus_tag="VIJ44_03140"
     CDS             complement(2021..2686)
                     /locus_tag="VIJ44_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018158541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY5164927.1"
                     /translation="MSLTSVQPADAGGVAVLTLERPEKRNALSIALRDEMSDALDHLA
                     AADTTRAVVITGTGEVFSAGFDLDEFAANELAARLWASSDRWHRTVLTFPLPTVAAVN
                     GAALGGGFDLAVMCDVRICADIARFGHPEHRFSQVVYGPLHDLVGGARARDLALTGRR
                     VDATEALALGLVTRVVPAESLLGEAVELAREIAQAPREVLLAMKAKINRRAGVEPGAT
                     LDL"
     gene            complement(2683..>3789)
                     /locus_tag="VIJ44_03145"
     CDS             complement(2683..>3789)
                     /locus_tag="VIJ44_03145"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5164928.1"
                     /translation="AEPHQPYDRPPLSKQVLAGEWEPDQTALRSRPYDDLDLDWRLGC
                     RAAALDLAERAVILGHGERLAFTGAVLATGAIPRTLPDTPPLAGIHVLRTLDDCLALR
                     AELEHHPQVVVVGAGFIGAEVAATCRGRGLDVTVLEALPAPMVRGLGPLLGDALGALH
                     RDHGVDLRLRTSVAGFEGHERVERVLLGDGTTIPADVVVVGIGVRPATDWLEGSGVTI
                     EDGVVCDDTCLAAPGVVAAGDLARWPNPLFDGESMRLEHWTNAAEQGVYAGRRLLSSD
                     AVAPFAPVPFVWSDQYDVKIQVAGRVRGDDRIEIVDGAIDELRFVAAVGREGRLVGAV
                     AFNRPRVLMQYRRLIAERCSFDDALAASERQGAR"
BASE COUNT          585 a         1275 c         1444 g          485 t
ORIGIN      
        1 cgcctgtcgt cggatcaggc cgggcagggg gatcgcgacg tcgatcgtga gatcgtaggc
       61 gacacgggtg ccggtcccct cgggctcgaa gccgtatcgg ccgtcgagct ggcgcagctt
      121 gtcgctctcg acgagctgcc acgagaacga gtcgggcgcg tcgccgtagt cgtactcgag
      181 cacgtagtgg atgctctggc ccaggccggc gacgcggtat tccacccgtg cgctgcgccc
      241 ttcggcgtct cgttcgagca cggtgacttc cttcacgtcc cgggcccatt cgggatagcg
      301 ctcgaagtcg acggcgaccg cgtagcagcg gtcgggagga gcgccgaccg tggtgtgctc
      361 gtgggcctcg tccgccatac cggaatcgaa gcaagccgcg cctggttgcg caacctggcg
      421 ctcagcgacg gtcccggcga ggtgggaaca ccccgtggcg ggcgccccag aggccaatga
      481 gtcccaacgg gtacatgggg acgaggatgc cgaacgcgtc ggtcgtgccg gtcgcgccgg
      541 cgatcaccac cgagattgcg agcgcggcga agaggttgcg gcgcaccggt cgtgggaccg
      601 ggccctgggt gaggaacagc gaggagacga cgatgtcgtc gccatgggcg ttgcggacca
      661 gggccgccgc gaacgcccag aagaacacgg cgatcccgac gaggaagagc gcgacatcaa
      721 caacgagggc ggccacgtcg aaacccgaga cccccgcggc ggccggaacc gcggtgagcc
      781 cgaacagcgc gacaccgacc cacgaggtga cgacgatccg cctgccgtcg tacccctccg
      841 acacgcgctg agcgtaccgg ggtagtgggt cagccgatcg cctcgagtgc cgcgaggtct
      901 ccggcggcga gccgggccag acgcgccgcg aggcggtcat aggagtactc ccccaccgcc
      961 cggcggcgtg ccgcggcgcc gagtcgggcg cgcagcacct cgtcgctcag cagccggtcg
     1021 agggcggcgc gcaccgcggc gacgtcgcgg ggctcgacga cgacaccggt cacgccgtcg
     1081 atgaccgcct cgtccgcgcc gccgctgcgg ccagccaccg cgggtacgcc acacgccgcg
     1141 gcttcgagga agacgatgcc gaagccttca gcctcgaggc cggcccagcg gtcgcggcac
     1201 agcatggcga acacgtccgc cgacgcgtac aggtcgggca actcggcgtc gaggacgcgc
     1261 ccgaggagcc gagcccgtcc tccgagccgg gccgcgcggc gggccagtcg ggccgcgtct
     1321 cggccggtgc ccgcgatcgc cacctggacg ccgtcgagac ccgcgacggc gtcgaggagg
     1381 acgtcgaatc ctttccgggg gacgagtcgg ctcacgccca gcacgagggg cgcgtcgacg
     1441 tctaacccga accgcatgcg ggtggcggcg cgggcggcat cgtcacgggg tgtgaaccgt
     1501 tcggagtcga cgcccggcgg gatgacgagc gtcggcaccg agtggcccgc gacgcgaatc
     1561 gcctgctcgg acgggtactg gccggcggcc acgagcccgg tggcgtgacg caacacgcgt
     1621 cgggcgagtg gggccgtgcc ggggaggcgc ccgtacacgg tgatctccgc gccgtgagcg
     1681 atgacgatgg tggggcgatc ggccaggcgc ggcacgagat ggccgagcgg cagcatcggg
     1741 tccacgaaga cgatgcccgc gtcgatctca cgggcgaggg cgtcgacgtg gcgagccagc
     1801 accgaggtgg ggagtaagaa gcgctgtcgg gttcgctcga cgcggaaagg ctgcgcgtgg
     1861 tcccagtcgg ccgcgttgac gtgcggggtg gtgagcacgg tggtgtcggg cgcgggcagt
     1921 cgacgccaca gctcgtagag ataggactgg atcccaccga tcttgggtgg gaagtcgttc
     1981 gtgacgagca gcgacgacgt caccggagcg tgacccggcg tcagagatcg agggtggcgc
     2041 cgggttcgac gccagcccgc cggttgatct tggccttcat cgccagcagc acctcgcgag
     2101 gggcttgcgc gatctcccgg gcgagctcga cggcttcgcc gaggagcgac tcggccggga
     2161 ccactcgagt gacgagcccg agagcaaggg cctcggtggc gtcgacgcgc cggccggtca
     2221 gggccaagtc gcgcgcccgg gcaccgccga ccaggtcatg gagtggtccg tacacgacct
     2281 ggctgaaccg gtgctccgga tgcccgaagc gggcgatgtc ggcgcagatc cgcacgtcgc
     2341 acatcaccgc caggtcgaag ccgcctccga gcgcggcccc attgacggca gccacggtcg
     2401 gcagggggaa ggtgaggacg gtgcgatgcc accggtcgct ggaggcccag agccgggcgg
     2461 cgagctcgtt ggccgcgaac tcgtcgaggt cgaagccggc gctgaatacc tcgccggtcc
     2521 cggtgatcac caccgcccgg gtggtgtcgg cggccgcgag gtggtcgagt gcgtcggaca
     2581 tctcgtcgcg cagggcgatg gagagtgcgt tgcgtttctc gggtcgctcc agggtgagca
     2641 ccgcgacacc gccggcgtcg gctggttgga cggaggtgag gctcatcgcg ctccttggcg
     2701 ctctgaggcg gcgagggcgt cgtcgaacga gcaccgctcc gcgatcaggc gccgatactg
     2761 catgaggact cggggccggt tgaacgcaac ggcgccgacg aggcgccctt cgcgcccgac
     2821 cgcagcgacg aatcggagct cgtcgatcgc gccgtcgacg atctcgatgc ggtcgtcacc
     2881 gcgaacccgg cccgcgacct ggatcttgac gtcgtactgg tccgaccaga cgaacgggac
     2941 gggggcgaag ggggcgacgg cgtcggagct gaggagtcgc cgacccgcgt agacgccctg
     3001 ctcggcggcg ttggtccagt gctcgaggcg catcgattca ccgtcgaaga gcgggttcgg
     3061 ccagcgggcg agatcaccag ctgcgacgac gccaggggcg gcgagacagg tgtcgtcgca
     3121 caccacgccg tcttcgatgg tgactcccga gccttcgagc cagtcggtgg ccgggcggac
     3181 gccgatcccg acgacgacga cgtcggccgg gatcgtggtg ccatcgccga gcaagacacg
     3241 ttcgacgcgc tcgtgtccct cgaacccggc gacgctggtt cgcagccgga ggtccacacc
     3301 gtggtcgcgg tgcagcgcac cgagcgcgtc gccgagcagc ggtccgaggc cccggaccat
     3361 cggtgccggg agcgcctcca ggacggtcac gtcgagtcct cggccccggc aggtcgcggc
     3421 gacctcggcg ccgatgaacc ccgcgccgac caccacgacc tggggatggt gctcgagttc
     3481 ggcgcgcagc gcgagacagt cgtcgagggt gcgcagcaca tggatgccgg cgaggggcgg
     3541 cgtgtcggga agggtgcgcg ggatcgctcc ggttgccagc accgcgcccg tgaacgcgag
     3601 tcgttcaccg tgaccgagga tcacggctcg ctcggcaagg tcgagcgccg cggcgcgaca
     3661 gcccagccgc cagtcgagat ccaggtcgtc gtagggccgt gagcgcagcg cggtctggtc
     3721 cggctcccac tcgcccgcga gcacctgctt ggagagcggc ggacggtcat acggctggtg
     3781 cggctcggc
//
LOCUS       DATCCH010000156         5022 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_306711, whole genome shotgun sequence.
ACCESSION   DATCCH010000156 DATCCH010000000
VERSION     DATCCH010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5022
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306711"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1009)
                     /locus_tag="VIJ44_03150"
     CDS             complement(<1..1009)
                     /locus_tag="VIJ44_03150"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY5164929.1"
                     /translation="MSLPDNDLDGTDFPPELDEQDEGALDVAPPVVVVVVTRNPGPFL
                     ESALEGLGSQDYPTLSVLVVDAGSTVDPGPRVAAVLPDAFVHRGPGSRPGFGGAANEA
                     LELVDGAAFFLVCHDDAVLAPDAVRLVVEEAYRSNAAIVGPKLVSSDDPEALLEVGRT
                     IDRLGGSHTGIEPGELDQEQHDAVRDVFYVSSAALLVRADLFAELGGFDPETFPGSED
                     LDLCWRARLAGARVVVAPDARAAHVEAASARRPGDAPTARDVARRRVRVLLTCYSFSS
                     LLRVIPIGIALAFFEALVFAPTPRRRQATAVIGAWRWNLVHLARLRAARRRAQALRTI
                     HD"
     gene            1137..1568
                     /locus_tag="VIJ44_03155"
     CDS             1137..1568
                     /locus_tag="VIJ44_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164930.1"
                     /translation="MTSDTIIRDARQRAEDAAYTVVGIGVLGFQQAQVRRRAAQGRLT
                     ETAKTARDQATNVVSDARAAAESVRTEVKTRVEPLVAQFGERVEPLVADVRARVEPVL
                     EQVSVTVRRVVDEVNPTTHPSAKASPKGTTTTANPATTPTD"
     gene            1633..2571
                     /gene="cofD"
                     /locus_tag="VIJ44_03160"
     CDS             1633..2571
                     /gene="cofD"
                     /locus_tag="VIJ44_03160"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HEY5164931.1"
                     /translation="MLAALAGGVGAARFLCGLVDVVTPEEVTAVVNTGDDDEFHGLWV
                     CPDLDSVTYALAGANNPETGWGLAGETFDTMDALARYGTSTWFRLGDRDLATHLYRSA
                     RRRAGVSLTEVTAEIATAWGLGTRLLPMSDDAVATRITVRAPDGTPQELALQEWFVRD
                     RAEPPVLSVRFEGASRSRPAPGVIEALSAAETILICPSNPVISIGPILAVPGLRELLR
                     ARRDRVVGVSPIIGGRPVKGPADRLMGPLGIEVSCVGVAEAYRDVCATLVIDTADADA
                     APRIEALGVHAVVADTLMRDERIAAALARDTLAAVA"
     gene            2568..3275
                     /gene="cofE"
                     /locus_tag="VIJ44_03165"
     CDS             2568..3275
                     /gene="cofE"
                     /locus_tag="VIJ44_03165"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEY5164932.1"
                     /translation="MTLTIIPVAGIPEVRPGDEVAILVTDAARAQGTPLTDGDCLVVT
                     QKVMSKAEDRLVRLDPDDRDARRALIDSESVRILRRRGDLVISETRHGFVCANAGIDL
                     SNVDAGWAALLPVDPDRSARHVRDAVRAHAAVDLAVIVSDTFGRAWRLGLTDVAIGVA
                     GLAAVVDLRGETDALGRELQVTEVAIADEIAGAAELVMGKARAIPAAIVRGLEGSWRR
                     EGSARELIRAPGDDLFR"
     gene            3272..3880
                     /locus_tag="VIJ44_03170"
     CDS             3272..3880
                     /locus_tag="VIJ44_03170"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEY5164933.1"
                     /translation="MSDVPAFLEARRSIRAFRPEPVDPNVLDGLVTAACLAPAPHHSR
                     PWRWVVVDTTDAKAALARGMGERWRSDLARDGLDTARIDALVDASHRRIEAAPAVVLG
                     CLTDDGLDHYPDEARQRAEWGMALLALGAAVENLMLAATNRGLASCWVAAPIFCPHEA
                     RTSLRLDDGWLPHAMVLVGHADPTYEPRPRPPVELESLRARR"
     gene            complement(3881..4831)
                     /locus_tag="VIJ44_03175"
     CDS             complement(3881..4831)
                     /locus_tag="VIJ44_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020458606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY5164934.1"
                     /translation="MRVLVTGGAGFIGSTLVDRLLAEGSDVDVVDDLSSGLLANLADA
                     RAQRARKCTFQRLDVRSKGVIELMAHRKPEVVYHLAAQPSVTVSVNEPTLDAEVNILG
                     SLNVLQGALQAGTRKVVFAGSGGTLYGVPESTPTREGHPQHPISPYGVSKKAVGDYLH
                     YYREVHGLESTVLALANVYGPRQDPHGEAGVVAIFAGRLLTGERPTIFGDGQQTRDFV
                     FVDDVADAFVRATEKAGGLTVNIGTGSETSVQQLYDLMARTVGFEEPARYAPPRAGEL
                     RRSALDPGRAAIHLAWKPWTTLDEGVQRTVDWFGAQRVGR"
     gene            complement(4828..>5022)
                     /locus_tag="VIJ44_03180"
     CDS             complement(4828..>5022)
                     /locus_tag="VIJ44_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HEY5164935.1"
                     /translation="VVHDRARIGPGARVTCSIVGPGAVIGAQATIRDESIVGADTVVD
                     PGSELIDSRVPESNAVPAPS"
BASE COUNT          756 a         1831 c         1740 g          695 t
ORIGIN      
        1 cgtcgtgaat ggtccgcagc gcctgcgccc ggcgccgagc ggcgcgcaac cgggcaagat
       61 ggaccaggtt ccagcgccac gcgccgatga ccgcggtcgc ctggcgacgg cgcggtgtcg
      121 gcgcgaacac cagcgcctcg aagaacgcca gcgcaatacc gatcgggatg acgcgcaaca
      181 gcgacgagaa cgagtagcag gtgagcagga cgcggacgcg acggcgagcg acgtcgcggg
      241 cggtcggggc gtcaccgggg cgacgcgccg aggcggcctc gacgtgcgcg gcccgggcgt
      301 cgggggccac gacaacccgg gcgccggcga ggcgtgcccg ccagcagagg tcgaggtcct
      361 cggagccggg gaacgtctcg ggatcgaagc cgcccagctc ggcgaacagg tcggcgcgca
      421 cgagcagcgc ggcgctcgag acgtagaaga cgtcgcggac cgcgtcgtgc tgctcttggt
      481 ccagctcccc cggttcgatg ccagtgtgcg accctccgag ccggtcgatc gtccggccca
      541 cctcgagcag ggcctcgggg tcgtccgacg acacgagttt cggtcccacg atcgcggcgt
      601 tcgagcggta ggcctcctcg accaccaggc ggaccgcgtc gggcgccaac acggcgtcgt
      661 cgtggcagac gaggaagaac gcggccccgt cgaccagctc gagggcctcg ttggccgcgc
      721 caccgaatcc cgggcggctt ccgggccccc gatggacgaa ggcgtcgggc agcaccgcgg
      781 cgacccgcgg cccgggatcg acggtggatc ctgcgtcgac gaccagcacc gacagcgtcg
      841 ggtagtcctg ggacccgagc ccttcgagcg ccgattcgag gaacgggccc ggattacggg
      901 tcacgacgac gaccacgacg ggcggcgcga catcgagggc gccctcgtcc tgctcgtcga
      961 gctcgggcgg gaagtcggtt ccgtcgaggt cgttgtcggg caggctcatc gggtggcctg
     1021 aggataggcg ggaccttgcc cgtactcatg accggtcaca agtgcttgca atagttagca
     1081 gtctcgcgta ttgtggttag caacgatccc ccaccgatcc ttcgaggaga acaatcgtga
     1141 ccagtgacac catcatcaga gacgcccgcc agcgtgccga ggacgccgcc tacaccgtgg
     1201 tcggcatcgg cgtgctcggg ttccagcagg cccaggtccg ccgccgggcg gctcagggcc
     1261 gcctgaccga gacggccaag accgcccggg accaggccac caacgtcgtc agcgacgccc
     1321 gagccgcggc cgaatcggtc cgcaccgagg tcaagacccg cgtcgagccc ctcgtggccc
     1381 agttcggcga gcgagtcgag cccctcgtgg ccgacgtgcg cgcccgcgtg gagccagtcc
     1441 tcgagcaggt cagcgtcacc gtccgtcggg tcgtcgacga ggtcaacccg acgacgcatc
     1501 cgtcggcgaa ggccagcccg aagggcacga ccacgacagc gaacccggcg accaccccga
     1561 ccgactgacc ccaaccgcca ccggcacgaa ccccccggtc ccaccggggg gttcgtcgcg
     1621 taacgtctcg gcgtgctcgc cgcgctggcc ggaggtgtcg gggccgcccg attcttgtgc
     1681 ggcctggtcg acgtcgtcac gcccgaagag gtgaccgcgg tcgtcaacac cggcgacgac
     1741 gacgagttcc acggcctgtg ggtgtgtccc gacctcgaca gcgtcaccta cgccctcgcg
     1801 ggggcgaaca accccgagac cggctggggt ctcgccggtg agaccttcga cacgatggac
     1861 gcgttggcgc gatacgggac ctccacctgg tttcggctgg gtgaccgtga cctggccacc
     1921 cacctgtacc gaagcgcccg tcgacgcgcc ggcgtgtccc tgaccgaggt gacggccgag
     1981 atcgcgaccg cgtggggctt gggaacccgg ctcctgccga tgagcgacga cgccgtcgcg
     2041 acccggatca cggttcgggc ccccgacgga acgccacagg agctggccct gcaggagtgg
     2101 ttcgtccgcg accgagccga acctccggtc ttgagcgtcc gcttcgaggg ggccagccga
     2161 tcccgccccg ctcccggcgt catcgaggcg ctcagcgccg ccgagacgat cctgatctgc
     2221 ccgagcaatc cggtgatctc gatcgggccg atcctcgccg tcccgggcct ccgtgagctg
     2281 ctccgcgctc gccgagatcg cgtcgtcggc gtcagcccga tcattggcgg ccgaccggtc
     2341 aaagggccag ctgatcgact catgggcccg ctcggcatcg aagtgtcctg cgtcggcgtc
     2401 gccgaggcct accgcgacgt ctgcgccacc ttggtgatcg acaccgccga cgcggacgcc
     2461 gcaccccgca tcgaagcgct cggcgtgcac gccgtcgtcg ctgacacctt gatgcgcgac
     2521 gagcgcatcg ccgccgcgct cgcacgcgac acactcgctg ccgtggcgtg acgctcacca
     2581 tcatcccggt cgcagggatc cccgaggttc gccccggtga cgaggtcgcg atcctcgtca
     2641 ccgacgcggc ccgagctcaa ggcacgcccc ttaccgacgg cgactgtctg gtcgtgaccc
     2701 agaaggtgat gtcgaaggcc gaagatcgcc tcgtccgcct cgaccccgac gaccgcgacg
     2761 cgcgccgggc gctcatcgat tcagaatcgg ttcggatctt gcgacgccga ggcgacctgg
     2821 tgatctccga gactcgccac gggttcgtat gcgccaacgc ggggatcgac ctgtcgaacg
     2881 tcgacgccgg gtgggccgcc ctgctacccg tcgatcccga ccggtccgcc cgtcatgtgc
     2941 gcgacgcggt gcgggcccac gccgccgtcg acctcgcggt gatcgtctcc gacaccttcg
     3001 ggcgcgcctg gcgtctagga ctgaccgacg tcgccatcgg agtcgccggc ctcgccgcgg
     3061 tcgtcgacct tcgcggcgag accgacgcgc tcggccgaga gctccaggtc accgaggtgg
     3121 ccatcgcgga cgagatcgcg ggtgccgccg agctggtcat gggcaaggcc cgggcgatcc
     3181 cagccgccat cgtgcgcggc ctcgaagggt cctggcggcg cgaagggtca gcccgcgagc
     3241 tgatccgcgc gccgggtgac gacctgttcc gatgagcgac gtccccgcat ttctcgaggc
     3301 gcggcgctcg atccgcgcct ttcgccctga gcccgtcgat ccgaacgtgc tcgatgggct
     3361 cgtcacggcg gcgtgcctgg cccccgcgcc ccaccattcc cgaccgtggc gctgggtcgt
     3421 ggtcgacacg accgacgcga aggccgcact ggcccgcggg atgggcgaac gctggcggtc
     3481 cgacctggcc cgcgacgggc tcgacacggc gcgcatcgac gccctcgtcg acgcatctca
     3541 ccggcgcatc gaggccgcac cggcggtggt gctcggatgc ctcaccgacg acggcttgga
     3601 ccactatccc gacgaggcgc gccaacgcgc cgagtggggc atggcgctcc tcgcgctcgg
     3661 cgccgccgtc gagaacctga tgctcgccgc caccaaccgg ggcttggcgt cgtgctgggt
     3721 ggcagccccg atcttctgtc cccacgaggc ccgcacgtca ctccgactcg acgacggatg
     3781 gctcccccac gccatggtcc tcgtgggcca cgccgacccg acctacgagc cgcgcccccg
     3841 accgccggtc gagctggaat cgctgcgagc ccgccggtaa tcagcgtccg acgcgttgag
     3901 cgccgaacca gtcgaccgtg cgttgaacgc cctcgtcgag ggtggtccac ggcttccagg
     3961 ccaagtggat cgcggcccgg ccagggtcca acgccgaacg tcgaagctcg ccggctcgag
     4021 gcggcgcgta gcgcgccggt tcctcgaacc cgacggtgcg ggccatgagg tcgtagagct
     4081 gctgcacgct ggtctctgat ccggttccga tgttgacggt caggccgccc gccttctcgg
     4141 tggcgcggac gaaggcgtcc gccacgtcgt cgacgaacac gaagtctcgg gtctgctgcc
     4201 cgtcgccgaa gatggtcggc cgctcgccgg tgagcagccg accggcgaag atcgcgacga
     4261 caccggcttc accgtggggg tcctggcgag gcccgtacac attggcgagg gccagcaccg
     4321 tcgactccag gccgtgtacc tcgcggtagt agtggagata gtcgccgaca gccttcttgg
     4381 acacgccgta cggcgagatc ggatgctgcg gatggccctc acgggtcggg gtggactcgg
     4441 ggacgccgta cagcgtgccg ccggacccgg cgaacacgac cttgcgggtg ccggcctgca
     4501 gtgcgccctg cagcacgttc agcgacccga gaatgttcac ctcggcgtcc aaggtcggct
     4561 cgttgaccga gacggtcacg ctcggctgcg cggccaagtg gtacacgacc tcgggcttgc
     4621 ggtgcgccat cagctcgatg acgcccttcg accgcacgtc gagacgctgg aaggtgcact
     4681 tgcgggctcg ctgtgcgcgc gcgtcggcga gatttgcgag caacccgctt gagaggtcat
     4741 cgacgacgtc gacgtcggat ccctccgcga gcagccggtc gacgagcgtc gacccgatga
     4801 agccggcgcc cccggtgacc agaaccctca tgacggcgcc gggaccgcgt tcgattccgg
     4861 cacccggctg tcgatcagct cggagcccgg gtccacgacg gtgtcggcac cgacgatcga
     4921 ttcgtcgcgg atcgtcgcct gcgcgccgat gaccgcgccc ggcccgacga tcgagcaggt
     4981 gacccgcgcc ccgggtccga tgcgcgcccg gtcgtgaacg ac
//
LOCUS       DATCCH010000157         4037 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_589064, whole genome shotgun sequence.
ACCESSION   DATCCH010000157 DATCCH010000000
VERSION     DATCCH010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4037
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589064"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="VIJ44_03185"
     CDS             complement(<1..729)
                     /locus_tag="VIJ44_03185"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY5164936.1"
                     /translation="MRVLVTGMGGELGTRVAQLLETRPEVDEIAGFDFMPPRRRLRRA
                     TFKRIDPRDRDRLIGFVTDFAPHAVAHFGVYEPGSRMGSREAAASTEACTVNALGAAQ
                     RAGHLGRIAVRSGLEVYGRGRGHPLVPDEEAPLAPTTPYGRTCLEVESLALGIGRRHD
                     VPVGALRYAVVAGSHIPSPLGRVLRLPVVPVPALADPPFSLLHQDDAARAMVEMLVQG
                     FEGPLNVVGPGATSPWQAVRLGGRI"
     gene            complement(729..2243)
                     /gene="lysS"
                     /locus_tag="VIJ44_03190"
     CDS             complement(729..2243)
                     /gene="lysS"
                     /locus_tag="VIJ44_03190"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEY5164937.1"
                     /translation="MADDGRAGDERDRRLAKVDALRAGGTDPYPVRFDRTHTAAELHD
                     HWAHLDPGAETTDVVRVAGRSMLLRRQGKLTFATLRDGTGTVQLFVSQNTIGDDAHAA
                     FDDLDLGDWIGAEGVVMKTKRGELSVKVQSFELLAKALRPLPSKWHGLSDVDTRFRMR
                     YVDLIANDDARRVFAIRFAVIAAIRDFLTQRGFVEVETPVLHTQPGGATARPFDTFHN
                     ALDIPLSLRVALELHLKRLLVAGYERVFEIGRVFRNEGVSTRHNPEFTMLEWYQAFAD
                     YTDMASLTEELVAHCARVATGSTTVTVDGQPIDLTPPWPRRPMLDLIREHTGESVHPT
                     MPVADLAAVCDRLGVPRDEGLGGGQLVMELYEKTVEPHLVGPVLVTDYPRDVSPLARR
                     RADDPALVERFEAVVLGRELANAFSELNDPIDQRRQFEAQARLAAGGDDEAHGVDEDY
                     LRALEYGLPPAGGVGIGVDRLVMLVAGVTSIREVILFPHLRPEAGETDEEGGTS"
     gene            complement(2283..3110)
                     /locus_tag="VIJ44_03195"
     CDS             complement(2283..3110)
                     /locus_tag="VIJ44_03195"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018102267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEY5164938.1"
                     /translation="MLLAIDSGNTQTVIGLFDGKRLADHWRIATVAERTADELALMIQ
                     QFLAFHGFAPFLVDAVGVPTPRAPATGADVDDAPINGVVLSSGVPRVTAELREMTDRY
                     FGFPALVLGPGVRTGMPILYDNPKEVGADRIANAVAAYDLYGGPSIVVDFGTANTIEA
                     ISEKGEYLGGAIFPGIEISMNALFERAAALGRVELVAPRHVIGKSTVESIQSGCVYGY
                     SGQVDALVDRFQAELGECAVVATGGLAEPIITHSRTVQHYEPWLTLAGLRIVFERNR"
     gene            complement(3110..3973)
                     /gene="nadC"
                     /locus_tag="VIJ44_03200"
     CDS             complement(3110..3973)
                     /gene="nadC"
                     /locus_tag="VIJ44_03200"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011814828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEY5164939.1"
                     /translation="MTDLDAPRHLVREAVARAINEDLGPLGDLTSALLPPHTDATGAI
                     TARAAGVLAGQACATETWAQLDTEVDVRWTAADGCALTAGTVVATIAGPLRSVLTGER
                     TALNFLCHLSGIATLTQRFVAEAGDQIRIWDTRKTTPGLRALEKAAVRAGGGVNHRGN
                     LSDMVLIKDNHLAGLSIDDAVRTARDRWPGRGIEVECEQLDQVHAAVAAGADLVMLDN
                     LTPDQARACIAAVRTGPRPDLPVEVSGGITLDTVAAYASTGADLISTSVITQSAPALD
                     LGLDLHDPDTR"
BASE COUNT          549 a         1221 c         1658 g          609 t
ORIGIN      
        1 gatccggccg ccgaggcgga cggcctgcca cgggctggtg gcgccgggcc cgacgacgtt
       61 cagtgggcct tcgaagccct gcaccagcat ctcgaccatt gcccgggccg cgtcgtcttg
      121 gtggaggagg gagaacggcg ggtcggcgag cgccgggacg gggacgacgg ggagtcgcag
      181 cacccgtccg agggggctcg ggatgtggga cccggcgacg acggcgtacc gcaacgcgcc
      241 gacgggcacg tcgtggcgtc ggccgatgcc gagggcgagg gactcgacct cgaggcaggt
      301 gcgaccgtac ggggtcgtcg gcgcgagggg ggcttcctcg tcggggacga gggggtggcc
      361 ccgcccccgc ccgtaaacct cgaggccgct gcgcaccgcg atgcgtccga ggtgcccggc
      421 gcgttgcgcg gcgccgaggg cgttgacggt gcacgcctcg gtgctcgccg cagcctcccg
      481 cgatcccatc cgggagccgg gttcgtagac gccgaagtgg gcgacggcgt ggggtgcgaa
      541 gtcggtgacg aacccgatga ggcggtcccg gtcccggggg tcgatccgct tgaacgtggc
      601 gcgtcggagc cgacggcggg gcggcatgaa gtcgaacccg gcgatctcgt cgacctctgg
      661 tcgcgtttcg aggagctggg cgacccgggt cccgagctct ccgcccatgc cggtgaccag
      721 gacacgcatc agctggttcc cccttcctcg tcagtctcgc ccgcttcggg gcgcaggtgg
      781 gggaacaaga tgacctctcg gatgctggtc acgccagcca cgagcatgac gaggcggtcg
      841 acgccgatcc cgacgccgcc ggccggtggg agcccgtact cgagggcccg gaggtagtcc
      901 tcgtcgacgc cgtgggcctc gtcgtcgccg ccggcggcga ggcgggcttg ggcctcgaac
      961 tggcggcgct ggtcgatggg gtcgttgagc tcgctgaagg cgttggcgag ctcgcggccg
     1021 aggacgacgg cctcgaaccg ttcgacgaga gcggggtcgt cggctcgacg gcgcgccagc
     1081 ggcgagacgt cgcgggggta gtcggtgacg agcaccggtc cgacgaggtg gggttcgacg
     1141 gtcttctcgt agagctccat gacgagctgg ccgccaccca ggccttcgtc gcgggggacg
     1201 ccgaggcggt cacagaccgc ggcgaggtcg gcgaccggca tggtggggtg caccgattcg
     1261 ccggtgtgtt cgcgaatgag gtccagcatc ggtcggcgcg gccacggcgg tgtgaggtcg
     1321 atcggttgcc cgtcgacggt gacggtggtg ctgccggtcg cgacgcgggc gcagtgggcg
     1381 acgagctctt cggtgaggga cgccatgtcg gtgtagtcgg cgaacgcctg gtaccactcg
     1441 agcatggtga actcggggtt gtgacgggtg gagacacctt cgttgcggaa gacgcggccg
     1501 atctcgaaga cgcgctcgta gccggcgacg aggaggcgtt tgaggtggag ctcgagcgcg
     1561 acgcggaggc tgagggggat gtcgagggcg ttgtggaacg tgtcgaacgg tcgtgcggtg
     1621 gcaccgccag gctgggtgtg gaggacgggg gtctccactt cgacgaatcc tcgctgggtg
     1681 aggaagtcac ggatggcggc gatgaccgcg aaccggatcg cgaagacccg gcgggcgtcg
     1741 tcgttggcga tgaggtcgac gtagcgcatc cggaaccggg tgtcgacatc gctgaggccg
     1801 tgccatttgc tggggagggg ccgcagcgcc ttggcgagca gctcgaagga ctggaccttg
     1861 accgacagct ctccccgttt ggtcttcatg acgacgcctt cggcgccgat ccagtcgccg
     1921 aggtcgaggt cgtcgaacgc ggcgtgggcg tcatcgccga tggtgttctg cgacacgaag
     1981 agctgcacgg tgccggtccc gtcgcgcagc gtcgcgaacg tgagcttgcc ttggcggcgt
     2041 agcagcatgc tccgtcccgc gacgcgcacg acgtcggtgg tctcggcacc ggggtcgagg
     2101 tgggcccagt ggtcgtggag ctcggcggcg gtgtgggtcc ggtcgaagcg caccggatac
     2161 gggtcggtgc cgccggcgcg gagcgcgtcg accttggcga ggcggcggtc gcgttcgtcg
     2221 ccggcgcgtc cgtcgtcagc catggtggtg atctggttcg agtggaccgc ggatggggcc
     2281 gctcatcggt tgcgctcgaa gacgatgcgt agccccgcga gggtgagcca cggctcgtag
     2341 tgctgcacgg ttcgggagtg ggtgatgatc ggctccgcga ggccgccggt ggcgacgacc
     2401 gcacactctc cgagctcggc ttggaaccgg tcgacgaggg cgtcgacctg gccgctgtac
     2461 ccgtagacgc agccggactg gatcgactcg acggtggact tgccgatgac gtggcggggg
     2521 gcgaccagct cgacgcgtcc gagggccgcg gcccgctcga agagggcgtt catggagatc
     2581 tcgatcccgg ggaagatcgc cccgccgagg tactcgccct tctcgctgat ggcctcgatg
     2641 gtgttcgcgg tcccgaagtc gacgacgatc gacggccccc cgtagaggtc gtaggcggcg
     2701 acagcgttgg cgatccggtc ggcgccgacc tctttggggt tgtcgtagag gatcggcatc
     2761 ccggtgcgga ccccgggccc gaggacgagg gccgggaacc cgaagtaacg atccgtcatc
     2821 tcgcgcagct cggcggtgac gcgggggacg ccggaggaga gcacgacgcc gttgatgggg
     2881 gcgtcgtcga cgtcggcgcc ggtggcgggg gcccgggggg tggggacacc aacggcgtcg
     2941 acgaggaacg gggcgaaccc gtggaaggcg aggaactgtt ggatcatgag ggccagctcg
     3001 tcggcggtcc gttcggcgac ggtggcgatg cgccagtggt cggcgagtcg cttgccgtcg
     3061 aagaggccga tgacggtctg ggtgttgccg gagtcgatgg cgaggagcat cagcgggtgt
     3121 cgggatcgtg gaggtcgagg ccgaggtcga gcgcgggcgc cgactgggtg atgacgctgg
     3181 tcgagatcag gtcggcgccg gtcgatgcgt acgccgcgac ggtgtcgagg gtgatgcctc
     3241 ccgagacctc gaccgggagg tcggggcggg ggccggtgcg caccgcggcg atgcacgcgc
     3301 gggcctggtc gggggtgagg ttgtcgagca tgacgagatc ggcgccggcc gcgaccgcgg
     3361 cgtggacctg atcgagctgc tcgcactcga cctcgatccc acggccaggc cagcggtccc
     3421 gagccgtcct gaccgcgtcg tcgatgctga ggccggcgag gtggttgtcc ttgatcagga
     3481 ccatgtcgga gaggttgccg cgatggttga cgccgccacc ggcgcgcacc gcggctttct
     3541 cgagcgcgcg caggcccggg gtggtcttgc gggtgtccca gatccgaatc tggtctccgg
     3601 cctcggcgac gaaccgttgg gtgagggtgg cgatccccga gaggtgacag aggaagttga
     3661 gcgcggtgcg ctcgccggtg aggacgctgc gcaacggtcc cgcgatggtg gcgaccaccg
     3721 tgcccgcggt gagggcgcac ccgtcggcgg cggtccagcg cacgtcgacc tcggtgtcga
     3781 gttgggccca ggtctcggtg gcgcaggcct ggcccgcgag gaccccggcg gcgcgggccg
     3841 tgatggcacc ggtggcgtcg gtgtggggtg ggaggagcgc gctggtgagg tccccgagcg
     3901 gtccgaggtc ctcgttgatg gcgcgcgcga cggcctcgcg caccaggtgc cggggcgcgt
     3961 cgaggtcggt catggggtgg cagcgaactc ggggaggtgg acgaactggg gggtcgcggt
     4021 gccggcgaac acgaagc
//
LOCUS       DATCCH010000158         4055 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_132725, whole genome shotgun sequence.
ACCESSION   DATCCH010000158 DATCCH010000000
VERSION     DATCCH010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132725"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="VIJ44_03205"
     CDS             <1..401
                     /locus_tag="VIJ44_03205"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY5164940.1"
                     /translation="LGLLEHQYGIIPDLGGTQRLPRLVGPGKALQLITTAARIDAEEA
                     ERIGLAEILVSEEDFETTAAELADTIAAQPPLAVRAAKRAVQAAGSGLTVREGLVVEA
                     EGQAVCLRSNDLGEAIAAFVEQRPPVYGGH"
     gene            445..1725
                     /gene="murA"
                     /locus_tag="VIJ44_03210"
     CDS             445..1725
                     /gene="murA"
                     /locus_tag="VIJ44_03210"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019876306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEY5164941.1"
                     /translation="MERYLVRPGAPLSGTARVSGMTKNAGLKQMAAALLAPGVTTLRN
                     MTPVADLDVMVELLGAIGATVERTGVDELRIDASNELIPEAPYEHVRRMRASINVLGP
                     LLARHGRARVAMPGGDNIGSRKLDMHFRALESMGAELDVQHGFIEARCNALASARVVF
                     EFPSVGATETVLTAAVLAKGESVIENAAREPEIVDLCTFLAKMGAHIDGAGTSTVHVE
                     GVEMLTATEHQIIGDRIEAGTLLFACAVAGGDIVVEGVPLAHLETVARKLGQMGMFVG
                     PAPGGIAASAEGRLDAVDVQTLPFPGFATDFMPLAVAALAVADGTAIVTENIFDNRFV
                     FIDELNRMGADIRTEGRHAVVRGVPRLSGAPVRVPDVRAGAALLLAGLAADGETVVHD
                     THHVERGYPDLPGHLAALGADVSRAPRDREDLRD"
     gene            1743..1973
                     /locus_tag="VIJ44_03215"
     CDS             1743..1973
                     /locus_tag="VIJ44_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HEY5164942.1"
                     /translation="METEVLEAIDAIRPALQADGGDIVFHSIDDAGVVHVELVGACGT
                     CPISTVTLKAGVERIIKDRVPGVSAVEALDEG"
     gene            1999..2916
                     /gene="meaB"
                     /locus_tag="VIJ44_03220"
     CDS             1999..2916
                     /gene="meaB"
                     /locus_tag="VIJ44_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017580889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEY5164943.1"
                     /translation="MVDGTRAGDRGAMARLLSLVESGGPAAAEALALVFPYTGRAYTI
                     GITGPPGSGKSTLTDRLVGRIRRAGDEVGVLAVDPTSPFTGGAILGDRIRMQDHSTDT
                     GVFIRSMATRGHLGGLALATPHAVRVLDAAGKPWVIIETVGVGQVEVEVAAAADTTIV
                     VVNPGWGDAVQANKAGLLEIADVFVVNKADRPGGDETVRDLRQMIEWSGERDWNPPIV
                     ETVATNGQGIDEVWDTIAAHRSHLETDGRLAARRAARLRDEIRSLVVERLGERAAALC
                     AGPGFDELLGRVESRDVDPHAAAATLLNQ"
     gene            2931..3710
                     /locus_tag="VIJ44_03225"
     CDS             2931..3710
                     /locus_tag="VIJ44_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY5164944.1"
                     /translation="MDEPLVRTEPQPDGITLLRLNRPPLNALSQALLGELAATAQSLA
                     ADPSVKAVVVTGNGRAFAAGADITEFGSSDAAHRIGTHFRAAFDAVAAIPRPVIAAIR
                     GVALGGGLELALACDLRVAADTARLGQPEILLGIIPGAGGTQRLPRLVGTARAKEMVW
                     SGRQLRADEAHSIGLVDRVAPADETESAALAWAATFATGAVTAMGAAKGAIDRGLDGP
                     LDAGLDLEHRAFTEVFRTDDAQEGVRSFLEHGPGHATFRGR"
     gene            complement(3707..>4055)
                     /locus_tag="VIJ44_03230"
     CDS             complement(3707..>4055)
                     /locus_tag="VIJ44_03230"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY5164945.1"
                     /translation="DALDIEFLTTSHRGVGTEFACVTKLGPLRTRDILVVTEWEPPSQ
                     MTIEHRGSVQGVGQFLLRAAADGTEFCWEETLRFPWWMGGPVGERVARPFFARLWRGN
                     LRRLRDRFEPEPG"
BASE COUNT          585 a         1493 c         1411 g          566 t
ORIGIN      
        1 gtctgggtct cctcgagcac cagtacggca tcatcccgga cctcggtggc acccaacgcc
       61 ttccgcgtct cgtcgggccg ggcaaggccc tccagctcat caccaccgcg gcgcgcatcg
      121 acgccgaaga agccgaacgg atcggcctcg ccgagatcct cgtctccgaa gaggacttcg
      181 agaccaccgc cgccgagctg gccgacacca tcgcggcgca gccaccgctc gcggtgcggg
      241 ccgcgaagcg agccgtgcaa gccgccgggt ccggcctcac cgtccgcgaa ggcctcgtgg
      301 tggaggccga ggggcaggcc gtctgcctcc gctccaacga cctgggggag gcgatcgcgg
      361 ctttcgtcga gcagcggcca ccggtctacg gaggccacta agcagagccg gtcgatcgcg
      421 ccgaggacga cgtaccatcc ggccatggag cgctatctcg tgcgacccgg cgcgcccttg
      481 tcggggaccg ctcgggtgtc gggcatgacc aagaatgccg gactgaagca gatggctgcc
      541 gcgctgctcg cccccggcgt caccacgctg cgcaatatga ctccggtcgc ggatctcgac
      601 gtcatggtcg agctgctcgg tgccatcggc gcgaccgtgg agcgcaccgg cgtcgacgag
      661 cttcggatcg acgcgtcgaa cgagctgatc cccgaggccc cctacgagca cgtgcggcgc
      721 atgcgggcgt cgatcaatgt gctcggccca ctcctggccc gccacggccg ggcccgggtc
      781 gcgatgccgg gcggcgacaa catcgggagc cgcaagctcg acatgcactt ccgcgccctc
      841 gagtcaatgg gcgccgagct cgacgtccaa catggcttca tcgaggcgcg ctgcaacgcg
      901 ctcgcgtcgg cccgcgtcgt cttcgagttc ccgtcggtgg gggcgaccga gacggtgctg
      961 accgcggcgg tgctcgccaa gggcgagtcg gtcatcgaga acgcggctcg cgagcccgag
     1021 atcgtcgacc tgtgcacctt cctcgccaag atgggagccc acatcgacgg ggcgggcacc
     1081 tcgaccgtgc acgtcgaagg ggtcgagatg ctcaccgcga ccgagcatca gatcatcggc
     1141 gaccggatcg aggccggcac gctgctgttc gcctgcgccg tcgccggcgg cgacatcgtc
     1201 gtcgaaggtg tcccgctggc ccacctcgag accgtcgccc gcaagctcgg gcagatgggc
     1261 atgttcgtcg ggccggcccc gggagggatc gcggcgagcg ccgaagggcg cctcgacgcg
     1321 gtcgacgttc agacccttcc cttccccggc ttcgccaccg acttcatgcc gctcgctgtc
     1381 gccgcgctcg ccgttgccga cggcacggcc atcgtcaccg agaacatctt cgacaaccgc
     1441 ttcgtgttca tcgacgaact caaccgcatg ggcgcagaca tccgcacgga aggccgccac
     1501 gccgtcgtcc ggggcgtgcc ccgcctctcg ggggcaccgg tgcgggtccc ggacgtgcgg
     1561 gccggcgcgg cgttgctgct cgccggcctc gccgccgacg gtgagaccgt cgtccacgac
     1621 acccaccacg tcgagcgggg ctaccccgac cttcccggcc acctggcggc tctgggcgcc
     1681 gacgtgtccc gggcgccccg agaccgggaa gacctgcgag actgagggtg ttgaacaccg
     1741 tgatggagac tgaggttctc gaagcgatcg acgccatccg gccggctttg caggccgacg
     1801 gcggcgacat cgtcttccac tcgatcgacg acgccggcgt cgtccacgtc gagctggtcg
     1861 gcgcctgcgg gacctgtccc atctcgacgg tgacgctgaa ggccggtgtg gagcggatca
     1921 tcaaagaccg ggtgcccggc gtgagcgccg tcgaggccct cgacgaaggc tgacggagcc
     1981 gccgccgatc cggcggcgct ggtcgacggg acgcgtgccg gggatcgggg cgccatggcg
     2041 cgtctgttgt ccctcgtcga gtcggggggc cccgccgcgg ccgaagccct ggcgctggtc
     2101 ttcccgtaca ccggccgggc ctacacgatc gggataaccg ggccgcccgg gtcagggaag
     2161 tcgacgctca ccgaccggct cgtcggacgc atccgccgcg ccggcgacga ggtcggtgtc
     2221 ctcgcggtgg accccaccag cccgttcacc ggaggtgcga tcctcggcga ccggatccgc
     2281 atgcaggacc actccaccga caccggcgtg ttcatccggt cgatggccac ccggggccac
     2341 ctcgggggcc tggcgttggc caccccgcac gccgtccggg tccttgacgc ggccgggaag
     2401 ccgtgggtga tcatcgagac cgttggcgtc ggccaagtcg aggtcgaggt cgccgccgcc
     2461 gccgacacga ccatcgtggt ggtcaatccc ggctggggcg acgcggtgca ggccaacaag
     2521 gccgggctgc tcgagatcgc cgacgtcttc gtcgtcaaca aggccgaccg cccggggggt
     2581 gacgaaacgg tgcgtgacct gcgccagatg atcgagtggt cgggcgaacg ggactggaac
     2641 ccaccgatcg tcgagaccgt cgccaccaac gggcaaggca tcgacgaggt gtgggacacc
     2701 atcgccgcgc accgctccca tctcgagacc gacggacggc tggcggcgcg ccgcgcggcc
     2761 cggctgcgcg acgagatccg ctccctggtc gttgagcggc tgggcgaacg cgccgcggcc
     2821 ctctgcgccg ggcccggctt cgacgagctc ctcggtcggg tggaatctcg cgacgtcgac
     2881 ccgcacgccg ctgctgccac gctgctcaac caataatagc ctgaccggcc gtggacgagc
     2941 cgttggtgcg caccgagccg cagccggacg ggatcaccct gcttcgcctc aaccgacctc
     3001 ccttgaacgc cttgtcccaa gcgctgctcg gcgagctcgc ggcgaccgca cagtcgctcg
     3061 ccgccgatcc gagcgtcaaa gcggttgtgg tcaccggcaa cggccgggcg ttcgcggccg
     3121 gcgcggacat caccgagttc ggctcgtccg acgccgcgca ccggatcgga acgcattttc
     3181 gggccgcgtt cgatgccgtc gccgccattc ccagaccggt gatcgccgcc atccgtggcg
     3241 tcgcgctcgg cggtggcctc gagctcgccc tggcgtgtga cctgcgcgtc gccgccgaca
     3301 cggctcggct cggccagcct gagatcctgc tcggcatcat ccccggcgcc ggggggacgc
     3361 aacggctgcc gcgccttgtc gggacggccc gggcgaagga gatggtgtgg agcggccggc
     3421 agctgcgagc cgacgaggcc cattcgatcg gtctcgtcga ccgggtcgcg cccgccgacg
     3481 agaccgagtc cgccgcgctg gcctgggccg cgacgttcgc gaccggtgcc gtcaccgcga
     3541 tgggcgcggc caagggcgcc atcgaccgcg ggctcgacgg gcccctcgac gccggcctcg
     3601 acctcgagca ccgcgcgttc accgaggtgt tccgcaccga cgacgcccag gagggcgtcc
     3661 gctccttctt ggagcacggc cccggccacg ccaccttccg gggccgctaa cccggttcgg
     3721 gttcgaaccg gtctctgagc cgccgcagat tgccgcgcca cagccgggcg aagaacggcc
     3781 gtgcgacgcg ctcaccgacc ggtccgccca tccaccacgg gaagcgcaac gtctcctccc
     3841 agcagaactc ggtgccgtcg gcggcggccc ggagcaggaa ctggccgacg ccctggacgc
     3901 tgccccgatg ctcgatcgtc atctgcgacg gcggttccca ttcggtgacg acgaggatgt
     3961 cgcgggttcg cagcgggccg agcttcgtca cgcacgcgaa ctcggtgccg acgccgcggt
     4021 gcgacgtggt gaggaactcg atgtccagcg cgtcg
//
LOCUS       DATCCH010000159         2083 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_423242, whole genome shotgun sequence.
ACCESSION   DATCCH010000159 DATCCH010000000
VERSION     DATCCH010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423242"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(298..>2083)
                     /locus_tag="VIJ44_03235"
     CDS             complement(298..>2083)
                     /locus_tag="VIJ44_03235"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5164946.1"
                     /translation="TAYKMPNNANGWDPACVPHYVALTDQVHEHETLAFIQLTHSGAM
                     MLGNWSKQVALAPSSVPDYFETPKPMDRRDIAEMIDYHARCARNAVVGGFDGIEIQSA
                     HGYLLHQFLSPKFNERTDEYGGSLENRMRFGAEVLAAVREAAGDRVAVGLRLAGDDEQ
                     HHGPGLTAADAAAVAARYEELGLVDFVNVSVGIGGVGMVRTNYAPHGFGVYAARAVKQ
                     AVRDTPVFTVHRIVAPDEADGIVERGDADAVTLVRALIADPEWVAKARDGRSETIRKC
                     TGINQSCYGNLLQSMPINCVQNPAVGREDDLGLGTLTPASVPKRLVVVGGGPAGLEAA
                     WVAATRGHHVTLLESGDELGGAIRWAQRLPGRSEIADLADWRIGECQRRGVEMRTGVH
                     ASAASVLALEPDAVIVATGGHATKDGRSAYHPMPVPGSEQEWVLDHVEALRRAVDDTA
                     ALGPRVMILDVVGHVQGIGLAELLASQGHETTVVCPLPVLLACDSETQAAILPRAVQA
                     GVTWRPSTGLVGIGDHTVTLVDVLSRRVETIAGVDTVAICTNGVSNDELFHALDGQVA
                     DIVRVGDAVAVRPVDRAVFDGHQAARAL"
BASE COUNT          297 a          738 c          714 g          334 t
ORIGIN      
        1 ccgcgagctt cgtcagcggt caggcggaca ccgtcgccgg cggcgcccac gtctgacctg
       61 gccgtcgtcc ccagagccgg ccagtttgat ttggcgatcg tgggtgagaa cgggccggtc
      121 acgctccaac gggatgccgg ctccgtccga tcggtcctgg tgagttgtcg gatcgaactc
      181 ggggcatccc gtcagtggcc atgatgtacg tgacgtcagc cgagggaagc gcgccctcct
      241 gggacgagcc tcaccccggg ctctgacccc tgcgtgaccc gcgaccgggg cctagttcta
      301 gagcgcccgg gccgcttgat gaccgtcaaa gaccgcccgg tcgacggggc gcaccgccac
      361 cgcgtcgccg acccgcacga tgtcggccac ctgaccgtcg agcgcgtgga acagctcgtc
      421 gtttgacacc ccgttggtgc agatcgcgac ggtgtcgacc ccagcgatgg tctcgacgcg
      481 acgggacaag acgtcgacga gcgtcacggt gtggtcgccg atgccgacca ggccggtgct
      541 cggccgccag gtcacaccgg cctgcaccgc ccggggaagg atcgccgctt gggtctcact
      601 gtcgcaggcc agcagcactg gcagcggaca gaccaccgtc gtttcatggc cctgtgacgc
      661 gagcagctcc gcgagcccga ttccttggac gtgaccgacg acatcgagaa tcatcacccg
      721 tggtccgagc gccgcggtgt cgtcgacggc gcggcgcagc gcctcgacat ggtcgaggac
      781 ccattcctgc tccgaccctg gaaccggcat cggatggtaa gccgaacgcc cgtccttggt
      841 cgcgtgtcca ccggtcgcga cgatgacggc atcgggctca agcgcgagga ccgacgcggc
      901 gctggcgtgc actccggtgc gcatttcaac ccctcgccgc tggcattcac cgatccgcca
      961 gtcggccagg tcggcgatct cggaccggcc gggaagtcgc tgcgcccacc ggatggcgcc
     1021 acctagctcg tcgccgctct cgaggagcgt gacgtgatga ccgcgtgtcg ccgcgaccca
     1081 cgctgcctcg agaccggccg gtcctccccc cacgacgacg agccgcttgg gaaccgaggc
     1141 cggcgtcagc gtgcccagcc ccaggtcgtc ctcgcgtccg accgcggggt tctggacgca
     1201 gttgatcggc atcgactgga gcaggttccc gtagcacgac tggttgatcc cggtgcactt
     1261 gcggatggtc tcgctccgcc cgtcgcgggc cttcgccacc cactccgggt cggcgatgag
     1321 cgcgcggacg agggtgacgg cgtcggcgtc gccccgctcg acgatcccgt cggcctcgtc
     1381 gggggccacg attcggtgca cggtgaacac cggcgtgtcc cgtaccgcct gcttcacggc
     1441 tcgggccgcg tacacaccga agccgtgcgg tgcgtagttc gtccggacca tcccaacacc
     1501 gccgatcccg acgctgacgt tcacgaagtc caccagcccg agctcctcgt agcgggcggc
     1561 gaccgcggcg gcgtccgccg cggtcagccc cggcccgtgg tgctgctcgt cgtcgccggc
     1621 gagccgcaac ccgaccgcga cccggtcccc ggccgcctcc cgcaccgccg cgagcacctc
     1681 ggcgccgaag cgcatccggt tctcgaggct gccgccgtac tcgtcggtgc gctcgttgaa
     1741 cttgggtgag aggaactggt gcaagaggta gccgtgggcg ctctggatct cgattccgtc
     1801 gaagccaccg acgacggcgt tgcgggcaca tcgggcgtgg tagtcgatca tctcggcgat
     1861 gtctcgccgg tccatgggct tgggggtctc gaagtagtcg ggcaccgacg agggcgcgag
     1921 cgcgacctgc ttcgaccagt tgcccagcat catcgcgccg ctgtgggtca gctggatgaa
     1981 ggcgagcgtc tcgtgttcat ggacctggtc ggtcagcgcg acgtagtgcg ggacgcaggc
     2041 tgggtcccag ccgttggcgt tgttgggcat cttgtaggca gtg
//
LOCUS       DATCCH010000160         2397 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_515646, whole genome shotgun sequence.
ACCESSION   DATCCH010000160 DATCCH010000000
VERSION     DATCCH010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515646"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="VIJ44_03240"
     CDS             <1..437
                     /locus_tag="VIJ44_03240"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEY5164947.1"
                     /translation="NRPSPDTTVEAPLPAWAPLAVDPPVIFVGGPVQPEAAIGLARQA
                     ERGVPDGFAPLSDELGTVDLERDPAAVAPRIDRLRVFAGYSGWGPGQLEHELEADGWF
                     VVDAEPSDLWTAEPETLWRTVLRRQRGKERLFAEYPLDASLN"
     gene            complement(430..1797)
                     /locus_tag="VIJ44_03245"
     CDS             complement(430..1797)
                     /locus_tag="VIJ44_03245"
                     /inference="COORDINATES: protein motif:HMM:NF021524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HEY5164948.1"
                     /translation="MTPGVRREKLRATLWLLPALLVLAAALLFGVTYTLDRLAHHGDL
                     SFPGWIDTGDAGAARTILSAIAAAVITVAGVVFSVTIVAFTLASTQFGPRILRNFLRD
                     RGTQTTLGTFVATFVYSVLALGSVSSRGAHPFVPHLSTAVALLLMLIDVVVLIYFIHH
                     VTTSIQLNEVVFSIAQDLGRAVDALEMDTPSPATAPSPNLDELRQGLQRDGVGVPATT
                     SGYLQAIGHRRLVEIARDNNAVIELTFRPGHFVTIGQPLAKVWPATAEPAVAQLLERN
                     HVTGPHRTLQQDLQFAIDQLVEIAIRALSPAVNDTFTALSCIDWLGDGLTRLSGRPLP
                     SGVIRDADGAVRLLVPPLDYERLVNRSFDQIRQAGRGLPAVGIRQLDTLSRIAEQTQS
                     DAQRRVIARQAAMLLREGTDPDAVPEALDRADLERRYDRVERALARTGRSSARQETKP
                     VEFTS"
     gene            complement(1964..>2397)
                     /locus_tag="VIJ44_03250"
     CDS             complement(1964..>2397)
                     /locus_tag="VIJ44_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009807184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164949.1"
                     /translation="AAGLVNPLQGEGIAQAMGSGRAAAEAVLAGPTHAAERYRRYLSM
                     RDGPYQSATAPLHAALVPHPVVVSTLARTVTAPGLGRLIAPGWSIFWNDLLDDAAPGS
                     PRAVARVATGLARAITVGGRTRRWFRTTFGPARADPNHRGQ"
BASE COUNT          359 a          810 c          867 g          361 t
ORIGIN      
        1 tgaaccggcc gtctcccgac acgaccgtcg aggccccgct cccggcgtgg gctccactcg
       61 cggtcgaccc cccggtcatc ttcgtcggtg gcccagtgca gcccgaagcc gcgatcggtc
      121 tcgcccgcca ggccgaacga ggcgtccccg acggtttcgc gccgctgtcc gacgagctcg
      181 gcaccgtcga cctcgaacgc gaccccgcgg cggtcgcacc ccgcatcgac cggctccgcg
      241 tcttcgccgg ctactccgga tggggacctg gccagctcga gcatgagctc gaagccgatg
      301 ggtggttcgt cgtcgatgcg gagccgtccg atctctggac cgccgagccc gagacgctct
      361 ggcgtaccgt gctgcgtcgt cagcgtggca aggagcggct gttcgccgaa tacccgctcg
      421 acgccagcct caactagtga actccactgg cttcgtctcc tggcgggccg atgaccgtcc
      481 ggtccgggcc agcgcccgtt cgacccggtc gtagcggcgc tcgagatccg ctcgatccag
      541 ggcctcgggg accgcgtcag gatcggtgcc ctcacgtaac agcatcgcgg cctgtcgcgc
      601 gatgacccgt cgctgcgcgt cgctttgggt ctgctcggcg atcctcgaca gcgtgtcgag
      661 ctggcggatg ccgactgccg gcaggccccg tccggcctgg cggatctgat cgaaggaccg
      721 gttgacgagc cgctcgtagt cgaggggcgg caccaagagg cggaccgccc cgtcggcgtc
      781 acggatgacg ccgctgggca ggggtcgccc ggagaggcgg gtcagaccgt ccccgagcca
      841 gtcgatgcag ctgagggcgg tgaaggtgtc gttgaccgca ggggacaggg ctcggatcgc
      901 gatctccacc agctggtcga tcgcgaactg caggtcttgt tgcagcgtgc ggtgcggccc
      961 ggtcacgtgg ttccgctcca gcagttgggc gacggccggc tccgcggtgg cgggccacac
     1021 cttggccaag ggttgaccga tggtcacgaa gtggccggga cgaaaggtga gctcgatcac
     1081 cgcgttgttg tctctcgcga tttcgaccag ccggcgatgt ccgatcgcct gcaagtaccc
     1141 gctcgtcgtg gccgggaccc cgacgccgtc gcgctgcaaa ccttggcgga gctcgtccag
     1201 gttcggagac ggggccgtgg cgggtgaggg tgtgtccatc tcgagcgcgt cgacggcgcg
     1261 tcctagatcc tgggcgattg agaagaccac ctcgttcagc tggatcgatg tggtgacgtg
     1321 atggatgaag tagatgagca ccacgacgtc gatcagcatc agcagcagcg cgaccgcggt
     1381 cgacaggtga ggaacaaacg ggtgagcacc gcgactcgac accgacccga gcgcgaggac
     1441 gctgtacacg aacgtcgcga cgaatgtgcc gagggttgtc tgggtgccgc gatcgcgcag
     1501 gaagttgcgc aggatgcggg ggccgaactg ggttgacgcc agcgtgaacg cgacgatggt
     1561 caccgagaac acgacgccgg cgacggtgat gacggccgcg gcgatcgcgc tgaggatcgt
     1621 ccgtgccgcg ccggcgtccc cggtgtcgat ccagccgggg aaggacaggt cgccgtggtg
     1681 ggcgagtcgg tccagggtgt acgtcacccc gaagagcagc gcggccgcga ggacgagcaa
     1741 cgcggggagc agccacaggg tcgcccgcag cttctcgcgt cgaacgccag gcgtcatcgc
     1801 cacgccctcg gcgatcctgg gagatctagg aggagtacgc gcaccgagga ggtccttccg
     1861 acgactattc cgcggccgac cagtcttccc ggcgttccga ctcccatgat agatggggct
     1921 gcctcgactc cagtgtctcg acggtcgttc ggcccgcgga gggtcactgg ccccggtggt
     1981 tcgggtcagc gcgggccggg ccgaacgtcg tgcggaacca ccgccgggta cgtccgccga
     2041 cggtgatggc ccgagccagg cctgtcgcca cccgcgccac ggcccgcggc gatcccgggg
     2101 cggcgtcgtc gagcaggtcg ttccagaaga tcgaccaccc cggggcgatc agacgaccga
     2161 gcccgggcgc ggtcacggtt cgcgccagcg tcgacaccac cacggggtgg ggaaccagcg
     2221 cggcgtggag gggtgccgtg gccgactggt acggcccgtc gcgcatcgag aggtaccgcc
     2281 ggtaccgttc cgcggcgtgg gtcggtccgg ccagcaccgc ctcggcggcg gcccgaccgc
     2341 tgcccatggc ctgggcgatc ccttctcctt ggagcgggtt cacgagacca gcggcgt
//
LOCUS       DATCCH010000161         3456 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_556462, whole genome shotgun sequence.
ACCESSION   DATCCH010000161 DATCCH010000000
VERSION     DATCCH010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556462"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..1105
                     /locus_tag="VIJ44_03255"
     CDS             26..1105
                     /locus_tag="VIJ44_03255"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY5164950.1"
                     /translation="MRDDVIGVGLVGCGMIGQIHADGLRKLVEDGDIRAVAAADPSEE
                     ARGAANRNCPFARLHPDAAAVIDDPEVDAVMITAPTSAHRELVLATVAAGKPLFCEKP
                     LAPTFDVVRELVAAVAGIPAQVGFHSRFHPLHNHLRRLIGSGEVGAAMGYTLRDDQYW
                     PTGDVVAGHSSWRSRRADAGGGALLEHSIHSVDILTWLFGPPVRVSAVSRRVFGYDVE
                     DVAALVIEHRSGVVGTLLTIFNGVRGREERRLETFFENAAVEVTTNFLVGAPEDSFLI
                     QVADQPAERLDLAALRDRQFQALGITRDDVVFYTYVEDRAWVQAIRAGRPPSPGFRDA
                     LIAHAVVDAAYRAADLGTPVAVEDG"
     gene            complement(1137..1322)
                     /locus_tag="VIJ44_03260"
     CDS             complement(1137..1322)
                     /locus_tag="VIJ44_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164951.1"
                     /translation="MQLIAVQWGRLRPGVTILRADGRLERAATVEHQDRTTLVWFDDG
                     RLERHDDSDRAQVLTRA"
     gene            1711..1860
                     /locus_tag="VIJ44_03265"
     CDS             1711..1860
                     /locus_tag="VIJ44_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003405313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Trk"
                     /protein_id="HEY5164952.1"
                     /translation="MADLWVVLTVLSFFALCVLLIRGCDRIIGSDEASELSVETERDP
                     VGTPR"
     gene            1857..1946
                     /gene="kdpF"
                     /locus_tag="VIJ44_03270"
     CDS             1857..1946
                     /gene="kdpF"
                     /locus_tag="VIJ44_03270"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HEY5164953.1"
                     /translation="MTGENIAGLVLALLLTLYLVAALLFPERF"
     gene            1946..>3456
                     /gene="kdpA"
                     /locus_tag="VIJ44_03275"
     CDS             1946..>3456
                     /gene="kdpA"
                     /locus_tag="VIJ44_03275"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015627091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HEY5164954.1"
                     /translation="MTLAGWGQLIALVVLLGATAPLLGRYIAGVYEGAPSKLDRVFGP
                     VERLIYRSCRVDPEREQRWNVYALSLVAFSAVGVLLLYVMQRIQSNLPFNPTGMVNVT
                     PALSFNTAISFVTNTNWQSYAGETTLSHLTQMVGLTVQNFVSAAAGMAVMVALIRGLS
                     RAGQRTIGNFWVDLVRTIFRILLPISILFAVILLACGVIQNTHGFTTVHTVAGTTQKI
                     PGGPVASQESIKMLGSNGGGFFNVNSAHPFENPTKLSDFLELYSILLIPFALAFTFGR
                     MVKDKKQGYAVVAVMFTLWIAASVAVVYLEAGGNPKLNTGGVTQAVTQTSPGGNLEGK
                     DVRNGVVASGIWGASTTDTSNGSVNSMHDSFTPTAGLVEMGNMKLGEVSPGGVGVGLN
                     GLLVFAILAVFIAGLMVGRTPEYLGKKIQAPEVKLVAIYILVMPVIVLGFVAGSTFVG
                     SVNTSMLNSGSHGFSELLYAFTSAGNNNGSAFAGLNANTSWLNTTLAITMLFGR"
BASE COUNT          523 a         1186 c         1133 g          614 t
ORIGIN      
        1 ggtgtggaga acgaggggag gtcgggtgag agacgacgtc atcggggtgg ggctcgtcgg
       61 ctgcgggatg atcggccaga tccacgccga cgggctccgc aagctcgtcg aggatggtga
      121 tatccgcgcc gtcgcggcgg cggacccgtc cgaggaggcc cgaggcgcgg cgaatcgcaa
      181 ctgtccgttc gcgcggttgc atcccgacgc cgccgcggtc attgacgatc ccgaggtcga
      241 cgcggtgatg atcaccgcac cgacctcggc gcaccgtgag ctcgtgctcg cgaccgtcgc
      301 cgccggcaaa cccttgttct gtgagaagcc cctcgcgcca accttcgacg ttgttcgcga
      361 gctggtcgcc gccgtcgccg ggatcccggc ccaggtgggc ttccactcgc ggtttcaccc
      421 cctgcacaac cacctgcggc gcctcatcgg ctccggcgag gtcggcgcgg cgatgggtta
      481 caccctccgc gacgaccagt actggccgac tggggacgtc gtggccgggc acagctcgtg
      541 gcggtcccgg cgcgccgacg cgggcggcgg cgcgctgctc gagcattcga tccacagcgt
      601 cgacatcctc acctggctgt tcggtccacc ggttcgggtg tcggcggtca gccgccgggt
      661 gttcggctac gacgtcgagg atgtcgcggc gctggtcatc gaacaccgct cgggtgtcgt
      721 tgggacgctg ctgaccatct tcaacggggt gcgggggcgc gaggaacgac gacttgagac
      781 cttcttcgag aacgctgccg tcgaggtcac caccaacttc ctcgtcggtg cccccgagga
      841 cagcttcctc atccaagtcg ccgaccagcc cgcggagcga ctcgacctgg cagcgctgcg
      901 cgaccgccag ttccaggccc tcggcatcac ccgcgacgac gtcgtcttct acacctatgt
      961 cgaggaccgg gcctgggttc aggccatccg ggccgggcgg ccaccgtcgc cagggttccg
     1021 ggacgcgctt atcgcgcacg ccgtcgtcga cgccgcgtac cgcgccgccg accttgggac
     1081 ccccgttgcg gttgaggacg gctgagcaca tcccctcgac cgggcctggc ccagggtcag
     1141 gcgcgggtca ggacctgggc ccggtcgctg tcgtcgtggc gttcgaggcg accgtcgtcg
     1201 aaccagacca gggtggtgcg gtcttggtgc tcgacggtcg ccgcccgctc gagtcgcccg
     1261 tcggcgcgca ggatcgtgac cccagggcgg aggcgccccc actggacggc gatgagctgc
     1321 atcgacacga cggtctcttt ctcgtctcga ctgtgaccta cgacacccgg gccctcgagg
     1381 agcgacgaac cccaggcgtg gttctcctac cggtatagcg gtgcgcggcc cgtttttgat
     1441 gtcatcggag ccggctcgag cgccgcgtcc ctccgcgccg gggtggcgtc acgccgagcc
     1501 gatggtggcg gctcggcgac ccctcgatgt gttagcggtg cattaaggca aacggtcagg
     1561 gcgctaagaa cccgtcaggg cacttccccc ccgaccgggt ttcgcgtaca ccaacgttca
     1621 tgcgcgccac gtctcgcacc gattgccgcc aggggatcca ccccgcccac ctgaccgctg
     1681 gccgttctga ccgattcgcg aaggagaacc atggctgacc tctgggtggt cctcaccgtg
     1741 ctttcgttct tcgcgctgtg tgtgctcctg atccggggct gtgaccgcat catcgggagc
     1801 gacgaggcgt cggagctttc cgtcgagacg gagcgcgacc cggtcgggac gccgcggtga
     1861 ccggtgagaa catcgccggc ctcgtcctcg ccctgctgct gacgctctat ctcgtggcgg
     1921 ccctgctctt ccccgagcgt ttctgatgac cctggccggc tggggccagc tcatcgcgct
     1981 cgtggtactc ctcggggcca cggcgccgct gctcggacgc tacatcgcgg gcgtctacga
     2041 gggtgcgccc tcgaagctgg atcgcgtgtt cggcccggtg gaacgactca tctaccggtc
     2101 gtgtcgggtc gatccggaac gtgagcagcg ctggaacgtc tatgcgctct cgctggtggc
     2161 gttcagcgcc gtgggggtac tgctgctcta tgtgatgcag cgcatccaga gcaacttgcc
     2221 gttcaatccg accgggatgg tcaacgtcac cccggcgttg tcgttcaaca ccgcgatcag
     2281 cttcgtcacg aacaccaact ggcagagcta cgccggcgag accactctga gccacctcac
     2341 ccagatggtt gggctcacgg tccagaactt cgtgtccgcc gccgcgggca tggcagtgat
     2401 ggtggcactc atccgcgggc tgtcacgggc cgggcagcgc acgatcggca acttctgggt
     2461 cgatctggtc cgcaccatct tccggatcct cttgccgatc agcatcctct tcgcggtcat
     2521 cctgctcgcc tgcggcgtca tccagaacac ccacgggttc accaccgttc acaccgttgc
     2581 cggcacgacc cagaagatcc ctggcggccc ggtggcgagc caagagtcga tcaagatgct
     2641 cgggtcgaac ggcggagggt tcttcaacgt caactcggcg catccgttcg agaaccccac
     2701 caagctcagc gacttcctcg agctgtactc gatcctcctg atcccgttcg ccctggcctt
     2761 cacgttcggt cgcatggtca aggacaagaa gcagggctac gccgtcgtcg cggtcatgtt
     2821 cacgttgtgg atcgccgcga gcgtcgccgt cgtgtacctc gaggcgggcg ggaaccccaa
     2881 gctcaacacc ggaggcgtca cccaagccgt gacccagacc tctcccggtg ggaacctcga
     2941 aggcaaggac gtccgaaacg gcgtcgtcgc ctcgggaatc tggggggcct cgaccaccga
     3001 cacgtccaac gggtccgtca actcgatgca cgacagcttc acgcccaccg ccgggctggt
     3061 ggagatggga aacatgaagc tcggcgaagt cagtcccggc ggtgtcggcg tcgggctcaa
     3121 cggcctgctc gtcttcgcga tcctcgcggt gttcatcgca ggactgatgg tgggtcgaac
     3181 cccggagtat ctcgggaaga agatccaagc ccccgaggtc aagctcgtcg cgatctatat
     3241 cctcgtgatg ccggtcatcg tgctggggtt cgtggccggg tcgacgttcg tcgggtcggt
     3301 caacacctcg atgctcaact ccgggtcaca cggattcagt gagctgctgt acgcgttcac
     3361 ctcggcgggg aacaacaacg gctccgcgtt cgcggggctg aacgccaaca cgtcgtggct
     3421 caacacgacg ttggccatca cgatgctctt cggccg
//
LOCUS       DATCCH010000162         2218 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_307661, whole genome shotgun sequence.
ACCESSION   DATCCH010000162 DATCCH010000000
VERSION     DATCCH010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307661"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VIJ44_03280"
     CDS             complement(<1..304)
                     /locus_tag="VIJ44_03280"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEY5164955.1"
                     /translation="MTEREVLLTGIGGQGVQLAAQVLARAAVLEARHVLLFGVYGGAM
                     RGMNTDGTVVIGDEPIHSPPLLSRTWSAVAMHDRYWGPVRPKVRDGGVVVVNDSTFG"
     gene            complement(301..927)
                     /locus_tag="VIJ44_03285"
     CDS             complement(301..927)
                     /locus_tag="VIJ44_03285"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY5164956.1"
                     /translation="MQNVIPVERAVAARTLQDRADAYADEVRRLVDAAYAVMRRTGSV
                     DPRVADIVETAALSNQAFYRHFRGKDELLLAVLDDGQRRLVATLERRMARATRGAGKV
                     QAWIEGVLEQARNAEAAANTRPFALNGARLTERYPDATASQRDRVVAPLRDAVEAAGG
                     DRDRDADVIYYLTMGRAEDAIARGTAPTDADVAHTVTFALRAIGATAR"
     gene            complement(969..1412)
                     /locus_tag="VIJ44_03290"
     CDS             complement(969..1412)
                     /locus_tag="VIJ44_03290"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY5164957.1"
                     /translation="MGNLDVAKTVVDLLEAQRFDQARAMMTDDFSFSGPVPEPIDADG
                     FIGLMQALSAGLPNWSYRARDFRAEGPDRVTCTVTVGGTHTETLSLPPLGVSNLAPTG
                     IEARNPDETIHLTVRGDQIANLHADVPPDGGVAGIVRQLGVDVPG"
     gene            1581..>2218
                     /locus_tag="VIJ44_03295"
     CDS             1581..>2218
                     /locus_tag="VIJ44_03295"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY5164958.1"
                     /translation="MALELDLRAVSAERARRYVADGSWTDETLGTILGAGLVEGRDLT
                     LAIHSETRPWRGTFADGYDLACRVAGGLRAVGVRPGDPVAFQLPNWVEAAATFWAIAL
                     LGAVPVPIVHFYGPKEVAFILEQSRAQVLVTADQFGRLDYLANLDTFRPRLDGLREVF
                     VVGERIAAGTRPFEDLAGADPVDGPVATDPSAPALIAYTSGTTADPKGVVHS"
BASE COUNT          298 a          785 c          804 g          331 t
ORIGIN      
        1 caccgaaggt gctgtcattt acgacgacga caccgccgtc tcggaccttg gggcggaccg
       61 gtccccaata gcggtcgtgc atcgcgacgg ccgaccaggt acgcgacaac aacggcgggg
      121 agtggatcgg ctcgtcgccg atcacgacgg tcccgtcggt gttcatcccc cgcatcgctc
      181 cgccgtacac gccgaacagc agaacgtggc gggcttcgag caccgcggca cgagcgagga
      241 cctgggccgc gagctggacc ccttggcccc cgattccggt gaggaggacc tcccgctcgg
      301 tcatcgggcc gtcgctccga tggcccgcag ggcgaaggtc acggtgtggg cgacgtcggc
      361 gtccgtcggc gccgtgcccc gggcgatcgc gtcctcggcg cgacccatgg tgaggtagta
      421 gatgacgtcc gcgtcgcggt cccggtcgcc cccggcggcc tcgacggcgt cgcggagggg
      481 cgccaccacc cggtcccgtt ggctggcggt ggcgtcgggg taacgctcgg tgagccgggc
      541 gccgttcaac gcgaagggtc gggtgttggc cgcggcctcg gcgtttcggg cctgctccag
      601 gaccccttcg atccacgcct ggacctttcc tgcgccccga gtcgctcgcg ccatgcgccg
      661 ctcgagcgtg gcgacgaggc ggcgctgacc gtcgtcgagc accgcgagga gcagctcgtc
      721 cttgccgcgg aagtgccggt agaacgcctg gtttgagagc gccgcggtct ccacgatgtc
      781 ggcgacccgg ggatcgaccg acccggtgcg gcgcatcacc gcgtacgccg cgtcgacgag
      841 gcgccggacc tcgtcggcgt aggcgtccgc ccggtcctgg agggtccggg cggcgactgc
      901 ccgctcgacc ggaatgacgt tctgcacgtc cagaacagta ttccggcccg cggggggccg
      961 gcaaacgctc agcccgggac gtccaccccg agctggcgca cgatgcccgc gacgcccccg
     1021 tcgggcggga catcggcgtg gaggttggcg atctggtccc cccgcacggt gagatggatg
     1081 gtctcgtcgg ggttccgggc ctcgatgccg gtcggcgcga ggttggaaac gccgaggggt
     1141 ggcagggaca gcgtctcggt gtgggtcccg cccacggtga ccgtgcacgt cacccggtcg
     1201 ggtccctcgg cccggaagtc acgggcccga taggaccagt tgggcaaccc ggcgctgagc
     1261 gcctgcatga ggccgatgaa accgtcggcg tcgatcggct ccgggaccgg gccggaaaag
     1321 ctgaagtcgt cggtcatcat cgcccgggct tggtcgaagc gttgcgcctc aagcaggtcc
     1381 acgaccgtct tcgcgacgtc gaggttcccc atggtcgcct ccttcgaggg cagtggtcgg
     1441 tacgaccagg accgtacgcc aggctcggac gattcggatc accgaggcca ggtagtgctc
     1501 actgtcagcg ggctcaaaga ccagcctgcc ttctgcaact ggtgcgcagc cattaggttc
     1561 ggcgggtcgg agggggaccc atggcgctgg agctggacct gcgcgcggtg agcgccgaac
     1621 gggcccgccg ttacgtcgcc gacgggtcgt ggaccgacga gaccctcggg acgatcctcg
     1681 gcgccggcct ggtcgagggc cgagacctga cgctcgcgat ccactccgaa acccgcccgt
     1741 ggcggggcac gttcgccgac gggtacgacc tggcctgccg agtcgccggc gggctgcgcg
     1801 ccgtcggggt ccggcccggt gaccccgtcg cgttccagct ccccaactgg gtcgaggccg
     1861 ccgcgacgtt ctgggccatc gcgctgctcg gggcggtccc cgtccccatc gtccacttct
     1921 acgggcccaa ggaggtggcg ttcatcctcg agcagtcccg agcccaggtg ctcgtgaccg
     1981 ccgaccagtt cggccgcctc gactacctgg ccaacctcga cacgttccgg cctcgcctcg
     2041 acgggctgcg ggaggtgttc gtggtgggcg agcgcatagc ggctggcacc cggccgttcg
     2101 aggatctcgc cggcgctgac ccggtcgacg ggcccgtcgc caccgatccg tcggcgccgg
     2161 ccctgatcgc gtacacgtcc ggcacgaccg cggacccgaa gggggtcgtg cactcgca
//
LOCUS       DATCCH010000163         2512 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_216252, whole genome shotgun sequence.
ACCESSION   DATCCH010000163 DATCCH010000000
VERSION     DATCCH010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216252"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VIJ44_03300"
     CDS             complement(<1..202)
                     /locus_tag="VIJ44_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007502744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HEY5164959.1"
                     /translation="MGVRVGGGQGFYGDTPRSVEGLLEAGVDYLCLEALAELTLAILQ
                     KDRQKDETRGYTKDLPAYLRAAL"
     gene            complement(206..1669)
                     /locus_tag="VIJ44_03305"
     CDS             complement(206..1669)
                     /locus_tag="VIJ44_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY5164960.1"
                     /translation="MTDDLAFLDAVSQAELVRRGDLSPLELVDAAITRIEKLNPELNA
                     VIHPRFERARGEAAGSLPDGPFRGVPIVIKDLDGSSAGDPLHNGNQALKAAGYVAEHD
                     TWLVSRLRAAGFVIVGKTNTPEFGLMPTTEPLAYGPTRNPWNTDHSTGGSSGGSAAAV
                     ASGMVAVGHAGDGGGSIRIPASECGLFGLKPTRGRVSLGPDLGEAWAGFVVRHAVTRS
                     VRDSAAVLDVIAGPMPGDPYYAPPPSRPFATEPGTDPGRLRIGVRTTAPGAMAATDDE
                     CVDAARDAAELLTSLGHHVEDAAPAALDDEALMAHFTTVLTTSVVHEVQGAGRAIGRD
                     LTVDDVEPLTWQYYEAGLANQAVQYLEAINALHHWARDVASWWTPSDEGGAGFDLLLT
                     PSLAEPPPRIGDVVGTREDPWHGMARASVFACYTAPFNVTGQPAMSVPLQWEASENLP
                     IGTQLVAASAREDLLLRVAAQLETARPWAERRPAVHG"
     gene            complement(1689..>2512)
                     /locus_tag="VIJ44_03310"
     CDS             complement(1689..>2512)
                     /locus_tag="VIJ44_03310"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018334568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEY5164961.1"
                     /translation="MIGMAGAGLGEDLLLANELVDEDRLKALATLDARVTVAVDSPRT
                     VTAAADAGLTECLIDVNVGLPRCGCTPDDAAALAELAVSRGLSVRGVMGYEGHVVGLT
                     DQSERVRQTAECMALLRQAHDAVGGDLISAGGTGTYDVNTVATEIQAGSYALMDTAYG
                     ALGLPFRRALAITATVVSTNSSGGYAVADCGLKSLGMDHGNPTIDDATVWFCSDEHVT
                     FSINDDGADARLPAVGDRISVWPAHVDPTVAYHERMHITNGDQVIETWDVDLRGW"
BASE COUNT          352 a          876 c          907 g          377 t
ORIGIN      
        1 gcaacgcggc gcgcaggtag gcgggcaggt ccttggtgta gccgcgggtc tcgtccttct
       61 ggcggtcctt ctggaggatc gcgagcgtca gctcggccaa cgcctcgagg cagaggtagt
      121 cgaccccggc ttcgaggagg ccttcgacgg accggggcgt gtcgccgtag aaaccctggc
      181 cgccgccgac gcggacaccc accgttcagc cgtggacggc cggacggcgc tcggcccagg
      241 gccgcgccgt ctcgagctgc gcggccacgc gcagcagcag gtcctcacgg gcggacgccg
      301 cgacgagctg ggtcccgatg gggaggttct cgctcgcttc ccactgaagc ggcacggaca
      361 tcgcgggctg accggtcacg ttgaacggcg cggtgtaaca cgcgaacacc gaagcccgcg
      421 ccatgccgtg ccacgggtcc tcacgggtgc cgaccacgtc gccgatccgc ggtggcggct
      481 cggccaggct cggtgtcagc agcaggtcga acccggcccc gccttcgtcg ctcggcgtcc
      541 accacgacgc gacgtcccga gcccagtggt ggagcgcgtt gatcgcctcg aggtactgca
      601 cggcctggtt cgcgaggccc gcttcgtagt actgccaggt gaggggttcg acatcgtcga
      661 cggtcaggtc ccgcccgatg gcccgtccgg ctccctggac ttcatggacg acgctggtgg
      721 tgaggaccgt ggtgaagtgc gccatcaggg cctcgtcgtc gagtgccgcg ggtgccgcgt
      781 cctcgacgtg atggccgagg gacgtcagca gctccgcggc gtcacgggcc gcgtcgacgc
      841 actcgtcgtc ggtggcggcc atcgctcctg gcgcagtggt ccgcaccccg attcgcagcc
      901 ggcccgggtc cgttcccggc tccgtcgcga acggtcgtga cggtggtggc gcgtagtacg
      961 ggtcgcccgg catcggcccg gcgatgacat cgaggaccgc tgcgctgtca cgcaccgatc
     1021 gcgtcaccgc gtggcggacc acgaaaccgg cccaggcctc accgagatcg gggccgagcg
     1081 acacccgtcc gcgcgtcggc ttcagcccga acagcccgca ctcactggcc gggatccgga
     1141 tcgatccgcc gccgtcgccg gcgtggccca ctgcgaccat cccggaggcg accgcggcgg
     1201 cggatccgcc gctcgagccg ccggtgctgt ggtcggtgtt ccacgggttc cgggtcggcc
     1261 cgtacgccag cggctccgtg gttggcatca gcccgaactc gggcgtgttc gtcttgccga
     1321 cgattacgaa ccccgcggcg cgcagccgtg acacgagcca ggtgtcgtgc tcagcgacgt
     1381 agccggcggc cttcaaggcc tggttgccgt tatggagcgg atcgccggcg gaggagccgt
     1441 cgaggtcctt gatgacgatc gggacacctc ggaacgggcc gtccggcagc gaaccggcgg
     1501 cctcgccgcg cgctcgctcg aagcggggat gaatcacggc gttcagctcg ggattcagct
     1561 tctcgattcg cgtgatcgcc gcgtcgacga gctcgagggg cgaaaggtcg cctcgccgga
     1621 cgagctcggc ctgcgacacc gcgtccagga aggcgaggtc gtcggtcatg ggctgcgacg
     1681 gtagccgctc accagccgcg caggtcgacg tcccaggtct cgatcacctg gtcgccgttg
     1741 gtgatgtgca tccgctcgtg gtaggcgacg gtcgggtcaa cgtgcgcagg ccagaccgag
     1801 atgcggtcac cgacggcggg gaggcgcgca tcggcaccgt cgtcgttgat cgagaatgtc
     1861 acgtgctcgt cggagcagaa ccacaccgtg gcgtcgtcga tggtggggtt gccgtggtcc
     1921 ataccgagcg acttgagccc gcagtcggcg acggcatacc cgccggagga gttcgtggac
     1981 accaccgtcg cggtgatcgc caaggcgcgc cggaacggga gacccaaggc gccgtacgcg
     2041 gtgtccatga gcgcgtacga gccggcttgg atctccgtgg cgacggtgtt cacgtcgtag
     2101 gtgccggtcc caccggcgga gatcaggtca ccaccgaccg cgtcgtgggc ctggcggagg
     2161 agggccatgc actcggcggt ttggcggacc cgttcactct gatcggtgag cccgaccacg
     2221 tggccttcgt accccatgac gccacgcacc gagagtccgc gcgagacggc cagctcggcg
     2281 agggccgcgg cgtcgtcggg cgtgcacccg cagcggggga ggccgacgtt cacgtcgatc
     2341 aggcattcgg tcaggccggc gtccgcggcc gcggtgaccg tgcgcggtga atccaccgcc
     2401 acggtgacgc gcgcgtcgag cgtcgccagc gccttcagcc ggtcctcgtc caccagctcg
     2461 ttcgcgagca gcagatcctc cccgagcccg gccccggcca tcccgatcat ct
//
LOCUS       DATCCH010000164         3231 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694 k141_9014,
            whole genome shotgun sequence.
ACCESSION   DATCCH010000164 DATCCH010000000
VERSION     DATCCH010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9014"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="VIJ44_03315"
     CDS             complement(<1..845)
                     /locus_tag="VIJ44_03315"
                     /inference="COORDINATES: protein motif:HMM:NF037131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HEY5164962.1"
                     /translation="MSDQVLTDQDRELLNAVQWDFPLEPRPFAALGDRLGLSEPEVRA
                     RVAAVKDTGVLRQLSAIFDTRALGYNSSLVAAQIDPDRVDEAAATISAHPGVSHNYKR
                     NHSFNLWYTIAVPPGESLDEHLEVLHQQSGAIVTRKLPTLKLYKIGVKLDMTGQTAAD
                     AKVAVADHERPERREHMDAPVLSDLEIAAIRVVQEDLPLVERPFAAQAAELGCSEDDV
                     LGLLASFKDRKLMRRFAAVMNHRTAGFKANAMGVWAVPDDRLDEIGPAMAGFALVSHC
                     YRRPT"
     gene            complement(886..2046)
                     /locus_tag="VIJ44_03320"
     CDS             complement(886..2046)
                     /locus_tag="VIJ44_03320"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY5164963.1"
                     /translation="MPVEEPEDEHEAGLPPHPLDRVWFHPSELGSAMAAWQDTPATKH
                     RDWGLAALVGLLSVAATVGILAAVGLFGGGSHSTSRESVASVLPGSATLRDSAANVVA
                     AASPSVVSVRVSGIGGEVHGSGVAIDGSRVLTSAALVGAGTTFMVAAADGKVETARVA
                     GTDPQTDLTLLEVGDSDLPAAHLGRSDSLLVGDGVVALGLSVEDHHWAGGGIVSALHR
                     IEPTPSGGMLTSVIETDVKLAPTAEGGALLDSGGSVVGILSASLPGQAIPIDLAQTVV
                     EEIESSGRVHHAWLGVGTVDATDRGGGGARVTVVVPGSPAAKTGLAVGDVITGVSGGR
                     VADTGDLVAAVQELRPGDPIMLTAWRGNQRGVHQLALEDGTGPAGTAYGVMG"
     gene            2145..2624
                     /locus_tag="VIJ44_03325"
     CDS             2145..2624
                     /locus_tag="VIJ44_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEY5164964.1"
                     /translation="MLAPNNTSDWIALTSHALPVDGATTWAATAASGAVVTFCGIVRD
                     RSEGRPGVTGLSYEAYEEEANRRLAAVAAETRRRWPVVERLVLLHRTGDLGLSEPSVV
                     VVASSPHRPEAFEAARFAIDTLKETVPIWKREHWAGGSDWAECGHPVRDVREPHPAA"
     gene            2634..>3231
                     /locus_tag="VIJ44_03330"
     CDS             2634..>3231
                     /locus_tag="VIJ44_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEY5164965.1"
                     /translation="MARDLAIDLGTANTLVYARGQGIILNEPTVIALNSRTQDVLAMG
                     HEAWHMIGRTPGHIVAVRPLRGGAITDFEITQRLIRLIFARAGITRLQRARVLVCVPS
                     AITHVEQRAVLEATRRAGAAQTYLIEQPMAAAIGASLPIHEPLGNMVIDIGGGTSEMA
                     VISMGGIVALQAVRIGGFDIDAAISNHLRRAHGVAVGER"
BASE COUNT          472 a         1180 c         1117 g          462 t
ORIGIN      
        1 taggtcggcc gccggtagca gtggctcacc agcgcgaagc cggccatcgc gggaccgatc
       61 tcgtcgaggc ggtcgtcggg gaccgcccag acccccatcg cgttggcctt gaaccccgcg
      121 gtgcggtggt tcatcaccgc cgcgaagcgc cgcatcagct tgcgatcctt gaacgacgcg
      181 agcaggccga ggacgtcgtc ctccgagcag ccgagctcgg cggcctgcgc ggcgaagggg
      241 cgctccacga ggggaaggtc ctcttgcacg acgcggatgg ctgcgatctc caggtcggag
      301 agcaccgggg cgtccatgtg ctcgcgacgc tccgggcgct catggtctgc caccgcgacc
      361 ttggcgtcgg ccgcggtctg accggtcatg tcgagcttca ccccgatctt gtacagcttc
      421 agggtgggga gcttgcgggt gacgatggca ccggactgct ggtggagcac ctcgaggtgc
      481 tcgtcgaggg actccccagg cgggacggcg atcgtgtacc agaggttgaa tgagtggttg
      541 cgcttgtagt tgtggctcac acccggatgg gcgctgatcg tcgcggccgc ctcgtcgacc
      601 cggtcggggt cgatctgagc cgcgacgagg gaggagttgt agccgagggc ccgggtgtcg
      661 aagatggcgg agagttggcg gaggaccccg gtgtccttca ccgcggcgac acgcgctcgc
      721 acctcgggct cggagagccc cagccggtcg cccaatgcgg cgaacgggcg aggctcgagc
      781 gggaagtccc actggaccgc gttcaggagc tcacggtcct gatcggtcaa cacctggtcg
      841 ctcatctcga tcccatcgtg gcggccggtg gccgcaccgg caaattcagc ccatcacgcc
      901 gtaggccgtc cccgctggtc ccgtgccgtc ctccaacgcg agctgatgca caccccgctg
      961 gttgcctcgc cacgcggtga gcatgatcgg gtcccccggg cggagttctt ggaccgcggc
     1021 gaccagatcg ccggtgtcgg cgacgcgccc gccgctcacg ccggtgatga catcgccgac
     1081 cgccaggccg gtcttcgccg ccgggctgcc cggcacgacg accgttaccc gcgcgccacc
     1141 gccgccgcgg tcggtcgcgt cgacggtccc gacgccgagc cacgcatggt gcacccggcc
     1201 gctggactcg atctcctcca cgacggtctg ggccaagtcg atcgggatcg cttgaccggg
     1261 cagcgaagcc gagaggatcc cgaccaccga accaccactg tcgagcaggg caccaccttc
     1321 ggcggtcggg gcgagcttca cgtcggtctc gatcacgctg gtcagcatcc cacccgacgg
     1381 tgtcggctcg atgcggtgca gggcgctcac gatcccgccg ccggcccagt ggtgatcctc
     1441 gacgctcaac cccaacgcga ccacaccgtc accgaccagc agcgaatcgc tgcggccgag
     1501 atgggccgcg gggagatcgc tgtcacccac ctcgaggagc gtcaggtcgg tctggggatc
     1561 ggtccccgcg acgcgggcgg tctcgacctt tccgtcagcg gctgcgacca tgaacgtcgt
     1621 gcccgcgccg accagcgcgg cgctggtcaa gacccgagac ccgtcgatcg cgacaccgga
     1681 gccgtggacc tcgccgccga tgccgctgac ccgtacggac accacactcg ggctcgccgc
     1741 cgcgaccacg ttcgcggccg agtcgcgcag cgtcgccgag ccgggcagga cgctcgccac
     1801 cgattcgcgg ctggtggagt gacttccgcc cccgaacagg ccgaccgcgg cgaggatccc
     1861 cacggttgcc gccacgctca gcagcccgac gagcgcggcg agtccccagt ctcggtgctt
     1921 ggtggccggc gtgtcctgcc acgccgccat cgccgagccc agttcgctcg ggtggaacca
     1981 gacccggtcg aggggatgcg gtgggagccc ggcctcgtgc tcgtcttcgg gctcctcgac
     2041 gggcatggat tcaactgtac cgacggggtc tcgcgttcga accgcgggcg aggccagacg
     2101 cggccctggg ggccgcgccg cgccgcttca ctaccattgc cgacgtgctg gccccgaaca
     2161 acacgtcgga ctggatcgcg ctgacatccc acgcgctccc ggtcgacggg gccacgacgt
     2221 gggcggcgac ggccgcgagc ggagccgtcg tcacgttctg cggaatcgtc cgtgatcgct
     2281 ccgagggacg cccgggagtg accggcctct cctacgaggc gtacgaggaa gaagcgaacc
     2341 gccgcctcgc cgcggtcgcg gccgagaccc gccggcgctg gccggtcgtc gaacggctgg
     2401 tgctgctcca ccgcaccggc gacctgggcc tctccgagcc gtcggtggtt gtggtggcgt
     2461 ccagccccca ccgaccggag gcgttcgaag ccgcccggtt cgccatcgac accctgaagg
     2521 agaccgtccc gatctggaag cgcgagcact gggctggcgg ctcggactgg gcggagtgcg
     2581 gccacccggt ccgcgacgtc cgcgagcctc accccgcggc gtagccgagc cccatggccc
     2641 gcgacctcgc catcgacctc ggaaccgcga acacgctcgt ctacgcgcgc ggacagggga
     2701 tcatcctcaa cgagccgacg gtcatcgcgc tcaacagccg cacccaagac gtcttggcca
     2761 tgggccacga ggcgtggcac atgatcggcc ggactccggg tcacatcgtc gcggtgcgcc
     2821 ccctccgcgg cggcgcgatc accgacttcg agatcaccca gcgcttgatc cgcctgatct
     2881 tcgcccgggc cggcatcacc cggctgcagc gggcccgggt cctggtgtgc gtgccgtcgg
     2941 cgatcaccca cgtcgagcaa cgggcggtgc tcgaagccac ccggcgggcc ggggccgcgc
     3001 agacctacct gatcgagcag ccgatggccg ccgcgatcgg cgcgtcgctc cccatccacg
     3061 agccgctcgg caacatggtc atcgacatcg gcggtgggac cagcgagatg gccgtcatct
     3121 ccatgggcgg gatcgtcgcc ctccaagccg tccgcatcgg cgggttcgac atcgatgcgg
     3181 ccatctcgaa ccatctccga cgtgcccacg gcgtcgcggt gggggagcgc a
//
LOCUS       DATCCH010000165         4028 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_516544, whole genome shotgun sequence.
ACCESSION   DATCCH010000165 DATCCH010000000
VERSION     DATCCH010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4028
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516544"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1985)
                     /locus_tag="VIJ44_03335"
     CDS             complement(<1..1985)
                     /locus_tag="VIJ44_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEY5164966.1"
                     /translation="MTASAPIDAGAPGPAAPEHETPPAGLSAAEVADRVTRGLTNGGG
                     ERTSRSYSEIVRANLFTRFNAILGTMLAVILVVGQPQDGLFGIVLVANALIGITQEIR
                     AKRKLDSLAVLSAPIARVVRDGALHEIPVEAIVLDDLVELRTGDQVPADGVVRSTAGL
                     EVDESLLTGESDSIDKEPGAPVMSGSIVVAGTGRFQATAVGSASYARKLALEARQFRL
                     VRSELVDGINFLLRLIQYALFPIAALLLWQQLRTDNVSSALTSTVAGVVGMVPEGLVL
                     LTSLAFGIAAVTLARRNVLVQELPAVEGLARVDIVCLDKTGTLTDGNVVFHRYEVLGS
                     PADDIEAALGALADDPNRNATLAAVGSAFEPPPGWTRTAATPFSSARKWSAATFESQG
                     TWILGAPEMVLPEAGADDPVRARADELAASGKRVLILAASAAPAGEQLPEPLTAVALV
                     LLEEQIRPDAKETLAYFAEQGVALRVISGDNPRTVAAVAARVGLPGADAPYDARELPD
                     DEDELGEVLETHTVFGRVTPQQKRAMVHALQARGHVVAMTGDGVNDALALKDADIGVA
                     MGSGAAATRAVAQLVLLDGRFATLPGVVAEGRRVIANIERSANLFVTKTVYAIVLAVS
                     VVILDWKYPFLPRHLTVISTFTIGIPGFFLALAPNTRR"
     gene            2040..>4028
                     /locus_tag="VIJ44_03340"
     CDS             2040..>4028
                     /locus_tag="VIJ44_03340"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5164967.1"
                     /translation="MSGPYPTRWVSDVVLSDGGTVHVRPIRNSDSEALLGLYSRLSDE
                     SLYLRFFSPVPAPTARQLEALTDLDYNGRFALVAELGDDIVAVARFDRLDEQTDAEVA
                     FTVQDDQQGRGLGSILLEHLAAAARELGVRRFVASTLSGNQKMLHVFRDAGFVVTRHL
                     DGGTVEVSFPINPTPASIDAQNVREHIAEARSMQRLLKPASVAVIGASRQPGTIGHAV
                     LRNILNGDFTGPVYPINPAARSVAGVRAYASVRDVPDEIDLGVIAVPAAHVLEVARDC
                     AAKQVAGMVVISAGFAEVGGGHASAERAIVELARHNGMRIIGPNCMGIINTHPDVRLN
                     ATFAPFTPAPGRVGFASQSGGLGIELLARAGEYGLNVSTFVSMGNKADVSSNDLIQYW
                     DEDPDTDVILLYLESFGNPRKFSRLARRVARRKPIVAVKGGRTPAGERATSSHTAALA
                     SPDVAVDALFRQAGVIRVDTLEELFGVGTVVRHQPLPPGRRVGIITNGGGPGILAADA
                     CTAAGLEVPELSAATQAVLRAFVSADAGLSNPIDLVASATAEQYERAVETLLTDDAID
                     SLLVLFVPPLVTRAEDVARAVTAGSAEAGDKPIVACFLGRNGTIDLLSVGEDGRQIPS
                     FAFPEAAAGALAHAAQLAEWRDRPEGHVPELDHLDLDAA"
BASE COUNT          609 a         1349 c         1451 g          619 t
ORIGIN      
        1 cgccgggtgt tgggcgcgag cgccaggaag aacccgggga tgccgatcgt gaacgtgctg
       61 atgaccgtca ggtggcgggg aagaaacggg tatttccagt cgaggatgac caccgagacg
      121 gccaacacga tggcgtacac cgtcttggtc acgaacaagt tcgccgaccg ttcgatgttg
      181 gcgatcaccc gtcgaccctc ggcgacgacg ccgggcagcg tcgcgaaccg tccgtcgagg
      241 agcacgagtt gggcgacggc gcgcgtcgcg gccgcgccgg atcccatcgc gaccccgatg
      301 tcggcgtctt tgagagccag cgcgtcgttg acaccatcgc cggtcatggc gacgacgtgg
      361 ccgcgggctt ggagggcgtg gaccatcgcg cgcttctgct ggggtgtgac ccggccgaac
      421 acggtgtggg tctcgagcac ctcgccgagc tcatcctcgt cgtcggggag ctcgcgtgcg
      481 tcataggggg catcggcccc tgggaggccg acccgggccg cgaccgcggc gacagtgcgc
      541 gggttgtcac cggagatcac tcggagcgcg accccttgct cggcgaagta ggcgagggtc
      601 tctttggcgt cgggtcggat ctgctcttcg aggaggacga gggcgaccgc ggtgagcggc
      661 tcggggagtt gctcgccagc gggcgcggcg ctggcggcga ggatcaggac ccgtttgccg
      721 gacgcggcga gctcgtcggc ccgggcccgg accgggtcgt cagcgccggc ctcggggaga
      781 accatctcgg gcgcgccgag aatccaggtt ccctgggact cgaaggtcgc ggcgctccac
      841 ttgcgggcgg acgagaacgg ggtcgccgcg gtgcgggtcc agcccggtgg cggttcaaac
      901 gcggatccca ccgcggcgag cgtggcgttg cggttcggat cgtcggcgag cgcaccgagc
      961 gccgcttcga tgtcgtcggc gggtgacccg aggacttcgt agcggtggaa gaccacgttg
     1021 ccgtccgtga gcgtccccgt cttgtcgaga cagacgatgt cgacgcgcgc cagtccctcg
     1081 accgccggga gctcctggac caagacgttg cggcgagcga gcgtgaccgc ggcgatcccg
     1141 aacgccaggc tggtcagcag cacgaggccc tcgggaacca tgcccacaac accggcgacg
     1201 gtgctggtca gcgcggacga gacgttgtcg gtgcggagct gctgccagag gagcaacgcc
     1261 gcgatgggga agagggcgta ctggatgagg cggagcaaga agttgatgcc gtcgacgagc
     1321 tcggagcgga cgaggcgaaa ctggcgagct tcgagcgcca gcttgcgggc gtacgaggcg
     1381 gagccgaccg cggtcgcctg gaagcggccg gtgccggcca cgacaatgct tcccgacatg
     1441 accggcgcgc cgggctcctt gtcgatcgag tccgactcac ccgtcagcag cgattcgtcg
     1501 acctcgaggc ccgcggtgct tcggaccaca ccatcggccg gaacttggtc gccggttcgc
     1561 agctcgacga ggtcgtcgag cacgatcgcc tcgacgggga tctcgtgcag cgcaccgtcg
     1621 cgtaccactc gggcgatcgg cgcgctcaac accgcgagag agtcgagttt ccgcttggcg
     1681 cggatctctt gggtgatgcc gatcagcgcg ttcgcgacga gcacgatgcc gaacagcccg
     1741 tcctgaggct gaccgacgac gaggatgacc gccagcatcg tgccgaggat ggcgttgaac
     1801 cgagtgaaca ggttggcgcg gacgatctcg ctgtaggacc ggctggtgcg ctcaccaccg
     1861 ccgttggtga gaccgcgggt gacacggtcc gcgacctcag ccgcgctcag gccggcgggg
     1921 ggcgtctcgt gctccggcgc ggccgggccc ggcgcaccag catcgatggg cgccgacgcg
     1981 gtcatcgccg gcgacggtag ccgaccgcac ccgcgggttt gatcgatacg ctcccgttca
     2041 tgagcggtcc ttaccccacc cgctgggtgt cggatgtcgt gctcagcgac ggtgggacgg
     2101 tgcacgtccg gccaatccgc aacagcgact ccgaagcgct gctcggcctg tattcgcggc
     2161 tctccgacga atcgctgtac ctccggttct tctcgccggt accggcaccg accgcgcgtc
     2221 agctcgaggc cctcaccgac cttgactaca acggtcggtt cgcgctcgtc gccgagctcg
     2281 gcgacgacat cgtggccgtg gctcgcttcg atcgcctcga cgagcagacc gacgccgaag
     2341 tcgcgttcac ggtgcaggac gaccagcagg gtcgcgggtt gggttcgatc ctcttggagc
     2401 atctcgccgc cgcggcgcgc gagctcgggg tgcggcggtt cgtcgcgtcg acgctgtccg
     2461 ggaaccagaa gatgctgcac gtcttccggg acgcgggatt cgtggtaacc cgacacctcg
     2521 acggtggcac cgtggaggtg tcgttcccga tcaatccgac cccggcctcg atcgacgccc
     2581 agaacgtccg ggagcacatc gccgaggctc gatcgatgca gcggctgctc aagccggcgt
     2641 cggtcgccgt gatcggggcg agccgccagc ccggcaccat cggccatgcg gtccttcgga
     2701 acattctcaa cggggacttc accggcccgg tgtatccgat caaccctgca gcccgctccg
     2761 tcgccggggt ccgggcttac gcgtcggtcc gcgacgtccc cgacgagatc gacctggggg
     2821 tgatcgcggt cccggcggcg cacgtgctcg aggtcgcccg tgactgcgcc gcgaagcagg
     2881 ttgccggaat ggtcgtgatc tcggccgggt tcgccgaggt cggcgggggg cacgcgtcag
     2941 cggagcgagc gatcgtcgag ctcgcccgac acaacgggat gcgaatcatc gggcccaact
     3001 gcatgggcat catcaacacc catcccgatg tgcgcctcaa cgccacgttc gcgccgttca
     3061 cacccgcgcc ggggcgggtc ggattcgcgt cgcagtccgg tgggctcgga atcgagctgc
     3121 tggcccgggc gggtgagtac ggcctgaacg tctcgacctt cgtgtcgatg ggcaacaagg
     3181 ccgacgtgag cagcaacgat ctcatccagt actgggacga ggatcccgac accgacgtca
     3241 tcctcttgta cctggagtcg ttcgggaacc ctcgcaagtt ctcccggctg gcgcgtcgcg
     3301 tggctcggcg caagccgatc gtggcggtca aggggggacg cacccctgcc ggcgagcgcg
     3361 cgacgtcgtc gcacactgca gcgctggcct cgcccgatgt ggccgtggac gcgctgttcc
     3421 gtcaggccgg cgtcatccgg gtcgacaccc tcgaagagct cttcggcgtc ggaacggttg
     3481 tccggcatca accactccca ccggggcgtc gggtcggcat catcacgaac gggggtgggc
     3541 ccgggatcct tgccgcggac gcatgcaccg cggccggtct cgaggtccca gagctctcag
     3601 cggcgacgca ggcagtgctg cgggcgttcg tgtcggccga cgccggcctc agcaacccga
     3661 ttgatctcgt cgcgtcggcc accgccgagc agtacgagcg tgccgtggag accctgctga
     3721 ctgacgatgc gatcgacagc ctgctggtgc tgttcgtgcc gccgctcgtc acccgagccg
     3781 aggacgtcgc tcgggccgtc acagcgggca gcgccgaggc gggtgacaag ccgatcgtgg
     3841 cgtgcttcct cggccggaac gggacgatcg acctcctgtc cgtcggtgag gacggacgcc
     3901 agatcccgag cttcgccttc cccgaagcgg cagcgggggc gttggcgcac gcggcgcagc
     3961 tcgccgagtg gcgcgatcgg cccgagggac acgtccccga gctcgatcat ctcgacctcg
     4021 acgcggcg
//
LOCUS       DATCCH010000166         2547 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_101904, whole genome shotgun sequence.
ACCESSION   DATCCH010000166 DATCCH010000000
VERSION     DATCCH010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101904"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="VIJ44_03345"
     CDS             <1..612
                     /locus_tag="VIJ44_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008356123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY5164968.1"
                     /translation="LRIQVDREHENSWSDVESDIFSEPFLLTRPLLEAIRATEPVVLL
                     IDEVDRVEIETEALLLEVLSDFQVSIPELGTIVGTQRPLVILTSNNTRELSEALKRRC
                     LYLHIDYPDLEREKDIVRVRVPEIDDELADQVARVVRSIRELELKKAPSISETIDWAR
                     TLLFLGTSEISPEIIGDTLHVLLKYQSDIAKARKELVPAGDAT"
     gene            617..2032
                     /locus_tag="VIJ44_03350"
     CDS             617..2032
                     /locus_tag="VIJ44_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY5164969.1"
                     /translation="MLNVLQGFVHELRSAGLPVSMTENLDAMRAVEHIDIADRDLFRA
                     TLGATLVKHHRHWPAFETVFDVYFSLYSPGVDADDAHAEEWAEMVSEASGGGGGGGEL
                     TREELAQMLLDALMNMDRDQLRRLAATAVTQFAGMEPGRPVGGTYYLYRTLRQLDLDD
                     LTARLIGRAQEQGDVPADDLAARLQAEEYQARLRMLRELVEAEIRRRLVADRGVEAMA
                     RTLRKPLPEDVDFMHASREEMLALQRAIYPLTRALAARLAQRRRRRHRGHLDFRKTVR
                     ASLSYGGVPAEPKFRHPHPSKPEIMVVADISGSVASFARFTLQFVYAMASQFSKVRSW
                     VFIDGIDEVTRFFQGSDDVTDAVHRVNTEADVVWVDGHSDYGHAFEVFHNRHLSEVTN
                     KTSLIFLGDARNNYHASQAWVLRALGDRARHLSWLNPEPRGYWDTGDSIMAEYVPYCD
                     GVYECRNLRQLERFVSTVAEG"
     gene            complement(2119..>2547)
                     /locus_tag="VIJ44_03355"
     CDS             complement(2119..>2547)
                     /locus_tag="VIJ44_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164970.1"
                     /translation="GVSVEEVRIVRTFPDPRLDALESAADLCEATSVVELHQTFVAQT
                     CREFLADWSVMLENRTVLARVGAPPPDEMLRALAAGTAASPLVADGTTGPDDLAVAVL
                     EMHHSTLLVGRDGHPFRRRERAQLRALAHIADRAWTLLDR"
BASE COUNT          420 a          946 c          788 g          393 t
ORIGIN      
        1 ctccgcatcc aagtcgaccg tgagcacgag aactcctggt ccgacgtcga atccgacatc
       61 ttctccgagc cgttcctgct cacccggccg ctcctcgagg cgatccgcgc caccgaaccg
      121 gtggtgctcc tcatcgacga ggtcgaccgg gtcgagatcg agaccgaggc gctgctcctc
      181 gaggtcctct ccgacttcca ggtctccatc ccggagctcg gcaccatcgt cggcacccaa
      241 cggccgctgg tcatcctcac ctccaacaac acccgcgagc tgtccgaggc tctgaaacgc
      301 cgatgcctct acctgcacat cgactacccg gaccttgaac gcgagaagga catcgtgcgg
      361 gtccgggtcc ctgagatcga cgacgagctc gccgaccagg tcgcccgcgt cgtccgctcg
      421 atccgtgagc tcgagctgaa gaaggccccg tcgatctccg agacgatcga ctgggctcgc
      481 accctcttgt ttctcggcac gtcggagatc tcccctgaga tcatcggcga caccctccac
      541 gtgctcctca agtaccagtc cgacatcgcc aaggcccgca aggagctggt gcccgccggc
      601 gacgccacct gagccgatgc tcaacgtcct tcagggcttc gtccacgagc tgcgcagcgc
      661 cggtctcccg gtgtcgatga ccgagaatct cgacgccatg cgcgccgtcg agcacatcga
      721 catcgccgac cgggacctgt tccgagccac gctcggcgcc accctcgtca agcatcaccg
      781 gcactggccc gcgttcgaga ccgtcttcga cgtctacttc tcgctgtaca gcccgggtgt
      841 cgacgccgac gacgcccacg ccgaggagtg ggccgagatg gtcagcgaag ccagcggcgg
      901 tgggggaggc ggaggcgagc tcacccgcga ggagctcgcc cagatgctcc tcgacgcgct
      961 gatgaacatg gaccgcgacc agctgcgccg cctcgccgcg accgcggtga cccagttcgc
     1021 ggggatggaa ccgggacgtc ccgtcggtgg cacctactac ctgtaccgga ccttgcgcca
     1081 gctcgatctg gacgacctca cggcgcggct cattggccga gcccaagaac aaggcgatgt
     1141 ccccgccgac gacctcgcgg cgcgcctcca agccgaggag taccaggctc gactccggat
     1201 gctgcgcgag ctcgtcgaag ccgagatccg acgtcgtctt gtcgccgacc ggggcgtcga
     1261 ggcgatggcc cgcaccttgc gcaaaccgct ccccgaagac gtcgacttca tgcacgcctc
     1321 gcgcgaggag atgctcgcgc tccagcgggc catctatccg ttgacccggg ccttggccgc
     1381 gcggctggcg caacggcgcc gacgccgcca ccgaggccac ctcgacttcc ggaagaccgt
     1441 ccgggcgtcc ctgtcctacg gcggcgtccc tgcggagccg aagttccgtc atcctcaccc
     1501 gtcgaaaccc gagatcatgg tcgtcgccga catctccggc tcggtggcga gcttcgcccg
     1561 cttcacgctg cagttcgtgt acgcgatggc cagccagttc tcgaaggtcc gctcctgggt
     1621 gttcatcgac ggcatcgacg aagtcacccg gttcttccag ggctccgacg acgtcacgga
     1681 cgcggtccac cgcgtcaaca ccgaagccga cgtggtgtgg gtcgacggtc actccgacta
     1741 cggtcacgcc ttcgaggtgt ttcacaatcg ccacctgagc gaagtgacca acaagacgtc
     1801 gttgatcttt ctcggcgacg cccgcaacaa ctaccacgcg tcacaggcct gggtgctgcg
     1861 cgcccttggg gatcgggccc ggcacctgtc atggctcaac cccgagccgc gcggctactg
     1921 ggacaccggc gactcgatca tggccgagta cgtcccgtac tgcgacgggg tttacgagtg
     1981 tcgcaacttg cgccagctcg aacgcttcgt ctccaccgtc gccgaaggct gagcccgcgc
     2041 gagcacggtc cgtcgggcct ggtgcgcgcc ctcgccgggc cgcacccgac ccgaccccga
     2101 gcatcaggtc gtggggcgtc agcggtcgag caacgtccag gcccgatccg cgatgtgcgc
     2161 caacgcccgc agctgggccc gctcgcgacg ccggaacgga tgtccgtccc gaccgaccag
     2221 caacgtcgag tggtgcatct cgaggaccgc caccgccagg tcatcaggcc cggtcgtgcc
     2281 gtccgcgacc aacggcgatg ccgccgtccc cgcggccagc gcccggagca tctcatcggg
     2341 tgggggagcg cccacccgcg ccaggacggt ccgattttcg agcatcactg accagtcggc
     2401 gaggaactca cggcaggtct gggccacgaa cgtctggtgc agctccacga ccgacgttgc
     2461 ctcgcagagg tccgccgcgg actccagggc gtcgaggcgg gggtccggga acgtgcggac
     2521 gatgcgcacc tcttcgaccg agacgcc
//
LOCUS       DATCCH010000167         2651 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_475325, whole genome shotgun sequence.
ACCESSION   DATCCH010000167 DATCCH010000000
VERSION     DATCCH010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475325"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="VIJ44_03360"
     CDS             complement(<1..696)
                     /locus_tag="VIJ44_03360"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY5164971.1"
                     /translation="MPRCRRGSSRLTRLGPILAGFVAFGVTGAALMAVPAVGAPGRGR
                     DPAGFDRVDSAMQRRVRGHGGGATLVVHDQTTLFRRSYDGVHTSTLIPIASASKWLTA
                     ATLMTLVDQGRLALDDPVARFLPNFSGGSGGVTIRSLLSHTSGFPDSVCVGDDTTTLR
                     DCVDQTAAASALSSQPRTFHYSSVGYAVVGRIIEVVTGESFERAFEDRIAGPVGMRRT
                     RFDGTARPRSQNPD"
     gene            769..>2651
                     /locus_tag="VIJ44_03365"
     CDS             769..>2651
                     /locus_tag="VIJ44_03365"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEY5164972.1"
                     /translation="MDTSLVSPELNAEQRRAVEHGDGPLLVIAGAGTGKTKTLAARVA
                     TLIDRGVRPERILLLTFSRRAAGEMLRRAGQVTDPVAARLVWGGTFHAIGHRLLRVHG
                     AALGLAGGFSVLDQGDAAELMGLVRSEVATGLEDRRFPRKDTLLAIYSRVANTQRPLR
                     EVLDQAFPWCAADPDTIATIFGAYTDRKRTQALLDFDDLLLFWASATRQPHLAARLEA
                     LFDHVLVDEYQDTNRVQAEIVRAMTRASSGVMVVGDDAQAIYGFRAATVRNILDFPSH
                     YPGTTIVALEENYRSTPPILALANAVIAEAAEQHDKQLWSRRPTGRPPQLVTCADEQS
                     QADEVCASVLAHHEEGIALRDQVVLFRAAHHSDLLEVELTRRGIPFVKYGGLRFLEAA
                     HIKDLLALLRVLDNPYDELAWFRVLQHVDGVGPTSARRLLETMGVRPRSGDARHPVER
                     ILDAGPAVRAAARPELADLGSALAACADGERIEPAAQVARLRQFLDPVVRRRYEHPEN
                     RLHDLEELEHLALTYANRAQLVAELVLDPPTATSDLAGPPTLDEDYLVLSTVHSAKGG
                     EWDAVHVIHAADGMFPSDMATRDPDEIDEERRLFYVALTRARDHLHVYFPLRYFHRRV
                     GHG"
BASE COUNT          418 a          928 c          894 g          411 t
ORIGIN      
        1 gtcgggattc tgggaccggg gtcgcgccgt gccgtcgaat cgagttcgtc gcatcccgac
       61 cggacccgcg atccggtctt cgaaggctcg ttcgaatgat tcaccggtga ccacctcgat
      121 gatccttccc accaccgcgt acccaaccga ggaatagtgg aacgtacggg gttggctcga
      181 caacgcggac gcggcggcgg tctggtccac gcagtcgcga agggtggtgg tgtcatcgcc
      241 gacgcacacg gaatcgggaa aacccgacgt gtgcgacagc agcgagcgga tcgtcacccc
      301 gccgcttccg ccgctgaaat tgggaaggaa gcgggccacg ggatcgtcga gggccagtcg
      361 tccctgatcg acaagggtca tgagcgtcgc cgcggtcagc cacttgctgg cggacgcgat
      421 ggggatgagg gtgctggtgt gcacgccgtc gtaggagcgg cgaaacaagg tggtctggtc
      481 gtggacgacg agggtggccc cccctccgtg cccgcggacc cggcgctgca tcgccgagtc
      541 gacccggtca aagccagccg ggtcgcggcc tcgcccggga gcgcccaccg cgggcaccgc
      601 catcagggcc gcgcccgtca cgccgaacgc cacgaagccc gcgaggatcg ggcccaggcg
      661 ggtcagccgg gaggagcctc gacgacaccg tggcatcgcc tgatcatggc gcgggcccgt
      721 ccctcgcggg tcgaacgcat gttcgtatcg gcgtctacgc tgggccgggt ggacacctcg
      781 ctggtctcgc cggaactcaa cgcggagcag cgacgggcgg tcgagcacgg tgatggtccg
      841 ctcctggtga tcgccggcgc cgggaccggc aaaaccaaga ccctggccgc tcgggtcgcg
      901 accctgattg atcggggtgt gcgaccagag cgcatccttc tcttgacgtt cagccgacgg
      961 gccgctggcg agatgctgcg ccgcgcgggg caggtcaccg atccggtcgc ggcgcgcctg
     1021 gtctggggcg ggacctttca cgccatcggt catcgcctgc tccgcgtgca cggggcggcg
     1081 ctcgggctgg ctggcggctt cagcgttctt gatcagggcg acgccgcgga gctgatgggg
     1141 ctggtgcgaa gcgaagtagc gacgggtctc gaggatcgac gcttcccacg caaggacacc
     1201 ttgctggcca tctactcgcg ggtcgccaac acccaacggc ccttgcgcga ggtgctcgat
     1261 caggcgttcc cgtggtgcgc tgccgacccg gacaccatcg cgacgatctt cggcgcgtac
     1321 accgatcgga agcggacgca ggcgttgctc gacttcgacg acctgttgct gttttgggcc
     1381 agcgcgaccc gccagcctca cctcgcggcc cggctcgagg ctctcttcga tcatgtgctg
     1441 gtcgacgagt accaggacac caatcgcgtc caggcggaga tcgttcgcgc catgacgcgg
     1501 gcctcgagcg gggtcatggt ggtcggagac gatgcccaag ccatctacgg gttccgcgcc
     1561 gcgacggttc gcaacatctt ggacttccca agccactacc cgggcaccac gatcgtggcg
     1621 ctcgaggaga actaccgctc gactccacca atcctcgccc tcgcgaacgc ggtcatcgct
     1681 gaggccgccg agcagcacga caagcaactg tggtcgcgcc ggccgaccgg acggccgcct
     1741 cagctcgtga cgtgcgccga cgagcaatcc caggccgacg aggtctgcgc ctcggtgctg
     1801 gcccatcacg aggaaggcat cgcccttcgt gatcaggtcg tcctgttccg ggctgcgcac
     1861 cacagcgatc tcctcgaggt cgagctgacc cgcaggggga tcccgttcgt gaagtacggg
     1921 ggcctccgct tcctcgaggc cgcccacatc aaggacctcc tggcgctgct gcgggtcctc
     1981 gacaacccgt atgacgagct ggcctggttc cgggtgctgc aacacgtgga cggggtgggt
     2041 ccgacctcgg cgcgccggct gctggagacg atgggggtcc gcccgcgctc cggtgacgcc
     2101 cgacacccag tggaacggat cctggacgcg ggccccgccg tccgggcagc ggctcgcccc
     2161 gagctcgccg acctgggctc ggcactcgcg gcgtgcgccg acggagaacg gatcgagccc
     2221 gcggcccaag tcgcccgcct gcgacaattc ctcgacccgg tcgtgcgtcg ccgctacgag
     2281 catcccgaga accgcctgca cgacctcgag gagctcgaac acctggccct cacgtacgcg
     2341 aaccgagctc agctcgtcgc cgaactcgtc ctcgaccccc ccaccgccac gagtgatctc
     2401 gcggggccac cgacgctcga cgaggactac ctcgtgctgt caacggtgca ctcggcgaaa
     2461 ggtggcgaat gggacgccgt gcatgtgatc catgccgcgg acgggatgtt cccatccgac
     2521 atggccaccc gcgatcccga tgagatcgac gaggagcgtc gcctcttcta tgtcgcactc
     2581 actcgcgccc gtgaccatct tcacgtctac ttcccgttgc gctacttcca ccggcgggtc
     2641 ggtcacggcg a
//
LOCUS       DATCCH010000168         4174 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_268350, whole genome shotgun sequence.
ACCESSION   DATCCH010000168 DATCCH010000000
VERSION     DATCCH010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4174
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268350"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="VIJ44_03370"
     CDS             complement(<1..751)
                     /locus_tag="VIJ44_03370"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEY5164973.1"
                     /translation="MTVVLSNRGPYRYDEDPGGELVARPGAGGLASSLGPLLLSGAAG
                     ANGAWIAAALSDGDRAAVRAGAVHAPGVQLRLLDLDAHLFRLAYEVVSNGTLWFLYHG
                     LFDLTRRPRFDRRWREAWEAYEAVNVAFTDATAELAREGDVVLVQDMHLALVPGMLRA
                     RRPDLRLTHFTHTPFCGPNSIRVLPGYAATALCESMTSVPCGFHARRWAQSYEASARE
                     MLGDGTSAPTFVAALGPDPDALTQLAASDSAR"
     gene            complement(748..1998)
                     /locus_tag="VIJ44_03375"
     CDS             complement(748..1998)
                     /locus_tag="VIJ44_03375"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015346370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY5164974.1"
                     /translation="MLERVPLLEKDLAQYADIVGDEAVAQIREAAAPLEGARVLHLSA
                     TAYGGGVAELLATHVPLLRSVGIEAEWQVLHGSDEFFAITKQVHNALQGADIEWTPQM
                     QRTYLERILDNSLLMDDGYDVVVVHDPQPVALLHFLRDHPSLANGRTRWIWRCHIDLT
                     VANPTVWEFFRPFVELHDASVWTLAEFVPASLKMDHVVEMHPCIDPLSVKNLDLARPF
                     CEELTRQYGIDTKRAIVCQISRFDPWKDPIGVVDAFRVVREQIPDAQLLLAGSMATDD
                     PEGFRVWEETERARDGDRDIHLLSNLHQVGSVQINAFQRIATVMVQKSLREGFGLTVS
                     EALWKGRPVVGGRAGGIKLQVIDDFSGYLVDSVEGCAQRVIDLLADPVGADAMGSQGR
                     EHVRANFLATKELHDWLLLLSELR"
     gene            2019..3059
                     /locus_tag="VIJ44_03380"
     CDS             2019..3059
                     /locus_tag="VIJ44_03380"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF014634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEY5164975.1"
                     /translation="MPTAVACPDKFRGTLTAPEATTAIAAGLHRAGFDDVRSVPLADG
                     GDGTLDALLAARGGSRRRVRVTGPLGDPVDAEYGLTADGLAIVEMAQASGLALVGTQN
                     DPLRASTRGTGELLAAAARTGAKRVLVGVGGSATTDGGLAALEALGWSLGGLDVTVAY
                     DVTTAFVDAATLYGPQKGATAAQVELLRRRLARLAVEYERRTGVDVTALDGAGAAGGL
                     AGGLAAIGARLEPGFEVVAAEAGLEEALDGADLVVTGEGRLDVTSFAGKVVGGVLEWA
                     GELGVAHRAVIAGQATADAREELSVVGPAQLLVLTERVWQAGEAFARAGVLVEEAAIE
                     AGRAALDPGATR"
     gene            complement(3049..3948)
                     /locus_tag="VIJ44_03385"
     CDS             complement(3049..3948)
                     /locus_tag="VIJ44_03385"
                     /EC_number="2.4.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF010496.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HEY5164976.1"
                     /translation="MGPDAHVLADRKRRLGVRVSVCLPARDEEATIGPIVATVRHELM
                     ERAGVVDEVIVLDDGSSDATAERARQAGGQVHAVGEVLPDLTVGEGKGNALWKSLYVA
                     EGDLVCFLDADLRSFEPHFVTCLLEPLLTEPGVGFVKGYYQRPLGGEPTGGGRVNELV
                     ARPLLCALFPELASVRQPLGGEYAGRRELLEVVPFVEGWGVELGLLVDIADRFGVDAI
                     AQRDLGLREHRNRSLDELAPQAMAVLLTGLRRAGVAPVDVSSALARFDRAEPDFVPVP
                     IRECPPMVSVPAYQAKFGRELSA"
     regulatory      3999..4099
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
BASE COUNT          654 a         1455 c         1489 g          576 t
ORIGIN      
        1 tccgggccga gtcggaggcc gcgagttgtg tcaacgcgtc agggtccggg ccgagggccg
       61 cgacgaacgt gggcgccgag gtcccgtctc ccagcatttc gcgggccgac gcctcatagg
      121 actgcgccca acggcgcgcg tggaacccgc aggggacgct cgtcatcgac tcacagagcg
      181 cagtggccgc atagccgggc aggacccgaa tcgagttcgg cccgcagaac ggcgtgtggg
      241 tgaagtgggt gagacgcaga tccggccggc gggcgcgcag catcccgggg acgagggcga
      301 gatgcatgtc ctggaccaga acgacatcgc cctcgcgggc caactccgcg gtcgcgtcag
      361 tgaaggccac gttcacggcc tcgtaggcct cccaggcctc gcgccaccga cgatcgaacc
      421 gaggtcgtcg ggtcaggtcg aagagcccgt ggtataggaa ccacagcgtg ccgttcgaca
      481 ccacctcgta cgccagccgg aacaggtggg cgtccaggtc caagagccgg agctgcaccc
      541 cgggggcatg cacggcgccc gccctcaccg cggcccggtc tccgtcgctc agtgccgcgg
      601 cgatccatgc gccgttggcc ccagcggccc cggacaacaa gagcggtccg agggagctgg
      661 ccagcccccc ggcgcccggg cgcgccacga gctcaccgcc ggggtcctcg tcgtagcggt
      721 acgggccccg attcgagagg acgaccgtca ccgcagctcg ctcaatagca gcaaccagtc
      781 gtggagctcc ttggtcgcga ggaagttcgc ccgcacgtgc tctcggccct gggagcccat
      841 ggcgtcggcg ccgacagggt ccgcgaggag gtcaatgacc cgctgcgcgc acccttcgac
      901 gctgtcgacg aggtagccgc tgaagtcgtc gatgacttgg agcttgatcc cgccggcgcg
      961 cccaccgacg accggccggc ccttccagag agcctcgctg accgtgagcc cgaaaccctc
     1021 acgcagcgac ttttgcacca tcaccgtcgc gatccgttgg aacgcgttga tctgcaccga
     1081 accgacctga tgcaggttcg agagcaggtg gatatcgcgg tcgccgtctc gagcccgctc
     1141 ggtctcctcc cacacccgga acccttcggg gtcgtcggtc gccatcgagc cggcgaggag
     1201 cagctgcgcg tccgggatct gctcgcgcac cacgcggaaa gcgtcgacga cgccgatcgg
     1261 gtccttccac gggtcgaacc ggctgatctg acagacgatc gcccgtttcg tgtcgatccc
     1321 gtactggcgc gtcagctcct cgcagaacgg tcgggcgagg tcgaggttct tcaccgagag
     1381 cggatcgatg cacgggtgca tctcgacgac atggtccatc ttcagtgagg ccgggacgaa
     1441 ctcggcgagc gtccacaccg acgcgtcgtg cagctcgacg aatggccgga agaactccca
     1501 gactgtcggg ttcgcgacgg tgaggtcgat gtggcagcgc cagatccacc gagtccgccc
     1561 gttggcgaga gacggatggt cgcgcaggaa atgaagcagc gccactggtt gcggatcgtg
     1621 gacgacgacg acgtcgtagc cgtcgtccat cagcagcgag ttgtccagga tccgctcgag
     1681 gtaggtgcgc tgcatttggg gcgtccactc gatgtcagcg ccctggagcg cgttgtggac
     1741 ctgcttggtg atagcgaaga actcgtcgct gccatggagg acttgccatt cggcctcgat
     1801 gccgacgctg cggagcaacg gcacgtgggt ggccagcagc tcggcgacac cgccgccgta
     1861 ggcggtggcg ctcaggtgca ggacgcgtgc gccctcgagc ggcgctgcgg cctctcggat
     1921 ctgcgcgacc gcttcgtcgc cgacaatgtc ggcgtactgg gcaagatcct tttcgagcag
     1981 cgggacacgc tcgagcatca caggccaatc tagtgagcat gcccaccgca gtcgcgtgtc
     2041 ctgacaagtt ccgcgggacg ctcaccgcgc cggaggccac gacggcgatc gccgccggcc
     2101 tgcaccgagc cggcttcgac gacgtgcgga gcgtgccgct cgccgacggc ggcgacggca
     2161 ccctcgatgc cctcctcgcg gcgcgaggtg ggagccgccg tcgggtccgc gtgaccggac
     2221 cgctcggcga tccggtcgat gccgagtacg ggctgaccgc tgacgggctg gcgatcgtcg
     2281 agatggccca ggcgagcggt ctcgccctgg tcggcaccca gaacgacccg ttgcgcgcgt
     2341 ccacgcgagg taccggcgag ctgctcgccg ccgcggcgcg caccggagcc aagcgggtcc
     2401 tcgtcggtgt gggcggcagc gccaccaccg acggtggcct tgccgcgctc gaggcgctgg
     2461 gctggtcgct cggcggcctc gacgtcaccg tcgcgtacga cgtaaccact gcctttgtcg
     2521 acgccgcgac gctctacggg ccccaaaagg gtgcgaccgc ggctcaggtc gagctgctgc
     2581 ggcggcgcct ggcgcgtctg gcggtcgagt acgagcggcg aaccggtgtc gacgtgaccg
     2641 ccctcgacgg cgcgggcgcg gccggaggtc tcgccggcgg tctcgcggcg atcggtgcgc
     2701 gcctcgagcc cgggttcgag gtcgtcgccg ccgaagccgg tctcgaggaa gccctcgatg
     2761 gcgccgacct ggtcgtgacc ggcgagggtc gcctcgacgt gaccagcttc gccgggaagg
     2821 tggtcggcgg ggtcctcgag tgggccggcg agctcggtgt cgcgcaccgg gccgtcatcg
     2881 cgggtcaggc gaccgccgac gcgcgcgagg agctgtcggt cgtcggcccg gcgcagctcc
     2941 tggtcctcac cgaacgggtc tggcaggccg gggaggcgtt cgcgcgcgcc ggggtgctgg
     3001 tggaagaagc cgcgatcgaa gcgggccgcg ctgcgctgga ccccggcgct acgcgctgag
     3061 ctcccggccg aacttcgcct gatacgccgg aacactgacc atcggcgggc actcccgaat
     3121 cgggaccggc acgaagtccg gctcggcccg atcgaagcgg gccaacgcgc tcgacacgtc
     3181 caccggcgcg acgccggccc ggcgcaaccc ggtcagcagg acggccatcg cctggggggc
     3241 cagctcgtcg agagagcggt tccggtgctc tcgcaaccca aggtcacgtt gagcgatggc
     3301 gtcgacgccg aaccggtccg cgatgtcgac gagcagaccg agctcgacgc cccacccctc
     3361 cacgaacggc accacctcga ggagctcgcg gcgaccggcg tactcgccgc cgagtggctg
     3421 gcgcaccgac gccagctcgg ggaagagggc gcacaggaga ggtcgggcga ccagctcgtt
     3481 gacgcgccca ccgccggtcg gctccccacc gagtggacgc tggtagtacc ccttgacgaa
     3541 cccgaccccg ggctcggtga ggagcggctc gagcagacag gtgacgaagt ggggctcgaa
     3601 gctgcgcagg tcggcgtcga ggaagcagac cagatcaccc tcggcgacgt acagcgactt
     3661 ccacagcgcg ttgcccttgc cttcccccac ggtgaggtcg gggaggacct cgccgacggc
     3721 gtgaacctgg ccccccgcct ggcgggcccg ctcggccgtg gcgtcgctcg acccgtcgtc
     3781 gaggacgatg acctcgtcga ccacgcccgc tcgctccatc agctcgtgac gcaccgtggc
     3841 cacgatcgga ccgatggtgg cttcctcgtc gcgggcgggg aggcacaccg agactcggac
     3901 accgagacgg cgcttacggt cggcgaggac gtgggcgtcg ggccccataa cgagtggcag
     3961 cgtacgggac ccgggggcta ctgtccccgt ccagtacgcg ctcatcgaga gcggccgagg
     4021 gacaggcccg ttgacgccgc ggcaaccagc tgctgacccc agggacagga gccaggtgcc
     4081 aatgcctgac cgatgagcag ccaagagcgg ttcctccgct cgaggcgctc gagggcgcgt
     4141 cgagaccaag gtggctcccg ggtcacccga gaaa
//
LOCUS       DATCCH010000169         2169 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_102867, whole genome shotgun sequence.
ACCESSION   DATCCH010000169 DATCCH010000000
VERSION     DATCCH010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102867"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VIJ44_03390"
     CDS             <1..234
                     /locus_tag="VIJ44_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018657792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HEY5164977.1"
                     /translation="GVYCGAVGWIDTERDHGDLAVAIRTFTVTHGETRLGTGAGIVAD
                     SDPAREWEETELKVARLSRVAEGREAGAVRTPA"
     gene            complement(276..>2169)
                     /gene="infB"
                     /locus_tag="VIJ44_03395"
     CDS             complement(276..>2169)
                     /gene="infB"
                     /locus_tag="VIJ44_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEY5164978.1"
                     /translation="QRPRRKKRRRRDFEDLGPASPTALTPLDAPVPEGEIVVPRGITI
                     QELAPKFNRNAADLVRILMDAGEMVTGTQTLADEMIELIAETLSAEVLLVEPGQEQEL
                     ELQALLGDEEEEEDDALLEPRPPIVTVMGHVDHGKTTLLDAIRSTNVVAGEAGGITQR
                     IGAYQVDRDGRRITFIDTPGHEAFTAMRARGAQATDIAILVVAADDGVMPQTVEAISH
                     ARVAEVPVIVAITKVDREDADTQRVRQQLVERELVPEEWGGDTIVVEVAAPTGQGIED
                     LLAAIVLVADVEELTANPKAPARAFVLESNLDQGRGPVVTALVERGTLRVGEPFVAGN
                     GWGKVRALFDDHGEAVKEAVPSMPVEVLGLDELPLAGDELRVAPDEKTARTVAEARGR
                     RRRAAGMAHPLTLAGGARLEDIFAMVQRGEVATLNVVLKSDTQGSLEAMTDALRKLDQ
                     DHEEVRLSFVHRGVGGITVSDINLAAVSSATVIGYNVRPDRKSRELAESENVELRLYE
                     VIYQALEDVTAALVGMLQPEFEEVVTGDAEVREVFSVPRVGKIAGCYVQNGTITRGSK
                     VRFLREGVVIWNGTITSLKRFKDDAREVQAGFECGIGLSDFQDLKPGDVIETYDLNEI
                     ARTLSS"
BASE COUNT          294 a          668 c          826 g          381 t
ORIGIN      
        1 ggcgtctatt gcggcgcggt cgggtggatc gacacggagc gtgatcacgg tgatctcgcg
       61 gtggcgatcc gcacgttcac cgtgacgcac ggcgagacgc ggctcggaac cggcgccggg
      121 atcgtggccg actcggatcc tgcccgcgag tgggaggaga ccgagctcaa ggtggcccgg
      181 ctgtcccggg tggcggaagg ccgggaagcc ggcgcggttc gcaccccggc ctgagcggtc
      241 gggacgcgga cgaaggccgg gatgaaacgt cgcgctcagg aagagagggt tcgggcaatc
      301 tcgttgaggt cgtaggtctc gatgacgtca ccgggtttga ggtcctggaa gtcggacagg
      361 ccgattccgc actcgaaccc ggcttggacc tcgcgggcgt cgtccttgaa tcgtttgagc
      421 gaggtgatgg tgccgttcca gatgacgacg ccctcgcgca ggaaccggac cttcgagccg
      481 cgggtgatcg tcccgttttg gacgtagcag ccggcgatct tgccgacccg ggggacggag
      541 aagacctcgc ggacctcggc gtcgccggtg acgacttctt cgaactcggg ttggagcatg
      601 ccgaccagcg cggcggtgac gtcttcgagg gcctggtaga tgacctcgta gaggcggagc
      661 tcgacgttct cggactcggc gagctcgcgg gacttgcggt ccgggcggac gttgtagccg
      721 atgacggtgg cgctcgacac cgcagcgagg ttgatgtccg acacggtgat cccgcccacg
      781 ccgcggtgca cgaacgagag tcgaacctcc tcgtggtctt ggtcgagctt gcgcagcgcg
      841 tcggtcatgg cctcgaggga gccttgggtg tcggacttca ggaccacgtt gagggtggcg
      901 acctcgccgc gctgaaccat cgcgaagatg tcttcgaggc gagccccgcc ggcgagggtc
      961 aacgggtgcg ccatgccggc ggcgcgtcgg cggcgcccgc gggcctcggc gacggtgcgg
     1021 gcggtcttct cgtcgggggc gacgcggagc tcgtcgccgg cgaggggcag ctcgtcgagg
     1081 ccgaggacct cgaccggcat cgacgggacg gcttccttca cggcttcgcc gtggtcgtcg
     1141 aacagggcgc ggaccttgcc ccagccgttc ccggcgacga agggctcacc gacccgcagg
     1201 gtgccgcgtt cgacgagtgc ggtgacgacc gggcctcgtc cctgatcgag gttcgattcg
     1261 aggacgaacg cgcgagcggg ggccttcggg ttggcggtca gctcctccac gtcggcgacg
     1321 agcacgatcg cggcgagcag gtcctcgatg ccttggccgg tgggggcggc gacctccacg
     1381 acgatggtgt cacctcccca ctcttcgggg acaagctctc gttcgacgag ctgttggcgg
     1441 actcgttggg tgtcggcgtc ttcgcggtcg accttcgtga tcgcgacgat gaccgggacc
     1501 tcggcgaccc gggcgtggct gatcgcctcg acggtttggg gcatgacacc gtcgtcggcg
     1561 gcgacgacga ggatcgcgat gtcggtcgcc tgcgcgccgc gggcccgcat ggcggtgaac
     1621 gcctcatggc cgggggtgtc gatgaaggtg atgcggcggc cgtcacggtc gacctggtag
     1681 gcgccgatgc gttgggtgat gccgcccgct tcgccggcga cgacgttcgt ggagcggatc
     1741 gcgtcgagga gtgtcgtctt gccgtggtcg acgtgaccca tgacggtcac gatcggtggg
     1801 cgcggctcga ggagcgcgtc atcttcttct tcttcttcat cgccgaggag tgcctgcagc
     1861 tcgagctctt gttcctggcc gggctcgacg aggagcactt cggcgctgag cgtctcggcg
     1921 atcagctcga tcatctcgtc ggcgagggtc tgggtgccgg tgaccatctc gccggcgtcc
     1981 atcaggatcc gcacgaggtc cgcggcgttg cggttgaact tgggtgcgag ctcttggatt
     2041 gtgatccctc gtgggaccac gatctcgcct tcggggaccg gcgcgtcgag tggggtcagc
     2101 gcggtcggcg acgcgggccc gaggtcttcg aagtcgcggc gacgacgctt cttgcgacgc
     2161 ggccgttgc
//
LOCUS       DATCCH010000170         2563 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_185577, whole genome shotgun sequence.
ACCESSION   DATCCH010000170 DATCCH010000000
VERSION     DATCCH010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185577"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /gene="rplR"
                     /locus_tag="VIJ44_03400"
     CDS             <1..280
                     /gene="rplR"
                     /locus_tag="VIJ44_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEY5164979.1"
                     /translation="PRLAVFRSSRHVYAQVIDDVQGRTLVAASTMETDVRSSATANVE
                     AAKSVGQRVGERAKASGITQVVFDRGGFRYHGRVAAVADGARESGLEF"
     gene            364..900
                     /gene="rpsE"
                     /locus_tag="VIJ44_03405"
                     /pseudo
     CDS             364..900
                     /gene="rpsE"
                     /locus_tag="VIJ44_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004601426.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
     gene            903..1088
                     /gene="rpmD"
                     /locus_tag="VIJ44_03410"
     CDS             903..1088
                     /gene="rpmD"
                     /locus_tag="VIJ44_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEY5164980.1"
                     /translation="MATTLRITQVRSAIGTKPKQRGTLRALGLRRINHTVEHPDRPEI
                     RGMVDRVPHLVTVEEVA"
     gene            1094..1585
                     /gene="rplO"
                     /locus_tag="VIJ44_03415"
     CDS             1094..1585
                     /gene="rplO"
                     /locus_tag="VIJ44_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEY5164981.1"
                     /translation="MKVHDLKPPAGSKRPARRVGRGIAGKGGKTAGRGSKGQGARGSV
                     KPGFEGGQTPIARRTPKAKGFKNPFRVEYAVVNLEKLAGFDAGTTVDPDTLRDRGLVA
                     KRGLVKILGRGEIAVSLTVKAHAFSDAARRAIEAAGGSVEVLAPPWGDRRPAARGNAL
                     TNR"
     gene            1621..>2563
                     /gene="secY"
                     /locus_tag="VIJ44_03420"
     CDS             1621..>2563
                     /gene="secY"
                     /locus_tag="VIJ44_03420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEY5164982.1"
                     /translation="MLARLRNMFRVPDLRNKILFTLLIVAVYRVGAHLPVPFVDFKAI
                     KQLQSQANNSGVVGFLDLFSGGAITNVAIFFLGIMPYITASIIMQLLGVVIPKLEQWQ
                     QEGQTGQKKITQWTRYLTIALALVQSTGFVFALHAGSGGLFGLSASLPKGVDFIPHFD
                     TPRAALIVLTWTAGTALVMWLGELITQRGIGNGMSILIFASVVSRLPAQGGAIYSQGS
                     KFQFFTILGIGLAMIVGIVFVESGQRRIPVQFAKRVVGRRMYGGQSTYIPLKVNQSGV
                     IPVIFASSILSFPALIASALGTSAGGIQKWINDNLVAR"
BASE COUNT          440 a          862 c          849 g          412 t
ORIGIN      
        1 cccccgcctc gcggtgttcc ggtcgagccg ccacgtctac gcgcaggtga tcgacgatgt
       61 ccagggccgc accctcgtgg ctgcctccac gatggagacc gatgtccgtt cctccgccac
      121 cgcgaacgtg gaggcggcca aatcggtcgg ccagcgggtg ggcgagcgag ccaaggcctc
      181 cggcatcacc caggtggtgt tcgaccgggg cggcttccgc taccacggtc gggtcgccgc
      241 ggtcgccgac ggggcccgtg aatccggact ggagttctaa gtggagcaca ttcgcaatca
      301 ggacagacgc cgaacgccga tgaggcagcc ggcgtcgaaa gcaggtcggt aatggccgaa
      361 gggcagtacg aagagcgggt catcgcgatc aaccgcgtcg ccaaggtcgt gaagggtggt
      421 cggcggttct cgttcaccgc gctcgtcgtc gtcggcgacg gcgagggtca cgtcgggctg
      481 ggctacggca aggcgaagga ggtacccgcg gcgattcaga agggcatgga agaaggcaag
      541 aagaacctct tcagcgtcgc gctcgcgggt tcgacgatca cccatgtggt gctcggcgaa
      601 cacgacgcgg cccgggtcct gctgaagccg gcggcgccgg ggaccggcgt catcgctggg
      661 ggtgcggctc gcgccatcct cgaagccgcg ggcatccacg acgtgctggc gaagtcgctc
      721 gggtcgtcga acgcgatcaa cgtgtcccga gcaaccatcc aggggctgcg gtcgttgaag
      781 cgcccggatg aggtcgcgaa gctccgcggg aagtcgcctg aggaagtcag ctcagccggg
      841 atgctgcgcg cctatcgcga gcgtcaagcg cccaagcgcg agatcaccga ggtcaaatag
      901 ccgtggccac aaccctgcgc atcacacagg tccgctctgc gatcggaacc aagccgaagc
      961 agcgtggcac gctgcgcgcc ctcgggctac ggcgcatcaa ccacaccgtc gagcaccctg
     1021 accgaccaga gatccgcggc atggtcgacc gcgtccctca tctcgtcacc gtcgaagagg
     1081 tcgcctgatg ccgatcaaag tgcacgatct gaaaccaccc gcgggctcga agcgaccggc
     1141 tcgtcgtgtc gggcgtggaa tcgcagggaa aggcggcaag accgcggggc ggggctcgaa
     1201 gggccagggc gcgcgcggct cggtcaagcc cggtttcgag gggggccaga cccccatcgc
     1261 ccggcgcacg ccgaaggcaa agggcttcaa gaacccgttt cgtgtcgagt acgccgtcgt
     1321 caacctcgag aagctggctg ggttcgacgc gggcacgacg gtcgacccgg acacactgcg
     1381 agaccgtggt ctggtcgcca agcgtggtct cgtcaagatc ctcggacgtg gagagatcgc
     1441 ggtctcgctc acggtcaagg cccatgcctt ctctgacgcc gcccgccggg cgatcgaggc
     1501 ggccggtggc agcgtggaag tgctcgcccc gccgtggggt gaccgccgtc cggccgcccg
     1561 gggcaacgcc ctcaccaatc ggtaggctgt cggctctgcc ggctcaggag gcccctgcac
     1621 atgctcgccc ggcttcggaa catgttccgg gtccccgacc tccggaacaa gatcctcttc
     1681 acgctgctca tcgtggcggt ctatcgggtc ggcgcccacc tgccggtgcc gttcgtcgac
     1741 ttcaaggcga tcaagcagct tcagagccag gcgaacaaca gcggggtcgt cgggttcctc
     1801 gacctcttct ccgggggggc gatcaccaac gtcgccatct tcttcctcgg gatcatgccg
     1861 tacatcacgg catcgatcat catgcagctg ctcggtgtcg tcatcccgaa gctcgagcag
     1921 tggcaacagg aaggccagac tggccagaag aagatcaccc aatggactcg gtatctcacc
     1981 atcgcgctgg cgttggtcca gtcgaccggg ttcgtgttcg ccctccacgc cggcagcggc
     2041 ggcctcttcg gtctgagcgc cagcctcccg aagggcgtcg acttcatccc ccacttcgac
     2101 accccccggg cggcgctcat cgtcctcacg tggaccgcgg gcacggcgct cgtcatgtgg
     2161 ctcggtgagc tcatcaccca gcgggggatc gggaacggga tgtcgatcct gatcttcgca
     2221 tccgtcgtgt cgcggctccc cgcccagggc ggcgccatct acagccaggg cagcaagttc
     2281 cagttcttca cgatcctcgg tatcggtctg gcgatgatcg tcgggatcgt cttcgtcgaa
     2341 tcggggcagc gccgaatacc agtccaattc gcgaaacggg tcgtcggccg ccgcatgtac
     2401 ggcggccaga gcacctacat cccgctcaag gtcaaccagt ccggtgtgat ccccgtgatc
     2461 ttcgccagct cgatcttgtc cttccctgcg ttgatcgcgt cagcgctcgg aaccagcgcg
     2521 ggtgggatcc agaaatggat caacgacaac ctcgtggccc gcc
//
LOCUS       DATCCH010000171         3830 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694 k141_9661,
            whole genome shotgun sequence.
ACCESSION   DATCCH010000171 DATCCH010000000
VERSION     DATCCH010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3830
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9661"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1177
                     /locus_tag="VIJ44_03425"
     CDS             <1..1177
                     /locus_tag="VIJ44_03425"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEY5164983.1"
                     /translation="EVIAAYGADTVRAAVDWTIDDAHRRFASEIERWPDGRYEADVFV
                     DADPAGNEDIHVHVAVTVDGGRLVVDFDGSDRRPEIQAWSTFGNTRGYTIAQLASLVD
                     PSIPKNEGFFDCVELRVPEGTCVNPTEGKPVSSGTHHPGVEVGDAVALAMAQILPDRC
                     APQTYKYGSPRQMWGDLDPRTGQPFFDHGGEVNAGWVNAVRDVDGWGALVASNGNLIK
                     ASAEINEALFPHLLRGRNYLTDSGGAGQWRGGCGSEFVKEVRTPTSVNQYVVNQRHTH
                     PGIAGGRNGSPDLCVISAGTPREVTVAPSVAGVEMATGDTLVYRFGGGGGWGDPLARD
                     PAAVLDDVWDEYVSVDAAREEYGVVVTGSLEAMTLAVDEAATRELRARRSGRAEPRG"
     gene            1186..3282
                     /locus_tag="VIJ44_03430"
     CDS             1186..3282
                     /locus_tag="VIJ44_03430"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEY5164984.1"
                     /translation="MSEYRIGIDVGGTFTDLVLLRPDGSVVLEKTPTTPADQSEAVLA
                     GLARLASAEELDLAALLARTASIVHGTTTGDNTMIQMSGAPTGLLVTTGFRDEIELRR
                     CYKEDIWDPAYPAPQPIARRRVRLEIPERLTAEGVVDTPLDEDAVRRATRRLHAFGVT
                     SIAVVFLHSYLNPTHELRARDLITEEYPDVELVSLSHEVYPKPPEFERTSTTLVNAYV
                     GPPIVRYLERLERRLADAGYRRQLLIATSAGGVATPDAIGRRAVATIGSGPTGGVVAA
                     ARAARDAGLGDVVSVDMGGTSYDVCLIRDGRPELSSDWNWRHRYCIALPMVNIHAVGA
                     GGGSIARVDEGGVLHVGPESAGSDPGPACYGLGGTAPTVTDADVLLGRLDPAGFWGGR
                     RALEVDRAAEALERLGQELGVDAVEAAVAVLRIVDAHMADAIRRVLALAGADPRRLDL
                     VAFGGMGAVHAATQAAALGIRRLLVPRAAPGFSALGLMSADHVVDAARTLLADWRSVD
                     LVRLNVLADDVEGAARGELSAAGVPDSRVHLEWMLNLVYPGQTFDHSIALPRRTGTPI
                     TADDVAAAVEEFHRENEAARLIEARSQEPMVRGIRLAATGLVDQPTPVVLEPETKPAS
                     PVSERRVHTGDGWHDAPVYDGEHLRPGPPLTGPAIVQWRFTTLLLLPRDRAHVRPNGD
                     VLVEIAATTGSTPPVV"
     gene            complement(3292..>3830)
                     /locus_tag="VIJ44_03435"
     CDS             complement(3292..>3830)
                     /locus_tag="VIJ44_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164985.1"
                     /translation="AAVAVAVATVGSSVAGAATTAKGSGLSVGHSCTYLTAQQVQKVF
                     RSPVTIDPTNRGASVLVSSACSYLVGPPGQPTGALVALDLFPFFPPPGQTAIDALESQ
                     RATDSLGGLTIIDGKLGQKSYIDLDRSVIVVAPSKKFAFTLQWLPTGGPSAGGRLDTK
                     TQKQLTTLAKQVITRAPK"
BASE COUNT          549 a         1372 c         1360 g          549 t
ORIGIN      
        1 tgaggtcatc gccgcgtacg gtgccgacac cgtgcgagcc gcggtcgact ggaccatcga
       61 cgacgcgcac cgccggttcg cgtcggagat cgagcgttgg cccgacggcc ggtacgaggc
      121 ggacgtgttc gtcgacgccg acccggctgg gaacgaggac atccacgtcc atgtcgcggt
      181 caccgtcgac ggcggtcggc tcgtcgtcga tttcgacggc tcggaccgcc gacccgagat
      241 ccaggcctgg tcgacgttcg gcaacacccg cggctacacc atcgcccagc tggccagcct
      301 ggtcgacccg tcgatcccga agaacgaggg gttcttcgac tgtgtcgagc tgcgggtccc
      361 ggagggcacg tgcgtgaacc cgaccgaagg caagccggtc agcagcggca cccaccatcc
      421 cggtgtcgag gtcggtgacg ccgtcgcgtt ggcgatggcg cagatcctcc ccgaccggtg
      481 cgccccccag acctacaagt acgggagtcc ccgtcagatg tggggcgacc tcgatccccg
      541 caccgggcaa ccgttcttcg accatggcgg cgaggtcaac gcggggtggg tcaacgcggt
      601 gcgtgacgtc gacggctggg gggcgctggt cgcctcgaac gggaacctca tcaaagcctc
      661 ggccgagatc aacgaggcgc tgttcccgca cctgctgcgg ggccgcaact acctcaccga
      721 ttcgggtggc gcgggccagt ggcgcggggg ctgcggctcg gagttcgtca aggaggtccg
      781 cacacccacc tcggtcaacc agtacgtggt gaaccagcgc cacacccacc ctggcatcgc
      841 cggcggccgc aacggttccc ccgatttgtg tgtgatcagc gccggcaccc cccgcgaggt
      901 cacggtcgcc ccgtcggtcg cgggcgtgga gatggcgacc ggcgacacgc tggtgtaccg
      961 gttcgggggt ggtggcggct ggggggaccc gttggcccgt gaccccgcgg cggtcctgga
     1021 cgacgtgtgg gacgagtacg tgtccgtcga cgccgcccgc gaggagtacg gggtcgtggt
     1081 gaccggctcg cttgaggcca tgacgctcgc cgtcgacgag gccgccaccc gcgagctgcg
     1141 ggcgcgtcgc agcggccgcg ccgaaccccg gggctgaacg cggcggtgag cgagtaccgc
     1201 atcgggatcg atgtcggcgg gacgttcact gacctcgtgc tcctccgccc ggacgggtcg
     1261 gtggtgctcg agaaaacacc gacgacacca gccgaccagt ccgaagcggt cctggccggt
     1321 ctcgctcgtc tcgccagcgc ggaggagctc gacctcgcgg cgctgctcgc ccgcaccgcg
     1381 tcgatcgtgc acggcaccac gacgggcgac aacacgatga ttcagatgtc gggcgcaccg
     1441 accggtctgc tcgtcaccac cgggttccgt gacgagatcg agctgcgccg ctgctacaag
     1501 gaggacatct gggatccggc gtacccggca ccccagccga tcgcccgccg gcgggttcgc
     1561 ctcgagatcc ccgagcgtct gaccgccgaa ggggtcgtcg acacacccct cgacgaggac
     1621 gcggtccgac gcgccacccg gcgcctccac gcgttcgggg tcacgtcgat cgcggtcgtg
     1681 ttcctccact cctacctcaa cccgacgcac gagcttcgcg cccgcgacct catcaccgag
     1741 gaataccccg acgtcgagct ggtgtcgctg tcccacgagg tctacccgaa gccgccggag
     1801 ttcgagcgca cgtcaacgac cctggtgaac gcctacgtcg ggccgccgat cgtccgctac
     1861 ctcgagcgcc tcgaacgccg cctcgctgac gccggatatc gccggcagct gctcatcgcc
     1921 acgtcggcgg gtggtgtcgc cacccccgac gcgatcgggc ggcgcgcggt ggcaaccatc
     1981 gggtccgggc cgaccggcgg cgtggtggcc gcggcgcgtg cggcccgcga cgccggtctc
     2041 ggcgatgtgg tgagcgtcga catgggtggc accagctacg acgtctgtct catccgcgac
     2101 ggacgacccg agctgagcag cgactggaac tggcgtcacc gatactgcat tgcgctgccg
     2161 atggtgaaca tccacgccgt cggcgccggc ggcggttcga tcgcccgggt cgacgagggt
     2221 ggtgtcctcc acgtcggacc ggagtccgcc gggagcgacc ccggcccggc gtgttacggg
     2281 ctcggaggca cggcaccgac ggtgaccgac gccgacgtgc tcctcggtcg gctcgacccg
     2341 gcggggttct ggggcggacg acgcgccctc gaggtcgacc gcgccgcgga ggcgctcgaa
     2401 cggctaggcc aggagctcgg tgtcgacgcg gtcgaggccg ccgtcgcggt gctgcgaatc
     2461 gtcgacgcgc acatggccga cgcgatccgt cgcgtgttgg ccctcgccgg cgctgacccg
     2521 cgccgcctcg acctcgtcgc cttcggcggg atgggcgcgg tgcacgccgc gacccaagcc
     2581 gccgcgctgg ggatccgccg cctgctcgtc ccccgggccg cgccgggctt ctcggcgttg
     2641 gggctgatga gcgccgacca cgtcgtcgac gccgcccgga ccctgctcgc cgactggcgg
     2701 tccgtggacc tggttcgcct caacgtgctc gccgacgacg tcgagggtgc ggcgcgcggc
     2761 gagctcagcg ccgccggcgt gcccgacagc cgggtgcacc tggagtggat gctgaacctc
     2821 gtgtaccccg gccagacgtt cgaccactcg atcgcgctcc cccgtcggac cggcaccccg
     2881 atcacggccg atgacgtggc cgccgcggtc gaggagttcc accgcgagaa cgaggcagcc
     2941 cgactcatcg aagcccgctc gcaggagccg atggtgcgag ggattcgtct cgccgcgacc
     3001 gggctggtcg accagccaac cccggtggtg ctcgagccgg agacaaagcc ggcctcgccc
     3061 gtcagtgaac gccgcgtcca caccggcgac gggtggcacg acgcgcccgt ctacgacggc
     3121 gaacacctgc gaccggggcc gcccctgacc ggaccggcga tcgtgcagtg gcggttcacg
     3181 acgctgctgc tcttgcctcg ggaccgcgcc cacgtccgac cgaacggcga tgtcctggtc
     3241 gagatcgccg ccacgaccgg ttcgacccca cccgtggtgt agcccttcgc cctacttggg
     3301 ggcgcgggtg atgacctgct tcgcgagggt cgtgagctgc ttctgggtct tggtgtcgag
     3361 gcgcccaccg gcggatggtc cgccggtcgg tagccattgg agggtgaacg cgaacttctt
     3421 gctcggcgcg accacgatga ccgaccggtc gaggtcgatg tagctcttct ggcccagctt
     3481 gccgtcgatg atggtcagcc cgcccagcga atcggtggca cgttggctct cgagcgcgtc
     3541 gatggccgtc tgaccgggtg gagggaagaa cgggaacagg tcgagcgcga cgagcgcgcc
     3601 ggtgggctga cccggaggcc ccacgagata gctgcaggcc gaggagacca ggacgctggc
     3661 accccggttg gtcgggtcga tggtgaccgg cgacctgaac accttctgga cctgctgggc
     3721 ggtcaggtag gtgcacgaat gcccgacgct cagccccgag cccttcgcgg tcgtcgccgc
     3781 gcccgcgacg ctggacccga cggtcgcgac cgcgaccgcg accgcagcaa
//
LOCUS       DATCCH010000172         4695 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_341984, whole genome shotgun sequence.
ACCESSION   DATCCH010000172 DATCCH010000000
VERSION     DATCCH010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4695
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341984"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1985
                     /gene="ppk1"
                     /locus_tag="VIJ44_03440"
     CDS             <1..1985
                     /gene="ppk1"
                     /locus_tag="VIJ44_03440"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEY5164986.1"
                     /translation="ALAEDSTRPLLERAKFLAIFSINLDEFFQVRVSGLQEQVAAGIR
                     TRSPDGLDPLETIRAIREQVRTLVERQTTIFTKDVAPGLEEAGIRFERWDDLDDADRR
                     EMQATFEEHVYPVLTPLAVDPAHPFPYISNLSLNLAVVVRDPETSGQRFARIKVPPLL
                     PRFVPLSDGFRFVPLEQVIAAHLDRLFPGMELLDRVPFRVTRDADFELDDEDEDLLEA
                     IESVLHRRSKFGHAVRLEVDPSITDEVLDVLCRELEIPPAAVVSIDGPLDLSGLWDIC
                     ALERPDLKDEAWLPQTPPALVPLGDETPDLFRLLRDGDVLVHHPYDSFATSVEAFVDQ
                     AASDPNVLAIKQTIYRTAGPGSAIVRSLVDAARQGKQVVALVELKARFDEQANIERAR
                     VLEEAGVHVVYGLVGLKTHCKILLVVRQEDDGIRRYCHVGTGNYNPQTATLYEDLGLL
                     SADPALGADLTELFNHLTGYSRSVRYRRLLVAPIGLRPGLRDLIRREAARGDRGRIVM
                     KMNSLVDPELIDELYAASKAGTDIDLIVRGICCLRPQVPGRSERIRVRSLVGGFLEHS
                     RIFRFGSSPAAEYLIGSADLMPRNLDRRVEALCPVDAPSLRTRLDEILDTNLADDVLS
                     WELAPDGEWHKIETVAGVNAQARLRALAVERAQIRT"
     gene            1982..3478
                     /locus_tag="VIJ44_03445"
     CDS             1982..3478
                     /locus_tag="VIJ44_03445"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH and CHAD domain-containing protein"
                     /protein_id="HEY5164987.1"
                     /translation="MTVPEREIKLAAPPTVTLPPLSDPLAGVIAEPAGTRHLVATYYD
                     TDDLRLARSGVSLRHRSDEGWAVQLPATSDGDPLLDWSTYAFAGDEADPPPEALDLVR
                     AFVRTATVTEVARLRTVRRAVHLRTAQGSPIGDVVDDDVVVVGGDAPVRAFRELEIEV
                     ADGAPEAQTTAIVARLRAAGAGAPDPTPKIVRALGPRAAEPPDIEPVSGSARGLHEVV
                     SDALRASVAKLVTHDAGARRGVDAEELHQARVATRRLRAHLRTFRPLLEEHWADALRL
                     DLQWLGAALGAVRDADVLLERLEGRLTHVPAQDQSDGERLLHFLGAQRAEARQDLLVV
                     LRSDRYAELLERLVQAARAPRLLARVGAETDEDLLRAAARRPWDDLCQTIESLPDEPT
                     DEELHAVRRRAKQARYAAEAVEPAFGKPARNFARALTEVQDVLGEHQDGVVAATWLRT
                     AARQVHDGHAAFVAGELVALEHETALAARRTWSASWQRASRKKHHQWL"
     gene            3469..3957
                     /locus_tag="VIJ44_03450"
     CDS             3469..3957
                     /locus_tag="VIJ44_03450"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013424818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY5164988.1"
                     /translation="MAVSKTEPGGEPSQATVRAAGGIVVRPLAGRSRIRQRLRPRHEI
                     ALVHRPRYDDWSFPKGKPDGEERDEDTALREVQEETGLGCRLGTPVGETRYQDSRGRD
                     KVVRYWLMEPPEGDPAAAFVPNREVDALRWLEPDEADRLLTYQHDRELLHRLSRRGPP
                     RP"
     gene            3954..4451
                     /locus_tag="VIJ44_03455"
     CDS             3954..4451
                     /locus_tag="VIJ44_03455"
                     /EC_number="5.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HEY5164989.1"
                     /translation="MTPATQMYLVRHAKAGSRSDWPGPDDLRPLTKAGRRQADALRDL
                     LADRGVTQVVSSPYVRCRQTVEPLGQRLRLPVDLADELAEGTPLPEARRLIEKFTSEA
                     TVLCTHGDVIGELLESLARQGLIGRDIRLEKGSTWVLDFQGDEITGATYLAPPELTVE
                     ARVRR"
BASE COUNT          714 a         1761 c         1575 g          645 t
ORIGIN      
        1 tcgcgctcgc cgaagattcg acacgcccgt tgctcgaacg cgccaagttc ttggccatct
       61 tctcgatcaa cctcgacgag ttcttccagg ttcgcgtctc gggtttgcaa gagcaggtcg
      121 cagcgggtat ccgcacccgg tcgccggacg gcctcgaccc gctcgagacg atccgcgcca
      181 tccgcgagca ggtgcgaacg ctcgtcgagc gtcagaccac gatcttcacc aaggacgtcg
      241 cgcccgggct cgaggaggcg gggatccgct tcgagcgctg ggacgacctc gacgacgccg
      301 accgccgcga gatgcaggcg acgttcgagg agcacgtgta cccggtcctc accccgctgg
      361 cggtcgaccc ggcgcacccg tttccttata tctcgaacct gtcgctgaac ctggcggttg
      421 tcgtccgcga ccccgagacg agcgggcagc gcttcgcccg catcaaggtc ccgccgctgc
      481 tcccacggtt cgtgccgctg tccgacgggt tccggttcgt tcccctcgag caggtgatcg
      541 ccgcgcacct ggaccggctg ttccccggga tggagctgct ggaccgggtg ccgttccggg
      601 tgacccgcga cgccgacttc gagctcgacg acgaggacga ggacctcctg gaggcgatcg
      661 agtcggtcct gcatcgacgt tcgaagttcg ggcacgcggt gcgactcgag gtcgacccgt
      721 cgatcaccga cgaggtcctc gacgtcttgt gccgcgagct cgagatcccg cccgcggcgg
      781 tggtgtccat cgacgggccg ttggatctca gcggcctgtg ggacatctgc gcgctcgagc
      841 ggcccgacct caaggacgag gcgtggctcc cccagacccc gccggcactc gtgcccctcg
      901 gcgacgagac ccccgacctg ttccggctgc tccgcgacgg tgacgtgctg gtgcaccacc
      961 cctacgactc gttcgcgacg tcggtggagg cgttcgtcga ccaggccgcc agcgacccga
     1021 acgtgttggc gatcaagcag accatctacc ggaccgcggg tccgggcagc gccatcgtgc
     1081 ggtcgctcgt cgacgcggcc cgtcaaggca agcaggtcgt agcgctggtg gagctcaagg
     1141 cccgcttcga cgagcaggcc aacatcgaac gggcccgggt gctcgaagaa gcgggcgtgc
     1201 acgtcgtgta cgggctggtc ggcctcaaga cccactgcaa gatcctgctc gtcgtccgcc
     1261 aggaagacga cgggatccgc cggtactgcc atgtcggcac cgggaactac aacccccaga
     1321 ccgcgacgct gtacgaggac ctggggctcc tctccgccga cccggcgctc ggcgcggacc
     1381 tcaccgagct gttcaaccat ctcaccggat acagccgatc ggtccgctac cgccggctgc
     1441 tcgtcgcgcc gatagggctg cgaccggggt tgcgcgacct gattcgacgc gaggcggccc
     1501 gaggtgaccg gggacgcatc gtcatgaaga tgaacagcct ggtcgacccg gagctcatcg
     1561 acgagctgta cgccgcgtcg aaggccggca ccgacatcga tctcatcgtc cggggcatct
     1621 gctgtctgcg gccccaggtg ccgggacggt cggagcgcat ccgggtgcgc tccctcgtcg
     1681 gggggttcct cgagcactcc cgaatcttcc gcttcgggtc gagccccgcg gcggagtacc
     1741 tgatcggctc ggcggacctc atgccccgga acctggaccg gcgcgtcgag gcgctctgcc
     1801 cggtcgacgc accttcgttg cggacccgcc tcgacgagat tctcgacacg aacctcgccg
     1861 acgacgtcct gtcgtgggaa ctcgcccccg atggcgagtg gcacaagatc gagaccgtgg
     1921 ccggcgtcaa cgcccaggcc cggctgcgtg cgctcgccgt tgagcgagct cagatccgca
     1981 cgtgaccgtc cccgagcgcg agatcaagct ggcggcaccg ccgacggtga cgctcccacc
     2041 gttgtcggac ccgctcgccg gcgtcatcgc cgaaccggcc ggtacccgcc atctggtcgc
     2101 cacctactac gacaccgacg acctccgcct ggcccgatcg ggcgtgtcgc tgcgtcaccg
     2161 cagcgacgag ggctgggcgg tgcagctccc cgcgacgagc gacggtgacc cgctcctcga
     2221 ctggtcgacg tacgcgttcg ccggcgacga ggccgacccg ccccccgagg cgctcgatct
     2281 ggtccgggcg ttcgtccgca ccgcaacggt gaccgaggtg gcgcgcctgc gcaccgtccg
     2341 gcgggctgtg cacctgcgaa cggctcaggg aagcccgatc ggcgacgtcg tggacgacga
     2401 cgtggtcgtg gtgggcggcg acgcccccgt ccgggcgttc cgtgagctcg agatcgaggt
     2461 cgccgacggc gcccccgagg cccagaccac cgcgatcgtc gctcgactgc gcgccgctgg
     2521 cgccggcgcc cccgacccga cacccaagat cgtgcgggcc ttgggtcccc gagccgccga
     2581 gccgcccgac atcgagcccg tctcgggatc agcgcggggt ctgcacgagg tcgtgagcga
     2641 cgcgctccgg gcctcggtcg ccaaactcgt gacccatgac gccggcgccc gccgcggcgt
     2701 cgacgccgaa gagctccatc aagcccgcgt cgcgacgcgg cgcctgcggg cccacctgcg
     2761 aacgttccgg cctctcctcg aggaacattg ggccgacgcg ctccgcctcg acctccaatg
     2821 gctcggcgcc gcgctcggcg cggtgcgcga cgccgacgtc ctcctcgagc gtctcgaagg
     2881 gcgcctcacc cacgtccccg cccaagacca gtccgacggc gaacgcctcc tccacttcct
     2941 cggggcccag cgggccgagg cccgccagga cctgctggtg gtgctgcgca gcgaccggta
     3001 cgccgagctg ctcgagcggc tcgtgcaggc cgcccgagcg ccgcggctcc tcgcccgggt
     3061 gggagccgag accgacgagg acctgctccg tgccgccgca cgccgcccct gggacgatct
     3121 gtgtcaaacg atcgagtcgc tccccgacga accgaccgac gaggagctgc acgcggtgcg
     3181 ccgacgagcc aaacaggccc ggtacgcggc cgaagccgtc gagcccgcct tcggcaagcc
     3241 ggcccgcaac ttcgcgcgcg cgctcaccga ggtccaagac gtcctcggtg agcaccaaga
     3301 cggcgtcgtc gccgccacgt ggctccgcac cgcggcgcga caggtccacg acgggcacgc
     3361 cgcgttcgtc gccggggaac tcgtcgccct cgagcacgag accgccctcg ccgcgagacg
     3421 gacgtggtcg gcgtcgtggc agcgagcgag tcgcaagaag caccaccaat ggctgtgagc
     3481 aagaccgagc cgggcggcga gccgagccaa gccaccgtgc gcgccgcggg tggcatcgtc
     3541 gtgcgcccgc tcgcgggtcg gtcccggatc cgccaacgac tccgcccccg tcacgagatc
     3601 gcgctcgtgc atcggccccg ctacgacgac tggagcttcc cgaagggcaa gcccgacggc
     3661 gaggaacggg acgaggacac cgcgctccgg gaggtgcaag aagagaccgg cctgggctgc
     3721 cggctcggga ccccggtcgg cgagacccgc taccaggaca gccgaggccg cgacaaggtg
     3781 gtccgctact ggctcatgga accccccgag ggcgacccgg cggcggcgtt cgtccccaac
     3841 cgggaggtcg acgcgctccg ctggctcgaa ccggacgagg ctgacagact cctcacgtac
     3901 cagcacgacc gagagctgct ccatcgtctt tcccggcgag gtccgccacg accgtgaccc
     3961 cagcgacgca gatgtacctg gtgcgtcacg cgaaggccgg aagccgcagc gactggcccg
     4021 gtcccgacga cctgcgtccc ctcaccaagg ccggacggcg ccaagccgac gcgctccgtg
     4081 acctgctcgc cgaccgcggc gtcacccagg tggtcagcag cccgtacgtg cgctgccgcc
     4141 aaaccgtcga acccctcggc cagcgccttc ggctcccggt cgacttggcc gacgagctcg
     4201 ccgagggcac accactgccc gaagcccgcc ggctgatcga gaagttcacc agcgaagcga
     4261 cggtgctgtg cacccacggt gacgtgatcg gcgagctgct cgagtccctc gcccggcagg
     4321 gcctgatcgg ccgcgacatc cggttggaga agggctccac ctgggtcctc gacttccaag
     4381 gcgacgagat caccggcgcc acctacctcg cgccgcccga gctgacggtg gaggcccggg
     4441 tccgacggtg aacgtcgcgg cacggacgcg tgaccgtccg ggcttgagtg ggtgaacact
     4501 tcgggctggc ccttatcggc gcgagccgag cgacgagagt ggtggacggt gagcaccacg
     4561 atcatcacca cccggtttca ggactgcgac gagctcctcg ccgtcctgag ggccgccgac
     4621 gccgcccatc acggcgagtc ggtgagcctc ctcgcccaca gcctccagtg cgcagcgatc
     4681 ctcgaacgcg tcgct
//
LOCUS       DATCCH010000173         2188 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_558731, whole genome shotgun sequence.
ACCESSION   DATCCH010000173 DATCCH010000000
VERSION     DATCCH010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558731"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VIJ44_03460"
     CDS             <1..387
                     /locus_tag="VIJ44_03460"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164990.1"
                     /translation="TMSARGVDVSSHRSRPLTAALVADADLILAMTRSHVWGIHAFDP
                     EAAARTFLVVELPRLGARIGPRHSDETVRGWAARAAAERVGVLPGRGEEEIADPYGEP
                     ASVYEATAARLDAATRAIAELLVPDF"
     gene            complement(384..884)
                     /locus_tag="VIJ44_03465"
     CDS             complement(384..884)
                     /locus_tag="VIJ44_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5164991.1"
                     /translation="MVEHREAPDGPRLAFAHVDDFPWTEVIAQQHGDRRVSVHEKFLE
                     WTPQRMVVLGRYDPGMIIERHGHGSDNLVYVMEGDLTVGDRPCPAGTLITLEHGAVFG
                     PLIAGPDGCLLFETWGADVAPVPADKDGYARLLAERGVVRLPNPRFEKPPTAPRSDLG
                     TGDRWS"
     gene            947..1702
                     /locus_tag="VIJ44_03470"
     CDS             947..1702
                     /locus_tag="VIJ44_03470"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5164992.1"
                     /translation="MELEGKVAVVTGAGSGIGRATALAFARAGATVVAADIDEATAAE
                     TVGLVHDAGGQASEVRTDVTEPRSLEAMFTAGEAAHGGVDIVFNNAGLVCGEPLWPDI
                     TPETLLRVMAVNLGGVVVGTRLAIPVLRRRGGGAIVNTASMAALFPLTADPIYSATKA
                     GVAMFTRACAPLAEEGIRVNAVLPGLVDTPLLPKSGDGERWADWALAAQAVMGLLSPD
                     DVAAAVLELVRDDSAVAQERVVGGLPTVPEAPS"
     gene            1702..>2188
                     /locus_tag="VIJ44_03475"
     CDS             1702..>2188
                     /locus_tag="VIJ44_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017836002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEY5164993.1"
                     /translation="MVVSDETLTPNQAARRDRVLDAAMSLAAEGGYDAVQMRDVATRA
                     QVALGTIYRYFSSKDHLLAACQVELWRDLADRFNRRPPAGHTAADRVIELLDRAMQAA
                     EHEPRRTAALVTASASPDPAVRDCQIQLMATMDDVLAGAMGDLDPTVKARAARTLRMV
                     WF"
BASE COUNT          308 a          780 c          775 g          325 t
ORIGIN      
        1 acgatgtcgg cgcggggggt tgacgtctcg agtcaccgca gccgaccctt gaccgccgcg
       61 ctcgtcgccg acgccgacct gatcctcgcg atgacccgca gtcacgtctg gggaatccac
      121 gccttcgatc cggaggccgc ggctcgaacc ttcctcgtcg tggagcttcc ccgtctcgga
      181 gcccggatcg gaccccgaca ctccgacgag acggtgcgag gctgggcggc gagggccgcg
      241 gccgaacgcg tcggcgtgct ccccggtcgg ggcgaggagg agatcgccga cccgtacggg
      301 gaaccggcat cggtgtacga ggcgaccgcg gcgcgcctcg acgccgcgac gcgcgccatc
      361 gctgagctgc tggttccaga cttctaggac cagcggtctc cggtgccgag atcgctccgc
      421 ggcgcggtcg gcggtttctc aaagcgaggg ttcgggagtc gcaccacgcc ccgttccgcg
      481 aggaggcgcg cgtagccgtc cttgtctgcg ggtacggggg cgacgtcggc accccacgtc
      541 tcgaacagca agcagccgtc gggaccggcg atcaacggac cgaacaccgc gccgtgctcg
      601 agggtgatga gcgtccccgc cgggcacgga cggtccccga cggtcaggtc gccttccatc
      661 acgtagacga ggttgtcgct gccgtgaccg tggcgctcga tgatcatccc ggggtcgtag
      721 cgacccagga ccaccatccg ctggggcgtc cactcgagga acttctcgtg caccgagacc
      781 cggcggtcac cgtgctgctg cgcgatcacc tcggtccagg ggaagtcgtc gacatgcgcg
      841 aaggcgaggc gggggccgtc cggggcctcg cggtgctcta ccacgccacg agcgtaggcc
      901 gacgtggtgg aacgtgttct cgtagtcgtg ggactatggc ggtctcgtgg agctcgaagg
      961 caaagtcgcc gtcgtcaccg gagcggggtc gggcatcggt cgggccaccg cgttggcgtt
     1021 cgcgcgcgcg ggcgccacgg tcgtcgctgc cgacatcgac gaggcgactg ccgccgagac
     1081 ggtcgggttg gtccacgacg ccggaggcca ggcctcggag gtgcgcaccg acgtgaccga
     1141 gccccggtcg ctcgaggcca tgttcaccgc cggcgaggcc gcgcacggcg gagtggacat
     1201 cgtgttcaac aacgccgggc tggtctgcgg cgagccgctc tggcccgaca tcacccccga
     1261 gacgctgctg cgagtgatgg ccgtcaacct ggggggtgtc gtggtcggca ccaggctcgc
     1321 cattcccgtg ctgcgacgcc gcggcggggg cgccatcgtc aacaccgcgt cgatggcggc
     1381 gttgttcccc ctgaccgcgg atccgatcta ctcggccacg aaggcgggcg tggcgatgtt
     1441 cacccgggcg tgcgccccgc tcgccgagga gggcatccgc gtcaacgcgg tccttcccgg
     1501 tctcgtcgac acccccctgc tccccaaatc cggggacggg gaacgctggg ccgactgggc
     1561 tctggctgcg caagcggtca tgggtctgct ctcacccgac gacgtcgctg ctgcggtcct
     1621 cgagctggtt cgcgacgaca gcgccgtggc gcaggaacgc gtcgtcggag gcttgcccac
     1681 cgtcccggaa gcgccctcct gatggttgtc agcgacgaga ccctcacccc caatcaggcc
     1741 gctcgccgcg atcgggtgct cgacgccgcg atgagccttg ccgctgaggg gggctacgac
     1801 gcggtgcaga tgcgtgacgt cgccacccgg gcccaggtcg cgctgggcac catctaccgc
     1861 tacttcagct ccaaagacca tctgctggcg gcgtgtcagg tggagctgtg gcgagacctc
     1921 gccgaccggt tcaaccgccg cccaccggcc ggtcacaccg ccgccgaccg ggtcatcgag
     1981 ctcctcgacc gggcgatgca ggccgcggag catgagcccc gtcgaaccgc ggcgctcgtc
     2041 accgcatcag cgtcgccgga ccccgcggtc cgggactgtc agatccagct catggccacc
     2101 atggatgacg tgctcgccgg ggccatgggt gacctcgacc ccacggtcaa ggcccgcgcc
     2161 gcccgcacgc tccggatggt gtggttcg
//
LOCUS       DATCCH010000174         4155 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694 k141_9784,
            whole genome shotgun sequence.
ACCESSION   DATCCH010000174 DATCCH010000000
VERSION     DATCCH010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4155
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9784"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..813)
                     /locus_tag="VIJ44_03480"
     CDS             complement(28..813)
                     /locus_tag="VIJ44_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020553511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HEY5164994.1"
                     /translation="MGKIRYGARSEEELRNRELEATSSGAWSTTMVAIYETDPDVIAA
                     VLPPPLEPPDEPLARVTIATVDMGRPGIPVFGAATVAVMARHEGTGGDYALVMPMTTE
                     QSVIGGRETFGEPKKIADVTLERDGDRIVGKVTRLGTTFVEVSGQVTETLANPPDGRR
                     TSFYFKFLPSPDGKGFDAEPSLVYCHRDETTRTLERVDGTVLLRESRFDPVADIPVRR
                     IREITLAERRSRQVGEIHSRVPGDWLLPYVHQRYDDLSPVGEE"
     gene            complement(877..2154)
                     /locus_tag="VIJ44_03485"
     CDS             complement(877..2154)
                     /locus_tag="VIJ44_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019060669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5164995.1"
                     /translation="MRNAQPSSTNRYTVVSADGHAGAEIHDYRDYLEHAYLDEYDRWV
                     ADYVVPFPDLEGPNASRNWDSARRLAELEADGIVAEVLFPNTVPPFYPKSSLTQLPPA
                     DTAELGRRWAGLRAHNRWLADFCAATPGRRAGIAQILLNDVDLALAEIRWAHDAGLTG
                     GVLLPGAPPGTGLEPLYSPVYEPIWAVCEELGLPLNHHAGSAVPPMGDQPVDKVIFLL
                     EVTWWAHRTLWHLIVGGALERHPDLQLVFTEQGTAWIPERLATLDYFFERMRGGAGAT
                     GSQEVEWGATVMAPLSLQPSEYWARQCHVGSSFMRPSEVPLRYAVGVDKIMWGCDYPH
                     RETSFPYSREALRLAYADVDPTEVQAMVGANAAALYGFDLDRLAPIAAHVGPTVDEIA
                     RQLPPDDIPADARRCPAFADPPAARPSPIAAKT"
     gene            2214..2843
                     /locus_tag="VIJ44_03490"
     CDS             2214..2843
                     /locus_tag="VIJ44_03490"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEY5164996.1"
                     /translation="MPGPARQPGLSRDQIVGVALEIVEAEGLDGLTMRRLAGKLGVAA
                     TAIYWHVGDKEALLDALAERIAARVGDIPVAGDSPVERLVSIGAGLRRNLLEQPTLVG
                     LVHQQGHTALLFQPVRRALVRELTAAGLRGPATALALHVILSHISGSVLNDVQIERAP
                     EQHETPEELWAADEVLDDPELRDALARPVDRDELFDYSLTALAHALIDQ"
     gene            complement(2956..>4155)
                     /locus_tag="VIJ44_03495"
     CDS             complement(2956..>4155)
                     /locus_tag="VIJ44_03495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HEY5164997.1"
                     /translation="DDPDFNLEYHVRHTALPAPGGEPELRRLVGRVMSQQLDRTKPLW
                     ELWMVEGLGNNRWALLCKTHHSIVDGVSGTELLTVLLDTSPDAAAAADESWHPAPAPT
                     PLALATDAMATMARSPYEQLRALRAASRVPRQAAAQLGEICTGLAALAGLVPRTPASS
                     LNGPIGPHRRYGWASTTVDDIKRIRKELGGTFNDVVLTAITAGFRALLTSRGETVDRV
                     IRTLVPVSVRSRDLSGRAVGDGTYDNKVSAMFAELPVGIADPAERLQAVSTQLAGLKE
                     SNQAVAGEALTSLSGFAPPVLLALGTRIATKAAQRNINTVTTNVPGPQHRLYVLGRRM
                     LTAFPYVPLGAQCRIAVAIFSYDGQVNFGATGDYDTAPDLDVLCRGIERGMAELGTLA
                     SSAPAAS"
BASE COUNT          584 a         1346 c         1556 g          669 t
ORIGIN      
        1 agctcctcga tcacggtgtc gctcgactca ctcctcgccc acgggcgaga ggtcgtcgta
       61 gcgctggtgc acgtagggga gcagccagtc accggggacg cgggagtgga tctctccgac
      121 ctggcggctg cggcgctcgg ccagcgtgat ctcgcggatc cgccggaccg ggatgtcggc
      181 gactgggtcg aagcgcgatt cgcggagcag cacggtgccg tcgacgcgct cgagcgtgcg
      241 ggtggtctcg tcgcggtggc agtacacgag cgatggctcg gcgtcgaacc ccttgccgtc
      301 gggggagggg aggaacttga agtagaagct cgtgcgtcgg ccatcgggcg ggttcgccag
      361 cgtctcggtg acctgtccgc tgacctcgac gaacgtcgtc ccgagacggg tgacctttcc
      421 gacgatgcgg tctccgtcgc gttcgagcgt gacatcggcg atcttcttgg gctcgccgaa
      481 cgtctcgcgc ccgccgatca cggactgctc ggtagtcatc ggcatcacca gcgcatagtc
      541 gccgccggtg ccttcgtgac gggccatcac ggcgacggtt gcggcaccga agacggggat
      601 gccgggccgg cccatgtcga cggtcgcgat cgtcacgcgg gcgagcggtt cgtcgggcgg
      661 ctcgagcggc ggcggcagca ccgccgcgat cacgtccggg tcggtctcgt agatcgccac
      721 catcgtcgtc gaccacgccc cgctcgacgt cgcctcgagc tcacgattgc gcaactcctc
      781 ctcggatcgt gcgccgtatc ggatcttccc catgatcgtc tcccttgttc tgtggatcgg
      841 tggctcgcac gcgggttggt aaacccaacg ccgacgtcag gtcttggccg caatcggtga
      901 tggccgggcc gcgggcggat cggcgaatgc ggggcagcgg cgcgcgtcgg ccgggatgtc
      961 atcgggcggg agctgccgcg cgatctcgtc gacggtcggc ccgacgtgcg ccgcaatggg
     1021 cgcgagccgg tcgaggtcga agccgtacaa cgccgccgcg ttcgcaccga ccattgcctg
     1081 cacttcggtc gggtcgacgt ccgcgtacgc gagccgcagc gcctcccgtg aatacgggaa
     1141 gctggtctcg cggtgcgggt agtcacaccc ccacatgatc ttgtcgacac cgaccgcgta
     1201 ccgcagcggc acttcggagg gtcgcatgaa gctcgagccg acatgacatt ggcgggccca
     1261 atactcgctg ggctgcaacg acaacggggc catgacggtc gcgccccact ccacttcttg
     1321 tgagccggtg gctccggcac cacctcgcat ccgctcgaag aagtagtcga gtgtggcgag
     1381 tcgctcgggg atccaggcgg tgccctgctc ggtgaacacg agctggagat ccgggtggcg
     1441 ctccagcgcg ccgccgacga tgaggtgcca gagggtgcga tgcgcccacc aggtcacttc
     1501 gagcaagaag atgaccttgt cgacgggctg gtcacccatc ggggggacgg cgctgccggc
     1561 gtgatggttg aggggcaggc cgagctcttc gcacacggcc cagatcggct cgtagaccgg
     1621 cgagtagagg ggctcgagac cggtgccggg tggcgcgccg ggcagcagga cgccgccggt
     1681 gaggccggcg tcgtgcgccc agcggatctc tgccaatgcg agatcgacgt cgttgaggag
     1741 gatctgggcg atgcccgccc gccgacctgg tgtcgcggcg cagaagtcgg cgagccagcg
     1801 gttgtgggcg cgcagcccgg cccagcggcg tccgagctcg gccgtgtcgg ctggcggcag
     1861 ctgggtgagc gacgactttg ggtagaacgg tgggacggtg ttggggaaga gcacctcggc
     1921 gacgatgccg tcggcttcca gctcggcgag acgccgggcg ctgtcccaat tgcggctggc
     1981 gttgggccct tcgaggtcgg ggaagggcac gacgtagtcg gcgacccaac ggtcgtactc
     2041 gtcgaggtac gcgtgctcga ggtagtcgcg gtagtcgtgg atctcggcgc cggcgtgtcc
     2101 gtcggccgag acgaccgtgt agcggttcgt cgacgacggc tgggcgtttc gcaccggtgc
     2161 tccccctgtg acgcgggcct ggccgtgtaa cgttgttcgt gtaacatcgt tccatgcccg
     2221 gtccggcccg tcaacccggc ctttcccgtg accagatcgt gggtgtggcg ctcgagatcg
     2281 tcgaggccga gggtctggac gggctcacga tgcgccggct cgccgggaag ctcggcgttg
     2341 ccgcgacggc catctattgg cacgtcggtg acaaggaggc gctgctcgac gccctcgccg
     2401 agcggatcgc cgcccgagtc ggcgacatcc cggtcgcggg tgacagcccg gtcgaacgcc
     2461 tcgtgtcgat cggcgcgggt cttcgccgca acctgctcga gcagcccacc ctggtcggcc
     2521 tcgtccacca gcagggtcac accgcgctgc tgttccagcc ggtccgccgg gcgctcgtcc
     2581 gcgagctgac tgccgcggga ttgcgcggcc ccgcgacagc gctcgccttg cacgtgatcc
     2641 tcagtcacat ctcgggctcg gtgctcaacg acgtccagat cgagcgcgcg cccgagcagc
     2701 acgagactcc cgaggagctc tgggcagccg acgaggtgct cgacgatccc gagcttcgcg
     2761 acgcgctggc gcgtccggtc gaccgggacg agctcttcga ctactcgctc accgccctcg
     2821 cgcacgctct catcgatcag tagcgaggtc gccgcggtcg ggtggttcgc gggtcctcga
     2881 aggcagcgct cgcggccgga acgcaccgcc cggcgcaggc cggcagcgtc gtgagggcgc
     2941 ggcaaccgcc gggattcaac tcgccgcagg ggcggagctg gccagggttc cgagctcagc
     3001 catcccgcgc tcgatacctc gacagagcac gtcgaggtcg ggtgcggtgt cgtagtcgcc
     3061 ggtggcgccg aagttcacct gcccgtcgta ggagaagatc gccacggcga tgcggcactg
     3121 ggcgccgagg gggacgtacg gaaaggcggt gagcatgcga cggcccagca cgtagagccg
     3181 atgctggggt cccgggacgt tggtggtcac ggtgttgatg ttgcgttgcg cggccttcgt
     3241 cgcgattcgc gtcccgaggg cgagcaggac cggcggcgcg aatcctgaga gggaggtgag
     3301 ggcctccccg gcgacggcct ggttcgactc cttcagtccg gccagttggg tgctgacggc
     3361 ttgcagtcgt tcggctgggt cggcgatacc gaccggcagc tcggcgaaca tggccgacac
     3421 cttgttgtcg tacgtgccgt ccccgacggc ccggccgctc agatcccggc tgcggaccga
     3481 gacgggcacg agggttcgga tgactcgatc gacggtctcg ccgcgtgagg tcagcaacgc
     3541 gcggaagccc gcggtgatcg cggtgagcac gacatcgttg aaggtgccac cgagctcttt
     3601 gcggatccgc ttgatgtcat cgacggtggt cgaggcccag ccgtagcggc ggtgggggcc
     3661 gatcgggccg ttgaggctcg acgcgggggt tcgggggacg agcccggcga gcgcggcgag
     3721 gccggtgcag atctcgccga gctgcgccgc ggcctggcgg gggacgcggc tggcggctcg
     3781 cagcgcccgc agctgctcgt aggggcttcg agccatggtg gccatcgcgt cggtggcgag
     3841 ggcgagcggg gtaggtgccg gagcggggtg ccacgactcg tcggccgccg ccgcggcgtc
     3901 gggggaggtg tcgagcagga cggtgagcag ctcggtgccg gagacaccgt ccacgatgct
     3961 gtgatgggtc ttgcacagca aggcccagcg gttgttgccc agtccttcca ccatccagag
     4021 ctcccagagc ggcttggtgc ggtccagctg ctggctcatg acgcgcccga cgaggcgacg
     4081 caactcgggt tcgcctccgg gagcgggaag ggcggtgtgg cggacgtggt actcgaggtt
     4141 gaagtcgggg tcgtc
//
LOCUS       DATCCH010000175         2642 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_518027, whole genome shotgun sequence.
ACCESSION   DATCCH010000175 DATCCH010000000
VERSION     DATCCH010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518027"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="VIJ44_03500"
     CDS             complement(<1..491)
                     /locus_tag="VIJ44_03500"
                     /inference="COORDINATES: protein motif:HMM:NF016635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HEY5164998.1"
                     /translation="MATKKIRIYELARELGVENQVVLDLADELKIGVKSHSSSIEDPM
                     ADRVRRLADSRGLRQEEPVEEPEPAPPAPVEAAPSDPAPVAAAPAPADDEPRPVMPHR
                     VVRSTGGIPEPPPLPRVVRSTGGIPEPMPVREPLAPPRPAAAAPRPPAAPPEAPPAPD
                     APQL"
     gene            complement(534..773)
                     /locus_tag="VIJ44_03505"
     CDS             complement(534..773)
                     /locus_tag="VIJ44_03505"
                     /inference="COORDINATES: protein motif:HMM:NF016204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEY5164999.1"
                     /translation="MGCRRATEAANLVRVSLDGAGDLEVGPGPGRGAWLCGPPATIRC
                     LDDAQRRHALDRALRASISAHQLAGLRAKLERLNG"
     gene            complement(796..2094)
                     /gene="nusA"
                     /locus_tag="VIJ44_03510"
     CDS             complement(796..2094)
                     /gene="nusA"
                     /locus_tag="VIJ44_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEY5165000.1"
                     /translation="MNSEMMEALENIEREKGISIEIMLEALANALVTAYKRQPNAAEE
                     ALVEIDIETGDIHVIAQELDEEGQVVREWDDTPKDFGRIAAQTAKQVILQRIRDVERE
                     SKYEEYAGKEGELVTGIIQQTDQRYTLLDLGRVEALLPQAEQVQNERYEHGSRLKAYI
                     VEVRKTAKGPQIVVSRTHPGLVKRLFELEVPEIVDGVVEIRAIAREPGHRTKIAVASN
                     DRNVDPVGACVGARGSRVRMVVNELRGEKVDIVPYTDSPSDFVMKALAPARVREVRIH
                     EDTGTAEVIVPDFQLSLAIGKEGQNARLAARLSGWRVDIKSETQLAEEEAGGGVEYAE
                     GDWVQSDTGELVWQPAEGGDAISAAEAGYASPAAGVATETKPPPPAPPPVAAGSPPAA
                     APKTDPPPEPPAAESAAEPSPSATPASGDAVPEQAANEPA"
     gene            complement(2091..>2642)
                     /locus_tag="VIJ44_03515"
     CDS             complement(2091..>2642)
                     /locus_tag="VIJ44_03515"
                     /inference="COORDINATES: protein motif:HMM:NF036577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEY5165001.1"
                     /translation="RGHVLFVMGAKSAMPDVETIAPAVEAVVVAHGLELYDVELAGPH
                     RSRSLRVLVDRAGGVDLETITGVSEALSRVLDADPTLAQACPGHYTLEVSSPGLERRL
                     RTPDHFRRAVGGTVSIKTRTGDGGLRRRGQLVAADADGVDVDFDTGREHLAYADVAQA
                     RTVFEWGPAPKPGARRNREVATR"
BASE COUNT          308 a          823 c         1050 g          461 t
ORIGIN      
        1 agctgcgggg cgtcgggtgc tggtggcgcc tcgggtggcg cggcaggggg tcggggcgcg
       61 gccgccgccg gacgcggggg cgcgagcggc tcgcgcaccg gcatcggttc ggggatgcca
      121 ccggtcgagc ggacgacacg cggcaagggg ggcggctcgg ggatcccgcc ggtggagcgc
      181 accacccggt ggggcatgac gggtcggggt tcgtcgtcgg ccggggctgg ggccgccgcg
      241 acgggagctg ggtccgaggg tgcggcttca accggtgccg gcggggcggg ctcgggttcc
      301 tcgacgggct cttcctggcg gaggcctctg gaatcggcga gtcggcggac gcggtccgcc
      361 atcgggtcct cgatgctcga cgagtggctc ttgacaccga tcttgagctc atcggcgagg
      421 tcgaggacca cctggttctc gacaccgagc tctcgtgcga gctcgtagat ccggatcttc
      481 ttggtggcca aggcctggtg tcccttccgc ggctggcggc gtctgctggc cggtcagccg
      541 ttcaaacgtt cgagtttcgc gcgcaggcct gcgagctggt gcgccgagat cgatgcccgc
      601 agggcccggt cgagcgcgtg gcgccgttgc gcgtcgtcga ggcagcggat cgtggcaggc
      661 ggcccgcaca gccaggcgcc tcggcccggt cccgggccga cctcgaggtc acccgcgccg
      721 tcgagggaga cccgtacgag gttcgcggcc tcggtggcgc ggcgacatcc cacgcaggtg
      781 cgattcgggc ggcggtcatg cgggctcgtt ggcggcctgc tccgggacag cgtcgccgga
      841 ggcgggggtc gcgctcggtg agggctcggc ggccgactcg gcggcgggcg gttcgggtgg
      901 ggggtcggtc ttcggggcgg ccgcgggggg gctgcccgcc gcaacggggg gtggcgcggg
      961 gggtggcggt ttcgtctcgg tggccacgcc cgcggcgggt gaggcgtatc cggcttcggc
     1021 tgccgagatg gcgtcgccgc cctcggcggg ctgccacacc agctcgccgg tgtcgctttg
     1081 cacccagtcg ccctcggcgt actcgacacc gccgccggct tcttcttctg cgagctgggt
     1141 ctcgctcttg atgtcgactc gccagccgct gaggcgggcg gcgaggcggg cgttctggcc
     1201 ctccttgccg atcgcgagtg agagctggaa gtcggggacg atcacctcgg cggtgccggt
     1261 gtcctcgtgg atgcggactt cgcggacgcg ggccggggcg agcgctttca tcacaaagtc
     1321 ggaggggctg tcggtgtagg ggacgatatc gaccttctcg ccgcgcagtt cgttgacgac
     1381 catgcggacg cgggagccgc gggcaccgac gcaggcaccg acggggtcaa cgttgcggtc
     1441 gttggacgcc accgcgatct tggttcggtg cccgggttcg cgggcgatgg cgcggatctc
     1501 gacgacgccg tcgacgatct cggggacttc gagttcgaag agccgcttga cgagacccgg
     1561 gtgggttcgg gacacgacga tctgggggcc tttggcggtc ttgcggacct cgacgatgta
     1621 ggccttgagc cggctgccgt gttcgtaacg ctcgttctgg acttgctcgg cttggggcag
     1681 gagcgcttcg acgcggccga ggtcgaggag cgtgtagcgc tgatcggtct gttggatgat
     1741 gccagtgacg agctcgccct ctttgccggc gtactcctcg tacttgcttt cgcgttcgac
     1801 gtcacggatg cgttgcaaga tgacttgctt ggcggtctgc gcggcgatgc ggccgaagtc
     1861 tttgggggtg tcatcccatt cgcggacgac ttggccttct tcgtcgagct cttgggcgat
     1921 gacgtgtatg tcgccagttt cgatgtcgat ctcgacgagg gcttcctcag ccgcgttggg
     1981 ttgacgcttg tacgcggtga cgagggcgtt ggcgagcgcc tcgagcatga tctcgatgga
     2041 gatgcccttt tcgcgctcga tgttctcgag cgcttccatc atctcggagt tcatcgggtc
     2101 gccacctccc ggttgcggcg cgcgccgggt ttcggggcgg gaccccactc gaagacggtg
     2161 cgggcttgcg cgacgtcggc gtaggcgagg tgctcacggc cggtgtcgaa gtcgacgtcg
     2221 acgccgtcgg cgtcagcggc gacgagctgg ccccggcggc gcaggccacc atcgccggtg
     2281 cgggtcttga tcgagacggt gccgccgacg gcccgacgga agtggtcggg ggtgcggagg
     2341 cggcgttcga ggccggggct cgagacctcg agggtgtagt ggccgggaca cgcctgggcc
     2401 agggtggggt cggcgtcgag gacccgggac agggcctccg agaccccggt gatggtctcg
     2461 aggtcgacgc cgccagcccg gtcgacgagg acccgcaggc tccgggaccg gtggggtccg
     2521 gcgagctcca cgtcgtaaag ctcgagcccg tgcgcgacga cgacggcttc gaccgcaggg
     2581 gcgatcgtct ccacgtcggg catcgcgctc ttcgctccca tcacaaaaag aacgtggcct
     2641 cg
//
LOCUS       DATCCH010000176         4165 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_51289, whole genome shotgun sequence.
ACCESSION   DATCCH010000176 DATCCH010000000
VERSION     DATCCH010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4165
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51289"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VIJ44_03520"
     CDS             complement(<1..224)
                     /locus_tag="VIJ44_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009156768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165002.1"
                     /translation="MSTETAAGTAGPVHQYAPVVDLHEGATWLDDLRAAKVVWRREII
                     RFRRNRIRILTSLAQPILFLFVLGTGLGTVV"
     gene            complement(221..1240)
                     /locus_tag="VIJ44_03525"
     CDS             complement(221..1240)
                     /locus_tag="VIJ44_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5165003.1"
                     /translation="MAQRGEEELGVAQAATVEVSNLQKRYGALEAVRGVTFDVAPGET
                     FGFLGPNGAGKSTTISMLCTLITPTGGSARVAGFDVVANPLGVRRRIGLVFQDPTLDE
                     YLTAYENLHFHAELYRVPRDRAVERIQAVLEMVGLWERRTSLVRTFSGGMKRRLEIAR
                     GLLHSPRVLFLDEPTVGLDPQTRVHIWDYIQGLRDREDITIFLTTHYMDEAEFCDRIA
                     IIDNGEIVVMDTPDALKAAVGKDRVEVRTEDDDAARQFLGERFSIDATTTSDGLMFHV
                     AAGEEFVPRLFAELDVGIKAVSIARPTLDDVFMRYTGRTIRDAEASSAEKLMINPMIR
                     ARRGR"
     gene            1279..1761
                     /locus_tag="VIJ44_03530"
     CDS             1279..1761
                     /locus_tag="VIJ44_03530"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY5165004.1"
                     /translation="MDNYPRERETAVGPPESPRQLADQLFAVVRRLRQHARELAADHD
                     LSFLQLRALWRLEQPLPTGSLAEQLGLDSSNVTSVVDGLEARGLVARQPLVEDRRVKL
                     LVLTNAGRETRTTIDERMFATLTVFDDLTPAERRQLVTLLAKISPTAPADPTPAPVAP
                     "
     gene            1793..2251
                     /locus_tag="VIJ44_03535"
     CDS             1793..2251
                     /locus_tag="VIJ44_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165005.1"
                     /translation="MALADRRATRCRVLVQDHVPEQVLAAGYVSAAGAANGSTFGGAT
                     TRLIGLVTGSRDGHHAPDAAANAGFAPSSWIGVTATRVYAFAAARGEVGELIGAWDRS
                     AVTVTTSPRLVTTRLSLRFAGAGAGVDLEARRWGAGNRPLLRYLLDPTRR"
     gene            2402..2974
                     /locus_tag="VIJ44_03540"
     CDS             2402..2974
                     /locus_tag="VIJ44_03540"
                     /inference="COORDINATES: protein motif:HMM:NF014639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEY5165006.1"
                     /translation="MHDPTAANTFFTVLTLLANAAVVGLVAVGVGARVSPRGRGLADH
                     VVRAVGPNARGLALLVAATATAGSLYYSEVVGFTPCELCWFQRICMYPLVAVLAVGVL
                     RRDRAAGWYAAPLVAVGAPLGLYHWLVERVPSIASSTSCSLAAPCAVPYFEELGYITL
                     AFMAMSAFLLIAALLIVDRAWARHLPKETS"
     gene            2971..3702
                     /locus_tag="VIJ44_03545"
     CDS             2971..3702
                     /locus_tag="VIJ44_03545"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY5165007.1"
                     /translation="MSPNQRRPAKTGGGNRRAAASRARNWPWWVVGAALVLAGVLAAV
                     LSGGGGSSSSSGGTKPGQVEIASTVAVSGTPLATLSDPTNDPAVGKTAPTLTGVTFNR
                     APVTIGNTGRAHVVVMVAHWCPHCQAEVPRIVSLYKAGQIPVPIVSVATGTDSSAPNY
                     PPSAWLAREGWPYPVLVDSKTETAASSYGLPGYPFLVFVDPQGKVVGRLSGEVAPSDL
                     TKLFAALAAGKPLPIPGAGASSAGK"
     gene            complement(3715..>4165)
                     /locus_tag="VIJ44_03550"
     CDS             complement(3715..>4165)
                     /locus_tag="VIJ44_03550"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY5165008.1"
                     /translation="APGHPGAKARRVVVGTGSMTSRGLASRAGVLAVTAALMLSACGG
                     GGGGGGSGASGSPLPTGAQAHDATLLAGRNVFSAECASCHGARGQGGVGPTFQHERLL
                     RDYFNPEDQVTFVSNGKGIMPSFAGVLTHTQLEAVVAYTRQVLTHAK"
BASE COUNT          570 a         1484 c         1482 g          629 t
ORIGIN      
        1 accaccgtgc cgagcccggt gccgaggacg aacaggaaca ggatcggttg ggcgagcgac
       61 gtcaggatcc ggatgcggtt ccgtcggaag cggatgatct cccggcgcca cacgaccttc
      121 gccgctcgga ggtcgtcgag ccaggtggca ccctcgtgga ggtcgaccac gggggcgtac
      181 tgatggacgg ggccggcggt gcccgccgcg gtctcggtgc tcatcggcct cgccgggccc
      241 ggatcatcgg gttgatcatg agcttctccg cgctcgacgc ctcggcgtcg cggatggtgc
      301 gcccggtgta gcgcatgaag acgtcgtcga gggtcgggcg ggcgatcgag accgccttga
      361 tgccgacgtc gagctccgcg aacaggcgcg ggacgaactc ctcgccggcc gcgacgtgga
      421 acatcagccc gtcggacgtg gtggtggcgt cgatgctgaa gcgctccccg aggaactggc
      481 gggccgcgtc gtcgtcctcg gtgcggacct cgacgcggtc tttgcccacg gcggccttga
      541 gcgcgtcggg ggtgtccatg acgacgatct cgccgttgtc gatgatcgcg atgcggtcac
      601 agaactcggc ttcgtccatg tagtgggtgg taaggaagat cgtgatgtct tcacggtccc
      661 gcagcccctg gatgtagtcc cagatgtgga cccgggtctg ggggtcgagg cccaccgtcg
      721 gctcgtcgag gaacaagacg cgcggcgagt gcaggaggcc gcgggcgatc tcgaggcgtc
      781 gcttcatgcc gccggagaag gtgcggacga ggctggtgcg tcgctcccac aggccgacca
      841 tctcgaggac ggcctggatc cgctcgacgg cgcggtcccg ggggacgcgg tacagctcgg
      901 cgtggaagtg caggttctcg taggcggtca ggtactcgtc gagggtcggg tcctggaaca
      961 ccaggccgat gcggcgacgg acgccgagcg ggttcgcgac gacgtcaaag cccgccaccc
     1021 gggccgaccc accggtcggg gtgatcaggg tgcagagcat cgagatggtg gtggacttcc
     1081 ccgccccgtt cgggccgagg aagccgaagg tctcgcccgg cgcgacgtcg aacgtcaccc
     1141 cgcggaccgc ctcgagggcg ccgtagcgct tctggaggtt cgagacctcg accgtcgcgg
     1201 cctgggccac cccgagctcc tcctctccgc gctgcgccat cgatgctgac cgaatgtcgg
     1261 tactctaccc ttacggtgat ggataactat ccgcgggaga gggaaacggc ggtggggcca
     1321 cccgaatcgc ctcgccagct cgccgaccag ttgttcgcgg tcgttcgacg gctccgccag
     1381 cacgcgaggg agttggccgc tgaccacgac ttgtcgttcc tccagctgcg ggccttgtgg
     1441 cgcctcgagc agccgctccc gacggggtcg ctcgccgagc agctcggcct cgactcctcg
     1501 aacgtgacca gcgtcgtgga cggcctcgag gcacgcggcc tcgtggcccg ccagcccctg
     1561 gtcgaggacc gtcgggtcaa gctgctcgtc ctcaccaacg ccggccggga gacccgaacc
     1621 acgatcgacg agcggatgtt cgcgacactg accgtcttcg acgacctcac ccccgccgaa
     1681 cgtcgtcagc tcgtgacgct cttggccaag atcagcccga cggctcccgc cgaccccaca
     1741 ccggcgcccg tcgccccgta acccgaccga aacgaagtcg gaggttttgc tcgtggccct
     1801 cgcggaccgc cgcgccaccc ggtgtcgagt cctcgttcaa gaccacgtcc cggagcaggt
     1861 gctcgccgcc gggtatgtct ccgccgccgg cgcggcgaac gggtcgacgt tcgggggagc
     1921 gaccacgcgt ctgatcgggc tcgtcaccgg ttcgcgcgac gggcaccacg cgcccgacgc
     1981 ggcggcgaac gccgggtttg cacccagctc gtggatcggc gtcaccgcga cccgcgtcta
     2041 cgcgttcgcg gccgcacgcg gcgaggtcgg tgagctcatc ggcgcgtggg accggagcgc
     2101 ggtgacggtg accacgtcgc ctcgattggt gacgacccgc ctctcgctgc gcttcgcggg
     2161 cgcgggcgcc ggcgtggatc tcgaggcccg aaggtggggg gccgggaacc gtccgctgct
     2221 gcgctacctc ctcgatccga cgcgccggtg atgagccggg gtctcagctt cgtccgatcg
     2281 ggcccgcgtc cacgattcgc gcttcggcag cgatactgga ccgatgggcc ggcttcgcac
     2341 cgttgatggg ccgcaccgat gacgcacggg tcgtcggccc ggcggggccg gtgaggctca
     2401 cgtgcacgac ccgacggccg cgaacacctt cttcaccgtc ctcaccctcc tcgccaacgc
     2461 tgcggtggtc ggcctcgtgg ccgtcggggt cggcgcgcgg gtctcgcccc gggggcgggg
     2521 cctcgccgac cacgtggtcc gcgccgtcgg cccgaacgcc cggggactgg cgctgctcgt
     2581 cgcggcgacg gccaccgcgg gcagcttgta ctactccgag gtcgtcggct tcacaccgtg
     2641 cgagctgtgc tggttccagc gcatctgcat gtaccccctg gtggctgtcc tcgccgtcgg
     2701 ggtcctccgg cgcgaccgtg ccgcggggtg gtatgccgca ccgctggtcg cggtcggcgc
     2761 accgctcggg ctctaccact ggctggtcga gcgggtcccg tcgatcgcct cgtcgacgtc
     2821 gtgttccctc gccgcgccgt gcgccgtccc ctacttcgag gaactcgggt acatcacgtt
     2881 ggcgttcatg gcgatgagcg cgttcttgct catcgcagcg ctgctgatcg tcgaccgggc
     2941 ctgggcccgc caccttccga aggagacctc gtgagcccca accagcgccg tccggcgaag
     3001 accggtggtg ggaaccgacg cgccgcggcg tcgcgtgctc ggaactggcc ctggtgggtc
     3061 gtcggggcgg ccctcgtgct cgccggtgtc ctcgcggccg tgctgagcgg cggtggaggc
     3121 agcagcagct cgagcggcgg aaccaagccg ggccaggtcg agatcgcgtc cacggtcgcg
     3181 gtcagcggca cgccgctggc gaccttgtcc gatcccacca acgacccggc ggtcggcaag
     3241 accgccccca ccctgaccgg agtgacgttc aatcgggccc cggtgacgat cggcaacacc
     3301 ggtcgggccc acgtggtcgt catggtggcg cactggtgcc cgcactgcca ggcggaggtg
     3361 ccccgcatcg tctcgctcta caaggcggga cagatcccgg tcccgatcgt gagcgtcgcg
     3421 acgggaacgg actcgtcggc gcccaactac ccgccgtcgg catggctggc ccgcgagggg
     3481 tggccgtacc cggtgctcgt ggactccaag accgagaccg cggccagcag ctacgggctg
     3541 ccgggctacc cgttcctggt gttcgtcgac ccgcaaggca aggtggtcgg ccggctcagc
     3601 ggggaagtgg cccccagtga cctgacgaag ctgttcgcgg cgctcgccgc ggggaagccg
     3661 ctcccgatcc ccggcgcggg ggcatcgtcg gccggcaaat agccaccggc cgcgttactt
     3721 ggcgtgcgtc agcacctgcc gcgtgtaggc gaccaccgcc tcgagctgag tgtgggtcag
     3781 gacccccgcg aacgacggca tgatcccctt gccgttcgac acgaacgtca cctggtcctc
     3841 ggggttgaag tagtcgcgca gcagcctctc gtgctgaaac gtcgggccaa cccccccttg
     3901 gccgcgagcg ccatgacacg aggcgcactc ggccgagaag acgttgcgcc ccgcgagcag
     3961 cgtggcgtca tgagcttgcg cccccgtcgg gagcggagag cccgacgcgc cggacccacc
     4021 accaccgccg ccgccgccgc aggcgctcag catcagcgcg gcggtgacgg cgagcacgcc
     4081 agcccgggag gcaagcccgc ggctcgtcat cgacccggtc cccaccacga cccggcgcgc
     4141 cttcgctccg gggtgtcccg gggcg
//
LOCUS       DATCCH010000177         2217 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_476876, whole genome shotgun sequence.
ACCESSION   DATCCH010000177 DATCCH010000000
VERSION     DATCCH010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476876"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VIJ44_03555"
     CDS             <1..392
                     /locus_tag="VIJ44_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HEY5165009.1"
                     /translation="EDVPYGKLALLARGLANVERYDNGRLTLTALTATDFSDSDAEES
                     YSEGVVDHLRAVQGTEVAALVRDRIGDGVAGLRKVSLRSGNDRIDVSAIARAQGGGGH
                     RQAAGFTTGLSHDELVAFLRGALADQS"
     gene            437..1294
                     /gene="truB"
                     /locus_tag="VIJ44_03560"
     CDS             437..1294
                     /gene="truB"
                     /locus_tag="VIJ44_03560"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEY5165010.1"
                     /translation="MPGDDRPRDYVLLYDKPAGVTSHDVVATVRRGLARTTKVGHAGT
                     LDPFATGLLLVLVGRATRVQRFLMALPKRYETIARFGAVSSTGDPDGDITLTGRLPAG
                     DLELPVGVIRQRPPAYSAVKVGGRRAYELARAGESVELAEREVTVYRFEQSWQEADRR
                     AFVIECSSGTYVRTLISELGDAYCEALRRTRIGRFDVDDADPERPIAVSDALDFMPEV
                     RLDADAARRAAFGQAVGGGAQDLAAVAGGGPDAAPAPTVRLTDAHGLVALAQPTEDGT
                     ALRAVVGFR"
     gene            1341..2207
                     /locus_tag="VIJ44_03565"
     CDS             1341..2207
                     /locus_tag="VIJ44_03565"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEY5165011.1"
                     /translation="MQVTMLADTQPRPRRVAVGEFDGVHLGHREVIAGNDTVLTFDLH
                     PLAVIRPEAAPKLLTSLEVKIELVAALGVQELVVIPFDHRFGAQSPQEFIDHVLVDQL
                     QATHVSVGENFYFGHGAAGDTDMLAADPRFETRVVTLVEVDGEIVSSSHIRGLVLAGE
                     VEQAARFLGAPFALRGDVVHGDKRGRTLGFPTANLVPDEHLVCPGHGVYAARAAGACA
                     AVSIGVRPTFGSGRSLLVEAYLLDRDEDLYGCVLRLDFLRRLRGEQRFDSAEELIAQM
                     KIDVAQTREVCG"
BASE COUNT          301 a          803 c          791 g          322 t
ORIGIN      
        1 acgaggacgt gccctatggc aagcttgcac tgctggcgcg gggcctggcc aacgtcgagc
       61 gctacgacaa cggacggctg acgctgaccg cgctgacggc caccgacttc tcggactcag
      121 acgccgagga gagctactcc gagggcgtcg tcgaccatct gcgggccgtg cagggaaccg
      181 aggtggcggc gctcgtccgt gaccgcatcg gggatggcgt ggccggcctg cgcaaggtct
      241 cgctacgctc aggcaacgac cgcatcgacg tctcggctat cgcccgcgcc cagggtggcg
      301 gcggccaccg ccaggcggcc ggcttcacca ccgggctgtc gcacgacgag ctcgtggcct
      361 tcctgcgcgg cgccctcgcc gaccagtcct agcctagact agctttccgc tccacccggg
      421 cgatagcttc ggagacatgc ccggtgacga ccgtccgcgc gactacgtcc tgctgtacga
      481 caaaccggcg ggggtgacct cgcacgatgt ggtcgcgacg gtgcgccgcg ggttagcgcg
      541 cacaaccaag gtcggccacg ccggcacgct ggacccgttc gccaccgggc tgctgctcgt
      601 gctcgtcggc cgagccaccc gcgtgcagcg cttcctgatg gcgctgccca agcgctacga
      661 gacgatcgcc cgcttcgggg cggtctcgag caccggggat cccgacggag acatcacctt
      721 gaccggccgg ctgcccgccg gggacctgga gctgcccgtc ggggtcatcc gccagcggcc
      781 gccggcctac tcggccgtca aggtcggtgg gcggcgcgcg tacgagctgg cccgggcggg
      841 ggagagcgtg gagctggccg agcgcgaggt caccgtgtac cgcttcgagc agtcctggca
      901 agaggccgac cggcgcgcgt tcgtgatcga gtgctcgtcg ggcacctatg tacgcacgct
      961 gatctccgag ctgggcgacg cctactgcga ggcgctgcgg cgcacccgga tcggccgctt
     1021 cgacgttgac gacgccgacc ccgagcggcc gatcgccgtc tcggacgccc tggacttcat
     1081 gccggaggtg cgtctggacg cggacgcggc ccgccgggcg gccttcggcc aggctgtcgg
     1141 gggaggcgca caggacctcg cggccgtcgc ggggggcgga cccgacgccg cccccgcgcc
     1201 gacggtgagg ctgactgacg cccacggtct ggtggccctc gcccaaccga ccgaggacgg
     1261 caccgcattg cgggcggtcg ttggattccg ctaggccccg ggaacgggta ccggcgcgcc
     1321 gccgaatacg cttgtccgcg atgcaggtca cgatgctcgc cgacacgcag ccgcgcccgc
     1381 gacgggtggc ggtgggggag ttcgacgggg ttcacctcgg ccaccgcgag gtgatcgccg
     1441 gcaacgacac cgtgctcacg ttcgacctcc acccgctggc cgtgatccgg cccgaggcgg
     1501 cgcccaagct cttgaccagc ctcgaggtca agatcgagct cgtcgccgcg ctgggcgtcc
     1561 aggagctggt cgtgatcccc ttcgaccacc gcttcggcgc ccagagcccg caggagttca
     1621 tcgatcacgt gctcgtcgat cagctgcagg ccacgcacgt ctcggtcggc gagaacttct
     1681 acttcgggca tggcgccgcc ggggacaccg acatgctggc cgccgacccg cgctttgaga
     1741 cccgagtggt gacgctcgtc gaggtggacg gcgagatcgt ctcctccagc cacatccgcg
     1801 ggctcgtgct cgccggcgaa gtcgagcagg cggcgcggtt tctcggcgcc ccattcgcgc
     1861 tgcggggcga cgtcgtgcac ggggacaagc gcgggcgcac gctcggcttt cccacggcca
     1921 acctcgtccc cgacgagcac ctggtgtgtc ccggccatgg ggtgtacgcc gcccgcgccg
     1981 ccggggcctg cgcggcggtc agcatcggcg tgcgcccgac cttcggctcg gggcgttcac
     2041 tgctggtcga ggcctatctg ctcgaccgcg acgaggacct ctacggttgc gtcctccggc
     2101 tcgactttct ccgccggctg cgcggcgagc agcgcttcga cagcgccgag gagctcatcg
     2161 cccagatgaa gatcgacgtg gcccagaccc gcgaggtgtg cggttgaccg gcgccgg
//
LOCUS       DATCCH010000178         2870 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_186316, whole genome shotgun sequence.
ACCESSION   DATCCH010000178 DATCCH010000000
VERSION     DATCCH010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186316"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1455
                     /locus_tag="VIJ44_03570"
     CDS             <1..1455
                     /locus_tag="VIJ44_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013424466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA synthetase"
                     /protein_id="HEY5165012.1"
                     /translation="DDGAPEPLAMWDDVVQRAADDSSAGRRALDRLDSIDVVFCQTWQ
                     YDDPPGRLAERLGVDPRRQEYSGIGGTTPQVLVQNQAARILRGESDLGLVCGAEALAT
                     QRCYKRRSERYPYSFKPAEKRPYPWEAPFHPAELAHDVMQAWLTFAVFDNARRAHRGE
                     ALADYRHEMGEMLAPMTRVAAKNPHAWFPIARTPDEIIDPRPENRLVGYPYTKYMISI
                     MDVDMASAVLVASHETADALGVPADQRVYLRGWCYATDATYVAEHDTLWGSPAMAAAS
                     RVALERAGIGVDDAAHLDLYSCFASSLNFAKDALGIGDDDDRALTVTGGLPYHGGAGS
                     NYMTYSIAAMTDVLRADPGSYGLVSGVGMHMTKHVYGVYSAMPGPVSPPDQDRVQREL
                     DAAHPPRSIVDSYDGVATVAAYSVIHDRDGNPASGVLVCDVDGARTYAKVLDPATLTS
                     AEEREMVGATVRLSPQPTPLPMGGEGTANLAVPA"
     gene            1473..2774
                     /locus_tag="VIJ44_03575"
     CDS             1473..2774
                     /locus_tag="VIJ44_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY5165013.1"
                     /translation="MAWDFSTEPEFQAKLDWIREFVENEIEPLDLAFSSHAVYDRSHP
                     VHREVVRPLQAQVKAHDLWACHLGPDLGGKGYGQLNLALMNEILGRSQWAPSVFGCQA
                     PDSGNAEIIAHYGTEEQKAKYLQPLLDGEIVSCYSMTEPQAGSDPGQFKCGATKDGGE
                     WVIEGEKWFSSNLRYADFAIVMAVTDPDVSVYQGTSMFLVPTSTPGVDIIRNTGLGSE
                     PEGEGSHAYVHYNQVRVPAENLLGGEGQAFVIAQTRLGGGRVHHAMRTIGMCQRAFDM
                     MCRRALSRVTQGEVLAKKQMVQEAVADSYIDLEQFRLLVLQTAWKIDQVGGHKARTEI
                     AAVKVLTPKVLHDIVYRALHIHGALGVSNEMPLSGMWAMLPVMGIADGPTEVHKVTIA
                     RQVLKRYQPYEGLWPPEHLPERRAAARKKFVHLLEHQAAEL"
BASE COUNT          487 a         1025 c          941 g          417 t
ORIGIN      
        1 gacgacggcg cacccgaacc gttggccatg tgggacgacg ttgttcagcg ggccgcggac
       61 gacagcagcg cggggaggcg ggccctcgac cggctggact ccatcgacgt ggtgttctgt
      121 cagacctggc agtacgacga cccgccgggc cggctcgccg aacggctcgg cgtcgaccct
      181 cgacgccagg agtactccgg gatcggcggc accacccccc aggtcctggt ccagaaccag
      241 gcggcccgga tcctgcgcgg cgagagcgac ctgggcctgg tctgcggcgc ggaggcgctc
      301 gccacccaac ggtgctacaa gcgtcgtagc gagcggtacc cgtactcgtt caagcccgcc
      361 gagaagcgcc cctacccgtg ggaggcgccg ttccatcccg ccgagctcgc ccatgacgtg
      421 atgcaggcct ggctcacgtt cgcggtgttc gacaacgccc gccgcgcaca ccgcggcgag
      481 gcgctcgccg actaccggca cgagatgggc gagatgctcg cccccatgac ccgcgtcgcg
      541 gccaagaacc cgcacgcctg gttcccgatc gcccgcaccc ccgacgagat catcgatccc
      601 cggcccgaga accgcctcgt cggctatccc tacaccaagt acatgatctc gatcatggac
      661 gtcgacatgg ccagcgccgt cctcgtggcg agccacgaga ccgccgatgc actcggcgtc
      721 cccgcggacc agcgggtcta cctccgcggc tggtgctacg ccaccgacgc cacctacgtc
      781 gcggagcacg acaccctgtg ggggtcgccg gccatggccg ccgcgtctcg agtcgccctg
      841 gagcgcgccg ggatcggcgt cgacgacgcc gcgcacctcg atctgtactc ctgcttcgcc
      901 agctcgctga acttcgccaa ggacgcgctc ggcatcggtg acgacgatga ccgggccctg
      961 accgtcaccg gcgggctgcc gtaccacggc ggtgccggca gcaactacat gacctactcg
     1021 atcgccgcca tgaccgacgt cctgcgcgcc gaccccggct cgtacggcct ggtgagcggg
     1081 gtcgggatgc acatgacgaa gcacgtctac ggcgtgtaca gcgccatgcc gggtccagtg
     1141 tcgccgcccg atcaggaccg ggtgcagcgc gagctcgacg cggcgcaccc gcctcggtcg
     1201 atcgtcgact cctatgacgg cgtcgcgacc gtcgccgcgt actcggtgat ccacgaccga
     1261 gacggcaacc cggcgtcggg ggtcctcgtg tgcgatgtgg acggcgctcg cacctacgcc
     1321 aaggtgctcg acccggccac gctgacctcg gccgaagaac gtgagatggt gggcgcgacg
     1381 gtgcggctct cgccgcagcc gacaccgctg cccatgggtg gagagggcac cgccaacctg
     1441 gcggtgccgg catgagagtg aggtgacgtc cgatggcatg ggacttctcg actgagccgg
     1501 agttccaagc gaagctggac tggatccgcg agttcgtgga gaacgagatc gagccgctcg
     1561 acctcgcgtt cagctcgcac gccgtctatg accgatccca tccggtgcac cgcgaggtgg
     1621 tgcggcccct ccaggcccag gtcaaggccc acgacctctg ggcgtgtcac ctcggcccgg
     1681 acctcggcgg gaagggctac gggcagctga acctggcgct gatgaacgag attctcgggc
     1741 ggtcacaatg ggcgcccagc gtgttcggat gccaggcgcc cgactccggc aacgccgaga
     1801 tcatcgccca ctacggcacc gaggagcaga aggcgaagta cctccagccg ctcctcgacg
     1861 gcgagatcgt ctcgtgctac tcgatgaccg aaccccaggc gggatccgac ccgggccagt
     1921 tcaagtgcgg agccaccaaa gacgggggcg agtgggtcat cgagggcgag aagtggttct
     1981 cgtcgaacct ccgctacgcc gacttcgcca tcgtcatggc cgtcaccgac cccgatgtgt
     2041 cggtgtacca gggcacgtcg atgttcctcg tcccgacctc cacgcccggg gtcgacatca
     2101 tccgcaacac cgggctcggc agcgaacccg aaggtgaggg ctcacacgcc tacgtccact
     2161 acaaccaggt gcgcgtcccc gctgagaacc tcctcggcgg agaaggtcaa gccttcgtca
     2221 tcgcacagac ccgcctcggt ggtggccgtg ttcatcacgc catgcgcacc atcggcatgt
     2281 gccagcgggc cttcgacatg atgtgtcgac gggcactgtc acgcgtgacc caaggcgagg
     2341 tgctcgcgaa gaagcagatg gtccaagagg cggtcgctga ctcgtacatc gatctcgagc
     2401 agttccgact cctcgtcttg cagaccgcgt ggaagatcga ccaggtcggt ggccacaagg
     2461 cccgcaccga gatcgccgcg gtgaaggtcc tcaccccgaa ggtgctccac gacatcgtgt
     2521 accgggcgtt gcacatccac ggcgcgctgg gagtctcgaa cgagatgccg ctctcgggga
     2581 tgtgggcgat gctgccggtg atggggatcg ccgacgggcc gaccgaggtc cacaaggtca
     2641 cgatcgcccg tcaggtcctc aagcgctatc agccctacga ggggctctgg ccccccgagc
     2701 atctccccga gcggcgcgcc gcggcccgca agaagttcgt gcatctcctc gagcatcagg
     2761 ccgccgagct gtgaccgaca tgtccaacgt ctgggagacc gccgacgacg ccgcccgcac
     2821 ggagggtctc gtcgacacag acacgctcac cgcgtggatg gatgcccgcg
//
LOCUS       DATCCH010000179         2561 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_269320, whole genome shotgun sequence.
ACCESSION   DATCCH010000179 DATCCH010000000
VERSION     DATCCH010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269320"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="VIJ44_03580"
     CDS             complement(<1..851)
                     /locus_tag="VIJ44_03580"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HEY5165014.1"
                     /translation="MDLEQFCSASRIVVVAGKGGVGKTTVTAALATAAARAGASVLIV
                     EVEGKSGLAATLGCPPLTYEEQPVRERLRARTLTPDEALREYLEDRGLHRISRRLMKT
                     GALDVVATAVPGMKDILVLGKVKALEQANDADLILIDTPAAGHAINFLLSPRGLLDAV
                     RVGPVRAQAEDVIELLSDPARCRVALVTLAEETPVNELVDTAFALEDRVGVKLAPVIV
                     NAVPDDLPVDGHSAAADAAVLGITLGPGEAEALDAAATFRRERHALALEQADRLAGRL
                     PLPQLHLP"
     gene            937..2358
                     /locus_tag="VIJ44_03585"
     CDS             937..2358
                     /locus_tag="VIJ44_03585"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEY5165015.1"
                     /translation="MGCVLVVFALASVAVGASSATSPSAPPPHGRRGITVVQVEGYID
                     PPVATLIRDAIRSANQSGSTLLLMQLDSSGALDVDIASLVRQIRDSRVPIAVWVGPSG
                     AHAKGGAALLAEAAPILFVAPGSDLGPGVPVRLDHPAGSVGSVQAQLGAQAVANGRDQ
                     PAARSLAGRSLGATDAGRLGVTNGVRPTVGEVIVTLDGRTVRTGAGPIRLSTARVIGT
                     GRGRRRQPNQQVVFDSLGLGARVQHTFISPQVAYLLLVVGLSLIVFEFFAASVGFAAA
                     VGALTVVGATYGFSHLPVAWWAAALLAGAVTAFAIDVQAGGVGAWTVIGTAALIAGSF
                     TLYGGSSRLRVPWWELVLVVAGTLLFFVGALPAFVRSRFSTPTIGREALVGEVGDAEV
                     AVDPDGVVMIRGSRWRARTNRATPIPAGAAVRVVAVEGLVLEVEPEEGGAKDYRDRAR
                     SRGRAGGLDPGAEPAAGDDTTFS"
BASE COUNT          314 a          890 c          968 g          389 t
ORIGIN      
        1 ggcagatgga gctgtggaag cgggaggcga ccggcgagcc ggtcggcctg ctcgagcgcg
       61 agcgcgtgac gctcacgacg aaacgtggcg gcggcatcga gcgcttcggc ttcccccggg
      121 ccgagggtga tgcccaagac cgcggcgtcg gccgcggccg agtggccgtc gacgggcagg
      181 tcgtcgggga ccgcgttgac gatgaccggt gcgagcttca ccccgacgcg gtcctcgagc
      241 gcgaaggccg tgtcgaccag ctcgttcacc ggggtctcct cggcgagggt cacgagcgcg
      301 acccgacatc gggccgggtc ggagagcagc tcgatgacat cctcggcttg ggcccggacc
      361 ggccccaccc ggaccgcgtc gagcaggccc cggggcgaga ggaggaagtt gatggcgtgc
      421 cccgcggcgg gagtgtcgat gaggatgaga tccgcgtcgt tggcttgttc gagcgccttg
      481 accttcccga gcaccaagat gtccttcatg cccggtaccg cggtggcgac gacgtcgaga
      541 gcccctgttt tcataagtcg ccgagagatc cgatgcaggc cccggtcctc gaggtactcc
      601 cggagcgcct cgtcgggcgt gagggtccgg gcccgcagtc gttcgcgaac cggttgctcc
      661 tcgtaggtga gcggggggca gccgagcgtg gcggcgaggc ccgatttgcc ttcgacctcg
      721 acgatcagca cgctggcccc ggcgcgggcg gcggcggtcg cgagcgcggc ggtgacggtc
      781 gtcttgccga cgcctccttt gcccgccacg acgacgatgc gagaggcgga gcagaactgc
      841 tccaggtcca tggttgactc cgcaaggccg cgctcggtgc tggttcgctc gcatgctagg
      901 aggggagcac cgaggatccg gttccgccac gtcacggtgg gctgtgtcct cgtggtgttc
      961 gccctcgcca gcgtcgccgt cggtgcctcg tccgcgacat cgccgtcggc gccgccaccg
     1021 catggccggc gaggcatcac cgtggtgcag gtcgagggct acatcgatcc gccggtcgcg
     1081 acgctcatcc gagacgcgat ccgttcggcg aaccagtccg gttcgacgct cctgctgatg
     1141 cagctcgact ccagcggagc cctcgatgtc gacatcgcct cgctggtgcg ccagatccgc
     1201 gactcgcggg tgccgatcgc ggtctgggtc ggaccgtccg gtgcccacgc caagggcggc
     1261 gccgcgctgc tcgccgaggc cgcgccgatc ctgttcgtcg ctcccggctc ggacctgggt
     1321 ccgggtgtcc ccgtccgcct cgaccacccg gccggctccg tgggctcggt ccaagctcag
     1381 ctcggggccc aggccgtcgc gaacggtcgg gaccagccgg ccgcgcgcag cctcgccgga
     1441 cggagcctcg gggcgaccga cgcgggacgc ctcggcgtga ccaatggcgt gcgaccgacg
     1501 gtcggtgagg tgatcgtcac cctcgatggg cggaccgtgc ggaccggcgc gggtccgatc
     1561 cggctctcga cggcccgggt gatcgggacg ggacgaggcc ggcgacgcca gcccaaccag
     1621 caggtggtgt tcgacagtct cgggctcggc gctcgggtcc agcacacgtt catcagtccg
     1681 caggtcgcct acctgctcct ggtcgtgggg ctgtcgttga tcgtcttcga gttcttcgcc
     1741 gcgagcgtcg gcttcgccgc cgcggtcggg gcgctcaccg tcgtcggcgc cacgtacggc
     1801 ttctcgcacc tcccggtggc gtggtgggcc gcggcgctgc tcgcgggggc ggtgacggcc
     1861 ttcgcgatcg atgtgcaggc aggcggcgtc ggcgcctgga cggtcatcgg caccgcggcg
     1921 ctgatcgccg gctcgttcac cctctacggc ggctcgagcc gcctccgggt gccgtggtgg
     1981 gagctcgtgc tcgtcgtcgc cggcaccctc ttgttcttcg tcggggcgct cccggccttc
     2041 gtgcggtccc ggttctcgac cccgacgatc gggcgcgagg cgctcgtcgg ggaggtcggc
     2101 gacgccgagg tcgccgtgga tcccgacggc gtcgtgatga tccggggcag ccggtggcgc
     2161 gcccgcacca accgggccac gccgatccca gctggagccg cggttcgggt ggtggccgtc
     2221 gaaggcctcg tcttggaggt cgaacccgag gagggcggag cgaaggacta ccgcgaccga
     2281 gcccgttccc gaggacgggc cggggggctc gatcccggcg ccgagccggc cgccggggac
     2341 gacaccacct tttcttgacc ccggggacgg gggtaacgtc cgcgcgtcgg cccggatcgt
     2401 tgcgggtgac gagaggggga atcgtggcgc ttgaccgcgt cgccgaggct tggcagcgcc
     2461 aggccgcctg tcgaggcccg gaatcggcgc tcttctaccc gccagccacg cccgaacctc
     2521 ggccggagcg ggacagccgg gagcggcggg cgaaggcgat c
//
LOCUS       DATCCH010000180         2819 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_477056, whole genome shotgun sequence.
ACCESSION   DATCCH010000180 DATCCH010000000
VERSION     DATCCH010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477056"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /gene="rplJ"
                     /locus_tag="VIJ44_03590"
     CDS             complement(<1..596)
                     /gene="rplJ"
                     /locus_tag="VIJ44_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014378016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEY5165016.1"
                     /translation="MARPDKVAVVEEVRDKLRDADAAVLTEYRGLSVPELAQLRGTLR
                     PSSTEYKVFKNSLARRAAEAAGLADLLPYLEGPVAIAFVHGDAVIAAKALRDFGRTNP
                     ALVIKGGLLGPRVLSGAEVETLAEIEPRDVLLARLAGGFQAPLTKAAGLFQAFTRNLA
                     YGVQAYIDQRVAAGEPAPAPTAPAADGPDTTTPETAGA"
     gene            complement(767..1492)
                     /gene="rplA"
                     /locus_tag="VIJ44_03595"
     CDS             complement(767..1492)
                     /gene="rplA"
                     /locus_tag="VIJ44_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009278400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEY5165017.1"
                     /translation="MTKHGKAYADATQRYERDRAMTARDALELVRSLAPAKFDETVEA
                     AFRLGVDPRKADQMVRGTVSLPNGTGKFVRVAVFAAGDAAREAEAAGADIVGTDDLVS
                     RVEAGDLDFDVAIATPDLMGKVGKLGRTLGPRGLMPNPKTGTVTTDVGKAVQEFKAGK
                     VEYRTDRNGNVHVPIGKVSFDTNALLDNYHAVLDELTRAKPAAAKGRYIKAVSAASTM
                     GPGVKIDPALGREDDSPQATPPG"
     gene            complement(1572..2000)
                     /gene="rplK"
                     /locus_tag="VIJ44_03600"
     CDS             complement(1572..2000)
                     /gene="rplK"
                     /locus_tag="VIJ44_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEY5165018.1"
                     /translation="MAKKRLAAVVKIQIQAGQATPAPPVGTALGPHGINIMDFCKAYN
                     AATESRRGQVIPAEISVFEDRSFTFVTKTPPTPFLLRQAAGIEKGAQNPRLEKVGQVT
                     KTQVRKIAETKMPDLNAVDLDGAVAQVAGTARSMGIQVVE"
     gene            complement(2034..2564)
                     /gene="nusG"
                     /locus_tag="VIJ44_03605"
     CDS             complement(2034..2564)
                     /gene="nusG"
                     /locus_tag="VIJ44_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017473683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEY5165019.1"
                     /translation="MHTYAGYENKVKQNLEQRIRSQHAEDQIFEVVIPMEDVIEFKGG
                     QRRVVPKKVFPGYVLVRMVMNDGSWRVVRDTPNVTGFVGPSGRGEKPTPLSRKEVEDI
                     LGVAKPDSPAAEKKVRPRVEFEEGEQVRVTTGPFADFNGAISEIDVDRSKLKVLVNIF
                     GRETPVELEFGQVAKL"
BASE COUNT          378 a          870 c         1049 g          522 t
ORIGIN      
        1 tcggcgccgg cggtctcagg ggtggtggtg tcgggaccgt cggcggcggg ggccgtcggg
       61 gcgggggcgg gttcgccggc ggcgacgcgc tggtcgatgt aggcctgcac gccgtaggcg
      121 aggttgcggg tgaacgcctg aaacaggcca gcggctttcg tgagcggcgc ttggaagcct
      181 ccggcgagcc gggcaaggag gacgtcgcgg ggttcgatct cggcgagggt ctcgacctcg
      241 gcgccgctga ggacccgagg gccgagcagg cctcccttga tgacgagagc cgggttggtg
      301 cgcccgaagt ctcggagggc tttggccgcg atgaccgcat cgccgtggac gaacgcgatc
      361 gcgacgggtc cctcgaggta agggagcaga tcggcgagac cggcagcctc ggcggcccgg
      421 cgggccaggc tgttcttgaa gaccttgtac tcggtgctgg aggggcgcag cgtccctcgc
      481 agttgggcca actcggggac gctgaggcct cggtactcgg tgaggacggc ggcgtcggcg
      541 tcgcgcagct tgtcgcgcac ctcctcgacg accgccacct tgtccggcct tgccatcact
      601 gctccctcga cgacgaaacg gagtgccgcg gatgctcccg gaacaggagc acgagacacc
      661 ccgtgcgagc gaggtcgcct cgttcggcgg gacgattccc attacctcct cgccgaggcg
      721 gggagaccga aggtctgcgg caacgacttc agttgtgggc ggaaggctac ccgggcggag
      781 tggcctgggg cgagtcgtcc tcgcgtccga gagccgggtc gatcttgacg ccgggaccca
      841 tggtcgacgc cgcggacacc gccttgatgt agcgaccttt cgcggcggcc ggtttggctc
      901 gggtgagctc gtcgagcacc gcgtggtagt tgtcgagcag cgcgttggtg tcgaagctca
      961 ccttgccgat ggggacgtgg acattgccgt tccggtcggt gcggtactcg accttgcccg
     1021 ccttgaactc ctggacggct ttaccaacgt cggtggtgac cgtgccggtc ttggggttcg
     1081 gcatgagccc gcggggtccg agcgtccgac cgagcttgcc gacctttccc atgaggtcgg
     1141 gggtggcgat cgcgacgtcg aagtcgagat cacctgcttc gacgcgagat accaggtcgt
     1201 cggttccgac gatgtcggcc ccggcggctt cggcctctcg ggcggcgtcg ccggcggcga
     1261 agacagcgac ccggacgaat tttccggtgc cgttcggaag cgagacggtg cctcgcacca
     1321 tctggtccgc ttttcgagga tcgacgccga ggcggaacgc ggcttcgacg gtctcgtcga
     1381 acttcgccgg cgcgagcgat cggactagct cgagggcgtc gcgggcagtc atcgcccggt
     1441 cgcgttcgta gcgttgcgtg gcgtcggcgt acgccttgcc gtgcttcgtc atgtcgtcct
     1501 cctcggactc cctcggtggt gggctggttc ggcggcgagg tcctccgccg aacccgacgt
     1561 tcgctcgagg cctactcgac gacttggatg cccatggaac gggcggtgcc ggccacttgg
     1621 gcgacggccc cgtcgaggtc caccgcgttg aggtcgggca tcttggtttc ggcgatcttg
     1681 cggacctggg ttttggtgac ctggccgacc ttctcgagcc ggggattttg ggctcccttt
     1741 tcgatgccgg cggcctggcg tagcaggaac ggggtgggtg gcgtcttggt gacgaaggtg
     1801 aaggaacggt cctcgaagac cgagatctcg gccgggatca cctggccgcg ccggctctcc
     1861 gtcgcggcgt tgtacgcctt gcagaaatcc atgatgttga tgccgtgcgg accgagggcg
     1921 gtgccgacgg gaggcgccgg ggtcgcttga ccggcctgga tctggatctt gacgacggcc
     1981 gccagccgtt tctttgccat ctggtgctcc tggtgtggtg gtggacagcc gggtcagagc
     2041 ttggcgactt ggccgaactc gagctcgacc ggcgtctcac gtccgaagat gttgacgagg
     2101 accttgagct tggagcggtc gacgtcgatc tcggagatgg ccccgttgaa gtccgcgaac
     2161 ggcccggtgg tgacgcggac ctgttcgcct tcctcgaatt ccacccgggg cctcaccttt
     2221 ttctcggccg cggggctgtc gggcttggcg acgccgagga tgtcctcgac ctctttgcgg
     2281 gacagggggg tgggtttctc accgcggccg ctcgggccga cgaacccggt gacgttgggg
     2341 gtgtcccgca cgactcgcca tgacccgtcg ttcatgacca tccggaccag gacgtagccg
     2401 gggaacacct tcttggggac gacgcggcgt tggccgcctt tgaactcgat gacgtcttcc
     2461 atcgggatga cgacctcgaa gatctggtcc tcggcgtgct gagatcggat ccgctgttcg
     2521 aggttctgct tgaccttgtt ctcgtagccg gcgtaggtgt gcaccacgaa ccaggcgccg
     2581 ggccggtcgt aggggctggg gggtgccggt tcggtttcgc cgccatcggt ttcctcgtcg
     2641 acgacgtcga cgagctcggc ggaggggtcg agaccgaccg gtgccggttc gggcgcctcg
     2701 gtggcgacga cgggttcggc ggcgggagcc gagcccctac gagcgccccg gcgactggtt
     2761 cgtcgtccac acgtacgcgg gctacgagaa caaggtcaag cagaacctgg ccagccggg
//
LOCUS       DATCCH010000181         7397 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_424962, whole genome shotgun sequence.
ACCESSION   DATCCH010000181 DATCCH010000000
VERSION     DATCCH010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7397
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424962"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="VIJ44_03610"
     CDS             complement(<1..409)
                     /locus_tag="VIJ44_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165020.1"
                     /translation="MRAAVSASVLAVAVLATACGGGSSGAERATTTTTTTTRVIATTT
                     TITAAGPCGGAPTVPAAVRASAVAGLNQNPTQYTVDNVSLARSDPTWGRFDALPAPGQ
                     ESVYQGGSGIVHCAANAWAVTDFGTAEVGCPGGP"
     gene            complement(483..3224)
                     /locus_tag="VIJ44_03615"
     CDS             complement(483..3224)
                     /locus_tag="VIJ44_03615"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF010707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamine synthetase]
                     adenylyltransferase/[glutamine
                     synthetase]-adenylyl-L-tyrosine phosphorylase"
                     /protein_id="HEY5165021.1"
                     /translation="MSTLTALLRPDVVAAIDSAADARLARTTLERIVEVDPDVAEAVI
                     EEPRVRDGLIAVACASRSLTNALIQDPSLVDVLRHLDGPMADLDASDPATLRRWKRRE
                     LLRIAAADLLGLMDFPTVGRRLADLADACLGAALELSGEALPFTVIGMGKLGGRELNY
                     ASDVDVLFIHDGDGADAERFARRVLAIMSEPTADGLVFRTDADLRPEGRAGALSRTID
                     GYAAWYERWARPWEFQALIKARPVAGDVELADRFLSLTRPFVWPDVLDPDAVRDVRAM
                     KARAEAEVHRRGLSDRELKRGRGGIRDVEFAVQLLQLVHGREDLRVRSATTLEALAQL
                     AGGRYVEADDARQLSAAYIFLRTVEHRLQLRDEQQTHTLPADATSRTVLARVLGYRDQ
                     PAVSALEEFETDQRRQQHVVRGIHERLFFAPILDTLAGTGPLTPEAAEERLAAFGFVD
                     ADHTRRALRELTQGLTRTSRTLQQLLPAILDWLSSAPDPDLGLLQLRRLAEGPTRATA
                     LATTFRDRPGAAERACRVLGSSHIIGDALLRQPEVVELLGDDDWLVRERSPDELRHAA
                     LAALGWRHRDTEARRAGLRRFKRRELLRVASRDVLGSATLDVTSHELASLADACVDAA
                     LQALRPTVPFAVIGMGRLGGRELSYSSDIDVLFVYDGAQPADFEAASATATAFVREIS
                     TTTAEGQTFRVDANLRPEGREGPLARSLDGYRAYYERWALTWELQALLRARPVAGDVD
                     LGNRFIALIEPFVYRDPFPDDAAREVRRIKARVERERIPPGEDPGFHLKLGKGALTDI
                     EFTVQLLQLQHGARDPSVRVAATIPALGALGNAGLLAADDVDVLAEAYTFCERARNAC
                     YLVTGRPTDSLPTGAEAARVSRLLGILHRPEAALRDEFRRVTRRARRVVERVFYGRD"
     gene            3291..4997
                     /locus_tag="VIJ44_03620"
     CDS             3291..4997
                     /locus_tag="VIJ44_03620"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEY5165022.1"
                     /translation="MSRLRVAAAQLNLVVGDLEGNAARLTDAYDRAEAAGADLVAFPE
                     LAITGYPPEDLLFRPAFVAQATEWLEKVAVHTGRTAAIVGFPQSDGHLYNAAAVCAHG
                     RVVGVYRKHLLPNYAVFDEQRYFTPETTDGPLFEIGGAKVGVTICEDAWSPTGPVLTE
                     AAAGAELIVNINASPYYAGRLREREAMLAARAADASTPILYVNLVGGQDELVFDGASL
                     LVDETGAVVARAHQFDEDLLVVDLDLRPALRRRFRSPRGRTRSTLPEIPVSEVRLGEA
                     SLTRRVEPPLPPVREVYEALVLGTRDYVTKNGFSDVLVGLSGGIDSSLVAMIAADALG
                     PEHVVGILMPSRFSSSGSVSDAAALAANLGIRTKTVPIEEAHRAFLQMLAEPFASTES
                     GLAEENLQARIRGTILMTVSNKFGWLVLTTGNKSELATGYSTLYGDMAGGFAVIKDVP
                     KTLVYALSRERNERAGRALIPDEVLDKPPSAELRPDQRDSDSLPDYAVLDRIVEDYVE
                     ADRSVRDIVDEGADAETVRRVASLIDRNEYKRRQAAPGVRVSPKAFGKDRRLPITNRW
                     PG"
     gene            5018..5908
                     /locus_tag="VIJ44_03625"
     CDS             5018..5908
                     /locus_tag="VIJ44_03625"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY5165023.1"
                     /translation="MRRYAAEGALVLAAFFFGVTFPLVHDALGDIQPFAYLVLRFAIA
                     VVVLGPFAIAIGRRRGEDSRTLLRAGLIAGGLLAAGYAAQTVGLAMVSPSTSAFITGL
                     YVVLTPVVEGIVRRAVPPRAVIAGVVLAAFGLYLLTGATVSLGAGELLTLACAALFAV
                     WIVYQGEYANRLHPIPFTAVQMAVIVVVCLPPSAAQGIGHLTGLALFAAAFTGVLCSA
                     VALSLQVWGQRRLAPSRTALILMLEPVFAGIAGYLNGERLSVVELIGAGVILAGIAVA
                     ELAASSRVRRDRPELEPHIF"
     gene            5967..6491
                     /locus_tag="VIJ44_03630"
     CDS             5967..6491
                     /locus_tag="VIJ44_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165024.1"
                     /translation="MLSIADTAAVLGHACWLEARCFELLGGWVSVVTEPEMKLLVARH
                     SRHHGWHVELLGEVLPSTRDHDPYRLVAPADALWVAAVDLVGGDAWTPTLERLVGLYQ
                     ALLPRLVVGHVEALDATSPVSDGPVARRLRMVIEDERADLAEGLAALEAFPGADVDRV
                     ADRRAVVEAALPPR"
     gene            6695..>7397
                     /locus_tag="VIJ44_03635"
     CDS             6695..>7397
                     /locus_tag="VIJ44_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY5165025.1"
                     /translation="MAKERVERDEEDLVRLYLTDIGQYPLLTKDDEVRLAQQIENGNA
                     AREDLERLPAKKLTPAKKRELRKAGREGDYAQRTFVQSNLRLVVSIAKKYQASGLPLL
                     DLIQEGNLGLMHAVEKFDWRKGFKFSTYATWWIRQAITRGIANTGRTIRLPVHAGDTL
                     ARLQKARSRLELKLGRPATLAELAVEVEMPEDKVTEALRFAAEPLSLSEPLREDGDAE
                     LGDIVEDRSVESPFEV"
BASE COUNT         1071 a         2631 c         2584 g         1111 t
ORIGIN      
        1 ccgggcctcc ggggcagccg acctcggcgg tgccgaagtc ggtgaccgcc caggcgttgg
       61 cggcgcagtg cacgatgccg gagccgccct ggtacaccga ttcctgaccg ggggccggca
      121 aggcgtcgaa gcgcccccac gtcgggtcgg agcgggccag gctgacgttg tcgacggtgt
      181 actgcgtcgg attctggttg agcccggcga cggcgctggc ccgaactgcg gcgggaaccg
      241 tcggagcacc tccgcaggga ccggcggcgg tgatggttgt cgtcgtggcg atgacccggg
      301 tcgtggtggt cgtcgtcgtg gtcgcccgct cggcgcccga actgccgccg ccgcacgcgg
      361 tcgccagcac cgcaaccgcg aggaccgacg cgctcaccgc tgcacgcacc gcctgactcc
      421 ctccacttct tccctcgacg ccgccggagc ctacgtggcg cgacaccagc tcagccttcg
      481 cgtcagtccc gtccgtagaa gacgcgttcg accacccgtc gagcccggcg ggtgacgcgc
      541 cggaactcgt cgcgcagcgc ggcttcgggg cggtggagga tcccgagcag gcggctgacc
      601 cgggcggcct ccgcaccggt cgggagggag tcggtgggcc gaccagtgac gaggtaacac
      661 gcgttccggg cccgttcgca gaaggtgtac gcctcggcga ggacgtcgac gtcgtcggcg
      721 gcgagcaggc ccgcgttccc gagcgcgccg agcgccggga tcgtcgccgc gacccggacc
      781 gacgggtccc gggcaccgtg ctggagctgg aggagctgca ccgtgaactc gatgtcggtc
      841 agggcgcctt tgccgagctt gaggtggaac ccgggatcct cgccgggggg tatccgctcc
      901 cgctctaccc gggccttgat gcggcgcacc tcccgtgccg cgtcgtcggg aaacgggtcg
      961 cggtacacga acggctcgat gagcgcgatg aaccggttcc cgaggtcgac atcgccggcg
     1021 acgggccggg ctcggagtag cgcctggagc tcccacgtca acgcccaccg ctcgtagtag
     1081 gcgcggtacc cgtcgagcga gcgggccaac ggtccttctc tcccttcggg gcgcagattg
     1141 gcgtcaaccc ggaacgtctg gccctcggcg gtcgtggtgc tgatctcgcg cacgaaggcg
     1201 gtggcggtgg ccgacgcggc ctcgaagtca gcgggttggg caccgtcgta gacgaacagg
     1261 acgtcgatgt cggacgagta ggacagctct cggcctccga gacgccccat gccgatgact
     1321 gcgaacggca ccgtgggacg caaggcctgc aacgctgcgt cgacgcacgc gtcggcgagc
     1381 gacgcgagct cgtgggacgt cacgtcgagc gtcgcggacc ccaatacatc tcgcgacgcg
     1441 acgcgaagca gttcccgccg cttgaagcgg cgcagcccgg cccggcgggc ctcggtgtcc
     1501 cgatgacgcc acccgagcgc cgccaatgcg gcgtggcgca gctcgtcggg gctgcgttcg
     1561 cgcaccagcc agtcgtcgtc gccgagaagt tccacgacct cgggctggcg gagcagcgcg
     1621 tctccgatga tgtgactcga accgagaacc cggcaggccc gctccgcggc gccgggtcga
     1681 tcccggaacg tggtcgcgag cgcggtcgcg cgggttggac cttcggcgag tcgacgcagc
     1741 tgcaggagtc ccaggtcggg atcgggcgcg ctcgacagcc agtcgagaat ggccggcagc
     1801 agctgctgga gggtgcggga agtgcgggtc agcccctggg tgagctcgcg cagcgcgcgg
     1861 cgggtgtggt cggcgtcgac gaatccgaac gcggcgagcc gttcctcggc cgcctcgggc
     1921 gtcaggggac cggtcccggc gagggtgtcg aggattggcg cgaagaacag ccgctcatgg
     1981 atgccgcgaa cgacatgctg ctgacgccgc tggtcagtct cgaactcctc gagggcgctc
     2041 accgccggtt gatcgcggta gccgagcacc cgggccagca ccgtccgact ggtggcgtcg
     2101 gcgggcaggg tgtgcgtctg ttgctcgtcg cggagctgca agcggtgctc gaccgtgcgc
     2161 aggaagatgt aggcggcgct cagctgccgg gcgtcgtcgg cttcgacgta gcggccgccg
     2221 gcgagctgcg ccaaggcttc gagcgtggtc gccgaccgga cgcgcaggtc ctcacggccg
     2281 tggacgagct gcagcagctg caccgcgaac tcgacgtcgc ggataccacc gcgcccgcgc
     2341 ttgagctccc ggtcgctcag tccccgccga tgaacctcgg cttcggcgcg ggccttcatg
     2401 gcgcgcacgt cgcggacggc gtcgggatcg agtacgtcgg gccatacgaa cggccgggtc
     2461 agagacagga accggtccgc cagctccacg tcaccggcga ccggtcgggc cttgatgagc
     2521 gcctggaact cccacggtcg ggcccaccgc tcgtaccacg cggcataccc gtcgatggtc
     2581 cgcgacaacg cgccggcccg tccctcgggg cgcagatcgg cgtcggttcg gaacaccaac
     2641 ccgtcagcgg tgggctccga catgatggcc aggacccgcc gggcgaaccg ttcggcgtcg
     2701 gctccgtcgc catcgtggat gaacaggacg tcgacgtcgc tggcgtagtt gagctcgcgt
     2761 ccgccgagct tgcccatccc gatcacggtg aagggcagcg cctcgccgct gagctcgagc
     2821 gcggcgccga ggcaggcgtc ggcgaggtcc gcgaggcgac gaccgacggt cggaaagtcc
     2881 atgagcccca acaggtcggc ggcggcgatc cgcagcagct cgcgccgctt ccagcgtcgc
     2941 agcgtcgcag gatctgaggc gtccaggtcc gccatgggcc cgtcgaggtg tcggagcacg
     3001 tcgaccagcg acgggtcctg gatgagcgcg ttcgtgagcg accgcgacgc gcaggcgacg
     3061 gcgatcagcc catcgcggac gcgaggctct tcgatcaccg cctcggcgac gtcggggtcg
     3121 acctccacga tccgttcgag cgtcgtgcgc gccaggcggg cgtccgcggc cgaatcgatc
     3181 gccgccacca cgtcgggacg gagaagcgcg gtgagggtgc tcaccggacc agtgtgtcgc
     3241 acccggttct cgggcttccg ggctgcggcg cggctcctat tcttgggtgc gtgtcacggc
     3301 ttcgtgtcgc cgccgctcag ctgaacctcg tggtcgggga cctcgagggc aacgcggcgc
     3361 gcctgaccga tgcgtacgac cgggccgagg cggccggcgc cgacctggtg gcgttccccg
     3421 agctggcgat caccggctac ccacccgagg acctgctgtt tcgcccggct ttcgtggccc
     3481 aggcgacgga atggctcgag aaggtggcgg tgcacaccgg tcggaccgcc gccatcgtcg
     3541 ggttccccca gtctgacggc cacctgtaca acgccgcggc ggtctgcgcg cacggccggg
     3601 tggtcggcgt ctaccgcaag cacttgcttc ccaactacgc ggtgttcgac gagcagcggt
     3661 acttcacgcc cgagaccacc gacgggccct tgttcgagat cggtggcgcc aaagtcggcg
     3721 tcacgatctg cgaggacgcc tggagcccga cggggcccgt cctgaccgag gccgcggcgg
     3781 gcgcggagct gatcgtgaac atcaacgcgt cgccgtacta cgcgggccgg ctacgcgaac
     3841 gcgaggcgat gctggccgcc cgggcggctg acgcgtccac cccgatcctc tacgtcaacc
     3901 tggttggcgg ccaagacgaa ctggtcttcg acggcgcatc gctcctggtc gacgagaccg
     3961 gcgccgtcgt cgcccgcgcc caccagttcg acgaggatct gctggtcgtc gacctcgatc
     4021 tccgcccggc cttgcggcgc cgattccgca gcccgcgagg acggacccgg tcgacgctcc
     4081 ccgagatccc ggtgagcgag gtccggctcg gggaagcgtc gctgacccgt cgagtcgagc
     4141 cgcccctccc cccggtccgc gaggtctacg aggccctcgt gctcggcacc cgcgactacg
     4201 tgaccaagaa cggattctcg gacgtgctcg tcggcctctc gggagggatc gactcgtcac
     4261 tcgtcgcgat gatcgccgct gacgcgctcg gccctgagca cgtcgtcggg atcctgatgc
     4321 cctcccggtt ttccagctcg gggagcgtca gcgacgcggc ggcgctcgcc gccaacctcg
     4381 ggatccgcac caagaccgtg cccatcgagg aagcccaccg cgcgttcctc cagatgttgg
     4441 ccgagccgtt cgcatcgacc gagagcggcc tcgccgagga gaacctccag gcccgcatcc
     4501 gaggcacgat cctcatgacc gtctcgaaca agttcggttg gctcgtcctc accaccggca
     4561 ataagagcga gttggcgacc ggctactcga cgctgtacgg cgacatggcc ggcggcttcg
     4621 ccgtgatcaa ggatgttccc aagacgctcg tgtacgccct ctcccgtgaa cgcaatgagc
     4681 gtgccgggcg cgccctcatc cctgacgagg tgctcgacaa gccgcccagc gccgagctgc
     4741 gaccggacca acgggacagc gattcgctgc ccgactatgc ggtgctggac cggatcgtcg
     4801 aggactacgt cgaagcagac cgctcggtgc gcgacatcgt cgacgaaggt gccgacgccg
     4861 agaccgtgcg ccgcgtcgcg tcgctgatcg accgcaacga gtacaaacgc cgtcaagccg
     4921 cgccgggcgt ccgggtatcc ccgaaggcgt tcggcaagga ccgtcgccta ccgatcacca
     4981 accgctggcc cggatagcgc cgcgcggctg aaatccggtg aggcgatacg ctgccgaagg
     5041 agccctggtc ctcgcggcgt tcttcttcgg cgtcaccttc ccgctggtcc atgacgccct
     5101 cggcgacatt cagccgttcg cgtaccttgt gctgcgcttc gcgatcgcgg tggtcgtgct
     5161 cgggccgttc gcgatcgcga tcggacgccg ccgcggcgag gactcgcgaa cgcttctccg
     5221 ggccggtctc atcgccggtg ggttgctcgc cgcgggctac gcggcccaga ccgtcgggct
     5281 ggcgatggtg tcaccgtcga cgtcggcgtt catcaccggt ctctacgtcg tactgacccc
     5341 ggtcgtcgaa ggcatcgtgc ggcgagccgt gccaccgcgt gccgtgatcg ctggtgttgt
     5401 gctcgccgcg ttcggcctct acctcctcac tggtgccacc gtctcgctcg gtgccggcga
     5461 gctgctgact ctggcgtgcg cagcactctt cgcggtgtgg atcgtctacc agggcgagta
     5521 cgccaaccgt ctgcacccga tcccgttcac cgccgtccag atggcggtga tcgtcgtggt
     5581 ctgccttccg ccatccgccg cgcaaggcat cgggcacctg acgggactgg ccctgttcgc
     5641 ggcggcgttc actggggtgc tgtgctccgc ggtcgccctc tcgctgcagg tctggggcca
     5701 gcggcgtctg gcgccgagtc gcaccgcgct gatcctcatg ctggagccgg tcttcgcggg
     5761 gatcgccggc tacctcaacg gcgagcggct cagcgtggtc gaactgatcg gcgccggcgt
     5821 gatcctcgca ggcatcgcgg tcgccgagct cgcggcgagc agccgggtcc gccgcgaccg
     5881 tcccgagctc gagccccaca tcttctgacc ccgcgccacg tcaccggtcg gaccaagccc
     5941 gcgggggcgc acctacgatc ccgcctgtgc tgagcatcgc tgacaccgct gcggtccttg
     6001 gtcacgcctg ctggctcgag gcccgctgct tcgagctgct cggcggctgg gtctcggtgg
     6061 tcaccgaacc cgagatgaag ctgctcgtcg cccggcacag ccgacaccac ggctggcacg
     6121 tcgagctcct gggcgaagtg ctgccctcga cgcgggacca tgacccttat cgtctcgtgg
     6181 ccccggccga cgccctatgg gttgccgcgg tcgatctcgt cggtggggac gcctggaccc
     6241 cgaccctcga gcgtctcgtc ggtctctatc aggcgctgct gccccgcctt gtcgttggcc
     6301 atgtcgaggc gctcgacgcc accagcccgg tgagcgacgg gcccgtcgcc cgtcgcctgc
     6361 ggatggtgat cgaagacgag cgggccgacc tggccgaggg cttggccgcg ctcgaggcgt
     6421 tccccggcgc tgacgtggac cgggtcgccg accggcgcgc cgtggtcgag gccgcgttgc
     6481 cgccccgctg agcatcccgt ccgttgccgg tggggcgcgc cgatatcctc ggaagtaggc
     6541 gccaacgctg gtgctccgac cgtgtccggc cgcttgacac ggaccgtatg gtacggggga
     6601 actcccctcc gacctgcgag aacacgcccc gccgggggcc ccgatcgccc tctgcgacac
     6661 ccaggtacga gacccccgac cacgaaaggc agcagtggcg aaggaacgag tcgagcgcga
     6721 cgaggaagat ctcgtccggc tctacctcac cgacatcggt cagtatccgc tgctgacgaa
     6781 ggacgacgaa gtccgcctgg cccagcagat cgagaacggc aacgccgccc gcgaagacct
     6841 cgagcggctc ccggccaaga agctcacccc agcgaagaaa cgggagctgc gcaaggccgg
     6901 acgcgagggc gactacgccc agcgcacctt cgtccagtcc aacctgcgcc tcgtggtgtc
     6961 gatcgccaag aagtaccagg cgtcgggtct gccgctgctg gacctcatcc aggaaggcaa
     7021 cctcggcctg atgcacgcgg tcgagaagtt cgactggcgc aaagggttca agttctcgac
     7081 ctacgccacc tggtggatcc gccaggccat cacccgaggc atcgccaaca cgggtcgcac
     7141 catccgtctc ccagtgcacg cgggcgacac cctggcccgg ctccaaaagg cgcggtcacg
     7201 tctcgagctc aagttgggac ggccggccac gctggccgag ctcgccgtcg aagtcgagat
     7261 gcccgaagac aaggtgaccg aggcgctccg cttcgccgcc gagccccttt ccctctccga
     7321 gccgctgcgc gaagacggcg acgccgagct gggcgacatc gtcgaggacc gttcggtcga
     7381 gtcgccgttc gaggtgg
//
LOCUS       DATCCH010000182         2815 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_269640, whole genome shotgun sequence.
ACCESSION   DATCCH010000182 DATCCH010000000
VERSION     DATCCH010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269640"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="VIJ44_03640"
     CDS             <1..575
                     /locus_tag="VIJ44_03640"
                     /inference="COORDINATES: protein motif:HMM:NF022805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3159 domain-containing protein"
                     /protein_id="HEY5165026.1"
                     /translation="RLAPALWAAIGITVVLVAFRAVRREPLRQALTGVLAVGISAIIA
                     VATGRAANFFAVGIFVQMLYAFAYLVSFALRWPLMGVIVGPLLGEGMSWRSDPPRRRA
                     YWKCSWIWFGVFVLRASVQLPLYLNDKVVKLGVANILMGWPLFALAALVSWLILRRVP
                     TTVPVLPEPDAGAEPAGELESEPAVRPDAG"
     gene            complement(556..1221)
                     /locus_tag="VIJ44_03645"
     CDS             complement(556..1221)
                     /locus_tag="VIJ44_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012893200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEY5165027.1"
                     /translation="MRVAIAGAGKVGRSIAAELTENGHEVMLVDKDPASVRTDSIPGA
                     QWLVADACELAVLEKADLASCQVVIAATGDDKVNLVVSLLAKTEYGVPRVVARVNHPK
                     NEWMFNESWGVDVAVSTPRMLSALVEEAVSVGDLVTLFTFRQGNANLVELTMPTSSPY
                     VGKRVGEVHWPEDVALVAILRDGRVIAPSTDDPIESGDELLFVASAESEAGLQEMLSP
                     HQV"
     gene            complement(1221..1889)
                     /locus_tag="VIJ44_03650"
     CDS             complement(1221..1889)
                     /locus_tag="VIJ44_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009714521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEY5165028.1"
                     /translation="MHVVIMGCGRVGSSLAETLDSQGVSVAVVDQETDAFRRLSATFG
                     GRTVTGVGFDREVLVDAGIERASAFAAVSSGDNSNIVAARVARETFGVENVVARIYDP
                     RRAEVYQRLGISTVATVRWTSDQILRRIRPAGAESEWTDASGTVRLAEVHVSPTWIGQ
                     LLTRIEVLTPARVAFLTRLGEGMIPGPETVLQEGDLLHVLARAADMPAIESFLDQAPP
                     HEEH"
     gene            2007..>2815
                     /locus_tag="VIJ44_03655"
     CDS             2007..>2815
                     /locus_tag="VIJ44_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009739671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY5165029.1"
                     /translation="MRSDRLGETLLPKRIALPVFASDALSSVAYATQEILIVLALAGT
                     AFLHLTWYAAAGVVTVMATVVAAYRQNVHAYPSGGGDYEVANTNLGRRFGLVVAAALL
                     VDYVLTVAVSTSAGVENLASTPLFSWMAGNRVAATLSVVVIIVLLNLRGIRESGTAFA
                     IPTYAFMFGIIAMIVVGVFRLATGANLVASSAGYPIKPELHNLGTAGIMFLGLRAFAS
                     GCTALTGVEAISNGVPAFRKPKSKNAASTLALMGGIAVSMFVGVTYLAIRL"
BASE COUNT          373 a          991 c          992 g          459 t
ORIGIN      
        1 accggctcgc tcccgcactg tgggccgcga tcggcatcac ggtcgtcctc gtcgcgttcc
       61 gggccgtgcg tcgcgagccg ttgcggcagg cgctcaccgg tgtcctggcg gtcggcatca
      121 gcgcgatcat tgccgtcgcg acgggtcggg cggccaactt cttcgccgtg ggcatcttcg
      181 tccagatgct gtacgcgttc gcctacctgg tctcgttcgc gctgcgctgg ccgctcatgg
      241 gggtcatcgt cgggccgctg ctcggcgagg ggatgagctg gcggtccgac ccgccgcggc
      301 ggcgggcgta ctggaagtgc agctggatct ggttcggcgt cttcgtcctg cgggcctcgg
      361 tgcagctgcc gctctacctg aacgacaagg tcgtcaagct cggtgtcgcg aacatcttga
      421 tgggctggcc gctgttcgcc ctcgcggcgc tcgtctcgtg gctgatcctg cggcgggtcc
      481 cgacgacggt gccggtgctg cccgagcccg acgccggagc cgagccagcc ggcgagctcg
      541 agtcggagcc tgcggtcaga cctgatgcgg gctgagcatc tcctgcaggc ccgcctcgga
      601 ctcggctgac gcgacgaaca gcagctcgtc accggactcg atcgggtcgt cggtggaggg
      661 cgcgatgacc cgcccgtcgc gcaggatcgc gacgagtgcg acgtcctcgg gccagtgcac
      721 ctcaccgact cgcttgccga cgtagggcga cgacgtcggc atcgtcagct cgaccaggtt
      781 ggcgttgccc tgccggaagg tgaacagcgt gacgaggtcg ccgacgctca ccgcctcctc
      841 gacgagggcg gacagcatcc gtggcgtgga caccgcgacg tcgacccccc acgactcgtt
      901 gaacatccac tcgttcttcg ggtggttgac ccgggcgacg acccgcggga cgccgtactc
      961 ggtcttggcc agcagcgaca ccacgaggtt gaccttgtcg tcgcccgtcg cggcgatgac
     1021 cacctggcag ctcgcgaggt cagccttctc caggaccgcg agctcgcagg cgtcggccac
     1081 cagccactgc gccccgggga tgctgtcggt gcgcaccgag gccgggtcct tgtcgaccag
     1141 catcacctcg tggccgttct cggtgagctc ggcggcgatc gaacggccca ccttgcctgc
     1201 tcctgcgatg gctacccgca tcagtgctcc tcgtggggtg gtgcctggtc gaggaagctc
     1261 tcgatggcgg gcatgtcggc ggcgcgggcg aggacgtgca gcaggtcgcc ctcctgcagg
     1321 acggtctcgg gtccggggat catgccctcg ccgaggcggg tcaagaaggc gacgcgggcc
     1381 ggcgtcagta cctcgatgcg ggtgagcagc tggccgatcc acgtggggga gacgtggacc
     1441 tcggcgagcc gtacggtgcc gctcgcatcg gtccactcgc tctcggcccc cgccggccgg
     1501 atgcggcgca ggatctggtc ggaggtccag cgcacggtcg ccaccgtcga gatgccgagc
     1561 cgctggtaga cctcggcgcg gcgcgggtcg tagatgcggg ccacgacgtt ctcgacgccg
     1621 aaggtctcgc gggccacgcg ggcggcgacg atgttggagt tgtcgccgct gctcaccgcg
     1681 gcgaaggccg acgcgcgctc gatgccggcg tcgaccagca cctcgcggtc gaagcccaca
     1741 ccggtgacgg tacggccacc gaaggtggcc gagaggcgcc ggaaggcgtc cgtctcctgg
     1801 tcgacgacgg cgacggagac cccctgggag tcgagcgtct cggccaggct cgacccgacg
     1861 cggccgcagc ccatgatcac aacatgcacg gccgatagcg tacgcacccc gagcggggcg
     1921 tgcgtacgcg gctgaccgct gcgcctacga tcgcccccgt ggtgaacttc gcgggactcg
     1981 gcaagcgcgt cctcctggga cgggcgatgc gctccgaccg tctcggcgag acgctgctcc
     2041 ccaagcgcat cgcgctgccg gtgttcgcca gcgacgcgct gtcctcggtg gcctacgcca
     2101 cgcaggagat actgatcgtc ctggcgctcg cagggaccgc cttcctgcac ctgacctggt
     2161 acgccgctgc gggggtcgtc accgtgatgg cgacggtcgt ggccgcctac cggcagaacg
     2221 tgcacgccta cccgagcggg ggcggcgact acgaggtcgc caacaccaac ctcggccgca
     2281 ggttcgggct ggtcgtcgcc gccgcgctgc tcgtcgacta cgtgctcacg gtcgcggtgt
     2341 cgacgtcggc cggcgtcgag aacctggcgt ccacgccgct gttctcgtgg atggccggca
     2401 accgggtcgc ggcgacgctc tccgtggtcg tgatcatcgt gctgctcaac cttcgcggga
     2461 tccgcgagtc gggcacggcg ttcgcgatcc ccacgtacgc gttcatgttc gggatcatcg
     2521 cgatgatcgt ggtcggcgtg ttccggctgg ccaccggcgc caacctcgtc gcgagctcgg
     2581 ccggctaccc gatcaagccg gagctgcaca acctcggcac cgccgggatc atgttcctcg
     2641 gcctgcgcgc cttcgcctcc gggtgtacgg cgctgaccgg cgtcgaggcg atcagcaacg
     2701 gcgtgcctgc cttccggaag ccgaagtcca agaacgccgc gagcaccctc gcgctcatgg
     2761 gcggcatcgc cgtgtcgatg ttcgtcggcg tcacctacct cgcgatccgg ctcaa
//
LOCUS       DATCCH010000183         2228 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_590982, whole genome shotgun sequence.
ACCESSION   DATCCH010000183 DATCCH010000000
VERSION     DATCCH010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590982"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(267..626)
                     /locus_tag="VIJ44_03660"
     CDS             complement(267..626)
                     /locus_tag="VIJ44_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006941091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HEY5165030.1"
                     /translation="MDVLNAIEQARDAVTVGRVYGEPIEHGGVTVIPAAAVQGGAGGG
                     SGTAPEGGGFGPGSASGSGSGAGWGMRARPVGAYVLRDGELRWQPALDVNRVILGGQV
                     VAVIALLTLRTWLKARR"
     gene            complement(614..1618)
                     /locus_tag="VIJ44_03665"
     CDS             complement(614..1618)
                     /locus_tag="VIJ44_03665"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5165031.1"
                     /translation="MSDEASSTLLEGRVAVVTGAGRGIGRAEAVRLAAYGARVVVNDL
                     GVATDGSETSETPADEVVTEITARGAEAVTDHHDISTTTGGAGVVQHALDTWGRLDIV
                     VNNAGIGRPRMVFNLEDEEWDDVIRVHLRGTFTVSGPACRWWRSEAKSGRDGSGRLVN
                     TATGLLLYGGAGQSNYVAAKAGVVAFTEAVATEMAPYGVTANAIMPSARTRLASIGWR
                     IGRNTDASADEFDPTDPVHVAEAVCYLASPEAAWVSGQCFQVRGGTLEHVRTWEVRNQ
                     LERHDRGWTAPDLVSEVPRLFGAGAKRSDPPPAEWREQYHARGTAATAPIREEREWMS
                     "
     gene            complement(1666..>2228)
                     /locus_tag="VIJ44_03670"
     CDS             complement(1666..>2228)
                     /locus_tag="VIJ44_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-dependent synthetase"
                     /protein_id="HEY5165032.1"
                     /translation="FEGYYKNDEANAQRVRDGFYWTGDLGYRDEQGFFYFAGRDFEWL
                     RVDGENFAAAPVERILARHPDVVLAVVYAVPDEDVGDQVMAALLLKPGVAFDAAAFDQ
                     FLTEQPDLGTKWSPRYVRITGALPVTETQKVLKRVLRRDRWEVSDPIYWRPRNGEALQ
                     PMTDADRTELHARFEARDRLDQLERV"
BASE COUNT          329 a          812 c          754 g          333 t
ORIGIN      
        1 gggaaccggc gatcccggtc gacgaacaac gcaccccggt cgtcggatcc gaacggcccc
       61 cggtcacgca gctccggctc gagccgctcg cgccacaggt ccgcgggttc catgaagtgc
      121 gaatcggcgt cgaagaccgg ccagtcgcgc accggcgcac actacgtggt cggcggccag
      181 actggctccc tcctcacact cctccccgct cgacgaccgc gagcagagtc ggtcgacctc
      241 gtcgggggtc gagggtcacc tcacgctcag cgccgagcct tcagccaggt acggagggtg
      301 agcaacgcga tcacggcgac gacctgcccg ccgaggatca cgcggttcac gtcgagcgcg
      361 ggctgccaac gaagctcacc gtcgcgcagc acgtacgcgc ccacgggacg ggccctcatg
      421 ccccagccgg cacccgaccc ggagcccgac gcggagcccg gcccgaagcc gccaccctcg
      481 ggggcggtgc cggacccgcc tccagcaccg ccttgcaccg cggcggccgg gatcacggtc
      541 acgccgccgt gttcgatggg ctcgccgtag acccgcccga ccgtgaccgc gtcgcgggcc
      601 tgttcgatgg cgttcaagac atccattccc tctcctctcg tatgggggcc gtcgccgcgg
      661 tgcctcgagc gtggtactgc tcgcgccact cggcgggcgg cgggtcggag cgtttcgcgc
      721 cggccccgaa caggcgtggc acctcgctca cgagatccgg cgcggtccag cctcggtcgt
      781 gacgctccag ctggttgcgc acctcccaag ttcggacgtg ctcgagcgtg ccccctcgca
      841 cctggaagca ctggccgctg acccaggccg cctccggcga cgccaggtag cagacggcct
      901 cggcgacgtg gacgggatcc gtcgggtcga actcgtcggc ggacgcgtcg gtgttgcgac
      961 cgatgcgcca tccgatcgac gcgagtcggg tgcgagccga aggcatgatc gcattggcgg
     1021 tgactccgta gggagccatc tcggtcgcga ccgcctccgt gaaggcgacc acgccggcct
     1081 ttgccgccac gtagttcgac tgtccggcgc cgccgtagag caagaggccg gtcgcggtgt
     1141 tcaccaatcg acccgacccg tcgcgtccag acttcgcctc cgatcgccac cagcgacagg
     1201 ccggtccact gaccgtgaac gtcccgcgga ggtggacgcg gatcacgtcg tcccactcct
     1261 cgtcttcgag gttgaagacc atccggggac ggccgatgcc ggcgttgttg accacgatgt
     1321 ccagccggcc ccacgtgtcc aaggcgtgct ggacgacccc ggcaccgccg gtggtcgtcg
     1381 agatgtcgtg gtggtcggtg acggcctcag cgccgcgggc ggtgatctcg gtgacaacct
     1441 cgtcggcggg cgtctcgctg gtctcgctgc cgtcggtcgc gacgccgagg tcgttcacga
     1501 cgacccgggc gccgtaggcc gccaggcgaa ccgcttcggc ccgcccgatg cctcgcccgg
     1561 cgccggtcac cacggcgacg cggccctcca ggagcgtgct ggatgcctcg tcgctcaccc
     1621 gatgaaccta accccaccgg tcgccgaacg gcgccgagcc tcgactcaga cgcgctcgag
     1681 ctggtcgagg cggtcgcggg cctcgaaacg ggcgtgaagc tctgttcgat cagcatcggt
     1741 catcggttgg agcgcctcac cgttgcgagg acgccagtag atcggatcag agacctccca
     1801 gcggtcccgt cgcaggaccc gcttcagcac cttctgggtc tcggtgaccg gcaaggcccc
     1861 ggtgatgcgc acgtaccgtg gtgaccactt cgtcccgaga tcgggctgct cggtgaggaa
     1921 ctggtcgaat gccgcggcgt cgaaggccac gcccggtttc agcagcagtg cggccatgac
     1981 ctggtcgccg acgtcttcgt cggggacggc atagacgacg gcgaggacca cgtcggggtg
     2041 acgggcgagg atccgctcga ccggcgcggc ggcgaagttc tccccgtcga cgcggagcca
     2101 ttcgaaatcg cggcccgcga agtagaagaa gccttgttcg tcgcggtagc cgaggtcccc
     2161 ggtccagtag aagccgtccc gcacccgctg cgcgttcgct tcgtcgttct tgtagtagcc
     2221 ctcgaagc
//
LOCUS       DATCCH010000184         3162 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_342538, whole genome shotgun sequence.
ACCESSION   DATCCH010000184 DATCCH010000000
VERSION     DATCCH010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342538"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VIJ44_03675"
     CDS             <1..232
                     /locus_tag="VIJ44_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165033.1"
                     /translation="GLAMFGLACLLVTARRSPASMALLGLLAASFVYYIADYAGILVN
                     FPLPSFQANDMVDAVLAASAGLGAAQLGAPHG"
     gene            229..951
                     /locus_tag="VIJ44_03680"
     CDS             229..951
                     /locus_tag="VIJ44_03680"
                     /inference="COORDINATES: protein motif:HMM:NF023670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinofuranosyltransferase"
                     /protein_id="HEY5165034.1"
                     /translation="MSEPLRARLGRGGITAVFSVAAVVVSFSLVQTAIKAIPYVEQQR
                     QSHEPTQALRDFQRATGGHTTNSVVLTDVTELPVYLPLYVFNWWDAHYMNPPARFNDR
                     SAFLKRLSEETDPQAFALALLHNVYDRIDYVALRPSPAGPFQYQFADDAFPRGVVERT
                     FTYQRALFGSAAFHRVDTVSFTVFVLLRHHDPLASLQSCPAQPRRASCRVLGVVSRRY
                     HGDVDNAVLTLASRWATARRTG"
     gene            complement(959..1249)
                     /gene="raiA"
                     /locus_tag="VIJ44_03685"
     CDS             complement(959..1249)
                     /gene="raiA"
                     /locus_tag="VIJ44_03685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEY5165035.1"
                     /translation="MNVIVRGKNVSVPSTLRMAAQRKLGRLERVARDASRAEVDFSEE
                     RNPRIRQRHRCAVVVHRPYELLTAHAAAPDPEAALDLVVDKLRHQAAQHKDR"
     gene            complement(1383..2837)
                     /gene="ahcY"
                     /locus_tag="VIJ44_03690"
     CDS             complement(1383..2837)
                     /gene="ahcY"
                     /locus_tag="VIJ44_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018827118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEY5165036.1"
                     /translation="MTTTAPATRSTDRPDSKVADLSLAEFGRKEIELAEHEMPGLMTM
                     RERHSATKPLAGARITGSLHMTVQTAVLIETLLALGAEVRWASCNIFSTQDHAAAAIA
                     ARGIPVFAWKGETLEEYWWCTEQALRWPADADGHDGPTLILDDGGDATLAVHKGVEFE
                     RAGSVPDPTDTDSEEWRVVLGMLRRVLAQDDRVWHRMAEGLRGVTEETTTGVHRLYQM
                     HDAGTLLFPAINVNDSVTKSKFDNLYGCRHSLVDGICRATDVMLAGKVAVVCGYGDVG
                     KGCAQSLKGQGARVVVTEIDPICALQAAMEGYQVLTLEDAVDTADLFVTATGNLNIIT
                     LDHMLRMKHNAIVGNIGHFDNEIDMAALAALPGVERINIKPQVDEWHFPDGHSIIVLA
                     EGRLLNLGCATGHPSFVMSNSFTNQVLAQIELYTHPDFYERRVYTLPKHLDEEVARLH
                     LDKLGVRLTTLSEEQASYLGVPASGPYKPDHYRY"
     gene            complement(2942..>3162)
                     /locus_tag="VIJ44_03695"
     CDS             complement(2942..>3162)
                     /locus_tag="VIJ44_03695"
                     /inference="COORDINATES: protein motif:HMM:NF021916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEY5165037.1"
                     /translation="RLSARIDRTTELIEEALLQVLEVQAEGEGRLAQLLDLMYIGDWV
                     SYYLALDNDVDPGPIDAIAQLKAAVAAD"
BASE COUNT          458 a         1054 c         1106 g          544 t
ORIGIN      
        1 cgggctggcg atgttcgggc tggcgtgtct cctggtcacc gcccgccgct cgccggcttc
       61 catggcgctg ctggggctgc tcgcggcatc gttcgtctac tacatcgccg actatgccgg
      121 gatcctcgtg aacttcccgc tcccgtcgtt ccaggccaac gacatggtcg acgccgtcct
      181 cgcagccagc gccggtctgg gcgcagccca gcttggcgca ccgcacgggt gagcgagccg
      241 ctgcgcgctc ggctcgggcg aggcggtatc accgcggtct tttccgtcgc ggcggtggtc
      301 gtctcgttca gcctggtgca gaccgcgatc aaggccatcc cgtacgtcga gcagcagcgc
      361 cagtcccacg agccgaccca agccttgcgg gacttccagc gggcgaccgg cggtcacacc
      421 accaacagcg tcgtcctgac cgacgtcacc gagctgccgg tctacctgcc cctctatgtc
      481 ttcaactggt gggacgccca ctacatgaac cctccggccc ggttcaacga ccgcagcgcg
      541 ttcctgaaac gactgtccga ggagaccgac ccgcaagcct tcgcgctggc gctcctccac
      601 aacgtctatg accggatcga ctacgtcgcc ctgcgaccga gcccggcggg gccgttccag
      661 taccagttcg ccgacgacgc gttccctcgt ggcgtcgtgg agcgaacgtt cacgtaccag
      721 cgggccctgt tcggatcggc ggcgttccac cgcgtcgaca ccgtgagctt caccgtgttc
      781 gtgctcctac gccaccatga cccgctggcg agcctccagt catgccccgc ccagccacgg
      841 cgcgcgtcgt gtcgggtcct cggtgtggtg tcgcgccgct accacggcga cgtcgacaac
      901 gcggtcctca cgttggcgtc acggtgggcg accgcccggc gaacagggtg acgcccgctc
      961 agcggtcctt gtgctgggcc gcctgatggc gaagcttgtc gaccacgagg tcgagcgcgg
     1021 cctcggggtc cggggccgcg gcatgggcag tcaggagctc gtagggccgg tgcacgacga
     1081 cggcgcaccg gtggcgctgg cggatccggg ggttgcgctc ctcggagaag tcgacctcgg
     1141 cccgggaagc gtctcgggcg acgcgctcga gtctccccag cttgcgctga gccgccatcc
     1201 gcagcgtgga cgggacggac acgttcttgc cgcgcacgat cacgttcatc ggcgcctccc
     1261 caatgccggt gggtcgggca ccgcggaggg tacggcgccg ggcggcgccg atcaatggcc
     1321 agccggcccg gcccgggccc ggccccgggg aaccccgagg gtcaagcccg gtccgcggac
     1381 ggtcagtacc ggtaatggtc gggcttgtac gggcccgagg ccggcacacc gaggtagctg
     1441 gcctgctcct cgctgagcgt ggtgagccgc actccgagct tgtcgaggtg gaggcgggcc
     1501 acttcctcgt cgaggtgctt gggaagcgtg tacacccggc gctcgtagaa gtccgggtgc
     1561 gtgtagagct cgatctgggc cagcacctgg ttcgtgaagc tgttcgacat cacgaagctg
     1621 gggtgaccgg tcgcgcaccc gaggttcaag agtcgacctt cggcgaggac gatgatcgag
     1681 tgcccgtccg ggaagtgcca ctcgtcgacc tgcggcttga tgttgatccg ctcgacaccg
     1741 ggcagggcgg ccagggccgc catgtcgatc tcgttgtcaa agtgaccgat gttccccacg
     1801 atcgcgttgt gcttcatccg aagcatgtgg tcgagggtga tgatgttgag gttcccagtc
     1861 gcggtgacga acaagtcggc ggtgtcgacc gcgtcttcga gcgtcagcac ctggtagccc
     1921 tccatcgcgg cctgcagcgc gcagatgggg tcgatctcgg taacgacgac ccgagctcct
     1981 tggcccttga gcgactgggc gcagcccttg ccgacgtcgc cgtagccgca tacgacggcg
     2041 accttgccgg cgagcatcac gtcagtggcc cgacagatgc cgtccacgag cgagtgccgg
     2101 cagccgtaga ggttgtcgaa cttcgacttc gtgacggagt cgttgacgtt gatcgccggg
     2161 aacagcagtg tccccgcgtc gtgcatctgg tacaggcgat gcaccccggt ggtggtctct
     2221 tcggtgacgc cgcgcagacc ttcggccatc cggtgccaca ccctgtcgtc ctgggcgagg
     2281 actcggcgca gcatgccgag caccacccgc cattcctcgg agtcggtgtc ggtcggatcg
     2341 ggaacggatc cggcgcgctc gaactcgacg cccttgtgca cggcgagagt ggcgtcacca
     2401 ccgtcgtcga ggatgagggt tgggccgtcg tggccgtccg cgtcagcggg ccaacgcaac
     2461 gcctgctcgg tgcaccacca gtactcctcg agggtctcgc ccttccatgc gaacactggg
     2521 atgccgcgcg ccgcgatcgc cgcggccgcg tgatcctggg tcgagaagat gttgcaggac
     2581 gcccagcgga cctcagcgcc gagggcaaga agggtctcga tgagcaccgc ggtctggacg
     2641 gtcatgtgca gcgagccggt gatgcgggcc ccggcgaggg gtttcgtcgc gctatggcgt
     2701 tcccgcatgg tcatcagccc gggcatctcg tgctcggcga gctcgatctc tttgcgcccg
     2761 aactcggcga gggacaggtc cgcgactttg gagtcgggtc ggtcggtcga tcgggtggcc
     2821 ggggcggtgg tggtcatgtg actcctcctg agatgctcgg gcgcgacgac acgcgcctga
     2881 atgcttgggg agtcggcccg gatcaccggg tgacagcagg tcagcccgac tggaaaccac
     2941 tctagtcggc ggcgacggcg gccttgagtt gggcgatggc gtcgatcggg ccggggtcga
     3001 cgtcgttgtc gagggcgagg taataggaca cccagtcgcc gatgtacatg aggtcgagca
     3061 gctgggcgag acggccttcg ccttcggcct gcacctcgag gacctggagg agggcctctt
     3121 cgatgagctc ggtggtgcgg tcgatgcggg ccgagaggcg ct
//
LOCUS       DATCCH010000185         2468 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_310687, whole genome shotgun sequence.
ACCESSION   DATCCH010000185 DATCCH010000000
VERSION     DATCCH010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310687"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="VIJ44_03700"
     CDS             <1..422
                     /locus_tag="VIJ44_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEY5165038.1"
                     /translation="FCTIASTATPTWMEGTALPTTPEATRERMLCEWDSQFPSHGMHL
                     RSIYRDSWLCTVYEPSTDGQPTGLEAIVGDRVLKPCGIHYEGTEGELYNVDDDPHQFH
                     NLWEDAGYQSLRADLTADLYDSLPEARPNPLLVEAPT"
     gene            complement(522..884)
                     /locus_tag="VIJ44_03705"
     CDS             complement(522..884)
                     /locus_tag="VIJ44_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007266064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY5165039.1"
                     /translation="MAEIVRKRFEDADETRPFQDKGQVELVNIDGGIVGRATFQPGWK
                     WSEHVKPIAGTDSCQAAHLGYVVSGRQKVLMDDGTELEIGPGDVISIPAGHDGWTVGD
                     EPCVVLDFAGMSNYAKPS"
     gene            1310..2467
                     /locus_tag="VIJ44_03710"
     CDS             1310..2467
                     /locus_tag="VIJ44_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020525663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY5165040.1"
                     /translation="MRLAFTPEQEALQVELRDYFTRLVQEVEGHPDSESTYLRYIRRM
                     GEDGWLGLGWPKEYGGQGRGPIEQMLFVEESHWAGVPLPLLTLNSVGPTLMALGTADQ
                     KRQLLPRILRGQLHFAIGYTEPGAGTDLAALETRAVRDGDEYVVNGQKLFTSAIEHAD
                     YVWLAVRTDPDAPKHKGLSILLVPTDAAGFHWVPLRTMAGEFTSATFYDDVRVPDTSL
                     VGDENQGWKLITNQLNHERVAICPASGTLRSIQEVRRWAQQTRKPDGQRVIDAEWVQT
                     TLARVTARAEFLKLLNWKVAWAAGRGLRPADASATKVFGTEFALEAYRLLMEIVGPAA
                     YLEEGAPGAVLAGRLERDYRSQVIFTFGGGTNEIQRDIIAMAGLGMPRAPR"
BASE COUNT          441 a          825 c          794 g          408 t
ORIGIN      
        1 ccttctgcac catcgccagc accgcaacac caacctggat ggaaggcacc gcgctgccaa
       61 ccacgccaga ggcaacccgc gagcggatgc tgtgcgaatg ggacagccag ttcccgagcc
      121 acggcatgca tctccgatct atctaccgcg acagctggct ctgcaccgtg tacgagccaa
      181 gcaccgatgg tcagcccacc ggtctcgaag caatcgtcgg cgatcgcgtc ctcaagccgt
      241 gcggcatcca ctacgagggc accgagggcg agctgtacaa cgtggacgac gatccccacc
      301 agttccacaa cctgtgggag gacgccggct accagtcgct gcgcgccgac ctcaccgccg
      361 acctctacga cagcctcccc gaggctcgcc ccaacccact gttggttgag gcaccgactt
      421 aggtcgcgag gacgaaactg gaccggttgg gttcggccgc ggtcggcgat cgacttcgat
      481 actggcaatc cccgctcagg gagcgatgca acgtcggggt gttagctcgg cttcgcgtag
      541 ttggacatcc ccgcaaagtc gaggacaacg cacggttcgt cgccgaccgt ccaaccgtcg
      601 tgtccagcgg gaatcgagat cacatcgccg ggaccgatct cgagctcggt cccgtcgtcc
      661 atcagaacct tctgacgtcc ggaaacgacg tatcccagat gggccgcctg gcagctgtcc
      721 gtgcccgcaa tcggtttgac gtgctcggac cacttccagc cgggttggaa cgttgcccga
      781 ccgacgatgc cgccgtcgat gttgacgagc tccacttggc ccttgtcctg aaacgggcgg
      841 gtctcgtctg cgtcctcgaa gcgcttgcgc acaatctctg ccacggtgac tctccctcgg
      901 ttagtcggcg actcggtcct gtccggtgtc tgccggcccc atccttgccg ctgctgggga
      961 ccggggaaaa cggtgccggt tggctgtcga gaccagggtc cttcggaccg gtgtcggcct
     1021 caaccgaacc tctcgagttc gacgattcgg ggctgtctcg cagctgctcg cgccacatca
     1081 cccggctgat gccgaacccg tccggcccgc gacttgcctc ggctgatgaa atggtcgcca
     1141 gtaacctcct ggccgtgccg cgtccgagga gccgagcgcg acgaagaccg cgatcctgcg
     1201 gggaaccgcc gaccggcgaa ccggtgggat gtcacccacc ggcgatgcaa aacgatttgc
     1261 ggggtcggcg gtctgcggtc ggcggtcgac ggacgaggag cacctggtca tgaggttggc
     1321 gttcacaccc gaacaagagg ctctacaggt cgagctccgc gactacttca cccgactggt
     1381 ccaagaggtc gaaggccacc ccgatagcga gtcaacctac ctccgctaca tccgccggat
     1441 gggtgaggac ggctggcttg ggctgggctg gccaaaggaa tacggcggtc aaggccgcgg
     1501 cccgatcgaa cagatgctct tcgtcgagga atcgcattgg gccggggtac cccttccgct
     1561 cttgaccctc aacagtgtcg gcccgacgct gatggcgctg ggcaccgccg accagaagcg
     1621 ccagctgctt ccccggatcc tccggggtca gctgcacttc gcgatcggat acaccgaacc
     1681 cggggccggc accgacctcg ccgcgctgga gacgcgagcg gtccgcgacg gcgatgagta
     1741 tgtcgtcaac ggccagaagc tgttcacgag cgcgatcgag cacgccgact acgtgtggct
     1801 cgccgtccgt accgatccgg acgccccgaa gcacaagggt ttgtcgatcc tgctcgtccc
     1861 gacggacgcc gccggattcc attgggtgcc gctgcgcacc atggccggcg agttcaccag
     1921 cgcgacgttc tacgacgacg tccgggttcc tgacacgagt cttgtcgggg acgaaaacca
     1981 gggatggaaa ttgatcacca atcagctcaa ccacgaacgg gtggccatct gtcccgcgtc
     2041 ggggacgctg cgcagcattc aggaagtgcg gcgctgggct caacagacgc gcaaacccga
     2101 cggacagcgg gtcatcgacg cggagtgggt gcagacgact ttggcccggg tcacggcccg
     2161 ggccgaattc ttgaagctgc tcaactggaa ggtcgcatgg gcggctggca ggggactacg
     2221 gccggccgac gcgtcggcga cgaaggtctt cgggacggag ttcgcgctcg aggcctaccg
     2281 gctgttgatg gagatcgttg gcccggcggc ctacctcgag gagggggcac cgggagcggt
     2341 cctggccggg aggctcgaac gcgactaccg atcgcaggtg atcttcacgt tcggtggtgg
     2401 caccaacgag attcagcgcg acatcattgc catggccggg ctgggcatgc ctcgcgcacc
     2461 ccgctgac
//
LOCUS       DATCCH010000186         3271 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_104177, whole genome shotgun sequence.
ACCESSION   DATCCH010000186 DATCCH010000000
VERSION     DATCCH010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104177"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..976
                     /locus_tag="VIJ44_03715"
     CDS             <1..976
                     /locus_tag="VIJ44_03715"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEY5165041.1"
                     /translation="DYAAPLSNELVYSMAVERLLDIDVTPRATWIRMMLVELNRMSSH
                     LLFQASNGMDVGAVSMMVYGWRERELTLRFLEKITGLRMNHNFIRPGGVAADLPDGWQ
                     HDAEELCRTVEKGVREYSDLLSENPIFLERLMGVGTISTEDALALGVTGPILRATGFP
                     WDLRKAQPYLAYDEVEFDVMYGRNGDCYDRFRLRLEEILESIKIVRQCIGAMPLGDYR
                     VQDRKVTPPPRARIDESMEALIHHFKLFTEGFRVPPGETYASVESPRGEIGCYLVSDG
                     TGKPVRLHIRGPSLYNLQSLGTIAVGGMVSDAVAIISSLDPIMGETDR"
     gene            976..1476
                     /locus_tag="VIJ44_03720"
     CDS             976..1476
                     /locus_tag="VIJ44_03720"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEY5165042.1"
                     /translation="MGFTDADRRSAREIISRYPHPKSALLPLLHLAQDRDGWITPEAM
                     EEIAAMLDLTSALVLGVCSFYSMLKREPVGRLIVSVCTNLSCMVNGGTDLHTQLRAKY
                     AGDPDVSVEEVECIAHCDHAPAFQVNYDFHGPGDLDGAVDLIERYKDGKLALRTISGT
                     RAEEVR"
     gene            1476..2819
                     /gene="nuoF"
                     /locus_tag="VIJ44_03725"
     CDS             1476..2819
                     /gene="nuoF"
                     /locus_tag="VIJ44_03725"
                     /inference="COORDINATES: protein motif:HMM:NF010120.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEY5165043.1"
                     /translation="MAAATTGGPHETPVVTQFLRDRPDDSWTIEAARAHNHAYDTAKQ
                     AFGMERDAIIQAVTDSVLRGKGGAGFGTGQKWSFLPKDVFPRYLCVNADEGEPCTFKD
                     HMLIERDPHQLIEGIIIAAYAIQTDLAFVYLRGEFALGAERLGQAIRDAYAQGFLGEN
                     ILGSGFTLDVVLHRGAGCYIAGDETGLLSSLEGERAMPRIKPPFPAAQGVYAAPTIVN
                     NVETISTVPHIVRNGAAWYNAMGVNRSTGTRIFSVSGHVERPGNYEVELGMTFRELID
                     GLAGGVRGGRPMKFFIPGGASSPWLLPQHLDSPLDMDYVQGELKSMLGSGAIMVFDES
                     TDPLLIAWRLAKFFAHESCGKCTPCREGSGWIAKVLYRMAHGHGRPEDLDLLLSFGNN
                     IVPGLNAPFAQTTICALGPSTMSPVVSLDRWFRDEITERMRADTQRRRTLEVVPA"
     gene            2837..>3271
                     /locus_tag="VIJ44_03730"
     CDS             2837..>3271
                     /locus_tag="VIJ44_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013875152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY5165044.1"
                     /translation="MSQWPSQYRYTIPVPPGPDLVTLTIDGQAIQAPRGELLIKVAQQ
                     HGTYIPRFCWHERLKPVGMCRMCLVEVEGMRGLQISCATPVNDGMVVRTTSDPVKTAQ
                     DGILEFLLINHPLDCPVCDRGGECPLQDQTLAFGPGESRFIEE"
BASE COUNT          567 a         1168 c          995 g          541 t
ORIGIN      
        1 ggattacgcg gcgccgctct cgaacgagct cgtgtacagc atggcggtcg agcgcctgct
       61 cgacatcgac gtgaccccgc gcgccacgtg gatccgcatg atgctcgtcg agctcaaccg
      121 catgtcgtca cacctgctgt ttcaagcctc gaacgggatg gacgtcggcg cggtgtcgat
      181 gatggtctac ggctggcgcg agcgcgagct gacccttcgc ttcctcgaga agatcaccgg
      241 ccttcgcatg aaccacaact tcatccgccc gggtggggtc gccgccgacc tgcccgacgg
      301 atggcagcac gacgccgaag agttgtgccg caccgtcgag aagggggtgc gcgagtactc
      361 ggacctgctc agcgagaacc ccatcttcct cgaacggctc atgggtgtcg ggaccatctc
      421 caccgaggac gcgctggccc tgggcgtcac cggcccgatt cttcgcgcca ccgggttccc
      481 gtgggatctc cgcaaggccc agccgtacct cgcgtacgac gaggtcgaat tcgatgtcat
      541 gtacggccgc aacggcgact gttacgaccg gttccggctc cgcctcgagg agatcctcga
      601 gtcgatcaag atcgtccgcc agtgcatcgg cgccatgccg cttggcgact accgggttca
      661 ggaccgcaag gtcaccccac cgccccgagc ccgcatcgac gaatcgatgg aagcgctcat
      721 ccaccatttc aagctgttca ccgaggggtt ccgggtcccc cccggcgaga cctacgccag
      781 cgtcgagtcg ccgcgcggtg agatcggctg ctacctcgtg tcggacggga ccggaaagcc
      841 ggtccgcctg cacattcgcg gtccgtccct ctacaacctt cagtctctcg ggaccatcgc
      901 ggtcgggggc atggtctccg acgccgtcgc catcatctcc agcttggatc cgatcatggg
      961 cgagaccgac cgctgatggg cttcaccgac gccgaccgtc gctcggcccg cgagatcatc
     1021 agccgctacc cccaccccaa gtccgcgctg ctgcccctcc tgcacctcgc gcaggaccga
     1081 gacgggtgga tcacacccga ggcgatggaa gagatcgcgg cgatgctcga tttgacgtcc
     1141 gcgctcgtcc tcggcgtctg ctccttctac tcgatgctca aacgcgagcc ggtgggccgg
     1201 ctcatcgtct cggtgtgcac caacttgtcg tgcatggtca atggcgggac cgatctccac
     1261 acccagctgc gagccaagta cgccggcgac cccgacgtct ccgtcgaaga agtcgagtgc
     1321 atcgcccact gcgaccacgc gcctgccttc caggtcaact atgacttcca cggcccagga
     1381 gacctcgacg gcgccgttga cctcatcgag cggtacaagg acgggaagct cgcgttgcga
     1441 accatctctg gaacccgtgc cgaagaggtc cggtgatggc cgccgccacc accggtggac
     1501 cgcacgagac gccggtcgtc acccagttcc tccgcgaccg gcccgacgac tcgtggacga
     1561 tcgaagccgc ccgcgcccac aaccacgcct acgacacggc caagcaggcg ttcggcatgg
     1621 agcgggacgc catcatccaa gcggtcaccg actccgtgct tcgaggcaag ggcggcgccg
     1681 gattcggcac cgggcagaaa tggtcgttcc tcccgaaaga cgtgtttccc cgctacctgt
     1741 gcgtcaacgc cgacgagggc gaaccctgca cgttcaagga ccacatgctc atcgagcggg
     1801 atccgcacca gctcatcgag ggcatcatca tcgccgcgta cgccatccaa actgacctgg
     1861 cgttcgtgta cctgcgaggc gagttcgcgc tcggcgcgga acggctcggg caagcgatcc
     1921 gcgacgcgta cgcgcaaggt ttcctcggcg agaacatcct tggatcaggc ttcaccctcg
     1981 acgtcgtcct gcaccggggc gccggctgtt acatcgctgg cgacgagacc ggattgctgt
     2041 ccagcctcga gggtgaacgc gccatgccgc gcatcaagcc gcccttcccg gcggcgcaag
     2101 gggtctatgc cgcgccgacg atcgtgaaca acgtcgagac gatctcaacc gtcccgcaca
     2161 tcgtgcgcaa cggcgcggcg tggtacaacg cgatgggtgt caaccgctcc accgggacgc
     2221 gcatcttctc ggtatccggc catgtcgaac ggcccggcaa ctacgaggtc gagctcggca
     2281 tgacgttccg cgagctgatc gacgggcttg ctggcggggt gcgcggcggt cggcccatga
     2341 agttcttcat ccccggtggc gcatcctccc cgtggttgct gccccagcac ctcgactcgc
     2401 ctctcgacat ggactacgtc caaggagagc tgaagtcgat gctcggttcc ggggccatca
     2461 tggtcttcga cgaatccacc gacccgctgc tcatcgcgtg gcgtctagcc aagttcttcg
     2521 cccacgagtc gtgcgggaaa tgcacgccct gccgcgaggg atcaggctgg atcgccaagg
     2581 tcctctaccg gatggcccac ggtcacgggc ggccggagga cctcgacttg ttgctctcct
     2641 ttggcaacaa catcgtcccc ggcctcaacg cgccattcgc gcagaccacc atctgcgcac
     2701 tcggaccaag cacgatgagc cccgtcgtga gcctcgaccg gtggttccgc gacgagatca
     2761 ccgaacggat gcgcgccgac acccagcgac gccgcaccct cgaggtcgtg ccagcatgac
     2821 gacgagcgct gtgcccatgt cgcagtggcc gtcgcagtac cggtacacga tcccggtgcc
     2881 gcccggtccc gacctcgtca ctctcaccat cgacggccaa gcgattcaag ccccccgagg
     2941 cgagttgctc atcaaggtgg cgcagcagca cggcacgtat atcccacggt tctgctggca
     3001 cgaacgcctc aagccggtcg gcatgtgtcg catgtgcctc gtcgaggtcg aggggatgcg
     3061 gggtctgcag atctcgtgcg ccacccccgt caacgacggc atggtcgtac ggaccaccag
     3121 cgacccggtc aaaaccgccc aggacggcat cctcgagttc ctcctcatca accacccgct
     3181 cgactgcccg gtctgcgacc gcggcggcga gtgccccctg caggaccaga cgctcgcgtt
     3241 cgggcccggt gagtcgcggt tcatcgagga g
//
LOCUS       DATCCH010000187         3254 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_477680, whole genome shotgun sequence.
ACCESSION   DATCCH010000187 DATCCH010000000
VERSION     DATCCH010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477680"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..548
                     /gene="rplL"
                     /locus_tag="VIJ44_03735"
     CDS             165..548
                     /gene="rplL"
                     /locus_tag="VIJ44_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006548361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEY5165045.1"
                     /translation="MATLTKDELLDVFKNMTVLELNEFLKAFEDEFGVTAAAPVAVAA
                     GPVSAGEAPAAEEQDEFDVILTAAGDKKIQVIKEVRALTTLGLKEAKDLVDGAPNPVL
                     EKATKEDAEKAKAQLEAAGATVDVK"
     gene            778..>3254
                     /gene="rpoB"
                     /locus_tag="VIJ44_03740"
     CDS             778..>3254
                     /gene="rpoB"
                     /locus_tag="VIJ44_03740"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015805302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEY5165046.1"
                     /translation="MPRLASRERLSFSRLPQVLELPDLIAVQRQSFQWFIEEGIAEVL
                     ADISPIEDFTGTLKLELADHVFDPPKHSEEECRDKDMTYARPLFVTARFMNSTTGEIK
                     EQTVFLGDFPMMTARGTFIINGTERIVVSQLVRSPGVYFGINPDKTQPEKDVVDAKMI
                     PGRGAWLEFEVDKKDIAYVRIDRKRKQPVTILLKALGFGESPEELASLVLDPNGQPYQ
                     SMLNTLDKDNTEDAEAAFIDIYRKLRPGEPPTPDSARQLLENLFFNPKRYDMAKVGRH
                     KVSKKLRDEYAKLPLKRYDLVVPNADPEAGPVDFQLTRADVLATVSYLVKLHNNDPEY
                     FPDDIDHFGNRRVRSVGELIQNQVRVGLSRMERVVRERMTTQDVEAITPQTLINIRPV
                     VAAIKEFFGSSQLSQFMDQTNPLAGLTHKRRLSALGPGGLSRERAGFEVRDVHPSHYG
                     RMCPIETPEGPNIGLIGYLATYARINRFGFIETPYRNVVNGRATEDIEYLAADDEDRY
                     VIAQANAALDTNGRFLDERVLVRAKRGELAYVDPHEVDYMDVSPKQIVSVATSLIPFL
                     EHDDANRALMGANMQRQAVPVLRPTAPYIGTGVEERAARDAGDLILAQGNGRVLDVNG
                     DSIVVEYESGTRRGRVTYRLAKFRRSNQGTCINQKPVVATGDVIKENDVLADGPSTQD
                     GELALGKNLLVAFMSWKGHNFEDAIVISERLVRDDVLTSIHIEEHEIDARDTKLGAEE
                     ITRDIPNLSEDILKDLDEEGIVRVGAEVGPGDILVGKVTPKGETELTAEEKLIRAIFK
                     EKAREVRDTSLKVPHGESGKVIDVRVFS"
BASE COUNT          623 a         1181 c          978 g          472 t
ORIGIN      
        1 cctgagaccg ccggcgccga cgccgggcca tcaccggcac ccgacgacgc cgtcagcccc
       61 gccgccgccg tcgacgaggc cccgagcgcc gacgccgaca ccaacgaatc cgagacccaa
      121 gacccggcga cgcccgacga cgccgaatca gaggagaact gaccatggcc accctgacca
      181 aagacgagct cctggacgtg ttcaagaaca tgaccgtcct cgagctcaac gagttcctga
      241 aggccttcga ggacgagttc ggcgtgaccg ccgcggcgcc ggtcgcggtc gccgccggtc
      301 cggtctccgc cggcgaggcg cccgccgccg aggagcagga cgagttcgac gtgatcctca
      361 ccgccgccgg cgacaagaag atccaggtca tcaaagaggt ccgggcgctc accacgctgg
      421 gcctgaagga agccaaggac ctcgtcgacg gcgcccccaa tccggtgctc gagaaggcca
      481 ccaaagaaga cgccgagaag gccaaagccc agctcgaagc tgctggcgcc accgtcgacg
      541 tcaaatagcg ccccccggcc tggcgcggcc ggcccttggg aacccttgac ccggcagggg
      601 ggccgttcta gggtcgcgcc cgacatcagt ccggcccctc cggggtctgg tcgctgccgt
      661 ttgtttgcag ccagatccgg aggcgagtaa catccggcta gccgtgtctg cccgctcgag
      721 ctactgctgt tctccctgac cgctgcccgc gcgcgcctcg ctctctaagg agtggtattg
      781 cctcgcctcg cgtcccgcga acgcctgtcg ttctcacgtt tgccccaggt gctggagctg
      841 cccgacctca tcgccgtgca gcgccagtcg ttccagtggt tcatcgagga ggggatcgcc
      901 gaggttctcg ccgacatctc acccatcgag gacttcaccg ggaccctgaa actcgagctc
      961 gccgaccacg tctttgaccc gcccaagcac tccgaggagg agtgccgcga caaggacatg
     1021 acctacgccc ggccgctgtt cgtgacggcc cggttcatga actccaccac cggggagatc
     1081 aaggagcaga cggtcttttt gggtgacttc ccgatgatga ccgcccgggg caccttcatc
     1141 atcaacggca ccgagcggat cgtggtctcc cagttggtcc ggtccccggg cgtctacttc
     1201 gggatcaacc ccgacaagac ccagcccgaa aaggacgtcg tcgacgccaa gatgatcccc
     1261 ggacggggtg cctggctcga gttcgaggtc gacaagaaag acatcgccta cgtccggatc
     1321 gaccggaagc gcaagcagcc agtcacgatc ctcctcaagg ccctcggctt cggggaatcc
     1381 cccgaggagc tcgccagcct ggtcctcgac ccgaacgggc agccgtacca gtcgatgctc
     1441 aacaccctcg acaaggacaa caccgaagac gccgaggcgg cgttcatcga catctaccgc
     1501 aagctgcgcc ccggcgagcc gccgacaccg gactcggccc gccagctcct cgagaatctc
     1561 ttcttcaacc cgaagcgcta cgacatggcc aaagtcgggc gccacaaggt ctccaagaag
     1621 ctgcgcgacg agtacgccaa gttgccgctc aagcgctacg acctggtcgt ccccaacgcc
     1681 gatcccgaag ccggaccagt ggacttccag ctcacccggg ccgacgtcct ggccaccgtc
     1741 tcgtacctgg tgaagctcca caacaacgac cccgagtact tcccggacga catcgaccac
     1801 ttcgggaacc gtcgtgtccg ctccgtcggc gagctcatcc agaatcaggt ccgagtcggc
     1861 ctctcccgca tggagcgcgt ggttcgcgag cgcatgacca cccaggacgt cgaggccatc
     1921 acaccccaga ccctgatcaa cattcgtccg gttgtcgcgg cgatcaaaga gttcttcggg
     1981 tccagccagc tgtcgcagtt catggaccag accaacccgc tcgccggact gacccacaag
     2041 cgccgcctct ccgcgctcgg gccgggcggg ctgtcgcgcg aacgcgccgg cttcgaggtc
     2101 cgagacgtgc acccgagcca ttacgggcgc atgtgcccca tcgagacgcc ggaaggcccg
     2161 aacatcggcc tcatcgggta cctcgcgacg tacgcgcgga tcaaccggtt cggcttcatc
     2221 gagacgccgt accgcaatgt cgtcaacggt cgcgccaccg aagacatcga atatctcgcc
     2281 gccgacgacg aggaccgcta cgtcatcgcc caggcgaacg cggcgctcga caccaacggc
     2341 cgcttccttg acgagcgcgt cctcgtgcgc gccaagcggg gtgagcttgc ctacgtcgac
     2401 ccgcacgagg tcgactacat ggacgtctcg cccaagcaga tcgtctcggt cgccacctcg
     2461 ctcatcccgt tcctcgagca cgacgacgcc aaccgggcgc tgatgggtgc caacatgcag
     2521 cgccaagccg tcccggtgct gcgccccacc gccccctaca tcggcaccgg ggttgaggag
     2581 cgcgccgctc gcgacgccgg cgatctcatc ctcgcccagg gcaacggtcg agttctcgac
     2641 gtgaacggtg acagcatcgt cgtcgagtac gagagcggca cccggcgcgg ccgggtcacg
     2701 taccgcctcg cgaagttccg acgttccaac cagggcacct gcatcaacca gaagccggtg
     2761 gtcgccaccg gggacgtcat caaagagaac gatgtgctcg ccgacggtcc gtcgacccag
     2821 gatggtgagc tcgcactcgg caagaacctc ctcgtcgcct tcatgtcctg gaagggacac
     2881 aacttcgagg acgccatcgt catctccgag cgactggtgc gcgacgacgt gctcacctcg
     2941 atccacatcg aggagcacga gatcgacgcc cgagacacca agctcggcgc cgaggagatc
     3001 acccgcgaca tcccgaacct ctccgaggac attctcaaag acctcgacga ggaagggatc
     3061 gtgcgggtcg gcgccgaggt gggtccgggc gacatcctcg tcggcaaggt gacgccgaag
     3121 ggcgagaccg agctgacggc cgaggagaag ctgatccgcg ccatcttcaa ggagaaggcg
     3181 cgcgaggtcc gcgacacctc gctcaaggtg ccccacggcg agtccggcaa ggtcatcgac
     3241 gtccgggtct tctc
//
LOCUS       DATCCH010000188         2497 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_217721, whole genome shotgun sequence.
ACCESSION   DATCCH010000188 DATCCH010000000
VERSION     DATCCH010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217721"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..2215
                     /locus_tag="VIJ44_03745"
     CDS             155..2215
                     /locus_tag="VIJ44_03745"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEY5165047.1"
                     /translation="MGYRLGVDVGGTFTDLLCVTPDGAVVLDKTPTTADDQSTGVMNG
                     VAQLAEHLGLSVSELCNQLDILVHGTTTADNTMIEMNGAPTGLLVTEGHRDEIEMRRV
                     HKEQIWDPSYPAPPPIARRRARIAIPERLNFRGDVIRALDEDAVRRGVRRLQQLGVRS
                     VAIMFLFSFVNPDHERRAAQIVREEFPDVEHISMSHEVMARGPEFERVSTTLVNAYIA
                     PKIASYVDSLQSKLRAAGYAGSLLLMQATGGVMPPEYVSKRAVSLLASGPTGGVMGAA
                     LAAAQVGVRDFVAIDMGGTSFDVCLVRDGQPEIKTDWNWRYRYYLGLPMVDVQSVGAG
                     GGSIARVRQGALLVGPESAGSSPGPVSYGLGGRSATVTDADAVLGYLPVTEFAGGRMR
                     LDLDGASAAIARDVADPLGLDPVDAAWGIERIVNANMANATRRVLASHGADPRTLEMI
                     AYGGNGPVHAWAIARELGIERVLVPKAAPAFSALGVLVADYVIDLVRAYVVPLSQVEL
                     TRVRTLMVELRDEASKELEPAGLTRDDVELGLFAQMAYPGQNFDMSVPVPEAENLGET
                     GLLDLAERFHDQHERDRGFSFRSQQPLLRGVRIVARGRTPKPDHLAELGTLKVADSAR
                     RGSRPAHFGDGYVDTAVYDGAALGVGAEVHGPALVEEPFTVIVVPPGSRARVDAAGNY
                     ALEL"
     gene            2217..>2497
                     /locus_tag="VIJ44_03750"
     CDS             2217..>2497
                     /locus_tag="VIJ44_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165048.1"
                     /translation="MAATAGFFTANEHATVAAACNRLVPPLDAHPGGAALGCADYIDG
                     LLAAFASDPPRIWAGGPFSGRAGGDAGFETFLTLSRVEELAWRTRIEGS"
BASE COUNT          381 a          945 c          806 g          365 t
ORIGIN      
        1 cgagtacgtc agcgtcgacg cggcgcctcg tgactacggc gtcgtgctcc ggggatcgct
       61 cgacgacctc accctcgcgg tggacgagga cgcgaccaac cagcttcggg cctcccgccg
      121 ctcctgaccg cagctccggg ccacaccgcc acccatgggc tatcgactcg gcgttgacgt
      181 cggaggcacg ttcaccgacc tgctctgcgt gaccccggac ggggcggtcg tgctcgacaa
      241 gaccccgacc accgccgacg accagtccac cggggtcatg aacggtgtcg cccagctcgc
      301 ggagcacctc gggctcagcg tctccgagct gtgcaaccag ctcgacatcc tcgtgcacgg
      361 cacaaccacc gccgacaaca ccatgatcga gatgaacggt gcgccgactg gcctgctcgt
      421 caccgaaggg caccgcgacg agatcgagat gcgccgcgtc cacaaggagc agatctggga
      481 cccgtcgtac ccggcgccac ctccgatcgc tcggcgtcga gcccggatcg cgataccgga
      541 acggctcaac ttccgaggtg acgtcatccg agccctcgac gaggacgcgg tccgtcgagg
      601 agtgcgacgc ttacagcagc tcggcgtccg gtcagtcgcc atcatgttcc tcttctcgtt
      661 cgtgaacccc gatcatgaac gccgcgcggc ccagatcgtc cgggaggagt tccccgacgt
      721 cgagcacatc tcgatgtcgc acgaggtcat ggcccgaggc cccgagttcg aacgggtctc
      781 gaccacgctc gtcaacgcgt acatcgcgcc caagatcgcc agctacgtcg acagcctcca
      841 atcgaagctg cgcgccgccg gctacgccgg gtcgctgctc ctcatgcaag ccaccggagg
      901 tgtcatgcct cccgagtacg tctccaaacg agccgtgtcg ctgctcgcgt ccggacccac
      961 tggaggggtg atgggcgcag cgctcgcagc cgcccaggtg ggggtgcgtg acttcgtcgc
     1021 catcgacatg ggcggcacca gcttcgacgt ctgcctggtg cgtgacgggc agcccgagat
     1081 caagaccgac tggaactggc ggtaccggta ctacctcggc ctgcccatgg tcgacgtcca
     1141 aagcgtcggg gccggcggtg gctccatcgc ccgcgtccgc caaggcgcgc tcctcgtcgg
     1201 acccgagagc gccgggtcct caccgggccc ggtcagctac ggcctcggtg gccggagcgc
     1261 caccgtcacc gacgccgacg cggtgctcgg ctacctgccc gtcaccgaat tcgcgggcgg
     1321 gcgcatgcgt ctcgatctcg acggggccag cgccgcgatc gcccgcgacg tcgccgaccc
     1381 gctcggtctc gaccccgtcg acgccgcgtg gggcatcgaa cggatcgtga acgccaacat
     1441 ggccaacgcc actcgccggg tgctggcctc ccatggcgct gaccctcgca ccctcgagat
     1501 gatcgcctac ggggggaacg gaccggtgca cgcctgggcc atcgcacgag agctgggcat
     1561 cgaacgggtc ctggtcccga aggccgcgcc ggcgttctcg gccctcggtg tgctcgtcgc
     1621 cgactatgtc atcgacctcg tccgcgcgta cgtcgtgccg ctctcacagg tcgagctcac
     1681 gagggtgcgg accctgatgg tcgagttgcg cgacgaggcg agcaaggagc tcgagccggc
     1741 cgggctcacc cgcgacgacg tcgagctcgg cctgttcgcc cagatggcct accccggtca
     1801 gaacttcgac atgagcgtcc ccgttcccga agccgagaac ctcggtgaga ccggactcct
     1861 ggacctcgcc gagcgattcc acgaccagca cgaacgggac cgagggttct cgttccgcag
     1921 ccaacagccg ctgctgcgcg gcgttcgcat cgtcgctcgg ggccggaccc ccaagcccga
     1981 tcacctcgcc gagctcggaa ccctcaaggt cgccgactcc gcccgccgag gcagccgccc
     2041 cgcgcacttc ggggacggat acgtcgacac tgcggtgtac gacggcgcgg cgctcggggt
     2101 cggcgccgag gtgcacggtc ccgccctggt cgaggagccg ttcaccgtga tcgtcgtccc
     2161 ccccggttcc cgcgcccggg tcgacgcggc gggcaactac gccctcgaac tctgacgtgg
     2221 cggccaccgc gggcttcttc accgccaacg agcacgcgac cgtcgccgcg gcttgcaatc
     2281 ggctggtccc acccctcgac gcgcaccccg gcggagccgc gctcggctgc gccgactaca
     2341 tcgacgggct cctcgccgcc ttcgcgtccg atccgccccg catctgggcc ggcggcccgt
     2401 tctcgggacg cgccggcggc gacgccggct tcgagacctt cctgaccttg agccgcgtcg
     2461 aggagctggc ctggcggacc cgcatcgaag ggtcaca
//
LOCUS       DATCCH010000189         5785 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_519262, whole genome shotgun sequence.
ACCESSION   DATCCH010000189 DATCCH010000000
VERSION     DATCCH010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5785
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519262"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VIJ44_03755"
     CDS             <1..272
                     /locus_tag="VIJ44_03755"
                     /inference="COORDINATES: protein motif:HMM:NF015559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HEY5165049.1"
                     /translation="AAFLRAGPPGVPFDLVVADPPYEEAAPEIAALLDFLGQPGWLTP
                     SARVVLETPTRGGAVSGTAGPGWETRSERKYGDTLLTTIGPVPRI"
     gene            285..773
                     /gene="coaD"
                     /locus_tag="VIJ44_03760"
     CDS             285..773
                     /gene="coaD"
                     /locus_tag="VIJ44_03760"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018162748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEY5165050.1"
                     /translation="MATALCPGSFDPVTNGHVDIIERTARHFDDVIVAVIRNPQKAQS
                     LFTLAERQEMLHEVTAHVPNLHIEFFKGLMVDFAREHGAEAIVKGLRAVSDFDYELQM
                     AQMNQRLSGIDTFFISTSPQHSFLSSSLVREVARFGGDVSSMVPPTVAKRLAERFGGG
                     TG"
     gene            770..1291
                     /locus_tag="VIJ44_03765"
     CDS             770..1291
                     /locus_tag="VIJ44_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165051.1"
                     /translation="MSEDEARTLEALLGELRDMVEAARTMPMSASVLVNRDEALALLD
                     EASRAFPAELRHARWLLKERQEYLDGAKRESDGLLESARVQAERMVERSDIVREARHS
                     AQEVLADAEAEARRLHHQAEDYVDQRLAALETLLDKTITGVRKGRERLQVDLPEPEPV
                     DEDEVPIFDQDDS"
     gene            1397..1867
                     /locus_tag="VIJ44_03770"
     CDS             1397..1867
                     /locus_tag="VIJ44_03770"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEY5165052.1"
                     /translation="MSPIRIDVADLLSHPGARRHVNLTEPVEGLAATAARVVDPIDVD
                     LDLDHISEGIVVRGAVRATWRGECSRCLRDLAQPLELHVDELFEPSPVPGETYPLEGH
                     EIDLDQLVRDAVLLELPLAPHCDSPCDPTPIGDAADTDDPPADPRWAVLSELEI"
     gene            1886..2065
                     /gene="rpmF"
                     /locus_tag="VIJ44_03775"
     CDS             1886..2065
                     /gene="rpmF"
                     /locus_tag="VIJ44_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013980117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEY5165053.1"
                     /translation="MAVPKRKTPRSRTHSRRSANRGVEAPARSLCPHCGAVKLPHRVC
                     GNCGHYKGVQRIEVD"
     gene            2070..3074
                     /gene="plsX"
                     /locus_tag="VIJ44_03780"
     CDS             2070..3074
                     /gene="plsX"
                     /locus_tag="VIJ44_03780"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEY5165054.1"
                     /translation="MPGPACTVAVDAMGGDRGPGEIVTGAVQAATELGIGVALVGRAE
                     EITPHLPVPAPTDVTVIDARDVIAMDDEPVSAVRKLKDSSIVRCARAVRDGEADAMVS
                     AGNTGAVTTAAVLRLRRFRGVPSPAIAVPIPVPDHHPQLLVDAGAMVDVSPERLVQYA
                     RMATAYAQARYGLDTPSVALLSNGEEAGKGDELRKRAHELLRDEPGFIGNVEGRDFMH
                     PDRADVIVTDGFTGNVALKSLEGALRSLASVVFGVLDATPEAREASTVLLPMLLAAAE
                     IYDPDVTGGAVLLGVDGVCIISHGSSSARAITNAVRVAADCVATEVVAHMKKAVTHAG
                     "
     gene            3064..3375
                     /locus_tag="VIJ44_03785"
     CDS             3064..3375
                     /locus_tag="VIJ44_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEY5165055.1"
                     /translation="MPAETHVERTPPGPDEVFTLVRERLAAILEIPEDRITLDARFAD
                     DLDADSLALIELVEELEEELGERTVGFRVDDEDLADLHTVREAVDYVAARLHAGEDST
                     P"
     gene            3372..4088
                     /gene="rnc"
                     /locus_tag="VIJ44_03790"
     CDS             3372..4088
                     /gene="rnc"
                     /locus_tag="VIJ44_03790"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018655288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEY5165056.1"
                     /translation="MSDDARARLEAALGRDFHDPARLDAALIHRSYCAEHPAANSNER
                     LEFLGDAVLGLVVTVYVFEEFPDLPEGELAKLRASVVNAEVLAQVATEIDLGPALLLG
                     KGEDGSGGRDKPSILADAIEAVIAAVYLDGGWDAAHDLVLGLLEDRIRAAATGPGGQD
                     YKTRLQELAARRFDQLPRYQVRAEGPDHSKQFFATVRLNGETRGEGEGRSKKQAEQAA
                     ARAAWESLHDTEERSDAGAA"
     gene            4072..4908
                     /gene="mutM"
                     /locus_tag="VIJ44_03795"
     CDS             4072..4908
                     /gene="mutM"
                     /locus_tag="VIJ44_03795"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEY5165057.1"
                     /translation="MPELPEVEVVRRDLEREIVGKRVKGVEVDGMRTVRRHHNRKQFI
                     TRLEGKKFTGVERRGKYLLCRLEGGDVLIIHLGMSGQLLRAKTARESRSKHTHVVITF
                     TQGGQLRFIDPRTFGEMFVTEVDALNKVSELNHLGIDPLENAMSWEQFGQLISQRHAK
                     LKPLLMDQKFLAGIGNIYSDEILWGAGLRWDRMSDSLSAQEVRRLYRAMVEILQDAVK
                     YRGSSLADEQYVDLFGKLGDYQNHHKVYARDGETCRRCRHVIRRERVGGRSTFYCEAC
                     QV"
     gene            4914..5189
                     /locus_tag="VIJ44_03800"
     CDS             4914..5189
                     /locus_tag="VIJ44_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019356860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEY5165058.1"
                     /translation="MAERVRRRLVISGQVQGVFFRDRCQGEARAHDVAGWVTNRPDGR
                     VEAVLEGDPVAVATVEAWCRRGPRRAAVTHVDTVDEAPTGATGFQIR"
     gene            5274..>5785
                     /locus_tag="VIJ44_03805"
     CDS             5274..>5785
                     /locus_tag="VIJ44_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY5165059.1"
                     /translation="MFLKSLTLKGFKSFADKTTLDFEPGVTVVVGPNGSGKSNLVDAV
                     AWVLGAQGPRTLRGARMDDVIFAGTPSRAALGRSEVSLTIDNTAHLLPIEFSEVTITR
                     TLFRTGESEYQLNDVPCRLLDIQELLSDTGIGRQQHVIVGQGQLDTILNASPADRRAI
                     VEEAAGILKFR"
BASE COUNT          956 a         2256 c         1771 g          802 t
ORIGIN      
        1 tcgccgcgtt cctccgggcc ggcccgcccg gggtgccgtt cgatctcgtc gtcgccgacc
       61 ctccgtatga ggaggcggcc ccggagatcg cggcgctcct cgacttcctc ggtcagcccg
      121 gttggctcac cccgtcggcc cgcgtggtgc tcgagacccc cacccgaggc ggcgcggtgt
      181 ctggcaccgc ggggccgggg tgggagaccc ggtcggaacg caagtacggc gatacgctcc
      241 tcaccacgat cggccccgtc ccccgtatct gaggaggcga ccgcgtggcc accgcgctgt
      301 gtcccgggtc gttcgacccg gtcaccaacg gacatgtcga catcatcgag cggaccgcac
      361 gccacttcga cgacgtcatc gtggcggtga tccgcaaccc ccaaaaggcc cagtcgctgt
      421 tcaccctcgc cgagcgccag gagatgctcc acgaggtcac cgcccacgtc cccaaccttc
      481 acatcgagtt cttcaagggc ctcatggtcg acttcgcccg cgagcacggc gccgaggcga
      541 tcgtcaaggg cctccgggcg gtgtcggact tcgactacga gctccagatg gcccagatga
      601 accagcggct ctcgggcatc gacaccttct tcatctcgac cagcccccag cactcgttcc
      661 tctcgtcgag tctcgtgcgc gaggtggccc gcttcggcgg cgacgtctcg agcatggtgc
      721 ccccgaccgt cgcgaaacgc ctcgccgaac gcttcggcgg cgggacggga tgagcgagga
      781 cgaggcccgc acgctcgagg cgctcctcgg cgagctgcgc gacatggtcg aagccgcccg
      841 caccatgccg atgtcggcgt cggtgctcgt caaccgcgac gaagccctcg ctctcctcga
      901 cgaggcgtcg cgcgccttcc ccgccgagct gcgccacgcc cgctggctcc tcaaggaacg
      961 ccaggagtac ctcgacggcg ccaaacgcga atccgacggg ctcctcgaat cggctcgggt
     1021 ccaagcggag cggatggtgg aacgcagtga catcgtccgc gaggctcgtc acagcgccca
     1081 ggaggtcctc gccgacgccg aagccgaagc ccgccggctc caccaccaag ccgaggacta
     1141 cgtcgaccag cgcctcgccg ccctcgagac cctcctcgac aagaccatca ccggtgtccg
     1201 caagggacgc gagcgcctcc aggtcgacct gcccgaaccc gagcccgtcg acgaggacga
     1261 agtcccgatc ttcgaccaag acgacagctg accccaccac cgaacccgaa aaatccgaca
     1321 aatcggatct ttcgcttagc cgggcggaga agcggtcgct ggtatcctca tacaccgctc
     1381 ctgctgaggc caacccatgt cccccatccg aatcgacgtc gctgacctgc tcagccatcc
     1441 cggggcccgc cgtcacgtca acctgaccga gcccgtcgag ggcctcgccg ccaccgcggc
     1501 ccgcgtcgtc gaccccatcg acgtggacct cgacctcgac cacatttccg aagggatcgt
     1561 cgtgcgaggc gccgtccgag ccacctggcg aggcgagtgc agccgctgtc tccgcgacct
     1621 cgcccaaccg ctcgagctcc acgtcgacga gctgttcgaa cccagccccg tccccggtga
     1681 gacctacccc ctcgaaggcc acgagatcga cctcgaccag ctggtccgag acgcggtgct
     1741 cctcgagctg cccctcgccc cgcactgtga ctcgccgtgc gacccgaccc cgatcggcga
     1801 cgccgccgac accgacgacc cgcctgcgga tccccgctgg gcggtcctct ccgaactcga
     1861 gatctgacct ccccgaggag acctgatggc tgtccccaag cgcaagaccc cacgatcgcg
     1921 gacccacagc cgccggtcgg cgaaccgcgg cgtcgaggcc cccgcccggt cgctgtgtcc
     1981 gcattgcggc gcggtcaagc tgccccaccg ggtctgtggc aactgcggcc actacaaggg
     2041 cgtgcagcgc atcgaggtcg actaacaccg tgcctggacc ggcctgcacg gtcgcggtcg
     2101 acgccatggg cggcgaccgc ggcccaggcg agatcgtcac cggcgcggtc caggccgcca
     2161 ccgagctcgg catcggcgtc gcgctcgtcg gccgggccga agagatcacg ccccacctcc
     2221 cggttccggc cccgaccgac gtcacggtca tcgacgctcg tgacgtcatc gccatggacg
     2281 acgagccggt cagcgcggtc cgcaagctca aggactcctc gatcgtgcgt tgcgcccgcg
     2341 ccgtgcgcga cggcgaggcc gacgccatgg tcagcgccgg gaacaccggg gcggtcacca
     2401 ccgccgcggt gctgcgactc cgaaggttcc gtggcgtacc cagccccgcc atcgcggtcc
     2461 ccatcccggt ccccgaccac caccctcagc tcctcgtcga cgccggcgcc atggtcgacg
     2521 tctcaccgga acggctggtc cagtacgccc gcatggccac cgcgtacgcc caagcccggt
     2581 acgggctcga cacccccagc gtcgccctgc tctccaacgg cgaggaggcc ggcaaaggcg
     2641 acgagcttcg caaacgggcc cacgagctgc tgcgcgacga gcccggcttc atcggcaacg
     2701 tcgaaggacg cgacttcatg catccggacc gggccgacgt gatcgtcacc gacgggttca
     2761 ccggcaacgt ggccctcaag agcctcgaag gcgcgctgcg ctccctcgcc agcgtcgtat
     2821 tcggcgtcct cgacgccacc cccgaagccc gcgaagcctc caccgtcctg ctcccgatgc
     2881 tcctcgcggc cgccgagatc tatgaccccg acgtcaccgg cggtgcggtg ctactcggcg
     2941 tcgacggggt ctgcatcatc tcgcacggct cctcgtcagc ccgggcgatc accaacgccg
     3001 tgcgcgtcgc cgccgactgc gtcgccaccg aggtcgtcgc ccacatgaag aaggcggtta
     3061 cccatgccgg ctgaaaccca cgtcgaacgc accccgcccg gccccgacga ggtgttcacc
     3121 ctcgtccgcg agcgcctcgc cgcgatcctc gagatccccg aggaccgcat caccctcgac
     3181 gcccgcttcg ccgacgacct cgacgccgac tctctcgcgc tcatcgagct cgtcgaagag
     3241 ctcgaggagg agctcgggga acgcaccgtc gggttccgcg tcgacgacga agacctcgcc
     3301 gacctccata cggtccgcga agccgtcgac tacgtcgccg cccgcctcca tgccggggag
     3361 gactccacgc catgagcgac gacgcgcggg cgcgcctcga ggccgcgctc ggccgcgact
     3421 tccacgaccc ggcccgcctc gacgcggccc tgatccaccg ctcctactgc gcggagcatc
     3481 ccgcggcgaa ctccaacgag cgcctcgaat tcctgggcga cgccgtcctc ggcctcgtcg
     3541 tcaccgtcta cgtcttcgag gagttccccg acctccccga aggcgagctc gccaagctgc
     3601 gggcctcggt cgtgaacgcc gaggtcctcg cccaggtcgc gaccgagatc gacctcggcc
     3661 ccgccctgct gctcgggaaa ggcgaggacg gctccggcgg gcgcgacaag ccgtcgatcc
     3721 ttgccgacgc catcgaagcc gtgatcgccg cggtgtacct cgacggcggc tgggacgccg
     3781 cccacgacct cgttctcggc ctcctcgaag accggatccg cgccgcggcc accggccccg
     3841 gcggccagga ctacaagacc cgactccaag agctggcggc gcgccgcttc gaccagctgc
     3901 cccgctacca ggtccgcgcc gaagggcccg accactcgaa acagttcttc gccaccgtgc
     3961 gcctcaacgg cgagacccgc ggcgaaggcg aagggcgctc caagaagcag gctgagcagg
     4021 ccgccgcccg ggccgcctgg gagagcctgc acgacaccga ggaacggtca gatgccggag
     4081 ctgcctgaag tcgaggtcgt tcgccgcgac ctcgaacgcg agatcgtcgg caagcgggtc
     4141 aaaggcgtcg aagtcgacgg gatgcggacc gtgcgccgcc accacaaccg caagcagttc
     4201 atcacccgcc tggaaggcaa gaagttcacc ggcgtcgaac gccgcggcaa atacctcctg
     4261 tgtcgcctcg aaggcggcga cgtgctcatc atccacctcg gcatgtcggg ccagctgctg
     4321 cgagccaaga ccgcccgcga atcgcgcagc aagcacaccc acgtcgtcat cacgttcacc
     4381 caaggcggcc agctccgatt tatcgacccc cgcaccttcg gtgagatgtt cgtcaccgaa
     4441 gtcgacgcgt tgaacaaggt ctcggagctc aaccatctcg gcatcgatcc cctcgagaac
     4501 gcgatgtcgt gggagcagtt cggccagctc atctcccaac gccacgccaa gctcaagccc
     4561 ctcttgatgg accagaagtt cctggccggt atcggcaaca tctacagcga cgagatcctc
     4621 tggggcgccg gcctgcgatg ggaccgcatg agcgactcgc tctcagccca agaggtccga
     4681 cgcctctacc gggcgatggt cgagatcctc caggacgcgg tgaagtaccg gggctccagc
     4741 ctcgccgacg agcagtacgt cgacctgttc ggcaagctcg gcgattacca gaatcaccac
     4801 aaggtgtacg cccgagacgg cgagacgtgt cgccgctgcc gccacgtcat ccgacgcgaa
     4861 cgcgtcgggg gccgctccac cttctattgc gaggcgtgcc aggtctgacg ccggtggccg
     4921 aacgagtccg tcgccgcctg gtgatctcag gccaggtcca aggcgtcttc ttccgtgacc
     4981 gctgccaagg cgaggcccgg gctcatgacg tcgcgggctg ggtcaccaac cggcccgacg
     5041 ggcgcgtcga agcggtcctc gaaggcgacc cggttgccgt cgccacggtc gaggcttggt
     5101 gccgccgcgg cccccgccga gccgcggtca cccacgtcga caccgtcgac gaggccccga
     5161 ccggcgccac cggcttccag atccgctgaa cgcggcccgc tgaccaggcg cgacggcgta
     5221 acccatcggc ggccgacctc gtcgctacga tcccgaagaa gagaggggcc gccgtgttcc
     5281 tgaagtcact caccctgaag ggcttcaagt cgttcgccga caagaccacg ctggacttcg
     5341 agcctggcgt caccgtcgtt gtcggcccca acggctccgg caagtccaac ctcgtcgacg
     5401 cggtggcgtg ggtcctcggc gcccagggac cccggacgct gcgcggcgcc cggatggacg
     5461 acgtcatctt cgctggcacc ccgtcccgag ccgcgctcgg ccgctccgag gtgtcgctga
     5521 cgatcgacaa caccgcccat ctcctcccca tcgagttcag cgaggtgacg atcacccgca
     5581 ccctgttccg caccggcgaa tccgagtacc agctcaatga cgtcccgtgc cgcctcctcg
     5641 acatccaaga gctgctctcc gacaccggca tcggccgcca acagcacgtc atcgtcggcc
     5701 agggtcagct cgacaccatc ctcaacgcgt cgcccgccga ccggcgcgcc atcgtcgagg
     5761 aggccgccgg gatcctcaag ttccg
//
LOCUS       DATCCH010000190         2916 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_519359, whole genome shotgun sequence.
ACCESSION   DATCCH010000190 DATCCH010000000
VERSION     DATCCH010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519359"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /locus_tag="VIJ44_03810"
     CDS             <1..842
                     /locus_tag="VIJ44_03810"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY5165060.1"
                     /translation="ANGSNEVLQTLLLGYGGPGRRVLVFEPTYALHAHIARITATEVV
                     EAERSADFLVSATEAGLVIAAERPAVVFLCSPNNPTGTVEPRATVEALLAAVVDHGPA
                     LLVVDEAYGEFAGWSAVELVADEVPLLVARTFSKVWSLAALRLGFAVAPAPVVAQLDE
                     VVLPYHLSATTQLAGLEALTFDAEMRARVDALVAERHRLASALTRLPDLTVYPSGANF
                     VLVRVDGDAHALWQRLVDRGVLVRDFSAAPRLEGCLRITVGTPAEDDALLDALRESLE
                     EAA"
     gene            839..1426
                     /gene="hisB"
                     /locus_tag="VIJ44_03815"
     CDS             839..1426
                     /gene="hisB"
                     /locus_tag="VIJ44_03815"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEY5165061.1"
                     /translation="MSTARRGERHRQTKETHVDLVLVVDGSGAATASTGIPFFDHMLE
                     QLGKHGGFDLSVDAEGDLAVDLHHTVEDVGIAFGEALRDALGDKAGVRRFASALVPLD
                     EALVQVALDLSGRPFLVYDVDPISEWIGTFDPQLCEEFWRAVAFAAGITLHLRSVSGR
                     NGHHIIEASFKAVARSLRDAVRVEGTGIPSTKGTL"
     gene            1429..2160
                     /gene="hisA"
                     /locus_tag="VIJ44_03820"
     CDS             1429..2160
                     /gene="hisA"
                     /locus_tag="VIJ44_03820"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEY5165062.1"
                     /translation="MTVYPAIDVRAGRAVRLTRGDFADETIYADDPVAVARAFAAAGS
                     DWIHVVDLDAARSGEPANLAVVEAITAAVPCRVQAGGGVRSIEAAGELLLAGAARVVV
                     GTAAVEHPTLVDDLCAAHPGQVAVGFDARGRDVATRAWTASSGQDLVALARRFEDRGV
                     AALIVTEIGRDGTMTGPDLDQLAAVLDATALPVIASGGVGHLGDLEALTALSAGGRHL
                     AGVIVGRALYEGRFTLAEALAVAAP"
     gene            2216..2428
                     /locus_tag="VIJ44_03825"
     CDS             2216..2428
                     /locus_tag="VIJ44_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165063.1"
                     /translation="MDDAATIDEGFLRRYRELLDAEDEAFDELEHAYEDGERARFEHD
                     VERWRIAVERRHTFLERHGVTPLVTS"
     gene            complement(2443..>2916)
                     /locus_tag="VIJ44_03830"
     CDS             complement(2443..>2916)
                     /locus_tag="VIJ44_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165064.1"
                     /translation="FAALPPAGVTCLRFNFRGVEGSEGTHDQGRGEQADVRAAVRDLA
                     PRLAPTSPLVLTGWSFGADVALSVVDDRIDAWYAIAPPLRFTTGLDILAGDRRPKLLA
                     LAEHDEFRSPTEVQAAAASWVATETEVVRGASHFFVGRTDRLVELAVGLVGRLAS"
BASE COUNT          407 a         1119 c          989 g          401 t
ORIGIN      
        1 gcgccaacgg ctccaacgag gtcctccaga cgttgctgct cggctacggc ggccccggcc
       61 ggcgggtgct ggtcttcgag ccgacctacg cgctgcacgc ccacatcgcg cggatcaccg
      121 ccaccgaggt ggtcgaggcc gaacgcagcg ccgacttcct cgtctcggcg accgaagcgg
      181 gcctggtgat cgcggccgag cgtcccgcgg tcgtcttcct ctgcagcccg aacaacccga
      241 ccggcaccgt ggagccgcgc gccaccgtcg aggccctcct cgccgcggtc gtcgaccacg
      301 gccccgcgct cctcgtcgtc gacgaggcct acggcgaatt cgccggctgg agcgcggtcg
      361 agcttgtcgc cgacgaggtc ccgctgctcg tggcccgcac cttctcgaag gtgtggtccc
      421 tggccgcgct gcggctcggg ttcgcggtcg cacccgcacc ggtcgtcgcc cagctggacg
      481 aggtcgtcct cccatatcac ctctcggcga ccacccagct cgcgggactc gaggcgctga
      541 ccttcgacgc cgagatgcgc gcccgcgtcg acgcgctcgt ggcggagcgg catcgtctcg
      601 cctcggcgtt gacccgcctc cccgacctca ccgtctaccc gtcgggcgcg aacttcgtcc
      661 tggtccgggt cgacggtgac gcccacgcgc tgtggcagcg acttgtcgac cgcggggtac
      721 tcgtccgcga cttctcggcc gcgcctcgcc tcgaagggtg tctgcggatc accgtcggga
      781 cgcccgccga ggacgacgcg cttctcgacg cgctgcgcga atcgcttgag gaggccgcgt
      841 gagcaccgcc cgacgcgggg aacgacaccg ccagaccaag gagacccacg tcgacctcgt
      901 gctcgtcgtc gacgggtccg gtgccgcgac ggccagcacc ggcatcccgt tcttcgacca
      961 catgctggaa cagctcggca agcacggcgg gttcgacctg agcgtcgacg ccgaaggaga
     1021 ccttgccgtc gacctgcacc acaccgtcga agacgtcggc atcgcgttcg gggaggcgct
     1081 gcgcgacgcg ttgggcgaca aggccggggt gcgtcgcttc gcgtcggcgc tcgtccccct
     1141 cgacgaggcc ctggtccagg tggcactcga cctctccggg cgaccgtttc tcgtgtacga
     1201 cgtcgacccg atcagcgagt ggatcggcac cttcgacccc cagctctgcg aggagttctg
     1261 gcgggcggtg gcgttcgccg ccggcatcac cctccacctg cgctccgtgt cgggccgcaa
     1321 cggccaccac atcatcgagg cgtcctttaa agctgtcgcc cggtcccttc gcgacgcggt
     1381 gcgggtcgaa ggcaccggga tcccctcgac caagggcacc ctctgagcct gaccgtctac
     1441 ccggcaatcg atgtgcgcgc cgggcgcgcc gtgcgactca cccgaggcga cttcgccgac
     1501 gagacgatct acgccgacga tccggtcgcg gtggcgcgtg ccttcgccgc cgcgggcagc
     1561 gactggatcc acgtcgtcga cctcgacgcc gcccgcagcg gcgaacccgc caacctcgcg
     1621 gtcgtcgagg ccatcaccgc cgcggtcccg tgccgggtcc aggccggcgg cggggtccgc
     1681 agcatcgaag cggccggcga gctgctcctg gccggcgcgg cccgagtcgt ggtcggcacc
     1741 gccgcggtcg agcaccccac gctcgtcgac gacctctgcg cggcgcatcc gggccaggtc
     1801 gcggtcggtt tcgacgcccg gggccgcgac gtcgccaccc gcgcctggac cgcatcgtcg
     1861 ggccaggacc tcgtcgctct cgcccgacgg ttcgaggacc gcggcgtggc cgccctcatc
     1921 gtcaccgaga tcggccggga cggaactatg accggacccg acctcgacca gctggccgcc
     1981 gtcctcgatg ccaccgccct gccggtgatc gccagcggcg gtgtcggcca cctgggcgac
     2041 ctcgaggccc tcaccgcgct gagcgccggg ggccggcacc tcgccggggt catcgtcggg
     2101 cgggcgctct atgagggccg attcacgctg gctgaagccc tggcggtcgc ggccccatga
     2161 tcgagacgcc ggactcgggt acggtccgcg gaggccgacc acggagggga cctgcatgga
     2221 cgatgccgcg acgatcgacg aaggattcct tcggcgctac cgcgagctcc tcgacgccga
     2281 agacgaggcg ttcgacgagc tcgagcacgc ctacgaagac ggcgaacgag cccgcttcga
     2341 gcacgatgtc gaacgctggc ggatcgcggt ggaacgccgc cacaccttcc tcgaacgaca
     2401 cggcgtgacc ccgctcgtca ccagctgacc ggcccgtccc ggtcaggacg cgagccggcc
     2461 caccagaccg accgccagct ccacgagccg gtcggtgcga cccacgaaga agtgactggc
     2521 gcctcggacg acctcggtct cggtggccac ccacgacgcc gcggctgcct gcacctcggt
     2581 gggggagcgg aactcgtcgt gctcggcgag ggcgagcagc ttggggcgcc gatccccggc
     2641 gaggatgtcc aacccggtgg tgaaccgtag cggcggcgcg atcgcgtacc aggcgtcgat
     2701 ccggtcgtcg acgaccgaca acgccacgtc ggcgccgaat gaccaaccgg tcaggaccag
     2761 cggcgacgtc ggcgccaacc gcggcgcaag gtcgcggacc gcggctcgca cgtcggcctg
     2821 ctcgccccga ccctggtcgt gggtcccttc gctgccctcg acgccccgaa agttgaaccg
     2881 caagcacgtc acccccgccg gcggcaacgc cgcgaa
//
LOCUS       DATCCH010000191         2626 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_425366, whole genome shotgun sequence.
ACCESSION   DATCCH010000191 DATCCH010000000
VERSION     DATCCH010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425366"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1954
                     /locus_tag="VIJ44_03835"
     CDS             <1..1954
                     /locus_tag="VIJ44_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEY5165065.1"
                     /translation="PPDFPFVNRALKKRDLTEIIGDIVDRYSRADVARTLDHLKDLGF
                     EFATRAGLTISISDVRTPAKKAALLEKFEGEAEKVEQQYDRGILTDDERRQKEVEIWT
                     EATDEVRRAMEAELSAEKFNPVDMMVGSGARGNVMQVRQIAGMRGLVANPRGEIIPRP
                     IKSNFREGLSVLEYFISTHGARKGLADTALRTADSGYLTRRLVDVAQELIVREDDCES
                     ARGIWVENVTVDAEGLERRELETELLGRCLADDVPLTDGTSMPRNTEIREDEVAVLAT
                     DSSVERVRVRSVLTCDSIGGVCSHCYGTMLATGKMVDLGEAIGIIAAQSIGEPGTQLT
                     MRTFHTGGVAGEDITHGLPRVVELFEARTPKGASTLAESSGVVRLAENEKGERLVVVV
                     GDDGTEEEHVVSRRAQLAVREGQEVAAGARLVGEEQTPLDPKKLLEVKGIRETQQYLS
                     EEVQKVYREQGVSIHDKHVEVIVRQMLRRVAVSEPGDSPFLPGEKVDARFYAEVNRQL
                     VESGKQPAEGRPELMGITKASLATDSWLSAASFQETTRVLTEAAIEGKIDGLFGLKEN
                     VIIGKLIPAGTGMPDYNEIATNAPDYEPLPFYSSDASDVDLAEWLASSTSTAAAVEGE
                     PAGVAAGVLAAVPGTGEQLEGTGTEE"
     gene            2137..2508
                     /gene="rpsL"
                     /locus_tag="VIJ44_03840"
     CDS             2137..2508
                     /gene="rpsL"
                     /locus_tag="VIJ44_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020488528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEY5165066.1"
                     /translation="MPTIQQLVRKSRETKREKSKTPALKGSPQRRGVCTRVYTTTPKK
                     PNSALRKVARIRLTSGIEITAYIPGVGHNLQEHSIVLVRGGRVKDLPGVRYKVIRGAL
                     DASGVAGRRQARSRYGAKRDA"
     gene            2510..>2626
                     /locus_tag="VIJ44_03845"
     CDS             2510..>2626
                     /locus_tag="VIJ44_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEY5165067.1"
                     /translation="MPRKGPAPRRDLMPDPVYRSVVVTQLVNKVLQRGKRSLA"
BASE COUNT          457 a          917 c          890 g          362 t
ORIGIN      
        1 cccgcccgac ttcccgttcg tgaaccgtgc gttgaagaag cgggacctca ccgagatcat
       61 cggtgacatc gtcgaccggt actcgcgagc cgacgtcgcc cggaccctcg accacctcaa
      121 ggacctgggg ttcgagttcg ccacccgggc cggtctcacg atctcgatct ccgacgtgcg
      181 gaccccggcc aagaaggccg cgctgctcga gaaattcgaa ggcgaagccg agaaggtcga
      241 gcagcagtac gaccgcggga tcctgaccga cgacgaacgt cgtcagaagg aagtcgagat
      301 ctggaccgag gcgaccgacg aggtccgtcg ggcgatggag gccgagctct ccgccgagaa
      361 gttcaacccg gtcgacatga tggtcgggtc cggcgcgcgc ggcaacgtga tgcaggtccg
      421 ccagatcgcc ggcatgcgcg gcctggtggc caacccgcga ggcgagatca tccctcgtcc
      481 gatcaagtcc aacttccgcg aaggcctgtc ggtcctcgag tacttcatct cgacccacgg
      541 ggcccgcaag ggtctcgccg acaccgccct gcggaccgcc gactcgggct acctgacccg
      601 tcggctggtc gacgtcgccc aggagctcat cgtccgcgag gacgactgcg agagcgcccg
      661 cggcatctgg gtggagaacg tgaccgtcga cgctgagggc ctggaacgcc gtgagctcga
      721 aaccgagctg ctcggacgct gcctcgccga cgacgtgccc ctcaccgacg ggacgtcgat
      781 gccccgcaac accgagatcc gagaggacga ggtcgcggtc ctcgccaccg actcgtcggt
      841 cgagcgggtc cgggtgcggt ccgtgctcac ctgcgactcg atcggcggtg tctgctcgca
      901 ctgctacggg acgatgctcg ccaccggcaa gatggtcgac ctgggagagg cgatcgggat
      961 catcgcggcc cagtcgattg gtgagcccgg cacgcagctg accatgcgga ccttccacac
     1021 cggcggtgtc gccggagagg acatcaccca cggcctaccc cgggtcgtgg agctgttcga
     1081 ggcccgcaca ccgaaaggcg cgtcgaccct cgcggaatcg tcgggcgtcg tgcgcctggc
     1141 cgagaacgag aagggggagc ggctggtcgt ggtcgtcggc gacgacggca ccgaagagga
     1201 gcacgtcgtg tcccgccggg cccagctcgc ggtgcgcgaa ggccaggagg tcgccgccgg
     1261 tgcccgcttg gtcggtgagg agcagacccc gctcgacccg aagaagctgc tcgaggtgaa
     1321 gggaatccgc gagacccagc agtacctcag cgaagaggtc cagaaggtct accgggagca
     1381 gggcgtgtcg atccacgaca agcacgtcga ggtcatcgtc cgccagatgc tgcggcgcgt
     1441 cgccgtctcc gagccgggcg actcaccgtt cctgcccggc gagaaggtcg acgcccggtt
     1501 ctacgccgag gtgaaccgcc aacttgtcga gagcggcaag cagcccgccg aaggccgccc
     1561 cgagctcatg gggatcacga aggcctcgct cgccaccgat tcgtggctgt cggcggcgtc
     1621 gttccaggag accacgcggg tgctcaccga agccgcgatc gaaggcaaga tcgacggcct
     1681 cttcgggctc aaggagaacg tgatcatcgg caagctcatt ccggccggca ccgggatgcc
     1741 cgactacaac gagatcgcga cgaacgcccc cgactacgag ccgctgccct tctactcgag
     1801 cgacgcgtcg gacgtggacc tggccgaatg gctggccagc tcgacgagca ccgccgccgc
     1861 cgtcgagggt gagcccgccg gtgtcgccgc cggggtcctc gccgcggtcc cgggcacggg
     1921 cgagcagctc gagggcaccg gaactgagga gtagcccgtt gacccgaccg gggcgccgct
     1981 gtcgctcccc ggtcggtcga ccggcgcatt tggcgctcgc cgggcgcggg caaggtccgc
     2041 taatcttggc acggcttgga accgcccggc ctcgcggtcc tgagttggcc cgtccgaatt
     2101 cgacccccac gtgctgacgc gcgagaaggc agtcctgtgc ccaccatcca gcagctggtt
     2161 cgcaagagcc gcgagaccaa gcgagaaaag tcgaagaccc ccgcgctcaa gggctctccc
     2221 caacgccggg gcgtgtgcac tcgcgtctac acgacgaccc cgaagaagcc gaactccgcg
     2281 ctgcgcaagg tcgcccggat ccggctcacc tccgggatcg agatcactgc gtacatcccg
     2341 ggcgtggggc acaacctcca agagcactcg atcgtcctcg tccggggcgg acgggtgaag
     2401 gacctgcccg gggtccggta caaggtcatc cggggtgccc ttgacgccag cggtgtcgcc
     2461 gggcgtcgcc aggcccgcag ccgctatggc gcgaagcggg acgcctgaca tgccacgcaa
     2521 aggcccggct ccccgtcgcg acctcatgcc ggacccggtc taccggtccg tggtcgtcac
     2581 ccagctggtc aacaaggttc ttcaacgcgg caaacggtcg ctcgcc
//
LOCUS       DATCCH010000192         3154 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_560045, whole genome shotgun sequence.
ACCESSION   DATCCH010000192 DATCCH010000000
VERSION     DATCCH010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560045"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1053
                     /locus_tag="VIJ44_03850"
     CDS             <1..1053
                     /locus_tag="VIJ44_03850"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006545493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEY5165068.1"
                     /translation="SRNLDNEASDDSVRALVDAVTSRYDIPQRWYTLKAGLLGLPRLA
                     DYDRMASIASEDAAFGWSEARELVLDAYGSFSSELADIARRFFDESWIDAPVRPGKRP
                     GAFCAYTVPSLHPYVLLNWTGRRRDVLTLAHELGHGLHAYLARGQGVFHQSTPLTLAE
                     TASVFGETVTFGRLLGETTDPQARLALLAESLEGQIATVFRQIAMNRFEDRVHRARRE
                     QGELSVDGFGEHWIETQADMLGDSVELTDGYRTWWSYIPHFIGTPGYVYAYAYGQLLA
                     LSVYRQYEQLGAGFVPAYLELLAQGGSAPPEELGRIVGVDLADPGFWDGGLAIVEEQL
                     TAAEDAARATGRLPAT"
     gene            complement(1113..2798)
                     /locus_tag="VIJ44_03855"
     CDS             complement(1113..2798)
                     /locus_tag="VIJ44_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004002901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEY5165069.1"
                     /translation="MTSPVADLADRYVTRAAALDPIAATFEGLPGHDEELTDYSPDGI
                     SERLAHDRATLAELDRTAVTDEQDRVAAEVLRRYLDIGIDLAVTGEPLRALRILGSPV
                     SDVRMVFDLMPRETEEDWDRIAARAAAVPQALSSFQDALTEGAQEGLPAARRQVVACA
                     RQADTWGGQLAEDGRPFFLGLVDGFDAAGLDQPALRARLEQVTDRATAAYASLGRYLV
                     EEYLSYATERDAVGDERYARMARAYNGTVLNLTETYAWGWEELHRIEHAMASVANRIL
                     PGESIPAVIEHLERDPTRRISGADEFRAWLQALLDRTIAELDGVHFDLPEPVKRVEAM
                     IAPPGGAAAMYYTGPSEDFSRPGRTWYPTLGRTEFPLWGEVSICYHEGVPGHHLQIAM
                     VRYLTGRLNRFQRTLAGNSGHAEGWALYAERLMGELGYLDDPAFELGMLRAQAMRAVR
                     VVVDIGLHLELEIPTDESYHPGQRWTPDLALPFVIERSRFPADFMASEVDRYLGLPGQ
                     AISYKVGERVWLRGRDEARRRHGDAFDLKAFHAYALALGPMGLDQLDAELARF"
     gene            complement(2795..>3154)
                     /locus_tag="VIJ44_03860"
     CDS             complement(2795..>3154)
                     /locus_tag="VIJ44_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S15"
                     /protein_id="HEY5165070.1"
                     /translation="AATSTALEPQYTFRQAQARTMPKGRFTLVRVPLFPIAHAFRAGS
                     RVRLVIQAPGGNRPIWAFDALPATGNPHNDVAHSAVFASKLVLPVATGIRVPTPLPPC
                     PALRGEPCRSANPTGAP"
BASE COUNT          463 a         1035 c         1178 g          478 t
ORIGIN      
        1 agccgcaacc tcgacaacga ggcgagtgac gactcggtgc gggcactcgt cgacgcggtg
       61 acgagccggt atgacatccc gcagcggtgg tacacgctga aggcaggcct gctcgggttg
      121 ccccgcctcg ccgactacga ccggatggcg tcgatcgcgt ccgaagatgc cgcgttcggc
      181 tggagcgagg cccgcgagct ggtgctcgac gcgtacggct cgttctcgtc cgaactggcc
      241 gacatcgcac ggcggttctt cgacgagtcg tggatcgacg cgccagtgcg gcccgggaag
      301 cgcccgggtg cgttctgcgc gtacaccgtt ccgtcgttgc atccctacgt gctgctgaac
      361 tggaccggtc ggcgacgcga cgtcctcaca ctggcccacg agctgggcca cggactccac
      421 gcgtacctcg ctcgcggaca gggcgtgttc caccagtcga ccccgctcac cctggccgag
      481 acggcgtcgg tgttcggtga gaccgtgacg tttggtcgac tcctcggcga gacgacggat
      541 ccgcaggcgc ggctggcgtt gctcgccgag agcctggagg gtcagatcgc gaccgtgttc
      601 cgccagatcg ccatgaatcg cttcgaggat cgggtgcatc gggcgcggcg cgagcaaggc
      661 gagctgagcg tcgacggatt cggggagcat tggatcgaga cccaggcgga catgctgggc
      721 gactccgtcg agctcaccga cgggtaccgc acctggtggt catacatccc gcatttcatc
      781 gggacaccgg gttacgtgta cgcgtacgcc tacggtcagc tcctcgcact gtcggtgtat
      841 cgccagtatg agcaactagg cgcggggttc gtgcccgcct accttgagct cctcgcccaa
      901 ggcggctcgg cgccacccga agagctcgga cggatcgtcg gcgtcgacct cgcggacccg
      961 ggtttctggg acggggggct ggccatcgtc gaggagcagc tcacggcggc tgaggacgcc
     1021 gcacgggcga ccggacggct cccggctacc tagctttttg gcccggcagc ccgactcgcg
     1081 accgacccgc agggccgctc ggcgcgggtc gctcagaacc gggccagctc ggcgtcgagc
     1141 tgatcgagac ccatcgggcc gagggccagc gcgtacgcgt ggaacgcctt gaggtcgaag
     1201 gcgtcgccgt ggcggcggcg ggcctcgtct cgtccccgca gccagacccg ttccccgacc
     1261 ttgtacgaga tcgcttggcc cgggagcccc aggtaccggt cgacctcgct ggccatgaag
     1321 tcggcgggga agcggctccg ttcgatcacg aacggcaacg cgaggtccgg tgtccagcgt
     1381 tgcccagggt ggtacgactc gtcggttggg atctcgagct cgaggtgaag gccgatgtcg
     1441 acgacgacgc gcaccgcccg catcgcctgg gcgcgcaaca tgcccagctc gaaggcgggg
     1501 tcgtcgaggt agccgagctc gcccatgagc cgttcggcgt acagcgccca accttcggcg
     1561 tgaccggagt ttccggcgag ggtgcgctgg aagcggttca gccgcccggt caggtagcgg
     1621 accatcgcga tctggagatg atggccgggg acgccttcgt ggtagcagat gctcacctca
     1681 ccccagaggg gaaactcggt gcgccccaac gtcgggtacc aggttcggcc cggtcggctg
     1741 aagtcctccg acgggccggt gtagtacatc gcggccgcgc cgcccggcgg agcgatcatc
     1801 gcttcgacgc gcttcaccgg ctcggggaga tcgaagtgga ccccgtcgag ttcggcgatg
     1861 gtccgatcga gcagggcttg gagccaggcg cggaactcgt cggccccgct gatccggcgg
     1921 gtggggtccc gctcaaggtg ttcgatgacc gccgggatgc tctcgccggg aaggatccgg
     1981 ttggcgacgc tcgccatcgc gtgttcgatg cggtgcagtt cctcccaccc ccaggcgtac
     2041 gtctcggtga ggtttaggac ggtgccgttg tacgcgcgag ccatgcgggc gtaccgctcg
     2101 tcaccgaccg cgtctcgttc ggtggcgtac gacaggtact cctcgacgag atagcgcccg
     2161 agtgacgcgt acgcggcggt ggcccgatcc gtgacctgct cgaggcgagc gcgcagcgcc
     2221 ggctgatcga gcccggcggc gtcgaacccg tcgacgagac cgaggaagaa cggccgcccg
     2281 tcctcggcga gctggccgcc ccaggtgtcg gcttgacggg cgcaggccac gacctggcgc
     2341 cgcgccgcgg ggaggccctc ctgggcgcct tcggtgaggg catcctggaa cgacgagagg
     2401 gcctggggga ccgcggcggc ccgggcggcg atccgatccc agtcctcctc ggtctcgcga
     2461 ggcatgaggt caaagaccat gcggacgtcc gacaccggac tgccgaggat ccgcaaggcg
     2521 cggaggggct caccggtgac ggcgaggtcg atgccgatgt cgaggtaccg gcgcagcacc
     2581 tcggcggcga cccggtcctg ctcgtcggtg acggcggtgc ggtcgagctc ggcgagggtg
     2641 gcccggtcgt gcgccaggcg ctcactgatc ccgtcggggg agtagtcggt gagctcctcg
     2701 tcgtggccgg gaagaccttc gaacgtcgcc gcgatggggt cgagcgccgc ggcgcgagtc
     2761 acgtaccggt ccgcgaggtc ggcgacgggg ctggtcatgg agctccggtc gggttggcgc
     2821 tgcggcacgg ctcgccccgc agagccggac acggcgggag cggggtcggg acacggatcc
     2881 ccgtcgcgac cggcaagacc agctttgagg cgaacaccgc agagtgcgcg acgtcattgt
     2941 gggggttgcc ggtcgcgggg agggcgtcga aggcccagat cggccggttc ccgcccgggg
     3001 cttggatgac gaggcgaact cgggagccgg cccggaacgc gtgggcgatc gggaagagcg
     3061 ggactcgaac gagggtgaac cggcccttcg gcatggtgcg cgcctgggcc tggcggaacg
     3121 tgtactgcgg ctcgagcgcg gtcgaggtgg cggc
//
LOCUS       DATCCH010000193         2563 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_10465, whole genome shotgun sequence.
ACCESSION   DATCCH010000193 DATCCH010000000
VERSION     DATCCH010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10465"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..2257
                     /locus_tag="VIJ44_03865"
     CDS             29..2257
                     /locus_tag="VIJ44_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020034881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEY5165071.1"
                     /translation="MRLAETLVQVSRTVAAMETLDDVLATLVDLTTRETGADRGTLFL
                     NDPATGELYSRVAQGHRSREIRFPNDVGIAGHVFQTGMGLIVNDAYGHPKFNPEIDEQ
                     TGYRTERIVCAPIRTARGTVIGVAQVLNKQGGAFTDEELSLVEQMTTQAAVALMNSQY
                     VESMERKRSEELQFLELVSDITSELDLGALLQRIMGEATRLLKADRSTLFLNDERSGQ
                     LFARVAQGDSLDEIRFPNTVGIAGTVFTTGETINIPYAYADLRFNPGLDKQTGYFTRS
                     ILCVPVVNNDGETIGVTQVLNKRGGPFTAEDEQRLKAFTAQVSIALQNAKLFDDVQTM
                     KNYTEAMLESMSNGVLTLDEDGRIATCNAAGARILRSDEAKLVTQPASPVLSSANPLL
                     TDMVERVAQSQSPDLAMDLDLTVGDEDETVSVNATVLPLISADAAKLGTMVMLEDISG
                     EKRVRSTMARYMDPGLADQLVRRDEELLGGKNLEATIMFSDIRRFTTVAEELGPQGTV
                     ALLNDYFTIMVEAIQSQGGMLDKFIGDAIMAVFGLPVPHGDDTDRGVRAAISMITSLF
                     DWNQHRVSEGRMPIDMGIGLNTDLVVAGNIGSPKRMDFTIIGDGVNLASRLESACKQY
                     SARILISEHTFGRLKGVYRTREVDYVIVKGKTEPVGVYEILDYHTNETFPNLMDAVNC
                     FRDGIEKYRRGDWDQAVAAFGEALKANPDDQLSQTYIERCAQLVANPPAVWDGVWAMT
                     SK"
BASE COUNT          485 a          836 c          832 g          410 t
ORIGIN      
        1 cgactcgccg gccgatcaag cgaagcgcct gcgcttggcc gagaccttgg tccaggtgtc
       61 acgaaccgtc gccgcgatgg agaccttgga cgacgtcctg gccacgctgg tcgatctgac
      121 gacgcgcgag acgggcgccg atcgcggaac gttgttcctg aacgatcccg cgaccggcga
      181 gctctattcc cgggtggccc aaggccaccg gtcccgtgag atccgcttcc cgaatgacgt
      241 cgggatcgcc gggcacgtct ttcagaccgg catgggcctg atcgtcaacg atgcctacgg
      301 acatccgaag ttcaacccgg agatcgacga gcagaccggc taccggaccg agcgcatcgt
      361 ttgcgcgccg atccggaccg cccgaggcac ggtcatcggc gtcgctcagg tgttgaacaa
      421 gcagggtgga gcgttcaccg acgaggagct gtctctggtc gagcagatga cgacgcaagc
      481 cgcggttgcg ctcatgaaca gccagtacgt cgagagcatg gaacgcaagc gttccgagga
      541 acttcagttt ctcgaactgg tgtccgacat cacctcggag ctcgatctcg gtgcgctgct
      601 gcagcggatc atgggtgagg cgacccggct gctcaaggcc gaccgctcga cgctcttcct
      661 gaacgacgag cggtccggcc agttgttcgc acgcgtcgcc cagggcgact cgctcgacga
      721 gattcgcttc ccgaacaccg tcgggatcgc ggggacggtc ttcaccaccg gtgagacgat
      781 caacatcccg tacgcctacg cggacctgcg cttcaacccg ggcctcgaca agcagacggg
      841 gtatttcacc cgatccattc tctgtgtccc ggtggtcaac aacgacggcg agaccatcgg
      901 tgtgacccag gtgctcaaca agcgcggcgg cccattcacc gccgaagacg agcagcgact
      961 caaagcgttt accgcccaag tctcgattgc cctccaaaac gccaaactct tcgacgacgt
     1021 ccagacgatg aagaactaca ccgaagcgat gctcgagagc atgtcgaacg gcgtcttgac
     1081 cctcgacgag gacgggcgga tcgcgacctg caacgccgcc ggcgctcgca tcctgcgctc
     1141 cgacgaagcc aagctggtca cgcagccggc ctcgccggtc ctgagctccg cgaacccgct
     1201 gctcaccgac atggtcgagc gggtcgcgca aagccagagc cccgatttgg cgatggacct
     1261 ggacctcacg gtcggcgacg aggacgagac ggtctcggtc aacgccacgg tgctgcccct
     1321 gatcagcgcc gacgcggcga agctcgggac gatggtcatg ctcgaggaca tcagcggaga
     1381 gaagcgggtc cggtcgacga tggctcgcta catggacccc ggcctcgcgg accagctcgt
     1441 ccggcgggac gaggagctcc tcggcggtaa gaacctcgaa gccacgatca tgttctccga
     1501 cattcgccgg ttcacgacgg tggccgagga gctcggaccc cagggcaccg tcgccctcct
     1561 caacgactac ttcacgatca tggtcgaggc catccagagc caagggggga tgctcgacaa
     1621 gttcatcggt gacgcgatca tggccgtctt cgggttgccg gttccccacg gtgacgacac
     1681 ggaccgcggc gtccgggccg cgatctcgat gattacatcg ttgttcgact ggaaccaaca
     1741 tcgcgtctcc gaaggaagga tgccgatcga catggggatc gggctgaaca ccgacctcgt
     1801 ggtcgccggc aacatcggca gtccgaaacg catggacttc acgatcatcg gggatggcgt
     1861 caacctcgcc tcgcgtctcg agagcgcctg caagcaatac agcgcccgga tcctgatcag
     1921 cgagcacacc ttcggtcgcc tgaagggcgt ctaccggacc cgggaggtcg actacgtcat
     1981 cgtgaaggga aagaccgagc cggtcggcgt ctatgagatc ctcgactacc acacgaacga
     2041 gaccttcccg aacctgatgg acgcggtgaa ctgcttccgg gacggaatcg agaagtaccg
     2101 acgtggagac tgggatcagg ccgtggcggc gttcggggag gccctgaagg ccaaccccga
     2161 cgaccaactc tcccagacct atattgagcg ttgcgcgcaa ctggtggcga accctcccgc
     2221 ggtgtgggac ggcgtctggg cgatgacgtc gaaataggcc cggtctatgg ggcggtcatc
     2281 agggacctgt tcaccgtgac cgggcgatcc ggtcgctgcg aagtcacccg acccggcgcg
     2341 tccccgcccc gaagtgctca gcccgaaccg tggctagacg agtcgttgcc gagtgtggac
     2401 ggcccatcat gatggtcgtt gccgctgtgg cctccgttgt tgtccggagg cggggtggcg
     2461 tgatcgtcgc cgccctcaga gggtgccggc ggcggtgacg gctccgacgg cggtgtcgac
     2521 ccgccggacg gagcgggatc aggcgccggg gcgggcgacg acg
//
LOCUS       DATCCH010000194         2536 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_187471, whole genome shotgun sequence.
ACCESSION   DATCCH010000194 DATCCH010000000
VERSION     DATCCH010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187471"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VIJ44_03870"
     CDS             complement(<1..227)
                     /locus_tag="VIJ44_03870"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY5165072.1"
                     /translation="MARLVLVRHAEPDSSWGEHPDPGLSALGVRQAEEASRGLADFGR
                     AAIVTSPLRRARETAALLERHVGVEAGVDAR"
     gene            832..1044
                     /locus_tag="VIJ44_03875"
     CDS             832..1044
                     /locus_tag="VIJ44_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165073.1"
                     /translation="MHATVSGDDLWSIAATQLALASGRPISSLSNAEVAAYWQRVCDA
                     NQSHLQSGDVNLIYPGEQIALPPTGG"
     gene            complement(1089..>2536)
                     /locus_tag="VIJ44_03880"
     CDS             complement(1089..>2536)
                     /locus_tag="VIJ44_03880"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEY5165074.1"
                     /translation="FGRPVGQFQGVKHKCADMLGRVELARAATWDAARVAHEPDAGDL
                     TAASAASLALDAAFTNAKDCIQVLGGIGFTWEHDAHLYLKRASTLRSLAGPSSGWRVH
                     AARSALRGARRQLTVDLGPAAETIRAELRAFLDQVRDLPADQQRQRVADAGFLTPTWP
                     KPWGRDAKAIEQLVIDEEFRRARVRRPGISIGSWALPSVIVYGTRQQQERWIPPTLRG
                     ETTWCQLFSEPGAGSDLASLTTRATKAEGGWLVTGQKVWTSMAQTADWGILLARTDPD
                     LPKHDGISCFMVDMRHTDGLEIRPLRELTGESMFNEVFFDDAFLPDDCMIGRAGDGWR
                     AARTTLANERVFMGSGMTFGHGLEQILRMVTDGDTPDAVTLDEIGGIVATAHALACLG
                     FRLTLAALAGADPSGSEASVRKLLGVVHDQHVQEVGLALLGPRGAIAQGDGDTWSRGF
                     LFNRNLTIAGGTSEIQRNIVAERVLGLPRDP"
BASE COUNT          349 a          915 c          900 g          372 t
ORIGIN      
        1 aaccgggcgt cgacccccgc ttcgacgccg acgtgacgct cgagcagcgc ggcggtctcg
       61 cgggctcggc gtagtggcga cgtgacgatc gcggctcgcc cgaagtccgc gaggccacgg
      121 gaggcctcct cggcctgacg aactcccaac gccgagagac cgggatccgg atgctcgccc
      181 cacgacgagt ccggctcggc gtgacgcacg agcaccaacc gggccattgg atgcggaacc
      241 tactctcccg ccccatcgcc gaggcgagtc tcgagcaacg cgttcgcgtg acgcgcgtcg
      301 ggttcgcggg tcctgggagc acaaccgtgc ccgaccggag gtccgctcat gaaccgtgtc
      361 actcgcaagc tcctggcagt cgtctccatc gccggcggtg gcctcctcgg cgccgctccg
      421 gtcgcggccg cggccccgtc cgacccgacc ccggcgccgg cccctgctgc gaccagcgac
      481 gcgccgccga accccgcgcc ggcgcccgac ccgtcagtgc ggaccgggcg cgcacccgct
      541 ccaggcgaca cccgcgggtc gaccggggcc gcccccgccg ggcccgtcga ctcgcccagc
      601 gacccggtcc ggaccgggcg ggccactgac ctcgcaccga ccggggaccc gtcgagtcca
      661 gccgcgccgt cgcccgcccc gtcgtcgcct gctccggccc cgtcgacccc gtcgtcacca
      721 ccgcagtccc gagccaccgc cccgatcccc gccaccaacg acggcaacgc gtcggccgcg
      781 aagccaccga ctgccagcat tgcgaccggt ccgccgcccc cggatccgac ggtgcacgcc
      841 accgtcagcg gtgacgacct gtggagcatc gccgcgaccc agttggccct ggccagcggt
      901 cggccgatct cgtcgttgtc gaacgccgag gtagcggctt actggcagcg tgtctgcgac
      961 gcgaaccagt cccacctgca gtccggcgac gtcaacctca tctaccccgg cgagcagatc
     1021 gcgctgcccc cgaccggcgg ttgatccgtc cgcccaccgc cgcggtctcg ggcccggcgc
     1081 tcgcggcgtc aggggtcgcg gggcagaccg aggacgcgtt cggcgacgat gttgcgttgg
     1141 atctcgctcg tcccgccggc gatcgtgagg ttgcggttga agaggaaccc ccggctccag
     1201 gtgtctccgt cgccttgggc gatcgcgccg cgaggaccga ggagggcgag accgacttct
     1261 tggacgtgct ggtcgtgcac gacgccgagc agcttgcgaa cggaggcttc cgacccgctc
     1321 gggtcggcgc cggccagtgc ggccaaggtg aggcggaagc cgaggcaggc gagcgcatgg
     1381 gcggtcgcga cgatcccacc gatctcgtcg agggtgacgg cgtcgggggt gtcgccatcg
     1441 gtcaccatcc ggaggatctg ttccagaccg tgaccgaagg tcatgccact gcccatgaag
     1501 acccgttcgt tggcgagcgt ggtccgggcc gcccgccatc cgtcgccggc ccggccgatc
     1561 atgcagtcgt cggggaggaa ggcgtcgtcg aagaacacct cgttgaacat cgactcgccg
     1621 gtcagctcgc gcagcggccg gatctcgagc ccgtcggtgt ggcgcatgtc gaccatgaag
     1681 caggagatgc cgtcatgttt cgggaggtcg gggtcggtgc gggctaggag gatcccccag
     1741 tccgcggtct gggccatcga ggtccagacc ttctgtccgg tgacgagcca gccgccctcg
     1801 gccttggtcg ctcgggtcgt gaggctggcc agatccgatc cggcgccggg ctcgctgaac
     1861 agctgacacc aggtggtctc gccgcgaagg gtcggaggga tccagcgttc ctgctgctgg
     1921 cgggtcccgt agacgatgac cgacgggagc gcccagctac cgatactgat cccgggtcgc
     1981 cggacacggg cccggcggaa ctcctcgtcg atgacgagct gttcgatggc tttggcgtcg
     2041 cggccccacg gtttgggcca ggtcggcgtg aggaaaccgg catcggcgac tcgttggcgc
     2101 tgctggtcgg ctgggaggtc gcgcacctgg tcgaggaacg cgcgcagctc ggctcggatc
     2161 gtctcggctg ccgggccgag gtcgacggtg agttgacgtc gcgctcctct gagtgcgctg
     2221 cgggcggcgt gcacccgcca tccggatgac ggtccggcga ggctgcggag ggtcgaggcc
     2281 cgcttgagat acaggtgtgc gtcgtgttcc caggtgaacc cgatgccgcc gaggacctgg
     2341 atgcagtcct tggcgttggt gaaggccgcg tcgagggcaa gggacgccgc tgacgcggcg
     2401 gtgaggtcgc cggcgtccgg ttcgtgggcg accctggccg catcccacgt cgcggcgcgg
     2461 gcgagctcga ctcgtccgag catgtccgcg catttgtgtt tgacaccttg gaactgacca
     2521 accggtcgtc cgaact
//
LOCUS       DATCCH010000195         2730 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_478220, whole genome shotgun sequence.
ACCESSION   DATCCH010000195 DATCCH010000000
VERSION     DATCCH010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478220"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="VIJ44_03885"
     CDS             <1..721
                     /locus_tag="VIJ44_03885"
                     /inference="COORDINATES: protein motif:HMM:NF017774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEY5165075.1"
                     /translation="EEAEARLEREATRAREAERRLRDLRRQTPPGADPSGASPAPTTA
                     SVDVTAVVEAARVAERLAATLRDLAERGTAATASPVPATQPASGVARRARVSPPPGLV
                     TDTVAGLNAMLRTRGVVLIVDGYNISMRGWGDAAPSDQRDRLVAALAALHLRLRCEVV
                     VVFDGSDVEAVPARRRSGVRVVFSAADEEADPVVLREVDALPPAAPVLVASSDRWVRD
                     HAADLGAAVVSADTLLGVLRH"
     gene            825..1016
                     /locus_tag="VIJ44_03890"
     CDS             825..1016
                     /locus_tag="VIJ44_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018685730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165076.1"
                     /translation="MRIDCDDCVMQGTEVCDDCVVTFLVRRPARQAVVLDLDEARALR
                     RLAGAGLVPKLRHVTRREA"
     gene            complement(1043..2236)
                     /locus_tag="VIJ44_03895"
     CDS             complement(1043..2236)
                     /locus_tag="VIJ44_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013424434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5165077.1"
                     /translation="MPTAEDLILVSVDDHVVEPPTLFDAHLPDKLKEFAPRSVRKDDG
                     TDVWVYQGAEIPNIGLNAVAGRPPEEYNIEPTSYDMIRDGCYDIHERVQDMDRNGVLG
                     SMCFPSFVQFCGQLFSRSKDLDVGLQLLRAYNDWHIDEWCGTYPGRFIPLSIPPIWDP
                     QLMADEVRRVAKKGCHAVTFSENPFKLGWPHVFGDHWDPFFAACEDEGTVICLHIGSS
                     STTIEVAPGAPIDIMITLTPLNTMQAATDLLWSQVLRKFPNLQFALSEGGTGWIPYWL
                     ERIDYVYQQHRFWTHQDFGDKLPSEVALDHFTFCFISDRSGVEDRHRIGIDNITWECD
                     YPHSDSTWPHSPEAIAKQLEGVPEAEIAKITHENAMRIFRYDPFTQRPKERATVGVLR
                     AEASA"
     gene            2390..>2730
                     /locus_tag="VIJ44_03900"
     CDS             2390..>2730
                     /locus_tag="VIJ44_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013125402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HEY5165078.1"
                     /translation="MIFVHGWRHLAVTRGGPGIANWFEGLGLKPGQFHAWNVTLTELA
                     VGVVLVLGFLLLLVALQAPLIAAAGVLTNLLATGAAFGVAKWIFQDGVGHSLLGFQSQ
                     GFLDAWGPVFF"
BASE COUNT          398 a          903 c          963 g          466 t
ORIGIN      
        1 cgaggaggcc gaggcccgtc ttgaacgcga agcgacccgg gcccgggagg cggaacgacg
       61 gttacgcgat ctgcggcgcc aaacgccacc gggggctgac ccctccggcg cctctccggc
      121 gccgaccacc gccagtgtgg acgtcaccgc ggtcgtcgaa gcagcccgag tcgcggaacg
      181 actcgccgcg acgctgcgcg acctcgccga gcggggcacc gcggccacgg cgtcaccggt
      241 gcccgcgacg cagcccgcgt ccggcgtggc gcggcgcgcc cgggtgagtc ctccgcccgg
      301 gttggtcacc gacaccgttg ctggcctcaa cgccatgctc cgaactcggg gcgtggtcct
      361 catcgtggac gggtacaaca tctcgatgcg cggctggggc gacgcggcgc ccagcgacca
      421 acgggatcgg ctcgtggcgg ccctcgcggc gctgcatctt cggctgcggt gcgaagtcgt
      481 cgtcgtcttc gacggcagcg acgtcgaagc ggtcccggcc cgacgccgct ccggggtcag
      541 ggtcgtcttc tcggctgccg acgaagaggc cgacccggtc gtgctccggg aggtggatgc
      601 gcttcccccg gccgcgccgg tcctggtggc gtcgtcggat cggtgggtgc gtgaccacgc
      661 cgccgacctc ggagctgcgg tcgtctctgc cgacacgctc ctcggggtcc tgcgccacta
      721 acgccgagcc cgagcccgag cccgagaccg gctcgggccg gttcgacggg tgcggcgcct
      781 cgcggggcgt ttcggtgtca cacccccatg cgacgctgcg atccatgcgg atcgactgtg
      841 acgactgtgt gatgcagggc accgaggtgt gcgacgactg cgtcgtgacc ttcttggtgc
      901 ggcgaccggc ccggcaggcc gtggtcctcg acctcgacga agctcgggcg ctgcggcgcc
      961 tggccggtgc ggggctggtc cccaagctgc gccacgtcac ccgccgcgag gcgtgaccgg
     1021 agccgaaggg gctcgccgac gctcaggccg acgcttcggc gcggagcaca ccgacggtcg
     1081 cacgctcctt cggtcgctgc gtgaacgggt cgtaccggaa gatgcgcatc gcgttctcgt
     1141 gggtgatctt ggcgatctcg gcttccggaa ccccttcgag ctgcttggcg atcgcctccg
     1201 gcgagtgagg ccacgtcgag tcggaatgtg ggtagtcgca ttcccacgtg atgttgtcga
     1261 tcccgatccg gtgacggtcc tcgacgcccg accggtcgct gatgaagcag aaggtgaaat
     1321 ggtccagcgc gacttcgctc ggcagcttgt cgccgaagtc ctggtgggtc cagaagcggt
     1381 gctgctggta gacgtagtcg atccgctcca gccagtacgg gatccacccg gtgccgccct
     1441 cggagagcgc gaactggaga ttggggaact tgcgcaggac ctgggaccag agcagatcgg
     1501 tggcggcctg catcgtgttg agcggcgtca atgtgatcat gatgtcgatc ggcgcccccg
     1561 gcgcgacctc gatcgtcgtt gacgaggacc cgatgtgcag gcagatcacg gtgccttcgt
     1621 cttcacaggc cgcgaagaac ggatcccagt ggtcaccgaa aacgtggggc cagccgagct
     1681 tgaacgggtt ctcggagaac gtcaccgcgt ggcaaccctt cttcgccacc cggcgcacct
     1741 cgtcggccat gagctgagga tcccagatcg gcgggatcga caacggaatg aaccggcccg
     1801 ggtaggtgcc gcaccactcg tcgatgtgcc agtcgttgta ggcgcgtagg agctgcaggc
     1861 cgacatcgag atccttcgag cgcgagaaca gctggccgca gaactggacg aagctcggga
     1921 agcacatcga gccgagcacg ccgttgcggt ccatgtcttg gacgcgctca tggatgtcgt
     1981 agcagccgtc gcggatcatg tcgtacgacg tcggctcgat gttgtattcc tcaggcgggc
     2041 gcccggcgac cgcgttcagg ccgatgttcg ggatctcggc tccctggtac acccagacgt
     2101 cggtgccgtc gtcctttcgg acgctgcgag gagcgaactc tttgagcttg tcgggcaggt
     2161 gggcgtcgaa cagcgtgggc ggctcgacga catggtcgtc gacgctgacc aggatgaggt
     2221 cttcggcagt cggcatagcg gcggtagcgc tccctgatgg caggtacctg gcgtcaacgt
     2281 cagcctacgt cgctccgggt agcctcgctg ccgacggagg gggagaagcg tgcacgacat
     2341 cgatgtggcg aacttcgcac tggcactggc tcgagtgtgg ctggggatca tgatcttcgt
     2401 ccacggttgg cgtcacctgg ccgtcacccg cgggggaccg ggaatcgcca actggttcga
     2461 aggcctcgga ctcaagcccg gtcagttcca cgcctggaac gtcacgctca ccgagctcgc
     2521 cgtcggcgtc gtcctcgtgc tcggcttcct gctgttgctc gtggcgctcc aggcgccact
     2581 gatcgcggcc gcaggcgtgc tgacgaacct gctcgccacc ggtgcggcgt tcggcgtcgc
     2641 caagtggatc ttccaggacg gcgtggggca ctccctgctg gggttccaat cccagggctt
     2701 ccttgacgcc tggggaccgg tgttcttctt
//
LOCUS       DATCCH010000196         3766 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_52019, whole genome shotgun sequence.
ACCESSION   DATCCH010000196 DATCCH010000000
VERSION     DATCCH010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3766
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52019"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /gene="selA"
                     /locus_tag="VIJ44_03905"
     CDS             complement(<1..828)
                     /gene="selA"
                     /locus_tag="VIJ44_03905"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HEY5165079.1"
                     /translation="MTDPRRALPAVDRVLGSLDGLPRELLVHCARAALDEARRRASEG
                     DEVDADDVIDDARQRAAHLRHGLLQPVVNATGVLVHTNLGRAPLSGEALAAAANVGRG
                     YSNLEYRLDEGRRGSRHEHAGALLAQACGAEAGIVVNNNAAAVLLALGAVARGHEVIV
                     SRGELVEIGGGFRVPEIMAESGCRLVEVGTTNRTRIQDYTAQLGAETALLLKVHASNY
                     RMIGFTESVPVAALAELGPPVMVDAGSGLLDEATPWLPHRPAWLHDEPGVRQALEAGA
                     "
     gene            905..1669
                     /locus_tag="VIJ44_03910"
     CDS             905..1669
                     /locus_tag="VIJ44_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY5165080.1"
                     /translation="MEPRDAATVLLIRDAPDLQVFMLRRNLRSVFAAGAYVYPGGAVD
                     PDDSDDALDAGPDAGDAETGIPARFRVAAIREAFEEAGVLLARDATTGTPVDVGSLTA
                     ARQALHRGERSFAEIVSGLGVVLDTAALWPIGRFITPLGAPRRFDTWFFVAPAPDGHA
                     YRHDDGETVASLWTGPDAALERSRHGEFELIEPTRCTLALIARYSRVAPLFDAVRAAW
                     REPSPLEERYQDRGWVLRVGDDPRPQRDDRPRRTTA"
     gene            1669..2511
                     /locus_tag="VIJ44_03915"
     CDS             1669..2511
                     /locus_tag="VIJ44_03915"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY5165081.1"
                     /translation="MPDMTPGVPSALSPLVRRVVAPNPGPFTGPGTNTYLVGIDEVAV
                     IDPGPDVKSHIDAIVGASMKERVRWVVLTHTHPDHSPGTARLVKATRAEVVAFGKHRD
                     KDSDIVPDRVIGEGDTIEGSEWGLEVLHTPGHAPNHLCFLLEEERVLFTGDTVLGGMY
                     SVVSPARGGDMAVYVQTLERLRRLRLTRLAPGHGDVIDEPRARIDDYLAHRKQRGQQI
                     LRLVRKRGPVKVKDIVDTLYGSDGLPPELIEAAGWQVHAHLVELKADGKVTGTTARAA
                     WSAA"
     gene            complement(2524..>3766)
                     /locus_tag="VIJ44_03920"
     CDS             complement(2524..>3766)
                     /locus_tag="VIJ44_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009375206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HEY5165082.1"
                     /translation="GHIFKGDWFYCVMSNTSTPTPRATVPTEDDVRGMLAGVIDPELH
                     ASIVELDMVDEVRIADDGVITVRVALTTAGCPLRGQIQADVASKIRGLSGVTDVIVEY
                     GEMTPEQKTAAMQRARWNAREHAEPTEVPASTRVLAVASGKGGVGKSSVTVNLAVALA
                     ARGLTVGVLDADIWGFSVPRMLGVDGRLGGTEGKIHPNTRTIPNRSDPNGPDGVLKVV
                     SMGFLVDDEQTALMWRGLVLTRAVEQFLQDVRWGDMDYLLIDMPPGTGDIQMGLARML
                     PQAEMLVVTTPAQAAQKVAARVADMARRSYLKVTGVVENMSAFVAPDGTRYPLFGEGG
                     GEALATEIGAPLVAQIPLEPDVSTGADTGVPVALSSPDGVAGAAFHALAARVVEELLV
                     PVEMSSCTARILNLAAGGSPT"
BASE COUNT          554 a         1403 c         1283 g          526 t
ORIGIN      
        1 cgccccggcc tcgagggcct ggcgcacccc cggttcgtcg tggagccacg cgggtcgatg
       61 cgggagccac ggggtggcct cgtcgagcaa acccgaaccg gcatcgacca tgaccggcgg
      121 acccagctcg gcgagcgcgg cgaccggcac cgattcggtg aagccgatca tccggtagtt
      181 cgaggcgtgc accttgagga gcagcgcggt ctcggcgccc agctgggccg tgtagtcctg
      241 gatccgggtc cggttggtgg tgccgacctc gacgagccgg caacccgatt cggccatgat
      301 ctccggcacc cggaaaccac cgccgatctc gaccagctcg ccgcgagaca cgattacttc
      361 gtgccctcgg gcgaccgcgc cgagcgcgag cagcaccgcg gccgcgttgt tgttgaccac
      421 gatgccggct tcggcgccgc acgcctgggc gagcagcgca ccggcgtgct cgtgacgcga
      481 tccccgtcgc ccctcgtcca ggcggtactc gaggttcgag taaccgcgcc cgacgttcgc
      541 cgccgccgcg agggcctcgc cgctgagcgg cgctcgaccc agattggtgt gcaccaggac
      601 accggtggcg ttcaccaccg gctggaggag cccgtggcgg aggtgtgcgg cgcgctggcg
      661 ggcatcgtcg atgacgtcgt cggcgtcgac ctcgtccccc tcagacgcgc ggcggcgggc
      721 ctcgtcgagg gcggcacgcg cgcagtggac caacagctcg cgtggcagcc catcgagcga
      781 gccgaggacc cggtccaccg caggcagcgc gcgcctggga tcggtcacga cgccagcgta
      841 cgacgggccc cgattcgtgc gcagcggcca cccgcatcga atctgacccc cgtagaatga
      901 gcgggtggaa ccgcgggacg ctgccaccgt gctgctgatt cgggacgcgc ccgacctgca
      961 ggtgttcatg ctccgccgca acctccggtc cgtcttcgcc gccggggcgt acgtctaccc
     1021 cgggggtgcc gtcgaccctg acgacagcga cgacgccctc gacgccggcc ccgacgcggg
     1081 cgacgccgag acaggaatcc ccgcccggtt tcgagtcgcg gcgatccggg aggcattcga
     1141 ggaggccggc gtcctcctcg cccgcgacgc gacgaccggg acaccggtcg acgtcggatc
     1201 gttgaccgcg gcccgccagg cgctccaccg gggcgagcgc tccttcgccg agatcgtcag
     1261 cggtctcggc gtggtcctcg acaccgcggc gctgtggccg atcggccggt tcatcacccc
     1321 ccttggggct ccccgccgct tcgacacctg gttcttcgtg gcaccggcac cggacggcca
     1381 tgcctaccgt cacgacgacg gcgagaccgt cgcctcgctc tggaccggac cggacgccgc
     1441 gttggagcgg agccgccacg gcgagttcga gctcatcgag ccgacccggt gcacgctggc
     1501 gctcatcgcc cgctactcgc gggtcgcacc gctattcgac gccgtccggg ccgcctggcg
     1561 tgaaccgtcc ccactcgagg agcggtacca ggatcgaggc tgggtgctgc gagtcggaga
     1621 cgacccccgc cctcaacgcg acgatcgccc ccgaaggacc accgcctgat gcccgacatg
     1681 accccggggg tcccgagcgc cttgtcgccc ctggtgcgtc gcgtcgtcgc gcccaacccg
     1741 gggccgttca ccggtcccgg gacgaacacg tacctggtcg gcatcgacga agtggcggtc
     1801 atcgaccccg gcccggacgt gaaatcacac atcgacgcga tcgtcggcgc gtccatgaag
     1861 gagcgggtcc ggtgggtggt gctgacccac acccatcccg accactcccc cgggaccgcc
     1921 cggctggtga aggcgacgcg cgccgaggtg gtcgcgttcg gcaagcaccg cgacaaggac
     1981 agcgacatcg tccccgaccg cgtgatcggg gagggtgaca ccatcgaggg cagcgagtgg
     2041 ggcttggagg tcctccacac gcccggccac gcgcccaacc acctgtgctt cctgctggaa
     2101 gaggaacggg tgctcttcac cggcgacacc gtcctcggcg gcatgtactc cgtggtgagc
     2161 cccgcccggg gcggagacat ggcggtctac gtccagaccc tcgagcggct ccgtcggctg
     2221 cggctcaccc ggctggctcc cgggcacggc gacgtgatcg acgagccccg ggcccgcatc
     2281 gacgactacc tcgcgcaccg caagcagcgc ggccagcaga tcctgcgcct ggtccgaaag
     2341 cgcggcccgg tcaaggtcaa ggacatcgtc gacaccctct acgggtccga cgggctccct
     2401 cccgagctca tcgaagccgc ggggtggcag gtgcacgccc atctcgtcga gctcaaagcg
     2461 gacggcaagg taacgggcac caccgcgaga gccgcgtgga gcgccgcctg acccgaccgt
     2521 cggtcaggtc ggcgagccgc ccgcggcgag gttcaggatc cgggccgtgc agctgctcat
     2581 ctcgaccggg acgagcagct cctcgaccac ccgggcggcc agcgcatgga acgccgcgcc
     2641 cgccaccccg tcggggctcg acaacgcgac cggcaccccg gtgtcggcac cggtcgacac
     2701 gtcgggctcc aaggggatct gtgccaccag gggcgcgccg atctcggtgg ccagcgcttc
     2761 cccaccgccc tcaccgaaca gcgggtaacg cgtcccgtcg ggggcgacga acgcgctcat
     2821 gttctcgacg acccccgtga ccttgaggta cgagcggcgc gccatgtccg ccactcgagc
     2881 cgcgaccttc tgcgccgcct gcgccggcgt ggtcaccacg agcatctcgg cttgcgggag
     2941 catgcgcgcc agacccatct ggatgtctcc ggtcccgggc ggcatgtcga tcagcaggta
     3001 gtccatgtcg ccccaccgga cgtcttggag gaactgctcc acggcccggg tgagcaccag
     3061 gccgcgccac atgagcgcgg tctgctcgtc gtcgacgagg aaccccatcg agacgacctt
     3121 gagcaccccg tcgggcccgt tcgggtccga ccggttcggg atggtccggg tgttgggatg
     3181 gatcttgccc tcggtgccgc cgaggcggcc atcgacaccg agcatgcggg ggacgctgaa
     3241 cccccagatg tcggcgtcga gcacccccac ggtcaggcct cgggcggcga gcgcaaccgc
     3301 caggttcacc gtcacggacg acttgccgac cccacccttg ccgctcgcga cggccaagac
     3361 ccgtgtcgat gctgggacct cggtcggctc ggcgtgctcc cgggcgttcc accgggcccg
     3421 ctgcatcgcg gcggtcttct gctccggcgt catctccccg tactcgacga tcacgtcggt
     3481 cacgccggac aggccccgga tcttggaggc cacatcggcc tggatctggc cccggagcgg
     3541 gcatcccgcc gtggtcagcg ccacccggac ggtgatcacg ccgtcgtcgg cgatccggac
     3601 ctcgtcgacc atgtcgagct cgacgatgct ggcgtgcaac tcggggtcga tgaccccggc
     3661 gagcatgcct cggacgtcat cctcggtcgg gacggtggcg cgcggggttg gggtcgacgt
     3721 gttgctcatc acacaataaa accagtctcc cttgaaaata tgcccg
//
LOCUS       DATCCH010000197         2133 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_342943, whole genome shotgun sequence.
ACCESSION   DATCCH010000197 DATCCH010000000
VERSION     DATCCH010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342943"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="VIJ44_03925"
     CDS             complement(<1..808)
                     /locus_tag="VIJ44_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018240298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY5165083.1"
                     /translation="MSGRSVLVTGGSGYFGSVLVDDAVARGDRVRVLDRNPPEPREGV
                     EFVAGDIRDRPAVRDACEGIDIVFHNVAQVPLAKDRALFRSVNVVGTANLLVAARDAK
                     VAKVVHTSSSAVFGIPESNPVTEDTPPRPLEDYGRAKLEAEQLCREAVAAGLDVTIVR
                     PRTILGHGRLGIMAILFEFVADGAPVFVLNRGDNRYQFVHASDLADACLRAGDRAGAA
                     VYNVGATEFGTMRETLQALVDHAGTGSRVRSLPVGPTRAAMQSLAGLGLAP"
     gene            complement(805..1920)
                     /locus_tag="VIJ44_03930"
     CDS             complement(805..1920)
                     /locus_tag="VIJ44_03930"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5165084.1"
                     /translation="MAFALLESRPGGISTKQELVSALNATTVGPDLVPSPIVVVTGAA
                     GWLGQNLVRALAGPRARVRCLVHDADDAPLLENIAPTVETVVGDVRDPAAVDRLFETV
                     SAATVFHAAGVIHPQRAVRELFDVNVGGTQLVLDRARRAATSRFVHVSSNSPFGANPT
                     PTERFTEESPYNPYLGYGQSKFEAEQLVQRSSERGDLATVIVRPPWFYGPFQPVRQTR
                     FFAAVRRGRFPLPGAGLNRRSLVYTGNLVHGMLRAEVAAGAAGREYWIADAEPCELRA
                     VIETVRAALRAEGLTVSDRQPHLPAAAGVAAASLDRLLQARGRYVQAVHVLGELKDTI
                     ACDISRARAELGYDPSTSMLDGMRASVRFCLERGDAL"
     gene            2012..>2133
                     /locus_tag="VIJ44_03935"
     CDS             2012..>2133
                     /locus_tag="VIJ44_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005459433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEY5165085.1"
                     /translation="MSLTMSPEEREAFLAEVHVGVISVANDDRGPLTVPIWYTY"
BASE COUNT          333 a          791 c          736 g          273 t
ORIGIN      
        1 acggcgccag gccgagcccg gcgagacttt gcatcgcggc ccgggtcggt ccgaccggaa
       61 gcgaccggac ccgtgacccg gtgccggcat gatcgacgag ggcctgcagc gtctcgcgca
      121 tggtgccgaa ctcggtggcg cccacgttgt agacggcggc gccggcacgg tctccggctc
      181 gcaggcaagc gtcggcgagg tccgaggcgt ggacgaactg gtagcggttg tcgccccggt
      241 tcagcacgaa caccggcgcg ccgtcagcga cgaactcgaa caggatcgcc atgatcccca
      301 gccgcccatg cccgaggatg gtccggggcc gcacgatcgt cacatccagg cccgccgcga
      361 cggcttcacg gcacagctgc tccgcctcca gcttggcccg gccgtagtcc tccagcggac
      421 ggggtggcgt gtcctcggtc accgggttcg attccgggat cccgaacacc gcgctcgacg
      481 acgtgtgcac gaccttggcc accttcgcgt cccgcgccgc gacgagcagg ttcgcggtgc
      541 cgacgacgtt caccgaccgg aacagcgccc gatccttcgc caacggcacc tgcgcgacgt
      601 tgtgaaagac gatgtcgatg ccctcgcagg catcccggac cgccgggcgg tcccggatgt
      661 caccggccac gaactcgaca ccctccctcg gctccggcgg gttgcggtcg agcacccgca
      721 cccggtcacc acgcgcgacg gcgtcgtcga cgagcacgct gccgaagtac ccgctgcctc
      781 cggtcacgag caccgaccgc ccactcacag ggcgtcaccg cgctcgaggc agaagcgcac
      841 gctggcccgc atcccgtcga gcatcgacgt cgacgggtcg tagccgagct cggctcgggc
      901 ccgcgagatg tcacaggcga tcgtgtcctt cagctcgccg aggacatgca ccgcctggac
      961 gtaccggcct cgggcctgca ggaggcgatc gagggacgcc gccgcgaccc cggcggcggc
     1021 cggcaggtgc ggctggcggt ccgagacggt cagcccctct gcccggagcg cggcgcgaac
     1081 cgtctcgatc acggcccgca gctcgcacgg ctcggcgtcg gcgatccagt actcccgccc
     1141 cgcggcgcca gcggcgacct cggctcggag catcccgtgc accaggtttc ccgtgtacac
     1201 caacgagcga cggttcagcc ccgcaccggg cagcgggaac cggccccgac gcaccgcggc
     1261 gaagaaccgg gtctggcgca ccggctggaa cgggccgtag aaccacggcg gccggacgat
     1321 caccgtggcc agatcaccgc gctcggagct gcgctgcacc agctgctcgg cctcgaactt
     1381 cgactgcccg tagccgaggt acgggttgta cggagactcc tcggtgaacc gctcggtcgg
     1441 agtcgggttg gcgccgaacg gcgagttcga cgacacgtgc acgaaccggc tggttgccgc
     1501 ccggcgggcc cggtcgagga ccagctgggt tccgccgacg ttcacgtcga acagctcgcg
     1561 gacggcgcgc tgggggtgga tgacccccgc ggcgtggaag acggtggcgg cgctcacggt
     1621 ctcgaacagc cggtcgaccg ccgcgggatc gcgcacgtcg ccgaccacgg tctcgacggt
     1681 cggcgcgatg ttctcgagca ggggtgcgtc gtccgcgtcg tggaccaggc accgcacccg
     1741 agcgcgagga cccgcgaggg cgcgcaccag gttctggccc agccagcccg ccgcgccggt
     1801 gacgacgacg atcggtgacg gcaccaggtc cggaccgacg gtggtggcgt tcagggcact
     1861 cacgagctcc tgcttagtcg aaatgccacc gggcctagac tccagcagcg cgaacgccac
     1921 cgctgcgcct cgctgctcac tcccgcggcg ggcgccgcca ccacgttctc ggcgccacga
     1981 tgaccaaggg gacacggacc aaggggacac gatgtcgctc acgatgagcc ccgaggagcg
     2041 cgaggcgttc ctcgccgaag tccacgtcgg cgtcatcagc gtcgcgaacg acgaccgggg
     2101 acccttgacc gtcccgatct ggtacacgta cga
//
LOCUS       DATCCH010000198         3035 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_560528, whole genome shotgun sequence.
ACCESSION   DATCCH010000198 DATCCH010000000
VERSION     DATCCH010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560528"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="VIJ44_03940"
     CDS             <1..515
                     /locus_tag="VIJ44_03940"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5165086.1"
                     /translation="TRSEFGRIDVLVNNAMAPTRAPFLDSTATEWDESMRVNVRSLYV
                     TCRAVVPAMADDGGGSIINVSSGAADPNVTGLPPGFLTYSVAKAALERFSTGLAGEVA
                     SLGVAVNALRPGAVKTETAVHELGEDSDWTGWTTPDAVVPAVVFLAAQRGDGVTGRVL
                     ESTQFGIMWP"
     gene            622..801
                     /locus_tag="VIJ44_03945"
     CDS             622..801
                     /locus_tag="VIJ44_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165087.1"
                     /translation="MPDIVQCPGCHLPVDIARPPDVAFQVQRGQNSQGREIIMIDVGL
                     VTVHRCTLCADGEWR"
     gene            complement(847..1839)
                     /locus_tag="VIJ44_03950"
     CDS             complement(847..1839)
                     /locus_tag="VIJ44_03950"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HEY5165088.1"
                     /translation="MRALRVHRHGEPEQALALEDIPVPVPGPGQVTMRVGAAALGLQD
                     LLLVRGSYQLKPSLPFTPGLEAAGTVIAVGDAVDTAWIGQRVVGVPALPDGGFAEDAV
                     LASSNLYPLRDDVSDVDAAATHIAFTTAHVGLHRRAGLQPGETLLVHAGAGGTGSAAI
                     QLGVLAGARVIATAGSPERTAICRDLGASVAIDYRHEDFETVVRDLTGGRGADVIFDP
                     VGGSVFERSRHCIASEGRLLLIGFASGEPQTIKANGVLLGNYSVMGVYMGAYSRGDAD
                     HELLRGVHDELMSWLSVGDVRAVVDREVDLRGVAEALGQLRDRKVIGRIVVRLA"
     gene            complement(1841..>3035)
                     /locus_tag="VIJ44_03955"
     CDS             complement(1841..>3035)
                     /locus_tag="VIJ44_03955"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY5165089.1"
                     /translation="PAVSAEEPLTGTRIVDLTTFLSGPVAARTLADLGADVIRVEPPQ
                     GDPTRAGTAMHRGDPPSAFYVALHRDRRSIVLDLKTDAGRDVLYDLVAASDVLLENYR
                     PGVTSRLGVSYDDVRPFNPRLVYCTITGFGPDGPYSQLPATDGAVQAFSGVLELTGEQ
                     DEFGLPVPIPIADLVAGATAAQGVLAALVARQRTDAGTHLDVSMVETLLGWLLVGDRE
                     RTLAPPTTLVVEGDDGIPLLVQTSLHFQPRLAELLAKVPGCAELLADPRFASRESRQE
                     HRDEYERLVRRAFASHPSDAWLEALQTIGIPASRIQPVDVALAHPQLDYREATATLDI
                     PGLGRQAVLASPFRFDGRRKAETTPPPTLGEHTGVVLRTILGYDDDTVTDLTRRGAFG
                     TTAVD"
BASE COUNT          466 a         1046 c         1081 g          442 t
ORIGIN      
        1 ccacccggtc agagttcggg cgcatcgacg tgctggtcaa caacgcgatg gctccgaccc
       61 gggccccttt cctcgacagc accgccaccg agtgggacga atcgatgcgt gtcaacgtcc
      121 ggagcctcta cgtcacctgc cgggccgtgg tgccggccat ggccgacgac ggcggcggga
      181 gcatcatcaa cgtctcgtcc ggtgccgcgg atcccaacgt caccggcctg ccgcccgggt
      241 tcctcaccta ctccgtcgcg aaagccgcgc tcgagcggtt cagcaccggc ctcgcgggcg
      301 aggtcgcttc gctcggcgtc gcggtcaatg ccctgcgtcc cggcgccgtc aagaccgaga
      361 ccgcggtgca cgagctcggc gaggacagcg actggaccgg atggaccacc cccgacgcgg
      421 tcgtccccgc agtggtgttc ttggccgctc agcgcggtga cggtgtcacc gggcgtgttt
      481 tggagtcgac gcagttcggg atcatgtggc cgtgatgacg acgcgccgag cgtcgggtgc
      541 gcggcggcac ggacccttct aaggttgacg tgccgaccgc gccatcctcg gctcgtcagc
      601 tgagtacgga ggagaacctg catgcctgac atcgtgcagt gtccggggtg ccaccttccg
      661 gtcgacatcg cccgcccacc tgacgtcgcg ttccaggtgc agcgaggcca gaacagtcag
      721 ggccgcgaga tcatcatgat cgacgtcggg ctcgtcacgg tgcaccgctg caccctctgc
      781 gcggacgggg agtggcgcta gtcggagcgt cggttcccgg attatgcgag gaccgcatcg
      841 gtgcggtcac gcgagacgca cgacgattcg accgatcacc ttccggtcgc ggagctggcc
      901 cagggcctcc gcaacgccac gcagatccac ctcacgatcg acgacggccc ggacgtctcc
      961 gacgctcaac cagctcatca gctcgtcgtg gacgccccga agcagctcgt ggtcggcgtc
     1021 gccgcggcta tacgccccca tgtacacccc catcaccgag tagttgccca ggaggacccc
     1081 gttggccttg atggtctggg gctcgccgga ggcgaagccg atgagcaaca agcgtccctc
     1141 cgacgcgatg cagtgacgcg accgctcgaa gaccgaacct ccgaccgggt cgaagatcac
     1201 gtccgcgcca cgacctccgg tgaggtcgcg caccaccgtc tcgaagtcct cgtggcggta
     1261 gtcgatggcc acggacgcgc cgaggtcgcg gcagatcgcg gtccgctccg gggagccggc
     1321 ggtggcgatc acccgggcgc ccgccagcac accgagctgg atcgccgccg atccggtccc
     1381 gccggcgccg gcgtggacca gcagcgtctc accgggttgt agcccagctc gtcggtgcag
     1441 cccaacgtgc gcggtggtga acgcgatgtg ggtcgcggcg gcgtcgacgt cgctcacgtc
     1501 gtcgcgaagt gggtagaggt tgctggacgc caagacggca tcctcggcga aaccgccgtc
     1561 cgggagtgcg ggaaccccca ccacccgctg gccgatccac gccgtgtcga ccgcatcgcc
     1621 gacggcgatg acggtccccg cggcttcgag accgggggtg aacggcaggg acggcttcag
     1681 ctggtacgag ccccggacca gcagcagatc ctggaggccg agcgcggcgg cgccgacccg
     1741 catcgtcacc tgacctgggc cgggaacggg gaccgggatg tcctccaggg cgagcgcctg
     1801 ctccggctcg ccgtgacggt ggacccgcag cgcccgcatc tcagtcgacg gctgtggtgc
     1861 cgaacgcgcc gcgccgcgtg aggtccgtca ccgtgtcgtc gtcgtatccc aggattgtgc
     1921 ggagcacgac accggtgtgc tcgccgagcg tcggcggcgg ggtggtctcg gccttgcggc
     1981 gcccgtcgaa gcggaacggg gaggcgagca ccgcttggcg accgaggccg gggatgtcga
     2041 gcgtggcggt cgcctcccgg tagtcgagct gggggtgggc cagcgcgacg tccacgggct
     2101 ggattctcga agcggggatg ccgatggtct gcagcgcctc gagccaggcg tcgctcgggt
     2161 gtgacgcgaa cgcccgacgg accaaccgct cgtactcgtc gcggtgctct tggcgactct
     2221 cgcgtgatgc gaagcgagga tcggcgagaa gttcggcgca gcccgggacc ttcgctagga
     2281 gctcggccag gcgaggctgg aaatggaggc tggtctgtac cagcaagggg atgccgtcgt
     2341 cgccttccac cacgagcgtc gtgggtggcg cgagggtgcg ttcccggtcc ccgaccagca
     2401 gccacccgag caaggtctcg accatgctca cgtccaggtg cgtgcccgcg tcggtgcgct
     2461 gtcgggccac gagcgcggcg agcaccccct gcgcggcggt cgcgccggcg acgagatcgg
     2521 cgatcgggat cgggaccggc agaccgaact cgtcctgctc gccggtgagc tcgagcactc
     2581 cactgaacgc ctgtaccgcg ccgtcggtgg cgggcagctg cgagtacggg ccatcgggac
     2641 caaatccggt gatcgtgcag tacacgagac gggggttgaa cggtcgaacg tcgtcgtagc
     2701 tgacacccag gcgcgacgtg acgccagggc gatagttctc gagcagcaca tcggaggccg
     2761 cgacgaggtc gtacaagacg tcccggccgg cgtcggtctt gaggtcgagg acgatgctgc
     2821 gccgatcccg gtgcagcgcg acatagaacg ctgacggggg atcaccgcgg tgcatggccg
     2881 tgcccgcccg tgtcggatca ccttgcggtg gctcgacgcg gatcacatcg gcgcccagat
     2941 cggcgagcgt ccgcgccgcg accggacccg acaggaacgt ggtcaggtcc acgatgcggg
     3001 tcccggtcag cggctcctcc gccgacaccg ccggg
//
LOCUS       DATCCH010000199         2389 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_560585, whole genome shotgun sequence.
ACCESSION   DATCCH010000199 DATCCH010000000
VERSION     DATCCH010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560585"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="VIJ44_03960"
     CDS             complement(<1..430)
                     /locus_tag="VIJ44_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEY5165090.1"
                     /translation="MVEPVCSVYPLPSTAAPDRNTDPVVNMEPDQVRTFLSHGTRTAK
                     LATVTADGRPHVVPVWFVLDNDDLIFTTHETSQKAQSLRRDPRIACCVDDERARYSYV
                     MASGSATLSTNFEDLLRWTIRIGGRYMGEDRAEEFGRRNGL"
     gene            complement(758..845)
                     /locus_tag="VIJ44_03965"
     tRNA            complement(758..845)
                     /locus_tag="VIJ44_03965"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(809..811),aa:Ser,seq:gga)
     gene            890..1156
                     /gene="ffs"
                     /locus_tag="VIJ44_03970"
     ncRNA           890..1156
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VIJ44_03970"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            1209..>2389
                     /gene="dnaX"
                     /locus_tag="VIJ44_03975"
     CDS             1209..>2389
                     /gene="dnaX"
                     /locus_tag="VIJ44_03975"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEY5165091.1"
                     /translation="MAYQALYRKYRPQRFGEVVGQDHVTVALRNAVRDGRVGHAYLFS
                     GPRGTGKTTTARILAKALNCTNLRDDGEPCGECEHCVAIAAGTFLDLFEIDAASKRKV
                     DDARELREQLGHKGFGGQRTVYILDEVHMLTKESANTLLKSIEEPPEHVVFVLATTNP
                     EEVLPTIRSRTQHYQFTLLTPDELTAHLADILGRENVDFDPEALDVLAAAAGGSARDA
                     LSLLDQALARSDGRLDTATVTSVFGSTPFALRTQVLQTIADEDTAGALVALSALLDAG
                     HEPRRVAEDLLAAARDTFLLAAGGDRVTVSAPAADQARLRQLAERLGNAALVRIVETL
                     GQAIVDMRGVDAADPRLVLEVALVRLSRRDAGPPLQTVVERIERLERALAGGASSPPP
                     PA"
BASE COUNT          373 a          821 c          847 g          348 t
ORIGIN      
        1 gcaggccgtt gcgccgaccg aactcctcgg ctcggtcttc gcccatataa cgcccaccga
       61 ttcggatcgt ccaccgcagc agatcctcga aatttgtgct gagcgtcgcc gaaccggacg
      121 ccatcacgta cgagtagcgc gctcgttcgt cgtcgacgca gcacgcgatg cgcggatcgc
      181 ggcgcagcga ctgggccttc tgggaggttt cgtgagtggt gaagatgagg tcgtcgttgt
      241 cgagcacgaa ccagaccggg acgacatgcg gacgaccgtc ggcggtgacg gtcgcgagct
      301 tggcggtgcg ggtcccgtgg ctgaggaagg tccgcacctg gtccggttcc atgttgacca
      361 ccgggtcagt attgcggtcc ggtgccgcgg tcgacggaag gggatagacg gagcagaccg
      421 gttcgaccac gtaccccggg gggtcgggag gtgcttgatg atccgctcgg tccgtatgac
      481 ctggtcgggc cgatgggctt aggcggcgat gggggccagc ccgccgggcg tggtgtcgcc
      541 ggcgatgacg acgcgatcgc gtctgagctg cgtggctctt gaagagcgcc gagtcggccg
      601 ccccgccggc tcgcggacgt tggtgacgag gcgagccacg gtgccgcatc aggagggagc
      661 ggcggtggct acgggctggc cacatcgagg agcgcgacac caccgctttg gcgcggccaa
      721 acgcggcctt gcaccgatgg aagacgcggc gggccggtgg cggagggagt gggattcgaa
      781 cccacggtga gttgccccac acacgctctc caggcgtgcc gattcgtccg ctctcgcatc
      841 cctccgaagt tcggcatgct acgcaacggg actaacctca ctgcgcggac cgtgctaggc
      901 ggggagctcg tggtgccctg taccccaaat ccacgcacgg ggggctgaag tcccgcccga
      961 ggcttgggcc gctgttcgat cggacccgtt cgaccggcgt tgagggtcgg gtcctgcgca
     1021 acggaacccc gcgaaccgag tcaggtccgg aaggaagcag ctctcagcgg atcgtcccgt
     1081 gtgccgcagg taatcctgac cggagccgac ggcgggcacc gcacggacgg cgacgaggtc
     1141 gacggcgggt gcacggtccg cttcgctcac ccgtcgggac ggcggctcga cgtcgataca
     1201 gtccgtgcgt ggcctatcag gcgctgtacc gcaagtaccg accgcaacgg ttcggggagg
     1261 tggtcggcca ggaccatgtg accgtggcgt tgcgcaacgc ggtacgcgat gggcgggtcg
     1321 ggcacgccta cctgttctcc ggacctcgcg gcaccgggaa gacgaccacc gcccgaatct
     1381 tggccaaggc cctgaactgc accaacctgc gcgacgacgg cgagccgtgt ggcgagtgcg
     1441 agcattgcgt ggcgatcgcc gccgggacct tcctcgacct gttcgagatc gacgccgcgt
     1501 cgaaacggaa ggtggacgac gcccgcgagc tgcgcgagca gctcggccac aagggcttcg
     1561 gtgggcagcg gaccgtctac atcctcgacg aggtccacat gctcacgaag gagtcggcga
     1621 acacgctgct gaagagcatc gaggagccac ccgagcacgt ggtgttcgtc ctggccacga
     1681 ccaaccccga agaggtgctg ccgaccatcc ggtcgcgcac ccagcactac cagttcacgc
     1741 tcctcacgcc ggacgagctc accgcgcacc tcgccgacat cctcggtcgc gagaacgtcg
     1801 acttcgaccc cgaagcgctc gacgtgctcg ccgccgccgc gggagggtca gcccgagacg
     1861 ccttgtcgct cctcgaccag gcgctcgccc gctccgacgg acgactcgac acggccacgg
     1921 tcacgtcggt cttcggctcc acgccattcg cgctgcgcac ccaggtcctc cagacgatcg
     1981 ccgacgagga caccgccggc gcgcttgtcg cgctcagcgc gctcctcgac gccggccacg
     2041 agccccgccg ggtcgccgaa gacctgctcg ccgcggcccg cgacaccttc ctcctcgccg
     2101 cgggcggcga ccgggtgacg gtcagcgctc cggctgccga ccaggcccgg ctacgccagc
     2161 tggcggagcg gttgggcaac gcggcgctgg ttcggatcgt cgagacgttg gggcaggcga
     2221 tcgtcgacat gcgcggcgtg gacgccgccg acccccggct cgtcctcgag gtggccttgg
     2281 tgcggctcag ccgccgagac gcgggaccgc cgctgcagac ggtcgtggaa cgaatcgagc
     2341 gtctcgagcg cgccctggcg ggcggggcga gcagcccgcc accgccggc
//
LOCUS       DATCCH010000200         3239 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_478596, whole genome shotgun sequence.
ACCESSION   DATCCH010000200 DATCCH010000000
VERSION     DATCCH010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478596"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="VIJ44_03980"
     CDS             complement(<1..705)
                     /locus_tag="VIJ44_03980"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF006140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEY5165092.1"
                     /translation="MEFEAIRYEVDGPVATITLNRPDTANAQNSQMLDELDAALDLAD
                     RDDAVRVVVVAGSGKHFSAGHDLKEILSGGDEWARLRATPEGKWRHEQVMYFDRCVRL
                     RDFRKPTIASVQGACAAAGLMLACMCDLIVAADDARFSNPVARMTGVGVELLVEPWEL
                     GPRKAKEFLLCAETLNAADAERFGLVNRVAATDQLASATRAMADEVALVPPVTAQAIK
                     ASINQMLDLSGQRDSWR"
     gene            complement(780..1973)
                     /locus_tag="VIJ44_03985"
     CDS             complement(780..1973)
                     /locus_tag="VIJ44_03985"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY5165093.1"
                     /translation="MNLTPTADEERLRHDVRAWLRDHLPWEYGRGLPPRFDDLAEEVT
                     FLREWQARLAADRWVGVAWPAEYGGRGAGPVEHFIVTEELARARAPELVGRIGINLVG
                     PTLLAHGTEEQRARWLPTILPAEELWCQLFSEPGAGSDLASLTTRATATAGGWVLNGQ
                     KVWTSYAQFADWGLCLARTDPDVAKQRGISAFAVDMRTAGLEVRPLRQITDETEFNEV
                     FFSDVFVPEDRLVGPHNEGWRVANSTLTHERGINPRQLVIHLQLVDELLRLGLLEGAW
                     DDDRLRPRLAEAAVEVRLFQLANWRTLSRVAKGRDPGPEGSAMKLAWSEMSKRLHDTV
                     MAVLGSAAPLWHDAADNPGDGAWQRSWLYYQASSIWAGTNEIQRNILSERVLGLPREP
                     ARSAR"
     gene            complement(1970..3226)
                     /locus_tag="VIJ44_03990"
     CDS             complement(1970..3226)
                     /locus_tag="VIJ44_03990"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEY5165094.1"
                     /translation="MELPVVRGTENELGVDIAKLRAQSGLVTLDYGLVNTGSCESAIT
                     FIDGDQGILRYRGIPIEQLVSGHSPSFLETAYLLIYGELPTRAQRDEFSRSIRKHTLL
                     HEEAKRFFDGFPHDAHPMGALSSVVSALSTFYPDSADPHDPDQVQLAIVRLIAKLPTI
                     AAYSYKKSIGQPFLYPNNQLDLIENFLTMMFAVPSEPYEVSPTVVHALKILLILHADH
                     EQNCSTSTVRLVGSSEANLFASVAAGINALWGPLHGGANQAVIEMLGHIRADGNDIAK
                     YVSKAKDPNDPFRLSGFGHRVYRNYDPRARIIKDQAHTVIEELGKMDDLLELALRLED
                     VAISDDYFIERKLYPNVDFYSGLLYRAMGFPTNMFPVLFALGRLPGWIAHWKEMMESP
                     KTKIGRPRQIYVGVTERDYVPIDERG"
BASE COUNT          505 a         1033 c         1150 g          551 t
ORIGIN      
        1 ccgccacgag tcacgttggc ccgacagatc cagcatctgg ttgatcgacg ccttgatcgc
       61 ctgggccgtg accggcggga ccagcgccac ttcgtcagcc atggcccgag tcgccgacgc
      121 gagctggtcg gtggccgcga cccggttgac gagcccgaac cgttcggcgt cggcggcgtt
      181 gagcgtctcc gcgcagagca ggaactcctt ggccttgcgg gggccgagct cccacggctc
      241 gacgagaagt tcgacgccga cgccggtcat ccgggcgacc gggttcgaga accgggcgtc
      301 gtcggcggcg acgatgaggt cgcacatgca cgccagcatg aggccggcgg cggcacacgc
      361 gccctggacc gacgcgatcg tgggcttgcg gaagtcgcgg agccggacgc aacgatcgaa
      421 gtacatgacc tgctcgtgtc gccactttcc ttccggtgtg gcgcgcagac gcgcccattc
      481 gtcgccgccg ctcaggatct ctttgaggtc gtgtccggcg gagaagtgct tgcctgagcc
      541 cgccaccacc acgacgcgga cggcgtcgtc gcggtcggcg aggtcgaggg ccgcgtcgag
      601 ctcgtcgagc atctggctgt tctgcgcgtt cgcggtgtcg gggcggttca gtgtgatcgt
      661 cgcgaccgga ccgtcgacct cgtagcggat ggcttcgaac tccacgacgc catcatgcca
      721 gcgacccgag acccggggct gctctcgagg cccagtgcca cctgccggag cgtggccggt
      781 cagcgggccg aacgtgccgg ttctcgtggc aggccgagga cgcgctcgct gaggatgttg
      841 cgttggatct cgttggtgcc ggcccagatc gacgatgcct ggtagtacag ccatgagcgt
      901 tgccaggcgc cgtcacccgg gttgtcggcg gcgtcatgcc acaacggcgc ggcggacccg
      961 agcaccgcca tcaccgtgtc gtgcaaccgc ttgctcatct cgctccaggc cagcttcatc
     1021 gcgcttccct cgggtcccgg gtcgcgaccc ttcgccaccc gggacagggt ccgccagttg
     1081 gcgagctgga agagccgcac ctcgacggcg gcttcggcga ggcgcggtcg gagccggtca
     1141 tcgtcccagg cgccctcgag cagaccgagc cgcagcagct cgtcgacgag ctggagatgg
     1201 atcacgagct ggcgggggtt gatccctcgc tcatgggtga gggtcgagtt cgcgacgcgc
     1261 cacccctcgt tgtgtgggcc caccagccgg tcctcgggga cgaagacgtc gctgaagaac
     1321 acctcgttga actcggtctc gtcggtgatc tggcgcaggg gccgcacctc gaggcccgcg
     1381 gtgcgcatgt cgaccgcgaa cgccgagatg ccacgctgct tcgcgacgtc ggggtcggtg
     1441 cgggccaggc acaggcccca gtcagcgaac tgcgcgtagc tggtccagac tttttggccg
     1501 ttgaggaccc acccgccggc ggtggcggtg gcgcgggtcg tgagggacgc cagatcgctc
     1561 ccggcccccg gctcgctgaa cagctggcac cacagctcct cggccgggag gatggtcggt
     1621 agccatcggg ctcgctgttc ctccgtgccg tgcgccaaca gcgtgggacc caccaggttg
     1681 attccgatcc gtccgacgag ctccggcgcg cgcgcacggg cgagctcctc ggtgacgatg
     1741 aaatgctcga ccggacccgc gccgcggccg ccgtactccg cgggccaggc gacaccgacc
     1801 cagcgatcag ccgcgaggcg ggcctgccat tcacgaagaa aggtgacttc ctcggcgaga
     1861 tcgtcgaacc gtggggggag tcctcgcccg tactcccaag gcaggtggtc gcgcagccag
     1921 gcccggacgt cgtggcggag gcgttcttcg tccgcggtgg gggtgaggtt caaccgcgtt
     1981 cgtcgatcgg gacgtagtcc cgctcggtca ccccgacgta gatctgacgg ggccgcccga
     2041 tcttggtctt ggggctctcc atcatctctt tccaatgcgc gatccagccc ggcagccgac
     2101 cgagcgcgaa caggacgggg aacatgttgg tggggaaccc catcgcgcgg tagaggagac
     2161 cggagtagaa gtcgacgttg gggtacagct tgcgctcgat gaagtagtcg tcggagatcg
     2221 cgacgtcctc gaggcggagc gcgagctcaa ggagatcgtc catcttcccg agctcctcga
     2281 tgacggtgtg ggcctgatcc ttgatgatcc gtgcccgagg gtcgtagttc cgatagacgc
     2341 ggtgtccgaa cccggaaagc cggaacgggt cgttggggtc cttggctttc gagacgtact
     2401 tggcgatgtc gttcccgtcg gctcggatgt ggccgagcat ctcgatgacc gcctggttgg
     2461 cgcccccgtg aagggggccc cagagcgcgt tgatgccggc ggcgacggac gcgaagaggt
     2521 tcgcctcgct ggagccgacg agacggaccg tcgacgtgga gcaattttgc tcgtgatccg
     2581 cgtggaggat gagcaggatc ttgagcgcgt ggacgacggt ggggctcacc tcgtacggct
     2641 cggatgggac ggcgaacatc atggtcaaga agttctcgat caggtcgagc tggttgttcg
     2701 ggtacaggaa tggctgcccg atcgacttct tgtaggagta cgcggcgatc gtcgggagct
     2761 tggcgatgag acggacgatc gccagttgca cctgatccgg atcgtgcgga tctgcgctgt
     2821 cagggtagaa ggtcgacagg gcgctgacga ccgatgagag cgcccccatc gggtgggcgt
     2881 catgcgggaa cccgtcgaag aaccgctttg cctcctcgtg gagcagcgtg tgcttgcgga
     2941 tcgatcgact gaactcgtct cgctgggcgc gtgtagggag ctcgccgtag atcaggaggt
     3001 acgcggtctc gagaaacgac ggcgagtggc cggacacgag ctgttcgatt gggatccccc
     3061 ggtagcgcaa gatgccctga tcaccgtcga tgaacgtgat ggccgactcg cacgatccgg
     3121 tgttcaccaa cccgtagtcg agcgtgacca gaccggactg cgcgcgcagc ttcgcgatgt
     3181 cgacaccgag ctcgttctcg gtgccgcgca cgacgggcag ctcgatctcg gtgtcgccg
//
LOCUS       DATCCH010000201         2062 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_52209, whole genome shotgun sequence.
ACCESSION   DATCCH010000201 DATCCH010000000
VERSION     DATCCH010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52209"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VIJ44_03995"
     CDS             <1..384
                     /locus_tag="VIJ44_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165095.1"
                     /translation="AEPIANLLTRGRAGPSGWLRTYRGHGLGTDPLDAPGDQDVTCDL
                     AIESVRRAAHHAGLRVRAELAQAEWLRSLGLDALVDEGRRAWADGAHRGDLRAIAARS
                     RVVEGAALTDPSGLGGHRVVLLSRP"
     gene            508..>2062
                     /gene="acs"
                     /locus_tag="VIJ44_04000"
     CDS             508..>2062
                     /gene="acs"
                     /locus_tag="VIJ44_04000"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEY5165096.1"
                     /translation="MSDTIESLLAEGRTFPPPPDFKRHALVTDAEIYDDANVDREGFW
                     ALQASQLLDWYEEWHTILEWDLPFAKWFVGGKLNATYNCLDRHVDAGHGDQVAYHWEG
                     EPGDTRTITYSQLLDDVSQLANALLALGVRKGDRVNIYLGMVPELPMSLLACARIGAA
                     HSVVFGGFSSDSLRDRINDAEAKVLITADGAWRRGSTVALKATADLALDECPTIERVL
                     VLQRTGQADVAMTDGRDVWWHDLVPNQATECPPEPMDSEDLLYLLYTSGTTAKPKGIM
                     HTTGGYLTQVAFTHKYVFDLKPDTDVYWCAADVGWVTGHSYIVYGPLMNRTTSVMYEG
                     TPDFPDKDRLWQIVEKYGVTILYTAPTAIRTFMKWGEEYPDRHGLSSLRLLGTVGEPI
                     NPEAWVWYWQHIGGGRCPVVDTWWQTETGAIMISPLPGATTLKPGSATFPLPGIGAEI
                     VDRDGANVPVPGGGYLVLTHPWPAMLRGIWGDPQRYRDTYWERFDGRYFAGDGAKRDE
                     EGYYWLLGRG"
BASE COUNT          351 a          771 c          648 g          292 t
ORIGIN      
        1 gccgagccga tcgcgaacct cttgacccga ggacgagccg gaccgtcggg gtggctgcgg
       61 acctaccggg gccacgggct cggcacggac ccgctcgacg cgcccggtga ccaagacgtg
      121 acgtgtgacc tggcgatcga atcggtgcgg cgagccgcgc accatgccgg gctgcgcgtc
      181 cgtgccgagc tcgcccaagc agaatggctc cggagcctcg gcctcgacgc gctggtcgac
      241 gaaggacgcc gggcctgggc cgatggtgca caccgtggcg acttgcgcgc gatcgcagcg
      301 cgcagccgcg tcgtcgaagg cgcggcgctc accgacccgt cgggcctcgg cggccaccgg
      361 gtcgtgctgc tcagccgccc atgaaccaat ccagcgcgga cccgaacacc cgtcatccgg
      421 cgcggccggg ctcgagcgac cgccgctccg cgaggctgcg ttagcctcac ccgcgctgtc
      481 gagcgccgaa cgaacggggg aacgcgcatg tccgacacga tcgagtccct gctcgccgag
      541 gggcgcacgt tccctcctcc gccggacttc aagcggcacg ccctcgtcac ggacgccgag
      601 atctacgacg acgcgaacgt cgaccgggag ggcttctggg ctctccaggc cagccagctc
      661 ctcgactggt acgaggagtg gcacaccatc ctcgagtggg acctcccctt cgccaaatgg
      721 ttcgtcggcg gcaagctcaa cgccacctac aactgcctcg accgccacgt cgacgcgggc
      781 cacggtgacc aagtcgccta ccactgggaa ggcgaacccg gcgacacccg cacgatcacc
      841 tactcgcagc tcctcgacga cgtgtcacaa ctcgccaacg cgctcctcgc actcggggtc
      901 cgcaagggcg accgggtcaa catctacctc ggcatggtgc ccgagctccc catgtcgctc
      961 ctggcctgcg cccggatcgg tgctgcgcac tctgtcgtct tcggaggctt ctcctccgac
     1021 tccctgcgcg accgcatcaa cgacgccgag gccaaggtgc tgatcaccgc cgacggcgcc
     1081 tggcgtcggg ggagcaccgt cgccctcaag gccaccgccg acctcgcgct cgacgaatgc
     1141 cccaccatcg agcgcgtgct cgtcctccag cggaccggac aggccgacgt cgccatgacc
     1201 gacggccgcg acgtctggtg gcacgacctc gtgcccaacc aggccacgga atgcccacct
     1261 gagccgatgg acagcgaaga cctcctctat ctcctctaca ccagcggcac cacggcgaaa
     1321 cccaaaggga tcatgcacac cacggggggg tacctcaccc aggtcgcctt cacccacaag
     1381 tacgtcttcg acctcaagcc cgacaccgac gtgtactggt gcgccgccga cgtcggctgg
     1441 gtgaccggcc actcgtacat cgtgtacggg ccgctcatga accgaaccac cagcgtcatg
     1501 tacgaaggca ccccggattt ccccgacaag gaccgcctgt ggcagatcgt cgagaagtac
     1561 ggcgtcacga tcctctacac cgcgcccacc gcgattcgca ccttcatgaa atggggtgag
     1621 gagtatcccg accgtcacgg cctctcctcg ctgcggctcc tcgggacggt cggcgagccc
     1681 attaaccccg aggcgtgggt ctggtactgg caacacattg gtggtggacg ctgcccggtt
     1741 gtcgacacct ggtggcagac cgagaccggc gccatcatga tcagccccct tccgggggcc
     1801 accaccctca aaccgggaag cgcgacgttc ccgctcccgg gcatcggtgc cgaaatcgtc
     1861 gaccgcgacg gcgccaacgt cccggtaccg ggcggtggct acctggtgct cacccatccg
     1921 tggcccgcga tgctccgggg catctggggt gacccgcagc ggtatcgcga cacgtattgg
     1981 gaacggttcg acgggcgata cttcgccggg gacggcgcca aacgcgacga ggagggctac
     2041 tactggctcc tcggccgggg cg
//
LOCUS       DATCCH010000202         3392 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_343146, whole genome shotgun sequence.
ACCESSION   DATCCH010000202 DATCCH010000000
VERSION     DATCCH010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343146"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..1880
                     /locus_tag="VIJ44_04005"
     CDS             111..1880
                     /locus_tag="VIJ44_04005"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5165097.1"
                     /translation="MEQDHQSRRDGWRLMRSALRSQRRWLSLGILAGGLWTGARLAGP
                     LLAAVAIDQGILPGNGHIVVVYALLILFVGVIQAIGTALRRYTAFRIAYRVETDLRER
                     LFAHLQQLHFAFHDRAQTGQLMARANSDIQQVNLVVVLIPLTVATTFLLSGVIIVMVL
                     HNVLLAVFALGALPLLSIAAARFSHRLHPVSLQLQNELGDFSGVVEESVAGVRVVKGF
                     GTEARQEAQLAVEADQVLDRALAGARLRANFLPLVDFLPALALVIILGYGGHLVLGGQ
                     LQIGDLVAFNSYILYLLWPLRLVGMLVAQAARSSAAAGRIDELLATDTAVADPTNPSA
                     LPARGGGDLRFDDVSFAYGQGPPVLDGFELVVHPGEAVAVVGATASGKTTIARLLPRF
                     YDVDAGRVLLDGVDIRQLRLRELRRAIGIVFEDTFLFTDTVAENIAFAEPDASMDLVR
                     NAARLAGADEFIERLPNGYDTLIGEHGYSLSGGQRQRVAIARAVLADPRVLILDDATS
                     SVDPTKEHEIRSALREVMRDRTTLIIAHRAATIALADRVVLLDHGRVVAEGTHEELLN
                     STERYRHVLAQSGAVLPVAGATD"
     gene            1880..>3392
                     /locus_tag="VIJ44_04010"
     CDS             1880..>3392
                     /locus_tag="VIJ44_04010"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5165098.1"
                     /translation="MGTDARRQAFATDDELLTREDAQTLLRRLWTLLDPYHAKIAGAV
                     TLIALWTGCLLAGPALVKHGIDAGLRGKDEGALDLSAGLYLAVALGGLFFARSAIWAV
                     SKVGEPFLRDLRNRVFRHLMSLDLGFFEREQTGRLVARLTSDVDAMQDLVQIGLAQFA
                     QNIFLFVGAVVVILVLSWQLALCTLVVVPPVVWASRWFRRESSRAFLEVRDRIGHNLA
                     TLQEGLAGVRVVQAFGRERAFARRFRETNEAQYDANLETVRISTRYFPFIEFCGVAGT
                     ALIVGVGGLFADQRIVTVGTVAAFVLYLNSLFEPVQQLSQLYNTLQQAGAAMKKLVGL
                     LDTQPRIAERAGAVDLPPTGVLEVEDVSFRYGDGPLVLRHVSVSVGDGERLALVGPTG
                     AGKSTLAKLMSRFYDPAEGNVRFGGVPLTHATLASLRDRIIVVPQEGFLFAGTIRDNV
                     RVGRASASDDDVEAALDALGILDRFRALPSGLDTEVRERGSRLSAGERQLVSLA"
BASE COUNT          458 a         1336 c         1053 g          545 t
ORIGIN      
        1 cgcgcctcgc gaatcgccgc cagccgcgac gatcgcccct ggtaacctgg gccgctggcc
       61 tgagcgacgt tgcacgccgg gcacgttcgc ggtcgcagga gctgaaggcg ttggagcagg
      121 accaccagtc gcgtcgggac ggctggcggc tcatgcggtc ggcgctccgc tcccagcgcc
      181 gttggctcag cctcgggatc ctcgccggtg ggctgtggac cggggcccgg ctcgcgggac
      241 cgctcctcgc cgcggtcgcc atcgaccagg ggatcctgcc gggcaacggc cacatcgtcg
      301 tcgtgtacgc gctgctcatc ctcttcgtcg gcgtcatcca ggcgatcggc accgcgctgc
      361 ggcgctacac cgcgttccgg atcgcgtacc gggtcgagac cgacctgcgc gagcggctct
      421 tcgcccacct ccaacagctc cacttcgcgt tccacgaccg ggcccagacc ggccagctca
      481 tggcccgggc gaacagcgat atccaacaag tcaacctcgt cgtcgtcctc atccccctga
      541 cggtggccac cacgttcctg ctctcgggcg tgatcatcgt gatggtcctc cacaacgtgt
      601 tgctggcggt gttcgcgctc ggtgccctgc cgctgctcag catcgccgcg gcgcgcttct
      661 cgcaccggct gcaccccgta tcactccaac tccaaaacga gctcggcgac ttctccggcg
      721 tcgtcgagga gagcgtcgcc ggggtccggg tcgtgaaggg cttcgggacc gaagcccgcc
      781 aggaagccca gctcgcggtc gaggccgacc aggtgctcga ccgggcgctc gccggcgcgc
      841 gcctgcgcgc caacttcttg cccctcgtcg acttcctccc cgccctcgcc ctggtgatca
      901 tcctcgggta cggcggccac ctggtgctcg ggggccagct gcagatcggt gacctcgtcg
      961 cgttcaactc ctacatcctc tatctgctct ggccactccg cctggtcggg atgctcgtcg
     1021 cccaggcggc gcgctcctcg gccgccgcgg gacgcatcga cgagctgctc gccaccgaca
     1081 cggccgtcgc cgaccccacc aacccgagcg cgctgcccgc ccgcggcggc ggcgacctgc
     1141 gattcgacga cgtgtccttc gcctacggcc aaggcccacc ggtgctcgac ggcttcgagc
     1201 tcgtcgtgca ccccggtgag gcggtcgcgg tggtgggggc gaccgccagc gggaagacca
     1261 ccatcgcccg gctgctcccc cgcttctacg acgtcgacgc cggccgtgtc ctcctcgacg
     1321 gtgtcgacat ccgccagctg cgcctccggg agctgcgacg cgccatcggg atcgtgttcg
     1381 aagacacctt cttgttcacc gacaccgtcg ccgagaacat cgccttcgct gaacccgacg
     1441 cgtcgatgga cctcgttcgc aacgcggccc gcctcgccgg agccgacgag ttcatcgagc
     1501 ggctcccgaa cggctacgac accttgatcg gcgaacacgg ctactcgctg tccggcgggc
     1561 agcggcaacg agtcgcgatc gcccgcgccg tcctcgccga cccccgggtg ctgatcctcg
     1621 acgacgccac atcctcggtc gacccgacca aggaacacga gatccgctcc gcactgcgcg
     1681 aggtcatgcg cgaccggacg acgctgatca tcgctcaccg cgccgcgacg atcgccctcg
     1741 ccgaccgggt ggtcctcctc gaccacggtc gcgtcgtcgc cgagggcacc cacgaggagc
     1801 tcctcaactc caccgagcgg taccgccacg tgctcgccca gtccggcgcg gtcctcccgg
     1861 ttgccggagc gaccgactga tgggcaccga cgcgcgtcgc caggccttcg ccaccgacga
     1921 cgagttgctt acccgcgagg acgcccaaac gctcctgcgc cgcctctgga cgctcctcga
     1981 cccgtatcac gccaaaatcg ccggcgcggt gacgctcatc gcgctctgga ccggctgcct
     2041 cctcgcgggg cccgccctgg tgaagcacgg catcgacgct gggctgcgcg gcaaggacga
     2101 gggcgccctc gacctctccg ccgggctcta cctcgccgtc gccctcgggg gtctgttctt
     2161 cgcccggtcc gccatctggg cggtctcgaa ggtgggagag ccgttcctcc gcgacctccg
     2221 caaccgggtg ttccgccacc tcatgtccct cgacctcggc ttcttcgagc gcgagcagac
     2281 cgggcgtctc gtcgcccgcc tcacctccga cgtcgacgcg atgcaggacc tggtccagat
     2341 cggcctcgcc cagttcgccc agaacatctt tctcttcgtg ggcgcggtcg tcgtcatcct
     2401 ggtgctgtcc tggcagctgg cgctgtgcac cctcgtcgtc gtcccaccgg tggtgtgggc
     2461 cagccgctgg ttccggcgcg agtcaagccg cgccttcctc gaggtccgcg accgcatcgg
     2521 acacaacctc gccacgctcc aagagggcct ggccggcgta cgcgtggtgc aggccttcgg
     2581 ccgcgagcgg gccttcgccc gccgcttccg ggagaccaac gaagcgcagt acgacgccaa
     2641 cctcgagacg gtcaggatca gcacccgcta cttcccgttc atcgagttct gtggcgtcgc
     2701 gggcaccgca ctcatcgtcg gcgtcggcgg cctcttcgcc gatcagcgca tcgtcaccgt
     2761 cggcaccgtc gccgcgttcg tcctctatct caacagcctt ttcgaaccag tccaacagct
     2821 cagccagctg tacaacaccc tgcagcaagc cggcgcggcc atgaagaagc tcgtcgggct
     2881 gctcgacacc cagccccgaa tcgccgagcg agccggcgcg gtcgaccttc cccccaccgg
     2941 cgtcctcgag gtcgaggacg tgtcgttccg ctacggcgac gggccgctcg tgctccgtca
     3001 cgtcagcgtg tcggtcggcg acggggaacg gctcgcgctc gtcgggccga ccggcgccgg
     3061 caagtccacc ctcgccaagc tcatgtcccg cttctatgac cccgccgagg gcaacgtccg
     3121 cttcggcggc gttccgctca cccacgccac cttggcgtcg ctgcgcgacc gcatcatcgt
     3181 cgtcccccaa gagggattcc tcttcgccgg gacgatccgc gacaacgtcc gggtcggccg
     3241 agccagcgcg tccgacgacg acgtcgaagc cgccctcgac gctctcggga tcctcgaccg
     3301 gttccgggcc ctcccctcgg gtctcgacac cgaggtccgc gaacgcgggt cgcgcctgtc
     3361 ggccggggaa cgacagctcg tctcactcgc cc
//
LOCUS       DATCCH010000203         6249 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_520286, whole genome shotgun sequence.
ACCESSION   DATCCH010000203 DATCCH010000000
VERSION     DATCCH010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6249
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520286"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="VIJ44_04015"
     CDS             <1..303
                     /locus_tag="VIJ44_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00374 family protein"
                     /protein_id="HEY5165099.1"
                     /translation="LAAVFYGFCLQACLAAFGATLSFWSLLAADIFLGTLASLVPIPG
                     GSTAVASVGLSGALVAFGLPKEIAVAAVLTNQLVVNYLPAVPGWVATTRLVRRGFI"
     gene            402..1793
                     /gene="tig"
                     /locus_tag="VIJ44_04020"
     CDS             402..1793
                     /gene="tig"
                     /locus_tag="VIJ44_04020"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEY5165100.1"
                     /translation="MSPLTTSVEPLEGNKVRLRVAVPADEFEQAVDAAFRKLARQVKI
                     PGFRPGKAPRQLLEARLGGEAAREQALRDSVPDYYAQAIEAEDIDVIAAPEIDITEGE
                     DGGDVAFDAVVEVRPIVELAGYDPLRVEIPATEITDDAVEAQVDALRERFADLEDKTG
                     PLADGDYAQIDIKAYVHDEVVDALSATDFLYEVGSGGLVPKLDEELRSKRVGDILKFN
                     DQLPERFGDRAGDEVSFQVLIKETKRKILPEPTDEWASEVSEYDTVDALRDDARNRLS
                     LVAKVRAQMLVRDKVLEAVAKLVDTEIPDALVTSETERRLHDLIHRLEHEGATLPDYL
                     AATGQDQNAFVNQVRDGAIDAVRADLALRAVVNQEHIEATDEDVDQEVDRLAERLKAK
                     PAKVRRDLERGGMFEAVRSDIARGKALQLLVDHADVVDESGNPVDLTLADDGTPNPET
                     DPPSEDPQEQEPV"
     gene            1790..2446
                     /locus_tag="VIJ44_04025"
     CDS             1790..2446
                     /locus_tag="VIJ44_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEY5165101.1"
                     /translation="MSYRPPDQPLPAYSYLVPTVVEQTSRGERAFDLYSRLLKERIIF
                     LGTPIDDMVANLLMAQLIHLESEDPDKDISLYINSPGGEITALFAIYDTMQYIKPDVQ
                     TICIGQAASAAAVLLASGAPKKRYALPHARILIHQPHGGASGQAVDIEIQAKEITRMR
                     ELLDQILAYHTGQTVEKVGTDTDRDFIMSADEAQTYGIVDEILTNRELASVGTAAGVS
                     "
     gene            2475..3728
                     /gene="clpX"
                     /locus_tag="VIJ44_04030"
     CDS             2475..3728
                     /gene="clpX"
                     /locus_tag="VIJ44_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006542686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEY5165102.1"
                     /translation="MAKFGDGGDLLKCSFCGKSQKQVKKLIAGPGVYICDECIDLCNE
                     IIEEELSQSSEVRFDELPKPREIYEYLNDYVIGQDQAKKILSVAVYNHYKRVQAGAYG
                     DPEVELQKSNILLIGPTGCGKTLLAQTLARLLKVPFAIADATALTEAGYVGEDVENIL
                     LKLIQAADYDVKKAETGIIYIDEIDKIARKAENPSITRDVSGEGVQQALLKILEGTTA
                     SVPPQGGRKHPHQEFIQIDTTNILFLCGGAFAGIDKIIESRTGNRGVGFGADVRRPEE
                     KDMGALLAGVLPEDLLKFGLIPEFVGRLPVVGAVHNLDEDALMRILIEPKNALLKQYQ
                     KFFQFDDVDLVLTDDALEAVADEALKRGTGARGLRAILEEVLLDVMYDLPSRSDVARC
                     VIDRSVVLEHMTPTLITNAADRRSA"
     gene            3839..>6249
                     /gene="valS"
                     /locus_tag="VIJ44_04035"
     CDS             3839..>6249
                     /gene="valS"
                     /locus_tag="VIJ44_04035"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017581024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEY5165103.1"
                     /translation="MLVPGKPSLDGLEEVWSKRWDADGTYRFDRSKTRDQIFSIDTPP
                     PTVSGSLHVGHVFSYTHTDTVARFQRMRGREVFYPIGWDDNGLPTERRVQNFFGVRCD
                     PSLLYDPDFEAPPKPSNPPAAISRRNFVELCERLVVEDEEKFEELWRRLGLSVDWTLT
                     YTTIGKASQRISQRGFLHLLARGEAYQAEAPTLWDVDFRTAVAQAELEDRETPGAYHA
                     VRFHRPSGDPVVIETTRPELIPACVALVAHPDDTRYQPLFGSVVRTPLFDVEVPVVAH
                     PLADPEKGSGIAMICTFGDLTDVTWWRELDLPARPVIDWDGRLKADPPDVITADAGRA
                     AYADLAGKTIRQAQRRVVELLGEAGELVGDPKPVTHAVKYYERGDRPLEIITTRQWYI
                     RNGGRDPNLRGELLARGRALQWHPAYMRARYESWVEGLNGDWLISRQRFFGVPFPLWY
                     PVDADGQADHDHPMLASEDQLPVDPASEVPDGYDETQRGQPGGFVGDPDIMDTWATSS
                     LTPQIACRWNDDPDLFTRTYPMDLRPQAHDIIRTWLFSSVVRAHLEFGALPWQHAAIS
                     GWVLDPDRKKMSKSKGNVVTPMATLEQYGSDAVRYWAASGRPGTDTSFEEGQMKIGRR
                     LAIKLLNASKFTLGVAGGDGEVPEPAAVRAPLDRALLATLADLVTETTVAFEGYDYAR
                     ALDHTERFFWRFCDDYVELVKQRAYGSRGEEAAASASAALVLALDTMLRLFAPHLPFV
                     TEEVWSWWRPGSIHHADWPSPDPLRAVTGEGESAGDPAVFSAAADLLGQVRKAKSEQQ
                     RSLRV"
BASE COUNT         1102 a         2232 c         1960 g          955 t
ORIGIN      
        1 ctcgcggccg tgttctacgg attctgcctc caggcctgcc tcgccgcgtt cggcgccacc
       61 ctttccttct ggtccttgct cgcggccgac atcttcttgg gcaccttggc gtcgctggtc
      121 ccgatccccg gcgggagcac cgcggtggcg tcggtcggcc tctcgggggc gctggtggcg
      181 ttcggtctcc cgaaggagat cgcggtggcg gcggtcctca ccaaccagct ggtcgtcaac
      241 tacctgccgg cggtgccggg ttgggtcgcg acgacccgcc tggtgcgccg cggcttcatc
      301 tagggcgcac cggccgggcg cggccggcct ccgggcggcc cgactggcgg gattcggtgc
      361 ataggacctc cggcacccac gggtatcctg cgcttctcct tatgtcgcct ctcactacgt
      421 cggtcgagcc actcgagggc aacaaggtcc ggctccgagt cgccgtgccc gccgacgagt
      481 tcgaacaggc cgtcgacgcc gcattccgca agctggcccg ccaggtgaag atcccggggt
      541 tccggcccgg caaggcccct cgccaactgc tcgaagctcg cctcggcggc gaagcagccc
      601 gggagcaggc cctccgcgac agtgtgcccg actactacgc ccaggcgatc gaggccgaag
      661 acatcgacgt catcgccgcc cccgagatcg acatcaccga gggtgaagac ggcggagatg
      721 tcgccttcga cgccgtcgtc gaagtgcgcc ccatcgtcga gcttgcgggg tacgacccgc
      781 tccgagtcga gatcccggcc accgagatca cagacgacgc ggtcgaggct caagtcgacg
      841 ccctccggga gcgcttcgcc gacctcgagg acaagaccgg cccgctcgcc gacggcgact
      901 acgcccagat cgacatcaag gcgtacgtcc acgacgaggt ggtcgacgcc ctcagcgcaa
      961 ccgacttcct gtacgaggtc ggctccggag gactggtgcc gaagctcgac gaggagctgc
     1021 gcagcaagcg ggtcggcgac atcctcaagt tcaacgacca gctccccgaa cgattcgggg
     1081 accgcgccgg cgacgaggtc tcgttccagg tcctgatcaa agagaccaaa cgcaagatcc
     1141 tccccgaacc caccgatgaa tgggcgtccg aggtcagcga gtacgacacc gtcgacgccc
     1201 tccgcgacga cgcccggaac cgtctctcgt tggtcgcgaa agttcgagca cagatgctgg
     1261 tgcgcgacaa ggtgctcgaa gcggtcgcca agctcgttga caccgagatc cccgatgctc
     1321 tggtcacgtc agagaccgag cggcgcctgc acgacctgat ccatcgtctc gagcacgaag
     1381 gcgcgacgct cccggactat ctcgccgcca ccggtcaaga ccagaacgcc ttcgtcaacc
     1441 aggtacgcga cggtgccatc gatgccgtgc gcgccgatct cgccttgcgt gcggtcgtca
     1501 accaggagca catcgaagcg accgacgaag acgtcgatca ggaagtcgac cgcctcgccg
     1561 agcgcctcaa ggccaagccg gccaaggtgc gacgcgatct cgaacggggt ggcatgttcg
     1621 aggcggtacg atcggacatc gctcgcggca aggcactcca gttgctcgtc gatcacgccg
     1681 acgtcgtcga cgagtccggc aatcccgtcg atctcaccct cgctgacgac ggcaccccca
     1741 accccgaaac cgatccgccg agcgaagacc ctcaggaaca ggagccagtg tgagttaccg
     1801 cccccccgac cagcctctcc ccgcctacag ctatctggtg ccgacagttg tcgagcagac
     1861 cagccgtggt gagcgcgcct tcgatctgta ctcgcggctc ctcaaggaac ggatcatctt
     1921 cctcgggacc ccgatcgacg acatggtcgc caacctgctc atggcgcagt tgatccatct
     1981 cgagagcgaa gacccggaca aggacatctc gctgtacatc aactcgcccg gtggcgagat
     2041 cactgcgctg ttcgcgatct acgacacgat gcagtacatc aagccggacg tgcagacgat
     2101 ctgcatcggg caggccgcct ccgccgcggc ggtgctcctc gcatccgggg caccgaagaa
     2161 gcgctacgcg ctgccccacg cgcggatcct catccaccag ccccacggcg gagcctccgg
     2221 acaggccgtc gacatcgaga tccaggccaa agagatcacc cggatgcgcg agctgctgga
     2281 ccagatcctg gcgtaccaca ccggccagac ggtcgagaag gtcggcaccg acaccgaccg
     2341 ggacttcatc atgagcgccg atgaggctca gacctacggc atcgtcgacg agatcctcac
     2401 caatcgcgag ctcgcgtcgg tcgggaccgc ggccggcgtg agctgagctc aggccatcga
     2461 gggggaggga cagcgtggcc aagttcggtg acggaggtga cctgctgaaa tgctcgttct
     2521 gcgggaagag ccagaagcag gtcaagaagc tcatcgccgg tcccggtgtc tacatctgtg
     2581 acgagtgcat cgacctctgc aacgagatca tcgaggaaga gctctcccag agctccgaag
     2641 tccgcttcga cgagctcccc aagcctcgtg agatctacga gtacctgaac gactacgtca
     2701 tcggacagga ccaagccaag aagatcctca gcgtcgccgt ctacaaccac tacaagcggg
     2761 tccaagccgg cgcgtacggc gaccccgagg tcgagctgca gaagtcgaac atcttgctca
     2821 tcggcccgac tggctgcggc aagacgctcc tcgcccagac cctcgctcgg ctgctcaagg
     2881 tgccgttcgc gatcgccgac gccaccgcgt tgaccgaggc cggttacgtc ggcgaagacg
     2941 tcgagaacat cttgttgaaa ctgatccaag ccgccgacta cgacgtgaag aaggccgaga
     3001 ccgggatcat ctacatcgac gagatcgaca agatcgcccg caaggccgag aacccgtcga
     3061 tcacccgcga tgtctccggt gaaggtgtgc agcaggcgct gctgaagatc ctcgagggca
     3121 ccaccgcctc ggtgccaccc caaggtggcc gcaagcatcc ccaccaagag ttcatccaga
     3181 tcgacacgac gaacatcttg ttcctctgcg gtggcgcctt cgccgggatc gacaagatca
     3241 tcgagagccg caccgggaac cgcggggtcg ggttcggtgc cgacgtccgc cggcccgagg
     3301 agaaagacat gggtgcgctc ctcgccgggg tgctccctga agacctcctc aagttcgggc
     3361 tcatccccga gttcgtcggg cgtctcccgg tcgtcggcgc cgtgcacaac ctcgacgaag
     3421 acgccctgat gcggatcctg atcgagccca agaacgcgct gctcaagcag taccagaagt
     3481 tcttccagtt cgacgacgtc gatctcgtgc tcaccgacga tgcgctcgag gccgttgcgg
     3541 atgaggccct gaagcgaggg accggcgcgc gcggcttgcg ggccatcctc gaagaagtgc
     3601 tcctcgacgt catgtacgac ctcccgagcc gctcggacgt ggcccgctgt gtcatcgacc
     3661 gcagtgtcgt actcgagcac atgaccccga cgctgatcac gaacgccgcc gaccgccgct
     3721 ccgcctgacg ccgctccggg gccacgcggc ctcgcggcac cgccggaccg tcaccgcggg
     3781 cggccgacct cgagccgatg tgggtcgtga cgcgcgggcg tctctctaga ctcacgccat
     3841 gctggtccct ggcaaaccca gcctcgacgg gctcgaggag gtctggagca aacgctggga
     3901 cgcggacggc acgtaccgct tcgaccgctc caagacccgg gaccagatct tctccatcga
     3961 cacgccgcca cccaccgtgt cggggtcgtt gcacgtcggc cacgtcttct cgtacaccca
     4021 caccgacacc gtcgcccggt tccagcggat gcggggccga gaggtgttct acccgatcgg
     4081 ctgggacgac aacggcctgc caaccgagcg gcgggtgcag aacttcttcg gtgtccgctg
     4141 cgacccctcc ctcctgtacg acccggactt cgaggccccc ccgaagccgt ccaatccgcc
     4201 cgcggcgatc tcgcgtcgga acttcgtcga gctgtgcgag cgtctcgtcg tcgaagacga
     4261 ggagaagttc gaagaactct ggcgccgcct cgggctctcc gtggactgga ccctcaccta
     4321 cacgacgatc gggaaggcca gccaacggat ctcccaacgc ggctttctgc acttgctggc
     4381 ccggggcgag gcgtaccaag ccgaggcacc cacgttgtgg gatgtcgact tccgtaccgc
     4441 ggtggcccaa gccgaactcg aagaccgcga gacgcccggc gcttaccacg cggtgcggtt
     4501 tcatcggccg tcaggtgacc cggtcgtcat cgagacgacc cgccccgagc tcatccccgc
     4561 gtgcgtcgcc ctcgtcgcgc atcccgacga cacccgctac cagccgctgt tcgggtcggt
     4621 ggtgcgcacc ccgctcttcg atgtcgaggt ccccgtcgtc gcgcacccac tcgccgatcc
     4681 cgaaaagggt tccggcattg cgatgatctg caccttcggt gacctcaccg acgtcacctg
     4741 gtggcgcgag ctggacctgc cggcacgacc ggtcatcgac tgggacgggc gcctcaaggc
     4801 cgacccacct gacgtcatca ccgccgacgc cggccgcgcc gcatacgccg acctcgccgg
     4861 gaagacgatc cgccaagccc agcgacgcgt ggtcgagctc ctcggcgaag ccggcgagct
     4921 ggtcggcgat ccgaagccgg tcacccacgc ggtcaagtac tacgagcgcg gagaccggcc
     4981 cctcgagatc atcaccaccc ggcagtggta catccgcaac ggcggccgcg acccgaacct
     5041 gcgaggcgag ctcctcgccc ggggtcgggc gctgcagtgg catccggcgt acatgcgggc
     5101 ccgctacgag tcgtgggtcg agggactcaa cggcgactgg ctcatcagcc gccagcggtt
     5161 cttcggcgtg ccgttccccc tgtggtaccc ggtcgatgcc gacggccagg cggatcatga
     5221 ccacccgatg ctggcgtccg aagaccagct tccggtcgac cccgcgtcgg aggtgcccga
     5281 cggctacgac gagacccagc gtggccagcc cggcgggttc gtcggcgacc ccgacatcat
     5341 ggacacctgg gccacgtcgt cgctgacacc gcagatcgcg tgtcggtgga acgacgaccc
     5401 cgacctcttt acccgtacct acccgatgga tctccgtccc caggcccacg acatcatccg
     5461 cacgtggctg ttctccagtg tcgtgcgcgc ccacctcgag ttcggggcgc tgccgtggca
     5521 gcacgcggcc atctcgggct gggtcctcga ccccgaccgc aagaagatgt cgaagtcgaa
     5581 aggcaacgtc gtcaccccga tggccaccct cgagcagtac gggtccgacg cggtccgcta
     5641 ctgggcggca agtggccggc cgggtacgga cacctcgttc gaggaaggcc agatgaagat
     5701 cggccggcgc ctcgcgatca agctcctcaa cgcgtccaag ttcaccctcg gcgtcgccgg
     5761 tggcgacggc gaggtccctg aaccggccgc ggtgcgcgcg ccactcgacc gggccctgct
     5821 cgccaccctc gcggaccttg tgaccgagac gaccgtggcg ttcgagggct acgactacgc
     5881 ccgcgcgctg gatcacacgg aacggttctt ctggcggttc tgcgacgact atgtcgagtt
     5941 ggtcaagcaa cgtgcgtacg gaagccgggg agaggaggcg gccgcgtcgg cgagcgccgc
     6001 cctcgtcctt gctctcgaca cgatgctgcg cctgttcgca ccccacctcc cgttcgtcac
     6061 cgaagaggtc tggtcgtggt ggcgacccgg ttcgatccat cacgccgact ggcccagccc
     6121 ggatccgctg cgggcggtca ccggcgaggg tgagtcggcc ggtgatccgg cggtgttcag
     6181 cgcggccgcc gacctgctcg gccaggtgcg caaggccaag agcgagcagc aacgatcctt
     6241 gcgggtcga
//
LOCUS       DATCCH010000204         2393 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_188411, whole genome shotgun sequence.
ACCESSION   DATCCH010000204 DATCCH010000000
VERSION     DATCCH010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188411"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..291)
                     /locus_tag="VIJ44_04040"
                     /pseudo
     CDS             complement(22..291)
                     /locus_tag="VIJ44_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_273701366.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UGSC family (seleno)protein"
     gene            complement(300..1742)
                     /locus_tag="VIJ44_04045"
     CDS             complement(300..1742)
                     /locus_tag="VIJ44_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014376572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEY5165104.1"
                     /translation="MTVSLPDGLVAIVKRDCPTCQLVTPVLAQLRDASGAPPLTIYTQ
                     DDAAFPPDLDPVDDTGLAVSLALDLDTVPTLIRVEDGRETARLVGWSRDQWEAFTGLD
                     GLGAGLPPHRPGCGSRTQDPDLAAALADTTAARRLGSRRVELGASEDDLEAMFERGWS
                     DGLPVVPPTPERVARMLTGTTRAPDDVVAVVPPDLVECSVEKVAINAVLAGCRPDYLP
                     VVLASVEAACTDEFNMHGLLATTWFSGPVVIVNGPVARTIGMNAGINAMGQGNRANAT
                     IGRALQLVVRNVGGGRPGEVDRSTLGQPGKYTFCFAEREVDSPWEPLHVTRGFPAESS
                     AVTLFAGEAPRGIVDQLSRDPASLANTFAACLRATTHPKIALAFDAVLVVSPEHGRVF
                     REAGWSKTQLLEELTSRLQLPGAELVRGAGGIAEGLPASVETLTIPKFRDGGLLIVHT
                     GGGAGLFSAIIGGWASGPGGSAPVTKEIGT"
     gene            complement(1739..>2393)
                     /locus_tag="VIJ44_04050"
     CDS             complement(1739..>2393)
                     /locus_tag="VIJ44_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010308774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY5165105.1"
                     /translation="VMMDIARPEADGLVLVDGTSAAGGLRVDPDAFDVYYFAPQKAFG
                     SDGGLWFALCSPAAVERIERIGASGRWQPAFLSLPIALENSRQDQTYNTPALTTLFLM
                     VDTVEWMVAHGGLEWAATRCDRAAEVLYGWAEASPYATPFVAKPAERSHVTATIDFDG
                     VDATQLAAALRTNGIVDTESYRKLGRNQLRIALFPAIELDDVVTLTLAIDHLVAALQ"
BASE COUNT          340 a          815 c          898 g          340 t
ORIGIN      
        1 gcacgacgtg cacgatcctc aatcggcgag cgcctcgatg acgacgtcgc actgctcgga
       61 gatctcgtgg cggagatcga tcggcgcgac cttggtgaag gtcggcttgg cgtagcgaac
      121 gactcgcccg ccgcgtgcgg cgacatgctg ctcgatccgg tcgaggaaga tgtcaccgcg
      181 ggccttcctg atgtcgagga gcccgaccgt gagcccgttc agcgtcgccg gccggggcgt
      241 ccgctcccgg gtgggggcgt gtcgttcgcc ggtcggatcg aggaccacca ggtttccgct
      301 cacgtgccga tctccttcgt caccggggcg ctcccgccag ggccgctggc ccagcccccg
      361 atgatggccg agaacaggcc cgcgccacca ccggtgtgca cgatgaggag gccgccgtct
      421 cggaacttcg ggatggtgag ggtctccacc gaggccggga gcccttcggc gatgccgccc
      481 gcgccccgga ccagctccgc gccggggagc tggagccggc tcgtgagctc ttcgaggagc
      541 tgggtcttgc tccacccggc ctctcggaac acccggccgt gctcgggtga caccacgagc
      601 acggcgtcga acgccagggc gatcttggga tgcgtcgtcg cgcgcaggca cgccgcgaac
      661 gtgttggcca gcgacgccgg gtcgcgggac agctgatcga cgatgccccg gggggcctca
      721 ccggcgaaga gggtgaccgc ggacgactcg gcgggaaagc cccgggtcac gtgcagcggc
      781 tcccacggtg aatcgacttc ccgctcggcg aagcagaacg tgtacttgcc gggctggccc
      841 agtgtcgacc ggtcgacctc gccgggccgg ccgccaccaa cgttgcggac gacaagctgg
      901 agggcccgcc cgatcgtggc gttcgcccgg ttgccttggc ccatggcgtt gatgcccgcg
      961 ttcatcccga tggtgcgggc cacgggcccg ttcacgatga cgaccgggcc cgagaaccag
     1021 gtggtggcga ggaggccgtg catgttgaac tcgtcagtgc acgccgcctc caccgaggcg
     1081 agcaccaccg gtaggtagtc gggtcgacag ccggcgagca ccgcgttgat ggccaccttc
     1141 tcgacgctgc actccacgag gtcgggtggc accaccgcca cgacgtcgtc gggggcgcgg
     1201 gtcgtgccgg tgagcatccg ggccacgcgc tccggtgtcg ggggcacgac cgggagaccg
     1261 tcgctccagc cccgctcgaa catggcctcc aggtcgtctt cgctcgctcc cagctcgacc
     1321 cggcgcgacc ccagccgtcg ggccgcggtg gtgtcggcta gggcggccgc gaggtccggg
     1381 tcctgggtcc gcgagccgca cccgggccgg tggggcggga ggcccgcccc gagcccgtcg
     1441 aggccggtga acgcctccca ctggtcgcgg ctccagccga cgaggcgggc ggtttcgcgc
     1501 ccgtcctcga cccgaatcaa cgtggggacg gtgtcgaggt cgagggcgag cgacacggcc
     1561 agcccggtgt cgtcgaccgg gtcgaggtcg ggtgggaacg ccgcgtcgtc ctgggtgtag
     1621 atcgtgagcg gaggggcgcc cgacgcgtca cggagctgcg cgaggaccgg ggtgacgagc
     1681 tggcacgtcg ggcagtcgcg cttgacgatg gcgacgagtc cgtcgggcag cgagacggtc
     1741 actgcagcgc cgcgacgagg tggtcgatgg cgagcgtgag ggtcacgaca tcgtcgagct
     1801 cgatggccgg gaacagcgcg atccgaagct ggttgcgtcc cagcttccgg tacgactcgg
     1861 tgtcgacgat gccgttggtg cgcaacgccg cggccagctg ggtggcgtcg accccatcga
     1921 agtcgatcgt ggcggtcaca tggctgcgct cggcgggctt ggcgacgaat ggcgtcgcgt
     1981 acgggctcgc ctcggcccat ccgtagagga cctccgcggc ccggtcacag cgggtcgcgg
     2041 cccactccag gccgccgtgg gcgaccatcc actcgacggt gtcgaccatg aggaacagcg
     2101 tggtgagcgc gggcgtgttg taggtctggt cctggcggga gttctcgagc gcgatcggca
     2161 agctcaggaa cgccggctgc caccgtccgg acgcgccgat ccgctcgatc cgctcgaccg
     2221 cggcggggga gcacaacgcg aaccacaggc cgccgtcgga tccgaaggcc ttctggggcg
     2281 cgaagtagta gacgtcgaag gcgtcgggat cgacgcgcag gccaccggcg gcggaggtcc
     2341 cgtcgacgag gaccagcccg tcggcctcgg ggcgggcgat gtccatcatc acc
//
LOCUS       DATCCH010000205         2090 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_479197, whole genome shotgun sequence.
ACCESSION   DATCCH010000205 DATCCH010000000
VERSION     DATCCH010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479197"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            285..872
                     /locus_tag="VIJ44_04055"
     CDS             285..872
                     /locus_tag="VIJ44_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018178861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY5165106.1"
                     /translation="MTKSVLYMSMSLDGFITGPGDGPGNGLGTGGDRLHKWLGEPVSE
                     FPHFDPPGLSGQVFAELMATGAVVVGRKTFDYAGHWCGDHHGVPIFVPTRGTPPAPQS
                     GWVHYVPDAATAMREAKAAAVDKEVMVHGAGLAQSLLREGLLDELELHLIPVVLGDGR
                     RLFGADRIEVELTRVLDAPGVTHLRYRVASGAAVG"
     gene            970..1200
                     /locus_tag="VIJ44_04060"
     CDS             970..1200
                     /locus_tag="VIJ44_04060"
                     /inference="COORDINATES: protein motif:HMM:NF019401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEY5165107.1"
                     /translation="MASILLGLEVLIIVDIIRTIVVDPSLQSVGVLGVTVLIRIVLSV
                     SLEAEIDGTWPWNRWRLQSAGQATKTCPDVED"
     gene            complement(1639..>2090)
                     /locus_tag="VIJ44_04065"
     CDS             complement(1639..>2090)
                     /locus_tag="VIJ44_04065"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HEY5165108.1"
                     /translation="TEPDKNNDIHGFLRWRPWQPVDHQPDRVVMAATLFPLQGYPFAL
                     DLRIDYHLDEGGLSVTTTATNIGDQPSPYGCGQHPYLSPGEGLIDDCTLQLEADTRIL
                     TDPDRQLPIGVQSVAGTPYDFRDGTRLGDLKLDFAFTDLHRDTNNRA"
BASE COUNT          325 a          629 c          762 g          374 t
ORIGIN      
        1 ggtccggtca tcgttcctct atcgagaagc ccgcgtggac tgcaccatga gcggcccgtt
       61 gctgggagaa tcagccgcgg aggagcccac gtggctcgca actactcgcg gcccaccatc
      121 aagacctggt tcgcccaagc aaactcgtgc gcttacccgg gatgtgctga accgctcgta
      181 tttgaggtca ggaatgtcac gaccgtcgta gcgcagattg cgcacatcca ttgaacgaat
      241 cttcaagacg aggtcggcgc tacgccggcg aaggaggaga ccgcatgacg aagtcagtcc
      301 tctacatgtc gatgtcgctc gacgggttta tcaccggccc gggcgacggc ccgggcaacg
      361 ggctcgggac cggtggcgac cggctgcaca agtggctcgg cgagcccgtc tccgagttcc
      421 cgcacttcga cccgccgggg ctgagcgggc aggtgttcgc cgagttgatg gcgacgggtg
      481 cggtcgtcgt cggccgcaag acgttcgact acgccggtca ctggtgcggc gaccaccacg
      541 gcgtaccgat cttcgtgcct acgcgtggca ccccgccggc gccgcagagc ggctgggtcc
      601 actacgtgcc cgacgccgcg accgcgatgc gcgaggccaa ggcagccgca gtcgacaagg
      661 aggtgatggt gcacggcgcc ggcctcgcgc agtcgctgct gcgcgagggt ctgctcgacg
      721 agctggagct ccacctgatc ccggtcgtgc tcggcgatgg gcggcggctg ttcggcgcag
      781 accgcatcga ggtcgagctg acgcgcgtgc tcgacgcgcc cggcgtcacg cacctgcgct
      841 atcgagtggc gtcgggagct gccgtaggtt gatcctgctg ctgtcggtct cgatgggcgg
      901 ctccgccggc cgccccacgg gacgatcgac tggcccacag ccccgtccct gaccacggcg
      961 accaacgcca tggcctcgat cttgctcggc ctcgaagtgt tgataatcgt cgacatcatt
     1021 cggaccatcg tcgtcgaccc gtcgctccag agcgtcgggg tgctgggggt gaccgtgttg
     1081 attaggattg tgttgagcgt ctcgctggaa gccgagatcg atgggacttg gccatggaac
     1141 cggtggcgcc tccaatcggc aggccaggca acgaaaacgt gccctgacgt cgaggactga
     1201 gcgcaccggc cacgcccgaa tcgggaagtt cgatcggcag gtgaacgacg cagcccccgg
     1261 gttcgttcgg aacttgagcc gatggtgctc gttacttgac tcgccgcggc gccgagggcg
     1321 tcgtcgtgga gcccacagca tgcaccccga aaagtcgatc agcggggttg ttctatgtgg
     1381 tttcgcagga tccatgacca gcccgagtca gggcgcgagc gaggctcccc acgtcgcgct
     1441 cgtggaagcg ccgggcgcga gttggatcac gcggtcgccg gtctggaacg cgttgggtgg
     1501 gcaagtcatg ggttcggtgc ccaggccgag gcggcgccgg tcggtggaga gcgtgtcgcc
     1561 ggtgtagatc tcgacgattg ggtaggcgcc atcgacccag agctgggcgg cgcggccgtc
     1621 cgtgcgggtg agccggactc aggcccggtt attggtgtct cggtgcaggt cggtgaatgc
     1681 gaagtcgagc ttgaggtcgc cgagtctcgt gccgtcgcgg aagtcgtagg gagtgccggc
     1741 cacgctttgg actcctatgg gcagctggcg gtccgggtcg gtgaggatgc gggtgtctgc
     1801 ttcgagctgg agggtgcagt cgtcgatgag tccttcaccg ggggaaaggt agggatgctg
     1861 tccgcagccg taggggctcg gttggtcgcc gatgttggtg gccgtggtcg tgacgctcag
     1921 gccgccctcg tcgaggtggt agtcgatgcg aaggtcgagg gcgaatggat atccctgtag
     1981 cgggaacagc gtggccgcca tgacgacacg gtcaggttgg tggtcgacgg gttgccacgg
     2041 gcgccagcgc aggaagccgt ggatgtcgtt gttcttgtcc ggttcggtga
//
LOCUS       DATCCH010000206         2234 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_384908, whole genome shotgun sequence.
ACCESSION   DATCCH010000206 DATCCH010000000
VERSION     DATCCH010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384908"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="VIJ44_04070"
     CDS             complement(<1..763)
                     /locus_tag="VIJ44_04070"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEY5165109.1"
                     /translation="MPELPDITILAEAFGAALVGRTIVAASAPDPLVLRATPTEFATL
                     LGDRLLDVRRRGKFLILEAGRTTVWASPMLTGRFAMVAPGVTRPKGSVILRFGPWVGP
                     GPDVVAPWVRDATWLPMRDAEVELRYLDPTRMGKLYLVPVDAPRSVPGTAPADLGPDA
                     NDPALTVAEFRTRIRRHPGELKGLLRNQSFVAGIGNAYSDEILWAARLSPFRRRSSLA
                     GEEVDALYAAMRETLAWSVDTLRARVPPTFEKQVRD"
     gene            991..>2234
                     /locus_tag="VIJ44_04075"
     CDS             991..>2234
                     /locus_tag="VIJ44_04075"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEY5165110.1"
                     /translation="MTEEQQAGTTPEITSEANAPEVAPVAEPATVVADVAAETPEPSM
                     IEPVAEAVAEPVAEAVPAGVSPEDLADAPEAEEPEVALPAPAARPEPSTMEELLAEQD
                     TEIRSFKHGDVVEGAIVRIDKDEILVDIGAKSEGVVSNRELYGRNAESSPVLAVGDVV
                     LVYVLQPESPEGHVVLSLRRAGLERKWRSMQEQFETGVIIEAPVIDHNKGGLIVDCGI
                     RGFVPISQIVDFPRRPQNEQPRDAAQEIAEKLQPYVGRRLRLKILEVNRKANRLILSE
                     KVALYEERREKRDELFSSLQVGQKVKGNIRSIAPFGVFVDLGGIDGLVHKSELSWNKV
                     NNPEAGYKVGEEVEAEVIDINHERGRISLSIRRLQPDPWHSTVADFKVGEIIDGTVTK
                     LVNFGAFVRVRDGLEGLIHISEL"
BASE COUNT          389 a          750 c          779 g          316 t
ORIGIN      
        1 ggtcgcggac ctgcttctcg aaggtgggcg ggacgcgggc tcggagggtg tcgacggacc
       61 acgccagcgt ctcgcgcatc gccgcataca gggcgtcgac ctcttcgccg gcgaggctcg
      121 atcgtcgccg aaacgggctg agtcgcgcgg cccagaggat ctcgtcgctg taggcattgc
      181 cgattcccgc aacgaacgac tggttccgga ggagtccctt gagctcgccc ggatggcggc
      241 ggatccgggt tcggaactcg gcgacggtca gggccgggtc gttggcatcg ggcccaaggt
      301 cggccggtgc cgtgccgggc acgctgcgag gcgcgtcaac cgggaccagg tagagcttgc
      361 ccatccgggt tggatcgaga tagcgcagct cgacctcggc gtctcgcatc gggagccagg
      421 tggcgtcgcg cacccagggc gccacgacgt ccgggcccgg cccgacccac ggcccgaacc
      481 ggaggatcac ggaccctttc gggcgcgtga cgccgggcgc gaccatcgcg aaccgcccgg
      541 tcagcatcgg tgacgcccac acggtggtac ggccggcttc gaggatcagg aacttgccgc
      601 gccgccggac gtccagcagg cgatcgccga ggagggtcgc gaactcggtt ggtgtcgccc
      661 ggagtacgag agggtcgggc gcgcttgccg cgacgatcgt ccgcccgacg agcgcagcgc
      721 cgaaggcttc ggccaggatc gtgatgtcgg gcagctcggg cacggctcgc ggattgtacc
      781 cgccaacgga ttgtacccgc caagagtcgt gtgctagact cgcgcggccc cgcgagggct
      841 cgacagccga acagcttccg accgacggag cggaggagcg gaccgcacgc agggcgcacg
      901 gaccgaacct ggcccaacga cgacggcgcg agcgcggctc ggccgtcgtc ctcgtttcgg
      961 gcggactcca tgacagaggt aagaagaccc ttgaccgaag agcagcaggc agggaccacg
     1021 ccggagatca cgtcggaggc gaatgccccc gaggtggccc ccgtcgcaga accggcgacc
     1081 gtggtcgccg acgtggccgc cgagaccccc gagccgtcca tgatcgagcc cgtggcagag
     1141 gccgtcgccg agcccgtggc agaggccgtc ccggctggcg tgagccccga ggacctcgcc
     1201 gacgcacccg aggccgagga gcccgaggtt gctctccccg cgccggcggc ccggcccgag
     1261 ccctcgacta tggaagagct gctcgcggag caagacaccg agatccggag cttcaagcac
     1321 ggcgacgtcg tcgagggcgc catcgttcgg atcgacaagg acgagatcct cgtcgacatc
     1381 ggggcgaaaa gcgaaggcgt ggtctccaac cgcgagctct acggtcgcaa cgccgaatcc
     1441 tctccagtcc tcgcggtcgg ggatgtagtc ctggtctacg tgctccagcc ggagtcgccg
     1501 gaaggccacg tggtcctctc actccgtcgc gccggcctcg agcgcaagtg gcgctcgatg
     1561 caggagcagt tcgaaacggg cgtcatcatc gaggcgccgg tgatcgacca caacaagggt
     1621 ggcctcatcg tcgactgcgg gattcgcggg ttcgttccga tcagccagat cgtcgacttc
     1681 ccgcgacgtc cccagaacga gcagccccgt gacgccgccc aggagatcgc cgagaagctg
     1741 cagccgtacg tcggccgtcg gctccgcctc aagatcctcg aggtcaatcg gaaggcgaac
     1801 cgcctgatcc tctcggagaa ggtggcgctc tacgaggagc gccgcgagaa gcgcgacgag
     1861 ctcttcagca gcctccaggt gggtcagaag gtcaagggca acatccgatc gatcgcgccg
     1921 ttcggcgtct tcgtcgacct cggtgggatc gacgggctcg tccacaagag cgagctgtcg
     1981 tggaacaagg tcaacaaccc ggaggccggc tacaaggtcg gcgaagaggt cgaggccgag
     2041 gtcatcgaca tcaaccatga gcgcgggcgg atcagccttt cgattcgtcg cctccagccg
     2101 gatccctggc actccacggt ggccgacttc aaggtcggcg agatcatcga tggaacggtc
     2161 accaagctcg tcaacttcgg cgccttcgtt cgagtccgcg acggcctcga gggtttgatc
     2221 cacatctccg agct
//
LOCUS       DATCCH010000207         2599 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_52489, whole genome shotgun sequence.
ACCESSION   DATCCH010000207 DATCCH010000000
VERSION     DATCCH010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52489"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="VIJ44_04080"
     CDS             complement(<1..277)
                     /locus_tag="VIJ44_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017680482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEY5165111.1"
                     /translation="MTSPRDIGARDRIRDAHDASLFVEAGAGTGKTSALVDRVVALVA
                     CGAVELRHVAAITFTEAAAAELRDRVRTALETVANGRDELFDDDDARR"
     gene            complement(274..>2599)
                     /locus_tag="VIJ44_04085"
     CDS             complement(274..>2599)
                     /locus_tag="VIJ44_04085"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEY5165112.1"
                     /translation="LARAEAGAPFHRLAILYTVPDPYARLVPELLDSAEIPWTGPSPR
                     PLGDSVAGRVLTGVLDLIHADFARDAVAAWLGSGPILDGSGHRVPGTRWDLVSREAGI
                     VGGGDQWRDRLALRRRELDNKLAQLDVDDEPEWRVGRLERDRDQVIALERFVHELLDQ
                     ARVPVPTSWSALSGWARGLLARYLGGEGRRGDWPERELDAARRLESALEDLAGLDELG
                     TEVDLARFRDALEAALESSTTHVGRFGTGVFVGPLHAAVGTDFATIAVVGGFEGALPP
                     RGRDDPFLPDDDRSVVGTLVTTAERRAESRRAYLAALAAADETMLCFPRADPRAQQGR
                     LPARWLLETASAHAGRAVTAEELRNLDPQPWLDVVASFEQGVTSDTEPGSLTERDLRS
                     LCEWRNAHRRLVDHPLAHGSLGRGFALAARRSSDRFTAFSGNVGASTLVAHGPDRPIG
                     ATSFEDWAQCPFRYLLGRVLRVREVARPEATETISALDEGTLVHAILEAFVREQPPRS
                     PTYRWTDADRARMLQIVARHCDDAEARGITGRSLLWRLARRRIAGTAVRFLTVDTEFR
                     EALDTIPTAAGLEVDFGMAGQPGVTVELSGDRSVTFRGRIDRVDRSPDGARVVVYDYK
                     TGRPRDDGLEDDPVCAGQRLQLPIYGLAAASDRDDEEVHAYYWYTRAETLEKARKGYR
                     LDDSVRSRFVDVLDTIVDGIDHGCFPAYPGVRDYDPRVQRETFQNCLWCPYDRLCPLD
                     RGTAWEHQSSDEAMQPFWRLELSDDPGDDGDGRP"
BASE COUNT          374 a          874 c          976 g          375 t
ORIGIN      
        1 gtcgtcgtgc gtcgtcgtcg tcgaagagct cgtcgcgacc gttcgcgacg gtctccagcg
       61 cggtacggac tcggtcgcgc aactcggccg cggcggcttc ggtgaaggtg atcgcggcga
      121 cgtgacgcag ttcgaccgcg ccacatgcga cgagcgcgac cacccggtcg acgagggcac
      181 tcgtcttgcc ggttcccgcc ccggcctcga cgaagaggct cgcgtcgtgg gcgtcgcgga
      241 tgcggtcccg ggccccgatg tcgcgcgggg aagtcacggc cgaccatcgc catcgtcgcc
      301 cgggtcgtcg ctgagctcga ggcgccagaa cggctgcatg gcctcgtcgg acgactgatg
      361 ttcccaggcg gtgcctcgat cgagcgggca gagacggtcg tacgggcacc acaggcagtt
      421 ctggaaggtc tcgcgctgaa cgcgagggtc gtagtcgcgc acgccgggat acgcggggaa
      481 gcatccgtgg tcgatgccgt cgacgatggt gtcgaggacg tcaacgaagc gggaccggac
      541 cgagtcgtcc agccggtagc ccttgcgggc cttctcgagc gtctcggctc gggtgtacca
      601 gtagtaggcg tgcacctcct cgtcgtcgcg gtcgcttgct gcggcgagcc cgtagatcgg
      661 gagctgcagg cgctgaccgg cgcaaaccgg atcgtcctcg agaccgtcgt ctcggggccg
      721 tcccgtcttg tagtcgtaga cgacgacgcg cgccccgtcg ggagaccggt cgacccggtc
      781 gatgcggccc cggaaggtca cgcttcggtc acccgagagc tccacggtga cgcccggttg
      841 gccggccatg ccgaagtcca cttcgaggcc ggcggcggtc ggaatggtgt ccagcgcttc
      901 gcggaactcg gtgtcgacgg tcaagaaccg aaccgcggtg cccgcgatac ggcgtcgggc
      961 caggcgccac agcagcgatc ggcccgtgat tcctcgcgct tcggcgtcgt cgcagtgtcg
     1021 ggcgacgatc tggagcatcc gggcccggtc cgcgtcggtc cagcgatacg tcggtgatcg
     1081 tgggggctgt tcccgcacga acgcctcgag gatggcgtgc accaacgtcc cctcatcgag
     1141 cgcgctgatg gtctccgtgg cctccggtcg cgccacctct cggacccgaa gcacccggcc
     1201 gaggagatac cggaacgggc actgggccca gtcctcgaag gatgtggcac cgatcggccg
     1261 gtcgggcccg tgggcgacga gcgtcgaggc gcccacgttg ccgctgaacg cagtgaaccg
     1321 gtccgatgac cgcctggcgg cgagggcgaa gccgcgccct aacgaaccat gggcgagcgg
     1381 gtggtcaacg agacgacggt gcgcgttgcg ccactcgcag aggctgcgca ggtcccgctc
     1441 ggtgagcgat ccgggctcgg tgtctgacgt gacgccctgc tcgaaggagg cgacgacatc
     1501 gagccagggc tgtgggtcga ggttgcgcag ctcctcggcg gtcaccgccc gcccggcatg
     1561 agcgctcgcc gtctcgagca gccaccgtgc tgggagccgg ccctgttgcg cccgcgggtc
     1621 ggcccgaggg aagcagagca tggtctcgtc ggcggcagcg agcgcggcga ggtaggcgcg
     1681 gcggctctcg gcccggcgct cggccgtggt gaccagggtc ccgaccaccg atcggtcgtc
     1741 gtcggggagg aacgggtcgt cgcggccccg tggagggagg gcaccctcga agcctccgac
     1801 gaccgcgatg gtcgcgaagt cggtcccgac cgcggcgtgc agcgggccga cgaagacccc
     1861 ggtgccgaac cggccaacat gggttgtcga gctctccaac gcggcttcga gtgcgtcacg
     1921 gaaccgggcc aggtcgacct cggttccgag ctcgtcgagg ccggcgagat cttcgagcgc
     1981 ggattcgagt cgtcgggccg cgtcgagctc tcgttccggc cagtcgccgc gccggccttc
     2041 gccgccgagg taccgggcga gcaggccccg agcccatccg ctcagcgcgg accagctcgt
     2101 cgggaccggc acccgagcct gatcgaggag ctcatggacg aaacgctcga gggcgatcac
     2161 ctggtcgcgg tcccgttcga ggcgaccgac gcgccattcg ggctcgtcgt cgacatcgag
     2221 ctgggccagc ttgttgtcga gctctcggcg gcggagtgcg agccggtcgc gccactggtc
     2281 cccgccaccg acgatgccgg cctcgcggga cacgaggtcc catcgggtgc cgggcacccg
     2341 atgtcctgac ccgtcgagga tcgggcctga tccgagccag gcggccacgg cgtcgcgggc
     2401 gaaatccgcg tggatgaggt cgagcacccc ggtgaggact cggccggcga cgctgtcacc
     2461 caacgggcgg ggtgacgggc cggtccaggg aatctcggcg gagtcgagga gctccggtac
     2521 gagacgcgcg tacgggtcgg ggaccgtgta gaggatcgcg aggcgatgga acggggcacc
     2581 ggcttcggcg cgggcgagg
//
LOCUS       DATCCH010000208         2975 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_135558, whole genome shotgun sequence.
ACCESSION   DATCCH010000208 DATCCH010000000
VERSION     DATCCH010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135558"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="VIJ44_04090"
     CDS             complement(<1..367)
                     /locus_tag="VIJ44_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HEY5165113.1"
                     /translation="MTTGQHQHEHLHRAPRDCPVCSTRLDLTRLGCPSCGTELSGGFV
                     QCAYCGLSDEDLDMLRVFLASRGNMKDLERHLGVSYPTARQRFADLLGRLGFEDQAVA
                     VDREAVLADLAAGRITVDQA"
     gene            complement(364..1275)
                     /locus_tag="VIJ44_04095"
     CDS             complement(364..1275)
                     /locus_tag="VIJ44_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165114.1"
                     /translation="MTDDLRALLESVAEGRIAPARAAQLIDDLPSETPTETPTQAPTQ
                     TPAETSSRAAAAPAAETPAAPTAPAAPTAAGPPSTESADTRVERVRITASARPVRLVG
                     DHTVDTVAVDGPHSVRREGGTIRVDATPLGGAASDVPGSYRYERKTGFSRWLGQTSLV
                     GVPLTVRVHPSLAAEAEVMAGSLEIVGMSGPVTFSVTAGTVRAQDCTGPFTGSVRAGS
                     ARLEIRPTAGHSLVRVESGSLDLRLQPGSDVRVTGRVDLGEIKVRAADGTQRIINREE
                     LKDVVVGAGTGTFELEVAMGSAKVRLP"
     gene            complement(1574..2782)
                     /gene="ilvA"
                     /locus_tag="VIJ44_04100"
     CDS             complement(1574..2782)
                     /gene="ilvA"
                     /locus_tag="VIJ44_04100"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HEY5165115.1"
                     /translation="MTITLADIEAARDLLVGIARRTPMEGSRPLSDAVGGPVHFKCEN
                     LQRAGSFKIRGAYTRMSRLSAEERAAGVVAASAGNHAQGVALAASMLDIRTTVFMPNG
                     APIPKVLATRGYGADVRFAGTNLDEALVDARAFSAETGAVLIHPFDHPDIVAGQGTCG
                     LEILEQVPDVKTVLVCTGGGGLLAGIALAVKSSRPDVRVIGVQAARAAAYPASLAAGH
                     PVKLAEMSTMADGIAVGLPGEVPFELVREYVDEIRTVSEESLSRALLLCLERAKLVVE
                     PAGAAAVAAILDDPLAFEPPVVAVLSGGNIDPLLMLRVLRYGLVAAGRFLTLTVRVPD
                     RPGNLAALLSMLARFDANVLDVGHARISGSLGLHEVDIAIELETRGEPHADEVLARLA
                     EEGYAVTRLR"
     gene            complement(2796..>2975)
                     /locus_tag="VIJ44_04105"
     CDS             complement(2796..>2975)
                     /locus_tag="VIJ44_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012851426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165116.1"
                     /translation="PLLFGPLEDALRRYARLSFAQSVRVVPATLGQDAGLVGAAGLVL
                     AADTYWNPLPGDGER"
BASE COUNT          441 a         1035 c         1089 g          410 t
ORIGIN      
        1 cggcctggtc gaccgtgatc cgtccggcgg ccaggtcggc caagacagcc tcccggtcga
       61 ccgccaccgc ttggtcctcg aacccgagcc ggccgagcag gtcggcgaac cgctgcctcg
      121 cggtggggta gctcacgccg aggtggcgct cgaggtcctt catgttgcca cggctggcga
      181 ggaacacccg gagcatgtcc agatcctcgt cggacagccc gcagtaggcg cactgaacga
      241 agccgccgga gagctcggtc ccgcagctcg ggcagccgag gcgggtgagg tcgagcctcg
      301 tcgagcagac cgggcagtcg cgcggggcgc ggtgcaggtg ctcgtgctgg tgctggccgg
      361 tcgtcatggc agccgcacct tcgccgagcc catggccacc tcgagctcga acgtgccggt
      421 gccggccccc acgacgacgt ccttcagctc ctcacggttg atgatccgct gggtgccgtc
      481 ggcggcccgt accttgatct cgcccaggtc gacccgtcca gtgacacgca cgtcggagcc
      541 gggctgcagc cgcaggtcca ggctgcccga ctccactcgt accagcgagt ggccggcggt
      601 gggccggatc tccagcctgg cgctgccggc gcggacggat ccggtgaacg gccccgtgca
      661 gtcctgggca cgtacggtgc ccgcggtgac cgagaaggtc accgggccgc tcatgcccac
      721 gatctccagg gacccggcca tcacctcggc ctcggcagcg agcgagggat gaacgcgtac
      781 ggtcagcggg accccgacca gcgaggtctg gccgagccac cgggagaagc cggtcttgcg
      841 ctcgtaccgg tacgagccgg ggacgtcgga ggcggcgccg ccgagcggcg tcgcatcgac
      901 gcggatcgtg cccccctcgc gccgtaccga gtgcggcccg tcgacggcga cggtgtccac
      961 cgtgtggtcg ccgaccaggc ggaccgggcg ggccgaggcg gtgatccgca cccgctcgac
     1021 ccgtgtgtcg gcgctttccg tcgatggcgg gccggcggca gtgggcgctg ccggtgcagt
     1081 cggtgccgcg ggtgtctcgg ccgccggcgc tgcggcggct cggctggacg tctctgcggg
     1141 cgtctgggtg ggcgcctgtg tgggcgtctc ggtgggcgtc tcggacggta ggtcgtcgat
     1201 cagctgagct gcccgggcgg gcgcgatccg cccctcggcg acactttcga gcaaagcgcg
     1261 caggtcatcg gtcatgacgc aaagtagaaa cccactctta cctaaatgtc aaatctcgat
     1321 ctcaaaagcg aaacccgcac ctacagcagc gcctcctcgg cgggcttcct ggggcacgtg
     1381 tcgtctggtg gggtcaggac accggtagac gacacgtgct cgagatcggc gcggatcggg
     1441 gttgccagag caaccgcaga tcgcgccacc cccgatgccg agccccaatc cgaggcggaa
     1501 tgggtgggtt tggggtgttg tcgtagctgc gccgttgacg ttgacgttga cgttgatctc
     1561 gatggcaccc cactcaccga agccgggtga ccgcgtagcc ctcctcggcc agccgggcga
     1621 gcacctcgtc ggcgtgcggc tcgccacggg tctcgagctc gatggcgatg tcgacctcgt
     1681 gcagcccgag agatcccgag atgcgggcgt ggccgacgtc gagcacgttg gcgtcgaagc
     1741 gcgcgagcat cgagaggagg gcggccaggt tccctgggcg gtcgggcacc cgtacggtca
     1801 gggtcaggaa ccgcccggcg gcgacgagcc cgtaccgcag cacccggagc atcagcagcg
     1861 ggtcgatgtt gccgccggac agaaccgcga cgaccggcgg ctcgaacgcc agggggtcgt
     1921 cgaggatggc ggccaccgca gccgcaccgg ccggctccac gacgagcttg gcgcgctcga
     1981 ggcagagcag caaggcccgg gagagcgact cctcggagac ggtacggatc tcgtcgacgt
     2041 actcgcggac cagctcgaaa ggcacctccc ccggcagtcc cacggcgatg ccgtcggcca
     2101 tggtggacat ctcggcgagc ttcaccgggt ggccggccgc gagcgacgcg ggataggccg
     2161 cggcccgggc ggcctgcaca ccgatgacgc gtacgtccgg tcgcgacgac ttgaccgcga
     2221 gggcgatgcc ggccagcagc ccgccacccc ctgtacacac gaggacggtc ttgacgtcgg
     2281 gcacctgctc gaggatctcg aggccgcagg tcccttgccc ggcaacgatg tccgggtggt
     2341 cgaacgggtg gatgagcacg gcgcccgtct ccgccgagaa cgcccgcgcg tcgacgagcg
     2401 cctcgtcgag gttggtgccg gcgaagcgca cgtcggcgcc gtagccgcgc gtcgccagga
     2461 ccttggggat cggtgcaccg ttcggcatga acacggtcgt acggatgtcc agcatcgacg
     2521 ccgcgagggc gaccccctgc gcgtggttgc cggcactggc agccaccaca ccggcagcgc
     2581 gctcctccgc ggacagccgg gacatccgcg tgtaggcgcc gcggatcttg aacgaaccgg
     2641 cccgttgcag gttctcgcac ttgaagtgga ccggaccgcc cacggcgtcg gacagcgggc
     2701 gcgaaccctc catgggggta cggcgggcga tgcccacgag caggtctcgg gcggcttcga
     2761 tgtcggcgag cgtgatcgtc atgccccgga gtctgtcagc gctccccgtc gcccgggagg
     2821 gggttccagt aggtgtcggc ggccaggacg aggccggctg caccgacgag cccggcgtcc
     2881 tgcccgagcg tggccggcac gacccggacc gactgcgcga acgacagccg cgcgtagcgc
     2941 cggagcgcgt cctccaacgg cccgaacagc agcgg
//
LOCUS       DATCCH010000209         2824 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_52615, whole genome shotgun sequence.
ACCESSION   DATCCH010000209 DATCCH010000000
VERSION     DATCCH010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52615"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /gene="phoU"
                     /locus_tag="VIJ44_04110"
     CDS             <1..749
                     /gene="phoU"
                     /locus_tag="VIJ44_04110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEY5165117.1"
                     /translation="RAAAAQAMMRDVTIMGSSDEARKAFAEELLDLRADVIRLAALVT
                     EAIAAGTQALLDADLSAAEHVVEQDAGIDELAYGIEDHCFVLLARQQPMASDLRTVLA
                     VLRISHELERSGDLMKSVVKATRQLYPHSIDPKLRGIISRMGEQAANETRLAIDAFAD
                     SDPSRAAALFDMDDVMDDLTKSLFRYIMNREGPADEGRLLESIQLALVGRHYERIADH
                     AVNVADRVVFIATGLHGAGDSEDFSPGADT"
     gene            complement(771..1052)
                     /locus_tag="VIJ44_04115"
     CDS             complement(771..1052)
                     /locus_tag="VIJ44_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165118.1"
                     /translation="MAMNQEELEQLPPQGHVKLMYLGPVAPHWEVHGVFGDGRVIDEF
                     RQRALARLQLLPPHDPQFRRNRERVNRDAERERLILDWDLGYPEDDATR"
     gene            complement(1091..2365)
                     /locus_tag="VIJ44_04120"
     CDS             complement(1091..2365)
                     /locus_tag="VIJ44_04120"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY5165119.1"
                     /translation="MADEPSRDDTPPRASADLPPDVGATGPQAVSVTPPAPDATAPDL
                     EPSTAPTSGNPGGGSSNRPQGLRQALLGALVGGLVGALVAGGIVYAVDDNGATSVTKI
                     VQSNPAAVTVRPSTQIAAPSDIAGIVAKAEPAIVAVTTDGGPNSGNGGAGTGFVISSD
                     GYIATNNHVVEGASKIQVQLTSGRIVDATTVGRDPSTDLAVIKANATGLPAVVLGNSD
                     SPNVQIGDSVVAIGNALALEGGLSVTNGIISGVNRQVPEQNGAVLFGMLQTDAAINPG
                     NSGGPLLDSQARVIGINTAVAGQAQNIGFAIPISQAIPVFNDLRSGRKPAFLGVSTTD
                     LTPSIASQLNVSVTAGAVITQVSSNTPASSAGLKKDDVIVEIGSTAITGSADVQTAVR
                     SHQPGDAVPIVIVRGADRQTVSAKLTQRPNLG"
     gene            2552..2812
                     /locus_tag="VIJ44_04125"
     CDS             2552..2812
                     /locus_tag="VIJ44_04125"
                     /inference="COORDINATES: protein motif:HMM:NF014520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEY5165120.1"
                     /translation="MDELPRPDHHTPSWMLHARCREAAPGSFFPSDGVGVDRARRVCG
                     DCPVKAECLEYALEFRIDHGVWGGCSERERRRILRQRRLATR"
BASE COUNT          424 a          964 c          996 g          440 t
ORIGIN      
        1 cccgggctgc ggcggctcag gcaatgatga gggacgtgac gatcatgggg agcagtgacg
       61 aagctcgcaa ggccttcgcc gaggaactcc tggacctacg ggccgatgtc atccgccttg
      121 cggccctcgt gaccgaggcg atcgcggccg gaacccaggc gctcctcgac gccgacctca
      181 gcgccgcgga gcacgtcgtc gagcaggacg ccggcatcga cgagctggcg tatggcatcg
      241 aggaccactg tttcgtcctc ctcgctcgcc agcagccgat ggcgagcgat ctgcggacgg
      301 tgttggcggt cctgcggatc agtcacgagc tggaacgcag cggcgacctc atgaagagtg
      361 tggtgaaggc cacccgccag ctctacccgc acagcatcga cccgaagttg cggggaatca
      421 tcagccggat gggtgaacag gcggccaacg agacccggct ggcgatcgac gcgttcgccg
      481 acagcgaccc gtcgcgtgcc gcggcgctgt tcgacatgga cgacgtgatg gacgacctca
      541 ccaagagcct gttccgctac atcatgaacc gggaggggcc cgccgacgaa ggacgactcc
      601 tcgaatcgat ccagctggcg ctggtcggcc gtcactacga gcggatcgcc gatcatgccg
      661 tgaacgtcgc cgaccgggtg gtgttcatcg ccaccgggct ccatggtgcc ggcgacagcg
      721 aggacttcag tcccggcgcc gacacctgac gccgaccgga gcagcccgga tcagcgggtg
      781 gcgtcgtcct cggggtatcc gaggtcccag tcgaggatca ggcgctcccg ctccgcgtca
      841 cggttgaccc gctcccggtt gcgccggaac tggggatcat gcggtgggag gagctgcagc
      901 cgggcgaggg cccgctggcg gaactcgtcg atgacccgcc cgtcgccgaa caccccgtgg
      961 acctcccagt ggggcgccac aggaccgagg tacatcagct tgacgtgccc ttgcggcggg
     1021 agctgctcga gctcctcctg gttcatcgcc atcgcgccag cctaccgggc gccctttgcg
     1081 ccacctcagg tcagcccagg ttgggccgtt gcgtcagctt cgccgagacg gtctggcggt
     1141 ccgcgccgcg cacgatgacg atcggcaccg cgtctccggg ctgatgggac cgaaccgcgg
     1201 tctgcacgtc ggctgagccc gtgatagcgg tcgagccgat ctcgacgatg acgtcgtcct
     1261 tcttgagccc cgcggacgac gccggggtgt tgctgctgac ctgggtgatg accgcgcccg
     1321 cggtgaccga gacgttgagc tggctggcga tcgacggcgt caagtcagtg gtgctcaccc
     1381 cgaggaacgc cggcttgcga ccggaccgca gatcgttgaa caccgggatc gcctgcgaga
     1441 tcgggatcgc gaacccgatg ttctgggcct gacccgcgac ggcggtgttg atgccgatga
     1501 cccgggcctg tgaatcgagg agcggccccc ccgagttgcc agggttgatc gcggcgtcgg
     1561 tctggagcat cccgaagagg acggcgccgt tctgttcggg cacctgacgg ttcacacccg
     1621 agatgatccc gttcgtaacg ctcaacccac cttcgagtgc gagcgcgttc ccgatcgcca
     1681 ccaccgagtc gccgatctgg acgttcgggg aatcggagtt cccgagcacg acggccggga
     1741 ggccggtggc gttggccttg atcacggcca ggtcggtcga gggatcacgc ccgacggtgg
     1801 tggcgtcgac gatgcggccc gacgtgagct gcacctggat cttgctggcc ccttccacca
     1861 catggttgtt ggtggcgatg tagccgtcgg aggagatcac gaagccggtg cccgcaccgc
     1921 cgtttccgct gttcggaccg ccgtcggtcg tgacggccac gatcgccggc tcagccttgg
     1981 cgacgatccc ggcgatgtcg ctgggggccg cgatctgggt ggacgggcgg accgtcaccg
     2041 cggccgggtt cgactgcacg atcttggtga ccgacgtcgc gccgttgtcg tcgacggcgt
     2101 agacgattcc gcccgcgacc aacgcgccga ccagaccgcc gacgagagcg ccgagcagcg
     2161 cctggcgcaa cccctggggt cggttcgacg agccgccgcc cgggttcccc gacgtcggtg
     2221 ccgtcgacgg ctccaagtct ggcgccgtcg cgtcgggagc aggcggcgtg accgagacgg
     2281 cctgcggacc agtcgcgccg acgtccggcg ggaggtcggc ggatgcccgg ggcggggtgt
     2341 cgtcccggct aggttcgtca gccatggcgc tctcctcagg acacggacgg gtgggggaac
     2401 acgacccttt gatccgacgc tctgctcgcc gaccgggttc gggaggatta tcggtcagcc
     2461 cgagcccgag caccggtcag ggcctcgacg gcctcccccc taaccggacg gcgacgggga
     2521 accgacgccg gtccgcgtcc gtttgactgg catggacgag ctcccccgcc ccgatcacca
     2581 caccccgtcg tggatgctcc acgcccgatg ccgggaagcc gccccgggtt cgttcttccc
     2641 gtccgacggg gtgggcgtcg accgggcccg gcgggtctgc ggcgactgtc ccgtgaaggc
     2701 cgagtgcctc gaatacgcat tggagttccg gatcgatcac ggggtgtggg gcggctgctc
     2761 cgaacgcgag cggcgccgca tcctgcgcca gcggcgcctc gccacccgct gagggctatt
     2821 ccgc
//
LOCUS       DATCCH010000210         2160 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_105996, whole genome shotgun sequence.
ACCESSION   DATCCH010000210 DATCCH010000000
VERSION     DATCCH010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105996"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..954
                     /locus_tag="VIJ44_04130"
     CDS             <1..954
                     /locus_tag="VIJ44_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013872182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HEY5165121.1"
                     /translation="ALLRPGRFDRQIVVDRPDLAGRKAILAVHATEKPLAPGVELDLI
                     ARRTPGFTGADLANLLNEAALLSARHGLKLIGLPQLEEATERVMAGPERRSRVISDRE
                     RQIIAYHEGGHALVAHALPHTDPVHKISIIPRGRALGYTLTLPTEDKFLVTRDELIDE
                     LAMLLGGRSAEELVFEDPTTGAQNDIDRATTIARQMVTEFGMSDELGPLRLGQPQGEV
                     FLGRDFAATPDYSDDLAARIDAEVHRLVEHAHTVARAIIQTNRAVLDRLAAELVERET
                     CDVERVREIFSPVEPFAGAGLGRPRAAAASEVAAPNRRARR"
     gene            951..1553
                     /gene="folE"
                     /locus_tag="VIJ44_04135"
     CDS             951..1553
                     /gene="folE"
                     /locus_tag="VIJ44_04135"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEY5165122.1"
                     /translation="MSEPTNGPEVDTGRIEKAVREILEAIGEDPDRDGLQHTPERVAR
                     MYAEIFGGLAEDPAQHLAVTFEADHDEMVLVRDIPLYGICEHHLVPFAGRAHVAYIPG
                     PDGRITGLSKIARLVEGYARRPQVQERLTTQIADALVEVLKPDGVLVMMEAEHLCMSM
                     RGVKKPGALTLTSAVRGLFKTNPATRAEALSLITAPVNRF"
     gene            1711..>2160
                     /gene="folP"
                     /locus_tag="VIJ44_04140"
     CDS             1711..>2160
                     /gene="folP"
                     /locus_tag="VIJ44_04140"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEY5165123.1"
                     /translation="MGIVNVTPDSFSDGGQYLDEDAAVAHGHALAAAGASLLDVGGES
                     TRPGAQPVDEAEERRRVVPVVERLAGETGVPVSIDTTKPTVAAAALDAGAVVVNDVSA
                     GRFHPALLEVVADRGAGYIAMHMLGEPRTMQHDPRYADVVTAVGDFLL"
BASE COUNT          341 a          863 c          675 g          281 t
ORIGIN      
        1 gcgctgttgc gccccggccg cttcgaccgc cagatcgtcg tcgaccgtcc cgacctcgcg
       61 ggccgcaagg cgatcctcgc ggtccacgcg accgagaagc ccctcgcccc cggagtcgag
      121 ctggacctga tcgcccgtcg aacccccggc ttcaccggcg ccgacctcgc caacctcctc
      181 aacgaagcgg cgctgctctc ggcccgtcac ggcctgaagc tcatcggtct cccccagctc
      241 gaggaggcca ccgagcgggt gatggccggc cccgaacggc gcagccgcgt catctccgac
      301 cgggagcgtc aaatcatcgc ctaccacgag ggtggccatg ccctcgtcgc gcacgcgttg
      361 ccccacaccg acccggtgca caagatctcg atcatcccga gaggccgcgc gctcggctac
      421 accctcaccc tcccgaccga agacaagttc ctcgtcaccc gcgacgagct gatcgacgag
      481 ctggcgatgc tcctcggcgg ccgcagcgcc gaagagctcg tgttcgagga cccgacgacc
      541 ggagcccaga acgacatcga ccgggcgacg accatcgccc gccagatggt caccgagttc
      601 ggcatgagcg acgagctcgg cccgcttcgc ctcggccagc cccaaggcga ggtgttcttg
      661 ggtcgcgatt tcgccgccac ccccgactac tccgacgacc tcgcggcccg gatcgacgcc
      721 gaggtgcatc gcctcgtcga gcacgcccac accgtcgccc gagcgatcat ccagaccaac
      781 cgggcggtgc tcgaccggct ggccgccgag ctggtcgaac gagagacgtg tgacgtcgaa
      841 cgagtccggg agatcttctc gccggtcgag ccgttcgcgg gggcaggact cgggcggccc
      901 cgcgcggccg cggcgtccga ggtcgcggcc cccaaccgga gggcgagacg atgagcgaac
      961 cgacgaacgg acccgaggtc gatacgggcc gcatcgagaa agcggtccgc gagatcctcg
     1021 aagccatcgg cgaagacccc gaccgtgacg gcctgcaaca cacccctgag cgcgtggccc
     1081 gcatgtacgc cgagatcttc ggcggcttgg ccgaagaccc ggcccagcat ctcgcggtga
     1141 ccttcgaagc cgaccacgac gagatggtcc tcgtccgcga catccctctc tacggcatct
     1201 gcgagcacca tctcgtcccc ttcgccggcc gggcccacgt ggcctacatc ccgggccccg
     1261 acggacggat caccgggctc tccaagatcg cccgcctggt cgagggctac gcccgtcgac
     1321 cccaggtcca agagcggctc accacccaga tcgcggacgc gctcgtcgaa gtcctcaagc
     1381 ccgacggagt gctggtgatg atggaagccg agcatctctg catgtcgatg cggggcgtga
     1441 agaaaccggg cgcgttgacc ctcacctccg cggtgcgggg cttgttcaag accaaccctg
     1501 cgacccgcgc cgaggcgctg agcctcatca ccgcgcccgt caaccggttc tgacccggcg
     1561 accccgggcc tcgacgccgg tccggagcca cccgcgccgg gccatgccgc cccgaacatc
     1621 cttccgaccc ccggcgcagc ccgatccagg cgccagtacc atccggccgt gttcacctgg
     1681 gcgagcctgt gcgcggaacg ccccgcggtg atgggaatcg tcaacgtcac ccccgactcg
     1741 ttctccgacg gcggccagta cctcgacgag gacgccgccg tcgcgcacgg tcacgccctc
     1801 gccgccgccg gtgccagcct gctggacgtc ggcggtgaat cgacccgccc cggcgcgcag
     1861 ccggtcgacg aggccgagga gcgccgccgc gtcgttcccg tcgtcgaacg cctcgccggc
     1921 gagaccggcg tccccgtcag catcgacacc accaagccga ccgtcgccgc ggcggcgctc
     1981 gacgccggcg ccgtcgtcgt caacgacgtg tccgcggggc ggttccaccc cgcgctcctc
     2041 gaggtcgtcg ccgaccgcgg cgcgggctac atcgccatgc acatgctcgg cgagccccgc
     2101 accatgcagc acgacccccg ctacgccgac gtcgtcaccg ccgtcggtga cttcctcctc
//
LOCUS       DATCCH010000211         2050 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_521104, whole genome shotgun sequence.
ACCESSION   DATCCH010000211 DATCCH010000000
VERSION     DATCCH010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521104"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="VIJ44_04145"
     CDS             <1..767
                     /locus_tag="VIJ44_04145"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="catalyzes the formation of
                     5-O-(1-carboxyvinyl)-3-phosphoshikimate from
                     phosphoenolpyruvate and 3-phosphoshikimate in tryptophan
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEY5165124.1"
                     /translation="AGLQAAGSTEIVEPVPSRDHTERMLAALDAPVERTGATSVRVKA
                     GEPRPFELDVPGDPSSAAFFVVATVVTPGSELTVEGVDLNPTRLGFVEVLRRMGADLE
                     VRVREDRLGEPVGDLVARAGPLTGTVVGGDEIPNVQDEVPVLAVAAAFADGITEFRDV
                     AELRVKESDRIATVEAGLGRLGVGVESAPDRLVVRGGHPEPGVWDSHGDHRVAMAGAV
                     AANAVAGASTVRGWASVASSYPRFADDLAACTRPGR"
     gene            764..1429
                     /gene="cmk"
                     /locus_tag="VIJ44_04150"
     CDS             764..1429
                     /gene="cmk"
                     /locus_tag="VIJ44_04150"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEY5165125.1"
                     /translation="MSTVVAIDGPSGAGKSTAARRVASELALEVLDTGAMYRAVTLAV
                     LEAGVDPTGADACAAVAARVLIEQRGPQTLLDGRNVSVEIRSPPVNATVSTVSAHPAV
                     RRVLVDHQRSWVTTRRGGVVEGRDIGTVVFPDAVVKIFLTASETERARRRRAEAAPGS
                     PVSVEAVREDLGRRDRLDSTRAASPLVAADDAIRIDTTGRPIDDVVREIVKQVRARTG
                     ASR"
     gene            1441..>2050
                     /locus_tag="VIJ44_04155"
     CDS             1441..>2050
                     /locus_tag="VIJ44_04155"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY5165126.1"
                     /translation="MVRTVVVGFCATIFRVRLIGRDHVPDSGAYVIAPSHRSIIDVPF
                     TAFVTRRRIRFLAKQELFSSRIGSALFPRLGGIPVERGTTDRAALRACDAVLAEGDPL
                     AIFPEGTRRHGPELGDLFHGVAYLALKRRVPIVPVGIGGSEEILPRGHVRPRPHRVVV
                     VVGEPIVADPSASARRRSDVAALTATLATRLQACLDDARRRAG"
BASE COUNT          239 a          734 c          782 g          295 t
ORIGIN      
        1 tggccgggtt gcaggccgcc ggctcgaccg agatcgtcga gccggtcccg agccgggatc
       61 acaccgagcg gatgctcgcc gcgctcgacg cgccggtcga gcgcaccggg gcgacgagcg
      121 tgcgggtcaa ggccggcgaa ccgcgcccgt tcgagctcga cgtgcctggt gacccgtcgt
      181 cggcggcgtt cttcgtcgtc gccacggtcg tgacccccgg gtcggagctc accgtggaag
      241 gcgtcgatct gaacccgacc cggctcgggt tcgtggaggt gctccgccgg atgggcgccg
      301 acctggaggt ccgggtgcgc gaggaccggc tcggcgagcc ggtcggtgac ctcgtggcgc
      361 gggccgggcc cctcacgggg accgtcgtcg gtggcgacga gatccccaac gtgcaagacg
      421 aagtcccggt gctcgcggtc gcggcggcgt tcgccgacgg gatcaccgag tttcgcgacg
      481 tcgccgagct gcgggtgaag gagagtgacc ggatcgccac cgtggaggcc ggcctgggcc
      541 ggctcggggt gggcgtggag tccgcgcccg accggctcgt ggtgcgcggc ggtcatcccg
      601 agccgggcgt gtgggacagc cacggcgatc atcgggtggc gatggccggg gccgtcgccg
      661 cgaacgcggt ggcgggggcg tcgaccgtgc gaggctgggc ctcggtggcc agctcctacc
      721 cgcgcttcgc cgacgacctc gcggcctgca cccgtcccgg ccggtgagca ccgtcgtcgc
      781 catcgacggc ccgtcgggcg cggggaagtc gacggcggcg cgccgcgtgg cctccgagct
      841 cgccctcgag gtcctcgaca ccggcgctat gtaccgggcc gtgaccctcg cggtcctcga
      901 ggccggagtc gacccgaccg gcgcggacgc gtgcgcagcc gtcgcggcgc gagtgctgat
      961 cgagcagcgc ggcccccaga ccctcctcga cggacggaac gtcagcgttg agatccgctc
     1021 acccccggtc aacgccacgg tgtccacagt gtcggcgcac ccggcggtgc ggcgcgtcct
     1081 cgtcgaccat cagcggtcgt gggtcaccac gcgtcgcggc ggcgtcgtcg aaggccgcga
     1141 catcgggact gtggtgttcc ccgacgcggt cgtcaagatc ttcctcaccg cgtccgagac
     1201 cgagcgggcc cgtcgccggc gcgccgaagc ggcccccggc tccccggtga gcgtcgaggc
     1261 ggtccgggag gacctggggc gacgcgaccg actcgactcg acgcgcgccg cgtcgcccct
     1321 cgtcgccgcc gacgacgcga tcaggatcga caccaccggc cgtccgatcg acgacgtggt
     1381 ccgcgagatc gtcaagcagg tccgcgcccg caccggggcg tcgcggtgat cttctggcgg
     1441 gtggtccgca ccgtcgtcgt cgggttctgc gcgacgatct tccgggttcg cctgatcgga
     1501 cgtgaccacg tcccggactc cggggcgtac gtcatcgccc cgtcgcaccg gtcgatcatc
     1561 gacgtgccgt tcaccgcgtt cgtgacgcgc cgccggatcc ggtttctcgc caagcaggag
     1621 ctgttctcgt cgcggatcgg ttcggcgctg ttcccgcggc tcggtggcat ccccgtcgag
     1681 cgcggcacca ccgaccgggc ggcgttgcgg gcctgtgacg cggtgctggc tgaaggtgat
     1741 ccgctggcga tcttcccgga ggggacgcga cgccacggtc ccgagctggg ggacctgttc
     1801 catggggtcg cgtacctggc gttgaaacga cgtgtaccga tcgtgccggt cgggatcggc
     1861 ggcagcgagg agatcctgcc gcgcgggcac gtgcggcccc gaccgcaccg ggttgtggtc
     1921 gtggtggggg agccgatcgt ggccgacccg tccgcgtcgg cgcgtcggcg ttccgacgtc
     1981 gcggcgctga ccgcgacgct cgcgacccgg cttcaagcct gcctcgacga tgcccgccgg
     2041 cgggccggga
//
LOCUS       DATCCH010000212         2768 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_479683, whole genome shotgun sequence.
ACCESSION   DATCCH010000212 DATCCH010000000
VERSION     DATCCH010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479683"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..421
                     /locus_tag="VIJ44_04160"
     CDS             212..421
                     /locus_tag="VIJ44_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEY5165127.1"
                     /translation="MRVLLRNPRREFDVDGPITVAALLQRLEVNPESVLVIAGDELVA
                     RDARLDDNTTVEIRPVISGGEGPRP"
     gene            418..1332
                     /locus_tag="VIJ44_04165"
     CDS             418..1332
                     /locus_tag="VIJ44_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5165128.1"
                     /translation="MKCRVCRGPAVIDVRRHNAAFCPDHFVTHCREQVRRAITDHDMI
                     GPNDRIVVAVSGGKDSLALWDLLVDLGYQPDGVYLGLGIGEYSNDSLDHARAYATDRG
                     VTLHEVDLAAEQGFTIPDAAAATHRAPCGACGLSKRHLLNRFALEHHYDTVTTGHNLD
                     DEAAVLLGNVLRWETEYLARQHPVLPATTGFARRIKPLVRLGERETAAYCVIRGIDYQ
                     VDECPMAAGNRHLHYKDVLNRLEQRSPGSKAAFFHGFLDHAHDRFADHPDRDDTGAHL
                     GCCTECGAPTTTDICAFCRLRHRATSRP"
     gene            1329..2111
                     /locus_tag="VIJ44_04170"
     CDS             1329..2111
                     /locus_tag="VIJ44_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HEY5165129.1"
                     /translation="MTGAFAAGDRVLLVDTKRRRHLITLQPGGEFHSHTGVLRHDDLI
                     GHDEGATVRTTLGARLIAVRPTLAEYVLEMPRGAQVIYPKDLGPILMLADIYPGARIL
                     ESGIGSGALTMTLLRAVGPSGHVTGYELRDDFADRARRNVEGLLGPDIPLDVEVRDTY
                     DGIDDDHLDRIVLDLPEPWRVVKHAVATLRPGGILLAYLPTIGQVARLREELADSPFG
                     MAQSLEVLQRAWHVDGQSVRPDHRMVAHTGFLTHARLLGPTV"
     gene            2108..>2768
                     /locus_tag="VIJ44_04175"
     CDS             2108..>2768
                     /locus_tag="VIJ44_04175"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEY5165130.1"
                     /translation="MNILDVILIVVIAIAGYTGYRFGLAARALSWAGLAVGLIVGVEF
                     VDDIARALQGSTPRTRLLASLAFLLLVALIGQSLGIALGSLARRHLPAAGGIRVADRV
                     AGGITGVLGVLVVVWLLIPGLANAPGWTARAVRGSAIVRQIDRVAPKPPSSLSTLGRL
                     VGDAPFPEVFRQLTSSDVGSPPSQGLPAALSNRISLAVVKVEGQACDTIQQGSGFVAG
                     PG"
BASE COUNT          406 a         1187 c          770 g          405 t
ORIGIN      
        1 ggcctgcgcc gcggcttcct cgaagggtcc agcacctggc tcgtggtcgg tgtcgccgcc
       61 accgagctcc gcgccgcgca acgcatcctc accgaaccgc cggtcaccgc caccttcgac
      121 ctcaagccgg gcgaggcggt cgagatccgc gccgtgccgc caccttcacg ctgaccaacc
      181 ggcgggaccc gccggtaccc ttggacgctc gatgagagtc ctgctccgca acccgcgccg
      241 cgagttcgac gtcgacggac ccatcaccgt cgccgcgctc ctccaacgac tcgaggtcaa
      301 ccccgaatcg gtgctcgtca tcgccggtga cgagctcgtc gcgcgcgacg cccgcctcga
      361 tgacaacacc accgtcgaga tccgtcccgt catctccggc ggtgaagggc cccgcccatg
      421 aaatgccgcg tgtgtcgcgg tcccgccgtc atcgacgtcc gccgccacaa cgccgcgttc
      481 tgtcccgacc acttcgtcac ccactgccgc gagcaggtcc ggcgagccat caccgaccac
      541 gacatgatcg gcccgaacga ccgcatcgtc gtcgccgtct ccggcggcaa agactccctc
      601 gccctctggg acctcctcgt cgacctcggc taccagcccg acggcgtcta cctcggcctc
      661 ggcatcggcg agtactccaa cgactccctc gaccacgccc gtgcctacgc caccgaccgg
      721 ggcgtcaccc tccacgaggt cgacctcgcc gccgaacaag ggttcaccat ccctgacgcc
      781 gccgccgcca cccaccgcgc gccctgcggc gcctgcggtc tctccaagcg ccacctcctg
      841 aaccgcttcg cgctcgagca ccactacgac accgtcacca ccggccacaa cctcgacgac
      901 gaagccgcgg tgctcctcgg caacgtgctc cgctgggaga ccgagtacct ggcccgccaa
      961 cacccggtcc tgcccgccac caccggcttc gcgcgtcgca tcaaacccct cgtccggctc
     1021 ggcgagcgcg aaaccgccgc gtactgcgtc atccgcggca tcgactacca ggtcgacgaa
     1081 tgccccatgg ccgcagggaa ccgccacctc cactacaagg acgtgctcaa ccgcctcgag
     1141 caacgctcac ccggctccaa agccgcgttc ttccacggct tcctcgacca cgcccacgac
     1201 cgcttcgccg accaccccga ccgcgacgac accggagcgc acctcggctg ctgcaccgag
     1261 tgcggcgccc cgaccaccac cgacatctgc gccttctgtc gcctccgaca ccgcgccacc
     1321 agccgaccat gaccggcgcc ttcgccgccg gcgaccgggt cctcctcgtc gacaccaaac
     1381 gacgccgcca cctcatcacc ctccagcccg gcggcgagtt tcactcccac accggagtgc
     1441 tccgccacga cgacctcatt ggtcacgacg agggcgcaac tgtccgcacc accctcggcg
     1501 cccgcctcat cgcggtgcga cccaccctcg ccgagtacgt cctcgagatg ccccgcggcg
     1561 cgcaagtcat ctacccgaag gatctcggcc ccatcttgat gctggccgac atctaccccg
     1621 gcgcccgcat cctcgaatcc gggatcggct ccggcgcgct caccatgacc ctcctacgcg
     1681 ccgtcggacc gtccggtcac gtcaccggct acgagttgcg cgacgacttc gccgaccggg
     1741 ctcgccgcaa cgtcgaagga ctcctcgggc cggacatccc cctcgacgtc gaggtccgag
     1801 acacctacga cggcatcgac gacgaccacc tcgaccgcat tgtcttggac ctccccgagc
     1861 cgtggcgcgt cgtgaaacac gcggtcgcta ccctccggcc gggcgggatc ctcctcgcct
     1921 accttccgac catcggccag gtcgcccgcc tccgagagga gctcgccgac tcgccgttcg
     1981 ggatggccca atccctcgag gtcctccaac gcgcctggca cgtcgacggg cagtccgtgc
     2041 gccccgacca tcgcatggtt gcgcacaccg ggttcctgac ccacgcccgg ctgctcggac
     2101 ccaccgtttg aacatcctcg acgtcatcct catcgtcgtc atcgcgatcg ccggctacac
     2161 cggctaccgc ttcggactcg cggcccgcgc cctgtcatgg gccggtctcg ccgtcggcct
     2221 catcgtcggc gtcgagtttg tcgacgacat cgcccgcgcg ttgcagggct ccacgccccg
     2281 cacccggctc ctcgcgtcgc tcgcattcct gctcctcgtg gccctcatcg gccagagcct
     2341 cggcatcgcg ctcggcagcc tggcgcgccg tcaccttccc gccgccggag gcatccgcgt
     2401 cgccgaccgc gtcgccggtg gcatcaccgg cgtgctcgga gtcctcgtcg tcgtctggct
     2461 cctgatcccc gggctcgcca acgcgcccgg ctggaccgcc cgggccgtgc gcggctccgc
     2521 gatcgtccgc cagatcgacc gcgtcgcccc gaagccgccc tcgtcgctct ccaccctggg
     2581 ccgactcgtc ggcgacgccc cgttcccgga ggtgttccgc cagctcacct cgtccgacgt
     2641 cgggtcgccc ccatcgcaag gactcccagc cgcgctcagc aaccggatct cgctcgcggt
     2701 cgtgaaagtc gaaggccagg cttgtgacac gatccagcag gggagcgggt tcgtcgccgg
     2761 cccggggc
//
LOCUS       DATCCH010000213         3109 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_312724, whole genome shotgun sequence.
ACCESSION   DATCCH010000213 DATCCH010000000
VERSION     DATCCH010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312724"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..1000)
                     /locus_tag="VIJ44_04180"
     CDS             complement(5..1000)
                     /locus_tag="VIJ44_04180"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY5165131.1"
                     /translation="MPRLVLNYDLRHPDSFGASAGATYATALDQIGWADEHGFAFVAF
                     GEHHQSPDGYLSCPLLFAAAIGGRTKTIRVRVSALLAPLYDPIRLAEEIAVADLCLGG
                     RLDVGIAAGYVREDFAMFGAEYTQRGRELESTVALLRRAWTGEPFLHRGTTVRVTPRP
                     AQDPMPLYLGGAARPAIERAARIGDGYFPPTPGRWAVYRDACVGLGKPDPGPLPPQSP
                     IFCWVTRDDKEEVWARLAPHVRHQTQSYAAWTSSGLGRPAGPFVDAERSDDVRPGGAY
                     LVLTPEETVELARDLGDDGRLNFSPLLAGIDPAWSWSMLGLVEAEVLPYLGLSPR"
     gene            complement(1152..2018)
                     /locus_tag="VIJ44_04185"
     CDS             complement(1152..2018)
                     /locus_tag="VIJ44_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004019466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY5165132.1"
                     /translation="MESQLVRFEGFEGIHLVGDRWGRDDAWPVLLMHGGGQTRHAWGN
                     TASVLADHGWNAVSLDLRGHGDSEWALNGDYSFTAYAADCVAVADQLGRPPVLVGASL
                     GGVAAMLAEGGSDRVVSAGLIVVDITHRSNPEGIDRIRDFMASGLGGFATLEDAADAI
                     AAYTPNRPKRVNPAGLMKVLRQRRDRRWYWHWDPKFLEHGRTEVPREDFQALFEAALR
                     GIRVPTLLVRGLLSDVVTEEGVQDFLDKIPGSKLADVGEAAHMVAGDQNDLFSDAVVN
                     FLEHDVRPLLPR"
     gene            2113..2769
                     /locus_tag="VIJ44_04190"
     CDS             2113..2769
                     /locus_tag="VIJ44_04190"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEY5165133.1"
                     /translation="MEQVKFHFDPLCPWCYQTSRWARRLEELGEVELEWGVFSLEIVN
                     LEEGKDRRELEARSAPALRTAITIRDALGAKAIGPFYAALGRRIWEAPPPPDDAVAAV
                     RDALGEAGLDPGLCDKALADETSWEAALAEHDALVEHTGSFGVPTMVLDAGTGPAIFG
                     PVISVLPSDEDAVTLWRHTVGLMRYGNFSELKRSRALPPDLPTATWFREQREQKKARS
                     "
     gene            complement(2805..>3109)
                     /locus_tag="VIJ44_04195"
     CDS             complement(2805..>3109)
                     /locus_tag="VIJ44_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165134.1"
                     /translation="TRDFGRAKAGVGIYVTLAQPATVSTVTVYTVEPGWNAQIYAANT
                     PSTNLAGWGKPLATGTNLGTHVQFTLRPATQARVVLLWLTFLPEGGKLDVAEIQVR"
BASE COUNT          501 a         1029 c         1113 g          466 t
ORIGIN      
        1 gcggtcagcg cggtgacagg ccgaggtagg ggagcacctc ggcttcgacg agaccgagca
       61 tcgaccatga ccaagccggg tcgatgccgg ccaagagggg actgaagttc agccggccgt
      121 cgtcgccgag gtcgcgcgcc agctcgaccg tctcctccgg ggtgagcacg agatacgcgc
      181 ctccgggtcg cacgtcgtcg gaccgctcgg cgtcgacgaa cggaccggca gggcgaccca
      241 ggccgctcga ggtccacgcg gcgtagctct gggtctgatg acggacgtgc ggcgcgagtc
      301 gggcccacac ctcttccttg tcgtcacggg tgacccagca aaagatcggg ctctgaggcg
      361 gcagcgggcc cgggtcgggc ttcccgagcc cgacacaggc gtcgcggtag accgcccatc
      421 ggccaggcgt cggagggaag tacccgtcgc cgattcgtgc cgcccgctcg atcgcgggcc
      481 gggccgcacc accgaggtac agcggcatcg ggtcttgggc cggtcgcggc gtgacgcgca
      541 ccgtcgtccc tcgatgtagg aacggctcgc cggtccacgc tcgacggagg agcgcgacgg
      601 tgctctcgag ctctcgaccg cgttgcgtgt attcggcgcc gaacatggcg aagtcttcgc
      661 gcacgtaccc ggcggcgatc ccgacatcga gccggccgcc caggcagagg tccgcgaccg
      721 cgatctcctc ggccaaccgg atcgggtcgt agagcggagc gaggagcgcc gacacccgca
      781 cccggatggt cttggtgcgg ccgccgatcg ccgcggcgaa caggagcggg cacgacaggt
      841 atccgtcggg ggactggtgg tgctcaccga acgcgacgaa agcgaacccg tgctcgtcag
      901 cccacccgat ctggtccagt gccgtggcgt aggtcgcgcc agccgacgcg ccaaacgagt
      961 cgggatggcg caggtcgtag ttgaggacca gtcggggcac ggatcggcga gtgtagggag
     1021 cacgtcgagt gggcgtcccg acctgatgct cggtcgaccc tcaggaccgt tttcgatcga
     1081 atccgattcg ctgtctggga cccgaggccg aggcagtcga cgcgggctgg cctcccgcgc
     1141 gcgccggcca gtcagcgcgg gagcagggga cggacgtcgt gttccaagaa gttgacgacc
     1201 gcatcggaga agaggtcgtt ctggtcacca gcgaccatgt gggcggcctc gccgacgtcg
     1261 gcgagcttcg agccgggaat cttgtcaagg aagtcctgga cgccctcttc agtcacgacg
     1321 tcgctgagca atcctcgaac cagcagggtc gggacgcgga tgccccgcaa cgccgcttcg
     1381 aagagggctt ggaagtcctc gcgcggcact tcggtgcgtc cgtgctccag gaacttcgga
     1441 tcccagtgcc agtaccaccg tcggtcgcgg cgttggcgaa gcaccttcat gagcccagcc
     1501 gggttcacac gcttcggccg attgggcgtg taggccgcga tggcgtcggc cgcgtcctcg
     1561 agggtggcga aaccaccaag acccgaagcc atgaagtcgc ggatccggtc gatgccctcg
     1621 ggattgctgc ggtgggtgat atccacgacg atgagcccgg ctgagaccac tcggtcgctc
     1681 ccgccttcgg ccagcatcgc cgcgacaccg ccaagcgagg ctcccacgag caccggtggg
     1741 cgccccagct gatccgcgac cgcgacacag tccgcggcgt aggccgtgaa ggaatagtcg
     1801 ccgttgagcg cccattcgct gtcaccgtgt ccgcgcagat ccagcgacac cgcgttccaa
     1861 ccatggtcgg cgagcacgct cgcggtattg ccccaggcat gacgggtctg accgccaccg
     1921 tgcatcaaga gcacgggcca cgcgtcgtcg cggccccagc ggtccccaac gagatggatg
     1981 ccctcgaatc cctcaaagcg cacgagttgc gactccacgg cggcatcctc gcatgcccga
     2041 gcaccggccg tctgggtcac cgaacgtcgg gagtacggtg gcccgaccgg caggccgagt
     2101 ccggaggtcc cgttggaaca ggtcaagttt cacttcgatc cgctgtgccc gtggtgctac
     2161 cagacgtcgc gctgggcgcg acggctcgag gagctcggtg aagtcgagtt ggagtgggga
     2221 gtgttctcgc tcgagatcgt gaacctcgag gagggcaagg atcgtcgcga gctcgaggcg
     2281 cgctccgcgc ccgcgttgcg tacggcgatc acgatccggg acgccctcgg cgcgaaggcg
     2341 atcggtccgt tctacgccgc gttggggcgc cggatctggg aggcgccgcc accgcccgac
     2401 gatgcggtcg ccgcggtgcg cgacgcactc ggcgaggcgg gcctcgaccc tggtctgtgc
     2461 gacaaggcgc tcgccgacga gacctcgtgg gaggccgcgc tggccgagca tgacgctctc
     2521 gttgagcaca ccggcagttt cggtgttccc acgatggtcc tcgacgccgg aaccgggccg
     2581 gcgatcttcg gaccggtgat cagcgtgctt ccgagcgacg aggacgcggt gacgctctgg
     2641 cgccacaccg tgggactgat gcgctacggg aacttctcgg agctgaagcg ctctcgcgcc
     2701 ctgcccccgg acctgccgac ggcgacgtgg tttcgagagc agcgcgagca aaagaaggcc
     2761 cgctcctaaa gccccaggtc gaacgcgtcg ccgaacgggt ggggtcagcg cacctggatc
     2821 tcggcaacgt cgagcttgcc gccttccggg aggaacgtga gccagagcaa gaccactcgg
     2881 gcctgcgtcg cgggacgaag ggtgaactgg acgtgggtcc caaggttggt gccggtcgcg
     2941 aggggctttc cccagcccgc caggttggtg gacggcgtgt tcgcggcgta gatctgtgcg
     3001 ttccagcctg gttcgacggt gtagacggtg acggtcgaga cggtggccgg ctgggccagg
     3061 gtgacgtaga tcccgacgcc ggccttggcg cgtccaaagt cgcgggtcg
//
LOCUS       DATCCH010000214         2571 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_11317, whole genome shotgun sequence.
ACCESSION   DATCCH010000214 DATCCH010000000
VERSION     DATCCH010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11317"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..374
                     /locus_tag="VIJ44_04200"
     CDS             153..374
                     /locus_tag="VIJ44_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018224969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3039 domain-containing protein"
                     /protein_id="HEY5165135.1"
                     /translation="MPAPVEAPSRPITDDGDHDRFAHYAKKADIARAYVTGEAIVALC
                     GKKWVPSRDPKRYPICPTCAERKAAGWRP"
     gene            453..1550
                     /gene="amrS"
                     /locus_tag="VIJ44_04205"
     CDS             453..1550
                     /gene="amrS"
                     /locus_tag="VIJ44_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007195044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HEY5165136.1"
                     /translation="MAVSSTVVPTQYWHALDDGRIQCDLCPRACKLHEGQRGVCFVRA
                     RQDDAVVLATYGRSSGFCVDPIEKKPLNHFFPGSSVLSFGTAGCNLACRFCQNWDISK
                     SKEFDTLADAADPETIAAAAERLGCRSVAFTYNDPVIFLEYAVDVADACRARGIHPVA
                     VTAGYVCDRPRRDFFAHIDAANVDLKGFTDDFYRHVCGGRLAPVLETLEYLVHDTDVW
                     VEITTLLIPGKNDADDELQAMTSWVVEHLGPEVPMHFTAFHPDFKMLDVAPTPSATLS
                     RARDIALGNGVRHAYTGNVHDPDGGCTRCSGCGTVVIERDWYQLGAYQLSDDGRCTVC
                     DAPLAGRFDGPAGTWGARRRPVRLADVRAGL"
     gene            1547..2350
                     /gene="amrB"
                     /locus_tag="VIJ44_04210"
     CDS             1547..2350
                     /gene="amrB"
                     /locus_tag="VIJ44_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018877605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HEY5165137.1"
                     /translation="MTEEARRRPPAVAGTFYPADPDVLAAQVDQLVATDTTGPTPKAL
                     IVPHAGYAYSGVVAGRGYATLGAGRDRIERVVLLGPAHHVPLVGLATTSADQWVTPLG
                     SVRIDDEARRVVLALPGVEIDDRAHATEHSLEVHLPFLQRTLQRFSLLPLVVGHADAE
                     TVATVLDTVWGGPETLIVVSTDLSHYHDHATASVLDRRTADAITAGDPEAIRPADACG
                     AHPVRGLLVAARHHGLRTRLVDLRNSGDTAGPRDRVVGYGAFALAEDAA"
     gene            2347..>2571
                     /locus_tag="VIJ44_04215"
     CDS             2347..>2571
                     /locus_tag="VIJ44_04215"
                     /inference="COORDINATES: protein motif:HMM:NF013988.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMMECR1 domain-containing protein"
                     /protein_id="HEY5165138.1"
                     /translation="MTTTVVLSDTDRRALLDVAAAVIGQAVARRDPRIPDPSDFGGRL
                     SEPGASFVTLERGDQLLGCIGSLVAVEPLVV"
BASE COUNT          370 a          953 c          860 g          388 t
ORIGIN      
        1 tcgacaccat ccgcggcgcg tgagcgggcc cacccgagca gaccgatgtc gtggatgcga
       61 cgtcaaccgg ttgggcacgc cgcccggtgg cgccggcggc ccgtccggag accggcggta
      121 cgatcgcttg cgtgacaagc gagacgacgc tcatgccggc gccggtcgag gctccttctc
      181 ggccgatcac cgacgacggc gaccacgacc ggttcgcgca ctacgccaag aaggccgaca
      241 tcgcacgggc ttacgtgacc ggcgaggcga tcgtcgcgct gtgcggcaag aagtgggtac
      301 cgagccggga cccgaagcgg tacccgatct gcccgacttg tgccgagcgc aaagccgcgg
      361 gctggcggcc ctgagccacc ctggtgtccg gccggctggt tgggcgtccg accggggaag
      421 gtcggcgccc cgttcggggt tgaatcaagg ctatggcagt ttcatccacg gtcgtcccga
      481 cccagtactg gcatgcgctc gacgacggtc gcatccagtg cgatctgtgc ccgcgagcct
      541 gcaagctcca cgagggccaa cgcggggtgt gcttcgtccg agcccgtcaa gacgacgccg
      601 tcgtgctcgc cacctacgga cgctcgagcg gcttctgcgt cgacccgatc gagaagaagc
      661 cgctcaacca cttctttccc ggcagctcgg tgctgtcgtt cgggaccgcc ggttgcaacc
      721 tggcgtgtcg cttctgtcag aactgggaca tctccaagtc caaggagttc gacaccctcg
      781 ctgacgccgc cgacccggag acgatcgccg ccgccgccga gcggctcggc tgccggtcgg
      841 tcgccttcac gtacaacgac ccggtcatct tcctcgagta cgccgtcgac gtggccgacg
      901 cgtgccgagc gcgaggcatc catcccgtcg cggtgacggc cgggtacgtc tgcgatcggc
      961 ctcgacggga ctttttcgcg cacatcgacg ctgcgaacgt ggacctcaaa ggcttcaccg
     1021 acgacttcta ccgccacgtc tgcggcggcc gcctggctcc cgtcctcgag accctcgagt
     1081 atctggtcca cgacaccgac gtctgggtcg agatcacgac cttgctcatc ccgggcaaga
     1141 acgacgccga cgacgagctg caagcgatga cgtcgtgggt cgtcgagcat ctgggacctg
     1201 aggtcccgat gcacttcacc gcgttccatc ccgacttcaa gatgctcgac gtcgccccaa
     1261 ccccgtcggc gacgctgagc cgggcacgcg acatcgcgct cggcaacggg gtccgccacg
     1321 cgtacaccgg gaatgtccac gaccccgacg ggggctgcac ccgctgcagc ggatgcggga
     1381 cggtggtgat cgaacgggac tggtatcagc tcggtgccta tcagctgagc gacgacggtc
     1441 ggtgcacggt ctgtgacgct cccctcgccg gccgcttcga cgggcccgcg gggacgtggg
     1501 gtgcgcgacg ccggccggtg cgcctggcgg acgttcgggc cgggctgtga ccgaagaggc
     1561 ccgccgccga ccgccggccg ttgccggcac cttctatccc gctgatcccg acgtcctcgc
     1621 ggcgcaggtc gaccagctgg tggcgacgga cacgaccggc ccgacgccga aagcgctcat
     1681 cgtcccccac gccgggtacg cctactccgg tgtcgtggcc gggcgcgggt acgcgacgct
     1741 cggcgcgggc cgagaccgga tcgaacgggt cgtgctgctc ggccctgcgc accacgttcc
     1801 cctggtcggg ctggccacca cctccgccga ccagtgggtg acaccgctcg gctctgttcg
     1861 catcgacgac gaggcgcgtc gcgttgtcct cgcgctcccg ggcgtcgaga tcgacgaccg
     1921 ggcgcacgcg acagagcaca gcctcgaggt gcacctcccg ttcctgcagc gcaccctcca
     1981 acggttctcg ctgctgcccc tcgtggtcgg tcacgctgac gcggagacgg tcgcgacggt
     2041 gctcgacacc gtctggggcg gtcccgagac cctcatcgtc gtgagcaccg atctcagcca
     2101 ctaccacgat cacgccaccg cgtcggtcct cgaccgccgg acggctgacg ccatcacggc
     2161 cggcgacccc gaagcgattc gtcccgccga cgcgtgcggg gcgcatccgg tccgcggtct
     2221 gctggtcgcg gcgcgccacc acggcctgcg gacccggctc gtcgatctcc gcaactccgg
     2281 cgacaccgcc ggcccccgtg accgcgtcgt gggatacggg gcgttcgccc tcgccgagga
     2341 cgcggcgtga ccaccaccgt cgtgctgagt gacaccgacc ggcgggcgct cctcgacgtg
     2401 gccgccgccg tgatcgggca agccgtcgcg cgccgcgacc cccggatacc cgacccctct
     2461 gatttcggcg gacggctcag cgagcccggc gcgtcgttcg tcaccctcga gcggggcgat
     2521 cagctgctgg gctgcatcgg cagcctcgtg gccgtcgagc cgctcgtcgt c
//
LOCUS       DATCCH010000215         2724 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_521385, whole genome shotgun sequence.
ACCESSION   DATCCH010000215 DATCCH010000000
VERSION     DATCCH010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521385"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="VIJ44_04220"
     CDS             complement(<1..770)
                     /locus_tag="VIJ44_04220"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5165139.1"
                     /translation="MRNGRMAIVTGASSGIGRAVALGLADLGWSVALGARRTERLEDA
                     AVDARERGGQAFVHSLDVTDPESLAVFFAAAEAALGPVGVLVNNAGVATPGWLDRVPV
                     AALQREVATNLLGPILMSRLAIGSWRERGQGGDLVFVTSDATRHPRPRMAGYTATKAG
                     LEAFSQALAMELEGSGVRSTTIRVGPTLTDFGFGWPTDDLEDLMGYWPRFGLQRHAGV
                     LDPSAVAHAVVTAVTAPAGVHFDTIEVQPEAPVGDDGPA"
     gene            complement(778..1704)
                     /locus_tag="VIJ44_04225"
     CDS             complement(778..1704)
                     /locus_tag="VIJ44_04225"
                     /inference="COORDINATES: protein motif:HMM:NF012431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165140.1"
                     /translation="MAADIPERAGRVRVLVGPGVVDAGMVDALRAFAAAGDLGVANTW
                     GAKGVFAWDSPHHLGTCGLQARDFELLGFADADLVVGVGLDEDESPHDRLALAPVVEL
                     DPGRLWEAVGRVRATGVPLTNALYPRLAAVAQPGFIDDKVPLHPARAVADLGAVLPTN
                     GLLSADPGLAGLWVARTFPTPALASGAPRRVVVPARRGPAVGLRLAVDAARAGRPSIV
                     VTTAPLDAATAAVLDQVVDERLPVVMAVWDDTASDFGRVEDHQAHLAAALAAARPTVV
                     EVPVSLGDTRQLIAAAGEVVAWGGLTSAEARG"
     gene            1808..2512
                     /locus_tag="VIJ44_04230"
     CDS             1808..2512
                     /locus_tag="VIJ44_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165141.1"
                     /translation="MSSRNVYLHEYIDIVGEGAMPYMEHTAERGASHASGGTLDLVGT
                     WYTMGSTGRWPQVINLWECPHGWDSWRALMERTNLRRTKTPELEDWWRRALEVRTGGV
                     DRLMAGAPGCPSLAELQDDGVQASVFVHELSRCRAGAALDYLAAVREEWQPVLGEYGH
                     RLVGLYEVLLTDREVLTIWATDPESHCSLQRAATDATDERIARWRAHAREFLTDWREE
                     LMTPHPKTALSAGDSL"
     gene            2509..>2724
                     /locus_tag="VIJ44_04235"
     CDS             2509..>2724
                     /locus_tag="VIJ44_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020549585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY5165142.1"
                     /translation="MSAPDRGLLTGVRIIESSLLGPGELTSFLADLGADVIKVEPPQG
                     DYIRRMTWPIVEGDSLLHLHIHRGKRSI"
BASE COUNT          426 a         1002 c          949 g          347 t
ORIGIN      
        1 gccgggccgt catcaccgac gggagcctcg ggttgcacct cgatggtgtc gaagtggacg
       61 cccgccgggg ccgtgacggc ggtgaccacc gcgtgcgcca ccgcgctcgg gtcgagcacg
      121 ccggcgtggc gttggaggcc gaaccggggc cagtagccca tcagatcctc gaggtcgtcg
      181 gtcggccagc caaacccgaa gtcggtgagc gtcggaccga cccggatggt cgtcgaacgg
      241 acaccggacc cctcgagctc catggcgagc gcctgcgaga acgcctcgag gccggccttc
      301 gtcgccgtgt accccgccat gcgcggtcgg ggatgacgcg tcgcgtccga cgtcacgaac
      361 accagatcgc cgccctggcc ccgctcgcgc cacgaaccga tcgccaggcg gctcatgagg
      421 atcggaccga gcaagttggt ggcgacctcg cgctggagcg cggcgaccgg gacgcgatcg
      481 agccagcccg gcgtcgcgac acccgcgttg ttgacgagga cgcccaccgg acccagcgct
      541 gcttcggcgg cagcgaagaa caccgcgagg gactcggggt ccgtcacgtc gagggaatgg
      601 acgaaggcct gaccgccgcg ctcgcgggcg tcgacggcgg cgtcctcgag ccgctcggtg
      661 cggcgcgcgc cgagcgcgac gctccagccc aggtcggcga gcccgagcgc cacggcccgg
      721 ccgatccccg atgacgctcc ggtcacgatg gccattcgtc cgttccgcac ggagctgtca
      781 ccccctcgcc tcggcggagg ttaggccgcc ccatgcgaca acctctcccg cggcggcgat
      841 gagctggcga gtgtcgccca ggctgacggg tacctcgacg accgtgggcc gcgccgccgc
      901 gagcgccgcc gcgaggtgcg cctgatggtc ctcgacgcga ccgaagtccg aggcagtgtc
      961 atcccagacc gccatgacga ccgggaggcg ttcgtcgacg acctggtcga ggaccgcggc
     1021 ggtcgcggcg tcgagcggcg ccgtggtcac cacgatcgag ggacgaccgg cccgggccgc
     1081 gtcgaccgcg agtcgcagcc cgaccgcggg gccgcgacgg gcggggacga ccacgcgccg
     1141 gggagcaccc gacgccagcg ctggggtcgg gaacgtccgg gccacccaca gcccggccag
     1201 ccccgggtcg gcgctgagga gcccattcgt cgggagcaca gcccccaggt ccgcgacggc
     1261 ccgcgccggg tgcagcggaa ccttgtcgtc gatgaacccg ggttgcgcga ccgcggccag
     1321 acggggatac agcgcattcg tcaacgggac cccggtcgcc cgcacacgac cgacggcctc
     1381 ccacagccga ccggggtcca gctccacgac tggcgccaag gccaaccggt cgtggggtga
     1441 ctcgtcctca tcgagcccga cgcccacgac gagatcggcg tcggcgaagc ccaggagctc
     1501 gaagtcgcgg gcctgcagcc cgcacgtgcc tagatgatgc gggctgtccc acgcgaagac
     1561 gcccttcgcg ccccaggtgt tggcgacgcc aagatcgccc gccgccgcga acgcgcgcag
     1621 ggcatcgacc atcccggcgt cgacgacgcc gggaccgacc agcacccgca cccgaccggc
     1681 ccgctccgga atgtcggctg ccatgaccgc atcgtggcat gcgaacgacc gacccaactg
     1741 gcgatccgcg gcgctggtcg acggtgctcg ggatccgaaa cgaggcggtc cgatacgatc
     1801 ccgcccggtg tcgagtcgga acgtgtatct ccacgagtac atcgacatcg tgggggaagg
     1861 cgcgatgccg tacatggagc acaccgcgga gcgcggggcg tcccacgcgt cggggggaac
     1921 cctggacctg gtcggcacct ggtacacgat gggttcgacc ggccgttggc cccaggtcat
     1981 caacctctgg gagtgccccc acgggtggga cagctggcgc gcgctcatgg agcgcaccaa
     2041 cctccggcga acgaagacac ctgagctcga ggactggtgg cggcgggccc tcgaggtccg
     2101 caccggaggt gtcgaccggc tcatggccgg tgcgcccgga tgcccatccc tcgccgagct
     2161 gcaagacgac ggcgtgcagg ccagcgtgtt cgtccacgag ctgtcccgct gccgggctgg
     2221 cgccgccctc gactacctcg cggccgttcg tgaggaatgg cagccggtgc tgggggagta
     2281 cgggcaccgc ctcgtcggcc tttacgaggt gctgctcacc gatcgtgaag tcctcaccat
     2341 ctgggcgacc gaccccgaat cgcattgctc gctgcaacgc gccgcgaccg acgccaccga
     2401 tgaacggatt gctcgttggc gggcccacgc tcgggagttc ctcaccgact ggcgcgaaga
     2461 gctcatgaca ccgcatccca agaccgcgct ctccgccggc gattcgctgt gagcgcgccc
     2521 gaccgtggcc tgctcacggg cgtgcggatc atcgagagct cactcctcgg tcccggtgag
     2581 ctcacgtcct ttctcgccga tctcggcgct gacgtcatca aggtcgagcc gccccaaggc
     2641 gactacatcc gccgcatgac ctggccgatc gtcgaaggcg actcgttgct gcacctgcac
     2701 atccaccgcg ggaagcgcag catc
//
LOCUS       DATCCH010000216         2436 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_218877, whole genome shotgun sequence.
ACCESSION   DATCCH010000216 DATCCH010000000
VERSION     DATCCH010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218877"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VIJ44_04240"
     CDS             <1..293
                     /locus_tag="VIJ44_04240"
                     /inference="COORDINATES: protein motif:HMM:NF025204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4193 family protein"
                     /protein_id="HEY5165143.1"
                     /translation="ADGEEEEDVLELDEELHPDDVEAPLDALLQERTASATLEDEEEE
                     VEEEEADTDERGEGPTKIVPRRPGEFLCASCFLVLPRNQLADEERMLCRDCA"
     gene            359..1846
                     /gene="lnt"
                     /locus_tag="VIJ44_04245"
     CDS             359..1846
                     /gene="lnt"
                     /locus_tag="VIJ44_04245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEY5165144.1"
                     /translation="MLLALAYPPADLGPLALVALVPLLWAWRDATPLGAARDGFVFAL
                     AFLAILMAGLTNTGYTGTVSLIVAAGFYYAATGALVAAFARRGIGAPWLTAAVWVFFE
                     GLRGRWPLGGLAWGEIGIALHNVGPARALASFGGVALVTFVVVATNGFIVDLLVAVHR
                     RTPRPLTLTAAGLVALLAVSVVADLTRYQPRDTGHLRFALLQGDRQEGPSSAQQIADQ
                     DLTNAHFALADQLHGHYDLIVFPESALDYDPEEFPPLRQRIVDLAAKHDAVVLVNARY
                     RAPNGLLYNANLAYGPDGRLLGVYAKQHLVPFGEYVPLRDQLTFISDLRQIPYDFTAG
                     NRRVLFKAGGRPFGTVICYESAYAPLVRDFVRDGAQALVVSTSDRSYGRSGIAATHVA
                     TGQMRAAETGRPVLHAALSGVTAVIDADGRVRQTTSLFTSALTTGTVTTTTGETPFVR
                     FGEWVLVGSALVLLGAAFAASRRERRGVPVDSPSHADRAEGAPGA"
     gene            1839..2414
                     /locus_tag="VIJ44_04250"
     CDS             1839..2414
                     /locus_tag="VIJ44_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165145.1"
                     /translation="MPDDHDSADKAMEFLVYGPIGFALYLRDTAPSFLKLFVARGRSV
                     LDEQRKSVGDQLGQARSVGEFATNYGGPQVSRIVTEGLTRVRERTEEAIDALNTLIGN
                     PTATPPPAAGGPGPDSAPPPPPEPTAPAATANGRHLAIPDYDELSASQVVEHLDGLSR
                     TELDAIREYETEHRARNTVLGKIEQLNRPVA"
BASE COUNT          352 a          925 c          796 g          363 t
ORIGIN      
        1 tcgcggacgg cgaagaggaa gaggatgtcc tcgaactcga cgaggagctg caccccgacg
       61 acgtcgaagc gcctctcgac gcgctcctcc aggaacgcac cgcgagtgcc accctcgagg
      121 acgaagagga agaggtcgaa gaggaagagg ccgacaccga cgagcgtggc gaaggaccca
      181 ccaagatcgt gccccgccgc ccgggagagt tcctttgcgc gtcgtgcttc ctcgtgctgc
      241 cccgcaacca gctggccgac gaggagcgaa tgctctgccg cgactgcgcc tgaacgcctc
      301 agcgccgtcg ccgcggcgtc gcacccagac gcggctcctg gccgcggtgg tgtccggggt
      361 gctgctcgcc ctcgcgtacc cgccggccga cctcggcccc ctggccctcg tcgcgcttgt
      421 cccgctgctg tgggcttggc gcgacgccac cccgctcggc gcggcccgcg acgggtttgt
      481 attcgccctc gcgttcctcg cgatcctcat ggccgggctc accaacaccg gctacacggg
      541 caccgtctcg ctcatcgtcg ccgcgggctt ctactacgcg gcgacgggag cactcgttgc
      601 cgcgttcgcc cgccgtggca tcggtgcccc atggctgacc gcggcagtgt gggtgttctt
      661 cgaagggctg cgcggacgat ggcccctcgg cggcctggcc tggggcgaga tcggcatcgc
      721 gctccacaac gtcgggccgg cccgggcgct cgccagcttc ggtggggtcg ccctggtgac
      781 cttcgtggtc gtggcgacca acgggttcat cgtcgacctc ctcgtcgcgg tgcaccgccg
      841 tacgccgcgt cctctcaccc tcaccgcggc cgggctggtg gcgctgctgg cggtgtcggt
      901 cgtcgccgac ctcacccgct atcagccccg cgacaccggt cacctccgct tcgcgttgct
      961 ccaaggcgac cgtcaggaag ggccgagcag cgcccagcag atcgccgatc aggatctcac
     1021 caacgcgcac ttcgcgctcg ccgaccagct gcacggccac tacgacctga tcgtgttccc
     1081 cgaatctgcg ctcgactacg accccgagga gttcccgccg ctgcggcaac ggatcgtcga
     1141 cctcgcggcc aagcacgacg cggtcgtgct cgtcaacgcc cgctaccgcg ccccgaacgg
     1201 cctgctctac aacgccaacc tcgcctacgg gcccgacggg cggcttctcg gcgtctacgc
     1261 caagcagcac ctcgtaccgt tcggtgagta cgtcccgctg cgcgaccagc tgacgttcat
     1321 cagcgacttg cgccagatcc cgtacgactt caccgccggg aaccgccgcg tcctgttcaa
     1381 agccggcggg cgaccgttcg gcacggtcat ctgctacgag tcggcgtacg cgccgctggt
     1441 acgtgacttc gtccgtgacg gcgcgcaggc gctggtggtg agcaccagcg accggtccta
     1501 cggtcgctcc gggatcgccg cgacgcatgt cgcgacgggc cagatgcggg ccgcggagac
     1561 cggccgtccg gtgctgcacg ctgcgctgtc cggtgtcacc gcggtcatcg acgctgacgg
     1621 tcgggtgcga cagaccacct cgttgttcac gagcgccttg acgaccggca cggtcaccac
     1681 caccaccggg gagaccccgt tcgtccggtt cggtgaatgg gtccttgtcg gctcggcgct
     1741 ggtgttgctc ggcgcggcgt tcgccgccag ccggcgggaa cggcgcggcg tccccgtaga
     1801 ctcgccctca cacgccgacc gagctgaggg ggcacccggt gcctgacgac cacgacagtg
     1861 ccgacaaggc catggagttc cttgtctatg ggccgatcgg cttcgcgctg tacctgcgcg
     1921 acaccgcgcc gtcgttcctc aagctcttcg tcgcccgggg ccgctcggtg ctcgacgagc
     1981 agcgcaagtc ggtcggggac cagctcggcc aggctcgctc ggtgggggag ttcgcgacca
     2041 actacggagg accccaggtc agccggatcg tcaccgaagg cctgacccgg gtgcgggaac
     2101 ggaccgagga ggccatcgac gcgctgaaca ccctgatcgg caacccgacc gccacgccgc
     2161 ccccggcggc tgggggacca ggcccagact cggctccacc tccgcccccg gaacccacgg
     2221 cgccggccgc gaccgccaac ggacggcatc tcgcgatccc cgactacgac gagctatctg
     2281 cctcccaggt cgtcgagcac ctcgatgggc tctcccgcac cgagctcgac gccatccgcg
     2341 agtacgagac cgagcaccgg gcccgcaaca ccgtgctcgg caagatcgag cagctcaacc
     2401 gcccggtcgc ctgagcctcg ccggtggagc actgcc
//
LOCUS       DATCCH010000217         2503 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_136254, whole genome shotgun sequence.
ACCESSION   DATCCH010000217 DATCCH010000000
VERSION     DATCCH010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136254"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="VIJ44_04255"
     CDS             <1..644
                     /locus_tag="VIJ44_04255"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY5165146.1"
                     /translation="TRLAGPARRTPSLVVDLSTLWAGPLCARLVGDATGLRVVKVEDP
                     QRPDGARAGPPAFWHRLNATKDHTAVEFTTEAGRRDLAALLDDADVVITSARPRALDA
                     LGLHPTEHAARGAVWVTITGYGLTGPWRNRVAFGDDAAVAGGAAVAAGSADHPAFVLD
                     AVADPLSGLCGAAATLDALATRRGALIDVSLRDVVADALTAGQYAAHTDQEVA"
     gene            644..1135
                     /locus_tag="VIJ44_04260"
     CDS             644..1135
                     /locus_tag="VIJ44_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY5165147.1"
                     /translation="MARLEMPEGDQPEIVRVWGLRPEMGEAVQRLSYAVYSQSALPAR
                     VREAARMRIAHINDCAVCRGWRIPELAEQGVDEELYEHVTDPDAGTYSDQERLAIEFA
                     ERFALDHRSIDDAFFARLHEHFSDAEILDLTICVGNWLAFGRLTMILDLDEACAVRPR
                     AAV"
     gene            1132..2325
                     /locus_tag="VIJ44_04265"
     CDS             1132..2325
                     /locus_tag="VIJ44_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY5165148.1"
                     /translation="MTTTTVDFFDPALFAGGPPHDLFRRLRSEQPVCFLPEPAGPGFW
                     GVFAYDDVVEVSRHPQLFGSHPNTMITDVGNDGGGAGELMLNQDPPRHTKLRKLVNRG
                     FTPRQVALLEPRVRDLVDRLLTRAVDRRNLDLVADIAVELPLQVIAELVGVPEDERHT
                     VFAWTERMMSSDPESTSFGADARDAMTEMFAYAGRLAAEREQTPGDDLLSVLLAAEVD
                     GERLTQLDVDLFFMLLMNAGSETTRNLITGGMLSLFEHPEQRDRLRDAPELGPHAVEE
                     MLRWVTPVMRFRRTAREDTTLGGQQIAAGDKVVVWYTSANRDERQFPDAGSFDVTRTP
                     NDHVAFGGGGPHFCLGAGLARLEARVLFEALLERLPGLRPDGPIARSGSSFINGITAM
                     PVAVA"
     gene            complement(2297..>2503)
                     /locus_tag="VIJ44_04270"
     CDS             complement(2297..>2503)
                     /locus_tag="VIJ44_04270"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY5165149.1"
                     /translation="GDRATARWYLRETQRTVNGGQEVLGCYDDTVVRDEDGWRFARRR
                     FWVLYRGPRELAGEVFRRPPPASP"
BASE COUNT          362 a          972 c          806 g          363 t
ORIGIN      
        1 cgacgcgcct cgcgggaccg gctcggcgca ccccgagcct cgtcgtcgac ctgtcgaccc
       61 tgtgggccgg gccgctgtgc gcccggctcg ttggggacgc caccgggctc cgggtggtca
      121 aggtcgagga cccgcagcgc ccggatggcg cccgcgccgg accgccggcg ttctggcacc
      181 gcctcaacgc gacgaaggac cacaccgcgg tcgagttcac caccgaggcc gggcggcggg
      241 acctcgccgc gctgctcgac gacgccgacg tcgtcatcac gtcggcccgc ccccgtgcac
      301 tcgacgcgct cggcctccat cccaccgagc acgccgcccg cggcgcggtg tgggtgacga
      361 tcaccgggta cggactgacc ggcccgtggc gcaaccgggt ggcgttcggc gacgatgccg
      421 cggtcgcggg cggcgccgcg gttgctgccg gcagcgcgga ccacccggcg ttcgtcctcg
      481 acgccgtcgc cgacccgctc agcggcctct gcggcgcggc cgcgacgctg gacgcgctcg
      541 ccacccggcg cggcgcgctc atcgatgtct ccttgcgcga cgtcgtcgct gacgcgctca
      601 ccgccgggca gtacgctgcg cacaccgacc aggaggtcgc ataatggccc gactcgagat
      661 gcccgaaggc gaccagcccg agatcgttcg tgtctggggg ctgcgtcccg agatgggcga
      721 agcggtgcag cgcctctcct acgcggtgta ctcccagagc gccctacccg ctcgggttcg
      781 cgaggcggcc cgcatgcgaa tcgcgcacat caacgactgc gccgtctgtc gaggctggcg
      841 catccccgaa ctcgccgagc aaggcgtcga cgaggagctc tacgagcacg tcaccgaccc
      901 tgacgcgggg acgtactcgg accaggagcg actcgcgatc gagttcgccg aacggttcgc
      961 cctcgaccac cgctcgatcg atgacgcctt cttcgcccgc cttcacgagc acttcagcga
     1021 cgccgagatc ctcgacctga cgatctgcgt cgggaactgg ctggccttcg ggcgcctgac
     1081 catgatcctc gacctcgacg aggcctgcgc ggtgcgcccc cgagccgcgg tgtgacgacc
     1141 accaccgtcg acttcttcga cccggccctg ttcgccggcg gaccaccgca cgacctgttt
     1201 cgccggctcc gctccgagca gccggtctgc ttcctccccg agccggccgg tcccggcttt
     1261 tggggcgtct tcgcctacga cgacgtcgtc gaggtgtcgc gccaccccca gctcttcggc
     1321 tcgcacccca acacgatgat caccgacgtg ggcaacgacg gcgggggcgc cggggagctc
     1381 atgctgaacc aggatccgcc gcgccacacc aagctccgca agctcgtcaa ccggggcttc
     1441 actccccgtc aggtggcgct gctcgaacct cgcgtccggg atctcgtcga ccggctcctc
     1501 acgcgcgccg tcgaccgtcg aaacctcgac ctcgtcgccg acatcgcggt cgagctcccc
     1561 ttgcaggtca tcgccgagct cgttggcgtc cccgaggacg agcggcacac cgtgttcgcc
     1621 tggaccgaac gaatgatgag cagcgacccc gagagcacca gcttcggtgc cgacgcccgc
     1681 gacgcgatga ccgagatgtt cgcctacgcc ggtcgtttag ccgctgaacg cgagcagact
     1741 cccggcgacg acctgctcag cgtcctgctc gcggccgagg tcgacggcga gcgcctcacc
     1801 cagctcgatg tcgacctgtt cttcatgctg ctcatgaacg ccgggagcga gaccactcgc
     1861 aacctgatca ccggaggaat gctctccctc ttcgagcacc ccgagcagcg agaccgactc
     1921 cgcgacgccc ccgagctcgg cccccacgct gtcgaggaga tgctgcgctg ggtcacaccg
     1981 gtgatgcggt tccgccgcac cgcccgtgaa gacaccaccc tcgggggaca gcagatcgcg
     2041 gccggagaca aggtcgtcgt ctggtacacg tccgccaacc gggacgagcg ccagttcccc
     2101 gacgccgggt cgttcgacgt cacccgcacc cccaacgatc acgtcgcgtt cggtggcggc
     2161 ggcccgcatt tctgcctcgg tgccggcttg gcccgactcg aggcccgcgt cctcttcgag
     2221 gcgctgctcg aacggctccc gggactgcgg cccgacgggc ccatcgcgcg gtcggggtcc
     2281 agcttcatca acggcatcac ggcgatgccg gtggcggtcg cctgaacacc tccccggcga
     2341 gctcccgtgg cccccggtag aggacccaga accggcgacg agcgaaacgc cagccgtcct
     2401 cgtcacggac caccgtgtcg tcgtagcaac cgagaacctc ttggccccca ttgacggtgc
     2461 gttgcgtctc gcgcaggtac cagcgcgccg tcgcccgatc gcc
//
LOCUS       DATCCH010000218         2201 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_426744, whole genome shotgun sequence.
ACCESSION   DATCCH010000218 DATCCH010000000
VERSION     DATCCH010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426744"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="VIJ44_04275"
     CDS             complement(<1..551)
                     /locus_tag="VIJ44_04275"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEY5165150.1"
                     /translation="MTDVRIAGVGLHPFGRFPDRTVTDLGVAAVRAALSEAGDPSFDA
                     AFCGTAYAGVAAGHKVLGAIGRTGGPILDVEAGCASGAAALMLAAGAIDAGQYDTVLV
                     FGIEKMPKGIIRSSFFEPWREAAGLAATPAYFALRAQRLQRETGLTKEQLAGVVVKNR
                     RHGVHNPDAMFQAETTVEAVLGSR"
     gene            complement(548..952)
                     /locus_tag="VIJ44_04280"
     CDS             complement(548..952)
                     /locus_tag="VIJ44_04280"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEY5165151.1"
                     /translation="MSGRHVPVRTGLFEETDGGAVQLLGARCPSCGRYLFPRSELCPY
                     CSSTDVEAVVLSDVGELWGWTVVQTAPPGYEGPVPFGFGVVELPEGLRIITRFELSAT
                     DLDFGLPMRLSVAELHDNDDGETIVTYTFVPA"
     gene            complement(949..>2201)
                     /locus_tag="VIJ44_04285"
     CDS             complement(949..>2201)
                     /locus_tag="VIJ44_04285"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY5165152.1"
                     /translation="EGGRHGRGQARAGRVVTGPLDGIRVLDLGTRIAAPFCAGLLGEQ
                     GAEIIKVEQPHRGDFMREIGPFATTDDGDRPYSLFWAVEGRGRKSVTLDLRQQAGQDL
                     FRRLAATADVVVENFRPGTLENWGLAPSSLDTRLIVVRISSFGQDGPHARRPGLDRVG
                     IGYGGLLHLTGYPDRPPVRVGVTISDYLTGVFAAHAATCALYARDTRRDGLGAVIDAA
                     LYGAALRVLEWTLAGYDQLGVIRNREGNRLANSAPLDNYPTVDGKYVCVVAGSDANFA
                     RLCKAMERPELVDDPRFARLSDRAAHGDEINTIVADWTSGLDAKTVEERCVACDVPVA
                     TAYTAEDIFSDPHIAARGDLVTVDDPVVGPLRQQAPYPRRVGESPPVPSGAPRLGEHT
                     RTVLGETLGVSDHELDQLAVDGVI"
BASE COUNT          327 a          760 c          793 g          321 t
ORIGIN      
        1 cgcgagccga ggaccgcctc gacggtcgtc tccgcctgga acatggcatc cggattgtgg
       61 acgccgtgac gtcggttctt caccacgacg cccgcgagct gctccttggt caggccggtc
      121 tcgcgttgga ggcgctgcgc ccggagcgcg aagtaggccg gtgtcgcggc cagtcccgca
      181 gcctcgcgcc acggctcgaa gaacgacgag cggatgattc ccttcggcat cttctcgatc
      241 ccgaacacga gcacggtgtc gtactggccc gcgtcgatgg cgccagccgc gagcatgaga
      301 gccgcagctc cgctggcgca gccggcctcg acatcgagaa ttgggccccc ggtccgtccg
      361 atggctccga gcaccttgtg accggcggcg acgcccgcgt acgcagtgcc gcagaacgcg
      421 gcgtcgaagg aggggtcgcc ggcctcggac agcgcggccc gtaccgcggc gacaccgagg
      481 tcggtgacgg tccggtcggg gaaccggccg aaggggtgca gccccacccc ggcgatccgg
      541 acgtcggtca cgccggcacg aaggtgtagg tgacgatggt ctcgccgtcg tcgttgtcgt
      601 ggagctcggc gacgctgagt cgcatcggca acccgaaatc gaggtccgtg gcggacagct
      661 cgaaccgggt gatgatccgc aggccctcgg ggagctccac gacgccgaac ccgaacggaa
      721 ccggcccctc atagcccggc ggtgcggtct ggaccacggt ccagccccac agctcgccga
      781 cgtcggagag cacgaccgct tcgacgtcgg tggacgagca gtaggggcag agctcgctcc
      841 gggggaagag gtaccggccg cacgacgggc agcgggcgcc aagaagctgc acggctccgc
      901 catcggtctc ctcgaagagc ccggtccgca ccgggacgtg gcgcccgctc atatgacacc
      961 gtcgacggcg agctggtcga gctcgtggtc cgacaccccg agggtctcgc ccaggacggt
     1021 ccgggtgtgc tctccgagcc ggggggcgcc cgaaggcacc ggaggggact cgccgacccg
     1081 acgggggtag ggcgcctgtt ggcgcagcgg gccgacgacg ggatcgtcga cggtgacgag
     1141 gtccccgcga gcagcgatat gcgggtcgga gaagatgtct tcggcggtgt aggccgtcgc
     1201 gaccggcacg tcacacgcga cgcagcgctc ttcgaccgtc ttggcgtcga ggccactcgt
     1261 ccagtcggcc acgatcgtgt tgatctcgtc gccgtgcgcc gcacggtcgc tcaggcgggc
     1321 gaaccgcggg tcgtcgacga gctcgggtcg ctccatcgcc ttgcacagcc gggcaaagtt
     1381 cgcgtccgat ccggcgacga cgcatacgta cttgccgtcg acggtcggat agttgtcgag
     1441 cggagccgag ttggcgagcc ggttcccttc ccggttgcgg atgacgccga gctggtcata
     1501 gcccgccagt gtccactcga gcacccgcag ggccgctccg tagagggcag cgtcgatgac
     1561 cgcgccgagt ccgtcccgtc gggtgtcgcg ggcgtagagc gcgcacgtcg cggcgtgcgc
     1621 cgcgaagacc ccggtcagat agtccgagat cgtcaccccg acccgcaccg gtgggcggtc
     1681 gggatatccg gtcagatgca gcagccctcc gtagccgatc ccgacccggt cgaggcccgg
     1741 gcggcgcgcg tgtggtccgt cctgcccgaa gctgctgatc cgcacgacga tcagccgcgt
     1801 gtcgagggac gacggcgcga gaccccagtt ctcgagggtt ccgggccgga agttctcgac
     1861 gacgacgtcg gcggtggcgg cgagccgccg gaacaggtcc tgaccggctt gctggcgcag
     1921 gtcgagggtg acgctctttc ggccccgtcc ttccacggcc cagaagagcg agtaggggcg
     1981 gtcaccgtcg tcggtcgtcg cgaaggggcc gatctctcgc atgaagtcgc cccggtgagg
     2041 ctgttccacc ttgatgatct cggctccttg ctcgccgagg agcccggcgc agaacggcgc
     2101 cgcgattcga gttccgaggt cgaggacgcg gatcccgtcc agcgggccgg tcacgacccg
     2161 gcctgctcgc gcttgaccgc gtccatgccg ccctccttcc g
//
LOCUS       DATCCH010000219         5153 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_522281, whole genome shotgun sequence.
ACCESSION   DATCCH010000219 DATCCH010000000
VERSION     DATCCH010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5153
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522281"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..780)
                     /locus_tag="VIJ44_04290"
     CDS             complement(181..780)
                     /locus_tag="VIJ44_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165153.1"
                     /translation="MLASINPLGERARRTRWGRTVVWYTLGSAAGGLVIGAGSAVVGT
                     GLRRLASPSPSVVGLVVAGACAIGLLLDLGVGGAGVLSRRRQVNENWLGHYRGWVYGT
                     GFGFQLGTGVATTVTTSAVYVMLVLAVLSGSVAHALLIGAVFGVVRAVPILTVGRAHD
                     PERLRAVLSRAHAIAGVSRFAAAGGLFLVAAASLAVVVG"
     gene            complement(864..1628)
                     /locus_tag="VIJ44_04295"
     CDS             complement(864..1628)
                     /locus_tag="VIJ44_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165154.1"
                     /translation="MAVLTVLLAVVVALLGVLVAGLLRSHAEILRALHELGVSLDPEE
                     PGPPRHGVEVPRRGEHTRMATDLLGVSPDGDALSVSVVGVEHPTLVAFLTSGCSTCAE
                     FWSALDDVRRRRVPGDARLVVATKGPEAESPGRLRKFAPSDVPVVMSSPAWDDFDVPV
                     APYFAYIDGPSGAVVGEGAASTWEHLQSMLEQALADAGMPTGRRRHRRGSSNRDREAR
                     ADAELRRSGIEPGDPSLYPQTEADLRGPSAGPSMPR"
     gene            1977..2741
                     /locus_tag="VIJ44_04300"
     CDS             1977..2741
                     /locus_tag="VIJ44_04300"
                     /inference="COORDINATES: protein motif:HMM:NF042511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6544 family protein"
                     /protein_id="HEY5165155.1"
                     /translation="MGATHRCVQTPHRLGEGDQGVSTSRVTQLEARLAQPATPGAFSE
                     SELDGLPPAVRRYFSAAIAPGTPLALSARLRMRGTIKIGRWLPFRAREVLAPHQGFVW
                     PARVAGVIGGADGYADGDGRMEWKLAGLVTLAHADGPDVSRSAAGRGGAEAVWVPTSL
                     LPRYGVTWSASDETQIIAGFQVDDTPIEAHYQLDPDGRPRSIVFDRWGDPDNTGTWAW
                     HRCGGEFSAYATFDGVTIPSEGRFGWALRNGPMGRR"
     gene            complement(3054..4034)
                     /locus_tag="VIJ44_04305"
     CDS             complement(3054..4034)
                     /locus_tag="VIJ44_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006336227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165156.1"
                     /translation="MSAPKQPSPDSAVWKGEYRRYDLVKEFVIALVVVSLLTVLLAGV
                     FSSSDKKAVTIAQWSRSDPTDFVTTGLSELDGTSTTAMYGPPYSHTSGAGQKIGPISL
                     QRLAGVRIPVDAPTDLVLRPLETLAPDRPDLRSALGTYRAATSAKQQHWADAYAKALA
                     HAQFRNGQLNVPPGDYGPVSPMMAGLLGFARSGGLDGALVTHAQFYATDYTKPLLFMA
                     DSTYLAGLAQQDHLLGGQWGMTNETGNYPGQPWLWLYTFWYQISPFKTSANADALIWS
                     IMAILTLVLVLVPFIPGLRSIPRRVPVYRLIWRDYYRSVERPRAGPTTEA"
     gene            complement(4031..4708)
                     /locus_tag="VIJ44_04310"
     CDS             complement(4031..4708)
                     /locus_tag="VIJ44_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006336228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEY5165157.1"
                     /translation="MTTPVQPGETEGSSWTGALRRAGLRALPPEQLLPDEQPVYVASW
                     VYVFGVATIAALFVVIGSGLLLAVEGPTWWHASSVGLFFNSLHLWSVELFFFFMVIHL
                     WAKFMMAAWRGRRAMTWVTGAVAFVVSIGAAFTGYLSQQNFSSQWIASQAKDGLNSVG
                     IGAFFNVLDFGQMFTWHVVLLPLGIVALVVVHVLLVRKHGVVPPFPAKTDTRSQPPSP
                     LSTVRDS"
     gene            complement(4705..4875)
                     /locus_tag="VIJ44_04315"
     CDS             complement(4705..4875)
                     /locus_tag="VIJ44_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165158.1"
                     /translation="MIWSAVETITAVGLNGRAYYIHWGWFQISAANLAVIVAMVVVFA
                     LAIVVPFRRRDR"
BASE COUNT          895 a         1822 c         1719 g          717 t
ORIGIN      
        1 gggatcgacg cgacgtgcac gaccgggaag atctgttccc cggccggtgc tggcgcgcct
       61 gggacctcgg cctgggcgct gagctcgccg gcctgccggc ggcggaagat ccggccctcc
      121 catcccggag gtgtctgcac cgcgaggccg tgagcctgga tctgaggcat cagccgcggc
      181 tcagccgacc acgaccgcga ggcttgcggc cgccacgagg aacaagccac cggccgctgc
      241 gaaccggctc acacccgcga tcgcgtgcgc gcgactcagc accgcgcgga ggcgctccgg
      301 atcgtgggcc cgacccacgg tcaagatcgg caccgcccgg accaccccga acaccgcgcc
      361 gatgagcagc gcatgcgcga ccgatccgct caacacggcc aggaccagca tgacgtacac
      421 cgccgaggtg gtgacggtgg tcgcgacacc cgtcccgagc tggaagccga agccggtccc
      481 gtacacccag ccccggtagt ggccgagcca gttctcgttg acctgtcgcc gacgggacag
      541 cacaccggcg cccccgaccc cgagatccag cagcaatccg atcgcgcacg cgccggccac
      601 gacgagccca accacgctgg gagacggcga ggccagccgt cggagtccgg tgccgaccac
      661 cgcggatccc gcgccgatca cgagcccgcc cgcggccgag cccagcgtgt accagacgac
      721 cgttcgcccc caccgggtcc ttcgggcccg ctccccgagc gggttgatgc tcgcgagcat
      781 cgactcccct catggcgacc acgacgaccg cagcgcggcg atcacggcga cgacgacgcc
      841 cgcgacggtc acctcgacgg gagtcaccgc ggcatcgacg ggccggccga cggaccgcgc
      901 agatcggctt cggtctgtgg gtagaggctg gggtcacccg gctcgattcc cgaccggcgg
      961 agctcggcgt cggctcgggc ttcgcgatcc cggttgctgg aaccccgccg gtgtcggcgc
     1021 cgacccgtcg gcatcccggc atcagcgagg gcctgctcga gcatgctttg caggtgctcc
     1081 cacgtcgatg ccgccccctc acccacaacg gccccgctcg gaccgtcgat gtaggcaaag
     1141 taaggggcga cggggacgtc gaagtcatcc catgcggggc tcgacatgac gacgggaacg
     1201 tctgacgggg cgaacttccg cagccggccc gggctctcgg cctcggggcc tttcgtggcc
     1261 accaccaacc gtgcgtcacc aggaacgcgg cgtcttcgga cgtcgtcgag cgccgaccag
     1321 aactcggcac acgtggagca cccgctggtc agaaaagcga cgagggtcgg atgctcgaca
     1381 cccacgacac tcacgctgag cgcatcaccg tccggggaaa cgccgaggag gtcggtcgcc
     1441 atgcgggtgt gctcaccccg tcggggcacc tcgacgccgt gccgcggcgg gcccggctcc
     1501 tcagggtcga gactgacgcc gagctcgtgc agtgcccgca ggatctcggc gtggctccgc
     1561 aacagccccg cgaccagcac ccccagcagg gcgacgacca ccgcgagcag caccgtgagg
     1621 acagccacgg tcatgattgt cgcaggccac ctcggtcgct gccgacatgg ggggccggat
     1681 cacctccgaa accagcggac tcggaggtct cgtcggcgcc cggcgaggga gaacggggag
     1741 aaatgcgacg tttggaccca cccggatcgg ccagaactgg ccgaatgagt tgcgagaatc
     1801 cccagaaccc ccatcatgac ccccacccga ccgagcagac gggagggcca caccgcggcc
     1861 cgggcaggga tcccaacctg gcgacagggc accaggtacc gacgggcctc tggaggcctc
     1921 ggatcctccg ctcgtttgcg tcggaccgga cgtgattttt gcaggaacgg tcaggaatgg
     1981 gcgcgacgca ccgatgcgtt cagacacctc atcgcctggg tgagggggat cagggagtga
     2041 gcacctcgag ggtcacacag cttgaggcac gcctggcgca gcccgctacg ccaggagcgt
     2101 ttagcgaatc ggagctggac ggtctccccc cggcggtgcg gcgctacttc tcggcggcga
     2161 ttgcacccgg gacaccgctg gcgctatcgg cccgactccg aatgcggggc acgatcaaga
     2221 tcgggaggtg gctccccttc cgggcccgag aggtgctggc accccaccag ggcttcgtgt
     2281 ggccggcccg cgtcgccggg gtcatcggcg gcgccgacgg ttacgccgac ggggacggcc
     2341 gcatggagtg gaagctcgcg ggcctcgtca ccctggccca cgccgacggt cccgacgtct
     2401 cgcgcagcgc ggccggccgc ggtggcgccg aggcggtctg ggtaccgacc agcctcctgc
     2461 cccgctacgg cgtcacctgg tcggcgagcg acgaaaccca gatcatcgcc ggctttcagg
     2521 tcgacgacac gccaatcgag gcccactacc aactggaccc ggacggacga ccccgctcga
     2581 ttgtcttcga ccgatggggc gaccccgaca acacgggaac ttgggcttgg catcgctgcg
     2641 gcggcgagtt cagcgcctac gcgaccttcg acggcgtcac catcccgagc gagggccgct
     2701 tcggctgggc actacggaac ggaccgatgg gccgacggtg aattcttccg gtatcgaatc
     2761 acccacttga cgttggcgac ttgcgcctcc tgaggtcaca cgaaatgagc ggaggctcgg
     2821 ggctggcgga cccgcgatcg ccgttcgctc ttcggcccgg cgtggcacga ggacaccatc
     2881 gtcgaccgcc tcgacaccgg ctttcaccac gcggccgcga gcaggatccc ggcgcgggcc
     2941 cggttggata gcagtcggag ttgcgcaccc gccgctggac cgtcccgttg acgagccccc
     3001 accgcttgcg cggaatacgg cctcgcctcc ctgcggagcg gcgcgtcgcg gtctcaggcc
     3061 tcggtggtcg gcccggcgcg tggacgctcc acgctgcggt agtagtcgcg ccagatcagc
     3121 cggtagacgg gcactcgacg cggaatcgag cgcaggcccg ggatgaacgg cacgaggacg
     3181 aggacgaggg tgaggatcgc catgatcgac cagatgagcg catccgcgtt ggctgacgtc
     3241 ttgaacggag agatctggta ccagaacgta tagagccaca gccatggctg accgggatag
     3301 ttaccggtct cgttcgtcat tccccattgc ccgccgagga ggtgatcctg ttgggccagg
     3361 cccgcgaggt aggtgctgtc agccatgaac agcagcggct tggtgtagtc ggtggcatag
     3421 aactgcgcgt gcgtcaccaa cgccccgtcg aggccaccgc tgcgcgcaaa tccgagcagg
     3481 cccgccatca tcggcgagac gggcccatag tcgccgggtg gcacgttcag ctgaccgttg
     3541 cgaaactgtg cgtgggcgag cgccttggcg tacgcgtccg cccaatgctg ctgcttcgcg
     3601 ctggtcgctg cccggtaggt gcccaaggca cttcggaggt cgggtcggtc tggggccaag
     3661 gtctcgagcg ggcgaagcac caagtccgtt ggagcgtcga ccgggattcg gaccccagcg
     3721 agacgctgca gcgaaatcgg cccgatcttc tggccggcgc ccgacgtgtg gctgtagggc
     3781 ggcccgtaca tcgcggtcgt gctcgtcccg tcgagctctg agagccctgt cgtcacgaaa
     3841 tcggtcgggt cactgcggga ccattgcgcg atcgtcaccg ccttcttgtc cgaggacgag
     3901 aacaccccgg cgaggaggac ggtcagcagg ctcaccacta ccagcgcgat cacgaactcc
     3961 ttgacgaggt catagcgtcg atactcgccc ttccacaccg ccgagtctgg cgacggctgc
     4021 ttcggcgcgc tcatgagtcc cgcaccgtcg agagaggcga tgggggctgc gacctcgtgt
     4081 cggtcttggc tgggaagggg ggcaccacgc cgtgcttccg gaccagcagc acgtgaacga
     4141 ccacgagggc cacgatcccg agcgggagca ggaccacatg ccaggtgaac atctgcccga
     4201 agtcgaggac gttgaagaat gccccgatcc cgaccgagtt cagcccgtct ttggcttggg
     4261 aagcaatcca ttgcgacgag aagttctgtt gggacagata tccggtgaac gcggcgccga
     4321 tcgacacgac gaatgccacc gcacccgtca cccatgtcat cgcccgtcgg cctcgccacg
     4381 cggccatcat gaacttcgcc cagagatgga tgaccatgaa gaagaagaac agctcgacac
     4441 tccacaagtg caggctgttg aagaagagac ccactgagga tgcgtgccac cacgtcggac
     4501 cctcaactgc gagcaggagt cccgatccga tcaccacgaa cagcgccgcg atcgtcgcca
     4561 ctccgaagac gtacacccac gatgcgacat acaccggctg ctcgtcagga agcagctgct
     4621 cgggcggcaa cgcccgcagg ccggcgcgtc gcagcgcgcc ggtccacgac gacccttcgg
     4681 tctcccctgg ttgaaccggc gtggtcatcg gtcacgcctc cgaaacggca ccacgatcgc
     4741 caacgcgaac accacgacca tcgcgacgat cacagccaga ttcgcggcgg agatctgaaa
     4801 ccagccccag tggatgtagt acgcccgtcc gttgaggccg accgccgtga tcgtctcgac
     4861 cgcgctccag atcatcggct ctaccttttg ctctgggagc cgggttgagg ccagcccgtt
     4921 ggggtcgccg tcgtccggga ccacgccagg tcaggccccg acgacgctct gacacccgcc
     4981 tgtcgacacc gacgcggcgc cgtgccgtgc ttcgtcctcg acgaccagca cgaggcgtcg
     5041 agcgaggatc gggtggacat ctcgcccacg ggcctggcct ccacggcgcc ggcgagcctg
     5101 ccgatcacgc cgatccgagg gtccaagtcc ccgcggaccg ggaccagcgc gag
//
LOCUS       DATCCH010000220         3192 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_190404, whole genome shotgun sequence.
ACCESSION   DATCCH010000220 DATCCH010000000
VERSION     DATCCH010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190404"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..227)
                     /locus_tag="VIJ44_04320"
     CDS             complement(60..227)
                     /locus_tag="VIJ44_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165159.1"
                     /translation="MGHDEITAQLTGLIDTALETYRGRDLVAASEVTDLLLDLRLMLL
                     AADEVAVTPAG"
     gene            complement(349..840)
                     /locus_tag="VIJ44_04325"
     CDS             complement(349..840)
                     /locus_tag="VIJ44_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEY5165160.1"
                     /translation="MAGLEDLYREIILDHYRAPRNRGELPAPPARMVEGFNPLCGDEV
                     VLYLDVDDDGVLRDVRTGGQGCSISQASTSMMSAAVKGQSLPEARRLIRAFKALMSIH
                     ESKLEGETDGEDGAELEGVRLGDLEALQGVVKFPVRIKCATLAWNTLEQGLDESDTST
                     GPA"
     gene            complement(854..2086)
                     /locus_tag="VIJ44_04330"
     CDS             complement(854..2086)
                     /locus_tag="VIJ44_04330"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufS family cysteine desulfurase"
                     /protein_id="HEY5165161.1"
                     /translation="MTLDVAGVQADFPILKRQVHGRRLVYLDSASSSQKPNAVLDAMD
                     HLYRESYANVHRGVYQIAEEATAAYEGARARLARFLGAASASEIVFTRNVTEAINLVA
                     YSWGRANLRAGDVVVVTELEHHANVVPWHILAAERGIQLRWVPLGKDFRLDLSGLDEL
                     LDGAKLFAFSAASNVLGTITDVRFLADAAHAAGALALVDAAQFAPHRRTDVAAWDADF
                     VGVTGHKMLGPTGIGALWARPELLEAMPPFLGGGEMIRDVRQDGFIPNDVPWKFEAGT
                     MPIAEAVGLAAAIDYLDGLGMDDVAAHEVALTRYALRALEERFGDRLAIYGPADMERR
                     GATISFLFEGIHAHDISQVVDEHGVCVRAGHHCAKPLMRVLGVPATTRASFYVYNDEA
                     DVDALVDALAAAERFFAI"
     gene            complement(2097..2882)
                     /gene="sufC"
                     /locus_tag="VIJ44_04335"
     CDS             complement(2097..2882)
                     /gene="sufC"
                     /locus_tag="VIJ44_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014630312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEY5165162.1"
                     /translation="MSAPPAPVPPALRIEDLHVAVDGNEILRGVSLAVGAGELHALMG
                     PNGSGKSTLAKTLLADPGYQVTTGRILLHDEDVTALSTADRAARGLFLGFQHPAEIPG
                     VSVLNFLRQAMAARQGIPDLSVLEVRMALLEWTKRLGTDDRFAERYLNEGFSGGEKKR
                     NEILQMALLEPDVAVLDETDSGLDIDALHVVARGVEAVRERRPALGILLITHYRRILE
                     HLTPDRVHVLLHGRIVASGGPEIADRVEREGFDGIRREAEAAA"
     gene            complement(2901..>3192)
                     /locus_tag="VIJ44_04340"
     CDS             complement(2901..>3192)
                     /locus_tag="VIJ44_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165163.1"
                     /translation="TARIAALGAEARARLDGVAGWRAQEPLDEPSGIVTLAHPDRDPM
                     AVARDLLGQGIVTSAIPVTRALGDLAGPLLRVSLHAYCNPEDLDPLEFSLRR"
BASE COUNT          473 a         1070 c         1153 g          496 t
ORIGIN      
        1 gccatcggcg gccagactac aaagtgccgc cgcgagctcg ggctccggtc ggagccgggc
       61 tacccggcgg gggtgaccgc aacctcgtcc gcggcgagca gcatgaggcg caggtcgagc
      121 aggagatcgg tgacctcgga cgcggcgacg aggtcgcgac cgcggtaggt ctcaagggcc
      181 gtgtcgatca ggccggtgag ctgtgcggtg atctcgtcgt ggcccattca gccctcctgg
      241 gtctcctggc gctccctgtc agcaaggtca acctagcgcc ccgttgtttc atgcccgtga
      301 caccccgatg aacgctgtga ctcccggcac gacgggggct ctggggggtc aggccgggcc
      361 ggtcgaggtg tcggactcgt cgaggccctg ttccagggtg ttccaggcca gcgtggcgca
      421 cttgatccga accgggaact tgacgacgcc ttggagggcc tcgaggtcgc cgaggcggac
      481 cccctcgagc tcggcgccgt cctctccgtc cgtttcgcct tcgagcttcg attcgtggat
      541 cgacatcagc gccttgaagg cccggatcag ccgacgggcc tcggggagcg actggccttt
      601 caccgcggcg ctcatcatcg acgtcgatgc ttggctgatc gagcagccct ggccgcctgt
      661 tcgcacgtca cggagcaccc cgtcgtcgtc gacatcgagg tacaagacga cctcgtcgcc
      721 gcacagcggg ttgaagccct cgaccatgcg cgccggtggg gcgggcagct caccgcgatt
      781 tcgcggtgcc cgatagtggt cgaggatgat ctctcggtac aggtcctcga gacccgccat
      841 gtcgtcggct cctttagatc gcgaagaaac gctcggcggc cgcgagcgcg tcgacgagcg
      901 cgtcgacgtc ggcttcgtcg ttgtagacgt agaacgacgc ccgggtggtg gcggggacgc
      961 cgaggacccg catcaacggc ttcgcgcagt gatgcccggc ccggacgcag accccgtgct
     1021 catcgacgac ctgggagatg tcgtgggcat ggatgccctc gaacaggaac gagattgtcg
     1081 ctcctcggcg ttccatgtcg gcgggaccgt agatcgccag acggtccccg aaccgttcct
     1141 cgagcgcccg gagcgcgtag cgggtgagtg ccacctcgtg ggccgcgacg tcgtccatcc
     1201 caagcccgtc gaggtagtcg atcgccgccg cgaggccgac cgcctcggcg atcggcatcg
     1261 tgccggcctc gaacttccag ggaacgtcgt tggggatgaa cccgtcctgg cgcacgtcgc
     1321 ggatcatctc gccgcccccg aggaacggtg gcatcgcctc gaggagctcg gggcgcgccc
     1381 acagggcgcc gatgccggtc gggccgagca tcttgtggcc ggtgacaccg acgaagtcgg
     1441 cgtcccacgc cgcgacgtcg gtgcgtcggt ggggggcgaa ttgcgccgcg tcgacgagcg
     1501 cgagggcccc ggcggcgtgc gcggcgtcgg cgaggaagcg gacgtcggtg atggtgccga
     1561 ggacgttgga cgcggcggag aacgcgaaca gcttggcgcc gtcgagcagc tcgtcgagcc
     1621 cggacaggtc gagtcgaaag tccttcccga gtggcaccca gcggagctgg atgccccgtt
     1681 cggcggcgag gatgtgccac gggacgacat tggcatgatg ttccagctcg gtgaccacga
     1741 ccacgtcgcc ggcgcggagg ttggcccgtc cccaggagta ggcgacgagg ttgatggcct
     1801 ccgtgacgtt gcgggtgaac acgatctccg acgcggacgc cgcgccgagg aaccgtgcga
     1861 ggcgcgctcg ggctccctcg tacgcggcgg tggcctcctc ggcgatctgg tacacgcctc
     1921 ggtgcacgtt ggcgtacgac tcgcggtaga ggtgatccat ggcgtcgagt accgcgttcg
     1981 gcttctgtga cgacgacgcc gaatcgaggt acaccaggcg ccgcccgtgc acctggcgct
     2041 tcaggatcgg gaagtccgcc tggaccccgg ccacgtcgag ggtcatcgcc gactcctcac
     2101 gccgcggctt ccgcttcgcg gcggatcccg tcgaagccct cccgctcgac gcggtcggcg
     2161 atctcgggac caccggaggc gacgatccgg ccgtgcaaca agacgtgcac ccggtcgggc
     2221 gtgaggtgct cgaggatcct ccggtagtgg gtgatcagca ggatgccgag ggccgggcgg
     2281 cgttcgcgga ccgcttcgac ccctcgagcg acgacgtgca gcgcgtcgat gtcgagaccc
     2341 gagtcggtct cgtcgaggac cgcgacgtcg ggctcgagga gcgccatctg caggatctcg
     2401 ttgcgtttct tctcaccgcc ggagaagccc tcgttcaggt accgctccgc gaaccggtcg
     2461 tcggtcccga gccgcttggt ccactcgagc agcgccatgc ggacttcgag gaccgacagg
     2521 tccgggatcc cctgacgggc ggccatcgct tggcgcaaga agttcaagac gctgaccccg
     2581 gggatctcgg ccgggtgctg gaagccgagg aacaatcctc gggcggcccg gtcggcggtg
     2641 ctgagcgcgg tgacgtcctc gtcgtgcagc aggatccggc ccgtcgtcac ctggtagccg
     2701 ggatcggcca ggagggtctt ggcgagggtc gatttgccgg acccgttggg ccccatgagg
     2761 gcgtggagct ccccggcgcc gacggccagc gagacgccac gcaggatctc gttcccgtcg
     2821 accgcgacgt ggagatcttc gatgcgcaac gccggcggca cgggcgcagg gggggcgctc
     2881 accaccccat tgtattctca ctaccgccgt agtgaaaact ccaaggggtc gagatcctcg
     2941 gggttgcagt aggcgtgcaa cgagacccgc agcagcgggc cggcgagatc ccccaacgcc
     3001 cgggtgaccg ggatcgctga cgtcacgatc ccctggccga ggaggtctcg ggcgaccgcc
     3061 atcgggtccc ggtcggggtg ggccagcgtc acgatccccg acggctcgtc gagcggctct
     3121 tgggcccgcc agccggcgac cccgtcgagg cgggctcggg cctcggcgcc gagggccgcg
     3181 atccgggcgg tc
//
LOCUS       DATCCH010000221         4473 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_344280, whole genome shotgun sequence.
ACCESSION   DATCCH010000221 DATCCH010000000
VERSION     DATCCH010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344280"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..2255
                     /locus_tag="VIJ44_04345"
     CDS             51..2255
                     /locus_tag="VIJ44_04345"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY5165164.1"
                     /translation="METLVLGRLATWSYRHRRQVLVLWLVALVGVIALGRSFGGDTTT
                     SFTLPNVESQQAFALLQSRFPSRAGDSAQIVFATPAGVQSPAVRAQVAQLLGRLAHLP
                     HVTGVVNPYSGQGRRISTDGTIAFATIQFDQRARDLPNSVGQQLVDEVQTANGHGVQV
                     EAGGSVAQAAVRPPPPSSELLGLLAAILILLIAFGSVIAMGLPILTALFGLGIGLGGL
                     PLFALVFDMPTFAAQLAAMIGLGVGIDYALFIVTRYRQELHAGKGPEDATRTALITSG
                     RAVVFAGCTVIISLLGMLLMGLSFVQGLAIGAVTAVLLVMLASVTLLPAVLGFTGRAI
                     DKLRVPGLHRNESAHRTSVAFRWSRQVQRHPWRYALGGFTVLILLCVPVLSLRLGTSD
                     AGNDPTSLTTRRSYDLLSKGFGPGFNGPLVVAAQLPDPAARSVAQALWVRLEHTAGVA
                     GVTPLQVSPRRDAAVITVYPANAPQDARTTSLVHRLRDTVVPHVTNGTGVVVRIGGTT
                     ASGIDLSEFLGGRLPWFIGAVIALSFVLLMVVFRSLVVPLKAAIMNLLSIGAAYGIVV
                     AVFQWGWFGSIVGINKSGPIDAFVPMMLFAVLFGLSMDYEVFLLSRIREEYGRTRDNA
                     AAVADGLAATARVITAAAAIMISVFLAFVLGDTRVIKLFGLGLASAIFIDATLVRMLL
                     VPATMELLGDANWWLPRWLSRLLPDLDVEGTHHGVEGPVTPEPEPVPVSSPS"
     gene            complement(2281..3063)
                     /locus_tag="VIJ44_04350"
     CDS             complement(2281..3063)
                     /locus_tag="VIJ44_04350"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY5165165.1"
                     /translation="MVVGSRSFDRAAPFYDRTRGLPAEATARVTELLVGELTGRGRCL
                     EIGVGTGRIAWPLHEAGLPLTGIDLSRPMMSVLVEKAGGRVPFPLAQGDATALPFPDH
                     RFGAVLASHVFHLIPEWRRAITEVLRVVRPGGVLLSSRGGRDRDGVLGQVRAEFQGRL
                     GVEAVNVGVAHASREVDDTLAEMGARERRLPPVRATRTTTVGSLIDSLEAGQWSWSWG
                     LAGDRLREAGHSTREWARSRFGPLEEPVAVEVEVRWTAFDLG"
     gene            complement(3118..3486)
                     /locus_tag="VIJ44_04355"
     CDS             complement(3118..3486)
                     /locus_tag="VIJ44_04355"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5165166.1"
                     /translation="MKITRILHASVNVEDRLEPTAAFYTTILGLDPAPRPTIPGIAGQ
                     WFSVSDAQLHLVDAPHRGAPRIDPTGPHICFGVEDLDGAVAELDGAGIPYERGTQGAI
                     TQIWITDPSGFTIELQLDPA"
     gene            3576..>4473
                     /locus_tag="VIJ44_04360"
     CDS             3576..>4473
                     /locus_tag="VIJ44_04360"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY5165167.1"
                     /translation="MDRVRMGIVGVGNVAPLNVAGYLDHDRCDVVALCDPREDKARQM
                     ATEWDVPTVYTSLDDLLADDRVDAVEILTPTHLHKEHVLAAVRAGKHVSCQKPIANSV
                     ADGIEMTEAARDAGVVFRVSECFFHYPPLVKAKELIASGAIGAPTMLRVKTVVGTTDT
                     EFQRHLEPQGYVWRFNAQSPGGHLFDDIVHKYATALWLFDQDITSVQAVVRQAPIFFE
                     APTAAIWEYERETLLGMMEVTYAPNMSLRSSYYGADEFFEIQGDEGFIWVTRCTGEML
                     DLAPVLLFQGKGSNGSMREIPVV"
BASE COUNT          646 a         1591 c         1488 g          748 t
ORIGIN      
        1 gaggttctag tctcgagccg cccggttccg ggttcccccc gtgcccgtag atggagaccc
       61 tcgtgcttgg tcgtctcgcg acgtggagtt atcggcaccg gcgccaggtg ctggtgctct
      121 ggctcgtcgc cctggttggc gtcatcgcgc tcggtcgcag cttcggcggc gacaccacga
      181 cgagcttcac cctgcccaac gtcgaatcgc agcaggcgtt cgcgctgttg cagtcgcggt
      241 tcccgagccg tgccggcgac tcggcgcaga tcgtcttcgc gactcccgcc ggggtccagt
      301 cccccgcggt gcgcgcgcag gtggcgcagc tcctcgggcg gctcgctcac ctcccgcacg
      361 tcaccggtgt cgtcaacccg tactcgggcc aagggcggcg gatctccacc gacgggacga
      421 tcgcgttcgc caccatccaa ttcgaccaac gggcccggga cctacccaac tcggtcggcc
      481 agcagctcgt cgacgaagtc cagaccgcca acggccacgg cgtccaggtc gaggcgggcg
      541 gcagcgtcgc ccaggcggcg gtccgcccac cccctcccag cagcgagctg ctcggcctgc
      601 tcgcggcgat tctgatcctc ctgatcgcgt tcgggtccgt catcgcgatg gggttgccga
      661 tcctcaccgc gctcttcggg ctcggtatcg gactcggcgg tctcccgctc ttcgcgttgg
      721 tcttcgacat gccgaccttc gccgcgcagc tcgccgcgat gatcggactc ggcgtcggca
      781 tcgactacgc cctgttcatc gtcacccgct accgccagga gctccacgcc gggaagggac
      841 ccgaggacgc cacccgcacc gccctcatca catcggggcg cgcggtggtg ttcgccggct
      901 gcacggtcat catctcgctg cttggcatgc tcctgatggg cttgtcgttc gtgcagggcc
      961 tcgcgatcgg ggcggtcacc gcggtcctcc tcgtgatgct ggcatcggtc acgctgctgc
     1021 ccgccgtgct tggcttcacc ggccgggcca tcgacaagct ccgtgtcccc ggcctgcacc
     1081 gaaacgaatc ggcgcaccgt acgtcggtcg cctttcggtg gagccgccaa gtccaacgtc
     1141 acccgtggcg gtacgcgttg ggtggcttca ccgtgctgat actcctctgc gtcccggtgc
     1201 tgtcgctgcg cctcggcacc tccgacgccg gcaacgaccc cacgtcgctg accacccggc
     1261 gctcgtacga cctgctctcg aagggcttcg gtccgggctt caacggcccg ctcgtcgtcg
     1321 cggcccagct tcccgacccg gccgcccgct cggtggcgca ggccctctgg gtgcgtctcg
     1381 agcacaccgc cggggtcgcg ggtgtgaccc cactccaggt gagtccccga cgggacgccg
     1441 cagtcatcac cgtctacccc gcgaacgcgc cgcaggacgc gcggacgact tccctggtgc
     1501 accgtctccg tgacaccgtc gtgccccacg tcaccaacgg gacgggggtc gtggtgcgga
     1561 tcggaggcac caccgcgtcg ggtatcgatc tgtccgagtt cctcggtggt cgactcccgt
     1621 ggttcatcgg cgcggtgatc gcgctgtcgt tcgtgctgct catggttgtc ttccggtcgc
     1681 tcgtcgtgcc tctgaaggcc gcgatcatga acctgctctc gatcggcgcg gcctacggaa
     1741 tcgtcgtcgc ggtcttccaa tggggctggt tcggcagcat cgtcggcatc aacaagagcg
     1801 gtccgatcga cgcgttcgtg cccatgatgc tgttcgcggt gctcttcggc ttgtcgatgg
     1861 actacgaggt gttcttgctg tcacggatcc gtgaggaata cggacggacc cgagacaacg
     1921 cggccgcggt agccgacggg ctcgcggcga cggcgcgggt gatcaccgcc gcggcggcga
     1981 tcatgatctc ggtgttcttg gcgttcgtgc tcggcgatac gcgcgtcatc aagctcttcg
     2041 ggctcggact ggcgagcgcg atcttcatcg acgccacctt ggtccggatg ctgctggtgc
     2101 ccgccaccat ggagttgctc ggcgacgcca actggtggct ccctcgctgg ctcagccgcc
     2161 tgctgcccga cctcgacgtc gagggcaccc atcacggagt cgagggcccc gtcacccccg
     2221 agcccgagcc cgtcccggtc agttcgccga gctgatccac ggctccgacc gaggcggtcg
     2281 tcaccccaga tcgaaggccg tccaccgcac ctccacctcg acggctaccg gctcctcgag
     2341 gggtccgaac cgcgaccgag cccactcccg cgtgctgtgc ccggcctctc ggaggcgatc
     2401 gcccgccagc ccccacgacc acgaccactg acccgcctcg aggctgtcga tcaggctccc
     2461 gaccgtcgtc gtcctcgtgg cccgcaccgg cggcaaccga cgttcccggg cccccatctc
     2521 ggccagggtg tcgtcgacct cgcggctggc gtgggccacc ccgacgttca ccgcctcgac
     2581 accgagccgg ccctggaact cggcccggac ctgaccgagc acgccgtcgc ggtcgcgtcc
     2641 cccgcggctg ctgaggagca cgcccccggg gcgaacgacg cggagcacct cggtgatggc
     2701 gcggcgccac tccgggatga ggtggaagac gtgcgacgcg aggacggcgc cgaaccgatg
     2761 gtccgggaac ggcaaggcgg tcgcgtcgcc ttgcgcgagt gggaacggca cgcggccccc
     2821 cgccttctcg acgaggacgc tcatcatcgg ccgggacagg tcgatcccgg tcaagggcaa
     2881 ccccgcttcg tgcaggggcc acgcgatgcg accggtgccg accccgatct cgagacagcg
     2941 tccccggccg gtgagctcgc cgacgagcag ctcggtgacg cgcgctgtcg cctcggcggg
     3001 caagccgcgg gtccggtcat agaacggcgc ggcgcgatcg aagctgcgag aacccacgac
     3061 catcaccgcc gagtatgacg cgatcctcaa ccgcgaggcc gacgaggcgg cggtcgctca
     3121 ggcggggtcg agttggagct cgatggtgaa cccagacgga tccgtgatcc agatctgggt
     3181 gatggcaccc tgggtgccgc gttcgtacgg gatgccggca ccgtcgagct ccgcgacggc
     3241 gccgtcgagg tcctcgaccc cgaagcagat gtgtggaccc gtcgggtcga tccgaggcgc
     3301 acctcgatgc ggtgcgtcga cgagatggag ctgcgcgtcg ctgaccgaga accactgacc
     3361 ggcgatcccc gggatcgtcg gtcgtggtgc cggatcgagc ccgaggatcg tggtgtagaa
     3421 cgccgcggtc ggttcgaggc ggtcctcgac gttcaccgat gcgtgcagga tccgggtgat
     3481 cttcatggct gcgccttcga gagtcagtgc ggggggagcg ccaggtcgag cacccagacc
     3541 gtactgtggg cgccccacga gcaaggagcc aggcggtgga tcgcgttcgg atgggaatcg
     3601 tcggtgtcgg caacgtcgcg ccgctcaacg ttgcggggta tctcgaccat gaccgatgcg
     3661 acgtcgtcgc gctctgtgat ccccgcgagg acaaggcccg acagatggcg accgaatggg
     3721 acgtcccgac ggtctacacg tcgctcgacg atctgctggc cgacgaccgg gtcgacgcgg
     3781 tcgagatcct caccccgacc cacctccaca aggagcatgt gctcgccgcg gtgcgcgccg
     3841 gaaagcacgt gtcgtgccag aagccgattg cgaacagtgt ggccgacggc atcgagatga
     3901 ccgaagccgc ccgcgacgcc ggggtggtgt tccgggtcag cgagtgcttc ttccactacc
     3961 ccccgttggt gaaggccaag gagctgatcg cgtctggtgc catcggtgca ccgacgatgc
     4021 tgcgagtcaa gaccgtcgtc ggtacgaccg acaccgagtt ccaacgtcat ctcgagcctc
     4081 agggctacgt gtggcggttc aacgcccaga gccccggcgg tcacctcttc gacgacattg
     4141 tgcacaagta cgccaccgcc ctgtggctct tcgatcagga catcacgagc gtccaagccg
     4201 tggtgcgtca ggcgccgatc ttcttcgagg cgcccaccgc ggcgatctgg gagtacgaac
     4261 gcgagacgct gctcggcatg atggaagtca cctacgcgcc gaacatgtcc ttgcggagca
     4321 gctactacgg cgccgacgag ttcttcgaga tccagggcga cgaggggttc atctgggtga
     4381 cccggtgtac cggcgagatg ctcgacctcg ctcccgtcct gctcttccag gggaagggaa
     4441 gcaacgggtc gatgcgggag atccccgtgg tcg
//
LOCUS       DATCCH010000222         2181 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_314027, whole genome shotgun sequence.
ACCESSION   DATCCH010000222 DATCCH010000000
VERSION     DATCCH010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314027"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VIJ44_04365"
     CDS             <1..297
                     /locus_tag="VIJ44_04365"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY5165168.1"
                     /translation="RPAARRGVLARHGPGPRPRCLHHRRPPYRVVFSWGIPGSDTLPP
                     GSSTVEVGLTPDGDDTMVVLTHRGLPSTHIGDHRAGWEHQLGRLPAAAGRPRCS"
     gene            685..1446
                     /locus_tag="VIJ44_04370"
     CDS             685..1446
                     /locus_tag="VIJ44_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009155478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY5165169.1"
                     /translation="MEPVFKALADPTRRELLDELFRHDGQTLGALAGRFAMTRVAVAK
                     HLRLLEDAGLVATRRRGREKLHYLNPVPIRLVHDRWVNKYTEAWAAGLVDLKRDLEAS
                     VEKVFEIYIQTTPERLWQAITDPKIRTRYQFGAQIESDWAPGSRYQVAHAGAAGPLIE
                     GENLEIDPPRRLVQSYHAVWDDEIAAEGTSRVTWVIEPVGDSCRLTVTHDQLPEDADE
                     HLYGGWPMILSGLKTWLETGQELTTPGSLNYGGSQ"
     gene            1452..1742
                     /locus_tag="VIJ44_04375"
     CDS             1452..1742
                     /locus_tag="VIJ44_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004003949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HEY5165170.1"
                     /translation="MGKSFTAVLQRSPNRGGWTYVVMPGSAEYSGPRGLVRIRGSIDG
                     HPFRGSFMALGDGTHKLAVPADVRTQIGKDTCDTITVRLDERIPPAPRRSTR"
     gene            1739..2119
                     /locus_tag="VIJ44_04380"
     CDS             1739..2119
                     /locus_tag="VIJ44_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020075036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5165171.1"
                     /translation="MSATKTHITGVRTVSIPVEDQDAALRFYVDTLGFTALRDNPTPN
                     GGRWIELAPGTDNVIVTLEPAQPDVTRGAIGIRFTTEDAEAAHAALQAAGVGTDDILR
                     WPGVPAMFAFRDPDGNAFSISETA"
BASE COUNT          373 a          795 c          697 g          316 t
ORIGIN      
        1 cgacccgcag cccggcgggg tgttctcgct cgacatgggc caggccctcg cccgcggtgc
       61 ctacatcacc gtcgaccgcc gtaccgagtc gtgttcagct ggggcatccc cggcagcgac
      121 acgctgccgc ccggcagttc gaccgtcgag gtcgggctga cccctgacgg cgacgacacg
      181 atggtcgtgc tcacccaccg cggcctgccg tccacccaca tcggcgacca ccgcgccggc
      241 tgggagcacc agctcgggcg cctgcccgct gcggccggcc gaccccggtg cagctgacca
      301 agccggtcct cgaccgtgcg gcgcgtcacc ttctcgggcc cgtcgatgtc ggggtggtgt
      361 tcgccgaggt aggccaacga attggcgggt tccccggatg taggtcgtga ccgtggaggc
      421 cgtcgcgcga tgccgcagtc tgagcgtcca gtcgcggatc ccatgcgggc agccgtgaga
      481 atgcccggtg acggggcgtt ctatgagaca agcatgatca tcggccaatt cccgcgcgcc
      541 gaagcccggc gacctgctgt cgctgttcgg ccaaggggac ccgccagtcg ctgtgtagcg
      601 ccctacccgg acgcataggt gcaatgtcga cgccgtcctt acagcgtttg acatgaaact
      661 aaatggtttc ctatgctcgc ccgcgtggaa ccggtgttca aggcgctggc ggacccaacg
      721 cggcgtgaac tgctcgacga gctgttccga cacgacgggc agacgttggg cgcgctggcg
      781 ggccggttcg ccatgacgcg agtggcggtg gccaagcacc tgcggcttct ggaagacgcc
      841 ggtctggtcg cgacccgccg tcgaggccgg gagaagctgc attacctcaa cccggtcccg
      901 atccggctcg tccacgaccg gtgggtgaac aagtacaccg aggcctgggc ggccggcctg
      961 gtggatctca aacgcgacct ggaggcgagc gtggagaagg tattcgagat ctatatccag
     1021 accacccccg aacggctgtg gcaggcaatc accgacccca agatccggac gcgctaccag
     1081 ttcggcgctc aaatcgagtc cgactgggct cccgggtcgc gctaccaggt cgcacacgcc
     1141 ggtgcggctg gcccgctcat cgagggcgag aacctcgaga tcgacccgcc gcgccggctg
     1201 gtgcagtcct accacgcggt gtgggacgac gagatcgcag ccgagggcac cagccgcgtc
     1261 acctgggtga tcgagccggt tggcgactcg tgccggctga ccgtcaccca tgaccagctg
     1321 cccgaggacg ccgacgaaca cctttacggc ggctggccga tgatcctgtc cgggctcaag
     1381 acctggctcg agaccggaca agaactcacc acccccggct cgctcaacta tggaggatcg
     1441 cagtgaccac cctgggcaag agcttcaccg ccgtgctgca acgcagcccg aataggggcg
     1501 gctggaccta cgtcgtcatg cccggctcgg ccgagtactc cggcccccga ggcctggtcc
     1561 gcattcgcgg cagcatcgac ggccacccgt tccgtggctc gttcatggcc ctcggtgacg
     1621 gcactcacaa gctcgccgtc ccggccgacg tccgcaccca gatcggcaag gacacctgcg
     1681 acacgatcac cgtccggctc gacgaacgca tcccacctgc accgcgaagg agcacccgat
     1741 gagcgccacc aagacccaca tcaccggagt ccgcaccgtc agcatcccgg tcgaggacca
     1801 agacgccgcg ctgcgcttct acgtcgacac gctcggcttc accgcgctgc gcgacaaccc
     1861 gacgcccaac ggcggccgct ggatcgaact cgcccccggc accgacaacg tgatcgtcac
     1921 cctcgaaccc gcccagcccg acgtgacgcg cggcgccatc ggcatccgat tcaccaccga
     1981 ggacgccgaa gccgcgcacg ccgcgcttca agccgccgga gtcggcaccg acgacatcct
     2041 ccgctggccc ggagtcccag cgatgttcgc cttccgggac cccgacggca acgccttctc
     2101 gatctccgag accgcctgac cttgcgccgt aggggatcgg gttatgggac gaggtctgag
     2161 aagcgagaag acccagtgcc g
//
LOCUS       DATCCH010000223         2240 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_53450, whole genome shotgun sequence.
ACCESSION   DATCCH010000223 DATCCH010000000
VERSION     DATCCH010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53450"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="VIJ44_04385"
     CDS             complement(<1..576)
                     /locus_tag="VIJ44_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165172.1"
                     /translation="MADRFPGPFEMPTPEGAEGWRDLYAYSTVFSDDRREYEDSMFWF
                     MDGVHTPEVIEPWDATILEFAIIALSQYNTRHYIIPPALGVDIRILNGYMYLSPVGVA
                     DPEEIGSRVPEFMERAGYYFQNWDRLYEAWLPKVRALIEELEAVTFTPLPDREDMAVI
                     TEGRGIGSGYELLASYNRLLDLTLKLWNHHFE"
     gene            complement(676..1758)
                     /locus_tag="VIJ44_04390"
     CDS             complement(676..1758)
                     /locus_tag="VIJ44_04390"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEY5165173.1"
                     /translation="MAHIAWYEDFDPNEMAGLGGKNASLCTMTAAGLPVPPGFAVTAN
                     AYRRVFADTGVDSRLELLMETVDPTSPASVSKAGSEARGMILDLQLPGWLCEPLVEAY
                     GRLGERCNTPDVPVAVRSSATCEDQPDASFAGEHDTYLWVRGADDVVSHVLRCWASLF
                     TDRAITYRRQMGYPDSGAAMSVGIQKMVLPRAAGVAFTLNPLDGDRSQVAIDASWGFG
                     EAVVSGSVTPDNFLVDKVMGAITRRTISPKTIEHRLGDDDHVIEAEVEPERRDSPCLT
                     DDEIKSVAQLARRAEKHYRCPQDVEWALDAQLPDGDNILLLQARPETVWSRKVRKSAT
                     AGLDTYASIVQTLLDPDHHGRARAAG"
     gene            1894..>2240
                     /locus_tag="VIJ44_04395"
     CDS             1894..>2240
                     /locus_tag="VIJ44_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018333877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY5165174.1"
                     /translation="MALPTVPGEAAAVRDPLLGSVRNAARVLRAFSSADQDLGVTELA
                     ARLGLALYELGTNVTEHVDLHQAALPVLTTLRHNTGEMVHVAVLDGLEVIYVERLESH
                     HLLPIFRTVGHRLP"
BASE COUNT          311 a          779 c          789 g          361 t
ORIGIN      
        1 ctcgaagtgg tggttccaca gcttcagggt caggtcgagg agccggttgt agctggcgag
       61 cagctcgtac ccgctgccga tcccacgacc ctcggtgatg accgccatgt cctcgcggtc
      121 cgggaggggc gtgaacgtga ccgcctccag ctcctcgatg agcgcgcgaa ccttgggcag
      181 ccaggcctcg tacagccggt cccagttctg gaagtagtag ccggcccgct ccatgaactc
      241 ggggacccgc gagccgatct cctcggggtc tgccacgccg accggggaga ggtacatgta
      301 cccgttgagg atgcggatgt cgacgccgag cgccggcggg atgatgtagt gccgggtgtt
      361 gtactgcgac agcgcgatga tcgcgaactc gaggatggtc gcgtcccagg gctcgatcac
      421 ctcgggggtg tgcacgccgt ccatgaacca gaacatcgag tcctcgtact cgcgacggtc
      481 gtcgctgaag acggtcgagt acgcgtacag atcccgccag ccctcggcgc cctcaggcgt
      541 gggcatctcg aacgggccgg gaaaccgatc tgccatgggt ggtccttcct gacgccactg
      601 gcctgagccg gcggccacga cggccgacgg gtcgtacggg tggtgcgggc ctacgggtcg
      661 cacgggtcga gctcgctagc cggccgccct cgcacggccg tggtggtcag ggtcgagcag
      721 cgtctgcacg atgctcgcgt aggtgtcgag cccggcggtc gcggacttgc ggaccttgcg
      781 gctccacacc gtctccggtc gcgcctgcag cagcaggatg ttgtcgccgt cgggcagctg
      841 ggcgtccagc gcccactcca cgtcctgcgg gcagcggtag tgcttctcgg ctcggcgggc
      901 cagctgggcc acggacttga tctcgtcgtc ggtcaggcac ggcgagtcgc ggcgctccgg
      961 ctcgacctcg gcctcgatga cgtggtcgtc gtcgccgagc cggtgctcga tggtcttggg
     1021 cgagatcgtg cgccgggtga tcgcacccat caccttgtcg accaggaagt tgtccggcgt
     1081 caccgagccg ctgacgacgg cctcgccgaa gccccagctc gcgtcgatgg cgacctgcga
     1141 acggtcgccg tcgagggggt tgagcgtgaa ggccaccccg gcggcccgcg gcagcaccat
     1201 cttctggatg ccgacgctca tcgcggcgcc actgtcgggg taacccatct gcctgcggta
     1261 ggtgatcgcg cggtcggtga acaggctggc ccagcagcgg agcacgtggc tgacgacgtc
     1321 gtcggccccc cgcacccaca ggtacgtgtc gtgctccccc gcgaagctcg cgtccggctg
     1381 gtcctcgcag gtcgcgctcg agcggacggc gacgggtacg tccggggtat tgcaacgctc
     1441 gccgaggcgg ccgtaggcct cgaccaacgg ttcgcagagc cagcccggca gctggaggtc
     1501 gaggatcatc ccgcgggcct cgctgcccgc cttcgagacg gacgccgggc tcgtcgggtc
     1561 gaccgtctcc atgagcagct cgagccggct gtcgacgccg gtgtcggcga agaccctccg
     1621 gtaggcgttc gcggtgacgg cgaacccggg tggtacgggc aggcccgcgg cggtcatggt
     1681 gcacaggctg gcgttcttgc cgccgaggcc ggccatctcg ttcgggtcga agtcctcgta
     1741 ccacgcgatg tgcgccaagc ccggcctcct cgctggcggg tagtggcggg cagccgaggc
     1801 gacgccccga cgttgccccg acgttacgac cgtgtgacgt gggccacaat gggtctgtcc
     1861 cgcggagcgg gacaggatcg gtaccgtacg gacatggcgc tccccaccgt gcccggcgag
     1921 gctgcagccg tgcgcgaccc gctgctcggc tcggtgcgca acgccgctcg cgtgctccgc
     1981 gcgttctcga gcgccgacca ggatctcggt gtcaccgagc tggccgcgcg cctcggcctc
     2041 gcgctgtacg agctcggcac caacgtcacc gagcacgtcg acctgcacca ggctgcgctg
     2101 ccggttctca cgacgttgcg ccacaacacc ggcgagatgg tccacgtggc cgtcctcgac
     2161 gggctcgagg tcatctacgt cgagcgcctc gagagccacc acctgctgcc gatcttccgt
     2221 accgtgggcc accgcctccc
//
LOCUS       DATCCH010000224         3315 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_563717, whole genome shotgun sequence.
ACCESSION   DATCCH010000224 DATCCH010000000
VERSION     DATCCH010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563717"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1330
                     /locus_tag="VIJ44_04400"
     CDS             <1..1330
                     /locus_tag="VIJ44_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165175.1"
                     /translation="CFATSSPCNDDPNHTTPTLNSPAVVDGDLIATDALGVLVHDTTI
                     VGDVRQSGGGGGATSGPGKNCTPTGIFNKFGSPVFSDYEDSMIGGNLWLTGLQSCWLG
                     ALRDKVGGSVTFSNNIMADPDANEVLTNTIQGNLICAGNSPANQYGDSHGSPDTVGGF
                     ATGQCGFGVLQRNPSIPTGTLEHLALPSNTAAGYDLGAADGGLFSFGTPFFGSAAGTA
                     STMPYVGIATAPGGAGYWLANAGGTVSNFGPNSRSLGSAASFALNKPVVGIAAAPGGD
                     GFWEAAGDGGVFGFGPSASFFGSAGGIHLNRPIVGMAAAPTGDGYDLVASDGGVFTYG
                     PGARFHGSTGSIHLNQPIVGMATDPATGGYWLVAADGGVFSFDAPFLGSLGAIHLNKP
                     IVGIAAAPTGDGYYLVASDGGVFAFGPGAHFQGSTGSIHLNSPIDGIGLG"
     gene            complement(1371..1973)
                     /locus_tag="VIJ44_04405"
     CDS             complement(1371..1973)
                     /locus_tag="VIJ44_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165176.1"
                     /translation="MRTRYVLTLSAVVVLSLITGAMPARASSGPSLAVTPDVGLVEGQ
                     TVAIRGTGFQPSQFVAVVICPTAQLGVFPPDSLEAALYLSTVCSFANNTTSTTAHPDG
                     TFAATFTVTRDNTTTSPGPPFPPAGPPFRCGLAPSDCVILAGSVVGRFVEASAPISFT
                     PPVPISTDECRHDGWRHRADDRGRPFRNQGQCVRFVVHHR"
     gene            complement(2141..2308)
                     /locus_tag="VIJ44_04410"
     CDS             complement(2141..2308)
                     /locus_tag="VIJ44_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165177.1"
                     /translation="MTDHEKLLVALLSESEETNAVHNGRATTLEVMSALYGIGPTPRV
                     PGPVGPTTGAV"
     gene            2442..>3315
                     /locus_tag="VIJ44_04415"
     CDS             2442..>3315
                     /locus_tag="VIJ44_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013018478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
                     /protein_id="HEY5165178.1"
                     /translation="MPVRRIETSPLSDDELARLDAYWRAANYLTVGQIYLLANARLQR
                     PLTLSDVKPRLLGHWGTSPGLNLLYAHLDRVIVRDDLDMIYVIGPGHGGPAILANAWL
                     EGSYTDTYPAITRDAEGMERLFRQFSFPGGVPSHVAPETPGSIHEGGELGYSLAHAFG
                     AAFDNPDLIVACVVGDGEAETGPLATSWHSNKFLDPVGDGAVLPILHLNGYKIANPTV
                     LARISEADLTDLLSGCGWEPFLVSGDDPARVHQELAATLDYVLGEIRAIQDRARTGGE
                     HTRPRWPMIVLRTPK"
BASE COUNT          509 a         1142 c         1133 g          531 t
ORIGIN      
        1 ctgcttcgcc acctcgtcgc cgtgcaacga cgacccgaac cacaccactc cgaccctcaa
       61 cagcccggcc gtcgtggacg gtgacctgat cgcgaccgat gccctcggcg tgttggtaca
      121 cgacaccacg atcgtcggcg acgtccgtca gtcgggcggc ggtggcggag cgaccagcgg
      181 tccgggcaag aactgcacgc cgaccgggat cttcaacaag ttcggctcgc ccgtcttcag
      241 cgactacgag gacagcatga tcggcggcaa cctgtggctc accgggctcc agagctgttg
      301 gctgggcgcc ttgcgcgaca aggtcggcgg cagcgttacc ttctcgaaca acatcatggc
      361 cgaccccgac gccaacgagg tcctcacgaa caccatccag ggcaacctga tctgcgccgg
      421 gaactcaccg gcgaaccagt acggcgactc gcacggcagt cccgacaccg ttggcggctt
      481 cgccaccggg cagtgcggct tcggagttct ccaacgcaac ccgtcgatac ccaccggcac
      541 actcgagcat ctcgccctcc cgagcaatac cgcggcgggc tacgacttgg gcgcggcgga
      601 cggcggcctg ttcagcttcg gcacaccctt cttcgggtcg gcggccggca ccgcgtcgac
      661 aatgccgtac gtcgggatcg ccaccgctcc gggcggcgcc ggctactggc tggccaacgc
      721 gggcggaacc gtgagcaact tcggacccaa cagccggtcg ctcgggtcgg ccgcctcgtt
      781 cgccctgaac aagccggtgg tcgggatcgc agcggcgccc ggaggcgacg gcttctggga
      841 ggcggccggc gacggcggcg tgttcggctt cgggccgagt gcgtcgttct tcgggtccgc
      901 cgggggcatt catctcaacc ggccgatcgt cggcatggcc gcggctccta ccggtgacgg
      961 ctacgacctc gtcgcctccg acggtggcgt gttcacgtac gggccgggcg cccggttcca
     1021 cggttccacc ggcagcatcc atctcaacca gccgatcgtc ggcatggcga cggacccggc
     1081 gaccggcggc tactggctcg tcgcggccga cggtggggtg ttcagcttcg acgctccgtt
     1141 cctcggctcg ctcggtgcca tccacctcaa caagccgatc gtgggaatcg ccgccgctcc
     1201 gacgggtgac ggctactacc tcgtcgcctc cgacggcggt gtcttcgcat tcgggcccgg
     1261 agcgcacttc cagggttcga ccggaagcat tcatctgaac tcgccgatcg acggaatcgg
     1321 cctcggctga cctcgggatc aaggacggcg tcgcttcggt ggtgcactac tcatcggtgg
     1381 tgcaccacga agcgtacgca ctgaccctgg ttgcggaagg gccgaccgcg gtcgtcggcg
     1441 cgatgacgcc agccgtcgtg ccggcactcg tcggtgctga tcggaacggg tggtgtgaac
     1501 gagatcggtg ccgacgcttc gacgaagcgt ccgacgacac tgccggcgag gatcacacag
     1561 tccgagggtg cgagcccgca gcggaacggc gggcccgcgg gcgggaaggg cggccccgga
     1621 ctcgtggtcg tgttgtcgcg ggtcacggtg aatgtcgcgg cgaacgtccc gtccgggtgc
     1681 gcagtggtcg aggtggtgtt gttcgcgaac gagcacacgg tcgacaggta gagggcggcc
     1741 tcgagcgagt ccggtgggaa cacgcccaac tgcgcggtcg gacagatcac gacggccacg
     1801 aactggctcg gttggaagcc cgttcctctt atcgcgaccg tctgtccctc caccagcccg
     1861 acgtcgggtg tgactgcgag tgacgggccg ctcgacgccc gggcgggcat cgcgccggtg
     1921 atcagcgaga gcaccacgac cgcagagaga gtcaagacgt acctggtgcg cacgggctcc
     1981 ctcctcgtca gttgcgcgcc ggtatagccc gtcgagaact gggcgtcgcg cccgaacacg
     2041 ggatcgcgga ggtggaccga cccggtctgg ctcccttcgg ccgcgacgtg ttcgacactt
     2101 cgccgcaccg ccaatggccg ccccgccggc acgggcgggg ctagacggcc ccggtcgtcg
     2161 ggccaaccgg tccgggaacc cgaggcgtcg gtccgatgcc gtagagcgcg gacatcacct
     2221 cgagcgtcgt cgctcgaccg ttgtggacgg cgttggtctc ctcggactcg ctcaacaacg
     2281 cgacgaggag cttctcgtgg tcggtcatgt cactcctttt tccgtgtgtc tgaccgaggc
     2341 cttccggcgt atctgaccga ggcggcgtga cggctgcgtg acgtcacggc tgcgtgacgc
     2401 cgcggatgcg cgggtgccag gtgcgcgcat cgggtagacc tgtgccggtg cgccggatcg
     2461 agacgtctcc gctctccgat gacgagttgg cgcgcctcga cgcgtactgg cgagccgcga
     2521 actacctcac ggtcggccag atctacctgc tcgcgaacgc gcggttgcag cgaccgctga
     2581 cgttgtccga cgtcaaaccg cggctgttgg gtcactgggg gacgagcccg ggactgaacc
     2641 tcctgtacgc gcacctcgac cgcgtgatcg tgcgcgacga cctcgacatg atctatgtga
     2701 tcggtcccgg tcacggtgga cccgcgatcc tcgcgaacgc gtggctcgag ggcagctaca
     2761 ccgacaccta tcccgcgata acgcgcgacg ccgaggggat ggagcggctg ttccggcagt
     2821 tctcgttccc gggtggcgtg ccgagccacg tggcacccga gacgccgggt tccatccacg
     2881 agggcgggga gctcggttac tcgctcgcgc acgcgttcgg cgccgccttc gacaatcccg
     2941 atctgatcgt cgcgtgcgtg gtaggtgatg gcgaggcgga gacgggaccg ctcgcgacga
     3001 gttggcactc gaacaagttc ctcgacccgg tcggcgacgg cgcggtgctc ccgatcctgc
     3061 acctcaacgg ttacaagatc gcgaacccga cggtgctggc gcgcatctcc gaggctgacc
     3121 tcaccgacct cctgtccggc tgcggctggg agccgttcct ggtatccgga gacgatccgg
     3181 cgcgcgtgca tcaggagctc gcggcgaccc tcgactacgt gctcggtgag atacgggcga
     3241 tccaagaccg cgcccgcacc ggcggtgagc acacccggcc gcgctggccg atgatcgtgt
     3301 tgcgaacccc gaagg
//
LOCUS       DATCCH010000225         5011 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_522815, whole genome shotgun sequence.
ACCESSION   DATCCH010000225 DATCCH010000000
VERSION     DATCCH010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5011
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522815"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="VIJ44_04420"
     CDS             complement(<1..368)
                     /locus_tag="VIJ44_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY5165179.1"
                     /translation="MTSAPTRRAAGHPNVDGALRPLLASVRSSDAQRDTALIEDAYEV
                     ARDAHRDQVRRSGDPYIAHPLGVAMILSDLGLDDVTLAGALLHDAVEDTSVTLDDLEA
                     RFGHDVAAIVDGVTKLDRLE"
     gene            complement(361..885)
                     /locus_tag="VIJ44_04425"
     CDS             complement(361..885)
                     /locus_tag="VIJ44_04425"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEY5165180.1"
                     /translation="MDPAWLKAHVRDVLDYPKPGIVFKDITPLLGAADAFRAAVDALA
                     EPFAATRVDKVIGIEARGFVFAAPVSYGLLAGFVPVRKTGKLPWEIEREEYALEYGTD
                     LLEIHRDAIAPGEQVLVVDDVIATGGTAAATARLVERLGGEVVGFAFVLELGFLHGRD
                     QLDGYQVHTLVTYD"
     gene            complement(958..2403)
                     /gene="secF"
                     /locus_tag="VIJ44_04430"
     CDS             complement(958..2403)
                     /gene="secF"
                     /locus_tag="VIJ44_04430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEY5165181.1"
                     /translation="MTITAAPPPEIHDVPGVTRRHRPSDLYHERTNFQFIKHSRRWLI
                     ISSTLVVLSLLLLSVRGLNLGIDFEGGTAWQVQMKGARSAKVAEVRSLLGTLGFDNAN
                     VSVLTPPGGGAPSIRVEARVIDDPTHVVQVALAKYGGVTDADVAFSQSGSGGSFTFTA
                     KAGVNATGDGVKSALVGTRLTNPQVKVSGRTVTVTVTTLPPSQVQSVASALAKYAGAT
                     VNDVSITTVGPTWGREVSDKALKALIIFFFVLAAYLAIRFEWKMSAAAIVAVIHDIIF
                     TVGVYALFHFQVSPATVTAFLTILGFSLYDTVVVFDKVVEFQRTLTATGRSTYGEMVN
                     RSLNAVLMRSVSTSLVALLPVVSLLVVGSGILGATALEDFALALAAGLFIGSYSSIFV
                     AAPLLAWWKEREPQYRALAERRRRQPEAAGPGAVTVAASAPSNLATPGAAPVEPGEAV
                     ERKPLGIPGPPAVPRTTIQARPRQQRGKKRR"
     gene            complement(2400..4079)
                     /gene="secD"
                     /locus_tag="VIJ44_04435"
     CDS             complement(2400..4079)
                     /gene="secD"
                     /locus_tag="VIJ44_04435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEY5165182.1"
                     /translation="MRRKVILLLVTVVLILGAFVATLFGSSRPVLGLDLQGGTSIVLA
                     PVKGSDLSTLDTAVAIIRNRVDGLGIAEPNVSRQGDNIVIDLPGVKNAAEADALVGKT
                     AELRFRLVQGTIPYSSTAASGQTTTTVKGATTTTVKGATTTTVKAATTTTLKGATTTT
                     TAKAATSTTAHARGRTGTIDTVPIAASRPEAVTSSTATATTVKGATATTVKGATTTTV
                     KGATTTTVPAADAGTCQNGKIVTPDVADKSVVLPDLNHTACYLLGPTILTGRGVGTAS
                     AIIDTTTAAWIVNVHFKNNDFVTKVAVPYVTKQVAIELDGVVQSAPTINQGITGQDVT
                     ISGTFTSKQAHQLALVLRYGSLPVQLVSQTAQSVSPTLGKDQLRAGIVSGLIGLALVA
                     LYMIFFYRLLGLVVWTGIGLTALTFFALVSWLSSHQGLTLTLAGVTGIIVSVGVTVDS
                     YVVYFERLKDEVRTGKTVRSSLDSGFRRAFRTIIAADLVSMIGAVVLYLFATSSVRGF
                     AYFLGLSTALDLILAYCFMWPFVSVLARQPALVRMGGVGIGAGLDVPEVSA"
     gene            complement(4083..4379)
                     /gene="yajC"
                     /locus_tag="VIJ44_04440"
     CDS             complement(4083..4379)
                     /gene="yajC"
                     /locus_tag="VIJ44_04440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEY5165183.1"
                     /translation="MAIFPVLMVGAIYLLLWRPQQRRIAAVRRLQNEIQEGDDVLTTS
                     GIYGRITKLGESEADLEIAPGTVIHIARGAIGQRIGPESLPQAGDPHDGETGAD"
     gene            complement(4447..>5011)
                     /locus_tag="VIJ44_04445"
     CDS             complement(4447..>5011)
                     /locus_tag="VIJ44_04445"
                     /inference="COORDINATES: protein motif:HMM:NF004858.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEY5165184.1"
                     /translation="LLISGAPGMFSGGFDLQVMRGGPAAAGKLVTDGGELFARMYGSA
                     LPVVVACTGHAIAAGALLLLAADIRVGARGEFRIGLIETQLGMVLPRWAVEFAHERLS
                     KRHVQDATVGARIYDPDGAADAGFLDAVVEPAALAEAATAEAQRWAALSRDAYRGQVR
                     VCRGDRLGRLAEAIAADRGRVFEMPGS"
BASE COUNT          814 a         1700 c         1755 g          742 t
ORIGIN      
        1 aactcgagcc gatcgagctt cgtgacaccg tcgacgatcg cggcgacatc gtggccgaat
       61 cgcgcttcga ggtcgtcgag cgtcaccgac gtgtcctcga cggcgtcgtg cagcagcgcg
      121 ccggcaagcg tgacgtcgtc gagaccgaga tcggagagga tcatcgccac gccgagcggg
      181 tgggcgatat acggatcgcc ggatcggcgc acctggtcgc ggtgtgcatc ccgcgcgacc
      241 tcgtacgcgt cctcgatgag cgcggtgtcg cgctgcgcgt cgctactgcg caccgacgcg
      301 aggagcgggc gcagggcacc gtcgacgttc gggtgaccgg cagctcgccg ggtcggcgca
      361 ctagtcatac gtcaccagcg tgtgcacttg gtagccgtcg agctggtcgc ggccgtgcag
      421 gaatccgagc tcgagcacga acgcgaagcc gacgacctcg ccgcccaggc gttcgaccaa
      481 ccgggcggtc gccgcggccg taccaccggt cgcgatcaca tcgtcgacca cgagcacctg
      541 ctcacccggc gctatcgcat cgcggtggat ctcgagcaaa tcggtgccgt actcgagcgc
      601 gtactcttcg cgctcgatct cccagggcag cttgccggtc ttgcggacgg gcacgaaccc
      661 ggcgagcagg ccgtacgaca cgggggcggc gaaaacgaag cctcgagctt cgatcccgat
      721 caccttgtcg acgcgcgtcg ccgcgaacgg ctccgcgagc gcgtcgaccg ccgcccggaa
      781 cgcgtccgcg gccccgagca gcggggtgat gtccttgaag acgatccccg gcttcgggta
      841 gtcgaggaca tcgcgcacgt gtgccttcag ccaggctgga tccatccata ccagcgtacc
      901 gacggacgcg cgagcgaacg cgccccggcc cgccgtgcgg tgcgtcggac ccggccgcta
      961 ccggcgcttc ttcccgcgct gctggcgggg gcgcgcctgg atggtggtgc gggggacggc
     1021 gggtggcccg gggattccga gcggcttgcg ctcgaccgcc tcgcccggct cgacgggcgc
     1081 ggccccggga gtcgcgaggt tcgacggagc cgacgcggcc accgtgaccg cgccggggcc
     1141 cgcggcctcg ggctgccgtc ggcgccgctc ggcgagcgcc cggtactggg gctcacgctc
     1201 cttccaccac gccaacaacg gtgcggcgac gaagatcgac gagtacgagc cgatgaacag
     1261 gccggccgcg agcgcgagcg cgaagtcctc gagcgcagtc gcgccgagaa tgccgctgcc
     1321 gacgacgagt aacgagacga ccggcaacag tgcgacgagc gacgtcgaca ccgaccgcat
     1381 cagcaccgcg ttgagcgacc ggttcaccat ctcgccgtag gtcgaccgtc ccgtcgcggt
     1441 gagcgtgcgc tggaattcga cgaccttgtc gaacaccacg acggtgtcgt acaaggagaa
     1501 cccgaggatc gtcaggaacg cggtgacggt cgccggcgag acctggaagt ggaacagcgc
     1561 gtacacgccg accgtgaaga tgatgtcgtg gatgacggca acgatcgcgg cggccgacat
     1621 cttccactcg aatcggatcg cgaggtaggc ggccaggacg aagaagaaga tgatcagggc
     1681 cttgagcgcc ttgtcgctca cctcgcgccc ccaggtggga ccgaccgtgg tgatactgac
     1741 atcgttgacg gtggcacccg cgtatttggc cagtgccgac gcgaccgatt gcacctggct
     1801 gggcggcagg gtcgtgaccg tgaccgtgac cgtgcgcccg ctcaccttga cttgcgggtt
     1861 ggtgagccgc gtgccgacga gcgcgctctt cacgccgtcg ccggtggcgt tcactcccgc
     1921 cttcgcggtg aacgtgaacg agccccccga gccgctctgc gaaaacgcca cgtcggcgtc
     1981 ggtgacgccg ccgtacttgg ccagcgccac ctggacgacg tgggtcgggt cgtcgatgac
     2041 gcgtgcctcg actcggatgc tcggcgcgcc gccacccggc ggcgtcagca cgctgacgtt
     2101 cgcgttgtcg aagccgagcg tcccgagcag gctccgtacc tcggcgacct tggcgctcct
     2161 cgcgcccttc atctgcacct gccaggcggt tccaccctcg aaatcgatcc cgaggttgag
     2221 cccgcgcacg gacaagagca gcaagctcag cacgaccaga gtgctcgaga tgatcagcca
     2281 gcggcggctg tgcttgatga actggaagtt ggtccgctcg tggtagaggt cggacggccg
     2341 gtgcctccgg gtgacgccgg ggacgtcgtg gatctccggc gggggcgcgg ccgtgatcgt
     2401 cacgcgctca cctcgggaac gtcgaggccg gcgccgatac cgacgccacc catgcgaacg
     2461 agcgcgggtt gacgggcgag cacggacacg aacggccaca tgaagcagta ggccaggatc
     2521 aagtcgagcg ccgtcgagag cccgaggaag tacgcgaacc cgcgcacgga agaggtcgcg
     2581 aagagataca ggaccaccgc gccgatcatg gacacgaggt cggcggcgat gatggtgcgg
     2641 aacgcccgcc ggaagccgga gtcgagcgag gagcgcacgg tcttaccggt gcgcacctcg
     2701 tccttcaatc gctcgaagta gacgacataa ctgtcgacgg tcacaccgac cgagacgatg
     2761 atgcccgtca cccccgcgag cgtgagggtc agaccctggt gcgacgagag ccacgacacg
     2821 agcgcgaaga acgtgagtgc ggtgagcccg atgcccgtcc atacgacgag gccgagcagc
     2881 cggtagaaga agatcatgta gagcgcgacg agtgcgaggc cgatcagacc cgacacgatg
     2941 ccggcccgca gctgatcctt gccgagcgtc ggcgacaccg actgcgcggt ctgggagacg
     3001 agctggacgg gcagcgagcc gtagcgcagc acgagcgcga gctggtgcgc ctgcttgctc
     3061 gtgaaggtac cgctgatcgt gacgtcctga ccggtaatcc cctggttgat cgtcggcgcc
     3121 gactgcacga cgccgtccag ctcgatcgcc acctgcttgg tcacgtacgg caccgcgacc
     3181 ttcgtgacga agtcgttgtt cttgaagtgc acgttgacga tccacgccgc ggtcgtcgtg
     3241 tcgataatgg cgctggcggt accgactcct cggccggtga ggatggtcgg gccgaggagg
     3301 taacacgcag tgtggttcag gtcgggaagc acgaccgact tgtcggcgac gtccggcgtg
     3361 acgatcttgc cgttctgaca ggtacccgcg tcggccgccg gcaccgtggt ggtggtcgcg
     3421 cccttcaccg tcgtcgtggt cgcgcccttt accgtcgtcg cggtcgcgcc cttcaccgtc
     3481 gtggcggtcg cggtcgagga tgtcaccgct tccggacgcg acgccgcgat cgggaccgtg
     3541 tcgatcgtgc cggtgcggcc ccgcgcgtgc gcggtcgtgg aggtggcagc tttggccgtg
     3601 gtggtggtgg tcgcgccctt caacgtcgtc gtggtcgcgg ccttcaccgt cgtcgtggtc
     3661 gcgcccttga ccgtcgtcgt ggtcgcaccc ttgacggtgg tggtggtttg ccccgaagcg
     3721 gccgtgctcg agtagggaat ggtcccttga accagacgga accgcagttc ggccgtcttg
     3781 ccgacgaggg cgtcggcctc ggccgcgttc ttcacgcccg ggaggtcgat gacgatgttg
     3841 tcgccctgac ggctgacgtt cggttcggcg atcccgagac catcgacgcg gttgcggatg
     3901 atcgcgaccg ccgtgtcgag cgtcgagagg tcggagccct tcaccggggc caggacgatg
     3961 gacgtgccgc cctggaggtc gagaccgagg acgggacggc tcgaaccgaa gagggtggcg
     4021 acgaaggcac cgaggatcag cacgaccgtc accaggagca ggatgacctt gcggcgcacg
     4081 gttcagtctg ccccggtctc accgtcgtgg gggtcaccgg cctgtggcag cgactccggc
     4141 ccgatgcgct gtccgatcgc gccgcgcgcg atgtggatca cggtgccggg cgcgatctcg
     4201 agatcggcct cggactcacc gagcttggtg atccttccgt agatcccgct ggtggtcagc
     4261 acgtcgtcgc cctcttgaat ctcgttctgc agccgccgta ctgcggcgat ccgccgctgc
     4321 tgggggcgcc acagcaacag gtagatggcg cccaccatga gcacggggaa gatcgccacc
     4381 acgagagagc cctttacgcc ggagaaacgg gaaccgaagc taccaccgcc ggtcgccggt
     4441 cccgggtcac gatccgggca tttcgaagac ccggccccga tcggcggcga tcgcctcggc
     4501 caaccggccg agccggtcac ctcggcacac gcggacctgg cctcggtagg cgtcccgcga
     4561 gagcgcggcc cagcgctgcg cctcggcggt tgcggcctcg gccagcgccg ccggctccac
     4621 gaccgcatcg aggaaacccg cgtcggccgc gccgtccggg tcgtagatgc gggcgccgac
     4681 ggtcgcgtcc tgcacgtgac gcttcgacag tcgctcgtgc gcgaactcga cggcccagcg
     4741 cgggagcacc attccgagct gggtctcgat gagcccgatg cggaactcgc cccgcgcccc
     4801 gacccgaatg tccgccgcga gcaacagcag tgcgcccgcc gcgatcgcgt gacccgtgca
     4861 cgccacgacc accgggagcg cggagccgta catacgcgcg aagagctcgc cgccgtcggt
     4921 gacgagcttt cccgccgcgg ccggaccgcc gcgcatgact tggagatcga acccgccgga
     4981 gaacatgccc ggcgcacccg agatcaacag c
//
LOCUS       DATCCH010000226         2586 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_314441, whole genome shotgun sequence.
ACCESSION   DATCCH010000226 DATCCH010000000
VERSION     DATCCH010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314441"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /locus_tag="VIJ44_04450"
     CDS             <1..859
                     /locus_tag="VIJ44_04450"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEY5165185.1"
                     /translation="VVDVVLAGGVESMSRVPLGAAVSAGPGRPLPKAYFDQFEFSSQF
                     ESADRIADKWGITREDCDRFGLRSQQLAKTAWAEGRFEREVVPIVAPDPGDDGKPAAT
                     THRVERDEGLRETSLEALASLKPVGRDDGVHTAGTSSQITDGAAAVLLTSAARAEALG
                     LRPRARIVDQCLVGVDPVLMLTGPIDATRRLFDRTGLAMRDLDTIEINEAFASVVLAW
                     AQEVQPDLDIVNPNGGAIAIGHPVGATGARLVTTALHELERIDGRYGLVTMCCGGGLG
                     TGTIIERLA"
     gene            870..1721
                     /locus_tag="VIJ44_04455"
     CDS             870..1721
                     /locus_tag="VIJ44_04455"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY5165186.1"
                     /translation="MTQLAPRSPLAERLGYPPDARLVIVNCDDLGSSRSANVGVYDAL
                     RNGLGTSASLMVPCPWARDAAAQYRGEDVGVHLTLNAEWETYRWAPITNSPSLLDGDG
                     GFPRTVEDTWDHADLEEVRRELRAQIERAILWGFDVSHLDSHMGTLQLRADFFGVYLE
                     LAVDFGLPLRMGPMGGERVVGFPYREVASAEGVVYPDHFVLYRGAGARRHVERALFDL
                     RPGVTEVYLHPAVDADELRASHPDWAGRVEDHAFLCEDPSLPDLLERAGATLIGFREL
                     RALQRAA"
     gene            complement(1730..2116)
                     /locus_tag="VIJ44_04460"
     CDS             complement(1730..2116)
                     /locus_tag="VIJ44_04460"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEY5165187.1"
                     /translation="MPKYLSQEWLDEARALAQDQPERPGASATMQFVVTGGPDGDIKY
                     YWVLENGQLAEASLGELPDAEVTLTTGYADSVAIQKGDVDANAAFMQGKTKVTGNMAK
                     LMLLMPLTMSPEYRALQDQVQARTEY"
     gene            complement(2139..>2586)
                     /locus_tag="VIJ44_04465"
     CDS             complement(2139..>2586)
                     /locus_tag="VIJ44_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="mycothiol conjugate amidase Mca"
                     /protein_id="HEY5165188.1"
                     /translation="EAAGDPERYPDAGTAWQPSKLYYTGFSRRRVEALHAAHLARGDE
                     SPFERWFERGFPRNEHRFTTFVEIGPWLKRRRAALLAHRTQVDPKGFWMRLPDDVIRE
                     VFPWDEFVLARSLVGRPADGDTEDDLFAGVRASDARDRVDTLPRRS"
BASE COUNT          370 a          885 c          912 g          419 t
ORIGIN      
        1 tgtcgtcgac gtcgtcctgg ccggcggggt cgagtcgatg agccgggtgc cgctcggcgc
       61 cgcggtgtcg gcggggccgg gacggccgct cccgaaggcg tacttcgacc agttcgagtt
      121 ctcgtcgcag ttcgaaagcg ccgaccgcat cgccgacaag tggggcatca cccgcgagga
      181 ctgtgaccgc ttcggattgc ggtcccaaca gctggcgaag accgcgtggg ccgaaggccg
      241 gttcgagcgt gaggtcgttc ccatcgtggc gcccgacccc ggcgacgacg gcaaaccggc
      301 cgcgacgacc catcgggtcg aacgcgacga gggcctgcgc gagacgtcgc tggaggcgct
      361 ggcgtcactg aaacccgtcg gccgggacga cggcgtccac acggccggaa cctcctcgca
      421 gatcaccgac ggggccgcgg ccgtcctgtt gacgtccgcg gcgcgcgccg aggcgctcgg
      481 cctgcgaccc cgagcgcgga tcgtcgatca gtgtctggtc ggggtcgacc cggttctcat
      541 gctcaccggg cccatcgacg ccactcgccg cttgttcgat cgcaccggac tcgccatgcg
      601 cgacctcgac acgatcgaga tcaacgaggc gttcgcgtcg gtggtgctgg cctgggccca
      661 ggaggtccaa cccgacctgg acatcgtgaa ccccaacggt ggcgcgatcg ctatcggtca
      721 cccggtcggg gccaccggcg cccgcctggt gaccaccgcg ctgcacgaac tcgagcgcat
      781 cgatgggcgc tacggcctcg tcaccatgtg ctgcgggggc gggctcggta ccgggacgat
      841 catcgagcgg ctcgcgtagc ctttggcgcg tgacgcagct ggcgcccaga tccccactcg
      901 ctgagcggtt ggggtaccca cctgacgctc ggctcgtcat cgtcaactgt gacgacctcg
      961 gctcgtcgcg atccgcgaac gtcggggtct acgacgcgct ccgcaacggg ctgggcacca
     1021 gcgcgtcgct gatggtcccg tgcccgtggg cccgcgacgc cgcggcgcag taccggggtg
     1081 aggacgtcgg ggtgcacctc acattgaacg ccgaatggga gacgtaccgg tgggcgccga
     1141 tcacgaacag cccgagcctg ctcgacgggg acggtggctt cccgcgcacg gtcgaggaca
     1201 cttgggatca cgcggatctc gaagaggtcc gccgggagct gcgggcccag atcgaacggg
     1261 cgatcctctg ggggttcgac gtcagtcatc tcgacagcca catgggcacc ctgcagctgc
     1321 gcgccgactt cttcggtgtg tacctggagc tggcggtcga cttcgggctc ccgttgcgga
     1381 tgggcccgat gggaggtgag cgggtcgtcg gattcccgta ccgggaggtc gcctctgccg
     1441 aaggtgtcgt ctacccggat catttcgtgc tgtatcgcgg ggctggcgcc cgtcggcacg
     1501 tggagcgggc cctgttcgac ctgcgacccg gggtcaccga ggtgtacctg caccccgcgg
     1561 tcgatgccga cgagctgcgc gcctcacatc cggactgggc cggccgggtc gaggaccatg
     1621 ccttcttgtg cgaggacccg tccctgccgg acctgttgga acgtgccggc gccacgctga
     1681 tcgggtttcg cgagctccga gcgctccagc gcgcggcgta acgccgcgat cagtactcgg
     1741 tgcgggcctg cacctgatcc tggagggcgc ggtactcggg tgacatggtc agcggcatga
     1801 gcagcatgag cttcgccatg ttgcccgtca ccttggtctt gccttgcatg aaggcggcgt
     1861 tggcgtccac gtcgcctttc tggatcgcga ccgagtccgc atagccggtg gtgagtgtga
     1921 cctcggcgtc ggggagctcg cccagtgacg cttcggcgag ctggccgttc tcgaggaccc
     1981 agtagtactt gatgtcgccg tcgggcccac cggtcacgac gaactgcatc gtggcgctgg
     2041 cccccggacg ctcaggctga tcctgggcca gcgcgcgcgc ctcgtcgagc cattcctggc
     2101 tgaggtattt cggcacgacc cttcccctcg cggtcccgtc acgaacgacg ggggagtgta
     2161 tcgacgcggt cgcgggcgtc ggaggcccgc accccggcga acaaatcgtc ctcggtgtcg
     2221 ccgtcagccg gacgtcccac gagggaccgc gccagcacga actcgtccca ggggaacacc
     2281 tcccggatga cgtcgtcggg gaggcgcatc cagaaccctt tcgggtcgac ctgcgtccga
     2341 tgcgcgagca gcgcggcccg tcgtcgcttc aaccacggac cgatctcgac gaacgtcgtg
     2401 aaccggtgct cattccgagg gaagccccgc tcgaaccagc gttcaaaggg gctctcgtca
     2461 ccgcgggcca ggtgcgcggc gtggagggcc tcgacgcgtc gacgcgagaa ccccgtgtag
     2521 tagagcttcg atggctgcca cgcggttccc gcgtcgggat agcgctcggg gtcgccggcc
     2581 gcctcg
//
LOCUS       DATCCH010000227         2095 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_219711, whole genome shotgun sequence.
ACCESSION   DATCCH010000227 DATCCH010000000
VERSION     DATCCH010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219711"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..853
                     /locus_tag="VIJ44_04470"
     CDS             <1..853
                     /locus_tag="VIJ44_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HEY5165189.1"
                     /translation="RLVSFAQQHDQLVRGHTLVWHNQLPDWLTSGVSNGTISNTDLRN
                     LLHKHITDEVTHFRGQIWQWDVANEFFTDSNPSGINPNDFWISHLGTGVLADAFRWAH
                     AADPHSLLFFNDYNIAGEDGSNAKSDAVYAWVKTMLAQHVPIDGIGDQGHLDTQYGFP
                     TQMTADLQRYAKLGLKVAITEADVRTFVNNATDQVPTDHLATFAQPYEFTKMLKACLA
                     VQSCISFTVWGFGDANSWIPGFFTGEGYATIYDVNLNPKPAYLDMQEDLTLAARGAPR
                     RGFGDEH"
     gene            1317..>2095
                     /locus_tag="VIJ44_04475"
     CDS             1317..>2095
                     /locus_tag="VIJ44_04475"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5165190.1"
                     /translation="MGGVEQRLALRALTSGRVSTSSPDEPALVVEHLTKRFGERVAVD
                     DVSFSVAAGEVFGFLGPNGAGKTTTVRILATLIAPTSGTARVAGLLLDPANGVDIRKR
                     IAVMTENPGLYLRLTVAENLEFFAGLYELVEPKERIGQALAAVNLAGRANDPCGSLSK
                     GLRQRVALARTLLSDPAVVFLDEPTSGLDPVASREVHELIDGLRERGVTVFLTTHRLE
                     EAERLCDRVAILNTSMRTIGRPDEFRDRLFTKSLVVRTVAPL"
BASE COUNT          341 a          756 c          655 g          343 t
ORIGIN      
        1 ccggctcgtc agcttcgccc agcaacacga ccagctcgtg cgtgggcaca ccctcgtctg
       61 gcacaaccag ctccccgact ggctgaccag cggcgtgagc aacggaacga tcagcaacac
      121 cgacctgcgc aacctgttgc acaagcacat caccgacgag gtcacccact tcagaggcca
      181 gatctggcag tgggacgtcg ccaacgagtt cttcaccgac agcaatccgt cgggcatcaa
      241 cccgaacgac ttctggatct cccacctcgg cacgggcgtt ctcgccgacg ccttccggtg
      301 ggctcacgca gccgacccgc actcgctgct cttcttcaac gactacaaca tcgccggcga
      361 ggacggatca aacgccaaga gcgacgcggt ctacgcgtgg gtgaagacga tgctcgccca
      421 acacgtaccg atcgacggga tcggcgacca gggacacctc gacacccagt acgggttccc
      481 gacccagatg actgcagacc tgcagcgcta cgccaagctg gggctcaagg tcgccatcac
      541 cgaggccgac gttcgcacct tcgtcaacaa cgccaccgac caggtaccga ccgaccacct
      601 ggccaccttc gcgcagccct atgagttcac gaagatgctc aaggcctgtc tcgccgtcca
      661 gagctgcatc tcgttcaccg tctggggctt tggcgacgcg aactcgtgga tccccggctt
      721 cttcacaggg gagggatacg cgaccatcta cgacgtcaac ctcaacccca agcccgccta
      781 cctggacatg caggaggatc tgaccctcgc ggctcgcggc gcgccccggc gtggctttgg
      841 agacgagcac tagcactagc taccgcgacg catacctcgc cgaggcgggc cgctcgtgcg
      901 gtccgcctcg gccccgaacc tctgacggat cagtctcatc atctgttcga gccttatgac
      961 ggtgagagcg ccgttgccgt cgtcgcgtcc cggcgccacc gctaggctcc cggtcgtcgc
     1021 cgagttgagg ttgtccgagt gaggcttcgc gtctgatggg caggccatgg ttccgccggc
     1081 gccaggtcgg cttgggttgg cgaccgatca ggtggcaggg ctggctcgtc accgtcggcg
     1141 ccgtcgccct cgccgtcggc gcgctgacgc tcctgcacgc ctcatccgcg cgcgttccga
     1201 tcgtcatcct gatcgttgct gtctacgccg ttgtcgcggt cgcgaccggt ggtgccaggt
     1261 ctgcagaggc cgctgctccg ggggatcagc cgaccgatgg cgaggccgag atcggcgtgg
     1321 gcggcgtcga gcagcggctg gcgctccggg cgctaacgtc cgggcgggta tcgacgagtt
     1381 cgccggacga gccggcgctg gtcgtcgagc atctgacgaa gcggttcggg gagcgggtcg
     1441 cggtcgatga tgtctccttc agtgtcgccg ccggtgaggt gttcggcttt ctcggcccga
     1501 acggcgccgg caagaccacc accgtgcgca tcctcgccac cctgatcgcc cccacctcgg
     1561 gcacagcccg cgtggcgggc ctgctgctcg acccggcaaa cggcgtcgac atccgcaagc
     1621 ggatcgcggt gatgaccgag aatcccggcc tctacctgcg tctcaccgtc gccgagaacc
     1681 tcgagttctt cgccgggctg tacgagctcg tagagccgaa ggagcggatc gggcaggcgc
     1741 tggcggcggt caaccttgcc ggccgggcga acgatccctg cggcagcctc tcgaaggggc
     1801 tccgccaacg cgtcgcgctc gcgcggacgc tgctcagcga tccggcggtc gtctttctcg
     1861 acgagccgac ctccggtctc gacccggtcg catcccgcga ggtgcacgaa ctgatcgacg
     1921 gcctgcgcga acgaggcgtt accgtctttc tcaccaccca ccggctcgag gaagccgagc
     1981 ggctctgcga ccgcgtcgcc atcctcaaca ccagcatgcg cacgatcggc cggccggacg
     2041 agtttcgcga ccggctcttc accaagtcgc tcgtcgtgcg caccgtggcg ccgct
//
LOCUS       DATCCH010000228         3699 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_386174, whole genome shotgun sequence.
ACCESSION   DATCCH010000228 DATCCH010000000
VERSION     DATCCH010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3699
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386174"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..430)
                     /gene="rnpB"
                     /locus_tag="VIJ44_04480"
     ncRNA           complement(57..430)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VIJ44_04480"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            534..1604
                     /gene="bioB"
                     /locus_tag="VIJ44_04485"
     CDS             534..1604
                     /gene="bioB"
                     /locus_tag="VIJ44_04485"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEY5165191.1"
                     /translation="MAVPAALARAETQLIDRDEPLTRDEVAAVGALPLDTLPHLVALA
                     HRVRLAYCGPSVELESLINAKSGACAEDCAFCSQSARFHTDVDVYPFLDLDEVMDAAR
                     ATRAAGATQFCIVVAVRNPEERLLRKVIDAAAAVQHDVGLEVACSLGLLTQEQAERLA
                     AAGVRRYNHNLETCRAVFPQICTTHTYDDRVDTARLAIEAGMELCCGGILGLGETLEQ
                     RLDFAFELAALDPCEVPINFLDPRPGTPLADQALLSPREALQAIALFRLVLPRAWLRL
                     AGGRELVLGELQSMGLLAGANALIVGNYLTTTGRGADEDRALLDALGMPVADGPGEGR
                     FVVDADGAHPRPARRPIAVHAE"
     gene            complement(1644..2237)
                     /locus_tag="VIJ44_04490"
     CDS             complement(1644..2237)
                     /locus_tag="VIJ44_04490"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY5165192.1"
                     /translation="MIVLVRHGQTEANRAGRLLGRADPPLNERGRHQADALATVIEHG
                     PAPSLVLTSPLLRARETAERVSAATGTPVRIDERLVELDYGEWDERLLRDIPADVGTR
                     WRSDPSFAAPGGESLVDLRSRVTPCAEGLMELASDGVIIVVSHVSPIKAIICWALDLD
                     DSYAWRLRLDVASISRLVAGLNGPVLLSFNETGHLLG"
     gene            complement(2234..2710)
                     /locus_tag="VIJ44_04495"
     CDS             complement(2234..2710)
                     /locus_tag="VIJ44_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015759640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165193.1"
                     /translation="MLFWYTGLSILIVANVFRSTGLDYRLVAVGALLPVIVDLPAGHR
                     AFGHALLFPVGLFAAVMVVTAGRSRLLRRRLLCVPIGVFVALVLSGAFTQDHVFLWPF
                     LGGAGHLSLLPVWWAVVLEELAGVAAWWWVVGQFDLYLAQPRRDFWHGGRLREGEP"
     gene            complement(2717..3136)
                     /locus_tag="VIJ44_04500"
     CDS             complement(2717..3136)
                     /locus_tag="VIJ44_04500"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF024848.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HEY5165194.1"
                     /translation="MNRHDEVTIFCDGGARGNPGPAAIGAVVTDPTTEPPTQLATVSE
                     RIGVATNNVAEYRAVIAGLQAARPYHARVVHLRADSMLVIRQLEGVWRIKQPHLRPLH
                     AEAKALLDSYAAVDLAHVRREQNVDADLLVNAALDQS"
     gene            complement(3133..>3699)
                     /locus_tag="VIJ44_04505"
     CDS             complement(3133..>3699)
                     /locus_tag="VIJ44_04505"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HEY5165195.1"
                     /translation="QQAERDDVAGGEQRLADEAQRLGEQAAAAEQRLYSGEVSSPREL
                     QALQADVEQLRRHQREVEDRQLSAMEQREPLDATVGALDADGASLESEAAVLQRDLEA
                     RERELDEAAAVERAARDELAGAIDAGLVSEFERRRERGNGVGAARLSGMTCQGCHLSI
                     PATEVDRIRKQGDGALAFCDNCGCILVP"
BASE COUNT          533 a         1363 c         1268 g          535 t
ORIGIN      
        1 tccgttccgg caagccggga ggcccgagca ctttgcccga ggtccgggcg aaaatggtgg
       61 cgagtcggcc tgtacgccgg attctgtccg gggtgtctct cgacgccccg gggcggccat
      121 ccctctcgac cgacggttgc ccgccggctc tagcagccca cccgggggtg tctacggacg
      181 ggcgtcctcc ccctgtctgg ccttgctccc ggtggggttt gcctagccac ccgggtcacc
      241 ccgggtgctg gtgcgctctt accgcaccgt ttcacccttg cctgtgcggc tttcgtcgcc
      301 atcggcggtc tgttctctgt ggcactgtcc tgcgggtcgc cccgactggg tgttgcccag
      361 caccgtgccc ggtggagtcc ggacgttcct cggaccggtt ggaccggtac gcggccgccc
      421 ggccgactcg ccaccgccac cattgtctca cgatcccacg gcgactgagc ccgcagatgc
      481 tggtcgggcc ggctgagccc cggaccctcg ctggccccta cgctctcggc cccatggctg
      541 ttcccgctgc tcttgcccgt gccgagacgc agctgatcga ccgcgacgag cctctcaccc
      601 gcgacgaggt cgccgcggtg ggggcccttc cgctcgacac ccttcctcac ctcgtggccc
      661 tcgcccaccg ggtacgactc gcctactgcg gtccgtcggt cgagctcgag agcctcatca
      721 acgcgaagtc cggggcgtgc gccgaagact gtgcgttctg ctcccagtcg gctcggttcc
      781 acaccgacgt cgacgtgtac ccgttcctcg acctcgacga ggtcatggac gcggcccgag
      841 ccacccgcgc cgcgggggcg acacagttct gcatcgtggt cgcggtccgc aaccccgagg
      901 aacgcctcct ccggaaggtg atcgacgcag ctgccgcggt gcaacacgac gtcggcttgg
      961 aggtcgcgtg ctcgctcggc ttgctgaccc aagagcaggc ggagcgcctc gccgcggctg
     1021 gcgtgcggcg ctacaaccac aacctcgaga cctgccgggc ggtgttcccg cagatctgca
     1081 cgacccacac ctacgacgac cgtgtggaca ccgcccgact cgcgatcgag gccgggatgg
     1141 agctgtgctg cggtgggatc ctcggactgg gtgagacgtt ggagcaacgg ctcgacttcg
     1201 cgttcgagct cgcggccctc gatccgtgcg aggtgccgat caacttcctc gacccgcgcc
     1261 ccgggacgcc gctcgcggac caggcgctcc tctcaccccg ggaggcgctc caagccatcg
     1321 cgctgttccg actggtgctc ccccgggcgt ggctgcgtct ggccggcgga cgagagctgg
     1381 tcctcggcga gctgcagtcg atgggcctgc tcgcgggtgc gaacgccttg atcgtcggga
     1441 actacctgac cacgacaggc cgaggcgcgg acgaggaccg ggcgttgctc gacgcgttag
     1501 ggatgccggt agccgacggc ccgggcgagg gccgcttcgt ggtcgacgcc gacggagccc
     1561 accctcgacc agcgcgtcgc ccgatcgcgg tgcacgccga gtaggcacgc ccgaacggcg
     1621 cgcccgccgc gaaccgctga cggtcagccg agaaggtggc cggtctcgtt gaagctcagc
     1681 aggaccggcc cgttcaaccc ggcgaccagg cgtgagatcg acgcgacgtc gaggcggagg
     1741 cgccacgcgt agctgtcgtc gaggtccagg gcccagcaga tgatcgcctt gatcggggac
     1801 acgtggctca ccacgatgat gaccccgtcg ctcgccagct ccatgagccc ttccgcacac
     1861 ggcgtgaccc gcgaccggag atcgacgagg ctctcgccac ccggcgcggc gaagctcgga
     1921 tcggatcgcc agcgcgtacc aacgtcggcg gggatgtcgc gcaacagccg ttcgtcccac
     1981 tcgccgtagt cgagctcgac gagccgctcg tcgatccgca ccggggtccc ggtcgcggcg
     2041 ctgacccgct ccgcggtctc gcgggcgcgc agcagcgggc tcgtcagaac caggctcggg
     2101 gcgggaccgt gctcgatcac cgtcgcgagc gcgtcggcct gatgacggcc gcgttcgttg
     2161 aggggagggt cagcacgtcc gaggagccgc ccggcccggt tggcctcggt ctggccgtga
     2221 cggacgagga cgatcacggc tcaccctcgc ggagccgccc gccatgccag aagtcccgcc
     2281 gaggctgggc caggtagaga tcgaactggc cgacgaccca ccaccacgcg gcgacgccgg
     2341 cgagctcctc gaggacgacc gcccaccaga cgggaagcaa cgacaggtgg cccgcgccgc
     2401 cgaggaacgg ccacaggaac acgtggtcct gggtgaacgc acccgagagg accagcgcga
     2461 cgaagacacc gatcgggacg cagagcagcc ggcgacgcag cagccgggac cgccccgccg
     2521 tgaccaccat gaccgcggcg aagagcccga cggggaacag gagcgcgtgg ccgaacgccc
     2581 ggtgcccggc cggcaggtcg acgatcacgg ggagcagcgc accgacggcg acgagccggt
     2641 agtccagacc ggtgcttcgg aacacgttgg cgacgatcag gatcgacagc cccgtgtacc
     2701 agaacagcac cccgagtcaa ctttggtcga gggcggcgtt cacgagcaga tccgcgtcga
     2761 cgttctgctc gcgccgcacg tgggccagat caaccgccgc gtagctgtcg aggagcgctt
     2821 tcgcttccgc gtgcaagggg cggaggtggg gctgcttgat ccgccacaca ccctcaagct
     2881 ggcggatcac gagcatcgag tcggcccgga gatgcacgac ccgggcgtgg tacgggcggg
     2941 cggcttggag gccggcgatg acggcccggt actccgcgac gttgttggtg gcgaccccga
     3001 tccgctcgct caccgtcgcc agctgggtcg gcggctcggt cgtgggatcg gtgaccacgg
     3061 ccccgatcgc ggccggcccc gggttcccgc gggcaccacc gtcacagaag atggtcacct
     3121 cgtcgtgacg gttcacggga cgaggatgca gccgcagttg tcgcagaacg cgagcgctcc
     3181 gtccccctgc ttgcggatcc gatcgacctc cgtcgccggg atcgacagat gacagccctg
     3241 acacgtcatg cccgagagtc gcgccgcgcc gaccccgttg ccccgctcgc ggcgccgctc
     3301 gaactccgag accagcccgg cgtcgatcgc ccccgcgagc tcgtcgcgcg ccgcccgctc
     3361 caccgcggcc gcctcgtcca gctcacgctc acgggcttcg aggtcacgtt gcagcaccgc
     3421 ggcctcggac tccagggaag cgccgtcggc gtcaagggcc ccgacggtcg cgtcgagggg
     3481 ctcgcgctgc tccatcgccg atagctgacg gtcctcgacc tcacgctggt ggcgacggag
     3541 ctgctcgacg tcggcttgga gcgcctgaag ctcccgaggc gagctcacct cccccgagta
     3601 caggcgctgc tcggccgccg cggcctgctc ccccaaacgt tgtgcctcgt cggcgaggcg
     3661 ctgctcgcca cccgcgacgt cgtcgcgttc ggcctgctg
//
LOCUS       DATCCH010000229         2064 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_191144, whole genome shotgun sequence.
ACCESSION   DATCCH010000229 DATCCH010000000
VERSION     DATCCH010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191144"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..984
                     /locus_tag="VIJ44_04510"
     CDS             <1..984
                     /locus_tag="VIJ44_04510"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5165196.1"
                     /translation="DRDRRFPTVRWRSRAGGLATLNREWTETYARYSSRGWDPEAYVL
                     AMDDQGIDRMALYPSRGLMQVAAWGLDPALAGAIVRAYNDWAAAFVANIPDRLFAVGQ
                     LDLRDADGAIAEARRCVDQHHFRGFFVLPEPPLPGVTLERAYYDPLWSTLEELDVALA
                     LHNVAGTGLGQLGADRFGEWALPRIAFAFPLEAQMTLFSFLVGGICDRHPGLRVVVLE
                     SGAGWLPFWLWWLDELYERYVGLDCDELSATPSELFRRHCFVSAEIEEPGLRAVIDAL
                     GVDCVVTASDFPHPEGSFPDGIRDFVARGDLGESEKRRILWDNPRRLYGVG"
     gene            complement(985..>2064)
                     /locus_tag="VIJ44_04515"
     CDS             complement(985..>2064)
                     /locus_tag="VIJ44_04515"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY5165197.1"
                     /translation="SIDPALSDPDRTVTPLSFEAVQAVLKDGDTFSSAAYADSMGLVM
                     GHSILEMDEPEHHRYRSLIQQAFTRKAMDRWEVELVGPIVNGLVDEFVDRGRADLVRQ
                     LFWPFPVHVIAGMLGLPAEELPDFHRRAVELISIAVDIDRGLVASQWLYDFFAGVIEQ
                     RRADPREDLISVLVEAELDGQRLTDEEIIAFLRLLLPAGAETTYRSSSNLMYGLLSNP
                     DQLERLRADRALMPAAIEEGLRWEAPLTGIARTATRDVTIDGVEIPAGSPVSVCLGGA
                     NRDPSRWDHPDQFDLARDPKQHLAFAFGPHMCLGMHLARMETTVAINAVLDRLPALRL
                     DPSADDLHITGLMFRAPKALPVLFD"
BASE COUNT          299 a          669 c          762 g          334 t
ORIGIN      
        1 gaccgggatc gccggttccc gacggtgcgg tggcgaagcc gcgccggcgg gctcgccacc
       61 ctgaatcggg agtggaccga gacctacgcg cggtactcgt cgcgcggatg ggacccggag
      121 gcgtacgtcc tcgcgatgga cgaccagggc atcgaccgca tggccctgta cccaagtcgc
      181 gggctcatgc aggtcgcggc ctgggggctc gatccggccc tcgccggcgc gatcgtccgg
      241 gcttacaacg actgggcggc ggcgttcgtc gcgaacatcc ccgaccggct cttcgccgtg
      301 ggccagctcg acctgcgcga cgccgatggt gccatcgctg aggcccgacg gtgcgtcgac
      361 cagcaccact tccggggctt cttcgtgctt cctgagccgc cgctgccggg tgtgacgctg
      421 gagcgggcgt actacgaccc gctgtggtcg acgctagagg agctcgacgt ggcgctggcg
      481 ttgcacaacg tcgccgggac cgggctgggt cagctcggcg cggaccggtt cggtgagtgg
      541 gcccttccca gaatcgcgtt cgcgtttccg ctcgaagcgc agatgacgtt gttctcgttc
      601 ctcgtcggcg ggatctgtga ccggcacccc gggttgcgag tcgtggtgct cgagtccggg
      661 gcggggtggc tgccgttctg gctgtggtgg ctcgacgagc tgtacgagcg ctacgtcggt
      721 ctggactgcg atgagctgtc cgcgactccg agcgagctgt tccgtcgcca ctgtttcgtg
      781 tcggccgaga tcgaggagcc cgggctccgg gcagtgatcg acgcgctcgg cgtcgactgc
      841 gtcgtgaccg cgtccgactt cccgcacccc gagggatcgt tccccgacgg gattcgtgac
      901 ttcgtggcgc gaggcgacct cggcgagtcc gagaagcgac gcattctctg ggacaacccg
      961 cgccggttgt acggcgtcgg ctgatcagtc gaacaggact ggcagcgcct tcggggcccg
     1021 gaacatgagg ccggtgatgt gcagatcgtc agcgctcgga tcgagtcgca gcgcggggag
     1081 ccgatcgagg acggcgttga tcgcgacggt ggtctccatg cgggcgagat gcatcccgag
     1141 gcacatgtgc ggcccgaacg cgaacgccag gtgctgcttc gggtcccggg ccagatcgaa
     1201 ctggtcgggg tggtcccagc gggacggatc gcggttcgcc ccgccgaggc agacactgac
     1261 cggtgacccg gccgggattt cgactccgtc gatggtcacg tcgcgcgtcg cggtgcgcgc
     1321 gatgccagtg agcggcgcct cccaccgcag accctcttcg atcgcggccg gcatgagggc
     1381 ccggtcggcc cgcagccgtt ccagctggtc ggggttggag agcagcccgt acatgaggtt
     1441 gctcgacgac cggtaggtgg tctccgcgcc ggccggcagc aggagccgca ggaaggcgat
     1501 gatttcctcg tcggtgaggc gctgaccgtc gagctcggcc tcgacgagga cgctgatcag
     1561 gtcctcgcgc ggatcggccc ggcgctgctc gatcacgccg gcaaagaagt cgtacagcca
     1621 ctgcgaggcc acgagcccgc ggtcgatgtc gacggcgatg ctgatgagct cgacggcgcg
     1681 acggtggaag tcgggaagct cctccgcggg gaggccgagc atccccgcga tcacatgcac
     1741 cgggaagggc cagaagagct ggcgcaccag gtcggcacgg ccccggtcga cgaactcgtc
     1801 gacgagtccg ttgacgatcg gtccgaccag ctcgacctcc cagcggtcca tcgccttgcg
     1861 ggtgaaggcc tgctggatca gggaccggta gcggtgatgc tccggctcgt ccatctcgag
     1921 gatcgagtgg cccatgacga ggcccatgct gtcggcgtac gccgccgacg agaacgtgtc
     1981 accgtccttg aggacggcct ggacggcctc gaaggacagc ggagtgaccg tgcggtcggg
     2041 atccgacagg gcggggtcga tcga
//
LOCUS       DATCCH010000230         5815 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_191185, whole genome shotgun sequence.
ACCESSION   DATCCH010000230 DATCCH010000000
VERSION     DATCCH010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5815
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191185"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..1090
                     /locus_tag="VIJ44_04520"
     CDS             122..1090
                     /locus_tag="VIJ44_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020551617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5165198.1"
                     /translation="MLSYVGRRALYSIPVILVASFLMFAFVRATFDPTARLRSSREAA
                     PLIRAERERLGLNRPLVDQYGSWLSKFVRGDWGTSERTHEAVTAQIGRALGNTTQLIF
                     WGVLLSALVAVTIGVYSALRQYSVLDYTFTGLSFIGLALPPFWFGLIAIEFLAVGPKQ
                     WFHLSEAPLFFVGLHSGSSGFGLDYARHLVLPVLTLTVQIVAGWSRFQRASMLDVMSS
                     DYIRTARAKGVPRRRVVLRHGLRNALIPLVTVMALDIGALSGGLIITEKIFSIDGMGK
                     LFFDSLLNGDATTLTAWLVVTALFIVAFNLVADLAYGWLDPRIRVA"
     gene            1087..2043
                     /locus_tag="VIJ44_04525"
     CDS             1087..2043
                     /locus_tag="VIJ44_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019011518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5165199.1"
                     /translation="MTPPTHAGPVVPEPGPVGPSELLPSAAAPTIGGAPEHRSPGQLF
                     WRRFRRHKVALASIGVLVVLCVVCFGASFFAPYSRDHQNLTLGTVGPSWSHWFGTDEL
                     GRDQLSRILYAGQISLRIGLAVALLSTLVGTIVGAVAGYLGKATDQVLMRTTDLFLIV
                     PGIAVLALALKKFGRDDVTIIWVLAALFWMYVARVVRAQVLSLKEQEFVEAARASGAS
                     GPWIVIRHILPNCIGPIVVNATLAVAAAIVAESTLSFLGFGVQLPATSWGRMLSDAEG
                     YVGTSQSYLLYFPGLAILLTVLAVNFLGDGLRDAFDPQSEPR"
     gene            2040..3080
                     /locus_tag="VIJ44_04530"
     CDS             2040..3080
                     /locus_tag="VIJ44_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019074515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5165200.1"
                     /translation="MSGAATSPGPLLEIDGLSVTFATDDGPLHAVDNLTLTVEDHEVL
                     GVVGESGSGKTVTALAILGLLPKRATIRGSIRFRGDELVGRSERDLQAIRGREIAMIF
                     QDALAALNPVMTVGKQIAEAITVHETGLSADDVRDRVVALLDLVGITDPEGRARRYPH
                     EFSGGMRQRAMIAMALANDPALLIADEPTTALDVTLQAQVLDVLERIRDRTGSSILLI
                     THDLGVVAGVAERVAVMYAGRRVEEAPVDELFAEPRHPYTLGLLASLPRLDRPARDGT
                     LFRIPGQLPSLIHRPEGCAFHPRCAFRALPVPCATEVPADVTIGAAHRSACHRVADLD
                     GVTPDDLRLRGA"
     gene            3077..4096
                     /locus_tag="VIJ44_04535"
     CDS             3077..4096
                     /locus_tag="VIJ44_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017587005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEY5165201.1"
                     /translation="MTTLSSVVPPVDDRPGGALLEVRDLVKDFPVRAGWRRRPVGQIS
                     AVAGVSFAVPRGQTFALVGESGCGKSTTARLVLRLVPATSGTVRFDGVDVLGASPAEL
                     RRLRARMQIVYQDPYASLNPRLTVGGIIADPLRVHDRWSNGGPERVQTLLEQVGLNPT
                     DVNRYPHEFSGGQRQRIGIARALALEPDLLVLDEPVSSLDVSIQAGVINLLDELQRHL
                     GLTYVFIAHDLAVVRHVADHVGVMYLGKLVEHGPADQVYDHPQHPYTQALLSAVPVPD
                     PPVERRRRRILLTGDLPNPGDPPSGCRFRTRCWRAQERCGQHEPPLEPDGDGHAVACY
                     FPGPE"
     gene            4131..4215
                     /locus_tag="VIJ44_04540"
     tRNA            4131..4215
                     /locus_tag="VIJ44_04540"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4165..4167,aa:Leu,seq:gag)
     gene            complement(4329..4607)
                     /locus_tag="VIJ44_04545"
     CDS             complement(4329..4607)
                     /locus_tag="VIJ44_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011785982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HEY5165202.1"
                     /translation="MKKLTKIPRVTSVEVRPPYGLHLRFDDGLTRDVDLADELWGPMF
                     EPLKEPAFFAQVSVEHGTVVWPNGVDLDPLVLHGDAEPAVSRSERQTG"
     gene            complement(4604..4900)
                     /locus_tag="VIJ44_04550"
     CDS             complement(4604..4900)
                     /locus_tag="VIJ44_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008050826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HEY5165203.1"
                     /translation="MSLSQGIGTLRDVPRISAFYGVVIYIYWRDHAPPHFHAEYADQE
                     ALIVIGDGHLYAGSLPPRALRLIAEWRRLHLDELQQAWELASRHEDPGTIEPLP"
BASE COUNT          859 a         2119 c         1848 g          989 t
ORIGIN      
        1 cccctcgacc cgctccccaa catcggggtg tggagcaacc acatcggcgg cgtccacggc
       61 gacaacccgg tgttcgcgat cttctggaac ctcagccagt ggcaggccca gtagccacgc
      121 cgtgctgagc tatgtcggcc ggcgcgccct ctactcgatc ccggtcatcc tcgtcgcgtc
      181 gttcctcatg ttcgcgttcg tgcgcgccac gttcgacccc acggcgcggc tgcgttcctc
      241 ccgcgaggcc gcgccgctga ttcgagccga acgggaacgg ctcggcctga accggcccct
      301 cgtcgaccag tacgggtcgt ggctcagcaa gttcgtccgc ggcgactggg ggacgagcga
      361 gcgcacccac gaggcggtca ccgcgcagat cggacgggcg ttgggcaaca ccacccagct
      421 catcttctgg ggggtgctcc tctccgcgct cgtggcggtg acgatcggcg tctactccgc
      481 gctgcgccag tactcggtgc tcgactacac cttcaccggg ctgtcgttca tcgggctcgc
      541 cctcccgccg ttctggttcg gtctcatcgc catcgagttc ctcgccgtcg gacccaagca
      601 gtggtttcat ctctccgagg cgccgctgtt cttcgtcggg ctgcatagcg ggtcgagcgg
      661 gttcgggctc gactacgccc gccacctggt gctcccagtc ctcacgctaa cggtgcagat
      721 tgtcgccgga tggagccggt tccaacgggc gtcgatgctc gacgtcatgt cgtccgacta
      781 catccggacc gcacgcgcca aaggggttcc ccgccgtcgg gtggtgctgc gtcacgggct
      841 tcgcaacgcg ctgatcccgc tcgtcaccgt catggccctc gacatcggcg ccctgtccgg
      901 agggctgatc atcaccgaga agatcttctc gatcgacggg atgggcaagt tgttcttcga
      961 ctcgctcctc aacggtgacg ccacgaccct caccgcgtgg ctggtcgtca ccgcactgtt
     1021 catcgtggcg ttcaacctcg tcgccgacct cgcctacggt tggctcgacc cccggatccg
     1081 ggtcgcgtga caccgcccac ccacgccggg cccgtcgtcc ccgaaccggg gccggtcggc
     1141 ccgtccgagc tgctcccctc cgccgcggcg ccgacgatcg gaggcgcgcc ggagcaccgc
     1201 tccccggggc agctgttctg gcgccgcttc aggcgccaca aggtcgcgct cgcctcgatc
     1261 ggggtcctcg tcgtcctctg cgtcgtctgc ttcggcgcgt cgttcttcgc gccgtactcg
     1321 cgtgatcacc agaacctgac cctcgggacc gtcggaccgt cctggtcgca ctggttcggg
     1381 accgacgagc tgggccggga ccagctctcc cgcatcctct acgccgggca gatctccttg
     1441 aggatcgggc tcgcggtcgc gttgctctcc accctcgttg ggacgatcgt cggtgccgtt
     1501 gcgggctacc tgggcaaggc caccgaccag gtgctcatgc gcaccaccga cctgttcctc
     1561 atcgttcccg gcatcgcggt gctcgcgttg gcgctgaaga agttcgggcg cgacgacgtc
     1621 accatcatct gggtgctcgc ggcgctgttc tggatgtacg tcgcgcgggt cgtgcgcgcc
     1681 caggtgctgt ccctcaagga gcaggaattc gtcgaagcgg cccgggcgtc gggcgcgtcg
     1741 gggccctgga tcgtgatccg ccacatcctg cccaactgca tcggacccat cgtcgtgaac
     1801 gccaccctcg cggtcgccgc ggcgatcgtc gccgaatcga cgctgtcgtt cctcggcttc
     1861 ggggtacagc tccccgccac gtcgtggggc cggatgctct ccgacgccga aggctacgtc
     1921 ggcacctccc agtcctacct gctctacttc ccgggtctgg cgatcctgct caccgtcctc
     1981 gcggtcaact tcctcgggga cggcctccgc gacgccttcg acccgcagag cgaaccgcgg
     2041 tgagcggggc tgcgacctcc ccgggaccgt tgctcgagat cgacgggctc agcgtcacgt
     2101 tcgccaccga cgacgggccg ctccacgccg tcgacaacct gaccctcacc gtcgaagacc
     2161 acgaggtgct cggcgtcgtc ggcgaatccg gttcgggcaa aaccgtcacc gcgctggcca
     2221 tccttggcct gcttcccaag cgcgccacca tccggggatc gatccgcttc cgcggcgacg
     2281 agctcgtcgg gcgctccgag cgcgacctcc aagccatccg ggggcgggag atcgccatga
     2341 tcttccaaga cgccctggcc gccctcaacc ccgtgatgac cgtcggcaag cagatcgccg
     2401 aggccatcac cgtccatgag accgggctca gcgccgacga tgtccgggac cgggtcgtcg
     2461 cgctcctcga cctggtcggg atcaccgacc ccgaaggacg ggcccgacgg tacccgcacg
     2521 agttctcggg tgggatgcgc caacgggcca tgatcgccat ggccctcgcc aacgaccccg
     2581 cgctgctcat cgccgacgag cccaccaccg ccctcgacgt caccctccaa gctcaggtgc
     2641 tcgacgtcct cgagcggatc cgcgaccgca ccggctcgtc catcctcctc atcacccacg
     2701 acctcggagt ggtcgccggg gtcgccgagc gcgtcgcggt gatgtacgcc ggacggcgcg
     2761 tcgaggaggc cccggtcgac gagctcttcg ccgagccccg ccacccctac accctcgggt
     2821 tgctcgcctc gctgccccgc ctcgaccggc ccgcgcgcga cggcaccctg ttccgaatcc
     2881 cgggccagct cccctcgctg atccaccggc ccgaaggctg cgccttccat ccccgctgcg
     2941 cgttccgtgc ccttcccgtc ccgtgcgcca ccgaggtgcc agccgacgtc accatcggag
     3001 ctgcgcaccg ctccgcgtgt caccgcgtcg ccgatctcga cggcgtcact cctgacgatc
     3061 tccggctgcg cggcgcgtga ccaccctgtc gtcggtcgtg ccaccggtcg atgaccggcc
     3121 cggtggtgcg ctgctcgagg tgcgcgacct ggtcaaggac ttccctgtaa gggccggctg
     3181 gcgccgccga cccgtcgggc agatcagcgc agtggcgggg gtcagcttcg cggtgccccg
     3241 cggccagacc ttcgcgctgg tgggggagtc cggctgcggc aagtcgacca ccgcgcgcct
     3301 cgtgctgcgc ctcgtccccg ccacctcagg gacggtgcga ttcgatggcg tcgacgtgct
     3361 tggcgcatcg cccgcagagc tgcggcgact ccgggcccgg atgcagatcg tgtaccagga
     3421 cccgtacgcg tcgttgaacc cccgcctcac cgtcggcgga atcatcgctg accccctccg
     3481 cgtgcacgac cggtggtcca acggcggtcc tgaacgggtg cagaccctgc tcgagcaggt
     3541 tgggcttaac cccaccgacg tgaaccggta cccgcacgag ttctccggcg ggcagcgtca
     3601 acgcatcggc atcgcccgcg cactggccct cgaacctgac ctgctcgtcc tcgacgagcc
     3661 cgtcagctcc cttgacgtct ccatccaagc cggggtgatc aacctcctcg acgagctcca
     3721 acgccacctc ggcctcacct acgtgttcat cgcccacgac ctcgccgtcg tccgccacgt
     3781 cgccgaccac gtcggcgtca tgtacctcgg caagctcgtc gagcacggcc ccgccgacca
     3841 ggtctacgac cacccccagc acccgtacac ccaagcgctc ctctcggcag tccccgtccc
     3901 cgacccgccc gtcgagcgcc gacgacggcg catcctcctc accggcgacc tccccaaccc
     3961 tggcgacccg ccaagcggct gtcggttccg cacccgctgc tggcgcgccc aagagcgctg
     4021 cgggcaacac gagccaccac tcgaaccgga cggcgacgga cacgcggtcg cgtgctactt
     4081 ccccggaccc gaatgacagc gacacccctc cggtaaactg cccgactccc gtcggagtgg
     4141 cggaatcggc agacgcgcta gcttgagggg ctagtgcccc aaaagggcgt gggggttcaa
     4201 gtcccccctc cgacacgctg aggcttgggg tggactgcct cgaccctcaa gacggtgaga
     4261 ggcgcggggc gctgctcggt gctcagctcg gacgcacacc cgaaacgacc tgtcacttac
     4321 gggggcaact agccagtctg gcgttcggac cgggatacgg ccggttccgc gtcgccgtgc
     4381 agaacgagcg gatcgagatc aacgccgttt ggccagacga cggtgccatg ttcgaccgag
     4441 acctgggcaa agaaggccgg ttctttcagc ggttcgaaca ttgggcccca gagttcgtcg
     4501 gccaagtcga catcgcgcgt gaggccatcg tcgaacctca ggtgcagtcc atacggtggc
     4561 cgcacttcga cggaggtaac tcgggggatc ttggtgagct tcttcatggc agcggctcaa
     4621 tagtaccggg atcttcgtgg cgggacgcga gttcccacgc ttgctggagt tcgtcgagat
     4681 gcaggcgacg ccactccgcg atcaatcgaa gcgctcgtgg tggcagtgac ccagcgtaga
     4741 ggtggccgtc accgatcacg atcagagcct cctgatcggc gtactcggcg tggaaatgcg
     4801 gcggagcatg gtctcgccag tagatgtaga tgacgacgcc atagaacgcc gagattcgcg
     4861 gcacatctcg gagagtaccg atgccctgtg acaggctcat cgtgccgaca gtgggccgcg
     4921 gcgacgcggt gaaggtcctt ccaaccggat cacagtgccg ggcgacccgc cgcgtcggag
     4981 ctcgatagtc cgctggcgca ttcgggtccc tggggcgccc gacactccct atgggagtag
     5041 cagctccgcc cggagcagcg gtggactttc gggttgactc gcccctccga cacgatgtga
     5101 tgtcgcaaga catcggagag actgctgtcg cgagtcatcg tagacagcgg gctcattctt
     5161 ggggctggta atcgtgggtg gggtcgaggg tgagcctgcg gaggagttcg ccggttgtgg
     5221 tcgtcgagga tgcggatgtc gcgtccggag acgagcatgg cgacggtctt ttggacgtgg
     5281 cggcgcctca tgccgatgtg gtggagcgac accacaaccg acgcgtgcac cattggctgt
     5341 agcgagcgag cagttcttgg cgatgaggat cttgtcgacc tcgtcgggcc gtcggcgatc
     5401 cgcagccgcg cgctctctgg cacatgcccg cgaggggacg gtcatccgag acgccggcgg
     5461 cgcccgacac ctggatcgcg cggtcgacca cgtgctatac gcgggcgggg gcgaatatct
     5521 cgatcgcgag gatgtcggtt cacgagtcaa cccgcttcgc atctcctcga cgcagtggat
     5581 tgtgatcaga cgcgccgcct tgatgtcgac gtaggctccc cggctcaccg gcggcaacgt
     5641 cgcaagaagc gcgaacgggc ccggtgctgt taccccgtta acactggtgt cttgcgggga
     5701 cccgacgcca accagcgctg aaatagctca acccgtgccg cggcgtgctc atgcgagaac
     5761 ttgcaggtgt gcacttcacc gcactgggag ttgctggcgc ccgtggcgaa gttga
//
LOCUS       DATCCH010000231         2578 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_108281, whole genome shotgun sequence.
ACCESSION   DATCCH010000231 DATCCH010000000
VERSION     DATCCH010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108281"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..521)
                     /locus_tag="VIJ44_04555"
     CDS             complement(144..521)
                     /locus_tag="VIJ44_04555"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY5165204.1"
                     /translation="MSQRAGWPIDDLARVAGVTARNLRAYQSLGLLAAPRLAGRVGLY
                     DAEHLDRVRAIRRLQARGFSLAGVKELFDAHDRGQTLEALLGLDPSLLTASADAADAR
                     LPRLALVPGPWAQELGTPSPAAN"
     gene            565..1140
                     /locus_tag="VIJ44_04560"
     CDS             565..1140
                     /locus_tag="VIJ44_04560"
                     /inference="COORDINATES: protein motif:HMM:TIGR03086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03086 family metal-binding protein"
                     /protein_id="HEY5165205.1"
                     /translation="MGEQGRVLTERHRRSCQGFSAVVQAVRADGWADPTPCSEWDARA
                     LVEHVIGFHEFLLLRPLGVGAERPREGPAARWAATELAIELALGHPGLSRQVAYFDGD
                     TRRPVDVLGAITGDVVIHTWDLARAVGAPERLDADLCATALDAARATTGASDRSGLFA
                     PPILTRPDASTQDCLLALRGRDPQWCPAALT"
     gene            1159..1557
                     /locus_tag="VIJ44_04565"
     CDS             1159..1557
                     /locus_tag="VIJ44_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5165206.1"
                     /translation="MLGHLGINVPDLEGARSYYDRLLPFLGFEPLLAQEHQLAYRPAA
                     GKPGTYLFFYPAATNADYEQDRPGLQHLAFMVPTRSEVRAAHQRVLDLGGDVLHPPRT
                     FPEYPPPYYATFWLDPMGLTLEAVCHHDRD"
     gene            1645..2214
                     /locus_tag="VIJ44_04570"
     CDS             1645..2214
                     /locus_tag="VIJ44_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165207.1"
                     /translation="MRSGTSPMTRELYRGGERIREFDVRSAATVMSAFIGGLFLVFFL
                     AAFLPWLLSKGMTAAVEGLMNRIGFGTFHANTIWSWLLVLAMFALFTAFIVVIATGIL
                     ALYNLLSERTGIGMPVEGGTRELVAGGRAPLAPPDDDETDGSFDELYAEAQRRNLKGR
                     STMSKGELQKALRRRRVGARPTKSRTRRV"
     gene            complement(2252..2322)
                     /locus_tag="VIJ44_04575"
     tRNA            complement(2252..2322)
                     /locus_tag="VIJ44_04575"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2288..2290),aa:Gly,seq:tcc)
     gene            complement(2346..2422)
                     /locus_tag="VIJ44_04580"
     tRNA            complement(2346..2422)
                     /locus_tag="VIJ44_04580"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2386..2388),aa:Pro,seq:tgg)
BASE COUNT          384 a          952 c          862 g          380 t
ORIGIN      
        1 tcaaatgaca gccgtgcaac ttcaccaatg aggctacggg cttcccctca gccctcgaaa
       61 ccgtgccgtc ccgaatgtcc gaattgccgg cccggtgtga tggaagtcac gtccgccacc
      121 cccggccagg gccagcaggg gcgctagttc gcggccgggc tcggtgtccc gagctcctgg
      181 gcccagggtc cgggaaccaa cgccaggcgg gggaggcgag cgtccgcggc gtcggccgac
      241 gcggtcagca gggacggatc caggcccagg agcgcctcga gggtctggcc gcggtcgtgg
      301 gcgtcgaaga gctccttgac gccggccagg gagaaccctc gcgcctgcag acgccggatg
      361 gcccggaccc ggtcgagatg ctcggcgtcg tagaggccga cccgccccgc caggcgagga
      421 gccgccagga ggcccaagga ctggtaggcc cgaaggttcc tcgccgtcac gcccgcgacg
      481 cgggccaggt cgtcgattgg ccagcccgcc ctctgagaca tatgctcatg ttacatagat
      541 tgatgacagc gagagggggg gcggatgggg gagcagggtc gcgtcctcac tgaacggcat
      601 cggcggtcct gccaggggtt cagcgcggtg gtgcaggcag tccgggcgga cggctgggct
      661 gacccgaccc cctgctcgga gtgggatgcc cgggccctcg tggagcacgt catcggcttc
      721 catgagttcc tgctgctccg cccgcttggc gtgggcgccg aacggccccg ggagggcccg
      781 gccgcgcggt gggccgccac cgagctcgcg atcgaactgg cgctgggtca ccccgggctg
      841 agccggcaag tcgcctactt cgacggcgac acccgccgac ccgtcgacgt gctgggcgcc
      901 atcaccggcg acgtcgtgat ccacacctgg gatctcgctc gagccgtcgg cgccccggaa
      961 cggctcgacg cggacctgtg cgcgaccgca ctcgacgcgg cacgagcaac gacgggtgcc
     1021 tccgaccgct ccggtctgtt cgccccgccg atcctgaccc gacccgacgc cagcacccaa
     1081 gattgcctcc tcgccctgcg gggccgcgac ccgcaatggt gccccgcggc cctgacctga
     1141 cccgcccgag gaccccccat gctcggacat ctcggcatca acgtccccga cctcgagggc
     1201 gctcgcagct attacgaccg gctcttgccg tttctcggct tcgaaccgct cctggctcag
     1261 gagcaccagc tcgcctaccg gcccgcggcc ggcaagcccg ggacctacct cttcttctat
     1321 cccgcggcca ccaacgccga ctacgaacag gaccggcccg gcctgcagca tctggcgttc
     1381 atggtcccga cccgatcgga ggtgcgcgcc gcgcaccagc gggtgctcga cctcggcggc
     1441 gacgtcttgc acccgccgcg aaccttcccg gagtacccgc ctccgtacta cgccaccttc
     1501 tggctcgacc cgatggggct cacgctcgaa gccgtctgtc accacgaccg ggactgaccc
     1561 accacccgcg gcgcgtccgc tccgacgccg accggtcaac gccgggctcg aagcgagcgt
     1621 ccgggaactg cgtagcctgg gccgatgcgt tccggcacga gccccatgac cagagagctg
     1681 taccgaggcg gcgagcggat ccgcgagttc gacgtgcgat ccgccgcgac tgtcatgagc
     1741 gcgttcatcg gcggcctgtt cctcgtcttc ttcctcgccg cgttccttcc gtggctgctg
     1801 tcgaagggaa tgaccgccgc cgtcgaaggc ctcatgaacc ggatcggctt cgggaccttc
     1861 cacgccaaca cgatctggag ctggctgctg gtcctcgcga tgttcgcgct gttcaccgcg
     1921 ttcatcgtcg tcatcgcgac cgggatcctc gccctctaca acctgctgtc agagcgcacg
     1981 gggataggca tgcccgtcga aggcgggaca cgcgagctgg tcgccggagg ccgcgcgccg
     2041 ctcgccccac ccgacgacga cgagaccgac gggtccttcg atgagctcta cgccgaggcg
     2101 caacgccgga acctcaaggg ccggtcgacg atgtccaagg gcgaactgca gaaagcgctg
     2161 cggcgtcggc gtgttggtgc tcgtccgacc aagtcccgga cccgtcgcgt ctgacttcgc
     2221 cgatcgcgac gacgtgagcg ccggcgatgg gagcgggtga cgggaatcga acccgcatca
     2281 ccagcttgga aggctggggc tctaccattg agctacaccc gcgtggacgc acaccgtcgc
     2341 ttggttggtc ggggaggggg gatttgaacc cccgaccgcc tgcccccaaa gcaggagctc
     2401 tgccaagctg agccactccc cgtgcgcgct gcgcctcacc gcgatcctag gtgagcgatg
     2461 cctcacggcc ggcgcccacg tccgcgcggg tcctccatcg agggtcggcc ggagcatcgg
     2521 gttgctgccg ccggcacgcc ttcgccgccg gccccgggtc cagtcccggg tcgtgggg
//
LOCUS       DATCCH010000232         4037 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_273954, whole genome shotgun sequence.
ACCESSION   DATCCH010000232 DATCCH010000000
VERSION     DATCCH010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4037
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273954"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /locus_tag="VIJ44_04585"
     CDS             <1..751
                     /locus_tag="VIJ44_04585"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY5165208.1"
                     /translation="DRHQMFEWSNRLIGSQDPEYAVSAEDATGAALEMFSYANDLAAA
                     KRAKPGDDIVSTLLAAEVEGERLTDAEFDLFFELLAVAGNETTRNLISHGMLALAENP
                     DQRDLLLADPSLLPGAVDEMLRYASPVMYMRRTARADIELHGQQIKEGDKVALWYIAA
                     NHDPAVFTHPHRFDIRRSPNDYLAFGGGGPHFCLGSHLAKLEIRVMFEELLARLPDVE
                     LDGPVQRLRSNFINGIKHMPVRFTPRAKAAA"
     gene            844..1791
                     /locus_tag="VIJ44_04590"
     CDS             844..1791
                     /locus_tag="VIJ44_04590"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEY5165209.1"
                     /translation="MPAGGSNLTYRVVDTAGHQWALRRPPVTAVLATAHDVDREWRII
                     EALGRHTDVPVPPAVARCTDPDVSDGPFYVMGFVDGPVLRAHADAAALSADAAQTATD
                     SLIDVQVAFHSVDPDEIGLGDLSRHRTGYVERQLHRWRTQVERAQVRPLPLLDELHDR
                     LARTIPVERAAPGLAHGDYRFDNTILGPDHRVHAVLDWELCTIGDPVADCCWSLLYWS
                     DPGDPSPFLPDAPTLAATFPRRDEVADRYATRSGHDLDTFPWFTVFGYWKMACIVEGV
                     YTRRLQGSRSGTGTQDLGSIARRVESLLDHAAEVATTVC"
     gene            1825..3261
                     /locus_tag="VIJ44_04595"
     CDS             1825..3261
                     /locus_tag="VIJ44_04595"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5165210.1"
                     /translation="MDRRSVVIVGTVSLALLVAIGLGIALTSGGSGNQSVRTPPGSVT
                     TLPIPATLPTTSTIKPVITAPANTVPPPPKPTVVIGPSPTTAAPAPAPAPAPTPTTSP
                     SGGNDTGITDHEIRLTAIADTAPVLRGVEAWASALNRAGGLAKRTVHVDARLVATPAD
                     YSAAVSSACANSFAIVGSSSTFDSQSAGLQCGIPEIATRLFDPVHQSLRNAYAVIPTR
                     ANLERVGAFKRLLSTVSGCCRQSVLVPTTEPGRTATTRSLRGAETIGFTTAATPDVSP
                     TATPADYTAIVADLVAKQATFARSGLGAASTVQLRKAAAANSGASTVKAWYCDASCDD
                     PTFLTAGGAAVEGQLIDVGVNPLVDQHAIPAMATFVRAMRHVGATPSVPALESYSASL
                     LFEQAARQVIQASGTNGLTRVRLLGAVGTMHAFNAGGILGATDVAARQPNGCFMLVTV
                     TNGHFVRSYPAAPGQLDCGAQNLQTGSG"
     gene            3258..>4037
                     /locus_tag="VIJ44_04600"
     CDS             3258..>4037
                     /locus_tag="VIJ44_04600"
                     /EC_number="1.1.1.194"
                     /inference="COORDINATES: protein motif:HMM:NF009092.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coniferyl-alcohol dehydrogenase"
                     /protein_id="HEY5165211.1"
                     /translation="MTPFRYDDQRVVITGASSGMGAATAAMLVEAGAEVHALDIRDVS
                     TPVSKSYETDVGDPDAVNTALAAIGAPIHKLFNVAGVPQTHPPERVFTVNFLGLRHMT
                     ERALDLMPDGGAIANVASLAGMGWSEHVTPIGELLDTPDVAAGQAWAAAHLAEYGDPY
                     FFSKECVIVYTFRLAPRLVREREIRVNCISPGPVDSPMMVDFRAALSSATIDWTAAQA
                     NGRLGRPEEMAPPLVFLNSDDASYVNGHNLVVDAGFTSAMTT"
BASE COUNT          643 a         1549 c         1265 g          580 t
ORIGIN      
        1 cgaccggcac cagatgttcg agtggagcaa ccgcctcatc ggctcccagg accccgagta
       61 cgccgtcagc gccgaggacg ccaccggcgc ggcgctcgag atgttcagct acgccaacga
      121 tctcgccgcc gccaagcgag ccaaacccgg ggacgacatt gtcagcacgc tgctggcggc
      181 cgaggtcgaa ggagagcgtc tcaccgacgc cgagttcgac ctgttcttcg agctcctcgc
      241 ggtggccggc aacgagacga cccgcaacct gatatcgcac ggcatgctcg ccctggccga
      301 gaatcccgac cagcgcgatc tgctcctcgc cgacccgtcg ctgcttccgg gcgccgtcga
      361 cgagatgctc cggtacgcct ccccggtgat gtacatgcgg cgaaccgccc gagccgacat
      421 cgagctgcac ggacaacaga tcaaggaggg cgacaaggtc gcactctggt acatcgccgc
      481 gaatcacgac cccgcggtct tcacccaccc gcaccgcttc gatatccgcc gcagcccgaa
      541 cgactacctc gcgttcggtg ggggcggtcc ccacttctgc ctcggctcgc atctcgccaa
      601 gctcgagatc cgcgtcatgt tcgaagagct gctggcccga ctgcccgacg tggagctcga
      661 cggaccggtg cagcggctcc gctcaaactt catcaacggg atcaagcaca tgccggtgcg
      721 gttcacaccg cgcgcgaagg ccgcggcctg aacgacgacc tacccgggat cgacccgtcc
      781 cgcgtcaccg cgtggctccg tgagcacgtc gagggcctcg agccgccggt ccagtactca
      841 ctcatccctg ctggcggttc gaacctgacg taccgcgtcg ttgacaccgc cgggcatcag
      901 tgggcactcc ggcggcctcc ggtgaccgcg gtcctcgcca ctgcgcacga cgtcgaccgg
      961 gaatggagga tcatcgaggc cctcgggcga cacaccgacg tgccggtacc gccggcggtg
     1021 gcccgctgta ccgaccccga cgtgtccgac ggacccttct acgtgatggg cttcgtcgac
     1081 ggccctgtgc tgcgcgccca tgccgacgcc gccgcgctca gcgccgacgc cgcccagacc
     1141 gccaccgatt cgctcatcga tgtccaagtg gcgttccact cggtcgaccc cgacgagatc
     1201 ggactcgggg acctgtcccg gcaccgcacc ggctacgtgg aacgtcagct tcatcggtgg
     1261 cgcacccagg tcgagcgcgc ccaggtccga cccctcccgc tcctcgacga gctccacgac
     1321 cggctcgctc gcaccatccc cgtcgagcga gccgccccgg gactcgcgca cggcgactac
     1381 cggttcgaca acaccatcct cggccccgac caccgtgtcc acgccgtgct cgactgggag
     1441 ctgtgcacca tcggcgaccc cgtcgccgac tgctgctgga gcctgctgta ttggagcgac
     1501 ccgggagacc cgtcgccgtt cctccccgac gcaccgactc tggcggccac gttcccacgc
     1561 cgcgacgagg tggctgatcg ctacgcgaca cgctccggac acgacctgga caccttccca
     1621 tggttcaccg tcttcggtta ctggaagatg gcgtgcatcg tcgagggcgt ctacacccgg
     1681 cggctgcaag gcagccggag cggtaccggc acccaggacc tcggctcgat cgcgcgccga
     1741 gtcgagtcgc tgctcgatca cgcggccgag gtcgcgacca cggtctgtta gaccagactg
     1801 agccgagacg catcgggagg tttcgtggat cgcagatcgg tcgtcatcgt cggaacggtc
     1861 tccctcgcgc tcctcgtcgc gatcggcctc ggtatcgccc tcaccagcgg cgggagcggg
     1921 aaccagtcgg tgcgcacgcc accgggctcg gtgacgaccc tcccgatccc cgcgacgctg
     1981 ccaaccactt cgaccatcaa gccagtgatc accgccccgg ccaacacggt gccgcccccg
     2041 ccgaagccca ccgtggtcat cggtccctcc cccaccaccg cggcaccggc accggcaccg
     2101 gcaccggcac cgacgccgac caccagcccg agcggcggaa atgacactgg gatcaccgac
     2161 cacgagatcc gtctcactgc gatcgctgac accgcaccgg tcctccgggg cgtcgaggcg
     2221 tgggcgtcgg cgctgaaccg cgccggcggt cttgccaagc gcacggtgca cgtcgacgcg
     2281 cggctcgtcg cgacgcccgc cgactacagc gccgccgtct ccagcgcctg cgcgaacagc
     2341 ttcgcgatcg tcgggtcgag ctcgaccttc gacagccaga gcgccgggct gcagtgcggg
     2401 atccccgaga tcgcgacccg gctgttcgac ccggtccacc agtccctccg caacgcctac
     2461 gcggtcatcc cgactcgcgc gaacctcgag cgagtcggcg cgttcaaacg gctcttgagc
     2521 accgtcagcg gatgctgccg ccagtctgtg ctcgtcccga ccaccgagcc tggccgcacg
     2581 gcgacgacgc ggtccctgcg gggggcggag accatcgggt tcacgaccgc cgccacaccc
     2641 gatgtcagcc ccacggcgac ccccgccgac tacaccgcga tcgtcgctga cctcgtcgcc
     2701 aagcaggcca cgttcgcccg cagcgggctg ggagccgcgt cgaccgtgca gctccgcaag
     2761 gccgccgcgg ccaacagcgg ggcgagcacc gtgaaggcgt ggtactgcga cgccagctgt
     2821 gacgacccga cgttcctcac cgcaggcgga gccgcggtcg agggccagct catcgatgtc
     2881 ggcgtgaacc cgctcgtcga ccagcacgcc atccccgcga tggccacctt cgtgcgggcc
     2941 atgcgccatg tcggggcgac cccctcggtg ccggcgctcg agtcgtactc ggcgagcctg
     3001 ctgttcgagc aggcagcccg gcaggtgatc caggcgagcg gcacgaacgg actcacccgg
     3061 gtgcggctgc tcggagccgt cggcaccatg cacgccttca acgccggcgg gatcctcggc
     3121 gccaccgatg tcgccgcccg ccagccgaac ggctgcttca tgctggtgac cgtcacgaac
     3181 ggccacttcg tccgctcgta cccagccgca ccgggccagc tggactgtgg ggcccagaac
     3241 ctgcaaacgg ggagcggatg acaccgtttc gctacgacga tcagcgagtg gtgatcaccg
     3301 gcgcgtcgtc gggcatgggt gccgcgacgg ccgcgatgct cgtcgaggct ggcgccgagg
     3361 tccatgccct cgacatccgc gacgtgtcga cgccggtctc gaagtcctac gagaccgatg
     3421 tcggcgatcc cgacgccgtc aacacggcgc tggccgcgat cggggcaccg atccacaagc
     3481 tgttcaacgt ggccggcgtc ccccagaccc atccccccga acgggtcttc accgtgaact
     3541 tcctcgggtt gcgccacatg accgagcggg cgctcgacct gatgccggac gggggcgcga
     3601 tcgccaacgt cgcgtcgctc gccgggatgg gctggtcgga gcacgtgacc ccgatcggtg
     3661 agctgctcga cacccccgac gtcgccgccg gccaggcgtg ggccgcggcg catctcgccg
     3721 agtacggcga cccgtacttc ttctccaagg aatgtgtgat cgtctacacc ttccggctcg
     3781 cgccgcgact ggtccgcgag cgcgagatcc gggtcaactg catcagtccg ggcccggtcg
     3841 attcaccgat gatggtcgac ttccgcgccg cgctgtcatc ggccacgatc gactggaccg
     3901 cggcgcaagc caacggccgc ctcggccgtc ccgaagagat ggcgccaccc ctcgtcttct
     3961 tgaacagcga cgacgccagc tacgtcaacg gccacaatct ggtcgtcgac gccggcttca
     4021 cctcggccat gacgacg
//
LOCUS       DATCCH010000233         2523 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_344832, whole genome shotgun sequence.
ACCESSION   DATCCH010000233 DATCCH010000000
VERSION     DATCCH010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344832"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1030
                     /locus_tag="VIJ44_04605"
     CDS             <1..1030
                     /locus_tag="VIJ44_04605"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5165212.1"
                     /translation="RQLALLDDLEGRAAEPEELATLFELDQLATRMRRNAESLLVLAG
                     SDQPRQWRTAIPIIDVVRAAAAEIADFARVNYFGFGDDLAVAGHAVADVTHLLAELLE
                     NATSFSPPTAPVIVAGGPNDHRYVVTVTDEGIGIDDERLRQANALLVRPPAPGLELSR
                     TLGLHVVAHLAARHGIHIQLRPATATGVTAVIALPATILTRVAETPSPPAATAPPEPG
                     APFKLEPVPGPPPSVPATSWTAPAPTEPLSPTPAGAAPANGGEPDTANDPDGALPRRV
                     PTGATTRHDSGLVARVPGTHLSHRPPPDGPAPGETRPRPDRVQDLLTRHARGVRDGQN
                     PPNRDRER"
     gene            1027..1449
                     /locus_tag="VIJ44_04610"
     CDS             1027..1449
                     /locus_tag="VIJ44_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013875055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEY5165213.1"
                     /translation="MIRLSAEARNLNWLVSNFVAGVPGVTQAAVVSSDGLLIAMSNDL
                     DRVDADRLSAVAAGMQSIARGAAGSLHGGAVHEVIIEFEHALLFVMSVSETAVLAVAA
                     RRPCDAGLIGYEMAVLVERCATALSPALVAELQTALPH"
     gene            1458..1826
                     /locus_tag="VIJ44_04615"
     CDS             1458..1826
                     /locus_tag="VIJ44_04615"
                     /inference="COORDINATES: protein motif:HMM:NF017170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF742 domain-containing protein"
                     /protein_id="HEY5165214.1"
                     /translation="MSIGGPEPRFPRVVRPYVLTGGRTRPTGPTLALDTTIRTTVTAV
                     SVPSRPDTPEAARIVELCHTPTSLAELAGRLTLPVGVVRVLVGDLAATGTLTVDPATP
                     LGVATDVALLEKLLDGIRAL"
     gene            1807..2400
                     /locus_tag="VIJ44_04620"
     CDS             1807..2400
                     /locus_tag="VIJ44_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019889424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HEY5165215.1"
                     /translation="MASARFEPLRSWHAPVSMKIVVAGGFAVGKTTFVGAISEIEPLR
                     TEAEMTVESLGLDHAGPLADTKRGTTVAMDFGRITIDPTMVLYLFGTPGQDRFWFLWD
                     ELVRGAVGGVVLVDTRRLDDCFPAVDYFEHLGVPFVVAVNLFDGVATHHPREVREALS
                     VPANVPVVLCDARRRSHVQETLVLLVEAALRRVNGAA"
BASE COUNT          348 a         1118 c          712 g          345 t
ORIGIN      
        1 ccgccagctc gcccttctcg acgacctcga aggccgcgcc gccgaacccg aggagctcgc
       61 caccctcttc gagctcgacc agctcgccac ccggatgcgc cgcaacgccg agagcctcct
      121 cgtcctcgcc ggttccgacc agccccgcca atggcgcacc gcgatcccca tcatcgacgt
      181 cgtccgcgcc gcggccgccg agatcgccga cttcgcccgg gtcaactact tcggcttcgg
      241 cgacgacctc gccgtcgccg gccacgccgt cgctgacgtc acccacctgc tcgccgagct
      301 cctcgagaat gccacctcgt tctcgccacc gaccgcgccg gtcatcgtcg ccggcgggcc
      361 caacgaccac cgctatgtcg tcaccgtcac cgacgaaggc atcgggatcg acgacgaacg
      421 cctccgccag gccaacgcgc tgctggtccg gccccctgcg cctgggctcg aactctcccg
      481 gacgctcggt ctgcacgtcg tcgcgcacct cgcggcccgc cacggcatcc acatccagct
      541 ccggcccgcc accgccaccg gcgtcaccgc cgtcatcgcc ctccccgcca ccatcctcac
      601 ccgcgtcgcc gagactccct ccccgcccgc cgcgacggct ccacccgagc ccggggcgcc
      661 cttcaagctc gaaccagtcc cggggcctcc cccgtccgtc ccggcgacct cctggaccgc
      721 acccgcaccc accgagccgc tcagcccgac acctgccggc gcggcccccg ccaacggtgg
      781 cgaacccgac accgccaacg accctgacgg agcgctcccg cgccgagtcc ccaccggcgc
      841 cacgacccgg cacgacagcg ggctcgtcgc ccgcgtccct gggacccatc tctcgcaccg
      901 gccaccaccc gacggacctg cacccggcga gacccggccc cgtcccgacc gggtccagga
      961 cctcctcacc cgacacgccc gcggcgtgcg agacggccag aaccccccga accgggaccg
     1021 agagcggtga tccgactcag cgccgaggcc cgcaacctca actggctcgt ctccaacttc
     1081 gtcgccggcg tcccgggcgt cacccaagcc gcggtcgtgt cctccgacgg gttgctcatc
     1141 gccatgtcca acgacctcga ccgcgtcgac gccgaccggc tctccgccgt cgccgccggg
     1201 atgcaaagca tcgctcgggg tgccgccggt tcccttcacg gcggcgcggt ccacgaggtc
     1261 atcatcgaat tcgagcacgc cctcctcttc gtgatgagcg tcagcgaaac cgcggtgctc
     1321 gccgtcgcgg cccggcgacc ctgcgacgcc ggcctgatcg gctacgagat ggctgtgctg
     1381 gtcgaacggt gcgccaccgc cctcagcccc gcgctcgtcg ccgaactcca aaccgcgctc
     1441 ccgcactaaa ggacccgatg agcatcggtg gccccgagcc tcgcttcccc cgagtggtcc
     1501 gcccctacgt cctcaccggc ggccgcaccc gacccaccgg accgaccctg gccctcgaca
     1561 ccaccatccg caccaccgtc accgcggtca gcgtgccctc gcgtcccgac acccccgaag
     1621 ccgcccgcat cgtggagctc tgccacacac cgacctccct cgcggagctc gccggccgtc
     1681 tcaccctccc cgtcggcgtc gtccgggtcc tcgtcggcga cctcgcggcc accggcaccc
     1741 tcaccgtcga tcccgccacc cctctcggcg tcgccaccga cgtcgccctc ctcgagaagc
     1801 tgctcgatgg catccgcgcg ctttgaaccc ctgcgttcgt ggcacgcccc ggtctcgatg
     1861 aagatcgtcg tcgccggcgg gttcgcggtc ggcaagacca ccttcgtcgg cgcgatctca
     1921 gagatcgaac cgctgcgcac cgaagccgag atgaccgtcg aaagcctcgg cctcgaccat
     1981 gccggaccgc tcgccgacac caagcgcggc acgaccgtcg ccatggactt cgggcgcatc
     2041 accatcgacc cgacgatggt cctctacctg ttcggcaccc ccggccagga ccgcttctgg
     2101 ttcctctggg acgagctggt ccggggcgcg gtcggcggag tcgtcctcgt cgacacccgc
     2161 cgccttgacg actgcttccc cgcggttgac tacttcgagc atctcggcgt gcccttcgtc
     2221 gtcgccgtca acctcttcga cggggtcgcc acccatcatc cccgcgaggt ccgagaggcg
     2281 ctctcggtcc ccgccaacgt ccccgtcgtg ctctgcgacg cccgccgccg cagccacgtc
     2341 caagagaccc tggtactcct cgtcgaagcc gcgctgcggc gcgtcaacgg cgccgcctga
     2401 cccgcccgtc ggatcagccc tcgcatcgga acccggcggc ggtgcccccg ccggtccacc
     2461 gtcgtgtccc gggcggggca cccgccgtcg tgcgcaggac agcgccattg ctgccacttt
     2521 gcg
//
LOCUS       DATCCH010000234         4576 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_53927, whole genome shotgun sequence.
ACCESSION   DATCCH010000234 DATCCH010000000
VERSION     DATCCH010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4576
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53927"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2197
                     /locus_tag="VIJ44_04625"
     CDS             <1..2197
                     /locus_tag="VIJ44_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEY5165216.1"
                     /translation="TRGQIVVVDGTRAGTLEGVTRMAYDGTKLPVRDAELHEAQITTR
                     DIDRGEYPHYLLKEISEAPNSFRKTLRGKIIETPAGDEDANREPRLRVELGAEALPDS
                     LVAALRSGQIRRVAVIGQGTAAVAGQSLALALRDAVEDRLIVDAVHATELSGFGLRED
                     MADTLVVAISQSGTTTDTNRTVDLARERGATVVAIVNRRNSDLVDKADGVLYTSDGRD
                     VEMSVASTKAFYAQVAAGYLFALGIASEVGVAPARSERVLGALREIPDAMRSVINDRE
                     AIAGVAQRHVLNRRYWAVVGNGRNVVAARELRIKLSELCYKAIACDVTEDKKHIDLSS
                     EPLVLVCAAGLGGSNVDDVAKEVAIYGAHRAAPLVVASEDDAPRFAHALEVIPVPAVH
                     PALDFVLSTVAGHLFCYEAALAIDGSARPLREARAAIEAAAPGPPPTLLDRLAPRLEA
                     PARRFVDGLRSGQYDGTLEASTATRVASLLRYSAGTLPLDSYELEHGSVGTPSVVVQD
                     LTVALTKAIEELTRPIDAIKHQAKTVTVGISRSDETLLHVPLVREVIAAGAPRDGLAF
                     RALRTLAELDPAVTTVTGYTRYRIEGEVDGDTATVHVIDRGGVSASLASRTDADPRLR
                     GTKHRVANQREVTAVRGRADGRTIVMVPEVKNNQTVGLTLLHVQFADLLPPDAMRAVL
                     QGYQGRYAALKDAVTETEPTFDDTTLGSIPVVDLLTEPVYVLAERWRRG"
     gene            2245..2637
                     /locus_tag="VIJ44_04630"
     CDS             2245..2637
                     /locus_tag="VIJ44_04630"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEY5165217.1"
                     /translation="MRDATLNIVGLGTDLVEIPRFRLAMERRGQRLDERLFSDDERAY
                     AYRQKDPAKSLAARFGAKEAVMKALGVGLWKFKLRDVEVVRQRSGAPRVALHGRAQEL
                     ASERGVRAWHLSLTHTDTTALAVAIAVG"
     gene            2646..4082
                     /locus_tag="VIJ44_04635"
     CDS             2646..4082
                     /locus_tag="VIJ44_04635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEY5165218.1"
                     /translation="MEPVVTPQEMGEADRRTIAAGTPVEALMERAGRSVAWGVRRRLD
                     GVYGLRAVVVCGKGNNGGDGLVAARVLAGWGVRVDVAQLADPPDAAALGRMLDRADVL
                     VDAMYGTGFTGTLDGAAAAVAARSVDLPTVAVDIPSGVDGQTGATSGMAVRAAATVTF
                     AARKPGLVFEPGRSHAGVVQVADIGIELGPVSLGLTEERDARGWLLAHERPPNTNKWQ
                     AGVLVVGGSDGMTGAPHMVSEAAMRAGAGIIWCGVPGHEAATRAGGSEIITKALPATD
                     DGALAGLDDLLSSIDRFRALAVGPGLGTADPTRAAVRALVAKVTVPMVLDADGLNVFA
                     GDAAKLRERPGPTVLTPHEAEYARLAGEPVGADRVDAARRLATLSGAIVLLKGPATVI
                     AEPGGRAVLNPTGGPELATAGSGDVLSGIIAGLIARGLSPFEAAAAGAFIHGRAGDVS
                     GHTGLIASDLIVALPRTLTDLTSSPREP"
     gene            4113..4553
                     /locus_tag="VIJ44_04640"
     CDS             4113..4553
                     /locus_tag="VIJ44_04640"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY5165219.1"
                     /translation="MTKNVVTLQPDQTFEVAADLLAEKGIGAAPVVDGDRVVGVLRDE
                     DLIVSEANLHVPTWFNFGLGVEFPLPGQAKRFEGELKRMVASTVRDLMTTTFESCGPD
                     DTLGVVAAKMHDRDVTHMPVIDADHHLVGIIARGDLVRRVAAET"
BASE COUNT          711 a         1681 c         1534 g          650 t
ORIGIN      
        1 gacccgcggg cagatcgtcg tcgtcgacgg aacccgggcc ggaaccctcg agggcgtcac
       61 ccgaatggcc tacgacggga ccaagcttcc cgttcgcgat gccgagctgc acgaggccca
      121 gatcaccacc cgagacatcg accgcggcga gtacccgcac tacctcctca aagagatctc
      181 cgaggccccg aactcgtttc gaaagaccct gcggggcaag atcatcgaga ccccggcggg
      241 ggacgaagac gcgaaccggg aaccccgact gcgagtcgag ctgggggccg aggccctgcc
      301 cgactcactc gtcgctgccc tgcgaagcgg ccagattcgc cgagtggcgg tcatcggcca
      361 aggcaccgcg gcggtggccg gtcagagtct cgcgctcgcg ctccgcgacg cggtcgagga
      421 ccggctgatc gtcgacgctg tccacgcgac cgagctgtcc ggcttcggtc tccgcgagga
      481 catggcggac acgctcgtgg tcgcgatcag ccagagcggc accaccaccg acaccaaccg
      541 caccgtcgat ctggcccgcg aacgcggcgc gaccgtggtc gcgatcgtga accggcgcaa
      601 cagtgacctg gtcgacaagg ccgacggtgt cctgtacacc tccgacgggc gcgatgtcga
      661 gatgagtgtc gcctcgacga aggcgttcta cgctcaggtc gccgcgggct acctgttcgc
      721 tcttggcatc gcaagcgagg tcggcgtcgc accggctcgc agcgaacggg tgctcggcgc
      781 gctgcgcgaa attccggatg ccatgcggtc ggtgatcaac gaccgtgaag ccatcgctgg
      841 tgtcgcgcag cgccacgtgc tgaaccgtcg ttactgggcc gtcgtgggca acggacgcaa
      901 cgtcgtcgcg gcccgagagt tgcggatcaa gctctcggaa ctgtgttaca aggccatcgc
      961 ctgcgatgtg accgaagaca agaagcacat cgatctgtcc tccgagccgc tcgtcctcgt
     1021 ctgcgcggcc gggctcgggg ggtcgaatgt cgacgatgtg gcgaaggaag tcgcgatcta
     1081 cggtgcccac cgtgccgctc cgctggtggt ggccagtgag gacgacgcgc ctcgcttcgc
     1141 tcatgccctc gaggtcatcc cggttcccgc ggtgcacccg gccctcgact ttgtgctctc
     1201 gaccgtggcg ggacacctgt tctgctacga agccgccctc gcgatcgacg gttcggcacg
     1261 cccgctgcgg gaggcacggg ccgcgatcga ggccgcggca ccgggaccgc ccccaacgct
     1321 cctcgatcga ctcgcgcccc gtcttgaggc accagcgcgc cgtttcgtcg acggtctccg
     1381 ctccggccag tacgacggaa ccctcgaggc cagcaccgcc acccgagtcg cctccctcct
     1441 ccggtattcc gccgggacgc tcccgctcga ctcctacgag ttggagcacg gcagcgtcgg
     1501 caccccgagc gtcgtcgtcc aagacctcac cgtggcgctc accaaagcga ttgaagagct
     1561 cacccggccc atcgatgcca tcaagcacca ggccaagacc gtcacggttg ggatctcacg
     1621 atccgacgag accctgctcc acgtccccct cgtccgcgag gtgatcgccg cgggcgcacc
     1681 ccgcgacggt ctggcattcc gggcgctgcg tacgctcgcc gagctcgatc ccgccgtcac
     1741 gacagtcacc ggctacaccc gctaccgaat cgagggcgaa gtcgacgggg acaccgcgac
     1801 ggtgcacgtc atcgaccgcg gtggggtctc agcatcgttg gcgtcgcgca ccgacgccga
     1861 cccccgcctc cggggcacga agcaccgggt cgcgaaccag cgtgaggtca ccgcggtccg
     1921 cgggcgcgcc gacggtcgca cgatcgtgat ggtccccgaa gtgaagaaca accagacggt
     1981 cgggctcacc ctcttgcacg tccagttcgc cgacctgctc cccccggacg cgatgcgagc
     2041 cgtcctccag ggataccaag gccgctacgc agcgctcaag gacgccgtca ccgagaccga
     2101 gccgacgttc gacgacacca cgctgggctc gatcccggtg gtcgacctcc tcaccgaacc
     2161 ggtctacgtc ctcgccgagc gctggcgccg agggtgaacg aaccctcgcc ggccgggccg
     2221 accggcgacg cgtctggacc agaggtgcgg gacgccacgc tcaacatcgt cgggctcggc
     2281 accgacctcg tcgagatccc ccgcttccgt ctcgccatgg agcgtcgagg gcaacgactc
     2341 gacgagcgac tcttcagcga cgacgagcgg gcgtacgcct accgtcagaa ggacccggcc
     2401 aagagcctcg ccgcccgatt cggggcgaaa gaagctgtca tgaaagccct cggggtcggt
     2461 ctctggaagt tcaagctccg cgacgtcgaa gttgtccgcc agcgcagcgg cgcaccccgg
     2521 gtcgcgctcc acggccgcgc gcaagagctc gccagcgaac gcggcgtgcg agcctggcac
     2581 ctgtcgctca cccacaccga caccaccgcc ctcgccgtgg ccatcgccgt cgggtaggcg
     2641 cgagcatgga gccggtcgtc acaccccagg agatgggcga ggctgaccgt cgcaccatcg
     2701 cggccgggac ccccgtcgag gcgctcatgg aacgcgccgg gcgatcggtg gcgtgggggg
     2761 tccggcgccg cctcgacggc gtctacggtc tccgggccgt cgtcgtgtgc gggaagggca
     2821 acaacggcgg ggacggcctc gtcgcggccc gggtactcgc gggctgggga gtccgggtcg
     2881 acgtcgcgca actcgctgac ccccccgacg ccgcggcgct cggtcggatg ctcgaccgcg
     2941 ccgatgtcct cgtcgacgcc atgtacggga cgggcttcac cggcaccctc gatggcgccg
     3001 ccgccgccgt cgcggcgcgc agcgtcgacc taccgacggt cgccgtcgac atcccatccg
     3061 gggtcgacgg ccagaccggc gccacgtcgg gaatggcggt ccgtgcggcg gccacggtca
     3121 cgttcgccgc ccgaaagccc ggtctggtgt ttgagcccgg ccgctcccac gccggggtcg
     3181 tgcaggtcgc tgacatcggc atcgagctcg gcccagtcag cctcgggctc accgaggagc
     3241 gcgacgcccg tggctggctc ctcgcccatg agcgtccgcc gaacaccaac aaatggcagg
     3301 ccggggtgct cgtcgtcggt ggctccgacg ggatgaccgg ggcgccacac atggtcagcg
     3361 aggccgcgat gcgcgccggc gcgggcatca tctggtgcgg ggttcccggt catgaggctg
     3421 ccactcgcgc cggtggctcc gagatcatca ccaaggccct ccccgcgact gacgacgggg
     3481 cgctcgccgg actcgacgac ctgctgtcga gcatcgaccg cttccgggcg ctcgccgtgg
     3541 gtcccgggct cgggacggcc gacccgaccc gcgccgcggt ccgcgcgctg gtggccaagg
     3601 tgacggtccc gatggtgctc gacgccgatg ggctcaacgt cttcgccggt gatgccgcca
     3661 agcttcgtga gcgtcccgga ccgaccgtgc tcacccccca cgaggcggag tacgcacggc
     3721 tcgccggtga gcccgtcggg gctgaccggg tcgacgcggc gcggcggctg gccacgctca
     3781 gcggggcgat cgtgctgttg aagggcccag ccaccgtcat cgccgagccc ggcggccgcg
     3841 ccgtcctgaa cccgaccggc ggccccgagc tcgcgaccgc cgggagcggc gacgtcctct
     3901 ccgggatcat cgccggcctg atcgcccgag ggctgtcccc cttcgaggcc gcggccgcag
     3961 gcgccttcat ccacggtcgc gccggtgacg tctccgggca tactggactc atcgccagtg
     4021 acctgatcgt ggccctgccg cgcacgctca ccgacctgac ctcaagcccc cgggagccgt
     4081 aaatgccagc gtcaacccct gtccgagatg tcatgacgaa gaacgtcgtg acgctccaac
     4141 cggatcagac gtttgaggtc gcggcagacc tcttggccga gaaggggatc ggcgccgcgc
     4201 cggtcgtcga cggcgaccga gtggtcggtg tgcttcgcga cgaggacctc atcgtctccg
     4261 aagccaacct ccatgtcccc acgtggttca acttcggcct cggtgtggag ttccccctcc
     4321 ccggccaggc caagcgcttc gagggagagt tgaagcgcat ggtggcctcg acggtacggg
     4381 atctcatgac caccacgttc gaatcgtgcg gaccggacga cacgctcggc gtcgtcgcag
     4441 ccaagatgca cgaccgcgac gtgacgcaca tgccggtcat cgacgccgat catcacctgg
     4501 tcgggatcat cgcccggggt gacctggtgc ggcgagtcgc cgcggagacg tgaccggcgg
     4561 cgcgccgcac ttccgg
//
LOCUS       DATCCH010000235         4035 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_564501, whole genome shotgun sequence.
ACCESSION   DATCCH010000235 DATCCH010000000
VERSION     DATCCH010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4035
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564501"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1130
                     /locus_tag="VIJ44_04645"
     CDS             <1..1130
                     /locus_tag="VIJ44_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020557805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEY5165220.1"
                     /translation="ARLGADVIKVEHPRDGESGRGALPAMTDPTGRLAGATFLRNNLN
                     KRSVGIDLKAPEGRDLFRALAGHVDIVAENFKPGTMARMGLAYEDLAPQYPRLIYVSV
                     SGFGNTVPSPYDSWAAYAPIVEAMSGIYAFKQLEDQPPVVGPVGALGDISSAMFAVIG
                     TLAALRHRDHTGEGQYVDVAMLDAVVAMTDLVTNFWSMGLRPGGLGPLLIMDGFRARD
                     GWFVVQVGREHQFERLAKMIGRPEWLDDPRFASREGWRIHLEEVIRPAIEGWAAAKTK
                     LEAARELNEAGIAAGPVNSAPDVIADPHVAARDMLVEVPRTDGEPPYLMPGNPVKMTK
                     VAEGPETRVPWVGEHTDEILSQELGLTEADLTRLRDAGVIG"
     gene            1223..2560
                     /locus_tag="VIJ44_04650"
     CDS             1223..2560
                     /locus_tag="VIJ44_04650"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEY5165221.1"
                     /translation="MTLPRVTDLRRRARRQRRWGFASVIRDQHRMWWLVVLVGLGSGL
                     VGAGYVELLKLLTSVLGPTGFGRPEHLLILTAVGLTIGVLTLTLGNPGDVELLVDNIH
                     VTGGRSDVRELRSLIPVSLLGIAAGSAIGPEAPLVQTTGAIGTWLARHRRLDVSDTRV
                     LTITGMAAGFTVLFGAPFGSAIFALEILHRRGLQYYEALLPAAIGSLSGYVVYAGVTG
                     FGFHPIWTFPEPHGLRPVDFAVGLGAGVVGAVIAIAFTWCSKVLRRAFRRLPAVVRPV
                     VGGAALGGLAFLSPYALTFGEGQVQTVATAKLAVATLLVAVFAKLVASSMIVSSGWRG
                     GFIIPLFFVGVALGSIAHELLGVDRLVAMAAAMVAVNVGVTKTPFGSSVVVAEMAGVR
                     LLPPVLLASIVALFLTNRTSMIDTQREREEVSASDPSGHRPFGSLPEAEGGPD"
     gene            2697..2903
                     /locus_tag="VIJ44_04655"
     CDS             2697..2903
                     /locus_tag="VIJ44_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165222.1"
                     /translation="MNLLALAHQTGVRLAEIGFLLVLFAGVWLAAAQVPKFKLGTART
                     IVAGVALALAGLLLIIATHWGHFG"
     gene            complement(2900..3763)
                     /locus_tag="VIJ44_04660"
     CDS             complement(2900..3763)
                     /locus_tag="VIJ44_04660"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY5165223.1"
                     /translation="MPPLSTMVRVGAQRVACHDWGGTGDPVVLAHATGFHGLVWRPVA
                     ERLVAAGRRVWSFDFRGHGDSDRSTDGYAWARFGDDAAGVLAALGVEGDPGLLTVGHS
                     KGAAALLLAEVARPGTIRRAWCYEPIIIPGEAHDPDPDFPLAVGARRRRSQWDTPEEA
                     YRSWAARPPFAALHPDALAAYVEHGLRRRTDGTWELKCAAEDEAATYAHGPSHGLWSA
                     LPTIHSTVRVVCGERSDAVPPRLAARIAARLEHGSVEVLPGVSHFGPLEDPDAAVASI
                     LAFDDATAIPI"
     gene            3784..>4035
                     /locus_tag="VIJ44_04665"
     CDS             3784..>4035
                     /locus_tag="VIJ44_04665"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY5165224.1"
                     /translation="MAAEDEVRAANGAFYSAFESGDLDAMAEVWDRSDRARVTHPGWP
                     TLYGWAKVLGSWEAIFANTEFIQFVLTEERMLVVDDVAIV"
BASE COUNT          573 a         1418 c         1409 g          635 t
ORIGIN      
        1 tggcccgcct cggcgccgac gtcatcaagg tggagcatcc ccgcgacggc gagtcggggc
       61 gcggggccct cccggcgatg accgacccga ccggtcgcct cgccggcgcg acgttcctgc
      121 gcaacaacct gaacaagcgc agcgtcggga tcgacctgaa ggcccccgag ggccgagacc
      181 tgttccgcgc cctggctggc cacgtcgaca tcgtggccga gaacttcaaa cccgggacca
      241 tggcccgcat gggtctcgcc tacgaggacc tggcccccca atacccgcgc ctgatctacg
      301 tctcggtgtc gggcttcggg aacacggtgc cgtcgcccta tgactcgtgg gccgcgtacg
      361 cgccgatcgt ggaggcgatg tcgggcatct acgcgttcaa gcagcttgag gaccagccac
      421 cggtcgtcgg acccgtcggc gcgctcggcg acatctcgag cgccatgttc gcggtcatcg
      481 gcaccttggc cgcgctccgc caccgcgacc acaccggcga aggccagtac gtcgacgtcg
      541 cgatgctcga cgcggtggtg gccatgaccg acctcgtcac gaacttctgg tccatgggcc
      601 tgcgccccgg ggggctcggg cccttgctca tcatggacgg cttccgggcc cgcgacggct
      661 ggttcgtggt gcaggtcgga cgagagcacc agttcgagcg gctggcgaag atgatcggcc
      721 ggcccgagtg gctcgacgat ccgcgcttcg cctcgcgtga gggatggcgc atccacctcg
      781 aggaggtgat ccgacccgcc atcgagggat gggccgccgc caaaaccaaa ctcgaggcgg
      841 cgcgggagct gaacgaggcc gggatcgcgg ccgggccggt gaactcggct cccgacgtca
      901 tcgccgaccc gcacgtcgcg gcccgcgaca tgctcgtcga ggtgccgcgc accgacgggg
      961 agcccccgta cctcatgccg ggcaacccgg tgaagatgac caaggtcgcc gaaggccccg
     1021 agacccgagt cccgtgggtc ggcgagcaca ccgacgagat cctgagccaa gagctcgggc
     1081 tcaccgaagc cgacctcacc cgactgcgag acgcgggcgt catcggctga accccgagtc
     1141 cccccggtcg acgctggcga gcgacccgtc acccgccgga cgcgggtccg tccgaccgat
     1201 ccaaagaata tcctattacg atatgacgct gccgcgggtg accgaccttc gccgccgcgc
     1261 ccgacgccag cgacgctggg gcttcgcgtc ggtgattcga gaccaacacc ggatgtggtg
     1321 gctcgtggtg ctcgtcgggc tgggctcggg tctggtcggc gcgggatacg tcgagctgct
     1381 caagctcctc acgagcgtgt tggggccgac cggattcggc cgccccgagc atctgctgat
     1441 cctcaccgcc gtcggcctca cgatcggagt cctcacgttg acactcggca accccggcga
     1501 cgtggagctg ctggtggaca acatccacgt caccggtggg cgctccgatg tccgggagct
     1561 gcgctcactg atccctgtgt cgctgctcgg catcgccgcg gggagcgcca tcggccccga
     1621 ggcgccgctc gtgcagacca ccggcgcgat cggcacctgg ctcgctcggc accgccgcct
     1681 cgacgtctcc gacacccggg tgctcacgat caccgggatg gctgccgggt tcacggtgct
     1741 gttcggcgct ccgttcggtt cggcgatctt cgcgttggag atcctgcacc gtcgggggct
     1801 gcagtactac gaggcgttgc tgccggccgc catcggatcg ctctcgggct acgtcgtgta
     1861 cgccggtgtc accgggttcg ggttccatcc gatctggacg tttcccgagc cgcacggcct
     1921 ccgaccggtg gacttcgcgg tgggcctcgg cgccggcgtg gtcggcgccg tcatcgcgat
     1981 cgcgttcacc tggtgctcca aggtcctgcg ccgtgccttc cgtcgcctgc cggcggtggt
     2041 ccgaccagtg gtcggaggag ccgcgctcgg cggcctggcg ttcctgagcc cctacgcgct
     2101 gacgttcggt gagggacagg ttcagacggt cgcgaccgcg aagctcgcgg tcgcgacgct
     2161 cctggtcgcc gtgttcgcca agctcgtggc gtcgtcgatg atcgtgtcgt cgggatggcg
     2221 aggcgggttc atcatcccgc tgttcttcgt tggcgtagcg ctcggctcca tcgcccacga
     2281 gctcctcggt gtcgaccgcc tcgtcgccat ggccgcggcg atggtggccg tcaacgtcgg
     2341 cgttaccaag acgccgttcg gatccagcgt ggtggtcgcc gagatggcag gtgtgcgcct
     2401 gctcccgccg gttttgctcg cgtcgatcgt cgcgctgttc ctgaccaatc ggacgtcgat
     2461 gatcgacacc caacgagaac gcgaagaggt gtcggcgtcg gacccgtctg ggcaccgacc
     2521 gttcggctcg ctcccggagg ccgagggggg tcccgactag tgccgtgtca ggcaacgttt
     2581 gctcggttcg gtaggggact cgctggccgc ataggcacgc cgaacggcag ttatgtattg
     2641 gcggcggcta cgccccggct cgacacgggt ggagcgaggc aggaagaatg ctgcgcatga
     2701 acctccttgc tctggcgcat cagacgggcg tgcgcctcgc ggaaatcgga ttcttgctcg
     2761 tcctgttcgc tggggtctgg ctggccgcgg cgcaagtccc gaagttcaaa cttggtaccg
     2821 ctcgaacgat cgtcgcaggg gtcgcgctcg cgctcgcggg tctacttctc atcatcgcca
     2881 cgcactgggg tcacttcggc tagatcggga tggcggtggc gtcgtcgaag gccaagatcg
     2941 acgccacggc ggcgtcggga tcctcgagcg gaccgaagtg gctaacgccg gggagcacct
     3001 cgacgctccc gtgctccaat ctcgccgcga tccgtgccgc gagccgcggc ggaaccgcgt
     3061 cggaccgctc gccgcacacc acccgcacgg tgctgtgaat cgtgggcagc gccgaccaga
     3121 gcccgtgcga tgggccgtgc gcgtacgtgg cggcctcatc ttcggcggcg cacttcagct
     3181 cccaggtccc gtcggtgcgg cgccgcaacc cgtgctcgac gtacgccgcc agggcgtcgg
     3241 ggtgcagggc cgcgaacggc ggccgggccg cccaggagcg gtacgcctcc tcgggcgtgt
     3301 cccactggga gcgtcgtcgt cgggcgccga ccgccaacgg gaagtcggga tcggggtcgt
     3361 gcgcttcgcc ggggatgatg atcggctcgt agcaccaggc ccggcggatc gtcccaggac
     3421 gcgcaacctc cgcgagcagg agcgcggcgg cgcccttcga gtggccgacg gtcaggagac
     3481 ccgggtcgcc ctccaccccc agcgcggcga ggacaccggc ggcgtcgtca ccgaagcgcg
     3541 cccaggcgta cccgtcggtc gaacggtcgc tgtcaccgtg acctcgaaag tcgaaggacc
     3601 agacgcgccg gcctgccgcg acgagccgct cggccaccgg gcgccacacc aggccgtgga
     3661 acccggtcgc gtgcgccagg accactgggt cgcccgtgcc accccagtcg tggcacgcca
     3721 cgcgttgcgc cccgacgcgg accatcgtcg acagcggagg cacggccagc catgatgctc
     3781 ggcgtggccg ccgaggacga agtgcgcgcc gcgaacggcg ccttctactc ggcgttcgag
     3841 agcggagacc tcgacgcgat ggccgaggtg tgggatcgct cggaccgggc ccgggtcacc
     3901 caccccgggt ggccgacgct gtacggatgg gcgaaggtgc tcggctcgtg ggaggcgatc
     3961 ttcgccaaca ccgagttcat ccagttcgtc ctcaccgagg aacggatgct cgtcgtcgac
     4021 gacgtggcga tcgtc
//
LOCUS       DATCCH010000236         2023 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_523945, whole genome shotgun sequence.
ACCESSION   DATCCH010000236 DATCCH010000000
VERSION     DATCCH010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523945"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..988)
                     /locus_tag="VIJ44_04670"
     CDS             complement(<1..988)
                     /locus_tag="VIJ44_04670"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY5165225.1"
                     /translation="MSAAALGGVLAVTTTWKLVLPVLMAASVLAFALALLLITGDHRS
                     RLERRLTGYGEPEPDPVERSVPRGEQMLAETRLVQDMAEFTGRMAERVGLLNRVEEKL
                     EQADLPIRPTEAIFFYGVGVFVVLLAALLLLGFPSGLAVALLAAIGPVAFLELRRKRR
                     LRKFETQLPDVLTLLAGSMRAGFSFAQGLEAVAAEAPEPSGRELQRCFTESRLGRPIE
                     DSMEDSANRMHSVDLMWAVMAIRIQREVGGNLAELLDTVATTMTERERLKHEMLALTA
                     EGRLSAWILGVFPPGFALVLFTIQPDYMKVLFQNGIGVMAVIVSAVMAGIGFF"
     gene            complement(995..>2023)
                     /locus_tag="VIJ44_04675"
     CDS             complement(995..>2023)
                     /locus_tag="VIJ44_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018769165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HEY5165226.1"
                     /translation="PDQVYIEREGKITRTDVRFVDEMHLRRIIDKIVSQVGRRVDEAT
                     PMVDARLPDGSRVNCIVHPLAIGGPFLTIRRFRADPFTVEDLVTMSTMSEQVAHFTEC
                     CVRGRLNVVVSGGTGTGKTTLLNVLSSFIPADERIVTIEDAKELQLQQPHVLCLEARP
                     PNIEGKGEIRIRDLVRNSLRMRPDRIVVGEARGAEALDMLQAMNTGHDGSLTTVHSNA
                     PRDTLSRIETMTLMAGMDLPVRVIREQMASALDLIVHLSRLRDGTRRVTYVSEVMGME
                     GDVIVLQDLYMFDYSMGIDSDGRFLGQLKSTGIRPGFSERLANYGVVLDHALFGGEAV
                     SRRQGSRR"
BASE COUNT          343 a          737 c          628 g          315 t
ORIGIN      
        1 agaagaaccc gatcccggcc atgaccgccg agacgatcac cgccatcacg ccgatcccgt
       61 tctggaacag caccttcatg tagtcgggct ggatcgtgaa caacacgagc gcgaacccag
      121 gcgggaacac tccgaggatc cacgccgaca gtcgcccctc cgcggtgagg gccagcatct
      181 cgtgcttgag ccgctcccgt tccgtcatcg tcgtggcgac ggtgtcgagc agctcagcca
      241 ggttgccgcc gacctctcgt tggatgcgga tcgccatgac cgcccacatc aggtccacgc
      301 tgtgcatccg gttcgccgag tcctccatcg aatcctcgat cggccggccg agacgactct
      361 cggtgaagca gcgctgcagc tcccggcccg acggctccgg cgcttccgcg gcgaccgcct
      421 cgagaccttg cgcgaatgag aacccggccc gcatcgaccc ggccaggagg gtcagcacat
      481 ctggcagctg ggtctcgaac ttgcgcagcc gtcgcttgcg acgcagctcg aggaacgcca
      541 ccggcccgat cgccgcgagc aacgccacgg ccagccccga cgggaacccg aggagcagca
      601 gcgccgcgag gaggaccacg aacacgccga cgccgtagaa gaagatcgcc tcggtcggac
      661 ggatcggcag gtcggcttgc tcgagcttct cctcgacccg gttcaacagg cccacccgct
      721 ccgccatccg gccggtgaac tccgccatgt cctgcaccaa gcgggtctcg gcgagcatct
      781 gctccccgcg gggcaccgac cgctctaccg ggtccggttc gggctccccg tacccggtga
      841 ggcgtcgctc gaggcggctg cggtgatcgc cggtaatgag cagcaacgcc aacgcgaacg
      901 ccagcaccga cgccgccatc agcaccggta gcaccagctt ccaggtggtg gtcaccgcaa
      961 gcaccccgcc caacgctgcc gcgctcatcg ccgcctaccg ccggcttccc tggcggcgcg
     1021 agaccgcctc gccgccgaac aacgcgtggt cgaggaccac cccgtagttc gcgagccgct
     1081 ccgagaaccc gggtcggatc ccggtgctct tcagctggcc gaggaaccgc ccgtcggagt
     1141 cgatccccat cgagtagtcg aacatgtaga ggtcttggag gacgatgacg tcgccttcca
     1201 tccccatcac ctcgctgacg tacgtcacgc gccgggtccc gtcccgcagc cgcgacaggt
     1261 gcacgatcag atccagcgcc gatgccatct gctctcggat gacccgcacc ggcaggtcca
     1321 ttccggccat gagcgtcatc gtctcgatcc gagacaacgt gtcacgcggc gcgttggagt
     1381 gcaccgtggt cagcgacccg tcgtgaccag tgttcatggc ctggagcatg tcgagcgcct
     1441 cggcacctcg cgcctcaccg acgacgatgc ggtccggccg catccgcaac gagttgcgca
     1501 ccaggtcccg gatccggatc tcacctttgc cttcgatgtt gggcggccgg gcttcgaggc
     1561 agagcacatg cggctgctgg agctgcagct ccttcgcgtc ctcgatcgtc acgatccgct
     1621 cgtcagcggg gatgaacgac gacaagacgt tcagcaacgt cgtcttccca gtaccggtcc
     1681 cgccgctcac caccacgttg agccgacctc gcacgcagca ctcggtgaag tgcgccacct
     1741 gctcgctcat cgtgctcatc gtcacgaggt cctcgaccgt gaacgggtcg gcccggaacc
     1801 gccggatggt gagaaacggg ccaccgatgg cgagggggtg gacgatgcag ttcacccgag
     1861 acccgtcggg gagccgggcg tcgaccatgg gggtcgcttc gtccacgcgg cgaccgacct
     1921 ggctgacgat cttgtcgatg atgcgccgca ggtgcatctc gtccacgaac cggacgtcgg
     1981 tgcgggtgat cttcccttcg cgttcgatgt acacctggtc ggg
//
LOCUS       DATCCH010000237         2654 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_12549, whole genome shotgun sequence.
ACCESSION   DATCCH010000237 DATCCH010000000
VERSION     DATCCH010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12549"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1269
                     /gene="leuC"
                     /locus_tag="VIJ44_04680"
     CDS             <1..1269
                     /gene="leuC"
                     /locus_tag="VIJ44_04680"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEY5165227.1"
                     /translation="RLSGRPVRRPDLTVATMDHNVPTTDIDRPIADPISAKQIAVLAA
                     NCAEFGVRLYKMGDPGQGIVHIIGPEQGLTLPGMTIVCGDSHTSTHGAFGALAFGIGT
                     SEVEHVLATQTLPQARPGTMAVTVEGDLPPGVTAKDVVLSIIARIGTAGGIGSIIEYR
                     GSAIRALSMEGRMTICNMSIEAGARAGLIAPDDTTFAYLEGRPFVPSGAAWERALDEW
                     RSLATDDDATFGRVVHLDAAELRPSVSWGTNPGQTVTIDDRVPDPDSFGEPHQRDAAR
                     LALRYMGLEAGTAMRDISVDTVFIGSCTNSRIEDLRSAAGVLEGRSVRDGLRALVVPG
                     SHRVKAQAEAEGLDRVFSSAGFEWREPGCSMCLAMNPDKLAPGERCASTSNRNFEGRQ
                     GKGGRTHLVSPAVAAATAVAGRFAAPEDLE"
     gene            1272..1886
                     /gene="leuD"
                     /locus_tag="VIJ44_04685"
     CDS             1272..1886
                     /gene="leuD"
                     /locus_tag="VIJ44_04685"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEY5165228.1"
                     /translation="MDPVREIVGRAVPLDRSDVDTDQIIPSDWLKRVERTGFGEGLFS
                     EWRESSDFVLNQAQFQGDDRRVLVAGPNFGTGSSREHAVWALQDYGFEAVIAPRFGDI
                     FRNNALKAGLVPVVLPEPVVRRILDAIEDDPTLEVVVDVVDRRVAVPALDVDEPFDLD
                     DHTHHRLVNGLDDIGLTLQHADEIAGYEARRGDWLPSVTPTTPR"
     gene            complement(1864..2532)
                     /locus_tag="VIJ44_04690"
     CDS             complement(1864..2532)
                     /locus_tag="VIJ44_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014445983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEY5165229.1"
                     /translation="MNVLAVGAHPDDVELGCGATLVRHVARGDDVTILVMTAGQRGLV
                     EGMSRRAEQQGAASNLGVRLRWGGFEDGSVPDGPDAITVIDEAIAASGAETLYTHAPH
                     DTHQDHRATATASLAAARRLPTVLQFETPSTQGFEPTIYVDVADTLEQKMVALRSHLS
                     QVLREGPVDLEAIEAQARFRGFQGRIRYAEAFEAARLAWDLATPANSEALTPVRSLSV
                     VSSA"
BASE COUNT          403 a          922 c          941 g          388 t
ORIGIN      
        1 cgcctgtcgg gtcggcccgt ccgccggccc gacctgacgg tcgccacgat ggaccacaac
       61 gtccccacga ccgacatcga ccggccgatc gccgacccga tctccgccaa gcagatcgcg
      121 gtgctggccg cgaactgcgc cgagttcgga gtgcggctct acaagatggg cgacccgggg
      181 caaggcatcg tccacatcat cggccccgaa caagggctca ccctcccggg gatgacgatc
      241 gtgtgcggcg acagccacac gtcgacccac ggggcgttcg gggcgctggc gttcgggatc
      301 ggcaccagcg aagtggaaca cgtcctcgcc acccagacgc tgccccaggc ccgaccgggc
      361 accatggcgg tgaccgtcga gggtgacctc cccccgggcg tgaccgccaa ggatgtggtg
      421 ctgtcgatca tcgcccgcat cggcaccgcg ggcgggatcg gctcgatcat cgagtaccgg
      481 ggctcggcga tccgggccct ctcgatggag ggccggatga cgatctgcaa catgtcgatc
      541 gaggccggcg cccgcgccgg gctgatcgct cccgacgaca cgaccttcgc gtacctcgag
      601 ggtcgcccgt tcgtgccgag tggcgcggcg tgggagcggg ccctcgacga atggcggtcg
      661 ttggccaccg acgacgacgc caccttcggt cgagtggtgc acctcgacgc cgccgagctc
      721 cgcccgtcgg tgtcgtgggg cacgaacccg ggtcagacgg tgacgatcga cgatcgggtc
      781 cccgaccccg actcgttcgg cgagccccac cagcgcgacg cggcccggct ggcgttgcgc
      841 tacatgggac tggaagcagg caccgcgatg cgcgacatct cggtcgacac cgtgttcatc
      901 gggtcgtgca ccaactcacg catcgaggac ctgcgttccg ccgccggggt gctggaggga
      961 cgctcagtga gggacgggct ccgggcactg gtcgtgcccg gctcgcatcg ggtgaaggcc
     1021 caagccgagg cggagggcct cgaccgggtg ttcagctcgg cggggttcga gtggcgagag
     1081 cccggctgct cgatgtgcct ggccatgaac ccggacaagc tggcccccgg cgagcgttgc
     1141 gcctccacct ccaaccggaa cttcgaaggc cgccagggca agggtggccg aacccatctg
     1201 gtgtcaccgg cggtggccgc cgccaccgcg gtggcgggcc ggttcgcggc ccccgaggac
     1261 ttggagtagc ggtggacccg gtacgtgaga tcgtgggtcg agcggtgccg ctcgaccgct
     1321 ccgacgttga cactgaccag atcatcccga gtgactggtt gaagcgcgtc gagcgcaccg
     1381 gcttcgggga gggtctgttc tcggaatggc gggagagcag cgacttcgtc ctgaaccagg
     1441 cccagttcca aggcgacgac cggcgggtgc tcgtggccgg ccccaacttc gggaccggct
     1501 cgtcacgcga gcatgcggtc tgggccttac aggactacgg gttcgaggcc gtcatcgcac
     1561 cccggttcgg cgacatcttc cgcaacaacg cgttgaaggc cgggctggtc ccggtcgtgc
     1621 tccccgagcc ggtcgtgcgt cgcatcttgg acgcgatcga agacgacccg accctcgagg
     1681 tcgtcgtcga tgtcgtcgac cgtcgggtcg cggtgccggc cctggacgtg gacgagccgt
     1741 tcgacctcga tgaccacacc catcaccggc tcgtgaacgg cctcgacgac atcggcctga
     1801 ccctgcagca cgccgacgag atcgccggct acgaggcccg gcggggcgac tggctcccga
     1861 gcgttacgcc gacgacacca cgctgagcga ccgcaccggg gtgagcgcct cggagttggc
     1921 cggggtggcg aggtcccacg ccaggcgggc cgcttcgaac gcctccgcgt agcggatccg
     1981 gccctgaaag ccccggaagc gggcttgggc ctcgatggct tcgagatcga cgggaccttc
     2041 gcggaggacc tgagagaggt gggagcgcaa cgcgaccatc ttctgctcga gggtgtcggc
     2101 cacatccaca tagatggtgg gctcgaaacc ctgcgtcgag ggcgtctcga actgcagcac
     2161 cgtcgggagc cggcgggccg cggcgaggct cgcggtggcg gtcgcccggt gatcctgatg
     2221 ggtgtcgtgg ggggcgtggg tgtagagcgt ctcggcgccg ctcgccgcga tggcctcgtc
     2281 gatcacggtg atggcgtcgg gcccgtcggg caccgagccg tcctcgaatc cgccccagcg
     2341 cagccggaca ccgaggttcg aggctgcgcc ttgctgctcg gctcgacggc tcatgccttc
     2401 cacgagacct cgctgcccgg cggtcatcac caagatggtg acgtcgtcgc cgcgggcgac
     2461 gtggcggacc aacgtggccc cgcagcccaa ttccacatcg tcggggtgag ccccgaccgc
     2521 cagaacgttc accgccctgc ccctttccac ccgtttgatg ggacctgaaa aggatcggct
     2581 ggcccggcgc ggacctgaag cccaattcac cacggagagt gggctgaacg gccggccggc
     2641 ccgcaccgga gtcc
//
LOCUS       DATCCH010000238         2098 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_565033, whole genome shotgun sequence.
ACCESSION   DATCCH010000238 DATCCH010000000
VERSION     DATCCH010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565033"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..947
                     /locus_tag="VIJ44_04695"
     CDS             150..947
                     /locus_tag="VIJ44_04695"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY5165230.1"
                     /translation="MTVAGRRVELARIGSGEPLLYLHGLADVHSALPPDPPTRLLHGL
                     AGSAAVVAPALPGYAGSDSLGPRADVEDHAFHLVDLLDEIGIGPVDVVGASFGGWLAA
                     EFALRHPARVRRLVLAAPLGLHVRDAPGVLFFGAAAPRGVGGFGEVRGVLFADPDGPV
                     ALDTLPDEIPRERALRWFGGLGAAAALGWRAPQLCDPKLADRLDRIEIPTLVVWGAGD
                     RVAPVGQAIAWERGLPDARLEVVDGAGHALCLEEPDRLADLVRDFLH"
     gene            complement(995..1831)
                     /locus_tag="VIJ44_04700"
     CDS             complement(995..1831)
                     /locus_tag="VIJ44_04700"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013423645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEY5165231.1"
                     /translation="MDHALTDVVLVDVNERVATITLNRPEVRNAINGELGAALARAVG
                     DFEARDDVDVMILTGADPAFCAGADLNEISAGRDPAGLLTDPNDPYRRDEVGKFVFRG
                     PFPPRTKLLIGAINGPAITGGFELALNCDLLVASERARFADTHARVGVMPGWGLTVLL
                     VEAVGLRRARELSTTGNFCSAEQALTWGLVNHVVAHDELLPFSRQLALAAVHNDTAGV
                     RRMLATYDQIAASMYDAGWAIEDAVNREWWTQTFDPSELVRRRGGVIARGRRQVDERD
                     RT"
     gene            complement(1816..>2098)
                     /locus_tag="VIJ44_04705"
     CDS             complement(1816..>2098)
                     /locus_tag="VIJ44_04705"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5165232.1"
                     /translation="EKRSDEMPHITKRPSECLADGRLVVSCEAERHLGHALAGLGDQN
                     VVFASDYPHWDAEFPNSVRAITSHADLDENQKSAVLGTNAARVYGWTTR"
BASE COUNT          263 a          691 c          808 g          336 t
ORIGIN      
        1 catcgggtcg ggacggtact ggggttgttg tcgttcggct ctctgccggc cggcctcgcg
       61 cgccgcagcc tcgagcgcta cgcgcaggag gtggtgccgt gggtgcgcga acacgccgcg
      121 gcgacgctgg cggccggcga gtgagctccg tgaccgtcgc cggccggcgg gtggagttgg
      181 cgcgcatcgg ctcaggcgag ccgttgctct acctgcacgg tctcgccgac gtgcacagcg
      241 cgctgccgcc ggacccgccg acccggctcc tgcacggctt ggccggctca gccgccgtgg
      301 tggcgccggc actgccgggg tacgcgggtt ccgacagcct cggccctcgg gccgacgtgg
      361 aggaccacgc gtttcacctc gtcgacctgc tcgacgagat cgggatcggg cccgtcgacg
      421 tggtgggggc ttcgtttggc ggctggttgg cggccgagtt cgcgttgcgc caccccgccc
      481 gggtgcgccg cctggttctc gcagccccgc tcgggctcca cgtgagagac gcccccgggg
      541 tgctgttctt cggcgcggcg gcgccacgcg gggtcggcgg cttcggagag gtgcgcgggg
      601 tgctgttcgc cgacccggac ggcccggtcg cgctcgacac cctgcccgac gagatccccc
      661 gcgagcgtgc gctgcgatgg ttcggcgggt tgggcgccgc cgccgcgctg ggctggcggg
      721 cgcctcagct gtgcgacccg aagctcgccg accggctgga ccgcatcgag ataccgacgc
      781 tggtggtgtg gggcgctggc gaccgggtcg ccccagtcgg ccaggcaatc gcgtgggagc
      841 gcgggctgcc cgacgcgcgg ctcgaggtcg tcgacggggc cgggcatgcc ctctgcctcg
      901 aggaaccgga tcggctcgcc gatctggtgc gcgattttct gcattgagcc cggccgagga
      961 atcggtccca gatccgttcg gcccgtcggg tggttcaggt tcggtcgcgc tcgtcgacct
     1021 ggcgccgacc ccgggcgatg accccgccgc gacgtcggac gagctcggac gggtcgaagg
     1081 tctgggtcca ccactcgcgg ttcacggcgt cctcgatggc ccaacccgcg tcgtacatcg
     1141 acgccgcgat ctggtcatag gtcgcgagca tgcgacgaac gccggcggtg tcgttgtgga
     1201 ccgcggcgag cgcgagctgg cggctgaacg ggaggagctc gtcgtgggcc acgacgtggt
     1261 tcacgagacc ccacgtcagc gcctgttcgg cagaacagaa gttgccggtg gtgctgagct
     1321 cccgggctcg gcggagcccg acggcttcga cgagcaggac cgtgaggccc cacccgggca
     1381 tgacgccgac ccgagcgtgg gtgtcagcga agcgggcccg ctcggaggcg acgagaaggt
     1441 cgcagttgag ggcgagctcg aaaccgccgg tgatcgcggg gccgttgatc gccccgatga
     1501 ggagcttggt gcggggtggg aacgggcccc gaaagacgaa cttgccgact tcgtctcgtc
     1561 ggtacgggtc gttcggatcg gtcagcaatc ccgccgggtc gcgtccggcg ctgatctcgt
     1621 tgaggtcggc gccggcgcag aacgcagggt cagcgccggt gaggatcatg acgtcgacgt
     1681 cgtcgcgtgc ctcaaagtca ccgacggcac gcgccaacgc ggcgccgagc tcgccgttga
     1741 tcgcgttgcg aacctcgggg cggttgagcg tgatggtggc gacccgttcg ttcacgtcga
     1801 cgaggacgac gtcggtcaac gcgtggtcca cccgtagacg cgagcggcgt tggtgccgag
     1861 gaccgcggac ttctggttct cgtcgagatc ggcgtggctg gtgatggccc gcaccgagtt
     1921 cgggaactcg gcgtcccagt gggggtagtc ggaagcgaag acgacgttct ggtctccgag
     1981 gccggcgagc gcgtgcccga ggtgacgttc ggcctcgcag gagacgacga gtcgaccgtc
     2041 ggcaagacac tcgctcggcc gtttggtgat gtgtggcatc tcgtcggatc gcttctcg
//
LOCUS       DATCCH010000239         3716 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_565116, whole genome shotgun sequence.
ACCESSION   DATCCH010000239 DATCCH010000000
VERSION     DATCCH010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565116"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="VIJ44_04710"
     CDS             <1..643
                     /locus_tag="VIJ44_04710"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEY5165233.1"
                     /translation="PALPLGIVLACMVGLRRLGLDRANLAAWREFLVVSGAVLVVGIW
                     QYSARVAGWREAIGLVLAALDEELVYRLAVLILVGALSAALLGRNWRNPEDWGLAPGL
                     TALLMSGLVFALLPGHVAQMNGTLRALPFVSLGIVLGYAVLRTGALFPAVVVHASLNL
                     VTIAALSGHVSNLWRASFAATALVALIASTIVAGVRLGMLQRISVRSAWWHRA"
     gene            787..2091
                     /locus_tag="VIJ44_04715"
     CDS             787..2091
                     /locus_tag="VIJ44_04715"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5165234.1"
                     /translation="MHTKARRRRRLWSGSILVTGVALAMSTFAVSPALGQSGSPTEVV
                     RIGALLSITGGGSSLGNTSRAALEVARDQWNQRLARSHQNRRVELDVIDTGQDPARAT
                     AGFTQLANQGVRVIVGPQSSSEVAAIQPLANARGVLIVSQGSTASSLAQPDDNVFRFV
                     PNDHVEGRATTDLVEQQGARAVVPMWRNDRGNQGLSDSVRAAATTDGLQVTRGVRYEP
                     ATTDFGPAVAELARQVAGAVQQFGAAHVAVYLAGFEEVANVLPAAAQIPGLAAVRWYG
                     GDGSAQAPQLIDTAGSAAFATTTRGYASPLVALPADRAKRDAGLIAQIARRAHATPDA
                     FALAAYDAFDVGVQTLLKTSPTVDGPTLRAAFRTTATAFAGVTGTIRLDPAGDRASAP
                     YAYWSICQPRGRRAQWVRTGTWTPNSATATGRGTVSGGSCPR"
     gene            2191..3108
                     /locus_tag="VIJ44_04720"
     CDS             2191..3108
                     /locus_tag="VIJ44_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEY5165235.1"
                     /translation="MTTTIERFAADRDAGAICAAIERDGVAIVEDLLSPDVVTRVNDE
                     VEAAVTAADPDEEMFNPVMQAFHGPCTKQVTGVPGISPTFATDVMCDPLLLSICDQIL
                     LPSCARYQLNLGHLLQRGPGAEEQWLHRDEAVWSDVPQPHPELQLASVIAFVDFTREN
                     GATRIIPGSHRFDDRVLSPAEQMQRSRPDPDELAYAEMPAGSAVVYVGGTIHGGGANQ
                     TQQPRRGAHLSYCLGWLRTEENNYLAVPPEIAATLPRAAQELLGYAVHDSIGRGGGYL
                     GMLRMQDPVELLARGELPRRPVSVDHLGS"
     gene            3161..3622
                     /locus_tag="VIJ44_04725"
     CDS             3161..3622
                     /locus_tag="VIJ44_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013806864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY5165236.1"
                     /translation="MTNNRDVETVERVIPAPADKIFELLADPRHHPEIDGSGTVRKSK
                     EAPERLSLGSQFGMAMKLGVPYSMVSEVIEFEDGRRIAWQTRPPSPLGARLAGGRIWR
                     YELEPVADGTRVRETWDISQEKVKAFVRPARKKTIAAMEQTLARIEELVAK"
BASE COUNT          569 a         1356 c         1243 g          548 t
ORIGIN      
        1 gccggcgctt ccgctcggga tcgtcctggc gtgcatggtg ggcttgcgtc gcctcggcct
       61 cgaccgcgcc aacctggcgg cctggcggga gttcctggtc gtcagtgggg cggtcctcgt
      121 cgtcgggata tggcagtaca gcgcgcgggt cgccggctgg cgcgaggcga ttggactggt
      181 gctcgcggcc ctcgacgagg agctggtcta ccgactcgcg gtgctcatcc tcgtgggagc
      241 gctgagcgcg gcgctcttgg gacggaactg gcgcaacccc gaagactggg ggctcgcccc
      301 gggcctcact gccctgctga tgtcgggtct ggtcttcgcc ctcttgcccg gtcacgtggc
      361 ccagatgaac ggcacgctcc gggcgttgcc gttcgtgtcc ctggggatcg tgttgggata
      421 cgccgtcttg cgcacggggg cactgtttcc cgccgtggtc gtgcatgcgt ccctgaactt
      481 ggtcaccatc gccgcactca gcggacacgt ctccaacctg tggagggcat cgttcgcggc
      541 gacggctctg gtggccttga tcgcgagcac gatcgtggcc ggggtgcgcc tcgggatgct
      601 gcagcgcatc tcggttcggt cggcgtggtg gcatcgcgcc tgaatcggcg gcacagagcc
      661 aacaccggcc gcggccgttc cagaagttgg cgaccccgtc gacggacctc gcgaaccgcg
      721 atccgcgggc gtggtaaaca gcgccgcgct gaccgtcgtt gccgcgaacc acgaggggaa
      781 ggtgcgatgc acacgaaagc ccgtcgccgt cgtcgactct ggagcgggtc gattctcgtc
      841 accggtgttg cgctggcgat gtcgacgttc gcggtcagcc ccgcgctcgg tcagtcgggc
      901 tcgcccaccg aggtggtgcg catcggcgcc ttgctgtcca tcaccggggg cggctcgagc
      961 ctcggcaaca cgagccgcgc ggcgctcgag gtggcccgcg atcaatggaa ccagcgtttg
     1021 gcgcgcagcc accagaaccg ccgcgtcgaa ctggacgtca tcgacaccgg ccaggatccc
     1081 gcgcgggcaa cagccgggtt cacgcagctg gcaaaccagg gggtccgggt catcgtcgga
     1141 ccacagtcga gctccgaggt cgccgccatc cagccgctgg cgaacgcccg cggggtgctc
     1201 atcgtctcgc aaggaagcac cgcgtcgtcg ctggcgcagc cggacgacaa cgtgttccgc
     1261 ttcgtcccca acgaccatgt cgaagggcgc gccaccaccg acctcgtcga acagcaaggt
     1321 gcccgagcgg tcgtgccgat gtggcgtaac gaccgcggca accagggtct gtcggactcg
     1381 gtgcgcgccg ccgcgaccac cgatggtctc caggtgaccc gcggggttcg gtacgagccg
     1441 gccacgacgg acttcggccc cgccgtcgcc gaactcgccc gccaggtcgc cggtgcggta
     1501 cagcagttcg gcgctgccca cgtcgcggtc tacctcgccg gcttcgagga ggtcgcgaac
     1561 gtcctgcccg ccgcggcgca gatccccggg ctcgcggcgg tgcgctggta cggcggggac
     1621 gggtccgcac aagcccccca gctcatcgac accgccgggt cggccgcctt cgcgaccacg
     1681 acccgcggct acgccagtcc tctcgtggcg ttgcccgccg accgggccaa gcgtgacgcc
     1741 gggctcatcg cccagatcgc ccgccgagcg cacgcgaccc cggacgcgtt cgcgctcgcc
     1801 gcgtacgacg ccttcgacgt cggggtgcag accctgctca agaccagccc gactgtcgac
     1861 ggcccgaccc tgcgggccgc gttcagaacc acggccaccg cgttcgccgg tgtgaccggc
     1921 accatccggc tcgacccggc cggggaccgc gcctcggctc cgtacgccta ctggtcgatc
     1981 tgtcagcccc gaggccggag ggcccaatgg gtccgcaccg gcacctggac tccgaacagc
     2041 gccacggcca ccgggcgggg gacagtcagc ggtgggagct gtccgcgctg agaccgccgg
     2101 ggttgccccg aggccaccac ggcacgactc gggacggtgg tgtcacactc ttcgcaccga
     2161 cgcagctcgg tgccagcaga ggagtcgggg atgacgacga ccatcgagcg attcgcggct
     2221 gaccgcgacg cgggggccat ctgcgccgcg atcgaacgcg acggtgtcgc gatcgtcgag
     2281 gacctcctgt ctcccgacgt cgtgacccgc gtgaacgacg aggtcgaagc ggccgtgacc
     2341 gccgccgatc cggacgagga gatgttcaat ccggtgatgc aggcctttca cggcccgtgc
     2401 acgaagcagg tcaccggggt gccagggatc tcgccgacgt tcgcgaccga cgtgatgtgc
     2461 gatccgcttc tcctctcaat ctgtgaccag atcctgttgc cgtcgtgcgc ccgctaccag
     2521 ctcaacctcg gccatctgct gcaacgcggc cccggagccg aggagcaatg gctgcaccgt
     2581 gacgaagccg tctggagcga cgtcccgcaa ccacatcccg agcttcagct cgcgtccgtc
     2641 atcgcgttcg tcgacttcac ccgcgagaac ggggccaccc ggatcatccc cggaagtcac
     2701 cgcttcgacg accgggtcct gagccccgcc gagcagatgc aacgatcccg gcccgacccc
     2761 gacgagctcg cctacgccga aatgccggcc ggttccgccg tcgtctacgt cggcgggacc
     2821 atccacggcg gtggcgccaa ccagacgcag caaccccggc gcggcgccca cctcagctac
     2881 tgcctcggct ggctgcgaac cgaggagaac aactatctcg cggtgccacc cgaaatcgcg
     2941 gcgaccctcc cccgcgccgc ccaggagctc ctgggctacg cggtgcacga cagcatcggc
     3001 cggggcggtg gctacctcgg catgctgcgc atgcaggacc ccgtcgagct cctcgcccgg
     3061 ggcgagttgc cccgaagacc cgtcagcgtc gaccatctcg gctcgtaact cgaccggcga
     3121 gcccggaacg gcccgatagt ccctgggcta ccgttcggcc atgacgaaca accgcgacgt
     3181 tgagaccgtc gagcgcgtca tccccgcgcc cgccgacaag atcttcgagc tgctcgccga
     3241 cccgcgacat caccccgaga tcgacgggtc ggggacggtc cgcaaatcca aggaagcccc
     3301 cgagcggctc tcgctcggct cacagttcgg gatggcgatg aagctcggtg tcccgtactc
     3361 gatggtcagc gaggtcatcg agttcgaaga tggccgccgc atcgcctggc agacccgacc
     3421 gccgagcccg ctcggtgctc gactcgccgg tggacggatc tggcgctacg agctcgaacc
     3481 ggtcgccgac ggcacccggg tgcgggagac ctgggacatc tctcaggaga aggtcaaggc
     3541 tttcgtccga ccagcgcgca agaagaccat tgccgcgatg gaacagaccc tggcccgcat
     3601 cgaagagctc gtcgcgaagt aggcccggca cccaagccac gcgcgacacc ccatcttgtc
     3661 gccggcagtg tccacaccga tgacccccga ctcgtcgaaa cccgccgacg tggcct
//
LOCUS       DATCCH010000240         2549 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_192475, whole genome shotgun sequence.
ACCESSION   DATCCH010000240 DATCCH010000000
VERSION     DATCCH010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192475"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1531
                     /gene="polA"
                     /locus_tag="VIJ44_04730"
     CDS             <1..1531
                     /gene="polA"
                     /locus_tag="VIJ44_04730"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEY5165237.1"
                     /translation="SLLAAGGPPLIAHRAKDLSHGLGDDIRTLHQDTAVMAYLLDPAE
                     GKYRLEDLALRYLSLEISSPDREEGRLDLDGQAGIEETGRRAVAILRLADVLSEALTA
                     RELTDLYDRIERPLVRVLAKMEEAGIRIDQPFLDDLRSELAKECDQLMRQIWAHAGEE
                     FNVNSTPQLRTILFDKLGLIPVKKTKTGASTDADSLQKMVDEHPIVEDLLRYREVEKL
                     RSTYADALPPLIGPDGRVHATFKQTDTTTGRISSEAPNLQNVPVRTADGREFRRVFIA
                     DDGWGLLTADYSQIELRVLAHLAEDPGLTEAFARGADIHTTTAATVFHVAEGDVDAAQ
                     RRFAKVVNYGLAYGMEAYGLGSRLGIPTEEAREILDAYFDGFPNVAEFMARTVREAKE
                     RGYTTTIFGRRRQITELASDNFRIRQMGERMAQNAPVQGSAADIFKLAMIDVDRALET
                     AGSKTRMLLTVHDELVFEVPEEERTDVEALIRDVMETVTELTVPLVVDVGFGPNWAQA
                     K"
     gene            1579..2334
                     /locus_tag="VIJ44_04735"
     CDS             1579..2334
                     /locus_tag="VIJ44_04735"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY5165238.1"
                     /translation="MATPPVPAGPWFNEVASFLGRAYWAPGTTRVSSFTTGTEQEVDF
                     LVETLGLRAGQTVLDVGCGPGRHALALARRGLRVTGVDASQEFVALATAAAAGDGLSA
                     QFTVLDVRDLVSDGAYDAVICLCQGGFGLLGGRDDVDVFARIAGAVRPGGGIAVSAFS
                     VLFAARFLEAGEMLDAATGVLHERATLRNEDGEERPMDLWTTCFTTRELELLAALSDV
                     EVRGVYGVTPGRYRAAAPDLDQPELLLVGHRRA"
BASE COUNT          402 a          874 c          895 g          378 t
ORIGIN      
        1 atccctcctc gcggcgggcg gtccgccgtt gatcgcgcac cgggcgaagg acctcagcca
       61 cggtctcggt gacgacatcc gcaccctcca tcaggacacc gcggtgatgg cgtatctcct
      121 cgatcctgcc gaggggaagt atcgactcga ggacttggcg cttcggtacc tctcgctcga
      181 gatcagctcc ccggaccggg aggaaggccg cctcgatctc gatggccaag ccgggatcga
      241 agagaccggt cggcgcgcgg tggcgatcct gcgactcgcc gacgtgctgt cggaggcgtt
      301 gacggcccga gagctcaccg atctctacga ccggatcgaa cgaccgttgg tccgggtgct
      361 ggcgaagatg gaagaggcgg ggatccgcat cgaccagccg ttcctcgacg acctgcggtc
      421 cgagctggcc aaagagtgcg accagctgat gcgccagatc tgggcccatg ccggcgagga
      481 gttcaacgtg aactcgacgc cgcagctgcg cacgatcctc ttcgacaaac tcgggctcat
      541 tccggtgaag aagaccaaga ccggcgcgtc caccgacgcc gactcgttgc agaagatggt
      601 ggacgagcac ccgatcgtcg aggacctgtt gcgctaccgg gaggtcgaga agctacgcag
      661 cacctacgcc gacgcgctcc cgccgctgat cggcccggat gggcgggtcc acgcgacgtt
      721 caagcagacc gacaccacga ccggccggat ctcgagcgaa gccccgaacc tccagaacgt
      781 cccggtccgc accgccgacg ggcgagagtt ccgtcgggtc ttcatcgccg acgacggctg
      841 gggactgctc accgcggact actcccagat cgagctgcgg gtcctcgcgc acctcgccga
      901 ggatcccggt ctgaccgagg cgttcgcccg aggcgctgac atccacacca cgaccgcggc
      961 cacggtgttc cacgtcgcgg agggcgacgt cgacgcggcg caacgacggt tcgcgaaagt
     1021 ggtcaactac gggctggcat acggcatgga ggcctacggg ctcggctccc ggctcggcat
     1081 cccgaccgag gaggcccgcg agatcctcga cgcgtacttc gacgggttcc cgaacgtcgc
     1141 cgagttcatg gcccggacgg tccgcgaggc caaggaacgg ggatatacga cgacgatctt
     1201 cggtcgccga cgccagatca ccgagctcgc ctccgacaac ttccggatcc gccagatggg
     1261 ggagcggatg gcgcagaacg cgccggtgca gggctccgcg gcggacatct tcaagctcgc
     1321 gatgatcgac gtcgaccggg ccctcgagac tgcgggttcg aagacccgga tgctgctcac
     1381 ggtgcacgac gagctcgtct tcgaggtccc ggaggaggag cggaccgacg tcgaggcgct
     1441 gatccgggac gtcatggaga ccgtcaccga gctgaccgtc ccgctggtgg tcgacgtcgg
     1501 gttcgggccg aactgggccc aggccaaatg agtgggggtc cgcccccggc atcctctctt
     1561 taggggccgt ggcgacccgt ggcgaccccg ccggtcccgg ccgggccctg gttcaacgag
     1621 gtcgcgtcgt tcctgggtcg cgcctactgg gcgccgggca cgacccgggt gagttcgttc
     1681 accaccggca ccgaacaaga ggtggacttc ctcgtcgaga ccctcgggtt gcgggccggc
     1741 cagacggtgc tcgacgtcgg ctgcggcccc gggcgccacg cgctggcgct cgcccggcgg
     1801 ggacttcggg tcaccggggt cgacgcctcc caggagttcg tggccttggc gaccgcggcc
     1861 gcggctggcg acggtctctc agcccagttc acggtcctcg acgtccgtga cctggtcagc
     1921 gacggcgcgt acgacgccgt gatctgtctc tgccaaggcg gattcgggct cctcggcgga
     1981 cgcgacgacg tcgacgtctt cgcccgaatc gcgggcgcgg tgcgtcccgg cggcgggatc
     2041 gccgtgtcgg cgttctcggt gctgttcgca gcccggttcc tggaagccgg cgagatgctc
     2101 gacgcggcca ccggggtcct gcacgaacgg gcgacgctgc gcaatgaaga cggcgaggag
     2161 cgcccgatgg acctctggac cacctgcttc accacgcgag agctcgagct gctcgccgcg
     2221 ttgagcgacg tcgaggtccg cggcgtctac ggggtgacac cgggtcgcta ccgcgccgcg
     2281 gcgccggacc tcgaccagcc cgagttgctg ctggtggggc accgccgagc atgagcctgt
     2341 agcctggctg agcggcctcg ttgaggccgc gccattcgcg cttggacacg atcggagacg
     2401 cgggcggccc ggtattttga ggagtcaggt tgtcggagca ggacgcggcg ggaggtaccg
     2461 gcacccccgt cctcgagtac gagcccagtc cgggcgaagc cggcgccccc ggagacggtc
     2521 cggtcgtcct cgacgacctc ggaggccag
//
LOCUS       DATCCH010000241         3994 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_54466, whole genome shotgun sequence.
ACCESSION   DATCCH010000241 DATCCH010000000
VERSION     DATCCH010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54466"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3029
                     /gene="dnaE"
                     /locus_tag="VIJ44_04740"
     CDS             <1..3029
                     /gene="dnaE"
                     /locus_tag="VIJ44_04740"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEY5165239.1"
                     /translation="AGRFQDVVGPDQFFIELQDHGLTDQQRVNPGLLRIARDLHAPLL
                     ATNDSHYTNREDAEAHDALLCVQTGSTRDDPKRFKFEGEEFYLKSAAEMRALFADYEE
                     ACDNTLLIAERANVEIEFDNAVLPAFATPAGHDEASYLRELTVNGATNRYGDPMPVNV
                     AQRIDYELGVIKTMGFSAYFLVVWDLVRYARERRIRVGPGRGSAAGSCVAYCLGIVDI
                     DPIRYDLLFERFLNPGRAQMPDIDMDFDSRSRGEMIRYAAERYGADHVAQIVTFSTIK
                     ARAAVRDGARVLGYPYGMGDRIAKLMPPLIMGRDTPLQACLEPVPGHDDGYKMAVELR
                     ELYGEDPDARRVIDVARGLEGLRRQDGIHAAAVVITREPLTEYLPVQRKPEPGRDLAD
                     APIVTQYEMHGVEDLGLLKMDFLGLRNLDVLEITLDLVEASSGQRPDIDHVALDDPMT
                     FDLLRRGDTVGVFQLEGAQVRALLQRLAPTSFEDIAALVALYRPGPMAQNWHTEYADR
                     KNGRRLVDYPHPDLEEILRPTYGLMIYQEQLMRVAQQLAGYTLEEADNLRKATGKKIR
                     SLITQERSKFVDGCVAQGHARAFGEKIFDTIEPFADYSFNKSHSVGYGYLAYQTAFLK
                     ANYPVEYLAALLTSVRTNKDQTAVFLNECRQLGISVLVPDVNESVSDFSVHVAPDGPR
                     SIRFGLSAVRNVGEGVTAMIVAARTEAGPFRDFYDFCGRVDPSALNKRTIESLIKAGG
                     FDSLGHPRQGLLHVFEPLVEQTVSRRREREAGIMSLFGDGTTDGGDTPALSERVEIPE
                     SEFDRKTRLDFEKEMLGLYVSDHPLLGTERVLRRYIDCTLGELRDAREGELRTVGGIV
                     TGLARKYTKKGDLMATFVLEDLGAALEVMVFPKTMADHGHLLEDDAIVCVKGRLDRRE
                     DQPKIVAMELTRPEISLDGAPPVRLRTAMAKLSDDRVDKLTEVLRRHPGESPVFVHLE
                     RPERTTVLRLADDFRVDATNGLFAELRILLGPDCIC"
     gene            complement(3180..3380)
                     /locus_tag="VIJ44_04745"
     CDS             complement(3180..3380)
                     /locus_tag="VIJ44_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165240.1"
                     /translation="MSDPPRPRAPASLRVVGVVVLALLAWLLLGSALSVARAVIALAG
                     YVIVAVVAYLVGKWVGRHSAPS"
     gene            3413..>3994
                     /gene="npdG"
                     /locus_tag="VIJ44_04750"
     CDS             3413..>3994
                     /gene="npdG"
                     /locus_tag="VIJ44_04750"
                     /inference="COORDINATES: protein motif:HMM:NF015747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HEY5165241.1"
                     /translation="MCRRAMLGRMEIGVLGGTGPAGRGLAARLADAGFDVIAGSRDET
                     KASSVVDELRHRWGSRVAKLRPGTNATAAGARDVVVIATTWEAAIDTTEAHAPPLAGK
                     VVVAMANGLQRVGREFRAVLPPEGSVAAAMQARVPDARVVAAFHFIPADALAKLDTPI
                     ESDIIACGDDDDARRTVRGLATSIPNLRAFDGGA"
BASE COUNT          663 a         1443 c         1293 g          595 t
ORIGIN      
        1 tcgccggccg cttccaagac gtcgtggggc ccgatcagtt cttcatcgag ctccaagacc
       61 acgggctcac cgaccaacag cgagtcaacc ccggcctgtt gcgcatcgcc cgagacctcc
      121 acgcaccgct cttggccacg aacgacagtc actacaccaa ccgggaggac gcggaggcgc
      181 acgacgcgct gctctgtgtc cagaccgggt ccacgcgcga cgacccgaaa cggttcaagt
      241 tcgaaggtga agagttctac ctcaagtccg cggccgagat gcgggccttg ttcgccgact
      301 acgaggaggc gtgcgacaac acattgttga tcgccgagcg cgccaacgtc gagatcgagt
      361 tcgacaacgc ggtgctgccc gccttcgcga cccccgcggg ccatgatgag gcctcctacc
      421 tgcgcgagct caccgtgaac ggtgccacga accgatacgg cgatccgatg cctgtgaatg
      481 tggcgcagcg catcgattac gagctcgggg tcatcaagac gatggggttc tcggcgtact
      541 tcctcgtggt ctgggacctt gtccgctacg cccgggagcg acgcatccgg gtcggccccg
      601 gacgggggag cgccgcggga tcgtgcgtcg cgtactgcct cggcatcgtc gacatcgacc
      661 cgatccgcta cgacctgctg ttcgagcgct tcttgaaccc cggccgggcc cagatgcccg
      721 acatcgacat ggacttcgac tcccgctcac gtggcgagat gatccggtac gccgcggagc
      781 gctacggcgc cgaccacgtc gcccagatcg tcaccttctc gacgatcaag gcccgcgccg
      841 cggtgcggga cggggcccgg gtgctcggct acccgtacgg gatgggggac cggatcgcga
      901 agctgatgcc gccgttgatc atgggccgcg acacgccgct gcaggcctgt ctcgaaccgg
      961 tccccggtca tgacgacgga tacaagatgg cggtcgagct ccgagagctc tacggcgaag
     1021 accccgacgc ccgtcgggtg atcgacgtcg cccggggtct cgaaggcctc cgccgccagg
     1081 acgggatcca cgccgccgcg gtcgtcatca cccgcgaacc gctgaccgag tacctccccg
     1141 tccagcgcaa gccggaaccg ggccgcgacc tcgccgacgc gccgatcgtc acccagtacg
     1201 agatgcacgg tgtcgaggac ctgggcctgc tcaagatgga cttcctcggg ttgcggaacc
     1261 tcgacgtgct cgagatcacc ctcgatctcg tcgaggcttc cagcgggcaa cgccccgaca
     1321 tcgaccacgt cgcgctcgac gacccgatga ccttcgactt gctccgtcgc ggggacaccg
     1381 tcggtgtgtt ccagctcgaa ggcgcgcagg tgcgcgcctt gctgcagcgc ctggcaccca
     1441 ccagcttcga ggacatcgcc gcgctcgtcg ccctctaccg gcctggtccg atggcccaga
     1501 actggcacac cgagtacgcc gaccgaaaga acggtcgtcg cctggtcgac tacccccacc
     1561 cggatctcga ggagatcctc cggcccacct acgggctcat gatctatcag gagcagctga
     1621 tgcgcgtcgc gcagcagctc gccggctaca cgttggagga agcggacaac ctccgcaagg
     1681 ccacgggcaa gaagatccgc tccctcatca cccaggagcg ctccaagttc gtcgacggct
     1741 gcgtcgcgca gggccacgcc cgtgcgttcg gcgagaagat cttcgacacc atcgagccgt
     1801 tcgccgacta ctcgttcaac aagtcccact cggtgggcta cggctatctc gcgtatcaga
     1861 ccgccttctt gaaggcgaac tacccggtcg agtacctggc cgccttgctc accagcgttc
     1921 gcaccaacaa ggaccagacc gcggtgttcc tgaacgagtg ccgccagctc ggcatctccg
     1981 tcctggtgcc cgacgtgaac gaatcggtgt cggacttctc ggtgcacgtc gcgccggacg
     2041 gcccccggtc gatccgattc gggctctctg cggtccgcaa cgtgggggaa ggagtcacgg
     2101 cgatgatcgt cgctgcccgc accgaggccg gtccgttccg ggacttctac gacttctgtg
     2161 gccgggtcga cccctcggcg ctgaacaagc ggaccatcga atcattgatc aaggccggcg
     2221 gcttcgactc gctcggccat ccccgccaag gtctgctgca cgtcttcgag ccgctcgtcg
     2281 aacagaccgt cagccggcgg cgcgaacgcg aagcagggat catgagcctc ttcggtgacg
     2341 gcactaccga cggaggagat acccccgcgc tctccgagcg ggtcgagatc cccgagtccg
     2401 agttcgaccg caagacccgc ctcgacttcg agaaggagat gctgggcctc tacgtcagcg
     2461 accatcccct cctcggcacc gaacgggtgc tgcgccgcta catcgactgc accctcggcg
     2521 agcttcgcga cgcccgagaa ggcgagctgc gcaccgtggg cggcatcgtc accgggctcg
     2581 cccgcaagta caccaagaag ggtgacctga tggccacctt cgtgctcgaa gacctgggcg
     2641 cggcgctcga ggtgatggtc ttcccgaaga cgatggccga ccacggccac ctgctcgagg
     2701 acgacgcgat cgtctgcgtc aagggccgcc tcgatcgtcg cgaagaccag cccaagatcg
     2761 tcgcgatgga gctcacccga ccggagatct ccctcgacgg cgcaccaccg gtccggctcc
     2821 gaacggcgat ggcgaagctg tccgacgacc gcgtcgacaa gctcaccgag gtcttgcgcc
     2881 gccaccccgg cgagagcccg gtcttcgtgc atctggagcg gccggaacgc acgaccgtcc
     2941 tgcgtctcgc ggacgacttc cgggtcgacg ccaccaacgg cctcttcgct gagctgcgaa
     3001 tcctgctcgg acccgactgc atctgctgac ctgctgcgga ccgggtcccc cccacatggg
     3061 tccggtctcg ggagatcggc ccgctacacc ggtccgtgca ccaaaggcgg tggagaactg
     3121 ccagcgctcg tcggctcccg gtgaccgagc ccgacagggg caagcctgaa tgatcgcgcc
     3181 tagctcggcg cggagtggcg cccgacccac ttgccaacta ggtaggcgac gaccgcgacg
     3241 atcacgtacc ccgccagcgc gatcaccgcg cgagcgacgc tgagcgcgga gccgagcagg
     3301 agccaggcca gcagcgcgag gacgaccacg ccgacgacgc gcagtgacgc tggcgcgcgg
     3361 gggcgagggg ggtcgctcac gcgtcgcagc gtacgccggt ctcatcgggc gcgtgtgtcg
     3421 tcgtgccatg ctggggcgca tggagatcgg tgtcctgggc gggaccggcc ccgcgggccg
     3481 gggtctcgcc gctcgcctcg cggacgccgg cttcgatgtg atcgccgggt cgcgtgacga
     3541 gacaaaggcc agctcggtcg tggacgagct tcgccatcgg tgggggagtc gggtggcgaa
     3601 gctgcggccc gggacgaacg cgaccgccgc gggtgcccgc gacgtcgtcg tgatcgccac
     3661 cacctgggag gccgcgatcg acaccaccga ggcgcacgcc ccgccgctcg ccggcaaggt
     3721 ggtcgtcgcg atggccaacg ggctccagcg cgtcggccgc gagttccgcg ccgtcctccc
     3781 ccccgaaggc tccgtcgccg ccgccatgca ggcccgcgtc cccgacgctc gggtcgttgc
     3841 cgcgttccac ttcatccccg ccgacgcgct ggccaagctc gatacgccga tcgagagtga
     3901 catcatcgcc tgcggcgacg acgacgacgc ccgccgcacg gtgcgggggc tcgccacctc
     3961 gatcccgaac ctgcgggcct tcgacggcgg ggcg
//
LOCUS       DATCCH010000242         4257 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_565844, whole genome shotgun sequence.
ACCESSION   DATCCH010000242 DATCCH010000000
VERSION     DATCCH010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4257
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565844"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="VIJ44_04755"
     CDS             complement(<1..736)
                     /locus_tag="VIJ44_04755"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEY5165242.1"
                     /translation="MASDVSLRGVYVPLITPFAPDGTVALDALDALARTYLDAGVAGL
                     VPLGTTGETPLLDHDEQAAVVDVCARVCRERGAPMIVGTGTNNTRSTIAATQALAAIP
                     ETTAALCVVPYYLRPGQAGIVAHFEAIAAASPVPVVVYNIPGRTGRLLEPDGLLRLAN
                     TPNIVGVKQAVGAVDDGSLRLLAEAPAGFAVLGGDDPFMFPLVLLGAAGAISAAAHVC
                     TRRFVEMTECGLAGKVDEGRAHHDALL"
     gene            762..1592
                     /locus_tag="VIJ44_04760"
     CDS             762..1592
                     /locus_tag="VIJ44_04760"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY5165243.1"
                     /translation="MSDLWRIVLSTVVGLGTGVLSGMFGIGGAVASTPAIRVLGASPL
                     AAVGSTLPSIIPSAVTGAFRYRREELIHWRVVVWTSGAGVAAAVGGALLTTVVPGNGH
                     PLMILTAAMVGFSAYRLSRPVEGPEPESVVAMEGITDAAPGLAHPSSGRRRDEAWRLV
                     VIGVGAGGLSGLLGIGGGVLMVPLFTSWVRLPIKEALATSLGCVGVLAVPGMITHAAL
                     GHIDWLFALPLCVGVIPGARLGAHLTIRSSDRTLRVLVGVILGLIALVYAAGEILALT
                     "
     gene            complement(1546..2283)
                     /locus_tag="VIJ44_04765"
     CDS             complement(1546..2283)
                     /locus_tag="VIJ44_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEY5165244.1"
                     /translation="MPSPLAAISAEVVSCRACPRLVAWRETVARTKRASFRDETYWGR
                     PVPGFGDPEASILLVGLAPAAHGGNRTGRVFTGDRSGDWLFGALYRAGLANQPTSVRA
                     DDGLTLHDAYVTAAVRCAPPDNAPTPEERDRCLPYLIRELALLTRIRVIVVLGGFAYA
                     ALWRTLGAAAIELPRPRPRFAHGLEVATPRATIVGCYHPSQQNTFTGRLTEAMLDDVL
                     ARAQALTAAPAGFRSGPGSRPPRTRGR"
     gene            2316..3239
                     /locus_tag="VIJ44_04770"
     CDS             2316..3239
                     /locus_tag="VIJ44_04770"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEY5165245.1"
                     /translation="MRLLLIVNPTASSMTPRRRLQVQHALGESHRLEVAETIRRGHAT
                     RLARNAAREGFDAVVVAAGDGTLNEAANGLAGTDTALAPLPGGSTNVFARTIGVANSM
                     GSATEQLVESLERRSLHRVGLGCGNGRRFLFHLGAGFDAEVIEQVERHAWLKRWLAHP
                     TFAVAAITTFYRGFDREHPPYRVEVGGEPAGDGFFAVTSNTSPYAFFGVRPLTVTHAA
                     GLDRKLALTMFRSLNTRVLLPAALSAMSRGRRLERHRQIVQLADLDALTFVAHGSPFP
                     WQVDGDYLGDVERLEVHYDPDCLTLVVPPEG"
     gene            complement(3211..3963)
                     /locus_tag="VIJ44_04775"
     CDS             complement(3211..3963)
                     /locus_tag="VIJ44_04775"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEY5165246.1"
                     /translation="MPGASPNTRVWAHRGARRQAPENTVAAFHRARDLGADGVELDVR
                     RCSDGGLVVHHDAKARGVGVLAQAPFTAVQASRPEIPTLDEALDACVGMLVNVEVKNL
                     PGDPDFDADDRIVGLLAETLARRGHTDDVLVSSFNLNTVDRFRQVPDAAPTALLTLRG
                     FEPLAAVTLAADRGHAALHPNVWGLTGPVAATVTTRAHELGLALNVWTVNRAATVRRL
                     ARAGVDAVVTDVPDVALLALGRDVSPRAEPPG"
BASE COUNT          570 a         1450 c         1633 g          604 t
ORIGIN      
        1 ccaggagtgc atcgtggtgg gcccgaccct cgtcgacctt cccggccaat ccgcactccg
       61 tcatctccac gaagcgtcgg gtgcacacat gcgccgcggc cgagatcgct cccgccgcgc
      121 cgaggaggac cagcgggaac atgaacgggt cgtcaccacc gagcaccgcg aaccccgcgg
      181 gggcctcggc aagcaaccgc agcgagccgt cgtcgacggc gccgaccgcc tgtttgacgc
      241 cgacgatgtt cggggtgttg gccagccgga ggagcccgtc gggctcgagg agccggccgg
      301 tgcggccggg gatgttgtag acgacgaccg gtaccgggct cgccgcggcg atcgcttcga
      361 aatgggcgac gatgccggcc tggccgggtc gcaggtaata gggaacgacg cacaacgcgg
      421 cggtggtctc ggggatcgcg gcgagcgcct gggtggcggc gatcgtcgag cgggtgttgt
      481 tggtgccggt cccgacgatc atcggcgccc cccgctcgcg acagacccgg gcgcacacgt
      541 cgacgacggc ggcctgctcg tcgtggtcga ggagcggggt ctcgccggtg gtgccgaggg
      601 gcacgagacc ggcgacgccg gcgtcgaggt aggtgcgcgc gagcgcgtcg agcgcgtcga
      661 gcgcgaccgt gccgtcgggc gcgaacgggg tgatgagcgg gacgtagacg ccgcgtaacg
      721 acacgtcgct ggccacgagg cgaagggtag cctccgcggg catgtccgat ctctggcgaa
      781 ttgtgttgag caccgtggtg ggcctcggca ccggcgtcct gtcgggcatg ttcggcatcg
      841 gcggggcggt cgcgtcgaca ccggcgatcc gggtgctggg cgcgtcaccg ctcgccgcgg
      901 tgggctcgac gctcccgtcg atcatcccgt cggccgtgac cggcgcgttc cgctaccggc
      961 gagaggagct catccactgg cgggtggtgg tgtggacgtc gggcgcgggc gtggcggccg
     1021 cggtgggtgg cgcgctgctg accaccgtgg tgcccggcaa cgggcatccg ctcatgatcc
     1081 tcaccgcggc gatggtcggg ttcagcgcct accggctcag ccgcccggtc gagggccctg
     1141 aaccggaatc ggtggtcgcg atggagggga tcaccgacgc cgcgccgggt ctggcgcacc
     1201 cgagctcggg gcgacgacgg gacgaggcgt ggcggctggt ggtgatcggg gtcggggccg
     1261 gtgggctcag tggcctcctc gggatcggcg gcggggtgct catggtgccg ttgttcacct
     1321 cgtgggtccg tctgccgatc aaggaggcgc tggcgacgtc gctgggctgc gtcggcgtgc
     1381 tggcggtccc ggggatgatc acccacgccg cgctcggcca catcgactgg ctcttcgcgc
     1441 ttccgctctg cgtcggcgtg atccccggcg cgcgtctcgg ggcccacctc accatccgat
     1501 cctcggatcg gacgctgcgc gtcctggtgg gagtcatcct cggcctcatc gccctcgtgt
     1561 acgcggcggg cgagatcctg gccctgacct gaacccggcc ggcgccgcgg tcaacgcctg
     1621 agcgcgggcg aggacgtcgt cgagcatcgc ctcggtgagc cggccggtga acgtgttctg
     1681 ctggctcggg tgatagcagc cgacgatcgt cgcccggggg gtcgcgacct cgagcccgtg
     1741 agcgaaccgc gggcggggtc ggggcagctc gatcgcggcg gcgccgagcg tgcgccacag
     1801 cgcggcgtag gcgaagccgc cgagcaccac gatgacgcgg atccgggtga ggagggcgag
     1861 ctcccggatg agatacggca ggcagcggtc ccgttcctcg ggggtgggcg cgttgtcggg
     1921 tggcgcgcag cggaccgcgg cggtcacgta cgcgtcgtgg agggtcaagc cgtcgtcggc
     1981 tcgcaccgac gtgggctggt tggcgagccc ggcgcggtac agggccccga agagccagtc
     2041 gccagaccgg tcgccggtga agacccgccc ggtgcggttc ccgccgtgcg cggccggcgc
     2101 cagcccgacc agcaggatcg acgcctcggg gtccccgaac ccggggacgg gacggcccca
     2161 gtacgtctcg tcgcgaaacg acgcccgttt cgtccgggcc acggtctcgc gccaggcgac
     2221 gaggcgcggg caggcccggc acgagacgac ctcggcgctg atcgcggcga gcggcgaggg
     2281 caccgacgga ccgtagcgcc gtaacctgct cggccgtgcg gctcctgctg atcgtgaatc
     2341 cgaccgcgtc gtcgatgacg ccccggcggc ggctgcaggt ccaacacgcc ctcggtgagt
     2401 cacaccgtct cgaggtggcc gagacgatcc gccgcggtca cgccacccga ctggctcgca
     2461 acgccgcccg ggagggcttc gacgccgtcg tcgtcgccgc cggggacggg acgctgaacg
     2521 aggcggccaa cgggctggca ggcaccgaca ccgcgttggc gccgctgccg ggggggtcga
     2581 ccaacgtgtt cgcccgcacc atcggggtgg cgaactcgat gggttcggcc accgagcaac
     2641 tcgtcgagag cttggagcgc cgctcactcc accgggtcgg actcggctgt gggaacggcc
     2701 gccggttctt gttccacctc ggtgccgggt tcgacgccga ggtcatcgag caggtcgaac
     2761 ggcacgcgtg gctgaaacgc tggctggcgc acccgacctt cgcggtggcg gcgatcacca
     2821 ccttctaccg aggattcgac cgcgagcatc cgccgtaccg ggtcgaggtg ggtggcgagc
     2881 cggccggcga cgggttcttc gcggtcacgt cgaacacgtc cccgtacgcg ttctttggtg
     2941 tgcgtcccct gaccgtgacc cacgccgcgg gtctcgaccg caagctggcc ttgacgatgt
     3001 ttcggagcct caacacccgg gtgctgctcc cggcagcgtt gtcggcgatg agccgaggcc
     3061 gtcggctcga acgtcaccgc cagatcgtcc agctcgccga cctcgacgcg ctcacgttcg
     3121 tcgcgcacgg gtccccgttc ccgtggcagg tcgacggcga ctatctgggc gacgtggagc
     3181 gcctcgaggt ccactacgac cccgactgcc tcaccctggt ggttccgccc gagggctgac
     3241 gtcgcggccc aaggccaaga gcgcgacgtc agggacgtcg gtgacgaccg cgtcgacacc
     3301 ggcccgggcg agacgccgca ccgtggcggc ccggttcacg gtccagacgt tgagcgccag
     3361 cccgagctcg tgggccctgg tcgtcacggt ggccgcgacc gggccggtga gaccccagac
     3421 gttcgggtgc agcgcggcgt gtccccggtc ggcggcgagc gtcacggcgg cgaggggctc
     3481 gaagccccgc agggtgagca gcgcggtcgg ggcggcgtcg gggacctgac ggaaccggtc
     3541 gacggtgttg aggttgaacg acgagaccag gacatcgtcg gtgtggcccc ggcgggccag
     3601 ggtctcggcg agcaacccga cgatgcgatc gtcggcgtcg aagtcggggt ctccgggcag
     3661 gttcttgacc tcgacgttga cgagcatccc gacgcaggcg tcgagcgcct cgtcgagggt
     3721 cgggatctcc gggcggctgg cctgcaccgc ggtgaaggga gcttgggcca agacaccgac
     3781 gcctcgagcc ttcgcgtcgt ggtggacgac cagcccgccg tccgagcagc ggcgcacgtc
     3841 gagctcgacc ccgtcggccc cgaggtcccg ggcccgatgg aaggcggcga cggtgttctc
     3901 gggggcttga cgccgagcgc cgcggtgagc ccagacccgg gtattcggcg acgctccggg
     3961 caccctggta tcgtaggttc tgcgcgaacg ggcaggtcag gggccttgtg gcgggtgtca
     4021 cacccgatct acgctcgctg gcgccccgcg ggtcgtgacc ccgggtcggg cgcgctggag
     4081 ccacggcccg gtagcgcggc aaccgacggc gacaaggccg aacacagcaa ggaagcagca
     4141 gtggcgctca cctggatccg gacgcatgac tgggacgccg aggcctggcg aggccgatca
     4201 gcgtgccggg actctgaccc tgacgtcttc ttccctatcg ggacgacggg ctcggct
//
LOCUS       DATCCH010000243         5060 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_525158, whole genome shotgun sequence.
ACCESSION   DATCCH010000243 DATCCH010000000
VERSION     DATCCH010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5060
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525158"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /gene="add"
                     /locus_tag="VIJ44_04780"
     CDS             <1..677
                     /gene="add"
                     /locus_tag="VIJ44_04780"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEY5165247.1"
                     /translation="DGVATALSDAQQQLGLTSRIILSFLRDLPADAAMATLDDALAYR
                     DRITAVGLDSAELGNPPEKFQAVFDRARSEGFLTVAHAGEEGPPEYIWQALDLLQVRR
                     VDHGVRCIEDERLMERLVADQIPLTVCPLSNVKLRIFPSLEQHDVGELLHRGVRVTVN
                     SDDPAYFGGYIADNYVATARALQLSTADMVQVARNSFFASFLDDADRRRHLDAIDELV
                     RAAEPG"
     gene            complement(712..1998)
                     /locus_tag="VIJ44_04785"
     CDS             complement(712..1998)
                     /locus_tag="VIJ44_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006537989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5165248.1"
                     /translation="MRAEDMILVSVDDHICEPANMFDAHVPDRYRESMPRVVDEPDGS
                     QQWYYGEVRGRNLGLNAVAGKSPEMFNVNPTRYEDMRPGCYDVHERVRDMSAGGQLAG
                     LNFPNWTGFSGQVLNLGPDRDVNLVMIKAYNDWHVDEWCGTYPDRFIPCGMVPLFDPA
                     EAAREVRRLAEKGCHAITFSENPAGLGMPSIHTDAWDPLFSACSDVDTVLCCHVGSSS
                     KSASTAPDAPPPVPMNLSSVMAIYTLGDLLWAEFWQRFPELRFALTEGDIGWIPYFLQ
                     RGEHTLNRHNAWMGHELPQGRGPTEVFRDQILCCFINDAIGVELLDHFNVDNVCWESD
                     YPHSDSTWPYAPEQLVPLVADLADDVVAKVTHANAMRHYQFDPFQTRDPSQCTVAALR
                     AEATDVDTVTRVGRRADARDVETWRRMTGGAARSKA"
     gene            2136..3299
                     /locus_tag="VIJ44_04790"
     CDS             2136..3299
                     /locus_tag="VIJ44_04790"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006347692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY5165249.1"
                     /translation="MTIDGWTAPDFDGVRHAFEKNFANGLEIGAAFAAYHHGEKVVDL
                     WGGVADPTTGQPWVHDTLELVFSTTKGAAAICANRLAQEGRLDVDAPVVDYWPEFGQA
                     GKERIPVSFLLSHQAGLAWVDEPLTLEQALAWDPMIHALERQRPHWEPGTAQGYHAVT
                     YGYLVGEVVRRITGCTLGTYFHDEIAAPLELDFWIGLPENEEPRVARLVGAVVGFDTD
                     LDESAQAELSSLLGPDSMLGKALSPGGAFSKRGIWNDRPVHAAEIPAAAGIGDARSVA
                     RMYAACVGSVDGVRLLTPAQLRDATTQRTHGPNVVLFNLDLQFGLGFIVPSTLIRLGG
                     PRSFGHFGMGGSVGWADPDAEFAFGYVMNKMDLGVTGDARSYSLIDACYAALR"
     gene            complement(3357..4757)
                     /locus_tag="VIJ44_04795"
     CDS             complement(3357..4757)
                     /locus_tag="VIJ44_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165250.1"
                     /translation="MYIAVVAAVLVALGYFPARLLTRTSTAAYLASPLMAGLVVTVLS
                     LVCVLAGLSMRLAVIGAVVVAIGSGVRVVRRRSRRRTRAESASPSVLLLPAGGLVAFA
                     TVLRPPVAWDARSIWWFHAKWFAQGGAAVVDAMRNPALSFSHPDYPPFAPATNAAAWL
                     VDGTQSAKHAQIVTALLTLSAISVLGYAVWRLFAPRLGVRTATAAGCMVVAASYGIAR
                     VDPATNGYVDVLWAASFAAACVFLLASPKSEEALRWGVLMVAVAALTKNEGLVAASFL
                     SALTAVRYGWHRRILAWLSLGLAPGLAWVAMIRVLGVVSESADQSRSGRLFALDPAVT
                     SRIGPAARSVADQVGLIAVVAAGLSLAGFSLVGPLRRRLSGSLVPWMWFAWLGTVGAL
                     VFAYVVSSRPLAWHLRTSVTRTTIAPNLVLLAEGVIWTLTIAVSIRSDPRSRTEVESR
                     PRDEPVKASPPRVGVS"
     gene            complement(4757..>5060)
                     /locus_tag="VIJ44_04800"
     CDS             complement(4757..>5060)
                     /locus_tag="VIJ44_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165251.1"
                     /translation="AGAAQGVSLAGQLLQPQAVRQLDERDRTFQCVQDRVERAVPAGS
                     TVFVEPDALLWDQRVIESTYPRLRVTSVRADADYVVRVTSSPSTCGTADVTVQRLQ"
BASE COUNT          872 a         1773 c         1671 g          744 t
ORIGIN      
        1 tcgacggcgt cgccaccgcg ctttccgacg cgcagcagca gctcggcctc acgtctcgga
       61 tcatcctctc cttcttacgg gacctgccgg ccgacgcggc gatggcgacc ctcgacgacg
      121 ccctggccta ccgggaccgc atcaccgccg tcggcctcga ttcagcagag ctcggcaacc
      181 cccccgagaa gttccaggcc gtcttcgacc gggcccgatc cgaaggcttc ttgaccgtcg
      241 cccacgcggg agaagaaggc ccgcccgagt acatctggca ggccctggat ctcctccagg
      301 tccgccgggt cgaccacggg gtccgctgca tcgaggatga gcggctcatg gagcgcctgg
      361 tcgccgacca gatcccgctc accgtgtgtc cgctctccaa cgtgaagctg cggatctttc
      421 cctcactcga gcagcacgac gtcggagagc tcctccaccg gggggtgcgc gtcaccgtga
      481 actccgatga cccggcctac ttcggcggct acatcgccga caactacgtc gccaccgctc
      541 gggcccttca gctctccacg gcggacatgg tccaggtggc ccggaactcg ttcttcgcgt
      601 cgttcctcga cgacgccgat cggcgacgcc atctcgatgc gatcgacgag ctcgtccgcg
      661 ccgcggagcc aggctgacgc tcgtcggtgc gtcgcccgtc gaccgggcgt ctcaagcctt
      721 cgacctcgcg gcgccgccgg tcatgcgccg ccaggtctcc acatcccggg cgtcggcccg
      781 ccgaccgacc cgggtcacgg tgtcgacatc ggtggcctcg gcccgcagcg cggcaacggt
      841 gcattggctc ggatcccgag tctggaacgg gtcgaactgg tagtggcgca tcgcgttcgc
      901 gtgggtcacc ttcgccacga catcgtcggc gaggtcagcg acgagcggga ccagctgttc
      961 gggggcgtac ggccaggtgc tgtcggagtg tggatagtcc gattcccagc agacgttgtc
     1021 aacgttgaag tggtcaagaa gctcgacacc gatggcgtca ttgatgaagc aacagaggat
     1081 ctggtcccgg aacacctcgg ttggtccacg cccctggggc aactcgtgac ccatccaggc
     1141 gttgtggcga ttgagggtgt gttcccctcg ctgcaggaaa tacgggatcc agccgatgtc
     1201 gccttcggtg agtgcgaacc gcagctccgg gaaccgctgc cagaactcgg cccagagcag
     1261 atctcccagc gtgtagatcg ccataaccga tgagaggttc atcggcaccg gcggaggcgc
     1321 gtccggcgcg gtggacgcgc tcttggagga cgatccgacg tggcagcaca ggacagtgtc
     1381 cacgtccgaa cacgccgaga agagcggatc ccacgcatcg gtatggatgc tcggcatccc
     1441 gagcccggcc gggttctccg agaacgtgat cgcgtgacag cccttctccg ccaggcgtcg
     1501 aacctcccgc gccgcctccg cgggatcgaa gagcggaacc atgccgcacg ggatgaaccg
     1561 gtcggggtac gtgccgcacc attcgtcgac atgccagtcg ttgtaggcct tgatcatgac
     1621 aaggttgacg tcacggtcgg gaccgagatt caggacttgt ccggagaaac cggtccagtt
     1681 cgggaagttc agccccgcca gctgcccacc ggctgacatg tcgcgtaccc gctcgtggac
     1741 gtcgtaacac cctgggcgca tgtcttcgta ccgggtgggg ttgacgttga acatctcggg
     1801 cgatttgccc gccaccgcgt tcaagccgag gttccggcct cggacctcgc cgtagtacca
     1861 ctgttgtgat ccgtcgggct catcgacgac tctcggcatc gactcgcggt aacgatcggg
     1921 gacatgcgcg tcgaacatgt tggccggctc gcagatgtga tcgtcgaccg agaccaggat
     1981 catgtcctcg gcacgcactc gcacctcccg tgaccacacg agcctactgg cgccacccgc
     2041 gaagccgccc gagccgccca agcaacctct cggatcgacg tgtgggccac cgcgcccctg
     2101 gcgatccgat cgacgcgctg ctaggaagtc cgaccgtgac gatcgacgga tggaccgcac
     2161 ccgacttcga cggggtccgg cacgcctttg agaagaactt cgcgaacggt ctcgagatcg
     2221 gagccgcgtt cgcggcatac caccacggtg agaaggtcgt cgatctctgg ggcggtgtcg
     2281 ccgacccgac gaccggacaa ccctgggtgc acgacacgct ggagctggtg ttctccacca
     2341 cgaagggcgc ggccgcgatc tgcgccaacc gcctcgcaca agaaggccgc ctcgacgtcg
     2401 acgcccccgt ggtcgactac tggcccgagt tcggccaggc cggcaaggag cggatcccgg
     2461 tgagcttcct gctgtcccat caggccgggc tggcctgggt ggacgagccg ctcaccctcg
     2521 agcaggcgtt ggcctgggat ccgatgatcc acgctctcga gcgccagcgg ccgcactggg
     2581 agcccggcac cgcgcagggc tatcacgccg tcacgtacgg ctacctggtc ggcgaggtgg
     2641 tccggcgcat caccggctgc acgctcggca cctactttca cgacgagatc gccgccccgc
     2701 tcgaactcga tttctggatc ggtctgcccg agaacgagga accccgagtg gctcggctcg
     2761 tcggcgcggt cgtgggattc gacaccgacc tcgacgagtc cgcccaggcc gagctctcct
     2821 ccctgctcgg tcccgactcg atgctcggaa aggcgttgtc ccccggcggc gcgttcagca
     2881 agcggggcat ctggaacgat cgtccggtcc acgccgcgga gatcccggcc gcggcgggca
     2941 tcggagacgc gcggtcggtg gcgcggatgt acgcggcctg cgtcggatcc gtcgacggtg
     3001 ttcgcctgct cacaccggcg cagctgcgcg acgcgacgac tcagcgcacc cacggcccca
     3061 acgtcgtgct cttcaacctc gacctgcagt tcggcctggg ttttatcgtc ccgtcgacct
     3121 tgatccgcct gggtggcccc cgctccttcg gccacttcgg catgggggga tcggtgggct
     3181 gggccgatcc cgacgccgag ttcgcgttcg gatacgtcat gaacaagatg gacctcggcg
     3241 tgacgggcga cgcacgcagc tacagcctca tcgacgcctg ctacgccgcc cttcgctgat
     3301 cgacacgctc ggcggctcga gacgaccggc ccgcgcaccg ctccgatgcg gtgtggtcag
     3361 gacaccccga cccggggcgg cgaagccttc accggctcgt cgcggggtcg ggactcgacc
     3421 tcggtgcggg agcgagggtc ggaccgaatc gacaccgcga tggtcagcgt ccagatcacg
     3481 ccctccgcga gcagcaccag attcggggcg atggtggtgc gggtgaccga ggtccggaga
     3541 tgccacgcca ggggacggct gctgacgacg taggcgaaca caagggcacc gaccgtgccc
     3601 agccacgcga accacatcca cgggacgagc gacccgctga gccggcgccg caacggaccc
     3661 acgagagaga agccagcgag cgaaagcccc gcggccacca ccgcgatcag accgacctga
     3721 tccgcgaccg aacgagccgc cggtccgatc cgactggtca cggcagggtc gagcgcgaag
     3781 agacgcccgc tccgtgactg gtccgccgac tctgacacga caccgaggac ccggatcatg
     3841 gcgacccagg ccaagcccgg agccaagccc aggctgagcc aggccaggat ccgtcggtgc
     3901 cacccgtacc ggaccgccgt caacgccgag aggaacgacg ccgcgacgag cccttcgttc
     3961 ttggtgagcg ccgccaccgc caccatgagc acaccccacc gcagggcctc ctccgacttg
     4021 ggggacgcga gcaggaaaac acacgccgcc gcgaacgatg ccgcccacag cacgtccaca
     4081 tacccgttgg tggccggatc gacccgcgcg attccgtagg aggcggcgac gaccatgcag
     4141 cccgcggcgg tcgcggttcg aacaccgagt cgaggggcga acagtcgcca gaccgcatag
     4201 ccgagcaccg agatcgcgct gagggtgagc agcgccgtca cgatctgggc gtgcttggct
     4261 gactgggtcc cgtcaaccag ccaggcggcg gcgttcgttg ccggggcgaa gggcggatag
     4321 tcaggatgag agaacgagag agccgggttg cgcatcgcgt cgaccaccgc ggcgccaccc
     4381 tgcgcgaacc acttggcgtg gaaccaccag atcgaccggg catcccaggc gactgggggt
     4441 cgaaggacgg tcgcgaacgc gacgagtccg ccggcgggca gcaggagcac gctcggcgaa
     4501 gccgattccg cgcgagtccg ccgccgggat cgacgacgga ccacgcgtac gccactgccg
     4561 atcgccacga ccacggctcc aatcacggcg agacgcatcg acaggccggc caggacgcac
     4621 accaggctga ggacggtgac gacgagtcca gccatcaggg ggctggcgag atacgcggcg
     4681 gtgctcgtgc gtgtgagcag ccgagccggg aagtacccga gcgcgaccag gaccgcggcc
     4741 acgacggcga tgtacatcac tgcaacctct gaaccgtcac gtcggcagtt ccgcaggtcg
     4801 aaggcgagct ggttactcga acgacgtagt cggcgtcggc tcgcaccgac gtcactcgca
     4861 ggcgcggata cgtcgattcg atgacccgct ggtcccacag cagcgcgtcg ggttccacaa
     4921 agacggtgga gcccgccggc acagcccgtt cgacccggtc ctggacacac tggaaggtcc
     4981 ggtcgcgttc atcgagttgg cgaaccgctt ggggctgcag gagctggccc gcgagcgaga
     5041 caccctgtgc cgcgccggcg
//
LOCUS       DATCCH010000244         2103 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_525254, whole genome shotgun sequence.
ACCESSION   DATCCH010000244 DATCCH010000000
VERSION     DATCCH010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525254"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..554
                     /locus_tag="VIJ44_04805"
     CDS             81..554
                     /locus_tag="VIJ44_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165252.1"
                     /translation="MLLIPKTTGSTVPLPVKVRLPVALAVGPIVVVGAGAPSKPSVTV
                     AVDTVVFVTGAAVEPDSRTLVGVPGCVVSVVEVVDVDVEVDVDVEVEVDVDVDVEVDD
                     VVVVGPQLPILGSEADAEKSCDRSGLRMINLTVPLDGAGPLACQICTGPEFPVVS"
     gene            1343..1552
                     /locus_tag="VIJ44_04810"
     CDS             1343..1552
                     /locus_tag="VIJ44_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165253.1"
                     /translation="MRATVAGHVLAVVACVFAVLCFEHLDHTVALAVVAVAAAIGLIA
                     SARRVPLALWWTLGMVIGGALGRWS"
     gene            1623..2000
                     /locus_tag="VIJ44_04815"
     CDS             1623..2000
                     /locus_tag="VIJ44_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020459848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEY5165254.1"
                     /translation="MAQSAGLHEPEERLSPETIDRHRALVSIQEELEAVDWYAQRVDA
                     ATDAELAAVLAHNRDEEKEHAAMAIEWLRRHDPALDRHLRTYLFTSGSITEIETEAEA
                     TDGADAPAPSGSLGIGGLKGAAR"
BASE COUNT          328 a          684 c          738 g          353 t
ORIGIN      
        1 gggaagagcg cggggtgttg cgcgcagctg tgcgctagca ggtgctccgt ttgcgcagga
       61 ggagcaatgc tccggcggcg atgctgctca tcccgaagac gacggggagc accgttccgc
      121 tgccggtgaa ggtaaggctc ccggtggcgc tggcggtcgg cccgatcgtc gtcgtcgggg
      181 ccggagctcc cagcaaaccg agcgtcacgg tggccgtcga cacggtggtc ttcgttaccg
      241 gtgccgccgt cgagcccgac tctcggacac tcgtcggggt gcccggctgc gtcgtgtcgg
      301 tcgtcgaggt ggtcgacgta gatgtcgagg tcgacgtaga tgtcgaggtc gaggtcgacg
      361 tggacgtgga cgtcgaggtc gacgacgtag tggttgtggg cccgcagtta ccgatcctgg
      421 gcagcgaagc cgacgccgag aagtcgtgcg accggtccgg cttgaggatg atcaacttga
      481 cggtgccgct ggatggcgcg gggccactgg cgtgccagat ctgcaccggg cccgaattcc
      541 ctgtcgtgtc gtagtgaccg tcgacgatgc cggtcgttcc cgggatggtc gacgtcacct
      601 cgacgaagtg gtcgggggtg acgttgttct cgagggtcac cgaggcagac caacgcatgc
      661 acgtctgcga cgtgacgacg tgtccggtgt tcgcaccggc cgggagcgcg aagccgaaca
      721 cggccgcaac cgccactcct gccacgattc ccactcggcc cgcacgggta tgcacgattc
      781 ccagcacacg gttcacaggt gactcccagc gagttcccag ctattaggct atcgtccgat
      841 acgtcaagtt aagcacattt ccgccatttg tgtgcggact gtaggcattt gtgtgctggc
      901 tgtgacaaat gtcacttcgt cgcgcgacgg gcgaaacgct caccgcgagg ttcttcgggt
      961 cgggggcggt catcgtcgcc tcgcgcacgt tcgccccgag cccccgggac gataatgaga
     1021 cgtggaggaa tcgcccgagc aggtcttggc gcggctggct cgataccgcg actcgttgaa
     1081 cgggaggccg cgatcgccga tcgccaaccc ggcgcgctcg ccggcgcggg cgccgacgac
     1141 gaccaccacc accacgcttc cggccccgag acctcccaca gggcttccca cgggaattct
     1201 caccagacct cacccggcgc ctcggcccgc tccagtcgcg gcttcgggaa ccggggacag
     1261 cgccgccgga gcgttcgcgc gcgggtcggc gcgcgcctgg gactactcga ccgcgaccgc
     1321 gcgggcggtg accctgagtg tcgtgcgggc gacggtcgcc ggccatgtcc tggccgtggt
     1381 cgcatgcgtg ttcgcggtgc tctgtttcga gcatctcgat cacacggtcg cgttggcggt
     1441 ggtcgccgtc gcggccgcga tcggcttgat cgcgagcgcc cggcgcgtcc cgctcgccct
     1501 gtggtggacg ctcgggatgg tgatcggagg cgcgctcgga cgctggtcct gacggcctcc
     1561 gacccgcaca agttctcgcg ccggtgttgt agtgggcttc gcggacgggt aaaacccacc
     1621 gcatggccca gagcgcggga ttgcacgaac ccgaagaacg gttgtccccg gagaccatcg
     1681 accgccatcg ggcgctcgtc tccatacaag aagagctgga ggcggtcgat tggtacgcac
     1741 agcgagtcga cgccgctact gacgcggagc tggctgcggt ccttgcgcac aatcgcgacg
     1801 aggagaagga gcacgcagcc atggcgatcg aatggttgcg ccgccacgat cccgcgttgg
     1861 accgtcacct ccgtacgtac ctcttcacct ccggatcgat taccgagatc gagaccgaag
     1921 cggaggcgac cgacggtgcg gacgcgccgg cgccgtcggg ctccctggga atcggcgggc
     1981 tgaaaggagc ggcgagatga accacctcct ccgctcgtat gcgcccatca gtgattccgg
     2041 ctgggacgag ctcgaccgcg aagttcgaca acggctgctg ccgggactgg ccgcgcgccg
     2101 gct
//
LOCUS       DATCCH010000245         3185 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_193367, whole genome shotgun sequence.
ACCESSION   DATCCH010000245 DATCCH010000000
VERSION     DATCCH010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193367"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="VIJ44_04820"
     CDS             <1..771
                     /locus_tag="VIJ44_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165255.1"
                     /translation="IPMADWILIGHSHFDHLWGAERIACNTGATVVGSYETVRVLLAA
                     GIPARQLIPVAGGERIQLAPHVVVSVHPSQHSCVWAHRQFPGADTACLGDLGLTHQER
                     QVRFAELGAWLVGLGPEVLKHLRASDQHALGDGGALVFVLDTPAGRLLYQDTSGCWTP
                     ILRDLHPDVAILAAAGRGNLDGEPIQGSLAQFVGAEVRLLGPRRLVLAHHDDWLPGFT
                     SGDLDVTPIRHELARTSPDTTLVELDYTSASDVLTSFR"
     gene            855..940
                     /locus_tag="VIJ44_04825"
     tRNA            855..940
                     /locus_tag="VIJ44_04825"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:891..893,aa:Ser,seq:tga)
     gene            complement(956..2200)
                     /gene="serS"
                     /locus_tag="VIJ44_04830"
     CDS             complement(956..2200)
                     /gene="serS"
                     /locus_tag="VIJ44_04830"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEY5165256.1"
                     /translation="MIDLRQLREDPEYRAGIKRKRVAPGLIGEVLALDDEERAQRRTV
                     EAVRAEQKAVSKEIARDPAGRDAKLAEASSLKDQVQQAEQELGVLERRLRDLALQLPN
                     PADPTVPDGGEDDGEVLRTIGDPGPAPSRDHAAFADAMRFVETRHAAESSGSRFASLL
                     GEAVLVDLALVNWTMARLAAEGFTPVIPPVLVRESAMEEAGFFPTDRAQVYDVDRGAL
                     YLVGTSEIPLSTLHRGERLDPSALPARYAGLSTCFRREAGTHGKDTSGIFRVHQFDKV
                     EMFSYTDPDQSDEEHERILSIQESLIGALGLPYRVVNIAAGDLGAAAAKKYDLEVWLP
                     SEGRYRELTSSSNYRDFSARRLGTRVKSDQGTRLVHTLNGTGCAVGRTLVFLWEHYQR
                     DDTTFDVPDVLRPYCGFDSVSR"
     gene            complement(2211..>3185)
                     /gene="pheA"
                     /locus_tag="VIJ44_04835"
     CDS             complement(2211..>3185)
                     /gene="pheA"
                     /locus_tag="VIJ44_04835"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012850512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEY5165257.1"
                     /translation="KRRRVDEDPPLRSIVVTQVGYLGPRGTFAEEALLSQPDLAAGTA
                     VPLRTVPAVLTAVERGEVDVGLVPIENSIEGTVTVTLDTLAFDTELLVQREVDLPVSL
                     HLCAQPGTGLDAIRTVVSHPNPFGQCRTWLTRKLPDAELEAANSTADAARHVVASERD
                     DVASIGTAHAARLYGLEILASDIEDHPENETRFVLVGRGIPAPTGHDKTSIVCFQRED
                     RPGSLLAILQEFAARAINLTKLESRPTKRGLGQYCFFIDFEGHLADELVADCLRNLAA
                     RQADVKFLGSYPVAGEAGPARREAASEAWREASGWVDGLRAQIRSDGP"
BASE COUNT          450 a         1083 c         1142 g          510 t
ORIGIN      
        1 atccctatgg ccgactggat cctgatcggc cattcgcatt tcgatcatct ctggggcgcg
       61 gagcgcatcg cgtgcaacac cggggccacc gtcgtgggct cttacgagac ggtccgggtg
      121 ctcctcgccg ccggcatccc ggcccgccag ctgatcccgg tagcgggcgg agagcgaatc
      181 cagctcgctc cccacgtcgt cgtgtccgtg cacccaagtc agcactcctg tgtctgggcc
      241 caccggcagt tcccgggcgc cgacacggcg tgcctcggcg atctcggcct cacccaccaa
      301 gagcgccaag tccgcttcgc ggagctcgga gcgtggctcg tcgggctcgg acccgaggtg
      361 ctcaagcatc tccgggccag cgaccagcac gccctggggg acggcggcgc gctggtcttc
      421 gtgctcgaca cccccgccgg ccggctcctc taccaggaca cgtcaggctg ttggacgccg
      481 atcttgcggg acctgcaccc cgacgtggcg atcctcgccg ccgcgggccg cggcaacctc
      541 gacggcgagc cgatccaggg gtcgttggct cagttcgtgg gagccgaggt gcggctcctc
      601 gggccgcgcc ggctggtgct ggcgcaccat gacgactggc tccccgggtt caccagcggt
      661 gacctcgacg tcaccccgat ccgccacgag ctcgcccgaa cctcgcccga caccacgctg
      721 gtggagctcg actacacgtc cgcgtcggac gtgctgacct cgtttcgctg accgactccc
      781 ggcgtcaagg gcgccgccta cgatttccgg tacggcgcga cgcacggtcg acggtgtggc
      841 cggtgtcggg tcagggagcg gtggcagagt ccggttgatt gcagctgcct tgaaagcagc
      901 cggccgatga ggcccggggg ttcaaatccc tcccgctccg ccggatggcg ccacgtcagc
      961 gcgagacgga gtcgaagccg caataggggc gcagcacgtc cgggacatcg aacgtcgtgt
     1021 cgtcgcgctg gtagtgctcc cagaggaaca cgagggtgcg cccgaccgcg cagcccgtcc
     1081 cgttgagcgt gtggacgagg cgcgtgccct ggtcggactt gacgcgcgtt ccgagccgac
     1141 gggcggagaa gtcgcggtag ttggagctcg acgtcagctc gcggtaccga ccttccgagg
     1201 gcagccacac ctcgaggtcg tacttcttgg ccgcggcggc gccgaggtcc ccggcggcga
     1261 tgttcaccac ccgatacggc aagccgagcg cgccgatcag cgattcctgg atcgagagga
     1321 tccgctcgtg ttcctcgtcg gactggtcgg gatcggtgta ggagaacatc tcgaccttgt
     1381 cgaactggtg gacccggaag attccgctgg tgtccttgcc gtgcgttcct gcctcccggc
     1441 gaaaacaggt cgagaggccg gcatagcgcg ccgggagcgc gctcgggtcg agccgctcgc
     1501 cccgatgcag ggtggagagc gggatctcgc tggtgccgac gaggtacagc gcgccgcggt
     1561 cgacgtcgta gacctgggcc cggtcggtcg ggaagaaccc ggcctcttcc atcgcgctct
     1621 cgcgtaccag caccggcggg atgacgggcg tgaacccttc ggcggcgagg cgggccatgg
     1681 tccagttgac gagggcgagg tcgacgagga cggcttcgcc gagcagcgac gcgaacctcg
     1741 agccgctcga ctccgctgcg tggcgggtct cgacgaacct catcgcgtcg gcgaacgcgg
     1801 cgtggtcccg gcttggcgcc gggccggggt cgccgatggt gcgcagcacc tcaccgtcgt
     1861 cttcgccccc gtccgggacg gtggggtcgg ccgggttggg gagctgcagg gccaggtccc
     1921 gcagccgccg ttcgaggacg ccgagctcct gctcggcctg ctgcacctgg tccttgaggc
     1981 tgctcgcttc ggccagcttg gcgtcccggc cggcggggtc ccgggcgatc tccttcgaca
     2041 ccgccttctg ctcggcgcgg acggcctcga ccgtccgtcg ttgggcgcgc tcctcgtcgt
     2101 cgagggccag gacctcgccg atgagcccgg gagcgacccg tttgcgtttg atcccggcgc
     2161 ggtactcggg gtcctctcgg agttgtcgaa ggtcgatcac ggccgtgggg tcacggcccg
     2221 tcggatcgga tctgggctcg cagcccatcc acccatccgc tcgcctcccg ccacgcctcc
     2281 gatgccgcct cccgccgggc gggaccagct tcaccagcga cagggtagga gccgagaaac
     2341 ttcacgtcgg cttgccgggc cgccaggttg cgcaggcagt cggcgacgag ctcgtcggcg
     2401 agatgcccct cgaagtcgat gaagaagcag tactggccga gtccgcgctt ggtcggccgc
     2461 gattcgagct tggtgaggtt gatggcgcga gcggcgaact cttggaggat ggcgagcagg
     2521 gaaccggggc ggtcctcgcg ctggaagcac acgatggacg tcttgtcgtg accggtgggg
     2581 gcagggattc cgcgaccgac gagcacgaag cgggtctcgt tctcggggtg atcctcgatg
     2641 tcggaggcga ggatctcgag cccgtagagg cgagccgcgt gggccgtccc gatcgacgcc
     2701 acgtcgtctc gctccgacgc gacgacgtgg cgggcggcgt cggcggtgga gttcgcggcc
     2761 tcgagctcgg cgtcgggaag cttgcgggtg agccaggtac ggcactgccc gaaggggttg
     2821 gggtgagaga cgacggtgcg gatcgcgtcg agacccgttc ccggctgggc gcacaggtgc
     2881 agcgacacgg gaaggtcgac ctcgcgttgg accagcagct cggtgtcgaa ggccaacgtg
     2941 tcgagcgtga cggtcaccgt gccctcgatc gagttctcga tcggcacgag cccgacgtcc
     3001 acctcgcctc gctcgaccgc ggtgagcacc gcgggaacgg tccgcaacgg aactgccgtc
     3061 cccgccgcga gatcaggttg gctgagcaac gcctcctcgg cgaacgtgcc acgcggtccg
     3121 agatagccca cctgagtcac gacgatcgat cgtagtggtg ggtcttcgtc gacccggcga
     3181 cgttt
//
LOCUS       DATCCH010000246         2116 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_193374, whole genome shotgun sequence.
ACCESSION   DATCCH010000246 DATCCH010000000
VERSION     DATCCH010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193374"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VIJ44_04840"
     CDS             <1..229
                     /locus_tag="VIJ44_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165258.1"
                     /translation="ASDAVVRVVGRYAGERSAGETFPDWLGRSGGATAVGEGLRDLDA
                     FPDPEEAPEFYIDFDETGPYVKDVGDSECAT"
     gene            217..897
                     /locus_tag="VIJ44_04845"
     CDS             217..897
                     /locus_tag="VIJ44_04845"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEY5165259.1"
                     /translation="MRDVNDRDLPDLRQLDAASTALESQPATAAIEWAAERFGDGLVL
                     ASSFQDCVLIDLAAQVVPDIEVVFLDTQYHFAETLWYVETVRRRYDLNLRIVEPDVTP
                     DDRWLDDPDGCCAARKVVPMAKALAGRTAWMTGLRRDEAPTRTKTPVVGYDVGRGMVK
                     INPIADWTEIDVQRYAQDRSLPEHPLREKGYASIGCWPCTRPVAEGEDARAGRWGGLD
                     KLECGLHR"
     gene            933..1700
                     /locus_tag="VIJ44_04850"
     CDS             933..1700
                     /locus_tag="VIJ44_04850"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY5165260.1"
                     /translation="MGIHDVAARGFDAGADVYERARPSYPPDAVACLVEHLRIGPGTT
                     VVDLAAGTGKLTRLLAPTGASLLAVEPVDGMRAQLRATQPEIPVTAATAEALPFRDAT
                     LDAVTVAQAFHWFDAERAFTELARVLRPGGGVGMIWNARDRRVDWVNQLWSIMDRVEK
                     RAPWRDHDRWSDAALGARAGFGPLHEATFQHEQVISADGIVERFASVSHVAVLPDAER
                     GAVLDEIRAFLATSPETAGRDDLALPYRVDAYWCERR"
     gene            1697..>2116
                     /locus_tag="VIJ44_04855"
     CDS             1697..>2116
                     /locus_tag="VIJ44_04855"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEY5165261.1"
                     /translation="MTRSLRTRLGVRAGRIAGWLSRVTGRGAGATISGRVINAVAPDA
                     LAELAGGRRTALVSATNGKTTTTRLLAAAVATNGRSVTTNSTGANLTSGLAPTLARDP
                     DADTAILEVDERVVSRVVDPLRAELLVLGNLSRDQLDR"
BASE COUNT          303 a          812 c          730 g          271 t
ORIGIN      
        1 ggcatcggac gcggtggtgc gggtggtcgg gcgctacgcc ggtgagcgca gcgccggcga
       61 gacgtttccc gactggctcg gacgctccgg tggcgccacc gccgtcgggg agggcttgcg
      121 tgacctcgac gcgttcccgg accccgagga agcacccgag ttctacatcg acttcgacga
      181 gaccggaccg tacgtgaagg acgtcggtga cagcgagtgc gcgacgtgaa cgaccgcgac
      241 ctccccgacc tgcgccagct cgacgcggcg tcgaccgccc tcgaatccca gcccgcgacg
      301 gcggcgatcg aatgggccgc ggagcgcttc ggtgacgggc ttgtcctggc gtcgtcgttc
      361 caggactgcg tcctcatcga cctggcggcg caggtggtgc ccgacatcga ggtcgtgttc
      421 ctcgacaccc agtaccactt cgccgagacg ctctggtacg tcgagaccgt ccggcgccgc
      481 tacgacctga acctgcggat cgtcgaaccc gacgtgaccc ccgacgaccg gtggctcgac
      541 gaccccgacg ggtgctgcgc cgcccgcaag gtcgtcccga tggcgaaggc gctcgcggga
      601 cgcaccgcgt ggatgaccgg gctgcgccgc gacgaggcgc ccacccggac caagaccccc
      661 gtcgtcggct acgacgtcgg gcgcggcatg gtgaagatca accccatcgc cgactggacc
      721 gagatcgacg tccagcggta cgcccaggac cgctccctcc ccgagcaccc gttgcgcgag
      781 aagggctacg cgtcgatcgg gtgctggccc tgcacccgac cggtcgccga gggcgaagac
      841 gcccgcgccg gacgctgggg cgggctcgac aagctggagt gcggcctcca ccgctgaccc
      901 gcagccaacc ggcggggtgc caacatcaag ccatgggaat ccacgatgtc gccgcgcgcg
      961 gcttcgacgc tggcgctgac gtctacgaac gggcccgacc gtcctacccg cctgacgcgg
     1021 tggcgtgtct cgtcgagcac ctccgcatcg ggcccgggac gaccgtcgtc gacctcgccg
     1081 ccggcaccgg gaagctgacc cggctcctcg ccccgaccgg cgcctcgctc ctcgcggtgg
     1141 agccggtcga cgggatgcga gcccagctcc gcgccaccca acccgagatc ccggtcaccg
     1201 ccgccaccgc ggaggcactg ccgttccgtg acgccacgct cgacgcggtg acggtggcgc
     1261 aggccttcca ctggttcgac gccgagcggg cgttcaccga gctggcccgg gtgctccgac
     1321 ccggcggggg cgtcgggatg atctggaacg cccgcgaccg ccgggtcgac tgggtcaacc
     1381 agctgtggtc gatcatggac cgggtcgaga agcgcgcccc gtggcgggat cacgaccggt
     1441 ggagtgacgc cgcgctcggc gcccgggccg gcttcggtcc gctgcacgaa gcgacgttcc
     1501 agcacgagca ggtcatcagc gccgacggga tcgtggagcg gttcgcgtcg gtgagccacg
     1561 tcgcggtcct gcccgacgcc gaacgcggcg cggtcctcga cgagatccgg gcgttcctcg
     1621 ccaccagccc cgagaccgcc ggtcgtgacg acctcgcgct cccctaccgg gtcgacgcgt
     1681 actggtgcga gcgccgatga cgcgatccct ccgcacccgg ctcggagtcc gagccggtcg
     1741 gatcgccggc tggctctcac gcgtcacggg acgcggcgcg ggcgcgacca tcagcggccg
     1801 ggtcatcaac gccgtcgccc ccgacgcgct cgccgagctc gccgggggtc ggcgcaccgc
     1861 gctggtgtcc gccaccaacg gcaagaccac caccacccgg ctcctcgccg ccgcggtagc
     1921 gaccaacggc cgctcggtga cgacgaactc gaccggcgcc aacctcacct cgggactcgc
     1981 gcccacgctg gcccgtgacc ccgacgccga caccgcgatc ctcgaggtcg acgagcgcgt
     2041 cgtgtcccgc gtcgtcgacc cgcttcgcgc cgagctgctc gtcctcggca acctgagccg
     2101 cgaccagctc gaccgg
//
LOCUS       DATCCH010000247         2710 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_110362, whole genome shotgun sequence.
ACCESSION   DATCCH010000247 DATCCH010000000
VERSION     DATCCH010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110362"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="VIJ44_04860"
     CDS             complement(<1..686)
                     /locus_tag="VIJ44_04860"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006538275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY5165262.1"
                     /translation="MEFGIFNSLFTPHQAYEAEADQWAVEAQRLRNEVTWTKAADRAG
                     FKYSWATEHHFLTEYSHLSSNEAFLGYLAGVTERIHLGSGIFNITPPVSHPARVAEKV
                     AMLDHLSGGRFEFGVGRGSSSTEQRGFGIMNPEETKAMVDEVLPEFKKMWRENDYSFR
                     GKYFSLPPRNVLPKPFSDPHPPMWIAAGNPGTFEKAARLGLGVLCFTTGSPETLKPLI
                     EIYKNSIDKA"
     gene            complement(755..1213)
                     /locus_tag="VIJ44_04865"
     CDS             complement(755..1213)
                     /locus_tag="VIJ44_04865"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY5165263.1"
                     /translation="MGFTRAEIEDAFQHFQEAADRSAKSGEWREWSECFTDDADYYEH
                     HYGKMHGRQAIYEWIQSTMSDPSVSDMRNFPIDWYVIDEDRGWVLCSVWNEMDDPGDG
                     SLHREYNWTILHYAGDGLFSYEEDMYNPVEFGRMVSGWHDAKRRLAEDQN"
     gene            1369..2208
                     /locus_tag="VIJ44_04870"
     CDS             1369..2208
                     /locus_tag="VIJ44_04870"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5165264.1"
                     /translation="MRVAYVGLGNMGAPMAQNARRAGHDLTVFDVRVDAMTPLAELGA
                     RPCGSAAEAVAGVDVVCVTVLDGSQVDAAMRGADGVFGGVAPETVVAIHSTVHPQTIH
                     AIAGQAPAGVEVLDAPISGGVKGARAATLCVMVGGPADAFERARPVFDAVGDLVVHLG
                     DRGAGLAAKLARNLVGYVTMLAAQEGRQLAAAAGTDLERLNEILEHTGALSPMMRDLL
                     SVPGGDDVYSEDVAPLVALAAKDLRATLAFGADLGVDLPAAALTLDRVAFSFGVTDQD
                     TGA"
     gene            2205..>2710
                     /locus_tag="VIJ44_04875"
     CDS             2205..>2710
                     /locus_tag="VIJ44_04875"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005632546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEY5165265.1"
                     /translation="MKVSLGLPTHQVGGELAAADGIAEVSVAAETAGFDAVFVTDHPF
                     PSDRWLATGGHHALDPFVALSFAAAATTRLRLHTNLLVLAYRNPFHSAKAIASLDVLS
                     NGRVIVGVGAGYLEAEFEALGVDFDARNDLTDDAIAAMRTAWTGESVAAAGRGWRAPG
                     NTMQPVPT"
BASE COUNT          406 a          938 c          907 g          459 t
ORIGIN      
        1 tcggccttgt cgatggagtt cttgtagatc tcaatgagcg gcttgagggt ctcgggtgac
       61 ccggtggtga agcagagcac cccgagcccg agccgggccg ccttctcgaa ggtgccgggg
      121 ttcccggccg cgatccacat cggcgggtgc gggtcactga acggcttcgg tagcacgttg
      181 cggggcggga gcgagaagta cttgccccgg aagctgtagt cgttctcccg ccacatcttc
      241 ttgaactcgg gcagcacctc gtcgaccatc gccttcgtct cctcggggtt catgatcccg
      301 aagccgcgct gctccgtcga cgaggagcct cggcccaccc cgaactcgaa ccggccgccg
      361 gagaggtggt cgagcatcgc gaccttctcg gccacccggg ccgggtgaga gaccggggga
      421 gtgatgttga agatcccgct gccgagatgg atgcgttcgg tgaccccggc gagatagccg
      481 aggaacgcct cgtttgatga caggtgcgag tactcggtca ggaagtggtg ctccgtcgcc
      541 cacgagtact tgaacccggc gcggtccgca gctttggtcc acgtcacctc gttgcggagt
      601 cgctgcgcct cgacggccca ctggtcggcc tcggcctcgt acgcctgatg cggtgtgaag
      661 aggctgttga agatgccgaa ctccatcgtt ccccctagtg gtggtggcgg gccgcgcgga
      721 tcgtaccgac gccaacccaa aagtgcaaca cgttctagtt ttggtcctcg gcgaggcgcc
      781 gcttggcgtc gtgccagcca ctgaccatcc ggccgaactc gaccgggttg tacatgtcct
      841 cctcatagga gaacagcccg tcgccggcgt agtggaggat cgtccagttg tactcacggt
      901 gcagggaacc gtcaccgggg tcgtccatct cgttccacac cgagcacagg acccagcctc
      961 ggtcctcatc gatcacatac caatcgatcg ggaaattccg catatcggag accgacgggt
     1021 cgctcatcgt cgactggatc cactcgtaga tggcctggcg cccatgcatc ttcccgtagt
     1081 gatgctcgta gtagtcggcg tcgtcggtga agcactccga ccactcgcgc cactcaccgc
     1141 tcttcgccga ccggtcggcc gcctcctgga aatgctggaa ggcgtcctcg atctccgccc
     1201 gggtgaaccc cacctgtgcc tcctcggtcg agcatccgag cccaccgaac cggtcgggct
     1261 ctacgatccg gcgatcgtaa acggcaagcc aggtgtcgcg acaggcctcc gtcgcccccg
     1321 aacccgcgcc ctcggcgtcg ccgctggcgc ggcgatcacg cggtgggcat gagagtcgcc
     1381 tacgtgggcc tcgggaacat gggtgcgccg atggctcaga acgcacgccg cgccggtcat
     1441 gacctcacgg tgttcgacgt tcgtgtggac gcgatgacgc cgctagccga gttgggggcc
     1501 cgaccctgcg ggtcggcggc ggaggccgtc gcgggtgtcg acgtcgtgtg tgtcaccgtg
     1561 ctcgacggct ctcaggttga cgccgccatg cgcggggcgg acggggtctt cggcggcgtg
     1621 gcacccgaaa cggtggtggc gatccacagc accgtgcacc cacagacgat ccatgcgatc
     1681 gcaggacagg caccggcggg agtggaggtg ctcgacgccc cgatcagcgg tggcgtgaag
     1741 ggggcccgag ccgcgacgtt gtgcgtcatg gtcggcggcc cggccgacgc cttcgaacgg
     1801 gcccgtccgg tgttcgatgc cgtgggcgac ctggtcgtgc acctcggcga ccgcggggcc
     1861 ggtctcgccg cgaagctcgc ccgcaacctt gtcggctacg tcacgatgct ggcagcacaa
     1921 gaaggccgtc agctcgcggc cgcggccggc accgacttgg aacgactgaa cgagatcctg
     1981 gagcacaccg gcgcgttgag tcccatgatg cgcgacctgc tctcggtccc cggcggcgac
     2041 gacgtctatt ccgaggatgt cgccccgctg gtcgcgctcg cggcgaagga cctgcgcgcc
     2101 acgctggcgt tcggcgcgga tctcggtgtc gatctccccg ccgcggcgct caccctggat
     2161 cgggtggcgt tctcgttcgg tgtcaccgat caggacaccg gagcgtgaag gtctcgctcg
     2221 ggttgccgac ccaccaggtc ggcggagagc tcgccgccgc cgacggcatc gccgaggtgt
     2281 cggtggcggc ggagacggcc ggcttcgacg ccgtgttcgt caccgaccat cccttcccgt
     2341 cggaccgctg gctcgcgacc ggcggccacc acgccctcga cccgttcgtc gccctcagct
     2401 tcgccgccgc ggccacgacc cgcctccgcc ttcacaccaa tctgctggtc ctggcctatc
     2461 ggaacccgtt tcactcggcg aaggcgatcg cctcgctcga cgtgctctcc aacggtcggg
     2521 tgatcgtcgg cgtcggcgcc gggtacctcg aggcggagtt cgaagcactc ggcgtcgact
     2581 tcgacgcccg caacgacctc accgacgacg cgattgccgc catgcgcacc gcctggaccg
     2641 gcgagagcgt cgccgccgca gggcgggggt ggcgcgcacc gggcaacacc atgcaacccg
     2701 ttccgacgca
//
LOCUS       DATCCH010000248         2600 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_387336, whole genome shotgun sequence.
ACCESSION   DATCCH010000248 DATCCH010000000
VERSION     DATCCH010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387336"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..430)
                     /locus_tag="VIJ44_04880"
     CDS             complement(86..430)
                     /locus_tag="VIJ44_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165266.1"
                     /translation="MRRSTVWRAFGLGVLAGTGYAVWRAIEASRATEVAWDPQPFPPQ
                     PRAADPSPGTTSTWVEPDNGVCPASHPVKAKLASGIYHLPGGQSYERTRPDRCYRDGG
                     AAEADGLRPAKR"
     gene            650..2191
                     /locus_tag="VIJ44_04885"
     CDS             650..2191
                     /locus_tag="VIJ44_04885"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEY5165267.1"
                     /translation="MTNQVKIFDTTLRDGEQSPGISLDVAEKLEIAEQLARLGVDIIE
                     AGFPIASPGDFESVEAIARSVRGPVITALSRTGFQDIDRAWAAVQHAEQPRIHVFIAT
                     SLIHMKKKLRMTEDQVKAEVVAGVSRARSYTDDVEFSPEDGSRSDVDFMCDVLQLAVD
                     NGATTLNIPDTVGYGVPEEFGALIAHVIDRVDGDYVVSTHCHNDLGLAVANSLAGVQA
                     GARQVECAVNGLGERAGNAALEEIVMAIKTRRDYFGGVETGVRTEELARTSRLVSRLT
                     GYPVQYNKAVVGRNAFAHEAGIHQHGVLEDRETYEIIDASTVGQEAAQIVLGKHSGRH
                     AFSDTLEKMGLRLQGDALNQAFRRFKELADRKVQLTDVDLEAIVAEELGTGAVHRFAI
                     ASLDVHGGTQSTPVAKVVLTDGDAKVDAEAEGNGMVDAVIAAMAKATGVSGTVLDFKV
                     SSVTGGGDALGDVVIQLQADGHTASGRGLATDIVEASARAYLNAVNKLVRLRDRAPRE
                     RTVGP"
     gene            2188..>2600
                     /locus_tag="VIJ44_04890"
     CDS             2188..>2600
                     /locus_tag="VIJ44_04890"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY5165268.1"
                     /translation="MTVNLWTEAAHARDFLERRHRIPHLDEGYTALLEFLPPTPHRVL
                     DLGTGDGYLLALVRDAHPHVTGVAADFSAEMLDRARTRFADVPVEVVEHDLDQPLPTS
                     WGQFDAVVSSAAIHHVTDDRKQALYRDVFDRLVPGG"
BASE COUNT          414 a          954 c          854 g          378 t
ORIGIN      
        1 ccggtccccc gcgacgtcgt ggaccgcctc cgcgacgaac cggggatcct cgacgcccgg
       61 gccctcgagc tcgactgagc ccgattcagc gcttcgcggg tcgcaacccg tcggcctccg
      121 cggcgccccc atcccggtag cagcggtccg ggcgggtgcg ctcgtagctc tgcccgcccg
      181 gaaggtggta gatgccgctg gccagcttgg ccttcaccgg atgggacgcg gggcacaccc
      241 cattgtcggg ctccacccac gtcgacgtcg tgcccggcga cgggtccgcg gctcgcggct
      301 ggggtgggaa cggctgcggg tcccaggcca cttcggtggc acggctcgcc tcgatcgccc
      361 gccacaccgc gtacccggtg cccgccagga ccccgagccc gaacgcccgc cacacggtgc
      421 tgcgccgcat cgctgtcgat tgtcgcacgc cgcgcgccaa ctgacttgta attcgccgcg
      481 atcacggcca tcatggatag cgatgtccga gcacgccgac cgaatcatcg atcaccagag
      541 cctgctcctt acgtagcggc gagcacggtt gtgctcgcgg acgcctcctc gtgccttcgg
      601 gccggggagg tttttcgttg gaatgagcaa gcccagacgg gagccagtga tgacgaacca
      661 ggtgaagatc ttcgacacca ccctgcgtga cggcgagcag tcgcccggaa tctcgctcga
      721 cgtggccgag aagctcgaga tcgccgagca gctggcccgc ctcggtgtcg acatcatcga
      781 ggccggcttc ccgatcgcca gcccggggga cttcgagtcc gtcgaggcga tcgcgcgcag
      841 tgtgcgggga ccggtcatca ccgccctgtc ccgcaccggc ttccaggaca tcgatcgggc
      901 ctgggcggcg gtgcagcacg ccgagcagcc ccgcatccat gtgttcatcg cgacgtcgct
      961 gatccacatg aagaagaagc tgcggatgac cgaggaccag gtgaaagccg aagtcgtcgc
     1021 tggcgtcagt cgggcccgct cgtacaccga cgacgtcgag ttctcccccg aggatggcag
     1081 ccgctccgat gtcgacttca tgtgtgacgt gctccagctc gccgtcgaca acggcgccac
     1141 caccctgaac atccccgaca ccgtcgggta cggcgtcccc gaggagttcg gagcgttgat
     1201 cgcgcacgtc atcgaccggg tcgacggtga ctacgtcgtc tccacccact gtcacaacga
     1261 cctgggcctg gcggtggcga actcgctcgc cggggtccaa gccggcgccc gccaggtcga
     1321 atgcgcggtg aacggcctgg gggagcgggc cggcaacgcc gccctggagg agatcgtcat
     1381 ggcgatcaag acccgccgcg actacttcgg tggcgtcgag accggggtcc ggaccgaaga
     1441 gctggcccgc acctcgcggc tggtgtcgcg tctcaccggc tacccggtgc agtacaacaa
     1501 ggccgtcgtg ggccgcaacg cgttcgcgca cgaggcgggc atccatcagc acggtgtgct
     1561 cgaggaccgc gagacctacg agatcattga cgcctccacc gtcggccaag aggccgccca
     1621 gatcgtcctc ggcaagcact cgggtcgcca cgcgttctcg gacacgctcg agaagatggg
     1681 gctgcgcctc cagggcgatg ccctgaacca ggcgttccgg cgattcaagg agctcgccga
     1741 ccgcaaggtg cagctgaccg acgtcgacct cgaggcgatt gtcgccgagg agctcgggac
     1801 cggcgcggtg caccggttcg ccatcgccag cctcgacgtg cacggcggca cccagtccac
     1861 cccggtggcc aaggtcgtcc tgaccgacgg tgacgccaag gtcgacgccg aggcggaagg
     1921 caacgggatg gtcgacgcgg tcatcgccgc gatggccaag gcgacggggg tcagcgggac
     1981 cgtcctggac ttcaaggtgt cgtcggtgac cggaggtggc gacgcgctcg gcgacgtcgt
     2041 catccagctc caagccgacg gccacaccgc gtcggggcga ggactcgcca ccgacatcgt
     2101 cgaggcgtca gcccgcgcgt atctgaacgc ggtcaacaag ctggtgcggc tgcgcgaccg
     2161 cgccccccgg gaacggaccg tcgggccatg accgtcaacc tctggaccga ggccgcccac
     2221 gcccgcgact tcctcgaacg gcgccaccgc atcccgcacc tcgacgaggg atacaccgcc
     2281 ctgctcgagt tcctcccccc gactccccat cgggtcctgg acctcggaac cggcgacggc
     2341 tacctcctcg cactggtccg cgacgcccat ccacacgtca ctggcgtcgc ggccgacttc
     2401 tccgccgaga tgctcgaccg agcccggacc cgtttcgccg atgtcccggt cgaggtcgtc
     2461 gagcacgacc tcgaccagcc gctcccgacc tcatggggcc agttcgacgc ggttgtctcc
     2521 tcggccgcca tccaccacgt caccgacgac cgcaagcagg ccctctaccg cgacgtgttc
     2581 gaccggctgg tccccggggg
//
LOCUS       DATCCH010000249         3800 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_110516, whole genome shotgun sequence.
ACCESSION   DATCCH010000249 DATCCH010000000
VERSION     DATCCH010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110516"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VIJ44_04895"
     CDS             complement(<1..252)
                     /locus_tag="VIJ44_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165269.1"
                     /translation="MADSKSTAIHIRSIDLGWRARTRPASGFAHVLAAGSGAFAVFAV
                     TELVTKITSTDATAPGILLNLALAVGAFVAGMFLPGPVRS"
     gene            complement(407..895)
                     /locus_tag="VIJ44_04900"
     CDS             complement(407..895)
                     /locus_tag="VIJ44_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165270.1"
                     /translation="MDLSKLTRGDQVIGISGIVLFIFSFFSWLGATVSVGARGLAGFS
                     ASGSDNAWGFTLTLLAVLIGIALVAYVVLKAAGVEIPDKFGSVTLAQILLGLAAVAFL
                     FVLIKIIAGPNLPSVPTGISVSKDRKFGIFIGLLATAGLVAGAFLNFQESQSGSGSPP
                     AA"
     gene            1114..1677
                     /gene="purN"
                     /locus_tag="VIJ44_04905"
     CDS             1114..1677
                     /gene="purN"
                     /locus_tag="VIJ44_04905"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010119609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEY5165271.1"
                     /translation="MRRLGVLASGTGTILQALLDAGLPVGVVVVDRPCGATGRAEAAG
                     VPVELVERTSFGRSFDRGAYTHRVVDALKRHEVDLVAMAGFGTILAEPIHDAFGGRVV
                     NTHPALLPAFRGWHAVEDALGAGVKVTGWTAHLAILEVDDGPILAQEAVPVLEGDTVE
                     TLHERIKEVERRRYPEVLRDLLDEESS"
     gene            1674..3182
                     /gene="purH"
                     /locus_tag="VIJ44_04910"
     CDS             1674..3182
                     /gene="purH"
                     /locus_tag="VIJ44_04910"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005380229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEY5165272.1"
                     /translation="MRRRALVSVYDKAGLVDFARGLRELGYELVSSGGTAAALADAAV
                     EVTTVESVTEAPEMLDGRVKTLHPKVHGGLLADLGNEQHVADLERQSIEPFQLVVSNL
                     YPFPTNPGVEMIDIGGPAMVRAAAKNHAWVAVVSSPTQYDDVLAELRAEGGTLTGETR
                     RRLAREAFARTAAYDAAIVAWLEGDDGLPEHLVLALERTADTLRYGENPHQRAARYRV
                     SGTTSWWDEVRQHSGLALSYLNFYDADAAWRIVHDLGDDAACAIIKHANPCGAAVSRD
                     LRDAYQRALECDEQSAFGGVVAFNRPVDAATAQRMVSGPQADVVIAPGYGDGTVDALI
                     GKRRNTRLLEGPPPVAWRRDVRQISGGFLIQDPHHFVAQRDAWRVVTKAAPTEAQWRD
                     AELAWRLCGHVKSNAIVLVKDGQAVGIGAGQQKRVDSGEIAARKAAGRAAGGACASDA
                     FYPFPDGIDAAAGAGVAVVVQPGGGVRDDQIIERADELGLAMVFTAERHFLH"
     gene            3182..>3800
                     /locus_tag="VIJ44_04915"
     CDS             3182..>3800
                     /locus_tag="VIJ44_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HEY5165273.1"
                     /translation="MAALMMPGAPVADAVFADLEPRIAALVEAGHRPGLGTILVGEES
                     ASVGYVRIKHQKAAEIGCASPHIQLADDASQADLVAAVRSFNDDPTVDAMLVQHPTPP
                     QIDYEAALLEIDPDKDADGLHPVNMGRLAMSMAGPVPCTPAGIEALLAHYQVPVAGHE
                     VCILGRGMTLGRPLAMLLSQKRPTANAAVTVVHTGVPDWARYTRRA"
BASE COUNT          621 a         1271 c         1329 g          579 t
ORIGIN      
        1 gctccgcacc ggcccgggca ggaacatccc ggcgacgaat gcgccgacgg cgagggccag
       61 gttgagcaag atgccgggcg ccgtcgcgtc cgtgctggtg atctttgtca ccagctctgt
      121 gacggcgaac accgcgaacg cgcccgaacc agcggcgagg acgtgcgcga accccgacgc
      181 cggccgtgtc cgggcccgcc agccgagatc aatcgaacgg atgtgaatcg ccgtcgactt
      241 ggaatcagcc atgaccccaa ccctccggtg tgtagacaga agagtctggc gaacaaatgt
      301 caggcgcgca aagacgatcc ggggccggtg caccccaggt cggaggggcc ggaaatgaca
      361 aggcccccgc catggcgggg gccttgtcta gacactggtg tcgcggtcag gcggctggcg
      421 gtgagcccga cccactctgg gattcctgga agttcaggaa ggcgccagcg acgagtccag
      481 cggtcgcgag gagcccgatg aagatcccga acttgcggtc cttgctgacg ctgatcccgg
      541 tcgggacgct ggggaggttc ggtcccgcga tgatcttgat caggacgaac aggaacgcga
      601 cggccgcgag gccgagcagg atttgcgcca acgtgaccga gccgaacttg tccgggatct
      661 cgaccccggc ggccttgagg acgacgtacg ccaccaacgc gatgccgatc agcaccgcga
      721 ggagcgtcag ggtgaaaccc caggcgttgt cggagccgga ggcgctgaag cccgcgagtc
      781 ctcgggcgcc cacgctcacg gtcgcgccga gccaactgaa gaatgagaag atgaatagga
      841 cgattccgct gatgccgatg acttggtcgc ctcgggtcag cttgctgaga tccatgcagg
      901 ttcctccgaa gaacgcgtcg acgccaggct gccggcgcca accagcagtc gatcacggcg
      961 ccccccggaa tgcaagcatc ggcactgaca ccaacgccgc catcgacatc cttcgggccg
     1021 gtagggccca gcagcaaaac gctgcgcagg gtcgaaactg acccactacc caagcatcgt
     1081 cgatttggtg ggggctcggc taccttcctt gctgtgcgga ggttgggcgt gctcgcgtcg
     1141 gggaccggca ccatcctcca ggccctcctc gacgcaggtc tgccggtggg ggtggtcgtc
     1201 gtcgaccggc cgtgtggtgc cacgggccgt gctgaggccg cgggagtccc cgttgagctc
     1261 gtcgagcgca cgtcgttcgg ccgtagcttc gaccggggtg cctacacgca ccgtgtcgtc
     1321 gacgccctca aacgtcacga ggttgacctc gtggcgatgg ccggtttcgg gacgatcctt
     1381 gccgagccga tccatgacgc cttcggaggc cgggtcgtga atacccatcc ggcgctcctc
     1441 ccggcgttcc gcggctggca cgcggtggag gatgcgctcg gtgctggggt gaaggtgact
     1501 gggtggacgg cgcacctcgc gatcctcgag gtggatgacg gtccgatcct cgctcaggag
     1561 gccgtgccgg tcctggaagg cgacaccgtc gagacgttgc atgagcgcat caaggaggtc
     1621 gaacggcgcc ggtatcccga ggtgctccgc gatctgctcg acgaggagag ctcgtgaggc
     1681 gacgggccct cgtgtcggtg tacgacaagg cgggcctggt cgacttcgct cgtggcttgc
     1741 gcgagctggg ctacgagctc gtgtcgtcgg gtgggaccgc cgccgcgctc gccgatgccg
     1801 ccgtggaggt cacgacagtc gagtcggtga ccgaagctcc cgagatgctc gacggtcggg
     1861 tcaagacgtt gcatccgaag gttcacggtg gactcctcgc ggacctgggc aacgagcagc
     1921 acgtcgcgga cctggaacgc cagtcgatcg agccctttca gctcgtggtc agcaacctgt
     1981 acccgttccc gacgaatccc ggtgtggaga tgatcgacat cgggggcccg gcgatggtgc
     2041 gggccgcggc gaagaatcac gcttgggtcg ctgtggtgtc cagtcccacc cagtacgacg
     2101 acgtactggc ggagctccgg gccgaaggcg gcaccctcac cggcgagacc cgccgtcgtc
     2161 tcgcccgtga ggccttcgcg cgaacggcgg cctacgacgc ggcgatcgtc gcgtggctcg
     2221 aaggcgacga cggcctcccc gagcatcttg tcctcgccct cgagcgcacc gcggacacgc
     2281 tgcgttacgg cgagaacccc caccagcgcg ccgcccgtta ccgggtttcg ggtacgacgt
     2341 cgtggtggga cgaggtacgt cagcacagcg ggctggcgct ctcgtacctg aacttctacg
     2401 acgccgacgc cgcatggcgg atcgtgcacg acctcgggga cgacgcggcg tgcgccatca
     2461 tcaagcacgc caacccgtgc ggtgccgcgg tgtctcgtga tcttcgtgac gcctaccagc
     2521 gggcgctcga gtgcgacgag cagtcggcgt tcggtggtgt cgtggcattc aaccgaccag
     2581 tggatgcggc gaccgcgcaa cgcatggtga gcggtcccca ggccgacgtc gtcatcgcac
     2641 ccggctacgg cgacgggacc gtcgacgccc tcatcgggaa acgccggaac actcgactgc
     2701 tcgaagggcc acccccagtg gcgtggcgcc gcgacgtccg gcagatctcg ggtggtttct
     2761 tgatccagga cccgcaccat ttcgtggccc agcgtgatgc gtggcgcgtc gtcaccaagg
     2821 ccgccccgac cgaggctcag tggcgcgacg ccgagctggc ctggcgactg tgcgggcatg
     2881 tgaagtcgaa cgcgatcgtc ctggtcaagg acggccaagc tgtcgggatc ggcgccggtc
     2941 agcagaaacg tgtcgattcc ggtgagatcg cggcccgcaa ggcggccggc cgtgccgccg
     3001 gaggcgcctg cgccagcgac gcgttctacc ccttcccgga tggcatcgac gccgccgccg
     3061 gcgcgggcgt cgcggtcgtc gtgcaaccgg gtggcggtgt gcgcgacgac cagatcatcg
     3121 aacgcgccga cgagctcggt ctggccatgg tgttcaccgc cgaacgccac ttcctgcact
     3181 gatggccgcg ctgatgatgc cgggtgcccc ggtcgcggac gcggtgttcg ccgaccttga
     3241 gccccggatt gccgcgctgg tcgaggcggg gcatcgaccg ggcctgggga cgatcctcgt
     3301 cggcgaggag tcggccagcg tcgggtacgt ccggatcaag caccaaaagg ccgcggagat
     3361 cgggtgcgcg tcgccgcaca tccaactggc cgacgatgcc agccaagcgg accttgtcgc
     3421 ggcggtccgc tcgttcaacg acgacccaac ggtcgacgcc atgctcgtgc agcacccgac
     3481 gccgccgcag atcgactacg aggcggcgct gctcgagatc gatcccgaca aagacgctga
     3541 cggcctgcat ccggtcaaca tggggcgact ggcgatgtcg atggcgggtc cggtcccgtg
     3601 cacacccgcc ggcatcgagg cactcctcgc ccactaccag gtgccggtgg ccgggcacga
     3661 ggtctgcatc ctcggtcgcg ggatgaccct cggccggccg ctggcgatgc tgctctccca
     3721 gaagcgcccg acggcgaacg ccgcggtcac ggtcgtgcac accggggttc cagattgggc
     3781 ccgctacacc cgccgggccg
//
LOCUS       DATCCH010000250         2117 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_193652, whole genome shotgun sequence.
ACCESSION   DATCCH010000250 DATCCH010000000
VERSION     DATCCH010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193652"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..1209)
                     /locus_tag="VIJ44_04920"
     CDS             complement(100..1209)
                     /locus_tag="VIJ44_04920"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEY5165274.1"
                     /translation="MSDPAAPGRVLGGRYRLVSALARGGMATVWVSQDTLLDRRVAVK
                     TLHPELSGDAGLRNRFRHEAVAAARLSSPGIVATYDTGEDGGVGYIVMEFVDGPNLRQ
                     VLDDRGPLPIADTARIGREVATALDHAHRQGVIHRDVKPANVLVPPGGSVKVTDFGIA
                     KAGGGELTRTGVVVGTARYLAPEQLRGEPVDARTDIYALGLVLYEMLTGELPFHGDTE
                     MAISLARLSVAPVPVEIGRPDTPAGLARLVMACLAVEPADRPPSAQAVADALTGRPRP
                     GPTPVAPRAERTRTTVTAPRDAPPRSRRAPAPRPRPTPPRRGPVAVLAVLLFVAGIAA
                     GYLMVHAAVAGASPPGPTPTAPAPAPPTPATPAGG"
     gene            complement(1259..>2117)
                     /locus_tag="VIJ44_04925"
     CDS             complement(1259..>2117)
                     /locus_tag="VIJ44_04925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEY5165275.1"
                     /translation="IGTRRPPVPVRIVTDSACDLPDAVVAELGIEIVPLTIRFGSEEL
                     VDRVELTNQAFWQRLATAPTLPETAAPSAGAFAARFRALSDAGADGIVCINLSSRLSA
                     TMQAAQVAAPSVAEHCRVEVIDSLTASMGLGNLCIAAAKRAAAGAPMDEIVTEVTHRR
                     DRTRLFGTLDTLEFLKKGGRIGNARALLGSMLAVKPVVEIRDGVVEEAGKVRTRSKAL
                     RLLAEKVHEGSVEDVAVLHGDARDIDEFLDLIDDVAPRDKIVVGAVGPVIGTHAGPGV
                     LGVTYQLAD"
BASE COUNT          283 a          727 c          823 g          284 t
ORIGIN      
        1 tcgctcgagg agccgctcca gggcgtcccg gtcaccgccg gcggctgcgg cggcgaggaa
       61 cccgtcggcc gggcccgtct cctcggcggg tggcgtgact cagcccccgg cgggggttgc
      121 cggggtcggc ggtgccggtg ccggtgccgt gggcgtcggt cccggcgggc tcgccccggc
      181 cacggcggcg tgcaccatca ggtagccggc tgcgatcccg gcgacgaaca ggagcaccgc
      241 gagtaccgcc accgggcccc gccgcggcgg tgtcggccgg ggtcgggggg cgggcgcacg
      301 acggctgcgg ggcggagcgt cccgaggcgc agtgacggtg gtgcgggttc gttcggcgcg
      361 cggcgcgacc ggggtcgggc cgggtcgggg ccgtccggtg agggcgtcgg cgacggcctg
      421 ggcgctgggg ggtcgatcgg cgggctcgac cgcgaggcaa gccatgacga gccgggccag
      481 gccggcaggg gtgtcgggtc gaccgatctc gacggggacc ggggccacgc tcagccgagc
      541 cagggagatg gccatctcgg tgtcgccgtg gaaggggagt tcgccggtga gcatctcgta
      601 gaggaccagg ccgagggcgt agatgtcagt gcgggcgtcg accggctcgc cgcggagctg
      661 ctcgggtgcg aggtagcggg cggtgccgac cacgacgccg gtgcgggtga gttcgccgcc
      721 gccggccttg gcgatgccga agtcggtgac cttgacggac ccgccgggcg ggacgaggac
      781 gttggctggt ttcacatccc ggtggatgac gccctgacgg tgggcgtggt cgagcgcggt
      841 ggcgacctcc cggccgatcc gggcggtgtc ggcgatcggc agcgggccgc ggtcgtcgag
      901 gacttgccgc aggttcggac cgtctacgaa ctccatgacg atgtagccga cgccgccgtc
      961 ctctccggtg tcgtaggtgg cgacgattcc cgggctgctg agccgggccg cggcgacggc
     1021 ctcgtgtcgg aaccggtttc gcagtccggc atcccccgac agctccgggt gcagcgtctt
     1081 gacggcgacg cgccggtcca gcagcgtgtc ctggctgacc cagacggtgg ccatcccacc
     1141 gcgggcgagg gcgctcacga gccggtaccg gccgccgagc acacgaccgg gcgcggcagg
     1201 atcagacacc gcactcacgc tacccagcgg ggcagcgcga agccgccgcg gacgaggctc
     1261 agtccgcgag ctgataggtg acgccgagca ccccgggacc ggcgtgggtc ccgatcacgg
     1321 gcccgaccgc ccccaccacg atcttgtcgc ggggagcgac gtcgtcaatc aggtcaagga
     1381 actcatcgat gtcgcgggcg tcgccgtgga gcaccgcgac gtcttcgacc gaaccttcgt
     1441 ggaccttctc ggcgaggagc cgcagcgcct tcgaacgggt ccggaccttg ccggcctcct
     1501 cgacgacgcc gtcgcggatc tcgacgacgg gcttcacggc cagcatcgag cccagcagcg
     1561 cccgggcgtt gccgatgcga ccacccttct tcaagaactc caaggtgtcg agcgtcccga
     1621 agagacgggt ccggtcgcgg cggtgggtga cctcggtgac gatctcatcc atcggcgcgc
     1681 ccgcggcggc ccgcttcgcc gccgcgatgc agaggttccc gagacccatc gaggcggtga
     1741 gcgaatcgat cacctcgacc cggcagtgct cggcgacgga cggtgccgcg acctgcgccg
     1801 cttgcatcgt ggccgacaga cgcgacgaca ggttgatgca gacgatcccg tcggcgccgg
     1861 cgtcgctcaa cgcccggaac cgagcggcga acgcgcccgc cgagggcgct gcggtctcgg
     1921 ggagcgtcgg cgcggtcgcg agccgctgcc agaacgcctg gttcgtcagc tccacgcggt
     1981 cgacgagctc ctcggatccg aaccggatcg tgaggggaac gatctcgatc cccaactccg
     2041 cgacgaccgc gtccggtaga tcgcaggcgc tgtcggtgac gattcggact ggcacgggtg
     2101 gcctcctggt cccgatg
//
LOCUS       DATCCH010000251         2748 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_345859, whole genome shotgun sequence.
ACCESSION   DATCCH010000251 DATCCH010000000
VERSION     DATCCH010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345859"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1208)
                     /locus_tag="VIJ44_04930"
     CDS             complement(<1..1208)
                     /locus_tag="VIJ44_04930"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY5165276.1"
                     /translation="MARSAVADQVGRVLVDRYRLLAPIGVGASGRVYLADDVRLRRRV
                     AVKVLQPGLADDAGFVRRFRAEAQLAASLHHPNVMAVYDWGQDDGVAFMVVELLRGGS
                     LRALLDTGTLLTPAQSLQVGGQVAAALQYAHARGLVHRDIKPANLLFDEHGVVRVGDF
                     GLARALAEAAWTEPVGALVGTARYAAPEQGGAVPPDARADLYSLAVVLVEACTGEVPV
                     VADTPIGTLAARTVRGIVAPPELGALGAVIERAGRPQRTERYPDAATMGAALSDAARR
                     FPPPDPLPLAGLGEEALDADPTRLGATALTSPGPRRETDGTVDDAARLAVPVHAPPGR
                     RTSIVPYAVGAAIVAAIVVVAALLLSGGSSGSVAAPTLIGRPEPTAATDATNTGVLLK
                     VVERRRADDPA"
     gene            1298..1777
                     /locus_tag="VIJ44_04935"
     CDS             1298..1777
                     /locus_tag="VIJ44_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165277.1"
                     /translation="MSSTSNPPALVTGAERARPPLFKHPARIAIVVLGLIAVVNLGVF
                     VLARSDTSRESTTGLPIDIVSLQPRPGELIRPQDTVTAQVRTSLTGVLVLDGQEIPED
                     QTDRVPELGEISFRPGAGKDLRQFPPGTHTLVVKYWLQGKARPAHPAAFAWTFRVGA"
     gene            complement(1781..>2748)
                     /locus_tag="VIJ44_04940"
     CDS             complement(1781..>2748)
                     /locus_tag="VIJ44_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEY5165278.1"
                     /translation="AEQRPLALEDRPAPVPAAGEARVRVHACGCCRTDLHVVEGDLEL
                     PMLPIVPGHQVVGTVDALGADCTRLSMGDRVGVACLHETCGVCPFCRRGEENLCERAR
                     FTGWTADGGYADWLTVPEGFAVHVPDELTDLEAAPLLCAGVIGYRALRRAEVEPGERV
                     GLMGFGSSAHLVLQLLAYWGCDTVVLTRGARHRELARELGASWVGDAAELPPVPCDRV
                     VVFAPAGELVPVALQLVRPGGVVSLAGIHMSPIPTFDYSLLWRERTLRSVANMTRRDA
                     DEFMALAALATIRAEVECYSLEDAPEALIAIAEDQVRGSAVLAVS"
BASE COUNT          425 a         1058 c          920 g          345 t
ORIGIN      
        1 gccggatcgt cggcccgtcg gcgctccacc accttcagga gcacgccggt gttggtcgcg
       61 tcggtcgccg ccgtcggctc gggtcgaccg atcagggtcg gtgccgccac cgacccggac
      121 gaacccccgc tgagcaacaa cgcggcgacg accacgatcg ccgccacgat ggccgccccc
      181 accgcgtacg gaacgatgct ggtgcgccgc cccggcgggg cgtgcacggg aacggcgaga
      241 cgtgccgcgt catcgaccgt gccgtccgtc tcgcggcggg ggcccgggct ggtaagggcg
      301 gtcgcaccga ggcgcgtcgg atctgcgtcg agggcttcct cgccgaggcc ggcgagcggc
      361 agcggatccg ggggcgggaa gcgacgtgcc gcgtcggaca acgccgcgcc catggtcgcc
      421 gcgtcggggt accgctccgt gcgctggggc cggcccgccc gctcgatgac cgcgccgagc
      481 gcgccgagct cgggcggggc gacgatcccc cggaccgtgc gtgccgcgag cgtcccgatc
      541 ggtgtgtcgg cgaccacggg cacctctccc gtgcacgcct cgaccaacac gacggccagc
      601 gaatacaggt cggcccgggc gtcgggcggc accgcaccgc cctgctcggg cgccgcgtag
      661 cgggcggtgc ccaccagggc acccaccggt tcggtccagg cagcctcggc gagcgcccgc
      721 gccaacccga agtcgccgac gcggacgacg ccatgctcgt cgaagagcag gttcgccggc
      781 ttgatgtctc gatgaacgag ccctcgggcg tgcgcgtact ggagcgccgc ggccacctgt
      841 cccccgacct gcaggctctg cgccggggtg aggagcgtgc ccgtgtcgag cagcgcccgc
      901 agcgaccctc ctcgcaacag ctcgacgacc atgaacgcca cgccgtcgtc ttggccccag
      961 tcgtataccg ccatgacgtt cgggtggtgc agcgacgccg cgagctgcgc ttcggcacgg
     1021 aaccgccgga cgaacccggc gtcgtcggcg agtcccggtt ggagcacctt gaccgcgacc
     1081 cgacggcgga gccgcacgtc gtcagcgagg tagacccggc cactcgcgcc cacgccgatc
     1141 ggagccagca gccggtatcg gtccacgagg acgcgcccaa cctgatcggc gacggcgcta
     1201 cgagccacca cgccaggcta cgagccacct cggcggatct tgcggcatcc ggccgcgatg
     1261 ctttcgccct atgacggcgc cggtaccctc gcccgtggtg tcgagcacgt cgaacccacc
     1321 cgcattggtc accggtgccg aacgcgcgcg accgccgctg ttcaaacacc cagctcggat
     1381 cgcgatcgtg gtgctcgggt tgattgccgt ggtgaacctc ggcgtcttcg tcctcgcgag
     1441 atccgacacc tcccgggaga gcaccaccgg gctcccgatc gacattgtga gtctgcagcc
     1501 gagaccaggc gagctcatcc ggccccaaga cacggtgacc gcgcaggtcc gcaccagcct
     1561 cactggcgtc ctcgtcctgg atggccaaga gatcccggaa gaccagaccg accgcgtccc
     1621 cgaactgggc gagatcagct tccggcccgg cgccggcaag gatctgcgcc agttcccgcc
     1681 gggaacccac accctcgtcg tgaagtactg gctccagggc aaggcccgac cggcgcatcc
     1741 cgccgcgttc gcctggacct tccgggtcgg tgcctgaccg ttacgacacc gccaggaccg
     1801 ccgagcctcg gacctggtct tcggcgatcg cgatcagcgc ctcgggcgcg tcctcgagcg
     1861 aatagcactc gacctcggcc cggatggtgg ccagcgccgc gagcgccatg aactcgtccg
     1921 cgtcgcgcct cgtcatgttc gccaccgacc gcaacgtccg ttcgcgccac aacaacgagt
     1981 agtcgaacgt cgggatcggt gacatgtgga ttcccgccaa gctgaccaca ccgcccggcc
     2041 ggacgagctg caacgcgacc ggcaccagct cgcccgcagg ggcgaacacc acgacccggt
     2101 cgcacggaac cggcggcagt tccgcggcgt caccaaccca cgaggcaccc agctcgcgcg
     2161 ccagctcccg gtggcgtgcg ccgcgggtca ggaccacggt gtcgcacccc caataggcga
     2221 ggagctgcaa gacgagatgc gccgaagagc cgaagcccat gagcccgacc cgctcgccgg
     2281 gctcgacctc ggcgcgccgg agcgcccggt acccgatcac gcccgcgcag agcagcggtg
     2341 ccgcttcgag gtccgtgagc tcgtccggga cgtggaccgc gaacccctcc gggaccgtca
     2401 gccagtcggc gtaccctccg tcggcggtcc aaccggtgaa gcgtgcccgt tcgcagagat
     2461 tctcctcgcc gcgacggcag aacggacaca caccgcacgt ctcgtgcaga cacgcgacgc
     2521 ccactcgatc tcccatgctc agccgcgtgc agtccgctcc gagcgcgtcg acagtgccga
     2581 caacctgatg cccggggacg atcggcagca tcggcagctc gagatcgccc tccacgacgt
     2641 gcaggtcggt ccgacagcat ccgcacgcgt gcacccgaac gcgcgcttcg ccggccgcag
     2701 ggacgggagc cggtcggtcc tccagcgcga gcggacgctg ctccgccc
//
LOCUS       DATCCH010000252         2431 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_317218, whole genome shotgun sequence.
ACCESSION   DATCCH010000252 DATCCH010000000
VERSION     DATCCH010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317218"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /locus_tag="VIJ44_04945"
     CDS             <1..859
                     /locus_tag="VIJ44_04945"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY5165279.1"
                     /translation="RPHPPVWVACSRRDTIHLAAQHGIGALTFAFIDPEEAQHWIGDY
                     EQTLAEECVPIGDAVNANVACVSPFMCHRDEDEALARGLEGGNFFGYSLAHYYLFGRH
                     EPGRTDVWAEYQARRAEHGYDPDAVAAANTNGERLGAKVVQAGSFGIRGAIGTPDQLR
                     DYLRRYEECGVDQLIFCAQVGKNRHEDIMESLELFGADVLPEFRDRDEAQRRAKARRL
                     EPIVDAALARKPASDHPPLPSPGYAYPAIPRALADRSGSDDFHRWLDELAEKTATGEQ
                     PEEFRQLLT"
     gene            1004..2290
                     /locus_tag="VIJ44_04950"
     CDS             1004..2290
                     /locus_tag="VIJ44_04950"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HEY5165280.1"
                     /translation="MSAISDRERIAHVVRRLSMGSHPDLVGQLSDPDTAIARALDLAG
                     APAVPPAMDPPPSLQASQPAQIATLIDWWVDQMQSSSRLVHERLTWFWHDHFATSLLK
                     VRAPYLMRQQHVTIRAHASGSFADLLHAVAKDPAMLLFLDGFTNTAGKVNENFGRECL
                     ELFTVGRDSGYTQDDVVAASRSFTGWVVNLAGTPTSSRLASVGIAPWASGLVPNRHDR
                     LVKTLLGRDGNFDLDGALDVILDQPATGTFIATKLYRTLIGREPDATTARRLGAAFAH
                     DYQIMGLVDAIVRDPAFTADTAVRTRVRTPVEKLVGIHQAAATGHGGTTTTPRRPAAV
                     ANVLHAADYLPFLPPNVGGFPQGSLLLGPHDLVSTFALLATLDVPPSPPKSVDALLAR
                     FSLFDVSSTTRHVLEAERDPGRRFALAAMSPEFAVT"
BASE COUNT          358 a          911 c          776 g          386 t
ORIGIN      
        1 acgtccgcat ccaccggtgt gggtggcgtg cagccgacgt gacacgatcc acctcgcggc
       61 tcagcacggg atcggtgcgc tcaccttcgc gttcatcgac cctgaagagg cccaacattg
      121 gatcggggac tacgagcaga cgctggccga ggagtgtgta ccgatcggcg atgcggtcaa
      181 cgccaacgtc gcgtgtgtca gccccttcat gtgtcaccgc gacgaggacg aggcgctggc
      241 tcgaggcctc gagggcggca acttcttcgg atactcactg gcccactact acctgttcgg
      301 tcgtcatgag cccggccgca ccgatgtgtg ggccgagtac caggctcgtc gagctgagca
      361 cggctacgac cccgacgccg tcgccgcggc aaataccaat ggtgaacggc tcggcgccaa
      421 ggtcgtgcaa gcagggtcat tcgggatccg gggggcgatc ggcacccccg accagttgcg
      481 cgactacctg cggcgatacg aggagtgcgg cgtcgaccag ctgatcttct gcgcccaggt
      541 cggaaagaac cgtcatgagg acatcatgga gagcctcgag ctcttcgggg ccgacgtcct
      601 ccccgagttc cgcgaccgcg acgaggccca gcggcgagcc aaagcccgac gcctcgagcc
      661 gatcgtcgac gccgcgttgg ctcgcaagcc cgcgtcggat catccgccgc tcccgtcccc
      721 tggctacgcg taccccgcca tcccgcgggc gctcgcggac cgcagcggca gcgacgactt
      781 ccatcggtgg ctcgacgagc tcgccgagaa gaccgccacc ggcgaacagc ccgaagagtt
      841 ccgccagctg ctgacctgaa ctgcgtcccg gctggtcgac ccgtcgccgg ctcgccgcat
      901 gcctcgcccg gcgcgggttg acgtgtttat tccggactgt tggttgacgg ggccctgagg
      961 cccgcttcac gtcgggatca cacgtcggcg cgacgctgtt gccatgtcgg ctatctcgga
     1021 ccgcgagcgc atcgcccacg tcgtgcggcg cctctcgatg ggctcgcatc ccgatctggt
     1081 gggccagctc tccgacccgg acacggccat cgctcgagcc ctcgatctcg ccggcgcccc
     1141 agcggtgccg ccggccatgg acccgccgcc gtcgctccag gcctcgcagc cagcccagat
     1201 cgccaccctg atcgactggt gggtcgacca gatgcagtcg agctcgcggc tggtccacga
     1261 acgcctgacc tggttctggc acgaccactt cgcgacgagc ctcctgaagg tccgcgcccc
     1321 gtatctgatg cgccagcagc acgtgaccat ccgggcccac gcctctggga gcttcgccga
     1381 tcttctccac gccgtcgcga aggacccggc gatgctgctc ttcctggacg gattcacgaa
     1441 caccgccggg aaggtcaacg agaacttcgg gcgcgagtgc ctcgagctgt tcaccgtcgg
     1501 ccgcgactcg gggtacaccc aagacgacgt cgtcgccgcg tcgcggtctt tcaccgggtg
     1561 ggtggtcaac ctggccggga ccccgacgtc gtctcgcctc gcttcggttg ggatcgcccc
     1621 ctgggcctcg gggctcgtcc cgaaccgtca tgaccgcctg gtcaagacgc tgctgggccg
     1681 cgacgggaat ttcgatctcg acggcgcgct tgacgtgatc ctcgaccagc cggccaccgg
     1741 gaccttcatc gccaccaagc tgtaccggac gctgatcggc cgggaaccgg acgcgacgac
     1801 ggcgcgtcga ctcggcgccg cgttcgccca cgactaccag atcatggggc tcgtcgacgc
     1861 catcgtccga gatccggcct tcaccgctga caccgcggtg cgaacccggg tgcgcacccc
     1921 ggtcgagaag ctcgtcggca tccaccaagc cgccgcgacc ggccacggtg gcactaccac
     1981 cacgccgcgt cggcccgcgg cggtcgcgaa cgtcctccac gcggccgact acctcccgtt
     2041 tctcccaccc aacgtcggcg gcttccccca agggtcgctg ctcctcggcc cccacgacct
     2101 ggtgagcacc ttcgcgctgc tcgcgacgct cgacgtgccc ccgtcgccgc cgaagagcgt
     2161 cgacgcgctg ctcgctcgct tctccctgtt cgacgtctcg tcgaccaccc gtcacgtgct
     2221 cgaagccgag cgcgaccccg gccgccgctt cgcgctggcg gccatgtcac cggagttcgc
     2281 ggtcacatga gcgacgtctc ccgccgtgac ttcctgcgtc gagtcgggct cggctcggtc
     2341 gcggtcgcct cgtcgggcta cgtgctcagc acgtgggaat gggccgggcc ggccggcgcg
     2401 gcggtcccga gacccggtga catcagctcg g
//
LOCUS       DATCCH010000253         4039 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_276730, whole genome shotgun sequence.
ACCESSION   DATCCH010000253 DATCCH010000000
VERSION     DATCCH010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276730"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VIJ44_04955"
     CDS             <1..546
                     /locus_tag="VIJ44_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165281.1"
                     /translation="PRVVFLAVALVMVPACSSSSPTAATKTNGTVGAVPARPAGKNPS
                     ASAKMVCAKEAQDALVSSLGATPVQVTTPTWVDHVYACQYVYSTGTFALSVKELDSAK
                     QTTAYYDGFAARFGRRPGRLAVGQGAYVTTDGSVVVRKDWKVLYVDVSHLPPQFGQPP
                     QAASDAALSVAATILSCWSGA"
     gene            complement(563..1255)
                     /locus_tag="VIJ44_04960"
     CDS             complement(563..1255)
                     /locus_tag="VIJ44_04960"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEY5165282.1"
                     /translation="MRRPRLRWLLLAAFLVVALGVGAVIWYVFGGSSPGKPHLSSTAT
                     TDVAPGSGTPVGSWTLLRAKDVYVGYRIQELFAGDTIHKDAVGRTPTVDGTMTITPGQ
                     VASVVVNADLQNLTSGRAARDDYIHTHAIESDTFPTAHFELASPIAIPASVRPGTKLH
                     LSAPGRLTLHGVTRDVTAALDARWNGSTIDVVGTAPIVLADYGIQAPQTAVTSVGDHG
                     TFELQLRFRHLP"
     gene            complement(1261..2811)
                     /locus_tag="VIJ44_04965"
     CDS             complement(1261..2811)
                     /locus_tag="VIJ44_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165283.1"
                     /translation="MHQRLPLLASAAAALVAVATVATPAAASPGAAAKPSNVPIVTNP
                     VPTGAQRLHFEVGPLPIRPGQNSISFSRRIPEPTADGWIVGMTTNLHLADGTVPPVDV
                     IHLHHGVWLNLSAPSLVNSRFFAAGEEKTAMALPTGYGYPFKATDKWLLSYMLHNQLS
                     KPNQVWLTYDIDFIPATAPASASMQTAQPIWMDVRNPSGYPVFDVLKGSGHNGQYTYP
                     DDATNPYGAGPARNQWTVPTDGVLLTTAGHVHPGGLFDSLWLTRKGATAPAGHAKAGS
                     ADTVRLFQSNAKYFDPAGDVSWDVAMTATPSTWRPVVHKGDVLSISTTYDSKNASWYE
                     SMGIMVVWMADGTTKGVNPFQKPVDVSGVLTHGHLAENNNHGGQPDAKHYQDVRKAPS
                     RILPSGSILPIADFAYTGDLSEAATVPAVAQGGTLTFRNDDAANGIWHTVTSCSAPCN
                     LSTGISYPLANTNVLFDSGELGHGGPPAVGTITFSTPTNLAPGTYTYFCRIHPFMRGA
                     FRVVAAKH"
     gene            complement(2877..3566)
                     /locus_tag="VIJ44_04970"
     CDS             complement(2877..3566)
                     /locus_tag="VIJ44_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016887604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEY5165284.1"
                     /translation="MDLDEALRATGAIRDFRGDPVPDEVLHRILDTARFAPSGGNRQG
                     WRVIVVNDPATRIRLRDLYLAGWYEYLAQVEAGLVPWAPITDDEAEAQAVATTPVVAE
                     RAAAGPGGFAEHLETVPVLLALLADLRALAAVDKGFDRYTFAGAASIYPFAWSILLAA
                     RGEGLGGVITTMSIRREPDVKALLGIPDELAVAAVIALGYPHHQPKRLTRGAVEQFTT
                     VDRFDGPPFGT"
     gene            complement(3620..>4039)
                     /locus_tag="VIJ44_04975"
     CDS             complement(3620..>4039)
                     /locus_tag="VIJ44_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015290119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY5165285.1"
                     /translation="HVAPPSPPPDTPDEFVTTSPLPTAAGGISGPIGLGFRVPMLVIS
                     PFSRGGHINSDTFDHTSLLRFLETRFGVEVPNLSPWRRNAVSDLTSTLQLSHPPRLGV
                     PALPYSGLSTALVNRECADLSTVPPPPMTQQMPTQAR"
BASE COUNT          598 a         1220 c         1568 g          653 t
ORIGIN      
        1 ccgcgggtcg tcttcctcgc ggtcgcgttg gtgatggtcc ctgcgtgcag ctcatcgtca
       61 ccaacggcgg cgaccaagac caacgggacg gttggggccg tccccgcgcg accggcagga
      121 aagaacccgt cggcttcggc aaagatggtc tgcgcgaagg aagcccaaga tgcgctcgtg
      181 tccagtctcg gcgcgacgcc ggttcaggtg accacaccga cctgggtgga ccacgtgtac
      241 gcgtgtcagt acgtgtactc aaccgggacg ttcgcgctgt cggtcaagga gctcgacagc
      301 gcgaagcaga ccacggccta ctacgacggg ttcgcggcgc ggttcggccg ccggccgggt
      361 cgtctcgccg tcggccaggg cgcgtacgtg accaccgacg gttcggtggt cgtgcgcaag
      421 gactggaagg tgctgtacgt cgacgtctcg catctgccgc cgcagtttgg gcagccacca
      481 caggctgcct cggatgcggc gctcagcgtc gcggcgacga tcctcagctg ttggagcggc
      541 gcctgaccct cgtcgcgtta gctcagggga ggtggcggaa ccggagctgg agctcgaacg
      601 tgccgtggtc gccgacggag gtcacggccg tctggggcgc ctggatgccg tagtcggcga
      661 gcacgatcgg ggcggtcccg acgacgtcga tggtcgagcc gttccagcgg gcgtccaagg
      721 ccgcggtgac gtctcgggtc acgccgtgca acgtgagccg gccgggggcg ctgaggtgca
      781 gtttggtgcc gggacgaacg ctggcgggta tcgcgatggg gcttgcgagc tcgaagtggg
      841 cggtcgggaa cgtgtcggac tcgatcgcat gggtgtggat gtagtcgtcg cgtgcggcac
      901 gcccgctggt gaggttctgg agatcggcgt tgacgaccac cgacgcgact tgcccggggg
      961 tgatggtcat ggtcccgtcg acggtcggtg tccgcccgac cgcgtccttg tggatggtgt
     1021 caccggcgaa gagctcttgg atccggtagc cgacgtagac gtccttggcg cgaaggagcg
     1081 tccatgagcc caccggggtc ccagaacctg gcgcgacgtc cgtggtggcg gtcgagctga
     1141 ggtgcggctt ccccggtgag gagccgccga acacgtacca gatcaccgcg ccgacgccga
     1201 gcgccaccac gaggaacgcg gctaagagca gccaacgtag gcgaggtcgc cgcaccgggc
     1261 tcagtgctta gcggcgacga cgcggaacgc acctcgcatg aaggggtgga tgcgacagaa
     1321 gtacgtgtac gtcccgggtg cgaggttggt cggggtcgag aaggtgatgg tgccgaccgc
     1381 gggtggaccg ccgtgaccga gctcaccgga gtcgaacagc acgttggtgt tcgcgagcgg
     1441 ataggagatt cccgtcgaga ggttgcaggg ggcggaacag gacgtgaccg tatgccagat
     1501 cccgttggcg gcatcgtcgt tgcggaaggt gagcgtcccg ccctgcgcga cggcggggac
     1561 ggtggccgcc tcgctcaggt cgcccgtgta cgcgaagtcg gcgatcggga ggatgctgcc
     1621 cgaggggagg atccgcgacg gggccttgcg gacgtcctgg tagtgcttcg cgtcgggttg
     1681 gccgccgtgg ttgttgttct cggccaggtg accgtgggtg aggacgccgg acacgtcaac
     1741 cggcttttgg aacgggttga cgcccttggt cgtcccgtca gccatccaga cgaccatgat
     1801 ccccatcgac tcgtaccacg acgcgttctt cgagtcatac gtcgtcgaga tcgagagcac
     1861 gtcacccttg tggaccaccg gccgccaggt ggacggcgtc gcggtcatcg cgacgtccca
     1921 cgacacgtcg ccggctgggt cgaagtactt ggcgttcgat tggaacagcc ggacggtgtc
     1981 ggccgagccg gccttggcgt gaccggcggg cgccgtcgcg cccttgcggg tcagccacag
     2041 gctgtcgaag aggccgccgg ggtggacgtg acccgcggtg gtgaggagga caccgtcggt
     2101 ggggacggtc cattggttcc tcgccgggcc ggcgccgtag gggttcgtgg cgtcgtcggg
     2161 gtaggtgtac tggccgttgt gcccgctgcc cttgagcacg tcgaagaccg ggtagccgga
     2221 ggggttccgc acgtccatcc agatcggttg tgccgtctgc atgctggcgc tcgcgggtgc
     2281 cgtcgcgggg atgaaatcga tgtcgtacgt cagccacacc tgattcggct tcgacagctg
     2341 gttgtggagc atgtaggaga gcagccactt gtccgtggcc ttgaacgggt agccgtaccc
     2401 ggtggggagg gccatcgcgg tcttctcttc gccggcggcg aagaacctcg aattcaccag
     2461 gctcggggcc gagaggttca gccagacccc atggtggagg tggatcacgt cgaccggcgg
     2521 gacggtgccg tcggcgaggt gaaggttcgt cgtcatgccg acgatccacc cgtccgcggt
     2581 gggctcgggg atgcggcgcg agaaggagat gctgttctgg ccggggcgga tcgggagcgg
     2641 cccgacttcg aagtggagtc gttgggcgcc ggtcgggacg gggttggtca cgatcgggac
     2701 gttgctgggc ttcgcggctg cgcccggtga ggcggcggcg ggggtggcca cggtggcgac
     2761 ggcgaccagc gcggccgcag cgctcgccag caacgggagg cgctggtgca cgccatgcaa
     2821 cctaacaccg ggcgatcggc gggaactggc gggcgtccgg ccggatgccg ggtgtttcag
     2881 gttccgaagg ggggaccgtc gaagcggtcg acggtggtga actgctcgac cgcaccgcgt
     2941 gtgaggcgct tcggttggtg gtgggggtat ccgagcgcga tcaccgcggc gacggcgagc
     3001 tcgtcgggga tgccgagcaa cgctttgacg tcgggttctc ggcgaatcga catagtggtg
     3061 atcacgcctc cgagcccttc tcccctggcg gccagcagga tgctccacgc gaacgggtag
     3121 atcgatgcgg cacccgcgaa ggtgtaccgg tcgaagccct tgtcgacggc ggcgagcgcg
     3181 cgcaggtcgg cgaggagcgc gagcaggacc ggcaccgtct cgagatgctc cgcgaaacca
     3241 cccggcccgg cggccgcccg ctcggcgacg accggcgtcg tggcgaccgc ctgggcctcg
     3301 gcctcgtcgt cggtgatggg agcccacggg accaggcccg cttcgacttg ggccaggtac
     3361 tcgtaccagc cggcgaggta caggtcgcga agccgtatcc gtgtcgccgg gtcgttgacg
     3421 acgatgacgc gccagccctg gcggttgccg ccgctcggag cgaaccgcgc cgtgtcgagg
     3481 atccgatgga ggacctcgtc ggggacggga tcgccccgaa agtcgcggat cgcgcccgtg
     3541 gcgcgcagcg cctcgtcaag gtccatcatc ccaggaacgt agcgccgctc acggcgcggg
     3601 ggaccgactc ggggttcggc tatcgggcct gggtcggcat ctgctgcgtc atgggcggcg
     3661 gcggcaccgt cgagaggtcg gcgcactccc ggttcacgag ggcggtactg agcccgctgt
     3721 acgggagcgc cgggaccccg agtcggggtg gatgcgacag ctgcaacgtg ctggtgagat
     3781 ccgagaccgc gttgcgtcgc catggcgaga ggttgggtac ctcgacgccg aagcgggtct
     3841 cgaggaatcg gagcagcgag gtgtggtcga acgtgtcgga gttgatgtgg ccaccccggc
     3901 tgaacggcga gatgaccagc atgggtacgc ggaacccgag cccgatcggg ccgctgatcc
     3961 cgcccgcggc ggtcgggagt ggactggtgg tgacgaactc gtcgggggtg tcggggggtg
     4021 ggctcggtgg cgccacgtg
//
LOCUS       DATCCH010000254         2143 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_55042, whole genome shotgun sequence.
ACCESSION   DATCCH010000254 DATCCH010000000
VERSION     DATCCH010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55042"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..>2143)
                     /gene="ligA"
                     /locus_tag="VIJ44_04980"
     CDS             complement(21..>2143)
                     /gene="ligA"
                     /locus_tag="VIJ44_04980"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007075100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEY5165286.1"
                     /translation="PGERGLDEPGQPTGFVVSLGNLRVMAEPVPDTAATRVADLRREL
                     TEHTERYFVLDDPVISDAEFDDLMRELRSLEATYPALATPDSPTQRPGGAPSATFAPV
                     AHVAPMLSLDNAFTDSELRAWYTRVARIVTDPIVFVGEPKLDGLAISLLYEEGRLVRA
                     ATRGDGVTGEDVTANIATIEAVPHHLVGGSQPRRMEVRGEIFMPLAAFEDLNRRQADA
                     GDRLFANPRNAAAGSLRQKDPRVTASRALDFFAYQLGVQEGAPTLRSHHETLAWLREV
                     GVPVNPHIEVLADIEKVVEFAERMLEQRHALGYEIDGAVVKVDDLAQRAEMGSTSRAP
                     RWAIAFKFPPEEKTTRLNGIMVSIGRTGRATPFALLEPVFVGGSTVGLATLHNEDEVA
                     RKDVRPGDTVTVRKAGDVIPEVVGPVLALRRRGLRRWRFPDHCPACNARLVRLPGEAD
                     RHCVNLECPEQRVQRIVFFAGRGAMDIEGLGEERVRQFVDASLLADAGDIYALTVEGL
                     VPLERIGQKSAENLVASIEHSKSRGLARVLVGLGIRHLGPTAAQAVARSRGDLGRVER
                     ASVAELTAVDGVGPVIAESVAQFFAVEGNREVVEKLRRAGVDLSAPSPTADLPEGEAT
                     LAGRSLVITGGLERFTRDEAQAAIEARGGKVTSTVAKKTSWVIVGDSPGSKLVKAESL
                     GVPILDEDGFVQLLEHGPPEEGGP"
BASE COUNT          332 a          776 c          707 g          328 t
ORIGIN      
        1 gaccttggac ttgtcgacca tcagggacct ccttcttcgg gtgggccgtg ctctaagagt
       61 tgcacgaaac cgtcctcgtc gagaatgggg acgccgaggc tctccgcctt gaccagcttc
      121 gagccggggc tgtcgcccac aatcacccaa ctggtcttct tcgccacggt cgacgtcacc
      181 ttccccccgc gggcctcgat ggccgcctgg gcttcgtcgc gggtgaagcg ctccaggcca
      241 ccggtgatga cgagcgaccg gcccgccagc gtcgcttcac cctcggggag gtcggccgtc
      301 gggctcggcg cgctcaggtc gaccccagcc cggcgcagct tctcgaccac ctcccgattc
      361 ccctcgacgg cgaagaattg ggccacgctc tccgcgatca ccgggcccac cccatcgacc
      421 gcggtcaact cggcgaccga ggcgcgctcg acccgtccga ggtcgcctcg ggaccgggcc
      481 accgcctgcg ccgcggtcgg accaaggtgc cggatgccca ggcccaccaa cacgcgggcc
      541 agcccccgcg acttcgagtg ttcgatcgac gccaccagat tctcggccga cttctggccg
      601 atgcgttcca ggggtaccag accctcgacc gtcagcgcgt agatatcgcc ggcatccgcg
      661 aggaggctgg catcgacaaa ctggcggaca cgttcttcgc cgagcccttc gatgtccatc
      721 gcgccacggc ccgcgaagaa gacgatccgc tgcacccgct gctcgggaca ctcgagattc
      781 acgcagtggc gatccgcctc accgggcagg cggaccagcc gggcattgca cgcagggcag
      841 tggtccggaa acctccatcg ccgcaggccg cgccggcgta gggcgaggac cggtccgacg
      901 acctccggga tcacatcgcc ggccttgcgc acggtcaccg tgtcgccggg gcgaacatcc
      961 ttgcgggcga cctcgtcctc gttgtgcagc gtggcgagcc cgacggtcga gccgccgacg
     1021 aacaccggct cgagcagcgc gaacggcgtc gcccgaccgg tccgaccgat gctcaccatg
     1081 atcccgttca gacgggtggt cttctcctcc ggagggaact tgaacgcgat cgcccaccgg
     1141 ggagcgcggc tcgtcgagcc catctcggcc cgttgggcca ggtcgtcgac tttcaccacc
     1201 gccccgtcga tctcatagcc gagggcgtgg cgttgctcga gcatccgctc ggcgaactcg
     1261 accaccttct cgatgtcggc caacacctca atgtgcggat tcaccggcac gccgacctct
     1321 cggagccacg ccagcgtctc gtgatgcgac cgcagcgtcg gtgcaccctc ttggacgccg
     1381 agctgatagg caaagaagtc gagcgcccgc gacgcggtga cgcgtgggtc cttctggcga
     1441 agacttcccg cggcggcgtt ccgcgggttc gcgaacaggc gatcacctgc gtcggcctgg
     1501 cgccggttga gatcctcgaa tgccgcgagc ggcatgaaga tctcaccccg cacctccatc
     1561 cggcgaggct gcgagccgcc gacgagatgg tgtggcaccg cctcgatcgt ggcgatattc
     1621 gccgtgacgt cctcgccggt cacaccgtcg ccgcgggtcg cggctcggac gaggcggcct
     1681 tcctcgtaga ggagcgagat cgcgagccca tccagcttcg gctcgccgac gaacacgatc
     1741 gggtcggtga cgatccgagc aacccgcgtg taccaggccc gcagctccga gtcggtgaac
     1801 gcgttgtcga gtgacagcat cggggcgacg tgcgccacgg gtgcgaacgt ggctgagggc
     1861 gccccgcccg ggcgctgggt cggcgagtcc ggcgtcgcga gcgcgggata ggtcgcctcg
     1921 aggctccgca gctcgcgcat caggtcatcg aactcggcgt cggagatcac gggatcgtcg
     1981 agcacgaaat agcgttcggt gtgctccgtg agctcgcgcc gtaggtcggc gacgcgcgtc
     2041 gccgcggtgt cggggacggg ctcggccatc actcgcaggt taccgagaga gaccacaaat
     2101 cccgtgggct gaccaggctc gtcgagaccg cgctcgcccg gcc
//
LOCUS       DATCCH010000255         2577 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_484915, whole genome shotgun sequence.
ACCESSION   DATCCH010000255 DATCCH010000000
VERSION     DATCCH010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484915"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VIJ44_04985"
     CDS             <1..251
                     /locus_tag="VIJ44_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007166771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEY5165287.1"
                     /translation="DMVGMPPHHELLAHYEAVSGRSTEDIDYYEILARFKLAIVLEAQ
                     YARYRQGGADNPKIEAFGPITLDLMAKAAEHTARAGVS"
     gene            complement(282..2246)
                     /locus_tag="VIJ44_04990"
     CDS             complement(282..2246)
                     /locus_tag="VIJ44_04990"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY5165288.1"
                     /translation="MTTIDTSQPPSDARKVGALKPDLLLTPEHHLCPGCGEPLALRCL
                     LDTITELGVAARTVGVVGIGCYTAFSMTLDVDLVQALHGRAPSVATGLKRVQPDSVVF
                     TLQGDGDMVNEGLQEVLHTAARGEAVTCVLLNNGVFGETGGHMTATTVLGHRTKNTLD
                     GRDADYHGYPILIGDLISRLEGAAYVARGSVHNAGAVARTKKMVRRALECQTRREGFS
                     FVEVLTMCPTGWFVPTHDGPQYLHDTLGEVHVMGELKVDGHIRTTEELHAENIARQRE
                     IEARLAAGGLDDHVTAETRSLGLATGLERGSDALERVRAWNRELADAGYAAISWPAEY
                     GGRGAGVMDQVVWNEEMHRSGAPGPINVIGIPNVAPAIMSYGTESQKAALLPPMRRGD
                     EIWCQGFSEPDAGSDLASLRTSAVRDGDDWVIAGQKIWTTLGQLADWCELLVRTDPDA
                     PKHRGITCVLVDMDRAGVEVRPLTTITGEHEFNEVFFDDVRVPVDQTLGPVNEGWRVA
                     MTTLAHERGGVAKLHLGLRAKVARLLDTARRTPLGDGRMASDDPVLRARLARAYLHAE
                     LLKLISDRAISGELHGRSLGPEGSIAKLQWSETEQLVAEVAGDVLGPDANSGEWGRDR
                     VMARSHTIAGGTTQVNKNIIATRILGLPRG"
     gene            complement(2258..>2577)
                     /locus_tag="VIJ44_04995"
     CDS             complement(2258..>2577)
                     /locus_tag="VIJ44_04995"
                     /inference="COORDINATES: protein motif:HMM:NF028456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165289.1"
                     /translation="GERIGFVRPITLWPFPSAPIAAAAASARTVAVFELCAGQMIDDV
                     RLAVLGQAPVVGIGGISTDGSGFGVGRLLDTEIIRERLLAVHRRGADAELPPLPDNER
                     ALW"
BASE COUNT          391 a          857 c          928 g          401 t
ORIGIN      
        1 tcgacatggt gggcatgcca ccccaccatg agctcctcgc gcactacgaa gcggtgagcg
       61 gccgctcgac cgaggacatc gactactacg agatcctcgc ccggttcaag ctcgccatcg
      121 tcctcgaggc gcaatatgcc cgctaccgcc aaggtggcgc cgacaacccc aagatcgagg
      181 cgttcgggcc gatcaccctc gacctgatgg cgaaagccgc cgagcacacg gcccgagctg
      241 gcgtgagctg agcccgactc cccgagttgc gggtcgcgac gtcagccgcg aggcagtccg
      301 agtatccggg ttgcgatgat gttcttgttc acctgggtgg taccgccggc gatggtgtgc
      361 gagcgggcca tgacccggtc tcggccccat tcaccgctgt tggcgtcggg cccgagcacg
      421 tcgccggcga cctcggcgac gagctgctcg gtctcgctcc actggagctt ggcgatgctg
      481 ccttcggggc cgagggaccg gccgtggagc tcgccggaga tggcccggtc agagatgagc
      541 ttcaacagct cggcgtggag gtaggcgcga gcaaggcggg cgcgcaagac tgggtcgtcg
      601 ctcgccatgc gaccgtcgcc gaggggagtc cggcgggcgg tgtcgagcag ccgcgccact
      661 ttggctcgca gaccgaggtg gagcttggcg acgccgcctc gctcgtgggc cagggtggtc
      721 atcgccacgc gccaaccttc gttcaccgga ccgagggtct ggtcgaccgg gacgcgcacg
      781 tcgtcgaaga acacctcgtt gaactcgtgt tctcccgtga tcgtggtgag gggacggacc
      841 tcgacgccgg cccggtccat gtcgacgagg acacaggtga tacctcggtg cttgggggcg
      901 tcggggtcgg tgcggacgag gagctcgcac cagtcggcga gctgaccaag ggtggtccag
      961 atcttctggc ccgcgatcac ccagtcgtca ccgtcgcgca ccgcgctggt gcgcagcgac
     1021 gccaggtccg atcccgcgtc gggttcggag aacccttgac accagatctc atccccgcga
     1081 cgcatcggag gcaacagcgc agccttttgc gattcggtcc cgtagctcat gatcgcgggg
     1141 gcgacgttgg ggatcccgat gacgttgatc ggtccgggcg cgccgctgcg atgcatctcc
     1201 tcgttccaca cgacctggtc catcaccccg gcgccgcggc ccccgtactc ggccggccag
     1261 gagatcgcgg cgtagccggc gtcggcgagc tcgcggttcc aggcccggac ccgctccagc
     1321 gcatcggatc ctcgttcgag accggtggcc agaccgaggc tgcgggtctc ggcggtgacg
     1381 tggtcgtcga gcccgccggc ggcgagccgg gcctcgatct cgcgttggcg ggcgatgttc
     1441 tccgcgtgga gctcttcggt ggtccgaatg tgaccgtcga ctttcagctc gcccatcacg
     1501 tgcacctcgc cgagggtgtc gtggaggtac tgcgggccgt cgtgggtcgg gacgaaccag
     1561 ccggtagggc acatggtgag gacctcgacg aacgagaagc cctcccggcg ggtttggcac
     1621 tcgagggccc ggcggaccat cttcttggtg cgtgccaccg cgccggcgtt gtgaaccgag
     1681 ccacgggcga cataggccgc gccctcgaga cgcgagatca gatccccgat gaggatcggg
     1741 tacccgtggt agtcggcgtc gcgcccatca agggtgttct tggtgcgatg accgagcacg
     1801 gtcgtcgcgg tcatgtggcc gccggtctcg ccgaacacac cgttgttgag caggacacag
     1861 gtgacggcct caccgcgcgc cgcggtgtgc agcacctctt ggaggccttc gttgaccatg
     1921 tcgccgtcac cctggagggt gaagacgacc gagtccggtt gcacccgctt gagcccggtc
     1981 gccaccgacg gggcccgacc gtgaagcgcc tggacgagat cgacatcgag ggtcatcgag
     2041 aacgcggtgt agcagccgat gccgacgacg ccgacggtgc gcgcggcgac ccccagctcg
     2101 gtgatggtgt cgagcaggca gcgcaacgcg aggggctcac cgcagcccgg gcagagatga
     2161 tgctcggggg tgaggagcag gtcgggcttc agggccccga ccttgcgggc gtcgctcggg
     2221 ggctgagacg tgtcgatggt ggtcaccggt cctcctgtta ccacaaggcg cgctcgttgt
     2281 ccggcagtgg cggcaactcg gcgtcggcgc cgcggcgatg cacggctagc agccgttccc
     2341 ggatgatctc ggtgtcgagg agccgaccga cgccgaaacc ggacccgtcg gtcgagatcc
     2401 cgccgatccc gaccaccggg gcctggccga ggaccgcgag gcgcacgtcg tcgatcatct
     2461 gcccggcgca gagttcgaag accgcgaccg tgcgcgccga ggcggccgcg gcagcgatcg
     2521 gcgccgacgg gaacggccag agggtgatgg ggcggacgaa gccgatccgc tcgccgg
//
LOCUS       DATCCH010000256         4468 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_138351, whole genome shotgun sequence.
ACCESSION   DATCCH010000256 DATCCH010000000
VERSION     DATCCH010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4468
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138351"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1186
                     /locus_tag="VIJ44_05000"
     CDS             <1..1186
                     /locus_tag="VIJ44_05000"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEY5165290.1"
                     /translation="LIVGAATDVGRVRGHNEDAYLVDEQLGLVAVADGMGGHQAGEVA
                     SATALEALRAAVTSGEGIRDAVTSANAAVYDKSTTDERLHGMGTTLTAATLATGATLL
                     LGHVGDSRAYLLHDGKLQRLTTDHSLVEELIQSGELTPEEAEADPRRSMITRALGIEP
                     DVDVDLYPTQLSSGDRLLFCSDGLTGMVSEEEIQTILADDHDPSAGARRLVAAANTAG
                     GIDNITVLVVDVVDDEGPTTAVPTVTAAEEPTEEPPHPTGEPSPTVARRQGRRRRLWR
                     IVRWGLPIVVVVGVAIGVLYWYARNTYYVGTDLGNVAIFRGRPGGVLVWNPTIVGKRT
                     EIVANDLPPRLRQEVRGGHGQFSSANDARAYVRRLSASTTTTTTVTTPATTTPTAPKP
                     AP"
     gene            1192..2505
                     /locus_tag="VIJ44_05005"
     CDS             1192..2505
                     /locus_tag="VIJ44_05005"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEY5165291.1"
                     /translation="MPAANRRGAELGLGLLAVIVAAFGYLLVQLAQKPDVPPDLWVIF
                     AAVLALFVVAHLAVRRLAPDANQVLLPIAALLNGLGFVTVARLDSNLGRIQAAWTAVS
                     VLAFVLTLVGVRRIRDLERYRYSFLFLGMVALLLPLAPGLGKTINGARLWVGVGPVNF
                     QPGEAAKVFLVLFFAAYLVDKRELLASGGRRIGRMMLPEPKHLGPLLLAWGVSILIMV
                     RETDLGQAMLIFALCGVMLYVATERAAYTVVTVVLFVTAAGIAYQLFGHVQTRVQIWI
                     DPFKYANDKGYQLVQSLFGFAAGGFAGTGLGLGSPKQIPNVATDFVFSAIGEELGLLG
                     CVAVLVALLLFVGSGYQIAVRSERPFPKLFATGLTTIIGIQSFVIIGGVTRVIPLTGV
                     TLPFISYGGSSLLANWVILALLLRISDDNAQGTQARRPGRTARAP"
     gene            2502..3965
                     /locus_tag="VIJ44_05010"
     CDS             2502..3965
                     /locus_tag="VIJ44_05010"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEY5165292.1"
                     /translation="MNRAIRRVGIGIVALVLILVAQLTYLTIVDANNLKHNPHNVRAL
                     LANANRPRGDIVTADGTIVAQSRTVSDGTVFKYLRQYPTKELFAQTVGYQSFVFGSTG
                     VENSYNNALVGRENQLNLNNIPDILSGKQSTGTVVLSLRADAQRLAQSALGAQRGSVV
                     LLDVKTGAVLAMYSNPSFDPNPLVVHNNQLAQAAFTAVQQNPDHPALPRSYRERYPPG
                     SSFKTITASVALEDGVATPNSTFPVRTELPLPQSTNTLRNFGGESCGGTLTLSFTQSC
                     NTTFGQLGLQLGDQFVPGMAKFGIGQAPPIDLRPGAVTSVGPASGTFSNNQPQFALAG
                     IGQGAVAVTPLQMALVAEGIANGGVIMQPHAASEIRDANDKLIRKINPSPWMTATTPQ
                     VAQAVTQMMIQVVNSGTGTAAQIPGVTVAGKTGTAQTAPNQAPHAWFVAFAPAEAPRF
                     AVAVIVEHGGNASQEATGGRVAAPIAAQMLKLALGVK"
     gene            4076..4276
                     /locus_tag="VIJ44_05015"
     CDS             4076..4276
                     /locus_tag="VIJ44_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165293.1"
                     /translation="MVVFADRDLTAREFDELADAVHKWMYPTDPPEAWLSPDRVISSE
                     TPRPIARGGTVSPIVTTTLIRR"
     gene            4273..>4468
                     /locus_tag="VIJ44_05020"
     CDS             4273..>4468
                     /locus_tag="VIJ44_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012946288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEY5165294.1"
                     /translation="MTQGEGTFANRYTVERPIARGGMADVYLARDQVLDRLVAVKVLF
                     PEFARDPSFVERFRREAHSAA"
BASE COUNT          701 a         1614 c         1433 g          720 t
ORIGIN      
        1 gctcatcgtc ggtgccgcca ccgatgtcgg gagggtccgt ggtcacaacg aggacgcgta
       61 cctcgtcgac gaacagctgg gccttgtcgc cgtcgccgac ggcatggggg gacaccaggc
      121 gggcgaggtc gcgagcgcca ccgcgctcga ggccctacgc gccgcggtca cctcagggga
      181 gggcatccgc gacgcggtca cgtcggccaa cgcggcggtg tacgacaagt cgaccaccga
      241 tgaacgcctg cacgggatgg gtacgacgct caccgcggcg acgctcgcca ccggtgccac
      301 gctccttctc ggccacgtcg gtgactcccg cgcgtacctc ctgcacgacg ggaagctaca
      361 gcgcctcacc accgaccaca gcctggtcga ggaactcatc caatcggggg agctcacccc
      421 cgaggaggcc gaagccgacc ctcgccgctc catgatcacc cgcgccctgg gaatcgaacc
      481 cgacgtcgac gtcgacctgt acccgaccca gttgtcgagc ggcgaccgcc tgctgttctg
      541 ctccgacggg ttgacgggca tggtctccga ggaggagatc cagacgatcc tcgccgacga
      601 ccacgatccc agcgccgggg cccgacgcct cgtcgcggcg gcgaacaccg ccggcggcat
      661 cgacaacatc accgttctcg tcgtcgatgt cgtcgacgac gaggggccca ccaccgcggt
      721 tcccaccgtc accgccgcgg aagaaccaac cgaagagccg ccccatccga ccggggagcc
      781 gtcacccacg gtggcccgcc gccagggccg gcggcgacga ctctggcgga tcgtgcgctg
      841 gggactcccg atcgtcgtcg tcgtcggcgt cgcgatcggc gtcctctact ggtacgcgcg
      901 gaacacgtac tacgtcggga ccgacttggg gaacgtggcg atcttccggg gccgcccagg
      961 cggcgtgctc gtctggaacc ccacgatcgt ggggaagcgc accgagatcg tggcgaacga
     1021 cctgccgccc cgcctacgcc aggaagtgcg gggcgggcac ggccagttct cctcggccaa
     1081 cgacgcccgg gcctacgtcc gccggctgag cgcctcgacg acgacgacga ccacggtcac
     1141 gacaccggcg actaccaccc cgactgcgcc gaaacccgcc ccgtgagcgg cgtgccggct
     1201 gccaatcgcc gcggcgcgga gctcggcctc gggctgctgg ccgtcatcgt cgccgcgttc
     1261 ggctacctcc tggttcagct cgcccagaag cctgatgtcc cccccgacct ctgggtgatc
     1321 ttcgccgcgg tcctcgcctt gttcgtcgtc gctcacctgg cggtccggcg tctcgctccc
     1381 gacgccaacc aggtgctgct ccccatcgcc gcgctgctga acggcctggg gttcgtcacc
     1441 gtcgcccgcc tcgacagcaa cctcggtcgc atccaggccg cgtggactgc ggtgagcgtc
     1501 ctggcgttcg tgctgaccct cgtcggcgtc cgacgaatcc gcgacctcga gcggtaccgc
     1561 tactccttct tgttcctcgg gatggtcgcg ctgttgctcc cgctcgcccc cgggctcggc
     1621 aagacgatca acggagcccg gttgtgggtg ggcgtggggc cggtgaactt ccagcctggt
     1681 gaggcagcca aggtgttcct cgtcttgttt ttcgccgcgt acttggttga caagcgcgag
     1741 ctgctggcga gcggcggtcg ccgcatcggc cggatgatgc tgcccgagcc caagcatctc
     1801 ggcccgctgc tgctcgcttg gggcgtctcg attctgatca tggtgcgcga gaccgatctc
     1861 ggtcaggcga tgctcatctt cgcgttgtgc ggcgtgatgc tgtacgtcgc caccgagcgt
     1921 gccgcctaca cggtggtgac cgtcgtgttg ttcgtgaccg ccgccggcat cgcctaccag
     1981 ctattcggtc acgtccagac ccgagtgcag atctggatcg acccgttcaa gtacgccaac
     2041 gacaagggct atcagctcgt ccagtccttg ttcggattcg cggcgggcgg gttcgcggga
     2101 accgggctcg gcctcgggag cccgaagcag atacccaacg tcgcgaccga cttcgtcttc
     2161 tccgccatcg gcgaggagct cgggctcctg gggtgcgtcg ccgtgctggt ggccttgctc
     2221 ctcttcgtcg ggtcgggcta ccagatcgcc gtacgctccg aacggccgtt cccgaagctg
     2281 ttcgccaccg gcctcacaac gatcatcgga atccagagct tcgtgatcat cgggggtgtc
     2341 acccgggtca tcccgctcac cggcgtcacg ctgccgttca tctcgtacgg gggctcctcg
     2401 ttgttggcca actgggtgat cttggccctg ctgctgcgga tctcggacga caacgcccaa
     2461 ggcacccagg cgcggcggcc ggggagaacc gcccgcgcac catgaaccgg gctatccgcc
     2521 gagttggaat cgggatcgtc gcgctggtcc tgatcctcgt cgcccagctg acctacctga
     2581 cgatcgtcga cgccaacaat ctcaaacaca atccgcataa cgtgcgggcg ctgctggcga
     2641 acgccaaccg tccccgcggt gacatcgtca ctgccgacgg gacgatcgtc gcgcagtcgc
     2701 gcacggtgtc tgacggcacg gtcttcaagt acctgcgcca gtaccccacg aaggagctgt
     2761 tcgcccagac cgttggctac cagtcgttcg tgttcggtag cacgggggtc gagaactcct
     2821 acaacaacgc gctggtcggc cgcgagaacc aactgaacct gaacaacatc cccgacatct
     2881 tgtccggcaa gcagtccacc ggcaccgtcg tgctgtcgtt gcgcgccgac gcgcagcggc
     2941 ttgcgcaatc cgcactggga gcccagcggg gctcggtggt cttgctcgac gtcaagaccg
     3001 gggccgtctt ggcgatgtac tcgaacccgt cgttcgaccc caatcccttg gtcgtgcaca
     3061 acaaccagct cgcgcaggcc gcgttcaccg cggtgcaaca gaaccccgac catcccgcgt
     3121 tgccgcggtc ctaccgcgag cgctacccgc ccggttccag cttcaagacg atcacggcca
     3181 gcgttgcgct cgaggacggg gtggcgaccc cgaacagcac gttccccgtc cgcaccgagc
     3241 tcccgctgcc tcagtcgacg aacacgctga gaaacttcgg cggcgagtcg tgtgggggga
     3301 cgttgacctt gagcttcacc cagtcgtgca acacgacgtt cgggcagctg ggcctgcagc
     3361 tcggtgacca gttcgtgcct ggcatggcga agttcggtat tgggcaggcg cctcccatcg
     3421 acctgcgccc cggcgccgtg acgagtgtcg gaccggcctc cggcaccttc agcaacaatc
     3481 agccgcagtt cgccctggcc gggatcggcc aaggtgccgt ggcggtcact cccctgcaga
     3541 tggcgctggt cgccgaggga atcgccaacg gcggcgtgat catgcagccc cacgccgcca
     3601 gtgagatccg cgacgcgaac gacaagctca tccgcaagat caacccgtcg ccgtggatga
     3661 cggcgacgac cccccaggtt gcccaggcgg tcacccagat gatgatccag gtcgtgaaca
     3721 gcggaaccgg taccgcggcg cagattccgg gcgtcaccgt cgccggcaag accggcaccg
     3781 cccagaccgc gcccaaccag gcgccccacg cctggttcgt ggcgttcgcg ccggccgagg
     3841 caccccgctt cgcggtggcg gtcatcgtcg agcacggagg caacgcgagc caggaggcca
     3901 ccggcggtcg cgtcgcagcc ccgatcgcag cgcagatgct gaagctcgca ctcggcgtga
     3961 agtagtcggc cgtcagccag acgggtgtcc ccgcacgatc acggggtggc gttcacgttc
     4021 cgcgccgtgc gcgacgttcg aggagcaatg ggacactcgc ggcgacgcac acctcgtggt
     4081 ggtgttcgcc gatcgcgacc tcaccgctcg ggagttcgac gagctcgccg acgccgtgca
     4141 caagtggatg tacccgaccg acccgcccga agcgtggcta agccctgacc gcgtcatctc
     4201 ttcggagaca ccgcgaccaa tcgcgcgcgg tggcaccgtt tcgccgatcg tgaccactac
     4261 cctgatccgt cgatgacgca aggcgagggc accttcgcga accgctacac cgtcgagcgc
     4321 ccgatcgccc gaggtgggat ggcggacgtt tacctcgccc gcgaccaggt gctggaccgc
     4381 ctcgtggccg taaaggtcct ctttcccgag ttcgcgcgcg acccgagctt cgtcgagcgg
     4441 ttccgccgtg aagcgcacag cgcggcgt
//
LOCUS       DATCCH010000257         2501 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_387841, whole genome shotgun sequence.
ACCESSION   DATCCH010000257 DATCCH010000000
VERSION     DATCCH010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387841"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="VIJ44_05025"
     CDS             <1..467
                     /locus_tag="VIJ44_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165295.1"
                     /translation="MAERPLWDWGLHLARDVRTPAQISRVLPVLLGAVIPNLVAEPRA
                     FIRSARLARDADLTVALDVLRRRGLPVVVVWGRRDGVVTDASFRALCSALGDAATITV
                     PGGHNWLLVDPDRFGEVMTNVVEVARQSRWLEPVGPMRRVWRRVVRRRRRRR"
     gene            464..1516
                     /locus_tag="VIJ44_05030"
     CDS             464..1516
                     /locus_tag="VIJ44_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005628708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase"
                     /protein_id="HEY5165296.1"
                     /translation="MSRAPLRVIQWTTGNVGRRALRAVVRHPNLELVGVFAHTSDKAG
                     RDAADLCGADEDTGVVATTDVDALLALSADACSYNPMWPDTDTLCALLESGVNVCSTA
                     GFITGRATGEETVRRLLTAAERGRSTMFGTGINPGFANLFALLSTQICDRIEQIRVLE
                     SADASTYESKHTQESVGFGHRLDALDLLERTRAGSAVFADAVAMMADGLDVELDAITF
                     DADYAAATADNDLGFMVIPEGTVAAIDGRWRGRVGDRDLIVLNYQWIMGRHVEAPFPL
                     RHGYFVEIDGEPGVRAQLQIVPPPDWNEPDYMGLGMIMTAMPAVNAIPAVVAAPPGIA
                     THLTLPLVTARGFADT"
     gene            1773..>2501
                     /locus_tag="VIJ44_05035"
     CDS             1773..>2501
                     /locus_tag="VIJ44_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEY5165297.1"
                     /translation="MESTHARARPRRRRGPVLAAVLGGLLVTLGVTSPAGASGATRPA
                     AKAAARINVVVAENFWGSIVKQLAGSHGDVTSIVTNPATDPHSYEPKPSDSRAIAGAR
                     YVVVNGIGYDPWAQQALEANPSSGRKVLVVGDLLGLKDGDNPHQWYSPDSVERFIDRV
                     TGDLERLDPRDARAFDAQRSQFETVGLKQYRAVIASIRQKYAGTPVGASESIFSPLAD
                     GLGLKVLTPPSFLKAIAEGTDPSAA"
BASE COUNT          392 a          881 c          835 g          393 t
ORIGIN      
        1 cgatggcgga gcggccacta tgggactggg gtttgcactt ggcgcgcgac gttcggacgc
       61 cggcacagat ctctcgggtc ttaccggtcc tgctcggtgc cgttatcccg aacctcgtcg
      121 cggagccgcg cgcgttcatc cggtcggccc gcctggcgcg cgacgcggat ctcacggtcg
      181 cactcgatgt gctccgacga cgcggtcttc ccgtcgtggt ggtgtgggga cgtcgcgacg
      241 gggtggtcac ggacgcctcg ttccgagcgc tgtgctcggc gctcggcgac gccgccacca
      301 tcaccgtccc gggcggccac aactggctgt tggtcgaccc ggaccggttc ggtgaggtga
      361 tgaccaacgt cgtcgaggtg gcccggcaat cccggtggct cgaacccgtc ggcccgatgc
      421 gacgggtctg gcgccgcgtc gttcgccgtc gtcgtcgtcg tcgatgagca gagctccgct
      481 gcgcgtcatc cagtggacga ccggaaacgt cggacggcgg gcgcttcgcg ccgtcgtgcg
      541 tcacccgaat ctcgagctcg tcggggtgtt cgctcacacc tcggacaagg caggacggga
      601 cgcggctgat ctctgtgggg cggacgagga caccggagtg gtggcgacga ccgacgtcga
      661 cgcgctcctc gccttgagcg cggatgcctg ctcgtacaac ccgatgtggc ccgatactga
      721 cacgctctgc gctctactcg aatccggtgt caacgtctgc tcgaccgccg ggttcatcac
      781 cggtcgcgcg acgggcgagg agaccgtacg gcgtctcctc acggccgccg aacgcggtcg
      841 ctccacgatg ttcggcaccg gaatcaaccc gggcttcgcc aacctgttcg cgctgctcag
      901 cacccagatc tgtgaccgga tcgagcagat ccgggtcctc gaatcggctg acgcgtcgac
      961 gtacgagtcg aagcacaccc aggagagcgt cgggttcggg caccgtctcg acgctctcga
     1021 cctgctggag cggacccggg ccggcagcgc ggtcttcgcc gacgcggtgg ccatgatggc
     1081 cgatgggctc gacgtcgaac tcgacgcgat cacgttcgac gccgactacg cggctgcgac
     1141 tgcggacaac gatctgggat tcatggtcat ccccgaagga acggtggcgg cgatcgacgg
     1201 ccgctggcgc ggtcgagtcg gggaccgaga cctgatcgtc ttgaactacc aatggatcat
     1261 ggggcgccat gtcgaggcac cgttcccgct ccgacacggg tatttcgtcg agatcgacgg
     1321 tgaaccgggg gtccgcgctc agctccagat cgtcccaccg cccgactgga acgaacccga
     1381 ctacatgggc ctcgggatga tcatgacggc catgccggcg gtcaacgcga tcccagccgt
     1441 cgtcgccgca ccgcccggaa tcgcgaccca cctgacgctg ccgctggtca ccgcgcgcgg
     1501 gttcgccgac acctgagctc gcggaccgcc ccgcgtcgag aagccgggcc gccgaccgcc
     1561 gatgccgcga ggccaagccc tcgtcgatgt gacgcatcgg gcgctgcgct agcgccaacc
     1621 cgaccgcagc gggatctggt tttccgtagc ccatgaccat gaacgtgggc tcggtttctc
     1681 cgaccgtttg gcgcggcgct cgcgccaaac ttgtaatggg aaccgttctc acatagcctc
     1741 gggggtcact cgcggaggag gccaacggga gagtggagtc cacccatgcg cgagcgaggc
     1801 cccgacgtcg ccgcgggccc gtcctcgccg ccgtccttgg gggcctgctg gtcacgctcg
     1861 gggtcaccag ccctgccggc gcgtcgggtg ccactcgacc ggcggcaaaa gcggcggccc
     1921 ggatcaacgt cgtcgtggcc gagaacttct ggggcagcat cgtcaagcag ctcgccggct
     1981 cgcacggcga cgtcacgagc atcgtcacga acccggccac cgacccacac tcctacgagc
     2041 cgaagcccag cgacagccgt gcgatcgcgg gcgcgaggta cgtcgtcgtc aacgggatcg
     2101 gctacgaccc gtgggcgcaa caggcgctcg aagcgaaccc cagctcaggc cgcaaggtgc
     2161 tcgtcgtcgg tgacctgctc ggcctcaagg acggcgacaa cccgcaccag tggtactcac
     2221 cggacagcgt cgaacggttc atcgatcgcg tcaccggcga tctcgaacgg ctcgaccccc
     2281 gagacgcccg cgccttcgac gcgcagcgca gccagttcga aaccgtcgga ttgaagcagt
     2341 accgcgcggt catcgcgtcg atccgccaga agtacgcggg aaccccggtc ggcgcctcgg
     2401 agagcatctt ctctcccctc gctgatggtc tgggcctcaa ggtcctcact cccccgtcgt
     2461 tcctcaaggc gatcgccgag gggaccgacc ccagcgcggc g
//
LOCUS       DATCCH010000258         2267 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_428938, whole genome shotgun sequence.
ACCESSION   DATCCH010000258 DATCCH010000000
VERSION     DATCCH010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428938"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VIJ44_05040"
     CDS             complement(<1..203)
                     /locus_tag="VIJ44_05040"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018655719.1"
                     /note="catalyzes the formation of
                     N-succinyl-LL-2,6-diaminopimelate from
                     N-succinyl-L-2-amino-6-oxopimelate in lysine biosynthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HEY5165298.1"
                     /translation="MSAPDPATGFVPPPYPHDRLTDVREVAAAVPGGLIDCSVGTPVD
                     PFPDVARRALDAAMGTARGYPPT"
     gene            complement(233..1537)
                     /gene="hflX"
                     /locus_tag="VIJ44_05045"
     CDS             complement(233..1537)
                     /gene="hflX"
                     /locus_tag="VIJ44_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEY5165299.1"
                     /translation="MSSPDGRARRRLTATEIDLEVLRQRALLVGTGVGSRHLDESEAS
                     LAGLALLADTAGADPVHSVLQRRDHPDPATYVGSGKVDELSSLAESLDIDVVVFDDEL
                     TPAQQRNLEHRLGRDVVDRVALILDIFAQHATSQEGMLQVELAQLRYRMPRLRGRGIT
                     LSQQGGGIGTRGPGETQLEVDRRRIQRRITKLEQDLKRLGRTRATQRKARRRRGLPAV
                     ALVGYTNAGKSTLLNRITDAEVRVEDRLFSTLDPTTRRLRLTGGETVLCSDTVGFVRR
                     LPHELVEAFRSTLEMVTEADLILHVVDGAAPDAPAQIAAVHTVLDEIEATDVPELLIV
                     NKVDIAEPARVRELCQGETALAVSAATGEGVDKLLSAVGDRLRALATVVELLVPYERG
                     DVLAGLHRDGEVLVEVHVDAGTRVRARIDGAALSRYREFVTG"
     gene            complement(1534..>2267)
                     /gene="dapF"
                     /locus_tag="VIJ44_05050"
     CDS             complement(1534..>2267)
                     /gene="dapF"
                     /locus_tag="VIJ44_05050"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEY5165300.1"
                     /translation="RRRGVGADGLMVLGPGDHEVDCTMTLFNADGGPAEMSGNGIRCL
                     AWVAVQEGLARDGRLMVRTAAGSRIVDLTLDPTTGDLRSATVDMGPVTFDPAGIPLDA
                     ASAFDLEAGYHGVTYHGDAAGVGNPHLVLLVDDVEAARVTQHGPHLEHDPRFLNGTNV
                     EFVAVGGPDELRMRVWERGVGETLSCGTGACAAAAVAHRRHLVGPEVTVHVRGGDLSV
                     HLGDTARLGGPVVHVFDVDLNGPST"
BASE COUNT          316 a          720 c          892 g          339 t
ORIGIN      
        1 atcgtgggcg ggtaaccgcg tgctgttccc atggccgcgt cgagggcacg gcgcgccacg
       61 tccgggaaag ggtcgaccgg cgtaccgacc gagcagtcga tcagtccgcc ggggaccgcg
      121 gcggcgacct cgcggacgtc cgtcaggcga tcgtgggggt acgggggtgg gacgaagccg
      181 gtcgccgggt caggagccga catgagagca ggctaccgcc gggtgttgac ggtcagccgg
      241 tgacaaattc gcgataccga ctgagggcgg cgccgtcgat tcgggcccgc acccgggtgc
      301 cggcgtcgac gtgcacttcg acgaggacct caccatcgcg gtggaggccc gccaggacgt
      361 cgccccgctc gtagggcacc aggagctcga cgacggtcgc gagggcacga aggcggtccc
      421 cgaccgcgct gagcagcttg tccacgcctt cgccggtcgc ggccgagaca gccagggcgg
      481 tctcgccctg gcagagctca cgcacccggg cgggctcggc gatgtcgacc ttgttgacga
      541 tgagcagctc cggcacgtcg gtcgcctcga tctcgtcgag gacggtgtgg accgcggcga
      601 tctgggccgg cgcgtcgggt gcggctccgt cgacgacgtg gaggatgaga tccgcttccg
      661 tgaccatctc gagggtggag cggaacgctt cgacgagctc gtgggggagg cgtcggacga
      721 agccgacggt gtccgagcac aggaccgtct cgccgccggt gagccggagg cgtcgggttg
      781 tcggatcgag ggttgagaag agtcggtcct cgacgcgtac ctcggcgtcg gtgatgcggt
      841 tgagcagggt ggatttgccg gcgttggtgt acccgacgag cgcgacggcg gggaggcccc
      901 gacgccgtcg agccttgcgt tgggtggccc gggtccgtcc gagccgcttg aggtcctgtt
      961 cgagcttggt gatgcgccgt tggatgcgac gccgatcaac ttcgagctgg gtctcaccgg
     1021 gaccgcgggt gccgatgccg ccgccctgct ggctgagggt gatgccccgg ccccgcagtc
     1081 gcggcatccg gtagcgcagc tgcgccagct cgacttggag catgccttct tggctggtgg
     1141 cgtgctgggc gaagatgtcc aggatgagcg ccactcggtc cacgacgtcg cgtccgaggc
     1201 ggtgttcgag gttgcgctgc tgggcgggcg tgagctcgtc gtcgaagacg accacgtcga
     1261 tgtcgaggga ttcggccagg ctgctcagtt cgtcgacctt gccggacccg acgtaggtgg
     1321 ccgggtcggg atgatcccgc cgctgcagca cggaatgcac ggggtccgcg ccggcggtgt
     1381 cggcgaggag cgccaaccca gcgagtgacg cctcggattc gtcgaggtgc cggcttccga
     1441 cgccggtgcc gacgaggagc gcgcgctggc gcaggacctc gaggtcgatt tcggtggcgg
     1501 tgaggcgacg acgggcgcgg ccgtcgggac tgctcacgtg ctgggtccgt tcaggtcgac
     1561 gtcgaagacg tgcacgacgg gaccgccgag ccgggcggtg tcgccgaggt gcaccgagag
     1621 gtcacccccg cggacgtgga ccgtgacctc cgggccgacg aggtggcgtc ggtgggcgac
     1681 ggctgcggcg gcgcacgcac ccgttccgca cgagagcgtc tcaccgacgc cgcgttccca
     1741 gacccgcatc cggagctcgt cggggccgcc gacagcgacg aactcgacgt tggtgccgtt
     1801 caggaaccgg ggatcgtgct cgaggtgggg tccgtgctgg gtgacgcggg ccgcctcgac
     1861 gtcgtcgacc aacaggacca ggtgcgggtt gccgacgccg gcggcgtcac cgtggtaggt
     1921 gacgccgtga tagccggctt cgagatcgaa ggcgctggcc gcgtcgaggg ggatgccggc
     1981 cgggtcgaac gtcaccgggc ccatgtcgac agtggcggaa cggagatcgc cggtcgtggg
     2041 gtcgagggtg aggtcgacga tgcgggagcc ggcggcggtg cgaaccatga gccggccgtc
     2101 gcgggccaga ccctcctgga ccgcgaccca ggcgaggcag cggatgccgt tgccgctcat
     2161 ctcggccggt ccgccatcgg cgttgaagag cgtcatcgtg caatcgacct cgtggtcgcc
     2221 gggtcccagg accatgagcc cgtcggcgcc gacccctcga cgccggt
//
LOCUS       DATCCH010000259         2078 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_526570, whole genome shotgun sequence.
ACCESSION   DATCCH010000259 DATCCH010000000
VERSION     DATCCH010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526570"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..1878)
                     /locus_tag="VIJ44_05055"
     CDS             complement(118..1878)
                     /locus_tag="VIJ44_05055"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY5165301.1"
                     /translation="MLSDYASYENGIGLDWYALDPNLRALLDRLLPDPDDRAFAEQHV
                     AEYGVLCGGPLARRAEVTDKHGPVLRRYDRWGQEVDVVEHHPTWLESKADLVRAGFTG
                     LPYHAGRPVPAVVTASLAYLVSQAETAIYCGLGMTAGAADIVERFAPPAVRDEMLSRL
                     TSLDPDEAWEGGMFLTERQGGSDVGANTTRAVPDGGEWRLHGEKYFCSNVDAEVFIVL
                     ARPDGAPEGARGLATFIVPRLLPDGSANGFRIKRLKPKLGTTGVPTGEVILEGARAWL
                     ASPMPRESDPAPSTGPVRGLNTMMEMVNGSRFGVALMGLGIHRRSFLEAAIYASRRQQ
                     WGQRIDRYPLVRETLVDLVVDLEAGAALTFECAVATRGADDEDAGRRLRRILVPLAKV
                     RATRAAIGAASQAVEMLGGNGYMEDWPMARQLRDAQCHTIWEGTENICCLDVRRAMRT
                     DGAHDVLLERVERALAGADPSPVLAPAIDAVAATLGDARTAIAHLETVSNELQLLNTR
                     RLTYLLSDLVQGALLLDEASWALRRDDARKAVVARRFAGQHLVTARARGILEDDRTVL
                     DLFEPLTRYGAIEVAAAVAA"
BASE COUNT          299 a          742 c          746 g          291 t
ORIGIN      
        1 cggtcaacca ggtttgagtc ccgttgaggt agctgtcgac cggcagaccg aaccggtcgt
       61 ccaacgccac gacgagggcc gccgacaccg gccacaccgc cgccgggacc cggtcgctca
      121 cgccgcgacg gccgcggcga cctcgatcgc cccgtagcga gtgagcggct cgaacagatc
      181 cagcacggtg cggtcgtcct cgaggatgcc ccgagcgcgg gccgtcacca ggtgctgacc
      241 ggcgaagcgt cgggcgacga ccgccttgcg ggcgtcatcg cgccgtaatg cccaactcgc
      301 ctcgtcgagg agcaacgcgc cctgcacgag atccgacagg agataggtca agcggcgggt
      361 gttcaacagc tgcagctcgt tcgacaccgt ctcgagatgc gcgatcgcgg ttcgggcgtc
      421 gccaagggtc gccgcgaccg cgtcgatcgc gggagccagc accggtgacg ggtccgcgcc
      481 ggcgagggca cgctcgaccc gctcgagcag cacgtcgtgg gctccgtcgg tgcgcatggc
      541 ccggcgcacg tcgaggcagc agatgttctc ggtgccctcc cagatggtgt ggcactgggc
      601 gtcgcgtagc tggcgggcca tcggccagtc ctccatgtac ccgttcccgc cgagcatctc
      661 gacggcctgg ctggccgcgc cgatcgcggc gcgggtggcc cgcaccttcg ccagcggcac
      721 gaggatccgg cgcagacggc gcccggcgtc ttcgtcgtcg gcgccgcgcg tcgcgaccgc
      781 gcactcgaac gtcaacgccg cgcccgcctc gaggtcgacg accagatcca cgagcgtctc
      841 gcggaccagc ggatagcggt cgatgcgctg accccactgc tggcggcggg acgcgtagat
      901 cgcagcttcg aggaacgacc ggcgatggat ccccaatccc atgagggcca cgccgaaccg
      961 gctgccgttg accatctcca tcatggtgtt gaggccccgg accggtcctg tgctcggcgc
     1021 ggggtcggac tcgcgcggca tcgggctcgc cagccacgcc cgtgccccct cgaggatcac
     1081 ctcgccggtc gggacaccgg tggtgccgag cttcggcttg agccgcttga tccgaaagcc
     1141 gttcgccgac ccgtccggaa ggagccgcgg gacgatgaac gtggcgagcc ctcgggcccc
     1201 ttcgggggcg ccgtcgggtc gggcgaggac gatgaacacc tcggcgtcga cgttcgagca
     1261 gaagtacttc tcgccgtgga ggcgccattc gccaccgtcg ggcacggcgc gggtggtgtt
     1321 cgcccccacg tcgctgcccc cctgacgttc ggtgaggaac atgccgcctt cccacgcctc
     1381 gtcagggtcg aggctcgtca ggcgactcag catctcgtca cgcaccgccg ggggcgcgaa
     1441 gcgctcgacg atgtccgccg cgccggcggt catgccgagc ccgcagtaga tggcggtctc
     1501 ggcctggctg accaggtagg cgagcgacgc ggtgacgacc gccggcaccg gtcgccccgc
     1561 gtggtacggg agcccggtga agcccgcgcg taccagatcg gccttgctct ccagccacgt
     1621 cgggtgatgc tcgacgacgt cgacctcctg gccccaccgg tcgtatcggc gcagcaccgg
     1681 gccgtgcttg tcggtcacct cggcgcgccg ggccagcgga ccgccgcaca gcacgccgta
     1741 ctcggcgacg tgctgctcgg cgaaggcccg atcgtcggga tccgggagca gccgatccag
     1801 cagtgcccgc aggttcgggt cgagggcgta ccagtcgagg ccgatgccgt tctcgtagct
     1861 cgcgtagtcg gacagcacga gtcgagggta ccgtgcccgc atctggcgag gacgagagcg
     1921 acaacccttg cggcgacgcg ccgccgcgct tctggtcgtg gcggggctcg cggcctgcgg
     1981 cggcggtggg ggtgggccca gcggaccggt gttgagccgc gccggcctgg tgagtcgggt
     2041 gaacgccgaa tgccgcaagc tcatgacggc aagcaacg
//
LOCUS       DATCCH010000260         2172 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_13757, whole genome shotgun sequence.
ACCESSION   DATCCH010000260 DATCCH010000000
VERSION     DATCCH010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13757"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1522)
                     /locus_tag="VIJ44_05060"
     CDS             complement(<1..1522)
                     /locus_tag="VIJ44_05060"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY5165302.1"
                     /translation="MSDSLGAAAAAVDDAAAVVDAAWVRLAHAAGGGSGRVGGAALDD
                     HQVLAYDLAHAASAVEAARVMLVYAEHGEHESRLARFFIADAVADLAAKLTGREALWG
                     VEAAELTACQPFLEAHRSPGFVDEIAEHLVRDGTGGAHLDDEFELVRDTFHRFALDRI
                     RPVAERVHRENIDVPEDIVGGLAELGGFGLSVPEEYGGFAVGGPHDYLGMVVATEELS
                     WGSLGVGGSLITRPEILTRALVKGGTEEQKQRWLPRIATGELMVGVMVTEPDYGSDVA
                     SLKTSATPVDGGYRINGVKTWATFAGRADILMLLARTDPDRSAGHRGLSLFVVDKEPA
                     PGHAFAFTQADATPGGGGKMEGRAIDTIGYRGMHSYEVAFDDWFVPAENLVGGPDGLG
                     KGFYLQMEGFENGRLQTAARAVGLMQAAFEAGLAYAQDRPVFGKAVFDYQLSRAKLVR
                     MAALIQAGRQFSYDVAGQMGRGEGTLGASMVKAYVCRAAEWVTREAMQLHGGMGYAE"
     gene            complement(1534..>2172)
                     /locus_tag="VIJ44_05065"
     CDS             complement(1534..>2172)
                     /locus_tag="VIJ44_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165303.1"
                     /translation="RLDPEVVVFIIDTNDSAIVSSAGTAGAASDWKARYAELVTQMLD
                     ILGGPDSHRYVYWVGGPTIRDTTIDSGVRQLDDVARTVVEHRPHATYVDDYHLFSDSQ
                     GRFSFTLPDPAGKPVLVRTDDGVHFTPAGADRLARPIFESLDTLCQLHAHAVPGSPKA
                     VIETPGSNQVGSNRGSPSTTRPTPPPVTSPPPTAPPTTPPPVVTSSLPTSRT"
BASE COUNT          325 a          753 c          771 g          323 t
ORIGIN      
        1 cctcggcgta ccccatgccg ccatggagct gcatcgcctc ccgggtcacc cactccgcgg
       61 cgcgacacac gtacgccttg accatcgacg cgcccagggt cccctcaccg cgacccatct
      121 gtcccgccac gtcataggag aactggcgac cggcttggat gagcgcagcc atgcggacca
      181 gcttcgcccg gctcagctgg tagtcgaaca ccgctttgcc gaacaccggt cggtcctgcg
      241 cgtacgcgag gccggcttcg aacgccgctt gcatcagccc gacggcacgg gctgcggtct
      301 gcagccgccc gttctcgaac ccttccatct ggaggtagaa ccccttgccg agcccgtcgg
      361 gcccaccgac gaggttctcg gcgggcacga accagtcgtc gaacgccacc tcataggagt
      421 gcatgccgcg gtagccgatc gtgtcgatgg ctcgcccttc catcttcccg ccgcctccgg
      481 gcgtggcgtc cgcttgcgta aacgcgaacg cgtgaccagg cgccggttcc ttgtcgacga
      541 cgaacaagga caggccccgg tggccggctg aacggtcggg gtcggtgcgg gcaaggagca
      601 tgaggatgtc agcgcgcccc gcgaacgtcg cccaggtctt gacgccgttg atccggtagc
      661 cgccgtcgac cggggtggcg ctggtcttga gactcgcgac gtcggacccg tagtcgggtt
      721 cggtgaccat gacgccgacc atcagctcgc cggtggcgat ccggggcagc caacgctgct
      781 tctgctcctc ggtgccgccc ttgaccagcg cgcgggtcag gatctcgggc cgggtgatca
      841 gggagccacc gacaccgagt gaaccccacg acagctcctc ggtggcgacg accatcccga
      901 ggtagtcgtg cgggcctccg accgcgaagc ctccgtactc ctccggcacc gacaggccga
      961 acccaccgag ctcggcgagc ccgccgacga tgtcctcggg gacgtcgatg ttctcgcggt
     1021 ggacacgctc agccaccggg cggatccggt cgagcgcgaa ccggtggaac gtgtcacgga
     1081 cgagttcgaa ctcgtcgtcg agatgcgcgc cgccggtccc gtcccggacg aggtgctcgg
     1141 cgatctcgtc gacgaacccg ggggagcggt gggcctcgag aaacggctgg caggcggtga
     1201 gctcggcggc ttcgacaccc cagagcgcct cgcggccggt gagcttcgcc gcgaggtccg
     1261 cgacggcatc ggcgatgaag aagcgggcca gccgagactc gtgctcaccg tgctcggcgt
     1321 agaccagcat cacccgggct gcttcgaccg cgctggccgc gtgggccagg tcgtaggcca
     1381 ggacctgatg atcgtcgagg gccgcgccac cgacccggcc gctcccaccc ccggccgcgt
     1441 gggcgaggcg cacccaggcg gcgtcgacga ccgcggcggc gtcgtcgacc gcggcggccg
     1501 cggcacctag cgagtcggac acggtgggtg agcctacgtg cgcgacgttg gcagcgacga
     1561 cgtcaccact ggtggtggcg tcgtcggggg cgcggtcggc ggcggtgacg tcaccggggg
     1621 cggggtgggc cgggtcgtcg acggcgatcc gcggttcgag ccgacctggt tgctgcctgg
     1681 agtctcgatc accgccttcg gtgatcctgg gaccgcgtgg gcgtgcaact gacacagcgt
     1741 gtcgagggac tcgaagattg gccgggccag ccggtcagcg ccggcgggag tgaagtggac
     1801 cccgtcgtcg gtacggacca gcaccggctt gccggccggg tcgggcaacg tgaagctgaa
     1861 ccggccctga ctgtcgctga agaggtggta gtcgtcgacg tacgtcgcat gcggacggtg
     1921 ctccacgacg gtgcgggcca cgtcgtcgag ctgacgcacc cccgagtcga tggtcgtgtc
     1981 ccgaatggtt gggccgccaa cccagtacac gtaacggtgc gagtccgggc cgcccaggat
     2041 gtcgagcatc tgggtgacga gctctgcgta gcgggccttc cagtccgacg ccgcgcccgc
     2101 cgtccccgcg gaggagacga tcgctgagtc gttggtgtcg atgatgaaca cgaccacctc
     2161 ggggtcgagg cg
//
LOCUS       DATCCH010000261         2115 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_429124, whole genome shotgun sequence.
ACCESSION   DATCCH010000261 DATCCH010000000
VERSION     DATCCH010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429124"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="VIJ44_05070"
     CDS             complement(<1..358)
                     /locus_tag="VIJ44_05070"
                     /inference="COORDINATES: protein motif:HMM:NF025279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin-like family protein"
                     /protein_id="HEY5165304.1"
                     /translation="MRDRYDEIQARGAEVVAVGTGDRRYAAAFSADEHVPFPVLVDDH
                     ADAATAASVRRVSFLRLMTDRRSWPGTRRARAGGHRIHRSGKRVTQLGATFVIGPGAV
                     LRYAHIDDHSADHAPLD"
     gene            552..1115
                     /locus_tag="VIJ44_05075"
     CDS             552..1115
                     /locus_tag="VIJ44_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY5165305.1"
                     /translation="MTTTDAPTTFTRMDESTAEQWAVIGKETMGHQDRVADRVLGMLR
                     SLSELTDGFAVDQLTHSLQTATRAERAGADDEVVVASLCHDIGKAVSVPNHPLIAAEI
                     LKSYVRDDTYQMIRAHQDFQGRHYYHHFGGDPDAREQYRGQPWFGLAEQFADEWDQVA
                     FDPGYDTLPLEHFEARVREVFGHPHSL"
     gene            complement(1126..>2115)
                     /locus_tag="VIJ44_05080"
     CDS             complement(1126..>2115)
                     /locus_tag="VIJ44_05080"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5165306.1"
                     /translation="DADDVYWGTRLACLEMIRTGTTRFWDMYWQPAAVARAVTDAGLR
                     ATVGLPLIDGFDPARGATLRADALRFLDELTDAGPHVTLSLTPHSIYAVSTESLTWVA
                     EQSATRDLPVHLHFLEIEDEVAGCIERTGNRPGVYLHDVGLLTPRTVLAHGVWMDPAD
                     LDLVAAGGATVVTNPVSNLKLAVGRIFDYPAARRRGIPTGLGTDGASSNNSLDLCSDL
                     KVFSILQKHAANDTTAVPADEAWRVATGARAPFLGGTPLAVGAPADFILVRLDAPELG
                     PGHLLANLVYAASGHVVDTTVVDGAILMRDGVIDGDEEIRAKARERAVRLGVL"
BASE COUNT          316 a          672 c          827 g          300 t
ORIGIN      
        1 cgtcgagtgg cgcatggtcg gcggagtggt cgtcgatgtg cgcgtagcgg agcacggcgc
       61 ccggtccgat cacgaatgtc gcgccgagct gcgtgactcg cttcccggaa cggtggatgc
      121 ggtgcccgcc agcgcgggct cgacgggtgc cgggccacga gcggcggtcc gtcatgaggc
      181 ggaggaacga cacccgtcgc accgacgcgg cggtggcggc gtcggcgtgg tcgtcgacga
      241 ggaccgggaa cgggacgtgc tcgtcggcgc tgaacgccgc ggcgtaccgg cggtcaccgg
      301 tcccgacggc gacaacctcc gctcctcggg cttggatctc gtcgtagcgg tcgcgcaact
      361 gcgccgcgtg ctcccgacag agcagtcagc cgaagtggcg gaggaagacc aggatgaccg
      421 cccggtcggc ccagtaggcc ccgagctggt ggggggcgcc gtcggcgtcg gggagcgtga
      481 cggcgctgag ctcgtcgagg tccacggctc ggacggtagt cggcgaggct gcgacgtggg
      541 agaatggcgt catgacgacc acggacgcac cgacgacctt cacccggatg gacgagtcca
      601 ccgccgagca gtgggcggtg atcggcaagg agacgatggg gcaccaggac cgcgtcgccg
      661 accgggtcct cgggatgctg cggtcgctgt cggagctcac cgacgggttc gcggtcgacc
      721 agctcaccca ctcgttgcag accgcgacgc gcgccgagcg ggccggggcc gatgatgagg
      781 tcgtggtggc ttcgctctgc cacgacatcg gcaaggcggt cagcgtgccc aaccatccgc
      841 tgatcgcggc cgagatcctg aagtcgtacg tgcgcgacga cacctaccag atgatccggg
      901 ctcaccagga cttccagggt cggcactact accaccattt cggtggcgat cccgacgcgc
      961 gtgagcagta ccggggtcag ccgtggttcg ggttggccga gcagttcgcc gacgagtggg
     1021 atcaggtggc gttcgacccc gggtacgaca cgctccccct cgagcacttc gaggcgcggg
     1081 tgcgcgaggt gttcgggcac ccgcactcgc tgtagaccga gccgctcaca gcaccccgag
     1141 ccgcacggcg cgttcgcggg ctttggcgcg gatctcctcg tcgccgtcga tgacgccgtc
     1201 gcgcatgaga atcgcgccgt ccacgaccgt ggtgtcgaca acgtggccgg acgcggcgta
     1261 gacgaggttg gccagcaggt ggccggggcc gagttcgggg gcgtcgaggc ggaccaggat
     1321 gaagtccgcg ggggcaccga cggccaatgg ggtgccacca aggaacgggg ctcgggcgcc
     1381 cgtcgcgacg cgccacgcct cgtcggccgg gaccgcggtg gtgtcgttgg cggcgtgctt
     1441 ctggaggatc gagaagacct tgaggtccga gcagaggtcg aggctgttgt tcgacgaggc
     1501 gccgtcagtg ccgagcccgg tcgggatacc gcgccgtcgg gccgccggat agtcaaagat
     1561 ccgtcccacc gcgagcttga ggttcgacac cgggttcgtc acgacggtgg cgcctccggc
     1621 ggcgacgagg tcgaggtcgg cggggtccat ccacacgccg tgggccagga ccgtccgggg
     1681 ggtgaggagc ccgacgtcgt ggaggtagac accgggccgg ttcccagtcc gttcgatgca
     1741 gccggcgacc tcgtcctcga tctcgaggaa gtggaggtgg acgggcaggt cgcgggtggc
     1801 ggactgctcg gcgacccagg tcaacgattc ggtgctgacg gcgtagatgc tgtgcggggt
     1861 gagagacaag gtgacgtgcg gtccggcgtc ggtgagctcg tcgaggaacc gcaacgcgtc
     1921 ggcccggagg gtcgcgccgc gggccgggtc gaacccgtcg atgaggggga ggccgacggt
     1981 ggctcggagc ccggcgtcgg tgacggcgcg ggccaccgcg gcgggctgcc agtacatgtc
     2041 ccagaaccgg gtggtgccgg tgcggatcat ctcgaggcac gccaggcgcg tcccccagta
     2101 gacgtcgtcg gcatc
//
LOCUS       DATCCH010000262         3666 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_138573, whole genome shotgun sequence.
ACCESSION   DATCCH010000262 DATCCH010000000
VERSION     DATCCH010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3666
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138573"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VIJ44_05085"
     CDS             complement(<1..236)
                     /locus_tag="VIJ44_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165307.1"
                     /translation="MGRVDELVEATGGVVELVSLRTAWSAAAIVSDRFPGRDNPAWGA
                     TLRPAVNWFRLTFLNRAIINRDRTGVIGVGGTRLG"
     gene            complement(342..1658)
                     /locus_tag="VIJ44_05090"
     CDS             complement(342..1658)
                     /locus_tag="VIJ44_05090"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEY5165308.1"
                     /translation="MSSETEPTFSRALSAWLTAPFLVAVAVAVPLALNGEHPALGSLL
                     LLAALCTFAEHVIVRLPNGSSVSASVMISLAAVFVFRDNAPLLGPLLVGMAGGIYWDH
                     LRRRDWRRLAFNIGTLGLSTLAAALALTGGTHAGVLLVLLGAVLAAVAYWLVDIVLLS
                     VAIRLRDGGSLRSQFTELWGFADFEIVIFAVLGVFLGKLYQVHGVLIVVLFITPILAA
                     RQAFASYLAANEQHEATLAVLVHALEAKDPYTGGHVARVAQFSTYIAAELGFSPSRLR
                     RLHDAALMHDIGKLTVPNRLLNKPGRLTPSEYEQVRRHEPISVQLVRQIEALAPCAAA
                     IDGEGLDAPLESRIIHVADAFDAMTSSRAYRRAMAQEDAVAELQSHAGQQFDADCVGA
                     LLRVLERRGERYGAGVEAVAAIAHFKVPPPVVGVGSAGLGDLAPES"
     gene            complement(2128..3096)
                     /locus_tag="VIJ44_05095"
     CDS             complement(2128..3096)
                     /locus_tag="VIJ44_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020171962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEY5165309.1"
                     /translation="MPAVTVDDVLTLPAITPPNMTTTTPRPVVSVTNAPSGHEGLGFP
                     VRRAFAGVDARLLDPFVHMDQMGEVDYGPGEPKGTPWHPHRGFETVTYMIDGTFQHQD
                     STGGGGLITDGATQWMTAGAGILHIETPPQELVTSGGVFHGIQLWVNLPAAQKWVAPN
                     YQDIPAERVTLLRSADGGALVRLIAGNVGGHSGPGSTHTPIAVAHATLSASAELTLPW
                     APAFNALVYALHGRGTVGPDRRPFGTGQLAVFGPGDTITVAADTSAGEGGSSLDVLIL
                     GGQPIREPVAWYGPFVMNTESEIAQALEDFEAGRMGTVPAGALGTR"
     gene            complement(3246..>3666)
                     /locus_tag="VIJ44_05100"
     CDS             complement(3246..>3666)
                     /locus_tag="VIJ44_05100"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY5165310.1"
                     /translation="LIGALTPDEVQADLTEAVQRLRDLGAAKVGITGFCLGGTISYRA
                     ALSGLDLACAAPFYGGHIPRELGRPACSILAFFGGRDEYIPEAAVEAVRAHHPDDVVV
                     YPDAEHGFMRDGSPSYHPAAAVDAWSRLLTFFDQHLR"
BASE COUNT          576 a         1171 c         1369 g          550 t
ORIGIN      
        1 ccgagccggg tcccgccgac gccgatgacg ccggtgcggt cccgattgat gatcgcccga
       61 ttgaggaagg tcaaccggaa ccagttgacg gctggtcgta gcgtggcgcc ccaggcgggg
      121 ttgtctcgcc ccggaaaccg atccgacacg atcgccgcgg ccgaccaggc ggtgcgcaga
      181 ctgacaagct cgacgacccc gcccgtcgct tcgacgagct catcgacacg acccacgatg
      241 cgagtctcgc acgtcgggtg tccgatccgg ttccaatgcg cggcgacgcc gtggcggtcg
      301 ccgaggcgcg gtcaggtcgt cgcgtcgccg ggtgggagcg gtcaggactc gggggcgagg
      361 tcgccgagac cggcggaccc gacgccgacg acggggggcg ggaccttgaa gtgcgcgatc
      421 gccgcgacgg cttcgacgcc ggcgccgtac cgctccccgc ggcgttcgag tactcgcagc
      481 agggccccga cacagtcggc gtcgaattgc tgtccggcgt ggctttggag ctcggcgacg
      541 gcgtcttcct gggccattgc ccggcgatag gcgcggctcg aggtcatggc gtcgaacgcg
      601 tcggcgacgt ggatgatgcg gctctcgagt ggtgcgtcga ggccttcgcc gtcgatcgcg
      661 gccgcgcacg gcgcgagcgc ctcgatctgg cggacgagct ggacgctgat cggctcgtgt
      721 cgtcggacct gctcgtactc ggatggagtg aggcgcccgg gcttgttgag caggcgattc
      781 gggaccgtga gcttgccgat gtcgtgcatc agcgccgcgt cgtggaggcg gcggagccga
      841 cttggcgaga agcccagctc ggcggcgatg taggtggaga actgggcgac tcgcgcgacg
      901 tgcccgccgg tgtatgggtc cttcgcttcg agggcgtgca ccagcaccgc gagggttgcc
      961 tcgtgctgct cgttcgcggc gaggtacgag gcgaacgcct ggcgggcggc gaggattggg
     1021 gtgatgaaca gcacgacgat cagcaccccg tggacctggt agagcttgcc gaggaacacg
     1081 ccgagcaccg cgaagatgac gatctcgaag tcggcgaagc cccacagctc ggtgaactgt
     1141 gagcgcagcg atccgccgtc gcggaggcgg atggcgaccg agaggagcac gatgtcgacc
     1201 agccagtagg ccaccgccgc gaggaccgcg ccgaggagga ccaggaggac gccggcgtgg
     1261 gttccgccgg tcagcgcgag cgccgccgcg agcgtggaga gccccagggt gccgatgttg
     1321 aacgccagcc ggcgccagtc tcggcggcgg agatggtccc aatagatgcc gccggccatc
     1381 cctaccagca gcggtccgag gagcggtgcg ttgtcgcgga acacgaacac cgcggcgagc
     1441 gagatcatca ctgacgccga gaccgacgag ccgttcggga gtcggacgat gacatgctcg
     1501 gcgaacgtgc acagcgccgc gaggagcagc agcgacccga gcgcgggatg ctcaccgttg
     1561 agcgcgaggg gcaccgcgac cgcgaccgcg accaaaaacg gcgcggtcag ccacgcgctc
     1621 agcgcgcggg agaacgtcgg ctcggtctcg ctgctcatga tggtggtgtc ggcgctccga
     1681 gccgtggggg caagatcccg ggaagttagg tgtgggtcgg gattatttga aggtccggcc
     1741 ggctccaccg gcgagcacca ccgcagcgat actcgatccg attagagcga cgcgggcaac
     1801 tacacgcttg ctcacgtcac accccctcct tccgaaggag ctgatgccga cctgcgctag
     1861 gtggtcgcac cgctgcacgc ttcgctgtga agttgggggc ttgttagccc tcagttctct
     1921 cgacccggga ccctgccctc gcggcccggc ggacgctccc ttcgttacaa cagcaacaac
     1981 aagaacccca attgtcacgc aaccgacggc cgagggcaac cccgagaatt ggtctgacca
     2041 ctctgagcgc gaaaacctgg aggggcagtt ggggggctgt ccctcagggt gaggctcagc
     2101 ggccgagccg acgggcgacg gcggcgttca gcgcgttccg agggcaccgg ccgggacggt
     2161 gcccatgcga ccggcttcga aatcttcgag ggcttgggcg atctcggact cggtgttcat
     2221 cacgaacggt ccgtaccagg cgaccggctc acggatcggt tggccgccga ggatgaggac
     2281 gtcgaggctg gatcctccct cgcccgctga ggtgtcggcc gcgaccgtga tggtgtcgcc
     2341 gggcccgaac accgcgagct ggccggtccc gaacgggcgc cgatcgggtc cgacggtgcc
     2401 ccggccgtgg agcgcgtaga ccagcgcgtt gaacgccgga gcccacggca gggtcagctc
     2461 ggcgctcgcg ctcagggttg cgtgcgcgac ggcgatgggc gtgtgggtgg agccgggacc
     2521 cgagtggccc ccgacgttcc cggcgatgag tcgaacgagg gcgccgccgt cggcgctgcg
     2581 cagcagcgtg acgcgctcgg cggggatgtc ctggtagttg ggcgcgaccc acttctgggc
     2641 cgcgggcagg ttcacccaga gctggatccc gtggaacacg ccgccgctgg tgacgagctc
     2701 ttggggtggt gtctcgatat ggaggattcc ggctcctgcg gtcatccact gggtggctcc
     2761 gtcggtgatg agcccgccac caccggtgga gtcctgatgt tggaacgttc cgtcgatcat
     2821 gtaggtgacg gtctcgaatc cacggtgcgg atgccagggt gtccctttgg gttcgccggg
     2881 tccgtagtcg acctcgccca tctggtccat gtgcacgaac ggatcgagca ggcgggcgtc
     2941 gacaccggcg aacgcgcggc gcaccgggaa gccgagacct tcgtgacccg acggcgcgtt
     3001 ggtgaccgac accacggggc gcggtgtggt cgtcgtcatg ttcggtgggg tgatcgcagg
     3061 gagtgtcagc acgtcgtcga ccgtcaccgc gggcatggaa cctccttggt cttccggctg
     3121 gtgcggggtc gctccggtga gcgccgatcg cgaccacaag ccgaggttct ggctgtttca
     3181 acgtccgggc gaggcgattc catcccggga ccgtctgacg cgaccctcca cctggcggcc
     3241 gggggtcagc gcaggtgctg gtcgaagaag gtgaggagac gtgaccacgc gtcgacggcg
     3301 gccgcggggt gatagctcgg cgagccgtcc cgcatgaacc cgtgttccgc gtcggggtag
     3361 acgacgacgt cgtcgggatg gtgggcccgg accgcctcga cggcggcctc ggggatgtac
     3421 tcgtctcggc cgccgaagaa cgccaggatc gaacaggcgg gacggccgag ctcacggggg
     3481 atatgaccgc cgtagaacgg tgccgcacag gccaggtcca ggccggagag cgcggcgcga
     3541 tagctgatgg tcccgccgag acagaagccg gtgatcccca ccttcgccgc cccgaggtca
     3601 cggagccgct ggacggcttc ggtgaggtcg gcctgcactt cgtcgggcgt gagcgcaccg
     3661 atgagg
//
LOCUS       DATCCH010000263         2677 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_195241, whole genome shotgun sequence.
ACCESSION   DATCCH010000263 DATCCH010000000
VERSION     DATCCH010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195241"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /locus_tag="VIJ44_05105"
     CDS             complement(<1..856)
                     /locus_tag="VIJ44_05105"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY5165311.1"
                     /translation="MTRRVSGARLLAVGATAAFVVFVAVQFWNANQTVDANKIILFLV
                     VGVTLGSIYAVAAAGLVVTYTTSGIFNFAQGAIGMILTFVYWQLKVGWGLQTLLALGI
                     TVLIAAPLLGAAIERVFMRRLTQAPLVASLVATVGLLVFFVGLADTVWPSDTYRTIGT
                     FFGTNKIQIGGTFIPWFRIITIIVGIVIAVLLWLLLRRTRIGIAMRAVVDNRELASLN
                     GARPGRISMFSWALGSSMAAMAGIFLAEELGQLSSQGLTFFIVDAFGAAIIGRLRSLP
                     LTLVGGAVI"
     gene            complement(975..2309)
                     /locus_tag="VIJ44_05110"
     CDS             complement(975..2309)
                     /locus_tag="VIJ44_05110"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5165312.1"
                     /translation="MRRFRWLIAPVVVTGMALGMSGVAPAQTGGGSARTASETGITPT
                     TINITVPAAIDVPGSAGLFQGSITGVKAWADYENNVNHGLAGRKIVVNGIDTKLGSDL
                     ATNAFISACNSSFATIGTSVLLAQDFSELTSCADKTGAATGLPDFPVVVTEVAQQCSP
                     VSFPVNPPTLDCATKSQHPQTYRAGTGPASYFTKKFGKLKTTFLYPSPAESASAKAAQ
                     VPIFTAEQQKGITQIFTQDVSAFATRGEYTPIAAAIASNGSTYAQSGLDVAGTIKLRQ
                     EAATQQTNVKVWNCSLQCYDSKLIAQGGSAVEGEYVYTPFLPFLGKNNESKQNPMLAN
                     FLKYDKTPDGFGIQAFAAGLYFADLVNKIVAKGGPNALTRKAVIAAAPTVHNFNAGGM
                     IGTTDVGARTPSPCYVLNQVKNGDYVRVLPTAKGTFNCDASNVVSLKLDLIK"
     gene            complement(2435..>2677)
                     /locus_tag="VIJ44_05115"
     CDS             complement(2435..>2677)
                     /locus_tag="VIJ44_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007733359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase"
                     /protein_id="HEY5165313.1"
                     /translation="GIADLVRAPEDPSQFDTYHPANVSPVVAWLATEDCPMNGRVVYV
                     RGGELRVMEGWRQGPVLEHDGPRTVADCARALTDRA"
BASE COUNT          428 a          875 c          930 g          444 t
ORIGIN      
        1 cgatgaccgc gcccccgacg agcgtcagcg ggaggctgcg gagccgaccg atgatcgccg
       61 cgccgaacgc atcgacgatg aagaacgtca agccctgtga gctcagctgt ccgagttctt
      121 cggcgaggaa gatgcccgcc atggcggcca tggacgagcc cagcgcccac gagaacatcg
      181 agattcgccc cggccgcgct ccgttcaacg aggcgagctc gcggttgtcg accacggcgc
      241 gcatcgcgat cccgatccga gtccgacgga gcaggagcca gagcagcacc gcgatgacga
      301 tgccgacgat gatcgtgatg atccggaacc aggggatgaa cgtccccccg atctggatct
      361 tgttcgtccc gaagaaggtc ccgatcgtgc ggtacgtgtc ggatggccac acggtgtcgg
      421 ccagcccgac gaagaacacg aggagaccga ccgtcgcgac gagcgaggcg acgagcggcg
      481 cctgcgtcag gcgtcgcatg aacacccgct cgatcgccgc gccgagcagc ggggcagcga
      541 tgaggacggt gatcccgagg gccagcaggg tctggaggcc ccacccgacc ttcagctgcc
      601 agtacacgaa ggtgaggatc atcccgatcg cgccctgcgc gaagttgaag attcccgacg
      661 tcgtgtacgt gaccacgagc ccggccgccg ccactgcgta gatcgacccg agcgtcaccc
      721 caaccacgag aaacaggatg atcttgttcg cgtcgacggt ctggttcgcg ttccagaatt
      781 gcaccgcgac gaacacgacg aacgcggcgg tcgcgccgac ggccaggagc ctcgcgccgc
      841 tcactcgcct ggtcatccgt tcgacggtgg ccgccacccg gtcgtcgcct cctggcgggg
      901 gtcacacacc cgaagcggga cgggccgccc ggcccaatgg tcgggcggcc cgctcttgcg
      961 cttgaggtac ggccctactt gattaggtcg agtttcagcg acacgacgtt gctcgcatcg
     1021 cagttgaacg tgcctttggc cgtcggcagc acccggacgt agtcgccatt cttcacctgg
     1081 ttcaggacgt agcaggggct gggtgtccgt gccccgacgt cggtggtgcc gatcatccca
     1141 ccggcgttga agttgtggac cgtcggtgct gcggcgatca ccgccttgcg ggtcagcgcg
     1201 ttcgggccac ccttcgccac gatcttgttc accaggtcgg cgaagtagag gccggcggcg
     1261 aacgcctgaa tgccgaagcc gtctggggtc ttgtcgtact tgaggaagtt ggcgagcatc
     1321 gggttctgct tgctctcgtt gttcttcccg aggaacggca ggaacggcgt gtagacgtat
     1381 tcgccctcga cggcggagcc gccctgagcg atcagcttgg agtcgtagca ctggagggag
     1441 cagttccaga ccttgacgtt ggtctgctgg gtcgcggcct cctggcggag cttgatggtg
     1501 cccgcgacgt cgaggccact ctgggcgtac gtggagccgt tgctcgcgat cgcggccgcg
     1561 atcggggtgt actcgcctcg ggtcgcgaac gccgacacgt cctgcgtgaa gatctgtgtg
     1621 atgcccttct gctgctcggc ggtgaagatc ggcacctgcg ccgccttcgc cgacgcgctc
     1681 tcggccgggg atgggtacag gaacgtggtc ttgagcttgc cgaacttctt cgtgaagtac
     1741 gacgccggac cggtgcccgc gcggtaggtc tgggggtgct ggctcttcgt ggcgcagtcc
     1801 agcgtgggcg ggttgaccgg gaacgaaacc ggcgagcact gctgggcgac ctcggtcacg
     1861 acgaccggga agtcgggcag gcccgtcgcc gccccggtct tgtccgcgca ggaggtgagc
     1921 tcactgaagt cctgggcgag cagcaccgag gtcccgatcg tggcgaagct gctgttgcac
     1981 gccgagatga aggcgttggt ggcgagatcc gagcccagct tcgtgtcgat gccgttcacg
     2041 acgatcttgc gtccggccag cccgtggttg acgttgttct cgtagtccgc ccacgccttg
     2101 accccggtaa tcgagccttg gaagaggccc gcggatcccg ggacgtcgat cgcggccggg
     2161 accgtgatgt tgatggtggt cggggtgatg cccgtctccg acgcggtgcg cgccgagcca
     2221 ccaccggttt gggcgggcgc tacaccgctc attcccaggg ccatgcccgt caccacgacc
     2281 ggcgcgatca gccagcggaa tcgcctcacg gtacccccct tgtttgccgg ccccccatag
     2341 gcaccgaccg tgtgaaactg cgcggaaatc taactgacgc atcgtcaggt gtccacgcgg
     2401 ggggcgagca gcaaccacgc ggcggcgccg acggtcacgc tcggtcggtg agggcgcggg
     2461 cgcagtcggc gacggtgcgg gggccgtcgt gctcgaggac gggcccctgg cgccaccctt
     2521 ccatgacccg gagctcgccg ccgcggacgt acacgacccg gccgttcatc gggcagtcct
     2581 cggtggccag ccacgccacc accggagaca cattcgcggg gtggtaggtg tcgaactggc
     2641 tcgggtcctc gggggctcgg acgaggtcgg cgatgcc
//
LOCUS       DATCCH010000264         5390 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_278006, whole genome shotgun sequence.
ACCESSION   DATCCH010000264 DATCCH010000000
VERSION     DATCCH010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5390
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278006"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="VIJ44_05120"
     CDS             complement(<1..693)
                     /locus_tag="VIJ44_05120"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY5165314.1"
                     /translation="MLAGAAAPQLRDRIRRAGPITFAEYQGVALDAFFARGHGAGRAG
                     RDFVTSPEVGTLFGALIARGLDETWRGLGAPDPFVVVDAGGGRGRLLADVLRAQPDCS
                     PALRGVLVERSPALRAEARTLLPLEPADVVLGPFAPGDPDSAAEPIPGSGPVVTALDE
                     LPAASFTGVIVANELLDNLPVRIVERTERGWMELRVGLAPDEGHFAEVVVAAPRDLAR
                     LADGLTAETPVRP"
     gene            complement(734..2191)
                     /locus_tag="VIJ44_05125"
     CDS             complement(734..2191)
                     /locus_tag="VIJ44_05125"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEY5165315.1"
                     /translation="MNPHVDFHALAPEIVLTATIILVLIVDLFTDDRRLPAQLAGFGV
                     LGSLVPVLTLAVDGTRRSLFGGAFIVDNYSLVLTGFFLVAGYVSLLVSVDYIGEGDYY
                     QGEYYVLLLTSLLGMVAMASARDLVSIFVALETITIPTFVLAGWRKHDTRSNEASLKY
                     FLIGALSSAVMLYGMSIVFGLANSTVLVDINKYAAGATPALFAVAVFLTLVGFAFKIS
                     AVPFHFWAPDTYEGAPTPVTAFLSVASKAGGFVALLTVIRFGFFSSGDSWQPVLWVLA
                     AASMTLGNFAALRQTNIVRMLAYSSVAQGGFILVPLAVAGDVHGNSAWQAVVIYILIY
                     GAMNLGAFAAVIAVARRTRSAEISDYSGLGRTSPGIAVVFTLFLFSLAGVPPLAGWFA
                     KFVMFRAVFEANTTAAVVLGVIAAVNSVVAFFYYAGVARRFWFEEPPGVAAPAPARLP
                     IALTGALGLMATVVLVVGVYPQFFARLGELAFQRG"
     gene            complement(2195..3739)
                     /locus_tag="VIJ44_05130"
     CDS             complement(2195..3739)
                     /locus_tag="VIJ44_05130"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEY5165316.1"
                     /translation="MHWFDDWALTLAVFVPLVGLAVVLFIPRAHEQAIKTTALVASLV
                     TAAVGIGILTDFDYAHGGTLQFNVNKSWIPVIKSRYHMGLDGIALPLLILSMLMVVLC
                     IIYSWDHFPEPHNPKGFLALILLLEIGMNGTFVAQDLILFFVFFELVLLPMFFMIGVW
                     GGPNRQYAAIKFFLFTLFGSALMLVSFLAIFFLSKTQTFDIPKLAQLGGAGIAHGTEV
                     LVFVGLFLGFAIKVPMFPFHTWLPDAHTEAPTVGSVLLAAILLKLGAYGFIRIALPIL
                     PGAAQTWAPWIGLLAVIGIIYGALCCLAQRDMKRLIAFSSVSHMGFVMLGIATLTSFG
                     LNAAIFGMVAHGLITGMLFFLAGSVQERYGTREMSRLGGLLTQVPKLGWILGFCVMAS
                     LGLPGLAGFWGEFPAILSAYDPAKLAQGLLNGDHLLTFRVFMVIAAIGTVLAAGYLLW
                     MFQRVAFGTVKAEFEHAHIHDLHIPEWIAWVPLLLLITALGIYPNLVFHITNGAVSNV
                     TKVFGG"
     gene            complement(3758..>5390)
                     /locus_tag="VIJ44_05135"
     CDS             complement(3758..>5390)
                     /locus_tag="VIJ44_05135"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEY5165317.1"
                     /translation="ALSLFTASMLTLVVAENLIQLLVGWELVGVCSFMLIGHWWEEPE
                     NSRAAIKAFLTTRIGDIGLMIGIIILFFTVGSFGIGATNLYASGAHAHHDLLLAAAIC
                     LMIGIMGKSGQFPLHTWLPDAMAGPTPVSALIHAATMVVAGVFLGARVYPVFFNGFSI
                     GHGGVNLMALVGGVTIVIGAVLAFVQTDIKKVLAYSTISQLGYMVMALGVGAWTAAVF
                     HLFTHAFFKALLFLGAGSVSHGAGHTFDMREMGGLRKRMPTTFWTFVIGSAALAGIFP
                     LAGFWSKDEILVNAGANGFDAFLVVGLIGAFLTAAYMTRAVYLTFFGEYRGHEHPHES
                     ERLITVPLLILAGLSVVAGLINAAPLGIEKFKEWVDPQVAFPALSHAAFDYPKAVISV
                     AIAVLAIGIAAFYWLQHEEERAFKDLTKRNPLARAGYTFLVNKYYLDYLYEDVIIDGI
                     RGSVARASYWVNQHVIDGVVNGLGRGTSVLARYTYDVVDQRIVDGAVNGLAYEADAAS
                     GEFRRIQSGRVQRYALMLFLGVGVLSLAVFLTNVL"
BASE COUNT         1071 a         1640 c         1926 g          753 t
ORIGIN      
        1 gggcctcacc ggcgtttcgg cggtgaggcc gtcggcgaga cgggcgaggt ctcgcggcgc
       61 ggcgacaaca acttcggcga agtgaccctc gtccggggcg aggccgacgc gcagctccat
      121 ccatccccgc tcggttcgtt cgacgatccg caccgggagg ttgtcgagca gctcgttagc
      181 gacgatgacc ccggtgaacg aggctgcggg gagctcgtcg agcgcggtga cgacgggccc
      241 ggaaccgggg atgggctcgg cagccgagtc gggatctccg ggggcgaacg gtccgagtac
      301 gacgtcggcg ggttcgagcg ggaggagggt ccgggcctcg gctcgcaggg ccggcgagcg
      361 ttcgaccagg acgccccgca acgcggggct gcaatcgggc tgggcccgga ggacgtcggc
      421 gagcagccgc ccccggcctc cgccggcgtc gacgacgaca aacgggtcgg gggcaccgag
      481 gccgcgccag gtctcgtcga gtcctcgggc gatgagcgcg ccgaagaggg tgccaacttc
      541 ggggctggtg acgaaatcgc gtccggcgcg tcccgcgccg tgaccgcggg cgaagaaggc
      601 gtcgagggcg acgccttggt actcggcgaa cgtgatgggg cccgctcgtc gaattcggtc
      661 ccgtagctgc ggggcggcgg cgccagccag cacgggggtg gctaccgtcc gggcgcgcgc
      721 atcccagacc gggtcatccc cgctggaacg cgagctcccc gagccgggcg aagaactgcg
      781 gatacacgcc gacgacgagc accaccgtgg ccatgaggcc cagcgccccg gtcaacgcga
      841 tcgggagccg tgctggggcc ggcgccgcga cacccggcgg ctcctcgaac cagaaccggc
      901 gggcgacgcc ggcgtaatag aagaaggcga cgaccgagtt gacggcggca atcacgccga
      961 gcacgaccgc tgcggtcgtg ttggcttcga acacggcccg gaacatgacg aacttcgcga
     1021 accacccggc gagcggtggg acaccggcga gagagaagag gaacagggtg aacaccaccg
     1081 cgatccccgg gctcgtgcgt cccagcccgg aatagtccga gatctcggcg gagcgggtcc
     1141 ggcgagcgac ggcgatcact gcggcgaagg caccaaggtt catcgcgccg tagatgagga
     1201 tgtagatcac cacggcctgc caggccgagt tgccatggac atcgccggcc accgcgagcg
     1261 gcacgaggat gaagccgccc tgcgccactg acgagtaggc cagcatccgc acgatgttgg
     1321 tctggcgcag ggccgcgaag ttcccgagcg tcatggacgc cgccgccagc acccacagca
     1381 ccggttgcca cgagtcgccg gacgagaaga acccgaagcg gatgacggtg agcagcgcga
     1441 cgaaccctcc cgccttcgac gcgaccgaca ggaacgcggt gaccggggtc ggcgcccctt
     1501 cataggtgtc cggggcccag aaatggaacg ggaccgcgct gatcttgaac gcgaatccga
     1561 ccagggtgag gaagacggcg acggcgaaca gggccggcgt ggcacccgcg gcgtacttgt
     1621 tgatatcgac gaggacggtc gagttcgcga gcccgaagac gatcgacatc ccgtagagca
     1681 tcaccgccga cgacagcgca ccgatgagga agtatttgag actcgcctcg ttggagcggg
     1741 tgtcgtgctt gcgccagccg gccaggacga acgttgggat cgtgatggtc tcgagcgcca
     1801 cgaagatcga gacgaggtcc cgggccgacg ccatggcgac catgccgagc agcgaggtca
     1861 acaggaggac gtagtactca ccctggtagt agtcgccttc tccgatgtag tcgaccgaga
     1921 cgagcaggct gacgtagccg gcgacgagga agaagccagt caacaccagc gagtagttgt
     1981 cgacgatgaa cgccccgccg aagagggatc gccgggtccc gtccacggcc agggtcagca
     2041 ccgggaccag cgagcccagc actccgaacc ctgcaagctg ggcggggagt cgtcggtcgt
     2101 cggtgaagag gtccacgatc agcacgagga tgatcgtcgc ggtcaggacg atctccggcg
     2161 ccagcgcgtg gaagtcgacg tggggattca ccggtcagcc tccgaagacc ttcgtgacgt
     2221 tgctgaccgc gccattggtg atgtggaaca cgaggttcgg gtagatgccg agggccgtga
     2281 tgagcaacag caacgggacc cacgcgatcc attcggggat gtggaggtcg tgaatgtgcg
     2341 cgtgctcgaa ctcggccttg accgtcccga acgcgactcg ttggaacatc cacaggaggt
     2401 agccggcggc gaggaccgtt ccgatggcgg cgatgaccat gaacacccgg aaggtgagca
     2461 ggtgatcgcc gttcaggagc ccttgagcga gcttcgcggg gtcgtacgcg gacaggatcg
     2521 ccgggaactc gccccagaat cccgccaggc ccggcaaccc gagcgacgcc atgacgcaga
     2581 agcccaggat ccagccgagc ttgggcacct gggtgagcag gccaccgagc cggctcatct
     2641 cgcgggttcc gtagcgttcc tgaacggatc cggcgaggaa gaacagcatg ccggtgatga
     2701 ggccgtgggc caccatgccg aagatcgcgg cgttgagccc gaagctagtg agggtcgcga
     2761 tgccgagcat cacgaaaccc atgtgtgaga ccgacgagaa ggcgatcagg cgtttcatgt
     2821 cgcgctgcgc gagacagcac aaggcgccgt agatgatccc gatcacggcc agcagcccga
     2881 tccacggggc ccaggtctgg gccgcgccgg ggaggattgg caacgcgatc cggatgaagc
     2941 cgtaggcgcc gagcttcagg aggatcgcgg cgaggaggac cgagccgacc gtgggtgcct
     3001 cggtgtgggc gtcgggcaac caggtgtgga acgggaacat cggcaccttg atcgcgaagc
     3061 cgaggaagag tcccacgaag accagcacct cggtgccgtg ggcgatcccg gcccctccga
     3121 gctgggcgag cttcgggatg tcgaaggtct gggtcttcga caagaagaag atcgcgagga
     3181 agctcacgag catgagcgcg gagccgaaca acgtgaacaa gaagaacttg atggcggcgt
     3241 actggcggtt cggccctccc cagacgccga tcatgaagaa catggggagc aggacgagct
     3301 cgaagaagac gaagaacaag atgaggtcct gcgcgacgaa cgtcccgttc attccgatct
     3361 cgaggagcag gatgagcgcc aggaagccct tcgggttgtg cggctcgggg aagtggtccc
     3421 acgagtagat gatgcacagg acgaccatga gcatcgagag gatgaggagt gggagtgcga
     3481 ttccgtcgag acccatgtgg taacgggact tgatcaccgg gatccacgat ttgttgacgt
     3541 tgaactgcag cgttccgccg tgtgcgtagt cgaagtcggt gaggatcccg atgccgaccg
     3601 ccgcggtgac caacgacgcc acgagggccg tggtcttgat cgcctgctcg tgcgcgcggg
     3661 ggatgaacag caccaccgcc agcccgacca gcgggacgaa taccgccagg gtcaacgccc
     3721 agtcatcgaa ccagtgcatg gctgtcgata cctcgcttta gaggacgttc gttaggaaca
     3781 cggcgaggct taacacgccc acaccgagga agagcatgag tgcgtaccgc tgcacgcgcc
     3841 cggactggat tctgcggaat tcgccgctcg cagcatcagc ttcgtacgcg aggccattga
     3901 cggcaccgtc gacgatccgc tggtcgacga cgtcgtaggt gtagcgggcg aggacggatg
     3961 tgcctctccc gaggccgttg acgacgccat cgatgacgtg ctggttgacc caatacgagg
     4021 ctcgagccac cgagccgcgg atcccgtcga tgatgacgtc ttcgtagagg tagtccaggt
     4081 agtacttgtt gacgaggaac gtgtagcccg cccgggccag tgggttgcgc ttcgttaggt
     4141 ctttgaaggc gcgttcctct tcgtgctgga gccagtagaa ggcggcgatg ccgatggcga
     4201 ggacggcgat ggcgaccgag atcacggctt tcggatagtc gaaggcggcg tggctgagcg
     4261 ccgggaacgc gacctggggg tcgacccact ctttgaactt ctcgatgccg agtggcgcgg
     4321 cgttgatcag cccggcgacc accgagaggc cggccaggat caggagcggg acggtgatca
     4381 gacgctccga ttcgtgcggg tgctcgtgac ctcggtactc gccgaagaag gtgaggtaga
     4441 cggcgcgggt catgtaggcc gcggtgagaa acgcgccgat gagaccgacg acgaggaacg
     4501 cgtcgaatcc gttcgcgccg gcgttgacga ggatctcgtc tttggaccag aagccggcca
     4561 gcgggaagat cccggccagc gccgccgatc cgatcacgaa ggtccagaac gtggtcggca
     4621 tgcgcttgcg aaggccgccc atctcgcgca tgtcgaaggt gtggccggca ccgtggctga
     4681 ccgagcccgc cccgaggaac agcaaggcct tgaagaaggc gtgggtgaac agatggaaga
     4741 ccgccgcggt ccaggcgccg acgccgagag ccatgaccat gtatccgagc tggctgatcg
     4801 tcgagtacgc cagcaccttc ttgatgtcgg tctggacgaa ggcgagcacc gcgccgatca
     4861 cgatcgtgac gccgccgacg agggccatga ggttgacgcc gccatgcccg atcgagaacc
     4921 cgttgaagaa caccggatag acgcgagcgc cgaggaagac cccggcgacg accatcgtcg
     4981 cggcatggat gagcgccgaa accggggtcg ggccggccat ggcgtcgggc agccaggtat
     5041 gcagcgggaa ctggccgctc ttgcccatga ttccgatcat caggcagatc gcggcagcca
     5101 ggaggaggtc gtggtgggcg tgagcaccgc tggcgtagag gttcgtcgcg ccgatcccga
     5161 acgacccaac cgtgaagaac aagatgatga tgccgatcat cagcccgatg tcgccgatgc
     5221 gcgtggtgag gaacgccttg atcgcggcgc ggctgttctc gggctcctcc caccagtggc
     5281 cgatgagcat gaacgagcac accccgacga gctcccagcc caccaagagc tggatcaggt
     5341 tctccgccac gaccagcgtc agcatcgacg cagtgaagag cgaaagtgcg
//
LOCUS       DATCCH010000265         2215 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_278013, whole genome shotgun sequence.
ACCESSION   DATCCH010000265 DATCCH010000000
VERSION     DATCCH010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278013"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="VIJ44_05140"
     CDS             complement(<1..661)
                     /locus_tag="VIJ44_05140"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY5165318.1"
                     /translation="MLPPEAAVAVLDRAPDPTPIVASRPAPARVRWSRSWRLFGILLL
                     FTFALRLPAFFVDVFNSDETFLATQAHVIRSGGDLYQEAADRKPPLVPYLYAASFEFF
                     GSTALWTVRLVAMLAIAFTAWLLAVEARRRWGVRASWIAGLLCVFAMVAFAPQDGQAA
                     NFEVFMLPAMTAAVLLARRGRATSSGAAVALATLAKQTGAATLLPVLYLVWKARGKKG
                     LS"
     gene            complement(727..>2215)
                     /locus_tag="VIJ44_05145"
     CDS             complement(727..>2215)
                     /locus_tag="VIJ44_05145"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY5165319.1"
                     /translation="RGVAPGDHVDLLGPTSRLLVTTIQATWLAGATLVCLPLPMRLGS
                     IEEFAAQTRRRIGHAEAKIVVVDPELAPFLVDEPGDPPVVGLDELQGIARDWERPPDD
                     PDALALVQFTSGSTADPKGVMLPHRCIVENIDAIVEGAGLGPDDRGVSWLPLYHDMGL
                     IGLLMTPMLTGFDLVLGAPTDFLAAPADWLRWMSDFRGTVTCAPNFGYALAARAMRRV
                     DNIDLSSWRLGLNGAEPIDPTSVEAFLEAGARHGLDPNTAFCVYGMAEATLAISFPTP
                     GTGMTVDTVDPDVLERFGYAAPVDDGPGRRLARLGRPLRGMEVRVCDPETGQRRGERE
                     VGELELRGASVTPGYFRRDDVTAATFRDGWLRTGDLGYLVDGEVVACGRIKDVIIIGG
                     RNVFPEEIERAVASVDGVRPGNVIAFGTEGRRGKEGIVVVAETKIDDLAAINDQVVDR
                     VCDAVGVPPREVVLVRAGSLPKTSSGKLQRSRCRDQYLDDALAAP"
BASE COUNT          346 a          766 c          820 g          283 t
ORIGIN      
        1 gggagaggcc cttcttgccg cgcgccttcc agacgagata caggaccggc agcagcgtcg
       61 ccgccccggt ctgtttggcg agcgtcgcga gggcgaccgc ggccccagag gacgtggcgc
      121 gcccgcgccg cgctaagagc accgcggcgg tcatcgccgg cagcatgaac acttcgaagt
      181 tcgcggcttg gccgtcttgg ggcgcgaacg ccaccatcgc gaacacgcag agcagcccgg
      241 cgatccagct cgctcgtacg ccccatcgtc ggcgggcttc cacggcgagc agccaggcgg
      301 tgaacgcgat ggcgagcatc gccaccaggc gcaccgtcca cagcgcggtc gacccgaaga
      361 actcgaacga agccgcgtac aggtacggga cgagcggagg cttgcggtcg gcggcttcct
      421 ggtagaggtc gccgccggag cggatcacgt gcgcctgggt ggcgaggaac gtctcgtcgg
      481 agttgaacac gtcgacgaag aaggcgggga gccgcagggc gaaggtgaag aggagcagga
      541 tcccgaagag gcgccaggag cggctccagc ggacccgggc cggcgccggg cgcgacgcga
      601 cgatcggtgt cgggtccggg gcgcggtcga ggacggcgac cgccgcttcg gggggaagca
      661 tccggacatt cttgtcgctc ccgacgaagc gtgccgggcg gggccttccg ggctcggaca
      721 ccccggttac ggggcggcga gggcgtcgtc gaggtattga tctcgacagc gggaccgctg
      781 cagcttcccc gacgatgtct tgggcaggct cccggcccga accaggacca cctctcgggg
      841 tgggacgccg acggcgtcac agacccggtc gacgacctgg tcgttgatcg cggcgaggtc
      901 atcgatcttc gtctccgcga cgacgacgat gccctctttg ccgcggcggc cctcggtccc
      961 gaaggcgatc acgttgccgg gccgcacccc gtcgaccgag gcgaccgccc gctcgatctc
     1021 ctccgggaac acgttacggc ccccgatgat gatcacgtcc ttgatccgcc cgcaggccac
     1081 gacctcacca tcgacgaggt acccgagatc accggtgcgg agccagccgt cacggaaggt
     1141 ggcggccgtg acgtcgtcgc gccggaagta gcccggggtc accgaggccc cccgcagctc
     1201 cagctcgccg acctcgcgct cgccgcggcg ctgaccggtc tcggggtcac agacccggac
     1261 ctccatgccg cgaagggggc ggcccagccg ggcgaggcgt cgtccggggc cgtcgtcgac
     1321 cggggccgcg tacccgaacc gctcgagcac gtcagggtcg acggtgtcca ccgtcatccc
     1381 cgtcccgggc gtcgggaacg agatggcgag ggtcgcctcg gccatgccgt agacgcagaa
     1441 cgccgtgttg gggtcgagcc cgtggcgggc gccagcctcg aggaacgcct cgacggaggt
     1501 ggggtcgatc ggctcggcgc cgttcaggcc gagccgccac gacgagaggt cgatgttgtc
     1561 gactcggcgc atggcccgcg ccgcgagcgc gtacccgaag ttcggcgcgc aggtcaccgt
     1621 cccccgaaag tcggacatcc agcgcagcca gtccgccggt gcggcgagga agtcggtcgg
     1681 cgcgccgagc accagatcga agccggtgag catcggggtc atcagcagcc cgatgagacc
     1741 catgtcgtgg tagagcggca gccacgacac gccgcggtca tcgggtccga gcccggctcc
     1801 ctcgacgatg gcgtcgatgt tctcgacgat gcaccgatgg ggcagcatca cgcccttcgg
     1861 gtcggcggtc gacccgctgg tgaactgcac cagcgcgagc gcgtcggggt cgtcgggggg
     1921 gcgctcccag tctcgggcga tgccctggag ctcgtcgagc ccgacgacgg gcgggtcgcc
     1981 gggctcgtcg acgagaaacg gcgccaactc ggggtcgacg acgacgatct tggcctcggc
     2041 gtgtccgatc cggcggcgag tctgggccgc gaactcctcg atggacccga gccgcatcgg
     2101 caacgggagg cacaccagcg tcgcgccggc cagccacgtc gcctggatgg tcgtcaccag
     2161 cagacgggag gtggggccga gcaggtcgac gtgatcaccg ggggcgaccc cgcgg
//
LOCUS       DATCCH010000266         2466 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_486119, whole genome shotgun sequence.
ACCESSION   DATCCH010000266 DATCCH010000000
VERSION     DATCCH010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486119"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1606)
                     /locus_tag="VIJ44_05150"
     CDS             complement(<1..1606)
                     /locus_tag="VIJ44_05150"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEY5165320.1"
                     /translation="MRRPGPLLSQFLTTIVIGGVAIGACLAALLPGTAVFATSIKYQS
                     DAIAKLRNLAQRSTIYDANLQQIGVLGTQNREDVKLDAVPKILQDAIISVEDKTFWTN
                     DGIDLNGVFRAAVRNVSSGQVVQGGSTITQQLVKERILNSKRDVHRKIQEIILALRLT
                     QKFSKRQILEQYLNTIYFGQGSYGVKAASERLFLVADPASPFGVRGTDLGALSVGQAA
                     LLAGLINNPEGNNPFANPRGAKIRRSVALSRMVTEGYITQADEARGNAEALPTIKPPA
                     DLRPTNAWVAEIQQRLFTDPVYSALGKTVKERKDAVLKGGLKIIATEDTHLQDLAQNA
                     VNNDLSSPAGFTGSLVAIDPKTGEVKAMVGGPGFQNSQFNIATLYPGRQAGSTWKTIT
                     LAAALESGFSPNDIVDGTSPCVFPQWGKTQNAEPGGGAMPLRAATANSVNCAFARTEL
                     AVGFPKLMDTAHKLGITQNTLKPILTLTLGTVESTALEMATVSATIAAEGVHHSPVFV
                     SKILGPDGQVLFDDKNVPGTQVISKDT"
     gene            1714..>2466
                     /locus_tag="VIJ44_05155"
     CDS             1714..>2466
                     /locus_tag="VIJ44_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165321.1"
                     /translation="MSPPEVQRGGLPVPTVAPAGRPARQALWDLIRDVKHDDALGPVT
                     VAVPSPYAGLSLRRDLGRRAGLVNVRFLPLARIAELLGAPRLAEAGRVPLTGARRDEA
                     VHAALRATPGPFAAVATQRSTEERLRATFADLRRAPDSTLEALTRRGSRTAAVAVLYQ
                     HFRALTASSYDEEDLAFAAAEAVRVGAPSLEELGTVILFLPRTLSVGESALAVALAAA
                     GRLLVVESRAATDEDPAPVADRILQAADPDDEA"
BASE COUNT          345 a          863 c          833 g          425 t
ORIGIN      
        1 cggtgtcctt gctgatcact tgggtgccgg ggacgttctt gtcgtcgaag aggacctggc
       61 cgtcggggcc gaggatcttg ctcacgaaca ccggcgagtg gtgaacgcct tccgcggcga
      121 tggtcgcaga cacggtcgcc atctccaacg ccgttgactc cacggtcccc agggtgaggg
      181 tgaggatggg cttcaacgtg ttctgggtga tgcccagctt gtgggcggtg tccatgagct
      241 tcgggaaccc gaccgcgagc tcagtccggg cgaacgcgca gttcacagag ttggctgtgg
      301 cggcgcgcag cggcatggcg cctccgcccg gctcggcgtt ctgggtcttg ccccactgcg
      361 ggaacacgca cggcgatgtc ccgtcgacga tgtcgttcgg cgagaaaccc gattcgaggg
      421 ccgccgcgag cgtgatcgtc ttccacgtcg acccggcttg gcggccgggg tagagggtgg
      481 cgatgttgaa ctggctgttc tggaagccgg gcccgcccac catcgccttg acctcaccgg
      541 tcttcgggtc gatcgcgacc agcgacccgg tgaacccggc cggcgacgac aggtcgttgt
      601 tcacggcgtt ctgcgcgagg tcctggaggt gggtgtcctc ggtggcgatg atcttcaacc
      661 cgcctttgag caccgcgtcc ttgcgttcct tgaccgtctt gccgagcgcc gagtacacgg
      721 ggtcggtgaa cagtcgttgc tggatctctg cgacccatgc gttcgtgggg cgcagatccg
      781 cgggcggctt gatggtcggg agcgcctccg cgttgccccg ggcctcgtcg gcctgggtga
      841 tgtagccctc ggtcaccatg cggctcaacg ccaccgaccg ccggatcttc gcgcccctcg
      901 ggttcgcgaa cgggttgttg ccctcagggt tgttgatcag accggccagg agcgcggcct
      961 gcccaaccga gagcgcgccg aggtcggtgc cacgaacccc gaacggcgac gccgggtcgg
     1021 cgaccaagaa caaccgctcc gaggcggctt tgacgccgta ggagccttgc ccgaagtaga
     1081 tggtgttcag gtactgctcg aggatctgac gcttcgagaa cttctgggtc aggcgcaacg
     1141 ccaggatgat ctcttggatc ttgcggtgga cgtcccgctt cgagttcagg atccgttcct
     1201 tgacgagctg ctgggtgatc gtggagccac cctggacgac ctggcccgac gagacgttgc
     1261 ggaccgcggc gcggaacaca ccgttgaggt cgatgccgtc gttggtccag aacgtcttgt
     1321 cctccaccga gatgatcgcg tcctggagga tcttgggcac cgcgtcgagc ttgacgtcct
     1381 cacggttctg ggttccgaga accccgattt gctggaggtt ggcgtcatag atcgtcgagc
     1441 gctgagcgag gttgcggagc ttggcgatcg cgtcgctctg gtacttgata ctggtcgcga
     1501 agaccgcggt cccgggcagg agggcagcca gacacgcccc gatcgcgacc cctccgatca
     1561 cgatggtcgt caagaactgc gagagcagcg gcccaggcct gcgcatgaag cctccagatt
     1621 ggcacagcga taccccgatc ttgcgttcgc gcccgctgtg cttgacctgt gggcccggcc
     1681 cggggtgtgg caccccctcg gtagcctccg caggtgagcc caccggaggt ccagcgggga
     1741 ggcctcccgg tcccgacggt cgccccagcc ggccgacccg cacgccaggc cctctgggac
     1801 ctgatccggg acgtcaagca cgacgacgcg ctcggccccg tcaccgtggc ggtcccgtcg
     1861 ccgtacgccg gcctgtcgct gcgccgggac ctcgggcgac gagcggggct cgtcaacgtg
     1921 cggttcctcc ccctggcccg gatcgccgag ctgctcggcg cccctcgcct cgccgaggcc
     1981 ggccgggtcc ccctcaccgg ggcacgccgc gacgaggccg tccacgccgc gttacgcgcc
     2041 acgcccgggc cgttcgccgc cgtcgccacc caacgaagca ccgaggagcg cctccgcgcc
     2101 accttcgctg atctgcgccg cgcacccgac tccacgctcg aagcactcac ccggcgcggg
     2161 tcgagaaccg ccgccgtcgc ggtcctctac cagcatttcc gtgcgctgac cgcctcgtcc
     2221 tacgacgagg aagacctcgc cttcgcggcc gccgaagcgg tccgcgtcgg cgcaccctcc
     2281 ctcgaggagc tcggaaccgt catcctcttt ctcccccgca ccttgtcggt gggcgaatcg
     2341 gcgctcgcgg tcgcgctggc cgccgcgggt cgcctcctcg ttgtcgaatc cagggccgcg
     2401 accgacgaag acccggcgcc ggtcgcggat cggatcctcc aggccgccga ccctgacgac
     2461 gaggcc
//
LOCUS       DATCCH010000267         3955 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_221906, whole genome shotgun sequence.
ACCESSION   DATCCH010000267 DATCCH010000000
VERSION     DATCCH010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221906"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..877)
                     /gene="recF"
                     /locus_tag="VIJ44_05160"
     CDS             complement(<1..877)
                     /gene="recF"
                     /locus_tag="VIJ44_05160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HEY5165322.1"
                     /translation="MHLAKLELTDFRCYPAARLEFPPGVILVTGANGQGKTSLLEAVA
                     WSAISRSFRGVPDNALVRAGADNAYLRAAVVDDDATRRVDVEIKRVGRNRILLNGHPT
                     ARTRDLFGTLRVTVFAPDDLQLVKGGPAERRTYLDDLLTASVPRYAGVRADVERVLRH
                     RNALLRGGVRDQEARDTLTVFDDQLVQAGGELIRGRLRLVARLAPAVDDAYRLLATDS
                     PGIHAAYEADWSEGELSAETVEERLRAGLERRRRQETDRGVTLAGPHRDEWRLTIADH
                     DSRTHASQGEQRTLAL"
     gene            complement(936..2039)
                     /gene="dnaN"
                     /locus_tag="VIJ44_05165"
     CDS             complement(936..2039)
                     /gene="dnaN"
                     /locus_tag="VIJ44_05165"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEY5165323.1"
                     /translation="MKFRCERDTLADAVATAYRAVASRTGALPVLSGLLLRAEAGHVE
                     LVGSDLELTIRVQVPAEVDGEGSAVLVARLLQELMSRLAPGVVQVEIGDDQAKIEAGR
                     FRSEIRTLAASDFPRLPEPEGEGVKVAAGPLSQGLRQVVPAASRDDARPILTGVLIAA
                     TADGVRLVATDSYRLAVRDLPGVSLLTEGRSVLVGAKGLGELQRLLPSEGEVEVFLGE
                     RDVTFRLDGTQLTARLVEGEFPNYQQLVPSGYPNKLTVERDALADAVNRVRLVGGQNR
                     DSSPIRLAMNAAGLELSATAQDVGQSYEPVEAKYEGGELTVAFNSQFLLEGLDAAGSP
                     EIAIETVDPLKPAVLRGSESADFLYLLMPVRIA"
     gene            complement(2281..3711)
                     /gene="dnaA"
                     /locus_tag="VIJ44_05170"
     CDS             complement(2281..3711)
                     /gene="dnaA"
                     /locus_tag="VIJ44_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011601270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEY5165324.1"
                     /translation="MDNRERTDPPSDDDLADRIWEQARTRVRAELAEAAWHAWFPGVR
                     PLRFEHDVLVLGVPNTIAAERITSQYLPIVETALLQATGRGLAVELQVDTEARVDEAL
                     ALLASELPKVEPEPSSAPSPQVSDTPWASTTLNPRYTFDQFVIGASNRFAHAAALSVA
                     EAPAQAYNPLFIYGPAGLGKTHLLHAIGHHVRALFPKMRVRYLTTESFMNDFVDAIRD
                     RTRMPVFKRRYRDLEVLLIDDIQFLERTQELQEEFFHTFNQIHNAGGQIIISSDRSPK
                     SIATLEDRLRSRFEWGLITDVQPPEFETRLAILRKKAEAEHLHGIPDEVLAFIASSIH
                     DNIRELEGALIRVAAYSSLNRAPLSEEVARSVLADLLPATKSREITSDLILDETAKQF
                     GWTVEDLCGKSRRRPLVNARQIGMYVMRELTDFSYPRIAQAFGGRDHTTVMYAVDKIR
                     GQMTERHHTFEQVTELIARIRLGSSN"
BASE COUNT          625 a         1225 c         1420 g          685 t
ORIGIN      
        1 ccaacgcaag ggttcgctgc tcgccttggg acgcgtgggt gcggctgtcg tgatcagcga
       61 tggtgagtcg ccactcgtca cggtgcggac cggcgagcgt caccccgcgg tccgtctctt
      121 ggcggcgccg acgttcgagg ccggcgcgca gacgctcctc gacggtctcg gcgctgagtt
      181 cgccttcgct ccagtccgct tcgtaagccg cgtggatacc agggctgtcg gtcgccagga
      241 gccggtacgc gtcgtcgacc gcgggcgcga ggcgcgccac caggcgaaga cggccccgga
      301 tgagttcgcc gccggcctgg accagctgat cgtcgaacac ggtgagggtg tcgcgtgctt
      361 cttggtcccg aacgcccccg cgcaggaggg cgttgcggtg ccgcagcacc cgttccacgt
      421 cggcccgcac gccggcgtac cggggaacag atgcggttag gaggtcgtca agatacgtgc
      481 ggcgttcggc cggtccaccc ttcacgagtt ggaggtcgtc gggtgcgaag accgtgactc
      541 gaagggtgcc gaacaggtcg cgggtacgtg cagtggggtg gccgttcaag aggatccggt
      601 tccggcctac ccgtttgatc tccacgtcga cacgtcgggt ggcgtcgtcg tcgacgacag
      661 cggcgcgcag atacgcgttg tccgcgcccg cgcggaccag cgcgttgtca gggacacctc
      721 ggaacgatcg ggagatcgcc gaccaggcga cagcttccag caggctggtc ttgccttgac
      781 cgttcgcgcc ggtgaccagg atcacaccgg gtggaaactc gagccgagct gcgggatagc
      841 agcggaagtc ggtgagttcg agcttcgcca agtgcactca acgatcctgc cgtgcgggcc
      901 cgggcgctgg cgcgtgtccc cagttggcgc cggcgtcagg caatccgaac cggcatcagg
      961 aggtacaaga agtcggcgct ctcgctgccc cgcagcaccg ctggcttcag tgggtcgacg
     1021 gtctcgatgg cgatctcggg cgagcccgcg gcgtcgaggc cctccagcaa gaactgggag
     1081 ttgaacgcca ccgtgagttc ccctccctcg tatttggctt ctaccggctc gtacgactgg
     1141 ccaacgtctt gggcggtcgc ggacagctcc aggccggcgg cgttcatcgc caaccggatg
     1201 gggctggagt ctcggttctg gccaccgacg aggcggactc gattcaccgc gtcggcaagc
     1261 gcgtcacgct cgacggtcag cttgttcgga tacccgctcg gcaccagctg ctggtagttc
     1321 gggaactctc cttcgacgag acgcgccgtg agctgggtgc catcgaggcg gaacgtcacg
     1381 tcgcgttccc cgaggaacac ctcgacctcg ccttcgctgg ggaggagccg ctgaagctcc
     1441 ccaagcccct tcgcccccac gagcacggat cgtccttcag tcaacaagct cacacccggg
     1501 aggtcacgca ccgcgaggcg atacgagtcg gtggcgacga ggcgaacccc atcggcggtc
     1561 gcggcaatga ggaccccggt gaggatcggg cgcgcgtcgt cgcgtgacgc tgccggcacg
     1621 acctggcgca gcccctgcga gagtggaccg gccgcgacct tgactccttc gccctctggt
     1681 tcgggaaggc gcgggaaatc cgaggcggcc aacgtgcgga tctcggaacg gaaccggccg
     1741 gcttcgatct tggcttggtc gtcgccgatc tcgacttgga ccactccggg ggcgaggcga
     1801 ctcatcagct cctggagcag cctggcaacg agcaccgcgc ttccttcgcc atcgacctcg
     1861 gccggcacct ggacccggat cgtcagttcg aggtcggagc ccaccagctc gacgtgcccg
     1921 gcctcggccc gaagcagcag accggagaga acgggaagcg ccccggtgcg tgacgccact
     1981 gcgcggtacg cggtggctac cgcgtcagca agggtgtcgc gctcgcagcg gaacttcacg
     2041 gctgctcctt ccgaggtgga gttactactt ctatatgtac caaggttcgt cgtcgtagta
     2101 ggggctgtgg attgtggatg atgtgacgcg gtcccaggtc agtggctgaa tcgtcgtccc
     2161 cagggagaac ggcgaattgt ggggaacgcc tggttctcag gggatctggg tggagcaccg
     2221 cacggtatcc aaggttgggg gcaggctttc cccagctctc cacagcgttg tgcacacgtc
     2281 tcagttgctg ctgcccaatc ggatgcgggc aatcagttcg gtcacctgct cgaaggtgtg
     2341 gtgccgttcg gtcatctgac cgcggatctt gtcgacggca tacatgacgg tggtgtgatc
     2401 tcgcccgccg aaggcctggg cgatgcgggg gtagctgaag tcggtgagct cccgcatcac
     2461 gtacatgccg atctggcggg cgttcaccag tggccggcgg cgactcttgc cacagaggtc
     2521 ctcgacggtc catccgaact gcttggcggt ctcgtccagg atgaggtccg acgtgatctc
     2581 gcgggatttg gtcgccggga ggaggtcggc cagcacggag cgggccactt cctcggagag
     2641 gggcgcccgg ttaaggcttg agtacgcggc cacccggatc agggcaccct cgagctcgcg
     2701 gatgttgtcg tggatgctgc tggcgatgaa ggccaggacc tcgtcgggga ttccgtggag
     2761 atgctcggct tcggccttct tgcggaggat ggccaggcgg gtctcgaact cgggcggttg
     2821 cacgtcggtg attagccccc actcgaaccg acttcgcagc cggtcctcga gggtggcgat
     2881 ggacttcggt gaacggtcgg aggaaatgat gatctgtccg cccgcgttgt ggatctgatt
     2941 gaacgtgtgg aagaactcct cctggagttc ttgggttcgc tccagaaact ggatgtcgtc
     3001 gatgaggagg acttcaaggt cgcggtagcg gcgcttgaag accggcatcc gggttcggtc
     3061 ccggatggcg tcgacgaagt cgttcatgaa cgactcggtg gtgaggtatc ggacccgcat
     3121 cttggggaac agggcccgga cgtggtgtcc gatggcgtgc agcagatggg tcttgcccag
     3181 gccggccggc ccgtagataa acaacgggtt gtaagcctgg gcgggcgctt cggcgaccga
     3241 gagcgccgcg gcgtgggcaa accggttgga ggcgccgatc acgaactggt cgaaggtgta
     3301 acggggattg agggtcgtcg aggcccacgg ggtgtccgac acctgggggc tgggggccga
     3361 gctcggctcg ggttcgactt tggggagctc cgaggccagg agggcaaggg cctcatcgac
     3421 gcgggcttcg gtgtccacct ggagctccac cgcgaggcct cgaccggtcg cttggaggag
     3481 ggcagtctcg acgatcggga ggtactggga tgtgattcgc tccgcggcga tggtgttcgg
     3541 gactccgagc accaacacgt cgtgttcgaa ccgcaacggc cggaccccag ggaaccaggc
     3601 gtgccaggcc gcttcagcca actcggcgcg gacccgtgtc cgggcttgtt cccagatccg
     3661 atcggccaga tcgtcgtcgg acggcgggtc cgtccgctct cggttgtcca caggcgcttc
     3721 cacaggtgtg gagaacggga gtggcgccgg ggcgccttcc ctctcagggt ggtcggccgg
     3781 ttgaggcggt cgcccctggg gaagtggtgt cgtcaacagg ccgggttgac ggcgaacgcg
     3841 gaacgtagca ccgggcgcgc gccccgacaa gaggtgaaca ggagccgacc aggcagcgcg
     3901 gcgcgcgggc gcgaagccac gcgttgcggg taccgcaacc cgcccgtaga ctcag
//
LOCUS       DATCCH010000268         2497 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_138879, whole genome shotgun sequence.
ACCESSION   DATCCH010000268 DATCCH010000000
VERSION     DATCCH010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138879"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..87)
                     /locus_tag="VIJ44_05175"
     CDS             complement(<1..87)
                     /locus_tag="VIJ44_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005264599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HEY5165325.1"
                     /translation="MVRLVYSAIMSLDGYVADAGGGFDWAAPD"
     gene            complement(98..862)
                     /locus_tag="VIJ44_05180"
     CDS             complement(98..862)
                     /locus_tag="VIJ44_05180"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012888494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEY5165326.1"
                     /translation="MKRLEGRVALITGGASGIGLASAQRLASEGATIVVVDMNAHAGT
                     SIAAELGGLFVSADVTSPEDNERMYATAFDTYGRIDIAFHNAGISPPDDDSILVTGLD
                     AWRRVQEVNLTSVFLGCKAVIPYMQRAGKGSIINTASFVATMAAATSQISYTASKGGV
                     LAMSRELGVQFAREGIRVNALSPGPVNTPLLQELFAKDPERAARRLVHIPFGRFAEPS
                     EIAAAVAFLASDDSSFITAANFLVDGGISGAYVTPL"
     gene            complement(859..2238)
                     /locus_tag="VIJ44_05185"
     CDS             complement(859..2238)
                     /locus_tag="VIJ44_05185"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013223703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY5165327.1"
                     /translation="MTVMHEVVNPATEEIVTTVAHADAAGADEAIAVAQQAFPAWREV
                     APADRGRLLRRFSEQVDAHLEELAQLEVANSGHTIGNARWEAGNVRDVLAYYSAAPER
                     MFGRQIPVAGGVDITFKEPLGVIGVIVPWNFPMPIAGWGFAPALAAGNTVVLKPAELT
                     PLTAIRLGELAVEAGIPEGVFTVIPGKGSIVGQRFVDHPAVRKVVFTGSTTVGKQVMR
                     GCADQVKRVTLELGGKSANIVFADADLEQAAATAPYGVFDNAGQDCCARSRILVQRSA
                     YERFMELLEPAVKGVRVEDPTLDTSEMGPLVSRGQWETVASYVPEGSPVAFTGTAPEG
                     PGFWYPPTVLAPVSEHDRTVREEIFGPVVVVLPFDDEADAIRIANDSEYGLSGSIWTQ
                     NLGRAMRVSRAVEAGNLSVNSHSSVRYSTPFGGYKQSGLGRELGPDAVDAFTETKNVF
                     FSQAGAGSS"
     gene            complement(2244..>2497)
                     /locus_tag="VIJ44_05190"
     CDS             complement(2244..>2497)
                     /locus_tag="VIJ44_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016827016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEY5165328.1"
                     /translation="GLDGIERGLELEPAFGGNAYDSGAERVPDSLGKALELWESSEWV
                     RETFGDDVVAHYANMARVELDAFGRSITDWERFRSFERM"
BASE COUNT          395 a          902 c          830 g          370 t
ORIGIN      
        1 gtccggcgcc gcccagtcaa acccgccgcc agcgtccgcc acgtagccgt cgagcgacat
       61 gatggccgag tagaccagcc tgaccatccc gaccccctta cagcggcgtg acgtacgccc
      121 cggagatgcc gccgtccacc aggaagttgg ccgccgtgat gaacgacgag tcgtcgctcg
      181 cgaggaaggc gaccgccgcc gcgatctccg acggttccgc gaagcggccg aacgggatgt
      241 gcaccagccg ccgcgccgca cgctccgggt ccttggcgaa cagctcctgc agcagggggg
      301 tgttgaccgg gccagggctc agcgcgttga cccggatgcc ctcgcgggcg aactgcacgc
      361 cgagctcgcg ggacatcgcg agcaccccgc ccttggatgc ggtgtacgag atctgcgacg
      421 tcgccgccgc catcgtcgcg acgaacgacg ccgtgttgat gatcgagccc ttcccggccc
      481 gctgcatgta cgggatgacc gccttgcagc cgaggaagac cgacgtcagg ttgacctcct
      541 gcacccggcg ccaggcgtcg agcccggtga ccaggatgga gtcgtcgtcg gggggcgaga
      601 tgcccgcgtt gtggaaggcg atgtcgatgc gtccgtaggt gtcgaacgca gtcgcgtaca
      661 tccgttcgtt gtcctcggga ctcgtcacgt cggccgagac gaacaggcca ccgagctcgg
      721 cggcgatcga cgtccccgcg tgggcgttca tgtcgacgac gacgatggtc gcgccctcgg
      781 acgcgagccg ctgcgcggac gccagaccga tcccgctggc accaccggtg atgagggcga
      841 cgcggccttc cagacgcttc atgagctccc agctcctgct tgtgagaaga agacgttctt
      901 ggtctcggtg aaggcatcca cggcgtcggg cccgagctcg cgcccgaggc ccgactgctt
      961 gtacccgccg aacggcgtcg agtagcgcac cgacgagtgg ctgttgaccg acaggttgcc
     1021 ggcctccaca gcacgggaca cacgcatcgc ccggccgagg ttctgggtcc agatggaccc
     1081 ggacaggccg tactccgagt cgttggcgat ccggatcgca tcggcctcgt cgtcgaacgg
     1141 cagcacgacg acgaccgggc cgaagatctc ctcgcgcacg gtccggtcgt gctccgacac
     1201 cggcgcgaga accgttggcg ggtaccagaa cccgggcccc tcgggtgctg tacccgtgaa
     1261 cgcgaccggt gacccctctg gcacgtacga cgcaaccgtc tcccactggc cgcgcgacac
     1321 gagcggcccc atctcggacg tgtcgagggt cgggtcctcg acgcgcacac ccttgaccgc
     1381 aggttcgagc agctccatga accgctcgta cgcggaccgt tgcacgagga tccgcgagcg
     1441 ggcgcagcag tcctggcccg cgttgtcgaa cacgccgtag ggcgccgtcg ccgccgcctg
     1501 ctccaggtcg gcgtcggcga agacgatgtt cgcgctcttg ccgcccagct cgagcgtcac
     1561 ccgcttgacc tggtcggcac agccccgcat gacctgcttg ccgacggtcg tcgaaccggt
     1621 gaagaccacc ttgcgcacgg ccggatggtc gacgaaccgc tgcccgacga tcgacccctt
     1681 cccggggatc acggtgaaca cgccctcggg gatgccggcc tcgacggcca gctcaccgag
     1741 ccggatcgcg gtgagagggg tcagctcggc cggcttcagg acgacggtgt tgcccgctgc
     1801 gagcgccggc gcgaagcccc agcccgcgat cggcatcggg aagttccacg gcacgatgac
     1861 gccgatgaca ccgagcggct ccttgaacgt gatgtctacg ccgccagcca ccgggatctg
     1921 ccgcccgaac atccgctcgg gcgcggcgct gtagtaagcc aggacgtcgc gtacgttgcc
     1981 ggcctcccag cgggcgttgc cgatggtgtg gccggagttg gcgacctcca gctgggccag
     2041 ctcctcgagg tgcgcatcga cctgctcgga gaaccgccgc agcagccggc cgcggtcagc
     2101 aggggccacc tcacgccatg cgggaaaggc ttgctgcgca acagctatcg cctcgtcggc
     2161 tccggcagca tcggcgtgcg ccaccgtggt gacgatctcc tcggtcgccg ggttcacgac
     2221 ctcgtgcatg acagtcacgc cactcacatc cgctcgaagg accggaatcg ttcccagtcg
     2281 gtgatggacc gaccgaaggc gtcgagctcg accctcgcca tgttggcgta gtgggccacc
     2341 acgtcgtcgc cgaacgtctc gcgcacccac tcgctcgact cccacagctc gagcgccttg
     2401 ccgagggagt ccggcacccg ctcggctcct gagtcgtagg cgttgccgcc gaaggccggc
     2461 tcgagctcca gccctcgctc gatcccgtcg agccccg
//
LOCUS       DATCCH010000269         2084 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_346881, whole genome shotgun sequence.
ACCESSION   DATCCH010000269 DATCCH010000000
VERSION     DATCCH010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346881"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VIJ44_05195"
     CDS             complement(<1..260)
                     /locus_tag="VIJ44_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010367705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HEY5165329.1"
                     /translation="MDRAITIAERVRRRTPPNPWVGCVLVRDGQVVGEGATFPPGGAH
                     AEAAALTAAGEQARGATAYVTLEPCAHQGRTEPCTTALIDAGT"
     gene            complement(560..1042)
                     /locus_tag="VIJ44_05200"
     CDS             complement(560..1042)
                     /locus_tag="VIJ44_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HEY5165330.1"
                     /translation="MTLAAKVLTVSDGVAEGTRDDRSGRALTERLTTAGFDVVEHRVT
                     PDGVDAVAAALRDLTQDFTGLVVTTGGTGFGPRDLTPEGTRAVLTREAPGLGEAMRRA
                     SDADGRRFGMLSRGVCGTTGTALVCNLPGSSSGALECLDAVLPVLPHALDLLGGGHPH
                     "
     gene            complement(1039..1704)
                     /gene="rpe"
                     /locus_tag="VIJ44_05205"
     CDS             complement(1039..1704)
                     /gene="rpe"
                     /locus_tag="VIJ44_05205"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018333644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEY5165331.1"
                     /translation="MPGQKLAPSILSADFARLGDEVTAVTPEADLLHVDVMDGHFVPN
                     LTIGPPVVASLRRHTRLFLDCHLMIDNPGELLADFADAGADRCIVHVELGDPRPLFEE
                     LRTRKVQVGLALSPETPFAAAEPYLTDIDLLLVMSVHPGFGGQQFLTEVLDKVRAARQ
                     IIDRDQLPVEIEIDGGVHTATAPLAASAGVDILVAGSAIFRAPDPVAAARALREAAWP
                     TAA"
     gene            complement(1715..>2084)
                     /locus_tag="VIJ44_05210"
     CDS             complement(1715..>2084)
                     /locus_tag="VIJ44_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005202704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165332.1"
                     /translation="LVDAPCSGLGVLRRRPEARWRVGEPSTELVDLQVALVLAAAPTV
                     RPGGRLVYSVCTLTAAETTGVAARVLDTLGDDFAVLDPPPAPWRPWGPGALLLPQDAG
                     TDGMFVLVLRRVPGTIGSGR"
BASE COUNT          269 a          679 c          861 g          275 t
ORIGIN      
        1 gttccggcgt cgatcagggc ggtggtgcac ggctcggtgc ggccctggtg ggcgcacggc
       61 tcaagggtga cgtacgcggt ggcgcctcgg gcctgctcgc cggcggcggt gagggccgcg
      121 gcttcggcgt gggctccgcc gggtgggaag gtggcgccct cccccacgac ctggccgtcg
      181 cgcaccagga cgcagccgac ccaggggttc gggggcgtgc ggcgccggac gcgctcggcg
      241 atggtgatcg cccgatccat cgcctccgtg tcgtccacct tggtcccttc cgcctcgggg
      301 agcgggcagg acggggcgcg cgacaccggc gcaccctctc ccatccggac tgtgaccgtc
      361 ggctccggcg ttggaccgga tcggcgccac gacaggtggc cgtgggctcg cgggctcccg
      421 gcgagcggac cggagtctgc tcgccggcta ccgccggtcg ggacttgcac ccaaaccccg
      481 agggctgttc ttcggggcca ttctagccct cccggccaga aatgaaacgt gttctagttc
      541 ggtgccaggg tgggggcggt caatgggggt ggcctccgcc gaggaggtcg agggcgtggg
      601 gcaggacggg gaggaccgcg tcgaggcatt cgagcgcccc cgatgaggac ccgggcaggt
      661 tgcagaccag tgcggtgccg gtcgtgccgc acacgccgcg cgagagcatg ccgaacctgc
      721 gtccgtcggc gtcgctggcg cggcgcatgg cctcgccgag gccgggtgct tcccgggtca
      781 gtacggcgcg ggtgccctcg ggagtgaggt cccggggccc gaagccggtc ccgccggtgg
      841 tgacgacgag gccggtgaag tcctgggtca ggtcgcggag cgcggcggcg acggcgtcga
      901 caccatcggg ggtgacgcgg tgctcgacga cgtcgaaccc ggcggtggtc agccgttcgg
      961 tgagggcgcg tccggagcgg tcgtcgcggg tgccctcggc gacgccgtcc gagacggtga
     1021 ggaccttggc tgccagcgtc acgccgcggt cggccacgcg gcctcgcgca gggcgcgggc
     1081 cgcggcgacc gggtcggggg cgcggaagat ggcgctcccg gcgacgagga tgtcgacccc
     1141 ggccgacgcc gcgagcggcg cggtggcggt gtggacaccg ccgtcgatct cgatctcgac
     1201 cggcagctgg tcccggtcga tgatctgacg ggcggcgcgc accttgtcga ggacttcggt
     1261 gaggaactgc tggccgccga acccggggtg cacgctcatg accagcagca ggtcgatgtc
     1321 ggtgaggtag ggctcggcgg cggcgaaggg tgtctcgggg ctgagggcca ggccgacctg
     1381 gaccttgcgg gtgcggagct cttcgaagag cggtcggggg tcgccgagct cgacgtggac
     1441 gatgcagcgg tccgcgccgg cgtcggcgaa gtcggcgagg agctcgccgg ggttgtcgat
     1501 catgagatga cagtcgagga acaggcgggt gtggcgccgt agcgacgcga cgaccggtgg
     1561 cccgatggtc aggttcggga cgaagtggcc gtccatcacg tcgacgtgga gaagatcggc
     1621 ctcgggggtc acggccgtga cctcgtcgcc gagccgggcg aagtcggcgg acaggatcga
     1681 cggcgcgagc ttctgtccgg gcacttcgac gacgctaccg cccggatccg atcgttccgg
     1741 gcacccggcg aagcacgagg acgaacatcc cgtcggtgcc ggcgtcctgt ggtaacagca
     1801 gcgcgcccgg tccccagggt cgccacggcg cggggggcgg gtcgaggact gcgaagtcgt
     1861 cgccgagggt gtcgaggacc cgggccgcga cgccggtcgt ctcggccgcg gtgagggtgc
     1921 agaccgagta gacgaggcgc ccgccgggcc gcaccgtcgg ggccgcggcg aggacgagcg
     1981 cgacctgcag gtcgacgagc tcggtggacg gctcccccac tcgccagcgg gcctcggggc
     2041 ggcggcgaag cacaccgagc ccggagcagg gggcgtcgac gagg
//
LOCUS       DATCCH010000270         5105 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_486691, whole genome shotgun sequence.
ACCESSION   DATCCH010000270 DATCCH010000000
VERSION     DATCCH010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5105
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486691"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="VIJ44_05215"
     CDS             complement(<1..410)
                     /locus_tag="VIJ44_05215"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY5165333.1"
                     /translation="MTAPAPTGFDQALRVVADVRGWMTDDQARRLWDRAAVVAPGGQI
                     VEIGSYQGRSAIVLARAAAPEVEVVAIDPHAGNDRGPQEIHGSLTEGERDHQAFLANL
                     DQAGVRDRVRHVRAPSQKATEDVNGPIDLLYIDGA"
     gene            588..1769
                     /locus_tag="VIJ44_05220"
     CDS             588..1769
                     /locus_tag="VIJ44_05220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006542609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY5165334.1"
                     /translation="MTDHDATIAKMGDLAAEAGLRRVSILAWRDLDDPEAGGSEISAA
                     MVCRYWAEAGLEVTMRTSFAAGRPEVHWREGYRVIRKAGRYLVFPRAAISEMLGWHGG
                     RDGLMEVWNGMPFFSPLWARTPHITFIHHLHAEMWQMTLPSRLAAVGNFVESRLAPPF
                     YRHTPIVTPSESSKGELVAAMGFQPRRVTVVHPGVDPRFRPGGERSPHPLVLAVGRLV
                     PVKRHDLLIEALIELKRRHPTLEAVIAGDGYKRDELEAQLHAARAEDWIHLPGRISDE
                     DVVDLYRRAWVLASSSAREGWGMTITEAAAAGTPAVVTRIAGHLDAVADGRSGLLVDG
                     RDGLVDGLDRVLRDPDLRDRLSAGALQHAASMTWEASAFGVLEVLAAEAMRVRARSSP
                     R"
     gene            1766..>5105
                     /locus_tag="VIJ44_05225"
     CDS             1766..>5105
                     /locus_tag="VIJ44_05225"
                     /inference="COORDINATES: protein motif:HMM:NF023275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-(1->3)-arabinofuranosyltransferase family
                     protein"
                     /protein_id="HEY5165335.1"
                     /translation="MSALTADTPTAEPGASPSTRAQRVLGYWLLAGLAYIPVILTAPG
                     RVVVDTKQYLYLDPGRLLARAPFLWQPDVHLGTVTHENIGYLFPAGPFYWVFANLGVP
                     AWVAQRLWFGSILFGAGLGVLYLLRTLHLRGPGVIVAALVYMLSPYTLQYASRLSILL
                     IAWSAMPWMIALAARSLRSDGWRYPAAFAIVVQLAGSVNATTLLYAGLAPVLWIIHSV
                     WISREVSLARALRTTGRIALLTILTSLWWIAGLAVQAGYGLDVLKYTETIKAVSTAGQ
                     AGEVQRGLGYWFFYGGDKLGPWIEAAIDYTSRRWLIFVGYTIPILAMVSAAFIRWRHR
                     VYFVALIAVGVLIAVGAHPYNDPSPFGSIVKSFGEGSTVGLALRSVGRAIPLVVLGFG
                     VLLGVGANLLVRWLARRGQLRIGLAACVGLGILAIVNLPALWNGTFYGNNLQRPETLP
                     KYWTDAIAALDKGSHQTRILELPGADFSAYRWGNTVEPITPGLTDRPYVARELVPYGS
                     AASANLLDALDRRLQLGLLQPSSVAPVARLMSVGDVVLRNDLQVDRYDLARPKNTWMV
                     FNPTPTGLGTPTSYGHGLGPPLALPLLDAKALALPAGTSDPAPVVAFPVHNPVPIVRA
                     DATNPATIVSGDGEGLVDLSQSGLLNGQELLQYSASFAGDPQGLAHAVGTDSVLVVTD
                     SNRKRAERWDNVNYNVGYTQRPGEQPLVKDTNDNQLVVFPGAGENASTVMEQRGVQVS
                     ATGYGALSQYLPASRPARAFDGDVNTSWTVGAFEAVDGARIRADIAHSITTGTVNLVQ
                     PLNGQRDRYITRVNLHFTDAHGQPVGGDVTADLGPASRTATGHTLHYPTRTFSRLEIT
                     VAADNVGPQPNYPHSSGVGFAEIRLHDDHTPSQNLRVDEVVRMPTDLVSAPAARSVTH
                     PLVYEMTRLRTVLAPPNTAEEEAALVRSFTVPVGRDFGLVGTARVSAGAPDQNIDQAL
                     GIPDANAGGITATASEHLTTTAQSRASAAIDGDPTTAWSTQVGDPTGQWLDLKLSQPV
                     TFNHLNLSLVSDGRHSVPARLTIDAGGGQTRVVDVPPVGTTSVENGTVATPVTFPAVT
                     TNHVRVTIDAVRPVNTIEYYSNLPT"
BASE COUNT          768 a         1942 c         1582 g          813 t
ORIGIN      
        1 gcgccgtcga tatagaggag gtcgatcgga ccgttgacgt cctcggtggc tttctgggac
       61 ggcgcacgga cgtgacggac ccggtcacgg acgccggcct ggtccaagtt ggcgaggaac
      121 gcctggtggt cccgctcacc ctcggtgagg ctcccgtgaa tctcttgtgg accccggtcg
      181 ttgccggcgt gcgggtcgat cgcgacgacc tcgacctcgg gagccgcggc acgagcgagc
      241 acgatcgccg agcggccctg gtagctgccg atctcgacga tctgtcctcc gggcgcgacg
      301 acggcggccc ggtcccagag gcggcgggcc tgatcgtcgg tcatccagcc ccgcacgtcg
      361 gcgacgacac gcagcgcctg gtcgaagccg gtcggggccg gtgccgtcac gggatctcct
      421 cggtcatcgg accggtcatt gtggagcctc gggccgggtc cgaccaacac gacccgccac
      481 gctcgacccg cgcctgcggg tcgagctggg cattcgcgta ggctgcctca gccgccctgg
      541 ggctcggctc gcgaggctga gcggggccgg cgccgggcga tcccacgatg accgaccacg
      601 acgccaccat cgccaaaatg ggcgacctcg ccgccgaggc aggactccgc cgggtgagca
      661 tcctcgcctg gcgcgacctc gacgaccccg aagctggcgg ctcggagatc tcggccgcga
      721 tggtctgtcg gtactgggcc gaagccgggc tcgaggtcac catgcggacc tccttcgccg
      781 ccggtcgacc cgaagtccac tggcgtgagg ggtatcgggt catccgaaag gcgggccgct
      841 acctggtgtt cccccgagct gcgatcagcg agatgctcgg atggcacggc ggccgcgacg
      901 gccttatgga ggtctggaac ggcatgccgt tcttctcgcc gctgtgggcc cgcacccccc
      961 acatcacctt cattcaccac ctccacgccg agatgtggca gatgacgttg ccgtcccgcc
     1021 ttgccgcggt gggcaacttc gtcgaatcgc ggctggcgcc accgttctat cggcatacgc
     1081 cgatcgtcac cccctccgag tccagcaaag gcgaactcgt cgccgccatg ggtttccagc
     1141 cccggcgggt gaccgtcgtg caccccggag ttgacccccg cttccgtccc ggcggtgaac
     1201 ggtcaccgca ccctttggtg ctggctgtcg gacggctggt gcccgtgaaa cgccacgacc
     1261 tgctcatcga ggccctcatc gaattgaagc gccgccatcc cacgctcgaa gctgtgatcg
     1321 cgggcgacgg ctacaagcgc gacgagctcg aagcccagct gcacgcggcg cgggccgagg
     1381 actggattca tctcccgggg cgaatctccg acgaggacgt cgtcgatctc taccggcggg
     1441 cctgggtcct ggcgagctct tcggcgcgtg aaggctgggg gatgacgatc actgaggcgg
     1501 cggcggcggg gacccccgcg gtcgtcaccc gcatcgccgg acacctcgac gcggtcgctg
     1561 acggacgaag cgggctgttg gtcgacggcc gggacggcct cgtggacggt ctcgatcgcg
     1621 tcctccgcga ccccgacctc cgcgaccgtc tctcggccgg cgcgctgcag cacgccgcga
     1681 gcatgacttg ggaagcatcc gcgttcggcg ttctggaggt cctggcggcc gaggcgatgc
     1741 gcgtccgcgc tcggtcgtcg ccccgttgag cgcgctgacc gccgacaccc ctaccgctga
     1801 acccggggct tccccgagca cacgcgccca acgggtactc ggctactggc tgctcgccgg
     1861 cctggcgtac atcccggtga tcctcaccgc gccgggacgg gtcgtcgtcg acaccaagca
     1921 gtatctctac ctcgaccccg gccggctgct cgcgcgcgct ccgttcctat ggcaaccaga
     1981 cgtgcacctc ggcaccgtca cccacgagaa cattgggtac ctcttcccgg ccggaccgtt
     2041 ctattgggtc ttcgccaacc tcggggtgcc cgcctgggtt gcccaacggc tctggttcgg
     2101 ctcgatcctg ttcggcgccg ggctcggcgt cctctatctc ctccgcaccc tccacctgcg
     2161 tggacccggc gtgatcgtcg ccgcgctcgt ctacatgctg agcccgtaca cgctgcagta
     2221 cgcgtcgcgg ctctcgatcc tgctcatcgc ctggagcgca atgccgtgga tgatcgcgct
     2281 cgcggcgcgg tcactccgaa gcgacggttg gcgctacccc gcggcgttcg cgatcgtcgt
     2341 ccagctcgcc gggagcgtca acgccaccac cttgctgtac gccgggctcg ccccggtgct
     2401 gtggatcatc cactcggtct ggatctcgcg ggaggtctcg ctggcgcggg ccttgcgcac
     2461 gaccggacgc atcgcgctcc tcaccatcct cacgtcgctg tggtggatcg ccgggctcgc
     2521 cgtccaggcc ggctacgggc tcgacgtcct caagtacacc gagacgatca aagcggtctc
     2581 caccgccgga caggccggcg aagtgcaacg gggcctcggc tactggttct tctacggcgg
     2641 agacaagctc ggcccgtgga tcgaagcggc gatcgactac acgtcgcgac gatggctgat
     2701 cttcgtgggc tacacgatcc cgatcctcgc catggtgtcc gccgcgttca tccggtggcg
     2761 tcaccgggtc tatttcgttg ccctcatcgc cgtcggtgtg ctgatcgcgg tcggggcgca
     2821 cccctacaac gacccctcac cctttgggtc catcgtcaag tccttcggtg agggatcgac
     2881 cgtcgggttg gcgctgcgca gcgtggggcg ggccatcccc ctcgtcgtgc tcgggttcgg
     2941 tgtcctcctc ggagttggcg ccaacctcct cgtccgctgg ctcgcccgac ggggccaact
     3001 gcgcatcggg ctcgcggcct gcgtgggcct tgggatcctc gcgatcgtca atcttcccgc
     3061 gctctggaac ggaacgttct acggcaacaa cctgcagcgg cccgagaccc tcccgaagta
     3121 ctggaccgac gcgatcgccg cgctcgacaa gggatcgcat cagacccgga tcctcgagct
     3181 gcccggcgcc gacttctccg cctaccgctg gggcaacacc gtcgagccga tcacccccgg
     3241 actgaccgac cgcccctacg tggcgcgaga gctcgtcccg tatgggtccg cggcgtcagc
     3301 gaacctcctc gacgcgctcg accgtcgact ccagctgggc ttgctccaac cttcctcggt
     3361 ggcgccggtg gcgcggctca tgagcgtcgg cgacgtcgtc ttgcgcaacg accttcaagt
     3421 tgatcgctac gacctggccc gaccgaagaa cacctggatg gtgttcaacc cgacaccgac
     3481 cggcctgggg acgcccacgt cgtacgggca tggtctcggc ccgccgctgg ctctgcccct
     3541 tctcgacgcg aaggcgttgg ctctcccggc cggcacgtcc gatccggcgc cggtcgtcgc
     3601 gttcccggtc cacaacccgg tacccatcgt gcgagccgac gcgacgaatc ccgcgacgat
     3661 cgtctcgggt gacggcgaag gcctcgtcga cctgtcccag tcgggactgc tcaacggcca
     3721 ggagctgctg caatattcgg cgtcgttcgc cggtgacccc cagggactcg cacacgcggt
     3781 cggaaccgac agcgtcctgg tcgtcaccga cagcaaccgc aaacgcgcgg agcgttggga
     3841 caacgtcaac tacaacgtcg gctacaccca gcgtcccggt gaacagccct tggtgaagga
     3901 cacgaacgac aatcagctcg tcgtcttccc cggcgcaggc gaaaacgcct ccaccgtcat
     3961 ggagcagcga ggcgtccagg tgtccgccac cggttacggc gccctctccc aatacctccc
     4021 ggcgagccgt cccgcacgcg cctttgacgg cgacgtcaac acctcctgga cggttggcgc
     4081 gttcgaggcc gtcgacggcg cccggatccg cgccgacatc gcccactcca tcacgaccgg
     4141 caccgtcaac ctcgtgcaac cgctgaacgg ccaacgagac cggtacatca cccgggtgaa
     4201 cctccacttc accgacgctc acggacaacc cgtcgggggc gacgtcaccg ccgatctcgg
     4261 accggcgtcg cgaaccgcca ccggccatac cctccactac ccgactcgaa cgttctcgcg
     4321 cctcgagatc accgtcgccg ccgacaacgt cggacctcag ccgaactacc cccattcgag
     4381 tggcgtcggc ttcgccgaga tccgcctgca cgacgaccac accccatccc agaacctccg
     4441 ggtcgacgag gtggtgcgca tgcccacgga cctggtgagc gcacccgcgg cccgttccgt
     4501 cacccatccc ctggtctatg aaatgacccg gctccgcacc gtcctcgcgc cgcccaacac
     4561 cgccgaagaa gaagcagcgc tggtccgctc gttcaccgtg cccgtcggac gcgacttcgg
     4621 gctcgtcggc actgcccggg tctcggcggg agcccccgac cagaacatcg accaggcact
     4681 cggcatcccc gacgccaacg ccggtggcat caccgcgacc gcatccgagc acctgaccac
     4741 gaccgcacag tcccgcgcct ccgcggcgat cgacggcgat ccgaccaccg cctggagcac
     4801 ccaagtcggt gaccccaccg ggcaatggct cgacctcaag ctgtcccagc ccgtgacctt
     4861 caaccacctc aacctgagcc tcgtctccga cggccgccac tccgtcccgg cccgactcac
     4921 tatcgacgcc ggcggcggtc agacccgcgt cgtcgacgtc ccaccggtcg gcaccacctc
     4981 ggtcgagaac ggcaccgtcg ccacgcccgt caccttcccg gccgtcacca ccaaccacgt
     5041 gcgcgtcacc atcgacgccg tccgcccggt gaacaccatc gagtactact ccaacctccc
     5101 cacca
//
LOCUS       DATCCH010000271         3214 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_113095, whole genome shotgun sequence.
ACCESSION   DATCCH010000271 DATCCH010000000
VERSION     DATCCH010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113095"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="VIJ44_05230"
     CDS             <1..304
                     /locus_tag="VIJ44_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165336.1"
                     /translation="RRAGGLGHDGVFKLRQALIAVTANKIYGFNSKPKGRKGWQIVDQ
                     VAEWDRRDVRVSFDDQRITRTVTLEIISTGVLHELEMVQLAGALNDPLFAALAVTP"
     gene            complement(416..844)
                     /locus_tag="VIJ44_05235"
     CDS             complement(416..844)
                     /locus_tag="VIJ44_05235"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY5165337.1"
                     /translation="MIAQLPAGFASAIDGVRHSELQRRPVATMWSIAEYLDHVRETTF
                     GMRFILATALEASGTDVGEPPASRFDPEPRLVDVRAALKGLREEAQQLHDQLRQLGEP
                     SWESTVVVGGEVVDVHWIARHAVHDATHHLSDIERLRERL"
     gene            complement(990..1679)
                     /locus_tag="VIJ44_05240"
     CDS             complement(990..1679)
                     /locus_tag="VIJ44_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006604084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spheroidene monooxygenase"
                     /protein_id="HEY5165338.1"
                     /translation="MIASVHLADVGARSALSILRKPPDPGAITGLRHANAALAAPLRG
                     SFRPAPDPGRIGLVAFWDSDDALDEFLADDPLAAKLARGWHVRLEPLRAFGSWPGLPD
                     NVPTARATDYEGPAAVLTLGRLRLSQAIRFLRVSAKAEGAVVEAPGLTWATGLGRPPF
                     VGTCSLWQTTQALSTYAYGHPKPAHPDAITADRAKPFHHQSAFIRFRPYGSRGQLDGR
                     NPLVESWMSTT"
     gene            complement(1917..2708)
                     /locus_tag="VIJ44_05245"
     CDS             complement(1917..2708)
                     /locus_tag="VIJ44_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEY5165339.1"
                     /translation="MTGWTSALGFEPHTNEQFDDPRYRWRRRFSELFGTFLLVLAAAG
                     GPVVNAVSHGRVSLSAQVVAPGLMVMAVIYFMGAVGGAHLNPAVTLSFALRGNFPWQR
                     VPGYVGMQLVGAVVAAAFLRAMFGNVGHLGATLPMHGISDVKVFLLEAVLTLGLVSVI
                     LGTASGGRNIGPNAALAVGAYIALAGLWAAPISGASMNPARSFGPAVLGGHWTAFWAY
                     VIGPIVGGLVAVGFAWILRGPPTKAGTKAAQGSTGTRRSKKHSRS"
     gene            2844..>3214
                     /locus_tag="VIJ44_05250"
     CDS             2844..>3214
                     /locus_tag="VIJ44_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165340.1"
                     /translation="MRVDSFRFLPRSFRPLFEGARPLDGATDPVWAPFERRLSEATVM
                     LVTSAGLYRRGDQPSFDGDRERREPTWGDPSWRPIPRRTEQGELAMMHLHVNPVDVLA
                     DHEVALPLRALDRLVDEGIVG"
BASE COUNT          509 a         1058 c         1168 g          479 t
ORIGIN      
        1 ccgccgagcc gggggtctgg gtcacgatgg cgtctttaag cttcgccagg ctctgatcgc
       61 cgtcaccgcg aacaagatct acggtttcaa ctcgaagccc aaaggtcgca agggctggca
      121 gatcgtcgat caagtcgcgg aatgggaccg ccgcgacgtt cgggtctcct tcgacgatca
      181 gcgcatcacg cgtaccgtca ccctcgagat catctcgacg ggagtcctcc acgagctgga
      241 gatggtccag ctggccggag cgctgaacga tcccctcttc gcggcgctcg cggtgacgcc
      301 gtaaccctcc ggtctcgtcc ggcttcgagg gtcgagccga cgccgccctc gcggcggccg
      361 tggcggtcgg gctcgtgatg tcgaccgcgg tgccgagtac cgactcggcg cggtgctaga
      421 gccgctcgcg gagccgttcg atgtcggaga gatgatgcgt cgcatcgtgg accgcatgcc
      481 gtgcgatcca atgaacgtcg acgacctcac cgccgaccac gaccgtcgac tcccacgagg
      541 gttcgcccag ctggcggagc tgatcgtgga gctgctgcgc ctcctcgcgg agccctttga
      601 gtgccgcccg cacgtcgacg agacggggct cagggtcgaa ccgcgacgcc gggggctccc
      661 cgacatcggt gccggaggct tcgagcgccg tcgcgaggat gaaccgcatc ccgaacgtgg
      721 tctctcggac atggtcgagg tattcggcga tggaccacat ggtggcgacc ggtcgccgct
      781 ggagttcgct gtgccgaacg ccgtcgatcg cggaggcgaa accggcggga agctgggcga
      841 tcatggtcgt cgcgtcacga tccgtccaag ccgcgccatc gaagccgcat tgcggacagt
      901 cctctacgac caagctcgtg cacccctacg gtcgacgacc ggtctccgcg gcgagacgcg
      961 gcgctcgtcg gggtcggcgt cggctgcggt cacgtcgtcg acatccagct ctccacgagc
     1021 gggttccgtc cgtcgagttg gcctcgggag ccgtacgggc ggaaccggat gaaggcggac
     1081 tggtggtgga acggcttggc ccggtcggcg gtgatcgcgt ccgggtgcgc gggcttgggg
     1141 tgcccatagg cgtaggtcga gagcgcctgg gtggtctgcc acagcgagca cgtgccgaca
     1201 aagggcggac gcccgagccc ggttgcccaa gtgagcccgg gagcctcgac gaccgcacct
     1261 tcggctttgg cgctgacacg caagaagcgg atcgcttggc tgagacggag ccgtccgagc
     1321 gtcagcacgg cggccgggcc ctcatagtcg gtcgcgcgcg ccgtcggtac gttgtccggg
     1381 agtcccggcc aactgccgaa ggcacgcaat ggctcgagcc gcacgtgcca gcctcgagcc
     1441 agcttcgcgg cgagggggtc gtcggccaga aattcgtcga gggcgtcgtc gctgtcccag
     1501 aacgcgacaa gcccgatgcg tcccgggtcc ggggccgggc ggaacgagcc gcgcaacggc
     1561 gccgcgaggg ccgcgttggc atggcgcaac cccgtgatcg caccggggtc cggcggcttt
     1621 cggaggatcg acaatgccga ccgggcgccg acgtcggcga ggtgaacgga tgcgatcatc
     1681 atgggcgcct cccgccatat tccagcagga cacgcgccgc aggacgaatt caggtcgggc
     1741 cgatcggatg agccgggccg ggccgggccg tgctgctgat caccgacgac catgatcacg
     1801 cgggaacgga agaacggtcg gtgggggtgg gacgctcgga ggccgggccg cagacgggtc
     1861 caatcggtcg cgcgctcgtc ccggtgctgt gacaccgcgc cgggcggctg ggagcgttac
     1921 gaccgggaat gcttcttcga gcgtcgggtg ccggtggacc cttgcgcggc tttggtgccg
     1981 gccttcgtgg gcgggccccg aaggatccag gcgaagccga cggcgacgag gccaccgacg
     2041 attggtccga tgacgtaggc ccagaacgcg gtccagtggc caccgagcac cgcgggaccg
     2101 aacgagcggg ccgggttcat ggacgccccg ctgatcggag cggcccagag tcccgccagc
     2161 gcgatgtagg caccgacggc cagcgccgcg ttcgggccga tgtttcgccc cccggaggcc
     2221 gtcccgagga tgacgctgac gaggccaagc gtgagaacgg cttccaggag gaacaccttg
     2281 acgtcgctga tgccgtgcat gggaagcgtc gcgccgagat gcccgacgtt gccgaacatg
     2341 gcgcggagga acgcggcggc gacgacggcg ccgacgagtt gcattccgac gtagcccggc
     2401 actcgctgcc aggggaagtt gccgcgcagg gcgaacgaca gcgtcaccgc ggggttgaga
     2461 tgggcgcctc cgacggcgcc catgaagtag atgacggcca tcaccatcag gcccggtgcc
     2521 acgacctgtg ccgagagcga cacgcgcccg tggctcaccg cgttgacgac tggaccgccg
     2581 gccgcggcga gcaccaacaa gaacgttccg aacaactccg agaaccgccg gcgccaccgg
     2641 taccgggggt catcgaactg ttcgttggtg tgaggttcga acccgagggc cgacgtccat
     2701 ccggtcacaa ccggggttta ccccatcggt gggtgcgggg ctaccgcgac cgcacccgag
     2761 tgtgatgccc gggattcgtc tgcccggcat tcgtctgatg gttgctcgac gatggcgcct
     2821 ggcggcgtcc ataggctgtg ggcatgcgag tcgacagctt ccgtttcttg ccccgaagct
     2881 ttcgccccct gttcgaaggg gcccggccgc tcgacggggc gaccgacccc gtgtgggccc
     2941 ccttcgagcg acggctgtcg gaagccacgg tgatgctggt gacgtcggcg ggtctgtacc
     3001 ggcgcggcga ccagccgtcg ttcgacggtg atcgcgagcg ccgagaaccg acgtggggag
     3061 atccgagctg gcgtccgatc ccgcgccgga ccgagcaggg cgagctcgcc atgatgcact
     3121 tgcacgtcaa cccggtcgat gtgctcgccg accacgaggt ggcgctgccg ttgcgagccc
     3181 tggaccggct ggtcgacgag gggatcgtcg gaga
//
LOCUS       DATCCH010000272         2337 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_222421, whole genome shotgun sequence.
ACCESSION   DATCCH010000272 DATCCH010000000
VERSION     DATCCH010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222421"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2074
                     /locus_tag="VIJ44_05255"
     CDS             <1..2074
                     /locus_tag="VIJ44_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEY5165341.1"
                     /translation="RAPRRPTELRHGDDVRVDDWYWLRDRDDPAVRSLLEAENAYVEA
                     MLAPLGGVRDTVFEEIKTRVVETDVSARVRRGRFEYFTRTRAGLEYAMHCRRPVGAVA
                     PDPEAAPGQGDEEVLLDENALAAGTEYFALRGLALSPDQARLAYLVDRTGGERARLHV
                     RDLASGDDLPDEVPDTYYGLGWANDQRSIFYVRPDVAMRPFQVWCHVLGTAVATDRLV
                     FEESDERFYVSVRRARSGRVLFIDVESKVTTEAWLLDADDPDGTPRVVAPREEGVEYR
                     VEHHHRPSGEDRLFVVTNVDGAANFQLMVADTASPGREHWRTVVACRDEVRLDRVDAF
                     AHHVVLSERVGGMARLRALDLAGGDAVVEVGDDVATTWLGPSPEYDGTVVRVVSTSLV
                     TPTTDYDHDLATGTTTLVKQQRVEGYEPDRYETHRVWARADDATLVPISIVHRRGLAR
                     DGSGAMVLYGYGAYEISIDPAFSISRLSLLERGGAFAIAHVRGGGELGRHWYEDGKLD
                     HKRNTFSDFVTCARHLIAEGYTAPSRLVARGGSAGGLTMGAVLNQAPELWRAVVAEVP
                     FVDCLTTMLDPSLPLTITEFDEWGDPGADEQLYEYIRSYSPYDNVGPVAYPTVLVTAG
                     LNDPRVQYWEPAKWVQKLRATTTGTAATLLKTELDAGHHGRSGRYESWREEAFVVAFV
                     LDALGLTG"
     gene            complement(2064..>2337)
                     /locus_tag="VIJ44_05260"
     CDS             complement(2064..>2337)
                     /locus_tag="VIJ44_05260"
                     /inference="COORDINATES: protein motif:HMM:NF044908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEY5165342.1"
                     /translation="AAIAAVTDGWLPPVFGRTNEPLVVPTIDLTVHFRSALPHAGIPP
                     DGFVLAVFRTSVAADGFLDEDGEVWAPDGTLLAQSRQLAAILPLNR"
BASE COUNT          339 a          762 c          902 g          334 t
ORIGIN      
        1 gcgcgcgccg cgtcgcccga cggagctgcg tcacggcgac gacgtgcgcg tcgacgactg
       61 gtactggctg cgcgaccgcg acgatccggc ggtgcgctca ctgctcgagg cggagaacgc
      121 ctacgtcgag gcgatgctcg ccccgctggg cggcgtgcgc gacacggtgt tcgaggagat
      181 caagacccgg gtggtcgaga ccgatgtgag cgcgcgggtg cggcgcggcc ggttcgagta
      241 cttcacccgt acccgggcgg ggctcgagta cgcgatgcac tgccgccggc cggtcggcgc
      301 ggtggcaccg gacccggagg cggcgcccgg tcagggagac gaggaggtgc tcctcgacga
      361 gaacgccctg gcggccggga ccgagtactt cgcgttacgg gggttggcgc tgtcaccgga
      421 tcaggctcga ctggcatacc tggtggaccg aaccggcggt gaacgtgccc gactgcacgt
      481 gcgggacctg gcgtcgggcg acgacctgcc cgacgaggtt cccgacacgt actacgggct
      541 cgggtgggcc aacgaccagc gcagcatctt ctacgtccga cccgacgttg cgatgcgccc
      601 gttccaggtg tggtgccatg tcctcgggac cgcggtggcg acggaccggc tcgtgttcga
      661 ggagtccgac gagcgcttct acgtctcggt gcggcgcgcc cggtccggcc gggtgctgtt
      721 catcgacgtc gagtcgaagg tcacgaccga ggcgtggctc ctcgacgccg acgaccccga
      781 cggtacgcct cgggtcgtgg cgccccgcga ggaaggggtc gagtaccgcg tcgagcacca
      841 ccatcgcccg tcgggtgagg accggctctt cgtcgtcacc aacgtcgacg gcgccgcgaa
      901 cttccagctg atggtcgccg acactgcgtc accgggccgg gagcattggc ggacggtcgt
      961 cgcgtgccgg gacgaggtgc gtctcgaccg cgtcgacgcg ttcgcgcacc atgtggtgct
     1021 gtctgagcgg gtcggaggca tggcgcggct gcgggccttg gacctggccg gcggcgacgc
     1081 cgtggtggag gtcggtgacg acgtcgcgac gacgtggctc ggtccgagcc cggagtacga
     1141 cggaaccgtg gtgcgggtgg tgtcgacgtc gctggtgacg ccgaccaccg actatgacca
     1201 tgacctcgcg acggggacaa caacgctggt gaaacagcag cgggtcgaag ggtacgaacc
     1261 ggaccggtac gagacgcacc gcgtgtgggc ccgggccgac gacgccacgt tggtgccgat
     1321 ctcgatcgtg caccggcggg gtctggcccg ggacggttcg ggcgcgatgg tgctgtacgg
     1381 gtacggggcc tacgagatct ccatcgaccc ggcgttctcg atcagccggc tgagcctcct
     1441 cgagcgggga ggcgcgttcg cgatcgcgca tgtgcgcggc ggcggggagc tcggtcgcca
     1501 ctggtacgag gacggcaagc tggaccacaa gcgcaacacg ttcagcgact tcgtgacctg
     1561 cgcgcggcac ctcatcgccg aggggtacac cgcgccgagc cggctggtgg cccggggcgg
     1621 cagcgccggc gggttgacga tgggcgcggt gctgaaccag gcaccggagc tgtggcgggc
     1681 cgtcgtcgcc gaggtcccgt tcgtggactg cttgacgacg atgctcgacc cttcgctccc
     1741 gttgacgatc accgagttcg acgagtgggg cgaccccggc gccgacgagc agctctacga
     1801 gtacatccgc agctactccc cgtacgacaa cgtcgggccg gtggcgtacc cgacggtgtt
     1861 ggtcaccgcg gggctgaacg accctcgggt ccagtactgg gagcccgcca agtgggtgca
     1921 gaagctccgg gcgaccacga cggggaccgc ggcgacgctg ctcaagaccg agctcgacgc
     1981 cgggcaccac ggtcggagcg gccggtacga gtcgtggcgg gaggaagcgt tcgtggtggc
     2041 gttcgtcctc gacgcgctcg gccttaccgg ttgaggggca ggatcgccgc gagctggcgg
     2101 ctctgggcga ggagcgtccc gtcgggagcc cacacctcgc cgtcctcgtc gaggaacccg
     2161 tccgcggcga cgctggtgcg gaacaccgcg agcacgaacc cgtccggtgg gatgccggcg
     2221 tgcgggaggg cggagcggaa gtgcacggtg aggtcaatgg tcgggacgac cagcggctcg
     2281 ttggtgcgcc cgaacaccgg gggcagccag ccgtcggtca ccgcggcgat cgccgcg
//
LOCUS       DATCCH010000273         2255 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_429913, whole genome shotgun sequence.
ACCESSION   DATCCH010000273 DATCCH010000000
VERSION     DATCCH010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429913"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..858)
                     /locus_tag="VIJ44_05265"
     CDS             complement(121..858)
                     /locus_tag="VIJ44_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165343.1"
                     /translation="MTDRGLADRRAAWSLEFARPDGTGGFARLSRRGGQASYSAALVS
                     PTVGLVVVRDDGLATPRDSRLLVVRGDGLWAEFVEETRGEHWTAGLEAFGVRLDDPLD
                     AERGERGERLPVGFDLEWETGVGDYGEVHGDLLVATDRLVFEGTGRFEYESIRDEPLT
                     RESQRLSWQSQSDDGHTARDGDVHVDVDGRGLPFAARVGPLVFVVHAVVVVPVLGRVI
                     RALVVGEAGCGWLEWCQPQPGVRTDPS"
     gene            864..1889
                     /locus_tag="VIJ44_05270"
     CDS             864..1889
                     /locus_tag="VIJ44_05270"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY5165344.1"
                     /translation="MSVRVVSPVMPHTSSRPAAPATTRVIAADGVGVHTVEWSAPDAV
                     PGRRVLLLHGLGAHTLSWEPMAAPLADRLHARVIALDFVGFGRTRAPQRTASLETQRR
                     VLSALLAEEYGPALLIGNSMGATIGIGVAAAHPETVTGLVLVNPAVPHPRPGLAEWLR
                     IAWLAPLAVPAVGTGVVAWRARALGAERLVDTSLQASLTRAHELDPDLRRRMVELTAE
                     RLTWPEAPAAYAAAARSLVSYLARGVHRDLRRAAALRPTLLIHGSEDRLVPLEAAKHA
                     ARLHPLELAVLEGRGHAPQLEDPGAVIDVIDEWLGGPRFTDGRMGAWQASSTIPSRGS
                     SPTSATS"
     gene            1829..>2255
                     /locus_tag="VIJ44_05275"
     CDS             1829..>2255
                     /locus_tag="VIJ44_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165345.1"
                     /translation="MAGLLDDPVQGFVTDLSDVLADLRRRVTTPTDATALAIDAALEA
                     QAVAAAIIAADGHPSDAELRAFAEALSPWFESLRSATPTHLRTSAVIRQHRAFPITPS
                     PLFETIVAADTRDGTAYGWRYYDGAMRIAHAVCAIDPVPT"
BASE COUNT          332 a          903 c          721 g          299 t
ORIGIN      
        1 ccgcgagacc gatgccggcg atcgcctcga cctcatcgcg ggcctccatc ggcgcgcccg
       61 ccgccggcgc cacgaagatc cccaccagcg acccttcgaa catggcccca tcctgtcggt
      121 tcaggacggg tccgtacgaa ctccgggctg gggctggcac cactccagcc agccgcaccc
      181 cgcctcaccg accacgagcg cccggatgac ccgacccaag acgggcacga ccaccaccgc
      241 gtgcacgacg aagacgagcg ggccgacgcg cgccgcaaac ggaagccctc gaccgtcgac
      301 gtcgacgtgc acgtcgccgt cgcgtgcggt gtgaccgtcg tcggactggc tctgccatga
      361 cagccgctgc gactcgcgag tcaacggttc atcgcggatc gactcgtact cgaagcgccc
      421 cgtcccttcg aacaccaggc ggtccgtcgc gaccaggagg tcgccgtgca cttctccgta
      481 gtcccctaca ccggtttccc attccagatc gaagcccacc gggagacgct caccccgctc
      541 accccgctcg gcgtcgagcg gatcgtcgag acgaacgccg aacgcctcga gaccggcggt
      601 ccagtgctct cctctggtct cctcgacgaa ctcggcccag aggccgtcac cgcgaacgac
      661 gagcagccgg gagtcgcgcg gtgtggcgag cccgtcgtcg cgcaccacga cgaggcccac
      721 ggtcggggac accagggccg ccgagtacga cgcctgaccc cctcgccggc tcagccgcgc
      781 gaacccgccg gtcccatcgg gccgggcgaa ctcgagggac cacgccgcgc gccggtcggc
      841 aagaccccga tcggtcacca tcggtgagcg tacgcgtagt ctcaccggtc atgccgcaca
      901 cctcatctcg gcccgccgcg cccgcgacga cccgagtcat cgccgcggac ggcgtcgggg
      961 ttcacaccgt cgaatggtct gcgcctgatg cggtgcctgg ccggcgggtg ctgctcctgc
     1021 acggcctggg cgcgcacacc ctgtcttggg agccgatggc ggcaccgctc gccgaccgtc
     1081 ttcacgcccg ggtcatcgcc ctggacttcg tcgggttcgg acggacccga gctccgcagc
     1141 gaaccgcgag cctcgagacc caacgacggg tcctgagcgc cctgctcgca gaggagtacg
     1201 ggccggcgct cctgatcggc aactcgatgg gcgcgacgat cggcatcggc gtcgccgcgg
     1261 ctcatcccga aaccgtcacg ggcctcgtgc tggtgaaccc agccgtcccg cacccccggc
     1321 ccggcctcgc cgagtggctg cggatcgcct ggctcgcgcc cctggccgtc cccgcggtcg
     1381 gcaccggggt cgtggcctgg cgggcccgtg ccctcggcgc cgaacgtctc gttgacacct
     1441 cgctgcaagc gagcctcacc cgggcccacg agctcgatcc cgacctgcgc cgccgaatgg
     1501 tggaactcac tgccgagcgg ctcacctggc ccgaagcccc cgccgcctac gcggccgcgg
     1561 cccgatcgct cgtcagctac ctcgctcggg gcgtgcaccg tgacctgcgc cgcgccgccg
     1621 cgctccgacc gacgctgctc atccacggct ccgaggaccg cctcgtgccg ctggaggccg
     1681 ccaagcacgc ggcgcgcctc catccgcttg agcttgcggt gctggagggt cggggccacg
     1741 caccccagct cgaggacccc ggcgccgtga tcgacgtcat cgacgagtgg ctcggcggtc
     1801 cgcgcttcac ggatgggaga atgggcgcgt ggcaggcctc ctcgacgatc ccgtccaggg
     1861 gttcgtcacc gacctcagcg acgtcttagc cgacctccgc cgtcgcgtca cgaccccgac
     1921 cgacgcaacc gcgctggcga tcgacgccgc gctcgaggcc caagcggtcg ccgctgcgat
     1981 catcgcggcc gacggccacc cgagcgacgc ggagctgcgc gccttcgccg aggccctctc
     2041 gccatggttc gaatcgctgc gatccgcgac gccaacgcat ctgcggacca gcgcagtgat
     2101 ccgtcagcac cgagcgttcc ccatcacgcc gtcaccactg ttcgagacca tcgtcgccgc
     2161 cgacacgaga gacggcaccg cgtacgggtg gcggtactac gacggtgcga tgcgaatcgc
     2221 gcacgccgtc tgcgcgatcg acccggtccc gaccc
//
LOCUS       DATCCH010000274         2085 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_569578, whole genome shotgun sequence.
ACCESSION   DATCCH010000274 DATCCH010000000
VERSION     DATCCH010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569578"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1303
                     /gene="fusA"
                     /locus_tag="VIJ44_05280"
     CDS             <1..1303
                     /gene="fusA"
                     /locus_tag="VIJ44_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014144291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEY5165346.1"
                     /translation="NKGVQPMLDAVVRYLPSPLDVDAITGHAVGDEKVSIPRQPSEAE
                     PFSALAFKIMSDPHLGKLTYIRVYSGYLETGTQVLNSTKGKKERIGKIYKMHANNREE
                     IPRVGAGHIVAVMGLKDTTTGDTLSDAAHPVVLESMTFPAPVIHVAIEPKTKSDQEKL
                     GTAIQRLSEEDPTFQVRTDEETGQTIIAGMGELHLDVLVDRMRREFGVEANVGKPQVA
                     YRETITKAVDKVEYTHKKQTGGSGQYARVIIAIEPTSEDAEGAYEFVNKVSGGRIPRE
                     YIPSVDAGCQDAMEYGVVAGYPLVGIKVTLLDGQYHDVDSSELAFKIAGSMAFKDAAR
                     RAGPVIMEPVMAVEVTTPEDFMGEVIGDINSRRGHIQAMDERAGTRIVTALVPLSEMF
                     GYVGDLRSKTQGRASYSMQFDSYAQVPQNVALEIIAKARGE"
     gene            1377..>2085
                     /locus_tag="VIJ44_05285"
     CDS             1377..>2085
                     /locus_tag="VIJ44_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY5165347.1"
                     /translation="MAKAKFERTKPHVNIGTIGHIDHGKTTLTAAITKVLHDKYPAVN
                     PFTPFDQIDKAPEERQRGITISIAHVEYQTEARHYAHVDCPGHADYIKNMITGAAQMD
                     GAILVVAATDGPMPQTKEHVLLARQVGVPSIVVALNKCDMVDDEEILELVELEVRELL
                     SAYEYDGDNIPVVRVAAFPALNGDAKWGETILELMDAVDESIPTPVRETEKPFLMPIE
                     DVFTITGRGTVVTGRIER"
BASE COUNT          408 a          711 c          679 g          287 t
ORIGIN      
        1 gaacaagggc gtgcagccca tgctcgacgc ggtggtgcgc tacctgccgt cgccgctgga
       61 cgtcgacgcg atcaccgggc acgcggtcgg cgacgagaag gtctcgatcc cgcgccagcc
      121 gagcgaggcc gagccgttct ccgcgctcgc cttcaagatc atgagcgacc cgcacctcgg
      181 caagctcacc tacatccgcg tctactccgg ctacctcgag acggggaccc aggtgctgaa
      241 cagcaccaag gggaagaagg agcggatcgg caagatctac aagatgcacg ccaacaatcg
      301 cgaggagatc ccgcgcgtcg gcgccgggca catcgtggct gtgatgggtc tgaaggacac
      361 caccaccggc gacacgctgt ccgacgcggc gcacccggtc gtgctcgagt cgatgacgtt
      421 ccctgccccg gtcatccacg tggcgatcga gcccaagacc aagagcgacc aggagaagct
      481 gggcaccgcg atccagcgcc tgtccgagga ggacccgacc ttccaggtcc gcaccgacga
      541 ggagaccggc cagacgatca tcgccggcat gggcgagctg cacctggacg tgctggtcga
      601 ccgcatgcgg cgcgagttcg gcgtcgaggc gaacgtcggc aagcctcagg tcgcctaccg
      661 cgagacgatc accaaggccg tcgacaaggt cgagtacacc cacaagaagc agaccggtgg
      721 ctccggccag tacgcccggg tgatcatcgc gatcgagccc accagtgagg acgccgaggg
      781 cgcctacgag ttcgtcaaca aggtcagcgg cggccgcatc ccgcgggagt acatcccctc
      841 ggtcgacgcc ggttgccagg acgccatgga gtacggagtg gtcgccggat acccgctcgt
      901 cgggatcaag gtgaccctgc tcgacggcca gtaccacgac gtcgactcgt ccgagctcgc
      961 cttcaagatc gccggctcga tggccttcaa ggatgcggct cggcgagccg ggccggtcat
     1021 catggagccg gtcatggccg tggaggtgac caccccggag gacttcatgg gtgaggtcat
     1081 cggcgacatc aacagccgtc gtggccacat ccaggccatg gacgaacgtg cggggacccg
     1141 gatcgtcacg gccctcgtgc ccctctcgga gatgttcggc tatgtcggag acctccgcag
     1201 caagacccag ggtcgggcca gctactcgat gcagttcgac tcgtacgcgc aggtcccgca
     1261 gaatgtcgcc ctcgagatca ttgcgaaggc ccgcggagag tagttccccc gttccacccc
     1321 cgacccgtac ccgcacaccc cgcgggcgac gcaccgaata acttggagga cccccagtgg
     1381 cgaaggcgaa gttcgagcgg actaagccgc acgtcaacat cggcaccatc ggtcacatcg
     1441 accacggtaa gacgacgctg acggcggcca tcaccaaagt gctgcacgac aagtacccgg
     1501 ccgtgaaccc gttcacgccg ttcgaccaga tcgacaaggc acccgaggag cgtcagcggg
     1561 gcatcacgat ctcgatcgcg cacgtcgagt accagaccga ggcgcggcac tacgcccacg
     1621 tcgactgtcc tgggcacgcc gactacatca agaacatgat caccggtgcc gcccagatgg
     1681 acggcgcgat cctggtggtc gccgcgaccg acggccccat gccgcagacc aaggagcacg
     1741 tgctcctggc ccgccaggtg ggcgtcccgt cgatcgtggt cgcgctcaac aagtgcgaca
     1801 tggtcgacga cgaggagatc ctcgagctgg tcgagctcga ggtccgcgag ctgctcagcg
     1861 cgtacgagta cgacggggac aacatcccgg tcgtgcgcgt ggccgcgttc ccggcgctga
     1921 acggcgacgc gaagtggggc gagacgatcc tggagctgat ggacgcggtc gacgagtcga
     1981 tcccgacgcc ggtccgcgag accgagaagc ccttcctcat gccgatcgag gacgtcttca
     2041 cgatcaccgg gcgcggcacc gtcgtgaccg gccgtatcga gcgtg
//
LOCUS       DATCCH010000275         4156 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_113648, whole genome shotgun sequence.
ACCESSION   DATCCH010000275 DATCCH010000000
VERSION     DATCCH010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113648"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            472..876
                     /locus_tag="VIJ44_05290"
     CDS             472..876
                     /locus_tag="VIJ44_05290"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY5165348.1"
                     /translation="MTPPRGSVRRQVRIARTPDDVWAIVGDPGRIQDWFPGIVASTVD
                     GTTRVITTGSGTPMPEEIVTNDPLQRRFQYRITAPIFREHLSTLDVLDLGDGTSLVVY
                     SADANPATMALVIAGAAGNALEHLRDLLEGAR"
     gene            876..2411
                     /locus_tag="VIJ44_05295"
     CDS             876..2411
                     /locus_tag="VIJ44_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013424206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEY5165349.1"
                     /translation="MGRKILFVTTDQQRYDTLGCNGGALARTPVVDGLAANGVRYQRA
                     VPQAVVCMPSRATILTGQHPTTHGVWMNGVPLPVDAPSVADLLHHVGYRTALIGKAHF
                     EPYFDPFLRFTENALGASDAEPQNGTHRGFEHLEFATHGVMGSLHYSRWIQREHPEAA
                     GGFYAVIDGDLQVSGAGGGDTDAPQVKVNPIPRDWYHTDWVADRTISWLDSLDGDDDW
                     FCWMSFPDPHHPWDPPQSEVGRVDWRDVPLPAGYPESPDERAAILDAKPRHWRLWYDG
                     SLVSNYEVPATWVPATLTADQVREVNARNAVECELIDEALGRVLACIAARGWDDDVDV
                     VFTTDHGELQGDFGLLFKGPYHVDALMRLPLIWRPSASAGVEAAVVSGPVGLVDLAPT
                     FCDIAGVDTPAWMQGSSLPVTDGHPAREPVLTEWDSELFGVDVHLRTITRDRWVCTAY
                     RPGTVHDGTEGELYDLVEDPLQRSNLWDDPARRSLRSDLLADLWDRQPPGRDVRLELQ
                     APV"
     gene            2408..3652
                     /locus_tag="VIJ44_05300"
     CDS             2408..3652
                     /locus_tag="VIJ44_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165350.1"
                     /translation="MTPWRAEDGGPRRTQTVPGRLGVEPDERLEVTTRMAPATTMVVG
                     RERDELFLLRHTAGDDAVALVERIDPRTLEPLAESPSLTGGPAWPGGIGAHDNGSLYV
                     VFGNHAHRLDADLRPTASRALPRPRPYNSFVTLPDGHLVTKDFGGSRPGMVIPAEARE
                     PCELVVLDPDLEIVDRLVLAEPSIARLSADGDDIYVVADPNLLRVRWDGTRLAWDDTF
                     TSRYRTIEGQTFGWDCVIAAGAAWFLDDGDGSERFNGTLRGHGISTAPLHLVRVDLGT
                     SNVTMTQICGLPGGLVANPPIVDERRHLVVGYDSGNGVVAAFDLETLEPRWSRDQDHA
                     SHMLLYEGSGELVTGDHADVVLLDIATGAELARTDTRSGVQCVLFPSPGFERDFYLCS
                     MLAVTRVAVSNAGRRPGPFPRD"
     gene            complement(4027..>4156)
                     /locus_tag="VIJ44_05305"
     CDS             complement(4027..>4156)
                     /locus_tag="VIJ44_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012835266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEY5165351.1"
                     /translation="FASSRVIGWTAHILEQAADNRLIRPSAHYAGPPPPQPVPPAE"
BASE COUNT          633 a         1471 c         1419 g          633 t
ORIGIN      
        1 ggtgcgcgac caccactgtg ggtggtgctg catcgatgcg gccgtacggg gcgatcacat
       61 ctcgctgcca tagcctccgg gtggcgtgag cgacacggcc gtcgccggcg cgcatcggcg
      121 gccacggtaa cttctgggac ctaccgctaa caccggtcta ccggccggtg atcgctcagc
      181 gaacgagggc ttcatgccgt gtcagacctg gtgtcgagag tcactcgcgg tacgcatcgg
      241 ctcgatcatc ccgaacggtc ataccgatca gttggacgat gacgtcgtcg cggccaacga
      301 cgggccgtct cggcacgact ccaccaacgt tggtgccggg aggtccccgt cgttcgtggc
      361 gacgccgcat gatgatccga actgcaccgc cagcgggcgc gatgggcgca gttccgaaga
      421 gaaccacggt ggtgaggtcg cgcagctgat cgtcaccttc ggctcgcgga gatgacgccg
      481 ccgcgtggaa gcgtgcggcg ccaggtgcgc atcgcgcgaa cacccgacga cgtgtgggcg
      541 atcgtcggcg atccggggcg gatccaggat tggttccccg ggatcgtcgc gtcgacggtc
      601 gacggaacca cacgcgtgat caccactggc tcgggcaccc cgatgccgga ggagatcgtc
      661 accaacgacc cgctgcagcg ccggttccag taccgcatca ccgcgccgat cttccgcgag
      721 cacctctcga ccctcgacgt gctcgatctc ggcgacggga cgagcctcgt ggtctacagc
      781 gccgacgcca accccgcgac gatggcgctc gtcatcgcgg gcgcggccgg caacgcgctg
      841 gagcacctcc gcgacctcct ggaaggagcc cgctgatggg tcgcaagatc ctgttcgtca
      901 cgaccgacca gcagcgttac gacaccctcg gctgcaacgg tggggctctg gcccgcacgc
      961 ccgtcgtcga cgggctcgcg gcgaacggtg tccgctatca gcgggcggtg ccgcaggcgg
     1021 tcgtgtgcat gccgtcacgc gcgacgatcc tcacgggcca gcacccgacg acgcacggtg
     1081 tctggatgaa cggggtcccg ctcccggtcg acgcgccgtc cgtcgccgac ctcctccacc
     1141 acgtcggcta ccgcacggcg ctcatcggca aggcgcactt cgagccgtac ttcgacccgt
     1201 tcctgcgctt caccgagaac gcgctcgggg catcggacgc ggagccgcag aacggcactc
     1261 atcggggctt cgagcacctc gagttcgcga cccacggcgt gatgggctcg ctccactact
     1321 cccgctggat ccaacgcgag caccccgaag cggccggcgg tttctacgcc gtgatcgacg
     1381 gggacctgca ggtgagcggc gccggcggtg gcgacaccga cgcgccgcag gtgaaggtca
     1441 acccgatccc gcgggactgg taccacaccg actgggtcgc cgaccggacg atctcgtggc
     1501 tcgattccct cgacggggac gacgactggt tctgctggat gagcttccct gacccgcacc
     1561 acccgtggga cccgccgcaa tcggaggtcg gccgggtcga ctggcgagac gtcccgctcc
     1621 cggccggcta ccccgagtcg ccggacgaac gagcggcgat cctcgacgcc aaaccgcgtc
     1681 attggcgtct ctggtacgac gggtcgctcg tctccaacta cgaggtaccg gccacgtggg
     1741 tgccggcgac gctgaccgcg gatcaggtgc gggaggtgaa cgcgcgcaac gccgtcgagt
     1801 gcgagctgat cgacgaggcc ctcggacgcg tgctcgcctg catcgccgcg cggggctggg
     1861 acgacgatgt ggatgtcgtc ttcaccaccg accacggtga gctccagggc gacttcgggt
     1921 tgttgttcaa gggcccgtac cacgtggacg cgctcatgcg actgccgttg atctggcgcc
     1981 cgtcggcgtc cgccggcgtc gaggccgcgg tcgtgtccgg tcccgtcggg ctcgtcgacc
     2041 tcgcgccaac gttctgcgac atcgcggggg tcgacactcc ggcgtggatg caggggtcgt
     2101 cgttgccggt cacggacggc cacccggctc gtgagcccgt cctgaccgag tgggatagcg
     2161 agctgttcgg cgtcgacgtc cacctgcgga ccatcacccg tgatcgatgg gtctgtaccg
     2221 cctaccggcc cggcaccgtc cacgacggca cggagggcga gctctacgac ctcgtggagg
     2281 acccgctgca gcggagcaac ctgtgggacg atccggcgcg gcggtcgctg cggtccgatc
     2341 tcttggctga cctgtgggac cgtcagcctc ccggccggga cgtcaggctc gaactgcagg
     2401 cgccggtgtg acgccatggc gagccgagga cggcggccca cgccggaccc agacggtccc
     2461 cggccggctc ggcgtcgagc ccgacgagcg gctcgaggtc accacgcgga tggcgccggc
     2521 cacgaccatg gtcgtcggcc gagagcgaga cgagctgttc ctcctgcgcc acaccgccgg
     2581 cgacgatgcc gtcgccctcg tcgagcgcat cgacccgcgc acgctcgagc ccctcgctga
     2641 atctccgagc ctgacgggcg ggccggcatg gcccgggggg atcggcgccc acgacaacgg
     2701 atcgctctac gtcgtgttcg gcaaccatgc ccaccggctc gacgccgacc tgcgacccac
     2761 ggcgtcgcgc gccttgcccc gcccgcggcc ctacaacagc ttcgtgacgc tccctgacgg
     2821 ccatctcgtc accaaggatt tcggcggctc ccgcccgggg atggtgatcc cagccgaggc
     2881 gcgtgagccc tgcgagctgg tggtcctcga cccggacctc gagatcgtgg atcggctggt
     2941 cctcgccgag ccgtcgatcg cgcggctctc agccgatggc gacgacatct acgtcgtggc
     3001 tgatcccaac ctgctccggg tccgctggga cgggacacgt ctcgcctggg acgacacctt
     3061 tacctcccgc taccgaacga tcgaggggca gacgttcggc tgggattgcg tcatcgccgc
     3121 cggcgcggcc tggttcctgg atgacggtga cggcagcgag cgcttcaacg gcacccttcg
     3181 tggccacggc atctcgaccg ctccgctcca tctcgtccgg gtcgacctcg gcaccagcaa
     3241 cgtcacgatg acccagatct gcgggcttcc cggcgggctc gtcgcgaacc cgcccatcgt
     3301 cgacgaacgg cgccacctcg tcgtcggata cgacagcggc aacggcgtcg tcgcggcgtt
     3361 cgacctggaa accctcgagc cgcgctggag ccgtgaccag gaccatgcca gccacatgct
     3421 cctctacgag ggctccggcg aactcgtgac cggcgaccac gccgatgtgg tcctcctcga
     3481 catcgcgacc ggcgccgaac tcgcgcgcac cgacactaga agcggtgtgc aatgcgtcct
     3541 gttccccagc cccgggttcg aacgggactt ctacctctgc tcgatgctgg cggtcacccg
     3601 cgtcgccgtc tcgaacgcgg gaaggagacc gggacccttc ccgagggact gagcgaggta
     3661 tcggcggcgg cggagaagct ggcttcctcg ccgcggcggg aacggctgtg ccccccgacg
     3721 ggtggtcgac caccaggtgg tcgcgccacg gagtcaacca cgccccgtcg gcaggttcga
     3781 gcagcaaaat cgctcggctt cccgagttgg acggctcagc gcgggatccg gttgagggtg
     3841 tcgacggcgc cagtgagatc accgagggtc gagaagtcga cgttgatgaa gttgggtcgg
     3901 cggccaccgg tgctcaacag ctggtctcgg acctcgagtg caacgcagag cgcaaacggc
     3961 gcgccgagcg gccgtcgcgc ttcgacatga atccagcgta cgggatactg acgctgctga
     4021 tcctggctac tcggccggcg gcaccggctg tggtggggga ggaccggcgt agtgggcgct
     4081 gggtcggatg agccggttgt ccgcggcctg ctcgaggatg tgcgcggtcc agccgatcac
     4141 ccggctggac gcgaac
//
LOCUS       DATCCH010000276         2395 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_279452, whole genome shotgun sequence.
ACCESSION   DATCCH010000276 DATCCH010000000
VERSION     DATCCH010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279452"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1171)
                     /locus_tag="VIJ44_05310"
     CDS             complement(<1..1171)
                     /locus_tag="VIJ44_05310"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HEY5165352.1"
                     /translation="MALADLPAAGTWTNWVGNQSFTPARFARPSSSEEVAALVGEAAA
                     QGMGVRVVGAGHSFTPVVQTDGLLLDLSALAGVVDADPVRKRARARAGTPIHGFYEPL
                     WEHGLALRNQGDIDDQHIAGAVATATHGSGIGNSCFSGVVRGVSLVTPDGSIRHIGED
                     EPRLLRAAQVSVGTLGVMTELEIEVAEAYRLSEQVGVWPWQEVMDRWDELVHGHRHFG
                     FFWMPSEESAALYNIEGHGAMTDRCYVKIYDEATPDQPDSAEPGRRTDRCYRIYPMVY
                     DPNFHELEYFVPLERGPEALGAMRELMLASLPDSVYPLEVRTVGPDDAFLSPNYKTAT
                     TVISVSGTPGTDYWDYLRSVDALLAQFDARVHWGKLHFLDSERLHSLYPEADEFIA"
     gene            1210..>2395
                     /locus_tag="VIJ44_05315"
     CDS             1210..>2395
                     /locus_tag="VIJ44_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165353.1"
                     /translation="MIGVSRVWWVLAVVATVLACAVATAGAAPLRWSEAHAVDAAQGV
                     TLGSLQCPSATGCVALDNRGRYVTYKPSAPGRPVPVALGAATEPIALSCPSATQCTAL
                     DLGGDEVTFDPTKSSPPAAVNVDPAADPGGAEAANDIACASTTQCVIVDGKGNVVVFD
                     PATPSAAGTTSLNPGEDFGLESVTCVSATQCTAVSETKAVTFNPAAPAGATPVVLGSK
                     LLMTIVACPTATQCTAVDGIAGEALTFNPQAPAPTTPVAVDSESDNQLLALVCPSATQ
                     CTTTSQGGRTITFDPQTGQGRAFTVDPSPGGDAIGQETGVTAVACVSTSSCVAVDGAG
                     RTMSFDPTASGTQPPVQIDSGSELFGVACATATQCTAIDTTHELTFNPFKPGTPKLRK
                     LFT"
BASE COUNT          356 a          846 c          849 g          344 t
ORIGIN      
        1 cggcgatgaa ctcgtcggcc tccggataga gggagtgcag gcgctctgag tccagaaagt
       61 gcagtttgcc ccagtgcacg cgggcatcga actgggcgag cagggcgtcg accgagcgca
      121 ggtagtccca gtagtcggtg ccgggcgtgc cggacaccga gatgacggtg gtcgccgtct
      181 tgtaattggg cgacagaaac gcgtcgtcgg gtcccacggt ccggacctcc agtgggtaca
      241 cggagtccgg caggctggcg agcatcagct cgcgcatcgc ccccagcgcc tcggggccgc
      301 gctccagggg cacgaagtac tcgagctcgt ggaagttggg gtcatagacc atgggataga
      361 tgcggtagca gcggtcggtc cgccggcccg gctcggcgct gtcgggctgg tcgggcgtcg
      421 cttcgtcgta gatcttcacg tagcagcggt cggtcatcgc accgtggccc tcgatgttgt
      481 agagcgccgc ggactcctcg ctcggcatcc agaagaagcc gaagtgccgg tggccgtgga
      541 ccagttcgtc ccagcggtcc atcacctcct gccacggcca gacgccgacc tgctccgaga
      601 gccggtaggc ctcggcgacc tcgatctcga gctcggtcat cacgccgagc gtgcccaccg
      661 acacctgggc ggcacgcagc aggcgcggct cgtcttcgcc gatgtgccgg atcgaaccgt
      721 cgggcgtcac cagcgagaca ccccggacca cccccgagaa gcaggagttg ccgatcccgg
      781 agccatgggt ggcggtcgcc accgcgccgg cgatgtgctg gtcgtcgata tcgccctggt
      841 tgcgcagcgc cagcccgtgc tcccatagcg gctcgtagaa gccgtggatc ggggttccgg
      901 cccgggcgcg cgcccgcttg cgcaccgggt cggcgtcaac cacgccggca agcgcgctca
      961 gatcgaggag cagcccgtcg gtctgcacga ccggcgtaaa cgagtggccg gcgccgacga
     1021 cgcgcacccc catcccctgt gcggcggcct cgccgacgag cgccgcgacc tcctccgagc
     1081 tcgacgggcg ggcgaatcgc gctggcgtga acgactggtt gccgacccag ttggtccacg
     1141 tgccggcggc gggaaggtcg gcgagcgcca ttgcggcagc ctaacccccc ggatagggtc
     1201 ctgtcacgga tgatcggcgt gtcgcgcgtg tggtgggtcc tcgccgtggt ggcgaccgtt
     1261 ttggcgtgcg cggtggcgac cgccggcgcg gccccgttgc gctggtctga ggcacacgcg
     1321 gtcgatgccg cccagggcgt gacgctcgga tcgctgcagt gcccgtccgc gacggggtgc
     1381 gtggcgctgg acaaccgcgg ccgctacgtc acctacaagc cgagcgcgcc gggtcggccg
     1441 gtgccggtcg cgctcggggc ggccaccgag ccgatcgccc tgtcgtgccc gtcggcgacg
     1501 cagtgcacgg cgctcgacct cggcggcgat gaggtgacgt tcgatccgac caagagcagc
     1561 ccgccggcgg ccgtcaacgt cgaccccgcg gcggatcccg gcggagccga ggcagccaac
     1621 gacatcgcgt gcgcctcgac cacgcaatgc gtgatcgtcg acggcaaggg caatgtcgtt
     1681 gtgttcgatc cggccacccc gagcgccgcc ggcacgacct cgctcaaccc aggcgaggac
     1741 ttcgggctcg agtcggtcac gtgcgtgtcg gccacccagt gcacggcggt cagtgagacc
     1801 aaggcggtca cgttcaaccc ggccgcgcct gccggcgcca cacccgttgt cctcggctcc
     1861 aagctgctga tgacgatcgt cgcctgtccg acggccaccc agtgcacggc ggtcgacggc
     1921 atcgccggcg aggcgctgac gttcaacccg caggcgccgg cgccgaccac cccggtggcg
     1981 gtggacagcg agagcgacaa ccagctgctc gcccttgtgt gtccctcggc gacgcagtgc
     2041 acgacgacct cccagggcgg acgcacgatc acattcgatc cgcagaccgg ccagggccga
     2101 gcgttcacgg tcgacccgtc gcccggtggc gacgcgatcg gtcaggagac cggcgtgacc
     2161 gccgtcgcct gcgtctcgac ctcgagctgc gtggcggtcg acggtgccgg ccggacgatg
     2221 tcgtttgacc cgacagcgtc gggcacccag ccgccggtgc agatcgactc gggcagcgag
     2281 ctgttcggcg tcgcctgcgc gacggccacc cagtgcacgg cgattgacac gacgcacgag
     2341 ctgacgttca acccgttcaa gccgggcacg ccgaagctgc gcaagctgtt cacgc
//
LOCUS       DATCCH010000277         2012 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_56295, whole genome shotgun sequence.
ACCESSION   DATCCH010000277 DATCCH010000000
VERSION     DATCCH010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56295"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..1210
                     /locus_tag="VIJ44_05320"
     CDS             149..1210
                     /locus_tag="VIJ44_05320"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020658417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEY5165354.1"
                     /translation="MQLPVLPPVEPMLAKLARELPSGDGWSYEPKWDGFRCVVFRDLD
                     EIELGSRNGRPLNRYFPEILDPLRAALPERVVLDGELVIATPDGLDFDLLSLRIHPAA
                     SRVAKLAGETPASFVAFDILAVAGDDVRDEGFAARRARLEQSVTEGPLIHRTPATTDR
                     DVAAKWFDDFEGAGLDGVVAKALDSPYREGERTMVKVKHQRTADCVVAGYRTHKDGRG
                     VGSLLLGLYDDSGSLHHVGVASSFAAPLRAQLVDELAPYRDDALHNHPWRDWGGSDAT
                     STRMPGSPSRWNATKDLSWEPLRPELVAEVAYEHLQGDRFRHTARFARWRPDREPRSC
                     TYSQLDAPVPFELREVFGL"
     gene            complement(1233..1991)
                     /locus_tag="VIJ44_05325"
     CDS             complement(1233..1991)
                     /locus_tag="VIJ44_05325"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase small subunit domain-containing
                     protein"
                     /protein_id="HEY5165355.1"
                     /translation="MRVTCPSGRHADELCPVDVAHLIWAVNLGCLDLNPWPVRRRDVD
                     HPDELRVDLDPQPGVDFDVVRRVAGLIHEVLEEHHLVGFPKTSGSRGIHVNVRVEPRW
                     DFLGVRRAALALARAVERRAPDLATSKWWKEQRGDRVFIDYNQNARDRTVASVYSVRA
                     NPEGRVSCPLEWDEVADVDPRDLTLATVPARYAERGDLGAGIDDAVCSLDELLALADR
                     DETEGLGDEPWPPHFPKQAGEPRRVQPSRARKRP"
BASE COUNT          295 a          800 c          642 g          275 t
ORIGIN      
        1 ccgacgcttc gtgctgggca gcgtcccgaa ccacgtgtcg caccacgccc cgtgcagcgt
       61 gttcatcgtc aacaccgccg accccgccga ctgaccgtcg cgcgcccgac gaccgcggcg
      121 gacgggtcgg tcgtggcagg gtgctgagat gcagctgccc gtcctgccgc cggtcgaacc
      181 gatgctcgcc aagctggccc gcgagctccc cagcggcgac ggctggtcgt acgagccgaa
      241 gtgggacggg ttccgctgcg tcgtgttccg cgacctcgac gagatcgagc tcggcagccg
      301 caacggacgt cccctcaacc ggtacttccc cgagatcctc gaccccctcc gcgccgcgct
      361 gcccgaacgc gtggtgctcg acggcgagct cgtcatcgcg acccccgacg gcctcgactt
      421 cgacctcctc tccttgcgta tccatcccgc ggccagccgg gtcgcgaagc tcgccggcga
      481 gaccccggcc agcttcgtcg ccttcgacat cctcgccgtc gccggtgacg acgtgcgcga
      541 cgagggcttc gcggcccgcc gggcccggct cgagcagagc gtcaccgaag ggccgctcat
      601 tcatcgcacc ccggcaacga ccgaccgcga cgtggcggcg aaatggttcg acgacttcga
      661 gggcgccggg ctcgacggtg tcgtcgccaa ggcgctcgac agcccctacc gcgaaggcga
      721 acgcacgatg gtcaaggtga agcaccaacg aaccgccgac tgcgtcgtcg ccgggtaccg
      781 aacccacaag gacggccggg gggtcggctc gctgctcctc ggcctctacg acgacagcgg
      841 ctcgctccac cacgtcggtg tcgccagcag cttcgccgcg ccgctgcgcg cccagctcgt
      901 cgacgagctc gcgccgtacc gcgacgacgc gctgcacaac cacccgtggc gcgactgggg
      961 gggcagcgac gcgacctcga cccgcatgcc cggatcgccg agccgttgga acgcgacgaa
     1021 agacctctcg tgggagcccc tgcgccccga gctcgtcgct gaggtcgcct acgagcacct
     1081 ccaaggcgac cggttccgtc acaccgcccg cttcgcccgc tggcgccccg accgtgaacc
     1141 ccgctcgtgc acctacagcc agctcgacgc ccccgtgccg ttcgagctgc gcgaggtctt
     1201 cggcctctag tccactcgtg cgttggtcag ggtcagggac gtttgcgggc ccggctgggc
     1261 tgcacccgcc gaggctcccc ggcctgtttg gggaaatggg gcggccacgg ctcgtcgccg
     1321 agcccctcgg tctcgtcgcg gtcggctagg gccagcagct cgtcgaggga gcacaccgcg
     1381 tcgtcgatcc ccgcgcccag gtcaccccgc tccgcgtagc gggcggggac cgtcgccaaa
     1441 gtcagatccc ggggatcgac atcggcgacc tcgtcccact cgagcgggca cgacacccgc
     1501 ccctccgggt tggcccgcac cgagtacacc gacgccaccg tccggtcccg ggcgttctgg
     1561 ttgtagtcga tgaacacccg gtcgcctcgc tgctccttcc accacttcga cgtcgccagg
     1621 tccggcgccc gccgctccac ggcgcgggcc agcgccagcg ccgcccgacg cacccccaag
     1681 aagtcccaac gcggctcgac ccgcacgttc acatggatgc cccgcgaccc cgacgtcttc
     1741 gggaacccga ccaggtgatg ctcctcgagg acctcatgga tcaaccccgc caccctccgg
     1801 acgacgtcga agtccacccc cggctggggg tccaggtcga ctcgcagctc gtcgggatgg
     1861 tcgacgtcgc gacgccgcac cggccacgga ttcaggtcca gacagcccag attcaccgcc
     1921 cagatcaggt gcgcgacgtc gaccgggcac aactcgtccg cgtggcgccc ggacgggcac
     1981 gtgacccgca ccgtctacag ccagtcgggg cg
//
LOCUS       DATCCH010000278         3173 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_528941, whole genome shotgun sequence.
ACCESSION   DATCCH010000278 DATCCH010000000
VERSION     DATCCH010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528941"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VIJ44_05330"
     CDS             <1..238
                     /locus_tag="VIJ44_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HEY5165356.1"
                     /translation="VIAAAGVPGILQPDHLSPGVVVVGGGVRYEGRKLLPDVDERCEK
                     VAAAITPRVGGVGPTTIAMLFRNMVEAAERSRAR"
     gene            235..456
                     /locus_tag="VIJ44_05335"
     CDS             235..456
                     /locus_tag="VIJ44_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165357.1"
                     /translation="MSSEPTPAPIPDSECPLCATMVAGQARRCPECGWSLAGVDGRPG
                     PYSKAALWWTGSGFLVVYLITLAIVALTH"
     gene            490..1692
                     /gene="icd"
                     /locus_tag="VIJ44_05340"
     CDS             490..1692
                     /gene="icd"
                     /locus_tag="VIJ44_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEY5165358.1"
                     /translation="MPEKITMVDGTLTVPDEPIIPFIEGDGTGVDIWPAAKHVLDAAI
                     DKAYRGGRRVAWTEVLAGEKAYRETGDWLPEDTVASFREHLIGIKGPLTTPVGGGIRS
                     LNVALRQILDLYVCLRPVRWYPGVPSPVKRPELIDMVIFRENTEDIYAGLEVEEGTPE
                     AKKLIETLKRDLGWDIRSDSGIGIKPISETGSKRLIRAAIEYALKHHRPSVTLVHKGN
                     IQKFTEGAFRNWGYELTREEFAGVAVGWDDCNGDPGDKVLVKDNIADITLQQVLTRPE
                     DFDVIATTNLNGDYLSDALAAQVGGIGIAPGGNINYVTGHGIFEATHGTAPKYAGQDK
                     VNPASLLLSGVMMLEHLGWNEPAELITKAFEATIADKVVTYDFARLMEGAQEVKTSQF
                     ADAVVNHM"
     gene            1772..2575
                     /locus_tag="VIJ44_05345"
     CDS             1772..2575
                     /locus_tag="VIJ44_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEY5165359.1"
                     /translation="MLLAELNIRHSRRHQPTRRVALGDTYLPTSGAAFGAVLLAAVVA
                     EHLDELDEDQRDLLPRLLDDARQGLGVPRIALRYRLQTDTHGLDRSRHRIVSESLGSN
                     DRPASLVLEFDEHAAGAPQLIGAVMAAAALPSTARRVALRAVEEAVIRPALPEGLVVR
                     RLHEGVPGLQPFAPQMGAVEGEIESLWRGVPAERRWAMEVLGLRAGMELGRADVQARF
                     RRLVRLAHPDHGAARDGAAERLAELREARELLLSVAASMPGSAGKQRTG"
     gene            2587..>3173
                     /locus_tag="VIJ44_05350"
     CDS             2587..>3173
                     /locus_tag="VIJ44_05350"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEY5165360.1"
                     /translation="MPSAPVHVAVTGAAGQIGYALVHRIAAGELLGHEQPVVLRLLEI
                     EPAMKALEGLVMELEDGAHPLLEGIEATSDVNHAFDGASWCLLVGSIPRKAGMERREL
                     LSVNGGIFRPQGRAINAHAASDVRVLVVGNPCNTNCLIARSNAPDIPDDRWFAMTRLD
                     ENRAKSQLAKKAGVPTRAVTNMAIWGNHSATQFPD"
BASE COUNT          525 a         1058 c         1081 g          509 t
ORIGIN      
        1 cgtcatcgcc gccgccgggg tccccggcat cctccagcct gaccacctct ctccgggggt
       61 ggtcgtcgtc ggcggggggg tccgctacga gggccgcaag ctcctccccg acgtcgacga
      121 gcgctgtgag aaggttgcgg cggcgatcac cccccgggtg ggtggcgtgg ggccgaccac
      181 aatcgccatg ctgttccgca acatggtgga ggccgccgag aggagcaggg cgcggtgagt
      241 agcgaaccaa cgccggcccc gatcccggac tcggagtgcc cgctctgcgc gacgatggtg
      301 gccggccagg cgagacggtg tcccgagtgt ggctggtcgc tggccggcgt cgacggccgt
      361 cccggtccgt actcgaaagc cgctctgtgg tggactggca gcggtttcct tgtcgtctat
      421 ctcatcacgt tggcgatcgt cgcgttgacg cactgacgcg cgcttacccc cgaagcgagc
      481 aggagtccga tgcccgaaaa gatcaccatg gtggacggga cgctgaccgt cccggacgag
      541 ccgatcatcc cgttcatcga aggtgacggc acgggtgtcg acatctggcc cgcggcgaag
      601 cacgtcctcg acgccgcgat cgataaggcg taccggggtg gccggcgcgt cgcgtggacc
      661 gaagtcctcg ccggcgagaa ggcctaccgt gagacgggcg actggctgcc cgaggacact
      721 gttgcgtcgt tccgcgagca tttgatcggc atcaaggggc cgctcacgac cccggtgggt
      781 gggggaatcc ggtcgctcaa cgtcgcgttg cgccagatcc tcgacctgta cgtctgtctc
      841 cggccggtcc ggtggtatcc gggtgtcccg tcgccggtga agcgccccga actcatcgac
      901 atggtcatct tccgcgagaa caccgaggac atttacgccg gcctcgaggt cgaggaagga
      961 accccggagg ctaagaagct catcgagacc ctcaagcgcg acctcggttg ggacattcgc
     1021 tcggattccg ggatcggcat caaacccatc tcggaaaccg gctcgaagcg cctcatccgc
     1081 gccgcgatcg aatacgcgct caagcatcac cgcccgagcg tgaccctcgt ccacaagggc
     1141 aacattcaga agttcaccga aggcgcgttc cggaactggg gctacgagct gacgcgagag
     1201 gaattcgctg gtgtcgccgt cggatgggac gactgcaacg gcgatcccgg cgacaaggtg
     1261 ctcgtcaagg acaacatcgc cgacatcacg ctgcagcagg ttctgacccg acccgaggac
     1321 tttgacgtga tcgcaaccac caacctcaac ggcgattacc tctccgatgc gctcgcggcc
     1381 caggtcggag gaatcgggat cgcgcccggt ggcaacatca actacgtcac cgggcacggc
     1441 atctttgagg cgacacacgg gactgccccg aagtacgcgg gtcaggacaa ggtcaacccg
     1501 gcttcgctgc tgctctcggg agtcatgatg ctcgaacacc tcgggtggaa cgagccggcg
     1561 gagctcatca ccaaggcgtt cgaggcgacc atcgccgaca aggtcgtgac ctacgacttc
     1621 gctcggctga tggaaggcgc acaagaggtc aagacctcgc agttcgccga cgccgtcgtc
     1681 aaccacatgt gagctcgctc gcgatgccgg ctcggcggcc acgctcatcc acgcggcgga
     1741 ccgatgccga atggttgggt aggaggcgcg agtgctgctc gccgagctga acatccgcca
     1801 cagtcgccgt caccagccaa cccgacgagt cgcgctcggc gacacctatt tgccgacgag
     1861 cggtgcggcg ttcggggcag tcctactggc cgcggtggtg gccgagcatc tcgacgagct
     1921 cgacgaggac cagcgcgacc tcctacctcg cctcctcgac gatgcgcgcc agggcctcgg
     1981 cgtaccccgt atcgctctcc gatatcggct ccagacggac acgcacggcc tggaccgctc
     2041 ccgtcatcgc atcgtcagcg agtcgctcgg ctcgaacgac cgtccggcgt cgttggtcct
     2101 cgaatttgat gagcacgccg ccggagcgcc ccagctgatc ggtgccgtca tggccgcggc
     2161 ggcgctgcca tcgacggccc gccgggtcgc gctacgggcg gtcgaggagg cggtcatccg
     2221 tccggcgctc ccggagggcc ttgtggtgcg ccgcctgcac gaaggcgtcc ccggcctgca
     2281 gcccttcgcg cctcagatgg gggccgtgga gggtgagatc gagtccctgt ggcggggggt
     2341 gccggccgag cggcgctggg cgatggaagt cctcggtctg cgagccggca tggagctcgg
     2401 tcgggcggat gtacaggccc gctttcggcg actggttcgc ctggctcacc ctgatcacgg
     2461 cgccgcgcgc gacggtgccg ccgaacgact cgccgagctc cgcgaggcgc gggagctcct
     2521 cttgtccgtg gcggcgtcga tgccaggttc ggccgggaag cagcgcaccg ggtaggttcc
     2581 cgagccatgc cgagcgcacc agtccacgtc gccgtcaccg gggccgccgg gcagatcggg
     2641 tacgccttgg ttcatcgcat cgcggcgggt gagctgctgg gccacgagca gccggtggtg
     2701 ctgcgcctcc tcgagatcga accggcgatg aaggcgctcg agggattggt catggagctc
     2761 gaggatggcg cccatccgct gctcgaaggc atcgaggcga cctcggatgt caaccacgcg
     2821 ttcgacggcg cttcgtggtg cttgttggtc gggtcgatcc cccggaaggc ggggatggaa
     2881 cgacgcgagc tcttgtcggt caatggtggg atcttccgac cgcaggggcg agcaatcaac
     2941 gcgcatgccg cctccgacgt gcgggtcctc gtcgtcggga acccgtgcaa cacgaactgc
     3001 ctgatcgccc ggtcgaacgc gcccgatatt cccgacgatc gatggtttgc catgactcgt
     3061 ctcgacgaga accgggccaa gtcgcagctg gccaagaagg ccggcgtccc gacacgcgcg
     3121 gtcacaaata tggctatctg gggtaaccat tccgcgacgc agttcccgga ctg
//
LOCUS       DATCCH010000279         4140 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_139648, whole genome shotgun sequence.
ACCESSION   DATCCH010000279 DATCCH010000000
VERSION     DATCCH010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4140
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139648"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="VIJ44_05355"
     CDS             <1..517
                     /locus_tag="VIJ44_05355"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5165361.1"
                     /translation="GTGAIDAQPLEEVDRILAVNLRGVILGLRAAVPTLRAGGGGSIV
                     VTASVSGLFGDPGLFAYNASKAGAVNVVRAAALDLGRDGIRVNAVCPGPIRGTGMTGP
                     LERRAGDLYAEMRSHLPLGRFGEPAEVAAVAAFLLSDDASYVTGVALPVDGGVTAGTG
                     QFRAVYPAESG"
     gene            complement(566..937)
                     /locus_tag="VIJ44_05360"
     CDS             complement(566..937)
                     /locus_tag="VIJ44_05360"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="limonene-1,2-epoxide hydrolase family protein"
                     /protein_id="HEY5165362.1"
                     /translation="MGSGEVVAALIKANEARDLDAIVALMTDDIAYENVGMGVCNGAD
                     EVRALLGPFYAGTERVAWEVLAQVEQGDVVMNERVDRFFLPGGKQAEARVAGYFEVRD
                     GKVSRWRDYFDTTALMTQLTG"
     gene            1003..2634
                     /locus_tag="VIJ44_05365"
     CDS             1003..2634
                     /locus_tag="VIJ44_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEY5165363.1"
                     /translation="MSDARERWQSAFDATPSRDADFETMSGMPVEPVYGPDESLPGAW
                     PYTRGPYASMYRSKLWTMRMFAGFGTAIDTNRRFRELLAAGGSGLSTAFDLPTLMGRD
                     SDDELALGEVGKGGVSVDSLADVVDLYAGIDLGHVTTSMTINAPAPILLAMYVAAAEA
                     TGVARRDLGGTIQNDILKEYQAQKEFVFPPRPSMRLVNDTIRFCEAEMPRWHPVSISG
                     YHIREAGSTAVQELAFTLANGFAYVEQAASSGLAVDEFAPRLSFFFNAHIDFFEEIAK
                     YRAARRIWARWMRDRYGAHEERSMQLRFHAQTAGVSLTAQQPEVNLVRTAVEALAAVL
                     GGTQSLHTNSMDETMALPTDKSARLALRTQQVLAFENRVANVADPLGGSWYVEALTDE
                     MERRAEAIFEQLEGFGDGSMLEGVLRAVESGWFQRNIADSAYELERKTNSGHNVVVGV
                     TDFFEGNEEAPPELLRIGPEFEERQLKRLAALRADRDDGRVDAVLARVRADAAEPTTN
                     VLPALIEAVQGDATLGEIVNAFADVFGRYTEAPMI"
     gene            2688..3254
                     /locus_tag="VIJ44_05370"
     CDS             2688..3254
                     /locus_tag="VIJ44_05370"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018385191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY5165364.1"
                     /translation="MSDDEGLALHDAGLAAAAMGPLLEVGTYCGKSAIYLGAAARERD
                     TVLFTVDHHRGSEENQAGWEWHDERLVDPESGRLDTLPWFRRTIAAAALEATVVAVVG
                     DSPTVARHWRTPLGLVFIDGGHAFNVAAADYLGWAPQVAPGGLLVFHDVFEDPADGGQ
                     APFEVWRRAVSDGFTPVATTGSLRVLRR"
     gene            complement(3258..>4140)
                     /locus_tag="VIJ44_05375"
     CDS             complement(3258..>4140)
                     /locus_tag="VIJ44_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HEY5165365.1"
                     /translation="GCWHAYYVGRDVKDWALDTNVTCYSANGVWHHYLSTGDTGFLEE
                     FWSVVERGIDFALSHQAETGEIAWRGDDPANGALLTGSSSIHASLRCAIAIAERLGHE
                     RPDWELSLGALAIAVAHRPDHFLEKARWAMDWYYPILGGVLRGRAAHARVAAFWDRFV
                     VEGRGVRCVSDRPWVTAAETCELVMALDAVGLDEQARSLLTWVQFLRADGGGYWTGAN
                     FDAERFDGPGELFPVEQPSWNSAAVVLAANALGGAGPTAGLFRGEGLPGGLTADELIE
                     AGAEIERELATRRRERD"
BASE COUNT          616 a         1462 c         1442 g          620 t
ORIGIN      
        1 cggcaccggg gcgatcgacg cccagccgct cgaggaggtc gaccggatcc tggccgtgaa
       61 cctgcgcggg gtgatcctcg gcctccgcgc cgcggtcccc acgttgcggg ccggcggcgg
      121 agggtcgatc gtcgtcaccg cgtcggtgtc cgggctgttc ggcgaccccg ggctcttcgc
      181 ctacaacgcg tcgaaagccg gtgccgtcaa cgtggtgcgc gccgcggcgc tcgacctcgg
      241 ccgtgacggc atccgcgtca acgcggtgtg cccgggcccg atccgtggga ccggcatgac
      301 cggaccactc gaacgccgcg ccggtgacct gtacgccgag atgcgaagcc atctgccgct
      361 cggccggttc ggcgaaccgg ccgaagtcgc cgcggtcgca gcattcctgc tgtcggacga
      421 cgcgtcgtac gtcaccggtg tcgccttgcc ggtggatggc ggggtcaccg cgggcacggg
      481 gcagttccgg gcggtgtacc ccgccgagtc cggatagacg ctcgggctcg tcggccgccc
      541 agcgcggcgg ccgacgaggc gcctgtcacc ccgtcagctg ggtcatcaac gcggtggtat
      601 cgaagtagtc gcgccagcgg ctcaccttgc cgtcacgaac ctcgaagtag ccggcgacgc
      661 gggcctcggc ctgcttgccg cccggcaaga agaagcggtc gacccgctcg ttcatgacca
      721 catcgccctg ctcgacctgg gcgaggacct cccaggcgac ccgctcggtg ccggcataga
      781 acgggccgag gagggcccgg acctcatccg cgccgttgca gacgcccatc ccgacgttct
      841 cgtaagcgat gtcatcggtc atgagggcga cgatggcgtc gaggtctcgg gcctcgttcg
      901 ccttgatcaa cgcggcgacg acttctccgg aacccatagc tgctcccgtc tgtggcggcg
      961 gcgggacggt agcagccagg gccgagcggt accgtgggat ggatgagcga cgcgcgcgag
     1021 cggtggcagt cggcgttcga cgcgacaccg agccgcgacg ccgatttcga gacgatgtcg
     1081 gggatgccgg ttgagccggt ctacgggccc gacgagtcgc tccctggcgc gtggccctac
     1141 acgcgaggcc cgtacgcctc gatgtaccgc tcgaagctgt ggacgatgcg gatgttcgcg
     1201 gggttcggga ccgcgatcga caccaaccga cggttccgag agctgctcgc ggctggcggc
     1261 agcgggctct ccaccgcctt cgatctgccg acgctcatgg gtcgcgacag cgacgacgag
     1321 ctggcgctcg gcgaggtcgg caagggcggc gtgtcggtcg attcgctcgc cgatgtcgtg
     1381 gacctctacg ccgggatcga cctcggtcac gtcacgacgt cgatgacgat caacgccccc
     1441 gcgccgatcc tgctcgccat gtatgtcgcc gcggcggaag cgaccggtgt cgcgcgtcgc
     1501 gatctcggcg ggacgatcca gaacgacatc ctcaaggagt accaggccca aaaggagttc
     1561 gtgttcccgc cgcgaccgtc gatgcgcctc gtcaacgaca ccatccgctt ctgcgaggcc
     1621 gagatgccgc gctggcatcc ggtgtcgatc tccgggtacc acatccggga ggcgggctcg
     1681 accgcggtgc aagagctcgc cttcaccctg gcgaacggtt tcgcgtacgt ggagcaggcc
     1741 gccagctcgg ggttggcggt cgacgagttc gctccccggc tctccttctt cttcaacgcc
     1801 cacatcgact tcttcgagga gatcgccaag tatcgagcag cgcggcgaat ctgggcgcgc
     1861 tggatgcgag accggtacgg cgcccatgag gaacgctcca tgcagctgcg cttccacgcc
     1921 cagacggccg gggtgtcgct caccgcgcag caacccgagg tgaacctggt tcgcaccgcg
     1981 gtggaggcgc tcgccgcggt cctcggcggc acccagagcc tgcacaccaa ctccatggac
     2041 gagacgatgg cgctcccaac cgacaagtcg gcgcgtctgg cgttgcgcac ccagcaggtc
     2101 ctggccttcg agaaccgggt cgcgaacgtc gccgacccgc tgggcggctc gtggtacgtc
     2161 gaggccctca ccgacgagat ggaacgccgc gccgaggcca tcttcgagca gctcgagggg
     2221 ttcggagacg gatcgatgct ggaaggggtc ctgcgggcgg tcgagtccgg ctggttccaa
     2281 cgcaacatcg ccgactccgc gtacgagctg gagcgcaaga cgaacagcgg ccacaacgtg
     2341 gtcgtcggcg tcaccgactt ctttgaaggc aacgaggaag ccccgccgga gctgctgcgc
     2401 atcggccccg agttcgagga acgccagctg aagcggctcg ccgcgctccg agcggatcgc
     2461 gacgacgggc gggtcgacgc ggtgctggcc cgggtccggg ccgacgccgc cgagccgacg
     2521 acgaacgtcc ttcccgccct catcgaggcc gtgcagggcg acgcgacact gggcgagatc
     2581 gtcaacgcct tcgccgacgt gttcggtcgg tacaccgaag cgccgatgat ctgaccgccc
     2641 ccgtggatcc cgatctgttc cagcacgccc gcgccgcgtt gggcttcatg tctgacgacg
     2701 agggcctcgc gctccacgac gcggggctcg ccgcggcggc catgggtccg ctcctcgagg
     2761 tcggcaccta ttgcgggaag tcggcgatct atctgggcgc cgcggcacgc gaacgcgaca
     2821 ccgtcctgtt caccgtcgac catcaccggg gctcagagga gaaccaggct ggttgggaat
     2881 ggcacgacga acgcctcgtg gatcccgagt cgggtcgtct cgacacgctt ccgtggtttc
     2941 ggcgcaccat cgccgccgcc gcgctcgaag ccacggtcgt ggccgtcgtg ggcgactcgc
     3001 cgaccgtggc gcggcactgg cggacaccgc tcgggctggt gttcatcgac ggcggccacg
     3061 ccttcaacgt ggccgccgcc gactacctcg gctgggcgcc ccaggtcgcg cccggcggtc
     3121 tcctggtctt ccacgacgtg ttcgaggacc cggccgacgg cggccaagcc ccgttcgagg
     3181 tgtggcgtcg cgccgtctcg gacgggttca cgccggtggc gacgaccggg tcgttgcgcg
     3241 tcttgcgacg ctgaccgtca gtcccgctcg cggcgacggg tggcgagctc gcgttcgatc
     3301 tcggcgccgg cctcgatgag ctcgtcagcg gtcaggccgc cgggtagccc ttcaccgcgg
     3361 aacagacccg cggtcggtcc ggcaccgccg agcgcgttcg cggccagcac caccgcggcg
     3421 gagttccagc tcggctgctc caccgggaac agctcgcccg gcccgtcgaa gcgctcagca
     3481 tcgaagttgg cgccggtcca gtagccgcca ccatcggccc ggaggaactg gacccaggtg
     3541 agcaacgatc gggcttgctc gtcgagcccc accgcgtcga gcgccattac cagctcgcag
     3601 gtctcggccg cggtcaccca gggccggtct gagacgcacc gcacgccgcg gccctcgacg
     3661 acgaaccggt cccagaacgc cgcgacccga gcgtgcgcgg cccggccccg taagacacca
     3721 ccgaggatcg ggtagtacca gtccatcgcc cagcgggcct tctcgaggaa gtggtcgggc
     3781 cgatgagcga ctgcgatggc gagcgcaccg agtgacagct cccagtcggg acgctcatga
     3841 ccgagccgct cggcgatggc gatcgcgcag cggaggctgg cgtggatgct cgacgatccc
     3901 gtcaggagcg ccccgttggc cgggtcgtca ccgcgccacg cgatctcgcc cgtctcggcc
     3961 tggtgggaga gcgcgaagtc gattcctcgt tccacgaccg accagaactc ctcgaggaac
     4021 ccggtgtccc cggtgctcag gtagtggtgc cacaccccgt tggcggagta gcaggtcacg
     4081 ttggtgtcga gggcccagtc cttgacgtcg cgcccgacgt agtaggcgtg ccagcagccg
//
LOCUS       DATCCH010000280         3601 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_114092, whole genome shotgun sequence.
ACCESSION   DATCCH010000280 DATCCH010000000
VERSION     DATCCH010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114092"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VIJ44_05380"
     CDS             complement(<1..467)
                     /locus_tag="VIJ44_05380"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HEY5165366.1"
                     /translation="MSTQADIGHLLRRATFGPFPGQVDSLVPAGTGAALNSVLTSAPS
                     ALPAPPGYVYLSIEPVEWWLRRVRDPGAGLVEKMVLFWHGLLVSAQDKVNDWEWMWEQ
                     NLLFRQYALGNFRSLLQAITIDRAMLFYLDGNRSNAASPNENFGRELMELFTLG"
     gene            complement(655..728)
                     /locus_tag="VIJ44_05385"
     tRNA            complement(655..728)
                     /locus_tag="VIJ44_05385"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(692..694),aa:Ala,seq:ggc)
     gene            856..>3601
                     /locus_tag="VIJ44_05390"
     CDS             856..>3601
                     /locus_tag="VIJ44_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018726766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEY5165367.1"
                     /translation="MNDDATPAASATPRYRYDARRANELESRWQDYWEEHRTFWTPNP
                     GGPLTDGFERVADRPKLYVLDMFPYPSGAGLHVGHPLGYIGTDVYARYQRMTGHNVLH
                     AMGYDAFGLPAEQYALQTGQHPRVTTEQNITNMRRQLRALGLGHDPRRGVATTDVQYY
                     RWTQWIFLRIFNSWYDDDADRARPIDELIAELEAGAREPVSDANPDARPWSELDDRTR
                     RRVVDAYRLAYREEAPVNWCPALGTVLANEEVTSEGRSERGNHPVFKRPLEQWMLRIT
                     KYADRLLADLDLIDWPEPIKVMQRNWIGRSTGASILFPVEAHEGLAVEVFTTRPDTMF
                     GATYLVLAPEHPLVDRIVPTDWPGALLSDETTAVPAAWRGVFGADVTPAEAIIGYREF
                     VSGRTELERQAEGREKTGVFTGAFAENPATGGPIPVFVADYVLMGYGTGAIMAVPGED
                     QRDWDFATAFDLPIVRTVQPPSEFSGEAYAGDGPAMNSGFLDGLHIEDAKRRIIEWLD
                     TNGNGTGTVTYKLRDWLFSRQRYWGEPFPIVYGDDGLPIALPDSMLPVELPDLEEFEP
                     VVTDDAADTPPEPPLARAQHWVDVTIDLGDGPRRYRRETNTMPQWAGSCWYYLRYLDP
                     TNENRLVDPDVERYWMAGDGGGGVDLYVGGVEHAVLHLLYARFWHKILSDLGYVSTPE
                     PFHRLFNQGYIQAPAFQDARGLYVEASEVVEHGGQLTFHAEPVTREYGKMGKSLKNVV
                     TPDDVYRDYGADTLRLYEMFMGPLDASRPWSTNDIVGVHRFLQRLWRNVIDEETDVVR
                     VGTTPADPETRRLLHRTIAAVGADMAALKFNTAIARLFELNNQLTRLVAETGESPREV
                     VEPLVLMLGPLTPHIAEELWARLGHQGGLAFEPFPEPDPDQLVVDTVEVAVQVNGKTR
                     G"
BASE COUNT          633 a         1282 c         1150 g          536 t
ORIGIN      
        1 ccgagcgtga acagctccat gagctcacgt ccgaagttct cattcgggct ggcggcattg
       61 gagcggttgc catcgagata gaagagcatt gctcgatcga tggtgatggc ctgcaggagg
      121 gagcggaagt ttccgagcgc gtactggcgg aacagcaggt tctgctccca catccattcc
      181 caatcgttga ccttgtcctg ggccgacacc agcaggccgt gccagaacaa caccatcttc
      241 tcgaccagcc ccgcgccggg gtcgcggacc ctgcgcagcc accactcgac cggctcgatc
      301 gacaggtaga cgtagcctgg gggagcgggc agcgcggacg gcgccgaggt gagcaccgag
      361 ttgagtgccg cgccggtccc ggccgggacc agcgagtcga cctggcccgg gaacggcccg
      421 aaggtcgcgc ggcgcaggag gtgcccgatg tcagcctggg tggacatcgc gccgttatcg
      481 gcacaaacgc aggattgcta tagaccgccg cacgggtcgg atgcgccggg acgctcgggt
      541 gccgcacagc aaccacgtcg tggtggaccg aaggcgaggt gttcgaaccc cgcccttcga
      601 gaagggacga ttcgcctgtt gacctgcttg aacgttgtgc attcgacaac cgcgtggacc
      661 tgaggggact tgaacccctg acttcctcca tgccatggag gcgctctacc ggactgagct
      721 acaggcccag gggagacgca acggtaccag tggccctcgg gacagcccgt ggtgctctac
      781 gaacgaccgt tcgacctgtc ggcttcggat agggggccag ggtcggtcaa ggtcgtcgct
      841 acgctcgccg tcgccatgaa cgacgacgcg acgccggctg cgagcgcgac cccgcggtac
      901 cggtacgacg cgcgccgcgc caatgagctc gagtcgagat ggcaggacta ctgggaggag
      961 catcggacct tctggacccc gaatcctggc ggcccgttga ccgacggctt cgaacgggtg
     1021 gcggaccggc ccaagctcta cgtgctggac atgttcccgt acccgagtgg cgcgggcctc
     1081 cacgtcggtc atccactcgg atacatcggc accgacgtct acgcccgcta tcagcgcatg
     1141 accggccaca acgtgctcca cgcgatgggg tatgacgcgt tcgggctccc agccgagcag
     1201 tacgcgctcc aaaccggcca gcaccctcgc gtcaccaccg agcagaacat caccaacatg
     1261 cgccgccaac tgcgcgcgct cgggcttggc cacgacccgc gccggggagt ggccacgacc
     1321 gatgtccagt actaccggtg gactcaatgg atcttcctcc gcatcttcaa ctcctggtac
     1381 gacgacgatg ccgaccgggc ccgtccgatc gacgagctca tcgccgagct cgaagccggc
     1441 gcccgtgagc cggtcagcga cgcgaatccc gacgctcggc catggtccga gcttgacgac
     1501 cgaactcgac gccgagtcgt tgacgcctac cggctcgcct atagagagga agccccggtc
     1561 aactggtgcc cagcgctcgg cacggtcctc gccaacgagg aggtcacctc cgagggtcgc
     1621 agcgagcggg gcaaccaccc cgtcttcaag cgcccactcg agcagtggat gctgcgcatc
     1681 accaagtacg ccgatcggct cctcgccgat ctcgacctga tcgactggcc cgagccgatc
     1741 aaggtgatgc agcgcaactg gatcgggcgg agcaccggtg cctccatcct cttccccgtc
     1801 gaagctcacg agggtctcgc cgtcgaggtg ttcaccaccc gccccgacac gatgttcgga
     1861 gcgacgtatc tcgtcctcgc acccgaacat ccgctcgtgg accgcatcgt gccaaccgac
     1921 tggcccggcg cgctgctgag cgacgagacc actgccgtcc cggcggcctg gcgcggggtc
     1981 ttcggcgccg acgtcacacc ggccgaggcc atcatcggtt accgcgagtt cgtctcggga
     2041 cgaaccgagc tcgaacgcca agccgagggg cgcgagaaga cgggcgtgtt caccggggcc
     2101 ttcgccgaga acccggcgac cggtggcccg atcccagtgt tcgtcgcgga ctacgtcctg
     2161 atgggctacg ggacgggcgc gatcatggcc gtccccggcg aggaccagcg cgactgggat
     2221 ttcgccaccg cgttcgacct cccgatcgtc cggacggtcc agccgccctc cgaattcagc
     2281 ggagaggcct acgccggtga cggaccggcc atgaacagcg ggttcctcga cggcctccac
     2341 atcgaggacg ccaaacgccg catcatcgag tggctcgaca cgaacggcaa cgggaccggc
     2401 accgtcacct acaagctgcg cgactggctg ttcagccgcc agcggtactg gggggagcca
     2461 ttcccgatcg tctacggcga cgacggtctc ccgatcgcgt tacccgactc gatgctgccc
     2521 gtcgagctcc ccgacctcga ggagttcgaa ccggtcgtga ccgacgacgc ggccgacacg
     2581 ccaccggagc cgccgctggc ccgcgcccag cattgggtcg acgtcaccat cgacctcggc
     2641 gacggcccgc gccgctaccg ccgcgagacg aacaccatgc cgcagtgggc cggctcctgc
     2701 tggtactacc tgcgctacct ggacccgacg aacgagaatc gtctcgtcga cccggacgtc
     2761 gagcgttact ggatggccgg cgacggcggc ggcggcgtcg acctgtacgt cggtggggtc
     2821 gagcacgccg tgctgcacct gctgtacgcc cgcttctggc acaagatcct ctcggacctc
     2881 ggctacgtct cgacgcccga accgttccat cggctcttca accagggcta tatccaagct
     2941 ccggcgttcc aagacgcccg cggcctctac gtcgaggcgt ccgaagtcgt cgaacacggc
     3001 gggcagctca cgttccacgc cgagcccgtc acccgcgagt acgggaagat gggcaagagc
     3061 ctgaaaaacg tcgtcacgcc cgacgacgtg taccgcgact acggggcgga cacgttgcgg
     3121 ctctacgaga tgttcatggg ccccctcgac gcgagccgac cgtggagcac caacgacatc
     3181 gtcggggtcc accggttcct ccaacgcctg tggcgcaacg tcatcgacga ggagaccgac
     3241 gtcgtgcggg tcgggacgac acccgccgac cccgagacgc gccgcctcct gcatcgcacc
     3301 atcgccgcgg tgggcgctga catggccgcg ctgaagttca acaccgccat cgctcggctc
     3361 ttcgagctca acaaccagct cacccgcctc gtcgccgaga ccggcgaatc gccgcgtgag
     3421 gtggtcgaac ccctggtgct gatgctcggc ccgctcaccc cccacatcgc cgaggagctc
     3481 tgggcccgcc tcggccacca gggggggctc gcgttcgagc cgttccccga accggaccct
     3541 gaccagctcg tggtcgacac cgtcgaggtc gcggtgcagg tgaacggcaa gacccgaggt
     3601 c
//
LOCUS       DATCCH010000281         4539 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_570488, whole genome shotgun sequence.
ACCESSION   DATCCH010000281 DATCCH010000000
VERSION     DATCCH010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4539
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570488"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(245..556)
                     /locus_tag="VIJ44_05395"
                     /pseudo
     CDS             complement(245..556)
                     /locus_tag="VIJ44_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020557181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
     gene            675..1061
                     /locus_tag="VIJ44_05400"
     CDS             675..1061
                     /locus_tag="VIJ44_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165368.1"
                     /translation="MGGEHQPRTAGSPAIQFTQAARRLGAAARAAGLSVPAFRSPPRV
                     AGARRTLRRYPDGAVVSVTLRGRPFDDVVVDMVEGLVRVNRLDGDAARRFRGELLHAV
                     TDEPTAGRGDLPSPRARMAERQTQAA"
     gene            1026..1108
                     /locus_tag="VIJ44_05405"
     tRNA            1026..1108
                     /locus_tag="VIJ44_05405"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1059..1061,aa:Leu,seq:taa)
     gene            complement(1306..1992)
                     /locus_tag="VIJ44_05410"
     CDS             complement(1306..1992)
                     /locus_tag="VIJ44_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006330448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY5165369.1"
                     /translation="MEVDRTPAEPPKSGRPDRRARRRQETIEEILDIAEDVMTEEGVT
                     GLSLAEVARRLGVQPPSLYKYFPSLRAVYDALFLRGQREHLAVMRQAMADAEPGLAAL
                     TTGLEASGRWLLANRAIAQLLFWRPVPSFEPSPEAFAPSVEMAALQRAALADAVAAGQ
                     LGPEADSDEAVYLVSTLISGVLTQALANEPHLEWGQGRFTPTFPKLMRLLVAAYPLPR
                     SGAKRRSHSR"
     gene            2081..2572
                     /locus_tag="VIJ44_05415"
     CDS             2081..2572
                     /locus_tag="VIJ44_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019874877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY5165370.1"
                     /translation="MPAKNGRWWLTEDSEEITVHAAAEAIYGLVAELTRMGEWSPECQ
                     RVEWEDGATGPAAGARFVGHNRGGPLQLMRWSRRGRVLTAEPGREFAFVTEEGGREST
                     LWRYRFEPVEGGTRVTESYEVKWIPAWARIVDVPTNRHRELQAAMRHTLEQLRTAAEA
                     TTT"
     gene            complement(2812..3291)
                     /locus_tag="VIJ44_05420"
     CDS             complement(2812..3291)
                     /locus_tag="VIJ44_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5165371.1"
                     /translation="MGLVVAADDPRVDDVRALLERNLAFARGVTPPAGVHALDVEGLL
                     DPTVTFFSARLDGELLGVGALKQLDESHAELKSMHTVEAVRGQGVGRALVDHLLSVAA
                     DRKYQRVSLETGTMDAFAPARSLYTKVGFRPCTRFREYAQSPTSACMTIAIDPANRP"
     gene            3694..4242
                     /locus_tag="VIJ44_05425"
                     /pseudo
     CDS             3694..4242
                     /locus_tag="VIJ44_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007773679.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
     gene            4312..>4539
                     /locus_tag="VIJ44_05430"
     CDS             4312..>4539
                     /locus_tag="VIJ44_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl dehydratase"
                     /protein_id="HEY5165372.1"
                     /translation="MPKFDLDVTATVVVAGALASRDFMPVHHDRDYARAQGAPDMFMN
                     ILTTNGYVSRYVTDWAGPEAMLRSMAIRLGGP"
BASE COUNT          753 a         1560 c         1543 g          683 t
ORIGIN      
        1 cgctgcgacc ccagccgtcg acgtccccgc gtcgcagctc ttcgtcgtct gggacggggg
       61 tcgggtgccg caccgggacc agctcaccgc cgctcacggt cgggtcggtc caagcgacgt
      121 tcggcgccaa cgggcggtcg atgggctccc tcggcaccgc gccagtgaac ccgcatgacc
      181 gcatgcgagg caacctggga gggcgtcgac gatcaccgac gacaaccccc aacccaacca
      241 ccgtcaacgc gctcccgatc ccaagggcca gccacacgcc atccccccga gctccgcggc
      301 ccggcgacgg tccccggaac cgagcgagcg ggccaccccc gcggtggtcg agtacgccaa
      361 gaagttgaac accgagaacg cggacgacag aacgatcccg gccacggcca acccggccag
      421 cggcccgggt tcccaggcga cccacgatgg ccgtgtccgc caggacatag agcggctcgg
      481 ccacgagcgc cccgaacgcc ggcaccgcca gccgcacgat ctcccggtca tggggactac
      541 ggcgggtgat cgtcacaacc cgcccgccga ccgtcgagcg gccccgcccg gctcgacacc
      601 gcccccactg cagccgctca tgggcgacag ggtaaaaggg cgccaccgtc acaccccagc
      661 gcgatcctga gtctgtgggc ggagaacacc aacctcgtac ggccggctca ccggcgatcc
      721 agttcaccca ggccgcgcgc cgactcggcg ccgcagctcg ggccgccggc ttgagcgtcc
      781 ctgccttccg gtccccacca cgggtcgccg gggctcgccg caccttgcgc cgctaccccg
      841 acggagccgt cgtctccgtg accctgcggg gtcgaccctt cgatgatgtc gtcgtcgaca
      901 tggtcgaagg tctcgtccgc gtcaaccggc tcgacggtga cgccgcccgc cgcttccggg
      961 gagagctgct ccacgccgtc accgacgagc cgaccgccgg gcgtggcgac ctaccctcgc
     1021 ctcgagcccg gatggcggaa cggcagacgc aggcggctta aacccgccgg cccctcgggg
     1081 cgtggaggtt cgactcctcc tccgggcacc gcctcttgta gaccacgtct cggtacgaac
     1141 gtgaccagca gttgccgatc cctcagagct tggtcctcgt gagcgactcc tctcgagctg
     1201 ctccggagcg ccataccctc gacgccgcga cggtcggatg gcgtacgtgg tcggttctgt
     1261 tccggcacac gcgacgtctc gtttcggtct cgtctccccg cgatgtcagc ggctgtgact
     1321 gcgccgcttc gcccccgacc tcgggagggg gtatgcggcg acgaggagcc gcatgagctt
     1381 cgggaaggta ggggtgaagc ggccctggcc ccattcgagg tggggctcgt tggccagggc
     1441 ctgggtcagt actccgctga tcaacgtcga gacgagatag acggcctcgt cggagtcggc
     1501 ctcggggccg agctggccgg cggcgacggc atcggcgagc gcggcgcgtt ggagggcggc
     1561 catctcgacg ctgggcgcga acgcttcggg ggagggctcg aagctcggga cggggcgcca
     1621 gaacagcagc tgggcgatcg cccggttcgc tagcagccag cgtccgctcg cctcgaggcc
     1681 tgtcgtcagg gcagccagtc ccggctcggc gtcggccatg gcttggcgca tgactgcgag
     1741 gtgttcccgt tggcctcgca ggaagagcgc gtcatagacg gccctgagcg aagggaagta
     1801 cttgtagagc gaagggggct ggacaccgag gcggcgggct acctcggcca ggctgaggcc
     1861 cgtcacccct tcttcggtca tcacatcctc ggcgatgtcg aggatttcct cgatggtctc
     1921 ctggcggcgt cgggcgcgcc ggtccggccg tccggacttc ggtggctcgg ctggggtgcg
     1981 gtcgacctcc atgaggggca gcctagcaaa agcttgactt gacagcaata gctatgaccc
     2041 gtagcctacg gacgaaggaa actgagagag aggaaatggg atgcctgcga agaacgggcg
     2101 ctggtggttg accgaggaca gcgaggagat caccgtccac gcagctgccg aagcgatcta
     2161 tggcctggtg gccgagttga cgcgcatggg cgagtggagc ccggagtgcc aacgcgtcga
     2221 gtgggaggat ggcgcgaccg gtccggccgc gggcgcgagg ttcgtcggcc acaaccgggg
     2281 cggaccgctt caactcatgc gctggtcgcg tcgcggccgc gtcctgaccg ccgaaccggg
     2341 tcgcgagttc gcgttcgtca ccgaggaggg cgggcgcgag tccacgctct ggcggtaccg
     2401 gttcgagccg gtcgagggcg gcacccgggt caccgagtcc tacgaggtga agtggatccc
     2461 ggcctgggcg cggatcgtcg acgttcccac caaccgccac cgcgagctgc aagcagccat
     2521 gcgccacacg ctcgagcaac tcaggacggc cgccgaagca acgacaactt gacgggacac
     2581 gtcgtgatcg cttcgtacaa ggcaccgccc gacatcgatc gctcgaacag atactgggcc
     2641 ccaatgaccg gcggagagtc tccgccggta cagcgccggc acccgctcag accgcgcacc
     2701 aaccgcaaca ggtgggaaca gcctccatct ttcatcccgc gggatccctt acgacagcgt
     2761 gtggagcgac ctgccaacag ccgggaaggc cctcggctca ccgctcgtcg ttcatggccg
     2821 gttcgcgggg tcgatcgcga tcgtcatgca cgcgctggtc ggactctgtg cgtactcccg
     2881 gaatcgcgta cacggcctga aaccgacctt cgtatagagc gagcgagctg gtgcgaaggc
     2941 gtccatggtt ccggtctcga gactcacccg ctgatacttc cgatcggccg cgaccgacag
     3001 gagatggtcg actagtgctc gtccgacccc ttggcctctg acggcctcga ccgtgtgcat
     3061 tgacttcaac tcggcgtgag actcatccag ttgcttgagt gccccgactc caaggagctc
     3121 gccgtcgagg cgggcgctga agaatgtgac ggtcggatcg agaaggccct cgacgtccag
     3181 ggcgtggaca cctgccggag gtgtgacccc tcgagcgaac gctaggttcc gctccaacag
     3241 ggctcgaacg tcgtcaacac gtggatcgtc ggccgcaacg acaagaccca tgaacgtcgt
     3301 ctaccaacga cggagggatc gatgcaactg gaacaacgca acacctcgcc ggaaggttca
     3361 ggcgacccgc gcaccgggca tccccgtgga tgggtcgacc gcgcggtcca gaccgccttc
     3421 gagccgacgt ggtcaccagc gccagatgtc agcctcacgt gaacatggtc cgttcggagc
     3481 gcgacttctg ccccgacgtg cccacgactc atgtcgctct tgcagtaccg agggcccgaa
     3541 gtcgactcag tgctactcgg gcagtggtac cgctgggtgt ctcacgcgtg tgccgacaac
     3601 ttggggcgcg ggccagcttc ggcggcctct cgaccgcacc aggaacgccg tgacgggagg
     3661 gagcaccggg tgcattctcg gtacgagagt gtgatgacgg gcgaggggtc ggatccactc
     3721 cgggagcagg tcgaggcgtg gatcggacgg ccgatgagcg cgtcgggacc cgcggtcgca
     3781 cccgacgacg tcaacctgcc catgatccgc cactgggtgg atgccctcga cgaccgtaac
     3841 cccgtctacc tcgacgcggc cttcgccgcg aggacccggt tcggggggat cgtcgcccct
     3901 ccggcgatgc tgcagacgtg gacgatggct cgtcctcgga tcgagggaat cgcagctcgg
     3961 ggtggtgcgg cagcagagat cgacgtggac agcccgatcg gcatcctcac cgcgaacggc
     4021 ttcacgggca ccttggcgac gaactccgag ctcgagttcg cgcggtactt gcgcttgggg
     4081 gaccgattgc agtcgtccac cgcgttggat tccgtctcgg agcggaagtc gaccgcgctc
     4141 ggactgggat acttcgtgac ctgggtgacg acgtacaccg acgatcaagg cgacgtcgtg
     4201 ggccggcagc gtttccggat ctgcaagttc gcgccgggga gcactgcgcc ggtcgcagcg
     4261 gccggtcgtg agcgacgcca gccgtccggc gaggcgagcg tcggggagga gctgccgaag
     4321 ttcgaccttg acgtcacggc caccgtcgtg gtcgccggtg ctctcgcgtc tcgtgacttc
     4381 atgcccgtgc accacgatcg cgactacgcg agggcgcagg gagcaccgga catgttcatg
     4441 aacatcctca ccaccaacgg ctacgtctcg cgctacgtca ccgattgggc cggtccggag
     4501 gcaatgctgc gcagcatggc gatccgcctc ggtgggccg
//
LOCUS       DATCCH010000282         2204 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_389412, whole genome shotgun sequence.
ACCESSION   DATCCH010000282 DATCCH010000000
VERSION     DATCCH010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389412"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /gene="menD"
                     /locus_tag="VIJ44_05435"
     CDS             complement(<1..876)
                     /gene="menD"
                     /locus_tag="VIJ44_05435"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HEY5165373.1"
                     /translation="MRRDANTAFARTLVDEWVRAGVRHACVAPGSRNAPLALALVEDG
                     RLRVHVHLDERSAAFFALGCARASGQPAVVCCTSGTAAAHLHAAVLEAAHGRVPLLVC
                     TADRPPELRDTGAGQTINQVGLYGDTPRWSIDVEAPTDRAGIGPWWRSLAARAASSAL
                     GPPAGPVHLNLAFREPLAPTGAELVDAPGRVDGRPWTVTTRPVRTADAATVERLAQTI
                     RDVPKGLLVAGWGSGLNAPLVTRFARASGWPVLADALSGLRACDGAVSTYDAMLRVDE
                     FVATHRPDLVVRVGAP"
     gene            891..1601
                     /locus_tag="VIJ44_05440"
     CDS             891..1601
                     /locus_tag="VIJ44_05440"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY5165374.1"
                     /translation="MRLRLIPGFTNTARSWEPVERGLPVDWDVQAVDIPDGLDFVETA
                     DALAHRGGSGTWVGYSMGARLALRLALDNPSFVAALVLVSGSPGIASAGTRETRVAAD
                     ERQAQELERDGVDAFLERWLDQRLFETLPREAAMLDDRRRGNTVRRLAHQLRELGPGA
                     QEPLWDRLAELAMPVLLIAGGYDRAYSETAQRMATAIGSNAEVAILPRTGHAVHLEQP
                     KELAQLLTSWAGAVGPVE"
     gene            complement(1556..>2204)
                     /locus_tag="VIJ44_05445"
     CDS             complement(1556..>2204)
                     /locus_tag="VIJ44_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012950538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEY5165375.1"
                     /translation="DLATLAAYPAVKVKVGFDGDVDLVAAVRDAIGPRVALRVDANGA
                     WDVDTASSQLARLARYDLELAEQPVATLDDLARLRRRTNVPVAADECVRTPRDARRLR
                     ELHAADALVLKVQPCGGVRAALALADLAGVPAIPTSMHETSVGVAAGVALAAALPDLL
                     YACGLATVTSLDADVTNDPLVPTGGVLRRRVVTPDPSLLARYSTGPTAPAQEVRS"
BASE COUNT          313 a          742 c          873 g          276 t
ORIGIN      
        1 cggcgcgccg acccgcacga ccagatccgg gcggtgggtg gccacgaact cgtcgacgcg
       61 gagcatggcg tcgtaggtcg agacggcgcc gtcgcacgcg cgcaggcccg agagggcatc
      121 ggcgaggact ggccaaccgg aggcgcgtgc gaaccgcgtc acgagcggcg cgttgaggcc
      181 ggatccccag ccggcgacga gcagcccctt gggaacgtca cggatggtct gggcgaggcg
      241 ttccaccgtc gcggcatcgg cggtgcgcac cggtcgggtc gtcacggtcc agggtcgccc
      301 gtcgacccgg ccgggggcgt cgaccagctc ggccccggtc ggggcgagcg gctcgcggaa
      361 ggccaggttg aggtggacgg gcccggccgg agggccgagc gcactcgagg cggctcgcgc
      421 cgcgagcgag cgccaccagg gtccgatccc ggcccggtcg gtcggtgctt cgacgtcgat
      481 ggaccaccgt ggcgtgtcgc catacagacc gacctggttg atggtttgtc cggcgccggt
      541 gtcccgcagc tcgggtgggc ggtccgcggt gcagaccagc agcggaacgc ggccgtgcgc
      601 cgcttcgagg acggccgcat gaaggtgcgc ggcggcggtc ccggacgtgc agcacacgac
      661 tgcgggttgg ccggaggccc gggcgcaacc cagggcgaaa aacgccgcgg atcgttcgtc
      721 gaggtgcacg tgcacgcgga gacggccgtc ctcgacgagg gccagcgcga ggggcgcgtt
      781 gcgggacccg ggtgcgacgc aggcgtggcg gacgccggcc cgcacccatt cgtccacgag
      841 ggtgcgggcg aatgcggtat tggcatcacg cctcatggaa ggatccaacc gtgcggctgc
      901 gactcatccc agggttcacc aacaccgccc ggtcctggga accagtggag cggggattgc
      961 cggtggactg ggacgtccag gccgtcgata tccccgacgg gctcgatttc gtcgagacgg
     1021 cggacgcgct tgcccaccgg ggcggctctg ggacttgggt cggctactcg atgggggccc
     1081 gtctggccct gcgcctcgcg ctcgacaacc cgagcttcgt ggcggcgctg gtgttggtga
     1141 gcggctcgcc cgggatcgcg tcggcgggca cccgcgagac ccgggtggcg gccgacgaac
     1201 gccaggccca agagctcgaa cgggacgggg tcgacgcgtt cctcgagcgg tggctcgacc
     1261 agcgcctctt cgagacgctg ccccgcgagg cggcgatgct cgacgatcgc cgccgcggca
     1321 acacggtccg ccgattggcg caccagctgc gggagttggg gcccggtgcc caagagccgc
     1381 tctgggatcg cttggctgag ctggcgatgc cggtgctgct catcgccggc ggctacgacc
     1441 gggcctactc cgagaccgcc caacggatgg ccacggcgat cgggtcgaac gcggaggtgg
     1501 cgatccttcc ccggaccggc catgcggtgc acttggagca gccgaaggag ctggctcaac
     1561 tcctgacctc ttgggcgggc gcggtcgggc cggtggagta gcgggccagc agggacgggt
     1621 cgggcgtcac cactcgacgc cggaggacgc cgcccgtggg gaccaggggg tcgttcgtga
     1681 cgtcggcgtc gagtgaggtg acggtcgcca ggccacacgc atagagcagg tccggcagcg
     1741 cggccgcgag ggcaacgccc gccgcgacgc ccaccgaggt ctcgtgcatc gaggtgggga
     1801 tggccggcac cccggcgaga tcggcgaggg cgagcgcggc gcgcacgcca ccgcagggct
     1861 ggaccttcaa gaccagcgcg tcggcggcgt ggagctcccg gaggcgacga gcgtcgcggg
     1921 gcgtgcggac gcattcgtcg gcggcgaccg gcacgttcgt acggcgccgg agtcgagcca
     1981 ggtcgtcgag ggttgcgacg ggctgctcgg ccagctcgag gtcgtaacgg gcaagccgcg
     2041 ccagctggga cgaagcggtg tccacgtccc aggcgccgtt ggcgtcgacg cgcaacgcca
     2101 ccctcgggcc gatcgcgtcg cggaccgcgg cgacgagatc gacgtccccg tcgaagccga
     2161 ccttgacctt caccgcggga taggccgcga gcgtcgcgag atcg
//
LOCUS       DATCCH010000283         3774 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_197586, whole genome shotgun sequence.
ACCESSION   DATCCH010000283 DATCCH010000000
VERSION     DATCCH010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3774
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197586"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..120
                     /locus_tag="VIJ44_05450"
     CDS             <1..120
                     /locus_tag="VIJ44_05450"
                     /inference="COORDINATES: protein motif:HMM:NF013768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165376.1"
                     /translation="ELDRRPAGIVGILGPTRMDYRHALAAVTAVSEQLGRHLS"
     gene            117..1220
                     /locus_tag="VIJ44_05455"
     CDS             117..1220
                     /locus_tag="VIJ44_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018216605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEY5165377.1"
                     /translation="MTRDHYEVLGVDRHASDEDIKRAYRDLARRYHPDSQPDAPDAEE
                     RFKEISVAYEALRDPERRRRYDLFGDTGAARSGGGETFGFGDLFDAFFAGDPFGARRG
                     QAGPTRGADAEVVVQLTLTDTMFGKTETIDASLPVSCPRCEGSGCEPGTHPTRCDVCG
                     GAGEVRQVRRSILGQIVTAAPCGVCEATGRRILSQCRDCRGDGRIQAHRSIDVEVPAG
                     VGDGQRLRLAGRGPAAPRGGIPGDLYVTVRVAAHPDFERHGDDLLHVRQVSVAQAALG
                     TRLTVTGLDAEHEVLVPPGTQPGQLFRVKHVGIPSLRGRGRGDLLVRVDVEVPTRLSE
                     EEVDLLRRLAELRGEEIAPPDKGVFSKLRSAFQ"
     gene            1228..1995
                     /locus_tag="VIJ44_05460"
     CDS             1228..1995
                     /locus_tag="VIJ44_05460"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HEY5165378.1"
                     /translation="MTRVAWCASADAVAHLIVEQLDDQLRVTDADGHHLQRVRRLRTG
                     ELITAADGAGQWREYTIAATEPGALTLEARAAPATEPVLHPPLRVACALTKGEHPDAV
                     VRHCSELGVDAVLLVIAARSVVRWDTDRATRALTRLRRVAREAAAQCRRARLLTVEPA
                     VPLADLARQPGLLVADRDGDPAHLLPAPTPAGWLVVVGPEGGLTPDEHTQLGHPPRLA
                     VGPHVLRAETSALAAAAALTGHRQPRPAPTPQSDHPA"
     gene            2109..2600
                     /locus_tag="VIJ44_05465"
     CDS             2109..2600
                     /locus_tag="VIJ44_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEY5165379.1"
                     /translation="MSLESEAGVQIGDRLRAIRRQQGLSLHDVEARSGQEFKASVLGA
                     YERGERALSVGRLLRLAELYDVPPDQLLPKGTDIEIDLTRAADDQRALTIDLVRLSQL
                     EEPDAEVLARYASTIQLQRQDFNGRMLTIRRDDVRVLAAVLGRSPDKLSDRLDELGLR
                     VAI"
     gene            2698..3600
                     /locus_tag="VIJ44_05470"
     CDS             2698..3600
                     /locus_tag="VIJ44_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012947814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEY5165380.1"
                     /translation="MVNLLGHRDELLKVIEASFPVTIHVRGNEITVTGDPDDADRVGR
                     LFEELVVLLEGGHALDIDGLGRSIEMLKADQRPTEVLGTEVLRGRKPVRPKTSGQKRF
                     VDAVAANTVTFAIGPAGTGKSYLAVALAVQALQTKSSNRIILTRPAVEAGERLGFLPG
                     DVLAKVDPYLRPLYDALYDMLDAEVVTRLMDRGTIEVAPLAFMRGRTLNDSFIILDEA
                     QNTTPEQMKMFLTRLGFGSKVVVTGDVTQIDLPEGRGRSGLQAVRTILDGIDGLAFVE
                     LDAHDVVRHRIVQDIVNAYERASG"
BASE COUNT          574 a         1557 c         1144 g          499 t
ORIGIN      
        1 gagcttgacc gccgccccgc cggcatcgtc ggcatcctcg gcccaacccg gatggactac
       61 cgccacgcgc tcgccgcggt caccgcggtg tccgagcagc tcggccggca cctctcatga
      121 cccgcgacca ctacgaggtg ctcggcgtcg accgtcacgc cagcgacgaa gacatcaaac
      181 gcgcctaccg tgacctcgcc cgccgctacc accccgattc gcaacccgac gcccccgacg
      241 ccgaagagcg cttcaaggag atcagcgtcg cgtacgaggc cttgcgcgac cccgaacggc
      301 gtcgccgtta cgacctcttc ggcgacaccg gcgccgcccg ctccggcggc ggcgagacgt
      361 tcgggttcgg tgacctcttc gacgccttct tcgccggcga cccgttcggg gcccgccgcg
      421 gccaggcggg cccgacccgc ggcgccgacg ccgaagtcgt cgtccagctc accctcaccg
      481 acaccatgtt cggaaagacc gagaccatcg acgcgtcgtt gccggtcagc tgtccccgct
      541 gcgaaggctc cggctgcgag cccggcaccc accccacccg ctgtgacgtg tgcggtggcg
      601 ccggcgaggt ccgccaggtc cgccggtcga tcctcggcca gatcgtcacc gccgcaccgt
      661 gcggggtctg cgaggcgacc ggccgccgca tcctcagcca gtgccgcgac tgccgcggcg
      721 acggacggat ccaggcccac cgcagcatcg acgtcgaagt ccccgccggg gtcggcgacg
      781 ggcaacgtct ccgcctcgcc ggacgcggcc ccgccgcgcc ccgtggcggg atccccggcg
      841 acctgtacgt caccgtccgc gtcgctgctc atcccgactt cgaacgccac ggcgacgatc
      901 tcctccatgt ccggcaagtc tcggtggcac aagccgcgct cggcacccgg ctcaccgtca
      961 ccggcctcga cgcggagcac gaggtgctcg tcccgcccgg cacccaaccc ggtcagctgt
     1021 tccgggtgaa acacgtcggg atcccgtcgc tgcgaggccg gggccgcggt gacctgctgg
     1081 tccgagtcga cgtcgaggtc cccacccgac tctcggagga agaagtcgat ctgctgcgcc
     1141 gcctcgccga gctgcgaggc gaggagatcg cacccccgga caagggcgtc ttctccaagc
     1201 tgcgctccgc gttccaatag ccgtccggtg acccgggtcg cctggtgcgc cagcgccgac
     1261 gccgtcgccc atctcatcgt cgagcaactc gacgaccagc tgcgcgtcac cgacgccgac
     1321 gggcatcacc tccaacgggt ccgccggctc cgaaccggag agctgatcac cgccgccgac
     1381 ggcgccggcc agtggcgcga gtacacgatc gccgccaccg agcccggggc cctcaccctc
     1441 gaggcccgag ccgcgcccgc caccgaaccg gtcctgcacc cgccgctgcg cgtcgcgtgc
     1501 gccctcacca aaggggaaca ccccgacgcg gtcgtccgcc actgcagcga gctcggtgtc
     1561 gacgcggtgc tcctcgtcat cgccgcccgc tcggtcgtcc gctgggacac cgaccgggcc
     1621 acccgagccc tgacccggct ccgccgcgtg gcccgcgagg ccgccgccca gtgccgccgg
     1681 gcccgcctcc tcaccgtcga gcccgcggtg cccctcgccg acctcgcccg ccagcccggc
     1741 ctcctcgtcg ccgaccggga cggtgaccct gcccacctcc ttcccgcccc gacccccgcg
     1801 ggctggctgg tggtcgtcgg cccggaaggc ggcctgaccc ccgacgagca cacccaactc
     1861 gggcatccgc cccgcctcgc cgtcggcccc cacgtcctgc gggccgagac ctcggccctc
     1921 gccgccgccg ctgccctcac cggccaccgc cagccccgac ccgcccccac cccccagtcc
     1981 gaccacccag cgtgacgccg ccggccatct ttggtacgct ccgcgcggtt cctgggcgtg
     2041 gttccgggcc aacatggggc agttgaaggg gctggtaggc aatggctggg gcaaatgggg
     2101 aacatccggt gagtctcgag agtgaggccg gtgtacagat cggcgaccgg ctgcgggcca
     2161 tccgccgcca gcagggcctg tccctgcatg acgtcgaggc ccgctcaggc caggagttca
     2221 aggcgtcggt cctcggcgcc tacgagcggg gcgaacgggc cctctcggtc ggccgcctgc
     2281 tgcgcctcgc cgagctctac gacgtgcccc cggaccagct cctgcccaag ggcaccgaca
     2341 tcgagatcga cctcacccga gccgccgatg accaacgggc cctcaccatc gacctggtcc
     2401 ggctgagcca gctcgaggag cccgacgccg aggtcctggc ccgctacgcc tccaccatcc
     2461 agctgcaacg ccaggacttc aacggtcgca tgctcaccat ccgccgcgac gacgtccggg
     2521 tcctcgccgc cgtcctgggt cggtccccgg acaagctcag cgaccgtctc gacgagctcg
     2581 gcttgcgcgt cgcgatctga aacgcgccgg gcggcgagac ccggcgctaa cctgcggcgc
     2641 gaggtgtcca cgccgtcgcc cacgcagctc aaagtcctcg tcccggggaa ccacctcatg
     2701 gtgaacctgc tcgggcatcg cgacgagctc ctcaaggtca tcgaggcgtc cttccccgtc
     2761 acgatccacg tgcgcggcaa cgagatcacc gtcaccggtg accccgacga cgccgaccgg
     2821 gtcggtcgcc tcttcgaaga gctggttgtg ctcctcgaag gtggccacgc cctcgacatc
     2881 gacgggctcg gacgatccat cgagatgctc aaagccgacc agcgacctac cgaagtgctc
     2941 ggcaccgaag tcctgcgggg ccgaaaaccg gtccgcccga agaccagcgg gcagaaacgc
     3001 ttcgtcgacg cggtggccgc caacaccgtc accttcgcca tcggccccgc cgggaccggc
     3061 aagagctacc tcgccgtggc cctcgcggtg caagccctcc aaaccaagtc cagcaaccgc
     3121 atcattctca cccgccccgc cgtcgaagcc ggtgaacgac tcgggttcct acccggcgac
     3181 gtcctcgcca aagtcgaccc gtacctgcgg cccctctacg acgcgctcta cgacatgctc
     3241 gacgccgaag tcgtcacccg cctcatggat cgtggcacca tcgaggtcgc acccctcgcc
     3301 tttatgcgcg gccgaaccct caacgactcg ttcatcatcc tcgacgaagc ccagaacacc
     3361 acccccgagc agatgaagat gttcctcacc cgtctcgggt tcggctccaa agtcgtcgtt
     3421 accggcgacg tcacccagat cgacctcccc gaaggccgcg gccgctccgg cctccaagcc
     3481 gtccgcacca tcctcgacgg catcgacggg ctggccttcg tcgagctcga cgcccacgac
     3541 gtcgtccgcc accgcatcgt ccaagacatc gtgaacgcct acgagcgggc cagcgggtga
     3601 ggccccgacc accccggcga cccccgggcg cgccgtgacc ccgtcggcac cggtccaggt
     3661 cagcggctac gacgagcaag acgccgtcac catcgagatc ccccgttggg tgcgactcgc
     3721 ccaacttgtc ctccacgccg aacggatcga ccccgacgcc gaactcggcc tcat
//
LOCUS       DATCCH010000284         4746 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_321432, whole genome shotgun sequence.
ACCESSION   DATCCH010000284 DATCCH010000000
VERSION     DATCCH010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4746
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321432"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="VIJ44_05475"
     CDS             complement(<1..591)
                     /locus_tag="VIJ44_05475"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5165381.1"
                     /translation="MPTVVAAGFLDNVWTFLTDGSHWQGSEGIPHLALQHLQLTVVSG
                     VVAAAIALPIGIGLGHLRKAGALAINVANIGRALPALALLILAVQWFGIGTPTGILAL
                     VHSLPAFIAMVALAVPPMVANSYVGVSGVDEEVREAARGMGMNGRQVLWRVELPMALP
                     LIMAGVRTAIVAIVATATLAAYVNGGGLGTLISVGFA"
     gene            complement(591..1295)
                     /locus_tag="VIJ44_05480"
     CDS             complement(591..1295)
                     /locus_tag="VIJ44_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019952099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5165382.1"
                     /translation="MVELGLSTVVARLLGEDIGPKWLYWPWVSDHLSEIQDRLTQHIQ
                     LTLLSVGFGLLIAFPLALLSVRYRRVYGPVLGVTGILYTIPSLALFALLLPVTGLSAA
                     TAVIPLTAYTLLILIRNMVTGLHGVPPEVRDAAEGMGYSPTGRLLRVELPLALPAIFA
                     GIRIATVTTIGLVTVTALIGQGGLGQLLLDGLNRDFRTPLTVGIVLSLALAVVADLFL
                     VGMLRASTRWQRKARA"
     gene            complement(1308..2471)
                     /locus_tag="VIJ44_05485"
     CDS             complement(1308..2471)
                     /locus_tag="VIJ44_05485"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEY5165383.1"
                     /translation="MRLALNLEQLLHRPPGGIGRYTAELARLLPGPDPDGGERIEVIP
                     FVARHRWSKIRPAFGAFGLADLEPVVLLLPRPVLYDTWNVFGLPPLGMLHPMLRGVDL
                     VHAPSLAVPPRSGAPLIVTAHDAATLVAPETYPRRGRKFHNRGFGAAARRADLVIAPT
                     NAAAEEILARTAIGPDRIRIVPHGVAHRQVSQGIVEATRATLGLGDEPYVLWVGTLEP
                     RKNLPLLLDAFRAVVRAGLPERLVIVGPDGWRGGPRAVAAAAEDLGDRVRFTGAIRAD
                     RLTALYRGASLFAFPSIHEGFGLPVLEAMAQGVAVVCSDIPVLREVGGAAARFAPPTD
                     AGAWGETMVALLSDETERARMGAAALAWSAEFTWERCIRRTRRCYREVLGSAR"
     gene            2470..3618
                     /locus_tag="VIJ44_05490"
     CDS             2470..3618
                     /locus_tag="VIJ44_05490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY5165384.1"
                     /translation="MIKLCSIQPVAERGGSDQALLRMVRSLPRPEFECHVVVPAEPPL
                     RGDFEAAGVEVHVLPMRRISRSHGLRDWVGYALAWPFTVIRLARLVRRLNVDVVHTNS
                     FHSWYGWAAALLAHRPHVWHGREIVVQSNAALAVERFLIPRFSARVLSMSHAIADQLD
                     GVDVVVVHETPDPHEFSPERAGRFRARIPIDDGVLLVGAAGRIDSWKGVDVLLDAFER
                     VRASRGDVELVVAGGPVRGKEPLFESLAATARAIPGVHWLGARDDIAELLADLDLFVL
                     PSTEPEPYGLVLVEALMSGAPVVATDAGGPREIVTEATPGSGQLVPVADRAALAAAVL
                     AALEGRRETTAALRAARPSLRAAEPERFAAIFRAVVDSDTRKSGRSLR"
     gene            3647..>4746
                     /locus_tag="VIJ44_05495"
     CDS             3647..>4746
                     /locus_tag="VIJ44_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018489350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HEY5165385.1"
                     /translation="MIRLDSVSKRFSNGQVAVDALTLEIPAGETCVLVGPSGCGKTTT
                     LKMINRLIEPTSGRIFLDDEDVTRADPVRLRRRIGYVIQQVGLFPHQNIGTNVGTVPR
                     LLGWKKDRIVQRVDELLELVGLAPGEYRDRYPSQLSGGQRQRVGVARALAADPPVLLM
                     DEPFGAIDPVTRMRLQDEFLHLQSEVRKTVVFVTHDIEEAVKMGDRICILEVGGKLAQ
                     YDTPAEVLGSPASPFVADFVGADRGLKRLRVTPIARDTLEHPPTVLPSASLADARAAI
                     ERSGADWVAVVSSDGRLVGHVRRDRSHGDGPVSSCTEHLEAWVSIDAPLEDALASMLL
                     TDLGWVAVLDGDRFLGVLTPESVYLTLRRSLDG"
BASE COUNT          744 a         1705 c         1640 g          657 t
ORIGIN      
        1 cgcgaagccg accgagatga gcgtcccgag gccgccgccg ttgacgtagg cggcgagagt
       61 ggcggtcgcc acgatggcga cgatcgcggt gcgcacaccg gccatgatca gcggcagcgc
      121 catcgggagc tcgactcgcc acagcacctg gcggccgttc atgcccatcc cgcgtgcggc
      181 ttcccggacc tcctcgtcga cccccgagac acccacgtag gagttcgcga ccatcggggg
      241 gacggcgagg gcgaccatcg cgatgaacgc cggcagcgag tgcaccagcg ccaggatccc
      301 ggtcggtgtg ccgatcccga accactggac cgcgaggatc agcagggcga gcgcgggcag
      361 cgcccgaccg atgttcgcca cgttgatcgc caacgccccc gcctttcgga gatggccgag
      421 cccgatgccg atcggcagcg cgatcgccgc cgcgacgaca ccggagacga ccgtcagctg
      481 gaggtgctgc agcgcgaggt gggggatccc ttcgctgccc tgccagtgcg agccatcggt
      541 caggaacgtc cacacgttgt cgaggaagcc cgcggccacg accgtcggca tcacgcccga
      601 gcctttcgct gccaccgggt cgaagcccgc aacatgccga ccaggaacag gtcggcgacg
      661 accgcgaggg ccagcgagag cacgatcccg accgtcagcg gggtgcggaa gtcgcggttc
      721 agcccgtcga ggaggagctg gcccaggccg ccttgaccga tgagcgccgt cacggtcacg
      781 agcccgatgg tggtgaccgt cgcgattcgg atccccgcga agatcgccgg gagcgccagc
      841 ggcagctcga cccggaggag ccgcccggtc ggcgagtacc ccatcccttc agcggcgtca
      901 cgcacctccg ggggcacgcc gtgcagcccg gtcaccatgt tccggatgag gatcaacaac
      961 gtgtaggcgg tcaacgggat caccgccgtg gcggccgaga ggcccgtgac cggcagcagc
     1021 agcgcgaaca gcgcgaggga cgggatcgtg tacaggatcc cggtgacgcc gagaaccggt
     1081 ccgtacaccc gccggtagcg gacggagagc aacgccaacg gaaacgcgat gagcagcccg
     1141 aagccgaccg agagcagcgt caactggatg tgctgcgtga gccggtcctg gatctcgctc
     1201 agatgatcgg acacccaggg ccagtacagc cacttcggac cgatgtcctc gccgagcaga
     1261 cgggcgacca ccgtcgagag gccaagctcc accacgccgc gacgaggtta ccgagccgag
     1321 ccgagcactt ctcggtagca acgtcgggtt cggcggatgc accgttccca ggtgaactcg
     1381 gccgaccagg caagcgccgc ggcccccatc cgggctcgtt cggtctcgtc gctcagcaac
     1441 gcaaccatgg tctcccccca cgccccggcg tccgtgggtg gcgcgaaccg ggcggcggcg
     1501 ccgcccacct cgcggagcac cgggatgtcg gagcagacga ccgcgacgcc ttgggccatc
     1561 gcctcgagca cggggagccc gaagccttcg tggatgctcg ggaaggcgaa cagcgacgcc
     1621 ccgcgataga gcgccgtgag ccggtcggcg cggatcgcgc cggtgaaccg gacccgatcg
     1681 ccgaggtcct cggcggctgc ggcgacggcg cgcggaccgc cacgccaacc gtcgggcccc
     1741 acgatcacca gccgctccgg gagacccgct cgcaccacgg cgcggaacgc gtcgaggagc
     1801 agcggcaggt tcttcctcgg ctccagcgtt ccgacccaca gcacgtacgg ctcgtcgccc
     1861 agccccagtg tggcccgggt ggcttcgacg atcccttggc tgacctggcg gtgcgcgaca
     1921 ccgtgcggga cgatgcggat ccggtcggga ccgatcgcgg ttcgggccag gatctcctcg
     1981 gccgcggcgt tggtgggagc gatcaccaga tcggcccgac gggcggcggc accgaagccc
     2041 cggttgtgga atttccggcc ccggcgaggg taggtctcag gagcgacaag cgtggccgcg
     2101 tcgtgcgcgg tcacgatcaa cggtgcaccg gaccgcggtg gaaccgccag cgaaggggca
     2161 tggaccagat ccacgcctcg caacatgggg tgcagcatcc cgaggggcgg gaggccgaaa
     2221 acgttccaag tgtcgtagag caccgggcga ggaagcagca acaccaccgg ttcgaggtcg
     2281 gcgagaccga acgcgccgaa cgcgggcctg atcttggacc agcggtgccg cgccacgaac
     2341 gggatcacct cgatacgctc gccgccgtcc gggtcgggcc caggcaggag ccgggcgagt
     2401 tcggcggtgt agcggccgat cccgcccggt gggcgatgga gcagctgctc gaggttgagg
     2461 gccaggcgca tgatcaagct ctgctcgatc caaccagtcg ctgagcgcgg cgggtcggat
     2521 caggcgctgc tacggatggt ccgcagcctg ccacgccccg aattcgaatg ccatgtcgtc
     2581 gtccccgccg agccgccgct acgcggcgac ttcgaggccg ccggcgtcga ggtccatgtc
     2641 ctgccgatgc gccgaatctc tcgcagccat gggctgcgcg actgggtcgg gtacgcgctg
     2701 gcgtggccgt tcacggtgat ccgcctggcc cgcctcgtcc gccgtctcaa cgtcgacgtg
     2761 gtgcacacca actcgtttca ctcgtggtac gggtgggccg cggcgcttct cgcccaccgt
     2821 ccccacgtct ggcacggacg agagatcgtg gtgcagtcca acgccgcgct cgcggtcgaa
     2881 cgctttttga tcccgcgttt ctcggcccgg gtgctgtcca tgtcgcacgc catcgccgac
     2941 cagctcgacg gcgtcgacgt cgtcgtcgtg cacgagaccc ctgacccgca cgaattcagc
     3001 cccgaacgcg cggggcgctt ccgggcgcga atccccatcg acgacggggt gctgctcgtc
     3061 ggtgccgcgg gtcgcatcga ctcctggaag ggcgtcgacg tcctcctcga cgcgttcgag
     3121 cgggttcggg cctcgcgggg cgacgtggag ctcgtggtgg ccggcggccc cgtgcgcggc
     3181 aaggaacccc tcttcgaatc cctcgccgcg acagcgcgtg ccattcccgg ggtgcactgg
     3241 ctcggcgccc gagacgacat cgcggagctc ctcgccgatc tcgacttgtt cgtcttgccg
     3301 tcgaccgaac ccgagccgta cggcctggtg ctcgtcgagg cgctgatgag cggcgccccg
     3361 gtggtcgcca ccgacgccgg cggaccccgc gagatcgtca ccgaggccac acccgggagc
     3421 ggccagctgg tacccgtggc ggaccgcgcg gcactggctg ccgcggtcct cgccgcgctc
     3481 gaagggcgac gcgagacgac cgcggccctc cgagcagcgc gcccgtcgct tcgcgccgcc
     3541 gagccggagc ggttcgccgc gatcttccgt gcggtcgtcg actccgacac gcgtaagagc
     3601 ggccggtcgc tgcgctaatg tcgtggaccg ctccggaggc ccccgcatga tccggctgga
     3661 ttctgtctcg aagcgcttct cgaacggtca ggtcgcggtc gatgcgttga cgctcgagat
     3721 accggccggc gagacctgcg tcctggtggg gccgtccggt tgcggcaaga cgacgacctt
     3781 gaagatgatc aaccgcctga tcgagccgac gagcggccgc atcttcctcg acgacgagga
     3841 cgtcacccgc gccgacccgg tccggcttcg ccgacggatc ggctacgtca tccaacaggt
     3901 cggcctgttc ccacatcaga acatcgggac gaacgtcggg accgtccccc gcctattggg
     3961 ctggaagaaa gaccgaatcg tgcagcgggt agacgagctc ctcgagctcg tgggtctcgc
     4021 acccggcgag taccgcgacc ggtacccgtc gcagctctcc ggcgggcagc gccaacgggt
     4081 cggcgtcgcc cgagccctgg ccgccgatcc tcccgtcctg ctcatggacg agcccttcgg
     4141 cgcgatcgat cccgtcacgc ggatgcgtct ccaagacgag ttcctgcacc tccagagcga
     4201 ggtgcgaaag accgtggtgt tcgtgaccca cgacatcgag gaggcggtca agatgggcga
     4261 ccgcatctgc atcctcgagg tcggcggcaa gctcgcgcag tacgacacgc cggccgaggt
     4321 gctcggcagc cccgcctccc cgttcgtcgc tgacttcgtc ggcgcggacc gcggattgaa
     4381 gcggctgcgg gtcacgccca tcgcccggga cacgctcgag catccaccca ccgtgctgcc
     4441 gagcgcgtcg ctggccgacg cccgcgccgc catcgaacgc agcggcgccg actgggtcgc
     4501 cgtcgtcagc agcgacggtc gcctcgtcgg tcatgtccgc cgcgaccggt cccacgggga
     4561 cgggcccgtc tcgtcctgca ccgaacacct tgaggcctgg gtctcgatcg acgccccgct
     4621 cgaggacgcg ctggcgtcca tgctgctcac cgacctcggc tgggtggccg tgctcgacgg
     4681 ggaccggttc cttggcgtcc tgacccccga atcggtctac ctcacgctcc gccgatcact
     4741 ggacgg
//
LOCUS       DATCCH010000285         2171 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_56672, whole genome shotgun sequence.
ACCESSION   DATCCH010000285 DATCCH010000000
VERSION     DATCCH010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56672"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VIJ44_05500"
     CDS             complement(<1..198)
                     /locus_tag="VIJ44_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018523226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165386.1"
                     /translation="MDRPPVFADRADAGRRLADALRRPPAAPTAPTAPVVCGVPRGGV
                     VVAAEVAAALGSPLRAIVARKV"
     gene            235..471
                     /locus_tag="VIJ44_05505"
     CDS             235..471
                     /locus_tag="VIJ44_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020553282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEY5165387.1"
                     /translation="MAISAYVLIQTEVGKAVKVANAVRTIDGVVAADDVTGPYDVIVR
                     TEAPSLDDLGRMVVSQIQAVEGITRTFTCPVVNL"
     gene            complement(468..1601)
                     /locus_tag="VIJ44_05510"
     CDS             complement(468..1601)
                     /locus_tag="VIJ44_05510"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018180357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HEY5165388.1"
                     /translation="MTRIRVLLLFGGRSAEHDVSRVSAAAVAAALDPTRYEVVPVAIT
                     TAGQWLLAGEARAALEGARDALPTEFRVEGQPVVEVPDPSQPALVPLDATAPGAAVPT
                     DVDVVLPLLHGPYGEDGTVQGLFELAGLAYVGAGVIGSALAMDKVAMKRAFAAAGLPV
                     ARWLAHREGHPADEFADQVEAQLGLPSFVKPANMGSSVGVSKATDRAALDTAIERALA
                     FDEWVIAEEAITGREIEVGILGDDPPEASVPGEIVPGDEFYSYADKYEDDAVQLLTPA
                     PLTAEQADEVRGLAVRAFEACRGEGMARVDFFFTEDRGFLVNEVNTIPGFTPVSMYPR
                     LWAESGVSYPRLIDQLIGLALDRHQRRSRRAGRQRDASDDPRT"
     gene            complement(1598..1966)
                     /locus_tag="VIJ44_05515"
     CDS             complement(1598..1966)
                     /locus_tag="VIJ44_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165389.1"
                     /translation="MHALLDFHKIWGYVAIAANGLVGVAALVAWRNPRLRGRWLWISI
                     LVAEAAILLQVVVGVILQASKQYKAPNFHIFYGVLAFLTVGLAYQYRLQMRGRRELLY
                     GLVGLFIMGLGIRAVLQVTG"
     gene            complement(1992..>2171)
                     /locus_tag="VIJ44_05520"
     CDS             complement(1992..>2171)
                     /locus_tag="VIJ44_05520"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEY5165390.1"
                     /translation="SDERDVYLVYTRWRSQADFERWAASPAFQHGHRAHATDGPVSSE
                     SALWSFDVVQHEEGG"
BASE COUNT          325 a          775 c          793 g          278 t
ORIGIN      
        1 gaccttgcgg gccacgatcg cccgtagcgg cgagcccaac gcggccgcga cctcggccgc
       61 gaccacgacg ccgccgcggg ggacaccaca caccaccggg gcggtcgggg cggtcggggc
      121 cgccgggggg cgacggagcg cgtcagcgag gcgacgtccc gcgtcggcgc ggtcggcgaa
      181 gacgggcggg cggtccaccg ctacgatccg cgcaccacga cgaggggagt cggcatggcg
      241 atcagcgcgt acgtgctcat ccagaccgag gtcggcaaag ccgtcaaggt cgcgaacgcc
      301 gtccggacca tcgatggcgt ggtcgccgct gacgacgtca ccggcccgta cgacgtcatc
      361 gtgcgcaccg aggcgccgag cctcgacgat ctcggccgga tggtcgtctc ccagatccag
      421 gccgtggagg gcatcacccg cacgttcacc tgtccggtcg tcaacctcta ggtccgcggg
      481 tcgtcgctgg cgtcccgctg gcggccggcg cggcggctcc gccgctggtg tcggtcgagg
      541 gccaagccga tgagctggtc gatgaggcgg gggtacgaga cgccgctctc ggcccagagc
      601 cgcgggtaca tcgacacggg ggtgaacccg gggatggtgt tcacctcgtt cacgaggaac
      661 ccgcggtcct cagtgaagaa gaagtccacc cgggccatgc cctcgccccg acacgcttcg
      721 aacgcccgca ccgcgaggcc gcggacctcg tcggcttgct cggccgtgag gggcgccggg
      781 gtgaggagct gcaccgcgtc gtcttcgtac ttgtcggcgt agctgtagaa ctcgtcgccg
      841 ggcacgatct cgccggggac ggacgcctcg gggggatcgt caccgaggat ccccacctcg
      901 atctcgcgtc cggtgatggc ctcctcggcg atcacccact cgtcgaaggc gagggcccgc
      961 tcgatcgcgg tgtcgagcgc ggcccggtcg gtggccttcg acactccgac cgaggacccc
     1021 atgttggcgg gcttcacgaa ggagggcagg ccgagctggg cctcgacctg gtcggcgaac
     1081 tcgtcggccg ggtggccctc acggtgcgcc agccaccggg cgacggggag ccccgcggcg
     1141 gcgaaggccc gtttcatcgc gaccttgtcc atcgccagcg cggagccgat cacgccggca
     1201 ccgacgtacg ccaggccggc gagctcgaag aggccttgca ccgtgccgtc ttccccgtag
     1261 ggcccgtgca ggagcggcag gacgacgtcg acgtcggtgg ggaccgccgc gccgggcgcg
     1321 gtggcatcga ggggcacgag cgcgggctgg gacgggtccg ggacctcgac caccggctgg
     1381 ccctccacgc ggaactcggt cggcaacgcg tcgcgcgccc cctcgagcgc ggcgcgagcc
     1441 tcgccggcga gcagccactg gccggcggtg gtgatcgcga ccgggacgac ctcgtaccgg
     1501 gtggggtcga gcgccgcggc gaccgccgcg gcgctgaccc gcgacacgtc atgctcggcg
     1561 gagcgtcccc cgaacagcaa cagcacccgg atgcgggtca tccggtgacc tggagcacag
     1621 cccggattcc caatcccatg atgaacaggc cgacgaggcc atagagcagc tcgcggcggc
     1681 cccgcatctg cagccggtac tggtacgcca ggcccacggt gaggaaggcg aggacgccgt
     1741 agaagatgtg gaagttcggc gccttgtact gcttgctcgc ctggaggatc acgcccacca
     1801 cgacctggag caggatcgcg gcctcggcga cgaggatcga gatccacagc caccggccgc
     1861 gcagccgcgg gttccgccag gcgacgagcg cggcgacgcc cacgagaccg ttggccgcga
     1921 tggcgacata gccccagatc ttgtggaagt cgaggagggc gtgcacgaca gttgacgcgc
     1981 ccggcgggtc gctacccgcc ctcttcgtgc tggacgacgt cgaaggacca cagcgcgctc
     2041 tcgctgctca ccggcccgtc ggtcgcgtgc gcccggtgcc cgtgctgaaa cgccgggctc
     2101 gccgcccacc gctcgaagtc cgcttgcgaa cgccaccgcg tgtagacgag gtagacgtcg
     2161 cgctcgtcgc t
//
LOCUS       DATCCH010000286         2065 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_529914, whole genome shotgun sequence.
ACCESSION   DATCCH010000286 DATCCH010000000
VERSION     DATCCH010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529914"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VIJ44_05525"
     CDS             <1..221
                     /locus_tag="VIJ44_05525"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEY5165391.1"
                     /translation="FFAGQNLMLGALALGLGTCPIGLARPLLSQADVKDELGVPPAMV
                     AALPIVVGVPDEQPVAHPRRPPRSLYRR"
     gene            complement(289..1125)
                     /locus_tag="VIJ44_05530"
     CDS             complement(289..1125)
                     /locus_tag="VIJ44_05530"
                     /inference="COORDINATES: protein motif:HMM:NF004843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5165392.1"
                     /translation="MKQLTGKVAVVTGAASGIGLALARAFAAEGMRLVLADVEEDALG
                     EAVDGLARAGAEVLGVPTDVSLAESVEELAARAYECFRAAHLVCNNAGVATPGDPWEG
                     PLSLWEWTFAVNLWGVVHGIRAFLPMLLAQDEGHIVNTASAAGLRARPHLAPYVASKH
                     AVVGLSESLLHDLAQRPAHVGVSVLCPTWVRTRIVDGDRNWLPRFGAAPERSPDEQAL
                     HELASEALAGGADPEGVATAVVDAVRTGRFWVIPDPSVLDELQERARGAREGRNPSLP
                     RR"
     gene            complement(1456..>2065)
                     /locus_tag="VIJ44_05535"
     CDS             complement(1456..>2065)
                     /locus_tag="VIJ44_05535"
                     /inference="COORDINATES: protein motif:HMM:NF014042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase CofD family
                     protein"
                     /protein_id="HEY5165393.1"
                     /translation="ELARRMGVGTRLLPMTEAEVETRVLTATSERSFEEYLVRDAAGG
                     SVVGVEYRGIEGSTPAPGVLDAIAAANVVVLGPSNPISSLGPILGVSGMRAAVRDSPA
                     RVVGVTSVVANVPIRDPGEERRAQCRSALLASRGLPHTASAVAGLFADLLDVFVLDDA
                     DEGDAADAIRAGIEVRVVPTVVAGDAVAAGRLARVVLEAETP"
BASE COUNT          294 a          772 c          725 g          274 t
ORIGIN      
        1 tgttcttcgc tggtcagaac ctcatgctgg gtgctctcgc actcggcctg ggcacgtgcc
       61 ccatcgggct ggcgcgaccg ctgctgagcc aggccgacgt caaggacgag ctcggggtcc
      121 cacccgcgat ggtcgcggcg ctccccatcg tcgtcggggt ccccgatgag cagcccgtcg
      181 cgcacccgag acgaccgcct cggagcctct accggcgata aagcccggcg cagaccggtc
      241 gcccggcgga gcggtcgggc cgatcggtcg acgagcacct ggtgcgcgtc accggcgcgg
      301 gagagatggg ttgcggcctt cgcgcgcgcc tcgggcccgc tcctgcagct cgtcgaggac
      361 cgacgggtcg gggatcaccc agaagcgccc ggtgcgcacg gcatccacga cggcggtcgc
      421 gacgccctcg ggatcggctc cgcccgcgag cgcctcggac gcgagctcgt gcagcgcctg
      481 ctcgtcgggg ctgcgctcgg gcgcggcacc gaagcggggg agccagttgc ggtccccgtc
      541 gacgatgcgc gtccgcaccc aggtggggca cagcaccgag accccgacgt gggccgggcg
      601 ctgcgcgagg tcgtggagca gcgactcgct gagcccgacc accgcgtgct tcgacgcgac
      661 gtagggcgcg aggtggggcc gggcgcgaag ccccgcagcc gaggcggtgt tcacgatgtg
      721 gccctcgtcc tgggccagca gcatggggag gaaggcgcgg atcccgtgca cgacacccca
      781 caggttcacc gcgaacgtcc attcccataa ggagagtggc ccctcccacg ggtcgccggg
      841 cgtcgcgacc cccgcgttgt tgcagaccag gtgcgcggcc cggaagcact cgtacgcacg
      901 cgccgcgagc tcctcgaccg actcggccag cgatacgtcg gtcgggaccc ccagcacctc
      961 cgcgccggct cgggcgaggc cgtcgactgc ctccccgagg gcatcctctt ccacgtcggc
     1021 caacacgagg cgcatgccct cggccgcgaa cgcgcgcgcg agcgcgagac caatcccgct
     1081 ggcggcgccg gtgaccacgg cgaccttccc cgtgagctgc ttcaccggca accgctccta
     1141 acgcccgacg tcactgtggg ccatggacga cacgtcggga ccaggcgggg tgaggctctc
     1201 ggccggggcg accgccgcgt gcaggagcgt acgtggaccg atcatcttga tatcgacatc
     1261 gagcgcgaga tgaggtccgg tcaggatgag tcgggtccct cggaggcagg aaatgcggta
     1321 ttcctccccc gagccgtttc gagcgagtgg cggatcggtg atcacggtcg cggcggggac
     1381 ggtgctgcgc ggcgaggcct cgcccccgtc ggcggacgac tgattgccgc gagtcctgcc
     1441 cgaacgtgtg tgcgctcacg gggtctccgc ttccaacacg acccgggcga gtcgccctgc
     1501 ggcgaccgcg tcaccggcga cgacggtcgg cacgacccga acctcgatgc cggcgcggat
     1561 cgcgtcggcg gcgtcgccct cgtcggcgtc gtcaaggacg aacacgtcga gcagatccgc
     1621 gaacagcccc gccactgcgc tcgccgtgtg gggaagcccg cgggaggcga gcagtgcgct
     1681 gcggcactgt gcgcggcgct cctcacccgg atctcgtatc ggtacgttcg ccacgacgga
     1741 cgtcacgccg acgacccgcg ccggtgagtc gcgcacggct gcccgcattc cggacacccc
     1801 caggatcggt ccgaggctcg agatcgggtt gctcggtccg agcaccacca cgttggccgc
     1861 cgcgatcgcg tccagcacgc ccggcgccgg cgtcgatccc tcgatgcccc ggtactcgac
     1921 tcccacgacc gatccacccg ccgcgtcacg caccaggtac tcctcgaagg accgctcact
     1981 cgtcgcggtc agcacccggg tctcgacctc ggcctccgtc atcgggagca ggcgggtccc
     2041 cacgcccatc cgccgagcga gctcg
//
LOCUS       DATCCH010000287         2278 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_321643, whole genome shotgun sequence.
ACCESSION   DATCCH010000287 DATCCH010000000
VERSION     DATCCH010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321643"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="VIJ44_05540"
     CDS             complement(<1..268)
                     /locus_tag="VIJ44_05540"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEY5165394.1"
                     /translation="MDDTASRRSPSTFVISITPFTDDGALDEPGLRAHLSRLGDAGIG
                     VYLAGSGSGEGYTLDDGEVARVLAIGAETLGGRVPVRAMGVEPRT"
     gene            316..1449
                     /locus_tag="VIJ44_05545"
     CDS             316..1449
                     /locus_tag="VIJ44_05545"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5165395.1"
                     /translation="MTKRGRHVTTQPGRRYRLISADSHVNEPPDLWTNRVPAAMRDRA
                     PRIERFDEGDAWVIEGVADPINFGMNACAGLEPEAMRGWVRFEDIRRGGHDPAARLDE
                     MDRDGVDAEVLYPTPRLAQAVFANPDAEYRAAMVAAYNDWIAEYVEYAPERFGGLAML
                     PNWGAEAAVAELDRALGRPGMRGVVMGTYPNGSLQPDPSDDKVWGRLAEQRIPLGIHV
                     SLATRMPTAHAAKLPGYGRFFDAPNRMIDMIFAGIFDRFPDLDVVFAEVDFGWVPYAK
                     EQIDNNYHRLEPVSRFGLAANPSEYIERHFHFGFMTDTFGIQNRHHVGVERVLWSNDY
                     PHISADWPASWRTIQAAFSGVPPEERHLILHGNSQRLYGFGRP"
     gene            1563..>2278
                     /locus_tag="VIJ44_05550"
     CDS             1563..>2278
                     /locus_tag="VIJ44_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016889681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY5165396.1"
                     /translation="MAIATINPATGETVKTFDALSDTEIEDRLERAHTAFARYRRTSF
                     EDRSGWMTRAAEILDRDRDDVARVMTTEMGKTLIAARAETAKCATACRFYAEHAPGFL
                     ADEPVDPQSVAAEQAFARYQPLGPVLAVMPWNFPLWQAMRFAAPALMAGNVGLLKHAS
                     NVPHTALFLQDLFHEAGFPDGVFQTLLIGSDQVERVIRDDRVVAATLTGSAPAGRSVA
                     AIAGDAIKPTVLELGGSDAF"
BASE COUNT          381 a          860 c          714 g          323 t
ORIGIN      
        1 cggtgcgtgg ctcaaccccc atcgcccgga ccgggacgcg acctccgagc gtctccgcgc
       61 cgatggcgag aaccctcgcc acttcaccgt cgtcgagcgt gtagccctca ccgctgccac
      121 tcccggcgag gtacaccccg atcccggcgt cgccgagccg ggacagatgg gctcgcagac
      181 ccggctcgtc gagcgcaccg tcgtccgtga acggcgtgat gctgatcacg aacgtgctcg
      241 gcgaacgtcg actcgcggtg tcgtccatgg ggccgccgga gtctacggac catccgggta
      301 cgatcacgtc cctcgatgac caaacgggga cggcacgtga ccacgcaacc tggacgccgg
      361 tatcggctca tctcggccga cagccacgtc aacgaacccc cggacctgtg gaccaaccgg
      421 gtcccggccg cgatgcggga ccgggcgcct cgcatcgaac gcttcgacga aggcgacgcc
      481 tgggtcatcg aaggcgtcgc ggaccccatc aacttcggga tgaacgcctg cgcgggcctc
      541 gaacccgagg cgatgcgcgg ctgggtgcgc ttcgaggaca tccgacgcgg cggccacgac
      601 cccgcggccc gcctcgacga gatggaccgc gacggcgtcg acgccgaggt gctctacccc
      661 accccccgcc tcgcccaggc cgtcttcgcc aaccccgacg ccgagtaccg cgccgcgatg
      721 gtcgcggcgt acaacgactg gatcgccgag tacgtcgagt acgccccgga acgcttcggc
      781 ggcctcgcca tgcttccgaa ctggggtgcc gaagccgcgg tcgccgaact cgaccgggcc
      841 ctcggccggc ccgggatgcg cggcgtcgtc atgggcacct acccgaacgg gtcactgcaa
      901 cccgacccga gcgatgacaa ggtgtggggc cgactcgcgg agcagcgaat cccgctcggc
      961 atccatgtca gcctcgcgac ccggatgccg accgcgcacg ctgcgaagct ccccggctac
     1021 gggcggttct tcgacgcccc gaaccggatg atcgacatga tcttcgccgg gatcttcgac
     1081 cggttccccg acctcgacgt cgtcttcgcc gaagtcgact tcggttgggt tccgtacgcg
     1141 aaggaacaga tcgacaacaa ctaccaccgc ctcgaacctg tcagccggtt cggtcttgcc
     1201 gcgaacccga gcgagtacat cgaacggcac ttccacttcg ggttcatgac cgacacgttc
     1261 ggcatccaga accgtcacca tgtcggtgtc gaacgggtgc tgtggtccaa cgactaccca
     1321 cacatcagcg ccgattggcc cgcgtcatgg cgcaccatcc aagccgcgtt ctccggcgtc
     1381 ccacccgagg aacgccacct gatcctgcac gggaacagcc agcgcctcta cggcttcggc
     1441 cgcccgtaac ctcggctccc acacaccgag cgcggccggt tgcgccgcgg gccccacgaa
     1501 gaagaatgag cgcgtcgacg cgacgtcgac ccctgcgccg atcccacgcg aggagcaccg
     1561 atatggcgat cgcaacgatc aaccccgcga ccggcgagac cgtcaagacg ttcgacgcct
     1621 tgtcggacac cgagattgag gatcgtctgg agcgggccca caccgccttc gcccggtatc
     1681 ggcggaccag cttcgaggac cggtcggggt ggatgacccg agccgccgag attctcgacc
     1741 gggaccgaga cgacgtggcc cgggtgatga cgaccgagat gggcaagacc ctcatcgcgg
     1801 cccgggccga gaccgcgaag tgcgcgaccg cctgtcgctt ctacgccgag cacgcccccg
     1861 gcttcctcgc cgacgagccc gtcgacccgc agtcggtggc cgcggagcag gccttcgccc
     1921 gctatcagcc cctcggcccg gtcctcgcgg tgatgccgtg gaacttcccg ctctggcagg
     1981 cgatgcggtt cgccgcaccg gcgctgatgg ccggcaacgt cgggctcctc aagcacgcct
     2041 cgaatgtccc ccataccgcc ctcttcctgc aggacctgtt ccacgaggcc gggttccccg
     2101 acggggtctt ccagaccctc ctgatcgggt cagaccaggt cgaacgggtc atccgtgacg
     2161 accgggtcgt cgccgcgacc ctcaccggca gcgcaccagc cggacggtcc gtcgcggcca
     2221 tcgctggcga cgcgatcaaa ccgacggtcc tcgagctcgg cggcagcgac gcgttcat
//
LOCUS       DATCCH010000288         2824 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_529976, whole genome shotgun sequence.
ACCESSION   DATCCH010000288 DATCCH010000000
VERSION     DATCCH010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529976"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            341..808
                     /locus_tag="VIJ44_05555"
     CDS             341..808
                     /locus_tag="VIJ44_05555"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEY5165397.1"
                     /translation="MVSSLLRTRVLVGVLFMVVLLTAGCMGPGRAGPPPPPAHGAASA
                     VAFVNSQIGKPYCFAGAGPSCFDCSGLTMRAWQAGGILLPHFSGAQYAMFPSVPLTQL
                     QPGDLVFPADPSQHVAMYVGNGMIVHATHTGDFVRAEPLSEPGLGLVLAVRPG"
     gene            complement(906..2138)
                     /locus_tag="VIJ44_05560"
     CDS             complement(906..2138)
                     /locus_tag="VIJ44_05560"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5165398.1"
                     /translation="MSPAGYLGDRRGTAATLRSIDTTAGGGFYAVVPEAETDSAAADL
                     ALGGTGVVIDVQTHLVRPSRAATTGAAALFGFLRMVDPERWGNGVDPSLLSAAEWAAC
                     VFGGSETAVALLTSPPGRAEDNVLTNDDIAAARAIVDRYAGARRVLTHTIVHPNLGPA
                     ELDAMVDWHDRLSPAGWKVYTLWSPPERGTSGGWYLDDDETGFPFLERVRELGPRIVC
                     AHKGIAGPVASLAPASASPRDVGPAASAFPDVTFVVYHSGYEPDVSEEGAHTDDRDRG
                     VSRLVTSLARAGVEPGANVYAELGSTWYLMLRRPREAAHVLGKLLLAVGEDRILWGTD
                     SVWYGAPQRLIDAFRAFRIPEWMQERFGYPALTETAKAKILGVNASRLYDVDLNTVAT
                     PDAGWLPAARDELGSRLA"
     gene            complement(2364..>2824)
                     /locus_tag="VIJ44_05565"
     CDS             complement(2364..>2824)
                     /locus_tag="VIJ44_05565"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEY5165399.1"
                     /translation="AAAAGTTDTASNVGFAIPIDAADAIIQQIQGGHESTNEHLGATA
                     FLGVRLQPDDTGNGPGLGGGGSRGGGGGSAVGSGAVIQSVNAGSPANAAGLGAGDTIT
                     SFDGQPVSSAATLTQLVQAKHPGDTVTIEWTDSSGQQHSATARLTSGPAA"
BASE COUNT          412 a          961 c          999 g          452 t
ORIGIN      
        1 gacgacggcg ggcgggcaag tcaaactcgg gtccacgaga tcgcgaccga actcgaatcg
       61 gccatcgagc cgcgttccgc ccagtaaggc tccttggcgg cgagccacgt tggtgtcgcg
      121 gcactgaccg ccggtgtgac cgcaaccccg ccctcaggga agcgaacgag ccgaggtcgc
      181 ccgctgtgaa ggggtcgtca ttgaccgagt tcggaagatg cggaccgtct gggtggaata
      241 cgggggctgt tttcggcttc agacaaggtt aggttcggcc gatcttgttc ctcagcggcg
      301 tcccccgtcg cgcccggaaa caggaggtcg gatgcgtacc gtggtttctt cgcttctgcg
      361 cacccgcgtg ctcgtcggcg tgctgttcat ggtggtgctg ctcaccgcag gttgcatggg
      421 accgggacgg gcgggaccgc cacctccgcc ggcacacggc gcggcgagcg cagtggcctt
      481 cgtgaacagt cagatcggga agccgtattg cttcgctggt gccggaccaa gctgcttcga
      541 ttgctcagga ctcaccatgc gggcgtggca ggcggggggg attttgcttc cgcacttctc
      601 cggtgcgcag tacgcgatgt tcccgtcggt accgttgacc cagttgcagc ccggcgacct
      661 ggtgtttcct gccgacccaa gccagcacgt cgctatgtac gtcggaaacg ggatgatcgt
      721 gcacgccacc cacaccggag atttcgtgcg ggccgagcca ctctcggagc cgggactcgg
      781 actcgtgctc gcggtccgcc ccggctgacc tcgcggctcg aagcggcgcc gagaccgagg
      841 cggtggcgac accgcggtga atcggacggt ggcgagggcg ggcccacctc gcggtctcac
      901 tcgggtcacg ccaggcgtga cccgagctcg tcccgggctg cggggagcca gccggcgtcc
      961 ggggttgcaa ccgtgttgag gtcgacgtcg tacagccgcg acgcgttcac gccgaggatc
     1021 ttcgctttcg cggtctcggt gagcgcgggg tagccaaacc gctcctgcat ccattccggg
     1081 atccgaaagg cgcggaacgc gtcgatgagg cgctgcggtg ctccgtacca gaccgaatcg
     1141 gttccccaca ggatgcggtc ctcaccgacc gcgaggagca gcttcccgag cacgtgggcc
     1201 gcctcccgcg ggcggcgcaa catgaggtac caggtggacc cgagctcggc gtagacgttc
     1261 gcgccgggct cgacgccggc ccgggccaga ctcgtcacca gtcgactcac cccccgatcc
     1321 cgatcgtcgg tgtgagcgcc ctcttcgctg acgtccggct cgtatccgga gtggtacacg
     1381 acgaacgtga cgtccgggaa cgccgaggcg gccggcccga cgtcgcgcgg tgacgcgctc
     1441 gccggagcga ggctcgcgac cggaccggcg atgcccttgt gggcgcagac gatgcgcggg
     1501 cctagctcgc gtacgcgttc caggaacggg aagccggtct cgtcgtcgtc caggtaccag
     1561 ccgccgctgg ttccgcgctc gggtggtgac cagagcgtgt acaccttcca accggcgggg
     1621 gagagccggt cgtgccagtc gaccatcgcg tcgagctcgg ccggacccag gttggggtgc
     1681 acgatcgtgt gtgtcagcac tcgccgcgct ccggcgtacc gatcaacgat cgctcgggcc
     1741 gccgcgatgt cgtcgttcgt cagcacgttg tcctcggcgc gccccggcgg cgacgtcagc
     1801 aaggccaccg cagtctcgct gcccccgaag acacacgctg cccactccgc cgccgacaac
     1861 aacgacgggt ccaccccgtt gccccatcgt tcggggtcga ccatccggag gaaaccgaac
     1921 agcgctgccg cgcccgtcgt cgcagcccgg ctcggtcgca ccagatgggt ttgcacgtcg
     1981 atcacgaccc cggtgccacc cagcgcgagg tcagccgccg ccgaatcggt ctcggcctcg
     2041 ggtactacgg cgtagaaccc tccacccgca gtggtgtcga tcgaacgaag cgtcgccgcg
     2101 gtgccccggc ggtctcccag ataacctgcc gggctcaacc ccaacgttga tgcgcgcgag
     2161 gtcgccgcgg cgcacgccag cgccgccgcc cgctgctcgg caggcccggg aggcaatggt
     2221 gcgtactcgt ccgatgagcg tcgcgcgagc agcggtggaa caagcggtgg agcagcgttc
     2281 agcgacaacg atgcgaacgg taacggctcg gcttgaccgg cgatctgtcg gcgagcggga
     2341 ctcccatcgc ccggtgggca gatctacgcg gcgggtcctg acgtgagtcg cgcggtggcc
     2401 gagtgctgct gtcctgagct gtcggtccat tcgatggtga cggtgtcgcc cggatgtttg
     2461 gcctgcacga gctgggtgag ggtcgcggcg gatgagaccg gctgcccgtc gaaggaggtg
     2521 atggtgtcgc ccgcgccgag gccggcggca ttggcgggac tgccggcgtt gacgctctgg
     2581 atgacggcac ccgacccgac cgcgctgcca ccgccaccgc cacgagagcc tccgcccccg
     2641 agcccgggtc cgttcccggt gtcgtcgggc tggagtcgca cacccaagaa cgcggtcgct
     2701 ccgaggtgct cgttggtgct ctcgtggccg ccctggatct gctggatgat ggcgtccgcg
     2761 gcgtcgatcg ggattgcgaa gccgacgttg gaggccgtgt cggtggtacc ggccgcggcg
     2821 gcgg
//
LOCUS       DATCCH010000289         2840 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_530162, whole genome shotgun sequence.
ACCESSION   DATCCH010000289 DATCCH010000000
VERSION     DATCCH010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530162"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..1312)
                     /locus_tag="VIJ44_05570"
     CDS             complement(149..1312)
                     /locus_tag="VIJ44_05570"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEY5165400.1"
                     /translation="MAHAQTPKSAPPTGGRRTRPPFRADHVGSLLRPKQLLAARDDLG
                     AGRIDAGQLRAIEDEAIRDAVRMQRDVGLQSATDGEFRRSSWHMDFIYQLGGIEKVEQ
                     DINVTFRNADGEVDFKPAAIRVTDKVALDHTIFAEDFQFLKGIVDDGITPKLTIPSPN
                     MVHYRAGRAALDPRAYIDIDQFWVDLSAAYAQQVSQIAALGCTYLQFDDTSLAYLNDP
                     RQRSALTERGEDTANLHETYVSNINNALAGRPEELVVTTHLCRGNFRSSWAAEGGYDF
                     VAETLFGSLDVDGFFLEYDDARSGGFEPLRFVAPGKMVVLGLVTSKRGELESHDELRR
                     RIDEASRYIPLEQLCLSPQCGFSSTVEGNAVSYDEQVAKLELVVEVATEIWGG"
     gene            complement(1315..1746)
                     /locus_tag="VIJ44_05575"
     CDS             complement(1315..1746)
                     /locus_tag="VIJ44_05575"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5165401.1"
                     /translation="MSTTPGVNALDHVLVLSDDIERSRDFYEGALGLHAGPRPPLEFD
                     GFWLYASERPCLHIAHRGSYRAHAQTLGLAVPERAGGPGPVDHIAFVAHDYDAVCQRL
                     ERCDIQPVRNDVPGGGPRQFFFFDPDGARVEINVPANDPEV"
     gene            complement(1743..2549)
                     /locus_tag="VIJ44_05580"
     CDS             complement(1743..2549)
                     /locus_tag="VIJ44_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5165402.1"
                     /translation="MSTPETPSAPASPDSPIVEGGLRLANLTVARGGRDVVHDITIDV
                     APGEVTALLGPNGAGKSSMVLAVGGVLPLTAGTVTLAGEQLAGKRPERIRRAGLAIVP
                     EGRRLLGELTVEDNLKIATYTLSAKAGAAARTHALELFPELEKRLHNQARALSGGEQQ
                     MVVLAQALVSQPKFIIIDELSLGLAPVVVQRLIPTIDTVTHNGIGVLLIEQFAAVALD
                     LANRAYVIEGGHIQYSGTAHQLKTPPELLHSAYLLRGDTTTPNESLPTTG"
     gene            complement(2546..>2840)
                     /locus_tag="VIJ44_05585"
     CDS             complement(2546..>2840)
                     /locus_tag="VIJ44_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007379945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5165403.1"
                     /translation="ELARAIVGKPRLVLLDEPAAGLPDEETTRMGQIITAIPERTGAL
                     TILVDHDMSLVSSCCQNTAVLDFGKLIASGPTDQVLRDPNVVKAYLGTENIAA"
BASE COUNT          431 a          819 c         1085 g          505 t
ORIGIN      
        1 atggccggtg aagaagtggt agtccgagcc gcagatgccg acggcgtcgg gcgcgacgac
       61 catctcgccg ggaccgggtt cgcccggctc gggccggtcc acgatcttca tcgatccaac
      121 gagctcagtg acgactgcga gcatcaggtc atcctcccca gatctcggtg gcgacctcga
      181 cgaccagctc gagcttggcg acctgctcgt cgtagctgac ggcgttgccc tcgaccgtgg
      241 aggagaagcc gcattggggc gagaggcaca gctgctcgag cgggatgtac cgggaggcct
      301 cgtcgatccg gcggcgcagc tcgtcgtggg actccagctc gccgcgtttg gaggtgacca
      361 gtcccagcac caccatcttg ccgggagcca cgaagcgcag cggctcgaag ccgccggagc
      421 gggcgtcgtc gtactcgaga aagaagccgt cgacatcaag cgatccgaac agcgtctcgg
      481 ccacgaagtc atacccaccc tcggccgccc acgaggagcg gaagttgccg cggcacaggt
      541 gcgtggtcac gacgagctcc tccggtcgtc cggcgagcgc attgttgatg ttggagacgt
      601 aggtctcgtg caggttcgcg gtgtcctcgc cgcgctcggt cagcgccgag cgctggcgcg
      661 ggtcgttgag ataggcgagg ctcgtgtcgt cgaactgcag gtaggtgcag cccagggcgg
      721 cgatctgcga gacctgctgc gcgtacgccg ccgacagatc gacccagaac tggtcgatgt
      781 cgatgtaggc gcgcgggtcc agcgcggcgc ggcccgctcg gtaatggacc atgttgggcg
      841 aggggatggt cagcttcggg gtgatcccgt catcgacgat gcccttcagg aactggaagt
      901 cctcggcaaa gatggtgtga tcgagcgcca ccttgtcggt gaccctgatc gccgccggct
      961 tgaagtcaac ctcgccgtcg gcgttgcgga acgtgacatt gatgtcctgc tcgaccttct
     1021 cgatcccgcc gagctggtag atgaagtcca tgtgccagga gctgcggcga aattcgccgt
     1081 cggttgccga ctgcagaccg acatcgcgct gcatcctgac cgcgtcgcga atggcctcgt
     1141 cctcgatcgc ccgcaactgg ccggcgtcga tcctcccggc gccaaggtca tcgcgggcgg
     1201 ctagcagctg cttgggccgc aacaggctgc cgacgtgatc ggcccggaag ggcgggcgcg
     1261 tgcgtcgacc gccggtcggc ggggcgctct tcggggtctg tgcgtgagcc atggctatac
     1321 ctcggggtcg ttcgcgggga cgttgatctc gactcgggcc ccgtcgggat caaaaaagaa
     1381 gaactggcgc ggaccaccgc cgggaacatc gttgcgaacc ggctgtatgt cgcagcgctc
     1441 caggcgttgg cagacagcgt catagtcgtg ggcgacgaac gcgatgtgat cgaccggccc
     1501 gggcccgccc gcccgctcgg ggaccgccag acccaacgtc tgtgcgtggg cgcgatatga
     1561 gccacggtgg gcgatgtgca ggcacggccg ttcgctcgcg tagagccaga agccgtcgaa
     1621 ctccaagggc ggacgaggcc cggcgtggag gccgagggca ccttcgtaga agtccctgga
     1681 gcgctcgatg tcgtcgctga gcacgagcac gtggtcgagt gcgttgacgc ccggcgtggt
     1741 gctcatccgg ttgtggggag gctttcgttg ggggtggtgg tgtcgccgcg taggaggtag
     1801 gcggagtgca ggagttcggg gggggttttg agttggtggg cggtgccgga gtattggatg
     1861 tggccgcctt cgatgacgta ggcgcggttg gcgaggtcga gcgcgacggc ggcgaattgt
     1921 tcgatcaaga ggacgccgat gccgttgtgg gtgacggtgt cgatggtcgg gatgaggcgt
     1981 tgcacgacga cgggcgcgag gccgagggag agttcgtcga tgatgatgaa cttgggttgg
     2041 gagaccagtg cttgggcgag gacgaccatt tgttgttcgc cgccggagag ggcgcgggct
     2101 tggttgtgga ggcgtttttc gagttcgggg aacagttcga gcgcgtgtgt gcgggcggcg
     2161 gcgccggctt tggccgagag ggtgtaggtc gcgattttga ggttgtcttc gacggttagt
     2221 tccccgagga ggcggcggcc ttcggggacg atcgccaggc cggcgcggcg gatgcgttcg
     2281 gggcgtttgc cggcgagttg ttcaccggcg agcgtgaccg tcccggctgt gagtggcagg
     2341 acgccgccga cggcgaggac catgctggat ttccccgcgc cgttggggcc caggagggcg
     2401 gtgacttcgc cgggggcgac gtcgatggtg atgtcgtgca cgacatcgcg tcccccgcgc
     2461 gcgacggtca ggttcgcgag acgcagaccc ccttccacga tcggactgtc ggggctggcg
     2521 ggagcgctcg gggtctcggg ggtgctcatg cggcgatgtt ctcggtgccg aggtaggcct
     2581 tgacgacgtt ggggtcgcgg aggacctggt cggtcgggcc cgacgcgatc agcttgccga
     2641 agtcaaggac ggcggtgttc tgacagcacg acgagacgag actcatgtcg tgatcgacga
     2701 ggatcgtcaa ggcgccggtg cgttcgggga tcgctgtgat gatctgtccc atccgcgtgg
     2761 tctcctcatc ggggaggccg gcggcgggtt cgtctaagag gacgaggcgg ggtttgccga
     2821 cgatcgcgcg ggcgagctcg
//
LOCUS       DATCCH010000290         2230 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_489273, whole genome shotgun sequence.
ACCESSION   DATCCH010000290 DATCCH010000000
VERSION     DATCCH010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489273"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /gene="carA"
                     /locus_tag="VIJ44_05590"
     CDS             <1..784
                     /gene="carA"
                     /locus_tag="VIJ44_05590"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEY5165404.1"
                     /translation="GFGDYLHRHGVPAIAGIDTRRLTRHLRDAGAMPGAFGADESAAT
                     GIARDAPGTEGIDLVAEVTTADAYTVGDDDAPFRVVAYDYGIKTTLLRHLVGAGCHVE
                     VVPATTPAADVLARHADGVFLSNGPGDPEAVTGAVETVRSLLGAVPVFGICLGHQILG
                     LALGATKHKLPFGHHGANHPVRHESTGHVEITSQNHNYAVDVDALPDVHRTHVNLNDG
                     ICEGLSALDVEAFSVQYHPEAGPGPHDATYLFADFTALLAGT"
     gene            786..>2230
                     /gene="carB"
                     /locus_tag="VIJ44_05595"
     CDS             786..>2230
                     /gene="carB"
                     /locus_tag="VIJ44_05595"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEY5165405.1"
                     /translation="MPRRDDLHSILLLGSGPIVIGQACEFDYSGTQACRVLRREGYRV
                     VLVNSNPATIMTDPEFADATYIEPLDVETVEQVIERERPDALLPTLGGQTALNLAIGL
                     HDAGVLERFGVELIGANVDAIHTAEDRSRFKAAMLEIGLSVPRSGLAYSVEEAVTVAA
                     DVGYPVVIRPSFILGGGGTGFATDDETMRAIAEHGLAVSPVGEILVERSVRGWKEFEL
                     EVMRDHADNAVVVCSIENLDPMGVHTGDSITVAPAQTLTDVEYQRMRDAAFACIRRIG
                     VDTGGSNIQFAVDPATGEMVVIEMNPRVSRSSALASKATGFPIAKIAACLAVGYRLDE
                     IQNDITGVTPASFEPTIDYVVTKVPRWAFEKLPGASPVLGTQMQSVGEVMAIGRTFPE
                     SLQKALRSLETGRDGLNADPNEEIADRLDTDELVRRTAVATPDRVFLLEAALRRGVTV
                     ERLHEVTDIDPWFLDQIARIADVRTELAGHTP"
BASE COUNT          357 a          873 c          682 g          318 t
ORIGIN      
        1 agggttcggc gactatctcc accgtcacgg ggtccctgcg atcgccggca tcgacacccg
       61 acgcctcacc cgccatctcc gcgacgccgg agccatgccg ggagcgttcg gcgccgacga
      121 gagcgccgcc accggcattg cccgcgacgc gcccggcacc gaaggaatcg acctcgtcgc
      181 cgaggtcacc accgctgacg cgtacaccgt cggcgacgac gacgcaccgt tccgggtcgt
      241 cgcctacgac tacgggatca agacgaccct gctgcgccac ctcgtcggcg cgggctgtca
      301 cgtcgaggtc gtcccggcca ccacccccgc cgccgacgtc ctggcccgcc acgccgacgg
      361 ggtgttcctc tcgaacggcc ccggcgaccc cgaggcggtc accggcgcgg tggagaccgt
      421 ccgatcgctg ctcggtgcgg tcccggtgtt cggcatctgc ctcggccacc agatcctcgg
      481 tctcgcgctc ggcgccacca agcacaagct cccgttcggg catcacggcg ccaaccaccc
      541 ggtgcgccac gagtcgacgg gccacgtcga gatcaccagc cagaaccaca actacgccgt
      601 cgacgtcgac gcgctccccg acgtgcaccg cacccacgtg aacctcaacg acgggatctg
      661 cgaagggctc tccgcgctcg acgtggaggc cttctcggtt cagtaccacc ccgaggccgg
      721 accgggcccc cacgacgcca cctatctctt cgccgacttc accgcgctgc tcgccgggac
      781 ctgagatgcc ccgccgcgac gacctgcaca gcatcctgct gctcggcagc ggacccatcg
      841 tgatcggtca ggcctgcgag ttcgactact cgggcacgca ggcctgccga gtccttcgcc
      901 gtgagggcta ccgagtggtg ctggtgaact cgaacccggc gacgatcatg accgaccccg
      961 agttcgcgga cgccacctac atcgagccgc tcgacgtcga gaccgtcgag caggtcatcg
     1021 agcgcgagcg acccgacgcg ctcctcccca ccctcggcgg gcagaccgcg ttgaacctgg
     1081 ctatcggact gcacgacgcc ggggtcctcg aacggttcgg tgtcgagctg atcggcgcca
     1141 acgtcgacgc catccacacc gccgaggacc gttcccgctt caaagctgcg atgctcgaga
     1201 tcggcctcag cgtgccccgc tccggtctcg cctactcggt cgaggaggcc gtcacggtcg
     1261 ccgccgacgt cggctacccg gtcgtcatcc gcccctcctt catcctcggt ggcggcggca
     1321 ccggcttcgc caccgacgac gaaacgatgc gcgccatcgc ggagcacggc ctcgcggtca
     1381 gccccgtcgg ggagatcctg gtcgaacggt cggtgcgagg ctggaaggag ttcgagctcg
     1441 aggtgatgcg cgaccacgcc gacaacgcgg tcgtcgtctg ctccatcgag aacctcgatc
     1501 cgatgggggt ccacaccggc gactcgatca ccgtcgctcc cgctcagacc ctcaccgacg
     1561 tcgagtacca gcgaatgcgt gacgccgcgt tcgcctgcat ccgccggatc ggcgtcgaca
     1621 ccggcggctc gaacatccag ttcgcggtcg atcccgccac cggcgagatg gtcgtcatcg
     1681 agatgaaccc ccgcgtgtca cgctcgagcg cgttggcgag caaggccacc ggcttcccga
     1741 ttgccaagat cgccgcctgc ctcgccgtcg ggtaccgcct cgacgagatc caaaacgaca
     1801 tcaccggcgt gacgcccgcc tctttcgagc cgaccatcga ctacgtcgtc accaaggtgc
     1861 cgcgctgggc gttcgagaag ctccctggcg cgtcccccgt gctcggaacc cagatgcagt
     1921 cggtcgggga ggtcatggcc atcggccgca ccttccccga atcgctccag aaggcgctcc
     1981 gcagcctcga gaccggacgg gacggcctca acgccgaccc caacgaggag atcgccgacc
     2041 ggctcgacac cgacgagctg gtgcgccgga ccgcggtcgc gactcccgac cgggtgttcc
     2101 tcctcgaagc ggcgctccgc cgcggcgtca ccgtcgaacg cctccacgag gtcaccgaca
     2161 tcgacccctg gttcctcgac cagatcgccc ggatcgccga cgtccgcaca gagctggcgg
     2221 gccacactcc
//
LOCUS       DATCCH010000291         2342 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_115262, whole genome shotgun sequence.
ACCESSION   DATCCH010000291 DATCCH010000000
VERSION     DATCCH010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115262"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1208)
                     /locus_tag="VIJ44_05600"
     CDS             complement(<1..1208)
                     /locus_tag="VIJ44_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase large
                     subunit"
                     /protein_id="HEY5165406.1"
                     /translation="MSEVIGRFRFMDAQRVNLDGFAVEDASIGLIAFASPADPEPSLV
                     IGADGRVLEMDSRAARDFDILDEFIARHGIDLLVAPEAMATTDLAYARMCVDPAVPRA
                     ELVRLASGMTPAKLACCLALLRPVELGMAMTKLRARRTPSNQAHVTNRSDDPLLLAAD
                     AATAAAFGFREVETTVPVLGDAPSNAVAVSIGAAVGSPGVLVQCSVEEALELEMGMRG
                     LVSYAETVSLYGTEQVFVDGDDTPWSKAFLTSAYASRGVKMRVTSGGGAEALMGAAQG
                     CSMFYLEARCVSLARAIGAQGVQNGGIDSAAVAGSVPGGVRSLMAENVMVMARNLEAC
                     TGNDALMSESDVRRTSRTLPILLAGSDYVCSGFGSIQRYDNMFGPSQWNAEDVDDFLV
                     MQRDWGVDGGL"
     gene            complement(1205..1633)
                     /locus_tag="VIJ44_05605"
     CDS             complement(1205..1633)
                     /locus_tag="VIJ44_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012892649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEY5165407.1"
                     /translation="MTRTEIDLALARAMVASALVEATASGALVSAAVVDAGGHLIAFE
                     RMDGAEIAGPVLAPDKAFTAVAHRIATHELAPLVAPGGPLVGMNAAAGGRYVCFAGGL
                     PLWSDDRVVGGIGVSGGTAEQDLAASSAALAVYEGREEVG"
     gene            complement(1630..>2342)
                     /locus_tag="VIJ44_05610"
     CDS             complement(1630..>2342)
                     /locus_tag="VIJ44_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016433826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEY5165408.1"
                     /translation="SLTGTGFTSLLQPALPERTWFYEIDRASRVVRYAALDTRYTAEL
                     ALDPMHGTVGVAPGLGEVRSSLAPGDWGGNMDTPEMRAGVTCYLGVNVPGALLSLGDG
                     HARQGEGETCGVAVECAMDTVFVVDLIKGVPTPWPRLESDDHIMTTGSTRPLEDAFRI
                     AQVQMVRWVAELTGLGEMDAYQLVSQTTLSPVANVVDTVYTMVAKVPKSVLARPTVMQ
                     GMHDRMRALSSGAATWAG"
BASE COUNT          357 a          876 c          791 g          318 t
ORIGIN      
        1 agacctccgt cgactcccca gtcgcgctgc atgacgagga agtcgtcgac gtcctccgcg
       61 ttccactggc tcgggccgaa catgttgtcg tacctctgga tggacccgaa gccgctgcac
      121 acgtagtccg acccggccag caggatcggg agggtacgag aggtgcgccg tacgtcggac
      181 tccgacatca gcgcgtcgtt gcccgtacag gcctcgaggt tcctggccat caccatcacg
      241 ttctcggcca tcagcgaccg gaccccgccg ggtaccgagc cggcgaccgc tgccgagtcg
      301 atgccgccgt tctgcacgcc ctgtgcgccg atcgcgcggg ccagggacac gcaccgggcc
      361 tccaggtaga acatcgagca gccctgggcg gcacccatga gcgcctcggc cccgcccccg
      421 gaggtcaccc gcatcttcac accgcgcgag gcgtacgcag atgtcaggaa cgccttggac
      481 cacggtgtgt cgtcaccgtc gacgaagacc tgctcggtcc cgtacagcga caccgtctcg
      541 gcgtacgaga ccagtccgcg catccccatc tcgagctcca gcgcctcctc caccgagcac
      601 tggacgagca ccccgggcga gccgaccgcc gcgccgatcg agacggcgac cgcgttggac
      661 ggggcgtcgc cgagcaccgg cacggtcgtc tcgacctcgc ggaagccgaa ggccgccgcg
      721 gtcgcagcgt cggcggcgag cagcaacggg tcgtcggaac ggttggtgac gtgggcctgg
      781 ttgctggggg tacggcgagc ccgcagcttg gtcatggcca tgccgagctc gaccggccgc
      841 aggagtgcga ggcagcaggc cagcttggcc ggcgtcatcc ccgatgcgag ccgcacgagc
      901 tccgcgcgcg gcacggccgg gtccacgcac atgcgggcgt atgcgagatc ggtcgtggcc
      961 atggcttcgg gagccaccag caggtcgatc ccgtgccggg cgatgaactc gtcgaggatg
     1021 tcgaagtcgc gggcggcgcg ggagtccatc tcgaggaccc ggccgtccgc accgatcacc
     1081 aggctgggct ccgggtcggc gggcgacgcg aaggcgatca gcccgatcga cgcgtcctcg
     1141 acggcgaagc cgtccaggtt cacccgctgc gcgtccatga agcggaagcg cccgatcacc
     1201 tcgctcatcc gacctcctcg cgcccctcgt agacggcgag cgcggccgag gacgcagcca
     1261 ggtcctgctc cgccgtaccg ccgctcacac cgatgccgcc gaccacgcga tcgtcggacc
     1321 acagcggcag cccgccggcg aagcacacgt agcgcccgcc ggccgccgcg ttcatgccta
     1381 cgagcggccc gccgggtgcg accagcggcg cgagctcgtg cgtggcgatc cggtgcgcga
     1441 cggccgtgaa ggccttgtcc ggggccagga ccggcccggc gatctcggca ccgtccatcc
     1501 gctcgaacgc gatcaggtgc ccaccggcgt cgaccaccgc ggccgacacc agggcgcccg
     1561 acgccgtggc ctcgacgagc gctgacgcca ccatcgcacg ggcaagcgcg aggtcgatct
     1621 cggtgcgcgt caccctgccc atgtcgccgc cccgctcgag agggcccgca tccgatcgtg
     1681 catcccctgc atcaccgtcg gacgcgcgag caccgacttc ggcaccttgg ccaccatcgt
     1741 gtagacggtg tccacgacgt tggccaccgg cgacagcgtc gtctgcgaca ccagctggta
     1801 ggcgtccatc tcgccgagcc cggtgagctc tgccacccat cgcaccatct gcacctgggc
     1861 gatccggaac gcgtcctcga ggggtcgggt ggaaccggtc gtcatgatgt ggtcgtcgct
     1921 ctccagccgc ggccaggggg tcggtacgcc tttgatcagg tcgaccacga acaccgtgtc
     1981 catcgcgcac tcgacggcga cgccgcacgt ctcgccctcg ccttggcgcg cgtgcccgtc
     2041 gccaaggctg agcagcgcgc ccgggacgtt gacgcccagg tagcacgtca cccctgcgcg
     2101 catctccggg gtgtccatgt tgccgcccca gtcgccgggc gccagtgagg agcggacctc
     2161 gcccagcccc ggcgccacac ccacggtccc gtgcatcggg tccagcgcaa gctccgcggt
     2221 gtaccgcgtg tcgagggcgg cgtagcgcac cacccggctg gctcggtcga tctcgtagaa
     2281 ccaggtacgt tccggaagcg ccggctggag cagcgaggtg aagccggtac cggtgaggga
     2341 gc
//
LOCUS       DATCCH010000292         2760 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_389908, whole genome shotgun sequence.
ACCESSION   DATCCH010000292 DATCCH010000000
VERSION     DATCCH010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389908"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1237)
                     /locus_tag="VIJ44_05615"
     CDS             complement(<1..1237)
                     /locus_tag="VIJ44_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003950369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEY5165409.1"
                     /translation="MKAIAKTSAAETGNQAMGACPVLTSHRAGWFPPGGTLEVVSVAS
                     SSPDPSTRRPDIADSLLDLVGNTPLVRLGRIGRDLACDLVAKVELFNPGGSVKDRPAI
                     AMIDDAERRGLLGPGGTIVEPTSGNTGVGLAIVAAQRGYRCIFVMSDKMSDEKVALLR
                     AYGAEVVVCPTAVPPEHPDSYYSVADRLTRETPNAFRPNQYYNPANPDEHERSTGPEI
                     WRQTSGRITHLVAGVGTGGTITGVARYLKGQNPEVQVIAADPEGSVFSGGTGRPYLVE
                     GVGEDFWPPTYDPSVVDRTIMVSDTQSFAAARRVTREEGLLIGGSCGTAVHAALVVGA
                     ELGPEALVVVILPDSGRGYLSKIFNDDWMTKFGFLVRRGPTAGDVLGAKNASSDRSIP
                     DLVLITPDEPARRGFALMRD"
     gene            complement(1493..1648)
                     /locus_tag="VIJ44_05620"
     CDS             complement(1493..1648)
                     /locus_tag="VIJ44_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165410.1"
                     /translation="MHEPSHGALGLSLPAGQAAVARRAEPGANRDHASLMIGVDLDRR
                     HAAIREV"
     gene            1793..2299
                     /locus_tag="VIJ44_05625"
     CDS             1793..2299
                     /locus_tag="VIJ44_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide cyclase / dehydrase and lipid
                     transport family protein"
                     /protein_id="HEY5165411.1"
                     /translation="MQEHSFTVDLDAPRDEVWEMFWYRAPDRPQPRDVRTRIEILHPG
                     DDIGEGLVRHCWFPVPRFLLSGGVGQSWEWLTEVRPIESWRYDAIGKPLWSRATGHTR
                     LEDLGEARTRVHFHETYETFNPLMRRVGLERYVHRRLSRDNDTILAALEGGLRWHRAR
                     HRGADQST"
     gene            complement(2287..>2760)
                     /locus_tag="VIJ44_05630"
     CDS             complement(2287..>2760)
                     /locus_tag="VIJ44_05630"
                     /inference="COORDINATES: protein motif:HMM:NF014801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEY5165412.1"
                     /translation="GSAGVALLPGFERERASRQPDVGGVLTGLRAHVGPEHLARAAVE
                     GVVCSVLDGLDALRAADVPVGGRISLIGSGARSHAFQRILADLSGRAVTVPQGERVAT
                     GACVQAAAALHGQPPEQIAAAWALAPTRQLDPSDRGDSAAVRDRYRAAQARSYVD"
BASE COUNT          436 a         1007 c          926 g          391 t
ORIGIN      
        1 ggtcgcgcat gagcgcgaag ccgcgccgag cgggctcgtc gggggtgatg agcaccaggt
       61 cggggatcga acggtcgctg ctggcgttct tggcgccgag cacgtcaccg gcggtcggtc
      121 cacgccgaac gaggaacccg aacttcgtca tccagtcgtc gttgaagatc ttggacaaat
      181 agccgcgacc cgagtcggga aggatcacga cgacgagggc ttcggggccg agctccgcgc
      241 cgaccaccag cgcggcgtgg accgcggtgc cgcacgagcc accaatgagc agcccctcct
      301 cgcgggtgac gcgtcgcgcc gcggcgaacg actgggtgtc ggacaccatg atcgtgcggt
      361 ccaccacgct cgggtcgtac gtcggcggcc agaagtcctc accgacgcct tcgacgagat
      421 acggacgtcc cgtcccaccc gaaaacaccg agccttcggg atccgccgcg atgacctgca
      481 cctcagggtt ctggcccttc aggtaccgag ccacgccagt aatggttcca ccggtcccga
      541 cccccgcgac cagatgggtg atacgacccg aggtctggcg ccagatctcg ggtccagtgg
      601 agcgctcgtg ctcatcgggg ttcgccgggt tgtagtactg gttcggtcgg aacgcgttcg
      661 gggtctcgcg ggtcaggcgg tccgccaccg agtagtacga gtcgggatgc tcaggtggga
      721 cggcggtcgg gcacaccacg acctcagctc cgtacgcgcg gagcagcgcc accttctcat
      781 cggacatctt gtccgacatc acgaagatgc agcgataacc ccgctgcgcc gcgacgatgg
      841 ccaacccgac accagtgttc ccggaggtgg gctcgacgat cgttcctccg ggcccgagca
      901 gcccccgccg ttcggcatcg tcgatcatgg caatcgccgg gcggtccttg acgctcccgc
      961 cggggttgaa gagctcgacc ttcgcgacga gatcgcacgc caggtctcgc ccgatgcggc
     1021 cgaggcgcac cagcggcgtg ttgcccacga ggtcgagcag cgaatccgcg atgtcgggcc
     1081 gacgcgtcga ggggtcgggc gaagacgaag cgaccgacac gacctcgagc gtaccgccgg
     1141 gcgggaacca ccccgcccga tgcgacgtca aaacgggaca agcacccatc gcctggttgc
     1201 ccgtctccgc tgcgctcgtc ttcgcgatcg ccttcacgac cggccatcct ctggtgcgga
     1261 tcccacatcc ctctggtgcg tccgaggtcg tgctgcacgt gacgacgttc ggtgtcgtcg
     1321 acccctccct gggccgcgtc accgttcgcg gcgacggcaa tcatccagcc ggcctggcgc
     1381 gaggtcacgc gtcgccggag ccgctgtcct ccgggccggc aagaacggtg tgcagcgcac
     1441 gctgcgggcg gcgcgtgccg ccgggttact cgatggcgac gacttcggct cgtcacacct
     1501 cacggatcgc ggcgtgacga cgatcgagat ccacgccaat catcagcgac gcatgatctc
     1561 ggttcgcgcc gggctcggcc cggcgggcga ccgcggcctg accagccggg agcgacaggc
     1621 ccaacgcgcc gtgcgacggt tcgtgcacct cgccctcgaa ccgcgttcgc tcgccgagcc
     1681 caccaaagcg acgcccgcgc ctgacctacg ctccgctcga cctcgggcct cgggtcgtgg
     1741 cggtgtcgcg gccgcgcgga cacccgggcc gagacggcgg aaggcgacac ggatgcagga
     1801 acactcgttc acggtcgacc tcgacgcgcc ccgcgacgag gtctgggaga tgttctggta
     1861 tcgggctccg gaccgacccc agcctcgcga cgtccgcact cgaatcgaga tcctgcatcc
     1921 cggcgacgac atcggcgagg gactcgtgcg ccactgctgg ttcccggtcc cccggttctt
     1981 gctgtccgga ggcgtgggcc agtcctggga atggctcacc gaggtccgtc ccatcgagtc
     2041 atggcgctac gacgcgatcg gcaagccgtt gtggtcgcgg gcgacgggcc atacccggct
     2101 cgaggacctc ggcgaggcac gaacccgggt ccatttccac gagacgtacg agacgttcaa
     2161 cccgttgatg cgccgcgtgg gcctggaacg ctacgtgcat cgccgcctgt cccgcgacaa
     2221 cgacaccatc ctcgccgcgc tcgaaggcgg cctgcgctgg caccgcgccc gccaccgcgg
     2281 cgcggatcag tccacgtaag agcgcgcctg cgcggcacgg taccgatcac ggaccgcggc
     2341 gctgtcacca cggtcgctcg ggtcgagctg gcgcgtcggc gccagcgccc acgccgccgc
     2401 gatctgctcc ggcggctggc cgtgcaacgc cgccgcggcc tgcacgcacg ccccggtcgc
     2461 gacccgctca ccttggggaa ccgtcaccgc ccgtccggaa aggtcggcca ggatccgctg
     2521 gaacgcatgc gatcgagcgc cggacccgat cagcgaaatc cgtccaccga ccggcacgtc
     2581 agcggctcga agcgcgtcga ggccatcgag caccgagcag acaacccctt cgaccgcggc
     2641 gcgagcgagg tgctcgggcc cgacgtgagc gcgcagtccc gtgagaaccc ctccgacatc
     2701 gggctggcgg gacgcccgtt cgcgctcgaa ccctgggagg agcgccaccc cggccgatcc
//
LOCUS       DATCCH010000293         2096 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_140350, whole genome shotgun sequence.
ACCESSION   DATCCH010000293 DATCCH010000000
VERSION     DATCCH010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140350"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="VIJ44_05635"
     CDS             <1..359
                     /locus_tag="VIJ44_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165413.1"
                     /translation="KDLTLAYQTLIRPRSRAAYDRRHPHQPATTAPVNPVDPVSPLRR
                     DPIFRTEGRARAAVVSGVVLFVLGVACAVLLSVVDTGGTGETVTLWIAAAKLLVAGPV
                     LVGAGLLRLQRIATGQ"
     gene            396..1250
                     /locus_tag="VIJ44_05640"
     CDS             396..1250
                     /locus_tag="VIJ44_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HEY5165414.1"
                     /translation="MEVKRVGVVGSGIMGAGVAEVAAKAGFEVVVRSRAQGSADALVA
                     GLEKSLGKQVEKGRLDDAERQAILARLSVTTDLGVLADCDLVVESVVEDLATKRHLFN
                     ELDRVCADSAVLATNTSTLPVVDLAMETGRPDQVCGIHFFNPAPMMALVEVVRAITSS
                     DETVATARAFAERCGKTVVDVRDQAGFIVNALLFPYLNNAVKLLDAGVASRDDIDAAM
                     KGGCNFPMGPLELLDLVGLDTSLAILEALYAEFNDPNYAPAPLLRRMVSAERYGRKSR
                     KGFYDYER"
     gene            1324..>2096
                     /locus_tag="VIJ44_05645"
     CDS             1324..>2096
                     /locus_tag="VIJ44_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEY5165415.1"
                     /translation="MRTYSGHSSATASNELYRTNLAKGQTGLSIAFDLPTQTGYDPDD
                     PLARGEVGKVGVPVPHLGEMRTLFDGIPIEEMNTSMTINATAMWLLGMYLALAEERGI
                     DPGRLAGTTQNDIVKEYLSRGTYIFGPEASRRLTVDLICHTVRHVPRWNPINVCSYHL
                     QEAGATPVQEIAYAFATAFGVLDAVRESGQVKPEDLPVVVGRMSFFVNSGIRFVEEMC
                     KVRAMGRLWDRLTAERYGVTDPKLRRFRYGVQVNSLGLT"
BASE COUNT          326 a          738 c          703 g          329 t
ORIGIN      
        1 tcaaggacct caccctcgcc taccagaccc tcatccgccc ccgcagccgc gccgcctacg
       61 accgccggca tccccaccag cccgccacca ccgcgccggt caacccggtc gatccggtca
      121 gtccgcttcg ccgtgacccg atctttcgca ccgaaggacg ggcccgggcc gccgtcgtgt
      181 ccggtgtcgt cctcttcgtc ttgggggtgg cgtgcgccgt gctcctcagc gtcgtcgata
      241 ccggtggcac cggcgagacc gtcaccttgt ggatcgccgc cgccaagctg ctggtcgcgg
      301 gcccggtcct ggtcggcgcc ggtctccttc ggctgcaacg gattgctact gggcagtaac
      361 ctgatggctt ccgtctcggc cgagaggaga caccggtgga ggtcaaacgg gtcggagtcg
      421 tcggctccgg gatcatgggc gccggcgtcg ccgaggtggc cgcgaaggcc gggttcgagg
      481 tcgtggtccg cagccgagcc cagggcagcg ccgacgcgct cgtcgccggg ctcgagaagt
      541 cgctcggcaa gcaggtcgaa aagggccggc tcgacgacgc cgaacgccag gcgatcctgg
      601 cgcggctgtc ggtgacgacc gacctcggag tgctcgccga ctgcgaccta gtcgtcgaat
      661 ccgtcgtcga ggatctggcg acgaaacggc atctgttcaa cgagctggac cgggtctgtg
      721 ccgattcggc ggtgctcgcc accaacacct ccacgctgcc ggtcgtcgac ctggcgatgg
      781 agaccgggcg gcccgatcag gtctgtggca tccacttctt caacccggcg ccgatgatgg
      841 ccctcgtcga ggtggttcgg gccatcacca gctccgacga gacggttgcg acggcccggg
      901 cgttcgcgga gcggtgcggc aaaaccgtcg tcgacgtgcg ggaccaggcc gggttcatcg
      961 tcaacgccct tctgttcccc tatctgaaca acgcggtcaa gctgctcgac gccggcgtcg
     1021 ccagccgaga cgacatcgac gccgcgatga agggggggtg caacttcccg atgggccccc
     1081 tcgagctgct ggacctggtc ggactcgaca ccagcctggc gatcctcgag gcgctctacg
     1141 ccgagttcaa cgaccccaac tacgcgccgg cgccgctgct gcgccggatg gtgagcgccg
     1201 agcggtacgg gcgcaagtcc cggaagggct tctacgacta cgagcgctga gccgccacgt
     1261 tcggggtcgt caccagcgcc ggaggcgacg agaatggacc cggtgaagga ccacccgtgg
     1321 ttgatgcgga cctactcggg tcactcgtca gccaccgcgt cgaacgagct gtaccgcacc
     1381 aacctggcca agggccagac cgggctgtcg atcgccttcg acctccccac ccagaccggg
     1441 tacgaccccg acgaccccct ggcgcggggc gaggtcggca aggtcggtgt ccccgtccct
     1501 cacctcggtg agatgcggac cctcttcgac gggatcccca tcgaggagat gaacacctcg
     1561 atgaccatca acgccaccgc gatgtggctg ttggggatgt acctggcgct cgccgaggag
     1621 cggggcatcg acccgggccg actcgccggg acgacccaga acgacatcgt caaggagtac
     1681 ctcagtcggg gcacctacat cttcgggccc gaggcgtccc ggcgtctcac cgtcgacctg
     1741 atctgtcaca ccgtgcggca cgtcccgcgg tggaacccga tcaacgtctg ctcgtatcac
     1801 ctccaggagg ccggcgcgac accggtccag gagatcgcgt acgcgttcgc caccgcgttc
     1861 ggggtcctcg acgcggtgcg cgagtccggc caggtcaagc cggaggacct gccggtcgtg
     1921 gtcgggcgga tgtcgttctt cgtcaactcg ggcatccgct tcgtcgagga gatgtgcaag
     1981 gtgcgggcga tgggccgact ctgggatcgc ctcaccgcgg agcggtatgg cgtcaccgac
     2041 ccgaagctgc ggcgcttccg gtacggggtc caggtcaact cgctcgggct caccga
//
LOCUS       DATCCH010000294         2836 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_15720, whole genome shotgun sequence.
ACCESSION   DATCCH010000294 DATCCH010000000
VERSION     DATCCH010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15720"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="VIJ44_05650"
     CDS             <1..742
                     /locus_tag="VIJ44_05650"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEY5165416.1"
                     /translation="APAIAPDTPIISLSKGVEQDTHLRMTQVIAEVLTGHRADRIGVL
                     TGPNLAREVAEGQPTASVVAMVDPDATASLQRLFMTTTFRVYTNPDVVGCEIAGALKN
                     VVAIAAGIAAGLGYGDNTKAALITRGLAELARLGVALGGEPLTFSGLAGMGDLVATCA
                     STKSRNRTVGVELGRGRSLADILSEMNMVAEGVKSTGAVLALAAQHDVDMPIAAMVGA
                     VLYDGRRPADLVPTLMLREAKPELDGIR"
     gene            739..1917
                     /locus_tag="VIJ44_05655"
     CDS             739..1917
                     /locus_tag="VIJ44_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165417.1"
                     /translation="MTTAVAAIGSRRQVRVDDRGRVEPFDATWSIDWWVGADDRWHRP
                     AHEPTLRQTLVDAVPVVRTAIRVPGGDAVQHVYAAGGPDLVVIDVENESPAPFVLALV
                     VRGAERLTLDDGTLTIDGDAGVLVTTRAPSRWAASVDGTTEETVTSGAASDAPFVPLR
                     ARGGQLEAAFLYPVAHRSAIRFALHLGPGHLEERAVARLPEPGAAARGWAAHLDRGLR
                     VELPDPSLTAALRSALAATLLAGARRPVAPGAVAALEDWGFDTEATSAWTTLGWRDRR
                     AAAHRPAMPPSWADVAATRGDAELLTHVRALLAHDGADGIVTLLADLPRSWRGHRVDV
                     RDAPTRAGPVSYAIRWHGPRPALLWEAPPGVQLRAPGLDVGWTTRDPSGEALLAADVA
                     "
     gene            1917..>2836
                     /locus_tag="VIJ44_05660"
     CDS             1917..>2836
                     /locus_tag="VIJ44_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165418.1"
                     /translation="MDPEELEALGLYDPAGPDADERRALLALALEHGATIDEIHAAID
                     GARLHALAVERLLVPHTPNLTLGAVANAAKVTPEFATRVWRALGFALAADDAVVCTET
                     DVATLVFYGLLASQVSDTAAVSLARATGSSLSRVADAHVAMVRSGIEAPLRDAGGTDV
                     DVARGFVDMASVMVPSIYPMFEAVHRRHLVEAARRYSLWGTRATEASTTGAVVGFADL
                     VGYSALNQHLSVTELDDLVSSFEQRVLDAVARPGARLVKVIGDEAMFATARATDAAAV
                     AHELLDGSDVLPLRIGIASGTVLARAGDLFG"
BASE COUNT          379 a         1079 c          986 g          392 t
ORIGIN      
        1 ggctccggcc atcgcaccgg acacgccgat catcagcctg agcaaggggg tcgagcagga
       61 cacgcacctg cgcatgaccc aggtcatcgc cgaggtcctg accgggcacc gcgccgaccg
      121 catcggggtc ctcaccggac cgaacctggc ccgcgaggtc gccgaaggcc aaccgaccgc
      181 gtccgtggtc gccatggtcg acccggatgc caccgcgtca ctccaacggc tgttcatgac
      241 gacgacgttc cgggtgtaca ccaaccccga cgtcgtcggc tgcgagatcg ccggcgcgtt
      301 gaagaacgtc gtcgccatcg cggccgggat cgccgcgggg ctgggatacg gcgacaacac
      361 caaagccgcg ctcatcaccc gcggcctcgc cgagctggcc cgcctcggcg tcgcgctcgg
      421 cggtgagccc ctcacgttct cgggcctcgc cggcatggga gacctggtcg cgacctgcgc
      481 cagcaccaag agccgcaacc gcaccgttgg cgtcgagctc ggccgggggc gctccctcgc
      541 tgacatcctg tccgagatga acatggtggc cgaaggggtc aagagcacgg gcgcggtcct
      601 cgctctcgcg gctcaacacg acgtcgacat gccgatcgcg gcgatggtcg gtgcggtcct
      661 gtacgacggt cgacgtcccg ccgacctcgt accgacgctc atgttgcgcg aggcgaagcc
      721 cgagctcgac ggcatccggt gaccaccgcg gtcgctgcga tcggatctcg ccgacaagtg
      781 cgcgtcgacg accgcggccg agtcgaaccg ttcgacgcca cctggtcgat cgactggtgg
      841 gtcggagccg acgaccgctg gcaccgaccc gcccacgagc ccacgctccg ccagaccctg
      901 gtcgacgccg tcccggtggt ccgcaccgcg atccgcgttc cgggtggaga cgcggtgcag
      961 cacgtctacg ccgccggagg gccggacctc gtcgtgatcg acgtggagaa cgagtctccg
     1021 gccccgttcg tgctggcgct ggtcgtgcga ggcgccgaac gcctgaccct cgacgacggg
     1081 acgttgacca tcgacggtga cgccggcgtg ctcgtcacca cgagagcacc gtcgcgttgg
     1141 gcggccagcg tcgacggcac caccgaggag acggtgacgt cgggcgccgc gagcgatgca
     1201 ccgttcgttc cgctccgggc ccgtggaggc caactcgaag cggcgttcct ctatcccgtc
     1261 gcgcatcgca gcgcgatccg gttcgcgctg cacctcggtc ccggccacct cgaggagcgc
     1321 gcggtcgccc gcctcccgga gccgggcgcc gcggcccgag gttgggccgc tcacctcgac
     1381 cggggcctgc gcgtcgagct ccccgaccct tctttgaccg cggcgctgcg gtcggcgttg
     1441 gccgcgacgc tcctcgccgg tgcccgccgt ccggtggccc ccggcgccgt cgcggcactc
     1501 gaggactggg gtttcgatac cgaggccacc tcggcgtgga ccaccctggg gtggcgcgac
     1561 cggcgggcgg cggcgcaccg cccggccatg cccccgagct gggccgacgt cgcggccacg
     1621 cgcggcgacg cggagctgct cactcacgtc cgggccctcc tcgcccacga cggcgccgac
     1681 gggatcgtga ccctgctggc cgacctgccc cgctcctggc gcgggcaccg cgtcgacgtc
     1741 cgggacgcac ccacccgtgc cggcccggtc tcgtacgcga tccgctggca tgggccccga
     1801 cccgcgctgc tctgggaggc accgcccggc gtgcagctcc gggcgccggg actcgacgtc
     1861 ggctggacca cgcgcgatcc gagcggcgaa gcgctcctcg cggcggacgt ggcgtgatgg
     1921 accccgagga gctcgaagcc ctcggtctct acgacccggc tgggcccgac gccgacgagc
     1981 gacgggcgct gctcgccctg gcgctcgagc acggcgccac gatcgacgag atccacgccg
     2041 ccatcgacgg cgcccggctg catgcgctgg cggtcgagcg gctgctggtg ccacacaccc
     2101 ccaacctgac cctgggtgcg gtggccaacg ccgcgaaggt gaccccggag ttcgcgaccc
     2161 gggtgtggcg agccttgggc ttcgcgctcg cggccgacga cgccgtggtc tgcaccgaaa
     2221 cggatgtggc caccctcgtc ttctacggcc tgctggccag ccaggtcagc gacaccgcgg
     2281 cggtgtcgct ggcgcgagca accggctcct ccctctctcg ggtcgcggac gctcacgtcg
     2341 ccatggtgcg gtcgggcatc gaggcgccac tgcgcgacgc cggcggcacc gatgtcgacg
     2401 tggcccgcgg cttcgtcgac atggcgtcgg tcatggtgcc gtcgatctac ccgatgttcg
     2461 aagctgtcca ccgccggcac ctcgtcgagg ccgcccgtcg ctactcgctg tggggtacga
     2521 gggccacgga ggcgagcacc accggcgccg tcgtcggctt cgccgacctg gtcgggtaca
     2581 gcgcactgaa ccagcacctc tccgtcaccg agctcgacga cctggtcagc agcttcgaac
     2641 agcgggtcct cgatgctgtg gctcggcccg gggcccgtct ggtcaaggtg atcggagacg
     2701 aggcgatgtt cgccaccgcc cgcgccaccg acgccgccgc ggtggcccac gagctcctcg
     2761 acgggtccga tgttcttccg ctgcgcatcg ggatcgccag cggaacggtc ctggcccgag
     2821 caggcgacct gttcgg
//
LOCUS       DATCCH010000295         2133 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_198678, whole genome shotgun sequence.
ACCESSION   DATCCH010000295 DATCCH010000000
VERSION     DATCCH010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198678"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1696
                     /locus_tag="VIJ44_05665"
     CDS             <1..1696
                     /locus_tag="VIJ44_05665"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEY5165419.1"
                     /translation="SFVERFRREAHSAASLNHPNIVGVYDWGQEHGTYFIVMEYVEGQ
                     SLRDVLRAERTLPAVQAAAIGAEIADALAFAHAHGVVHRDVKPGNVLMTPTGQVKVTD
                     FGIAANPTDAAQGLTQTGSVMGTATYFSPEQAQGFHVDGRTDVYALGVVLYEMVAGFP
                     PFQADSPVAVAMKHVREQPVPPSQFVPDLPPDLERVILAALAKDVNTRYQSADALRDD
                     LVRFERGRPVTAPIASLASEDAPTMAATGAAAPTQAGEMWEQPPKKRWGPIVATVIGL
                     GLLAALLVVLLVPQLRDQLLGSSSAAAAPKVEVPDVTSGNPTFTLAAQRLKTAGFKVA
                     RTDVEVASLDAERVVKQSPSAGELLAKGKIVTLQVVSNTVTLPNLVGQTFDSAQQQLL
                     TLKLVAQRSDMSTTAQPAGTVLAMSPLAGTKAARGTAITLTVAKAPQIAVPDVRGQDQ
                     TQASTVLQTAGFQVQIVPQANATVPSGKVINTIPPPGTMANKGDAVQMVVSTGPSTIT
                     VPNVVGQARDNAIAALTAAGLSVQASCGTNSAVLSQNPAGGSMVPPGSRVAIACLA"
     gene            1753..>2133
                     /locus_tag="VIJ44_05670"
     CDS             1753..>2133
                     /locus_tag="VIJ44_05670"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEY5165420.1"
                     /translation="MPWRATRDRWAVLVSEVMLHQTQASRVEAAWPTFLAEFPTPAAM
                     AAAGPGDVIRAWGRLGYPRRARRLWEAASRIASNGWPEDLTELPGVGRYTAGALRAQA
                     DDADVGAVDVNIRRVVERVVGRRLS"
BASE COUNT          343 a          785 c          705 g          300 t
ORIGIN      
        1 gagcttcgtc gagcggttcc gccgtgaagc gcacagtgcg gcgtcactca accatccgaa
       61 catcgtcggg gtctatgact ggggacaaga gcacggcacg tacttcatcg tcatggagta
      121 cgtcgaaggt cagtcgctgc gggacgtcct gcgcgccgag cggaccctcc cggcggtgca
      181 agccgccgcg atcggcgccg agatcgccga cgcgctcgcc ttcgctcatg cccacggggt
      241 cgtccaccga gacgtcaaac ccggcaatgt gctgatgacc ccgaccggcc aggtcaaggt
      301 caccgacttc ggcatcgccg ccaacccgac cgacgcggcg cagggtctca cccagaccgg
      361 gtcggtgatg ggcaccgcca cctacttctc cccggagcag gcccaagggt tccacgtcga
      421 cggacggacc gacgtgtacg cgctcggggt cgtgctctac gagatggtgg ccggcttccc
      481 gccgttccag gccgacagcc cggtcgcggt cgcgatgaaa cacgtccgcg agcagccggt
      541 cccgccgtcg cagttcgtcc ccgaccttcc gcccgacctc gaacgggtca tcctcgccgc
      601 gttagccaag gacgtgaaca cccggtatca gtcggccgac gccttgcgcg acgacctcgt
      661 ccgcttcgag cgcgggcgcc cggtcacggc ccccatcgcg tccctggcta gcgaggatgc
      721 ccccacgatg gctgccaccg gagccgcggc tcccacccag gcgggcgaga tgtgggagca
      781 gcctccgaag aagcgctggg gcccgatcgt cgccaccgtc atcgggctgg gtctcctcgc
      841 ggcgctcctg gtcgtgctgc tcgtcccgca gctgcgcgac cagttgctcg ggagcagttc
      901 ggccgcggca gcacccaagg tcgaggtccc cgatgtgacg agtggcaacc cgacgttcac
      961 actcgcggcg cagcggttga agacggcagg gttcaaggtg gcccgcaccg atgtcgaagt
     1021 cgccagcctc gacgctgagc gcgtcgtgaa acagagcccg agcgccggcg agcttctggc
     1081 gaagggcaag atcgtgacgc ttcaagtcgt gagcaacacg gtcacactcc ccaacttggt
     1141 cggccagacg ttcgactcgg cccagcagca actcctgacc ctgaagctgg tggcgcagcg
     1201 ctcggacatg tcgacgacag cgcagccggc gggcacggtc ctggccatga gcccgctcgc
     1261 ggggacgaag gcggcgcgtg gcaccgcgat caccctcacc gtcgcgaaag ccccgcaaat
     1321 cgcggtgccc gacgtgcgcg gccaggacca gacgcaagcg tcgaccgtgc tgcaaacggc
     1381 gggctttcag gtccagatcg tcccccaggc caacgccacc gtcccctcgg gcaaggtcat
     1441 caacaccatc cccccgcccg ggaccatggc caacaaaggc gacgccgtcc agatggtggt
     1501 ctccaccgga ccgtcgacga tcacggtccc gaacgtcgtc ggccaggcgc gagacaacgc
     1561 catcgcggcc ctgaccgcag ccgggctcag cgtgcaggcc tcctgtggga ccaacagcgc
     1621 ggtgctgtcg cagaacccgg ccgggggatc gatggtcccg cccggttcga gagtcgcgat
     1681 cgcctgcttg gcgtagtggc gggcccccgt tccccactca agacctggta cgtcgaccac
     1741 ggtcgccatc agttgccctg gcgcgccacc cgcgaccgct gggcggtgct cgtctccgag
     1801 gtgatgctgc accaaaccca ggcgtcgcga gtcgaggcgg cttggccgac gttcctcgcc
     1861 gagttcccga cccccgcagc gatggccgca gccgggcccg gcgacgtcat ccgcgcctgg
     1921 ggccgcctcg gctacccccg ccgagcccgg cgcctctggg aggccgcgtc ccgcatcgcg
     1981 agtaacggct ggcccgagga tctcaccgag ttgcccgggg ttgggcgcta caccgcaggg
     2041 gcgctccgcg cccaagccga cgacgccgac gtcggtgcgg tcgacgtcaa cattcgacgt
     2101 gtcgtggagc gggtcgtcgg acgaaggctc agt
//
LOCUS       DATCCH010000296         3899 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_322335, whole genome shotgun sequence.
ACCESSION   DATCCH010000296 DATCCH010000000
VERSION     DATCCH010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3899
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322335"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..1860)
                     /locus_tag="VIJ44_05675"
     CDS             complement(88..1860)
                     /locus_tag="VIJ44_05675"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY5165421.1"
                     /translation="MGTEVREAIPASSRPEVRRRRRWPWGSVSTDTSPVHHEMSSHRQ
                     VQLLWLVTGIMFVAVAATAPLVMRDARNDVHIATSRLVPASTALATAMRNYSDGLQEL
                     VVLVGTDPGARGNGITLLSTLNVAADTAWKNYTHFAANLPGEAKLQTQVRLEVSQLYA
                     TGATLLTTPTNSAAAVATVSALGAAQRKAMDRIQSLYQTRISAMVTHVNDDFGASQRD
                     LLIVSAFAFIIMMTGFALSVRTVRRRGQVQADEGRRNDLESGLQRALEMARTEDDCYR
                     LVQNAVERSSTTLSSELLVADSSRAHFHQVASTGPAGGPGCPVMSPDDCPSTNRGQTQ
                     TWSTSGALDTCPHLRDRDTGPCSAVCVPVSIAGKTIGVVHAIGPDHHPPDGSTTADLE
                     LIARKSGERIGILRAFSRTEAQARTDPLTGLLNRRSLETAVRDLAAEAHTYAIAYGDL
                     DHFKLLNDIHGHDAGDRALRLFARVLRDNVRPNDIAARYGGEEFVVVLPDCTVADAYV
                     VIDRVRTRLADAQRGGTVPSFTVSFGLAPGEADLAFGEIVEQADVALLRAKSEGRDRV
                     VLAGNRDEAPDAAPQPASELRPLS"
     gene            2091..2663
                     /locus_tag="VIJ44_05680"
     CDS             2091..2663
                     /locus_tag="VIJ44_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020662330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY5165422.1"
                     /translation="MPGTVHPVADERDGLLAYLAQQRSVIRITAYGLADDQARATPTA
                     STLSIGGLIKHVTAVENRWTDIDLQQSQPSADDYESNFRFGPDETLDGILADYDEGAR
                     RTEAVIAQIPDLGQALPVPQGVPWFPDDVDAWSVRWVLLHLIQETARHAGHADLVRES
                     LDGATAFPLMAAAEGWPATPWLQPWAPGDD"
     gene            2744..3112
                     /locus_tag="VIJ44_05685"
     CDS             2744..3112
                     /locus_tag="VIJ44_05685"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018332567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEY5165423.1"
                     /translation="MSVFSEAELTYLAEGKLGRIATIDPAGFPHVVPVGWEHNTELDT
                     IDIGGRNPEATQKFHNAQRQPKVAFVVDDVLPPWKPRCIMIRGTAETIPGATSGDPNG
                     GAIIRITPEKILSWGLDDQT"
     gene            3228..3536
                     /locus_tag="VIJ44_05690"
     CDS             3228..3536
                     /locus_tag="VIJ44_05690"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5165424.1"
                     /translation="MSEPAVLVADNPAASRYEIRVGDQLAGFAAYRTEPNRIIVTHTE
                     IDPTFKGRGLGNRLAAGTLDDLRRRHLAVTPQCPFMAKYIDEHPDYADLVAPISGTTT
                     "
     gene            3634..>3899
                     /locus_tag="VIJ44_05695"
     CDS             3634..>3899
                     /locus_tag="VIJ44_05695"
                     /inference="COORDINATES: protein motif:HMM:NF014148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HEY5165425.1"
                     /translation="MTPRITVVGGGSTHWTPRLLVDFANTPSLHNADVTLVDLDPDSL
                     PPMLDVAEHIAKCRPGIGLSASATTDLDAGLDGSDYVITAFSVGG"
BASE COUNT          631 a         1401 c         1294 g          573 t
ORIGIN      
        1 cgcgcgcgat ctccggcgtg cacatcccgt gagcggccgg gccttgagcg gccgggcctt
       61 gagcggccgg ggcgctcggg ccgggggtta ggagaggggg cgcagctcgg aggcgggctg
      121 gggcgctgcg tccggtgcct cgtcgcggtt gccggcgagg acgacccggt cgcggccctc
      181 ggacttcgcc cggagcagcg cgacgtcggc ctgctcgacg atctccccga aagccaggtc
      241 ggcctccccg ggcgcgagcc cgaagctgac cgtgaacgac gggacggtcc cgccgcgttg
      301 agcatcggcg agccgagtcc ggacccggtc gatgacgacg tacgcgtcgg cgaccgtgca
      361 gtcgggcagc acgacgacga attcctcgcc cccgtaccgg gccgcgatgt cattggggcg
      421 cacattgtcg cgcagcaccc gagcgaagag gcgcagggcc cggtcacccg cgtcgtggcc
      481 atgaatgtcg ttgagcagct tgaagtggtc gaggtccccg taggcgatcg catacgtatg
      541 tgcctccgcg gcgaggtcgc ggactgcggt ttcgaggctg cgccggttca gcagcccggt
      601 cagcgggtcg gtgcgagcct gggcttcggt gcggctgaag gcccgcagga tcccgatgcg
      661 ctcgccggac ttgcgggcga tgagctcaag gtcggcggtc gtggacccgt cgggcggatg
      721 gtggtcgggg ccgatggcgt gcaccacgcc gatcgtcttg cccgcgatgc tgaccggcac
      781 acacactgcc gagcagggcc cggtgtcgcg gtcccggaga tgcggacacg tgtcgagggc
      841 cccgctggtc gaccacgtct gggtctgccc tcggttggtg gacgggcagt cgtcgggaga
      901 catgacggga cagccgggac cgcccgcggg gccggtgctg gccacctggt ggaagtgcgc
      961 ccgactcgaa tcggcgacga gcagctcgga agagagggtg gtcgagctcc gctcgacggc
     1021 gttctgcacc agccggtagc agtcatcctc ggtgcgggcc atctcgaggg cccgttgcag
     1081 tcccgactcg aggtcgttgc ggcgaccctc gtcggcctga acctgtcctc ggcgccggac
     1141 cgtacgaacg cttagggcga acccggtcat catgatgatg aacgcgaatg cggagacgat
     1201 cagcaggtca cgctggctcg ctccgaaatc gtcgttcacg tgggtgacca ttgcgctgat
     1261 gcgagtctgg tagaggctct ggatccggtc catcgccttt cgctgtgcgg cgccgagtgc
     1321 gctgacggtc gccaccgcgg ccgccgagtt cgtcggcgtg gtgagcaacg tggcgccggt
     1381 cgcatacagc tggctgacct cgagtctgac ctgggtctgg agctttgcct ccccgggcag
     1441 gttggcggcg aagtgggtgt agttcttcca cgccgtgtcc gcggcgacgt tcaaggtgct
     1501 cagcaacgtg attccgtttc cgcgcgcacc gggatccgtc cccacgagca ccaccagctc
     1561 ctggagcccg tcggagtagt ttcgcatcgc ggtcgcgagc gccgttgacg cgggcacgag
     1621 gcgtgaggtg gcgatgtgca catcgttcct cgcgtcgcgc atgacgagcg gagcggtggc
     1681 ggcgacagcc acgaacatga tccctgtgac cagccacaag agctgcacct ggcggtgaga
     1741 cgacatctcg tggtgcaccg gggaggtgtc ggtgctgacg ctgccccagg gccaccgtcg
     1801 gcgacgtcgc acctcggggc gagaagacgc tggaatcgct tcccggacct cggtgcccat
     1861 actcctatgg tcgacacctc cgccctccaa cctgagggca gtcgtcctgg ccgacgctct
     1921 cgccggtaac gatctcaccg gctcggcgat cgcccgtgca gcacccgaat ccgggacccc
     1981 ggcgaccgtt ccgacgggcc ggccgtgggc cgccgagctc gaggccgccg ccagcatcgg
     2041 ccacgatggc ccgccaccca gatctggccc gacgaggagc gttgcccccg atgcctggaa
     2101 ccgtgcaccc ggtcgccgac gaacgcgacg gcttgctcgc ctacctcgct cagcagcgct
     2161 ccgtcatccg catcaccgcg tacggcctgg ccgacgatca ggcccgtgcc accccgaccg
     2221 cgagcaccct cagcatcggt gggctcatca agcacgtcac cgcggtcgag aaccgctgga
     2281 ccgacatcga cctccaacag agccagccat ccgcggacga ctacgagtcc aacttccgct
     2341 tcgggccgga cgaaaccctg gacggcatcc tcgccgacta cgacgaaggg gcgagacgca
     2401 ccgaggcggt catcgcccag atccccgact tgggtcaggc gttgccggtc ccgcaaggag
     2461 ttccctggtt ccccgacgac gtcgacgcct ggtcggtgcg atgggtgctg ctccacctca
     2521 tccaagagac cgcccgccat gccgggcacg ccgacctcgt ccgagagtca ctcgacggcg
     2581 cgaccgcctt cccgctcatg gccgccgccg aggggtggcc agccacccca tggctccaac
     2641 cgtgggcccc gggagacgac tgaccaccgg cgagcgggca ccaccccgcc cgcatgggaa
     2701 agtgacggcc gtccggcatc gacaaccacg agggctgcgt cccatgagcg tcttctccga
     2761 ggctgagctc acctacctgg ccgaaggcaa gctgggtcgc atcgccacca tcgaccccgc
     2821 gggctttccc cacgtcgttc ccgtcgggtg ggaacacaac acggagctcg acacgatcga
     2881 catcggtggc cgcaaccccg aagccaccca aaagttccac aacgcccagc gacagcccaa
     2941 agtcgcgttc gtcgtcgacg acgtcctccc gccctggaag ccccggtgca tcatgattcg
     3001 cgggaccgcc gaaacgatcc cgggcgccac cagcggcgac cccaatgggg gagcgatcat
     3061 ccgaatcacc cccgagaaga tcctgagctg gggcctcgac gaccaaacct gacgatctcg
     3121 ctcccacccg accagagccg cggccgccaa ctcggaggtg agagcatctc gtcgccgccc
     3181 cacccggctc cgatatggac gcgggagacc cgccgatcga ggtgacgatg agcgaaccgg
     3241 ccgtgctcgt ggccgacaac cccgcggcgt ctcgctacga gatccgagtc ggcgaccagc
     3301 tggccgggtt cgccgcttac cgcaccgagc ccaaccggat catcgtcacc cacaccgaga
     3361 tcgacccgac gttcaagggt cgcggcctcg ggaaccgcct cgccgccggg accctcgatg
     3421 acctgcgccg ccgacacctc gcggtgacgc cccaatgccc gttcatggcc aaatacatcg
     3481 acgagcatcc cgactacgcc gacctcgtcg cgcccatctc tggcacgaca acctgagacc
     3541 gaccgatcgc gacgcgacca acggctcggt cgtcggagcc aaggtgacgc ggtcctcgca
     3601 ccgctatcgt cccccgcgag gaggacaacg acgatgacac cgcggatcac cgtggtcggg
     3661 ggcggctcga cgcactggac cccacggctc ctggttgact tcgcgaacac accgtcgctg
     3721 cataacgccg atgtcaccct cgtcgacctt gaccccgact cgcttccccc gatgctcgac
     3781 gtcgccgagc acatcgcgaa atgccgtccg ggaatcgggc tctcggcgtc agcgacgacc
     3841 gacctcgatg ccggcctcga cggatccgac tacgtgatca ccgcgttctc ggttggcgg
//
LOCUS       DATCCH010000297         3331 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_322340, whole genome shotgun sequence.
ACCESSION   DATCCH010000297 DATCCH010000000
VERSION     DATCCH010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322340"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1813
                     /locus_tag="VIJ44_05700"
     CDS             <1..1813
                     /locus_tag="VIJ44_05700"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEY5165426.1"
                     /translation="MVFQNTATAILVGFGFGGSLLALFMRVGGGIFTKAADVGADLVG
                     KVEQGIPEDDPRNPAVIADNVGDNVGDCAGMAADLFESYEVTLVAAIILGVPAFASIG
                     ANPALGLIFPLIARAVGVLASIVGVFMVRATGRDRSAMAPINRGFATAGILTILGVGA
                     VGVFYVGNNHGSEGWRVVGAVVIGLLLAQVVSRLTEYFTSTETQPVQEIAEATRTGPA
                     TTILSGISSGLESTVWSILAIAIAIGSAIALGNGNLQFSLYLIALTGMGMLATTGVVV
                     SEDTFGPVSDNAAGIAEMSGEFEGEPQRIMVSLDAVGNTTKAVTKGFAIGSAVIAAVA
                     LFASYIETIGTELGIKKVGAALFRDPRAAVNVADPKTFIGLLVGGSIAFLFSALAIRA
                     VGRSAGTVVQEVRKQFRERRIMEGIDQPEYGKVIDICTTAALRELTTPALLAVLSPVI
                     IGFGISYLALGAFLAAVILTGQLMAVFLSNAGGAWDNAKKYIEDGHYGGKGSESHTAA
                     VIGDTVGDPFKDTAGPALNPLIKVMNLVSLLLLPAIISLRNDDVARFAISGVALVVLL
                     CAIAFSKRRGGAFADESDEVNEALTTSGSPEGASAGE"
     gene            complement(1887..3101)
                     /locus_tag="VIJ44_05705"
     CDS             complement(1887..3101)
                     /locus_tag="VIJ44_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEY5165427.1"
                     /translation="MIFVGVDWSEDHHDIDIRDEAGERLATLRVSHGVEGIARVHAAI
                     GEHAVDPGGVAVGIETDRGLLVAALVGADYQVYAINPRSVDRYRDRHGLSGAKSDAGD
                     ARVLAELVRTDRHHHRLVAGDSDELEGLRMLARNHQNLIWARVRHVNQLRSLLLEYYP
                     AALEAFAGQLTSRDALAVLAAAPHPETGRRLRRSELVALLRAGGRVRNLEDRAEQIHA
                     ALTNPQLVPSPVFADACAASASALVGLITALNTQIDGLQALLAEHFEQHPDAEIVRSL
                     PGLGNILGARVLSEFGDDPDRYTDAKARRNYAGTSPVTRASGKSKVVLARFVRNRRLA
                     DACDRWAFAALTASPGARRYYDEHRARGNTHRQALRTLSNKLVGILHGCLTTNTTYNE
                     DTAWRRYHATAA"
BASE COUNT          517 a         1126 c         1120 g          568 t
ORIGIN      
        1 catggtcttc cagaacaccg ccaccgccat cctcgtcggg ttcgggttcg gtggctcgct
       61 gctcgccctg ttcatgcggg tcggcggcgg catcttcacc aaagccgccg acgtcggcgc
      121 cgacctcgtc ggcaaggtcg agcagggcat ccccgaggac gatccgcgca acccggcggt
      181 catcgcggac aacgtgggcg acaacgtcgg cgactgcgcc gggatggctg ccgacctctt
      241 cgagtcctac gaggtgacgc tcgtcgccgc catcatcctt ggcgtccccg cgttcgcgtc
      301 gatcggcgcg aaccctgccc ttggtctgat cttccccctc atcgcccggg ccgtcggtgt
      361 cctcgcctcg atcgtcgggg tgttcatggt ccgggccacg ggccgggacc gctccgcgat
      421 ggcgcccatc aaccgcgggt tcgccaccgc ggggatcctg accatcctcg gggtcggggc
      481 cgtcggggtc ttctatgtcg gcaacaacca cggcagcgaa ggctggcggg tggtcggcgc
      541 ggtggtgatc ggactcctgt tggcccaggt cgtcagccgc ctcaccgagt acttcacctc
      601 caccgagacc cagccggtgc aagagatcgc cgaggccacc cgcaccgggc ccgcgaccac
      661 gatcttgtcg ggcatcagca gcggcctcga gtcgaccgtc tggtcgatcc tcgccatcgc
      721 catcgcgatc ggcagcgcca tcgcgctcgg caatgggaac ctgcagttct cgctctacct
      781 gatcgcgctc accggcatgg gcatgctcgc gaccaccggc gttgtcgtct ccgaggacac
      841 cttcggaccg gtctccgaca acgccgcggg catcgccgag atgtcgggcg agttcgaagg
      901 cgagcctcag cggatcatgg tcagcctcga cgccgtcggg aacacgacga aggcggtcac
      961 gaagggcttc gcgatcgggt ccgcggtcat cgccgcggtc gcgctcttcg cgtcctacat
     1021 cgagacgatc gggaccgagc tcggtatcaa gaaggtcggg gcagccctgt tccgagaccc
     1081 tcgggccgcg gtcaacgtcg ctgacccgaa gaccttcatc gggctcctcg tcggcggttc
     1141 catcgcgttc ttgttctcgg cgctcgccat ccgcgccgtc ggtcgctccg cgggaaccgt
     1201 ggtccaagag gtccggaagc agtttcgcga gcggcgcatc atggagggca tcgaccagcc
     1261 ggagtacggc aaggtcattg acatctgcac gaccgcggca ctgcgcgagc tgaccacccc
     1321 ggcgctgctc gcggtgctca gcccggtgat catcgggttc gggatcagct atctggcgct
     1381 cggcgcgttc ctcgcggcgg tgatcctcac cggccagctc atggccgtgt tcctgtccaa
     1441 cgcgggtggc gcgtgggaca acgccaagaa gtacatcgaa gacggccact acggcgggaa
     1501 gggctccgag tcccacaccg ccgcggtcat cggcgacacg gtgggcgacc cgttcaagga
     1561 caccgccggt ccggccctga acccgctcat caaggtcatg aacctcgtgt cgctcctgct
     1621 cctcccggcg atcatcagcc tccgcaacga cgacgtcgcc cgcttcgcga tctccggggt
     1681 ggccctggtt gtgctcctgt gcgcgatcgc gttctcgaaa cggcgtggcg gagccttcgc
     1741 cgacgagtcc gacgaggtca acgaggcgct cacgacatcc gggagccccg agggcgcatc
     1801 cgccggcgaa tagccacctc cggcctccgc gagcgcaccc gcgccgtgcg gtcggacgcc
     1861 tcgtagacat cccaggggtt tgggtgtcaa gcggcggtgg cgtggtagcg gcgccaggct
     1921 gtgtcttcgt tgtaggtggt gttggtggtg aggcagccgt gaaggatgcc gacgagcttg
     1981 ttggagaggg tgcggagtgc ttggcggtgg gtgttgcctc gtgcgcggtg ttcgtcgtag
     2041 taacggcggg cccccggtga ggcggtgagc gcggcgaagg cccagcggtc gcaggcgtcg
     2101 gcgaggcggc ggttacgcac gaagcgagcg aggacgactt tgctcttccc ggacgctcgg
     2161 gtgaccgggg aggtgccggc gtagtttcgg cgggctttag catcggtgta gcggtcgggg
     2221 tcgtctccga actcgctgag cacccgggcg ccgaggatgt tgccgagtcc aggaagactg
     2281 cgcacgatct cggcgtccgg gtgttgttca aaatgttctg cgagcagtgc ttggagcccg
     2341 tcgatctggg tgttgagcgc agtgatgagc ccgacgagcg cgctggcgct ggcggcacag
     2401 gcgtcggcga acaccggcga gggcaccagt tgcgggttcg tcaacgcggc gtggatctgt
     2461 tcggctctgt cctcgaggtt gcggaccctt ccaccagctc ggagcagcgc cacgagctcg
     2521 gatcgacgaa ggcgacggcc ggtctcggga tggggcgcgg cggcgagcac cgcgagggca
     2581 tcgcggctgg tcaactggcc ggcgaaggct tccagcgccg ccgggtagta ctccaacaac
     2641 aggctgcgga gctggttgac gtgacggacc cgcgcccaga tcaggttctg gtggttgcga
     2701 gccagcatcc ggagcccctc gagctcgtcg ctgtcaccgg cgacgagacg gtgatgatgc
     2761 cgatcggttc gcaccagctc ggcgagcacc ctggcatcgc cggcgtcgga cttggcgcct
     2821 gagagcccgt gtcggtcccg ataccgatcc actgaccggg ggttgatcgc atacacctgg
     2881 tagtcagcac ccaccagagc cgccaccaac agcccccggt cggtctcgat ccccaccgcg
     2941 accccgccgg gatcgaccgc gtgctcgccg atcgccgcgt gcacgcgggc aatcccctcg
     3001 acaccatgcg agacccgcaa cgttgccaaa cgctcaccgg cttcgtcgcg gatatcgatg
     3061 tcgtggtggt cctcagacca atccaccccg acgaagatca tggaacctcc cttgtcgtca
     3121 tgtctgtttg agccgaggaa cacgccgacg gcctaatgga ccagtactcg aagtacgaca
     3181 tcccagaagt ggtcatcagt tcctcaccaa ccgcgggggc acggtctacg agtagaactc
     3241 acagctcggg ctgtcccagt gctcaccctc gggcggctcc atcacagcct tacctcacaa
     3301 cgaagcagct agatggaccc ccattaggct g
//
LOCUS       DATCCH010000298         2081 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_198993, whole genome shotgun sequence.
ACCESSION   DATCCH010000298 DATCCH010000000
VERSION     DATCCH010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198993"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VIJ44_05710"
     CDS             <1..306
                     /locus_tag="VIJ44_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165428.1"
                     /translation="EGSQGISEREFNGPVRGWQDEYRAGVAALGSDFATLPGEDQDAR
                     LDAGPEFKALLYGHACEGAYGAPEYGGNRDGRGWTAIQYPGDVQPQGYTDDEVAGRG"
     gene            316..2025
                     /locus_tag="VIJ44_05715"
     CDS             316..2025
                     /locus_tag="VIJ44_05715"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEY5165429.1"
                     /translation="MTDVDAVIIGSGPAGATAAEVLTAAGWSVTILEKGRNHLLSLDS
                     PFEPLGHVSNDEVKFLRRHVLGPDPFLEPRTFRRRVDDGDRILTGGVNNLPSTVGGGG
                     FHADGKLPRFRTVDFHARSELGPIEGSDIVDWPFGYDELEPYYTAAEAVVGVAGDATG
                     NPFASWRSGPYPMPPGPDMFCAVITSDAAGRHGYHPYRAPTGVNSVEYDGRPACNNCG
                     FCAFFGCPIEAKGDPVAPLRHALRTGRCEIRPESYVTEIVLDPGGRQARGVRYLDAHG
                     TEHEVTARHVVLGAGAFETPRLLLRSGIGHPDIVGRYLMFHFQTYVLGIFPFRLHSHR
                     GRSVTHLMDDPIVPDAAAQQAARDAGLPYLRGGIVEHGGSSHPILEAVHLPAGDDHTR
                     LMLASPMRDRMAAFTMQGEDLPQATNRVDLDPAVRDVWGTPAGRVTYAPHRHEIACAE
                     HWAPRLEAVMRDAGARHTFWVTSPPLEGSRAAQGFDPTPISRHIMGTARMGDDPRRSV
                     CDPWQRLHDVDNVVITDSSVFPTSTGYGPTLTIVALAIRASRALAGLAPLRSTPPTDA
                     QRA"
BASE COUNT          300 a          784 c          704 g          293 t
ORIGIN      
        1 gaagggtcac agggcatctc cgaacgcgag ttcaacggcc cggtgcgggg ctggcaagac
       61 gagtaccggg ccggcgtcgc ggccctcgga tcggacttcg cgacgctccc cggcgaggac
      121 caagacgccc gtctcgacgc cggccccgag ttcaaggcgc tgctctacgg ccacgcctgc
      181 gaaggtgcct acggcgcccc cgaatacggt ggcaaccgcg acgggcgcgg ctggaccgcg
      241 atccagtacc ccggcgacgt gcaaccccag ggctacaccg acgacgaggt cgccggtcgt
      301 gggtgagagc cggccgtgac cgacgtcgac gcggtgatca tcgggagcgg ccctgcgggc
      361 gcgaccgcgg ccgaggtgct caccgcggcc ggctggtcgg tcaccatcct cgagaagggc
      421 cgcaaccacc tcctcagcct ggattccccg ttcgagccac tcggccatgt cagcaacgac
      481 gaggtcaagt tccttcgccg ccacgtcctg ggccccgacc cgttcctgga gccccgcacg
      541 ttccgtcgac gcgtcgacga cggcgaccgg atcctcaccg gcggggtgaa caacctgccg
      601 tcgacggtgg gcgggggcgg gttccacgcc gatgggaagc tgccccggtt ccgcaccgtg
      661 gacttccacg cccgatcgga gctcggtccg atcgagggtt ccgacatcgt ggactggccg
      721 ttcggctacg acgagctcga gccgtactac accgcggcgg aggcggtcgt cggcgtcgcc
      781 ggtgacgcga ccgggaaccc gttcgcgtcg tggcgatccg gcccgtaccc gatgcccccc
      841 ggtccggaca tgttctgcgc cgtcatcacc tccgacgccg cgggccgaca cggctatcac
      901 ccgtaccggg caccgacggg ggtcaacagc gtcgagtacg acggccggcc ggcgtgcaac
      961 aactgcgggt tctgcgcctt cttcggctgc cccatcgaag ccaagggcga cccggtcgcg
     1021 cctctgcgcc acgccttgcg caccgggcgc tgcgagatcc gccccgagtc gtacgtgacc
     1081 gagatcgtcc tcgaccccgg tggacgccag gcccgcgggg tccgctatct cgacgcgcac
     1141 gggacggagc acgaggtcac cgctcgtcac gtggtgctcg gcgcgggcgc gttcgagacc
     1201 ccccggctgc tcctccgatc gggcatcggt catcccgaca tcgtggggcg ctacctcatg
     1261 ttccactttc agacctacgt cctcgggatc ttcccgttcc gccttcactc ccaccgcggc
     1321 cggtccgtca cccacctgat ggacgacccg atcgtccccg acgccgcggc ccagcaggcc
     1381 gcgcgggacg ctggcctccc ctatctgcgg ggaggaatcg tcgagcacgg tggaagctcg
     1441 cacccgatcc tcgaggcggt ccacctcccg gccggtgatg accacacccg actcatgctc
     1501 gcctcaccga tgcgggaccg catggccgcg ttcacgatgc agggcgagga tctgccgcag
     1561 gccacgaacc gggtggacct ggaccctgcg gtccgggacg tgtggggcac gccggcggga
     1621 cgggtcacct acgcgcccca tcgtcacgag atcgcctgcg ccgagcattg ggccccgcgc
     1681 ctcgaggcgg tcatgcgcga cgccggggcg cgccacacct tctgggtgac gtctccaccg
     1741 ctggagggtt cgcgggccgc gcaagggttc gacccgaccc cgatctcccg tcacatcatg
     1801 ggcaccgccc ggatgggtga cgaccctcgg cgcagcgtct gtgatccgtg gcagcgcctc
     1861 catgacgtcg acaacgtcgt gatcaccgat tcgtcggtgt tcccgacatc gacggggtac
     1921 gggccgaccc tgacgatcgt ggcgctggcc atccgcgcct cgcgggcact cgcggggctg
     1981 gcgcccctgc gctcgacgcc gccgaccgac gctcagcggg cgtagaggcc gacgagctcg
     2041 gcccggccga ggacgacgcc ccggaccgcc gagcgaagct g
//
LOCUS       DATCCH010000299         2000 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_489880, whole genome shotgun sequence.
ACCESSION   DATCCH010000299 DATCCH010000000
VERSION     DATCCH010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489880"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="VIJ44_05720"
     CDS             complement(<1..619)
                     /locus_tag="VIJ44_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011603140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY5165430.1"
                     /translation="MTEQLYFRQLLSGHDFARESPVAAQMVNFVYLIGDRETGEAVAV
                     DPAYAVQELVDLLAADGLRLTGVLATHWHPDHIGGDLMGYGIEGIQELLGTPDVSARV
                     HIQRDEADWVKRATGASDRDLVLHDSGDTVEVGRIPIRLIHTPGHTPGSQCFVVDGKL
                     VAGDTLFLDGCGRTDLSGGDADQMYESLTQRLATVPDDTVLYPGHR"
     gene            715..>2000
                     /locus_tag="VIJ44_05725"
     CDS             715..>2000
                     /locus_tag="VIJ44_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020542597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY5165431.1"
                     /translation="MSSVAYRTCPLCEATCGLELHLEERDITLVRGDRDDVFSHGFLC
                     PKGSAIGRIDTDPDRVRQPLVRDGDEWHKVSWATAFAVIERRLTPILDAHGRDAVALY
                     VGNPNAHNLAGLIYNRALIQALGSRNVYSASTLDQMPKQVSAGLMFGAALSVPVPDVD
                     RTDHLLLLGANPFASNGSLLTAPDLPGRLRALRARGGKLVVIDPRRTKTADEADEHLF
                     IRPGTDAAFLAAVAHTLLDEGLTDPGRLRDHVNGWATLEALVTDFTPEAVAVWCGIDA
                     ATIRRVARELAAAPHAAVYARLGTCANEFGTVASWLVDVCNVLTGNLDREGGAMFARP
                     AAGGANTGGEPGVGRGVRFGRRNSRVRGLPEVYGELPVVCLAEEIETPGDGQVRAVVT
                     VAGNPVVSAPDSARLDRAFGGLEFMVSVDIYQNETTR"
BASE COUNT          293 a          685 c          693 g          329 t
ORIGIN      
        1 accggtgacc ggggtagagg acggtgtcgt ccgggacggt ggccagccgt tgggtgaggc
       61 tctcgtacat ctggtcggcg tcgccgccgg acaaatcggt ccggccgcag ccgtcgagga
      121 agagggtgtc gccggccacg agcttgccgt cgacgacgaa gcattggctc ccgggggtgt
      181 ggccgggagt atggatgagc cggatcggga ttcggccaac ttcgacggtg tcgccgctgt
      241 cgtgcaggac caggtcgcga tcgctggccc cggtggcgcg cttcacccag tcggcctcgt
      301 cgcgttggat atggacgcgt gccgacacat cgggtgttcc cagcagctct tggattccct
      361 caatcccgta acccatcagg tcgccgccga tgtggtccgg gtgccaatgg gtggcgagca
      421 ccccagtgag ccgcagccca tcggcggcga ggaggtcgac gagttcctgg actgcgtacg
      481 cagggtcgac ggcgacggcc tcgccggtct cgcggtcccc gatcaggtac acgaagttga
      541 ccatctgggc ggcgaccggg ctctcccggg caaagtcgtg gccggacagc agctggcgga
      601 agtagagctg ttccgtcacc gagaatcgct cgccgcggcc atgaaacgag tctttctgct
      661 gtcggcttcg agtcggggcg cagcgtagtg gccagacgcg tagcctctcg gggcatgtcg
      721 agcgtcgcgt accgaacctg tccactttgt gaggcgacct gcggcctcga gcttcatctc
      781 gaggagcgcg acatcaccct ggtgcggggc gaccgtgacg acgtgttcag ccacgggttc
      841 ctctgcccga agggcagcgc gatcggacgg atcgacaccg accccgaccg tgttcgccaa
      901 cccctggtcc gcgatggtga cgagtggcac aaagtcagct gggccaccgc gttcgctgtg
      961 atcgagcgcc gcctgactcc gatcctcgac gcccacgggc gcgacgcggt ggcgctctac
     1021 gtgggcaacc ccaacgctca caacctcgct ggtctcatct acaaccgggc cctgatccaa
     1081 gcgctcggtt cgcgcaacgt ctactcggcc agcaccctcg accagatgcc gaagcaggtg
     1141 tcggccgggc tcatgttcgg tgccgcgctg tcggtgccgg ttcccgacgt cgaccgcacc
     1201 gaccatctct tgttgctggg cgccaacccg ttcgcgtcga acggcagcct cctcaccgcc
     1261 cccgatctcc ccgggcgact ccgtgcgctt cgggcccggg gcgggaagct cgtggtgatc
     1321 gaccctcgcc gcaccaagac cgccgacgaa gcggacgagc acctcttcat ccgacccggt
     1381 accgatgccg cgttcctggc cgcggtggct cacaccctgc tcgacgaggg tctcaccgac
     1441 cccggtcggc tgcgggacca cgtcaacggc tgggcgacac tcgaggccct cgtcacggac
     1501 ttcactccgg aggcggtcgc ggtctggtgc gggatcgacg cggcgacgat tcgccgcgtg
     1561 gcccgcgagc tcgccgcggc tccccacgcc gcggtgtacg cccgcctcgg cacctgcgcc
     1621 aacgagttcg gcaccgtggc gtcctggctg gtggacgtgt gcaacgttct gaccggcaac
     1681 ctggaccggg aaggcggtgc catgttcgcc cgcccggctg cgggcggcgc gaacaccggc
     1741 ggcgagcccg gtgtcgggcg aggcgtccgg ttcggccgtc gcaacagccg ggtccggggt
     1801 cttcccgagg tgtacggaga gctgccggtc gtctgtctcg ccgaggagat cgaaaccccc
     1861 ggtgacgggc aggtgcgagc cgtcgtgacc gtcgcgggca acccggtggt ctcagcgccg
     1921 gacagcgcgc gtttggatcg cgcgtttggc gggctcgagt tcatggtcag cgttgacatc
     1981 taccagaacg agaccactcg
//
LOCUS       DATCCH010000300         2141 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_282050, whole genome shotgun sequence.
ACCESSION   DATCCH010000300 DATCCH010000000
VERSION     DATCCH010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282050"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="VIJ44_05730"
     CDS             complement(<1..809)
                     /locus_tag="VIJ44_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009326588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY5165432.1"
                     /translation="MLLMRLGPSGAERPGVRINGDTYVDVSDVVKDYDEAFFAAGAAG
                     LADLRTLVEQRVADGRTTPFAGERVGAPIARPHQILCIGLNYSDHAAETGQAVPAEPI
                     LFTKAPNTLVGPYDDVRLPRGSTKVDWEVELGIVIGRRSSYLADAAAARDSIAGYVLV
                     NDVSERAFQLERGGQWSKGKSAETFNPAGPWLATTDEIADPMNLTLTLDVNGIRRQTG
                     STATMIFDPYFLVHYLSQFMVLEPGDLINTGTPPGVGMGARPPTYLRVGDV"
     gene            complement(844..916)
                     /locus_tag="VIJ44_05735"
     tRNA            complement(844..916)
                     /locus_tag="VIJ44_05735"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(881..883),aa:Arg,seq:cct)
     gene            complement(941..>2141)
                     /locus_tag="VIJ44_05740"
     CDS             complement(941..>2141)
                     /locus_tag="VIJ44_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEY5165433.1"
                     /translation="GHLQGTIRDATTHLPLAGICVSSRLIPDPTFSGSGQGCTDALGH
                     YDIAAKAGAYQLEAIDFSGAFLHRWYGGSLNQSAPATKTVHLAANSVLGHLDVSLVAA
                     GHITGVAVDATTGLPLANTALRVHLGRDPQEAEAYGNTYTDASGVFDVGGLPTGSYTL
                     EATSNDRIHAPQWYGGQGTQASAAPIHVTVGRSTTIGSLQIPVGGTIRGRVTDRVSGA
                     PIANVCVGVGIWNYRSGDDGSGYGEACTDSTGRYALTGLARGSYFVEYVPQFLAYAPT
                     WFHSFHRTLSTPVAVMDGKTVLANQSLVIGATVTGTITGLAAPDQTQFDAFDPITGDP
                     VGRGTAFPDGTWSITGIPPGGTKVAADAYSPAVIWWRHATTIGGAAVISTTSGHTHAG
                     IDFAFTP"
BASE COUNT          308 a          708 c          779 g          346 t
ORIGIN      
        1 atgacgtccc cgacccgcag gtacgtcggt ggcctggcgc ccatgccgac cccgggcggc
       61 gtacccgtgt tgatcaggtc accaggctcg agcaccatga actggctgag gtagtgcacg
      121 aggaagtacg ggtcgaagat catcgtggcg gtcgacccgg tctgccggcg aatgccgttg
      181 acgtcgagcg tgagcgtcag gttcatcggg tcggcgatct cgtcggtcgt ggcgagccac
      241 ggtccggccg ggttgaacgt ctctgcggac ttccccttcg accactgacc cccgcgctcc
      301 agctggaacg cgcgctcgct gacgtcgttg accaggacgt agccggcgat cgagtcgcgg
      361 gccgctgcgg cgtcggcgag gtagctgctg cgccggccga tgacgatgcc cagctcgacc
      421 tcccagtcca ccttggtcga gccacgtggc agtcgtacgt cgtcgtacgg accgacgagc
      481 gtgttgggcg ccttggtgaa caggatgggt tcggctggca ccgcctggcc cgtctccgcc
      541 gcgtggtcgc tgtagttgag gccgatgcac aggatctggt gcggccgcgc gatcggtgcg
      601 ccgacacgct cgcccgcgaa cggggtggtc cggccgtcag cgacacgttg ctccaccagg
      661 gtgcgcagat ccgccagccc cgcggcaccg gcggcgaaga acgcctcgtc gtagtccttg
      721 accacgtcgg agacatcgac ataggtgtcg ccgttgatgc gcacgcccgg ccgttcggca
      781 ccggaggggc ccagccgcat gaggagcatg ggtgtggtcg ccctttctcg ggaggcggag
      841 gcgtgccctc gggcagattc gaactgccga cacccgcttt aggagagcgg tgctctatcc
      901 cctgagctac gagggcgggg aacgccaggg tatctggcgg tcagggcgtg aacgcgaagt
      961 cgatgcccgc gtgggtatgt cccgacgtcg tcgagatgac cgcggcgccg ccgatggtgg
     1021 tcgcgtgccg ccaccagatg acggccgggc tgtaggcgtc ggcggcgacc ttcgtaccgc
     1081 ccgggggtat gccggtgatg gaccacgtac cgtccggaaa cgcggtgccc cgaccaacag
     1141 ggtcgcccgt gatcgggtcg aaggcgtcga actgggtctg gtccggagcg gcgagcccgg
     1201 tgatggtgcc ggtcacggtc gcaccgatca ccagcgactg gttggccagg accgtcttcc
     1261 cgtccatcac ggcgaccggc gtcgacaacg tccggtggaa gctgtggaac caggtaggtg
     1321 cgtacgccag gaactgcggc acgtactcga cgaagtagct gccccgcgca agccccgtca
     1381 gcgcgtaacg gccggtgctg tccgtgcacg cctcgccata gccggagccg tcgtcccccg
     1441 agcggtagtt ccagatgccg acgccgacgc acacgttcgc gatcggcgct ccgctgacac
     1501 ggtcggtcac ccggcctcgg atcgtccctc ccacggggat ctgcagcgat ccgatcgtgg
     1561 tcgaccgtcc gaccgtgacg tggatgggcg cggcgcttgc ctgggtgccc tgcccgccgt
     1621 accactgcgg cgcatggatg cggtcgttcg acgtcgcctc caaggtgtag ctgcccgtcg
     1681 gcagcccacc cacgtcgaag acgccactgg catcggtgta cgtgttcccg tacgcctccg
     1741 cctcctgcgg gtcgcgcccg aggtgcaccc ggagggcggt gttggcgagg ggcagacccg
     1801 tcgtggcgtc gaccgcgaca ccggtgatgt gcccggccgc gacgagtgag acgtcgaggt
     1861 gcccgagcac gctgttcgcg gccaggtgga cggtcttggt ggccggggcg gactggttga
     1921 gggacccgcc gtaccaccgg tgcaggaatg ctccgctgaa gtcgatggcc tcgagctggt
     1981 aggccccagc cttggccgcg atgtcgtagt ggcccagcgc gtcggtgcac ccctggcccg
     2041 acccgctgaa cgtggggtcc gggatgagcc gggaggagac gcagatgccg gcaagtggca
     2101 ggtgcgtcgt cgcgtcgcgg atggtgccct gcaggtggcc g
//
LOCUS       DATCCH010000301         4717 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_531030, whole genome shotgun sequence.
ACCESSION   DATCCH010000301 DATCCH010000000
VERSION     DATCCH010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4717
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531030"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="VIJ44_05745"
     CDS             complement(<1..352)
                     /locus_tag="VIJ44_05745"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5165434.1"
                     /translation="MNELHPPIAAPLGDVTTGRLVLRRFDRDVLDELACVFENREVWQ
                     FPYGRGMTRAETVVFLDAQIRHWEHFGFGCWTARAREDERLIGYVGLSIPKFLPEILP
                     AVEVGWRFAPSAWGN"
     gene            complement(406..927)
                     /locus_tag="VIJ44_05750"
     CDS             complement(406..927)
                     /locus_tag="VIJ44_05750"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HEY5165435.1"
                     /translation="MAFSKPSTVVPTPDERELLLRFLNRQREEVVAVAEGLSDEQACW
                     TPDGALLSVVGVINHLTHVECRWVEGRYLGAPFPERTDEFAADGIGVRQACTAYWVQA
                     QRTDDVVRAAPSLEVACLGREGDWPPAHTLLGLDEPLDLRWVLLHLIEETAHHAGHAD
                     ATRELLDGKIMRP"
     gene            1037..2674
                     /locus_tag="VIJ44_05755"
     CDS             1037..2674
                     /locus_tag="VIJ44_05755"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEY5165436.1"
                     /translation="MEPEPRPHDLDHGSRAPGAQTGQQPWIASWTRRRFLTGAGVAAA
                     GLVGGLGAACSGSGGASARKPVPRSTPSSTAPAKPDSAGIVVGDLPSEEQVFGWIKEV
                     VSHGIRRPGYPADVWAEGWIQQQFRDMGLENVHREAVPLRSWKPGTSSLRATTATGET
                     RDIEHFPVPYAAPVRDLDIELVAYDAAHLSAVAAKASLYTVKLITLPATALARSGGVP
                     KDMTGRIVDDAEQSLAKGQHTVPFAPEFQDVAGPSIKAGAAAFIGTLTASPGDTYEYY
                     VPYTSDELPIPGVWISASSGNWLHDQLAKGPVRIVLTVDSTVTDHTSYNIVGDLPGAD
                     EEVVIVASHHDGPWASAVEDGSGTSLVLAQAHYWSRRPRAERPHRLRFILQAGHMCGG
                     AGMLAYIKAHGEELGNVVLEVHLEHAALEAEEDSGGKLVTTDRAVPRWFFTSRHATLE
                     KAVSNALTGEQLTRSMVMAPDAFGSAPPTDGQGYFAAGVPAVNFLTAPWYLFDSVDTL
                     DKIDRAHLVPLTRATIRIIKFTAETTSAKLRAEPPTP"
     gene            complement(2683..4302)
                     /locus_tag="VIJ44_05760"
     CDS             complement(2683..4302)
                     /locus_tag="VIJ44_05760"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEY5165437.1"
                     /translation="MSSARPTLGLGFHSAIIAPELSPRTPIAATPDGSRATVRRPNGG
                     LEKGRAMRFGNPRLKALRGHRVPAWWSDAKLGIFVHWTPASVPAFAPVDVDIGELMQS
                     GRRDALAFCPYAEWYENSLRFPDSPVARHHREVYGNRPYVQFADDWAAGLEHWDPHEW
                     AARFAATGAKYVVFVTKHMDGYCMWPTNVPNPRRPGWNCGRDVVGELCEAVRGAGMRF
                     GIYYSGGLDSTFNDRPIGSLSEMLDAIPRGDYPAYAEAQVRELIARYRPSVLWNDIAW
                     PAEGKQLWPLFEHYYAQVPDGVVNDRWMPWSPLLAAARSQLGRRAIDAGVRHQAKRDA
                     GVVPPVPPHSDVRTPEYVVFDDVQRKPWECVRGMDQSFGYNACSRPEHFLAHDDLLWM
                     LTDIVSKGGNLLLNVGPRGVDARIPDEQLTRLEWLAEWVTPHAAAIAATRPWVTPGTT
                     TPDGQPVRYTARDETVYAFVQDATGSITLPDVCATPTTAVTTVTGTALSWNDSPSGLV
                     VDTPPAAARPDPTVLALSHVAARSSTTLTER"
BASE COUNT          794 a         1616 c         1556 g          751 t
ORIGIN      
        1 cgttgcccca tgcagacggc gcgaaccgcc agccgacctc gactgccggg aggatctcgg
       61 gcaggaattt tgggatcgaa aggccgacgt agccgatcag gcgttcgtct tcacgcgctc
      121 tcgccgtcca gcatccgaat ccgaagtgtt cccagtgccg tatctgcgcg tcgaggaaga
      181 ccaccgtttc ggcgcgagtc atccctctcc cgtaggggaa ctgccagacc tcacggtttt
      241 cgaagacgca cgcaagttcg tcgagaacgt cacgatcgaa tcgccgaaga accaggcgcc
      301 cagttgtgac gtcacccaag ggtgccgcga tcggcggatg aagctcgttc atccacacag
      361 cttgcccggc gaggcgcccc cgaacgcgcg tcccggaatc ccgggtcagg gtcgcatgat
      421 cttgccgtcg agaagctccc gcgtggcatc ggcgtgtccc gcgtggtgcg cggtctcctc
      481 gatgaggtga agcagcaccc agcgcagatc cagcggttcg tcgagcccga gcaacgtgtg
      541 cgcgggaggc cagtcccctt ctcggccgag gcatgcgacc tccaggctcg gagccgcccg
      601 cacgacgtca tccgttcgct gcgcctgcac ccagtacgca gtacaagcct gcctcacgcc
      661 gatcccgtcg gctgcgaact cgtcggtacg ttccgggaac ggcgccccga gatagcggcc
      721 ttcaacccag cggcactcga cgtgggtgag atggttgatg acgccgacga ccgacaacag
      781 ggcaccgtcc ggcgtccaac acgcctgctc gtccgacagc ccttcggcaa cggccacgac
      841 ctcttcgcgc tgacgattca ggaaccgcaa cagcagttcg cgctcgtccg gagtcggcac
      901 caccgtgctc ggcttcgaga acgccacgag cgcatcatgc ccgccgcggg acgcttgtgc
      961 gagcgcagat cggcgcggca cggcaagatt gatcgcagtg acaacggacc gcaccgatcg
     1021 ttcgcgagag gacgacatgg aacccgagcc gcgaccgcat gacctcgacc acggatcacg
     1081 cgctccgggt gctcaaaccg ggcagcagcc gtggatcgcg agctggaccc gccgccggtt
     1141 cctcaccggc gcgggcgtgg ccgcggcggg gctcgtcggg ggactcggcg cggcgtgcag
     1201 tggctccggc ggggcttcgg cccgcaagcc agtcccgcgg tcaacccctt cgagcaccgc
     1261 tcccgccaag cccgactcgg ccgggatcgt ggtcggcgat ctgccctcgg aggagcaggt
     1321 tttcggctgg atcaaggagg tggtgagcca cggcatccgc cgtcccggct accccgcgga
     1381 cgtctgggcc gagggttgga ttcaacaaca gttccgcgac atgggcctcg agaacgtgca
     1441 ccgcgaagcg gtcccgctgc gctcctggaa gccgggaacg tcctcgctgc gggcaacgac
     1501 agccacgggt gagacccgcg acatcgagca cttcccagtt ccctacgccg ctccagtgcg
     1561 cgacctcgac atcgagctcg tcgcctacga cgcggcgcac ctgtctgcgg tcgcggcgaa
     1621 ggcgtcgctg tacaccgtga agctcatcac cctgcccgca accgcgctgg ccaggagtgg
     1681 cggcgtgccc aaggacatga ccgggcggat cgtcgacgac gcggagcaat cgctcgccaa
     1741 gggtcagcac acggtgccgt tcgcgcccga gttccaggac gtcgccggtc cttcgatcaa
     1801 ggcaggtgcc gcggcgttca tcgggacgct gaccgcgagc ccgggagaca cctacgagta
     1861 ctacgtgcct tacaccagcg acgagctccc gataccgggc gtgtggatca gcgcaagcag
     1921 cggcaactgg ctccatgatc agttggcgaa ggggccggtg cgcatcgtgc tcaccgtcga
     1981 ctccaccgtc accgaccaca cctcgtacaa catcgtgggc gacctcccgg gtgccgacga
     2041 agaggtcgtg atcgtcgcgt cgcatcacga cgggccgtgg gcgtcggcgg tcgaggacgg
     2101 cagcggcaca tcgctcgtcc tcgcccaggc tcactactgg tcccggcggc cgcgggccga
     2161 gcgaccgcac cggctgcgct tcatcctcca ggccggccac atgtgcggcg gtgcggggat
     2221 gttggcctac atcaaggcgc acggcgagga gcttgggaac gtagtgctgg aagtccacct
     2281 cgaacacgcc gccctcgaag ccgaagagga cagcggcggc aagctcgtca ccactgatcg
     2341 ggcagtgccg cggtggttct tcacgagccg tcacgccacc ctcgaaaaag ccgtctccaa
     2401 cgcgctgacg ggtgagcagc tgacccgatc gatggtgatg gcgcccgatg cgttcggctc
     2461 cgcgcctccc accgacggcc aggggtactt cgccgccggc gttccggccg tcaacttcct
     2521 caccgcaccg tggtacctgt tcgactccgt cgacaccctc gacaagatcg accgcgccca
     2581 cctcgtcccg ctcacccggg ccaccatccg catcatcaag ttcaccgcgg agaccacatc
     2641 cgccaagctc cgcgccgaac caccgacgcc gtgatggctg gtttaccttt cggtcagagt
     2701 cgtggaagag cgtgctgcga cgtgactgag cgcgaggact gttgggtccg gacgtgccgc
     2761 ggcgggcggt gtgtcgacca cgaggcccga cggtgagtcg ttccacgaca aggccgttcc
     2821 cgttacggtc gtgaccgcgg tcgtcggtgt ggcacacacg tcggggagcg tgatcgaacc
     2881 cgtcgcgtct tgcacgaacg cgtacacggt ttcgtctctc gcggtgtacc ggactggttg
     2941 gccgtcgggg gtggtggtgc cgggtgtgac ccagggccgg gttgcggcaa tggctgccgc
     3001 gtgaggtgtg acccattcgg cgagccattc gagtcgggtg agctgttcgt cgggtatccg
     3061 ggcgtcgacc ccccgtgggc cgacgttgag gagcaggttg ccgcccttgg agacgatgtc
     3121 ggtcagcatc cagagcaggt cgtcatgcgc gaggaagtgc tcgggacgtg agcaggcgtt
     3181 gtagccgaag ctctgatcca taccccgcac acactcccaa ggcttgcgtt gcacgtcatc
     3241 gaacacgacg tattcgggcg tgcggacgtc ggaatgcggc gggaccggtg gcacgactcc
     3301 ggcatcgcgc ttggcctggt gtcggacccc ggcatcgatc gcccggcgtc ccaactgtga
     3361 tcgtgccgcc gcgaggagcg ggctccacgg catccaccgg tcgttgacga ctccgtcggg
     3421 gacctgtgcg tagtagtgct cgaagagcgg ccacagctgt ttgccttcgg ccggccacgc
     3481 gatgtcgttc cacagcacgc tcggccggta ccgagcgatg agctcgcgca cctgagcttc
     3541 ggcatacgcg ggatagtcac cgcggggaat cgcgtcgagc atctccgaaa gcgatccgat
     3601 gggccggtcg ttgaacgtcg aatcgagccc acccgagtag tagatcccga agcgcatccc
     3661 ggcaccgcgc accgcctcgc acagttcacc gaccacgtcg cgaccgcagt tccaaccagg
     3721 tcgacgagga ttggggacat tggtcggcca catgcagtag ccatccatgt gcttggttac
     3781 gaacacgacg tacttggcgc cggttgcggc gaaacgcgcc gcccattcgt gcggatccca
     3841 gtgctcgagg ccggctgccc aatcgtcggc gaactgcacg tacgggcggt tgccgtacac
     3901 ctcgcgatga tggcgggcga cgggactgtc gggaaaacgc aaggaattct cgtaccactc
     3961 ggcatacggg caaaacgcga gcgcgtcccg tcggcccgac tgcatcagtt cgccgatatc
     4021 cacgtcgacg ggcgcgaacg ccggcaccga cgccggcgtc cagtgcacga agatccccag
     4081 cttcgcgtcc gaccaccacg cgggcacgcg atgaccgcgc aaagccttga gccgcggatt
     4141 accaaaccgc atcgcccgac ccttctcgag acccccgttc ggccggcgca ccgtagctcg
     4201 cgaaccgtcg ggcgtcgccg cgatcggcgt acgcggcgac agttctggcg cgatgatcgc
     4261 cgagtggaag ccgagcccga gtgtcggacg cgcggaggac atcgaggccg atggcgactt
     4321 cgacgtcggc gcaatcggga acgcgtttca ccgcctccga cgacgccgcg tcgcagagtc
     4381 ggcgcgacgc tggctcaccc cgggtgggca cctcgccctc gtgtggagca catcaccctg
     4441 ggacggagac caggaatggc agcgcgcgcg ctcggatcgc acgcgtccgc gttcgagcaa
     4501 gacgtacgag atcgtctctt gcggattgcg cccgacaacg tcctacgcga ttcgatcggc
     4561 ttccgttaca cccttgcgca tctcctctga gccgcagaac tcgcgggtcg gtgttcctgt
     4621 ggcgcatggt cggtagggtc ctcgcgtctc gtcattttct tggggggcca atggagttcg
     4681 acgtcgtcgt cgtggggggc gggtccgcgg gctgcgt
//
LOCUS       DATCCH010000302         2226 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_199292, whole genome shotgun sequence.
ACCESSION   DATCCH010000302 DATCCH010000000
VERSION     DATCCH010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199292"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="VIJ44_05765"
     CDS             <1..682
                     /locus_tag="VIJ44_05765"
                     /inference="COORDINATES: protein motif:HMM:TIGR01254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA];
                     GO_process: GO:0030974 - thiamine pyrophosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY5165438.1"
                     /translation="RVTPIDHGDVCINYDKAWFSSHQIPVPTSLDDLTKPAYKGRLVV
                     ENPATSSPGLAFVLATIAHYGERGWRAYWSKLRANGVQVVDGWDQAYNGSFTAGGGTG
                     DVPLVVSYASSPPAAVVNASPQPAQSPVGTMLSSCFRQIEFVGVLKTTTHVAAARKLV
                     DFMLSQRFQADMPLQMYVFPVRDGTPLPAVFTKFAQTTSNPLSIPAAQIGRSRDRWIN
                     EWTATVLR"
     gene            679..>2226
                     /locus_tag="VIJ44_05770"
     CDS             679..>2226
                     /locus_tag="VIJ44_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011030361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEY5165439.1"
                     /translation="MTRLPARVGRVTLVAVPLGFLGLFFAYPVGSILARGLAPGGHVD
                     LGPVGQVLTDASLRGIAWFTVWQAALSTLVTVLIALPGAYVVSRFRFPGRGLVRAAIV
                     IPFVLPTVVVGSAFLALGFRPSLGAVVVAHVFFNYAVVVRIVGGFWSHLDPQQDDAAR
                     VLGASRLRAFRTVVLPALRPALLGAAAIVFLFTFTSFGVILILGGPTRSTLETEIYRQ
                     TAELLNLRLAAALSVVQVTAVAAVLLVVGRAEGRHRVALRLRSARDTARRARTGLEQL
                     FVATNLVVMATLLGAPLAVLMARSFATTGGPGFGFYDALGHLHANSVLFVPPVDAIRN
                     SLVFAGEATVIAVGIGGLAAVAVARSRRERFLDGALALPLGVSAVTVGFGFLIALDKA
                     PLDLRTSSLLIPIAQGTVAVPFVVRTIVPVLRSIDPRLREAAMTLGASPARVWREVDG
                     PIVARAGLVAAGFAFAISLGEFGATVFLARPNTQTLPVVIFRLLGQPGELNFGAAMAA
                     STILMALT"
BASE COUNT          262 a          804 c          754 g          406 t
ORIGIN      
        1 tcgggtcacg ccgatcgacc acggcgacgt gtgcatcaac tacgacaagg cgtggttctc
       61 gtcgcatcag atcccggtcc cgacgtcgct cgacgatctg accaaacccg cgtacaaggg
      121 gcgcctggtg gtcgagaacc cggcgacgtc gtcgcccggg ctggcgttcg tcctggccac
      181 gatcgcccat tacggggagc gcggctggcg tgcctattgg tcgaagcttc gagccaacgg
      241 cgtccaggtt gtcgacggat gggaccaggc ctacaacggg tcgttcacgg ccgggggcgg
      301 aaccggcgac gtcccgctcg tcgtgtcgta cgcgtcgagt cccccggcgg cggtcgtcaa
      361 cgccagcccg cagccggccc agtcgcccgt cgggaccatg ctctcgtcgt gcttccgtca
      421 gatcgagttc gtcggcgtgc tcaagacgac cacccacgtc gcggcagccc gcaagctggt
      481 cgacttcatg ctctcgcaac gcttccaggc cgacatgccg ctccagatgt acgtgttccc
      541 cgtgcgtgac ggcaccccgc tgcccgcggt gttcaccaag ttcgcccaga cgacgagcaa
      601 cccgctgtcg atcccggcgg cccagatcgg tcgcagccga gaccggtgga tcaacgagtg
      661 gaccgccacc gtgctgcggt gacccgactc ccagcccggg tcggtcgggt cacgctcgtc
      721 gcggtccccc tcggcttcct cgggctgttc ttcgcctatc cggtcgggtc gatccttgct
      781 cgcggtctcg cacccggagg ccacgtcgat ctcggcccgg tcgggcaggt cctgaccgac
      841 gcgagcttgc gcgggatcgc ctggttcacg gtatggcagg ccgcgctctc gacgctggtg
      901 acggtgctga tcgcgctgcc gggggcgtac gtcgtcagcc ggttccgctt cccggggcgg
      961 gggctggtgc gcgccgcgat cgtgatcccg ttcgtgctcc ccacggtcgt ggtcggatcg
     1021 gcgttcctgg cgctgggctt ccgtccgtcg ctgggcgccg tcgtggtggc gcacgtgttc
     1081 ttcaactacg cggtggtggt gcgcatcgtg gggggtttct ggtcccacct cgacccccag
     1141 caagacgacg cggcccgggt cctcggcgcg agccggcttc gggcgttcag gaccgtggtg
     1201 ctgccggcgc ttcgccccgc gctgctgggc gccgcggcga tcgtcttttt gttcacgttc
     1261 acgtcgtttg gtgtgatcct gatcctcggc ggcccgaccc gatcaaccct cgagaccgag
     1321 atctaccgcc agaccgccga gctcctgaac ctgcgcctcg ccgcggcgct gtcggtcgtg
     1381 caggtgaccg cggtcgccgc ggtcctgctg gtggtgggac gggccgaggg acggcatcgc
     1441 gtggccctcc ggttgcgctc cgcgcgcgat accgcgcgac gggcccggac cggcctcgaa
     1501 cagctcttcg tcgccacgaa cctcgtcgtg atggccacgc tgctcggagc cccactcgcg
     1561 gtgctcatgg cacggtcctt cgccacgacc ggcggccccg ggttcgggtt ctacgacgcg
     1621 cttggccacc tccacgccaa cagcgtcttg ttcgttcccc cggtggacgc gatccgcaac
     1681 tcgctggtgt tcgcaggcga ggcgaccgtg atcgcggtcg ggatcggcgg tctcgccgcg
     1741 gttgcggtgg cccgcagccg gcgggaacgg ttcctcgacg gggcgctggc gttgccgctc
     1801 ggcgtttcgg ccgtgaccgt cgggttcggg ttcctgatcg cgctggacaa ggcaccactc
     1861 gacctgcgta cctcatcgtt gctgatcccg atcgcgcaag gaaccgtcgc ggttccgttc
     1921 gtggtgcgca cgatcgtgcc cgtgctccga tcgatcgatc cccgattgcg ggaggcggcc
     1981 atgacgctcg gcgcatcccc ggctcgggtc tggcgcgagg tcgacggacc gatcgtggct
     2041 cgggccggcc tcgtcgccgc cggcttcgcc ttcgccatct cgctcgggga gttcggcgcc
     2101 accgtgttcc tcgcccgccc gaacacccaa accctccccg tggtcatctt ccggctgctc
     2161 ggccagcccg gtgagctgaa cttcggtgcc gcgatggcgg ccagcacgat cctgatggca
     2221 ctcacc
//
LOCUS       DATCCH010000303         2286 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_116038, whole genome shotgun sequence.
ACCESSION   DATCCH010000303 DATCCH010000000
VERSION     DATCCH010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116038"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="VIJ44_05775"
     CDS             <1..909
                     /locus_tag="VIJ44_05775"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEY5165440.1"
                     /translation="APGLPDRRRRTHLVPLLRGASPSIVIALVTDSNALVPPDLRARF
                     AVDVVPLTVVVDGAPYAEGVDLTTPAFYERLAAGADVSTAAPAPGALLRAYEQAAARG
                     AEAVLSIHIGSNTSATVGSARVAAASSPIPVEIVDTGTASFPVACCVWAAGRVLEHGG
                     DLDEAAAAAQEVSRVVGNVFVVGALDLARRGGRLASGAEDGGGVPVLALRDGTMDVLG
                     RAADIETAVEAMVAEFARASAGELMRVGVGEASAPEVAGELARRLGAQPETAELVRYE
                     VGPSVAAHSGLGTAGAVYFPARLAGP"
     gene            complement(940..1428)
                     /locus_tag="VIJ44_05780"
     CDS             complement(940..1428)
                     /locus_tag="VIJ44_05780"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEY5165441.1"
                     /translation="MPIELLEASRFGFDSRCFVCEPANPRGLQVPFFHDTDADVVFAT
                     FDLPAEFSGAPTLVHGGVTLALIDEAMSWATIAIGHKFAYTGETSARFAWPVRLGRSY
                     RVEARLVDRADRRMVTEAAVLDGKGRPCVTATATMVVLDLDQAADAIGADLGGDDTPY
                     TR"
     gene            complement(1432..>2286)
                     /locus_tag="VIJ44_05785"
     CDS             complement(1432..>2286)
                     /locus_tag="VIJ44_05785"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5165442.1"
                     /translation="EMVQGFTRLGIPTTLLQKGPRILPRDEPALVDLLVGRLRKEGVD
                     LRFNVETEKVSQNGDKKVVHGTEDGKPTTWEADELLVAVGRRPNVDDLGLEELGIETG
                     PKGVIVDNRGRTTVDTVYACGDIAGRFLFTHSAAYEGVRAVRDMFFPGKGKVVASVPW
                     CTFTDPELAHAGMTEAEARDEFKGDVEVWRQDLVHNDRARADDATDGAILVITHKGRI
                     VGAHILAPAAGEMIHEFALAIAEDVKLSELSQFMHVYPTLSTSVGQLAGEAAFEKAEK
                     LRWLVKRK"
BASE COUNT          313 a          834 c          785 g          354 t
ORIGIN      
        1 gctcccggtc tcccagaccg ccgccgccgc actcatcttg taccgcttct acgaggcgcg
       61 tcgccgagca tcgtgatcgc gctcgtcacc gactccaacg cgctcgtgcc gcccgatctg
      121 cgggcccgtt tcgcggtcga cgtcgttccg ctcaccgtcg tcgtcgatgg cgcgccctac
      181 gccgaagggg tcgacctgac caccccggcg ttctacgagc gcctcgcggc cggcgccgac
      241 gtgtcgaccg ccgccccggc accgggcgca ctgctccgcg cttacgagca ggccgcagcg
      301 cgtggcgccg aggccgtcct ctcgatccac atcgggtcga acacgtccgc cacggtcggg
      361 agcgcccgtg tcgcggcagc atcgagcccg atcccagtcg agatcgtgga caccggcacc
      421 gcctcgttcc ccgtcgcgtg ctgcgtctgg gccgcgggcc gcgtgctcga gcacggtggc
      481 gacctggatg aagccgcggc cgcggcgcag gaggtgtccc gcgttgtcgg caacgtgttc
      541 gtcgtcggtg cgctcgacct ggcccgccgg ggcggccgac tggcgagcgg cgccgaggac
      601 ggtggcggcg tccccgtgct cgcgctccgc gacgggacca tggacgtcct cggtcgcgcc
      661 gccgacatcg aaaccgcggt cgaggcgatg gtcgccgagt tcgctcgcgc gtcggccggt
      721 gagctcatgc gggtcggagt cggcgaggcg tccgctcccg aggtcgccgg agagctggcg
      781 cgccgcctcg gggcgcagcc ggagaccgcg gagctggtcc gctacgaggt tgggccgtcg
      841 gtcgccgcgc acagcgggct cggcactgcc ggggcggtgt acttccccgc ccgcctggcc
      901 ggaccctgat caggcagggc gcctgcccgc accgcgggcc tagcgggtgt acggagtgtc
      961 gtctcccccg aggtcggctc cgatggcatc tgcggcttgg tcgaggtcca ggacgaccat
     1021 cgtcgccgtc gccgtcacgc agggccggcc cttgccgtcg agcacggccg cttcggtcac
     1081 catgcggcga tcagcccggt cgacgagccg ggcctcgacc cggtatgacc gccccaggcg
     1141 caccggccac gcgaaccgag ccgaggtctc gccggtgtag gcgaatttgt ggccgatggc
     1201 gatcgtggcc cagctcatcg cctcgtcgat gagcgccagc gtcacccctc cgtgcaccag
     1261 ggtgggcgcc ccggagaact ccgccggcag gtcgaaggtc gcgaacacga cgtccgcgtc
     1321 agtgtcatgg aagaacggga cctgcagccc acgcggattg gcgggctcgc acacgaagca
     1381 gcgactatcg aacccgaacc gtgaagcctc gagcaactcg atgggcacgc gttacttccg
     1441 cttcaccagc caacgcagct tctcggcctt ctcgaacgcc gcctcacccg caagctgacc
     1501 gaccgacgtc gacagcgtcg ggtacacatg catgaactgg ctgagctcgc tcagcttgac
     1561 gtcctctgcg atcgccaacg cgaactcgtg aatcatctct ccggccgccg gcgcaaggat
     1621 gtgcgccccg acgatgcggc ccttgtgggt gatcacgagg atcgccccgt cggtcgcgtc
     1681 gtcagcgcgg gcccggtcgt tgtgcaccag gtcctggcgc cacacctcga cgtcgccttt
     1741 gaactcatcg cgcgcctcgg cctcggtcat cccggcgtgg gcgagctcgg gatcagtgaa
     1801 cgtgcaccag gggacgctgg ccacgacctt gcccttcccg gggaagaaca tgtcccggac
     1861 cgcccggact ccctcgtacg ccgcggagtg ggtgaagagg aaccgccccg cgatgtcacc
     1921 gcacgcgtac acggtgtcga ccgtggtgcg gccgcggttg tcgacgatga cacccttcgg
     1981 gccggtctcg atacccagct cctcgaggcc gaggtcgtcc acgttcgggc gacggcccac
     2041 ggccacgagg agctcgtcgg cttcccacgt cgtcggcttg ccgtcctcgg tgccgtggac
     2101 aaccttcttg tcgccgttct gggacacctt ttcggtctcg acgttgaagc gcaggtcgac
     2161 accctccttg cggagccggc cgacgaggag atcgacgagg gcgggctcgt cgcggggcag
     2221 gatgcgcggg cccttctgaa gcaaggtggt cgggatgccg agacgggtga agccctggac
     2281 catctc
//
LOCUS       DATCCH010000304         4833 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_490177, whole genome shotgun sequence.
ACCESSION   DATCCH010000304 DATCCH010000000
VERSION     DATCCH010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4833
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490177"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VIJ44_05790"
     CDS             complement(<1..291)
                     /locus_tag="VIJ44_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165443.1"
                     /translation="MGRLRWRSLMIVTAIVSATLSSAARVSAAPTPNAAFPAVPVTSC
                     RTVFGAQEAPAPRIPAQLPLAASATTAARLAFYSNGFVTLLGPRGWSCRAVEA"
     gene            complement(331..1308)
                     /locus_tag="VIJ44_05795"
     CDS             complement(331..1308)
                     /locus_tag="VIJ44_05795"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HEY5165444.1"
                     /translation="MRAARCQAYGPPEAVVIEHIPPPTLRAGGALIDIQAAAVNFPDV
                     LLVANEYQVSIPVPFTLGSEFAGVVTAVADDATGLAPGDRVFGSAFAGAFAEQIAMPA
                     AALTRLPDSADFAEAAAFGVAHTTAYLALRSVAEVQADDWVVVLGAAGGVGLAAVELA
                     TILGARVVAAASSADKLALCRARGAVATVNYTTEDLKVRIKDITGGGADVVIDPVGGP
                     YSEPALRATRWGGRFVVIGFAAGEIPRIPLNLVLLKGVVVKGFEFRTFADHAPDAARR
                     DHDELFQLFATGQVRPHVSAVFPLDQTGEALRAMADRRALGKIVVDPTA"
     gene            1637..2074
                     /locus_tag="VIJ44_05800"
     CDS             1637..2074
                     /locus_tag="VIJ44_05800"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY5165445.1"
                     /translation="MARGWLTHLREGAIFKLEDLDDEQLRWKPVATANSLGEIVMHLG
                     YVERLWVRAIFDGETMDMSWRPHMFALPEGWSVEDVVIFYRAETAAADAVLDRAASLD
                     LPSAAEFRPTTLRWVVTHLLEEIARHVGHMDISRELLDGRTGR"
     gene            complement(2119..2469)
                     /locus_tag="VIJ44_05805"
     CDS             complement(2119..2469)
                     /locus_tag="VIJ44_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011891141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165446.1"
                     /translation="MSIPVPLEGLRDEIDRAPASPYLVTVGADGRPHCVSVAVGWAAG
                     LLVADAGNTTVANARARPLVSLVWPPGQPGGHTLIVDATATVGDGSTRRVGLAPSSAV
                     LHRTVSPSGDRVDR"
     gene            2540..4012
                     /locus_tag="VIJ44_05810"
     CDS             2540..4012
                     /locus_tag="VIJ44_05810"
                     /inference="COORDINATES: protein motif:HMM:NF018561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HEY5165447.1"
                     /translation="MTSNRARWRTRSRRIGALIVSLLMVAFASVSATSAATRGTVTPP
                     NDGLQVLRAVAATAAAAAPSGVDRAAYQSAVEAYIWGYPLVAMVRTRATLVCAVGVNT
                     FLDQTTLSGPSSRLVVTPNTDTLYSSAFLDLRAGPVVLRIPALADRYYDFQFLDMSTN
                     TITNVGSRTVGQGPASVAVTESTWKGRLPAGTRRISSPTPDVWIIGRTLPKNDADIPN
                     VAALQHGYALTPVASNGPTPGPDRGQCGRPGPATVSASGAEFFDELGAALAADPPPTS
                     DAPVLRDLSAAGIGVGMRPSTHASPAVIAALGQSIPAANATIAATAARNLTAVNGWSR
                     IQHLGAYGRDYVTRAAVAQTALGANVPAESVYYSAGADRARTPLVGTRPVIVHFAAGQ
                     LPPVDQRGFWSVTLYGPDHFLVANRLDRYAIGDRTTGVQRASDGSLDLYLGAQAPSGH
                     ETNWLPAPAGSYSLVLRAYVPGAAIRQGTWSPPTIGPQIP"
     gene            4429..4833
                     /locus_tag="VIJ44_05815"
                     /pseudo
     CDS             4429..4833
                     /locus_tag="VIJ44_05815"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
BASE COUNT          700 a         1745 c         1634 g          754 t
ORIGIN      
        1 ggcctcgacg gcgcgacacg accacccgcg cggtccgagc agggtcacga agccgttcga
       61 gtagaaggcc agtcgtgccg cggtcgtggc ggaggcggcg agtggaagct gggctgggat
      121 ccgcggcgcg ggcgcctcct gggcaccgaa caccgtccgg caggatgtca ccgggacggc
      181 gggaaacgca gcattcggcg tgggcgcggc cgacacacgc gccgcggacg agagcgtggc
      241 gctgacgatc gcggtcacga tcatgagcga ccgccatcgg agtcgaccca cgaccggcag
      301 cgtatgcatc tgggtcttcg ggcgccggga tcaagcggtc gggtcgacga cgatcttgcc
      361 gagggcccgt cggtccgcca ttgcgcggag tgcctccccg gtctggtcca gggggaacac
      421 cgccgacacg tgaggacgaa cctggccggt cgcgaagagc tggaacagct cgtcgtggtc
      481 tcgtcgtgcg gcatccggcg cgtggtcggc gaaggtgcga aattcgaagc ctttgacgac
      541 cacacccttg agaagcacga ggttgagtgg gatccggggt atctcgcctg cggcgaaccc
      601 gatcaccacg aatcgtcctc cccaacgcgt cgcccggagc gcgggttccg agtacgggcc
      661 acccaccggg tcgatgacga catccgcgcc gccaccggtg atgtccttga tccggacctt
      721 caggtcttcg gtcgtgtagt tcacggtcgc gaccgcgccg cgcgcccggc acaaggccag
      781 cttgtccgcg ctggaggccg ccgccaccac gcgcgctccc aggatggtgg cgagctcgac
      841 ggcggcgagt ccgacgccgc ccgcggcccc gagcaccacg acccagtcgt cggcttggac
      901 ttcggccaca cttcgcaacg cgagatacgc ggtcgtgtgg gcgacgccga acgcggcggc
      961 ctcggcgaag tccgcgctgt ccggtaggcg agtgagcgcc gcggccggca tcgcgatctg
     1021 ctcagcgaac gcgccggcga aggcgctgcc gaacacgcgg tcgcccggtg ccaatcccgt
     1081 cgcgtcgtcg gcaaccgccg tgacgacacc tgcgaactcg cttccgagcg tgaacgggac
     1141 cggaatcgaa acctggtact cgttcgcgac gagcagaacg tcgggaaagt tgacggcggc
     1201 ggcctgaatg tcgatgagcg cgcccccggc gcgcagcgtc ggcgggggaa tgtgttcgat
     1261 cacgaccgcc tcgggcggtc cgtacgcctg gcagcgagcc gctcgcaacg atcgcttccc
     1321 ttcgtgctgg ttgatgatcg tgcccacggg gtgcattctc cgcgagctcg gccgggatgc
     1381 ccacacctta gggaacgcgc cacggttcct ctcggggcgt gttcacgaaa cgccgggact
     1441 gggcggtccg cgagccatta ggtttgacga acttgctgtc ggcggagcgg cgcccgatcg
     1501 tttcccgtct ttcgcaccgg taggcggcga catgtcatcg gagcacgaac ccaacgacac
     1561 gcgtctggct tctgaggccc gccccgagct gcggatcccg aggccggacg aatccgctca
     1621 ggccgacgaa atgaccatgg cgcggggctg gctcacccat ctccgcgaag gcgcgatttt
     1681 caagctcgag gacctcgacg acgagcaact gcgctggaag cccgtcgcga ccgccaactc
     1741 gctcggcgag atcgtcatgc acctgggcta tgtcgaacgg ctctgggtgc gcgccatctt
     1801 cgacggcgag acgatggaca tgtcctggag accgcacatg ttcgccctgc cggagggatg
     1861 gtccgtcgag gacgtcgtca tcttctaccg ggccgagacc gcggccgccg acgccgtcct
     1921 cgaccgggcc gcctcgctcg accttccctc ggcggccgag ttccgtccca ccacgcttcg
     1981 ctgggtcgtg acccacctgc tcgaagagat cgcccggcac gtcggtcaca tggacatcag
     2041 ccgtgagctc ctcgacggca ggaccggacg ctgattaccc gcgggcgccg atcgcgtcgc
     2101 gttcgcgggc ggtcgggttc accgatcgac gcggtcgccg gacggtgaca cggtccggtg
     2161 cagcaccgcg ctggatggag cgaggcccac ccggcgggtc gagccgtcgc ccacggttgc
     2221 ggtcgcgtcg acgatgaggg tgtggccgcc cggctggccc ggtggccaga ccaggctgac
     2281 gagaggtcgg gctctcgcgt tcgcgaccgt cgtgttcccg gcgtcagcga cgagcaggcc
     2341 ggccgcccag ccgacggcga cactgacgca atggggccga ccgtcggccc cgacggtgac
     2401 gaggtacggc gacgccgggg cccggtcgat ctcgtctcga agcccttcca gtggcacggg
     2461 gatgctcata acctgacgct acgccggggc gtctcacctg tgttgaccgg tggtcggctg
     2521 gactctcgga aagggctgca tgacctcgaa ccgtgcccgg tggcgcacgc gctcacggcg
     2581 catcggcgcc ctgatcgtgt cgctgctcat ggtggcgttc gcgtctgtga gtgccacgag
     2641 cgcagcgacc cgcggcacgg tcacgccgcc gaatgacggg cttcaggtgc tgcgcgctgt
     2701 tgccgcgacg gcagccgccg cggcgccgag tggcgtcgac cgtgccgcct accaaagtgc
     2761 cgtggaggcg tacatctggg gctatccgct cgtcgccatg gtccgcaccc gcgccacgct
     2821 ggtctgcgcg gttggagtca acacgttcct cgaccagacc accttgtccg gaccgtccag
     2881 ccggttggtc gtgacaccca acaccgacac gctctactcg agcgcgtttc tcgatctgcg
     2941 cgccggacca gtcgtactcc ggatccctgc cctcgccgac cgctactacg acttccagtt
     3001 cctcgacatg tccaccaaca ccatcaccaa cgtcggatcc cggacggtcg gtcaggggcc
     3061 cgcctcggtt gcagtcacgg agtccacgtg gaagggacgg ctcccagccg gcacccgccg
     3121 gatcagttca ccgacacccg acgtgtggat catcggacga accctcccga agaacgatgc
     3181 cgacatcccg aacgtcgccg cgctccagca tggatacgcc ctgacgcctg tcgcgtcgaa
     3241 cggccccacg cccggaccgg atcgcggcca atgcggacgg ccgggtccgg caacggtgag
     3301 cgcttccgga gcggagttct tcgacgagct cggtgccgcg ctggccgcgg acccgccccc
     3361 gaccagcgac gcgccggtgc tgcgcgatct cagcgccgcc gggatcgggg ttgggatgcg
     3421 cccgtcgacg cacgcgagcc cggcggtcat cgcggccttg ggccagagca tccccgccgc
     3481 gaacgccacg atcgccgcca ccgcagctcg gaacctcacg gcggtcaatg gctggtcgcg
     3541 gatccaacac ctcggggcct acggccgcga ctacgtgaca cgcgccgccg tcgcccaaac
     3601 cgcgttgggt gcgaacgttc ccgccgagtc cgtctactac tccgcgggag ccgaccgcgc
     3661 ccggacaccg ctcgtcggca cacggccggt gatcgtccat ttcgccgccg gtcagctgcc
     3721 gccggtcgat cagcgcgggt tctggtccgt caccctctac ggacccgacc acttcctggt
     3781 cgccaaccgg cttgaccggt acgcgatcgg cgaccggacc acgggcgtcc agcgggcctc
     3841 cgacggatcc ctcgacctct atctgggcgc ccaggccccg tccggtcacg agaccaactg
     3901 gctcccggcg ccggccggca gctacagcct ggtgctgagg gcctacgtcc cgggcgccgc
     3961 catccgccag ggcacctgga gccccccgac gattggaccc cagattcctt gagtcgaccc
     4021 ggctgaacga ctccccggga ccgaatcgcg ttcaggtttg tggccccgga tgccgatccg
     4081 gtcgtgtgcg agcccgcacc tcgtccgtcg ccgggcgggc gccgatcggt gagctgcccg
     4141 gtccccacct cgtcgcgcgt gaacccgacc gctgtccggt gaggcccggt tgacggtcgc
     4201 tctcgccccg gctcgcgacg accgcctgct tggctcgtcg ccggtgctgc gccgatggga
     4261 gcgtggcctg ctccagatcg tcacggtgtc gtgggtgtac gccatctatg acgagctgcg
     4321 ggccgccgca ccggtctcgg catcgagcgc gccgtccagg aggccacgtt ccatctcccc
     4381 tggctcgtgg gcctctgcaa tgtctgctag agcgcgacgc acctcgtcgt cccacccatc
     4441 gtgctcgtgg ccctctaccg gacggcgccc gaccggtacc gacagtggcg cgacgtcttt
     4501 ctcgtcatgc tcgggctctc gctgttgtgc ttctggctgt atcccgtgct gccaccgcgg
     4561 ctggtgccgg gcgcccatca cgtcgctgac acctccagct cctacttcaa tgtcgaccag
     4621 atccccggtg ccgggatcgt cacggccgcg acgcgctcga gcgctccgca atgggcgccg
     4681 ttcacgaacc cgatggccgc gatgccgagc ctccacgtcg cctgggcggt ctgggcttcg
     4741 ctcgcgatct ggcctgttgt gcggcgacgc tggagtgggc cgtgggtggg cggtcgcgtc
     4801 gtaccccctg gcgatgacct ggtcggttct cgt
//
LOCUS       DATCCH010000305         4258 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_323039, whole genome shotgun sequence.
ACCESSION   DATCCH010000305 DATCCH010000000
VERSION     DATCCH010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4258
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323039"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="VIJ44_05820"
     CDS             <1..317
                     /locus_tag="VIJ44_05820"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEY5165448.1"
                     /translation="LRIVPVTDVDAAELVRSLRGSPMLFGYRNLAPVAVDVLEDLLVR
                     VGQLADAVPEVAELDCNPVIVSPAGATAVDVKVRLAPVGPVPPPGVRRLREPEKPAES
                     GT"
     gene            335..574
                     /locus_tag="VIJ44_05825"
     CDS             335..574
                     /locus_tag="VIJ44_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165449.1"
                     /translation="MGGFDLSDRPIEDGTEVARVRFHRHPKMEVNSTISMNVLIEKAR
                     ETPRLEWGALDAMRDSVDNAVRVLTPFVVRPPATA"
     gene            680..928
                     /locus_tag="VIJ44_05830"
     CDS             680..928
                     /locus_tag="VIJ44_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165450.1"
                     /translation="MRVTRKILLVSLATSVVLFVVAAPFGNVKNHSSFGYQFGNVVFA
                     MFLISVPVFLVLLIIAVVQAVMARRRTSAEPSAVSGLG"
     gene            complement(1014..>4258)
                     /locus_tag="VIJ44_05835"
     CDS             complement(1014..>4258)
                     /locus_tag="VIJ44_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014688710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HEY5165451.1"
                     /translation="TQVRYAELHCHTNFSFLDGASHPEELVDEAARLGLAALAVTDHD
                     GFYGIVRFAEAARPVGLPTVFGAELTLGAPRPANGVADPSGEHLVVLAHGPAGHARLA
                     RAISAGQLAGEKGAPSFTLDGLADAARVGVHLTGGRPDRSNESWWVLTGCRKGTVPRA
                     LLADGPAVARRSLDRLVDAFGRSRVLVELWDHGDPIDRHRNDALAEVAARAGVEVIAT
                     NNVHYATSAQRPLATALAAIRARRSLDEIDGWLPASPLARLRSPAEQARRFARWPGAV
                     ERTVEVAQACAFDLRLAAPDLPDLDVPSGHTDMSWLRVLTARGAAVRYPATHPCYEQA
                     MRQLAYELDVIDTLNFPGYFLLLVDIVQFCATHDIYCQGRGSAANSAVCYALGVTKAD
                     AVALGLLFERFLSPERDGPPDIDLDIEHQRREEVIQYVYTKYGRDRAAQVANVITYRP
                     RSALREMAKAVGLSTGHADALTKWIDRWRSRDQAFADVTEAEGAPPVPELVLDLARQV
                     LDYPRHLGIHSGGMVMADRPLVEFCPVEWARMDNRSVLQWDKDDCAAAGLVKFDLLGL
                     GMLTMLHLAVELIHEHEDTDVDLATIPQEDEVYDLLCAADTIGVFQVESRAQMATLPR
                     LRPRTFYDLVIEVALIRPGPIQGGSVHPYLRRRSGEEPVTYPHPVLEPCLRKTLGVPL
                     FQEQLMQIAIDAAGFTPGEADRLRQAMGSKRSRTRMAAMRDRLMEGMAERGITGETAE
                     EIAHKLEAFADFGFPESHSVSFAYLVYASAWMKRHYPAPFACALLNAQPMGFYSPHTI
                     VRDAIRHGVQVLSPCVHASRRDCTLEARTPDAGPVGFPRPGWCADVSTHAVRVGLRYV
                     RGLSDRLLDRIDDARREHPFTDLEDFARRTEAPVDALEALATAGAFAGFGVARREGLW
                     AAGALRDARPGTLPGIVTGIEAPPLPGMSEMEATAADLGATGLSTARHPTEFVRDELT
                     AQGVVTAEELRTRRHRARVEIAGVVTHRQQPETAKGVVFLNLEDETGLVNVICTPDVW
                     QRFRRVARTSPALRIRGRLEREQGVINLVAEHIATLALHPAALPRSRDFH"
BASE COUNT          676 a         1344 c         1565 g          673 t
ORIGIN      
        1 cgctgcgcat cgtgccggtc accgatgtgg acgccgcgga gctcgtgcgg tcgctgcgag
       61 gttccccgat gctgttcgga tatcgcaatc tggctcccgt cgcggtcgac gtgctcgagg
      121 atcttctggt ccgggtgggt cagctcgccg acgcggtccc ggaggtggca gagcttgact
      181 gcaatccggt gatcgtgtcg ccggccggtg caacggcggt ggacgtcaag gttcggctcg
      241 caccggtcgg gccggtcccc ccgccgggag tgcggcgact gcgggagccg gagaagccag
      301 cggagtcagg gacctaggag ggtttggcga aacgatgggc ggcttcgatc tgtccgaccg
      361 gccaatagag gacggcacag aggttgcacg cgtccgcttc catcgacacc cgaagatgga
      421 ggtgaattcg acgatctcca tgaacgtact catcgagaaa gccagagaga cgccacgctt
      481 ggaatggggc gccctcgatg ccatgcgaga cagcgtcgac aacgcggtcc gggttctcac
      541 gcctttcgtt gttaggccgc cagccaccgc gtagcccgct caccgcgcac cccccacggg
      601 ccaaagcgtc gacgaaacag cgcgagccgg gagcccgtat gctccaggcc gaccatggca
      661 acggggaagg ggccgaacca tgagagtcac ccgcaagatc ctgctcgtct ctctggcgac
      721 atcggtggtg ctgttcgtag tcgccgcgcc gttcgggaac gtgaaaaatc acagcagttt
      781 cggttaccag ttcggcaacg tcgttttcgc gatgttcctg atcagcgtgc cggtgtttct
      841 tgtcttgttg attatcgccg tagtccaggc ggtcatggcc cggcgcagga ccagcgctga
      901 gccatccgcg gttagcgggc tggggtaacc ccacccgtga tctgaggtag cggcccgacc
      961 acaaccgccc cgatacggtg cggcgctatg gccgcgactt tcgccaaacc ctcctagtgg
     1021 aagtcccgcg atcgcgggag cgcggcggga tgcaacgcga gggtcgcgat gtgctcggcg
     1081 acgaggttga tcaccccttg ttcgcgttct aagcgtccgc ggatgcgcag ggcgggcgac
     1141 gtgcgggcca cgcgccggaa gcgctgccac acgtcggggg tgcagatcac gttcacgagg
     1201 ccggtctcat cctcaaggtt gaggaagacg acacccttgg cggtttcggg ctgctggcgg
     1261 tgcgtgacca cgccggcgat ttcgacccgg gcccgatgcc gtcgggttcg cagctcctcg
     1321 gcggtgacga caccttgggc ggtgagttcg tcgcgaacga actcggtggg gtgacgggcc
     1381 gtcgagagtc cggtggcacc gaggtcggcg gcggtcgcct ccatctcgga catcccgggg
     1441 agtgggggag cttcgatgcc ggtcacgatg cccgggaggg tgcccggccg ggcatcgcgt
     1501 agcgctcccg cggcccagag cccttcgcgg cgggccacac cgaacccggc gaacgccccg
     1561 gcggtggcga gagcctcgag ggcgtcgacg ggggcttcgg tgcgtcgggc gaaatcctcg
     1621 agatcggtga atgggtgttc gcggcgtgcg tcgtcgatcc ggtccaagag ccggtccgag
     1681 agtccgcgga cgtagcgcag tcctacccga actgcgtggg tggacacgtc tgcgcaccaa
     1741 ccgggtcggg ggaagccgac tggtcccgcg tcgggggttc gggcctcgag ggtgcagtcg
     1801 cgtcgtgagg cgtgaacgca gggactgagg acctggacgc cgtggcggat ggcgtcgcgc
     1861 acgatggtgt gcggtgagta gaagcccatg ggctgggcgt tcagcagcgc gcaggcgaac
     1921 ggcgccgggt agtgccgttt catccaggcg ctggcgtaga cgaggtacgc gaagctcacc
     1981 gaatgacttt cggggaaccc gaagtcggcg aacgcttcca gcttgtgggc gatctcctcg
     2041 gcggtctcac cggtgattcc gcgctcggcc attccttcca tgagacggtc ccgcatcgcg
     2101 gccatccgcg tccgtgagcg tttcgaaccc atggcttgac gcaaccgatc ggcttctccc
     2161 ggggtgaacc cggccgcgtc gatggcgatc tgcatgagct gctcttggaa gaggggcacg
     2221 ccgagggtct tgcggaggca gggttcgagg accggatggg gataggtgac gggctcttcg
     2281 ccgctgcggc gacgcaggta ggggtggacg gagccaccct ggatcggccc gggccggatc
     2341 aaggccacct cgataaccag gtcatagaac gtgcggggcc ggagacgagg caaggtggcc
     2401 atctgggctc gactctcgac ttggaacaca ccgatggtgt cggcggcgca cagcaagtcg
     2461 tagacctcgt cctcttgagg aatcgtggcg aggtcgacgt cggtgtcctc gtgctcatgg
     2521 atcagctcga cggcgaggtg cagcatcgtg agcatcccga ggccgagcag gtcgaacttc
     2581 accaggccgg ccgcggcgca gtcatccttg tcccactgga gcaccgagcg gttgtccatg
     2641 cgggcccact cgaccgggca gaactccacg aggggccggt cggccatcac catcccgccg
     2701 gagtggatcc caagatgacg cgggtagtcg agcacctgac gggcgaggtc gagcacaagc
     2761 tcagggacgg gtggggcacc ttcggcctcg gtgacgtcgg cgaaggcctg gtcgcgggat
     2821 cgccaccggt cgatccactt ggtgagcgcg tcggcgtgcc cggtcgagag gccgacggct
     2881 ttcgccatct cccgcagcgc tgagcgcggc cggtaggtga tgacgttcgc cacctgcgcc
     2941 gcgcggtcgc gcccgtactt cgtgtacacg tactggatga cttcctcgcg gcgttgatgc
     3001 tcgatgtcga gatcgatgtc cggtgggccg tcgcgttctg gggagaggaa ccgctcgaag
     3061 agcaacccga gggcaaccgc gtcggctttg gtgacgccga gcgcgtagca gacagcgctg
     3121 ttggcggcac tcccccgccc ttggcagtag atgtcatgcg tggcgcagaa ctggacgatg
     3181 tcgaccagca gcaagaagta ccccgggaag ttcagggtgt cgatgacatc gagctcgtac
     3241 gcgagctggc gcatcgcctg ctcgtagcaa ggatgagtcg ccggatagcg caccgcggcg
     3301 ccacgcgcgg tgagcacgcg aagccaggac atgtcggtgt gacctgatgg cacgtcaagg
     3361 tcgggaaggt cgggggcggc cagccgcaga tcgaaggcgc acgcctgggc gacctcgacg
     3421 gtgcgttcca ccgccccggg ccaacgggcg aaacgtcggg cctgttccgc cgggctccgc
     3481 agccgggcga ggggggaggc gggcagccag ccgtcgatct cgtcaaggga gcggcgcgcc
     3541 cggatcgcgg ccagcgcggt ggcgagtggg cgctgcgcgc tcgtcgcgta gtggacgttg
     3601 ttggtcgcga tgacctcgac cccggcgcgg gcggcaacct cggccaaggc gtcgttgcgg
     3661 tgacggtcga tcgggtcgcc gtgatcccac agctcgacaa ggactcggct ccgtccgaac
     3721 gcgtcgacga ggcgatccaa cgatcggcgc gccacggcgg gcccgtcggc gagcaacgct
     3781 cggggcacgg tccccttgcg acagcccgtg agcacccacc aggactcgtt cgaccggtcg
     3841 ggccgaccac cggtgaggtg gacgccgacc cgggcggcgt cggcgagccc gtcgagcgtg
     3901 aacgacggtg cgcccttttc tccggccagc tgaccggcgc tgatcgctcg ggccaagcgg
     3961 gcgtgtccgg ccggtccgtg cgccagcacc acgagatgct cgccggaagg gtcggcgacc
     4021 ccgttggcgg gccggggggc cccgagggtg agctcggcgc cgaagacggt gggcaatccg
     4081 accgggcgcg ccgcctcggc gaaacggacg atgccgtaga agccgtcgtg gtcggtgacc
     4141 gcgagggccg cgagcccgag acgggcggcc tcgtccacca gctcctcggg gtggctcgcc
     4201 ccgtccagga aggagaaatt ggtgtgacag tgcagctccg cgtaacgcac ctgggtct
//
LOCUS       DATCCH010000306         4749 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_282586, whole genome shotgun sequence.
ACCESSION   DATCCH010000306 DATCCH010000000
VERSION     DATCCH010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4749
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282586"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1160)
                     /locus_tag="VIJ44_05840"
     CDS             complement(<1..1160)
                     /locus_tag="VIJ44_05840"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014142167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY5165452.1"
                     /translation="MPGPDEPLRVAYLVYRGNPHCGGQGVYTRELVRELTDLGHDVTV
                     FSGPPYPDLADPGRLVRVPSLDLYRPSNPFRVPWPWELRTSVDVREFAIMCGAGFPEP
                     YTFSLRAQRLLAGRRADFDLVHDNQCLGRGLLTMMERDHWPVLATLHHPITVDRDLDL
                     AHARGPWRRITLRRWYGFLEMQMAVARRIPRLVTVSESSQSDIVEQMGVPAERLHVVP
                     VGVDPAVFHPLPSVTRVPGRLMTTASADVPMKGLVHLLEALAKVRTERDDAHLVVIGR
                     PKPKSKIPALIDRLGLGGTVEFVSGVTTERIVELYAEAEVACVPSLYVGFSLPAVEAM
                     ACGVPVVGTTGGAVPEVIGRDNETGLLVPPGDPSALAAAMLRALGDEALRARV"
     gene            1199..3007
                     /locus_tag="VIJ44_05845"
     CDS             1199..3007
                     /locus_tag="VIJ44_05845"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEY5165453.1"
                     /translation="MGDPISAAMIAWSRVVAEPRWSPDGSRLAWVAAFGGRSDVVVAP
                     GDGSGPAVVATGDTGVTRVGAYGGGGFAWVGSDRLVVADTDGRLVTVPGDGGPAALVG
                     ADGRAAAPSPSPDGRQVAFILEDDDRCVIAVAPIDGSSPPVVLSAADYSWDPAWSPDG
                     RSVAWHEWDLPNMPWDESRIVLAAPDGRDRRVIAGDAGEAVGQPRWRADGMLAYVTDR
                     DGCWNVWVGTTEDPGAHPVLPEPCEQAEAPWGPGQRSFAFSPDGQLVAMTRNEDGFGR
                     LTVVGADGARAVARGWHHGLDWGPAGIVAVRSGARTPSAVTVTDVDGARRVVARGPVG
                     GFEGAGLVEPEPTTWIGDDGVTVHGLRWLPSRSALGPGTRPPLLVDCHGGPTGQSTAG
                     WRPWVQYFVTRGWAVLAPNPRGSTGYGRAYAQALSGEWGHLDVADVAAGIRDAAAHGW
                     CDPHRVAVNGGSAGAMTALLLCAHHPDLVHAGISMYGVTDLVELAETTHRFESRYTDR
                     LVGVLPRDADRYRDRSPVTHAASIRVPLLVLQGDADRVVPPEQAQRLVDAVRRAGGHV
                     DAHVYPGEGHGWSRPETVVDVYQRTEAFLRRTVLGR"
     gene            3054..3698
                     /locus_tag="VIJ44_05850"
     CDS             3054..3698
                     /locus_tag="VIJ44_05850"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEY5165454.1"
                     /translation="MSAYAGPRTGADRAVLLAHGAGSDHRAPALVAVADALAAAGIPS
                     LRFDYPYRAAGRRAPDRPPVLAQGTREAASELARRSGLPLERLVLGGRSMGGRIASVV
                     VADEADPQPALGLLLLGYPLHPAGKPERRRDGHFVRLDLPVCFVSGTRDSLAPRSELT
                     RAARKVPGPATVHWLESADHGYRPLTASGRSIDDVLVEAAETSVTWVQGLAESR"
     gene            3784..4266
                     /locus_tag="VIJ44_05855"
     CDS             3784..4266
                     /locus_tag="VIJ44_05855"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY5165455.1"
                     /translation="MRVRRTFAFVDLCGFTRFTDTHGDEQAVDVLTRFRAAVREIGSN
                     HGVRVAKWLGDGAMFVSTSEEPLVESILALAHRCDELGVTLPLRGGLAGGAVILFEGD
                     DYIGGPVNLAARLCDAAGPRELLATADLADAVPAWAAAVPVGELVIKGFVLRVDVVRI
                     "
     gene            4320..>4749
                     /locus_tag="VIJ44_05860"
     CDS             4320..>4749
                     /locus_tag="VIJ44_05860"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY5165456.1"
                     /translation="MAEPTYETLRFDRHGTVGWLHLNRPEKLNAFTPQMWRELGELGG
                     KLRDDPNLRALVVIGEGRAFSSGIDTSVFSDPQPADSVLSGGDARHPDPTVSGILAVQ
                     DSYTWLEEAPYVTIAAVHGYAFGAGLQLALACDIRVVARGT"
BASE COUNT          621 a         1690 c         1748 g          690 t
ORIGIN      
        1 acgcgggcgc gcagcgcctc gtcgcccagt gctcgcagca tcgcggcggc gagtgccgac
       61 gggtcacccg gcgggaccag gagtccggtc tcgttgtccc tcccgatcac ttcgggcacc
      121 gctcctccgg tggtgccaac caccgggacg ccgcaggcca tcgcctcgac cgcggggagc
      181 gagaatccta cgtagagcga cgggacgcac gccacctcgg cttccgcgta gagctcgacg
      241 atccgttcgg tggtgacgcc gctcacgaac tccaccgtgc cgccgagccc gagccggtcg
      301 atgagagcgg ggatcttgct cttgggcttg gggcgaccga tgacgacgag gtgcgcgtcg
      361 tcacgctcgg tgcggacctt ggcgagggcc tccaagaggt ggaccaggcc tttcatcggc
      421 acgtcggcgc tcgccgtggt catgagccgg cccgggaccc gggtcaccga cgggagcggg
      481 tggaacacgg ccgggtcgac gccgacgggg acgacgtgga ggcgctcggc ggggacgccc
      541 atctgctcga cgatgtcgct ctgcgacgat tcggacaccg tcaccaggcg ggggatgcga
      601 cgggccacgg ccatctgcat ctccaggaac ccgtaccagc ggcgcaaggt gatgcggcgc
      661 cacggaccac gggcgtgcgc gaggtcgagg tcccggtcca cggtgatggg gtggtgcagg
      721 gtggcgagca ccggccagtg gtcgcgttcc atcatggtga gcagcccgcg gccgaggcac
      781 tggttgtcgt ggacgaggtc gaagtcggcg cggcgcccgg cgagcaggcg ctgggcccgc
      841 aggctgaagg tgtacggctc cgggaatccg gccccgcaca tgatggcgaa ctcgcggacg
      901 tcgaccgagg tgcgcagctc ccacggccac ggcacccgga acgggttcga gggccgatac
      961 aggtcaaggc tggggacccg gacgagccgg cccgggtcgg cgaggtcagg gtacgggggc
     1021 cccgagaaga ccgtcacgtc gtggcccagg tcggtgagct ctcggaccag ctcgcgggtg
     1081 tagacccctt gaccgccgca atgggggttg ccccggtaga ccaggtacgc gacgcgaagt
     1141 ggttcgtcgg gacccggcat gaggccgaga gcctatcggt cgcatacggt gactctcggt
     1201 gggcgacccg atctcggcag cgatgatcgc ctggtcgcga gtcgtcgccg agcctcgctg
     1261 gtcaccggac ggcagccggc tggcctgggt cgcggcgttc gggggtcgca gcgacgtcgt
     1321 cgtcgccccg ggcgacggat ccggtcccgc ggtggtcgcg acgggcgaca ccggggtcac
     1381 ccgggtcggt gcctacggcg gtggtgggtt cgcctgggtc ggctcggatc gtctcgtcgt
     1441 cgccgacacc gacgggcgcc tcgtgacggt gcccggggac gggggtcccg ccgcgctggt
     1501 gggggccgac ggccgcgccg cggcaccgtc ccccagcccc gacggccgtc aggtggcgtt
     1561 catcctcgag gacgacgacc ggtgcgtaat cgccgtggcc ccgatcgacg ggtcgtcgcc
     1621 cccggtggtg ctgtcggcgg cggactactc ctgggatccg gcctggtcgc ccgacggcag
     1681 aagcgtggcg tggcacgagt gggacctgcc gaacatgccg tgggacgaat cccgcatcgt
     1741 cctggccgcc ccggacggcc gggaccggcg ggtcatcgcg ggcgacgctg gagaagccgt
     1801 cgggcagccc cgctggcggg ccgacgggat gctggcctac gtcaccgacc gcgacggctg
     1861 ctggaacgtc tgggtcggca ccaccgagga tcccggcgcc cacccggtcc tcccggagcc
     1921 gtgcgagcag gccgaggccc cgtgggggcc gggccagcgg tccttcgcgt tctcacccga
     1981 cgggcagctc gtcgcgatga cccgcaacga ggacggcttc ggtcgcctca cggtggttgg
     2041 cgcggacggc gcccgcgcgg tggcgcgcgg ctggcaccac ggcctcgatt gggggccggc
     2101 gggcatcgtc gcggtccggt cgggggcgcg gacgccgagc gcggtcacgg tcaccgatgt
     2161 ggacggcgcc cgacgtgtcg tggctcgcgg tccagtcggt ggcttcgagg gtgccgggct
     2221 cgtcgagccc gaaccgacca cctggattgg tgacgacggg gtcaccgtcc acgggctccg
     2281 ctggctgccg tcgcggtcgg cgctcggtcc cgggacccga ccaccattgc tcgtcgattg
     2341 tcacggtggt cccaccggtc aatccaccgc gggttggagg ccctgggtgc agtacttcgt
     2401 cacccggggc tgggcggtgc tggcaccgaa cccgcgcggc tcgaccggat acggccgcgc
     2461 ctacgcgcaa gccctgtcgg gggagtgggg tcatctcgac gtcgcggacg tcgcggccgg
     2521 gatccgcgac gccgccgcac acggatggtg cgacccacac cgagtcgcgg tcaacggagg
     2581 cagcgccggc gcgatgacgg cgctgttgct ctgcgcccac cacccagacc tcgtgcacgc
     2641 ggggatcagc atgtacggcg tcaccgatct cgtcgagctc gccgagacga cccaccgctt
     2701 cgaatcgcga tacaccgacc ggctggtcgg cgtgttgccc cgcgacgcgg accggtaccg
     2761 cgaccgttca cccgtcacgc acgcggcatc gatccgggtt cccctcctcg tcctccaggg
     2821 cgacgccgat cgcgtcgtgc cgccggaaca ggcgcagcgc ctcgtcgacg cggtgcgccg
     2881 cgccggcggg cacgtcgacg cgcacgtcta ccccggtgaa gggcacggct ggtcccgccc
     2941 cgagaccgtc gtcgacgtct accagcgcac cgaggcgttc cttcggcgca cggtcctcgg
     3001 gcgatgaccc gtccttccca cgtcatatcg gtggcgaccg tcacggggcg gtcatgagcg
     3061 cgtacgccgg accgcgaacc ggtgccgacc gggccgtgct cctcgcccac ggcgcggggt
     3121 ctgaccatcg ggccccggcg ttggtcgctg tggccgacgc gctggccgcg gccgggatcc
     3181 cgtcgctgcg cttcgactac ccctaccggg ccgcgggccg ccgggccccc gaccggcccc
     3241 cggtcctcgc ccagggcacc cgcgaggcgg cgtcggagct ggcccgccgc agtgggctgc
     3301 ccttggagcg cctggtgctg gggggccgct cgatgggtgg gcggatcgcg tcggtggtcg
     3361 tcgccgacga ggcagatccg cagcccgcac tggggttgct gttgctcggc taccccctcc
     3421 acccggcggg gaagcccgaa cggcgccgag acggccactt cgtccgactc gaccttcctg
     3481 tgtgctttgt gagcggcacc cgagattcgc ttgccccccg ctctgagctc acccgggcgg
     3541 ctcggaaggt gccgggtccg gcgaccgtcc actggctcga gtcggcggac cacggctacc
     3601 ggccgctcac ggccagcggc cgctcgatcg acgacgtgct cgtcgaggcc gccgagactt
     3661 cagtcacatg ggtgcaaggt ctcgccgaaa gccgctaatc ggcgccgcga agaccacgta
     3721 aatggttcgt taatcgacga gaattgtacg agatcgccgg ggcgcgcctg cgacactgga
     3781 cccatgcgag ttcggcgcac cttcgctttc gtggacctgt gcggcttcac tcggttcacc
     3841 gacacccacg gtgacgagca ggccgtcgac gtcctgacgc gctttcgcgc cgcggtgcgc
     3901 gagatcggct cgaaccacgg ggtgcgcgtc gcgaaatggc tcggtgatgg cgccatgttc
     3961 gtcagcacgt ctgaggaacc cctcgtcgaa tcgatcctcg ctctggccca ccgctgcgac
     4021 gaactcggtg tcacgcttcc gctgcgaggc gggctcgctg ggggcgcggt gatcctcttc
     4081 gagggcgacg actacatcgg aggcccagtc aaccttgcgg cgcggctgtg cgacgccgcg
     4141 gggcctcgag agctgctcgc caccgccgat ctcgccgacg ccgtgcccgc ctgggctgcg
     4201 gcggtcccgg tcggcgagct cgtgatcaag ggcttcgtgc tgcgggtcga cgtggtgcgc
     4261 atctagcacc tcgcggtgtc cgtcccggtt gccgggggcg gccgatagcc tcagcgcccg
     4321 tggccgaacc gacctacgag acgctgcgct tcgaccgcca cggcaccgtc ggctggctgc
     4381 acctcaaccg gcccgagaag ctcaacgcgt tcacaccgca gatgtggcgc gagctcggcg
     4441 agctcggcgg gaagctgcgc gacgacccga acttgcgggc gctggtcgtg atcggcgagg
     4501 gccgcgcgtt ttccagcggg atcgacacgt cggtgttcag cgacccccag cccgctgatt
     4561 ccgtgctcag cggcggcgac gcgcgccacc ccgacccgac cgtttccggg atcctcgcgg
     4621 tgcaggactc ctacacctgg ctcgaggaag ccccgtacgt gacgatcgcc gccgtgcacg
     4681 gctacgcgtt cggcgccggc ctgcagctcg cgctcgcctg cgacatccgg gtcgtggctc
     4741 gcggtaccc
//
LOCUS       DATCCH010000307         2558 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_223989, whole genome shotgun sequence.
ACCESSION   DATCCH010000307 DATCCH010000000
VERSION     DATCCH010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223989"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="VIJ44_05865"
     CDS             complement(<1..594)
                     /locus_tag="VIJ44_05865"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015794568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEY5165457.1"
                     /translation="MTEISLDGKTALVTGASRGIGAAIAVIYAAAGANVVLSSRKADA
                     LDEVAATLPSERVMTFAANAGEPDQAAACVEATVERFGALDILVNNAATNPYMGPSID
                     IDLGRYDKTFQVNLRGPLVWTQAAWQASMRDRGGVVINVASIGGIRGEAGIGIYNTTK
                     AGLIHLTETLAADLAPGVRVNALAPGLVKTHFARALWE"
     gene            complement(650..1819)
                     /locus_tag="VIJ44_05870"
     CDS             complement(650..1819)
                     /locus_tag="VIJ44_05870"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEY5165458.1"
                     /translation="MTRLAVNDEELVTCVACGLCLPHCPTYRVTGDESASPRGRIAAM
                     RAVHSGTAPLDTEFTRYMTECVQCRACEAACPSSVAFGHLMEGARETLAAETSTVPWW
                     QRLGYSVLEHHRLLVGLSRLLALAQRARLVPRRLGVPHLPLRSSPLTATGHDVWLFPG
                     CVMDAWQREVHAAVMRVLRAAGVDVAIPDPRRAPCCGALHVHAGLHDRAQALARRVVE
                     AFPGDAPILVDSAGCGAALQDYGHLLGTDEARAFSRRVVDVHQWLAARLDRLPATVAG
                     PVPPVVAVHDPCHLRHVQKAHLAVRAILSRYVPVVELDDDGLCCGAGGAYAALQPELG
                     TAIRERKLHAIERTGASVVASANPGCTWFLAAAGVDIRHPLEIVDAALTARPRRP"
     gene            1981..2418
                     /locus_tag="VIJ44_05875"
     CDS             1981..2418
                     /locus_tag="VIJ44_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165459.1"
                     /translation="MADGTQARLEGGGAALLGDHRRLAVVTARLRVGAGVAFLLTAGP
                     LARVTLGSTSREARAAMRLTGARDLALGLGALTTVKERTQDAEWVSMGALVDGLDAVV
                     LLGTRRLPLRARLIGVIAAITAVTGLWVSQRLADERTDPDRST"
BASE COUNT          359 a          827 c         1012 g          360 t
ORIGIN      
        1 ttcccagagc gcacgggcga agtgcgtctt gaccagaccg ggggcgaggg cgttgacacg
       61 cacaccgggg gcaaggtcgg cggcgagggt ctcggtcaga tggatgagac cagccttggt
      121 cgtgttgtag atgccgattc ccgcctcgcc ccggatgccg ccgatcgatg cgacgttgat
      181 gacgacgccg ccgcggtcgc gcatcgaggc ttgccacgcg gcttgggtcc agaccaatgg
      241 tccccgcagg ttgacctgga acgtcttgtc gtagcgaccg aggtcgatgt cgatgctggg
      301 ccccatgtag ggattcgtcg ctgcgttgtt gacaaggatg tcgagggcgc cgaaccgttc
      361 gaccgtggcc tcgacgcaag ccgcggcttg gtcgggctct ccggcgttgg cggcgaaggt
      421 catgacgcgt tcactcggaa gcgtggccgc gacctcgtcg agcgcatcgg ctttccggga
      481 ggacaacacg acgttggcgc ccgcggcggc gtagatgacg gcgatcgcgg cgccgatccc
      541 acgggaggcg ccggtgacga gggcggtctt gccgtcgagg ctgatctcgg tcatcgggac
      601 ctccgaggcg ggtgctgtcg gcgggtgcgg cgggtcgggg tgcggtgcat catggtcgac
      661 gggggcgtgc ggtgagcgcg gcgtcgacga tctcgagcgg atgacggatg tcgacgcccg
      721 cggcggcgag gaaccaggtg cagcccggat ttgcgctcgc cacgaccgaa gcgccggttc
      781 gttcgatggc gtgcagcttc cgttcccgta tcgcggtgcc gagctcgggt tggagggcgg
      841 cgtaggcgcc accggcgccg cagcacagac cgtcgtcgtc gagttcgacg acggggacgt
      901 agcggctgag gatggcgcgg acggcgaggt gggccttctg gacgtggcgg agatggcagg
      961 ggtcgtgcac ggcgacgacg ggcgggaccg gcccggccac ggtggccggg agacggtcga
     1021 ggcgcgccgc gagccactga tgaacgtcca ccacgcgccg cgagaaggcc cgggcctcgt
     1081 cggtgccgag gaggtgcccg tagtcctgga gggcggcccc gcacccggcg gagtcgacga
     1141 ggatgggcgc atctccgggg aacgcctcga cgacgcggcg ggcgagcgct tgggcgcggt
     1201 cgtggagccc ggcgtggacg tgcagcgcgc cgcagcacgg cgcccggcgc gggtcgggga
     1261 tcgcgacgtc gactcccgcg gcgcgaagga cccgcatgac ggcggcgtgg acctcgcgtt
     1321 gccacgcgtc catgacgcag ccggggaaca gccagacgtc gtggccggtg gcggtcaggg
     1381 gcgacgatcg aagcgggagg tgcgggacgc cgagccgtct cggcaccagc cgggcccgct
     1441 gcgcgagagc gaggaggcgc gacagaccga cgagcagacg gtgatgctcg aggacggagt
     1501 agccgagccg ctgccaccag gggacggtcg aggtctcggc ggcgagggtc tcgcgggcgc
     1561 cttccatgag gtgcccgaac gccaccgacg acgggcaggc tgcctcacac gcccggcact
     1621 ggacgcattc cgtcatgtag cgggtgaact cggtgtcgag gggcgcggtt ccggagtgca
     1681 cggcgcgcat ggccgcgatg cgaccgcggg gcgacgcgga ctcgtctccg gtcactcggt
     1741 acgtcggaca gtgcggcagg cagagtccgc acgccacaca ggtgacgagc tcctcgtcgt
     1801 tgaccgcgag ccgcgtcacc ggtggcgccg gtcgcggtcg agggcgtctc ctccgggtgg
     1861 ggccgggcga cgtccgaatc gcaccgcagg gtgtcgctgc acaagccccg ttctaaacga
     1921 tcgcggcggc tcggggcgcc ctccggccct ggcggccggt ccaggcgcga ggatgagcgc
     1981 atggcggatg gcacccaggc gcgactcgaa gggggtggag cggcgctgct cggcgaccac
     2041 cggcgtctcg cggtcgtcac cgcccggttg cgggtcgggg cgggtgtcgc cttcctgttg
     2101 accgcaggac cgctggcccg agtcacgttg ggctcgacga gccgagaagc acgcgccgcg
     2161 atgcgtctca ccggagcccg ggatctggcg ctcgggctgg gcgcgctgac cacggtgaag
     2221 gaacgcaccc aggacgccga gtgggtgagc atgggcgcgc tcgtcgacgg gctcgacgcg
     2281 gtggtgctcc tcggaacgcg gcgactcccg cttcgagcac gcctgatcgg tgtcatcgcg
     2341 gccatcaccg ccgtcacggg actctgggtc tcgcagcgcc tcgctgacga acgcaccgat
     2401 ccggatcggt ccacctagct cccgagggtc aggcgtcgag cgtcctcggc gggtatgcga
     2461 cgatgcgcgc gtgccgatcg tgatcacctc ccccgacgat ctcgtcaccc gcgtcggact
     2521 cccgctgggc gtcagcgggt ggcatcgcgt ctcgcagg
//
LOCUS       DATCCH010000308         3664 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_57724, whole genome shotgun sequence.
ACCESSION   DATCCH010000308 DATCCH010000000
VERSION     DATCCH010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57724"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="VIJ44_05880"
     CDS             complement(<1..354)
                     /locus_tag="VIJ44_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165460.1"
                     /translation="MRPGRAVLALLLGVASVVAAAEPAGAHGVAGIQPSNFDTRVLAV
                     VPVTTGIHVQAVDLGNKLQLDNHTGRDVVVLGYDDEPYLRVGPAGVFENVRSPAVYLN
                     RTRTGNVPVPASADPH"
     gene            complement(354..878)
                     /locus_tag="VIJ44_05885"
     CDS             complement(354..878)
                     /locus_tag="VIJ44_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019874733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY5165461.1"
                     /translation="MDELTQLLLAARDGDRAALRAFVRASQGDVWRFVAHRVGSDDAD
                     DVTQDVYVRAWRAIPAFRHDATARTWLLAIARRACVDALRRRGRRFRLTERVAGAPTT
                     GSSPDLAAAHALEDLIAGLTGDRRDAFVLTQLLGCSYDEAATVCDVPVGTIRSRVARA
                     RADLLAQLRDVAAS"
     gene            complement(1239..1673)
                     /locus_tag="VIJ44_05890"
     CDS             complement(1239..1673)
                     /locus_tag="VIJ44_05890"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEY5165462.1"
                     /translation="MTDDLRDTVVSRLHGVHQRLTPNREAIIDILATTSRPLTIPEVL
                     DARDGLAQSSVYRNLVVLVEAGVVHRIVTNDEFARFELAEDLVGHHHHLICSVCGMVE
                     DVPPNARLEQSLRHAVAQIADATGFHTHAHRIDLVGLCRRCA"
     gene            1699..2466
                     /locus_tag="VIJ44_05895"
     CDS             1699..2466
                     /locus_tag="VIJ44_05895"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HEY5165463.1"
                     /translation="MQPTEEFTRLVRRPDREIPLDEAALLISAHDHDVDIAATLASLD
                     ELSRDAPDEPEAIAEFLFADGRFAGNEIDYADPRNSFLDEVLRRRLGIPITLSVLMIE
                     VSRRRGHTLPGIGMPGHFLVGAGPDRFIDPFHSGAALDTAGCRALFERLQIGAPWTDG
                     YLTPVGPRAILVRMLANLVHTLVERAPANAVWALRLRLAVPGVAAGERRDAAALLGTL
                     GRFEEAATVLDDLAGELDEPGAARVRQDAARLRARAN"
     gene            2498..3115
                     /locus_tag="VIJ44_05900"
     CDS             2498..3115
                     /locus_tag="VIJ44_05900"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEY5165464.1"
                     /translation="MPAAMPMFPLGTVLFPHGVLPLHVFEPRYRALTARCLADSAVFG
                     VVLIERGSEVGGGDHRFAVGTTARIVQAGRVVDGRWVLVTVGEQRVRVSQWLLDDPYP
                     IADVDVLDEHGGPTAAALIPDIGRALQRLLALRTELGESGPGVDVTLDDDPVRASFEA
                     CALASLGPFDAQQLLELDDTAERLTRLAELVDEETTVLELRLAEG"
     gene            3140..3280
                     /locus_tag="VIJ44_05905"
     CDS             3140..3280
                     /locus_tag="VIJ44_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165465.1"
                     /translation="MATTDDELRTEEADRDLATLEAAERELADLERDLSRIEGSEDAA
                     AG"
     gene            3315..3557
                     /locus_tag="VIJ44_05910"
     CDS             3315..3557
                     /locus_tag="VIJ44_05910"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEY5165466.1"
                     /translation="MRFFAAARDAAGTRELTVIAQPLGSLLEELRAGQGPEFAAVLAT
                     ARVWVNGEDPPDGDATRLAPGDEVAILPPVSGGALP"
BASE COUNT          540 a         1249 c         1347 g          528 t
ORIGIN      
        1 gtgcgggtcc gccgatgccg ggacgggaac gttcccggtt cgggttcggt tgaggtacac
       61 cgcgggagac cgaacgttct cgaacacgcc agcgggaccg acgcggaggt acggctcgtc
      121 gtcgtagccg agcacgacga cgtcgcgccc ggtgtggttg tcgagttgca gcttgttgcc
      181 gaggtccacg gcctggacgt ggatgccggt cgtgacgggg acgacggcaa ggacccgagt
      241 gtcgaagttg ctcggttgaa taccggcaac accgtgcgct ccggccggct ccgcggccgc
      301 cacgacgctg gcgaccccca gtagcaacgc gaggacggcg cgcccgggtc gcatcagctg
      361 gcggcgacgt cgcggagctg ggcgagcagg tccgcccggg cccgcgcgac gcgcgagcgg
      421 atggtgccga cgggcacgtc acagacggtg gcggcttcgt cgtaggagca gccgagcagc
      481 tgggtgagca cgaaggcgtc gcggcggtct ccggtgagcc cggcgatgag gtcttcgagg
      541 gcgtgcgccg cggccaggtc ggggctcgag ccggtggtgg gggccccggc gacgcgttcg
      601 gtgagccgga accggcgtcc gcgccgacgc aacgcgtcaa cgcaggcccg gcgggcgatg
      661 gcgagtagcc aggttcgagc cgtcgcgtcg tgacggaagg cggggatcgc ccgccaggct
      721 cggacgtaca cgtcttgggt gacgtcgtca gcgtcatcgg aaccgacacg gtgggccacg
      781 aaccgccaca cgtcgccttg gctggcccgg acgaaggcgc gcagcgcggc gcggtcgccg
      841 tcgcgggccg cgagcaacag ttgcgtcagc tcgtccaccg cgagcgacca taccgagcag
      901 ggtggtgggc cgggcatcac ggtgtcgcca ccgggcgggt caggccagca gggcgtttcg
      961 ccagagatcg aggacaggct cgcggcggtt ggcgaacgcg gacgagcgag cgggtcgtcg
     1021 tcgatgaggg tgcgcacgaa tggggcgtcg gagagatcgg acaggaccgc gccgcacgag
     1081 gtggagaccg actgacgggc gtcgtacggg ccggcgccga cgggcgtcca tctccgccac
     1141 ggctggtggc gggccttgac gctgcgcggg gtctggctgg cgaacgttcg ccacgacggg
     1201 atccacctcg ccgccaaccg ggcgcgttgg cgcgcacgtc aggcgcagcg gcggcacaac
     1261 ccgacgaggt cgatgcgatg cgcgtgggtg tgaaatcccg tcgcgtcggc gatctgggcc
     1321 acggcgtggc ggagcgactg ctcgaggcgg gcattgggtg ggacgtcctc aaccatgccg
     1381 cacaccgagc agatgaggtg atggtggtga ccgacgaggt cctcggcgag ctcgaagcgg
     1441 gcaaactcgt cgttcgtgac gatgcggtgc accacaccgg cttcgacgag gaccacgagg
     1501 ttgcggtaca cgctgctctg ggccagcccg tctcgagcgt cgagcacttc agggatggtg
     1561 aggggacgcg acgtcgtggc gaggatatcg atgatggcct cgcgattcgg cgtcagccgc
     1621 tggtggacgc cgtgcagccg tgacacgaca gtgtcgcgga ggtcgtccgt cacgacggcg
     1681 agtgtaccgt cgagccagat gcagcccact gaggagttca cgcgcctcgt ccgccgcccg
     1741 gatcgagaga tccccctcga cgaagccgcg ttgttgatct cggcgcacga tcacgatgtc
     1801 gacatcgccg cgacgcttgc atcgctcgac gagctgtccc gcgacgcgcc cgacgagcct
     1861 gaagcaatcg ccgagtttct cttcgccgac ggacggttcg ccggcaacga gatcgactat
     1921 gccgacccgc gcaactcgtt cctcgacgag gtgctccgcc ggcgcctcgg gatcccgatc
     1981 accttgagcg tcctcatgat cgaggtgagc cgccgacggg gccacaccct gccgggaatc
     2041 ggcatgcctg gtcatttcct cgtcggcgcc gggccggacc gtttcattga ccccttccac
     2101 agtggagcgg ccctcgacac cgcggggtgc cgggcgctgt tcgagcggct gcagatcggt
     2161 gccccctgga ccgacgggta cctcacgccc gtcggtccac gggcgatcct cgtccgcatg
     2221 ctggccaatc tcgtgcacac cctcgtcgaa cgggccccgg ctaacgccgt ttgggcgctg
     2281 cgcctccgcc tggcggtacc cggcgtggcg gccggcgaac gtcgcgacgc cgcggcactg
     2341 ctcgggaccc tgggccggtt cgaagaggca gccaccgtgc tcgacgacct cgccggcgag
     2401 ctcgacgagc cgggtgccgc gcgagtccgt caggacgccg ctcggctacg cgctcgcgcg
     2461 aactgaacct caggagccga ccggtaccgt gcagccgatg cccgcagcga tgcccatgtt
     2521 tccgctcggg acggtcctct tcccgcacgg tgtgctcccg ctgcacgtct tcgagccgcg
     2581 gtatcgagcg ttgaccgcac ggtgcctcgc cgacagcgcg gtgtttggag tcgtcctgat
     2641 cgagcggggc agcgaggtcg gcggtggtga ccatcgcttc gcggtgggta ccacggcgcg
     2701 gatcgtgcag gccgggcgcg tcgtcgacgg ccgctgggtg ctcgtgaccg tcggagagca
     2761 acgagtgcga gtctcgcagt ggttgctcga cgacccgtac ccgattgctg acgttgacgt
     2821 gctcgacgag cacggcggtc cgaccgccgc cgccctcatc cccgacatcg gccgggcgct
     2881 ccaacgcctg ctcgcgctcc gcaccgagct cggggagtct ggcccgggtg tcgacgtcac
     2941 gctggatgac gacccggtcc gggcctcgtt cgaggcgtgt gcgctcgcgt ccctcgggcc
     3001 cttcgacgcg cagcagctcc tcgagctgga cgacactgcc gagcgcttaa cgcgcctcgc
     3061 ggagctcgtc gacgaggaga cgacggtgtt ggagctccgt ctcgccgaag gttgagcagc
     3121 ccgcgcctac gctggtggcg tggcgaccac cgacgacgag ctccgcaccg aggaagccga
     3181 ccgtgacctg gccaccctcg aggccgccga gcgcgagctc gccgaccttg agcgggacct
     3241 cagccgcatc gagggctccg aagacgctgc cgccggatga gcaccacgac gggaacgtcg
     3301 gcctgagcgt ccgggtgcgg ttcttcgccg cggctcgcga cgccgccggg acccgagagc
     3361 tcaccgtcat agcgcaaccg ttggggagcc tcttggagga gttgcgcgcc ggtcaggggc
     3421 cggagttcgc ggccgtgctc gccaccgccc gagtctgggt caacggtgag gacccgcccg
     3481 acggcgacgc cacacgcctc gcccccggtg acgaggtcgc gatcctcccg ccggtcagcg
     3541 gcggggcgct tccgtaacgc cattggtggc acgaacgagc catgccgcgc cggggagccg
     3601 aacaccttcg ccggtcgcgt aaggcgcgag cgcatcacgc accgcaccca aaactcgacg
     3661 tctg
//
LOCUS       DATCCH010000309         2562 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_116711, whole genome shotgun sequence.
ACCESSION   DATCCH010000309 DATCCH010000000
VERSION     DATCCH010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116711"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /locus_tag="VIJ44_05915"
     CDS             complement(<1..952)
                     /locus_tag="VIJ44_05915"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEY5165467.1"
                     /translation="MDFTLSEEQHLLRDTARNLLAKECPTALVRAHIDDPAVAAPLWD
                     RLRDFSALGDGLLTDLCLFTTETGYVAAPGPFFATAGLFAPLLAALGDERLPAVLAGE
                     TSGTVAVAGSSGVWDPNDETVKCFVPEADRVDWIAVVDAGPTVRVVPAGETSTRLVET
                     VDWSRRLFEVDTAAVSSVPRRLDGAALDQVLRRATVALAAEMVGTARRLFDMTLEYAK
                     ERKQFDVPIGSFQAIQHKLADVSLAIERSDSAAQYAAMTLDARDADAPRATHVAKAAA
                     GTAATRAAKDGIQVHGGIGYTWEHDLHLFIRRAYGSEYWLG"
     gene            complement(956..2137)
                     /locus_tag="VIJ44_05920"
     CDS             complement(956..2137)
                     /locus_tag="VIJ44_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY5165468.1"
                     /translation="MRLSFGPEVEDFRAELITFLDEHAPAEMLRGFDMMDTGAEERDQ
                     VIPDWARAWQATLFDHRWMIPAYPSDLGGRDATPIQTLVYLEEMARRRILRSLHFPGY
                     AIVAPSLLEFGNAEQQALVRPAIRGDTVWCIGMSEPNAGSDLAGLQTRAVLDGDRFIV
                     NGQKVWTSYAMVAQKCFVYVRTDPDVPKHKGISLLIVDMDTPGVDVRPLRHISGAANF
                     AEVFFTDVEVPATNLVGALNDGWRITQGSLAHERAGLWVEGVSRLEATVRSLADMVRR
                     RGLAGDAGVRRRIAEMYEEASSLRALGYKGFAGFAQGSSAPEHSYMKLATSELGKAAY
                     ELGMELQGPYGATRDPERGEEQGRWVNMFFMSFANTIAGGSSEIQRNIIAQRVLGLPR
                     A"
     gene            complement(2146..>2562)
                     /locus_tag="VIJ44_05925"
     CDS             complement(2146..>2562)
                     /locus_tag="VIJ44_05925"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5165469.1"
                     /translation="GQLNYAAAKAGIAAMTIVLARELERVGVRANAIAPVARTRLTED
                     LAGGIMEAKGGFDTFAPENAAAGAVWLASPLANGITGQVLKIQGGVAQIVRGWRPITE
                     ATDDKPWTIETLDAQRDVLFAKTDPGVPPFFPPVEG"
BASE COUNT          384 a          833 c          940 g          405 t
ORIGIN      
        1 tgccgagcca gtactccgag ccgtacgcac gccgaatgaa caagtgaagg tcatgctccc
       61 acgtgtagcc gatcccgccg tggacctgga tcccgtcctt ggccgcgcgg gtggcggcgg
      121 tacccgcggc ggccttcgcg acatgggtgg cgcgcggcgc gtcggcatct cgggcgtcga
      181 gggtcatcgc cgcgtactgc gcggcgctgt cggatcgctc gatcgccaag gagacgtcgg
      241 cgagcttgtg ctggatggct tggaacgatc cgatgggcac gtcgaactgc ttgcgttcct
      301 tggcgtactc cagggtcatg tcgaagagtc ggcgggcggt tccgaccatc tccgcggcga
      361 gcgcgacggt ggcgcgccgc agcacctggt cgagcgcggc accgtcgagc cgccggggga
      421 cgctcgagac agccgcggtg tcgacctcga acagacgcct cgaccagtcg acggtctcga
      481 cgagacgggt cgaggtctcc ccggcgggga cgacgcgtac ggtcggcccg gcgtcgacca
      541 ccgcgatcca gtcgacccgg tcggcttcgg gcacgaagca cttgacggtc tcgtcgttcg
      601 ggtcccagac gccgctcgaa ccggcgaccg cgaccgtgcc cgaggtctcg cccgcgagca
      661 cggccgggag tcgttcgtcg ccgagcgcgg cgagcagcgg cgcgaacagg ccggcggtgg
      721 cgaagaacgg gccgggagcg gcgacgtagc cggtctcggt ggtgaagaga cacaggtcgg
      781 tgagcagacc gtcaccgagc gccgagaagt cccgcagccg atcccacagc ggcgcggcga
      841 cggcgggatc gtcgatgtgc gcgcggacca gggccgtcgg gcactccttg gccagcaggt
      901 tccgggcggt gtctcgtagg aggtgctgct cctcggagag ggtgaagtcc atcgttcagg
      961 cccgggggag gccgagcacg cgctgggcga tgatgttgcg ttgaatctcg ctggagccac
     1021 ctgcgatcgt gttcgcgaag ctcatgaaga acatgttgac ccagcggccc tgctcttcac
     1081 cgcgttcggg gtcacgggtc gcgccgtacg ggccttggag ctccatgccg agctcgtagg
     1141 cggccttgcc gagctctgag gtcgccaact tcatgtacga gtgctccggt gccgacgacc
     1201 cctgcgcgaa gccagcgaac cccttgtagc cgagcgctcg cagcgaagag gcctcctcgt
     1261 acatctcggc gatgcggcgc cggacaccgg cgtcaccggc gagacctcga cgacggacca
     1321 tatcggccag gctccgcacg gtcgcttcga gtcgggacac gccttcgacc cacagccccg
     1381 cccgctcgtg cgcgagggac ccctgggtga tgcgccaccc gtcgttgagg gccccgacga
     1441 ggttcgtggc ggggacctca acgtcggtga agaacacctc ggcgaagttc gcggcgccgc
     1501 tgatgtggcg caagggtcga acgtcgacgc caggtgtgtc catgtcgacg atgaggaggc
     1561 tgattccctt gtgcttcggc acgtcggggt cggtgcgcac gtagacgaag catttctggg
     1621 ccaccatcgc gtagctggtc cagaccttct ggccgttgac gatgaaccgg tcgccgtcga
     1681 ggacggcgcg ggtctggagg ccggcgagat ccgagccggc gttgggctcg ctcatcccga
     1741 tgcaccagac ggtgtcgcct cggatcgccg gtcggaccag cgcctgctgc tcggcgttgc
     1801 cgaactcgag gaggctcggg gcgacgatcg cgtacccggg gaagtgcagc gagcggagga
     1861 tgcggcgacg ggccatctcc tcgaggtaca cgagggtctg gatgggggtg gcgtcgcggc
     1921 cgccaaggtc cgacgggtag gcgggaatca tccaccggtg gtcgaagagc gtcgcttgcc
     1981 atgcgcgggc ccagtcgggg atgacctggt cgcgttcctc ggcgccggtg tccatcatgt
     2041 cgaagccgcg cagcatctcg gcgggagcgt gctcgtcgag gaacgtgatc agctcggctc
     2101 ggaagtcctc cacttcagga ccgaacgaca gacgcatgag tcgggtcagc cttccaccgg
     2161 cgggaagaac ggtgggacgc ccgggtccgt cttcgcgaac aacacgtcgc gttgcgcgtc
     2221 gagggtctcg atcgtccacg gcttgtcgtc ggtcgcttcg gtgatgggcc gccagccccg
     2281 cacgatctgg gccacaccgc cttggatctt gaggacctgg ccggtgatgc cgttggcgag
     2341 gggagaggcg agccagaccg caccggcggc cgcgttctcg ggtgcgaagg tgtcgaaccc
     2401 gcccttggct tccatgatcc cgcccgcgag gtcttcggtg aggcgggtgc gggccaccgg
     2461 ggcgatggca ttggcccgga ccccgacacg ctcgagctcg cgggcgagca cgatcgtcat
     2521 ggcggcgatc cctgctttcg ccgcggcgta gttcagctgg cc
//
LOCUS       DATCCH010000310         2975 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_573072, whole genome shotgun sequence.
ACCESSION   DATCCH010000310 DATCCH010000000
VERSION     DATCCH010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573072"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="VIJ44_05930"
     CDS             <1..650
                     /locus_tag="VIJ44_05930"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY5165470.1"
                     /translation="KDAFVAVAADVDTRVVILTGAGRGFSAGLDLKASGSSPVSDGLH
                     GVAAAMRGQAYFADVIQTLRAIPQPVIAAINGVAVGGGFALTMGCDLRFAATSARFNV
                     QALRIGLSAGESGLSFLLPRLVSASVAFEWLLTNRFVDAAEAERVGLVARTVPDVDLL
                     AVTRAAAGELARHDRDALCLAKATMWANLLAPSLDAALVLESRAWTLPGGADDDR"
     gene            640..993
                     /locus_tag="VIJ44_05935"
     CDS             640..993
                     /locus_tag="VIJ44_05935"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY5165471.1"
                     /translation="MTDEADRARWDDAWRDVMRREPPPITDPYTQFTAELFDRVWGRP
                     GLGRRERRLVSLTAVALSGQREPLVYHLGAALDSEDLSVDELDEWVVHLAHYGGWPTA
                     AMAYGALREVGARRA"
     gene            complement(990..2165)
                     /locus_tag="VIJ44_05940"
     CDS             complement(990..2165)
                     /locus_tag="VIJ44_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003926319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY5165472.1"
                     /translation="MRIELDPEQQQLRAELRQYFEGLMTPERRAALTGGEAGGEAYRD
                     VVRQMGRDGWLGIGWPAEWGGQGRTPIEQYVFYDEANRAGAPLPLVTLNTVGPTLMRF
                     GSEDQKRDFLPKILAGELHFAIGYSEPGAGTDLASLRTRAVRDGEEYVINGQKIWTTG
                     GHDADWVWLACRTDPDAPKHKGISMILVPTDVPGFKHSPIWVLGGGHTNATYYEDVRV
                     PVGNRVGNENEGWKLITRQLNHERVALAPAGRLDRHLHAVRGWAQQTRLADGRRVIDQ
                     EWVQLTLARVYAVTEALKLANWRVASDLAEGSLNPADASAMKVLATEEQCRCLRLLME
                     VVGVTASIPAGSPGTVLRGQLEQAYRQAPVGTFGGGVNEVQREIIQMAGLGMPRAPR"
     gene            2267..>2975
                     /locus_tag="VIJ44_05945"
     CDS             2267..>2975
                     /locus_tag="VIJ44_05945"
                     /inference="COORDINATES: protein motif:HMM:NF017652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0043720 - 3-keto-5-aminohexanoate cleavage
                     activity [Evidence IEA];
                     GO_process: GO:0019475 - L-lysine catabolic process to
                     acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEY5165473.1"
                     /translation="MAYPVIVEAAINGTTTKARNPNVPRQPDEIADDALRCLDRGASI
                     VHNHADVVAVPGPEAAARYLEGWAPVLAARPDALLYPTTNFGPGVEGAYAHLEPLVET
                     AGLRIGIIDPGSVNLGGVDRDGLPAAPGFVYANSFGDIHFQAELCARLRVGPSMAIFE
                     PGWLRAALVWWRAGRLPQGAMVKLYFGGDHGYPTTGSERSGAPFGLPPTVRSLDAYCE
                     LLDGCDLPWSVAVIGGDL"
BASE COUNT          383 a         1085 c         1018 g          489 t
ORIGIN      
        1 tgaaagacgc gttcgtggcc gtcgccgcgg atgtcgacac ccgggtcgtg atcctcaccg
       61 gtgccggccg gggattctcg gcgggtctcg acctcaaagc gtcggggtcg tcgccggtca
      121 gtgacggcct ccacggcgtc gcggcggcga tgcgcggcca ggcgtacttc gccgatgtca
      181 tccagacgtt gcgcgccatt ccgcaaccgg tgatcgccgc gatcaacggc gtcgcggtcg
      241 gaggcggctt cgcgttgacg atgggctgtg atcttcgatt cgctgcgacc tcggctcgct
      301 tcaacgtcca ggcgctgcgc atcggactca gtgccggcga gtcgggcctg tcgttcctcc
      361 tcccacggct ggtctcggcg tcggtggcct tcgagtggct gttgaccaac cggttcgtcg
      421 acgccgcgga agccgaacgg gtcggcctgg tggcgcgcac cgtgcccgac gtcgacctcc
      481 tggcagtgac ccgggccgcg gctggggagc tggcccgcca cgaccgcgat gcgctgtgcc
      541 tggccaaggc caccatgtgg gccaacctgc tggcgccgag cctggacgcg gcgctggtgc
      601 tggaatctcg agcttggacc ctcccgggag gagccgacga tgaccgatga agccgaccgt
      661 gcccgctggg acgacgcctg gcgtgacgtc atgcgccggg agccgccacc gatcaccgat
      721 ccgtacaccc aattcaccgc cgagctcttc gaccgggtgt ggggacgtcc cggtctcgga
      781 cgccgcgagc gtcgactggt ctcactgacc gcggtcgccc tctcgggcca gcgcgagccc
      841 ctcgtgtacc acctcggcgc ggccctcgac tccgaggacc tcagcgtcga cgagctcgac
      901 gagtgggtgg tgcacctcgc ccactacggc ggttggccga ccgcggcgat ggcctacggg
      961 gcgctgcgtg aggtcggggc ccggcgcgct tagcgcgggg cgcggggcat gccgagcccg
     1021 gccatctgga tgatctcgcg ctgcacctcg ttgaccccgc caccgaacgt ccccaccggg
     1081 gcctgccggt acgcctgctc gagctggccg cgcagcaccg tgcccggcga gccggcgggg
     1141 atcgacgcgg tcaccccaac cacctccatg agcagacgga ggcaacgaca ctgctcttca
     1201 gtggccagga ccttcatcgc cgacgcgtcc gccgggttca gcgacccctc cgcgaggtcg
     1261 ctcgccaccc gccagttggc cagcttgagc gcctcggtca ccgcgtacac ccgcgccaag
     1321 gtgagctgca cccactcctg gtcgatgacc cgccggccgt cggccagcct ggtctgctgc
     1381 gcccatcccc gcaccgcgtg gaggtgccga tcgaggcggc ctgcgggagc aagcgcgacg
     1441 cgctcgtggt tgagctggcg ggtgatcagc ttccagccct cgttctcgtt cccgacccgg
     1501 ttcccgactg ggacccgcac gtcctcgtag taggtggcgt tcgtgtgccc gccgccgagc
     1561 acccagatcg gtgagtgctt gaagccgggg acgtcggtcg gcacgaggat catcgagatc
     1621 cccttgtgct tcggcgcgtc gggatcggtc cgacaagcca gccacaccca gtcggcatcg
     1681 tggccgccgg tcgtccagat cttctggccg ttgatcacgt actcctcgcc gtcgcgcacc
     1741 gcccgcgtcc gcaggctcgc caggtcggta cccgcgcccg gctcgctgta cccgatcgcg
     1801 aagtggagct ccccggcgag gatcttgggg aggaagtccc gcttctggtc ctcggagccg
     1861 aaccgcatca gggtcggacc gacggtgttc agcgtcacga gcggcagggg cgccccggcc
     1921 cggttcgctt cgtcgtagaa gacgtactgc tcgatcgggg tgcggccctg cccgccccat
     1981 tcggccggcc agccgatacc gagccatccg tcgcggccca tctgtcggac cacgtcgcgg
     2041 taggcctcgc cgcccgcctc gccgccggtc agcgccgctc gccgctccgg ggtcatgagg
     2101 ccttcgaagt actggcgcag ctcggcgcgg agctgttgct gctcggggtc gagctcgatg
     2161 cgcaccgctt tccttctttc cttaattccc ttggtcgccg cggcacggta ccgtcgcccc
     2221 cagcgaactg caacacgttg cagttttgtc gagattgggg ggaagggtgg cgtatccggt
     2281 catcgtcgaa gccgcgatca acggcaccac caccaaggcc cggaacccga acgtgccccg
     2341 gcaaccggat gagatcgccg acgacgcgtt gcgctgcctc gaccgcggcg cgtcgatcgt
     2401 ccacaaccac gccgatgtgg tcgccgttcc gggcccggag gctgccgccc ggtatctcga
     2461 agggtgggcg ccggtcctcg cggcccggcc cgacgcgctg ctctatccga ccaccaattt
     2521 cggaccgggc gtcgagggcg cgtatgcgca cctggagccg ctcgtcgaga ctgccggtct
     2581 gcggatcggg atcatcgacc ctggatcggt gaacctcggc ggcgtcgacc gcgacgggct
     2641 cccggccgcc cccgggttcg tgtacgccaa ctcgttcggc gacattcact tccaggccga
     2701 gctctgcgcc cgcctgcggg tggggccgag catggcgatc ttcgagccgg gatggcttcg
     2761 cgccgctctg gtctggtggc gcgccggtcg cctgccccag ggcgcgatgg tgaagctgta
     2821 cttcggcggc gaccacgggt acccgacgac gggctcggag cgcagtggcg ccccgttcgg
     2881 cctcccgccg acggtccgtt ccctcgacgc ctactgcgag ctgctcgacg ggtgtgacct
     2941 tccctggtcc gtcgcggtca tcggcgggga cctgc
//
LOCUS       DATCCH010000311         2712 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_323750, whole genome shotgun sequence.
ACCESSION   DATCCH010000311 DATCCH010000000
VERSION     DATCCH010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323750"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VIJ44_05950"
     CDS             complement(<1..193)
                     /locus_tag="VIJ44_05950"
                     /inference="COORDINATES: protein motif:HMM:NF036997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165474.1"
                     /translation="MTGAVWRVLPDVRAIDRVFDYRPGSADPVPVGTAVRVPLHGRRV
                     RGWLLAVGDDTDAPDQALLA"
     gene            complement(207..1403)
                     /gene="metK"
                     /locus_tag="VIJ44_05955"
     CDS             complement(207..1403)
                     /gene="metK"
                     /locus_tag="VIJ44_05955"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEY5165475.1"
                     /translation="MSDRYTFTSESVTEGHPDKMCDQISDSVLDAIFEEDPQSRVACE
                     SMATTGLVVVAGEISTNAYIDIPRVVRDTVRSIGYDRESYGFDGNTCGVITSIDEQSP
                     DIAMGVDKAAEQRAGDGELYDEIGAGDQGMMFGYACDETDDLMPMPIWLAHRLAHRLA
                     EVRKSGVLPYLRPDGKTQVTVEYEDGRPKRLSCVLISTQTGPGVDIDTLLKPDLLEHV
                     IRPLLPAQFVDDDFDVLCNPTGNFELGGPHADCGLTGRKIIVDTYGGMARHGGGAFSG
                     KDPTKVDRSAAYAVRHAAKNIVAAGIAHRCEVQVAYAIGVARPVSVMVETFGTSEIDP
                     AKLPDLIHEHFDLRPAAIIENLNLRRPIFRRTAAYGHFGRPEPTFSWERTDQANELRA
                     AAKSLA"
     gene            complement(1400..2638)
                     /gene="coaBC"
                     /locus_tag="VIJ44_05960"
     CDS             complement(1400..2638)
                     /gene="coaBC"
                     /locus_tag="VIJ44_05960"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEY5165476.1"
                     /translation="MTPTPEPRAALRGRVVVLGVTGGIAAYKAVDVCRQLVDAGAHVA
                     PVLTADAHRFVGALTFSALASEPVRSQLFDGSEPIPHTRLGRRADLIVVAPATATVLA
                     KYAAGIADDLLTATLLATRAPVLIAPAMHTEMWEHPAVQANVAALTERGVHVVGPDAG
                     HLAGGDEGPGRLAAPDAIVAAAAEVLAEGRDLTGVTILVTAGGTREAIDPVRFVGNRS
                     SGKMGHAVADAAAHRGARVTLVTTTNRPSAAGVEVIPVETADQMADAVFGRFVDVDVV
                     VMAAAVADFRPKVAETEKLKKHDGVPELVLEPTPDILATLGGRKAHQILVGFAAETGR
                     VEEHAAQKLAAKNLDLVVGNDVSAPDAGFEVDTNRATLVNSDGSTEELPLLPKTALAT
                     TILDRVRDRLHHQGHQERHS"
BASE COUNT          360 a          845 c         1076 g          431 t
ORIGIN      
        1 ccgccaggag cgcctggtcg ggggcgtcgg tgtcgtcgcc gaccgcgagg agccagcccc
       61 gtacccggcg gccgtgcaac gggacccgga ccgcggtgcc gaccggcacc gggtcggccg
      121 acccgggccg gtagtcgaag acgcggtcga tggcgcgcac gtccgggagg acgcgccaca
      181 cggccccggt cacggggcgg ggcggattag gcgagggact tggccgcggc gcggagctcg
      241 ttggcctgat ccgtgcgttc ccagctgaac gtgggctcgg ggcgtccgaa gtgcccgtac
      301 gccgcggtac ggcggaagat cgggcgacgc aggttgaggt tctcgatgat cgccgcgggt
      361 cggaggtcga agtgctcgtg gatgagatcg ggcagcttgg ccgggtcgat ttcggaggtc
      421 ccgaaggtct cgaccatgac cgacactggg cgggcgacac cgatggcgta ggccacttgg
      481 acttcacagc ggtgggcgat gccggcggcg acgatgttct tggccgcgtg ccgcaccgcg
      541 tacgccgcgg agcggtcgac tttggtcggg tccttgccgc tgaacgcgcc gccaccgtga
      601 cgggccatgc cgccgtaggt gtcgacgatg atcttgcggc cggtgaggcc gcagtcggcg
      661 tgggggccgc cgagctcgaa gttgccggtc gggttgcaga ggacgtcgaa gtcgtcatcg
      721 acgaactggg ctgggagcaa gggtcggatg acgtgctcga ggaggtcggg tttgagcagg
      781 gtgtcgatgt cgacgccagg tccggtctgg gtggagatga ggacgcagga gagacgcttc
      841 ggtcggccgt cttcgtactc gacggtgacc tgggtcttgc cgtccggtcg gaggtagggc
      901 agcacgcccg actttcggac ctcggccagg cggtgcgcga gacggtgcgc gagccagatc
      961 ggcatgggca tgaggtcgtc ggtctcgtcg caggcgtagc cgaacatcat cccctggtcg
     1021 ccggcgccga tctcgtcgta gagctccccg tcgccggcgc gctgctcggc ggccttgtcg
     1081 acgcccatgg cgatgtcggg agactgctcg tcgatgctgg tgatgacacc gcaagtgttg
     1141 ccgtcgaacc cgtacgactc gcggtcgtag ccgatgctgc ggacggtgtc gcgcacgaca
     1201 cgcgggatat cgatgtaggc gttggtgctg atctccccgg cgacaacgac gaggccggtg
     1261 gtggccatgc tctcgcacgc gacccggctc tgggggtctt cctcgaagat cgcgtcgagg
     1321 accgagtcgc tgatctggtc gcacatcttg tcgggatggc cctcggtcac agactcggag
     1381 gtgaaggtgt aacggtcgct cacgaatgcc tctcttgatg tccttggtgg tggagacggt
     1441 cgcgtacccg gtcgaggatc gtggtcgcga gggccgtctt ggggagcagc gggagctcct
     1501 cggtcgagcc gtccgagttc accagggttg cccggttggt gtcgacctcg aagccggcgt
     1561 caggggcgga gacgtcgttg ccgaccacga ggtcgaggtt cttggccgcc agcttctggg
     1621 ccgcgtgctc ctcgacccgg ccggtctcgg ccgcgaatcc gaccaggatc tggtgagcct
     1681 tgcggccgcc gagggtggcg aggatgtcag gggtcggttc gagcaccagc tcgggaacgc
     1741 cgtcgtgctt cttgagcttc tcggtctcgg cgactttggg ccggaagtcg gcgaccgcgg
     1801 cggccatgac gacgacgtcg acgtcgacga agcgtccgaa caccgcgtcg gccatctggt
     1861 cggcggtctc gacggggatc acctcgaccc cggcggcgct gggccggttc gtggtggtga
     1921 cgagcgtgac ccgggcgccg cgatgggcgg cggcgtcggc gacggcgtgg cccatcttcc
     1981 ccgaggagcg gttcccgacg aaccggaccg ggtcgatggc ttcccgtgtt cctccggcgg
     2041 tgacgaggat cgtgacgccg gtgaggtccc ggccctcggc gaggacctcc gccgcggcgg
     2101 cgacgatggc gtcgggcgcg gcgaggcggc ccgggccctc gtcaccgccc gccagatggc
     2161 cggcgtcggg tccgacgacg tggacgccgc gctcggtcag ggccgcgaca ttcgcttgga
     2221 ccgcggggtg ctcccacatc tcggtgtgca tcgccggcgc gatcagcacc ggggcccggg
     2281 tcgcgagcag cgtcgcggtg agcaggtcat ccgcgatgcc ggcggcgtac ttcgcgagga
     2341 cggtcgcggt ggcgggcgca acgacgatga ggtcagcgcg gcgcccgaga cgggtgtgtg
     2401 ggattggctc ggatccgtcg aagagctggg agcggacggg ctcggacgcc agcgccgaga
     2461 acgtcagggc cccgacgaag cggtgcgcgt cggcggtgag gaccggggcg acgtgggcgc
     2521 cggcgtcgac cagctggcgg cacacgtcca cggccttgta ggcggcgatg ccgccggtga
     2581 caccgagcac gacgacacgg ccgcgcagcg cggcgcgggg ttcgggagtc ggggtcatgg
     2641 cgatgcgccg ggttcggcgc ggcggctact tgtcggtcgg ctcggcggcg tcggcctcgg
     2701 ccgacacggc tt
//
LOCUS       DATCCH010000312         2148 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_323765, whole genome shotgun sequence.
ACCESSION   DATCCH010000312 DATCCH010000000
VERSION     DATCCH010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323765"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="VIJ44_05965"
     CDS             complement(<1..283)
                     /locus_tag="VIJ44_05965"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HEY5165477.1"
                     /translation="MLQVETIPRLRRPILVLAIVGWVDAGAAGSSAASVLTSQLDGAR
                     VFARYPLDELVDLQQTRPTVRLVDGQTREIAWPTIEFVAGRAERDVVCCT"
     gene            319..1239
                     /locus_tag="VIJ44_05970"
     CDS             319..1239
                     /locus_tag="VIJ44_05970"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03560 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEY5165478.1"
                     /translation="MRFGVHTGLQNATTDELRSLWRHLEQQGFEWISIWDHFYAADAT
                     LTEAGTTSGSVCHEAVAAHTALALETRRVRCGSLVYCVAYRHPAVLANSMATIDHLSG
                     GRVTLGLGAGWHQGEFAAYGIPFHPVPVRLRMLDEAVQCIRLLLTEDTADFDGEFFTL
                     RDARCDPKPVQARLPIWIGGGGEKVTLRLAARHADGWNVPFITADSYRHKVEVLEGHC
                     AREGRDPGTIEKSVNLTLAWSDEELRARFGGMADWIRPSALTGSTDQIVDRIGEYRDG
                     GAEWVNLALRAPFDVEAIDRFAAEVLPAFR"
     gene            1236..>2148
                     /gene="galT"
                     /locus_tag="VIJ44_05975"
     CDS             1236..>2148
                     /gene="galT"
                     /locus_tag="VIJ44_05975"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HEY5165479.1"
                     /translation="MSTADGELRLDALTGEWVTIVGARQDRPHLPADGCPFCVGGLEA
                     PEPYTVRAFTNRWPPLTEGPPLEAAAGLTADTGRVPARGAAEIVLYTPEHDASFASLG
                     VDGARRVVDLWAERTVALLTRPDVAYVLVLENRGREVGATIDHPHGQIYGFPFVPPVP
                     AREATRSAEHGCVICAEVTRERADTDRVVMDRDGWLGWVPFASGWPYGMLLAPVEHVT
                     GLPSLTDEGRTGLAEVLVDGLGRYDRLFDRPVPYMLWVHQQPDEPDAHLHVHLAPTWR
                     AASVVRSVAAAELGSGTLFNPVVPEQAA"
BASE COUNT          298 a          748 c          782 g          320 t
ORIGIN      
        1 cggtgcagca caccacgtca cgctcagcgc gcccggcgac gaactcgatc gtcggccacg
       61 cgatctcacg ggtctgaccg tcgacgagcc gcaccgtcgg gcgggtctgt tggaggtcga
      121 cgagttcatc gaggggatag cgggcgaaca cgcgcgcccc gtcgagctgg gaggtcagca
      181 ccgaggcggc ggacgagccg gcggcgccgg cgtcgaccca accgacgatg gcgagcacga
      241 ggatcgggcg ccgcaagcgc gggatggtct cgacctggag cacgacatca cggtatcggc
      301 cctggatatg gtgcctcggt gcgcttcggg gtccataccg gtctgcagaa cgccaccacc
      361 gatgagctgc gatcgctgtg gcgtcacctc gaacagcaag gattcgagtg gatctcgatc
      421 tgggaccact tctacgccgc ggacgcgacc ctgaccgaag ccggcacgac atcggggtcg
      481 gtgtgccacg aggcggtcgc ggcgcacacc gcgctggcgc ttgagacccg tcgcgtccgc
      541 tgcggcagcc tcgtgtactg cgtcgcctac cgccacccgg cggtgctcgc caactcgatg
      601 gcgacgatcg atcacctctc gggtgggcgg gtcacgctgg ggctcggcgc cggctggcac
      661 cagggcgagt tcgcggcgta cgggatcccg ttccacccgg tgccggtccg gctgcggatg
      721 ctcgacgagg cggtgcagtg catccggctg ctgctgaccg aagacaccgc ggacttcgac
      781 ggtgagttct tcaccttgcg cgacgcgcgg tgcgacccga aaccggtgca ggcccggctc
      841 ccgatctgga tcggtggtgg cggtgagaag gtcacgttgc ggctggcggc ccgtcacgcc
      901 gacggctgga acgtgccgtt catcaccgcg gactcctacc gccacaaggt cgaggtcctc
      961 gaggggcact gtgcccgcga aggtcgcgac cccggaacga tcgagaagag tgtgaacctg
     1021 acgttggcgt ggagcgacga ggagctccgg gcccgcttcg gtgggatggc cgactggatc
     1081 cgtccgagcg ccctgaccgg cagcaccgat cagatcgtcg atcggatcgg cgaataccgc
     1141 gacgggggcg ccgaatgggt caacctggcg ttgcgtgccc cgttcgatgt cgaggcgatc
     1201 gaccggttcg cggccgaggt gctgcccgcg ttccggtgag caccgcggac ggcgaactgc
     1261 ggctcgacgc gttgaccggc gagtgggtga cgatcgtcgg cgcccgccag gatcgcccgc
     1321 acctcccggc cgacggctgc ccgttctgcg tcggtggtct cgaggccccc gagccgtaca
     1381 cggtgcgggc gttcacgaac cgctggcctc cgctcaccga gggaccgccc ctggaggccg
     1441 cggcgggcct caccgccgac accggccgcg tgcctgcacg cggcgcggcc gagatcgtcc
     1501 tctacacgcc cgagcacgac gcgtcgttcg cgtcgcttgg tgtcgacggg gctcgacggg
     1561 tggtggacct gtgggccgag cgcaccgtgg cgctcctcac ccgccccgac gtcgcgtacg
     1621 tcctcgtgct cgagaaccgg ggccgggagg tgggcgcgac gatcgaccat ccgcacggcc
     1681 agatctacgg gttcccgttc gtgccgccgg ttcccgcgcg ggaagcgacg cggtcggccg
     1741 agcatgggtg tgtgatctgc gccgaggtca cccgcgagcg ggccgacacg gatcgggtcg
     1801 tcatggaccg cgacggctgg ctgggctggg tgccgttcgc ctccgggtgg ccgtacggga
     1861 tgctcctcgc gccggtcgag cacgtcactg gcctcccgtc gttgaccgac gagggccgga
     1921 ccgggctcgc cgaggtcctc gtcgacgggc tcgggcgcta cgaccggctc ttcgaccgtc
     1981 cggttcccta catgttgtgg gtgcaccaac agcccgacga gcccgacgcc cacctccacg
     2041 tgcatctcgc gccgacgtgg cgggccgcgt cggtggtccg ctcggtcgcc gcggccgaac
     2101 ttggcagcgg caccctgttc aacccggtgg tcccggagca ggccgccg
//
LOCUS       DATCCH010000313         2254 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_431937, whole genome shotgun sequence.
ACCESSION   DATCCH010000313 DATCCH010000000
VERSION     DATCCH010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431937"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="VIJ44_05980"
     CDS             complement(<1..719)
                     /locus_tag="VIJ44_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEY5165480.1"
                     /translation="MSIFVDENTKVVYQGLTGSQGRFYGVLNREYGTQVVAGTNPKKA
                     GSDVDGIPVFATAAEAVKATGATASCVFIPAPGVRAAIEDAAQGGIEFIVVITEGVPM
                     HDEAWFFNELRRDYPKVRLLGPNCPGIISPGQCNIGITAGHIAKAGGPVGIVSRSGTL
                     TYQALYELKQQDIGVTTCVGIGGDPVPGTSFIDCLQAFETDPATKAVMMIGEIGGSAE
                     EEAAEYIKTEMSKPVVAYIAGV"
     gene            complement(727..1875)
                     /gene="sucC"
                     /locus_tag="VIJ44_05985"
     CDS             complement(727..1875)
                     /gene="sucC"
                     /locus_tag="VIJ44_05985"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013808370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEY5165481.1"
                     /translation="MDLFEYQGKQLFARYDIPVSPGAAAHTVDEAVAAADGIGYPVVV
                     KAQVHVGGRGKAGGIQLAANAAECRRHAQAILGMDIKGHIVGLVWVEKASDIAHEYYA
                     SFTLDRSAKMHLGMLSAEGGVEIEDVAVTNPDAIARIHVDPVDGLTATQCEEWVSAAG
                     LDEAARAGTIEILTKLYRCYEDADADLVEINPLILTPDGVVRALDAKVTLDANSVFRH
                     PEYKDYEATQPRDTREEEAYSKGLQYVGLDGSVGVIANGAGLAMSTVDVVNEVGGKPA
                     NFLDIGGGANADVMAAALEVINHDPAVKSILINIFGGITKGEEVANGIIQALERVAID
                     SPIVMRLDGTNADEGRQILQPHLSEKLRLEPTMLDAARAAVELARNAR"
     gene            complement(1920..>2254)
                     /locus_tag="VIJ44_05990"
     CDS             complement(1920..>2254)
                     /locus_tag="VIJ44_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443172.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HEY5165482.1"
                     /translation="RDAGFEVIYTGLFQTPDQVAEACLQEDADAVGLSVLSGAHLTLF
                     PKVVSLLRALGRDDVLVFGGGIIPGDDVTALEAAGVARVFTPGAPLGDITDWLEQALD
                     ARESRVAH"
BASE COUNT          332 a          730 c          771 g          421 t
ORIGIN      
        1 accccggcga tgtacgcgac gactggcttg ctcatctcgg tcttgatgta ctcggcggct
       61 tcttcttcgg cggatccgcc gatctcgccg atcatcatga cggcctttgt ggccgggtcg
      121 gtttcgaagg cctggaggca gtcgatgaag ctggtcccgg gcaccgggtc gcctccgatg
      181 cccacacagg tggtgacgcc gatgtcttgc tgtttgagct cgtagagcgc ttggtaggtg
      241 agggtccccg accggctcac gatgccgacg gggccgccgg ccttggcgat gtggccggcg
      301 gtgatcccga tgttgcactg tccggggctg atgatccccg ggcagttcgg gccgagcagg
      361 cgcaccttcg ggtagtcgcg gcgcagctcg ttgaagaacc aggcttcgtc gtgcatcggg
      421 acaccttcgg tgatgaccac gatgaactcg atgccgcctt gcgcggcgtc ttcgatggcg
      481 gctcggacgc cgggagcggg gatgaagacg cacgacgcgg tggcgccggt ggccttcacc
      541 gcctcggcgg cggtggcaaa caccgggatg ccgtccacgt cgctgccggc cttcttgggg
      601 ttggtgccgg cgacgacctg ggtcccgtac tcgcggttca agaccccgta gaaccgtccc
      661 tgtgatccgg tcagaccttg gtagacgacc ttggtgttct cgtcgacgaa aatgctcact
      721 ggcggttcac ctcgcgttgc gggcgagctc gaccgcggcg cgcgccgcgt ccagcatggt
      781 cggctcgagt cgcagctttt cggacaggtg gggctggagg atctgtcgac cttcgtcagc
      841 attggtgcca tcgaggcgca tcacgatcgg agaatcgatc gcgacccgct ccagggcctg
      901 gatgatcccg ttggcgacct cttctccctt ggtgatgccg ccgaagatgt tgatgaggat
      961 cgatttcacg gcgggatcgt ggttgatgac ctcgagcgcg gcggccatca cgtcagcgtt
     1021 ggcgccgccc ccgatgtcga ggaagttcgc cggcttcccg cccacctcgt tgacgacgtc
     1081 gacggtgctc atggcgagcc cggcaccgtt ggcgatgacc ccgacgctgc cgtcgaggcc
     1141 gacgtactgc aggcccttgc tgtaagcctc ttcctcgcgg gtgtctcgag gctgggttgc
     1201 ctcgtagtcc ttgtactcgg gatggcggaa caccgagttg gcgtcgaggg tcaccttggc
     1261 gtcgagcgcc cgcacgacgc cgtcgggggt caagatgagc gggttgatct cgacgaggtc
     1321 ggcgtcggcg tcctcgtagc accggtagag cttggtgagg atctcgatcg tgccggcgcg
     1381 tgccgcttcg tcgaggccag cggcactcac ccactcctcg cactgcgtcg cggtcagccc
     1441 atccaccggg tcgacatgga tgcgggcaat ggcgtcgggg ttcgtgaccg cgacgtcttc
     1501 gatctccacc ccgccttcgg ccgagagcat cccgagatgc atcttcgctg accggtcgag
     1561 ggtgaacgac gcgtagtact catgggcgat gtcgctcgcc ttctcgaccc acaccaaccc
     1621 aacgatgtgg cccttgatgt ccatcccgag gatggcctgc gcgtggcggc gacactcggc
     1681 ggcgttggcc gcgagttgga tcccgcctgc cttgcctcgg ccccccacgt ggacctgggc
     1741 tttcacgacg acggggtagc cgatcccgtc ggccgcggcg acagcttcgt cgacggtgtg
     1801 cgccgccgcg cccggcgaca cggggatgtc gtagcgcgcg aacagctgct tgccttggta
     1861 ttcgaagaga tccatgtagc tcctgatcga gaagagaact cggtgtcgtc ggtgtcggcc
     1921 tagtgggcca cccgcgactc ccgggcgtcg agcgcctgct ccaaccagtc ggtgatgtca
     1981 ccgaggggcg cgccgggggt gaacacccga gcgacgcccg cagcctcgag tgccgtcaca
     2041 tcgtcaccgg ggatgatccc gccgccgaac accaagacgt cgtcgcggcc gagtgcgcga
     2101 agcaggctca cgaccttggg gaacaacgtc agatgggcgc ccgacagcac cgagaggccc
     2161 accgcatcgg cgtcttcttg gaggcacgcc tcggcgacct gatcgggggt ctggaacagc
     2221 ccggtgtaga tgacctcgaa gccggcgtct cgca
//
LOCUS       DATCCH010000314         2187 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_491384, whole genome shotgun sequence.
ACCESSION   DATCCH010000314 DATCCH010000000
VERSION     DATCCH010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491384"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VIJ44_05995"
     CDS             complement(<1..312)
                     /locus_tag="VIJ44_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEY5165483.1"
                     /translation="MNELHRSVAAPPVRHVHLGLGNFFRAHQAWYGTHAPDAAEWGIA
                     AFTGRGSKRSNAVAAALAAQDGLYTLITRASDGDRFEVISSIARVHTAADHAVWLDYF
                     AD"
     gene            complement(309..1733)
                     /gene="uxaC"
                     /locus_tag="VIJ44_06000"
     CDS             complement(309..1733)
                     /gene="uxaC"
                     /locus_tag="VIJ44_06000"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014741062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEY5165484.1"
                     /translation="MQSSRAFPLSLHPDRLLPVDPSVRDIARRLYKTVQDLPIISPHG
                     HVDPRILLDDVPFRDPAQLFVTPDHYVTRLLHASGVPLDALGVGQGPLSETAAREAWR
                     LLCSHWHIYRATPVRYWLESELAEIFGVAVRPSAGTADAIYDHLAERLTQDAYRPRAL
                     FTRFNISVLATTDDPCDDLSAHSALVADPTWSGRVIPTFRPDRYLEASEAGWPAAVER
                     LGEVSGVDTGTYSGWVSAMEERRRYFIEHGARSADHAHTDAGTEPLETSEVDRIYRVA
                     KAGTATPSEAVALRRHMLLEMARMSTQDGLTMTLHVGVRRGHHGPSNAKFGPDTGHDI
                     PLRGDFTDPLRPLLERYGTHPNLKLVLFTLDETVFSRELAPLAGFYPSVYIGAPWWFL
                     DAPDAVRRWRSAVTDTAGFSRTSGFIDDTRAFCSIPARHDMSRRLDSGHLAQLVAEHR
                     MDEDEAQETVVDLVTGRPQDVFKL"
     gene            complement(1753..>2187)
                     /locus_tag="VIJ44_06005"
     CDS             complement(1753..>2187)
                     /locus_tag="VIJ44_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HEY5165485.1"
                     /translation="LEKSLGALAKAGQADLCAVYEYAEPVTERGLVFMDTPGYDPVSV
                     TGIVAGGATVVCFTTGRGSVFGCRPTPSIKLATNTQMYQRMAEDMDLNCGRIVDGTAT
                     LTQVGDEIFERIIAVASGERTVSEELDIGQEEFIPWQLGTVT"
BASE COUNT          357 a          715 c          776 g          339 t
ORIGIN      
        1 gtcggcgaaa tagtcgagcc agactgcgtg atcggccgcg gtgtgcaccc tggcgatgct
       61 gctgatcacc tcgaaacggt caccatcgct cgcccgggtg atcagcgtgt acaggccgtc
      121 ctgcgcagcg agcgcagccg cgaccgcgtt cgagcgcttc gagccgcgac ccgtaaaggc
      181 ggcgatgccc cactcggccg catccggggc atgagtgccg taccaggcct ggtgggcgcg
      241 gaagaagtta cccaagccga ggtggacgtg tcggacgggt ggcgccgcca cggacctgtg
      301 gagctcgttc acagcttgaa gacgtcctga gggcgacctg tcacgaggtc aaccaccgtt
      361 tcttgggcct cgtcctcgtc catccggtgt tcggcgacca gctgggcgag atggccgctg
      421 tccaggcggc gggacatgtc gtgccgcgcc gggatggagc agaacgcgcg ggtgtcatcg
      481 atgaagcccg acgtccggga gaagccggcg gtgtcggtga ccgccgagcg ccagcgccgc
      541 accgcgtcgg gggcgtccag gaaccaccag ggcgccccga tgtacaccga cgggtagaag
      601 ccggccagcg gcgccagctc gcgtgagaac acggtctcat cgagggtgaa gagcaccagc
      661 ttcagattgg ggtgggtgcc gtaccgctcg agcaggggcc ttagcgggtc ggtgaagtcg
      721 ccgcgcagcg ggatgtcgtg accggtgtcc ggtccgaact ttgcgttcga tggaccgtgg
      781 tgaccgcgcc gcacgcccac gtgcagcgtc atggtcaggc cgtcctgtgt cgacatccgc
      841 gccatctcga gcaacatatg ccgtcgcaag gccaccgcct cggagggcgt ggctgtgccg
      901 gccttggcca cccggtagat ccggtcgacc tcggaggtct cgagcggctc ggtacccgcg
      961 tcggtgtgag cgtgatcggc ggacctggcg ccatgctcga tgaagtagcg acgccgttcc
     1021 tccatggccg agacccagcc ggagtacgtg cccgtgtcaa cgcccgagac ctcgcccagg
     1081 cgctcgacag cggccggcca gccggcctcg gacgcctcga ggtagcgatc ggggcggaac
     1141 gtcgggatga ctcgtcccga ccaggtcggg tccgcgacca gcgcggagtg cgcagagagg
     1201 tcgtcacacg ggtcgtccgt cgtggcgagc accgagatgt tgaagcgggt gaacagcgca
     1261 cgcggccggt aggcgtcctg ggtcagccgt tcggcgaggt ggtcgtagat ggcgtcggcc
     1321 gtgccggccg aaggccggac cgcgacgccg aagatctcgg ccagctcgga ctccagccag
     1381 tacctcaccg gagtggcgcg gtagatgtgc cagtgcgagc agagcagccg ccaggcctcg
     1441 cgcgcagccg tctcgctgag cgggccctgc ccgaccccga gagcgtccag cggcacaccg
     1501 gaggcgtgca gcaaccgggt cacgtaatgg tccggggtca cgaacagctg cgcggggtct
     1561 cggaacggca cgtcgtccag caggatccgc gggtccacgt gcccgtgcgg ggagatgatc
     1621 gggagatcct gcaccgtctt gtagagccga cgggcgatgt cacggaccga ggggtccacg
     1681 ggcaggagcc ggtccgggtg caaggagagc ggaaacgctc ggctgctctg catgtctttc
     1741 atgtcttcct tttcatgtca cagtgccgag ctgccagggg atgaactcct cctggccgat
     1801 atcgagctcc tcgctgacgg tgcgttcgcc cgaggcgacg gcaatgatgc gctcgaagat
     1861 ctcgtcgccc acctgggtga gcgtggccgt gccgtcgacg atccgaccgc agttgaggtc
     1921 catgtcctcg gccatccgct ggtacatctg tgtgttggtg gcgagcttga tcgacggcgt
     1981 gggacggcat ccgaacaccg atccacgccc ggtcgtgaaa cagaccacag tggccccacc
     2041 ggcaacgatc cccgtcaccg agaccgggtc gtacccagga gtgtccatga acacgagtcc
     2101 gcgctcggta accggctccg cgtactcgta caccgcgcag agatcggctt gcccggcctt
     2161 cgccagggcg cccagcgact tctccag
//
LOCUS       DATCCH010000315         2321 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_117371, whole genome shotgun sequence.
ACCESSION   DATCCH010000315 DATCCH010000000
VERSION     DATCCH010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117371"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="VIJ44_06010"
     CDS             complement(<1..704)
                     /locus_tag="VIJ44_06010"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018653369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEY5165486.1"
                     /translation="MRTRATEMFGLELPVFAFSHCRDVVAAVSKAGGLGVLGALAFSP
                     EQLEIELNWIDEHVDGKPYGVDVVMPASYAGSGGELDPDRMEEQLSDMIPQGHKDFIE
                     RVLDEYEVPPLGDDGDDRSGLLGWTHEGARPQVEISLAHPIKLLVNALGPPPKDVVDL
                     AHEQGVKVAALVGSVAQAERQVNQGVDMIVAQGYEAGGHTGDVGVMVLIPEVVDAVGA
                     RVPVLAAGGVGSGRQMA"
     gene            complement(714..>2321)
                     /locus_tag="VIJ44_06015"
     CDS             complement(714..>2321)
                     /locus_tag="VIJ44_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020557815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEY5165487.1"
                     /translation="NLADLFEHAADHFASREYLVAEGQRRTYAEMEERANRLAHHLAD
                     HGIGPGDHVGIYAYNSVEWVETLWAVFKLRAVWVNINYRYVEGELTYLFENADLKALV
                     YQREFAPRVRNVIDTLPLLTHSIVIDDGSGADLDGLDSVDYEAAMASGSPVRDFGPRS
                     PDDRYILYTGGTTGMPKGVVWRHEDVFFALGGGIDPTTNTRVDRPEALVARAQEREPG
                     TMLPIAPLMHGATQWGVMGGAFQGNKIVLVARFDPDRVWQLVGEERVNGIMITGDAMG
                     RPLVEALDEPGADHDLSSLVSLSSTAAVFSPAVKERFLERLPNVILTEAIGASESGSN
                     GYTLVQSGEESTRGPKVKAILDTVVLDDDLRPVQPGSTVVGKVARKGNIPIEYYKDPE
                     KSAQTFVTAPDGTRYSIPGDFASVEADGRIVLLGRGSVSINSGGEKIYPEEVEAAIKS
                     HPDVYDCTVVGVPDDRWGQRVAAVVQPRESGTLTLEAIQEHCRQHVAGYKVPRELHLV
                     ASIVRSPSGKPDYRWAKDVATGVATSA"
BASE COUNT          358 a          794 c          763 g          406 t
ORIGIN      
        1 gccatctgac gccccgatcc caccccgccg gccgcgagga ccggaacccg ggctccgacg
       61 gcgtcgacca cctcggggat gagcaccatc acgccgacgt ctccggtgtg gccaccggcc
      121 tcgtacccct gggcgacgat catgtcgacg ccttggttca cctgtcgttc ggcttgggcg
      181 acgctgccga cgagcgcggc gaccttgacg ccttgctcgt gggcgaggtc cacgacgtcc
      241 ttcgggggtg ggccgagcgc gttgacgagc agcttgatcg ggtgggccag cgaaatctcc
      301 acctgcggac gcgcgccttc gtgggtccag ccgagcaggc cgctgcggtc atcgccgtcg
      361 tcgccgagcg gcggtacctc gtactcgtcg aggacgcgtt cgatgaagtc cttgtggccc
      421 tgcgggatca tgtcggagag ctgctcctcc atccggtcgg gatcgagctc tccgccggaa
      481 ccggcgtagc tggccggcat gacgacgtcg acgccgtagg gcttgccgtc gacgtgctca
      541 tcgatccagt tgagttcgat ctcgagttgc tcggggctga acgccagggc cccaaggaca
      601 ccgagtccgc cggccttgct caccgcggcg acgacgtcgc ggcagtggct gaacgcgaag
      661 acgggcagtt ccaacccgaa catctcggtg gctcgtgtgc gcacgatttc ctcctacgcc
      721 gaggtagcga caccggtcgc cacgtctttt gcccagcggt agtcgggctt tcccgacggt
      781 gaccgaacga tgctggcgac gaggtgcagc tcccgcggca ccttgtagcc ggcgacgtgc
      841 tgacggcagt gctcttggat cgcctctagg gtcagcgtcc cagattcccg gggctgcacc
      901 actgccgcga cccgctgacc ccaccggtcg tcggggacgc cgacgaccgt gcagtcgtac
      961 acgtcagggt gagacttgat ggccgcctcg acctcctcgg ggtagatctt ctcgcctccc
     1021 gagttgatgg acaccgatcc gcgtccgagc aacacgatcc ggccgtcggc ctccacgctc
     1081 gcgaagtcgc cggggatcga gtaccgggtt ccgtctggcg cggtcacgaa cgtctgcgcc
     1141 gacttctcgg gatccttgta gtactcgatc gggatgtttc ccttccgggc gaccttgccg
     1201 accacggtcg accccggttg gaccggacga aggtcatcgt cgagcaccac cgtgtcgagg
     1261 atcgctttga ccttcgggcc tcgggtcgac tcctcacccg actggaccag cgtgtacccg
     1321 ttcgaaccgc tctcggacgc gccgatcgct tcggtgagga tcacgttggg cagtcgctcg
     1381 aggaagcgtt ccttgaccgc gggcgagaac actgccgcgg tactcgacag cgacacgagc
     1441 gacgagaggt cgtggtcagc cccgggctcg tcgagcgcct cgaccagcgg gcgacccatc
     1501 gcgtcgccgg tgatcatgat cccgttgacc cgctcctctc cgacgagttg ccagacccga
     1561 tcgggatcga agcgggcgac gagaacgatc ttgttgccct ggaacgcgcc gcccatgaca
     1621 ccccattggg tcgcaccatg catgagcggc gcgatcggga gcatcgttcc cggctcgcgc
     1681 tcctgggccc gggccacgag tgcctcgggg cggtccaccc gggtgttggt cgtcggatcg
     1741 atcccaccac ccagggcgaa gaagacgtcc tcgtggcgcc acacgacgcc cttcggcatc
     1801 ccggtcgtgc ccccggtgta gaggatgtag cgatcgtcgg gtgagcgcgg cccgaagtcg
     1861 cgaaccggtg agccgctcgc cattgccgcc tcgtagtcga ccgagtcgag cccatcgaga
     1921 tccgctcccg agccgtcgtc gatcacgatc gagtgcgtca ggagcggcaa cgtgtcgatg
     1981 acgttccgga cccggggcgc gaactcccgc tggtagacga gggctttcag atccgcgttc
     2041 tcgaacagat aggtcagctc gccttcgacg tatcggtaat tgatgttcac ccacacggcc
     2101 cgaagcttga acaccgccca gagggtctcg acccactcga ccgagttgta cgcgtagatc
     2161 ccgacgtggt cgccgggccc aatgccatgg tccgctaggt gatgcgcgag acggttcgcg
     2221 cgttcctcca tctcggcgta ggtgcggcgc tgcccctcag cgaccaggta ctcgcgggac
     2281 gcgaagtggt cggcggcgtg ctcgaacagg tcagcgaggt t
//
LOCUS       DATCCH010000316         2590 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_390935, whole genome shotgun sequence.
ACCESSION   DATCCH010000316 DATCCH010000000
VERSION     DATCCH010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390935"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="VIJ44_06020"
     CDS             complement(<1..380)
                     /locus_tag="VIJ44_06020"
                     /inference="COORDINATES: protein motif:HMM:NF037644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165488.1"
                     /translation="MTTWASLFDGAERAFTAAGIATPAVDARWIIEEVTDRRDGAWVL
                     VADEDAPPQAVRRVHELVTRRARGEPLQYVVGSWGFRDLTLMVDPRALIPRPETEQTV
                     EIALGEAARLGLRIGRKDPWGGTET"
     gene            complement(377..1450)
                     /gene="prfA"
                     /locus_tag="VIJ44_06025"
     CDS             complement(377..1450)
                     /gene="prfA"
                     /locus_tag="VIJ44_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012818219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEY5165489.1"
                     /translation="MFERLADLETELEKLESRLPEIYASGDQRAARDAGRRHAQLRPI
                     VDGYRDFRGTEADLADAQELLSGEPDTEMREYLRGEIADKEQRLAQLAARLRELLVPN
                     DPNDGRNVIVEIRGAEGGEEANLWAADLTRMYEALARRHGWKTETLSTQPSDMGGFRE
                     VSLVVKGTDAWARLKHEAGPHRVQRVPVTESQGRVHTSAATVAVLPEAEELDVDIDPN
                     DLEIDVYRSSGPGGQSVNTTDSAVRMTHVPTGVVVTCQDEKSQLQNKDKALRILRSRL
                     LQLEQERQDAEVSSARRSQVKGGGRSEKVRTYNFKENRVTDHRVGVTVHALDQVLAGG
                     EPLDDITDALAAAERAEQLGAEA"
     gene            complement(1481..>2590)
                     /locus_tag="VIJ44_06030"
     CDS             complement(1481..>2590)
                     /locus_tag="VIJ44_06030"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEY5165490.1"
                     /translation="TLVVVELAGGNDGLNTVVPHADPAYHALRPTLGVTQPIDLDGQI
                     GFHPNLAKLAARYKAGQVAIVEGVGYTPPNLSHFQSLAVWWNAERSSDAGGWLGSYLD
                     ATVGYDDPLAGVAIGRQPAPALVGQHSFATTIADASGLQPRLPVWAGAPDSVLRAWGR
                     FAPARVDSRQLVGQVERAVGLTVKARTELDRVLHPGGATPPTAAPSPSALYGGTVADS
                     LRVAAQLIRSPLAPRVIYITGVGDFDFHQGEAQRHPALMSELDAGIDGFFGAVGDAAD
                     RALVITTSEFGRRPAENGSGTDHGTANSHFLIGNKVKGGRYGSPPSLTKLDATNNLVP
                     TTDFRTMYATGLTWLGVQDTEHILGGRFDPVGALI"
BASE COUNT          347 a          885 c          941 g          417 t
ORIGIN      
        1 gtctcggttc ctccccacgg atcctttcga ccgatgcgga gcccgagccg ggccgcctcg
       61 ccaagcgcga tctcgaccgt ctgctcggtc tcgggccggg ggatcaacgc ccgggggtcg
      121 accatgaggg tcagatcgcg aaacccccag gagccgacca cgtactgcag gggctcgccc
      181 cgggctcggc gtgtcacaag ctcgtggacc cgacgcaccg cctggggggg cgcgtcctcg
      241 tcagcgacca ggacccatgc gccgtcgcgc cgatcggtga cctcttcgat gatccagcgg
      301 gcatcgaccg ccggtgtggc gatgccggcc gcggtgaacg cgcgctcggc gccgtcgaac
      361 agcgacgccc aggtggtcac gcctcggcgc cgagctgctc ggcccgctcg gcggcggcga
      421 gagcgtcggt gatgtcgtcc aggggttctc cgccggcgag gacctggtcg agggcatgca
      481 ccgtgacgcc cacccgatgg tcggtgacgc ggttctcctt gaagttgtag gtccggacct
      541 tctcggatcg tccgccaccc ttcacctggc tgcgccgcgc cgacgagacc tcggcgtcct
      601 gtcgctcctg ctcgagctgc aacagacgag accgcaggat gcgcagcgcc ttgtccttat
      661 tctgcagctg gctcttctcg tcctgacagg tcacgacgac cccggtcggc acatgggtca
      721 tccgcaccgc cgagtccgtg gtgttcaccg actggccgcc gggcccgctg gagcggtaga
      781 cgtcgatctc gaggtcgttc gggtcgatgt cgacatcgag ctcttcggct tcggggagca
      841 cggcgacggt cgcggcgctg gtgtggaccc ggccctggct ctcggtgacc gggacgcgct
      901 ggacccggtg cggaccggcc tcgtgcttga gccgcgccca cgcgtcggtt cccttgacga
      961 cgaggctgac ctcgcggaag ccgcccatgt cggagggctg ggtggagagc gtctcggtct
     1021 tccagccgtg gcgacgggcc agcgcctcgt acatgcgggt caggtcagcg gcccacaggt
     1081 tcgcctcttc gccgccctcg gcgccccgga tctcgacgat gacgttccga ccgtcgttgg
     1141 ggtcgttcgg gacgagcagc tcgcggagcc gggccgcgag ctgcgccagt cgttgctcct
     1201 tgtcggcgat ctcaccccgc aggtattcgc gcatctcggt gtcgggctcc cccgagagca
     1261 gttcctgcgc gtcggcgagg tcggcctcgg tgccccggaa gtcgcggtac ccgtcgacga
     1321 tcggtcgaag ctgagcgtgg cggcgcccgg catcacgcgc ggcgcgctga tcgccggagg
     1381 cgtagatctc cgggagacgc gactcgagct tttccagctc ggtctcgaga tcagcgaggc
     1441 gttcgaacac cgcgccaggg taccgacacc ctgcgcgggc tcagatgagc gcgccgaccg
     1501 ggtcgaaccg gccgccgagg atgtgctcgg tgtcctggac gccgagccac gtgagccccg
     1561 tcgcgtacat ggttcggaag tcggtggtgg gcacgaggtt gttggtcgcg tcgagcttgg
     1621 tgagcgacgg gggggagccg tagcggccgc ccttgacctt gttgccgatg aggaagtggc
     1681 tgttggcggt tccatgatcg gttccgctgc cgttctcggc cggccgtcgc ccgaactccg
     1741 aggtcgtgat cacgagggcc cggtcggcgg cgtcgccgac ggcgccgaag aagccgtcga
     1801 tgccggcgtc gagctcggac atcagcgcgg ggtggcgttg cgcttcgccc tggtggaagt
     1861 cgaagtcacc gaccccggtg atgtagatca ctcgcggcgc gagcggggat cggatcagct
     1921 gcgcggcgac acgcagcgag tcggccactg tcccgccgta gagcgcactc gggctcggcg
     1981 cggcggtcgg cggggtcgca ccgcccgggt gcagaacccg gtcaagttcg gtgcgggcct
     2041 tcacggtcaa cccaacggcc cgctcgacct gcccgaccag ctggcggcta tcgactctcg
     2101 ccggcgcgaa ccgaccccag gcgcgcaaga ccgagtcggg cgcgccggcc cagaccggca
     2161 gacgcggctg gaggccgctg gcgtcggcga tcgtcgtcgc gaaggaatgc tgaccgacga
     2221 gggccggtgc gggctggcgc ccgattgcga ccccggctaa cgggtcgtcg tacccgacgg
     2281 tggcgtcgag gtagctgccc aaccatccgc cggcgtcgct cgaccgttcc gcgttccacc
     2341 acaccgccag cgactggaag tgcgagaggt tcggtggcgt gtagccgaca ccctcgacga
     2401 tggcgacctg gcccgccttg tagcgcgccg cgagcttcgc gaggttgggg tggaacccga
     2461 tctgaccgtc gaggtcgatc ggctgggtga cacccaatgt cggtcggagg gcgtgatacg
     2521 ccgggtcggc atgcgggacc acggtgttga gcccgtcgtt gcctccggcg agctcgacga
     2581 cgacgagggt
//
LOCUS       DATCCH010000317         4261 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_283657, whole genome shotgun sequence.
ACCESSION   DATCCH010000317 DATCCH010000000
VERSION     DATCCH010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4261
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283657"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="VIJ44_06035"
     CDS             <1..487
                     /locus_tag="VIJ44_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003931249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY5165491.1"
                     /translation="FVSDEAIEPVTLTLLPGAEFHQLWARDATASIPDDTTTTPRPAY
                     FPAVGGFRFGFFTLGPESVASPADIDIEAALNELTEKLPGMAEAMEPEHPGMHTTDTI
                     DFDIVISGEVWLELDDGAEVQLRAGDSVVQNGTRHAWHNRSSQPCTVAVALIGAARTP
                     G"
     gene            615..1922
                     /locus_tag="VIJ44_06040"
     CDS             615..1922
                     /locus_tag="VIJ44_06040"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY5165492.1"
                     /translation="MGNDGIVVTDQDWAEARSEAGTIARNAAGGGIVLVAVAYSVTGV
                     LLRVVSPGLGGLELVGVGTGVAAALSAPLLWWLARTAQAKGLETGSVRVAHDRVMRVQ
                     AKRREFETRLARALEMVVDEVAAFDVIQRAMVRMAPDAPVELLLADNSHAHLERMVTT
                     SPDGETVAGCTVDSPDQCEAARRAQTQVFRDSEELDACPLLRGRPQGRCSGVCVPVSI
                     MGRTVGVVHTTGVVDQPLTEPRVQALQTLANQAGNRIGMLRVMAETHLQASTDGLTGL
                     INRRSLENRIRLLRTEGVDFSFVMADLDHFKALNDANGHEAGDRALRIFAEALRHELR
                     TEDLACRYGGEEFAIVLPRSEAHEAIEAMGRVREGLAHATGRGDTPTFTASFGVAHSS
                     DAVDLDDLVQRADRALFAAKDAGRDRICLDGHSIPIARTLTAL"
     gene            complement(2075..2782)
                     /locus_tag="VIJ44_06045"
     CDS             complement(2075..2782)
                     /locus_tag="VIJ44_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165493.1"
                     /translation="MLVATVVRKTQPLMLRVGSAALAALAVVAWPSSAGAAPRASASP
                     AAILKAGLMVPRDVPSAWTSTTTPKNPELLRVEGIGACAATRAALNAASRGASRAFSR
                     KFVAPDQIGAAQDVVFVGRNDGAAQTFAGAYDGPQGQACVQAVVGKIAKQASGTAVVT
                     PLTDLAGLGDQALGYQFLISAPNHGAVVTGVYDLVVVRVGRAVTGFQFQNAILPLPER
                     TSIVSAVVARFRTVVTG"
     gene            3029..3229
                     /locus_tag="VIJ44_06050"
     CDS             3029..3229
                     /locus_tag="VIJ44_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165494.1"
                     /translation="MLVFAGALMFFVFVIGSELGGAVGQAALAFVFASAALVYLWRRL
                     DDATRRRFTLEARHWVRSSRRD"
     gene            3368..>4261
                     /locus_tag="VIJ44_06055"
     CDS             3368..>4261
                     /locus_tag="VIJ44_06055"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5165495.1"
                     /translation="MTVTLTKAAQVRERVDHPIIDADGHFVEIGPILQDELLASLEEF
                     GGAGLRDRFLASRTAPTDTASNLADRAEPSIREQWRAMPSWWGWPTHNARDRATAHLP
                     ALLYERLDELGIDFTILYPSMSLAFFEETDEELSSALCRAVNRFHARVFEPYRDRCTV
                     GALVPMNTPQQAVAELEFAVRELGMKSALIAGYARRPVGHDAYRLDMFGLDSEYDYDP
                     FWSACVELGVAPVVHSALQHHRVTRSISNYVYNHINGLAAAHESLCKSLFLAGVTRRF
                     PALRVGFLEGGVAWACSLFAGL"
BASE COUNT          642 a         1476 c         1468 g          675 t
ORIGIN      
        1 cttcgtctcc gacgaggcaa tcgaacccgt gacgttgacg ctgctgcctg gcgccgagtt
       61 ccaccagctg tgggcacgcg acgcgaccgc ctcgattccc gacgacacca cgacgacccc
      121 gcgccccgcc tacttcccgg ccgtgggcgg gttccggttc gggttcttca ccctcggccc
      181 cgagtcggtt gcctccccgg cggacatcga catcgaagcc gcgctcaacg agctgaccga
      241 gaagctgccc gggatggcgg aggccatgga gcccgagcac ccggggatgc acaccaccga
      301 cacgatcgac ttcgacatcg tcatctcggg tgaggtctgg ctcgagctcg acgacggcgc
      361 cgaggtccag ctgcgggccg gggattcggt ggtccagaac ggcacccgtc acgcctggca
      421 caaccgctcg tcacagccgt gcaccgtggc cgtcgccctc atcggcgctg ctcgcacccc
      481 cggctaggga gccgctcagc cacagggctg gtgtcccggg gctccacggc catctcgatt
      541 tctccgttca gggactcggt tcgcgcgccg atgtttgcct tcagatgtat gcgaatgggg
      601 cagaggaccg accaatgggc aacgacggaa tcgtggtgac cgaccaggac tgggccgagg
      661 cccggtccga ggccggcacg atcgcgagga acgccgccgg aggtgggatc gtcctcgtcg
      721 ctgtcgcgta ctccgtcacc ggggtcctcc tgcgggtggt ctcccccgga ctcggcggcc
      781 tggaactcgt cggggtcggc actggggtcg ccgcggcgct ctccgccccg cttctctggt
      841 ggttggctcg caccgcccag gcgaagggcc tcgagaccgg cagtgtccgg gtcgcccacg
      901 accgggtgat gcgggtccag gcgaaacggc gcgagttcga gactcggctg gcccgtgccc
      961 tcgagatggt cgtggacgag gtggccgcgt tcgatgtgat ccagcgggcc atggtccgca
     1021 tggctcccga cgctcccgtg gagctcctgc tcgccgacaa cagccacgct cacctggagc
     1081 ggatggtcac cacctcgccc gatggtgaga ccgtcgccgg ctgcaccgtc gactcccccg
     1141 atcagtgtga agcagctcgg cgagcccaga cgcaggtgtt tcgagacagc gaagaactcg
     1201 acgcctgccc cctgctgcgc ggacggccgc aaggccgctg ctcgggggtg tgcgtcccgg
     1261 tctcgatcat gggccgcacc gtcggagtgg ttcacaccac cggtgtcgtc gatcagccgc
     1321 tcacggagcc ccgcgtgcag gcgctgcaga cgctcgcgaa ccaggccggc aaccggatcg
     1381 ggatgctgcg cgtcatggcc gagacgcact tgcaagcgtc gaccgatggt ctgaccgggc
     1441 tcatcaaccg gcggagcctc gagaaccgga tccgtctcct gcgcaccgaa ggagtggact
     1501 tctcgttcgt catggccgac ctcgaccatt tcaaagcgct caacgacgcc aacgggcacg
     1561 aggccggcga ccgagccttg cggatcttcg ccgaggcgct gcggcacgaa ctccggaccg
     1621 aagatctcgc ctgccgctac ggcggtgaag agttcgcgat cgtgctgccc cgctccgagg
     1681 cgcacgaggc catcgaggcg atgggtcggg tccgggaggg gctcgcacac gccaccggtc
     1741 gtggcgacac gccgaccttc accgcgagct tcggcgtcgc ccactcgagc gacgccgttg
     1801 atctcgacga cctcgtgcag cgagcggacc gggcgctctt cgcggcgaag gacgcgggcc
     1861 gcgaccggat ctgcctggac gggcactcca tcccgatcgc ccgcaccctc accgcgctct
     1921 gatcccgacg tcggctcctg cgcaccgccg caccgcgggt gcgccacgtc cggctcggcc
     1981 cgagatgcgg gctcctagac cctcgaaccg ctgggcgggg aacctccggc gcctcccgac
     2041 ggatcgtcga gcgatcggtt tcgccggccg cgagtcagcc cgtcaccacc gtgcggaatc
     2101 gggcgacgac ggcgctgaca atgctcgtgc gctcagggag aggcaggatg gcgttctgga
     2161 actggaaccc ggtgacggcg cgaccaacac gaaccacgac caggtcgtag acgccggtga
     2221 ccacggcccc gtggttcgga gcgctgatca ggaactggta gccgagggcc tggtcgccca
     2281 acccggcgag gtcagtgaga ggcgtgacga ccgcggtccc ggaggcttgc ttcgcgatct
     2341 tgcccactac ggcctgaacg caggcctgac cctgcggccc gtcgtaagcc ccagcgaagg
     2401 tctgggccgc gccgtcgttc ctgccgacga agaccacgtc ctgggccgcc ccgatctggt
     2461 cgggcgcgac gaacttgcgg gagaaggcgc gactcgcacc tcgactggcg gcgttcaacg
     2521 ccgcgcgcgt cgcggcgcac gcgccgatcc cctcgacccg aagcagctca gggttcttcg
     2581 gagtggtggt gctcgtccag gctgacggga cgtcgcgcgg caccatgagc ccagccttca
     2641 ggatcgccgc gggcgacgcg ctggcccgcg gggccgcccc cgcgctggag ggccacgcca
     2701 cgaccgccag cgcggccaga gccgccgagc cgacgcgaag catcaacggc tgcgtcttcc
     2761 gaacgaccgt tgcgacgagc acgatcccgg acactatggg ccctgcggtg tgtgcgacga
     2821 agcctcggac acggctcggt ccggcggctg cgtctcggtc cgaagggagt cccctcgcgg
     2881 catccggtga gcggtgactt ggagcggcgc ggtgaggcca atgacccggt gcccgcgtac
     2941 gcttcaccgc atgagggctg acccccagag accgaatccg gatcgagggg ctgaaccgtc
     3001 gggtgtcgcg cgagcaggcc gcgggttcgt gctggtcttc gctggggcgc tgatgttctt
     3061 cgtgttcgtg atcggctcgg agctcggggg cgcggtgggc caggccgcgc tggcgttcgt
     3121 cttcgccagc gccgcgttgg tctatctgtg gcgccgcctc gatgatgcga cgcggcgccg
     3181 cttcaccctc gaggctcgcc attgggttcg atcgtcacgc cgagactgaa tctcagcccg
     3241 ctcggcccgg agaacgaagt cccagaaccg gccgctcgtc tgagcgtggc gcgtcgaggg
     3301 cgcggggaac ctcgacgcgg ttgaataggc gccgccagtc tcggacgggg ttgggaggag
     3361 gtcgggcgtg acagtcacgt tgacgaaagc ggcacaggtc cgtgagcgcg tcgatcaccc
     3421 gatcatcgac gccgatggtc atttcgtcga gatcggaccg attctccaag acgagctgct
     3481 ggcgtcactc gaggagtttg gcggcgcggg attgcgtgac cgattcctcg cgagtcggac
     3541 ggctccgacc gacacggctt cgaacctggc cgaccgagcg gaaccctcga ttcgggagca
     3601 gtggcgggct atgccgtcat ggtggggttg gccgacgcac aacgcccgtg atcgggcgac
     3661 cgcgcacctc ccggcactgt tgtacgagcg gctcgacgaa ctggggatcg acttcacgat
     3721 tctgtacccg tcgatgagct tggcgttctt cgaagagacc gacgaagagc tctcgtccgc
     3781 gctgtgtcgc gccgtgaacc gtttccacgc ccgcgtgttc gagccgtacc gtgaccgctg
     3841 caccgtcggt gccctcgtgc cgatgaacac gccgcaacag gcggtcgccg agctcgaatt
     3901 cgcggtgcgc gagctcggga tgaaatcggc gttgatcgcc ggctacgcgc gacgacccgt
     3961 cgggcacgac gcctaccgac ttgacatgtt cggtctcgac agcgagtacg actacgaccc
     4021 gttctggtcc gcgtgtgtcg agctgggggt cgcgcccgtg gttcacagtg cccttcagca
     4081 tcaccgtgtg acccggtcga tctccaacta cgtctacaac cacatcaacg gcctcgccgc
     4141 ggcccatgag tccctgtgca agtcgttgtt cctggccgga gtgacgcgcc ggttcccggc
     4201 gctgcgggtc gggttcctcg agggaggggt ggcttgggcg tgcagcctgt tcgcgggact
     4261 c
//
LOCUS       DATCCH010000318         4337 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_117566, whole genome shotgun sequence.
ACCESSION   DATCCH010000318 DATCCH010000000
VERSION     DATCCH010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4337
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117566"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="VIJ44_06060"
     CDS             <1..425
                     /locus_tag="VIJ44_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEY5165496.1"
                     /translation="GRRILSLAAREADIVSVNVTVTAGELGPGVAESATPRAFDEKMG
                     WIRDAAGDRFDSIELQCHCPFVMVTPDAASIAARLGSGFGLSAADALEVPLTLFGTVE
                     ELCDLVEARRERYGFSYWIVPDDAMDAFGPVVARLAGG"
     gene            complement(444..1454)
                     /locus_tag="VIJ44_06065"
     CDS             complement(444..1454)
                     /locus_tag="VIJ44_06065"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEY5165497.1"
                     /translation="MKIDGGLGGTGAEEVARVARELEAVGYDGGLTAETSHDPFLPLV
                     IAAEHTDRLQLGTGIAVAFARTPMTLANLSYDLHALSGGRFRLGLGSQIKAHIEKRFS
                     MPWSHPAPRMRELILAIRAIWAAWHEGSPLDFRGEFYHHTLMTPFFNPGPNPHGLPTI
                     QLAAVGQRMTEVAGEVADGIILHGFTTERYVREVTMPALERGFAKSGRARADFEISGP
                     LFVVTGTTDEELANAREGTKQQIAFYGSTPAYRGVLELHGWGELQAELNAASKRGEWK
                     EMGDLITDDILETFAVIAEPEDISKRLLERYGDLVDRISFYAPYRSDPDRWKGVLAGF
                     RS"
     gene            1546..2373
                     /locus_tag="VIJ44_06070"
     CDS             1546..2373
                     /locus_tag="VIJ44_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEY5165498.1"
                     /translation="MASVPYALSPSSIGSFKECPLAFKFAYVERLPEPPSVWTVKGTL
                     VHLALQYLLDRPADDRTLDAALADLDRARVALASDPECSALELDAEERATFDRESEQL
                     VRRYFDLEDPQTVHPIGLELRLEARVGGVRIRGVIDRLDLVDGELVVTDYKTGSVPSE
                     MFETKSLAGVHMYAALCEQTFGKRPERVQLLYLSRPEAIIATTSEQSCHGVARRTAAV
                     WTAVERACARDDFRPRPSRLCDFCTFRPYCPAHGGDPTDAAALRGPGTMIEPALPLG"
     gene            2419..2946
                     /locus_tag="VIJ44_06075"
     CDS             2419..2946
                     /locus_tag="VIJ44_06075"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY5165499.1"
                     /translation="MPGLAETVTAFDERVEKWLAPRRSAPLDHLMYGLSSAADHGLLW
                     LALGGARTARNGDLRWGGRFLAALAGESVVTNVVKLMFRRVRPQEHFENAERLPHGMR
                     RPITSSFPSGHAVTAFTAASVLSRDSRIGPVYYALAATIASSRVYTRIHHASDGLAGV
                     ALGLTLGRVARRLVR"
     gene            2967..3560
                     /locus_tag="VIJ44_06080"
     CDS             2967..3560
                     /locus_tag="VIJ44_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018500781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEY5165500.1"
                     /translation="MTRYISVTFDYRCPFARNANEAVIAALRSDALADVEWNFVPFSL
                     DQSHLEEGQVPVWAQEPAMWGSGVLALLYGIAVRDTFPDRFLEAHVALFAARHDQGRK
                     LDDEAVLRDVVASVGLDADAVAEEVWSGRPLKTLMTEHLQAVDECDVFGVPTFVEHDE
                     AVFVRFMDRGRVDDLERMVGLLDWTRLNEFKRTRLPR"
     gene            complement(3570..3932)
                     /locus_tag="VIJ44_06085"
     CDS             complement(3570..3932)
                     /locus_tag="VIJ44_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165501.1"
                     /translation="MPNPDDVLAILDEDGEELVLNLDEAARVLALTHDFDAATVSACP
                     GCRSRVLAAVAVVDLLSDEVDPSGRDLAELADDAPTLHLYVQDLATSCRHAAWHDPGF
                     SEWAEVLDELADSRRPVS"
     gene            3991..>4337
                     /locus_tag="VIJ44_06090"
     CDS             3991..>4337
                     /locus_tag="VIJ44_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEY5165502.1"
                     /translation="MHAVTVVEGTLVWAEHADPAPGSQELLVAVRAAGLNGADMLQRR
                     GLYPAPSGSPVDIPGMELAGEVLATGPGCTRFGPGDRVMAVVGGGGQAELAVVHERTA
                     LPVPDRLSWPEAGG"
BASE COUNT          596 a         1452 c         1572 g          717 t
ORIGIN      
        1 gtggccggcg catcctgtcg ctcgcggcgc gcgaggccga catcgtcagc gtcaacgtga
       61 ccgtcaccgc gggtgagctc gggcccgggg tggcggagtc ggcgacgccg cgcgccttcg
      121 acgagaagat gggctggatc cgcgacgcgg ccggcgaccg cttcgattcg atcgagttgc
      181 agtgccattg ccccttcgtg atggtcactc ccgacgcggc aagcatcgcg gctcggctgg
      241 ggtcggggtt tgggctctcc gccgcggatg cgctcgaggt tcccctgacg ttgttcggga
      301 ccgtggagga gctctgcgac ctggtcgagg cccgccggga gcggtacggc ttcagttact
      361 ggatcgtccc ggacgacgcg atggatgcct tcgggccggt ggtggctcgc ctcgccggag
      421 gttgagctcg gggacgggtt cggtcaggaa cggaacccgg cgagcacgcc tttccagcgg
      481 tccgggtcac tgcggtacgg cgcgtagaag ctgatccggt ccacgaggtc tccgtagcgc
      541 tcgagcagcc gcttcgagat gtcttcgggt tccgcgatga cggcgaaggt ctccaggatg
      601 tcgtccgtga tgaggtcgcc catctctttc cactcaccgc gcttggaggc tgcgttcagc
      661 tctgcttgga gttcgcccca gccgtggagc tcgaggacgc cgcggtacgc cggcgtggac
      721 ccgtagaagg cgatctgttg cttggtgcct tcgcgggcgt tcgcgagctc ctcgtcggtg
      781 gtgccggtca cgacgaacag gggtcccgag atctcgaagt cggctcgagc acggccgctc
      841 ttcgcgaacc cgcgttcgag cgcgggcatg gtgacctccc gcacgtagcg ctcggtcgtg
      901 aacccgtgga ggatgatgcc gtcggcgacc tcgccggcca cttccgtcat gcgctggccc
      961 accgccgcga gctggatcgt gggcagcccg tgcgggttgg gtccggggtt gaagaacggt
     1021 gtcatcaggg tgtggtggta gaactcgccg cggaagtcaa ggggtgaacc ctcgtgccag
     1081 gcggcccaga tcgcgcggat cgccaggatc agctcccgca tgcggggcgc gggatgggac
     1141 cacggcatcg agaaccgctt ctcgatgtgc gctttgatct ggctgccgag accgagacgg
     1201 aaccggccgc cggacagggc gtggaggtcg tagctgaggt tggcgagcgt catgggcgtc
     1261 cgggcgaacg cgacggcgat cccggtgccg agctggagcc ggtcggtgtg ttcggcggcg
     1321 atcaccagcg gcaagaacgg gtcgtggctc gtctcggcgg tgaggccgcc gtcgtagccg
     1381 accgcctcga gctcccgggc gacgcgggcg acttcttcgg ctccggtacc cccgagccct
     1441 ccgtcgatct tcatgaccgc ggagcgtatc ggcaccgcgc gggaccgggt cgggggtccg
     1501 gcgggtccgt cgccccgtca caccctggcg ataccctccg ctctagtggc gtcggtgccg
     1561 tacgcgttgt ccccgagctc gatcgggtcc ttcaaggagt gccccctcgc gttcaagttc
     1621 gcgtacgtcg agcggctccc cgaacccccg tcggtatgga cggtcaaggg gacgctggtg
     1681 cacctggcgt tgcagtatct cctcgaccgt cccgccgacg accggacgct cgacgccgcg
     1741 ctcgctgacc tcgaccgggc ccgcgttgct ctggcctccg acccggagtg ctccgcgctc
     1801 gagctcgacg cggaggagcg ggccacgttc gaccgcgaat ccgagcagct ggtgcggcga
     1861 tacttcgacc tcgaagaccc tcagacggtc cacccgatcg ggttggagct tcgactcgag
     1921 gctcgcgtcg gcggtgttcg aatccgcggg gtcatcgacc gcctcgacct cgtcgacggg
     1981 gagctggtcg tgaccgacta caagaccggg tcggtgccct cggagatgtt cgaaacgaag
     2041 agcctcgccg gggtgcacat gtacgcggcg ttgtgtgagc agacgttcgg taagcgtccc
     2101 gaacgggtac agctcctcta cttgtcgcgg cccgaggcga tcatcgccac gacgagtgag
     2161 cagtcctgtc acggcgtcgc tcgacgtacc gcagcagtct ggacggcggt cgagcgggcc
     2221 tgcgcccgtg acgacttccg gccccgcccc agtcggctct gtgacttctg cacgttccgg
     2281 ccgtactgcc ccgcgcacgg cggcgacccg acggacgccg ccgcgttgcg cggtcccggg
     2341 acgatgatcg aaccggccct cccgctcggc taaccgcggc gacggcagac gcgaacctca
     2401 ccggtatgct cggccgcgat gcctggtctg gctgagacgg tcaccgcgtt cgacgagcgc
     2461 gtcgagaagt ggttggcgcc gcgccgttcg gcaccgctcg atcacctcat gtacgggctc
     2521 tcgagcgcgg ccgatcacgg tttgttgtgg ctcgccctcg gcggggctcg gaccgcacgc
     2581 aacggcgacc ttcgctgggg cgggcggttc cttgccgcgc tcgcgggtga gtccgtggtc
     2641 acgaacgtgg tgaagctcat gttccggcga gtgcgacccc aggagcactt cgagaacgcc
     2701 gaacgtctcc cgcacgggat gcgacgcccg atcacgtcgt cgttcccatc cggacacgcg
     2761 gtcacggcgt tcaccgcggc gtcggtgctg tcccgcgaca gcaggatcgg gccggtgtac
     2821 tacgcgttgg cggcgacgat tgcctccagc cgggtgtaca cgcgcatcca tcacgcatcg
     2881 gatgggctgg ccggcgtcgc actcggactc accctcgggc gtgtcgcgag gcgactggta
     2941 cggtgaacct tgaaccagga gacgccgtga cccgctacat ctcggtcacc ttcgactacc
     3001 gttgcccgtt cgcccgcaac gcgaacgaag ccgtcatcgc cgcgctgcgg tccgacgccc
     3061 tcgccgacgt cgaatggaac ttcgttccgt tctcgttgga tcagtcccac ctcgaggaag
     3121 gccaggttcc cgtctgggcg caggagccgg cgatgtgggg gtccggcgtc ttggcgctgc
     3181 tgtacgggat cgcagttcgc gacacgtttc ctgaccgttt cctggaggcg cacgtcgcgc
     3241 tgttcgcggc ccgccacgac caggggcgca agctggacga cgaagcggtg ttgcgagacg
     3301 tcgtcgcctc ggtcggtctt gacgccgacg cggtggcgga ggaagtgtgg agcggccggc
     3361 cgttgaagac gttgatgacc gagcatctgc aggcggtcga cgagtgcgac gtctttggtg
     3421 tcccgacctt cgtggagcac gacgaggccg tcttcgtgcg gttcatggac cgcggccggg
     3481 tcgacgacct ggaacgcatg gtggggctgc tcgactggac ccgcttgaac gagttcaagc
     3541 gcacccgact cccccgctga cgaccagcct cagctgacgg ggcgtcggga gtcggccagc
     3601 tcgtcgagga cctcagccca ctccgagaac ccggggtcgt gccaggcggc gtggcgacag
     3661 gaggtcgcca ggtcctggac gtagagatgc agggtcggcg cgtcgtcggc cagctcggcg
     3721 aggtcacggc cgctcgggtc gacctcgtcg ctcaggagat cgaccacggc gaccgcggcg
     3781 aggacccggc tgcgacagcc gggacaggcc gagacggtgg ctgcgtcgaa gtcgtgggtg
     3841 agggcgagga cccgggcagc ctcgtcgagg ttgagcacga gctcctcccc gtcttcgtcg
     3901 aggatcgcca gcacgtcgtc ggggttcggc actcgacagg acggtaccgc ccgggcgcga
     3961 cgccaccggc ggccctacgc tccccgggtc gtgcatgccg tgaccgtcgt cgaggggacc
     4021 ctggtctggg ccgagcacgc cgatccggcc ccgggcagcc aggagctcct cgtggcggtg
     4081 cgggccgcgg gcctcaacgg cgccgacatg ttgcagcgcc gagggctcta cccggccccg
     4141 tcgggatcgc cggtggacat cccgggcatg gagctcgcgg gcgaggtgct cgcgaccggc
     4201 cccgggtgca cccggttcgg gcccggcgac cgagtcatgg ccgtcgtcgg cggcggcggg
     4261 caagccgaac tcgcggtggt ccatgagcgg acggcgctcc cggtgcccga tcgcctgtcc
     4321 tggcccgagg cgggagg
//
LOCUS       DATCCH010000319         3016 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_58164, whole genome shotgun sequence.
ACCESSION   DATCCH010000319 DATCCH010000000
VERSION     DATCCH010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58164"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            247..972
                     /locus_tag="VIJ44_06095"
     CDS             247..972
                     /locus_tag="VIJ44_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018542523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY5165503.1"
                     /translation="METRVDEIADGIFRLSTLVPDIAPPAGFSFNQFLITGDEPLLFH
                     AGPRSMFPLVSQALATVIPPKSLRWITFGHVEADECGAMNDWLAAAPQALVAHGAVGC
                     MVSLNDLADRPPRALADGEVIDLGGKRVRHIDTPHVPHAWEARLLYEETTGTLLCGDL
                     FTHVGDGPALTEQDIIGPAIATEEMFRYSSLGPTTTSVMHSLADLSPKRLALMHGSSY
                     SGDGGTALRELATAYEERFLTPA"
     gene            complement(1103..1744)
                     /locus_tag="VIJ44_06100"
     CDS             complement(1103..1744)
                     /locus_tag="VIJ44_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012949206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY5165504.1"
                     /translation="MDDRHRGFPDGFFERTDPSPDARFYAPPRLVTHIDEGAVAAVGA
                     LYDELGLSGEVLDLCSSWVSHFRVAPARLVVLGMNPVELDQNPQAAARVVHDLNRDPQ
                     LPFADASFDAATCCVSVDYLVHPIEVFDEVARVLRPQARFVCTFSNRCFPTKAIRGWL
                     ATDDPSHLVIVRDYFHRSNGWGDVVAERRTPPNHRGDPLFAVWADRDGASIAD"
     gene            complement(2232..2651)
                     /locus_tag="VIJ44_06105"
     CDS             complement(2232..2651)
                     /locus_tag="VIJ44_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165505.1"
                     /translation="MVVSRLLLMSAFGLGLSVPGASSVPQPHTVSDLAATISAKGLGC
                     QDLQVAPPPSETIEATCTVWHEANVELDVLSSHAVLTKQVPKAVATVCAQLRKAHSSV
                     KIVFVIGSNWVATFESTVNARPLAKAMNAKIQPLKCS"
BASE COUNT          545 a          985 c          993 g          493 t
ORIGIN      
        1 ttgggggtca ttgtgtcgga tcgagcgccc cagctccacc gcgcacactc atgaagatcg
       61 cctaggcccg acactgcgtc tggccgtcct ggaacaacga accgcgatcg ttgaggcgag
      121 tggccccagc atcggtggcg accctgagcg cgccgtggcg tcgcacgacg ccggccgcgc
      181 cgcttgcgac gtatggtgcg acgagatccg agcgatcgcg ctcggcggtc gacaggagtg
      241 gcgatgttgg agacacgggt cgacgagatc gccgatggga tcttccgatt gtcgacactc
      301 gtcccggata tcgctcctcc agccgggttc tcgttcaacc agttcctgat caccggagac
      361 gaaccgctct tgttccatgc tggcccgcga tccatgttcc cgctggtttc gcaggcattg
      421 gccacggtga ttccgccgaa atcgctcagg tggatcacat tcgggcatgt ggaggccgac
      481 gagtgcggcg cgatgaacga ctggctcgca gcagcccccc aagccctggt ggcacacggg
      541 gccgtcggct gcatggtgtc gttgaacgac ctagccgacc gacccccacg ggctctggcc
      601 gacggtgagg tcatcgacct cggcggcaag cgggtccgcc acatcgacac gccgcacgtc
      661 ccccacgcgt gggaggcgag actgctctac gaggagacca cgggcacgct gctgtgcggg
      721 gacctgttca cccacgtcgg agatggcccg gcgctcaccg aacaagacat catcggccca
      781 gccatcgcca ctgaggagat gttccggtac agcagcctcg gccccaccac gacgtccgtg
      841 atgcattcct tagctgacct ttcccccaag cgtctcgccc tgatgcacgg ttcttcgtac
      901 tccggcgacg gtgggacggc acttcgcgag ctcgcgaccg cgtacgaaga acgtttcctt
      961 actcccgcgt agtcatagtg cgggctcgag ggtgcgcccc tcgaggcatc caatccgtca
     1021 cttcaggtcc cgcgactgcg ccgtgactcg cgatcgttca gatcgcgagt cataagtgac
     1081 gcaccgcgcc tgccaggcct actcagtcgg cgatagaagc gccatcacgg tccgcccata
     1141 ctgcgaacag cggatccccg cggtggtttg gtggggtgcg acgctcagca acgacatcac
     1201 cccagccgtt tgagcggtgg aaatagtccc gcacgatgac gagatgggat gggtcgtcgg
     1261 tcgcgagcca gccgcggatc gccttcgttg ggaagcaccg gttggagaag gtgcagacga
     1321 accgagcctg gggtcgtagg acgcgggcaa cttcgtcaaa gacctcgatt gggtgcacga
     1381 gatagtcgac ggagacgcag caggtcgcgg cgtcgaatga tgcgtcggcg aatgggagct
     1441 gggggtcccg gttgaggtcg tggacgacac gagccgcggc ctgaggattc tggtcgagtt
     1501 cgacgggatt catgccgagg acgaccaagc gagccggggc gacacggaag tgcgacaccc
     1561 acgatgagca gaggtcgagc acctcacccg agaggccgag ttcgtcgtag agggcaccga
     1621 cggcagcgac ggcgccctcg tcgatgtggg tcacgagtcg gggcggcgca tagaagcgag
     1681 cgtcgggcga agggtcggtg cgttcgaaga agccgtcagg gaacccgcgg tgccggtcat
     1741 ccacccgccc atgatcgcgg acctgcgtgg cccgcttccg gttggtgatt cgcggttccg
     1801 tagaccgcga atcgcctccg aggtctcgcg tataggtgca agcctgatcc gcaatcgacg
     1861 acacgacggg tagacggccg acggcctcac cgtcctaccc cacgccgacc ttctgaagta
     1921 tgacgtcgac ggagagtcgg gagccaccgg accacgtctc gcaccgcctt gaggaagcgc
     1981 ttgagcgccg acgcggcaaa gaacgccatt gccgctaagg ccgcaccccg cacgggtgac
     2041 acgcggaacg gtcgccgaca gctccccgct gccgtgaggc tcgttccgga gccgagaact
     2101 ggagttggga ggcctttgcc ggtcgagccc caatcgttac ccgagtcgcc tccgagcgcc
     2161 agatcccgcc aacgcgtccc cgacgcaccg acgctggggg aaccgagcga ggttctcgac
     2221 cgggcctttc cctagctgca cttcagcggt tggatcttcg cgttcatcgc cttggccagc
     2281 gggcgggcat tcaccgtgct ctcaaacgtc gccacccagt tggacccgat gacgaacacg
     2341 atcttcaccg aggagtgcgc tttgcgcagc tgcgcacaca ccgtcgccac cgccttcgga
     2401 acttgtttcg tgaggacggc gtgacttgag agaacgtcga gttcgacgtt cgcctcgtgc
     2461 caaaccgtac aagtggcctc gatcgtctcc gacgggggcg gcgccacctg aagatcctga
     2521 catccgaggc ccttcgccga gatcgtcgcg gccaagtcgc tgaccgtgtg aggctgcggg
     2581 accgacgacg cgccaggaac cgagagccca agtccgaagg ctgacatgag cagtaggcgc
     2641 gagacgacca cggagatctc ctccacacgg gaagccggac gggtgttccg ctcgccccag
     2701 atcgaacact ggtcgagtgg cgaaccccaa cctatcgtcg gagcgaaaga gttcggggcg
     2761 tagccaacga gcctcgcttt cgtactccag gagccgacct catgccgatt ggagcatgca
     2821 ggccgctcgg ccctcgcatg agcggcgggg cgtgacgtct tagggtgcag agcatgacac
     2881 cgagagcttg gccgcttcgc tgctcgggga tcaccccgcc gtccacacca tgaagggcat
     2941 tgccgccatc ctgggcgcgg ttgcggttgt cgcgaccgca ggagcgggcg gcggcctctc
     3001 gccgatcggt gcgaga
//
LOCUS       DATCCH010000320         2054 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_224491, whole genome shotgun sequence.
ACCESSION   DATCCH010000320 DATCCH010000000
VERSION     DATCCH010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224491"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1020)
                     /locus_tag="VIJ44_06110"
     CDS             complement(<1..1020)
                     /locus_tag="VIJ44_06110"
                     /inference="COORDINATES: protein motif:HMM:NF017722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004062 - aryl sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HEY5165506.1"
                     /translation="MLLVVVGAGCVPSAPPPPPTTTTALPSPPMLDVSANPGLFPAFD
                     PSVSDYVIRCTGAPVEVTIGAPAGTTVSIDGQPAASGRFTDSVSRSTGQRFTLVTRRR
                     TGGGRPGPEATSTYSMRCLPTDFPAWTATRTGTTQAEYYATVPISGTGSGPGAGYPAI
                     FDNAGVPVWWGPKTNTPFAELLPDDNIAWTKSDGTPAEERRLDGTLVRTVTTSVGVPN
                     QHDLLRLANGDYLMAADVVRTGIDLTSAWGPAYPSDASVLDAVIQELTPAGAVVWSWD
                     AMDHIPVSETDPQWRDQVMNGSYDAYHWNSIDSTGTGFIMSFRHLDAVFNIDQRSGKI
                     LWKLGG"
     gene            complement(1563..>2054)
                     /locus_tag="VIJ44_06115"
     CDS             complement(1563..>2054)
                     /locus_tag="VIJ44_06115"
                     /inference="COORDINATES: protein motif:HMM:NF022732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3089 domain-containing protein"
                     /protein_id="HEY5165507.1"
                     /translation="GGDFQHIPACRSRTQVGCVVAFSTFDEPPPPNTKFGHTSTPGLA
                     VLCTNPAALPGGSGSLDPIFPTQPFAPGSTIATGIRLLGVTLPSATTPWVESSGSYTA
                     RCSSTNGANVLEIAPVDGAPNIHPSPDSTWGLHLVDANIALGNLTALVQSEATAYARG
                     TAR"
BASE COUNT          328 a          666 c          740 g          320 t
ORIGIN      
        1 cccgccgagc ttccagagga tcttcccgct gcgctggtcg atgttaaaga cggcatcgag
       61 gtggcggaac gacatgatga acccggtccc cgtcgagtcg atcgagttcc agtggtacgc
      121 gtcgtagctt ccgttcatga cctggtcgcg ccactgcgga tcggtctcag aaaccgggat
      181 gtggtccatt gcgtcccatg accacacgac cgcgccggcc ggcgtgagct cctggatgac
      241 tgcgtcgagc accgaggcgt ccgacggata cgcgggcccc catgccgacg tgagatcgat
      301 accggtccgc acgacgtcgg cagccatgag gtagtcgccg ttggcgagcc ggagcaggtc
      361 gtgctggttg ggcacgccca ccgatgtcgt gacggtacgc accaacgtgc cgtccagacg
      421 ccgctcctcg gcgggggtgc catcggactt cgtccaggcg atgttgtcgt caggcaggag
      481 ctcggcgaac ggggtattgg tcttcggccc ccaccagacg ggcacgccag cgttgtcaaa
      541 gatggccggg tagcccgccc cgggtcctga cccggttccc gagatcggca cggtggcgta
      601 gtactccgcc tgcgtggtcc cagtacgggt cgcggtccag gcgggaaagt ccgttgggag
      661 gcagcgcatg gagtaggtgc tcgtcgcctc gggtcccggt cgacctccac cggtgcgtcg
      721 acgggtcacg agcgtgaagc gttgaccagt gctacgactc acgctgtcgg tgaagcgccc
      781 gctcgcagcc ggttgtccgt cgatggacac ggtcgtgccc gcaggcgcgc cgatggtgac
      841 ttcgacgggg gcaccggtgc agcggatgac gtagtcgctg acgctcgggt cgaacgccgg
      901 gaacagcccc gggttggcgg agacgtccaa catcggaggc gacggcagcg cggtcgtggt
      961 cgtcggaggc gggggtgggg cactgggcac gcagccggcc ccgacgacca cgagcagcac
     1021 gccgagcatg acagcgccac gccatccccc ccacgcagac cgatcaccgt cgggcccgcg
     1081 ggtgcgacgg gtcgaaccac gacgcaaccc aacggctgcg ctgacgggcc gggcaaacac
     1141 gacgatggca gccatcgaga caggggctcc tcagtggaag gaggacgccc gcaccgtccg
     1201 caagtcgttt cggtgagaca cccttgtcgg ggctcgtggc gaaatcaagt cacctcaacg
     1261 gcgagttcgg gagcatcgcg tcgacgccga ccacgttggg gacggtgacg gtgggcagca
     1321 ccggtacgtt cacccagtcg ttcacggtgc cgtgccgcgt gctcgccggg gaccataccg
     1381 tcagcgctac cgcgccgttc ccttctttcc ggctcccagc ctcctgaaca gtgctgaaac
     1441 accggagacg ccgcgcgtcg cgccggggct tgctcgcgcc gagctcgctg agcagatcag
     1501 tcgacatggc agtgtcgcgc cacgcggctg agcgcgggag tcggctcagg agcctgactt
     1561 gactagcgag ccgtgccgcg ggcgtacgcg gtggcctccg actgcaccag cgcggtcaga
     1621 ttccccagcg cgatgttcgc gtcgacgagg tgcagtcccc aggtcgagtc cggggacggg
     1681 tggatgttcg gcgcgccatc caccggtgcg atttccagga cgttcgcccc gttggtcgag
     1741 gagcagcgcg cggtatagct gcccgacgac tcgacccagg gtgtggtcgc gctgggcagg
     1801 gttacgccga ggagtcggat ccccgttgcg atggtcgatc caggggcgaa cggctgggtc
     1861 gggaagatcg ggtcgaggct gcccgagcca cccgggagtg ccgcggggtt cgtgcacagc
     1921 accgccagcc ctggtgtgct cgtgtgccca aacttagtgt tcggcggcgg gggttcgtcg
     1981 aaggtcgaga aggcgaccac acagccgacc tgggtgcgcg aacggcaggc cgggatgtgc
     2041 tggaagtcgc cgcc
//
LOCUS       DATCCH010000321         3715 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_391098, whole genome shotgun sequence.
ACCESSION   DATCCH010000321 DATCCH010000000
VERSION     DATCCH010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391098"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VIJ44_06120"
     CDS             <1..294
                     /locus_tag="VIJ44_06120"
                     /inference="COORDINATES: protein motif:HMM:NF015113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HEY5165508.1"
                     /translation="VSVLPVADRHHAYGFRIADRLRAEGYRVELLDAHDDTLGARVRR
                     AKLEKVPYVLVVGDDDAADGTVGVNARGTDQPERGVAVDGFVTRLGAEVAERR"
     gene            291..788
                     /locus_tag="VIJ44_06125"
     CDS             291..788
                     /locus_tag="VIJ44_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEY5165509.1"
                     /translation="MTLERLWAGWRASYIEDVANVEPSNDTCLFCALGSATGDDGQVV
                     AQAPLTFAVLNLYPYTSGHLMVVPHRHVGDLEALTTDEADALMAMTRQAIVALKAAYQ
                     PDGVNVGMNLGRAAGAGVPGHLHVHVVPRWLGDTNFMTSVAESRVLPEPLARSFERLR
                     AEWPT"
     gene            799..1272
                     /locus_tag="VIJ44_06130"
     CDS             799..1272
                     /locus_tag="VIJ44_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165510.1"
                     /translation="MTEPGDALPAELDVTAYVGPYLFPTPRRRLVAAGCLLAVAVAAL
                     AGYAASGNRGLLVGAGTLVTAAAYHAVTAWPLTIDQTEGLVLATRAVGFPVGHASAQL
                     TWRGLRSRPTWRILLYSADTPPSMRGLVELDAVDGTVLGEYTETNPEDWSVYTAS"
     gene            complement(1279..3291)
                     /locus_tag="VIJ44_06135"
     CDS             complement(1279..3291)
                     /locus_tag="VIJ44_06135"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEY5165511.1"
                     /translation="MKTVPSPMIRNVALVGHGHAGKTTLTEALLFVGGAISRMGRVED
                     GNTVSDFDAEEQKRTISVSLTVAPFEWEGHKVNVLDTPGYADFITDVAAALRVADLAV
                     FVVSAVEGVEVQTEALWRLAEARGMPRTIFVNKLDRERASFTRTLDELKDRFGTGVAP
                     LELPIGEEAAFHGVVDLLTDTAVVYDGETGRGVAAPVPDDMATEEHSVHDALIEGIVV
                     ADDDLMERYLADETLPVEELEHALATGIADATVFPVLCGSATRLIGVDRLARFIAEDG
                     PAPMVSDGPPAAFVFKTVVDPYIGRVNLFRVLQGSVDADATLVNDRTLSDERLHQLVA
                     LRGKEQEPVKSVGGGDLAAVAKLSDTTTGDVLGARGADIDVERLERPEPVLPVAIRAK
                     SKGDEDKLANALHRLQDEDPAVRIERNAETHQTLLWGMGETHLGIALERLQRKFGVAV
                     ETDDVKVAYRETITNTAEAEGRYKKQTGGHGQFGVAFLRVEPRERSSGFEFKDAVVGG
                     AIPRQFIPAVEKGIAEAMQSGGVYGYPVVDVAVTCFDGKYHSVDSSEMSFKMAGSIGF
                     KEAMAKAAPILLEPISELVVTAPEHSLGDIMGDLNSKRGRIQGSGSVGNGEVEIVALV
                     PTSEVLRYAIDLRSITGGRGRFAMQHSHYDPVPTQLASKLVPAAAS"
     gene            3463..>3715
                     /locus_tag="VIJ44_06140"
     CDS             3463..>3715
                     /locus_tag="VIJ44_06140"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEY5165512.1"
                     /translation="MLDRRWREKAEQALEPVGRALHVVGVSADGLTVFGLFAAAGTAA
                     LIANGNLLLAVFGLILTGLPDLLDGSVARQSGTAGRRGAF"
BASE COUNT          526 a         1395 c         1207 g          587 t
ORIGIN      
        1 gtctcggtgc tccccgtcgc ggaccgccat cacgcgtacg ggttccggat cgcggatcgg
       61 ctccgcgccg agggctaccg agtcgagctt ctcgacgccc acgacgacac cctcggagcc
      121 cgggtccggc gcgccaagct cgagaaggtc ccgtacgtcc tcgtcgtcgg cgacgacgac
      181 gccgccgacg ggaccgtcgg cgtcaacgcc cggggaaccg accagcctga acggggcgtc
      241 gcggtcgacg ggttcgtgac ccgactcggc gcggaggtcg cggagcgccg gtgaccctcg
      301 agcggctctg ggccggctgg cgcgcgtcgt acatcgagga cgtcgccaac gtggagccgt
      361 ccaacgacac ctgcctcttc tgcgccctcg gctcggcgac cggtgacgac ggccaggtcg
      421 tcgcccaggc tccgctgacg ttcgcggtgc tgaacctcta cccgtacacg tcgggccatc
      481 tcatggtcgt accccaccgc catgtggggg acctcgaggc gctcaccacc gacgaagccg
      541 acgcgctcat ggccatgacc cgccaggcga tcgtggcgct caaggccgcg tatcaacccg
      601 acggcgtgaa cgtcggcatg aacctggggc gggccgcggg tgccggcgtc cccggtcacc
      661 tccacgtcca cgtcgtgccc cgctggctgg gtgacacgaa cttcatgacc agcgtggcgg
      721 aatcccgcgt cctacccgag ccgctcgccc gttccttcga gcgcctccga gccgagtggc
      781 ccacgtaagg tcgatggcgt gaccgagccc ggcgacgccc tccccgccga gctcgatgtc
      841 accgcgtacg tcgggcccta cctgttcccg acaccccggc gtcggctcgt cgccgccggc
      901 tgcctcctcg ccgtcgcggt ggcggccctg gccgggtatg ccgcaagcgg taaccggggg
      961 ctgctcgtcg gcgccgggac cctcgtcacc gccgccgcgt accacgcggt gaccgcctgg
     1021 ccgctcacca tcgatcaaac cgaaggtctc gtgctcgcga cccgcgccgt cggcttcccc
     1081 gtcgggcacg cctcggcgca gctcacctgg cggggcttgc gctcccgccc gacgtggcgg
     1141 atcctgctct acagcgccga cacgccccca tcgatgcggg gcctcgtcga gctcgacgcc
     1201 gtcgacggga cggtcctcgg cgagtacacc gagaccaacc ccgaggactg gtcggtctac
     1261 accgcgtcgt aacgagggct aggacgccgc ggcggggacg agcttcgacg ccagctgcgt
     1321 cggcaccggg tcgtaatgcg agtgctgcat cgcgaaccgc cctcgccccc cggtgattga
     1381 gcgcaggtcg atcgcgtagc gcagaacctc cgacgtcggg accagcgcca cgatctccac
     1441 ttccccgttc ccgacgctgc ccgagccctg gatgcggccc cgcttcgagt tcaggtctcc
     1501 catgatgtcg ccgagcgagt gctcgggtgc ggtgacgacg agttcgctga tcggctcgag
     1561 cagaattggg gccgctttcg ccatcgcctc cttgaacccg atggaacccg ccatcttgaa
     1621 gctcatctcc gacgagtcaa cagagtggta cttgccgtcg aaacaggtca ccgccacgtc
     1681 aaccaccgga tatccgtaca caccacccga ctgcatcgcc tccgcgatgc ccttttccac
     1741 cgcggggatg aactgacggg ggatcgcccc gccgacgacg gcgtccttga actcgaagcc
     1801 cgagcttcgc tcccggggtt ctacccgaag gaacgccacc ccgaactggc cgtgaccacc
     1861 ggtctgtttc ttgtaccggc cctcggcctc cgcggtgttg gtgatcgtct ctcggtacgc
     1921 gaccttcacg tcatcggttt cgaccgcgac cccgaacttg cgttgcagcc gctccagcgc
     1981 gatcccgaga tgggtctcac ccattcccca caacagcgtc tggtgggtct cggcgttgcg
     2041 ttcgatccgc accgccgggt cttcgtcttg gaggcggtgc agcgcgttcg ccagcttgtc
     2101 ctcgtcgcct ttggatttcg cccggatcgc gaccggcagg accggctcgg gtcgctcgag
     2161 gcgctcaacg tcgatgtcag cgccgcgggc cccgagcacg tcccccgtgg tcgtgtcgct
     2221 gagcttcgcg accgcggcga ggtcgccgcc gccgaccgat ttcacgggct cctgctcctt
     2281 gccccgtaac gccacgagct ggtggaggcg ctcatcggac aaggtgcggt cgttcaccag
     2341 agtcgcgtcg gcgtcgacgc tgccctgcag cacccggaac aggttgacgc gcccgatata
     2401 cgggtcgacg acggtcttga acacgaaggc cgccggtggt ccgtcggaga ccattggtgc
     2461 tggtccatcc tcggcgatga accgggcgag gcgatcgacg ccgatcagcc tcgtcgcgct
     2521 gccgcacagg acggggaaca ccgtcgcgtc ggcgatacca gtcgcgaggg catgctccag
     2581 ctcctcgacg ggcagggtct cgtcggcgag gtagcgctcc atgaggtcgt cgtcggcgac
     2641 gacgatgccc tcgatcagcg cgtcgtggac cgagtgctcc tcggtcgcca tgtcgtccgg
     2701 aaccggagcg gcgacgccgc gcccggtctc gccgtcgtag acgacggccg tgtcggtgag
     2761 gaggtccacg acgccatgga acgcggcttc ctcaccgatc gggagctcca ggggcgcgac
     2821 gccggtcccg aaccggtcct tgagctcgtc gagggtccgg gtgaacgagg cccgctcccg
     2881 gtccaacttg ttcacgaaga tggtgcgggg catcccgcgc gcctcggcga ggcgccacaa
     2941 cgcctcggtt tgcacctcga cgccttcgac ggccgagacg acgaacaccg ccaggtcggc
     3001 gacccgcagt gccgccgcga cgtcggtgat gaaatcggcg taccccgggg tgtcgagcac
     3061 gttgaccttg tggccctccc attcgaaggg cgccacggtg agcgacaccg agatggtgcg
     3121 cttctgctcc tcggcatcga agtcgctgac ggtgttgccg tcctcgaccc ggcccatgcg
     3181 gctgatcgcg ccgcccacga acagcagcgc ctcggtcaga gtcgtcttcc ccgcgtgccc
     3241 gtgccccacc aacgcgacgt tgcggatcat cggcgacgga acggtcttca cgctcgcacc
     3301 ccctgcgctg acgtgccggg gatcgtaccg gtgctcccgc ccggtgatcc ccgatccggg
     3361 agcctcctgg taccctccga acggttcctg cggaacgtcc gggcacgccc caccgggcgc
     3421 cgcccccaga cgtgccccgc aaccgggaag gacccgcggc ccatgctcga ccgacgatgg
     3481 cgcgagaagg ccgaacaggc cctggagccc gtcggccggg ccctccacgt cgtcggtgtc
     3541 tccgccgacg gactcaccgt cttcggcctg ttcgccgccg ccggcaccgc ggcgctgatc
     3601 gccaacggga acctcctcct cgcggtcttc ggcctgatcc tcaccggtct ccccgacctc
     3661 ctcgacgggt cggtcgcccg ccagagcggc accgcgggac gccgcggcgc gttct
//
LOCUS       DATCCH010000322         2264 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_491927, whole genome shotgun sequence.
ACCESSION   DATCCH010000322 DATCCH010000000
VERSION     DATCCH010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491927"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1240
                     /locus_tag="VIJ44_06145"
     CDS             <1..1240
                     /locus_tag="VIJ44_06145"
                     /inference="COORDINATES: protein motif:HMM:NF023401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165513.1"
                     /translation="MVELVERIAELAGIGITARSTTDQRESLEGADFVVVNISTGGFE
                     SMRHDLEIPARYGLRQSVGDTVGPGGIFRALRNIPVFSGIARDMEELCPDAWMLNLTN
                     PMTAICRGVTKVSSIKTIGLCHEITLAQFILSLLLDRSFLELTATVAGVNHLPVITAL
                     DVGGEDGLALLRDLLDHESERGDEPVSTEFVEGLGHEKVSEGGEWTKRDLLHANRVKL
                     ELFRRFGVLPGAGDRHLVEFFAGFLTEESRWGERWGVGLTSIEDRERGQADHIRDFHQ
                     LVAMTEVDDMPSGEMVAPVMMCLLEDQPGWFPLNIPNQGQVADVADDAVVESMCVVDG
                     SGVRGRDVVHVPPVLADTLRRVSTAQELTVDAALTGDREQVFEAMMVDPLAGRLDYDT
                     LRDLTDEMLVATKRWLPQFA"
     gene            1243..2010
                     /locus_tag="VIJ44_06150"
     CDS             1243..2010
                     /locus_tag="VIJ44_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007355605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEY5165514.1"
                     /translation="MGEAFTVDAMTVQVHDSDTELASDAAAEAAEAIGAAIGARGVAN
                     VMFATGNSQLGFLEALTARDDVDWDRLVGFHMDEYRGVSAEHPASFRRYLRERIVDRV
                     HPGEFQFIAGDAADPAGECARYAALLRDHPLDLCCLGIGENGHLAFNDPPVADFDDRV
                     DVKVVALDEACRRQQVGEGHFATVPDVPAEAITVTIPALLRAGRVLAIVPDARKRDPV
                     RVALTGPVTTVCPASILRTRSNVTLHLDRSSAADVAP"
     gene            2007..>2264
                     /locus_tag="VIJ44_06155"
     CDS             2007..>2264
                     /locus_tag="VIJ44_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165515.1"
                     /translation="MTAPELHVVTFPSLAELRRRLPRLLAGIVVLSAGITLMLQARLG
                     VSAYDVLHQGLSKVTGLSFGTVVVLLGLVILVGWIPIGQRFG"
BASE COUNT          322 a          718 c          854 g          370 t
ORIGIN      
        1 gatggtggag ctcgtcgagc gcatcgccga gctggcggga atcgggatca cggcccgaag
       61 cacgaccgac cagcgtgagt ccctggaagg tgccgacttc gtcgtggtga acatctccac
      121 tggcgggttc gagtcgatgc gccatgacct ggagatcccg gctcggtacg ggttgcgcca
      181 gtcggtgggg gacaccgtgg gtccgggcgg gatcttccgg gcgttgcgca acatcccggt
      241 gttcagcggg atcgcccgcg acatggagga gctgtgcccc gacgcgtgga tgctgaacct
      301 caccaatccg atgacggcga tctgtcgcgg ggtgacgaag gtgtcgagca tcaagacgat
      361 cgggttgtgt cacgagatca cgctcgccca gttcatcttg agcctgctgc tggaccgcag
      421 cttccttgag ctcaccgcga ccgtcgccgg ggtcaaccat ctgccggtca tcaccgcgct
      481 cgacgtcggc ggcgaggacg ggttggcgtt gctgcgggac ctcctcgacc acgagtccga
      541 gcggggcgac gagccggtct ccaccgagtt tgtggagggc ctgggtcacg agaaggtctc
      601 cgaaggtggg gagtggacga agcgggatct gctgcacgcc aaccgggtca agctcgagct
      661 gttccgccgc ttcggggtcc tgccgggcgc gggggaccgg cacctggtcg agttcttcgc
      721 cggattcctc accgaggagt cacggtgggg cgagcgctgg ggggtcggac tcacctcgat
      781 cgaagaccgg gaacggggcc aggcggacca catccgggac ttccaccagc tggtggcgat
      841 gaccgaggtc gatgacatgc cgtcggggga gatggtggcc ccggtgatga tgtgcctgct
      901 ggaggaccag ccgggctggt tcccactcaa catcccgaac caggggcagg tcgccgatgt
      961 cgccgacgac gcggtcgtgg agagcatgtg tgtggtcgac gggtcagggg tgcggggccg
     1021 ggacgtggtg cacgtcccgc cggtgctcgc cgacacgctc cggcgcgtca gcaccgcgca
     1081 ggagctgacc gtcgacgccg cgctcaccgg tgaccgggag caggtgttcg aggcgatgat
     1141 ggtcgacccg ctcgccgggc ggctggacta cgacacgttg cgtgatctga cggacgagat
     1201 gcttgtcgcc acgaaacgct ggctcccaca gttcgcgtag ccgtggggga ggcgttcacc
     1261 gtcgacgcga tgacggtgca ggtgcacgac agcgacaccg agctggcatc cgacgcggcg
     1321 gccgaggcgg ccgaggccat cggggcggcc atcggcgccc gcggggtcgc gaacgtcatg
     1381 ttcgcgaccg ggaactccca gctcggcttc ctcgaggcgc tgaccgcccg cgacgacgtc
     1441 gactgggatc ggctggtggg gttccacatg gacgagtatc ggggagtgtc ggcggagcat
     1501 ccggcgtcgt tccggcggta cctgcgggag cggatcgtgg accgggtgca ccccggcgag
     1561 ttccagttca tcgccggtga cgccgccgac ccggcggggg agtgcgcccg ttacgcggcc
     1621 ctgctgcggg accatccgct cgacctgtgc tgcctcggca tcggggagaa cgggcacctg
     1681 gcgttcaacg acccgccggt ggccgacttc gacgaccggg tggacgtgaa ggtggtggcg
     1741 ctcgacgagg cgtgccgacg ccagcaggtc ggcgaggggc acttcgcgac ggtgcccgac
     1801 gtcccggcgg aggcgatcac ggtcacgatc ccggcgttgc tgcgggcggg tcgggtcctg
     1861 gccatcgtgc ccgacgcccg gaagcgggat ccggttcggg tggcgttgac cggtccggtc
     1921 acgacggtgt gtccggcgtc gatcctgcgc acccgcagca acgtgaccct gcacctggac
     1981 cggtcctcgg cggcggatgt cgcgccgtga ccgcgcccga gctgcacgtc gtcacgttcc
     2041 cgagcctcgc cgagttgcgc cgtcgcctcc ctcgcctcct ggccgggatc gtggtgctgt
     2101 cggccgggat cacgctgatg ctccaagccc gcctcggggt cagcgcgtac gacgtcctcc
     2161 atcagggatt gtcgaaggtc accggcctgt cgttcgggac cgtggtcgtg ctgctcggac
     2221 tggtgatcct ggtggggtgg atcccgattg ggcagcgttt cggg
//
LOCUS       DATCCH010000323         3521 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_349556, whole genome shotgun sequence.
ACCESSION   DATCCH010000323 DATCCH010000000
VERSION     DATCCH010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349556"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1852
                     /locus_tag="VIJ44_06160"
     CDS             <1..1852
                     /locus_tag="VIJ44_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165516.1"
                     /translation="FRSLDVFGHHIPLLGTWSSSSLSYHTNLNNPGPLLFDALAVPVK
                     VLGANTGLAVGVALLNLAAVAGLAVFAYRRGGALLGTLAMATVAVLGWTMGSELLFDP
                     WQPHSLLLPFLFFLVLIWSVACADLVALPFAAFVGSFVLETHLTYSVLVPVLVVFAVV
                     AVALGLRHERRRDPDRWPDRRRQLVRIGAVTAIVGVAAWTQTLIEQVTSDGKGNLTRL
                     VDAGRASHVHTVGYGLAARLAAAVIALPPWWFRPSVKDTFQPPGWHPPSLGIAVASLA
                     MTVGILAWCGWTARRHHHRTATLAVATATVALLAAVVTAAKSPITAFGAYTPHGLRFL
                     WPIAAFLFFAVLATVAIRVQASRLASARRAPERLVGAFVLVAVVFGALNLPYANLGDG
                     PNSQEWAIPAERQLQDHMSTLDGHGPLLIDDLFLGAFADPYGGAVLVELQHRGVSFVA
                     RDPGLVRQLGPARQFTGRNASATLLLRIGDATRVPPSGSHRVALGEGLSTQEQRELTS
                     LHQQLAGYLRRTGVQLSTRGQHALRRGDLPTLGGLPSGLGPGIDPEPLFTSRELNLMI
                     DGHDLVLDRAWSVRFARYSLLQGNWDRETVALFVGPVTAAKSPNGQVGRG"
     gene            1849..3366
                     /locus_tag="VIJ44_06165"
     CDS             1849..3366
                     /locus_tag="VIJ44_06165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY5165517.1"
                     /translation="MTADRDDVRERPAGFCLVVPLYNEQSRFDEFGQQLADFLNTQVV
                     GSELVFVDDGSDDATIDLVEALIAKNPSPRITLLRRPHAGKGAAVAAGLAAATAPWAA
                     FCDLDLSTPLDQLERVVHAAQRADVLAVGSRDLAASTLVRPEGPIREALGRVYNRLLQ
                     ATITPGVVDTQCGAKAASRRVWQAILPDCRETGYAWDAEAIAIARARAIPVHEIPIQW
                     HHDDRSKVNVGRDGLAMVLATPRIWRNMRRVKASAPRHEAGEVFDDANAGMLSQADTE
                     HWWFRSKAALVATAIRRVAPLDTSPGWLADVGAGSGGVTSMLGWDPGRLMVIEGHRVL
                     VNQAHHRHGLYGLQAQVDLLPLADGAADVVCLLDVLEHLADPVAALREARRVLGPDGR
                     LVINVPAHKWLWSDADEFLGHVRRYTRPALRTELAAAGFEARILTHVFSWLVPPVWVK
                     RRATKSGGPELGLDQTSPLIDRVAMALTLVERELVGRVTVPVGTSILCVAQAAAP"
BASE COUNT          477 a         1341 c         1137 g          566 t
ORIGIN      
        1 tttccgctcg ttggacgtct tcggccatca catcccactc ttggggacgt ggtcgtcatc
       61 gtcgttgtcg tatcacacga atctgaacaa ccccgggccg ctgctgttcg acgccttggc
      121 cgtcccggtc aaggtgctcg gcgccaacac cggcctggcg gtcggggtcg cgctgttgaa
      181 cctggcagcg gtcgccgggc tcgcagtgtt cgcctaccgg cgaggtgggg cgctcctcgg
      241 gacgctggcc atggcgaccg tcgcggtatt gggctggacg atgggcagcg agctgctctt
      301 cgacccgtgg cagccccaca gcttgctgct gccgttcctg ttcttcctgg tgctcatctg
      361 gtcggtggca tgcgccgacc tcgtcgcgct cccgttcgcg gcgttcgtcg gaagcttcgt
      421 gctcgagacg cacctgacct attcggtgct cgtcccggtg ctagtggtat tcgcggtcgt
      481 cgcggtcgcg ctcgggttgc gacacgaacg gcgccgcgac cccgaccgat ggccggaccg
      541 gcggcgacag ctcgtcagga tcggggcggt gacggcgatt gtcggcgtcg cggcctggac
      601 ccagacgctc atcgagcagg tcaccagcga cgggaaaggc aacctcaccc ggctcgtcga
      661 cgccggccgc gcctcccacg tccacaccgt gggctacggc ctcgcggctc gactcgcggc
      721 ggctgtgatc gcgctgccac catggtggtt ccgcccctcg gtcaaggaca cgttccaacc
      781 tccggggtgg cacccaccct cgctcgggat cgcggtggcg tcgctggcca tgacggtggg
      841 catcttggcg tggtgcgggt ggacggcccg ccgacaccac caccggaccg ccaccctcgc
      901 ggtcgcgacc gcgaccgtgg cgttgctggc agccgtcgtg acggccgcca aaagcccgat
      961 cacagcgttc ggcgcctaca ccccgcacgg cctccggttc ctgtggccga ttgcggcgtt
     1021 cctcttcttc gcagtcctcg ccaccgtcgc catccgagtc caggcgtcgc gtctcgcctc
     1081 agcccgacgg gccccggaac ggctcgtggg cgcgttcgtg ctcgtggccg tggtgttcgg
     1141 cgcgctgaac ctcccctacg ccaacctcgg tgacggcccg aactcccagg aatgggcgat
     1201 ccccgccgaa cgccagctcc aagaccacat gtccaccctc gacggccacg ggccgctgct
     1261 gatcgacgac ctctttcttg gcgcgttcgc cgacccgtac ggcggggccg tcctcgtcga
     1321 acttcagcac cgcggcgtct cgttcgtcgc tcgcgacccg ggactcgtcc gccagctcgg
     1381 ccccgcccgt cagttcaccg gccggaacgc ctccgcgacg ctcctgttgc ggatcggcga
     1441 cgcaacgcgc gtccccccat cgggttccca ccgcgtggcg ttgggtgagg ggctctctac
     1501 ccaagagcag cgcgagttga ccagcctcca ccaacagctg gccggttacc tacgacgcac
     1561 cggcgtgcag ctcagcaccc gcggacagca cgcgctccgc cgcggtgacc tccccaccct
     1621 gggtggcctg ccgtcgggac tcggtcccgg aatcgatccc gagccgctct tcacctcgcg
     1681 agagctcaac ctcatgatcg acggccacga cctcgtcctc gatcgcgcct ggagcgtccg
     1741 cttcgcgcgg tactcccttc tgcagggcaa ctgggaccgc gagaccgtcg cgctcttcgt
     1801 cggtccggtc accgcggcca agagcccgaa cggacaggtc ggacgcggat gaccgccgac
     1861 cgcgacgacg tccgagaacg gccggccggc ttctgtctcg tcgtcccgct ctacaacgag
     1921 cagtcgcgct tcgacgagtt cggacagcag ctcgccgatt tcctgaacac ccaggtggtc
     1981 ggaagcgaac tcgtcttcgt cgacgacggc agcgacgacg cgaccatcga cttggtcgag
     2041 gcgttgatcg cgaagaaccc cagcccgcgg atcacgctcc tccgtcgacc ccacgccggg
     2101 aagggcgccg cggtcgccgc cgggctcgcc gccgcgaccg cgccctgggc ggcgttctgt
     2161 gacctcgacc tgtccacccc gctggaccag ctcgaacggg tcgtgcacgc cgcccagcgg
     2221 gctgatgtgc tcgcggtcgg gtcgcgtgac ctcgccgcct cgacgctcgt ccgaccggaa
     2281 ggcccgatcc gggaggccct cggccgcgtc tacaaccggc tcctgcaagc cacgatcacc
     2341 cccggggtcg tcgacaccca gtgcggtgcg aaggccgcca gccggcgggt ctggcaggcg
     2401 atcctccccg actgccgcga gaccgggtac gcgtgggacg cggaagcgat cgccatcgct
     2461 cgcgcccggg cgatccccgt gcacgagatc cccatccagt ggcatcacga cgaccgctcg
     2521 aaggtcaacg tggggcgcga cggcttggcg atggtcctcg ccacgccgcg gatctggcgc
     2581 aacatgcgcc gggtcaaggc cagcgccccg cgccacgaag ccggcgaggt cttcgacgac
     2641 gcgaacgccg gcatgctcag ccaagccgac accgagcact ggtggttccg tagcaaagcc
     2701 gcgctcgtcg ccaccgcgat ccggcgcgtc gcgcccctcg atacgtcgcc gggatggctc
     2761 gccgacgtcg gcgccggatc cggcggtgtc acctcgatgc tcggatggga tcccggtcgc
     2821 ctcatggtca tcgagggcca ccgggtcctg gtcaaccaag cccaccaccg ccacggcctc
     2881 tacgggctcc aagcccaagt tgacctgctt cccctcgccg acggcgccgc cgatgtggtc
     2941 tgcctcctcg acgtcctcga gcacctcgcc gatcctgtcg ccgcgctgcg cgaagcccgg
     3001 agggtcctcg gacccgacgg tcgtctcgtc atcaacgtcc ctgcgcacaa atggctctgg
     3061 agcgacgccg acgagttcct cggtcatgtc cgccgataca cccgcccggc tctgcgcacc
     3121 gagctcgccg cggccggctt cgaagcccgc atcctcaccc acgtcttcag ctggctggtg
     3181 ccgccggtct gggtcaagcg gcgcgccacg aagtccggcg gccccgagct cggtctcgat
     3241 cagacctccc cgctcatcga ccgcgtcgcc atggccctga ccttggtgga acgcgagctc
     3301 gtcggtcgcg tcaccgtccc cgtcgggacg tcgatcctct gcgtcgcgca ggcggccgcg
     3361 ccctgaccga cggttagggg aggcgcttca gggcccgcag cagcccgagc cgcgcagggg
     3421 actggtgctc ggatcccgtc gtgctctcca gctgggctcg gtgcgcgagg aaccagtcgt
     3481 aggactcgac gaccatggac gcgttcgagt gcctcggctc c
//
LOCUS       DATCCH010000324         3045 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_17389, whole genome shotgun sequence.
ACCESSION   DATCCH010000324 DATCCH010000000
VERSION     DATCCH010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17389"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..728)
                     /locus_tag="VIJ44_06170"
     CDS             complement(60..728)
                     /locus_tag="VIJ44_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013425855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HEY5165518.1"
                     /translation="MTRVLGVDGCRGGWVAVALEGARFATARFAARFADLVEDEATVI
                     GVDIPLGSNGASRAADRAARRLLGRRGVSVFDAPPRDVLDAPDHATANQRCRDRYGRG
                     LSVQAWNLVPKMLDVEPHWHQAPQRIFEVHPELSFATMRDAVVEEPKQTWAGHRIRAR
                     LLAEAGIRVPDELGTAGAAGTDDVLDAAAVAWSAARIAAGEQRCVPDPAERDAANRPI
                     AIWF"
     gene            complement(725..1195)
                     /gene="greA"
                     /locus_tag="VIJ44_06175"
     CDS             complement(725..1195)
                     /gene="greA"
                     /locus_tag="VIJ44_06175"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEY5165519.1"
                     /translation="MEQVHLTRDAYERLRKELEERTTAKRTEISLWIERAREHGDIRE
                     NADYDAAKNEQGHNEGRVRHIEAILRRSVIVESSDGNLVEPGTIVEIRMDDDDQTVEY
                     LVGSIEERHESYDVLSVSSPLGQALMGAGPGQLVQYAGPRRTFKVEVVSVRPAV"
     gene            complement(1259..2113)
                     /locus_tag="VIJ44_06180"
     CDS             complement(1259..2113)
                     /locus_tag="VIJ44_06180"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY5165520.1"
                     /translation="MDATARRWVQQVVGPGSRVDESGQFRHGTSSEVTALDVVDRDGE
                     RQALVLRRFVDDAWLAREPDLAVREAAVLEALAASPVPAPALVAVDATGADAGVPTVL
                     TRRLPGTVVMAPPDLGRWLHGLVDLLAMIHATPIPPGLRLYRPWYATTRALEPPAWSH
                     DGPAWAAAISRARAPAPVGTSVFLHRDFNPTNVLWVHDRVSGVVDWVEASAGPAGADL
                     GHCRCNLAMELSLEAADALRDAFGLAEYDPYWDLISAVDFLPDLTSPRPAAQERLDAF
                     VAAAVAHL"
     gene            2295..>3045
                     /locus_tag="VIJ44_06185"
     CDS             2295..>3045
                     /locus_tag="VIJ44_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEY5165521.1"
                     /translation="METNTVHRIGIIGGDGIGPEVVAEATKVLDATDVAYTPVAFDLG
                     GHRYLRDGEVLPAADLDAIRHLDAVLLGAVGVPEVPPGVIERGLLLALRFELDLYVNV
                     RPFVASPSPHNDGVDMVVVRENTEGAYAGEGGYLRKGTPHEIATQGSVNTRFGVERCI
                     RFAFDLARRRDRKHLTLVHKTNVLTFAGDLWQRTFDDVARDYDDVHTAYHHVDAACIY
                     FVQDPGRYDVIVTDNLFGDILTDLGGAISGGI"
BASE COUNT          477 a         1118 c          997 g          453 t
ORIGIN      
        1 aaggtccgcg cccgcgatgg gtcacggcac cccaccatca cttccttccc cgaagatctc
       61 tagaaccaga tcgcgatcgg ccggttcgcg gcgtcacgct ccgccgggtc ggggacacag
      121 cgctgctcgc cggcggcgat ccgtgccgcg gaccacgcca ccgcggccgc gtcgagcacg
      181 tcgtcggtcc cggcggcacc ggcggttccc aactcgtcgg gcacccggat ccccgcctcg
      241 gcaagcaacc gggcccggat ccggtgtccc gcccacgtct gcttcggttc ctcgaccacc
      301 gcgtcgcgca tcgtcgcgaa cgacagctcg ggatggacct cgaagatgcg ctggggcgcc
      361 tggtgccaat ggggctcgac gtcgagcatc ttcgggacca ggttccacgc ctggacactc
      421 aacccgcgcc cgtaacggtc acggcagcgc tggttcgccg tcgcgtgatc cggcgcatcg
      481 agcacgtcgc gtggcggcgc gtcgaaaacc gagactccac ggcgaccgag cagccggcgc
      541 gccgcacggt cggcggcgcg tgacgcgccg ttcgacccca acgggatgtc gaccccgatg
      601 accgtcgcct cgtcctcgac gagatcggcg aagcgggccg cgaaccgagc cgtggcaaac
      661 cgggcgccct cgagcgccac agccacccag ccgccgcggc acccgtcaac gccgagcact
      721 cgggtcacac ggcgggacgg acgctgacga cctcgacctt gaaggtgcga cgcggtccgg
      781 cgtactgcac cagctgtccg gggccggcgc ccatcagggc ctgtccgagg ggcgacgaga
      841 ccgacaggac gtcataggac tcgtggcgtt cctcgatcga cccgacgagg tactccaccg
      901 tctgatcgtc gtcgtccatg cggatctcga cgatcgttcc cggctccacc aggttcccgt
      961 cggaactctc gacgatcact gagcggcgca ggatcgcctc gatgtgacgg acccggccct
     1021 cgttgtggcc ctgctcgttc ttggccgcgt catagtcggc gttctcgcgg atatcgccgt
     1081 gctcccgggc gcgttcgatc cacagcgaga tctcggtgcg cttggccgtc gtgcgctcct
     1141 cgagctcctt gcggaggcgc tcgtaggcgt cacgcgtcag gtggacttgc tccatgttcg
     1201 gagcgtaccc gccacctatg acggaaaaat catgagttga ccaagaaccg tgacagccct
     1261 acaggtgcgc cactgcggcg gcgacaaagg catcgagacg ctcctgagcc gccggtcggg
     1321 gtgacgtcag gtccgggagg aaatccaccg cgctgatgag gtcccagtac gggtcgtact
     1381 cggccaggcc gaacgcgtcg cgtagcgcgt cggcggcctc gaggctcagc tccatcgcaa
     1441 ggttgcagcg acaatggccc aggtcggccc cggccggtcc ggccgaagcc tccacccagt
     1501 ccacgacccc cgacacacgg tcgtgaaccc acaagacgtt cgtggggttg aagtcgcggt
     1561 ggaggaacac gctcgtgccc accggcgcgg gagcgcgagc acggctgatc gcggccgccc
     1621 acgcgggccc gtcgtgtgac caggccggcg gttcgagcgc ccgtgtcgtc gcgtaccacg
     1681 ggcgatagag gcgcagaccc ggcgggatcg gggtggcatg gatcatcgcc agcaggtcga
     1741 ccagaccgtg gagccagcgc ccgaggtccg gtggcgccat cacgaccgtc ccaggcaggc
     1801 ggcgagtcag caccgtcggc acccccgcgt cggctcccgt cgcgtcgacc gcgacgagcg
     1861 ccggtgccgg caccgggctg gcagccaggg cctccaggac cgcggcctcg cggaccgcga
     1921 ggtcgggttc ccgggccagc cacgcgtcgt ccacgaagcg gcgcagcacc agcgcctgcc
     1981 gttcgccgtc gcggtcgaca acgtcgagcg cggtgacctc cgagctcgtg ccgtgacgga
     2041 actggcccga ctcgtccacc ctcgatcccg gcccgacgac ctgctgcacc catcgacgtg
     2101 ccgtcgcgtc catggaaccg agtctgaccg agatcgtttg accgagccct tccaccctgc
     2161 gagactggcc tggccatgca gccgctcacc gcgaagatcg ttatcgacta gcaggcgccg
     2221 agcccggcgt ctgcgtccga ccgtccacct cggtgggcgg tttttcattg cggggaccgc
     2281 caggctgcga cgccgtggag accaacactg tgcaccggat tgggatcatc ggaggcgatg
     2341 gcatcggccc tgaggtcgtg gccgaggcga cgaaagtcct cgacgcgacc gacgtggcct
     2401 acacgccggt cgcgttcgac ctgggcggcc accggtacct gcgtgacggc gaggtcctgc
     2461 ccgctgcgga cctcgacgcc atccgccacc tcgacgccgt cctcctcggc gccgtcggcg
     2521 tccccgaggt gcccccggga gtcatcgagc ggggactcct cttggcgttg cgcttcgagc
     2581 tggacctgta tgtgaacgtc cggccgttcg tcgcctcgcc gagcccccac aacgacggcg
     2641 tcgacatggt cgtggtgcgc gagaacaccg aaggcgccta cgccggcgaa ggcggctatc
     2701 tccgcaaagg caccccccac gagatcgcca cgcagggctc ggtgaacacc cgcttcggtg
     2761 tcgagcgttg catccgcttc gcgttcgatc tagcccgccg ccgcgaccgc aagcacctga
     2821 ccctggtcca caagaccaac gtgctcacct tcgccggcga cctctggcag cggaccttcg
     2881 acgacgtcgc ccgggactac gacgacgttc acaccgcgta tcatcatgtg gacgcggcct
     2941 gcatctactt cgtacaagat cctggccgct acgacgtgat cgtgaccgac aacctcttcg
     3001 gagacatcct caccgacctc gggggtgcta tctccggtgg aatcg
//
LOCUS       DATCCH010000325         2756 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_325796, whole genome shotgun sequence.
ACCESSION   DATCCH010000325 DATCCH010000000
VERSION     DATCCH010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325796"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="VIJ44_06190"
     CDS             <1..457
                     /locus_tag="VIJ44_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165522.1"
                     /translation="GGRRLLVLVLVLATLAAAGYLYLARSSSGSATGFLGANQQSVKA
                     ASTVITAADRVQRFAELRAFDLIATEQIHTLFQQRTTLQSIAARALGRQKQIANEAVS
                     TVQQAIDSVDRYRLSVAITYRLNDADAAHQELNNAIASLQQQAQAWQHS"
     gene            492..1703
                     /locus_tag="VIJ44_06195"
     CDS             492..1703
                     /locus_tag="VIJ44_06195"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY5165523.1"
                     /translation="MDFSHAPEDEAFRDELRGWLEVNLPKFLADRGGDEDPGGGRVTG
                     GVERTQERRKDWQRRLNEGRWAAINWPAAWGGREATPVQNVIYSEEMARVRAPGIYNA
                     NGIWQIGPMIIRWGTEDQQNRWLAGILSADEHWCQGFSEPQAGSDLANLRTTAIRDGD
                     EYVVNGQKIWISTAHLAKWGLYLFRTDPEAIERGAKHEGITAFIIDMDTPGIECRPIR
                     DIVGDEMFNEVWFTDVRIPASCRLGDEGAGWQVAMGTLGHERVGTAGLAITMAADLRA
                     MISTARAVNPAALDDPEIRERIARAHTDIEFTKLLNYRALTKIIKGQKNWPEVPLAKL
                     QWSHLAQTLAELAVDLLGPNGLLARGGPDAVDGGTWTRLYSFQRYTSIGAGATEVQKN
                     IIADRALFPRK"
     gene            1776..2648
                     /locus_tag="VIJ44_06200"
     CDS             1776..2648
                     /locus_tag="VIJ44_06200"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006553019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEY5165524.1"
                     /translation="MRLGIVTPVLTRLPRAHAGWEADAGIAEVEQVVVAAERLGYEFA
                     TCSEHVAIPRPVAETRGATYWDPLSTFGYLAARTTTIRLATFVLVVGYHHPLAIAKRY
                     GTLDRISGGRLILGVGVGSLEEEFALLDIPFSDRGARADDALRAVRAAFGVTEPRYAG
                     THYQFEGFVVDPSGLQRDVPLWVGGRTARSLRRAIELGDGWAPFALDPHDVAGLLSKA
                     RQTEAWDQRSRELDVILQPVPPLDPLGAANEAREVVAAHRAAGATALAVRLRHRSVTH
                     YADQLEALADLAGT"
BASE COUNT          467 a          959 c          921 g          409 t
ORIGIN      
        1 cggcggccgg cgactcctcg tgctggtctt ggtgctcgcg accctcgccg ccgcgggcta
       61 cctctacctc gcgcggagca gcagcggcag cgccaccggc ttcctgggcg ccaaccagca
      121 gtcggtgaag gccgccagca ccgtgatcac ggccgccgat cgagtgcagc gcttcgcgga
      181 gttgcgcgcc ttcgacctta ttgccaccga gcagatccac accctgttcc agcagcggac
      241 gacgctccag agcatcgcgg cccgcgcgtt gggccgccag aagcagatcg ccaatgaagc
      301 ggtgagtacg gtccaacagg ccatagacag cgtcgatcgg taccgattgt cggtcgcgat
      361 cacctatcgc ctcaacgacg ccgacgccgc gcaccaagaa ctcaacaacg cgatcgccag
      421 cctccagcag caggcccagg cgtggcagca ctcgtgaccg tcgagcgcat catcggagca
      481 tcggactagg catggatttc tcccacgctc ccgaggatga ggcgttccgc gacgagctgc
      541 gtggatggct cgaggtgaac ctcccgaagt ttctcgccga ccggggtgga gacgaagacc
      601 ccggcggtgg ccgggtgacc ggcggggtcg agcgcaccca ggagcgccgc aaggactggc
      661 agcgacgcct gaacgaggga cggtgggccg cgatcaactg gccggccgcg tggggcggcc
      721 gggaggcgac cccggttcag aacgtgatct actccgagga gatggcccgg gtccgggccc
      781 ccggcatcta caacgcgaac ggaatttggc agatcggccc catgatcatc cggtggggaa
      841 ccgaggacca gcagaaccgt tggcttgccg gcatcttgag cgccgacgag cactggtgcc
      901 agggcttcag cgaaccgcag gccggctcgg acctcgccaa ccttcgcacg accgcgatcc
      961 gagacggcga cgagtacgtc gtgaacggcc agaagatctg gatctccacc gcgcacctcg
     1021 ccaaatgggg cctctacctg ttccgcaccg accccgaagc gatcgaacgg ggcgccaagc
     1081 acgagggcat caccgcgttc atcatcgaca tggacacccc cgggatcgag tgtcgaccca
     1141 tccgcgacat cgtcggcgac gagatgttca acgaggtgtg gttcaccgac gtccggattc
     1201 ccgcgtcgtg ccgcctcggc gacgagggcg ccggctggca ggtcgcgatg ggcaccctcg
     1261 gacacgaacg ggtcggcacc gccggcttgg ccatcacgat ggcggccgac ctccgggcga
     1321 tgatctccac ggcgcgggcc gtgaaccctg cggccctcga cgatcctgag atccgggagc
     1381 gcatcgcccg ggcccacacc gacatcgagt tcaccaagct gctcaactac cgggcgttga
     1441 ccaagatcat caagggccag aagaactggc ccgaggtgcc cctcgccaag ttgcagtgga
     1501 gccacctggc tcagactttg gccgagctcg ccgtcgacct gctcggacca aacggtctgc
     1561 tggcccgagg cggccccgac gcggtcgacg gaggtacctg gacccgcctc tacagctttc
     1621 agcggtacac gtcgatcggc gcgggtgcca ccgaggtgca gaagaacatc atcgccgatc
     1681 gggctttgtt tccccgcaag tgactccgag aaggcgatga ccggcggcaa acgtcatcga
     1741 acatcgccat ccgctgctca gccccgcgag gatgtatgcg gctcggcatc gtcacgccgg
     1801 tcctaacccg actgcctcgc gcccacgccg gttgggaagc cgacgccggg atcgccgagg
     1861 tcgagcaggt cgtcgtggcc gcagagcggc ttggctatga gttcgcgacg tgcagcgagc
     1921 acgtcgccat cccgcgaccg gtcgccgaga cccggggcgc gacctactgg gacccgctgt
     1981 ccaccttcgg ctacctggcg gcgcgcacga cgacgattcg gctcgcgacg ttcgtcctcg
     2041 tcgtcggata ccaccatccc ctcgctatcg cgaagcggta cgggaccttg gaccggatct
     2101 cgggcgggcg tctgatcctc ggggtgggtg tcggttcgct cgaagaggaa ttcgccctgc
     2161 tcgacatccc attctcggat cggggagcac gcgctgacga cgcgctacga gccgtgcgcg
     2221 ccgcgttcgg ggtcaccgaa ccccgttacg ccgggaccca ctaccaattc gagggcttcg
     2281 tcgtggatcc gagcgggtta cagcgtgacg tcccattgtg ggtcggggga cgcaccgccc
     2341 ggtcgctgcg gcgagcgatc gagctcggtg acggatgggc gccgttcgcg ctcgaccctc
     2401 acgacgtcgc cggcctgctc tcgaaggccc gccaaaccga ggcgtgggac caacggagcc
     2461 gtgagctcga cgtcatcttg cagcccgtcc cgcctctcga tccgctcggt gcggccaacg
     2521 aggcccggga ggtggtcgcg gcgcaccggg ccgcgggcgc gaccgcgctg gcggtccgtc
     2581 tccgtcatcg ctcggtcacg cattacgccg accagctcga ggcgttggcg gacctcgccg
     2641 ggacctgacc ggtcagcgga gcagcgcccg ggtgtggcgg gcaagcatca ggtcctcgtt
     2701 agtctcgagt gcccagacgg tgacgcgaga gtcgggatcg ctgatcaccg gctccg
//
LOCUS       DATCCH010000326         4162 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_119165, whole genome shotgun sequence.
ACCESSION   DATCCH010000326 DATCCH010000000
VERSION     DATCCH010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4162
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119165"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..411
                     /locus_tag="VIJ44_06205"
     CDS             154..411
                     /locus_tag="VIJ44_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009356585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEY5165525.1"
                     /translation="MPVANVLADFNDHMMNGGGGGRWIWGVLIMVVILGIVALVVWLI
                     VRASHGSAGGHSSSAREILSERFARGEIDADEYHDRLSKLR"
     gene            complement(572..1498)
                     /locus_tag="VIJ44_06210"
     CDS             complement(572..1498)
                     /locus_tag="VIJ44_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5165526.1"
                     /translation="MDSFEGRLAVVTGGGTGMGRELVIQLAAEGCSVATCDVNADTMG
                     ETSMWAGKDALADVRITTHLADVSDETQMHRFRDEVVAQHQTDHVNLLFNNAGIAGGS
                     SFLTGPRDGWDKTFAVCWGGVYNSMRAFADLLVASDEGYVINTSSVNGFWASLGPGVP
                     HTAYSAAKFAVKGFTEALMEDFRVNAPHVKVAVVMPGHVGTDIVINSRQILLMDLDEA
                     RRQVARHGVPTEGVSDDEIRDMVKTMGEMFRDNAPLTAAQAATIILDGVRAGKWRILV
                     VDDAQALDETVRADPEGAYGERGLSLGTITGP"
     gene            2144..3859
                     /locus_tag="VIJ44_06215"
     CDS             2144..3859
                     /locus_tag="VIJ44_06215"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY5165527.1"
                     /translation="MDAIEARAALETWRRSFPEDPYREEGHLRRWNRRALDPKRAAEL
                     ERAAAAFGRRVVAEVSPAAERYEHRAHLPELVGHDWLGRPTEEIRFDPDYHRAGRAVW
                     ESGVVAYVGTPGRAFEQATLLYLLSLEGEAGHACPVVCSIGLARALRRKAGPAVRDRF
                     LPALLERDYERAERGSQFLTEIQGGSDVGANSCTAAAREDGTYAISGEKWFCSIADAG
                     QFLVTARPAGARAGTAGLGCFIVPRTVGGQPNGFRVRRLKEKLGTRGLATGEIEFNGA
                     LAWPVGPLEEGFKIAAGVVLNTSRWLTAVGSTGMMRRAYLDASRFARLRCAFGRPIGE
                     FPAVRTTLARIKTHWLGALHATWLLTDLEDQIDADTASDESVALHRFLVNATKFRVSS
                     AATGVVRDAIEVLGGNGTIEDFSVLPRLYRDAIVYESWEGTHDVLVAQVLADTRELGL
                     ASVVDSRLRDLLESVSEPAAKSAVDTTLAALDGATALVLRAVDDASFGATHFRACLSE
                     VMRVVEVATLLYMADDNGQDSEELLAAADLAVRLGLDRDYRPEADPGFLDRVNAVLGS
                     EAPNP"
     gene            3966..>4162
                     /locus_tag="VIJ44_06220"
     CDS             3966..>4162
                     /locus_tag="VIJ44_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165528.1"
                     /translation="MRSNRASGEVEEGRESLDGGAAEAAPVEDLLARHRDRAAPAITA
                     EADETYRAAPDTVERSGARARS"
BASE COUNT          631 a         1412 c         1438 g          681 t
ORIGIN      
        1 gaggccggtc ccgggcccgg gccgccggga cctgctcgcc gacgtcctcg aatgcgccga
       61 ggacgagacc ggcaaccgat aggtggcgag gtttctgatg acggtgcggt cctaaggtgg
      121 acgtaggacc gcaacctcga ggaagaggcg atcatgcccg tcgcgaatgt gctcgccgac
      181 ttcaacgatc acatgatgaa cggcggcgga gggggtcggt ggatctgggg tgtcctgatc
      241 atggtcgtga ttttggggat cgtggctctc gtcgtgtggt tgatcgtgcg agcctcacat
      301 ggcagtgccg gcggtcattc atcttccgcg agggagattc tgagcgaacg ttttgcccga
      361 ggcgagatcg acgccgacga gtaccacgac cggctcagca aactccgctg aagtagagct
      421 ccggtggccg tcatcggcca gctgcttctt cccgagccgg cgcttccgcg ggacgcggac
      481 ctggtcgccg acgccatcga gtgcgccgag gacgaagcgg gcggctaggt cccggcctac
      541 gctcccaccg cgcctcggtc ctttcggaac attacgggcc ggtgatggta ccgaggctca
      601 gcccgcgctc gccgtaggca ccctcgggat cggctcgcac cgtctcgtcg agggcctggg
      661 cgtcgtcgac gacgaggatc cgccacttcc cggcgcgcac accgtcgagg atgatcgtcg
      721 ccgcctgcgc ggcggtaagc ggggcgttgt cacggaacat ctcgcccatc gtctttacca
      781 tgtcccggat ctcgtcgtcg gagacgccct cggtcgggac gccgtgacgc gccacctgcc
      841 ggcgcgcttc gtcgaggtcc atcaggagga tctgacggct gttgatcacg atgtcggtgc
      901 cgacgtggcc gggcatcacg accgcaacct tcacgtgtgg cgcgttgacc cggaagtcct
      961 ccatgagcgc ctcggtgaag cccttcaccg cgaacttcgc tgcgctgtag gcggtgtggg
     1021 gaacaccggg accgagcgag gcccagaacc cgttcacgct gctcgtgttg atgacataac
     1081 cctcatcact cgccacgagc aggtcagcga aagcgcgcat gctgttgtag acgccgcccc
     1141 agcacacggc gaacgtcttg tcccacccgt cacgtggacc agtgaggaaa ctgctcccgc
     1201 cagcgatgcc ggcgttgttg aacagcaagt tgacatgatc tgtctgatgc tgcgccacga
     1261 cctcgtcgcg gaatcggtgc atctgcgtct cgtcggacac gtcagcgaga tgggtcgtga
     1321 tccggacgtc ggccagtgcg tccttcccgg cccacatcga cgtctcgccc atcgtgtcgg
     1381 cgttgacgtc gcaggtcgcc accgaacatc cctcggccgc cagctggatc accagctccc
     1441 gacccattcc ggtgccgcct cccgtcacga cagccagccg tccttcgaac gaatccattc
     1501 gtcgacccta gccctgagag gtcgaggcct ttgctggtcg acgcgttcgg tgcgccagga
     1561 tgctcgccag tgaaggaacc cgacgaggct cgagcggttg tcgccgacgt ctcatgacgc
     1621 tcggcccggg attcgagtct cgtcgacaag atgaggcgac acgtagccgc gcccctggac
     1681 accgaacatg ccgagtgatc tgctcaacga ccgctgcacg actcggctgc ccgcgcctcg
     1741 gataggcagg ccgcgtggag gtcgctggtc ggcgacgcgg agccgggaag gagcgtcgat
     1801 ctcgtgacct gctcgttacg agcaagcaag taccgagcgc tgtgctcact ggctctgcac
     1861 cttgcgccag gccagccgcc gacgagtgac cgtgaccggg ttagctgctg tagccgccct
     1921 cgccgctatc cgcaggaacg aacgggcggt aagcggctcc tctgaagagc cttcgccctg
     1981 acacacattc agtcttgctt gttgtgttcg atgccgcgcc cgccccgagt ggtcttcctt
     2041 cccgagccgt cggcaggctt ggcccccagc acgagggcat tccgcgcccg ggccgggacc
     2101 ttcgaccctg acccagagaa cgcgttcggc gttgacttgg gacatggacg ctattgaggc
     2161 gcgcgccgcc ctcgagacgt ggaggcggtc gttccccgag gatccgtacc gggaggaagg
     2221 tcatttgcgg cggtggaacc gccgggctct cgacccgaag cgggcggcgg agctcgagcg
     2281 ggctgccgcc gcgttcggcc ggcgggtcgt cgcggaggtg agcccggccg ccgagcggta
     2341 cgagcaccgg gcccacctgc ccgaattggt cggtcatgac tggctggggc gcccgaccga
     2401 ggagattcgc ttcgatcccg actaccaccg cgccgggcgg gctgtctggg agagtggcgt
     2461 cgtcgcgtac gtggggacac cgggccgcgc gttcgagcag gcgacgctcc tctatctgct
     2521 gtcgctcgag ggcgaggcgg ggcacgcgtg cccagtcgtg tgctccatcg ggcttgcgcg
     2581 cgccctgcgg cggaaggcgg gtccggcagt gcgcgaccgg ttcctgcccg ccctcctcga
     2641 gcgcgactac gaacgcgccg agcgcggctc ccagttcctc accgagatcc agggcggtag
     2701 cgatgtcggc gccaactcgt gcaccgccgc cgcccgcgag gacggcacct atgcgatctc
     2761 gggggagaag tggttctgtt ccattgccga cgcgggccag ttcctcgtga ccgctcgccc
     2821 ggctggagcg cgcgccggca ccgccgggct gggctgcttc atcgttcccc gcacggtcgg
     2881 cgggcagccg aacggcttcc gggtgcggcg actgaaggag aagctcggga cccgggggtt
     2941 ggctaccggc gagatcgagt tcaacggcgc gctcgcatgg ccggttggtc cgctcgagga
     3001 gggcttcaag attgccgccg gcgtcgtgct gaacacgagc cgctggctca cagccgtggg
     3061 tagcaccgga atgatgcgcc gcgcgtatct ggacgcctca aggttcgcgc ggctgcgctg
     3121 cgcgttcggt agacccatcg gcgagttccc ggctgtgcgg accacgcttg cgcggatcaa
     3181 aacgcactgg ctcggtgccc tccacgccac ctggctgcta acagacctgg aggaccagat
     3241 cgacgccgac accgccagcg acgagagcgt cgcccttcac cggttcctcg tgaacgccac
     3301 caagttccgg gtgtcttcgg cggcaaccgg tgtcgtacgg gacgcgatcg aggtcctcgg
     3361 cggcaacggc accatcgagg acttcagcgt gctccctcgc ctctaccgcg acgcgatcgt
     3421 ctacgagtca tgggagggaa cgcacgacgt actcgtcgcc caggtgctcg cggacacccg
     3481 cgagctgggg ctggcgtcgg tggtggacag tcggctccgc gacctcctgg agagcgtgtc
     3541 cgagccggcc gcgaagtccg cggtcgacac gacgctggcc gcgctggacg gggcaacggc
     3601 gcttgtcttg agggccgtcg acgacgcctc gttcggcgca acgcactttc gagcctgcct
     3661 cagcgaggtc atgcgcgtcg tcgaagtcgc cacgctgctc tacatggcgg acgacaacgg
     3721 tcaagacagc gaggaactgt tggctgcagc tgatctggcc gtgcgcctgg ggctcgaccg
     3781 ggactaccgc cctgaggccg atcccggctt cctcgatcgg gtcaacgccg tgctgggctc
     3841 ggaggcgccg aacccgtagc cgcgatcagc cggagccgat gccgtcctca ccctggttgc
     3901 ggccccggga cccgaacgcg gcggcgcagc ccatggcgct ggccctcggg tgacggcgcg
     3961 ggtagttgcg ctcgaaccgc gcttcgggcg aggtcgagga gggccgggag agtctcgacg
     4021 gcggcgctgc ggaggccgcg ccggtcgagg acctgctcgc ccgacaccgt gatcgcgccg
     4081 cgcccgcgat cacggccgag gccgacgaga cctaccgggc cgcgccggat acggtcgaac
     4141 ggtccggcgc gagggcccgc tc
//
LOCUS       DATCCH010000327         3554 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_391777, whole genome shotgun sequence.
ACCESSION   DATCCH010000327 DATCCH010000000
VERSION     DATCCH010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391777"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="VIJ44_06225"
     CDS             complement(<1..377)
                     /locus_tag="VIJ44_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEY5165529.1"
                     /translation="MGYEVHTTVYEGPFDLLLHLILRQEVDLWEVSLAAIVDQYLAEV
                     DRMETLDLEIATEFLLIAATLIELKTRRLLPTPEDLELDEELLRFEERDLLLARLLEC
                     KTFKDAAAAITSLMRVADRSVARR"
     gene            435..1424
                     /gene="trpS"
                     /locus_tag="VIJ44_06230"
     CDS             435..1424
                     /gene="trpS"
                     /locus_tag="VIJ44_06230"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019134947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEY5165530.1"
                     /translation="MARVFSGIQPTGDKHLGNLLGAIRHWVATQDADDALYCVVDLHA
                     LTVPYDPKDLAARTRSTAGLLLAAGLDPQRCTLFVQSQVPAHTELTWILNCVATFGEL
                     RRMTQFKEKSAGRESVSVGLFDYPVLMAADILAYDTERVPVGDDQRQHLELSRDLALR
                     FNHRFGPTLVVPEPAIPPVAARIMDLQTPTAKMSTTAESPLGTIGLLDPPEVLSKRIR
                     AAVTDSGSEVRFDPDAKPGVSNLLQILAATTDRTIPDVEAEFAGAGYGTLKQTVADAV
                     IEFLTPVQERYRALAADPTHVDAALATGAAKADALSAAVLRRVRDAVGLLSRP"
     gene            complement(1431..1964)
                     /locus_tag="VIJ44_06235"
     CDS             complement(1431..1964)
                     /locus_tag="VIJ44_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165531.1"
                     /translation="MSRAAHLVRRFLGALRPGCPSAPDLAWVDTLLEPGELDLWQRMP
                     GHDRRHSVGVARRVEGALAPTDQAGDRRWLAAALLHDVGKLDAELGVCGRVAATLAGA
                     AAGHQWAEVWSSRRGVTRRVGLYLRHPELGATRIRVAGGHEEAAVWADAHHTQQAWAE
                     TGFPPVVVDALVAADDD"
     gene            complement(1961..2611)
                     /locus_tag="VIJ44_06240"
     CDS             complement(1961..2611)
                     /locus_tag="VIJ44_06240"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEY5165532.1"
                     /translation="MPPLREAVIFFGCLVVAVILHEVSHGVVAYWLGDDTAKRAGRLT
                     LNPVPHIDPFGSIILPLMGALAGLPVFGYAKPVPVNPARLRHPRRDLVFVSLAGPSTN
                     LLLMAIAALVARAIYSGGGVLPGAIDQSTSDLVLEIVFIFALVNLLLGLFNLLPIPPL
                     DGSALLERVLPESSLETWYRIRPFGIIVLLGLIFFTGVLGTVIQPFFNALRNFVVA"
     gene            complement(2615..2953)
                     /locus_tag="VIJ44_06245"
     CDS             complement(2615..2953)
                     /locus_tag="VIJ44_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEY5165533.1"
                     /translation="MADASHTVVRAQLQEERERLQDQLRELGHGPDGNLTFDGGFADS
                     SQVTAERGEMEALAVSLADRLGEIKEALKKLETGGYGQCEGCGQPIAEARLEAMPDAR
                     LCIVCASKSR"
     gene            complement(2958..>3554)
                     /locus_tag="VIJ44_06250"
     CDS             complement(2958..>3554)
                     /locus_tag="VIJ44_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014360197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEY5165534.1"
                     /translation="ETDVEALLAAASAENPTGLRDRAILEVLYAGGLRITELVGLDRR
                     DLDDDTGLLRVYGKGGKERVVPLGGPARVAVRAYLARGRTVLARPGRSRTRAGDAIFL
                     NARGGRLTRQGVWGLVRAAGRRAGLGDQVYPHVLRHSCATHMVDRGADIRVVQELLGH
                     ASVSTTQVYTKVSAERLRAVYDAAHPRARGESRRGAVP"
BASE COUNT          585 a         1336 c         1137 g          496 t
ORIGIN      
        1 cggcgcgcca cgctgcggtc cgcgacgcgc atcaaggagg tgatcgcggc ggcggcgtcc
       61 ttgaacgtct tgcattcgag gagccgggcg aggagcaggt cgcgctcctc gaaccggagc
      121 agctcctcgt cgagctcgag atcctcgggg gtcgggagca gccggcgggt cttcagctcg
      181 atcagcgtcg cggcgatgag caagaactcg gtggcgatct cgaggtcgag ggtctccatc
      241 cggtcgacct cggcgaggta ctggtcgacg atcgcggcga gggacacctc ccacaggtcg
      301 acctcctggc ggaggatgag atgcaagagg aggtcgaacg gaccctcgta gacggtggtg
      361 tgcacctcgt agcccatctc ggacctcaag atacccgcac cgctcccgag cacctcgcca
      421 ctaccgttcc cgggatggca cgggtcttct cgggtattca gcccaccgga gacaagcacc
      481 tcggcaacct gctgggcgcg attcgccatt gggtggcgac ccaagacgct gacgacgccc
      541 tctactgcgt cgtggatctt cacgccctca ccgtcccgta tgacccgaag gacctcgcgg
      601 cgcgcacccg cagcaccgcg gggttgctcc tcgccgcggg gctcgacccg cagcgctgca
      661 ccctgttcgt gcagagccag gttcccgccc ataccgagct cacctggatc ctgaactgcg
      721 tcgccacctt cggtgagctg cgccgcatga cccagttcaa ggagaagtcc gcgggccgag
      781 agtcggtgag cgttggactt ttcgactacc cggtgctgat ggccgccgac atcctcgcct
      841 acgacaccga gcgggtcccg gtcggagacg accaacgtca gcatctcgag ctgtcgcgcg
      901 acctggcgtt gcggttcaac catcgcttcg gtcccaccct cgtggtcccg gagccagcca
      961 tccctcccgt cgcggcgcgg atcatggacc ttcagacccc gaccgcgaag atgtcgacga
     1021 ccgcagaatc accgctgggc accatcggcc tcctcgatcc gcccgaggtg ctgagcaaac
     1081 ggatccgcgc cgcggtgacc gactccggct ccgaagtgcg cttcgacccc gacgccaagc
     1141 ctggggtgtc gaacctgctc cagatcctcg ccgccaccac cgaccggacc atccccgacg
     1201 tcgaagccga gttcgccggc gccggttacg ggacgttgaa gcagaccgtc gccgacgccg
     1261 tcatcgagtt cctcactccc gtccaggagc gataccgggc cctcgccgcc gacccgacac
     1321 acgtcgacgc cgcgctcgcc accggcgcgg cgaaggccga cgccctctcg gctgcggtgc
     1381 tgcgccgggt ccgcgacgcg gtcggtctgc tgtcccgccc ctgagtgacg tcagtcgtcg
     1441 tcggcggcga cgagcgcgtc gacgacgacc ggcgggaagc cggtctcggc ccaggcctgc
     1501 tgggtgtggt gcgcgtcggc ccacaccgcg gcttcctcgt gcccgcccgc gacccggatt
     1561 cgcgtcgcac cgagctcggg atgacgcaag tagaggccga ctcgtcgggt gacgccgcgc
     1621 cgcgacgacc acacctcggc ccactgatgt cccgcggcag cacccgccag ggtcgccgcg
     1681 acccgcccgc acaccccaag ctcggcgtcg agcttgccca cgtcgtggag cagcgccgcg
     1741 gccaaccatc gccggtcgcc ggcctggtcg gtcggcgcga gggcgccctc gacccgacgc
     1801 gccacaccaa ccgagtgtcg tcgatcatgt cccggcatcc gctgccacag gtcgagctcg
     1861 cccggctcga gcagcgtgtc gacccacgcc aaatcgggtg ccgacgggca tccgggccgc
     1921 aacgcgccga ggaaccggcg gaccaggtgc gcggcgcggc tcatgcgacg acgaagttgc
     1981 gcaacgcgtt gaagaacggc tggatcacgg tcccgagcac cccggtgaag aagatcaggc
     2041 cgagcaggac gatgatcccg aacggacgga tccgatacca ggtctccagc gacgattcgg
     2101 gcaggacccg ctccaacagg gccgaaccgt cgagcggcgg gatcggcaac aggttgaaca
     2161 gcccgaggag caagttgacc aacgcgaaga tgaacacgat ctcgagcacc agatcggacg
     2221 ttgactggtc gatcgcgccg ggcagcaccc caccaccgga gtagatggcc ctcgccacga
     2281 gcgccgcgat cgccatcagc aacaagttgg tggacggccc cgcgaggctc acgaagacca
     2341 ggtcgcggcg cgggtgacgc aatcgggccg ggttcaccgg cactggcttg gcgtacccga
     2401 acaccggcag gcccgccagc gcccccatta acggcaagat gatcgacccg aacgggtcga
     2461 tgtgcgggac cgggttgagg gtgagacgac cggcccgctt ggcggtgtcg tcacccagcc
     2521 agtaggcgac gacgccgtgg ctcacctcgt ggagaatcac cgcgacgacg agacagccga
     2581 agaagatgac cgcttctcga agcggaggca ccgctcagcg cgacttcgag gcgcacacga
     2641 tgcacagccg cgcgtcgggc atcgcctcca ggcgagcctc ggcgatcggc tgcccgcacc
     2701 cctcacactg cccgtagcca ccggtctcga gcttcttcaa ggcttctttg atctcaccca
     2761 accgatccgc gaggctgacg gccaacgcct ccatctcacc ccgctccgcc gtcacctgac
     2821 tggaatcggc gaacccgccg tcgaaagtca ggttcccgtc cggcccgtgt ccgagctcgc
     2881 ggagctgatc ctggaggcgt tcgcgctcct cctggagctg ggcgcgcacc accgtgtgag
     2941 acgcgtcagc catgccgtca tggtaccgcc cccctcctcg attctcccct ggcccgggga
     3001 tgcgccgcgt catagaccgc ccggagccgc tccgccgaca ctttcgtgta cacctgcgtc
     3061 gtcgacacgc tggcatggcc cagcagctcc tggaccactc tgatgtcggc accccgatcc
     3121 accatgtgcg tcgcacacga gtggcgcagc acatgggggt acacctggtc gccgagccca
     3181 gcccgccggc ccgcggcgcg caccagcccc cacacccctt ggcgggtgag ccgtcccccg
     3241 cgggcgttca agaagatcgc gtcgcccgcc cgcgtcctgg accgtcccgg ccgcgccagc
     3301 acggtccgac cccgcgccag gtaggcgcgc accgcgaccc gggcgggacc accgagaggg
     3361 accacccgct ccttgccgcc cttcccgtag acccgcagca acccggtgtc gtcatcgagg
     3421 tcacgccggt cgagaccgac gagctccgtg atccgcagcc cgccggcgta gagcacctcg
     3481 aggatcgccc ggtcccgcag ccccgtcggg ttctcggccg acgccgccgc cagcagcgcc
     3541 tcgacgtccg tttc
//
LOCUS       DATCCH010000328         2363 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_493229, whole genome shotgun sequence.
ACCESSION   DATCCH010000328 DATCCH010000000
VERSION     DATCCH010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493229"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /locus_tag="VIJ44_06255"
     CDS             complement(<1..930)
                     /locus_tag="VIJ44_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020100449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY5165535.1"
                     /translation="MEPLRIGTLGAARITPTALIRPARRVPEVEVVAVAARDRERAER
                     FARKHKIPTVHGSYDDLIADPTVDAIYNPLPNGLHGRWTLAALKAGKPVLCEKPLTAN
                     ADEAEQVVSAARDTGLVVMEAFHYRYHPLVDRVLEIIRGELGPLRRVETRMCIPLPSR
                     KDIRYQLDLAGGAVMDVGCYAIHQARTLAGAEPEVVSARARCISPGVDRWLKADLQFS
                     DDCTGSFECALWSRRLLSLGARVEGEHGTLTVFNLTGPQFYHRVVVRTRDLATGAETK
                     RRERVKGEHTYWYQLNAFVAAVRDGAPYPTTPAD"
     gene            1000..>2363
                     /locus_tag="VIJ44_06260"
     CDS             1000..>2363
                     /locus_tag="VIJ44_06260"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY5165536.1"
                     /translation="MIGSPRALLEGIRVVDCSTEIAGPYCTKLLADAGADVVKVEPPD
                     GDPLRSWGSGALFEFLHTSKRSIVAAPGDATVGELGATADVLVDTGGFGLNIPALREE
                     HPALVAASISPFGLDGPWADWAATEFTLQGWCGSTGGRGLPEGPPLAAGGRIGEWMAG
                     TYAAVAVLAALRSAARSGHGEHIDVAILDCMALTMNTYTSVFAEFLGWPPLRRPTRMI
                     EIPSIEPTADGYVAFTTNSAQQFSDFLVLIGRSDKLEDRELAMHLGRFKRRLEMIEMT
                     SDYTTRHTTAELLEEAALLRIPSGPVGNGATVPGFDHFMERKVFVESPTGRFVQPRIP
                     YRISGVEPRPFLPAPQLGEHDGTIDWSPSGDGRGSDRDANASPSLPLDGVRIIDCTAW
                     WAGPAATHALACLGADVIKIESVSRPDLMRYTSTVRPPEPQWYEWGPLFHGANNSKRA
                     VTLDL"
BASE COUNT          349 a          828 c          817 g          369 t
ORIGIN      
        1 gtcggccggc gtcgtcgggt acggcgcgcc gtcgcgcacc gcggcgacga aggcgttgag
       61 ctggtaccag taggtgtgtt cacccttgac gcgctcgcgt cgcttggtct cggcacccgt
      121 ggccaggtca cgggtccgga cgacgacccg gtggtagaac tgggggccgg tcaggttgaa
      181 cacggtgagg gtcccatgct cgccctcgac ccgggcaccc aggctgagta gccgccgcga
      241 ccagagcgcg cattcgaacg acccggtgca gtcgtctgag aactgcaggt cggctttgag
      301 ccagcgatcg acgccggggg agatgcacct tgcccgggcc gacaccacct cgggctccgc
      361 gccggcgagg gtccgggcct ggtggatcgc gtaacagccg acgtccatca ccgcgccacc
      421 ggcgaggtcg agctggtagc ggatgtcctt gcgagacggc agcgggatgc acatgcgggt
      481 ctcgacccga cgcaacggac cgagctcgcc tcggatgatc tccagcaccc gatcgacgag
      541 ggggtgatac cggtagtgga acgcctccat gacgaccaga ccggtgtcgc gcgccgccga
      601 cacgacctgt tcggcctcat cagcgttcgc ggtgagcggc ttctcgcaca gcaccggctt
      661 gccggctttg agcgcggcga gggtccagcg cccgtgcagc ccatttggga gcgggttgta
      721 gatggcgtcc accgtggggt cggcgatcag gtcgtcgtag gagccgtgca ccgtcgggat
      781 cttgtgcttg cgcgcgaacc gttcggcccg ctcgcgatcg cgggccgcga ccgcgaccac
      841 ctcgacttcg gggacgcgcc gggcgggtcg tatcagcgcc gtgggtgtga tccgggcggc
      901 tcccagcgtc ccgatgcgca acggttccat cggcccgagc ctatggccgg cgccgtggcg
      961 gggcgaggaa gccgtgcgcg agcatgcagg cccacacgct tgatcgggag cccgcgcgcg
     1021 ttgctcgaag gtatccgggt cgtcgactgc tcgaccgaga tcgccggccc ttactgcacg
     1081 aagttgctcg ccgacgccgg cgccgacgtc gtcaaggtcg aaccgccgga cggtgaccct
     1141 ctgcgctcgt ggggctcggg cgccctcttc gagttcctcc acacctccaa gcgatcgatc
     1201 gttgccgcgc ccggtgacgc caccgtcggt gagctgggcg cgaccgccga cgtcctcgtc
     1261 gacaccggcg ggttcgggct caacatcccc gcgctccgcg aggagcaccc cgcgctcgtc
     1321 gcggcgtcga tcagcccgtt cgggctcgac ggcccctggg ctgactgggc cgcgaccgag
     1381 ttcaccctcc agggctggtg cggatcgacc ggaggccggg gcctccccga ggggccgccg
     1441 ttagcggctg gcgggcggat cggcgagtgg atggccggca cctacgccgc cgtcgcggtc
     1501 ctcgccgcgc tgcgatcggc ggcccgatct ggacatggcg agcacatcga cgtcgcgatc
     1561 ctcgactgca tggccctgac catgaacacc tacacgtcgg tgttcgccga gttcctcggc
     1621 tggccgccgc tgcgtcggcc gacccggatg attgagatcc cgtccatcga gccgacggcc
     1681 gacgggtatg tggcgttcac gaccaacagt gcacagcagt tttcggactt cctcgtcctc
     1741 atcggccggt ccgacaagct cgaggatcgt gagctcgcga tgcacctcgg tcggttcaag
     1801 cggcgcctcg agatgatcga gatgacgtcc gactacacga cccgtcacac cacggcggag
     1861 ctcctggagg aggcggcgct gctgcggatc ccgagcggcc cggtcggcaa cggcgcgacc
     1921 gtccccggct tcgaccattt catggaacgg aaggtcttcg ttgagagccc gacgggtcgg
     1981 ttcgtgcagc cccgaatccc gtaccgaatc tcgggcgtcg agccccgtcc cttcctaccc
     2041 gcgccgcagc tcggcgagca cgacgggacc atcgactggt caccgtcagg tgacggtcgc
     2101 ggatccgatc gggacgcgaa cgcgagcccg tcgctgccat tggacggtgt gcgcatcatc
     2161 gactgcaccg cctggtgggc cggcccagcg gccacgcacg cgctggcatg cctcggcgct
     2221 gacgtcatca agatcgaatc ggtctcccga cccgacctga tgcgctacac cagcaccgtt
     2281 cgaccgcccg agccgcagtg gtatgagtgg ggaccactgt tccacggcgc gaacaacagc
     2341 aagcgggccg tcacgctcga cct
//
LOCUS       DATCCH010000329         2630 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_225232, whole genome shotgun sequence.
ACCESSION   DATCCH010000329 DATCCH010000000
VERSION     DATCCH010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225232"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1430
                     /locus_tag="VIJ44_06265"
     CDS             <1..1430
                     /locus_tag="VIJ44_06265"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017594011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HEY5165537.1"
                     /translation="EFGARSVIDRFAQVEPTVLLAIDGYRYGGKDFDRGEIVDRIASE
                     IPGLSAVVRFGYADGSGWEDGFLGPEGESLEFASLPFDHPLWVLYSSGTTGLPKPIVH
                     GQGGILLEQLKAMNVHLDAQAGDRVFWFSTTGWMMWNFLVGVLLTDAAIVLFDGNPGY
                     PSLDVLWDLAAESEMTTFGTSAAFIASCMKGDVDPAGGGRDLSALRAVGSTGSPLSPD
                     GFEWVYEKVGADTWLFSTSGGTDMCTAFVGGVPVLPVYLGELQARALGADVQSWDPDG
                     NALIGEVGELVITSPMPSMPIFFWGDDDGSRLRESYFEMYPGVWRHGDWIEITSRGTA
                     IISGRSDATINRGGVRMGTSEIYRAVLDVEPVVDALVVDLPREGTDGWMPLFVVLRDG
                     AELDDELVAEIRRRVREDCSPRHVPNEIRQIAEVPRTLSGKILEVPVKRILTGTPADQ
                     AASRESLANPAALDYFVELAADDRG"
     gene            1771..>2630
                     /locus_tag="VIJ44_06270"
     CDS             1771..>2630
                     /locus_tag="VIJ44_06270"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY5165538.1"
                     /translation="MTTTVVQSSTRAVPTAFSAGAGMSINQIYKSDSPGVVDILVTTS
                     GGQGQSLLGGGSQQSQAEGAGVVYDTKGDILTDEHVVSGATSVKVNLSNGKSYPAKVL
                     GTDASTDVGVIHIDAPATELHPIALADSDAAQVGDPVVAIGSPFSLPETTTSGIVSQT
                     GRSIQAPNGYTIPNAIQTDAAINPGNSGGPLLDASGHVLGLNDQIETNNSTAGGEGSS
                     SGVGFAIPSNAVKRIADGIISGNPIKHPYVGIELRNSSSGGAQVGTVQQNSPATAAGL
                     QAGDVVTAVNG"
BASE COUNT          424 a          876 c          885 g          445 t
ORIGIN      
        1 cggagttcgg cgcgcgcagc gtgatcgacc gcttcgcgca ggttgagccg acggtgctgc
       61 tggcgatcga cggctatcgc tacgggggta aggattttga tcggggcgag atcgtggacc
      121 ggatcgcgtc ggagattccg gggctctctg cggtcgttcg cttcggttac gccgatgggt
      181 cggggtggga ggacgggttc ctcggtcccg agggcgagtc gctggagttc gcgtcgttgc
      241 cgtttgatca tccgttgtgg gttctctaca gctcggggac gaccggattg cccaagccga
      301 tcgtccatgg tcagggcggg atactgctcg agcagctcaa ggcgatgaac gtccatctcg
      361 acgcccaggc gggcgaccgc gtgttctggt tctcgacgac cggctggatg atgtggaact
      421 tcctcgtcgg ggtgctgttg accgacgccg cgatcgtgct gttcgacggc aacccggggt
      481 atccatcact cgatgtgctg tgggatctcg ccgccgagtc cgagatgacg acattcggga
      541 cgagcgcggc gttcatcgcg tcctgcatga agggcgacgt ggatccggcg ggcggcggtc
      601 gtgacctgag cgcgttgcgg gcggtcggct cgaccggctc gccgttgtct cccgatggct
      661 ttgagtgggt gtatgagaag gtcggcgccg atacatggct gttctcgacg tcgggcggga
      721 cggacatgtg cacggcgttc gtgggcgggg tgccggtgct cccggtgtat ctgggcgagt
      781 tgcaggcccg cgcgctcggt gccgacgtgc agtcctggga cccggacggc aacgcgctca
      841 tcggcgaggt cggtgagctg gtgatcacct cgccgatgcc gtcgatgccg atcttcttct
      901 ggggcgacga cgacggctcg cggttgcggg agtcctattt cgagatgtat ccgggcgtgt
      961 ggcgccacgg cgactggatc gagatcacct cccgggggac ggcgatcatc tccggacgct
     1021 ctgacgcgac gatcaaccgc ggcggcgtgc ggatggggac cagcgagatc taccgggcgg
     1081 tgctcgacgt cgagcccgtc gtcgacgcgc tcgtcgtcga cctgccgcgc gaaggcaccg
     1141 acgggtggat gccgctgttc gtcgtcctgc gcgacggcgc tgagttggac gacgaactcg
     1201 tcgccgagat ccgccggcgg gtgcgcgagg actgctcgcc gcgccacgtc cccaacgaga
     1261 tccgccagat cgccgaggtc ccccggaccc tcagcggcaa gatcctcgag gtcccggtca
     1321 aacggatcct caccggaacc ccggccgatc aggccgcctc ccgcgagtcc ctggccaacc
     1381 ccgcagccct ggactacttc gtcgagcttg ccgccgacga ccgcggttga accccggacg
     1441 gccacgcctc tttggccggg ggcgatcttg gccgatatac agtcaaaatc gcccacagcc
     1501 ctcaggggcc tgacgttcgc gaggagtcct ggcgtgccgg ggacccgtcc gtcctaccct
     1561 ggtcttacgg acttcttgtc gaactctgaa agctttcaca ccggaggttg gtgccattga
     1621 tccaggaaca ccaacctctg acctcaattt aggagagaca ccacaaccat gcgcaaactc
     1681 agccgatgga ttcccatcat cggcggcgcc gtcgcggggg gagtcatcgc gctggttatc
     1741 gccagcggcc acggaagctc gactcgcaac gtgacgacga ccgtcgtgca atcgagcacg
     1801 cgtgctgttc cgaccgcctt cagcgccggc gccgggatga gcatcaacca gatctacaag
     1861 agcgacagcc ccggcgtggt cgacattctc gtgacgacca gcggcgggca gggccagtcg
     1921 ctgttgggcg gcggctctca gcagagccag gccgagggcg cgggcgtcgt ctatgacacc
     1981 aagggcgaca tcctcaccga cgagcacgtc gtctccggtg ccacctcggt caaggtgaat
     2041 ctctcgaacg gcaagtccta tccggccaag gtgctgggca ccgacgcctc gaccgacgtc
     2101 ggcgtgatcc acatcgacgc gccggccacc gagcttcatc cgatcgcgct cgccgactcc
     2161 gacgccgctc aggttggtga ccccgtggtg gccatcggca gcccgttcag tctgccggag
     2221 acgaccacct cgggcatcgt cagtcagacc ggccgcagca tccaggcccc gaacggctac
     2281 acgatcccca acgcgatcca gaccgacgca gcgatcaacc ccggcaactc gggcggcccg
     2341 ctgctcgacg cctccgggca cgtcctgggc ctcaacgacc agatcgagac caacaactcg
     2401 acggccggcg gcgagggctc gagttccggc gtgggctttg ccattccgtc caacgcggtc
     2461 aagcggatcg ccgacggaat catctccggc aacccgatca agcaccccta cgtgggtatc
     2521 gagctgcgca acagctcctc cggcggcgcc caggtcggca ccgtccagca gaacagcccg
     2581 gccaccgccg caggactgca ggccggtgac gtcgtcaccg ccgtcaacgg
//
LOCUS       DATCCH010000330         2030 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_142165, whole genome shotgun sequence.
ACCESSION   DATCCH010000330 DATCCH010000000
VERSION     DATCCH010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142165"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2030
                     /locus_tag="VIJ44_06275"
     CDS             <1..>2030
                     /locus_tag="VIJ44_06275"
                     /inference="COORDINATES: protein motif:HMM:NF036578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HEY5165539.1"
                     /translation="EALRVDGLTAPIGLGANDIQFGWQVNDRRRGAIQSAYRIVVSRP
                     AVTGSPGAETPVWDSGRIAGSDQFAVPYLGPALAPDAAYQWTVQTWGLSDGPGPLAAP
                     ARFETGLTDQDWHAQWIQRPSSEPDQHTYARKEFTLSESPIVRARVYVSADQQYELSI
                     NGVRIGKGQAYSYPDSQYYETLDATRVLRAGHPNALGLLYSWQGATKGHPAGSPGVIA
                     QLSVLHQDGSSELITTDSSWRVLKGNWLPGTQRDLEGDQVDFTENIDGAHQPVGWDLP
                     GFDDRNWAAATALGPAGVAPWTHLVAVRTRVVEQPVRAVSLTRLSNGSVVADFGKVYA
                     AVPTVAFHHGTTGRRVAMHAGYLLDTHPSSGPPGQVSTVHGTQHTDLSYSYTQRGGRE
                     MFHPFDYLGFRYLQIDKPGETLAPSDVVALTRHTVMPDEPPATFSSPDPTVNAVFDLG
                     RHSALFTAQEQYVDTPTREKGPWLWDGFNESQTAMAAFGDQNLTRKSLLEFAQSQARY
                     WPQGRINKIYPTGLGALDINEFSEIYPEWVWQYWLATGDRSLLEAVYPTIANLSDYVQ
                     SAVAPSTGLVTSLPATNVYYPFPVVTRLNVLGVDVFRRAADVATALGRPTAEISLQQH
                     RQSELTTAINARLTRPDGIYVDGLLASGKQTKEATQESNTAAVAYNVVPVNR"
BASE COUNT          363 a          772 c          610 g          285 t
ORIGIN      
        1 ggaggcgctc cgagtcgacg ggttgaccgc tccgatcggt ctcggtgcga acgacatcca
       61 gttcgggtgg caggtcaacg atcggcgccg aggtgcgatc cagagcgcct atcggatcgt
      121 cgtgagccgg cctgccgtca ccggaagccc gggcgccgag acgccggttt gggacagcgg
      181 ccggatcgcg gggagcgacc agttcgcggt gccgtacctc ggccccgcgc tcgcccccga
      241 cgcggcctac cagtggacgg tgcagacctg gggcctgtcg gatggacccg gcccgctcgc
      301 ggcgccggcg cggttcgaga ccggcctcac cgaccaggac tggcacgccc agtggatcca
      361 gcgtccgagc agcgaacccg accagcacac gtacgcgcgg aaggagttca ccctcagcga
      421 atccccgatc gtgcgcgccc gcgtctacgt ctccgcggat cagcagtacg agttgtcgat
      481 caacggggtc cggatcggca aaggccaggc gtacagctac cccgactcgc agtactacga
      541 gacgctcgac gccacccgcg tccttcgggc cgggcacccg aatgcgctgg gcctgctgta
      601 cagctggcaa ggcgccacga agggacatcc ggccggcagt ccgggggtca tcgcgcagct
      661 ctccgtgctc caccaggacg ggagcagcga gctcatcacg accgacagct cctggcgcgt
      721 cctcaagggc aactggctcc cgggaaccca acgcgacctc gagggcgacc aggtcgactt
      781 caccgagaac atcgacggag cccatcaacc cgtcgggtgg gacctccctg gcttcgatga
      841 ccggaactgg gccgccgcca ccgccctcgg ccccgccggt gtggcccctt ggacccacct
      901 cgtcgcggtg cggacccgcg tcgtcgaaca gcccgtccgc gcggtctcac tgacccgact
      961 ctcgaacggc tccgtcgtcg ccgacttcgg gaaggtgtac gccgcggtcc cgacggtcgc
     1021 gttccaccac ggcaccaccg gccgacgagt tgccatgcac gccggctacc tcctcgacac
     1081 gcacccgtcg tcgggtcccc cgggccaggt ctcgaccgtc cacggaaccc agcacaccga
     1141 cctgagctac tcgtacaccc agcgcggcgg ccgagagatg ttccacccct tcgactacct
     1201 cgggttccgg tacctccaga tcgacaagcc cggcgagacg ctggcaccgt cggacgtcgt
     1261 cgcgctcacc cgacacaccg tcatgcccga cgagcctccg gccacgttct cgtctcccga
     1321 cccgacggtg aacgcagtgt tcgaccttgg ccgccattcg gcgctcttca ccgcccaaga
     1381 gcagtacgtc gacacgccga cgcgcgagaa aggcccgtgg ctctgggacg ggttcaacga
     1441 atcgcagacc gcgatggccg ccttcggcga ccagaacctc acccgcaagt ccctgctcga
     1501 gttcgcccaa tcccaggccc gctactggcc ccaaggtcgg atcaacaaga tctaccccac
     1561 cgggttaggt gccttagaca tcaacgagtt cagcgagatc taccccgaat gggtgtggca
     1621 gtactggctc gcaaccggag atcgcagcct cctcgaagcc gtctatccca caatcgccaa
     1681 cctctcggac tacgtgcaga gcgcagtcgc accgtcgacc gggctcgtca ccagtctccc
     1741 ggccaccaac gtctactacc cgttcccggt cgtgacgaga ctcaacgtgc tcggagtgga
     1801 cgtcttccgg cgcgccgcgg acgtcgcgac cgccctcggc cgacccaccg cagaaatctc
     1861 actgcagcaa caccgccaat ccgagctgac gaccgcgatc aacgctcggc tcacacgacc
     1921 cgacggcatc tacgtcgatg ggctcctcgc cagcggcaaa cagaccaagg aagccaccca
     1981 ggagtccaac accgccgcgg tcgcgtacaa cgtcgtaccg gtcaaccgcg
//
LOCUS       DATCCH010000331         3499 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_225286, whole genome shotgun sequence.
ACCESSION   DATCCH010000331 DATCCH010000000
VERSION     DATCCH010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225286"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="VIJ44_06280"
     CDS             <1..698
                     /locus_tag="VIJ44_06280"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEY5165540.1"
                     /translation="EQASELRAHLTQLGGRVVELPTIRIEPLPVALPPLDDVDWLVLT
                     SANGVSALFERGITSAGRDARALAGVRIAAIGPGTAAALARYGVRADLVPDRYVAEAL
                     LAAFPPPTGTGGRVLLARAEQARDLLPDGLRKLGYDVDVLPLYRTRAAEPDPAILDQV
                     RRGQVDAITFTSSSTVRNFVELVGTLDPQPPVVSIGPITSATARELGLRVDAEAVTHT
                     IDGLLTVLLDTLA"
     gene            752..1729
                     /gene="hemB"
                     /locus_tag="VIJ44_06285"
     CDS             752..1729
                     /gene="hemB"
                     /locus_tag="VIJ44_06285"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020215570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEY5165541.1"
                     /translation="MPFPEQRPRRLRRTSAWRRLVAEARVGIDDLVAPLFVKEGLTDP
                     EPVASMPGVVQHTVESLRKEVRALAGLGIPAVVLFGVPATKDARGSGADDPDGVLAVA
                     LRAMRDEVGDDVVLVADTCLDEYTDHGHCGVLGPDGTVDNDATLERYGSTAVTQAGAG
                     ADVVAPSGMMDGQVAAIRAALDTAGQTDTAILAYAAKYASALYGPFRDAAESTPSFGD
                     RRAYQMDPANGREAETEVALDIAEGADLVMVKPALAYLDVVSRVRAATAVPLGAYHVS
                     GEYAMVEAAAANGWFDGDAVMLEHLTAIKRAGADFILTYAARRVAERLQ"
     gene            1726..3006
                     /gene="hemL"
                     /locus_tag="VIJ44_06290"
     CDS             1726..3006
                     /gene="hemL"
                     /locus_tag="VIJ44_06290"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011602224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEY5165542.1"
                     /translation="MSDELWTRALARIPGGVNSPVRSFASVGRDPFFVARGDGAYLIA
                     TDGTRYVDYVQSWGASILGHAHPAVVEAVQRAAAAGTSYGAPTAAEVELAEAVADRVA
                     SVEKLRLVSSGTEAAMTAVRLARGATGRSKIVKFAGGYHGHVDSLLVAAGSGVATLGI
                     PGSAGITDGAVADTLVVAYNDEAALDAVFDEHGDDLAAVLVEPVAANMGLVPPAPGFL
                     AGLRHRCDLADALLVFDEVITGFRVGPAGAQGRTGITPDLTMFGKVLGGGLPLAAIGG
                     PSALLDELAPVGPVYQAGTLSGNPLATAAGLAVLARLDAALYDDLERRAGRLTDGLAG
                     AIRDAGFAVQVPRAWTLGGLFFADAPVTDYEAARAADAKRYAQFFQAMLARGVFLAPS
                     PFETLFPSVAHDDAVIDRTVAAAAEAAAEVASLG"
     gene            complement(3003..3233)
                     /locus_tag="VIJ44_06295"
     CDS             complement(3003..3233)
                     /locus_tag="VIJ44_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165543.1"
                     /translation="MRSVRPLASVALVVTALLVGAAAPAGAQQLVDAGGDNGQGGLAF
                     VLMALMVFLIGFSLFFMDRVRRRRAAQQDEHR"
     gene            complement(3248..>3499)
                     /locus_tag="VIJ44_06300"
     CDS             complement(3248..>3499)
                     /locus_tag="VIJ44_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165544.1"
                     /translation="VPPSNLPFGVPPPAPTCDKTSPNNGFAVLTRDFGSLRIPPFVTF
                     VISVILFSLGLLALHWREKDERALAAAKAQTGALDPASS"
BASE COUNT          472 a         1325 c         1213 g          489 t
ORIGIN      
        1 gggagcaggc cagcgagctg cgcgcccacc tcacccaact gggcgggcgc gtcgtcgaac
       61 tccccaccat ccgcatcgag ccgctccccg tcgcgttgcc accactcgac gacgtcgact
      121 ggctcgtgct cacctcagcg aacggggtct ccgccctctt cgagcgggga atcacctccg
      181 ccggccgtga cgcccgggcc ctcgccgggg tccgtatcgc cgccatcgga cccggcaccg
      241 ccgccgccct cgcccgatac ggcgtgcggg cggacctcgt tcccgaccgg tatgtcgccg
      301 aggcgctcct cgctgcgttc ccgcccccga ccggcaccgg tgggcgcgtg ctcctggcgc
      361 gcgccgagca ggcccgagat ctcctccccg acggtctgcg caagctcggc tacgacgtcg
      421 acgtgctgcc gctgtaccgg acccgcgccg ccgaacccga cccggcgatc ctcgaccagg
      481 tccgccgcgg ccaggtcgac gccatcacgt tcacgtcgtc gtccaccgtc cgcaacttcg
      541 tcgagctcgt cgggaccctc gatccgcaac ccccggtggt gtcgatcggt ccgatcacct
      601 ccgccaccgc ccgcgagctc ggcctgcgcg tcgacgccga ggccgtcacc cacaccatcg
      661 acgggctcct caccgtgttg ctcgacaccc tcgcctgacc tccccgcacc ggcgagatcg
      721 ggccgccctc accgggtcgt acgatcgcgg ggtgcccttc ccggagcagc ggccccgccg
      781 gctgcgtcgc accagcgcct ggcggcgtct cgtcgccgag gcccgcgtcg gcatcgacga
      841 cctcgtcgcc cccctgttcg tcaaagaggg cctcaccgac cccgagccgg tcgcctccat
      901 gcctggtgtc gtccagcaca ctgtcgagag cctccgcaag gaggtccggg ccctcgccgg
      961 cctcgggatc cccgcggtgg tgctcttcgg tgtccccgcg accaaagacg cccgcggctc
     1021 gggtgctgac gaccccgacg gcgtgctggc cgtcgcgctc cgagcaatgc gcgacgaagt
     1081 cggcgacgac gtcgtcctcg tcgccgacac ctgcttggac gagtacaccg accacggaca
     1141 ctgcggcgtc ctcggccccg acggcaccgt cgacaacgac gcgaccctgg agcggtacgg
     1201 gtcgaccgcc gtcacccaag ccggcgccgg agccgacgtc gtggccccct cggggatgat
     1261 ggacggccaa gtcgccgcga tccgcgccgc gctcgacacc gccggacaga ccgacacggc
     1321 catcctcgcc tacgcggcca agtacgcgtc cgccctgtac gggccgttcc gtgacgccgc
     1381 ggagtccacc ccgagcttcg gtgaccgccg cgcctaccag atggatcccg ccaacggccg
     1441 cgaagccgag accgaggtgg cactcgacat cgccgaaggc gccgacctcg tcatggtgaa
     1501 gcccgccctc gcctacctcg acgtggtgag cagggtgcgg gccgcgaccg cagtgccact
     1561 cggggcgtac cacgtcagcg gggagtacgc catggtcgaa gctgcggccg cgaacggctg
     1621 gttcgacggc gacgccgtca tgctcgagca cctcaccgcg atcaaacgag ccggtgctga
     1681 cttcatcctc acctacgccg cccgtcgagt cgccgaacgt ctccagtgag cgacgagctc
     1741 tggacgcggg cgctcgcccg catccccggt ggtgtgaact cgccggtgcg gtcgttcgcg
     1801 agtgtcggcc gcgacccgtt cttcgtggct cgcggtgacg gcgcgtacct catcgccacc
     1861 gacgggacgc gatacgtgga ctacgtgcaa tcgtggggag cgtcgatcct cggccacgcc
     1921 caccccgcgg ttgtcgaggc cgtgcagcgc gccgccgccg cggggacgtc atacggggcg
     1981 ccgaccgcgg ccgaggtcga gctcgccgag gccgtcgccg accgggtggc gtcggtcgag
     2041 aagctccggc tggtgtcctc gggaaccgag gccgccatga ccgcggtgcg cctcgcccgc
     2101 ggggcgaccg gacggtccaa gatcgtgaag ttcgccggcg gctaccacgg ccacgtcgat
     2161 tcgctcctcg tcgccgccgg gagcggtgtc gccaccctgg ggatacccgg ctccgcgggg
     2221 atcaccgacg gcgcggtggc cgacaccctc gtcgtggcgt acaacgacga ggcggccctc
     2281 gacgcggtgt tcgacgagca cggggacgac ctcgccgcgg tcttggtgga gccggtcgcc
     2341 gcgaacatgg ggttggtgcc ccccgccccg gggttcctcg ccgggctgcg tcaccgctgc
     2401 gacctcgccg acgccctctt ggtgttcgac gaggtcatca cgggattccg ggtcggtcct
     2461 gccggcgccc aggggcggac cgggatcacg ccggacctga ccatgttcgg gaaggtcctc
     2521 ggtggcggct tgccgctcgc cgcgattggc ggtcccagcg cgctcctcga cgagctcgcg
     2581 ccggtcggac ccgtgtacca ggccggcacg ctctccggga acccgttggc gaccgcggcg
     2641 gggctcgcgg tgctcgcccg gctcgacgcg gccctgtacg acgacctgga gcggcgagct
     2701 ggtcgtctca ccgacgggct cgcgggcgca atccgcgacg cgggcttcgc ggtgcaggtc
     2761 ccgcgggcct ggacgctcgg tgggctgttc ttcgccgacg cgccggtgac cgattacgag
     2821 gcggctcgag ccgcggacgc gaagcgttac gcgcagttct tccaggcgat gctggcccgg
     2881 ggcgtattcc tggcgccgag cccgtttgag accttgttcc cgagcgtcgc gcacgacgac
     2941 gcggtgatcg accggaccgt ggccgccgcc gccgaggccg cggcggaggt cgcgagcttg
     3001 ggttagcggt gctcgtcctg ttgggcggcg cgtcggcgcc ggacccggtc catgaagaac
     3061 agggagaagc cgatcaggaa caccatgagc gccatcagca cgaacgcgag gccgccttgg
     3121 ccgttgtcgc caccggcgtc gacgagttgc tgggcgccgg caggagccgc ggcgccgacg
     3181 agcagcgcgg tgacgacgag tgccaccgag gccaggggtc gaacggaccg catcggcacc
     3241 atccttatca gctcgacgcc gggtcgagcg ccccggtctg ggccttggcg gcggccaggg
     3301 cccgctcgtc cttctcacgc cagtgcagcg ccaggaggcc gagggagaac aggatcaccg
     3361 agatcacgaa cgtcacgaag ggcgggatcc gcaacgagcc gaagtcgcgg gtgaggaccg
     3421 cgaacccgtt gttgggtgac gtcttgtcac acgtcggggc cggcgggggt accccgaacg
     3481 gcaggttgct cggtgggac
//
LOCUS       DATCCH010000332         3540 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_598801, whole genome shotgun sequence.
ACCESSION   DATCCH010000332 DATCCH010000000
VERSION     DATCCH010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598801"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2086)
                     /locus_tag="VIJ44_06305"
     CDS             complement(<1..2086)
                     /locus_tag="VIJ44_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165545.1"
                     /translation="MGERWSRLRSEMFGPASEEPYRRRTSDWIRVAAAIVLLFLAARH
                     AGSTSATEQAIFDFFNTLPNWLLPVFRNLYRLGALWAVGLVVVAALVGRRWRLARDLL
                     IAGILAWGLGRLLGQVVIEGASFAHGLRAAARLGSSPAFPAVRVAVLVAVMRAAAPYV
                     TRPTRRVGRVLIVGYVLAGMYLGTAFPNDVFGGLILGWAIAAAVHLAFGSPGGRPTSE
                     QVRRSLAELGIDATEIRLARRQPTGFTLMRARDATGDLRIKVIGRDEADAQFLAKLWR
                     FIVYRDSGPRLAWTRLQQVEHEAYLMLVAREQGVRVPSVVAAGTAGPRAALLVQRPIA
                     GVRLRDLAFDAVDQTLLEQVWEQVACLHASAHVVHGALDADHVVIDPEGACLVGFAAG
                     SSSGSAHQAAVDVAQLLTATAQIVGNERAVRAAAATLGADAVLTALPLLQPSVLPRRA
                     RHLLGVGHGTARQRLTQLRAVAGAVLDVEAPELVQLQRISGTSLAMAVGTLVAATVLL
                     ADVGDPTDVFGTFQHANGSWILVAFVLSFASNIGFAMGLQGTVPLRLPLWPTTELQVA
                     MSFSNLAVPGIGGTGMQVRYLQKQGVDLPSAIAAGGLLATVGNLVAAVGLFALAIAVE
                     PAHVNLALLPTSGLAELTLAAAAAVCVAAAVIGGIPRIRRTVMPPVIRAASTIWAALR
                     SPRQLALLIGGNV"
     gene            2474..3322
                     /locus_tag="VIJ44_06310"
     CDS             2474..3322
                     /locus_tag="VIJ44_06310"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5165546.1"
                     /translation="MSATFPLLGLEHLPDVVALARRSLRDPPTEQDLAVSLFTPGFPV
                     TVRGDPDTGVVASCVRDGAGYLRLLMVDPSARRQGLGDALLAAAEDDLAELPSITVGA
                     DAPDYLFPGVEIDHLEMLCLLERRRYDRGEANLNMVVDLDRLPPEPPGAGVARPGERD
                     ELEAWTAHHWPWWTTEVLRCADRDRLMVSRDDDGIAAVCCWDGARAGWVGPVAVRPSI
                     IGQGRGRSVLIAALHRMRTTGSTQAEIGWVGPIRPYVDTLGARMHRVFLVYRKTRRAD
                     TPRTGA"
     gene            3363..>3540
                     /locus_tag="VIJ44_06315"
     CDS             3363..>3540
                     /locus_tag="VIJ44_06315"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEY5165547.1"
                     /translation="MLAISGLLRFPLDARDEMVGVLTQLAERTRQDAGCIEYWWSEDL
                     SEPGAFRFFEAWESE"
BASE COUNT          552 a         1288 c         1225 g          475 t
ORIGIN      
        1 gcacgttgcc gccgatcaag agtgcgagct gtcgcggtga ccggagagcg gcccagatgg
       61 tcgacgcggc gcggatcacc ggcggcatga ccgtccgccg gattcggggg atgccgccaa
      121 tgaccgcggc ggcgacgcag accgcggcgg cggcggccag ggtcagctcc gcgagcccgc
      181 tcgtgggcag cagcgcaaga ttgacgtgcg cgggctccac cgcgatggcc aacgcgaaca
      241 gaccgaccgc cgcgaccagg ttcccgaccg tggcgagcag gcccccggcg gcgatcgcgg
      301 acgggaggtc gaccccctgc ttctgtaggt accgcacctg catcccggtg ccgccgatcc
      361 cgggtacggc caggttcgag aacgacatcg cgacctggag ctcggtggtg ggccacagcg
      421 ggagtcgcaa cggcaccgtg ccctgcaggc ccatggcgaa gccgatgttc gacgcgaacg
      481 acagcacgaa cgcgacgagg atccatgatc cattcgcgtg ctggaacgtc ccaaacacgt
      541 cggtggggtc tccgacgtcg gcgagcagca ccgtggccgc gacgagcgtg ccgaccgcca
      601 tcgccaggct ggtaccgctg atccgctgca gctgcacgag ctcgggagcc tcgacgtcga
      661 gcaccgcgcc ggcgaccgcg cggagctggg tgagtcgctg acgggccgtg ccgtgaccca
      721 cgcccaagag atgacgtgcc cggcggggca agaccgaagg ttgcagcagc ggcagcgcgg
      781 tcagcactgc gtcggcaccc agcgtcgcgg cggcagcgcg aacggcacgt tcgttgccga
      841 caatctgggc ggtcgcggtg agcagctggg cgacgtcgac ggcggcctgg tgggccgagc
      901 cactcgagct gcccgcggcg aacccgacca ggcacgcgcc ttccggatcg atgacgacat
      961 ggtcggcatc caacgcgccg tggacgacat gggcgctcgc gtggaggcag gcgacctgct
     1021 cccacacctg ctcgaggagc gtctggtcga ccgcgtcgaa ggcgaggtcg cggagccgga
     1081 ccccggctat cggacgctgg accagcagag ccgcgcgcgg cccggcggtg cccgccgcga
     1141 cgacggaggg gacgcggacg ccctgttccc gagcgacgag catgaggtag gcctcatgct
     1201 cgacctgctg taagcgggtc cacgccaggc ggggaccaga gtcgcggtac acgatgaacc
     1261 gccagagctt ggcgaggaac tgcgcgtccg cttcgtcgcg tccgatcacc ttgattcgca
     1321 ggtcgccggt ggcgtcgcgg gcgcgcatca acgtgaaacc agtcggctgg cgccgagcga
     1381 gccgaatctc ggtggcgtcg atcccaagct cggcgaggga tcggcggact tgctcggacg
     1441 tcggacgacc ccccggcgat ccgaaggcca ggtgcaccgc cgccgcaatc gcccaaccca
     1501 aaatcagacc gccgaacacg tcgttcggga aggcggtccc gagatacatc cccgccagca
     1561 cgtaaccgac gatcaggacg cgcccgactc ggcgcgtcgg tcgggtcaca tacggcgcgg
     1621 cggcgcgcat caccgcgacg agcaccgcga cgcggaccgc ggggaacgcc ggcgacgagc
     1681 ccagccgcgc agccgcccgc aggccgtggg cgaagctcgc gccctcgatg acgacttggc
     1741 cgagcaggcg ccccaacccc cacgcgagga tcccggcgat caacagatct cgggctaggc
     1801 gccaccgtcg gccgaccaac gccgcgacga cgacgagacc caccgcccac agggcgccga
     1861 gtcgatacag gttccggaac accggcagca gccagttcgg cagggtgttg aagaaatcga
     1921 agatcgcctg ctcggttgcg ctggtcgagc cggcgtgacg cgccgcgagg aacagcagca
     1981 cgatcgcggc cgcgacccgg atccagtcgc tggttcggcg gcggtatggc tcttcggacg
     2041 cgggcccgaa catctcgctg cgcagccgag accaccgctc gcccatgctc ctcaaccacc
     2101 ccagcggcct ggccacgccc ccacgctgac acgtccagcc tgctcgcgga cggtccgggg
     2161 cggtcaccgg gtgttccctc agcgtcagat cggttcaccc ggcgaggttt cgccgtcccg
     2221 gccaagtggg cgtggggcct ctgcctgcgg gcgaccgtga acgcccgatc gtccgaggcc
     2281 ggtcctaaga ccctcgaacc ccgagtggcg gctctccctc gcgcaacccc gaaccagcag
     2341 tagctcccga cccacgacga ccgcggcggg tccctcctgg cacgacggct ccgagcccga
     2401 ccacaatgcg ggccctcatg cctcgcccgt ggtgtcgacc gctcgactcc gtgcaacggg
     2461 ggtttccgac tcgttgagcg cgacgttccc gcttctcggg ctcgagcacc tcccggacgt
     2521 cgtcgctctc gctcgtcgtt ccctccgcga ccccccgacc gaacaggacc tggccgtgtc
     2581 gctgttcacg ccgggctttc ccgtgacggt ccgcggcgac cccgacaccg gcgtcgtggc
     2641 gtcctgtgtc cgtgatggag ccggctacct gcggctgctg atggtggacc cctccgcccg
     2701 acgccaaggg ctcggcgacg ccctgctggc cgccgcggag gacgacctcg cggagctccc
     2761 atccatcacc gtgggtgccg acgcccccga ctacctcttt cctggagtgg agatcgatca
     2821 cctcgagatg ctgtgcctgc tcgagcgtcg ccgctacgac cgcggtgaag cgaacctcaa
     2881 catggtcgtc gaccttgacc gcttgccgcc cgaacctccg ggtgccggcg ttgcccgtcc
     2941 cggcgaacgc gacgagctgg aggcgtggac cgcgcaccac tggccgtggt ggacgaccga
     3001 ggttcttcgc tgcgccgacc gggaccggct catggtgtcg cgcgacgacg acggaatcgc
     3061 ggccgtctgc tgctgggacg gggctcgcgc cgggtgggtc ggtccggtcg ccgtccgtcc
     3121 ctcgatcatc gggcagggcc gcggccgctc agtcctgatc gccgcgctgc accgaatgcg
     3181 caccaccggg tcgacccagg ccgagatcgg atgggtgggt ccgatccgcc cgtacgtcga
     3241 cacgctcggc gcccggatgc accgcgtctt cctcgtctac cgcaagaccc gtcgagcgga
     3301 cacgccgaga accggcgcgt agcctcgccc cagctcggat gccgtcgtcg gaagggccat
     3361 cgatgctcgc gatctcgggg ctgctccggt tcccgctcga cgcgcgtgac gagatggtcg
     3421 gggtcctcac ccagctggcc gagcggaccc gccaagatgc cggctgcatc gagtactggt
     3481 ggtccgaaga tctgtcggaa cccggcgcct tccgcttctt tgaggcctgg gagtcagagg
//
LOCUS       DATCCH010000333         2204 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_493434, whole genome shotgun sequence.
ACCESSION   DATCCH010000333 DATCCH010000000
VERSION     DATCCH010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493434"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="VIJ44_06320"
     CDS             <1..622
                     /locus_tag="VIJ44_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEY5165548.1"
                     /translation="LLALGKLYEAIPIHEQVLPVVEQVLDPGCLVSSLSSITILPDET
                     AQPIHADDQLIPLPKPHVPTVCNTMWALTDFTEANGATRVIPGSHTADGSPDFGAPYD
                     SVPAEMPKGSVLVWHGSLWHGGGANQTEKPRVGIAMNYCAGWIRQQENQQLGIPRDIA
                     TGFSPRLRRLVGYGIYSGLIGHIDKHDPVEMLGDAPADDAMVWDQI"
     gene            complement(643..1410)
                     /locus_tag="VIJ44_06325"
     CDS             complement(643..1410)
                     /locus_tag="VIJ44_06325"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004977045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEY5165549.1"
                     /translation="MGGGSGVEDKVVLVTGGASGLGEAAVGLLAAGGARVVVADLDAA
                     RGDAVVAAARAAGAEAMFCRTDVTQEPDVAAAVAAALETWGRLDGAINNAGTTGPSNP
                     TAEYSVEDWNRVLALNLTGVFLGLKHEIPPMVEQGSGSIVNTASGAGLVGFAGLPAYV
                     ASKHGVVGLTRAAALEYVRAGVRINAVCPGSTRTPMLEGFMGGDPTIERMMAQSAPIG
                     RLARPAEIAEAMVWLLSDAASFVVGHALAVDGGAVVA"
     gene            1493..>2204
                     /locus_tag="VIJ44_06330"
     CDS             1493..>2204
                     /locus_tag="VIJ44_06330"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY5165550.1"
                     /translation="MAPTVERNVQDRNSPKDVGRNLRHVRKQQGLSRGAAARSAGLTR
                     RELAHYERGKVEVPESDLWCLAGSCGVDVSELLPRREPLRVSPDLSTISTGDTIRHLR
                     GPAEPDGLLREYLAMIYELRNLPLGSPVPLRQPDLTALADALGGTPDSIEHRLIEIIG
                     ASPEEAARIRAMILLPLSLPSAAETPRVDPYAALATPNTPPAVEDFFASPPATDLLPA
                     PSPAATASPFAAPTGAPRP"
BASE COUNT          337 a          887 c          687 g          293 t
ORIGIN      
        1 cctgctggcc ctcggcaagc tctacgaagc gatcccgatc cacgaacagg tcctgccggt
       61 cgtcgagcag gtcctcgacc ccgggtgcct cgtgtcctca ctctcgtcga tcacgatcct
      121 ccccgatgag accgcgcagc ccatccatgc cgacgatcag ctgatcccgc tccccaaacc
      181 ccacgtcccg acggtgtgca acaccatgtg ggccctgacc gacttcaccg aggcgaacgg
      241 cgcgacgcgg gtcattcccg gatcgcacac cgctgacggc tcccccgact tcggcgcccc
      301 ctacgattca gtcccggccg agatgccgaa aggcagcgtg ctcgtctggc acggcagcct
      361 ctggcacgga ggcggcgcga accaaaccga gaagccgcgg gtcgggatcg cgatgaacta
      421 ctgcgccggg tggatacgcc aacaggagaa ccagcagctc ggcatcccac gcgacatcgc
      481 caccggcttc tcgcctcgcc tgcggcgact cgtcggctac ggcatctact ccgggctcat
      541 cggtcacatc gacaagcacg acccggtcga aatgctcggc gacgctcctg ccgacgacgc
      601 gatggtctgg gaccaaatct gatcccacgc tcgtcgcgcc cctcacgcga cgacggcgcc
      661 gccgtccacc gcgagcgcgt gcccgaccac gaacgacgcg gcgtcggata ggagccacac
      721 catcgcctcg gcgatctccg ccgggcgcgc caggcgaccg atcggcgccg actgcgccat
      781 catccgctcg atggtcgggt cgccgcccat gaacccctcg agcatcgggg tgcgggtgct
      841 acccgggcac acggcgttga tccgaacccc ggcgcggacg tactcgagcg ccgcggcccg
      901 ggtcagcccc accacgccgt gtttgctcgc cacgtacgcg ggaaggccgg cgaagccgac
      961 gagccccgca cccgaggccg tgttcacgat cgacccactc ccctgctcga ccatcggcgg
     1021 gatctcgtgc ttcaggccga ggaacacccc ggtcaggttc agggccagca cccggttcca
     1081 gtcctcgacc gagtactcag ccgtcgggtt cgatggcccg gtcgtcccgg cgttgttgat
     1141 cgcgccgtcg agccggcccc aggtctcgag ggccgcggca acggccgcgg ccacgtcagg
     1201 ctcctgggtc acatcggtcc gacagaacat cgcctccgcc ccggccgccc gggcggcggc
     1261 gaccaccgcg tcgccgcggg cggcgtcgag gtccgcgacg acaacccggg ccccacccgc
     1321 ggccaacaga cccaccgcgg cctcgccgag gcccgacgct ccgccggtca ccagcaccac
     1381 cttgtcctcg acgccggatc cgccacccac gggcactccc tccgttgggg tccatttggg
     1441 gtccggacca taggtccttt agccgtgcga ctggcgcgcc gataccaggg ccatggcgcc
     1501 gaccgttgag cgcaacgtcc aggaccggaa ctcgccgaag gacgtcgggc gcaacctccg
     1561 ccacgtccgc aagcaacagg ggctctcacg cggcgcggcg gcccgatccg ccgggctgac
     1621 ccgacgagag ctggcccatt acgagcgcgg caaggtcgag gtccccgaga gcgacctgtg
     1681 gtgcctcgcc ggatcgtgcg gcgtcgacgt ctccgagctg ctcccgcgcc gggaaccgct
     1741 gcgcgtcagc cccgacctgt ccaccatctc gaccggcgac accatccgcc atctacgcgg
     1801 cccggcggag cccgacgggt tgctgcgcga gtacctggcc atgatctacg agctgcggaa
     1861 ccttcctctg ggcagtccgg tgccgctccg ccagcccgac ctgaccgccc tcgccgacgc
     1921 cctcggtggc acgccggaca gcatcgagca tcggctgatc gagatcatcg gcgcctcacc
     1981 ggaggaggcc gctcgcatcc gggccatgat ccttctgccg ctgtcgctgc ccagcgcggc
     2041 cgagacccca cgggtggacc cgtacgcggc gctcgccacg cccaacacac cgcccgcggt
     2101 ggaggacttc ttcgccagcc cgccggccac cgacctcctc ccggccccgt cgcccgccgc
     2161 gaccgcttcg ccgttcgcgg cgccgaccgg cgcgccgagg ccgt
//
LOCUS       DATCCH010000334         2633 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_225301, whole genome shotgun sequence.
ACCESSION   DATCCH010000334 DATCCH010000000
VERSION     DATCCH010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225301"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VIJ44_06335"
     CDS             complement(<1..282)
                     /locus_tag="VIJ44_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019046559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5165551.1"
                     /translation="MPEPSVSLAGRVAIVTGASRGIGRGLALGLAAAGAAVVCAARSE
                     TEAPGGLPGTIHATAEAIRAAGGVAVAQRCDIGVEHDIVSLIDATRSEFG"
     gene            complement(312..1379)
                     /locus_tag="VIJ44_06340"
     CDS             complement(312..1379)
                     /locus_tag="VIJ44_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_255838054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(1029..1031),aa:Sec)
                     /transl_table=11
                     /product="ResA-like WAxxUGC motif-containing protein"
                     /protein_id="HEY5165552.1"
                     /translation="MILTDLHVEPATFAAETGWVIKPEGACRADVCVPLPSDARIPDD
                     RLDVRVVAERLGMPLVHDEPHRFWALGPETHTGRALTSAHAPPLELPDVDGNPFQLSS
                     LLGQKVLLVAWASWUGCRFDLPVWQELRTRLHPQGLEIVTVALDVDVEAARPFVEAAN
                     PSHPSLIDPAHRVDELFGFVNVPNGVWIDEDGMIVRPAEPAFPGRNPILESLQQIDLD
                     TLPPEAAEMLGEARKIKTDPEAYLAMILDWVEHGPASRYALAPDEVLRRAQPRPADEA
                     LAAAHFEAGEELHRSGEHGAAIPHWREAHRLFPTNWTYRRQAWELEDPSRQGHTDAYD
                     SSWFEDVKKVGAENYYPPVIP"
     gene            complement(1446..1844)
                     /locus_tag="VIJ44_06345"
     CDS             complement(1446..1844)
                     /locus_tag="VIJ44_06345"
                     /inference="COORDINATES: protein motif:HMM:NF017774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEY5165553.1"
                     /translation="MAHLVVDGMNLIGTRPDGWWRDRPGATRRLLARLQALHRATGET
                     VTVVLDGRPLPDVPEGTHHGVRVLYARRGGRDAADDRLVEHLGELDDPTEVTVVTSDR
                     GLRVRALELGATVTGAGSLLARLDQLTDST"
     gene            complement(1974..>2633)
                     /locus_tag="VIJ44_06350"
     CDS             complement(1974..>2633)
                     /locus_tag="VIJ44_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEY5165554.1"
                     /translation="SLTLLSRSMRSVQAIGGVCRSGASATTIVYHCPSGVAGTTTLSI
                     FGGLLFLLLFVICAGETGRSLLLLAWSALFLTLGWDFLDYGFHLTSGSGTNGGFIVCA
                     VLFIVMGAVPLFWLLPRLWSVITGTPDPSDAPGAPGHVPLVGATSVQFTPPPSSSPPA
                     WGTGTSVRMASPPSNAPSTGKDVAGELERLASLHRRGELTDAEYEAAKAQAIANTGKP
                     T"
BASE COUNT          411 a          873 c          969 g          380 t
ORIGIN      
        1 cccgaactcc gaccgggtgg cgtcgatcaa cgacacgatg tcgtgttcga ccccgatgtc
       61 gcatcgctgg gcgacggcga cgccacccgc ggctcggatg gcctcggcgg tcgcatggat
      121 cgtgcccggg agcccaccgg gggcttcggt ctcgctgcga gctgcgcaga ccaccgcggc
      181 gcccgccgcg gcgaggccga gggcgaggcc ccggccgatt ccccgcgacg ccccggtgac
      241 gatggcgacg cggccggcca gcgaaaccga cggttctggc atcaggcggc gctcacgacg
      301 gggtctggga actaggggat gaccgggggg tagtagttct cggcgccgac cttcttgacg
      361 tcttcgaacc acgagctgtc gtaggcgtcg gtgtgtcctt gccggctggg gtcttcgagc
      421 tcccaggcct ggcggcggta ggtccagttg gtcgggaaga ggcgatgggc ttcgcgccag
      481 tgcgggatcg cggcgccgtg ctcgccggac cggtggagct cctcaccggc ttcgaagtgc
      541 gccgcggcga gggcctcgtc ggctggtcgg ggctgggctc ggcggagcac ctcgtccggg
      601 gcgagggcgt agcggctggc cggcccgtgt tccacccagt cgaggatcat cgccaggtaa
      661 gcctcggggt cggtcttgat cttgcgggcc tccccgagca tctcggcggc ctccggcggc
      721 agcgtgtcga ggtcgatctg ctgcagcgat tcgaggatgg ggttccgtcc ggggaacgcc
      781 ggctcggccg gtcgcacgat catcccgtcc tcgtcgatcc agactccgtt cgggacgttg
      841 acgaaaccga acagctcgtc tacccggtgg gctgggtcga tcagcgatgg gtgcgaaggg
      901 ttcgcggctt cgacgaacgg ccgggccgct tcgacgtcga cgtccagagc aacggtgacg
      961 atctcgagac cctgcgggtg gaggcgagtg cgcagctcct gccacacggg caggtcgaaa
     1021 cgacatcctc accaagatgc ccacgcgacg agcaggacct tctggcccag caggctggac
     1081 agctggaacg ggttgccgtc gacgtcgggc agctcgaggg gtggagcgtg ggcgctggtc
     1141 agcgcccgcc cggtgtgcgt ctcgggtccg agagcccaga accggtgcgg ttcgtcgtgg
     1201 acgagcggca tcccgagccg ttctgcgacg acgcgcacgt cgaggcggtc gtcggggatg
     1261 cgggcgtcgc tcggcagcgg cacgcagaca tcagcgcggc aggccccttc gggcttgatg
     1321 acccatccgg tctcggcggc aaaggtcgcc ggttcgacgt gaagatcagt gagaatcatc
     1381 ggagagctcc tcggattggc cgctctgtcg aggagactac gagcaaactc gtcgacggcg
     1441 cgacgtcacg tcgaatcggt gagctggtcc aaccgcgcca ggaggctccc ggcgccggtc
     1501 accgtcgcgc cgagctcgag ggcccggacc cgcagccccc ggtccgacgt gacgaccgtg
     1561 acctcggtgg ggtcgtcgag ctcgcccagg tgttcgacca ggcggtcgtc ggcggcatcg
     1621 cgcccacccc gccgcgcgta gaggacccga acgccgtggt gggtgccctc gggtacatcg
     1681 ggaagcggtc ggccgtcgag cacgaccgtc acggtttcgc ccgtggcccg gtggagtgct
     1741 tggagacgag ccaggagccg ccgggtcgca ccggggcggt cacgccacca gccgtcaggg
     1801 cgggtcccaa tcaggttcat cccgtcgacg accaggtgtg ccatcggtcc ccgctccggg
     1861 tggtcggacg cgacctcggt cggcctactt cgtgacgagg tcgaaaacct ggaagccgcc
     1921 ccagacgccg atcgcgatcg ccgcgacgta cgtcaaaccg agcacaaagc gagtcatgtt
     1981 ggcttccccg tgttcgcgat ggcctgagcc ttggcggcct catactcggc gtcggtgagc
     2041 tcaccccgcc ggtgcagcga ggcgagacgt tcgagctccc cggccacgtc cttgcccgta
     2101 ctcggggcgt tcgagggtgg cgaggccatc cggacgctgg tccccgtccc ccaggcgggc
     2161 gggctcgacg agggcggcgg ggtgaactgg acgctggtcg cacccacaag cgggacgtga
     2221 ccgggcgcac cgggagcgtc ggacggatcg ggggtgccgg tgatgacgct ccacaaccgg
     2281 gggagcagcc agaagagcgg caccgctccc atcacaatga acaggaccgc gcagacgatg
     2341 aacccgccgt tggttccgct cccgctggtc aggtgaaatc cgtagtcgag gaagtcccac
     2401 ccgagggtga ggaacagcgc cgaccatgcc aggaggagca gcgagcggcc ggtctcgccg
     2461 gcgcagatca cgaagagcag aaggaacagc agcccgccga agatcgacag ggtcgtcgtg
     2521 ccagccacgc ccgaagggca gtgatagacg atcgtcgtcg cgctggcacc cgagcggcag
     2581 acgccgccga tcgcctggac cgagcgcatc gaccgggaca gcagcgtcaa cga
//
LOCUS       DATCCH010000335         2871 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_534513, whole genome shotgun sequence.
ACCESSION   DATCCH010000335 DATCCH010000000
VERSION     DATCCH010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534513"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VIJ44_06355"
     CDS             <1..312
                     /locus_tag="VIJ44_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007459909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HEY5165555.1"
                     /translation="GRTPPPGHSLHVDHQHETGVVRGLLCFRCNAGIGQFREDKLRLA
                     DAIVYLARGRHALEPDRGERELFVELVCELVGAKCVDAERSRVIGIDDSAPIGAEQTP
                     E"
     gene            325..1137
                     /gene="prcB"
                     /locus_tag="VIJ44_06360"
     CDS             325..1137
                     /gene="prcB"
                     /locus_tag="VIJ44_06360"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HEY5165556.1"
                     /translation="MASESRLPFFSSEADPGPSFVDVLRRLQPMSGGGLASGSAASLE
                     APHGTTVLAIRYADGVVMAGDRRATAGFTIASRRIEKVFPADDSSGVAIAGAAGPAVD
                     LVRLFQTQLEHYEKIEGEILSLEGKANQLSQLVRANLPAAMQGFVVVPLFAGFDDRRD
                     LGRVFSFDVTGGKYEEIDFQAQGSGGVHARNWIKSQWRADLTRDEAVDLALRALFAAA
                     DEDAATGGPDLVRRIFPTVAVIDASGFTAVDDDEVARRADELIGGREEGAGL"
     gene            1140..1820
                     /gene="prcA"
                     /locus_tag="VIJ44_06365"
     CDS             1140..1820
                     /gene="prcA"
                     /locus_tag="VIJ44_06365"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HEY5165557.1"
                     /translation="MPFYVPPEQMMKDRADYARKGIARGRSLIAMSAAEGIVLIAENP
                     SRTLYKISEIYDRIAFAGVGKYNEFEMLRIAGVRQADVKGYSFAREDVNARSLANAYA
                     QTLGQVFTHELKPYEVEILVAQVGATPNDDELFQILYDGVVNDKEGYTVLGGQAETVT
                     TALEERYSTDLDEGAAVRLGAAVLAAGGAEPLTANQLEVALLDRTKTRRAFHRVKGAE
                     LEALLSGG"
     gene            complement(1824..2162)
                     /locus_tag="VIJ44_06370"
     CDS             complement(1824..2162)
                     /locus_tag="VIJ44_06370"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HEY5165558.1"
                     /translation="MRSLTWVSGSFSAHLLAARLHSEGIDVQLRGALDSPYGLTMGEM
                     ARVDVFVPEDQLADAELVMLATEVDQVLAPPRDWAGDGAATGRRRWALWVALGLLALV
                     SLAPLLRLLS"
     gene            2337..2858
                     /locus_tag="VIJ44_06375"
     CDS             2337..2858
                     /locus_tag="VIJ44_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165559.1"
                     /translation="MTAAALVVSMAGVAAAKPQKQSPAKYAKTVCGAYTSFTDMAKSY
                     ETALSAIDPSDPAAYTTQAVAATNTFLTAFQTSVSKLTNAYPDVSDGKKVATLLNASA
                     TTIQSSVKTAIANLQAATSPGAVAVAQFSAAVTTLFATLIANDPFSKITDQTVLGALK
                     KEKSCKNVVQIIG"
BASE COUNT          517 a          920 c          976 g          458 t
ORIGIN      
        1 ggccggacgc caccgcccgg gcactcgctg catgtggacc atcagcatga gacgggagtc
       61 gttcgcgggc tgctctgctt ccggtgcaac gcggggattg gacagttccg cgaggacaag
      121 ctccgtcttg ctgacgccat cgtctacctg gcccgtggac gccatgccct cgagcccgac
      181 cgtggcgaac gcgagctgtt cgtcgagctc gtctgcgagc tggtgggcgc gaagtgcgtt
      241 gatgccgagc ggtcgcgagt catcggcatc gacgactccg cgcccatcgg agccgaacag
      301 acgcccgagt aggctcgctt ctcgatggct agcgagtcgc ggttgccgtt cttctccagt
      361 gaggccgatc ccggaccgtc gtttgttgac gtgttgcgcc gcctccagcc gatgagcggc
      421 ggtggattgg cgtcggggtc ggcagccagt cttgaagctc cccacggcac gaccgtcctc
      481 gcgattcggt acgccgacgg agtggtcatg gccggtgacc gacgagcaac cgccgggttc
      541 acgatcgcca gccgccggat cgagaaggtg ttcccggcag acgacagctc cggggtggcc
      601 atcgcgggcg cggccggccc agccgtagat ctggtgcggc tgtttcagac gcagttggag
      661 cactacgaga agatcgaagg ggagattctc tccctcgagg ggaaggcgaa ccagctcagc
      721 cagctggtgc gcgccaatct ccccgcggcg atgcagggat ttgtggtggt cccgttgttc
      781 gccgggtttg acgaccgtcg agatctcggc cgggtgttca gcttcgatgt gaccggtggc
      841 aagtacgaag agatcgactt tcaggcgcaa ggttcgggtg gtgtgcacgc acgcaactgg
      901 atcaagtcgc agtggcgcgc agacctgacc cgtgatgaag ccgttgacct cgcgctgcgc
      961 gcgttgttcg ccgccgcaga cgaggacgcg gcgacgggcg gaccggacct agtgcgccgc
     1021 atcttcccaa cggtggcggt catcgacgcg tcggggttca ccgcggtcga cgatgacgag
     1081 gtcgcgcgtc gggctgacga gctgatcggc ggacgagagg aaggcgcagg actatgacga
     1141 tgcccttcta cgtgccgccc gagcagatga tgaaagaccg cgcggactat gcccgcaagg
     1201 gaatcgcccg tggtcgcagc ttgatcgcca tgagcgcggc cgaagggatc gtgctcatcg
     1261 ccgagaatcc gtcccgcacc ctgtacaaga tcagtgagat ctatgaccgc atcgccttcg
     1321 cgggcgtcgg caagtacaac gagttcgaga tgctccgcat cgccggtgtg cgtcaggccg
     1381 acgtcaaggg ctattcgttc gcccgcgagg acgtcaacgc ccggtcgttg gcgaacgcgt
     1441 acgcccaaac cctcggccag gtgttcacgc acgagctcaa gccctacgag gtcgagatcc
     1501 tcgtcgccca ggtgggcgcg acccccaacg acgacgagct cttccagatt ctctacgacg
     1561 gtgtggtgaa cgacaaggag ggctacaccg tcctcggggg ccaggccgag acggtgacga
     1621 ctgcgctcga ggagcgctac tcgaccgacc tcgacgaagg agccgcggtg cgtctgggcg
     1681 cggcggtcct cgccgccgga ggcgccgagc cgctgacggc caatcagctc gaggtcgcgc
     1741 tcctcgaccg caccaagacc cgccgggcgt tccaccgggt caaaggcgcc gagctcgagg
     1801 cgctcctcag cggcggctga ccgtcacgag agcagtcgca gtagcggcgc gaggctgacg
     1861 agcgccaaga gcccgagggc cacccacagc gcccaccgcc ggcgaccggt cgcggcgccg
     1921 tccccagccc aatctcgagg cggggccagg acctgatcga cctcagtggc gagcatgacg
     1981 agctcggcgt ctgcgagctg gtcctccggg acgaagacat cgacccgggc catttcgccc
     2041 atcgtgagcc cataggggct gtcgagggcg cctcggagct gcacgtcgat cccttccgag
     2101 tgcaggcgag ccgcaagcag atgggccgag aacgaaccgc tgacccacgt cagtgaacgc
     2161 atgggctcca ccagcgaagc ctatgaccgg cggtgggcgg aggcaccggc tggtctcggc
     2221 ggaaatgagt accgcctact cacgctgggt ggccggcggt gttctagaaa gcggggagtt
     2281 cgagtcgacg aaggggcagc aatatgaggg cagtgatccg tggcggttgc gtcgcggtga
     2341 ccgcggccgc gctggtggtg agcatggcgg gcgtcgccgc tgcgaagccc caaaagcagt
     2401 cccctgcgaa gtacgccaag accgtgtgtg gcgcctatac gagctttacg gacatggcga
     2461 agagctatga gacggccctg agcgctatcg atccctccga cccggccgct tacacgacgc
     2521 aggcggtggc tgccacgaac accttcctga cggcatttca gacgtcggtg tccaaactga
     2581 ccaatgcata tcccgacgtc tcggacggga agaaggtggc gacgctcctg aatgcgagtg
     2641 cgacgacgat ccagtcgtcc gtgaagactg cgatcgcgaa tctccaggcc gcaacgtctc
     2701 ccggtgccgt cgcggtcgcc cagttctcgg cagcggtcac aacactattc gcgaccctga
     2761 tcgccaacga cccgttctcc aagatcacgg accagaccgt tctgggcgcc ttgaaaaagg
     2821 aaaagtcgtg caagaacgtc gttcagatca tcggctgacg ggctgacacc c
//
LOCUS       DATCCH010000336         5858 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_326314, whole genome shotgun sequence.
ACCESSION   DATCCH010000336 DATCCH010000000
VERSION     DATCCH010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5858
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326314"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="VIJ44_06380"
     CDS             <1..818
                     /locus_tag="VIJ44_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012947828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HEY5165560.1"
                     /translation="PVTVRLLDPPLHEFLPNIEELLVQDARGELDDEGRRLLHAAQQW
                     REANPMLGTRGVRLGILKPGLYRTQVRALFDAAMDRKLAGGDPHIEVMIPLAVNRPEL
                     ALAISWVREVAEEIASATYGAGGVNYTVGTMVETPRAALVADEIAHEAEFFSFGTNDL
                     TQMTFGFSRDDIEGRFLPRYLELNLLPANPFETLDIDGVGKLVRMAVELGRKTRPELK
                     LGICGEHGGDPASVDFCHEVGLDYVSCSPYRVPLARLAAAHAALAAAGPGSTA"
     gene            815..1258
                     /locus_tag="VIJ44_06385"
     CDS             815..1258
                     /locus_tag="VIJ44_06385"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEY5165561.1"
                     /translation="MSTPALNAREITDFLEEIWPGVTGSITVEAVSDRHARVRSIVTA
                     DRLRPGGIVSGPVLMALADTAVWVAVLSVVGRVAMTVTVDLNIHFLRPAPHADLVAEA
                     RLLKIGHRLAVGDVLIYSDHDPDPVAHATVTYAIPPRHDESDRHT"
     gene            1275..2384
                     /locus_tag="VIJ44_06390"
     CDS             1275..2384
                     /locus_tag="VIJ44_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY5165562.1"
                     /translation="MELAPILRTNERFNGHGDGVTQAHPGVQAYVGRAARSREEREAD
                     LDAALAPGATRPVGAGHRTRPEAPDPYRLCFERDLDRVKHSRPWRRLAGKCQVFIAPE
                     DDHLRTRLTHAVEVAQVATGIARAVDLCLPLTEAIALAHDCGHGPAGHASEEAFSPYL
                     PATGYDHAVYGADVTLAPLNLCHETLDGIRNHSWRRPPPTTPEGEVVAWADRIAYVCH
                     DFQDAVRAGILTPDDLPDEVRGVVGARSSDQIESFVLAVLDAIGRTGHVGMTEPAGSA
                     LLTFRDFNFERIYLRPAARRQAERVIRLLRGLVDSFADAPRRLPEPDGADVEPGSSAA
                     TALAVRYVSGMTDRYALGLGVELLGWRPEDLPRGV"
     gene            2431..4221
                     /gene="dnaG"
                     /locus_tag="VIJ44_06395"
     CDS             2431..4221
                     /gene="dnaG"
                     /locus_tag="VIJ44_06395"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEY5165563.1"
                     /translation="MGILDEDVAHVRETTDLVAVAGDHIALKRVGQRWMGLCPFHQEK
                     SPSFSINPALNLFYCFGCQKSGDAITFVREVEHLDFVEAVERLAQRAGITLRYDDRRT
                     GQDRSRKTRLAEAVGAAIDFYHRRLNESPDGGAARKYLRGRGFDGDAARRFQLGWAPD
                     DWDLLSRHLDQRGFARDDIEGAGLAFVNRANRLQDQFRSRLMFPIYDARGEPAGFGGR
                     TLGDDQPKYKNSPETAIYHKSRLLYGLNWAKGEIVARGDVIICEGYTDVMGFALAGAP
                     NAVATCGTALADDHFQVLKNLARRVTLAYDADAAGQGAAERWYRWEQEYEIQLRVADL
                     PPGHDPGDLWQHDQPRLLLALEHAEPFLQFRLDRAIAGADLATIEGRARAATTAATII
                     AEHPNELVRDQYVMKLAGALDIDADRLRDAVTQARRAPRRPSREPPSDTPPPEPRRRP
                     NVDRRELDLLRWAIHEPELIADWLNETLLQDPLARAAYHRLANSPSFADALDGTDDDV
                     RALLERLAVEEPEADDEPETLRARLLVINVEPVAKRTLTTLLAAGDEERAGALKRNLD
                     ELVHAREIGEWERAEELGRRTLQWIVSSPE"
     gene            4232..5560
                     /gene="rpoD"
                     /locus_tag="VIJ44_06400"
     CDS             4232..5560
                     /gene="rpoD"
                     /locus_tag="VIJ44_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003150470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEY5165564.1"
                     /translation="MSQPNNAAALTPVDPAELDQVIEELVIRARERGSVTSGEIFAAL
                     HTLEPSTEELAEIYRRIEARGIAIQDEITEELELEDARRRDSPATNHRGPRGEPTRAT
                     TAVRPGDAVTTRPARVRRDLASVFDEPAARSDRGETGSFDPVRMYLKEIGKVPLLTAP
                     QEVNLAKRIEAGVLAEERLESHPEMTAGQEASEHANVEDGELAKQQLTEANLRLVVSI
                     AKRYVGRGMALLDLVQEGNLGLMRAVEKFDYTKGFKFSTYATWWIRQAITRAIADQAR
                     TIRIPVHMVETMNKVLRQQRQMLQELGREPTVDEVARKVDLTSDKVREIQRISQEPLS
                     LETPVGEEDDSSLGDFVSDPTAPEPTEEAEQQGLKEEITRALEELNDREREIMRLRFG
                     LDDGQPHTLEEVGRRFKVTRERIRQIESKTLAKLRHPTRSQRLRAYLDGA"
     gene            5580..5652
                     /locus_tag="VIJ44_06405"
     tRNA            5580..5652
                     /locus_tag="VIJ44_06405"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5613..5615,aa:Ile,seq:cat)
BASE COUNT         1036 a         2404 c         1651 g          767 t
ORIGIN      
        1 tacccgtcac cgtccggctc ctcgacccgc cgctgcacga gttcctcccc aacatcgaag
       61 agctcctcgt ccaagacgcc cgcggcgagc tcgacgacga aggccggcgg ctcctccacg
      121 ccgcccagca atggcgcgaa gccaacccga tgctcggcac ccgcggcgtc cgcctcggca
      181 tcctcaaacc gggcctctac cgcacccagg tccgggccct cttcgacgcg gccatggacc
      241 gcaagctcgc cggcggcgac ccccacatcg aggtcatgat ccccctcgcc gtcaaccgcc
      301 ccgagctcgc cctcgcaatc tcttgggtcc gcgaagtcgc agaggagatc gccagcgcca
      361 cctacggcgc cggcggcgtc aactacaccg tcggtaccat ggtcgagacc ccccgcgccg
      421 ccctcgtcgc cgacgagatc gcccacgagg ccgagttctt ctccttcggc accaacgacc
      481 tcacccagat gaccttcggg ttctcccgcg acgacatcga aggacggttc ctgccccgct
      541 acctcgagct gaacctcctc cccgccaacc cgttcgagac cctcgacatc gacggcgttg
      601 gcaagctcgt gcgcatggcc gtcgagctcg gccgaaaaac ccgacccgaa ctcaagctcg
      661 ggatctgtgg cgaacacggc ggcgatcccg cctccgtcga cttctgtcac gaggtcggcc
      721 tcgactacgt ctcctgctct ccgtaccggg tgccactggc ccggctcgcc gccgcccacg
      781 ccgcgctcgc cgcggcggga cccggctcca cggcgtgagc acccccgcgc tgaacgcgcg
      841 ggagatcacc gacttcctcg aggagatctg gcccggtgtg accggatcga tcacggtcga
      901 agcggtcagc gaccgccacg cccgggtccg cagcatcgtc accgccgacc ggctccgccc
      961 cggcgggatc gtcagcgggc ccgtcctcat ggctctcgcc gacaccgcgg tctgggtcgc
     1021 ggtcctctcc gtcgtcggcc gcgtcgccat gaccgtcacc gtcgacctca acatccactt
     1081 ccttcgtccc gcgccccacg ctgatctcgt cgccgaggcg cgactcctca agatcggaca
     1141 ccgcctcgcc gtcggcgacg tcctcatcta cagcgaccac gaccccgacc ccgtcgcgca
     1201 cgccaccgtc acctacgcca tcccacctcg tcacgatgag tcggatcgtc acacctgacc
     1261 ggtaccctcg agccatggag ctcgcaccca tcctccggac gaacgaacgg ttcaacgggc
     1321 atggcgatgg cgtcacccag gcccaccccg gcgtccaggc gtacgtcggc cgggccgccc
     1381 gctcccgcga ggaacgtgaa gccgacctcg acgccgcgct cgcccccggc gcaacccgac
     1441 cggtcggggc cgggcaccgc acccgccccg aggcgcccga cccctaccgg ctctgcttcg
     1501 aacgcgacct tgaccgcgtc aagcactccc gaccctggcg tcgtctcgcc ggcaaatgcc
     1561 aggtgttcat cgcccccgaa gacgaccacc tccgcacccg cctcacccac gccgtcgaag
     1621 tcgcccaggt cgccaccggg atcgcccgcg ccgtcgacct gtgccttccc ctcaccgaag
     1681 ccatcgcgtt ggcccatgac tgtggtcacg gtcccgctgg tcacgcgtcc gaagaggcgt
     1741 tctccccgta cctgcccgcc accggctacg accacgccgt ctacggcgcc gacgtcaccc
     1801 tcgcgcccct caacctgtgc cacgagaccc tcgacgggat ccgcaaccac tcctggcgcc
     1861 gcccaccccc caccaccccc gaaggcgagg tcgtcgcctg ggccgaccgg atcgcctacg
     1921 tctgtcatga cttccaagac gctgtccgcg ctgggatcct cacccccgac gacctccccg
     1981 acgaggtccg cggcgtcgtc ggtgcccgca gctccgacca gatcgagtcg ttcgtgctcg
     2041 ccgtcctcga cgccatcggc cggaccggcc acgtcggcat gaccgaaccc gcgggatcgg
     2101 cactgctcac cttccgggac ttcaacttcg agcggatcta cctgcggcct gcggcccgcc
     2161 gccaagccga gcgggtcatc cgcctccttc gcggcctcgt cgacagtttc gccgacgcgc
     2221 cgcgacgcct ccccgaaccc gacggcgccg acgtcgagcc cggctcgagc gctgccaccg
     2281 ccctcgctgt ccggtacgtc agcggcatga ccgaccggta cgcgctcggt ctcggcgtcg
     2341 agctcctcgg gtggcgtccc gaagacctcc cccgcggggt ctgatctgag cgccccgggg
     2401 cgccgcaccc gcgccgtaac ctgacccgcg atgggcatcc tcgacgagga cgtcgcgcac
     2461 gtccgcgaga ccaccgacct tgtcgcggtc gccggcgacc acatcgcgct caaacgcgtc
     2521 ggtcaacgat ggatgggcct ctgtcccttc catcaggaga agagcccgtc gttctccatc
     2581 aacccggcct tgaacctgtt ctactgcttc ggttgccaaa agtccggtga tgccatcacc
     2641 ttcgtgcgcg aggtcgaaca cctcgacttc gtcgaggcgg tcgagcgact ggcgcaacgg
     2701 gcggggatca cgctccgcta cgacgaccgt cgcaccggtc aggaccggtc ccgcaagacc
     2761 cgcctcgccg aagcggtcgg cgccgcgatc gacttctacc accgccgcct caacgaatcc
     2821 cccgacggag gcgccgcccg caagtacctc cgcggccgcg gcttcgacgg cgacgcggcc
     2881 cgacgcttcc agctcggctg ggcccccgac gattgggacc ttctcagccg tcacctcgac
     2941 cagcgcggct tcgctcgcga cgacatcgaa ggcgccggcc tcgcgttcgt gaaccgggcc
     3001 aaccgcctcc aggaccagtt ccgctcccgg ctcatgttcc cgatctacga cgcccgcggt
     3061 gaacccgccg gattcggtgg ccgcaccctc ggcgatgacc aacccaaata caagaactcg
     3121 cccgagaccg cgatctacca caagagccgc ctcctctatg gcctcaactg ggccaaaggc
     3181 gagatcgtcg cccgcggcga cgtgatcatc tgcgaggggt acaccgacgt catgggcttc
     3241 gccctcgccg gcgcacccaa cgccgtcgca acctgcggaa ccgccctcgc cgacgaccac
     3301 ttccaagtcc tcaagaacct cgcccggcgc gtcaccctcg cctacgatgc cgatgccgcc
     3361 ggccagggcg ccgcggaacg ctggtaccga tgggaacagg aatacgagat ccagctccgc
     3421 gtcgccgacc tcccacccgg ccacgacccc ggagacctct ggcagcacga ccagccccgc
     3481 ctcctcctcg ccctcgagca cgccgaaccc ttcctgcagt tccgcctcga ccgggccatc
     3541 gccggcgccg acctcgcgac catcgaaggg cgggcccgcg ccgcgaccac cgccgccacg
     3601 atcatcgccg agcatcccaa cgagctcgtc cgcgaccagt acgtcatgaa gctcgccggg
     3661 gcgctcgaca tcgacgccga ccgtctccgc gacgccgtca cccaggcccg tcgcgcgcct
     3721 cgccgtcctt cacgcgaacc gccgtccgac accccgcccc ccgaaccccg gcgacgcccc
     3781 aacgtcgacc ggcgagagct cgacctgctc cgctgggcga tccacgaacc cgagctcatc
     3841 gccgactggc tcaacgagac gcttctccag gacccactcg cacgcgccgc ctaccatcgg
     3901 ctcgcgaaca gtccgtcgtt cgccgacgcg ctcgacggca ccgacgacga tgtacgcgca
     3961 ctcctcgagc gactcgccgt cgaagagccc gaagccgacg acgaacccga aacgctccgc
     4021 gctcggctac tggtcatcaa cgtcgaaccc gtcgcgaaac gcaccctcac gacgctcctc
     4081 gcggccggcg atgaggaacg agccggcgcg ctcaaacgca acctcgacga gctcgtccac
     4141 gcgcgcgaga tcggcgaatg ggagcgcgcc gaggaactcg ggcgacggac gctacagtgg
     4201 attgtctcga gcccggagta gccgccacga cgtgagccag cccaacaacg ctgctgcact
     4261 gacccccgtc gaccccgccg agctcgacca ggtcatcgag gagctcgtca tccgcgctcg
     4321 cgaacgggga tcggtcacct ccggcgagat cttcgcggcg ctccacaccc tcgaaccctc
     4381 aaccgaagag ctcgccgaga tctaccgccg catcgaagcc cgcggcatcg ccatccaaga
     4441 cgagatcacc gaagagctcg agctcgaaga cgcccgacgg cgcgacagcc ccgcaaccaa
     4501 ccaccgcggc ccccgaggcg aaccgacccg agccaccacc gccgtccgac ccggcgacgc
     4561 cgtcaccact cgaccagccc gcgtccgccg cgacctcgca tcggtcttcg acgagcccgc
     4621 ggcccgcagc gaccggggcg agactggcag cttcgacccg gtccgcatgt acctcaaaga
     4681 gatcggaaaa gtcccactcc tcaccgcacc gcaagaggtc aacctcgcca aacgcatcga
     4741 ggccggcgtc ctcgccgagg aacgcctcga atcccatcct gagatgaccg ccgggcagga
     4801 ggccagcgaa cacgccaacg tcgaagacgg cgagctcgcc aagcagcagc tcaccgaggc
     4861 caacctccgc ctcgtcgtct ccatcgccaa gcgctacgtc ggacgaggca tggccctcct
     4921 cgacctggtc caagaaggca acctcggcct aatgcgagcg gtcgaaaaat tcgactacac
     4981 gaagggcttc aagttctcca cctacgccac ctggtggatc cgccaagcaa tcacccgcgc
     5041 catcgccgac caggcccgca ccatccggat ccccgtccac atggtcgaga ccatgaacaa
     5101 ggtgctccgc cagcagcgcc agatgctcca agagctcgga cgagagccaa ccgtcgacga
     5161 agtcgcccgc aaggtcgacc tgacctccga caaagtgcgc gaaatccaac gcatctccca
     5221 agaaccgctc tcactcgaga cccccgtcgg agaagaagac gacagctccc tcggcgactt
     5281 cgtctccgac cccaccgccc ccgaacccac cgaagaagcc gagcaacagg gcctgaagga
     5341 agagatcacc cgagcgctcg aagagctcaa cgaccgcgaa cgagagatca tgcgactccg
     5401 cttcggcctc gacgacggcc aaccccacac cctcgaagag gtcggccgac gcttcaaggt
     5461 cacccgcgag cggatccgcc agatcgagtc caagaccctc gccaagctgc gccaccccac
     5521 ccgctcccaa cgactccggg cctacctcga cggcgcctga gcctgcggca tgatcgtgcg
     5581 gggccgtagc tcagtggtca gagcagagga ctcataatcc ttcggtcgca ggttcgatcc
     5641 ctgccggccc cacccaatgt ggcgagcgtg agcggccgac ggaagcgccc gcccatggga
     5701 agtcgggcga cgcagtgcga ggtgaacacc cgcggtgagc ctcggccagc aggtcgcgtg
     5761 tcagccaagg agcccaccaa gcgagccctg atcgcagcgc agaacgcgtc cgcgccgcaa
     5821 ctcgcgccaa aggagagtta gctgccgttc cacgccag
//
LOCUS       DATCCH010000337         2938 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_285486, whole genome shotgun sequence.
ACCESSION   DATCCH010000337 DATCCH010000000
VERSION     DATCCH010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285486"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..836)
                     /locus_tag="VIJ44_06410"
     CDS             complement(60..836)
                     /locus_tag="VIJ44_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010231249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY5165565.1"
                     /translation="MGDTVLYGRDGHVATITYNRPEVLNAINGALRQDLNAAWEQFRD
                     DEEAWVAIVTGAGRAFCVGADLRDGAASAGSWPGSFWEIPTINSFESGLEIWKPTIAA
                     VNGYCLGYGLTLVSACDFVIAADDAQFGMPEVRLGVPTIVGAIRLPQRIGMQPALELL
                     LTGDRIDAARAAEIGLAGRVVPRGELLNAARDLADRLCAGAPLAVRAVKEMAYRGQHL
                     PWIDAVRMGEAIRRIAASTEDAVEGRAAIAEGREPRWRAR"
     gene            complement(844..1317)
                     /locus_tag="VIJ44_06415"
     CDS             complement(844..1317)
                     /locus_tag="VIJ44_06415"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY5165566.1"
                     /translation="MTAEAELADRFAIHDLTMRYWMNVDRRDWPPVKDCFVPGTLVDY
                     SALMPVHAAVPAEEVVDRIAAAIEIYAVTVHNVGNHEVILDGDVARSEACIVAHHVYR
                     DRDRQGGRLPVAGLRYRDVMVRTGEGWRIRDRVATTDWRAWWESRPPTYVDGVHQ"
     gene            complement(1314..2267)
                     /locus_tag="VIJ44_06420"
     CDS             complement(1314..2267)
                     /locus_tag="VIJ44_06420"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY5165567.1"
                     /translation="MTHDTFSSTSIDLTAAPAALADVLIRRRVPIVAGYLTVTVAGAA
                     VVPLDRPAVVWWLVGLVALTCAGATHGMRRFLMDWLPLVVIAAGYDTVRAQAPDLLTR
                     AIVRPQLRFDEILFGGTAPTVQLQRLLDVQPGRVHWWDYLAWLVYLSHFVMTLAVAAF
                     LYVANRARFKRLATLILTVSVAGFITYFIIPAEPPWLASRQGALPHTTRVVHDIWSNL
                     GLPAVAKVFNGDAKLANPVAALPSLHAAWPFMLLLFLWPVLRRGRWLLIGYNALMVTV
                     LVYGAEHYVSDILLGWLYALVVFIAWSRYWARKDAATIQNP"
     gene            2344..>2938
                     /locus_tag="VIJ44_06425"
     CDS             2344..>2938
                     /locus_tag="VIJ44_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEY5165568.1"
                     /translation="MFVKVCGITTEDDALLAVALGADALGFVFASGSPRQVRIKEVGD
                     IVRRLPPGQATVGVFRDERRERVVEIVHSIGLTGAQLHGREPTSEVAWIAERVPFVIQ
                     AFRAGDPALATAADHAAHVVLLDAPEPGSGQVFDWSLAEGVPPGVRVILAGGLDADNV
                     GAAVRRVRPWGVDVSTGVETEPGSGRKDPIRLRRFVEA"
BASE COUNT          454 a         1056 c         1029 g          399 t
ORIGIN      
        1 ccgtctaccc gccaccgaca aactgagctc cactaccgaa gacttgtggg gacaccgggt
       61 tagcgtgctc gccagcgcgg ctctcgcccc tcagcgatcg cagcccgccc ttcgacggca
      121 tcctccgtgc tggcggcaat acgcctaatc gcctctccca tgcggaccgc gtcgatccag
      181 ggaaggtgct ggccgcggta ggccatctcc ttgaccgcgc ggacggcgag gggcgctcct
      241 gcacacaggc ggtcggccag gtcgcgggcg gcgttgagga gctcgccgcg gggcacgacc
      301 cggccggcga ggccgatctc ggcggcccgc gcggcgtcga tgcggtcgcc ggtgaggaga
      361 agctcgaggg cgggctgcat cccgatgcgc tgggggagcc ggatggcgcc gacgatggtg
      421 gggacaccga gacgcacttc gggcatgccg aactgggcgt cgtcggcggc gatgacgaag
      481 tcgcaggctg acacgagggt gaggccgtag ccgaggcagt agccgttcac cgcggcgatg
      541 gtcggcttcc agatctcgag accggactcg aacgagttga tggtggggat ctcccagaac
      601 gagcccggcc agctgcccgc cgatgcggcc ccgtcgcgca ggtccgcccc gacgcagaac
      661 gcccgtccgg cgccggtgac gatcgccacc cacgcctctt cgtcgtcacg gaactgttcc
      721 cacgccgcgt tgaggtcctg acgcaacgcc ccgttgatgg cgttgaggac ctcgggccgg
      781 ttgtaggtga tcgtggcgac gtgtccgtcg cgcccgtaca ggaccgtgtc acccatcgca
      841 gcgctactgg tggacgccgt cgacgtaggt cggcgggcgc gactcccacc aggcccgcca
      901 gtcggtggtc gcgacccggt cccggatccg ccagccctcg ccggtgcgga ccatcacgtc
      961 ccggtaccgg agccccgcga ccgggagccg ccccccttga cgatcccggt cccggtagac
     1021 gtgatgggcg acgatgcatg cctcgctgcg cgccacgtca ccgtcgagga tcacttcgtg
     1081 gttcccgacg ttgtggacgg tgacggcgta gatctcgatc gcagcggcga tccggtcgac
     1141 gacctcctcg gcgggcaccg cggcgtggac gggcatgagc gccgagtagt cgacgagcgt
     1201 ccccggcacg aaacagtcct tgaccggcgg ccagtcacgc cggtcgacgt tcatccagta
     1261 ccgcatggtg aggtcgtgga tcgcgaaccg gtccgccagc tccgcctcgg cggtcacgga
     1321 ttctggatcg tcgcggcgtc tttgcgggcc cagtaccgcg accacgcgat gaacaccacg
     1381 agcgcgtaca gccagccgag caggatgtcc gacacgtaat gctcggcgcc gtacacgaga
     1441 acggtgacca tcaacgcgtt gtacccgatc agcagccacc ggccccgccg gagcactggc
     1501 cagaggaaca gcagcagcat gaacggccac gccgcgtgca gcgatggcag cgccgcgacc
     1561 gggttggcga gcttggcgtc gccgttgaac accttcgcga cggcggggag accgaggttc
     1621 gaccagatgt cgtgcaccac tcgcgtcgtg tgcgggagcg caccctgacg gctggcgagc
     1681 cagggcggct ccgccgggat gatgaagtag gtgatgaacc cggcgacgct caccgtcagg
     1741 atcagcgtcg cgagccgctt gaaccgggcc cggttcgcga cgtacaagaa ggcagcgacg
     1801 gcgagcgtca tcacgaagtg cgacaggtac accaaccacg ccaggtagtc ccaccagtgc
     1861 acccgaccgg gctgcacgtc cagcagccgt tgcagctgca ccgtgggtgc ggtccccccg
     1921 aacaggatct cgtcgaagcg cagctggggg cgcacgatgg cccgcgtcaa caggtcgggc
     1981 gcttgggccc gcaccgtgtc atagccggcg gcgatcacca ccaacggcag ccagtccatc
     2041 aggaagcgcc gcatcccgtg cgtcgccccg gcgcacgtca acgccaccaa ccccaccaac
     2101 caccacacca ccgcgggccg gtcgagcggc acgaccgcgg cccccgcgac ggtgacggtg
     2161 aggtacccgg cgacgatcgg gacccgccgg cggatcagga cgtcagcgag cgcggcggga
     2221 gcagcagtga ggtcaatcga cgtcgaactg aacgtgtcgt gggtcatgcg gcgcccccag
     2281 gctcgccacg gctgggccag cgtagtgccc gccgtggacc ggaccctccg gtaccgtccg
     2341 ggggtgttcg tcaaggtctg cggcatcacc accgaggacg acgccctcct cgccgtggcg
     2401 ctcggcgccg acgccctcgg cttcgtgttc gcctccggca gcccccgcca ggtccggatc
     2461 aaagaggtcg gagacatcgt gcggcgcctc ccaccgggcc aggccaccgt cggggtgttc
     2521 cgagacgaac gccgggagcg ggtggtcgag atcgtccact cgatcgggct caccggcgcg
     2581 cagctgcacg gccgggagcc gacctcggag gtggcgtgga tcgccgagcg ggtcccgttc
     2641 gtcatccagg ctttccgggc cggcgaccct gccctggcca ccgcggcgga ccacgccgcc
     2701 cacgtcgtgc tcctcgacgc ccccgagccc ggctccggtc aggtgttcga ctggtccctc
     2761 gccgaggggg ttccgcccgg ggtgcgggtc atcctggccg ggggcctcga cgcggacaac
     2821 gtcggcgccg cggtgcgccg ggtccgcccc tggggggtcg acgtctccac cggcgtcgag
     2881 accgaacccg gctccggccg caaggacccc atccggctcc gccgcttcgt ggaggcgg
//
LOCUS       DATCCH010000338         2335 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_326528, whole genome shotgun sequence.
ACCESSION   DATCCH010000338 DATCCH010000000
VERSION     DATCCH010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326528"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /locus_tag="VIJ44_06430"
     CDS             complement(<1..867)
                     /locus_tag="VIJ44_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012894971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY5165569.1"
                     /translation="MDLLRFATAGSVDDGKSTLIGRLLYDSKAIFEDQLESVERTSRE
                     RGDEYTNLALLTDGLRAEREQGITIDVAYRYFATPKRKFIIADTPGHIQYTRNMVTGA
                     STADLAIILVDARNGLVEQSRRHTFLATLLRVPHLVLCVNKMDLVGFSEDVFEEIRAE
                     FTAFATKLNIPDLTFIPVSALNGDNIVTRSENTPWYDGPSLLHHLENVHVASDRNLVD
                     VRFPVQYVIRPMSSTHPDYRGYAGQVAGGVLKPGDEVVVLPSGFTSRIAAIDTADGPV
                     DEAYPPMSVTVRL"
     gene            complement(867..1787)
                     /gene="cysD"
                     /locus_tag="VIJ44_06435"
     CDS             complement(867..1787)
                     /gene="cysD"
                     /locus_tag="VIJ44_06435"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007071453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEY5165570.1"
                     /translation="MAVLRSDYLLSQLDVLEAESIHVMREVAAEFERPVLLFSGGKDS
                     IVMLALAERAFWPARIPFPVMHVDTGHNFSEVLEFRDRRVAEVGTRLIVASVQESIDA
                     GKVADETGRWASRNRLQTTTLLDAITEHRFDAVFGGARRDEEKARAKERMFSFRDEFG
                     QWDPKNQRPELWGLYNGRVHKGESIRIFPLSNWTELDIWQYVAHEDLELPSIYFAHHR
                     EVFVREGMLMAVTEFSQPGEGDVVFVEQVRYRTVGDATLTAAVRSDADTVHKIIEDVA
                     ATRITERGATRGDDKVSEAAMEDRKKEGYF"
     gene            complement(1887..>2335)
                     /locus_tag="VIJ44_06440"
     CDS             complement(1887..>2335)
                     /locus_tag="VIJ44_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020525808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEY5165571.1"
                     /translation="GGDVPRTGAVASPGPQYRDDDIAGDPGAGAAGGGLRLVVSRTRP
                     PGFVQRLADRTGAALLPLGSAGAKAAAVICGEADAYVHSGGQYEWDSAAPVAVARAAG
                     LHASRIDGSKLAYNQASPSVPDILICRAALADLLLGAISSVRRPDE"
BASE COUNT          367 a          776 c          820 g          372 t
ORIGIN      
        1 gagccgtacc gtcaccgaca tgggcgggta ggcctcgtcg accggaccgt cggccgtgtc
       61 gatcgcggcg atccgggagg tgaagccgga cggcagcacg acgacctcgt cgcccggctt
      121 caggacaccg ccggcgacct ggcccgcata accgcggtag tcggggtggg tcgaggacat
      181 gggcctgatc acgtactgca ccgggaaccg gacgtcgacg aggttgcggt cggacgccac
      241 gtggacgttc tccaggtggt gcagcaacga ggggccgtcg taccacgggg tgttctcgct
      301 gcgggtgacg atgttgtcgc cgttgagggc ggacaccggg atgaaagtca ggtcagggat
      361 gttcagcttc gtcgcgaatg cggtgaactc ggcccggatc tcctcgaaga cgtcctcgga
      421 gaacccgacg aggtccatct tgttgacgca gagcacgagg tgcggcaccc gcagcagcgt
      481 cgcgaggaag gtgtgccggc ggctctgctc gacgagcccg ttgcgggcgt cgaccaggat
      541 gatcgcgagg tcggccgtcg acgcacccgt gaccatgttc cgggtgtact ggatgtggcc
      601 cggggtgtcg gcgatgatga acttgcgctt gggcgtcgcg aagtatcggt aggccacgtc
      661 gatggtgatg ccctgctcgc gctcggcacg caggccgtcg gtgagcagcg ccaggttggt
      721 gtactcgtcg ccccgctcgc ggctggtgcg ttccaccgac tcgagctggt cctcgaagat
      781 ggccttgctg tcgtagagca ggcgaccgat gagcgtgctc ttgccgtcgt cgaccgaacc
      841 cgcggtagcg aatcggagca ggtccatcag aagtagcctt ccttcttgcg gtcttccatc
      901 gcggcctcgc tgaccttgtc gtcgccgcgc gtggccccac gctcggtgat ccgggtggct
      961 gcgacgtcct cgatgatctt gtgcacggtg tcggcgtcgg agcggacggc cgccgtgagc
     1021 gtcgcgtcgc cgaccgtgcg gtagcgcacc tgctcgacga agacgacgtc gccctcgccc
     1081 ggctgggaga actcggtgac cgccatcagc atgccctcgc ggacgaacac ctcgcggtgg
     1141 tgcgcgaagt agatggaggg cagctcgagg tcctcgtggg cgacgtactg ccagatgtcc
     1201 agctcggtcc agttcgagag cgggaagatc cggatgctct cgcccttgtg gacgcggccg
     1261 ttgtacagcc cccagagctc ggggcgctgg ttcttcgggt cccactggcc gaactcgtcg
     1321 cggaaggaga acattcgttc cttggccctg gccttctcct cgtcccgccg cgcgccgccg
     1381 aacaccgcat cgaaccggtg ctcggtgatc gcatcgagga gcgtcgtcgt ctgcagccgg
     1441 ttgcggctcg cccagcgccc ggtctcgtcg gcgaccttgc cggcgtcgat ggactcctgc
     1501 accgacgcga cgatcagcct ggtgcccacc tcggctaccc ggcgatcccg gaactcaagg
     1561 acctcggaaa agttgtgacc ggtgtccacg tgcatgacgg ggaacgggat ccgggccggc
     1621 cagaacgccc gctcggccag ggcaagcatc acgatcgagt ccttgccgcc ggagaacaac
     1681 agcaccggcc gctcgaattc ggcggcgacc tcgcgcatca cgtgaatgga ctcggcctca
     1741 aggacgtcga gctgggagag caggtagtcg gaacgcagca ctgccatcag cggaactcca
     1801 ggtcgtggta cgcgggcgga tcggaccaaa cgggcggcca tgctgagcca ggggccggcc
     1861 agccgtcggc cgagaccggt gcgggcttac tcatcggggc gccgaacgga gctgatcgcc
     1921 ccgagcagca agtccgcaag cgcggctcga cagatcagga tatccggtac cgagggactg
     1981 gcctggttgt aggcgagctt cgagccgtca atccgggacg catgcagccc ggccgcgcgg
     2041 gcgaccgcga caggtgcggc cgaatcccac tcgtactggc cgccgctatg cacatacgcg
     2101 tcggcctccc cgcagatcac ggcggccgct ttcgccccgg ccgaaccgag tggcagcagc
     2161 gcggccccgg tccgatcggc gagccgctgc acgaacccgg gcggcctggt gcggctgacc
     2221 accagtctga gcccgccccc ggccgcgcca gcgccgggat ccccggcaat gtcatcgtct
     2281 cggtactgcg ggccgggact ggccacggca ccagttcgcg ggacatcacc gcccg
//
LOCUS       DATCCH010000339         2382 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_203230, whole genome shotgun sequence.
ACCESSION   DATCCH010000339 DATCCH010000000
VERSION     DATCCH010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203230"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1085)
                     /locus_tag="VIJ44_06445"
     CDS             complement(<1..1085)
                     /locus_tag="VIJ44_06445"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEY5165572.1"
                     /translation="MPIVLGVDCATDATTVELRDADDGKVFSSGRASHPAAAQATTQD
                     PSVWWQGLVDARHEAGGALGVGAVSVAAPIQGLVLLDPDHKPLGSAILADDPRGSAEI
                     GELLRALGNAADWTVAVGSVPDASFPIAKLAHLRVAEPRRFGAVTKVLAPHDWLTYRL
                     SRRFVTDRGDASSTGYWSPRENAWRIDLLTLMDDAKDWGACLPRVAGPSDAAGDREGV
                     VIAAGTGSPMSAALGIGLEPRDLLVVLDDQITVSAVRERPTEDPTGVVTGFADATGRF
                     LPQVRLRNGMNVIDAVVRFLGVDRHRFDQLALSAPPGSAGVALLPGFEPERASRQPDV
                     GGVLTGLRADVGPEHLARAAVEGVVCSV"
     gene            1138..1872
                     /locus_tag="VIJ44_06450"
     CDS             1138..1872
                     /locus_tag="VIJ44_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165573.1"
                     /translation="MSTLPPSPPPPAAWQPPTIGAAARVRAAYAQRQQTDYYFTGPGL
                     NVFLTIITCGIFGFFLFYQLMRRDRDHNRRRLDLLDGANAFAWEQATERGLADELRPA
                     FERIAAQLEVLRRLTTEFRDPTIWLVIDIVASGARFFGASIAQMVGFILIDKDLDTHD
                     RAEGAIEADLSAIYARLGVTLPSPDPARVKGQQNYVARVIVSILTCSIYVFWWIYNMQ
                     VDGNRHLEMNWPYDNSIVEAVETLGG"
     gene            complement(1876..2118)
                     /gene="xseB"
                     /locus_tag="VIJ44_06455"
     CDS             complement(1876..2118)
                     /gene="xseB"
                     /locus_tag="VIJ44_06455"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEY5165574.1"
                     /translation="MNPKASEPGYVEAMAELEAILDELEGDQLDVDLLAERVQRASEL
                     LKVCRARIARAQQDVDRIVSDLDDFDTSEGDLAPEG"
     gene            complement(2115..>2382)
                     /locus_tag="VIJ44_06460"
     CDS             complement(2115..>2382)
                     /locus_tag="VIJ44_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008168009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEY5165575.1"
                     /translation="RSAARAAAGREERIVAGVRRSMRAERVRIEAADARLRALDPRRV
                     LERGYSITRDAHGRAVRAAADAAVGSLLVTETADGAIRSWVEEQ"
BASE COUNT          385 a          858 c          789 g          350 t
ORIGIN      
        1 accgagcaga caaccccttc gaccgcggcg cgagcgaggt gctcgggtcc gacgtcagcg
       61 cgcagtccgg tgagaacccc tccgacatcg ggctggcggg aggcccgttc gggctcgaac
      121 cctgggagga gcgccacccc ggccgatcct ggaggggcac tgagcgccag ctggtcgaac
      181 cggtgccggt cgacgcccag gaaacgcacc accgcatcga tcacgttcat gccgttgcgg
      241 agccgcacct gcggcaagaa gcgcccggtg gcgtcggcaa agcccgtgac gacacccgtc
      301 gggtcctccg tcggtcgttc ccggaccgcg ctgaccgtga tctggtcatc gaggacgacc
      361 agcagatctc ggggttcgag cccgattccc aacgccgcgc tcatgggcga tccggtcccg
      421 gcggcgatga cgacgccctc gcgatctccc gccgcgtcgc tgggcccggc gacccgcggg
      481 aggcaggctc cccagtcctt ggcgtcgtcc atgagcgtca gcaggtcgat ccgccaggcg
      541 ttctctcgcg gggaccaata gccggtgctg gaggcgtcgc cgcggtcggt gacgaagcgg
      601 cgactcaagc ggtacgtcag ccagtcgtgg ggagcgagca ccttggtgac cgcgccgaag
      661 cgtcgcggct cggcaaccct caagtgagcg agcttcgcga tgggaaacga cgcgtcgggc
      721 acggagccca ccgccaccgt ccagtcggcg gcgttgccca gcgcgcggag cagctccccg
      781 atctcggcgg atcccctcgg gtcgtcggcg aggattgccg aaccaagcgg cttgtggtcg
      841 gggtcgagca ggaccaagcc ctggattggg gcagccacgg acaccgcgcc caccccgagc
      901 gcgccaccag cctcatgccg ggcgtcgacc aggccctgcc accacacgct cgggtcctgg
      961 gtggtcgctt gggccgcggc gggatgcgaa gcccgtcccg acgagaacac cttgccgtcg
     1021 tccgcgtcgc gcagctcgac cgtggtcgcg tcggtcgcgc agtcgacacc aagcacaatt
     1081 ggcacgagcc gcctcccacc gaacgcccga ttggggcaaa acgatagggt ccggcgcatg
     1141 agcacgctac cgccgtcgcc acccccgccg gctgcgtggc agcccccgac catcggtgcg
     1201 gccgctcgcg tgcgtgccgc ctatgcgcaa cgtcagcaga ccgactacta cttcaccggt
     1261 cccggactga acgtgttctt gacgatcatc acctgcggca tcttcgggtt cttcctcttc
     1321 taccagctga tgcgacggga ccgcgaccac aaccgacggc gcctggatct cctcgacggt
     1381 gccaacgcgt tcgcctggga gcaggcaacc gagcggggcc tcgccgacga gctgcggccc
     1441 gcattcgagc gaatcgcggc gcaactcgag gtgcttcgac ggctgaccac cgagttccgc
     1501 gacccgacca tctggctggt gatcgacatc gtggccagcg gcgcccgatt cttcggagcc
     1561 agcatcgccc agatggtcgg gttcatcttg atcgacaagg acctcgacac tcacgaccgc
     1621 gccgagggcg ccatcgaagc cgatctgagc gcaatctacg cccgactcgg cgtgacgttg
     1681 ccgagccccg atccggcccg ggtgaaagga cagcagaact acgtcgcacg cgtcatcgtg
     1741 tcgatcctga cgtgcagcat ctacgtgttc tggtggatct acaacatgca ggtcgacggc
     1801 aaccgacacc tcgagatgaa ctggccctac gacaattcca tcgtcgaagc ggtcgagacc
     1861 ctcggcggct gagactcaac cctccggggc gaggtccccc tcgctggtat cgaagtcgtc
     1921 gaggtccgac acgatgcggt cgacatcctg ctgcgctcgc gcgatgcggg cccggcagac
     1981 cttcaacagc tcactcgccc gttggacccg ctcggcgagc agatcgacgt cgagctggtc
     2041 gccttcgagc tcgtcgagaa tcgcctccag ctcggccatc gcctccacgt acccgggttc
     2101 ggaggccttg gggttcattg ctcctccacc caactgcgaa ttgctccgtc ggcagtctcg
     2161 gtgacgagca gcgacccgac ggccgcgtca gccgcggcgc gcaccgcccg gccgtgcgcg
     2221 tcgcgggtga tgctgtagcc acgctcgagg acccggcggg ggtcgagagc gcgcagacgg
     2281 gcgtcagccg cctcgatgcg gacgcgctcg gccctcatcg atcggcgaac gccagcgacg
     2341 atgcgttcct cgcggccggc ggcggcgcgg gccgcggagc ga
//
LOCUS       DATCCH010000340         2476 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_142582, whole genome shotgun sequence.
ACCESSION   DATCCH010000340 DATCCH010000000
VERSION     DATCCH010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142582"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..383)
                     /locus_tag="VIJ44_06465"
     CDS             complement(33..383)
                     /locus_tag="VIJ44_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165576.1"
                     /translation="MAERPYDVPQRIVIPNLTGMIVPAALRSADDVGFTLASADPMMR
                     LADVASSGRWQVAEQEPVGGATRFRGDTVAVTFRRDHRGPDAGDREPRNPLPAVRVES
                     APLPQADALDADVI"
     gene            complement(516..2324)
                     /locus_tag="VIJ44_06470"
     CDS             complement(516..2324)
                     /locus_tag="VIJ44_06470"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEY5165577.1"
                     /translation="MTTDHLATMIRDNVQRLASRPAMRYQDGAGWRSVSYTELGEKIR
                     STANALVESGVQPGDMVGIFSPNRPEWTIADFAILSAGAVSVPIYPTNTAKQAEYIVA
                     DADLSVIFVGDQAQYDKVVAFRSRTPGLKRIVVFDEKATLAGEDCRTFADFLATGAAS
                     SRDEEVDARIAAGSPDDLATLIYTSGTTGDPKGAVLTHANFFHQVRALDDRFDIGPGD
                     TSLCFLPLSHAYERAWSIYVFAKGAENCYVLDPSRVVQAMAEVRPSTMVSIPRLYEKI
                     HATVLDRVERGSAPRAKLFGWSIGVGGRYQRRRTAGESINPLLRVEHAVADRLVLAKV
                     RDVVGGEKKVFAAGGAPLSKDIEEFFFATGILICQGYGLTETAALVTCNYPGGFKFGT
                     VGKPVLGTDLRIAADGEIQVRGGNIMSGYYGRPDDTAAAFEDGWFKTGDQGMVDADGF
                     VVITDRLKDIIITSQGKNIAPQHVESALGSDPYIEQLIVIGDRRAYLSALIEPDFAML
                     ERYADEHGIPHPSRADLVVQPQIRALFDSRIAELSRELANYERVKRYTLVPREFTREN
                     GELTPTLKLKRRVIEQEYADVIEAMYGATRQAPAAR"
BASE COUNT          375 a          829 c          842 g          430 t
ORIGIN      
        1 gacctaccgg ctcctgagat cggccggcgg ggtcagatca catccgcgtc gagcgcgtca
       61 gcctgcggca gtggcgcgga ttcgactcga accgccggca gcggattcct gggctcgcga
      121 tcaccggcgt cgggtccccg gtgatcccgc cggaacgtga cggcgaccgt gtcgccgcgg
      181 aaccgggtcg cgcccccgac cggttcctgc tcggcgacct gccagcggcc ggaggatgcc
      241 acgtcggcca gtcgcatcat cggatcggcc gaggcgagcg tgaaacccac gtcgtcggcg
      301 gatcgcagtg ccgccgggac gatcatcccc gtcaggttcg ggatgacgat gcgctgcgga
      361 acgtcatagg gtcgttcggc caaccgccct gcacgcatga cgaccctcca gacggtgccg
      421 gtgtgcgagc caccaacgta ctgctcccgg gggcagacgg cccggatgcc cttctcccgt
      481 cagtgaacgg atccgacgcc gatcggacgt tcgagctatc gcgcggccgg ggcctgccgg
      541 gttgccccgt acatcgcctc gatgacgtcc gcgtactcct gctcgatcac ccgccgtttg
      601 agcttgagcg tgggtgtcag ctcgccgttt tcccgggtga actccctggg caccagggtg
      661 tatcgcttca ctcgctcgta gttggccaat tcgcgggaga gttccgcgat gcggctgtcg
      721 aacagggccc ggatctgcgg ttgcacgacg agatccgcgc gcgacgggtg cgggatcccg
      781 tgctcgtcgg cgtaccgctc cagcatagca aagtccggtt cgatcagcgc ggagaggtac
      841 gcccgccgat caccgatgac gatgagctgc tcgatgtacg ggtcgctccc gagtgccgat
      901 tcgacgtgtt gcggggcaat gttcttgccc tgcgacgtga tgatgatgtc cttgagtcgg
      961 tcggtgatca cgacgaatcc gtcggcatcg accatcccct ggtctccggt cttgaaccag
     1021 ccgtcctcga acgcggccgc ggtgtcgtcg ggtcggccgt agtagccgct catgatattc
     1081 ccgccacgga cctggatctc gccgtcggcg gcgatgcgga ggtcggtgcc cagcaccggt
     1141 ttcccgacgg tgccgaactt gaagccgccg gggtagttgc acgtgacgag ggcggccgtc
     1201 tcggtgagcc cgtagccctg gcagatcaag atcccggtgg cgaagaagaa ctcctcgatg
     1261 tctttggaca ggggggcgcc tcccgcggcg aagaccttct tctccccgcc cacgacgtca
     1321 cgaaccttgg ccagcacgag ccggtcggcc actgcgtgct cgacccggag cagcgggttg
     1381 atcgattccc cggcggtgcg ccgtcgctgg tagcgcccgc ccaccccgat cgaccaaccg
     1441 aagagcttgg cccgcggcgc cgatccccgc tcgacccggt ccagcacggt cgcatggatc
     1501 ttctcgtaga ggcgggggat gctgaccatc gtcgagggac ggacctcggc catcgcctgc
     1561 accacccggc tcgggtccag gacataacaa ttctccgcgc ccttggcgaa gacatagatc
     1621 gaccaggccc gttcgtaggc gtggctcaac ggtaggaagc acaggctggt gtcgccggga
     1681 ccaatgtcga agcggtcgtc cagcgcccgg acctgatgga agaagttggc gtgggtgagc
     1741 accgcgccct tcggatcgcc ggtcgtgcct gacgtgtaga tcagcgtcgc gaggtcgtcg
     1801 ggtgaacccg cggcgatccg ggcgtcgacc tcctcgtcgc ggctcgacgc cgcacccgtg
     1861 gccaggaagt cggcgaaggt gcggcaatcc tcgccggcga gcgtggcctt ctcgtcgaag
     1921 acgacaatcc gcttcagccc gggtgtacgg gatcggaagg cgacgacctt gtcgtactgc
     1981 gcctggtcgc ccacgaagat caccgataga tccgcgtcgg cgacgatgta ctcggcctgt
     2041 ttggcggtgt tggtggggta gatcgggacg gacaccgccc ccgcgctcag gatggcaaag
     2101 tcggcgatgg tccactccgg ccggttgggt gagaagatcc ccaccatgtc gccgggctgg
     2161 acgccggact cgacgagagc gttggccgtc gaacggatct tctcgcccag ttcggtgtag
     2221 gagacagacc gccagccggc cccgtcctga taacgcatgg ccggccggga cgccaaccgc
     2281 tgcacgttgt cgcggatcat ggtcgcgagg tggtcggtcg tcacgggctc tccccaattc
     2341 ggcccgacca cgccgtcggg ttcttcgctg ggcgcactga cgcgccgagg agagtcttcc
     2401 atctccggcc cttcgggggc cctcccggtg ttgatcatgg ggcgcggccc gctccgatcg
     2461 gtgtgtcgtc ggcgtt
//
LOCUS       DATCCH010000341         2278 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_535598, whole genome shotgun sequence.
ACCESSION   DATCCH010000341 DATCCH010000000
VERSION     DATCCH010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535598"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VIJ44_06475"
     CDS             <1..256
                     /locus_tag="VIJ44_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003987348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEY5165578.1"
                     /translation="AAGRFTVCAQTETAPYKYVSVEGAVVAVDDPVEPAERRAIAHRY
                     LGPELGDLYLEATAADAPTTVTFRMRPERWLSVDYTKQFG"
     gene            complement(301..1590)
                     /locus_tag="VIJ44_06480"
     CDS             complement(301..1590)
                     /locus_tag="VIJ44_06480"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEY5165579.1"
                     /translation="MSPAAVRDGMIPKERYTSSAFSDLEFERLWPRVWQVACREEQVA
                     AVGDYCEYSIGEESVLVVRSDAETIRAFFNACLHRGTRLVDGCGQVGETFVCPFHAWR
                     YALDGRLVEVVDAHEFGTLPEGLGLREVRCERWGGFVFVNLDPDAEPLGEFLDPLPEL
                     LGRYRLDEMRLRSYQTTVLPANWKVVVDAFNEAYHVQGTHPQLLPWTDDVSIEYEQLG
                     KHAHYGRLPSARRRLRPSPRLGIADADVDEGAILSGLVAGLGGAFLAEERALVDEVRA
                     APLPPGANLLEVYQARRMELLRDRGFDVSGLEAEQMTSADDVYVFPNLVGPIYPGSAI
                     VFRVRPNGVDVDSALKDTWVLEWPRSDREWRMPEQRWYPDWRDKDWGPITTQDYENMA
                     RVQAGMKSRGFEGLRLNPRQESNLVHMHQVIDEYLDA"
     gene            1619..>2278
                     /locus_tag="VIJ44_06485"
     CDS             1619..>2278
                     /locus_tag="VIJ44_06485"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY5165580.1"
                     /translation="MEFNLADLFECVADHVSDRVALTCGDRHLRYETLDDRANRLAHG
                     LAGLGVETGDHVACYLRTGVEYVETMLACFKLRAVPINVNDRYVTDEVVDVCADADAV
                     ALVADPQLLADVAAVAVRLPALRTTLIVGSAYDDLIDRGSPGRDFGPRSADDHYILYT
                     GGTTGRPKGVVWRHEDIFFAALGGGNAGGPPIERPDAIGPTAVTNRAQRLAPFLPPGD
                     PG"
BASE COUNT          364 a          870 c          723 g          321 t
ORIGIN      
        1 cgccgcgggg cggttcaccg tctgcgcgca gaccgagacg gcgccataca agtacgtcag
       61 cgtcgaaggc gcagtcgtcg ccgtcgacga tcccgtcgaa cctgccgagc ggcgagcaat
      121 tgctcaccgg tacctcggcc ccgagctcgg cgacctgtat ctcgaagcca ccgcggccga
      181 cgcccccacg acggtcacgt tccggatgcg ccccgagcgc tggctgagcg tcgactacac
      241 caagcagttc ggataatcgt cggccctccg cgtcgagggc gcagctgccc gtcgggacga
      301 tcaggcatcg aggtactcgt cgatcacttg atgcatgtgc accaaattcg attcctggcg
      361 cgggttgagc cgcaacccct cgaagccccg ggacttcata ccggcctgga cccgggccat
      421 gttctcgtag tcctgagtgg tgatcggacc ccaatccttg tcgcgccagt ccgggtacca
      481 ccgctgctcc ggcattcgcc actcccgatc ggagcgaggc cactccagca cccaggtgtc
      541 cttgagcgcc gagtcgacat cgacgccgtt cggacggacc cgaaacacga tcgcgctccc
      601 cggatagatc ggccccacca ggttcgggaa cacgtacacg tcgtcggcgc tggtcatctg
      661 ctccgcctcg aggccgctga cgtcgaaccc gcggtctcgg agcagctcca tgcggcgggc
      721 ctggtagacc tccaacaggt tcgcgcccgg cgggagcggc gcggcgcgca cctcgtcgac
      781 cagggcgcgc tcctcggcca ggaacgcgcc cccgagaccg gcgacgagtc ccgacaggat
      841 cgcgccctca tcgacgtccg cgtcggcgat cccgagccgg ggactcggac gcagccgccg
      901 ccgcgcgctc ggtagccgcc cgtaatgcgc gtgcttcccg agctgctcgt actcgatgct
      961 cacatcgtcg gtccacggca gcagctgcgg atgggtgccc tgcacgtggt acgcctcatt
     1021 gaacgcatcg acgacgacct tccagttcgc gggaagcacc gtggtctgat acgagcggag
     1081 ccgcatctcg tcgaggcggt agcggcccaa cagctcgggg agtggatcaa ggaactcgcc
     1141 gagcggctcg gcgtccgggt ccaggttcac gaacacgaac ccaccccacc gctcgcaccg
     1201 cacctcgcgc aaccccagtc cctcaggcag ggtcccgaac tcgtgcgcgt cgacaacctc
     1261 gaccaggcgc ccgtcgagcg cgtaccgcca ggcgtggaac gggcagacga acgtctcgcc
     1321 gacctgcccg cacccgtcga cgagccgggt gccccggtgc agacaggcgt tgaagaaagc
     1381 gcggatcgtc tcggcgtcgg agcgcaccac caggaccgac tcctcgccga tcgagtactc
     1441 gcagtagtcg ccgacggcgg cgacctgctc ctcgcggcac gccacctgcc agacccgagg
     1501 ccagagccgc tcgaactcga gatccgagaa cgccgacgac gtgtaccgct ccttcgggat
     1561 catgccgtcg cgcaccgctg ccgggctcac ggacctgacg gtagcgtcgc cccggccgat
     1621 ggagttcaac ctcgctgacc tgttcgagtg cgtggccgac cacgtctcgg accgcgtggc
     1681 gctgacctgc ggggaccgcc acctccgcta cgagacgctc gacgaccggg cgaaccgcct
     1741 cgcccacggc ctcgccggcc tcggcgtcga aaccggggac cacgtggcct gctacctgcg
     1801 caccggcgtc gagtacgtcg agacgatgct ggcctgcttc aagctgcggg cggtcccgat
     1861 caacgtcaac gaccggtacg tcaccgacga agtcgtcgac gtctgcgccg acgccgacgc
     1921 ggtggccctc gtcgccgatc cccagcttct ggccgacgtc gccgccgtcg ccgtccgact
     1981 cccggcgctt cgcaccacgc tcatcgtcgg ctcggcctac gacgacctga tcgaccgcgg
     2041 atccccgggg cgggacttcg ggccccgctc cgcggacgat cactacatcc tctacaccgg
     2101 gggcaccacc gggcgaccca agggtgtggt gtggcgccac gaggacatct tcttcgccgc
     2161 gctcggcggc gggaacgccg gcggcccacc gatcgaacga cccgacgcga tcggcccgac
     2221 cgcggtcacg aaccgggcgc agcggctcgc cccgttcctc cccccgggcg accccggc
//
LOCUS       DATCCH010000342         3892 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_392546, whole genome shotgun sequence.
ACCESSION   DATCCH010000342 DATCCH010000000
VERSION     DATCCH010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392546"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="VIJ44_06490"
     CDS             <1..770
                     /locus_tag="VIJ44_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011028961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEY5165581.1"
                     /translation="IGILGSTFDGSYEPIAEIAGALDDLHTGGGPDVLIHVDAASGGF
                     VAPFLDADLEWDFRLPRVASINASGHKYGLVYPGVGWVVWRDMAALPEELVFSVSYLG
                     GSEDTFSLNFSRPGAPVVAQYYNFLRLGHDGYRRVQATTRDIARHLAEGIDELGPFEL
                     ITRAEHLPVFAFTLKKEEQRYSAFDVSRCLREGGWLVPAYTFPPHLENLAVLRIVVRN
                     AFSTDLADLLLDALKTAIPQLERQAGPLSRPIPSFHH"
     gene            1382..2308
                     /locus_tag="VIJ44_06495"
     CDS             1382..2308
                     /locus_tag="VIJ44_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020658834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEY5165582.1"
                     /translation="MSPDVWSATFPALGTTATLVVTQHAALPRARAILEDVLADVDRS
                     CSRFRADSDLTRVNDGGGQPVAVGTTLIDAIEVALRAARLTDGDVDPTIGSALRLLGY
                     DGDFATVAATGGPLSYRVERVPGWWTVDVDHAAGTVRVPDGVQLDLGATAKALAADRA
                     AIRIADDTACGTLVSLGGDVAAAGSGPEAGWAVLVTDDHTAGPDAPGETVAIRRGGMA
                     TSSTAVRRWRRGDDDLHHIVDPTTGQAASTIWRTVTVVAASCVDANIATTATVVRGEA
                     AIDWLAALNLAARLVRPDGTVLRIGDWPEPIR"
     gene            2305..3402
                     /locus_tag="VIJ44_06500"
     CDS             2305..3402
                     /locus_tag="VIJ44_06500"
                     /inference="COORDINATES: protein motif:HMM:NF013918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEY5165583.1"
                     /translation="MSPVIATTGPTFLWYLARGSGIAALIILTLGMVLGLVTSVRWSN
                     PRWPRFVVELLHRNASLIAFSLVVIHVATVIIDGFAPIGWTDAVIPFLSPYRPVWLGF
                     GAIAFDLLVAILATSLLRHRIGHRTWRWVHWFAYLCWPLVVVHGLATGSDAKVGFVLI
                     LTVVCIAAVIFAIWWRLAVDWREHTGVRVGALVASFVAPLVLVAWLTTGPLAAGWARH
                     AGTPANVLAKIGTTPTAPSTPAPTATASVPTAPFSASLTGSLTRSNPNASGQVTLRID
                     TTLSGGALGAVTVDLTGQPVNGGGVILASSQVGFGPIGAPAVYRGSVTTLRGQRIIAT
                     MRASGRPTIKLTINVQVASSGSRVTGTVLAQ"
     gene            complement(3515..>3892)
                     /locus_tag="VIJ44_06505"
     CDS             complement(3515..>3892)
                     /locus_tag="VIJ44_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY5165584.1"
                     /translation="CRELTDAYRDAGGTGPCILIRRAWLGEPPRNQVEQQLDRYRSYT
                     PRGAQVHWGGDELAGGADASTVAGQLGDAVSVAGADALNVRVHVPGVTFDAARAQIAG
                     LGDEVVPMVRTALSGTQPGGAQP"
BASE COUNT          529 a         1484 c         1266 g          613 t
ORIGIN      
        1 tcatcggcat cctcggctcg accttcgacg gcagctacga gccgatcgct gagatcgccg
       61 gcgcgctcga tgacctccac accggcggcg gacccgacgt gctgattcac gtcgacgcgg
      121 cgtcgggggg cttcgtggcc ccgttcctcg acgcggacct cgaatgggac ttccggcttc
      181 cgcgggtcgc gtcgatcaac gcctccggcc acaagtacgg gctcgtgtac ccgggcgtcg
      241 gctgggtcgt ctggcgtgac atggcggcgc tgcccgagga gctggtgttc tcggtcagtt
      301 atctcggcgg cagcgaagac acgttctcgc tcaacttctc gcgtccgggc gcacccgtcg
      361 tagcgcagta ctacaacttc ctccgactcg gccacgacgg ctaccggcgg gtgcaagcga
      421 ccacccgtga catcgctcga caccttgccg agggaatcga cgaactcggc ccgttcgagc
      481 tgatcacccg ggccgagcac cttccggtct tcgccttcac gctcaagaaa gaggagcagc
      541 ggtactcggc cttcgacgtt tcccgatgct tgcgggaggg cggctggctg gtgcccgcct
      601 acaccttccc ccctcacctg gagaacctcg cggtcctgcg aatcgtcgtc cgcaacgcgt
      661 tcagcacgga cctcgccgac ctcctgctcg acgccctcaa gaccgcgatc ccacagctgg
      721 aacgccaggc cggccccctg agccgcccca tcccgtcatt ccaccactag gccccagccc
      781 gcttcccctc cgccgcgcca gtccaccggg cgcccggacc gggtgggggc ttcgcctgac
      841 ctccccgggc caggggggat cgcggccacc ggcgccgtgg cggcgcggat ccgcatgtgg
      901 gggttcgatc gccgcccacg gaaccatttt ccgggtttgg tcgttttctt gttgtctcct
      961 taccagggac tgcgaacctg gctgtggcgg cccgccgtcc tccgatgccg gcgggtctgg
     1021 aaccctggtg atgtcatcac ccgacctgtc ggcgcgacaa gaaagggtcc gcgatgagac
     1081 ttggagcagc cgaccgggac tccgggctcc ggcgagtctc ccggatgacg cgctggctcg
     1141 ttgccggctc ggtgacggtc gccggggtcc tctccgccgt cgtcgcgcaa gccctccctg
     1201 gcaactccgg ttccgtggcc acgccgagcc ccggcgcggg cacccccagc cccgcggcgt
     1261 ccccaaggac gacgacgacc agtccgaccg tcaacagccc ctccgtcacc gaccccccga
     1321 tccagcctgc accgccaccg gttcccactc aacgtcgcgc ggtcactcaa tccggaggct
     1381 cgtgagccca gacgtctggt cagcgacgtt cccggcgctg ggcacgaccg caaccctcgt
     1441 cgtcacccag catgcggcac tcccccgcgc ccgcgccatc ctcgaggatg tcctcgccga
     1501 cgtcgaccgg tcctgcagcc ggttccgcgc cgactctgat ctgacccgcg tgaacgacgg
     1561 tggtggtcaa cccgtggcgg tcggcaccac gctgatcgac gcgatcgagg tcgccctgcg
     1621 cgccgctcgg ctcaccgacg gggacgtcga cccgacgatc gggagtgcgc tgcggctcct
     1681 cggctacgac ggcgatttcg cgactgtcgc cgccaccggc ggaccgttgt cctaccgcgt
     1741 cgagcgggtg ccgggatggt ggaccgtcga cgtcgatcac gccgcgggca cggtccgagt
     1801 tcccgacggc gtccaactcg acctcggtgc caccgccaag gccctcgccg ccgatcgagc
     1861 cgcgatcagg atcgccgacg acacggcgtg cggcacgctc gtcagtctcg gaggcgatgt
     1921 cgccgcagcc gggtcgggcc ccgaggccgg atgggcggtg ctcgtgaccg acgaccatac
     1981 cgccgggccg gacgcgcccg gggagacggt cgcgatccga aggggtggca tggccacctc
     2041 cagcaccgcg gttcgccggt ggcgccgcgg cgacgacgac ctccatcaca ttgtcgatcc
     2101 cacaaccggc caagccgcga gcacgatctg gcggacggtg accgtcgtcg ccgccagctg
     2161 cgtcgacgcg aacatcgcga cgactgccac ggtggtgcgc ggcgaagccg ccatcgactg
     2221 gctggctgcg cttaaccttg ccgctcggtt ggtacgcccg gacggcaccg tcctgcggat
     2281 cggtgattgg cccgagccga tccgatgagc cccgtcattg ccaccaccgg accgacgttt
     2341 ctctggtacc tggcgcgtgg cagcggcatc gccgcgctga tcatcttgac cctcggcatg
     2401 gtgctcggtc tcgtcacgtc ggtgcgttgg agcaatccgc gctggccacg cttcgtcgtc
     2461 gagctcctgc accgcaacgc gtcgctcatc gccttcagcc tcgtcgtcat ccacgtcgcc
     2521 accgtcatca tcgacggctt cgctccgatc gggtggacgg acgcggtgat cccgttcctc
     2581 tcgccgtacc ggccggtgtg gttggggttc ggcgccatcg cgttcgacct gctcgtcgcc
     2641 atcctcgcca ccagcctctt gcgacaccgg atcggacacc gaacctggcg gtgggtgcac
     2701 tggttcgcgt acctctgctg gcccctcgtt gtggtccacg gtctcgcgac gggcagcgac
     2761 gcgaaagtcg gcttcgtgct catcctcacc gtggtgtgca tcgccgcggt gatcttcgcg
     2821 atctggtggc gcctcgcggt ggactggcgc gaacacaccg gcgtccgagt cggcgccctg
     2881 gtggccagct tcgtcgcccc gctggtcctg gtggcctggc tcaccaccgg tccccttgcc
     2941 gcgggatggg ctcgccacgc cggcaccccc gccaacgtcc tcgcgaagat cggcaccaca
     3001 ccgacggcgc cgagcacacc cgcgccgacc gccaccgcgt cggtcccgac cgccccgttc
     3061 agcgcgtcgc tgaccgggtc gctcacccga tcgaacccga acgcgagcgg gcaagtgacc
     3121 ctccggatcg acaccacgct cagtggaggc gcgctcggcg ccgtcaccgt cgacctcacc
     3181 ggccagcccg tcaacggggg cggcgtgatc ctcgccagca gccaggtcgg cttcgggccc
     3241 atcggagcac ccgcggtcta ccggggctct gtgacgacgc tgcgcgggca gcgcatcatc
     3301 gcgacgatgc gcgcctcggg acggcccacc atcaagctca cgatcaacgt ccaggtcgcg
     3361 tcgtcgggaa gtcgcgtcac cggcacggtc ctcgcccagt agctcagcac ggcccccgga
     3421 ggtgggagcc gatgatgccg gccccggagc gaaagagcca cacggacggg tcgaccggac
     3481 cgaggggctt tccgcggctg ctcgtcggcc gacgtcacgg ctgagcgcct cccggctgag
     3541 tgccgctcag tgcggttcgc accatcggga cgacctcgtc gccgagtccc gcgatctggg
     3601 cccgcgccgc gtcgaacgtc acacccggga cgtggacccg gacgttcagc gcgtcggcgc
     3661 ccgcgacgct cacggcgtcg ccaagctgcc cggcgaccgt gctggcgtcc gccccgcccg
     3721 cgagttcgtc gccgccccag tgcacctgcg cgccacgcgg tgtgtagctc cggtaccggt
     3781 cgagctgctg ctcgacttgg ttccggggcg gttcgccgag ccaggcgcgg cggatcagga
     3841 tgcacgggcc tgtaccgccc gcatcgcggt acgcgtcggt gagctcgcga ca
//
LOCUS       DATCCH010000343         3775 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_204040, whole genome shotgun sequence.
ACCESSION   DATCCH010000343 DATCCH010000000
VERSION     DATCCH010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3775
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204040"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..933)
                     /locus_tag="VIJ44_06510"
     CDS             complement(22..933)
                     /locus_tag="VIJ44_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011779610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HEY5165585.1"
                     /translation="MQVLRTPDEQFESLPGYGFDPHYVDVPDGDGGRLRLHYVDEGPT
                     DGAPILLLHGEPSWSYLYRSMIPVITAAGMRALAPDLVGFGRSDKPAQREDYTYQRHV
                     DWTRSLLDALDVAPLTLVAQDWGGLIGLRLVADRPDRFARVVAANTFLPTGDRDPGDA
                     FRAWQRFSQETPSFDVGRIVSGGCTSDLSPEIVAAYDAPFPDDTYKAGARQFPLLVPT
                     SPDDPASIANRDAWDVLRTWTKPFLTAFSDRDPITAGADKALRAEIPGCEGQPHTTIE
                     GAGHFLQEDRGEQLAGVVCSFVRAHAA"
     gene            complement(1026..1727)
                     /locus_tag="VIJ44_06515"
     CDS             complement(1026..1727)
                     /locus_tag="VIJ44_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEY5165586.1"
                     /translation="MTVEVRRGPERFHTVLPWLDSWHSFSFGGHRDPSNVGHGLLIVN
                     NDDTILGGGGFGEHGHRDMEIVTWVLEGALEHRDSTGTHDVIVPGLAQRMSAGRGIHH
                     SEMNASATDTVHLVQMWVLPDTPGVDPSYQQRDISDALTSGVLVPVASGRGDDGAVTL
                     HQRDAVLWAGQLGSGTTARIPAGPHVHLFVARGDVELEDAGSLTAGDAARLTDAGALT
                     IRAAADAEVLVWETA"
     gene            1840..2631
                     /locus_tag="VIJ44_06520"
     CDS             1840..2631
                     /locus_tag="VIJ44_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165587.1"
                     /translation="MTDEPFRPEAEVTPGLPGAFPSGSAARRGRRAERRRGRRRRITL
                     GVVIIVVVVVAVALVAWPGGNSRSTKTTSRRSTPTSASSTTSTSAVTATTLIPRSSNP
                     AVALAQQYDGRYVGMFTNTTFQTTGPATLDLRIDPNSGVLTASVDLNGDLFGGGAKQV
                     RRIDATVTLGGSNPSLATRTSAFGPVTGKLGNGFSIVFTAPSVPDAKVNTFELTGTLR
                     SDTRGFDATFTVGFRDGRSAQGTVTVLCAPQGQRPSQVTTVCGLM"
     gene            2736..3485
                     /locus_tag="VIJ44_06525"
     CDS             2736..3485
                     /locus_tag="VIJ44_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003875764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5165588.1"
                     /translation="MRFEGKVAVVTGAGRGIGEAYAKALAREGARVVVAELDSEAGER
                     VAKEIEQAGGTAVSVRTDVSSEESTLAMARAGIDAFGGLDYLVNNAAIYGGMELYGLT
                     NMPMDYWDKFMSVNMTGAVLCTRAVFASMAERGGGAVLNQSSTAAWMGAGYYGIAKLA
                     LHGITHSFARELGPMGIRVNALAPGPTDTEATRSTVPAEIIDQLLATMPLARMGTPDD
                     MADACLFLLSDDARWITGHVLNVDGGQIMRV"
     gene            3548..>3775
                     /locus_tag="VIJ44_06530"
     CDS             3548..>3775
                     /locus_tag="VIJ44_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165589.1"
                     /translation="MALAVGLLGLAAFTGPAAASGDTPLRPNGTLFHPPHQARTVTLA
                     STTACQTLLVAGDGDCGVVHATGGDLIFTVEP"
BASE COUNT          565 a         1257 c         1358 g          595 t
ORIGIN      
        1 gttgccgcgg ctcatgggcc gtcaggcggc gtgtgctcgg acgaaggaac acaccacgcc
       61 cgccagctgc tcccctcgat cttcttggag gaagtggccg gctccctcga tggtggtgtg
      121 gggctgaccc tcgcagccag ggatctcggc tcggagcgcc ttgtcggcac cggcggtgat
      181 ggggtcccga tcgctgaacg cggtgaggaa cggcttggtc caggtccgca gcacgtccca
      241 ggcgtcgcgg ttcgcgatcg acgcagggtc gtcgggtgag gtcggtacca gcagtgggaa
      301 ctggcgcgcg ccggccttgt aggtgtcgtc gggaaacggg gcgtcgtagg cggcgacgat
      361 ctcgggtgac aggtcgctgg tgcagccgcc gctcacgatg cgtccgacgt cgaacgacgg
      421 ggtctcctgc gagaagcgct gccaggcccg gaaggcgtca ccggggtcgc ggtcaccggt
      481 ggggaggaag gtgttggcgg cgacgacgcg ggcgaagcgg tcgggtcggt cggcgacgag
      541 tcgcaacccg atgagaccgc cccagtcctg agcgaccagg gtgagcgggg cgacgtcgag
      601 cgcgtcgagg agcgagcgcg tccagtcgac gtgtcgctga taggtgtagt cctcgcgctg
      661 ggcgggcttg tcggaccgtc cgaagcccac caggtcgggt gcgagcgccc gcatacccgc
      721 ggccgtgatg actgggatca tcgagcggta caggtaggac cacgacggct cgccgtgcag
      781 gaggaggatg ggcgctccat ccgtcgggcc ctcgtcgaca tagtgaaggc ggaggcggcc
      841 accgtcacca tccggaacgt cgacgtagtg cgggtcgaac ccgtaccccg gcagcgattc
      901 gaactgctcg tccggtgtgc ggagcacctg catggggtca accttcttcg gggtcagcga
      961 attcggatgt cgaggtcagg gaccgcgccc gatgcctgcg cggcaaccgg ttcagcgcgg
     1021 gcaccctatg cggtctccca gacgagcacc tcggcgtcgg ccgcggcccg gatcgtgagc
     1081 gcgccggcgt cggtcaggcg ggcggcgtcg ccggcagtga gcgagcccgc atcctcgagc
     1141 tcgacgtcgc cgcgggccac gaagagatgg acgtggggcc cggcgggtat tcgagccgtc
     1201 gtgcccgaac cgagttgtcc ggcccacagg acggcgtcgc gttgatgcaa ggtgaccgcc
     1261 ccatcgtcgc ctcgacccga ggcgaccggg accaggacac cggaggtcaa cgcatcggag
     1321 atgtcgcgct gctggtacga cggatcgacg cccggggtgt caggcagcac ccacatctgg
     1381 acgaggtgga cagtgtccgt cgcgctcgcg ttcatttccg aatggtggat ccctcgaccg
     1441 gccgacattc gctgcgcgag gcctgggacg atgacgtcat gggtaccggt gctgtcgcga
     1501 tgctccaacg caccctcgag gacccaggtg acgatctcca tgtcgcgatg cccgtgttcg
     1561 ccgaacccgc cgcctccgag aatggtgtcg tcgttgttca cgatcaagag gccgtgtccg
     1621 acgttcgaag ggtcgcgatg ccctccgaag ctgaagctgt gccacgagtc gagccacggg
     1681 aggacggtgt gaaaccgctc gggtccccgg cgtacctcga cggtcaccgg gtgattatcg
     1741 tcgcccgcgc cactggatgc gacaccagcc gacgtggttg tggcaccggg tgcgtcgcac
     1801 gacactggtg gcggggtcgg ctggcgttac cctcccgggg tgacggacga gccatttcgt
     1861 cccgaggccg aggtgacgcc cggcctgcca ggcgcattcc cgtcggggtc ggccgcgcga
     1921 cgtggccgtc gcgccgaacg gcgccgaggg cggcgtcgcc ggatcacgct cggcgtcgtc
     1981 attatcgtcg tcgtggtggt cgcggtcgcg ctggtcgcct ggccgggcgg aaacagccgt
     2041 tcgacgaaaa cgacgagccg tcgcagcacc ccgacgtcgg cgtcatcgac gacgtcgacc
     2101 tcggcggtca ccgcgacgac gctgatcccg cgctcgtcta acccggcggt cgccttggcc
     2161 cagcagtacg acggccgcta cgtcgggatg ttcaccaaca cgacgtttca gacgaccggg
     2221 cccgccacgc tcgacctgcg gatcgacccc aactcggggg tgctgaccgc gtcggtggac
     2281 ctcaatggcg acctcttcgg gggcggcgcc aagcaggtcc gccgcatcga cgccactgtc
     2341 accctcggtg gctcgaaccc gtcgctggcg acccgcacat cggcgttcgg gcccgtgacc
     2401 gggaagctcg ggaacgggtt ctccatcgtg ttcacggccc cgagcgtccc tgacgccaag
     2461 gtcaatacgt tcgagttgac cgggacgttg cgttccgaca ctcggggctt cgacgcgacc
     2521 ttcaccgtcg gctttcgtga cggtcgctcc gcccagggca cggtgaccgt cttgtgcgca
     2581 ccccaaggtc aacggccgag ccaggtcacg acggtctgcg gcctgatgta gccgaccggc
     2641 tcggtggctc ccgcctggga ccagggccga acgcgggctg cccgccggtg ggcggctcgg
     2701 tagggtcggc cagccaatcg aatgggaggg ctctgatgcg cttcgagggc aaagtagcgg
     2761 tcgtcacggg ggccggtcgt ggaatcggtg aggcgtacgc gaaagcgctc gcgcgcgagg
     2821 gggcccgggt cgtcgtcgcc gagctcgact ccgaagccgg cgagcgggtc gcgaaggaga
     2881 tcgagcaggc ggggggtacg gcagtctcgg tccgtaccga cgtctcgagc gaggagtcga
     2941 cgctcgcgat ggctcgggcg ggtatcgacg ccttcggtgg gctcgactac ctcgtcaaca
     3001 acgcggcgat ctacggcggg atggagctct acgggctgac gaacatgccc atggactact
     3061 gggacaagtt catgtccgtg aacatgaccg gcgcggtgct ctgcacccgg gcggtgttcg
     3121 catcgatggc cgagcgcggc ggtggcgcgg tcctcaacca gtcgtcgacc gcggcgtgga
     3181 tgggagccgg gtactacggc atcgcgaagc tcgcgttgca cggcatcacc cactcattcg
     3241 cccgggagct cggaccgatg ggaatccgag tcaacgccct cgcgcccgga ccgaccgaca
     3301 ccgaagccac ccggtcgacg gtcccggccg agatcatcga ccagctgttg gcgacgatgc
     3361 cgctcgcccg catgggaacc cccgacgata tggccgacgc ttgcctcttc ctcctttccg
     3421 acgatgcgcg ctggatcacg ggccacgtcc tcaatgtgga cggcggccag atcatgcggg
     3481 tgtgaggcac ggtcgccgac ccgccgtcgg cagcctctcc gtgttgcatt cctggtcccg
     3541 tctcacggtg gcgctcgcgg tcgggctgct cggcttggcg gcattcaccg gtcccgccgc
     3601 cgcttcgggt gacacgccgc tccgcccgaa cgggacgctg ttccatcccc cacaccaggc
     3661 ccggacggtc acgctggcgt cgaccaccgc gtgccagacg ctgcttgtcg ccggggacgg
     3721 cgactgtggt gtcgtgcacg ccaccggcgg tgacctgatc ttcaccgtcg aaccc
//
LOCUS       DATCCH010000344         3943 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_577271, whole genome shotgun sequence.
ACCESSION   DATCCH010000344 DATCCH010000000
VERSION     DATCCH010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3943
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577271"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..169)
                     /locus_tag="VIJ44_06535"
     CDS             complement(<1..169)
                     /locus_tag="VIJ44_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018092413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5165590.1"
                     /translation="MGTVSGRWVITAAVLGSGVAFLDGTVVNVALPSIGRDLHTDVAG
                     LQWTLDAYLVTL"
     gene            complement(205..501)
                     /locus_tag="VIJ44_06540"
     CDS             complement(205..501)
                     /locus_tag="VIJ44_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HEY5165591.1"
                     /translation="MADTCVHLGDLATDSAPGGPGCYECTLMGATWVHLRRCVECGHI
                     GCCDSSPNRHATKHFHGTEHPLIQSYEPGEDWLWCYVDELMFEVPGHSRSPSHP"
     gene            complement(515..832)
                     /locus_tag="VIJ44_06545"
     CDS             complement(515..832)
                     /locus_tag="VIJ44_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165592.1"
                     /translation="MSDEPATVLEAVRLLEAEGFDTSFSLTAEGLKCGRCGRAEAIER
                     AEVLRVYRFEGPSDPDEEAIVYGLRCPGCDQLGTLVSSFGPSADPELTDRLVMLDARF
                     DGD"
     gene            897..1685
                     /locus_tag="VIJ44_06550"
     CDS             897..1685
                     /locus_tag="VIJ44_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020630302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5165593.1"
                     /translation="MLLDRFRLDGLAAVVTGAGRGIGAGAARALAEAGADVVLTARTP
                     EQLEVVAADIRALGRQALVVPGDVNDLDHIDRLVDATITEFGHLDIVVNNAGGSMPAP
                     LLDTSVRSFEQAFHFNVTTAFALTKAALPHLLAGERGAVVNISSAMGRLRDRGFVAYG
                     TAKAALAHMTRLMAADCAPRVRVNAIAVGSVATSALEIVLTNDPLRDEMVEHTPMRRL
                     GEVEDIAIGVLYLASDASSYVTGKVIEIDGGIEAPNLDLRLPDL"
     gene            1724..1796
                     /locus_tag="VIJ44_06555"
     tRNA            1724..1796
                     /locus_tag="VIJ44_06555"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1757..1759,aa:Arg,seq:tct)
     gene            2163..2239
                     /locus_tag="VIJ44_06560"
     tRNA            2163..2239
                     /locus_tag="VIJ44_06560"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2197..2199,aa:His,seq:gtg)
     gene            complement(2232..2993)
                     /locus_tag="VIJ44_06565"
     CDS             complement(2232..2993)
                     /locus_tag="VIJ44_06565"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEY5165594.1"
                     /translation="MALRVWVDTDVGDDPDDAIALLCAVGHPGIDLVGVSTVDGDHDR
                     RVRAARALVDAPVHAGDEPALADTVRAAAPDAVLAIGPLTNVAALLAAGPIPPVTLMG
                     GALAPVRHWGMTIETEHNFGRDPRAAATVLEAADPLVVPLDVTVSTCLNRDTLGRLVA
                     AAPSLREPTERWLALQRDVGVPVEERSVCLHDPLALLALVEPALVHVETRTVQVEHDG
                     RLVDDPRGTPCRIVSEADVQLIIELVLGFLAQNLG"
     gene            complement(2994..3911)
                     /locus_tag="VIJ44_06570"
     CDS             complement(2994..3911)
                     /locus_tag="VIJ44_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HEY5165595.1"
                     /translation="MEVLVTGASGFIGSAVRPALISAGHRPIIAVRGREVPRGVDGIA
                     WDPEAGTVDVQALEGVGGVVHLAGAGIGDRRWTPSRKQLIVESRTKSTGLLVSTLVGL
                     GRKPSVLVSSSAVGYYGDRGDEVLTEDSGPGEDFTAQVCRQWEAAAAPVTGADIRLVT
                     IRTGIVLGRAGGMLARVVAPFRMGLGGRLGSGRQYLSWVSLDDEVAVIVRALEQSALT
                     GATNVTAPAPVTNAEFTDTLGRVLHRPTRVPTPLTPLRAVYGRELVESLLLGGQRVLP
                     AALTAGGFEFAHPTLEGALRAALDRPESS"
BASE COUNT          637 a         1418 c         1297 g          591 t
ORIGIN      
        1 tcagggtcac gagatacgcg tcgagggtcc actgcagccc cgcgacatcg gtgtgcaggt
       61 cacgcccgat cgacgggagc gcgacgttga cgaccgttcc gtcgaggaac gcgacccccg
      121 agccgagcac cgccgcggtg atgacccacc ggcccgacac ggtccccatg cggagtcccg
      181 cccctgaccc gggaccagag gtgctcaagg gtgtgacggg ctgcgggaat ggcccgggac
      241 ctcgaacatc agctcgtcga cgtagcacca cagccagtcc tctcccggct cgtaggactg
      301 gatcaggggg tgctccgtgc cgtggaagtg cttcgtcgcg tgacggttgg gggaactatc
      361 gcaacacccg atgtgaccgc attcgacaca gcggcgcaga tgcacccacg tcgcgcccat
      421 gagcgtgcac tcgtagcaac cgggcccgcc cggtgccgag tcggtggcca ggtcgccgag
      481 atgtacgcag gtgtctgcca cccggagggt ctcctcagtc gccgtcgaag cgggcgtcga
      541 gcatcaccag ccggtcggtg agctcggggt cggcactcgg tccgaacgac gagacgagcg
      601 ttccgagctg atcgcatccc gggcagcgca gcccgtagac gatggcctct tcgtcggggt
      661 cgctcggccc ctcgaagcgg tagacccgca gcacctcggc ccgttcgatg gcctcggcgc
      721 gcccgcaccg accgcacttc aggccctccg cggtcagtga gaacgacgta tcgaaccctt
      781 cggcttccag caaccgcacg gcttcgagca ccgtcgccgg ttcgtcgctc acggtctcga
      841 ccctaccgtc aggttcgggg cgttcattcg cccgacgggg gctagcgtgc cgccccgtgc
      901 tgctcgaccg tttccgactc gacgggctcg ccgcggtcgt gaccggagcc gggcgcggga
      961 tcggggccgg cgcggcccgg gccctggccg aggccggcgc cgacgtcgtg ttgaccgccc
     1021 gcacccccga acagctcgaa gtcgtcgcag ccgacatccg ggccctcggc cgtcaggctc
     1081 tcgtcgtacc cggcgacgtc aacgacttgg accacatcga ccggctcgtc gacgccacga
     1141 tcaccgagtt cgggcacctc gacatcgtcg tcaacaacgc gggtggcagc atgccggccc
     1201 cgttgctcga cacctcggtc cggtcgttcg agcaggcctt ccactttaat gtgaccaccg
     1261 cgttcgcgtt gacgaaagcg gcgctccccc acctgctcgc cggcgaacgg ggcgcggtcg
     1321 tcaacatcag ctcagcgatg ggtcgcctgc gcgaccgcgg cttcgtcgcg tacggcaccg
     1381 ctaaagccgc gttggcccac atgacccggc tcatggccgc cgactgcgca ccccgggtgc
     1441 gggtcaatgc catcgcggtc gggtccgtcg cgacctctgc cctcgagatc gtcctgacca
     1501 acgatccgct ccgcgacgag atggtcgaac acacaccgat gcgacgcctc ggtgaagtcg
     1561 aggacatcgc catcggtgtc ctctacctcg cttccgacgc gtccagctac gtgaccggga
     1621 aagtcatcga gatcgacggt ggcatcgagg cacccaacct cgaccttcgt ctccccgatc
     1681 tctaagcagc caggtacact tagcccggcc cggcatccat cgagcgcccg tagctcagag
     1741 gacagagcga cggacttcta atccgttggt cgggggttcg aatcctcccg ggcgcgcgac
     1801 cggcgttctc aagccgtctc tcgcaggtac acgaccgatc gcagtctcga ccagccggtt
     1861 tcaagccctc cagtcgaccc ccgtgcacct gatccactcg taccgatcac gaagatcgag
     1921 caagcggccc ccggcgtcgc caaatacgcg tggtgcggcg agctcgcagg ccagaccctg
     1981 acgactcgcg atctcgaaga ccgggagtcc tcttccccag cggcgagttc gcactcaagt
     2041 cgctagcatc gatcgcgctc caggagctcc acccgagcga ccttcggtcg tcgaatccgt
     2101 ttgggtcgtg ggttcgaacc cttctgggcg cgcacccggc ggctagctgc gccggagcca
     2161 tggtggccgt agctcagttg gctagagcgc cgggttgtgg tcccggaggt cgggggttcg
     2221 aaacccctcg gtcaccccag attctgggcc agaaacccga gtaccagttc gatgataagt
     2281 tgcacgtccg cctccgaaac gatccgacac ggggtccctc gcgggtcgtc gacgagacgt
     2341 ccgtcgtgct cgacctgcac ggttcgagtc tcgacgtgta ccagtgcggg ttcaacgaga
     2401 gcgagcagcg cgagcggatc gtggaggcac actgatcgct cctcgaccgg gacaccgacg
     2461 tcccgctgca acgcgagcca tcgctcggtc ggttctcgga gggacggcgc cgcggcgacg
     2521 agacggccga gtgtgtcgcg gttgaggcag gtcgacacgg tcacgtcgag gggcacgaca
     2581 agcgggtcgg cggcctcgag cacggttgca gcggcccgcg ggtcgcgccc gaagttgtgc
     2641 tccgtttcga tggtcatgcc ccagtgccgg accggtgcca gcgcgccccc catgagcgtc
     2701 acgggcggga tcggcccggc ggcgagaagc gccgcaacgt tggttagcgg tccgatcgcc
     2761 aagacggcgt ctggcgccgc tgcccggacg gtgtccgcga gtgcgggctc gtcgcccgcg
     2821 tgcaccggag cgtcgacgag ggcccgggcg gcgcgaaccc ggcggtcgtg gtcgccgtcg
     2881 accgtgctca ccccgaccag gtcgattccc ggatgtccga ctgcgcagag cagggcgatg
     2941 gcatcgtcgg ggtcgtcacc cacgtcggtg tcgacccaga cccgcagcgc cactcaggaa
     3001 ctctccggac ggtccaacgc cgcccgtaac gcgccctcga gtgtggggtg ggcgaattcg
     3061 aagccgcccg cggtcagcgc cgcgggtagg acccgctgtc cgccgaggag caggctctcc
     3121 accagctcgc gtccgtacac agcgcggaga ggcgtcaacg gcgtgggcac gcgcgttggt
     3181 cggtgcagca cccgacccaa cgtgtcggta aactcggcgt tcgtgacggg agccggtgcc
     3241 gtgacgttgg tcgcgccggt cagcgccgac tgttccagcg ctcggacgat caccgcgacc
     3301 tcgtcgtcga gcgagaccca gctgaggtat tgccgtccgg agccaagacg acctccgaga
     3361 cccatacgaa acggggccac gacccgcgcc agcatcccgc ctgctcgccc gagcacgatc
     3421 ccggttcgga tcgtgaccag ccggatgtca gcacccgtca ccggggccgc cgccgcctcc
     3481 cactgccgac agacctgggc cgtgaagtcc tcgccggggc cgctgtcctc agtgagcacc
     3541 tcgtcgccgc ggtcgccgta gtagccgacc gctgaggacg acaccaagac cgaaggcttg
     3601 cggcccagcc ccacaagggt cgaaacgagc agccctgtcg acttcgttcg gctctcgacg
     3661 atcagctgct tgcgcgaggg ggtccatcgc cggtcaccga tcccagcacc ggcgagatgg
     3721 accacgccgc cgacaccctc caaagcttgg acgtcgacgg tgccggcttc ggggtcccaa
     3781 gcaatcccgt cgacaccccg cggcacctcg cggccgcgca ccgcgatgat cggacggtga
     3841 ccggcggaga tcagggctgg gcgcaccgcg gacccgatga agccgctcgc gccggtgacg
     3901 agaacctcca cggtcccgca ccgtagatcg caaagccggt cga
//
LOCUS       DATCCH010000345         3335 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_351354, whole genome shotgun sequence.
ACCESSION   DATCCH010000345 DATCCH010000000
VERSION     DATCCH010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351354"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="VIJ44_06575"
     CDS             <1..683
                     /locus_tag="VIJ44_06575"
                     /inference="COORDINATES: protein motif:HMM:NF027034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HEY5165596.1"
                     /translation="LGRNEVFAAARRLAGDVDALHLDGAAVTAEEVRDAVAVAAGPFN
                     GARVAIDPDRVLAAATRAGTRLGAVVAAGGRVAFATAQPASLLPLYTALARASLAAGA
                     TVVEVDRAGPYASGRSLWWHDGVAVATDGDQLCTESHGAAGAEWLFAVGRPAVAVADG
                     VFAVAAIGAGLDTIAFADLDAPAPALAAHRGRPVDVVTVGGHRPPGAYRPVRDALLEA
                     LAGPRHAQ"
     gene            771..974
                     /locus_tag="VIJ44_06580"
     CDS             771..974
                     /locus_tag="VIJ44_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY5165597.1"
                     /translation="MSDVAQSFAEARFLTVQEVADLMRVSSMTVYRLIKAGDLPAVRV
                     GRSFRVRDVDVNTYLSSRYTQAG"
     gene            999..1823
                     /locus_tag="VIJ44_06585"
     CDS             999..1823
                     /locus_tag="VIJ44_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HEY5165598.1"
                     /translation="MKPVAFLSDYGLADEFVGVCKGVLHGLAPGVTVIDVTHDVPAHD
                     VRSGALTLVRAVQYLPEDCVVMAVVDPGVGTDRRLLAVECDHATLLGPDNGLLAPAVA
                     LLGGARRVVALTSPEHRLPAPGPTFAGRDVLAPAAAHLAAGHPLDELGEPCDPASLVP
                     GLVNLPTTLDGGALEAQVWWVDHFGNAQLNVGPDELRAAGAEPGSTIEVRSGDWAKAA
                     RWVETYADARPSELVLLVDSYGLVALALDRRPAATEYHLQPGVVVVLAPPGTTVPA"
     gene            2191..>3335
                     /locus_tag="VIJ44_06590"
     CDS             2191..>3335
                     /locus_tag="VIJ44_06590"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY5165599.1"
                     /translation="MNLADGLLASAATHPDAVALIGRDGTATTYAELAETVARLGTLL
                     GDAGVNPDDRVAVVSLNNEAFVAAYLATLHVGAVCVPLNPAAPPAGLERELTDIDPAL
                     TLAAGSSTSLVAAAGRDVHPIDLGSLPAAAASRVDRAPDDLAVLLFTSGTAGTPRAAR
                     LTHANLAANISQVQDHPGLRLEPGDVGMALLPGFHIFGLNVVLGVGLAAGATTVLVER
                     FNAAESARLARDRGVTVLAGVPAMFRDWTDLDPADVSPDTFASVRLAVSGAAPLPAEV
                     AASFHDRFGIVLHQGYGLTEASPIVTTTALSVGALRPGSIGPPLPGVHVRLVDSTGAD
                     VLAGDPGELWVQGPNVFPGYWHDDEASAQALTSDGWLRTGDVGVIED"
BASE COUNT          412 a         1355 c         1100 g          468 t
ORIGIN      
        1 gcctcggtcg gaacgaggtc ttcgccgccg cgcggcgact ggccggcgac gtcgacgcgc
       61 tccacctcga cggggctgcc gtcacggccg aagaggtccg cgacgcggtg gcggtcgcgg
      121 ctggaccctt caacggggcg cgggtggcga tcgaccctga ccgggtgctc gccgccgcga
      181 ccagggccgg aacccgcctc ggtgcggtcg tcgccgccgg cggacgagtc gcgttcgcga
      241 ccgcgcagcc cgcgtcgctg ctgccgctgt acaccgcgtt ggcgcgggcc agcctcgccg
      301 ccggtgccac tgtggtggag gtcgaccgcg ccggtccgta cgcgtcgggt cgttccttgt
      361 ggtggcacga cggtgtcgcg gtcgccaccg acggcgacca gctttgcacc gagtctcacg
      421 gcgcggccgg cgccgagtgg ctcttcgcgg tcggacgccc ggccgtcgcg gtcgccgacg
      481 gtgtgttcgc cgtcgccgcc atcggcgccg ggctcgacac catcgccttc gccgacctcg
      541 acgcgccggc cccggccctc gccgctcacc ggggccgccc ggtcgacgtc gtcaccgtcg
      601 gtggtcatcg accccccggg gcgtaccgcc cggtgcgaga cgcgctgctc gaggccctgg
      661 ccgggcctcg ccacgcccag taacccccga cagcaagggc aggggccagc cgccacactt
      721 ggcaacagag accccggggc cctacgctgc ccccgagagc ggggagaggg gtgagcgacg
      781 tggcgcagtc ttttgcggaa gcgcgcttct tgacggtcca agaggtggcc gacctgatgc
      841 gggtgtcgtc gatgaccgtg taccggctga tcaaggcggg cgacctgccg gcggtgcgcg
      901 tcggccggtc gtttcgggtc cgggacgtcg acgtcaacac ctacctgtcc tcgcggtaca
      961 cccaagccgg gtagcgacac gtcgaccacg cgacgctgat caaacccgtc gccttcctct
     1021 cggactacgg actcgccgac gagttcgtcg gggtctgcaa aggcgtgctg cacgggctcg
     1081 ctcccggcgt caccgtcatc gacgtcaccc atgacgtccc cgcccacgac gtccgctccg
     1141 gcgcgctcac cctggtgcga gccgtccagt acctccccga ggattgcgtc gtgatggccg
     1201 tcgtcgaccc cggtgtgggg accgaccgac ggctcctcgc cgtcgaatgt gaccacgcga
     1261 ccctcctcgg gcccgacaac ggcctgcttg cacccgcggt cgcgctgctc ggcggcgccc
     1321 gtcgcgtcgt cgcgctcacc agccccgagc accgtctccc agcgccggga cccacgttcg
     1381 ccggccgcga cgtcctcgcc cccgccgccg cgcacctcgc cgccggtcac ccgctcgatg
     1441 agctcggtga gccgtgcgac ccggccagcc tcgtccccgg cctcgtcaac ctgcccacca
     1501 ccctcgacgg cggcgccctc gaggcccagg tgtggtgggt cgaccacttc gggaacgcgc
     1561 agctgaacgt cggacccgac gagctgcggg ccgccggtgc cgaacccggc tccaccatcg
     1621 aggtccgcag cggcgactgg gccaaggcgg cgcgctgggt cgagacgtac gccgacgcac
     1681 gcccctcaga gctcgtgctg ctggtcgact cctacggcct ggtcgcgctg gcgctcgacc
     1741 ggcgccccgc ggccaccgag taccacctcc aacccggtgt ggtggtggtc ctcgcgcccc
     1801 ctggcactac ggtgccggcg tgaggccggg gaccacgatc gccatcgcgg cgctgctcat
     1861 cgtgatcttc gccgcggcag tggcccagtt cgtcctccgg ctgggcccct gagcggaatc
     1921 tcaccctccg tgaccgaatc ctctcaaggc ggccacagag cgagacgatg cagggagcgg
     1981 ttcccggtat gcgggcgtgg gtagcgtgac gccacggcaa agtggcgttc actggcctcc
     2041 cagcccgccc caagacccac gcccgctgcc ggagccatcc tctcgcccct ccccgctcgc
     2101 cacgccgcga gcaggcgaga cggaccggag gtcaccctgg gctcgtccgg agcggccggg
     2161 cccggcgggt ccgcacctac catccggcgc atgaacctgg ccgacgggct gctcgccagc
     2221 gccgcgaccc accccgacgc cgtcgcgctc atcggtcggg acggcacggc cacgacgtac
     2281 gccgagctcg ccgagaccgt ggcccggctg ggcacgctcc tcggcgacgc cggtgtgaac
     2341 cccgacgacc gggtcgccgt cgtcagcctc aacaatgagg ccttcgtcgc ggcttacctg
     2401 gccactctcc acgtcggagc ggtctgtgta ccgctcaacc ccgctgctcc ccccgccggc
     2461 ctcgaacgcg agctcaccga catcgacccc gcgctcaccc tcgccgccgg atcgtcgacg
     2521 tcgctcgttg ccgccgccgg ccgcgacgtc cacccgatcg acctcgggtc gctccccgcc
     2581 gccgccgcct cccgtgtcga tcgagccccc gacgatctcg cggtcctgct cttcacttcc
     2641 gggaccgccg ggacccctcg cgccgcgcgg ctcacccacg ccaacctcgc ggccaacatc
     2701 agccaggtcc aggaccaccc gggacttcgc ctcgaacccg gcgacgtcgg catggccctg
     2761 ctccccggct tccacatctt cgggctcaac gtggtcctcg gcgtcggcct cgccgccggt
     2821 gccaccaccg tcctggtcga gcgcttcaac gcggccgaaa gtgcgcggct cgcccgcgac
     2881 cgcggggtca cggtcctcgc cggcgtcccc gccatgttcc gcgactggac cgacctcgac
     2941 cccgccgacg tgtcgcccga cacgttcgcg tcggtgcgcc tggcggtgtc gggggcggcg
     3001 cccctcccgg cggaggtcgc cgcctcgttc cacgaccggt tcggcatcgt cttgcatcag
     3061 ggctacggcc tcaccgaagc ctcaccgatc gtcaccacca ccgccctcag cgtcggagcg
     3121 ctccgccccg gatcgatcgg cccacccctg cccggggtcc acgtccgcct ggtcgactcc
     3181 acgggcgccg atgtcctcgc cggcgacccc ggcgagcttt gggtccaggg ccccaacgtc
     3241 ttccccgggt actggcacga cgacgaggcg agcgcccagg ccctcaccag cgacggctgg
     3301 ctccgcaccg gcgacgtcgg ggtcatcgaa gatga
//
LOCUS       DATCCH010000346         2367 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_600032, whole genome shotgun sequence.
ACCESSION   DATCCH010000346 DATCCH010000000
VERSION     DATCCH010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600032"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="VIJ44_06595"
     CDS             <1..410
                     /locus_tag="VIJ44_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEY5165600.1"
                     /translation="PAVESLPADDGPGELVERQVPVEVDGKRFSVRLWLPEASGPAAT
                     PAAGAPRRAPRPKTAATGGGGNGTIAAPMQGTIVKLLVSEGDDVEAGQAVLVLEAMKM
                     ENHVNTERGGTVKEIRVQAGDTVGTGDILVVIG"
     gene            complement(450..1811)
                     /locus_tag="VIJ44_06600"
     CDS             complement(450..1811)
                     /locus_tag="VIJ44_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY5165601.1"
                     /translation="MATTRLVIIGGGPAGNTCATVAASLGADVTLVERDVIGGAAHLW
                     DCIPSKALIATGGELAELTRAPTMGLKAEGALDIAALRRRVEAIQGHLHDQLCSLLTS
                     QGVRLVVGAARFKGPHTIVAETATGLEEFEADAVLVATGSRPRVPDFAPVDGERVLTT
                     RQSYPPGEIPEHAIVIGSGVTGTEFTHMFASLGSRVTLLVSRQQVLPIKDAEVASVLE
                     DAFLERGVTLLKGARATSITRDGDTVRVGCEDGRVIDGTHAILAVGSIPNSDGVGLAE
                     AGVDVDDAGYVPVNHNCLSNVTHIYAAGDVSGKLPLSSVAAMQGRKIAEHLMGLHNRP
                     HRHLDYDKAASAVFTEPEIADVGLAEAEAFASGRKIRVTKVPFTANAKALIKGDPRGF
                     VKIISDPATGVVLGGSIVGRNAAELIGVLAIAAANGLKVADIVDSLLVHPTLSESLAE
                     AAS"
     gene            1902..>2367
                     /locus_tag="VIJ44_06605"
     CDS             1902..>2367
                     /locus_tag="VIJ44_06605"
                     /inference="COORDINATES: protein motif:HMM:NF013269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HEY5165602.1"
                     /translation="MPELRRDRLTGEVVLLAPGRAARPHTVLGMPEGSTDATRCPFCP
                     GHESDTPPEVARVGDGPPDGPGWRVRAFPNLYPIVGGAEAGDGATGDHEVVVLSPDHL
                     RDLGALDDDQMIDVFRVLRDRSRAHAQAGRAYVQIAVNHGRDAGASIEHPHAQ"
BASE COUNT          353 a          844 c          837 g          333 t
ORIGIN      
        1 cccccgcggt cgagtcgctt cccgctgatg acggtcctgg cgagctggtg gaacgccagg
       61 ttccggtcga ggtcgatggg aagcggttct cggtgcggct ctggctcccg gaggcgtccg
      121 gacccgcggc gacgccagcc gcaggcgcgc cccggcgcgc tccccggccg aagaccgcgg
      181 ccaccggcgg cggcgggaac ggcaccatcg ccgcgcccat gcaaggcacg atcgtcaagc
      241 tgttggtcag cgaaggcgac gacgtggagg ccggtcaggc ggtgctcgtc ctcgaggcca
      301 tgaagatgga gaaccacgtc aacaccgagc ggggcggcac cgtcaaggaa atccgggtcc
      361 aagcgggcga taccgtcggg accggcgaca tcctcgtcgt catcggctga tcccccacca
      421 gtcggcctcg cggccaagcg ccggaacgct caggacgcgg cttcggcgag ggactccgac
      481 agtgtcgggt gcaccagcag cgaatcgacg atgtcggcga ccttcagccc gttggccgca
      541 gcgatggcga ggaccccgat gagctcggcg gcgttgcggc cgacgatcga cccaccgagg
      601 acgacgccgg tcgccgggtc cgagatgatc ttgacgaagc ctctcgggtc gcccttgatc
      661 aacgctttgg cgttggcggt gaacggcacc ttcgtgaccc ggatcttgcg tcccgacgcg
      721 aacgcctccg cctcggccag gccgacgtcg gcgatctcgg gttcggtgaa caccgccgag
      781 gctgccttgt cgtagtcgag gtggcggtgc ggccggttgt gcaaccccat caggtgctcc
      841 gcgatcttgc ggccctgcat ggccgccacc gacgacaacg gcagcttgcc cgacacgtcg
      901 ccggcggcgt agatgtgcgt cacgttcgac aggcagttgt ggttgaccgg cacgtacccg
      961 gcgtcgtcga cgtcgacccc ggcttcggcc aaaccgaccc cgtcactgtt ggggatcgac
     1021 ccgaccgcga ggatggcgtg cgtcccgtcg atgacccggc cgtcctcaca cccgacacgc
     1081 accgtgtcac cgtcgcgggt gatgctggta gcccgggcac ccttgagcaa cgtgacgccc
     1141 cgctcgagga acgcgtcctc gagcaccgag gcgacctcgg cgtccttgat gggcagcacc
     1201 tgctgacgtg acacgagcag cgtcacccgc gatccgagcg acgcgaacat gtgggtgaac
     1261 tcggtgccgg tgacaccgga gccgatcacg atggcgtgct cgggaatctc gccgggggga
     1321 tacgactggc gtgtggtgag cacccgctca ccgtcgacgg gcgcgaagtc agggacgcgg
     1381 ggccgggacc cggtggccac gagcaccgcg tcggcctcga actcctcgag gcccgtggca
     1441 gtctcggcga cgatggtgtg cggccccttg aaccgggctg cgccgaccac cagccggacc
     1501 ccctgagagg tgaggaggga gcagagctgg tcgtggagat gcccttggat ggcttcgacg
     1561 cggcggcgca acgcggcgat gtcgagcgct ccctcggctt tgagccccat ggtcggggct
     1621 cgggtcagct cggcgagctc acctccggtg gcgatgagcg ccttcgaggg aatgcagtcc
     1681 cagaggtgcg cggcgccccc gatcacgtca cgctccacga gggtgacgtc cgcgccaagg
     1741 gacgcggcca ccgtcgcgca cgtgttgccg gccggtcccc cgccgatgat caccaatcgg
     1801 gtcgtcgcca tcggcccagg gtacggcgaa tgccaacgcg cttcggcggc aggtgaggcg
     1861 ccggtcgcgg tccgaccgtc gggcgcccct acgctccggg cgtgcccgag ctgcgccgcg
     1921 accgactgac cggcgaggtg gtgctcctcg ccccaggacg ggccgcgcgg ccccacaccg
     1981 tcctcggcat gcccgagggg agcaccgacg caacgcgctg cccgttctgt cccggtcacg
     2041 aatccgacac gccaccggag gtcgctcggg tcggcgacgg cccacccgac ggtcccggct
     2101 ggcgggtccg ggcgttcccg aacctctatc cgatcgtcgg cggggccgag gcgggcgacg
     2161 gcgccaccgg cgaccacgag gtcgtggtcc tctcccccga tcatctccga gacctcgggg
     2221 ccctcgacga tgaccagatg atcgacgtgt ttcgcgtgct ccgcgaccgg tcgcgtgccc
     2281 atgcccaggc cggccgcgcc tacgtgcaga tcgcggtgaa ccacggccgc gacgccggag
     2341 cgtcgatcga gcatccccac gcccagg
//
LOCUS       DATCCH010000347         2085 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_600204, whole genome shotgun sequence.
ACCESSION   DATCCH010000347 DATCCH010000000
VERSION     DATCCH010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600204"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="VIJ44_06610"
     CDS             complement(<1..250)
                     /locus_tag="VIJ44_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEY5165603.1"
                     /translation="MSAQELAEAVVLGDLTLALCLVGHLVPLASALFAAAVVPMAAVA
                     ARHRVRAVLAGGIAASVVAFLVAGTGLVVSVAGCTVLGA"
     gene            complement(573..1511)
                     /locus_tag="VIJ44_06615"
     CDS             complement(573..1511)
                     /locus_tag="VIJ44_06615"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEY5165604.1"
                     /translation="MDKSTARPLRSVLFVPGDQVDQVDQALRSGADSIVVDLEEPRTP
                     FPEAARHTARTGMRAFFDGLAPDADRPVLFARVQPPSSGQTLKDLRAVMGTAVAGVLV
                     PKVQGPEDIHAMHALLHCMEVELDLPVGSTAIYPILETAQALRRAYEIGVASPRVSYL
                     GGAVSRFGDIHQALGYRWTPEGRETLFLRSKVLVDARAAGIRYPVSGMWGGGRDDVDG
                     LRAWAEQLRDLGYYGMMLGDPVHIPIVHEVFTPSAADIKYWRDLDRLARDAETTGEGP
                     ITYGDAGQGEGHVVHIAHVGSARLNLQWARDLGIVT"
     gene            complement(1533..>2085)
                     /locus_tag="VIJ44_06620"
     CDS             complement(1533..>2085)
                     /locus_tag="VIJ44_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEY5165605.1"
                     /translation="GLVGHWENRNGDAISGLDPDLLDVDELLRYFGDYGDPSVLGRLD
                     DLRRYYGRPAARPDLLDEFAACEIHEADDLRRLFEPHFYFGCEADDPLLVWAFRDDIN
                     PLGARLRPVLGSDISHWDVPDMTEPIAEAFELVERDLLTENQFRELTFLNPVRLHGAA
                     NGAFFAGTVCETAAADALRTEPA"
BASE COUNT          358 a          734 c          710 g          283 t
ORIGIN      
        1 ccgcaccgag cacggtgcag ccggcgaccg aaacgacgag ccccgtgccg gccacgagga
       61 acgcgacgac cgacgccgcg atcccgcccg ccagcaccgc ccggacgcga tggcgggcgg
      121 cgaccgcagc catcgggacg acggcagcag cgaacaaggc cgaagccaac gggaccagat
      181 gcccgacgag gcacaacgcc aacgtcagat cacccaagac gaccgcctcg gccagctcct
      241 gcgcgctcaa ccggccgcgc cgtcggacag ggtccgcggg aggcgccaca ccagtttggt
      301 ccacgtccga agccttccac gcggaaccgg gactcatgtc gcgcggccac ggcccgaggg
      361 accgtgagca ggtcgagata ccgcccaggc tccgccggac gagtcggcgc gccgcgactc
      421 acctgacctc tcagccttgt cacagggcgg ttgcgggatg ctggccccgc tcccggtggc
      481 cgcgccacct gaccctggac gccgacggcg cggtcaccgg ggtgcgcaaa ccgaccacgc
      541 gaacgcgtcg cgcgttcccg agaaccggtg ggctacgtga cgatcccgag atcgcgtgcc
      601 cactgcaagt tgaggcgagc cgaaccgacg tgcgcgatgt gcacaacgtg gccttcgcct
      661 tgcccggcgt cgccgtaggt gatcggtcct tcgcccgtgg tctcggcgtc ccgagccagc
      721 cggtcgagat cccgccaata tttgatgtcg gccgccgacg gtgtgaacac ctcgtgcacg
      781 atcgggatgt gcaccggatc tccgagcatc atgccgtagt agccgaggtc gcgtagctgc
      841 tcggcccacg cgcggagccc atcgacgtcg tcgcgaccgc caccccacat cccgctcacg
      901 ggatagcgaa tcccggcggc gcgcgcgtcg accagcacct tggaacggag gaacaacgtc
      961 tcccgaccct ccggggtcca gcgatagccg agcgcctgat ggatgtctcc gaatcgggac
     1021 accgctccgc ccaggtacga gacccggggg gacgcgacgc cgatctcgta ggcccgacgg
     1081 agggcctgcg ccgtctcgag gatcggatag atcgcagtgg atccgaccgg gagatcgagc
     1141 tccacctcca tgcagtgcag gagcgcgtgc atcgcatgga tgtcttcggg cccttggacc
     1201 ttcgggacca agactccggc cacggcggtt cccatcacgg cgcgcaagtc cttgagcgtt
     1261 tgccccgacg agggtggttg cacccgagcg aagaggaccg ggcggtcagc gtccggagcc
     1321 aggccgtcga agaaggcgcg catccccgtc cgagcggtgt ggcgcgctgc ctcgggaaac
     1381 ggcgtgcgcg gctcttcgag gtcgaccacg atcgagtcgg caccggagcg caacgcctgg
     1441 tcgacctggt cgacctggtc gccgggcacg aacagcaccg atcgcagtgg ccgagcggtg
     1501 gacttgtcca tcggtcctcc ctggcggatg cttcaggccg gttcggtgcg gagcgcgtcc
     1561 gcggccgcgg tctcgcagac cgtgccggcg aagaacgcgc cgttggctgc cccgtgcagc
     1621 cggacggggt tgaggaacgt gagctctcgg aactgattct cggtcagcag gtcccgctcc
     1681 acgagttcga acgcctcggc gatcggctcg gtcatgtccg ggacgtccca gtgggagatg
     1741 tcggacccca gcactggtcg cagtcgcgcc ccgagcgggt tgatgtcgtc acgaaacgcc
     1801 cacaccagca gcgggtcgtc ggcctcacag ccaaagtaga agtgcggctc gaacaggcgg
     1861 cgcagatcgt cggcctcgtg gatctcacac gccgcgaact cgtcgagaag gtctggacgg
     1921 gccgcgggac gcccgtagta gcgccgcagg tcgtccaggc gtccgaggac gctcggatcg
     1981 ccgtagtcac cgaaatagcg aaggagctcg tcgacgtcga gcaagtccgg gtcgaggccc
     2041 gagatcgcgt caccgttgcg attctcccag tgacccacga gtccc
//
LOCUS       DATCCH010000348         6308 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_496411, whole genome shotgun sequence.
ACCESSION   DATCCH010000348 DATCCH010000000
VERSION     DATCCH010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6308
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496411"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            196..990
                     /locus_tag="VIJ44_06625"
     CDS             196..990
                     /locus_tag="VIJ44_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020549711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5165606.1"
                     /translation="MADEAPRDGLDFTSKVAIVTGGCRGVGRGITERFLAAGAEVVIC
                     CRHEPDTLPAAGGRTAAFVAADVRDPDQIDAVLAFTKERFGRLDVLVNNAGGAPPADT
                     ATVSPKFTTAIVTLNLIAPFVFAQRAFAVMNGQPDGGVIVNISSVSGTRPSPGTAAYG
                     AAKAGLINLTQTLGLEFAPKVRVVCVTVGLVRTEQAHLFYGDEEGVRAVGATVPAGRM
                     AEPGDVGDVCLLLASPLARYVTGEHVVVHGGGEWPAYLQAAKNTRG"
     gene            complement(995..1900)
                     /locus_tag="VIJ44_06630"
     CDS             complement(995..1900)
                     /locus_tag="VIJ44_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165607.1"
                     /translation="MADWLVPLSSHTRFVDRNSRPVTPRFELVRRAALDRRLGTVAAE
                     VRGPLADVQTGDTLWLFWTDHDVGVMAVGRARAPRTRRNRAPELPVVLDQVRTRALVV
                     DPMPSGFIRRWLPDVRSSGIRLDLRPRALEALRAWEKERHDRDEALLRPLGAPTWRAR
                     AGRGGRDRPVDDPVLSSAVPFLRSQDFSVGLTTRDQAPRVVARRARDVLVVHGVGTPG
                     ERTRPDALAAFGRLRGHRWALAHGHDDLDLRVFAWLAFAARPSSDTIAFLDDEGVLVT
                     WLHGAAKVEMGGRSKQRWYQQLGVR"
     gene            complement(1922..2701)
                     /locus_tag="VIJ44_06635"
     CDS             complement(1922..2701)
                     /locus_tag="VIJ44_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012888820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5165608.1"
                     /translation="MTGPPAYPSGHGLLEGRVVVVTAAAGTGIGFATAKRCAEEGATV
                     VLSDHHARRLDEAADTLAAEGRARPLTVVCDVTHDDQVQRLVDTATAEHGGVDVMVNN
                     AGLGGTVDLIDMTDDQWRRVLSVTLDGTFHCTRAALRHMTPRGRGVIVNNASVIGWRA
                     QAGQAHYAAAKAGVMALTRCAAIEAAPHGVRVNAVAPSLAMHPFLSKVTSEELLADLT
                     KREAFGRGAEPWEVANVIVFLASDYASYMTGEVVSVSSQHP"
     gene            complement(2698..3873)
                     /locus_tag="VIJ44_06640"
     CDS             complement(2698..3873)
                     /locus_tag="VIJ44_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY5165609.1"
                     /translation="MELGFSAADEDFRREARDWLTERLSGEFAVVRGRGGPGDEHDLF
                     DERSAWEQALGAAGWIGVGWPREYGGRGLSLTQQVIFYEEYARAQGPGRVGIVGEGLL
                     GPTLIQFATDDQKRRFLPGILAGTEIWSQGYSEPDAGSDLANVQTRADRDGDEWVING
                     QKVWTSLAHWADWCFVLCRTNREAPPHRGISYLLVPMRQPGIEIRPIVQITGTSEFNE
                     VFFDGARTAAVNVVGEVDGGWKVAMGTLAFERGASTLGQQLSFENELTAIIEVAKKQG
                     RGDDPILRQRLADAWIALRIMRFNALRSLTALEQADMTPLTSVHKLYWATFHRRLGEL
                     AVDVLGAGGGVAEDGDELGPLQRLFLYSRADTIYGGSNQIQRNVIGERVLGLAKEPR"
     gene            3915..4370
                     /locus_tag="VIJ44_06645"
     CDS             3915..4370
                     /locus_tag="VIJ44_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEY5165610.1"
                     /translation="MTIEFQTPDALRGAIGTHLGFGDWVEITQERIDLFAEATGDRQW
                     IHVDPERAKQGPFGTTIAHGYLTLSITNQFLPQLLRVPAAKLGVNYGANKIRFPAPVP
                     VGSRIRMGAEIIEVQDVPGGVQVTTRNTVEIDGNDKPACVVEAISRFLF"
     gene            complement(4351..6087)
                     /locus_tag="VIJ44_06650"
     CDS             complement(4351..6087)
                     /locus_tag="VIJ44_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165611.1"
                     /translation="MHGAVDGDRVRERLRDHPWLIVLAAGPAVAEALLVATLGSSGAA
                     ALAPQVTAPTPFDVFHDWRWLLVYHPSWLAFGFEVLAMVAVRSVLIVGLVRAAWPATV
                     DRPAWRDQLRSTVGITVLVALVLLPFAVLTFAMAVVSLSWLFFVSVPVLVIAALLLHR
                     APVSASWWRDRPTWETVRVVLITFLVVTAAGAVLVRVPGPAQMVIAGLVGVASAWCWL
                     RLVQALAGGSPQPRRRPFVVVGLAGVLVLVVGGTAAGFAVSVAVENARTLLAPVAANA
                     SGPPVLIVKGFNSEWDGVTRRWVAGRYRIRRFSYAGLDQHGQPRPYSRAATHRSVRDL
                     ALELRTQVSAFHAATGQPVNVVAESEGSLVALAYLTGSPHAPVGALVALSPILGPGRV
                     YYPTLGDSGWGTAAATVMDGVARALSAVGPVDVSADSPLFRSFVDEGPALRGLLRCPA
                     PGRREFAVIPLDSGVSAPAPVSIGVPHAFVPAFHGGLLGDHTSAQLIGRVLAGRAAPG
                     SGFWAGAGDVVGSVAAAWQAPSLSQGLEPAWRHLPASSDCGAVRVAIRRWLGPEPLTS
                     GTAPGGVRTGSG"
     gene            complement(6115..>6308)
                     /locus_tag="VIJ44_06655"
     CDS             complement(6115..>6308)
                     /locus_tag="VIJ44_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165612.1"
                     /translation="LLNLTTIAAWNQHGFASLRLAIGAGALLALVAAADVAGRRLGLR
                     VRVPTVVDLHALHHDVAAR"
BASE COUNT          993 a         2193 c         2295 g          827 t
ORIGIN      
        1 gtggtcgcgg acgtccgagc ggatcacggc tccgagcgta tcggacacct cattccgctc
       61 accggggtgt cgccacggtc atcacgccgc gaacgcgccc gccgtggaga gtgaaccgcc
      121 cgccgccacc cggcgccggc ggacgatctg ggcgcgtcac cgccggtcct cggttcggtc
      181 cactaccgtc cgggcgtggc cgacgaggca ccgcgcgacg ggctcgactt caccagcaag
      241 gtggcgatcg tcaccggtgg ctgtcgcggt gtcgggcggg ggatcaccga gcggttcctg
      301 gccgccggcg cggaggtcgt gatctgctgt cgtcacgagc cggacaccct gcccgcggcg
      361 gggggacgta cggcagcgtt cgtcgccgcc gacgtgcgcg accccgatca gatcgacgca
      421 gttctcgcgt tcaccaagga gcgcttcggg cgcctcgacg tcctcgtcaa caacgccggg
      481 ggcgcgcccc cggcggacac cgcgacggtg tcgccgaagt tcacgacggc gatcgtgacc
      541 ctgaacctga tcgcgccgtt cgtcttcgcc cagcgcgcgt tcgcagtgat gaacgggcag
      601 cccgacggtg gcgtgatcgt gaacatctcg agcgtcagcg gtacccgacc ctctccgggc
      661 accgccgcgt acggggccgc caaggcgggc ttgatcaact tgacccagac cctggggctg
      721 gagttcgcgc cgaaggtccg cgtggtgtgc gtgacggtcg gcttggtgcg caccgagcag
      781 gcccacctct tctacggcga cgaagagggc gtgcgcgcgg tgggcgccac cgtgcccgcg
      841 ggtcggatgg ccgaacccgg cgacgtcggc gacgtctgtc tcctcctcgc ctcaccgcta
      901 gcccggtacg tgaccggtga gcacgtcgtg gtccacggtg gtggcgagtg gccggcgtac
      961 ctgcaggcgg ccaagaacac ccgcggctga gcgatcagcg aacgccgagc tgctgatacc
     1021 agcgctgctt ggaccgcccg cccatctcga ccttggcggc cccgtgcagc caggtgacga
     1081 ggactccttc gtcgtcgagg aaggcgatgg tgtcgctcga cggtcgagcg gcgaacgcca
     1141 gccaggcgaa cacgcggaga tcgaggtcgt cgtggccgtg cgcgagcgcc caccggtgac
     1201 cacgcagtcg cccaaacgcg gcgagcgcgt cgggtcgggt gcgctcgccc ggcgtgccga
     1261 cgccgtgcac gaccagcacg tcgcgggctc gccgcgcgac gacgcgggga gcctgatccc
     1321 gcgtggtgag accgaccgag aagtcctggg accgcaggaa cgggacggcc gacgagagca
     1381 cgggatcatc gacgggacgg tcacgcccgc cccgcccggc tctcgcccgc caggtcggcg
     1441 cgccgagggg ccgcaggagc gcctcgtcac ggtcgtggcg ttccttctcc caggctcgga
     1501 gggcctccag cgcgcggggc cgcagatcga gccggatgcc gctgctgcgc acgtctggaa
     1561 gccatcgtcg gatgaacccc gatggcatgg gatccacgac gagcgcccgg gtgcggacct
     1621 ggtccaggac gaccgggagc tccggggcgc ggttgcggcg agtgcgaggc gctcgggccc
     1681 gacctacggc catgacgccg acgtcgtggt cagtccagaa caaccagagc gtgtcgccgg
     1741 tctggacgtc ggccagcggt ccgcgcacct cggccgcgac cgttcccagc cgtcggtcga
     1801 gcgccgcacg acggaccagc tcgaagcggg gggtcaccgg gcgggagttg cgatcgacga
     1861 accgggtatg gctcgagagc gggacgagcc agtcggccat cgagacagtg tcgcgggtcg
     1921 cctacgggtg ctggctgctg accgacacaa cctcgccggt catgtaggag gcgtagtcgc
     1981 tggcgaggaa gacgatcacg ttggcgacct cccacggctc ggcgccacga ccgaacgctt
     2041 cgcgtttggt gaggtcggcg aggagctcct cgctggtcac cttggagagg aacgggtgca
     2101 tggcgaggct gggcgcgacg gcattcaccc gcacgccatg cggcgcggct tcgattgccg
     2161 cgcagcgggt gagcgccatc actccggcct tcgccgcggc gtagtgggcc tgaccggcct
     2221 gggcccgcca gccgatcacc gaggcgttgt tcacgatgac cccgcgaccg cggggggtca
     2281 tgtggcgcag cgcggcccgg gtgcagtgga acgtgccgtc gagggtgacg ctgagcaccc
     2341 ggcgccactg gtcgtcggtc atgtcgatga gatcgacggt gccgccgaga ccggcgttgt
     2401 tgaccatcac gtcgaccccg ccgtgctcgg cggtggccgt gtcgacgagc cgttgcacct
     2461 ggtcgtcgtg ggtgacgtcg cacaccacgg tcaagggtcg ggctcgacct tcggcggcga
     2521 gggtgtcggc ggcttcgtcg agccggcggg cgtgatggtc gctgagcacc accgtcgcgc
     2581 cctcctcggc gcagcgcttc gcggtcgcga acccgatccc ggtccccgcg gcggcggtga
     2641 ccacgacgac tcggccctcc aggagcccgt ggccactcgg ataagccgga gggcccgtca
     2701 tcggggctct ttggcgaggc cgaggacgcg ttcaccgatc acgttgcgct gaatctggtt
     2761 cgagccgccg tagatcgtgt ccgcacgcga gtacaggaac aggcgttgga ggggcccgag
     2821 ctcgtcgccg tcctcggcga ctcccccgcc ggcgccgagc acgtcgacgg cgagctcccc
     2881 gaggcgtcgg tgaaacgtcg cccagtacag cttgtggacg ctcgtgagcg gggtcatgtc
     2941 ggcctgctcg agggcggtga gtgagcgcag cgcgttgaac cgcatgatgc gcagcgcgat
     3001 ccatgcgtca gcgagacgtt gccgcaggat cgggtcgtcg ccgcgcccct gcttcttggc
     3061 gacctcgatg atggcggtga gctcgttttc gaagctgagc tgctggccga gggtcgaggc
     3121 gccccgctcg aacgccaggg tgcccatcgc gaccttccag cccccgtcga cctccccgac
     3181 gacgttgacg gccgcggtgc gggcaccgtc gaagaagacc tcgttgaact ccgacgtgcc
     3241 ggtgatctgg acaatcggac ggatctcgat gccgggctga cgcatcggga ccaggaggta
     3301 ggagattccc cggtgcggtg gcgcctcgcg gttggttcga cagagcacga agcaccagtc
     3361 agcccagtgc gccaacgacg tccagacctt ctggccgttg atcacccact cgtcgccgtc
     3421 acgatcggca cgggtctgca cgttggcgag gtccgagccg gcgtcgggtt cggagtagcc
     3481 ctgggaccag atctcggtgc cggccaggat cccgggcagg aagcggcgtt tctggtcgtc
     3541 ggtggcgaac tggatgaggg tcggtccgag cagcccctcc ccgacgatcc cgactcgacc
     3601 ggggccctgg gcccgggcgt actcctcata gaagatgacc tgctgggtga gcgagagacc
     3661 tcgacccccg tactcccggg gccagccgac gccgatccag cccgcggcgc cgagcgcctg
     3721 ctcccacgcc gagcgctcat cgaagaggtc atgctcgtcg ccggggccgc cgcggccgcg
     3781 tacgaccgcg aactcgcccg aaagccgttc cgtgagccag tcccgcgcct cgcgtcggaa
     3841 gtcctcgtcc gcggccgaga agccaagctc cacgcgctcg cagcgtagcg gcgaccggtc
     3901 cgtacgatgc ggtcatgacc atcgagtttc agacccccga cgcgctgcgc ggcgcgatcg
     3961 gaacccacct gggcttcggc gactgggtcg agatcaccca ggagcggatc gacctgttcg
     4021 ccgaggcgac cggagatcgc cagtggatcc acgtcgatcc cgaacgagcc aagcaggggc
     4081 cgttcggcac gacgatcgcg cacgggtatc tcacgctgtc gatcaccaac cagttcttgc
     4141 cgcagctgct gcgagtgccg gcagcgaagc tcggcgtgaa ctacggggcc aacaagatcc
     4201 ggttcccggc gccggttccg gtcgggtcgc gcatccgcat gggggccgag atcatcgagg
     4261 tgcaagacgt tccaggcggc gtgcaggtga cgacccgcaa cacggtcgag atcgacggga
     4321 acgacaagcc ggcctgcgtc gtcgaggcga tcagccgctt cctgttctga cgccacccgg
     4381 tgccgtcccg gacgtcagcg gttccggccc cagccaccgt cggatcgcca ctcgcaccgc
     4441 gccacagtcg ctcgaggctg ggaggtgacg ccaggccggc tcgagccctt gactgagcga
     4501 cggggcttgc caggctgcgg cgacggagcc gaccacgtcg ccggcgccgg cccagaatcc
     4561 ggagccgggc gcggcgcgac ccgcgagcac gcggccgatg agctgggccg aggtgtgatc
     4621 accgagcagg ccaccgtgaa acgccgggac gaacgcgtgt gggacaccga tggacaccgg
     4681 cgccggggcg ctcacccccg agtccaacgg gatgacagcg aactcgcgcc ggccgggggc
     4741 cgggcagcgc agcagtcctc gcagcgcggg gccctcgtcg acgaacgaac gaaacagcgg
     4801 gctgtcagcc gagacgtcga ctggccccac cgcgctgagc gcccgcgcca ccccgtccat
     4861 gaccgtcgcc gcggcggtgc cccatcccga gtcaccgagc gtcgggtaat agacgcggcc
     4921 gggcccgagg atgggactca gcgccacgag cgcaccgacc ggagcatgag ggctgccggt
     4981 gagataggcg agcgcgacca gcgagccttc actttcggcg acgacgttga ccggctgacc
     5041 cgtcgcggcg tggaacgcgc tgacttgggt gcgcagctcg agggccaggt cgcgcaccga
     5101 ccggtgcgtc gcggcgcggc tgtaggggcg gggctggccg tgttgatcca gtccggcgta
     5161 agagaagcgg cggatgcggt accggcccgc gacccaacgg cgggtgacgc cgtcccattc
     5221 cgagttgaat cccttgacga tcagcaccgg cggcccggag gcgttggccg cgaccggtgc
     5281 cagcaacgtg cgggcgttct cgaccgcgac cgagaccgcg aagccggccg cggtgccacc
     5341 gacgaccaag acgagcaccc cggcgagccc cacgacgacg aacggtcgac gccgaggctg
     5401 cggtgatccg ccggccaggg cctgcacgag ccggagccag caccacgcgc tcgcgacacc
     5461 cacgagaccg gcgatcacca tctgggccgg gccgggcacg cgtaccagca ctgcgcccgc
     5521 tgcggtgacc acgaggaagg tgatgagcac gacgcggacg gtctcccagg tgggacggtc
     5581 gcgccaccac gacgcggaga ctggggcacg gtgcagcaac agcgccgcga tgacgagcac
     5641 cggaaccgag acgaagaaca gccacgagag ggacacgacc gccatggcga aggtcagcac
     5701 cgcgaacggg agcagcacaa gcgcgaccag cacggtgatg ccgacggtgg accggagctg
     5761 gtctcgccac gccggtcggt cgaccgtagc cggccaggca gcgcggacga gcccgacgat
     5821 gagcacgctc cgcaccgcga ccatcgcgag cacctcgaac ccgaaggcca gccaggaggg
     5881 gtggtagacg agcagccatc gccagtcgtg aaacacgtcg aagggcgttg gcgcagtgac
     5941 ttgcggtgcc agcgcagccg cgcccgacga tccgagcgtg gccacgagca gcgcttcggc
     6001 gacggctgga cctgccgcga ggacgatcag ccaggggtgg tcgcgcagcc gctcgcggac
     6061 gcggtcaccg tccaccgcgc cgtgcacgcc cgcagcgtac gcggcgcggt cggtctaacg
     6121 ggcggcgacg tcgtggtgca gggcgtgcag atcgacgacg gtcgggacac ggacccgcag
     6181 gccaagccgt cgcccggcga catcggcggc ggcgacgagc gccaggagcg cgccggcccc
     6241 gatggcgagc cgcagactgg cgaagccgtg ctggttccac gcggcgatcg tggtcaggtt
     6301 caagagcg
//
LOCUS       DATCCH010000349         2336 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_288144, whole genome shotgun sequence.
ACCESSION   DATCCH010000349 DATCCH010000000
VERSION     DATCCH010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288144"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="VIJ44_06660"
     CDS             <1..825
                     /locus_tag="VIJ44_06660"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5165613.1"
                     /translation="VTHRRAESDLADRVLALEAGRPVPVPVVAAPPDPLSTGPGPLSA
                     PTGGSVIQLCSVGHVYGRGTPWSTRALEGIDLELESGEGLLVLGHNGSGKSTLAWILA
                     GLLQPSEGTATLDGVPIDHVVGRVGLAFQHARLQLLRSTVANDIAAASGAGLEVVPTA
                     LASVGLDPAIFANRRVDELSGGEIRRAALAGILARHPSVIVLDEPFAGLDDAGRLSLG
                     KLLLRLRHDSGITVVIVSHDDDLPGGLINRVVELERGRVTRDGTVPGLPVAPGGRG"
     gene            822..1670
                     /locus_tag="VIJ44_06665"
     CDS             822..1670
                     /locus_tag="VIJ44_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013828523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEY5165614.1"
                     /translation="MKPVAGFERADSPRGRARRKPELVLLRLLPGTSPLHRLWAGTKL
                     LIVMLLAVLVSVQPTWATLLAVAVVIGTGLIVARVPLGAFPRLPRWFWVLFAVGGLLT
                     LRSGTPPVERVAGLELSLGGVEEWALFAALGIVLIVAALLVAWTTPLGEVAPALARLV
                     TPLRWLRLPVDEWVVAVGLAIRCFPLLLDEIRTLVAVRRLRRRPAPVSRVSFRRAFAH
                     TAREGHDLLTTAIVVALRRARDLAEAIEARGGVTWTPGTKAGPGVADVVAFVIVAAVA
                     AVTLIA"
     gene            1733..>2336
                     /locus_tag="VIJ44_06670"
     CDS             1733..>2336
                     /locus_tag="VIJ44_06670"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEY5165615.1"
                     /translation="MVRDAMAAAVYQGGGRIEVRELPVPSLDPNGVLVEVSHCGICGT
                     DLHLVLEQFARAGSVLGHEWAGTVVATGEAVNGWGPGTRVVWNPQPGCGECRACRRGR
                     PSVCLRRDPPDYLDFRGAFCQYVTVDAGRLLQVPDPLSTRAAALTEPTAIALHTVNLS
                     GVGAEDRVLVTGGGPVGLLTVAVLRTRGVRDITVSEPAPKR"
BASE COUNT          266 a          817 c          829 g          424 t
ORIGIN      
        1 gtgacgcatc gacgcgccga gtcggacctt gccgaccgag tcctcgcgct ggaggccggt
       61 cgtcccgttc cggttcccgt ggtcgcagct ccgcctgacc cgctctccac ggggcccggg
      121 ccgttgtcgg caccgaccgg agggagcgtc attcagctgt gctcggtggg gcacgtctac
      181 ggccggggaa caccctggtc gacccgcgcc ctcgagggga tcgacctgga gctcgaatcc
      241 ggcgagggat tgctggtcct cggacacaac ggttcgggga agtcgacgct ggcgtggatt
      301 ctcgcggggc tgctccaacc gtccgaaggc accgccaccc tcgacggcgt gcccatcgat
      361 cacgtggtcg ggcgagtggg tctggccttc cagcacgccc ggttgcagct gctgcgatca
      421 accgtcgcga atgacatcgc cgcggcgtcg ggcgctggtc tcgaggtcgt cccgaccgcg
      481 ctggcatcgg tcggtctcga ccccgcgatc ttcgccaacc gtcgagtcga cgagttaagc
      541 ggtggcgaga ttcgccgagc ggcgctggcc ggcatcttgg cccgtcatcc cagcgtgatt
      601 gtgctcgacg agccgttcgc gggcctcgac gatgccgggc ggctctcact cggaaagctg
      661 ctgctccggt tgcgtcacga ctcgggcatc accgtcgtga tcgtctcgca cgacgacgac
      721 ctgccgggtg ggctcatcaa ccgcgtggtc gagctcgagc gcggtcgcgt gacacgggat
      781 ggcaccgttc ccggcctccc cgtggcgcct gggggtcgcg gatgaaaccg gtcgctggct
      841 tcgaacgggc cgactccccc cgcggccgtg cgcgccgcaa acccgagttg gtcctcctcc
      901 ggttgctccc gggaacgtct ccgctgcatc ggctgtgggc gggaacgaag ctgctgatcg
      961 tcatgctgct cgcggtgctg gtgtcggtgc agccgacgtg ggcgaccctg ttggccgtcg
     1021 cggtcgtgat cggcacgggt ctcatcgtcg ctcgggtccc gctcggggcg ttcccgcggc
     1081 tgccgcgatg gttctgggtc ctgttcgccg tcggtgggct cctcaccctc cggtcgggta
     1141 cgcctcccgt cgaacgggtc gccgggctcg agctcagtct cggcggcgtc gaggaatggg
     1201 cgcttttcgc ggcattgggg atcgtgctca tcgtggcggc gttgctcgtc gcttggacga
     1261 caccgctggg tgaggtggcc ccggccctgg ctcgactcgt cacaccgttg cgatggctcc
     1321 gcctcccggt cgacgagtgg gtggtcgcgg tgggtctggc catccgctgc tttccgctcc
     1381 tgctcgacga gatccgcacc ctggttgcgg tccggcgact ccgacgtcgc cccgctccgg
     1441 tctctcgcgt ctcgtttcgg cgcgccttcg ctcacacggc gcgggaggga catgacctct
     1501 tgaccaccgc catcgtggtc gccttgcgtc gggcccggga cctcgccgag gcaatcgagg
     1561 cccgcggtgg tgtcacgtgg acacctggaa ccaaggccgg acccggggtg gccgatgtgg
     1621 tggcgttcgt catcgtcgcg gccgtcgcgg ccgtcacctt gatcgcttga cctccagccg
     1681 cggtcacggt cggtaggctc cgcccgagcc cttggtcata acatcgcggc ctatggtccg
     1741 cgacgcgatg gccgccgcgg tctaccaggg cggcgggcgt atcgaggtgc gggagctccc
     1801 cgtcccgtcg ctggatccga acggggtcct ggtggaggtc tcgcattgcg ggatctgcgg
     1861 caccgatctg cacctcgtgc tcgaacagtt cgcccgggcc ggttcggtgc tcggccacga
     1921 gtgggccggc accgtcgtcg cgaccggcga ggccgtcaac ggctggggac ccggcacccg
     1981 tgtggtctgg aacccccaac ccggctgtgg ggagtgtcgg gcctgtcggc ggggccgacc
     2041 ttcggtgtgt ctacggcgcg acccgcctga ctatctcgac tttcggggag cgttctgcca
     2101 gtacgtcacg gtcgacgccg gccggctcct ccaagtcccg gacccgttgt cgacgcgcgc
     2161 cgcggcactc accgagccga ccgcgatcgc gttgcatacc gtcaacctct cgggtgtcgg
     2221 ggccgaggat cgcgtcctcg tcaccggtgg cggaccggtc ggcctgctga ccgtggcggt
     2281 cttgcggacc cgcggggtcc gcgacatcac cgtgtccgag ccggctccga agcggc
//
LOCUS       DATCCH010000350         3457 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_329175, whole genome shotgun sequence.
ACCESSION   DATCCH010000350 DATCCH010000000
VERSION     DATCCH010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329175"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="VIJ44_06675"
     CDS             <1..721
                     /locus_tag="VIJ44_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007456587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEY5165616.1"
                     /translation="PDQTLDRGAVAGRLSELDEYAVEQAVRLVEGGIDAEITYLTVGP
                     QTAKDGLLKALAIGGDRGVHVLDDAIHGSDSLATSLVLAKALEHLGFDLVLAGMSSTD
                     AAMSVVPSMVAERLGVSQATFAGSLSVDGTTVSITRESDTATEHVRVELPAVVSITDQ
                     TDEARYPAFRAIMMAKKKPIETLSLADIGVDPRAVGLAAAATRVVAVSSAPAKAAGTV
                     VVDDGTGAAQLADFLVARGAL"
     gene            850..1812
                     /locus_tag="VIJ44_06680"
     CDS             850..1812
                     /locus_tag="VIJ44_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEY5165617.1"
                     /translation="MSEILVLAELTPTGVRKASLELLTIARRLGEPSAVVCGDATEDV
                     VARLGAYGATTVYAVSAPEIEQFLSLPKVEVVVTLAERVRPAAILIASGPEGKDVAAR
                     VAVRLDSGLITDAVDVTAEGATITTTQSVFAGAFHVRSEVVRGTPVITVMPNAVTAEA
                     APAQPRVESVDVAFSDRAKGAVVTSRTPKGSSARPDLADAAVIVAGGLGVGSEAGLGV
                     IEQLADALGGAVGVTRAVSDLHWAPHDLQIGQTGKTVAPGLYVAAGVSGAIQHRAGMQ
                     SSKTIVAVNKDPKAPIFSIADFGVVGDLHTVLPALVDEISKRRT"
     gene            1858..2514
                     /locus_tag="VIJ44_06685"
     CDS             1858..2514
                     /locus_tag="VIJ44_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165618.1"
                     /translation="MATFKLLLGLGMTVVLLGIAGPRLGFLCRVGASAQPVEPGRVGR
                     TGDIARAEIVEVAAQKKLLRWTAPGLAHFAVFWGFLVLVLTIIEGYGALFSPTFHIPV
                     IGMWPALGFFEDLFAVICLLAVAAFTVIRLRESPRKHGINLNRTDEALALSPDLVTAA
                     CPFCVTMLTDGVAQRRMEGAADDSVEVLDIAEVLLRSVRPDLDDASSSGATTAPTSEQ
                     "
     gene            2523..>3457
                     /locus_tag="VIJ44_06690"
     CDS             2523..>3457
                     /locus_tag="VIJ44_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY5165619.1"
                     /translation="MTDTVTTTAEPAGHFGGAGLDLGSLNLMLEALTDFVDAALSPDL
                     QLQLDHEDRCPEDIVRAMSDPMTLGVQLVFIPEQYGGMDGDAFDSYRVCEVLARKDIG
                     LATAVFATFLGSDPIVVGATEEQKQEWLGAIAEQGVVFAYGATEPDAGSDLGAMTTTA
                     TPVTDDDGTVTAYRLNGRKQWISNGSIADYSTILAATPEGPTWFVVAKDTPGFTVGTP
                     EDKHGLRLSNTASLFLDDVEVPAGNLVGTHGGLGLVQAQQVFGYTRLMVAAFGLGGGW
                     EALDRAIAYSVQREQGGGPLSEKQGFTHKLIVPHAV"
BASE COUNT          508 a         1332 c         1112 g          505 t
ORIGIN      
        1 gcctgaccag acgctcgacc gcggcgctgt cgcgggacgg ctctccgaac tcgacgagta
       61 cgccgtcgaa caggccgtgc gcctcgtcga gggtgggatc gacgccgaga tcacatacct
      121 caccgtcgga ccccagaccg cgaaggacgg actgctcaag gcgctcgcga tcggcggcga
      181 caggggtgtc cacgtcctgg acgacgccat ccacgggtcg gactcccttg ccacctcgct
      241 ggtgctcgcc aaggcgctcg agcacctcgg attcgacctc gtcctcgccg ggatgtcgtc
      301 gaccgacgcc gcgatgtcgg tcgtcccctc gatggtcgcc gaacgcctcg gcgtcagcca
      361 ggccactttc gctgggtcgt tgtccgtgga cggaacgaca gtttcgatca cccgagagag
      421 cgacaccgcg accgagcacg tgcgcgtcga actgcctgca gtcgtgtcga tcaccgacca
      481 gaccgacgag gcccgttacc ccgccttccg ggcgatcatg atggcgaaga agaagccgat
      541 cgagaccctc tcgctcgcgg acatcggggt cgacccccgt gcggtcggac tcgcggcggc
      601 cgccacacgc gtcgtcgccg tgtcctccgc gcccgccaag gccgcgggca ccgtcgtcgt
      661 cgacgacggc accggcgcgg ctcagctcgc cgacttcctc gtcgcccgcg gcgccctgtg
      721 acgatgagcc gcccgcgacg ccctgtaacg atgagccgga ccgcctcggc gccagcacct
      781 cgtccagcac cgtggtgcgt cgcgtccacc gcatccacct tcgtgcccga ccactggtag
      841 gagaccaccg tgtccgagat cctcgtcctc gccgagctca ccccgaccgg agtccgcaag
      901 gccagcctcg agctgctcac catcgcccgc cgcctcggcg agcccagcgc ggtcgtctgc
      961 ggcgacgcca ccgaggacgt ggtcgcgcga ctcggcgcgt acggcgccac caccgtgtat
     1021 gccgtgtccg caccggagat cgagcagttc ctctccctcc ccaaggtcga ggtcgtcgtc
     1081 acgctcgccg agcgggtccg gcccgccgcc atcctcattg cctcaggccc ggagggcaag
     1141 gacgttgccg cgcgggtcgc cgtccgcctc gatagcggcc tgatcaccga cgccgtcgac
     1201 gtgaccgccg agggcgccac gatcacgacg acccagtcgg tcttcgccgg agccttccac
     1261 gtccgctccg aggtcgtgcg cggcaccccg gtgatcaccg tcatgcccaa cgccgtcacc
     1321 gccgaggccg cgccggcgca gccgagggtc gagagcgtcg acgtcgcgtt cagcgaccgg
     1381 gccaaggggg ccgtggtgac cagccgcacc ccgaagggct cgagcgcccg tcccgacctc
     1441 gcggacgcgg ccgtcatcgt ggctggtggc ctcggggtcg gctccgaagc cggcctgggc
     1501 gtcatcgagc agctcgctga cgccctcggt ggagccgtgg gggtcacccg tgcggtgagc
     1561 gacctgcact gggcccccca cgacctgcag atcgggcaga ccggcaagac cgtcgcccca
     1621 ggcctgtacg tcgccgccgg cgtctccggg gcgatccagc accgcgccgg catgcagtcg
     1681 agcaagacga tcgtcgcggt gaacaaggac ccgaaggccc ccatcttctc gatcgccgac
     1741 ttcggggtcg tcggcgacct gcacaccgtc ctccccgccc tcgtcgacga gatcagcaaa
     1801 cgccggacct gactccgcgc ccccgacggg cgcaccacac gcaaaggagc gtgaccgatg
     1861 gcaaccttca agctgcttct gggcttgggc atgacggtcg tgctgctcgg gatcgccggc
     1921 cctcgcctgg ggttcctctg ccgcgtcggc gcctccgccc agccggtgga gccgggtcgc
     1981 gtcggcagga ccggtgacat cgcccgcgcc gagatcgtcg aggtggcggc acagaagaag
     2041 ctgttgaggt ggaccgcccc cgggctggca cacttcgccg tcttctgggg tttcctggtc
     2101 ctcgtcctga ccatcatcga ggggtatggg gcgctgttct cgccgacgtt ccacatcccc
     2161 gtcatcggga tgtggcccgc cctcggcttc ttcgaggacc tgttcgccgt catctgcctg
     2221 ctcgccgtcg ccgcgttcac ggtgatccgg ctccgcgagt cgcccaggaa gcacgggatc
     2281 aacctcaacc gcaccgacga ggccctcgcg ctctcccccg acctggtcac cgcggcctgc
     2341 ccgttctgcg tgaccatgct caccgacggc gtcgcgcagc gccggatgga gggcgcggcg
     2401 gacgactcgg tcgaggtgct cgacatcgcc gaggtgctcc tgcgctcggt tcgacccgac
     2461 ctcgacgacg cctcgtcgtc cggcgccacc accgcaccca cgtccgaaca gtgaggacca
     2521 ccatgaccga caccgtcacg accaccgcag agccggccgg ccacttcggc ggagccgggc
     2581 tcgatctggg gagcctgaac cttatgctcg aggcgctgac cgacttcgtc gacgccgcgc
     2641 tctcacccga cctccagctg cagctcgacc acgaggaccg ctgccccgag gacatcgtgc
     2701 gggccatgtc cgacccgatg accctcggcg tccagctcgt cttcatcccc gagcagtacg
     2761 gcgggatgga cggcgacgcc ttcgactcct accgggtctg cgaggtcctg gcccgcaagg
     2821 acatcggtct cgcgacggcc gtgttcgcga ccttcctcgg ctcggacccc atcgtcgtcg
     2881 gggcgaccga ggagcagaag caggagtggc tcggggccat cgccgagcag ggcgtcgtct
     2941 tcgcctacgg cgccaccgag ccggacgccg gctccgacct cggcgccatg acgacgacgg
     3001 ccaccccggt caccgacgac gacggcacgg tcacggccta ccggctcaac ggccgcaagc
     3061 agtggatctc caacggctcc atcgccgact actccaccat cctcgccgcg acccccgagg
     3121 gcccgacgtg gttcgtcgtc gccaaggaca cccccgggtt cactgtcggc acccccgaag
     3181 acaagcacgg gctacggctg tccaacaccg cgtcgctctt cctcgacgat gtcgaggtcc
     3241 ccgctggcaa cctcgtcggc acccacggcg gcctcggact ggtccaggcc cagcaggtct
     3301 tcgggtacac ccggctcatg gtcgccgcat tcggcctggg gggcggctgg gaggccctcg
     3361 accgtgccat cgcctactcg gtgcagcgcg agcagggtgg cggcccgctc agcgagaagc
     3421 agggcttcac gcacaagctc atcgtcccgc acgccgt
//
LOCUS       DATCCH010000351         2395 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_288457, whole genome shotgun sequence.
ACCESSION   DATCCH010000351 DATCCH010000000
VERSION     DATCCH010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288457"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            220..882
                     /locus_tag="VIJ44_06695"
     CDS             220..882
                     /locus_tag="VIJ44_06695"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HEY5165620.1"
                     /translation="MTLPRTEVEPGILAELASFHELLESLDEQAWETPSRCAGWTVAN
                     VASHVVGSMADVVGGRLEGLGSPEATAREVGEREGRTPAELAQELTDVTKQATDLLAV
                     FDDAAWNATAPGGYTGTLGEGIEALWYDTWAHHDDICAALRQPPAPGPGLRAGLHHVA
                     ENLEVQEWGPATLALDGVEEIPIGAGGAKVTGDPYAFLLAATGRGDPAAFGLDETVNI
                     YR"
     gene            complement(1199..>2395)
                     /locus_tag="VIJ44_06700"
     CDS             complement(1199..>2395)
                     /locus_tag="VIJ44_06700"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY5165621.1"
                     /translation="RRPEPATAMFPAGSYSYAAGPSRRGAARSRRRHRRTRWALAAAA
                     ASVAIAAGGIWAQSATRTAQATGRTAAATPVRNKDLSAAAIADQSDAAIVDVVTTLGY
                     QNGQAAGTGIVLTSSGEILTNHHVVQGSTSITVKVSNSSQTYKARVVGFDATDDIAVL
                     QAEGVSGLTTAPLGDSSAVAVNQSVVAIGNALNKAGLPTVTEGAVTQLGRSISVSGDN
                     GTVEQLKNLLETSAPLQPGNSGGPLFDTSGQVIGINTAASAGNIPDGGTTDGYAIPIN
                     DALTVAHQIESGHNTDTVHIGASPFLGVSLRGGGQGFGRSAGSSSGSGVLVAGVEPGT
                     PAEAAGITAGDQIIAVSGTTVSSQSELTAALSAKHPGDTITVTWVDQSDAQHQSTIRL
                     ATGPIA"
BASE COUNT          334 a          876 c          849 g          336 t
ORIGIN      
        1 ctgacgcacg atgtcctccc gggccgtgcc ggcgaaaccc cgcgctgcga acagcgcccg
       61 acccgaatcg agcagggccc ggcgggtcgc cgccgagcgc tcgggctgcc gccggcggca
      121 gcgggtccgt cgccgtgacc caggtcaccg ttgactgcca tcaccgaagg gcgtagagat
      181 gcgtgcagcc tgcacgtaac gtcgaaaggg agacacccga tgacgctgcc gcgcaccgag
      241 gtcgaacccg ggatcctggc ggagctcgcc tcgttccatg agctcctcga gagcctcgac
      301 gaacaggcct gggagacccc cagccgctgt gcgggctgga cggtcgccaa cgtggcgagc
      361 cacgtcgtcg gctcgatggc cgacgtcgtc ggcgggcgcc tggaggggct gggctcaccg
      421 gaagccaccg cacgcgaggt cggcgaacgc gaaggccgca ccccggcgga actcgcccag
      481 gagctgaccg acgtcaccaa gcaggccacc gacctcctcg cggtgttcga cgacgcggcg
      541 tggaacgcca ccgcgcccgg cggctacacc ggaacgctcg gcgaagggat cgaagcgctc
      601 tggtacgaca cctgggccca ccatgacgac atctgcgccg ccctccgaca gccgccggcc
      661 cccggcccgg gtctgcgcgc cgggctccat cacgtcgccg agaacctcga agtgcaggaa
      721 tggggcccgg ccacactcgc cctcgacggc gtcgaggaga tcccgatcgg cgccggcggc
      781 gccaaggtga ccggcgaccc gtacgccttc ctgctcgccg ccaccggccg cggtgacccc
      841 gcagcgttcg ggctcgacga aaccgtcaac atctaccggt agtcgccgcc gcctccctcc
      901 gccgttctca tgaggctccc ccgccatgcc aggcggggca tctccgaacg atggccaagc
      961 cgcgcctccc ggctggacga ggccgagcca gtgggccccg cagatgccgg ccggtccgcc
     1021 tcgccgcccg cctcccccgc gacggccccc gccgactccg ccccgtgtcg cgggtccacc
     1081 ccgcggtcag accagcgacc agccgagcgc cccatgcgtc gacgtcgaag gtatgcgtgg
     1141 cggcctactc gggcgccatc ctcgtcttcg aacgacgcgt agcgcggcga gtgcccgatc
     1201 aggcgatcgg cccggtagcg agccggatcg ttgactggtg ctgggcgtcc gactggtcga
     1261 cccacgtgac ggtgatcgtg tcgcctgggt gcttggcgct gagcgccgcg gtgagctcgc
     1321 tctgggagga gaccgtcgtc ccggagacgg cgatgatctg atcgcccgcg gtgatgcccg
     1381 ccgcctcggc gggggtgcca ggctcgacgc cggcgacgag caccccggag ccactcgagc
     1441 tgccggcgct acggccgaag ccttggccgc cgccgcggag cgagacgccg aggaacgggc
     1501 tcgccccgat gtgcacggtg tcggtgttgt gcccagattc gatctggtgc gcgacggtga
     1561 gcgcgtcgtt gatcgggatc gcgtagccgt cggtcgtgcc cccgtccggg atgttgccgg
     1621 cggacgcggc ggtgttgatc ccgatgacct gccccgaggt gtcaaagagc gggccaccgg
     1681 agttgccggg ctggagcggg gcgctggtct cgagcaggtt cttgagctgc tcgaccgtcc
     1741 cgttgtcgcc actgaccgag atcgaacgac ccaactgcgt gacggcccct tcggtcacgg
     1801 tcgggagacc ggccttgttc aaggcgttgc cgatcgcgac gacagactgg ttgaccgcga
     1861 ccgccgatga gtccccaagc ggcgcggtcg tgagcccgga gaccccttcg gcctggagaa
     1921 ccgcgatgtc gtcggtggcg tcgaagccga ccaccctcgc cttgtaggtc tgcgagctgt
     1981 tcgagacctt caccgtgatc gacgtcgaac cttggaccac atggtggttg gtcaggatct
     2041 ctccgctcga cgtgagcacg atcccggtcc ctgcggcctg gccgttctgg tagccgaggg
     2101 tggtgacgac gtcgacgatt gcggcgtcgc tctggtcggc gatggccgcg gccgagagat
     2161 ccttgttccg gaccggcgtt gccgccgcgg tgcgcccggt cgcctgggcg gttctggttg
     2221 cgctctgcgc ccagatcccg ccggcagcga tcgcgaccga ggcggccgcc gcggcgagcg
     2281 cccaccgggt tcggcggtgc cgacgccgcg accgcgccgc gccccgccgc gacgggcccg
     2341 ctgcgtacga gtacgacccg gccgggaaca tcgcagtggc cggttccggg cggcg
//
LOCUS       DATCCH010000352         2799 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_496945, whole genome shotgun sequence.
ACCESSION   DATCCH010000352 DATCCH010000000
VERSION     DATCCH010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496945"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="VIJ44_06705"
     CDS             complement(<1..522)
                     /locus_tag="VIJ44_06705"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEY5165622.1"
                     /translation="MTTRPAYRDLPDGCAWEYLDAELGSLERLTADRVVDAARLVRRG
                     ARFSLDLPLDVPSPPLFGREAMRHEIFELFDQVLDDRLDNFFPQASTQWDGFGHFGHS
                     ARGFFGSRTAAQVRGDRGVLAWTEHGIVGRGVLLDVARRVDIPGDEPFVVTPELLDET
                     VAEQGVEVRDGDVC"
     gene            complement(519..1700)
                     /locus_tag="VIJ44_06710"
     CDS             complement(519..1700)
                     /locus_tag="VIJ44_06710"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEY5165623.1"
                     /translation="MSPSLHFAPVDFDDFHQVELPARIAAGHGGLAARDVAGVEPIAF
                     QLADGPAYTYRPAGDTVTIVAGVASARTVVELSPDAWSDYVNELRTCFGLLYANLVSF
                     PQGGFDDLARWEPALRALYHGRPIYDPDAVTLPPDLGRAFTLDDANWPTFFKQTGFVH
                     LRHVFEPSEVLPVSDEVERLVAAARPGDGRSWWATRADGAEVCCRVTYTTLASPTIAA
                     LGDDSRVRRLAELGPPGLVPTLDCLDGFAAVLKQPDVVEGLSDLPWHQDCGLGGHPVI
                     CPGIQIGIQLDAATAGTGQLHFLAGSQRSSCHPIRPGDQASPVVAVDTEPGDVTVHDP
                     HVLHAAPPPTGSGSGRRALYASFVRPETTAYVGAGRAYNDVLFEHDTRVRSVEEVRSG
                     P"
     gene            complement(1697..2692)
                     /locus_tag="VIJ44_06715"
     CDS             complement(1697..2692)
                     /locus_tag="VIJ44_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014176079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY5165624.1"
                     /translation="MAMLGVRFDLRVPDFAPTTHARQYDAALDMAGWADRVGLDMVIL
                     SEHHGAPDGYLPAPLTLAAAVLGRTPRIPVSVAAILVPLHDPIRLAEQLAVLDLCAPG
                     RLSVVAGVGYRSVEFAMAGVERADRGRLLEDGIEVLRQAWSGEPFTWRGRTVVVTPRP
                     ATPGGPIVMIGGSTEIAARRAARLHCGFFPALGDPHLADVYQEECTAVGFDTGFCSMP
                     SGPGFVCVAEDPEAMWARIGPHALFDAQTYAAWQEGPQRSAVDVKGAQTWQDVRDTGV
                     YQVLTPDECVDLAHQQGDFGSVLLHPLMGGLPPELGWESLELFERQVLPRLRPSP"
BASE COUNT          442 a          916 c         1064 g          377 t
ORIGIN      
        1 gcagacgtca ccgtcgcgga cctccacccc ctgctccgcc acggtctcgt cgaggagctc
       61 gggagtcacc acgaacggct catcgccggg gatgtcgact ctgcgggcga cgtcgagcag
      121 gaccccgcgc ccgacgattc catgctcggt ccacgccagg acgccacggt cgccgcggac
      181 ctgcgccgcg gtccgggagc cgaagaaccc tcgggcggag tgaccgaagt gcccgaagcc
      241 gtcccactgg gtcgaggctt gcgggaagaa gttgtcgagc cggtcgtcga ggacctgatc
      301 gaacagttcg aagatctcgt ggcgcatcgc ctcgcggccg aacaacgggg gcgacgggac
      361 atcaagcggg agatcgagac tgaagcgggc accgcgccgt accagccgcg ccgcgtcgac
      421 gacccggtcc gcggtgagcc gctcgagcga gccgagctcg gcgtcgaggt actcccacgc
      481 gcacccgtcg ggaagatcgc ggtacgcggg cctggtggtc acggccccga tcggacttct
      541 tcgacggagc gaacccgggt gtcatgttcg aacaagacat cgttgtacgc gcgaccggcg
      601 ccgacatacg ccgtcgtctc cggtcggacg aagctggcgt atagggcccg ccgtcccgat
      661 ccggatccgg tcggcggagg tgccgcgtgc agcacgtgcg ggtcgtggac ggtcacgtct
      721 cccggctcgg tgtcgaccgc gacgaccggg ctggcctggt caccggggcg gatcgggtga
      781 caggagcttc gctgcgagcc ggccaggaag tggagttggc cggtgccggc ggtcgcggcg
      841 tcgagctgga tcccgatctg gattccgggg cagatcaccg ggtgtccgcc cagcccgcag
      901 tcttgatgcc acggcaggtc ggacaggcct tccaccacgt cgggctgctt cagcaccgcg
      961 gcgaacccgt ccaggcagtc gagggtcggg acgagaccgg gcgggcccaa ctcggcgagg
     1021 cggcggaccc gggagtcgtc acccaacgcg gcgatggtcg gcgaggccag cgtcgtatag
     1081 gtgacgcggc agcacacctc ggcaccgtcg gcgcgcgtgg cccaccagga gcggccgtcg
     1141 ccgggacggg ccgcggcgac aagacgttcg acctcgtcgg agaccgggag aacctcggag
     1201 ggctcgaaca cgtggcggag gtgcacgaag ccggtctgct tgaagaacgt gggccagttg
     1261 gcgtcgtcga gagtgaaggc gcgccccaga tccggtggga gcgtcaccgc gtcggggtca
     1321 tagatcggcc ggccgtggta cagggcccgc aatgcgggct cccagcgggc caagtcgtcg
     1381 aacccgccct gggggaacga caccagattg gcgtagagga gaccgaagca ggtgcggagc
     1441 tcgttcacgt agtcggacca cgcgtccggg ctcagctcga ccacagtgcg ggccgacgcc
     1501 acgccggcca cgatcgtcac cgtgtcgccc gcgggacggt aggtgtaggc aggcccgtcg
     1561 gcgagctgga aggcgatcgg ctcgacgccg gcgacgtcgc gagcggccag gccaccgtga
     1621 ccggcggcga tgcgcgccgg caactcgact tggtggaagt catcgaagtc gaccggcgcg
     1681 aagtgcaagc tgggactcac ggcgagggtc gcagccgcgg caggacctgg cgctcgaaga
     1741 gctcgagcga ttcccagccg agctcgggtg ggaggccacc catcaacggg tggaggagca
     1801 cggagccaaa gtcgccttgc tggtgtgcga ggtcgacgca ctcgtcgggg gtgaggacct
     1861 ggtagacgcc ggtgtcgcgc acgtcttgcc aggtttgcgc ccccttgacg tcgacagcgg
     1921 accgttgcgg tccttcttgc cacgcggcgt aggtctgggc gtcgaagaga gcgtgcgggc
     1981 cgatccgagc ccacatcgcc tcggggtctt cggcgacgca cacgaagccg ggcccggacg
     2041 gcatcgagca gaacccggta tcgaagccga cggcggtgca ttcttcttgg tagacgtcgg
     2101 cgagatgcgg gtcgccgagc gcggggaaga atccacagtg caggcgtgca gcgcgccgtg
     2161 cggcgatctc ggttgagccg ccgatcatga cgatggggcc accgggggtc gcgggcctcg
     2221 gggtgaccac cacggttcgg ccccgccatg tgaacggctc gccgctccag gcctgtcgca
     2281 gcacctcgat gccgtcctcg aggaggcggc cccgatccgc gcgctcgacg cccgccatcg
     2341 cgaactcgac gcttcggtac ccaacgccgg cgacgacgct gagccggccg ggagcgcaga
     2401 ggtcgaggac cgcgagctgt tcggcgaggc ggatcggatc gtggagcggg acgaggatcg
     2461 cggcgacgct gaccgggatg cggggcgtgc gacccaagac cgcggcggcg agggtgagcg
     2521 gcgccgggag atagccgtcg ggtgcgccgt ggtgctccga gaggatgacc atgtcgaggc
     2581 cgacccggtc ggcccatccg gccatgtcca gtgcggcgtc gtattggcgg gcgtgggtcg
     2641 tgggcgcgaa gtcggggacc cgcaggtcga agcgcacgcc gagcatcgcc attggtcccc
     2701 cctctgttcg tcgacgtccg tcgcggcccg gatgataggg cggagcgggt cgggccttct
     2761 ggggatcatg gccatgacgc ccggtgcggt cccggccga
//
LOCUS       DATCCH010000353         2440 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_329804, whole genome shotgun sequence.
ACCESSION   DATCCH010000353 DATCCH010000000
VERSION     DATCCH010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329804"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..530)
                     /locus_tag="VIJ44_06720"
     CDS             complement(111..530)
                     /locus_tag="VIJ44_06720"
                     /inference="COORDINATES: protein motif:HMM:NF023430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3499 family protein"
                     /protein_id="HEY5165625.1"
                     /translation="MARLCARPGCSAPAAATFNFDGLNRIVWLNAFTNAAAYSAGDLC
                     ARHAERLRPPRNWELRDCRPSPAARQPGATAPSRHYAPDPEPRRDRVPTAAGPVGVER
                     PAASVPLVSHERETVRPQVASARTPLLARAFRGAGGD"
     gene            818..2410
                     /locus_tag="VIJ44_06725"
     CDS             818..2410
                     /locus_tag="VIJ44_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY5165626.1"
                     /translation="MSVAAVDGRVGLPCPVAELRAQHWDVIVVGAGHNGLTAAAYLAR
                     AGCSVLVLERREQIGGACTIERPFADDRFVVSPCAYLVGLLDPRVLKELDLAGHGLRT
                     IAIDPTQWTPFEDGTSLYQWQDPIETARSVAAISPGDVDGFLAYEALFDRIRDGLRSG
                     PLGDTWEGDAPARADVESIFADDAEAREVLLDASIADVVERHVRDERLRTAMHGQGVI
                     GTFAGPRDRGTAWVHAHHRLGLLGGWKFVAGGMGQVSFALADAARAAGAVVAAGTPVV
                     AIEPGEGVRLDGGDRIRAAAIVANVDPKRLLALVDDTDVPPSFAGRVAAWQSTSPVVK
                     LNCALSRLPTFPAAAGDKTCYRGQVEIGRSIDDTQAASVAARRGAAAPEWCELYFQTV
                     YDPSVAAPGCHTMSVFAQYAPYELAAGSWALHRERIADATVETIARFAPDVARCIIGR
                     QVLGPPDVEARIGLTGGHIFQGEILPSQMWERRFAARTPIDGVYLCGAATHPGGSVMG
                     INGRNAAASVLRDQGRSLEGAR"
BASE COUNT          338 a          831 c          909 g          362 t
ORIGIN      
        1 ggcgcgacgt cgacggaacc ggcggagccg gctccgggtc tccgcgtcgc ggaaggtggt
       61 gcgccacccg cgggtgagcg gagtgttggg cagctcaggc ggcggtgcgg tcaatcgcct
      121 ccggcgcctc gaaaggcccg agcgagcagc ggtgtcctcg ccgacgcgac ttggggccgc
      181 acggtttctc gttcatgaga gacgagcggg accgacgcgg ccggccgttc caccccgacg
      241 ggtcccgccg cggttgggac ccgatcacgg cgcggctcgg ggtccggcgc atagtggcgg
      301 ctcggcgccg tcgcgccggg ttgccgcgcc gcgggcgacg gtcgacagtc gcgcagctcc
      361 cagttccggg gcgggcgaag ccgctcggcg tgacgggcgc acaggtcgcc cgcgctgtag
      421 gcggccgcgt tggtgaacgc gttgagccag acgatccggt tcaaaccgtc gaagttgaac
      481 gtcgccgcgg caggcgcgct gcacccaggc cgcgcacaca aacgggccat gaactgaaaa
      541 taccggcttc ctcctcccgg cccgcggatg gtcgcgtcag gcgggtgtcg tccgcgagca
      601 aggcgacgag accggggttg tcgcgttcga tcgagcccga cgagggctcc ccgcggggag
      661 cgctcacggg ctgagtgtac ggtcgccctg atgggcgaag tgtgggtgcc accggtgggc
      721 gccgtgctcg gtgcccgtgg cgacccgccc aacccgtgct tcgcgtctcg atcatgagcg
      781 agcttcccgg tcggtgccga agcgttcacc ccgacggatg agcgtcgcgg ccgtcgatgg
      841 acgggtgggt ctgccgtgtc ccgtcgccga gcttcgggcc cagcactggg acgtgatcgt
      901 cgtcggcgcc gggcacaacg ggctcaccgc ggcggcctac ctcgcgcgcg cgggatgctc
      961 ggtgctggtc ctggaacgcc gggagcagat cgggggggca tgcacgatcg agcgcccgtt
     1021 cgcggacgac cggttcgtcg tgagcccgtg cgcgtacctg gtcggcttgc tcgatccccg
     1081 ggtgctgaag gagctcgacc tggccggaca cggcctccgc acgatcgcga tcgacccgac
     1141 gcagtggaca ccattcgagg acggcacgtc cctctaccag tggcaggacc cgatcgagac
     1201 ggcgcggtcc gtcgcggcca tcagccctgg cgacgtcgac ggcttcctgg cgtacgaggc
     1261 gctcttcgac cgcatccgag acggtctgcg gagcggtccg ctgggtgaca cctgggaggg
     1321 cgatgccccg gcccgggcgg acgtcgaatc gatcttcgcc gacgacgccg aagcccgcga
     1381 ggtgctcctt gatgcctcga tcgccgacgt cgtcgaacgt cacgtccgcg acgaacggct
     1441 ccggaccgcc atgcacggcc aaggtgtgat cggcacgttc gcgggaccgc gtgaccgggg
     1501 taccgcctgg gtccatgccc accaccgcct ggggctgctc ggagggtgga agttcgtcgc
     1561 cggcggcatg ggccaggtgt cgttcgcgct cgccgacgcg gcccgggctg ccggagcggt
     1621 cgtggctgcc gggaccccgg tggtggccat cgaaccgggt gaaggggttc gactcgacgg
     1681 gggagatcgg atccgtgcgg ccgcgatcgt ggccaacgtc gacccgaagc ggctgttggc
     1741 gttggttgac gacaccgacg tgccaccttc gttcgccggt cgcgtcgcgg cttggcagag
     1801 caccagcccg gtggtcaagc tcaactgtgc cttgtctcgg cttccgacgt tcccggccgc
     1861 ggccggtgac aagacctgct accggggaca ggtcgagatc ggtcgcagca tcgacgacac
     1921 ccaggccgcg tcggtcgcgg cgcggcgagg agctgcggcc cccgagtggt gtgagctgta
     1981 cttccagacg gtgtacgacc cgagcgtggc ggcaccgggc tgtcacacca tgagcgtgtt
     2041 cgcccagtac gcgccctacg agttggcggc cggcagctgg gccctccacc gcgagcggat
     2101 cgcggacgcc accgtcgaga cgatcgcccg gttcgcgccg gatgtcgccc gttgcatcat
     2161 cggacgccag gtcctcggac ctcccgatgt cgaagcccgg atcggtctga ccggcgggca
     2221 catcttccaa ggtgaaatcc tcccttccca gatgtgggaa cgacgctttg ccgcccggac
     2281 cccgattgac ggcgtgtacc tgtgcggagc ggccacgcat ccgggcggat cggtgatggg
     2341 aatcaacggt cgcaacgcgg ccgcgtcggt gctgcgcgac caaggacgat cgctggaggg
     2401 ggcacggtga gcggcgccag cgggccaccc gacccgggtc
//
LOCUS       DATCCH010000354         4951 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_497302, whole genome shotgun sequence.
ACCESSION   DATCCH010000354 DATCCH010000000
VERSION     DATCCH010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4951
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497302"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..975
                     /locus_tag="VIJ44_06730"
     CDS             <1..975
                     /locus_tag="VIJ44_06730"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEY5165627.1"
                     /translation="AVIKAGHGDMPRSARLLARTDLQGKVATTVSPLINAADQSRLAR
                     TIMEKTTGIARDRLLPTYAKVRFSKWFRRRPPKPPADPARDTVAFFPTCLVEYQEPAI
                     GKAVVGVYERNGITCELPDGQVCCGMPWLDAGDTEKFEQHARRNVEALAASVRAGHDV
                     VVPQPTCAYVLKNEVPDFLGTEDAHLVADHTYDVSEYLMARHRDEPLDTEFTGRTYET
                     ITWQAACHYRAQQIGPKSRDLMALTGARVTMVERCSAIDGTWGLRAENLEMAHRVAKP
                     LMDTVANTETDLVAGDCHLSNTAIREATTKVPRHPLQVLAQAYGIVED"
     gene            981..1559
                     /locus_tag="VIJ44_06735"
     CDS             981..1559
                     /locus_tag="VIJ44_06735"
                     /inference="COORDINATES: protein motif:HMM:NF023432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HEY5165628.1"
                     /translation="MRKLTAADIRDLREYERERDAFRSEIITVKKKRRIPLGDLMTIV
                     FENAATMRFQIQEMARAERMLRDEQIANEVETYNELIPADGELSGTLFIEITDKEALQ
                     YWLPRLVGIHEHLQFVIDDTTVLGQAQNVDRLTREKTTTTVHYLKFAFTPAQQRAFET
                     GPVRLVVDHPEYQVDITLSDEQRDELVGDFAA"
     gene            1556..1999
                     /gene="dut"
                     /locus_tag="VIJ44_06740"
     CDS             1556..1999
                     /gene="dut"
                     /locus_tag="VIJ44_06740"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEY5165629.1"
                     /translation="MTLQIHLARLDPDLPLPAQAHAHDAGYDLTARTGADLAAGGGRA
                     LVPTGIAVAIPPGYAGLVLPRSGLALRHGVTCLNTPGLIDPQYRGELMVLLVNTDPHL
                     PYTIGRGDRIAQLVITAVESAIDWVEVDQLDGTARDTFGFGSTGR"
     gene            2064..3008
                     /locus_tag="VIJ44_06745"
     CDS             2064..3008
                     /locus_tag="VIJ44_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03621 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEY5165630.1"
                     /translation="MPHDRKFRFGVMAPRAESHVQYRDAARKWEDLGYSTLFVPDHFV
                     DHPLAPIPAMAMVAEATERLRVGSLVLGNDYKHPVVLAREAATLDLLSDGRLELGVGA
                     GWMTADYEHAGLPLDRPGIRVERLAESLTVLKGLFSDQPFSFKGKHYEITDLDGSPKP
                     VQRPGPPILVGGGGERVLSLAAREADIVGINANLRRGEAQHPDAAQSLSAAATDQKLG
                     WVRSAAGLRFDDLEIQQYAGFVFFTDDRQSLAEAMAPAFDVTPEVALETPIALVGTTE
                     QMIDDLIARRERWQMTYVVIDDAVADQFAPIVARLAGT"
     gene            3023..3964
                     /locus_tag="VIJ44_06750"
     CDS             3023..3964
                     /locus_tag="VIJ44_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03621 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEY5165631.1"
                     /translation="MSTHHPFRFGVQVGGSHSATEWRALAHKIEDLGYSTLFMPDHFI
                     ETELAPMVGIAVAAAATDRLRVGALVLGNDYKHPAVVAKETATLDVLTDGRVELGIGA
                     GWMKTDYEALGMPYDSAGTRINRLAEAITLIKGAWGDGPFDLSGEHYTIRSYNGLPKP
                     VQQPRPPILVGGGGPGLLRLAGKEADIVGINPNLRAGAITSDALKTSLAEQTQEKIGW
                     VREGAGPRFDEIELQIRYFVAAITDDANGLAEAMAPGLGVSATEALQSGVALVGTVDE
                     VCDTLTKRRDEWGVSYIVLGDDTFAAFAPVVARLAGT"
     gene            4044..4119
                     /locus_tag="VIJ44_06755"
     tRNA            4044..4119
                     /locus_tag="VIJ44_06755"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4077..4079,aa:Gly,seq:gcc)
BASE COUNT          875 a         1881 c         1464 g          731 t
ORIGIN      
        1 gccgtcatca aggccggcca cggggacatg ccccgatcgg ctcgcctcct cgcccgcacc
       61 gacctccagg gcaaggtcgc gacgacggtc tcgccactca tcaatgccgc cgaccagtcg
      121 cggctggctc gcacgatcat ggagaagacc accgggatcg cccgggaccg cctcctcccg
      181 acctacgcga aggtgcggtt ctccaagtgg ttccggcgcc gaccaccgaa gccgcccgcg
      241 gaccctgccc gggacacggt cgcgttcttc ccgacctgcc tggtcgagta ccaggagccg
      301 gccatcggca aagccgtcgt cggcgtctac gaacgcaacg gcatcacctg cgagctaccc
      361 gacggccaag tgtgctgcgg catgccgtgg ctcgacgccg gcgacaccga gaagttcgag
      421 caacacgccc gccgcaacgt cgaggcgctc gccgccagcg tccgggccgg ccacgatgtc
      481 gtcgtccccc aaccgacctg cgcgtacgtt ctcaagaacg aggtacccga cttcctcgga
      541 accgaggatg cccacctcgt cgcggaccac acctacgacg tctcggagta cctcatggcc
      601 cgacaccgag acgagcctct cgacaccgag ttcacgggcc ggacctacga gacgatcacc
      661 tggcaggccg cgtgtcacta ccgggcccag cagatcggcc cgaagagccg cgacctcatg
      721 gccctcactg gtgcccgggt cacgatggtg gaacgctgca gcgcgatcga tggaacctgg
      781 ggtctgcggg ccgagaacct cgagatggcc caccgcgtcg cgaagcccct catggacacc
      841 gtcgccaaca ccgagaccga cctcgtcgcc ggtgactgcc acctctcgaa caccgccatc
      901 cgcgaggcca cgaccaaggt gccgcggcac ccgctccaag tgctcgccca ggcctatggc
      961 atcgtggagg actgacgccc atgcgcaagc tcaccgccgc tgacattagg gacctccggg
     1021 agtacgagcg cgaacgcgac gcgttccgat ccgagatcat caccgtaaag aagaagcgtc
     1081 gcatccccct cggggacctg atgacgatcg tgttcgagaa cgcggccacg atgcggttcc
     1141 aaatccaaga gatggctcga gccgaacgga tgctgcgcga cgagcagatc gccaacgagg
     1201 tcgagaccta caacgagctc atccccgccg acggtgagct ctccgggacg ctgttcatcg
     1261 agatcaccga caaggaagcc ctccagtact ggcttccgcg cctggtcggc atccacgagc
     1321 acctccagtt cgtgatcgac gacaccacgg ttctaggaca ggcccaaaac gtcgaccgtc
     1381 tcacccgcga gaagaccacg accaccgttc attacctcaa gttcgcgttc acccccgcac
     1441 agcagcgggc cttcgagacc ggtccggtcc gactcgtcgt cgaccatccc gagtaccagg
     1501 tcgacatcac cctcagcgac gagcagcgag acgaacttgt cggagacttc gccgcttgac
     1561 cctccagatc catctcgccc ggctggaccc ggacctgccc ctccccgctc aggcgcacgc
     1621 ccacgacgcg ggctacgacc tgaccgcccg taccggcgcc gacctcgccg ccggcggtgg
     1681 tcgtgccttg gtccccaccg gcatcgccgt cgcgatcccc cccggctacg ccggcctggt
     1741 gctgccccgc tccggcctcg cgctgcgtca cggcgtgacg tgcctcaaca cgcctggcct
     1801 gatcgacccg cagtaccgcg gcgagctcat ggttctgctc gtcaacaccg acccccattt
     1861 gccctacacc atcgggcggg gagaccggat cgcccagctg gtgatcaccg cggtcgaaag
     1921 cgcgatcgac tgggtcgagg tcgaccagct cgacggcacc gcccgcgaca ccttcgggtt
     1981 cgggtcaacc ggtcgctgag cccgacccgt cgtcaactcg acggcagccg cgctgctcga
     2041 cccgcggcca ttagcgtgcc gccgtgcctc acgaccgcaa gttccggttc ggtgtcatgg
     2101 ccccccgcgc cgaatcccac gtccaatacc gcgacgcagc ccgcaaatgg gaagacctcg
     2161 ggtactcaac cctcttcgtc cccgaccact tcgtcgacca tcccctcgcg ccgatcccag
     2221 ccatggcgat ggtcgccgaa gcaaccgaac ggctccgcgt cggatcactc gtactcggca
     2281 acgactacaa gcatcccgtc gtgctcgccc gggaggcagc caccctcgac ctgctctcgg
     2341 acggtcgcct cgagctcggc gtcggcgccg gctggatgac ggcggactac gaacacgcgg
     2401 gattgccgct cgatcgaccc ggcatccgcg tcgagcgtct cgccgaatcc ctcacggtcc
     2461 tgaagggcct cttctccgac cagccgttca gcttcaaggg caagcactac gagatcacgg
     2521 acctggacgg ctctccaaaa ccggtgcagc gccccggacc accgatcctc gtcggaggcg
     2581 gcggggaacg cgtcctctcc ctcgcggcac gcgaagcgga catcgtcggc atcaacgcca
     2641 accttcgtcg aggcgaagcc cagcatcccg acgccgccca gtccctcagc gccgccgcga
     2701 ccgaccagaa gctcggctgg gtgcgatccg ccgccggtct ccgattcgat gacctcgaga
     2761 tccagcaata cgcgggcttc gtcttcttca ccgatgaccg ccagtcactc gccgaagcaa
     2821 tggccccggc cttcgacgtc acccccgagg tcgccctcga gacaccgatc gcgctggtcg
     2881 gcaccaccga gcaaatgatc gacgacctca tcgcccgccg cgaacgctgg caaatgacct
     2941 acgtcgtcat cgacgacgcc gtcgccgacc agttcgcgcc catcgtcgcc cggctcgccg
     3001 gcacctgaga gaaggaccag ccatgagcac ccaccacccg tttcgcttcg gtgtccaggt
     3061 cggcggatcc cactccgcca ccgagtggcg agccctcgcc cacaagatcg aggacctggg
     3121 ttactccaca ctcttcatgc ccgaccactt catcgagacc gagctggcac ccatggtcgg
     3181 gatcgcggtc gcggccgccg ccaccgaccg tctccgagtc ggcgcgctcg tcctcggcaa
     3241 cgactacaag catcccgccg tcgtcgccaa agagaccgcc acgctcgacg tcctgaccga
     3301 cggacgcgtg gagctgggaa tcggtgccgg atggatgaag accgactacg aggcgctcgg
     3361 aatgccgtac gactccgccg gcacccgcat caaccgactc gccgaggcga tcacactcat
     3421 caaaggcgcc tggggagacg ggcccttcga tctcagcggc gagcattaca ccatccgctc
     3481 ctacaacggc ctccccaagc ctgtccagca gcctcgaccc ccgatcctcg tcggcggagg
     3541 cggtccaggg ctgcttcgcc tcgctggcaa ggaggccgac atcgtcggaa tcaaccccaa
     3601 cctccgcgct ggtgccatca ccagcgacgc cctgaagacc tcgctcgccg agcagaccca
     3661 agagaagatc ggctgggtgc gcgaaggcgc cggtccccgc ttcgacgaga tcgagctcca
     3721 gatccggtac ttcgtcgcgg ccatcaccga cgacgccaac ggcctcgccg aggcaatggc
     3781 accgggcttg ggcgtgagcg ccaccgaagc gctccagtcc ggcgtcgcgc tcgtcggcac
     3841 cgtcgacgag gtctgcgaca ccctgacgaa acgtcgagat gaatggggcg tctcgtacat
     3901 cgtgctcggc gacgacacct tcgccgcctt cgctcccgtt gtcgcgcgcc tcgccggaac
     3961 ctagcgcggc gccacccaga cctcggcacg gcgtcgcgcc tcgcccgccc tccgcaacct
     4021 ccgcgctatt ctcgttggct cacgcggacg tggctcagct ggtagagcat caccttgcca
     4081 aggtgagggt cgcgggttcg aatcccgtcg tccgctccac agaacctgtg gggtgtcaaa
     4141 actcggccgc gcccctgacc agcggttcga catcgtcgcg tcctaggccg ggtcgcgtct
     4201 tcgagctccg cggcgaatcc catccgctgc accagcctca gtacgcactc ccccagtcgc
     4261 ggcaatgccc attgtggggt cgccaacctc ccggtgaatt cccaggtcag cagcgtgatc
     4321 gagttttcgc accccacagg cgaagcgcag ggaatcacca accgtgggca cgtccgggtt
     4381 gaccggtgat cgccgcctgc ggctcgtcgg gacaggcgct gcggatcaca aggtgcgctc
     4441 ctttgctggg gcggagctgc gcctggggtt caaactggtt ggcccatacg ccggtggcgt
     4501 tgacgccgtg gcgggcccgg agcgtgaact cgtcgccgct gcgttcgtcg cgaaccagct
     4561 gggtcgcgct cgactgacaa ggtgccgcgg actacgccaa gcgctgaccg gcgagccaaa
     4621 cccgacacca caatccgctc acgccgcggg gtgcccgtcc cactaaccgc ccgtctcgtc
     4681 ctccgcgcat tcaatggtgt cggcgagtag atcacgccct gcggaaccga tccggcttcg
     4741 cctggttgcg cgcgtctcag tagcgccgcg acgagcaact caccggcggc gcgtcgatcc
     4801 tcgctgtgga caagccaaac gtcggcttcg agctcggcag cgcgttggcg tagctcggcg
     4861 gcatccatac ccggcacaac ttggaggcca cgcgacaccg aaccggcaga cggaagggcc
     4921 ggaacggcga tcgcgccgtg ggctacatcg t
//
LOCUS       DATCCH010000355         3460 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_435056, whole genome shotgun sequence.
ACCESSION   DATCCH010000355 DATCCH010000000
VERSION     DATCCH010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435056"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..2115)
                     /gene="tkt"
                     /locus_tag="VIJ44_06760"
     CDS             complement(118..2115)
                     /gene="tkt"
                     /locus_tag="VIJ44_06760"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEY5165632.1"
                     /translation="MPVTDLEQQGINVVRALAMDAVQRANSGHPGTPMALAPLAHVLF
                     TRVMRYDASQPAWPNRDRFVLSNGHASMLLYSFLYLTGFGLELDDLRQFRQWESRTPG
                     HPEYRHAPGIEVTTGPLGQGFANSVGIALAEANLRGRFGPEVSDHHTFVIAGDGDFEE
                     GISHEAASLAGHLGIGRLTCVYDDNHITIDGPTELAYSDNVPERFEAYGWHVVQLGEA
                     ANDVDTLERGLREAMAEADRPSLVVLRSHIGWPSPKYTDTAHAHGSPLGEDEIRTVKA
                     ILGLPPDETFWVPDEVLTSYRDAGLRGKPDREAWEQRRQALRDREPALADKFDACLDQ
                     RGLPGWEAKIPSWAPGEQLATRVASNQVLDAIVDVVPGIVAGGADLTGNTGTELKDEG
                     VVAEHQFRGRQLHFGIREFGMGGVMNGLAVSYLMPAGGTFFVFSDYMRGAARLAALSG
                     YKTLFVWTHDSVGLGEDGPTHQPIEQLAAFRAMPGLRLIRPADPNEVAQAWRLHVSGD
                     GPTALILTRQKVPVLEGSAERAPDGVPRGAYTLVDEPGGLDLVLVGTGSEVSVCVAAQ
                     DLLAAEGRSVRIVSMPSWDLFAAQPEQYRHQVLPPGVSKLAVEAASSFGWDRWVDDVV
                     SIDRYGASAPGPVVMDHLGINPAHVAERARALLARPPKGTP"
     gene            complement(2274..3413)
                     /locus_tag="VIJ44_06765"
     CDS             complement(2274..3413)
                     /locus_tag="VIJ44_06765"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HEY5165633.1"
                     /translation="MNRYASALSCHPVAAHAVGEAAGEILEAFDGDDPSLVVCFVSPH
                     FVGAFDDAANALRNLLDPQVLLGVTAGGIIGGSREVEDGPAFSVFAAHLPDATLTPVA
                     LQTEDTPDGSAITGWPETPGTAPTLLLAADPFTFPADAFLRRLNEDRPGLSVIGGLAS
                     AASHPGGNRLILDDQVRADGAVGVFLDGVPVDTVVSQGCRPIGQPFTVTRADRNLVGE
                     LAGQPALQRLQELAAAVSDDERELLRRGLHVGVVVDEHKVDFGRGDFLVRNVLGADPD
                     AGTIAVGDVVDVGQTVQFHVRDGQAADEDLRALLHTCRADGALLFTCNGRGQHLFGVP
                     DHDSGVVAELLGSVPLAGAFCAGELGPVGGRNFLHGFTASLALFH"
BASE COUNT          509 a         1175 c         1265 g          511 t
ORIGIN      
        1 cccggatgcc gtcttcgtcg atgagctgcc gtaagccgcc cccggtgacg agcggtcgcg
       61 tgaggttgtc gtaccagggg ctctggccga agtcattgag tcgggcgatg gcgctggtca
      121 tggtgttccc ttcggggggc gagcgaggag cgcgcgggca cgctcggcga cgtgcgcggg
      181 gttgatcccg aggtggtcca tcacgaccgg gcccggtgcc gacgccccgt accggtcgat
      241 gctgaccacg tcgtcgaccc agcggtccca accgaacgac gaggcggcct cgaccgcgag
      301 cttcgacacc ccggggggca gcacctggtg gcggtactgc tcgggctggg cggcgaagag
      361 atcccacgac ggcatcgaga cgatccgcac cgaccggcct tcggccgcga gcaggtcttg
      421 agccgcgacg cacaccgata cctccgagcc cgtgccgacc aggacgagat cgagaccgcc
      481 gggctcgtcg acgagggtgt aggccccgcg ggggacaccg tcgggggcgc gctcggcgct
      541 gccttcgagg acggggacct tctggcgggt gaggatgagc gcggtggggc catcgccgct
      601 gacatgcagc cgccacgcct gggcgacctc attggggtcg gcggggcgga tcagacgcag
      661 gccgggcatc gcgcgaaacg ccgccagctg ctcgatcggc tggtgggtcg gcccgtcctc
      721 accgagtccc accgagtcgt gggtccagac gaacaaggtc ttgtagccgg acagcgcggc
      781 gaggcgagcc gcgccgcgca tgtagtccga gaacacgaag aacgtgcctc ccgcgggcat
      841 gaggtacgag accgcgaggc cgttcattac gccgcccatc ccgaactcgc ggatcccgaa
      901 gtgcagttgg cgcccgcgga actgatgctc ggcgacgacg ccttcgtctt tgagctcggt
      961 gccggtgttc cccgtcagat cggcgccgcc ggcgacgatt ccgggtacga cgtcgacgat
     1021 cgcgtcgagg acctggttgc tcgcgacccg cgtcgcgagc tgctcgccgg gcgcccatga
     1081 tgggatcttc gcttcccagc cgggcaggcc gcgctggtcg aggcaagcgt cgaacttgtc
     1141 ggcgagggcc ggttcccggt cgcgcagcgc ctggcgtcgc tgctcccagg cctcgcggtc
     1201 gggcttccct cgcagcccgg cgtcgcgata gctggtcagc acctcgtcgg gcacccagaa
     1261 cgtctcgtcg ggtggcagac ccaggatcgc cttgacggtc cggatctcgt cctctccgag
     1321 cgggctgccg tgcgcgtggg cggtgtcggt gtacttcggt gatggccatc cgatgtggct
     1381 gcgcaggacg acgaggctgg gccggtcggc ctcggccatc gcctctcgca gacctcgctc
     1441 gagcgtgtcg acgtcgttgg cggcctcacc gagctggacg acatgccagc cgtaggcctc
     1501 gaagcgctcg gggacgttgt cgctgtacgc cagctctgtc ggcccgtcga tggtgatgtg
     1561 gttgtcgtcg tacacgcagg tgagccggcc gattccgagg tgaccggcga gggacgcggc
     1621 ttcatggctg attccttcct cgaagtcgcc gtcgccggcg atgacgaagg tgtggtggtc
     1681 gctgacctcc ggtccgaacc gcccgcgcag gttcgcctcc gccagcgcga tgccgacgct
     1741 gttcgcgaac ccttggccga gcggcccggt tgtgacttcg atgccgggag cgtgccggta
     1801 ttcggggtgt cccggggtgc gtgattccca ttggcgaaac tggcgcaagt cgtcgagctc
     1861 gagcccgaag ccggtcagat agaggaagga gtagaggagc atcgacgcgt gcccgttcga
     1921 gagcacgaac cggtcgcggt tcggccacgc gggctggctg gcgtcgtagc gcatcacgcg
     1981 ggtgaacagc acgtgcgcaa ggggagcgag cgccatcggg gtgccgggat ggccggagtt
     2041 ggctcgttgg acggcgtcca tcgccaaggc gcgcacgacg ttgattccct gctgctccag
     2101 gtcggtcacc ggcactggcg ccctcctcgc tcgtgccctc gccaggcccg gctccgtgac
     2161 ccggccgcgg gcgtggcggg gtcgccaccg atccgttccg cgacgccgcc gcggtagccc
     2221 ccactatggc gcgtcatcga cgtccccccg gcgtgcgacg cggtggggga ccgtcagtga
     2281 aagagcgcga ggctggcggt gaagccgtgt aggaagttgc ggcccccgac gggtccgagc
     2341 tccccggcgc agaacgcgcc cgccaacggg accgacccga gcagttcggc gacgacccca
     2401 gaatcgtggt cggggacgcc gaagaggtgc tggccccgcc cgttgcaggt gaacaggagc
     2461 gcaccgtcgg cccggcaggt gtgcagcagc gcccgcaggt cctcgtcggc ggcctggccg
     2521 tctcgcacgt gaaactgcac cgtctggccg acgtcgacga catcgccgac tgcgatggtc
     2581 cccgcgtcgg gatcggcgcc gaggacgttg cggacgagga agtcaccccg gccgaagtcg
     2641 accttgtgct cgtcgacgac gacacccacg tgaaggcccc gccgaagcag ctcacgctcg
     2701 tcgtcgctca ccgccgcggc gagttcctgg aggcgctgca gggccggctg gccggcgagc
     2761 tcccccacaa ggttgcggtc agcgcgcgtc acggtgaacg gctgaccgat tgggcgacac
     2821 ccctgtgaca cgacggtgtc gaccggaacg ccgtcgagga acacaccgac cgcgccgtcg
     2881 gctcggacct ggtcgtcgag aatgagacgg ttgccgccgg gatgactggc ggccgaggcc
     2941 agcccgccga tcacggaaag accggggcgg tcctcgttga ggcgacgcag gaacgcgtcc
     3001 gccgggaacg tgaacggatc ggccgcgaga agcaacgtgg gtgcggttcc cggtgtctcc
     3061 ggccatccgg tgatcgccga cccgtccggc gtgtcctcgg tctgcagcgc gacgggcgtc
     3121 agcgtggcgt cgggaaggtg cgcggcgaag accgagaacg cggggccgtc ctcgacctcg
     3181 cgggagccgc cgatgatccc accggcggtg actccgagga gcacctgggg atcgagcagg
     3241 ttgcgcagcg cgttcgcggc gtcgtcgaac gcccccacga agtgcggcga cacgaagcac
     3301 accacgaggc tcggatcgtc accgtcgaac gcctcgagga tctcgcccgc ggcttcaccg
     3361 accgcgtgcg ctgccaccgg atgacacgac aacgccgagg cgtaccggtt caccacgtca
     3421 acctactggg ccgaccccgg tccgtcggcg gggagtcgct
//
LOCUS       DATCCH010000356         6799 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_123252, whole genome shotgun sequence.
ACCESSION   DATCCH010000356 DATCCH010000000
VERSION     DATCCH010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6799
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123252"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="VIJ44_06770"
     CDS             <1..761
                     /locus_tag="VIJ44_06770"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5165634.1"
                     /translation="TFLFDEMPSFRDTLTLPAAERSSQLRDPVRREQMRRELANPSGR
                     SFVFVWSVLRVETVRHDRNRRWLDRSVAEIADELGTDPLDAFLDLSLDEDLETQFVLA
                     APPDPKRRAATETMIRAPFVMAGSSDGGAHLLSFCGADYTTRLLTEWVPDVLTIEAAV
                     ARLTSIPARAVGLTDRGVLTPGAPADVLVIDRERLSTPDAPRYVEDFPAGSGRFVADA
                     DGYAKVIVNGEILFEDGTWTGATPGHIVRGGASP"
     gene            845..2125
                     /locus_tag="VIJ44_06775"
     CDS             845..2125
                     /locus_tag="VIJ44_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013426651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5165635.1"
                     /translation="MQRDDMIIVSVDDHVVEPPDLFERHLPDRYRDLAPRVVHQDDGT
                     DVWEFHGLRVPNIGLNAVAGRPPEEYGIDPTSFDELREGCFDVEKRVLDMSANGVLGS
                     LNFPSLPGFAGRLFGALEDKDAARILTEAYNDWHIDDWCGHAPERFIPLAVPAIWDPE
                     VTAAEIRRVAKKGCHAITFPENPVPLGLPSLHSDHWDPVWQAASDTGMIVCMHIGSSS
                     KLVVTAPDAPMDVMMVLQPMNIVQCAADLVYSPIWKKFPDVTVALSEGGIGWIPYFLE
                     RLDHTYRTHRAWTGVDFGDQTASEFFLDHVMLCFITDPVGLKLVRDHIGVERVTVEID
                     YPHSDTTWPNAPERLMGEFAGTDLTDAEIDAITHENAMRYFQYEPFAIRPREQCTVGS
                     LRAEAIGVDTKPVARGRRFPERHGITVNKLTPTA"
     gene            2200..2373
                     /locus_tag="VIJ44_06780"
     CDS             2200..2373
                     /locus_tag="VIJ44_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165636.1"
                     /translation="MHWLVDAAVVFLATIIVALFLGVPWWAVLIIAFVLGSFGARLTY
                     RADVRAMAERRSG"
     gene            complement(2401..3600)
                     /locus_tag="VIJ44_06785"
     CDS             complement(2401..3600)
                     /locus_tag="VIJ44_06785"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5165637.1"
                     /translation="MTVVAPDRFVDADGHVLEHPDGMLKHAPAEYRNRIWHVETDADD
                     RDWVVFDGARDPANVFAYAGSGGLPPEEQARAAAGAIRYADIRPGAFEPMPRLADLDA
                     ERIDQTVLYPTNLLGIAGIPDLDFAIAQCRAYNDWLSDFCSAAPDRLFGIAIIPQQDP
                     DAAARELRRAATKPNLVGTFIRPNPVVEWKHFLDPVWDPIWQAASDTGWPLGLHPFLA
                     GDVPGAVLGLRLNTIKARTGMTMNAGLNMDNIFFTQCIGNPVDMMTSLAFILAGGVCE
                     RFPDLTLIVLETNGGWITPWMERLDHHYKTFGFDVPDIRMAPSEYFRRQCYVSFDADE
                     ETLTLAAESPYVGADRIIWASDYPHPDAKFPGTVAELLNNIGGMDAESQRAIAAGNAQ
                     RLYRLPG"
     gene            3645..4358
                     /locus_tag="VIJ44_06790"
     CDS             3645..4358
                     /locus_tag="VIJ44_06790"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY5165638.1"
                     /translation="MPATVVLVHGACHGAWCWDKVVDGLTVRGIPSVAVDLPGHGESR
                     DALGDLATDAAALRATLDSFDRSVVCGHSYGGAVITEGAAGHPGVQHLVYITAFALEV
                     GESTMAAASDGSEADTATKLGEAMLFADDGTVTFQAPAVVDALYHDCTPADIEFALAR
                     LGPQTLSSLGGVATRAAWHDIPSTYAICTEDRGVAPELQHRLAARTNETVEWDTSHSP
                     FLSRPDLVVDLLAGIAERT"
     gene            4369..4761
                     /locus_tag="VIJ44_06795"
     CDS             4369..4761
                     /locus_tag="VIJ44_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165639.1"
                     /translation="MSTLEDYVAKAQAALDTLRPQLDELRVQADLAQAEARDRLEAGI
                     AALQKAQAAAKAQLDQAARAGKDTWKSTAQQAEQAVDDLGTQLQQVAEQVQKSVGAAA
                     PAAQKGWRTFLDEWNRERQDRQQLLDED"
     gene            4758..5516
                     /locus_tag="VIJ44_06800"
     CDS             4758..5516
                     /locus_tag="VIJ44_06800"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY5165640.1"
                     /translation="MTSAHERRNREFWDADADDYQAAHAADLRPERARAWGVWRIPEA
                     ELQVLGPVRDRDVLEYGCGAAQWSIALAREGARPTGLDQSRSQLHHASRLQHEQRITF
                     PLVAASAEATPFAAASFDLVFCDHGAMSFCDPAATIPEVARLLRPDGRLVFSKTTSLV
                     YLTFDWARDRQDIRLHHDYFGMRVFDTGEGTVDFQVPYGDMVRTVRQHGFLMEDLVEL
                     RAPAGATTTYDEFVPADWARRWPAEEIWVLRREG"
     gene            complement(5713..>6799)
                     /locus_tag="VIJ44_06805"
     CDS             complement(5713..>6799)
                     /locus_tag="VIJ44_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013426362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY5165641.1"
                     /translation="CLIYTAGTTGPSKGCMLPHNYIVSLADQIARAWQRRPDDVVLTP
                     LPLFHFNAISICVVGTLVTGGTAGIARRFSVSQFWPEVQRTKATIVSLLGSLAILIAD
                     AADHPDQPGHRLRLCAAAPIPPDTDRIWRRRFGCATFSGGYGLTEASLISLLPAGQTN
                     QPGAAGKPNRHDFDVAIVDDDDHEVPVGATGEIVCRPRRPNVMFAGYWGRADDTLAVL
                     RNLWFHTGDLGRLDTDDFLYFVDRKKDYLRRRGENISSFELERIFHDHEAIQDVAVHS
                     VPSDVGEDDVKVTVVLIDEASITEEALCRWAIDHVPYFAVPRYIEFRPDLPRNPVGRV
                     LKYQLREDGVTPTTWDREAAGVTFERR"
BASE COUNT         1075 a         2294 c         2328 g         1102 t
ORIGIN      
        1 ccactttctt gttcgacgag atgccgagct ttcgtgacac tctcaccctc cccgccgcgg
       61 aacgctcatc ccagctacgc gaccctgtcc gacgcgagca gatgcgccgt gagctcgcca
      121 acccgtccgg ccgctccttc gtcttcgtgt ggtcggtcct tcgcgtcgag acggtccgcc
      181 acgaccgcaa ccgccgctgg ctcgaccgca gcgtcgctga gatcgccgac gagctcggca
      241 ccgaccccct cgacgcgttc ctcgacctgt ctctcgacga ggacctcgag acccagttcg
      301 tgctcgccgc gccaccggac ccgaagcggc gggccgccac cgagaccatg atccgcgccc
      361 cgttcgtgat ggccggcagc agcgacggcg gcgcccacct cctctccttc tgcggcgccg
      421 actacaccac ccgcctgttg accgaatggg tgcccgacgt gctcaccatc gaggccgcgg
      481 tggcgcggct cacctcgatt ccggcccggg cggtcggcct cacggaccga ggcgtgctca
      541 ccccgggtgc ccccgccgac gtgctcgtca tcgaccggga acggctctca acccccgacg
      601 cgccccgtta cgtcgaggac ttcccggccg gaagcggtcg cttcgtcgcc gacgccgacg
      661 gttacgccaa ggtcatcgtc aacggcgaga tccttttcga agacgggacc tggaccgggg
      721 cgacgcccgg acacatcgtg cgcgggggcg ccagcccgta atcgttggtg ccgacgtcat
      781 cggcagggcg ccagacgatg cggcgctacc ctgacgggcg cgtcacgaac cgtggaggac
      841 cgccatgcag cgcgacgaca tgatcatcgt cagcgtcgac gaccacgtcg tcgagccacc
      901 cgatctcttc gagcggcacc tccccgaccg gtaccgggac ctcgcccccc gggtggtcca
      961 tcaggacgac ggcaccgacg tctgggagtt ccacgggttg cgggtcccca acatcggcct
     1021 caacgccgtc gccgggcggc cccctgagga gtacggcatc gacccgacct cgttcgacga
     1081 gcttcgcgaa gggtgcttcg acgtcgagaa gcgggtgctc gacatgagcg ccaacggcgt
     1141 cctcgggtcg ctcaacttcc cgtccctgcc cggcttcgcg gggcggctct tcggtgcgct
     1201 cgaggacaag gacgcggccc ggatcctcac cgaggcctac aacgactggc acatcgacga
     1261 ctggtgcggg cacgcgccgg agcgcttcat cccgctcgcg gtgcccgcga tctgggatcc
     1321 cgaggtgacc gcggcggaga tccgacgggt ggcgaagaag ggctgtcacg ccatcacgtt
     1381 cccggagaac ccggtgccgc tcggcctgcc gagcctgcac tcggatcatt gggatccggt
     1441 gtggcaggcc gcgtccgaca ccgggatgat cgtctgcatg cacatcgggt cgtcgtcgaa
     1501 gctggtggta accgcgccgg acgcgcccat ggacgtgatg atggtcctgc aaccgatgaa
     1561 catcgtgcaa tgcgcggccg atctcgtgta ctcgccgatc tggaagaagt tcccggatgt
     1621 gaccgtggcg ctgtccgagg gcgggatcgg atggatcccg tatttcctgg agcggctcga
     1681 tcacacgtat cggacccacc gggcctggac cggggtggac ttcggggacc aaaccgcgag
     1741 cgagttcttc ctcgaccacg tcatgctctg cttcatcacc gacccggtgg ggttgaagct
     1801 cgtccgggac cacatcggcg tggagcgggt caccgtcgag atcgactatc cgcactccga
     1861 cacgacctgg ccgaacgcgc ccgagcggct catgggcgag ttcgccggca ccgacctgac
     1921 cgacgccgag atcgacgcca tcacccacga gaacgcgatg cggtacttcc agtacgaacc
     1981 gttcgcgatc cgaccccgag aacagtgcac cgtgggttcg ctgcgggcgg aggcgatcgg
     2041 cgtggacacc aagcccgtcg cccgcggccg gcgtttcccc gagcggcacg gaatcaccgt
     2101 caacaagctg acgcccacgg cgtgagtgca acgccaagcg cgggaggctc tgacggtgag
     2161 ggaccggctc cgctccggac ccagcctcgt gcgttcatcg tgcattggct cgtggacgcc
     2221 gcggtcgtgt tcctcgcgac gatcatcgtg gcgttgttcc tcggggtgcc ctggtgggcg
     2281 gtgctcatca tcgcgttcgt gctcggcagc ttcggtgccc gactcaccta tcgcgccgac
     2341 gtgcgcgcga tggccgagcg tcgcagcggc tgagcgacgg gcattcggcg cccgggtggc
     2401 tcaacccggg aggcggtaga gcctctgggc gttgcccgcg gcgatcgctc gctgggactc
     2461 cgcatccatg ccgccgatgt tgttcagcag ctcggcgacc gtcccgggga acttggcgtc
     2521 gggatgcggg tagtccgacg cccagatgat ccggtctgcg ccgacatacg gcgactctgc
     2581 ggcgagggtc agggtctcct cgtcagcgtc gaaggacacg tagcactgcc gacggaagta
     2641 ctccgacggt gccatccgga tgtcgggcac gtcgaatccg aacgtcttgt agtggtggtc
     2701 gagccgttcc atccacggcg tgatccagcc gccgttcgtc tccagcacga tgagggtgag
     2761 atcggggaag cgctcgcaga caccaccggc caggatgaac gccaggctcg tcatcatgtc
     2821 gaccgggttg ccgatgcatt gagtgaagaa gatgttgtcc atgttgagcc cggcgttcat
     2881 cgtcataccg gtcctcgcct tgatggtgtt gagccgaagg ccgaggaccg cgccggggac
     2941 atcgccggcc aggaacgggt ggagaccgag gggccagccg gtgtccgacg cggcctgcca
     3001 gattggatcc cagaccgggt cgaggaagtg cttccattcc acgaccgggt tcggccggat
     3061 gaaggtcccg accaggttgg gcttggtcgc ggcgcgccgg agctctcggg ccgcggcgtc
     3121 gggatcttgc tgcgggatga tcgcgatccc gaacaggcgg tccggcgccg cggagcagaa
     3181 gtcgctcaac cagtcgttgt aggcgcggca ttgcgcaatc gcgaagtcga gatcgggtat
     3241 gcccgcgatg ccgaggaggt tcgtcgggta caagacggtc tggtcgatgc gttccgcgtc
     3301 gaggtctgcc agtcgcggca tgggctcgaa cgctccggga cgaatgtcgg cgtatcggat
     3361 cgctccggca gcggcgcggg cctgctcctc tgggggcagg ccgcctgacc cggcgtaggc
     3421 gaacacgttc gcggggtcgc gggcaccgtc gaacaccacc cagtcccggt cgtcggcgtc
     3481 ggtctcgaca tgccagatcc ggttccgata ctcggccggc gcgtgtttga gcatcccatc
     3541 ggggtgctcg aggacgtgac cgtcggcgtc gacgaaccgg tccggagcta ccaccgtcat
     3601 gtttcctcct gcgtggtcga tgcttgcttg tctagtctgg ctcgatgccg gcaaccgtcg
     3661 tgttggtgca cggcgcctgt cacggcgcct ggtgctggga caaggtcgtg gacggcctca
     3721 cggtccgtgg gatcccgtcg gtggccgtcg atcttcccgg tcacggtgag agccgcgacg
     3781 ccctcgggga tctcgcgacc gacgcggccg ccctgcgcgc caccctggac agcttcgacc
     3841 ggtcggtcgt gtgcggccac tcctacggcg gcgcggtgat caccgaaggc gccgccggac
     3901 atcctggcgt gcagcatctc gtctacatca ccgcgttcgc gttggaggtc ggggagtcga
     3961 ccatggccgc ggcatccgac ggctccgaag cggacaccgc gacaaagctg ggggaagcca
     4021 tgttgttcgc cgacgacggg acggtgacct tccaggcacc ggccgtcgtc gacgcgctgt
     4081 accacgactg cacgccggct gatatcgagt tcgcgttggc tcgcctcggg ccccaaaccc
     4141 tcagctctct gggcggcgtc gcgactcgcg ccgcgtggca cgacatcccg agcacctacg
     4201 cgatctgcac cgaggaccgg ggggtggcac ctgagctgca gcaccgcctc gcggcccgca
     4261 ccaacgagac cgtggagtgg gacaccagcc actcaccatt cctgtcccga ccggacctcg
     4321 tcgtggacct gctcgcgggg atcgcggaac gcacctaaga tcggcgcgat gagcaccctc
     4381 gaggactacg tcgcgaaagc ccaagccgcg ctcgacactc tgcgacccca gctcgacgaa
     4441 ctccgggtcc aagcggacct ggcgcaggcc gaggctcgcg accggctcga agccggcatc
     4501 gccgcgttgc agaaggccca agccgcggcc aaagctcagc tcgaccaggc ggcgcgcgcc
     4561 gggaaggaca cctggaagtc gaccgcccag caggccgagc aagcggtcga cgacctcggg
     4621 acccagctgc aacaagtcgc ggagcaggtg cagaagtcgg tcggggcggc ggcgcccgcg
     4681 gcccagaagg gctggcggac gttcctcgac gagtggaacc gtgagcgtca ggatcgtcag
     4741 cagctgctcg acgaggattg acgtccgctc acgagcgacg caaccgggag ttctgggacg
     4801 cggacgccga cgactatcaa gccgcgcacg cagctgatct ccgaccggaa cgggctcgcg
     4861 cctggggagt gtggcggatc cccgaagccg agctgcaggt cctcggtcct gtccgcgacc
     4921 gagacgtcct cgaatacggg tgcggcgccg cgcagtggtc gatcgcgctg gcgcgcgagg
     4981 gggcgcgtcc gaccgggctt gaccagtcgc ggtcccagct ccaccacgcg agtcgcctcc
     5041 aacacgagca acggatcacg ttccccctcg tcgccgccag cgccgaagcc acgccgttcg
     5101 cggccgcgtc gttcgacctc gtgttctgcg accacggggc aatgtcgttc tgtgacccgg
     5161 cggccacgat cccagaggtg gcgcgcctct tgcgcccgga cggccggctc gtcttcagca
     5221 agacgacgtc gctggtgtac ttgacgttcg actgggcgcg cgaccgccag gacatccggc
     5281 tccaccacga ctatttcggg atgcgggtct tcgacaccgg cgagggcacc gtcgacttcc
     5341 aggtcccgta cggcgacatg gtacggaccg ttcgtcagca tggcttcctg atggaagacc
     5401 tcgtcgagct gcgcgctcct gctggcgcga cgacgaccta cgacgagttc gtccccgcgg
     5461 actgggctcg acgctggccc gccgaggaga tctgggtcct tcggcgagaa ggctgacgct
     5521 caccgggcgc gacggtgggg tggttctaac ccaacgccag ttccgctcga gctcctcagc
     5581 gtccctgggc gtcgagaggc cgcctcgggt accagcacac caatcgagga ctccacgatg
     5641 ccagtctcgt cgacgcgcgc atcgaccgtg tcaactcctc agagcggcgt tctgggacga
     5701 gccgcgtgcc gatcagcggc gctcgaacgt cactccagca gcctcgcgat cccaggttgt
     5761 cggtgtaacg ccgtcttctc gtagctggta cttgaggacc cgcccgaccg ggttgcgtgg
     5821 aaggtcgggt cggaactcga tgtagcgggg tacggcgaag tagggcacat ggtcgatcgc
     5881 ccaccggcag agtgcttctt cggtgatgga tgcctcgtcg atcaggacca cggtgacctt
     5941 gacgtcatcc tcaccgacgt cgctcggcac cgagtggacg gcgacgtcct ggatcgcctc
     6001 gtgatcgtgg aagattcgtt ctagttcgaa actggagatg ttctcgccgc ggcgtcggag
     6061 gtagtccttc ttgcggtcga cgaagtagag gaagtcgtcg gtgtcgaggc ggccgaggtc
     6121 gccggtgtgg aaccagaggt tgcggagcac agcgagggtg tcgtcggcgc ggccccagta
     6181 tccggcgaac atgacgttgg ggcgccgagg gcggcagacg atctcgccgg tagcgccgac
     6241 cgggacctcg tggtcgtcgt cgtcgacgat cgcgacatcg aagtcgtgac ggttgggctt
     6301 cccggctgct ccgggctggt tcgtctgtcc tgcggggagc agcgagatca gggacgcttc
     6361 ggtcaggcca tatccgccgc tgaaggtcgc gcacccgaat cgccggcgcc agatgcggtc
     6421 ggtgtcggga gggattgggg ccgccgcaca caaccgcagt cggtgtccgg gttggtcggg
     6481 gtgatcggcg gcgtccgcga tgaggatggc gagcgagccg agcagcgaca cgatcgtcgc
     6541 cttcgtgcgc tggacctcgg gccagaactg cgagaccgag aaccgtcggg cgatgccggc
     6601 agtccctccg gtcacgaggg tgccgaccac gcaaatcgag atggcattga aatggaacag
     6661 cggaagcggg gtgagcacca catcgtcggg tcggcgctgc catgcccgag cgatctggtc
     6721 ggcgaggctc acgatgtagt tgtgcgggag catgcaccct ttcgacggac cggtcgtccc
     6781 tgcggtgtag atgaggcag
//
LOCUS       DATCCH010000357         3009 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_123333, whole genome shotgun sequence.
ACCESSION   DATCCH010000357 DATCCH010000000
VERSION     DATCCH010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123333"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="VIJ44_06810"
     CDS             <1..421
                     /locus_tag="VIJ44_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018149576.1"
                     /note="activates fatty acids by binding to coenzyme A; in
                     Mycobacterium may be involved in virulence; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEY5165642.1"
                     /translation="GTFRVFDGVRYSVPGDWATVEDDETITLLGRGSVCINSGGEKIY
                     PEEVEEAIKRDPTVADCVAVGVPDERWGEAVTAVVAAEPGATVDPERLKAAGAALARF
                     KRPRHIVVVEQIRRGPNGKVDYGWARSTAAERVAKKS"
     gene            complement(474..767)
                     /gene="groES"
                     /locus_tag="VIJ44_06815"
     CDS             complement(474..767)
                     /gene="groES"
                     /locus_tag="VIJ44_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEY5165643.1"
                     /translation="MKLQPLEDRIVVRSNEPEETTVSGLVIPDTAKEKPQQGEVLAVG
                     PGRRAENSGEVIPIDLKVGDTVVYSKYGGTEITVDGEDLLILTGRDVLAKVQK"
     gene            complement(901..1914)
                     /gene="tsaD"
                     /locus_tag="VIJ44_06820"
     CDS             complement(901..1914)
                     /gene="tsaD"
                     /locus_tag="VIJ44_06820"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEY5165644.1"
                     /translation="MSIQANAPILGIETSCDETAAAVVAGGRFIQSSIVSSQVDLHAR
                     FGGVVPEIASRAHVDLVGPVIAEALGEATIDLDGLAAVAACRGPGLAGALLVGVSAAK
                     ALALVTGLPYVAVNHLEAHLYAAWLEEPDLEPPLAVLIVSGGHTLVVIMEGHGRYRVE
                     GQTVDDAAGEAFDKVARHLGLGYPGGPAIDGLAAHGDPEAIRFPRAMPGEPDLSFAGL
                     KTAVVTHLRRHPDAPVADVAASFQAAVVDQLADKLLAAADAAAAPTLVLGGGVAANSA
                     LRVRVAAAAAATGRRVFLPPPELCTDNAAMIAAAAWFRLAADGPTPLDDGVDPTLGLP
                     AVG"
     gene            complement(1911..2456)
                     /gene="rimI"
                     /locus_tag="VIJ44_06825"
     CDS             complement(1911..2456)
                     /gene="rimI"
                     /locus_tag="VIJ44_06825"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEY5165645.1"
                     /translation="MTVTAPLVVYVLPMRRRHLRSVLRIEALVYPRPWSHSLFLSELA
                     LRGSRAYFVARVGRDVVGYAGLMLSGDDAHITTIAVDPTHQRHKIGTRLLLVLAREAL
                     ARGAHNMTLEVRLSNHSAQDLYRRFGFGPVGVRKNYYQETNEDALVMWVHETDTSDYA
                     ALLAALERGIPGETLYDERRW"
     gene            complement(2453..>3009)
                     /gene="tsaB"
                     /locus_tag="VIJ44_06830"
     CDS             complement(2453..>3009)
                     /gene="tsaB"
                     /locus_tag="VIJ44_06830"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:NF013012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEY5165646.1"
                     /translation="CEQTQIELAQLAAVAVGIGPGLFTGLRVGVTTAKIMAQALRIPV
                     VAIPSPDLVAYPMRHSRRLLVVVLDARRHEVYSAQYRPVPGGVQRVSDYTVGSAHDLV
                     AELAAGGEEVLLAGDGVDANRDAFAALDRVEHAGPAFEAPSATALVELASGRVEREEF
                     VSPHDVRALYLRASDAELAWGQAR"
BASE COUNT          462 a         1001 c         1140 g          406 t
ORIGIN      
        1 cggcaccttc cgggtgttcg acggggtgcg gtactcggtg ccgggtgact gggcgacggt
       61 cgaggacgac gagacgatca cgctgctcgg acgaggctcg gtgtgcatca actcgggcgg
      121 cgagaagatc taccccgaag aggtggagga ggcgatcaag cgggacccga cggtggccga
      181 ctgcgtcgcc gtgggtgtcc ccgacgagcg gtggggcgag gcggtcaccg cggtggtggc
      241 agcggagccg ggcgccacgg tcgacccgga acgcctcaag gccgcggggg ctgcgctggc
      301 ccggttcaag cggcctcgcc acatcgtggt cgtggagcag atccgccgag gcccgaacgg
      361 caaggtcgac tacggctggg cccgctcgac cgccgcggaa cgggtggcca agaagtcctg
      421 acgcgccgcg acccccgaac gagccggggg ccgcgtgcgg cgaacgccag gtactacttc
      481 tggaccttgg ccaagacgtc gcggccggtc aggatcagca ggtcctcacc gtcgacggtg
      541 atctcggtcc cgccgtactt ggagtacacg acggtgtcac cgaccttcag gtcgatgggg
      601 atcacctcgc cggagttctc ggctcgccgg ccgggaccga ccgcgagcac ctcgccctgc
      661 tggggcttct ctttggcggt gtcgggaatg acgagcccgc tgaccgtggt ctcttcgggc
      721 tcgttcgacc ggacaacaat gcggtcctcg agcggctgca acttcatcgg gggcgcctcc
      781 tggggcgtcg aactgtctgc ggcaattagc actcggcagg tgtgagtgct agcgatcaac
      841 ataccgtgcc cggcgggggg cggcaagccc gcccggacgg ggtcgacggg gcggggccgg
      901 tcagccgacg gccgggagtc ccagcgtggg gtcgaccccg tcgtcgaggg gggtcggacc
      961 gtcggcggcg aggcgaaacc acgccgcggc ggcgatcatg gccgcgttgt cggtgcagag
     1021 ctccggtggg gggagaaaga ctcgccggcc ggtggcggcg gccgcggcgg ccactcggac
     1081 ccgcagcgcc gagttggcgg cgacgccgcc ccccaagacg agggtcggcg cggcagcggc
     1141 gtccgccgcc gcgaggagct tgtccgcgag ctggtcgacc accgcggcct ggaacgacgc
     1201 cgcgacgtcg gcgaccggcg cgtcgggatg gcggcgaagg tgggtgacca ccgcggtctt
     1261 gagaccggcg aaggacaggt caggttctcc gggcatcgcg cgcgggaacc ggatggcctc
     1321 ggggtcgccg tgggcggcga gcccgtcgat ggcggggccg cccggatagc cgaggccgag
     1381 atggcgggcc accttgtcga aggcctcccc ggcagcgtcg tcgacggtct gaccctcgac
     1441 gcggtagcgc ccgtggcctt ccatgatgac gacgagggtg tgaccacccg agacgatgag
     1501 aaccgcgagt ggcggctcga ggtcgggttc ctcgagccac gcggcgtaga ggtgcgcctc
     1561 aaggtggttc acggcgacgt acggcaggcc agtgacgaga gcgagtgcct tcgcggcgct
     1621 gacaccgacg agtaacgcgc cggcgagtcc cggaccgcga cacgcggcga ccgccgccaa
     1681 cccgtcgagg tcgatggtgg cctcgccgag cgcttccgcg atcaccgggc cgaccaggtc
     1741 gacatgcgcg cggctggcga tctcgggaac gaccccaccg aaccgggcgt gcaggtcgac
     1801 ttgactcgag acgatcgagg attggatgaa ccggccgccg gcgacgacgg ccgcggcggt
     1861 ctcgtcacac gaggtctcga tccccaagat cggggcgttc gcctggatgc tcaccagcgg
     1921 cgctcgtcat acagcgtctc gcccgggatc ccgcgttcga gcgcggcgag aagcgccgcg
     1981 tagtcgctgg tgtcggtctc gtgcacccac atcacgaggg catcctcgtt ggtctcttgg
     2041 tagtagttct ttcgcacccc gaccggcccg aacccgaacc ggcggtacag gtcctgggcg
     2101 gaatggttcg agaggcggac ctcgagggtc atgttgtgcg caccgcgggc cagcgcctcc
     2161 cgggcgagga cgaggagcag acgggtgccg atcttgtggc gctggtgggt cgggtccacc
     2221 gcgatggtcg tgatgtgggc gtcgtcgcca ctgagcatga gcccggcgta cccgacgacg
     2281 tctcgtccca cccgggcgac gaaataggcc cgcgacccgc gcaacgcgag ctcggacagg
     2341 aacagcgaat gactccaggg gcgggggtag acgagggcct cgattcgaag caccgagcgc
     2401 agatgccgtc gccgcatggg gaggacgtag acgaccaggg gtgcggtcac agtcaccggg
     2461 cctgccccca agccagctcg gcgtcactcg cccgcaggta gagggcccgc acgtcgtgag
     2521 gcgagacgaa ctcttcgcgt tcgacgcgtc cgcttgccag ctcgacgagg gcggtagcgc
     2581 tcggagcctc gaacgccggc cccgcatgct ccacccggtc gagggccgcg aacgcgtcgc
     2641 ggttggcgtc gaccccgtca ccggcgagga ggacttcctc gcccccggcc gcgagctcgg
     2701 ccaccagatc gtgggcggag cccaccgtgt agtccgagac ccgctgaacg ccgcccggga
     2761 ccggccggta ctgcgccgag tagacctcgt ggcggcgggc gtcgagcacg accacgagca
     2821 ggcggcggct gtgccgcatc gggtacgcga cgaggtcggg gctcggtatc gccacgacag
     2881 ggatccgcaa ggcttgggcc atgatcttcg cggtcgtcac gccgacccgc agaccggtga
     2941 agagaccggg tccgatcccg acggccaccg cggcgagctg ggcgagctcg atctgggtct
     3001 gctcgcaca
//
LOCUS       DATCCH010000358         2299 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_538616, whole genome shotgun sequence.
ACCESSION   DATCCH010000358 DATCCH010000000
VERSION     DATCCH010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538616"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="VIJ44_06835"
     CDS             <1..341
                     /locus_tag="VIJ44_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019068445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEY5165647.1"
                     /translation="DLDDVVPRLAAEAAARSGRPPPPPRAAIDHDRFGSGYGAVTDAT
                     VEAIELMGRLEGIVLDPVYTGKAMAGLLAAVRDGRIGRDETVVFWHTGGAPALFADRY
                     ATTFGPAIGA"
     gene            551..1144
                     /locus_tag="VIJ44_06840"
     CDS             551..1144
                     /locus_tag="VIJ44_06840"
                     /inference="COORDINATES: protein motif:HMM:NF022169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2510 domain-containing protein"
                     /protein_id="HEY5165648.1"
                     /translation="MSTTGRGVLYEERIGPGPRAWLWLPVIVAITSLAIAPVIVPIAV
                     VAWIVNILRYRGAVVRIDGDYAWVNRRWLRLASLDVDTLGRASNTWPWRAFNPRYLGA
                     NPIWTSDSVGVRGVDGGKKYWLSVGTNRRDELVGALRDAIPKARERAEQGAAISPYGL
                     PPPGWHPDPWEPTSLRWWDGQQWTGYTHPGPTGKVST"
     gene            1141..1944
                     /locus_tag="VIJ44_06845"
     CDS             1141..1944
                     /locus_tag="VIJ44_06845"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEY5165649.1"
                     /translation="MIPMADENPVRRIPWCTLALITACVVVYFAVQPGRHISFAWNGI
                     AETVNTQDLRFVVANAAIPCELVKGRPLTEQEFRQTFDNGDTNACTSASGPAHSPGKN
                     VYLAALLTLFLHASIPHLFGNMLFLWVFGNNIEDRKGWWRFLLLYLVGGAVATASQVL
                     IDPNSTVPIIGASGAIAAVMGAYLVCYPKARIKSMIFFGPILLRKVTAAWLLILWFVL
                     QFQYVISDAGVAWGAHVGGFTFGALVGLWWRLRERRSTLAPATPVPVPA"
     gene            1957..2050
                     /locus_tag="VIJ44_06850"
     tRNA            1957..2050
                     /locus_tag="VIJ44_06850"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1991..1993,aa:Sec,seq:tca)
     gene            complement(2113..>2299)
                     /locus_tag="VIJ44_06855"
     CDS             complement(2113..>2299)
                     /locus_tag="VIJ44_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEY5165650.1"
                     /translation="ERERQILALRFGLDRGEPRTLEEVGEYFNLTRERIRQIEARAMS
                     KLRHPSADTGARDLLAV"
BASE COUNT          330 a          802 c          802 g          365 t
ORIGIN      
        1 ccgatctcga cgacgtcgtc ccccggctgg ccgccgaggc cgccgcccgg agcggtcggc
       61 cgccgccgcc gccccgagcc gccatcgacc atgaccgctt cgggtcgggc tacggcgccg
      121 tcaccgacgc cacggtggaa gccatcgaac tcatggggcg cctcgaaggc atcgtcctcg
      181 accccgtcta cacgggcaag gccatggccg ggctcctcgc ggcggtgcga gacggtcgga
      241 tcgggcgtga cgagacggtg gtgttctggc acaccggggg cgccccggcg ctgttcgctg
      301 accggtacgc gaccacgttc gggccggcga tcggggcgtg acgcgcctcg ggcccgccga
      361 agcgggcccg agtgtcgacg tcggtgggat caggcttcgc ggagcttgcg cgcgaccgcg
      421 acaccgacca ccacgaggac cagcaacacg accagcttct tcatcggtgc tccccggatc
      481 ggtggaagga accctgaggt taccgcggtc ccggggcccc gcccgtcgag cctctacgct
      541 cccgcccgag gtgagcacga ccgggcgcgg agtcctctac gaagaacgga tcgggccggg
      601 gccgcgcgcg tggttgtggc ttcccgtcat cgtcgccatc acgtcgctgg cgatcgcccc
      661 ggtgatcgtg ccgatcgccg tcgtggcctg gatcgtgaac atcctgcggt accgcggcgc
      721 ggtcgtgcgc atcgacggtg actacgcctg ggtgaaccga cgctggttgc gcctcgccag
      781 cctcgacgtg gacaccctgg ggcgggcgag caacacgtgg ccgtggcggg ccttcaatcc
      841 ccggtacctg ggcgcgaacc cgatctggac cagcgactcg gtgggcgtac gcggcgtcga
      901 cggcggcaag aagtactggc tgtcggtcgg aaccaaccgg cgcgacgagc tggtcggggc
      961 gctgcgcgac gccatcccca aggcgcggga acgggccgag caaggcgcgg cgatttctcc
     1021 gtacgggctg ccaccaccgg gttggcatcc cgacccgtgg gagccgacgt cgctgcgctg
     1081 gtgggacggg cagcagtgga ccggctacac gcacccgggc ccgacgggga aggtttcgac
     1141 atgatcccga tggccgacga gaaccccgtc cggcgaatcc cgtggtgcac cttggcgctg
     1201 atcaccgcct gcgtggtcgt ctacttcgcg gtgcagccgg gacgccatat cagtttcgct
     1261 tggaacggca tcgccgagac cgtcaacacc caagacctcc ggttcgtggt cgccaacgcc
     1321 gccatcccct gcgagctggt caagggtcgg cccctcaccg agcaagagtt ccggcagacc
     1381 ttcgacaacg gcgacaccaa cgcctgcacc agcgccagcg gcccggccca ctcgccgggt
     1441 aagaacgtgt acctcgcggc cttgctcacg ctgttcctgc acgccagcat cccgcacctg
     1501 ttcggcaaca tgctgttcct ctgggtgttc ggcaacaaca tcgaggaccg caaaggctgg
     1561 tggcgtttcc tgttgctcta tctcgtcggt ggggccgtcg cgaccgcgtc tcaagtcctg
     1621 atcgacccga attcgacggt gccgatcatc ggggcgtcgg gggcgatcgc ggcggtgatg
     1681 ggcgcctatc tcgtctgcta cccgaaagcc cgcatcaagt cgatgatctt cttcgggccg
     1741 attctgctgc gaaaggtcac cgcggcgtgg ctgctgatcc tgtggttcgt actccagttc
     1801 cagtacgtga tctccgacgc gggggtcgcc tggggcgccc acgtcggcgg gttcacgttc
     1861 ggggcgctcg tcggtctgtg gtggcggctc cgagagcgac gctcgacgct cgctccggcg
     1921 acaccggtcc cggtcccggc ctgatacaac ccgtacggag gtgaatgggg cctggtggcc
     1981 cccctcgtct tcaaaacgag tgggacgggc aatccctgtc cggtgggttc gattcctgcc
     2041 gcctccgcca cagtgcaacg cgggcgaccc gcgcccgtcg ccggacggag cccgcgagtg
     2101 gcggagcggc gcttagaccg cgaggaggtc gcgggcgccg gtgtcggccg acgggtggcg
     2161 gagcttcgac atcgcccggg cctcgatctg gcggatccgt tcccgggtga ggttgaagta
     2221 ctccccgact tcttcgaggg tgcgcggctc gccccggtcg agaccgaacc gcaaggcgag
     2281 gatctggcgc tcgcgctcg
//
LOCUS       DATCCH010000359         2498 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_538718, whole genome shotgun sequence.
ACCESSION   DATCCH010000359 DATCCH010000000
VERSION     DATCCH010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538718"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /gene="grpE"
                     /locus_tag="VIJ44_06860"
     CDS             <1..508
                     /gene="grpE"
                     /locus_tag="VIJ44_06860"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEY5165651.1"
                     /translation="ERAAEETAAEDGSAGEAEADLDAVLATLAKERDELRELAQRVQA
                     DFENYRKRMLRDQTDAIARANESLIEELLPVVDSFELALGQLADTDEQVRKGVELVFA
                     GLVDVLDKAGLERIDSTGVPFDPNVHEAVMQEGDHGDPVVADTVRTGYRLKGRVLRPA
                     MVKVARKD"
     gene            514..1665
                     /gene="dnaJ"
                     /locus_tag="VIJ44_06865"
     CDS             514..1665
                     /gene="dnaJ"
                     /locus_tag="VIJ44_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014743110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEY5165652.1"
                     /translation="MAPQREWFEKDYYAVLGIEPGASDREIQRAYRSLAKANHPDANP
                     GDHAAEERFKEISAANDVLGDSDKRKEYDQVREMVASGAGPGGFGGAPGGFGPGFEGA
                     TFHFDDGGGLGDLLGNLFGARSGPRRRGRAPTGPVHGADLETELHLDFADAVLGVTTT
                     VGVTSDAPCSVCGGSGAEPGTFPQPCPTCGGTGAVAVDQGPFSFSQVCPTCGGRGEVI
                     EHRCKKCRGTGVERRRREVKVRIPAAVRDGQRIRVKGRGAAGRNGGPPGDLYVVVHVG
                     SDDRFGRQGKINLTVKVPITFSEATLGAQVKVPTLDAPVTVKVPAGTQSGKTVRIRGR
                     GIQPSKGDPGDLLVTFEVVVPTELTEEQRAAIAALADQLPANPREHLGV"
     gene            1674..2060
                     /locus_tag="VIJ44_06870"
     CDS             1674..2060
                     /locus_tag="VIJ44_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY5165653.1"
                     /translation="MDDRERALYIISVAAELAGVHAQTLRIYERKGLIEPSRTQGGSR
                     RYSDRDIALLHRIQELTTDGVSLAGVRKVLELEADLDDARDRIADLETELDRQRQAQR
                     VEVEAVHRQYRRTLVPVSQAVVRVRR"
     gene            complement(2070..>2498)
                     /locus_tag="VIJ44_06875"
     CDS             complement(2070..>2498)
                     /locus_tag="VIJ44_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165654.1"
                     /translation="AGFSLTYATTWRIQRVNPGQGIRFVVQSRHGSADQLTTVSVTAG
                     TQKGPLPTAADLARTVIDGLRPQFPGIMLQTNGTTALAGGPAEMLTFADPGTNPPTTI
                     EQVVGRTADGRPLTVTVQVPDPRYAPGDADVHAFLASITS"
BASE COUNT          409 a          874 c          884 g          331 t
ORIGIN      
        1 cgaacgggcc gctgaggaaa ccgcggctga ggacggctcc gccggcgagg ccgaggcgga
       61 cctcgacgcg gtgctcgcca cgctcgccaa ggagcgcgac gagctgcggg agctggccca
      121 gcgggtccag gccgacttcg agaactaccg gaagcggatg ctccgcgacc agaccgacgc
      181 catcgcccgc gccaacgaga gcctgatcga agagctcctc cccgtcgtgg acagcttcga
      241 gctcgccctc ggccagctcg ctgacaccga cgagcaggtc cgcaagggtg tcgagctcgt
      301 ctttgccggc ctcgtcgacg tcttggacaa ggcgggtctt gaacgtatcg acagcaccgg
      361 agtcccgttc gatccgaacg tccacgaggc cgtcatgcaa gaaggcgacc atggtgaccc
      421 cgtcgtcgcc gacacggtcc gcaccggcta ccgactgaag ggtcgagtgc tgcgcccggc
      481 gatggtgaag gtcgcccgca aggactgaca gcgatggcac cccagcgcga gtggttcgag
      541 aaggactact acgccgtgct cggcatcgag cccggcgcga gcgatcgtga gatccaacgc
      601 gcctatcgat ccctggccaa ggcgaaccac cccgacgcca atcccgggga ccacgcggcc
      661 gaggagcgct tcaaggagat ctcggccgcg aacgacgtgc tcggcgactc cgacaagcgc
      721 aaggaatacg accaggtccg cgagatggtc gcgtccgggg ccggacccgg cggcttcggc
      781 ggtgcaccgg gcggattcgg gcccggcttc gaaggcgcaa cgtttcactt cgacgacggc
      841 ggcgggctcg gcgatctcct cggcaacctg ttcggcgccc gcagcggacc acgtcgacgc
      901 gggcgcgccc ccaccgggcc ggtccacggc gcggacctcg agaccgagct ccacctcgac
      961 tttgctgacg ccgtcctcgg tgtgaccacc accgtgggtg tcacgtccga cgcgccctgc
     1021 tcggtctgcg gcggatccgg cgccgagccg gggaccttcc cgcagccgtg cccgacctgt
     1081 ggagggaccg gagccgtcgc ggtcgatcag ggcccgttct cgttctcaca ggtctgtccc
     1141 acctgcgggg ggcgcggcga ggtgatcgag caccggtgca agaagtgtcg agggaccggc
     1201 gtcgagcgcc gccgccgcga ggtcaaggtc cggatccccg ccgcggtccg cgacggtcaa
     1261 cggatccggg tcaagggccg aggcgccgcg ggccgcaacg ggggtccgcc cggcgacctg
     1321 tacgtcgtcg tgcacgtcgg gtccgacgac cgcttcgggc gacaaggcaa aatcaacctc
     1381 acggtcaagg tcccgatcac cttcagcgag gcgacactcg gcgcgcaggt caaggtgccc
     1441 acgctcgacg cgccggtcac cgtcaaggtc ccggccggca cccagagcgg caaaaccgtc
     1501 cggatccggg gccgcggaat ccagccgtcg aagggcgatc cgggggatct gctcgtcacc
     1561 ttcgaggtcg tggtgcccac cgagctcacc gaagaacagc gggcggcgat cgccgccctc
     1621 gcggaccagc tgccagcgaa cccgcgggag cacctcggcg tctaggggga gccatggacg
     1681 atagagaacg ggcgctgtac atcatctcgg tcgccgcgga gctcgccggc gtccacgcgc
     1741 agaccttgcg gatctacgaa cgcaagggct tgatcgagcc gagccgcacc caaggtggca
     1801 gtcgccgcta ctcggatcgc gacatcgcgt tgctgcaccg aatccaggag ctcaccaccg
     1861 acggcgtgag cctggccggg gtgcgcaagg tgctcgagct cgaagccgac ctcgacgatg
     1921 cccgtgaccg gatcgccgac ctcgaaaccg agctcgaccg ccagcgccaa gcgcagcgcg
     1981 tggaggtcga agcggtccac cgccagtatc gccgcacgct cgtaccggtg agccaggcag
     2041 tggtccgagt ccgccgctga gcggcggtgt caggacgtga tcgaggcgag gaacgcgtgc
     2101 acgtcggcgt cgcccggcgc gtaccgagga tcgggaacct gcacggtcac ggtcaggggc
     2161 cgaccgtcgg cggtgcgccc gacgacctgt tcgatcgtgg tcggcggatt cgtgcccggg
     2221 tccgcgaacg tcagcatctc cgccggcccg ccggcgagcg cggtggttcc gttcgtctgc
     2281 agcatgatcc ccgggaactg gggccggagg ccgtcgatga ccgtgcgggc gaggtcggcg
     2341 gcggtcggca gcgggccctt ttgggtgccg gcggtcaccg acaccgtcgt cagctgatcg
     2401 gcggacccgt gccgcgactg cacgacgaag cggatgccct gccccgggtt cactcgctgg
     2461 atcctccagg tcgtcgcgta ggtgagcgag aagccggc
//
LOCUS       DATCCH010000360         4893 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_123975, whole genome shotgun sequence.
ACCESSION   DATCCH010000360 DATCCH010000000
VERSION     DATCCH010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4893
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123975"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VIJ44_06880"
     CDS             complement(<1..407)
                     /locus_tag="VIJ44_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEY5165655.1"
                     /translation="MDQGRLDAATLERRIGDDEIDTVLVAFTDLHGRLVGKRVTGHFF
                     SDQVLRDGIEVCNYLLAVDVDMTPLPGYQFANWDQGYGDVLCRPDLATLREVPWLEKT
                     ALVLCDLVDVNTGDPVEVSPRQVLRRQIERAAA"
     gene            complement(484..1269)
                     /locus_tag="VIJ44_06885"
     CDS             complement(484..1269)
                     /locus_tag="VIJ44_06885"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEY5165656.1"
                     /translation="MSTPRRLEDKVALITGAGNGMGRAAALLFASEGARIVVADAVEE
                     GGALTVDSVRDAGGDATFVQVDVSEADQVSSMVDAAMKRYGALHVLYNNAGIFPADDA
                     GVTDTPEATWDRVMDVNLKGVWLGCRFGVPAMLDSGGGSIVNVSSLVALMGSATPQVA
                     YTTSKGGVLALTRELAIEYSRRGIRANALCPGPIETPLLAELLDDPEWARRRLVHIPM
                     GRPGRAEEVARAALFLASDDSSFMTGASLVVDGGISAAYVTPE"
     gene            1365..2273
                     /locus_tag="VIJ44_06890"
     CDS             1365..2273
                     /locus_tag="VIJ44_06890"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEY5165657.1"
                     /translation="MNAGPGVSITISGLSRLFGADLSAVIEVARVADEAGVHQLVLPD
                     HLAIGPRTDRYPFGTFPYPPSEPWLEPLTTLAAIAGATERVRLGTGVLIAPLRPALLL
                     AKTAATLDVLSGGRLDLGVGTGWQREEFTDAGQPFQGRTSRLDDTVRACRALWNQPPP
                     VSFTSPTVTFSELWCEPRPHQAGGIPVWFGGGPTDATARRIAALGDGWLPVGVMPFDE
                     LSTGIEMIRAAYREVGRDPVGLGVRAGIAVSTHDNGRLDLTQTLADVPRLAAAGVTLI
                     SLSLGRFLRDRDDVAPFLREVAAAFP"
     gene            2297..3775
                     /locus_tag="VIJ44_06895"
     CDS             2297..3775
                     /locus_tag="VIJ44_06895"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY5165658.1"
                     /translation="MLPPIRRVLSVVALTAFLISVVPTGIASASVASAVPHSTLRWTA
                     CSGGVECARLKVPLDDTVVGGPTISLAIARVRARDPQHRIGSLLVNPGGPGASGIQYL
                     RDSFQALPGTIRDRFDIVSFDPRGTGASAPVECTPNLDSLYNLEFAPRDDAARQALVA
                     GVQAFIAKCQANAGAELPYVSTERAARDMDRIRAALGDSRLTYLGYSYGTYLGSLYAD
                     RYPTHVRAMVFDGAVDPALDAAAVQIQQAQGFEHSLDLFLQSCAADTTCSFHRGGNTA
                     AAYDALRARVGANPIPAPTAGKGRTLNATEFDIGVTFLLYSGQHAWPDLAHFLDNADS
                     GDGSELLDQADASNMRNPDGTYDTLQDAFLAIGCLDGPDVGGLTGLRAIEEQAAQVAP
                     RLGRSVVNNSLACAVWPVPVQTAPVPHAVGAPPIVVVGNTDDPATPLAWATGLSHELQ
                     SAVLLTVRSTQHTAYASGNSCVDTAINRYLIDVRPPAAGTRC"
     gene            3846..>4893
                     /locus_tag="VIJ44_06900"
     CDS             3846..>4893
                     /locus_tag="VIJ44_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007454158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY5165659.1"
                     /translation="MDLRLSASDKAFRAELQSWLRRELPQLPPAPARDDWPARVRYDT
                     DWQRRLFDAGYAGMSWPQAYGGRDASPTEQLIFLEETTRAEAPYVGVNFVGNLHAGPT
                     LIAEGSEAQKAEHLPRILRGDEVWCQCFSEPEAGSDLAGLRTRAVRDGDDYVVTGQKI
                     WCSFGQVGEFGELLVRTDPEASKHRGISWLILPMDRPGIDVRPIETLLGSSEFCEVFL
                     DEVRVPVANRVGAENDGWRVTNVTLSFERGTAFVSEMVDARRLAEELRPIVDDPAERR
                     ELGHVVAALDALWALTQRNVTQAGHGTPGPGALMFKLAYSEARDRLGELSMRVLGRGA
                     LDVHDYRVEERLRTL"
BASE COUNT          709 a         1842 c         1593 g          749 t
ORIGIN      
        1 aaggccgccg cccgttcgat ctgccggcgc agcacctggc ggggggagac ctcgaccgga
       61 tctccggtgt tgacgtcgac caagtcgcac aggacgaggg ctgtcttctc gagccacggc
      121 acttcccgaa gcgtggccag gtcgggccga cagaggacgt cgccgtaacc ctgatcccag
      181 ttggcgaact ggtagcccgg cagtggggtc atgtcgacat cgaccgcgag gaggtagttg
      241 cagacctcga tgccgtcacg cagcacctgg tcggaaaaga agtggccggt cacccgtttg
      301 ccgaccaggc ggccgtgcag gtcggtgaac gccacgagca ccgtatcgat ctcatcgtcg
      361 ccgatgcggc gctccagcgt cgcggcgtcg agtcgccctt gatccatgcg tggccctcct
      421 gactggccgt tcatcctctc acgcccgcgt tggggcgcga ccgggagtgg acggccgtga
      481 cgcctactcc ggggtcacgt acgccgcact gataccaccg tcgaccacca gcgacgcgcc
      541 agtcatgaac gacgaatcgt cggaggcgag gaacaacgcc gcccgcgcga cctcctcggc
      601 tcgccccggc cgtcccatcg ggatgtggac cagccgtcga cgagcccact ctggatcgtc
      661 gagtagctcg gcgagcaacg gtgtctcgat cggtcccgga cacaacgcgt tggcgcggat
      721 cccacgccgc gagtactcga tcgccagctc tcgagtcagg gcgagcacgc cacccttgct
      781 cgtggtgtag gccacctggg gcgttgctga gcccatcagc gccacgagtg acgacacgtt
      841 cacgattgac ccgccacccg agtcgagcat ggcggggaca ccgaaccggc agcccagcca
      901 cacccccttg aggttcacgt ccatcacccg atcccacgta gcctcgggcg tgtcggtcac
      961 cccggcgtcg tcagccggga agatcccggc gttgttgtag agcacatgca gcgccccgta
     1021 tcgcttcatc gccgcgtcga ccatcgacga cacctggtcc gcctccgaga cgtcgacctg
     1081 cacgaaggtc gcgtcgccgc ccgcgtcgcg caccgagtcg acggtcagcg caccgccctc
     1141 ctccaccgcg tcggcgacga cgatccgggc accctccgac gcgaacagca gcgccgcggc
     1201 tcgccccatg ccgttcccgg ccccggtgat gagggcgacc ttgtcttcta ggcggcgcgg
     1261 tgtcgacatc cccgtatgct gccccgcgtg gggcgtccgg gccatctgtc gaaccgccgg
     1321 tccgcgcccg gctggcctcc gcgacccacg accagaggct cgaggtgaac gccggcccgg
     1381 gtgtgtcgat cacgatctcg gggctgtcgc ggctgttcgg cgccgacctg agcgccgtga
     1441 tcgaggtggc ccgcgtcgct gacgaagccg gtgtccacca gctcgtcctc ccggatcatc
     1501 tcgccatcgg gcctcgaacc gaccggtacc cgttcggcac cttcccgtac ccgccatcgg
     1561 agccgtggct cgagccgttg acgacgctgg ccgcgatcgc gggcgccacc gagcgggtcc
     1621 gtctcggcac cggcgtgctc atcgcgccgc tccggccggc gctgctcctc gccaagaccg
     1681 ccgcgacgct cgacgtgctc agcggcggac gactcgacct cggcgtcggc accggatggc
     1741 agcgcgaaga gttcaccgac gccggccagc cgttccaggg ccgaacctcg cgactcgacg
     1801 acaccgtccg ggcctgccgg gccctctgga accagccccc gccggtgtcg ttcacgtccc
     1861 cgacggtcac gttcagcgag ctgtggtgcg agccgcgtcc ccatcaggcg gggggcatcc
     1921 ccgtctggtt cggcggcggt ccgacggatg cgactgcccg ccgcatcgcc gcgctcggtg
     1981 acggctggct cccggtgggg gtgatgccct tcgatgagct gagcacaggc atcgagatga
     2041 tccgggccgc gtaccgcgag gtcggtcgcg acccggtcgg gctcggcgtc cgcgccggca
     2101 ttgcggtgag cacccacgac aacgggcgcc tcgacctgac tcagacgttg gcggacgttc
     2161 cgcgtctcgc cgcggccggc gtcaccctga tctcgctctc gctcggccga ttcctccgag
     2221 atcgcgatga tgtcgcgcca tttctgagag aggtcgccgc ggcgtttccg taggcctcga
     2281 cgcgtacgct cgtccggtgc tgcccccgat ccggcgcgtg ctcagcgtcg tcgcgctcac
     2341 cgcgttcctc atcagtgttg tgcccaccgg catcgcgtcc gcgtcggtgg cctccgcggt
     2401 tccccattcc accctccggt ggacggcgtg cagcgggggc gtggagtgcg cccggctcaa
     2461 ggtcccgctc gacgacaccg tcgtgggtgg gccgacgatc agcctcgcga tcgcccgcgt
     2521 gcgggcccgg gatccgcagc accgcatcgg atcgctcctc gtgaacccgg gtggcccggg
     2581 cgcgtccggg atccagtacc tgcgcgactc cttccaggcc ctgcccggga ccatccgcga
     2641 ccggttcgac atcgtgagct tcgacccacg cgggacgggc gcgagcgcac cggtggaatg
     2701 caccccaaac ctcgattcgc tgtacaacct cgaattcgcg ccccgggacg acgcggctcg
     2761 ccaggcactc gtggccggtg tgcaagcctt catcgcgaag tgtcaagcga acgccggcgc
     2821 cgagctcccc tacgtgtcga ccgagcgggc ggcacgcgac atggaccgga tccgggccgc
     2881 gctcggcgac tcgcgcctca cctacctcgg atattcgtac ggcacctacc tcgggtctct
     2941 ctacgccgac cggtacccaa cccacgtccg ggcgatggtg ttcgacggcg ccgttgaccc
     3001 cgcgttggac gccgccgcgg tgcagatcca gcaggcccag ggcttcgagc actcgctcga
     3061 cctgttcctg cagtcctgcg ccgcggacac cacgtgctcg ttccatcgtg gcggtaacac
     3121 cgccgcggcg tacgacgccc tgcgggcacg cgtcggcgcg aacccgattc ctgcccccac
     3181 tgccggcaag gggcgaacgc tcaacgccac cgagttcgac atcggggtca cctttctcct
     3241 gtactccggc cagcacgcct ggcccgatct cgcccacttc ctcgacaacg cggactctgg
     3301 cgacggctcc gaactgctgg accaggccga cgcctcgaac atgcgcaacc ccgacggcac
     3361 ctacgacacc ctccaggacg ccttcctcgc catcggctgc ctcgacggcc ccgacgtcgg
     3421 ggggctcacg ggtctccgcg cgatcgagga gcaggcggcg caggtcgcgc cccgtctcgg
     3481 acgttcggtc gtcaacaaca gcctggcgtg cgccgtgtgg ccggtcccgg tgcagaccgc
     3541 gccggtgccg cacgccgtcg gtgccccacc catcgtggtc gtgggcaaca ccgatgaccc
     3601 cgcgaccccg ctggcctggg cgaccgggct ttcccacgag ctgcagtcag ccgtgctgct
     3661 caccgtccgc agcacgcagc acaccgcgta cgcatcgggt aactcgtgtg tcgacaccgc
     3721 gatcaaccgg tacctcatcg acgttcgccc tcccgcggct gggacacgct gctgaccgcg
     3781 agcgcgaacc cccaactccg gccgatctga cgcttcgtca ggaccgggcg tacgctcccg
     3841 cgccgatgga cctgcgactc tcggcatccg acaaagcgtt ccgcgccgag ctgcagtcct
     3901 ggctccgccg cgaactcccg cagcttccgc ccgcaccggc ccgggacgac tggcccgccc
     3961 gggttcgcta tgacaccgac tggcagcgac gcctcttcga cgccggatac gccgggatga
     4021 gctggcccca ggcctacggc ggccgtgacg cgtcgcccac cgagcagctg atcttcctgg
     4081 aggagaccac ccgcgccgag gcgccgtacg tcggtgtcaa cttcgtcggc aacctgcacg
     4141 caggcccgac cttgatcgcg gaaggcagcg aagcccagaa ggccgagcac ctcccgcgaa
     4201 tccttcgcgg ggacgaggtc tggtgccagt gcttctcgga gccggaagcc ggctcggacc
     4261 tcgccggtct tcgcacccgc gccgtgcgcg acggcgacga ctatgtcgtc accggccaga
     4321 agatctggtg ctccttcggc caggtcggcg agttcgggga gctgctggtc cgcaccgacc
     4381 ccgaagcgtc gaagcaccgc gggatctcgt ggctcatcct cccgatggac cggccgggca
     4441 tcgacgtccg tcccatcgag acgctgctcg gatcctcgga gttctgtgag gtgttcctcg
     4501 acgaggtacg cgtcccggtc gccaaccggg tcggagcgga gaacgacggg tggcgagtta
     4561 cgaacgtcac tctgtccttc gagcggggga cggcgttcgt gagcgagatg gtcgacgccc
     4621 ggcgcctcgc cgaggagcta cgccccatcg ttgacgatcc cgccgagcgc cgagagctcg
     4681 gccatgtggt cgcggcgctg gatgccctct gggcgttgac gcaacgcaat gtcacccagg
     4741 ccggccacgg cacccccggg ccgggggcgc tcatgttcaa gctcgcgtac tccgaagctc
     4801 gcgatcggct cggcgagctc tcgatgcggg tgctcgggcg cggcgccctc gacgtccacg
     4861 actaccgggt ggaggaacgc ctccgcaccc tgt
//
LOCUS       DATCCH010000361         2273 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_290023, whole genome shotgun sequence.
ACCESSION   DATCCH010000361 DATCCH010000000
VERSION     DATCCH010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290023"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1776
                     /locus_tag="VIJ44_06905"
     CDS             <1..1776
                     /locus_tag="VIJ44_06905"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEY5165660.1"
                     /translation="IRVLLIKLADRLHNMRTISSLAETKQRRIARETLDIYAPLAHRL
                     GIQDLKWQLEDLAFEVLHPKRYAEIEQMVASRAPARDEELRHVLAELRDRLADLHIEA
                     QVQGRPKHYWSIYEKMVVKGKEFDDVQDLVGVRVIVDSVKDCYGALGSIHALWSPVQG
                     RFKDYIAMPKFNLYQSLHTTVVGPDGRPVEVQIRTKEMHRRAEFGIAAHWGYKERSPA
                     EDLAWLQRMVDWQQDTDDPTEFMETLKVDLHYDEVFVFTPKGKVVTLPSGANPIDFAY
                     AIHTEVGHRCIGARVNGRLVPLDSTLSSGDTVEIFTSKVEGAGPSLDWMQFVHTPRAR
                     AKIRQWFSRERRVDAMDTGREELLKALRKEGLPTQKLTQSVALKQVAAALHYTDLDTL
                     HAAIGEGHVSARAVAQRVQRELRGGEEQLPVTANRPPATSRPQRTRTVGVHVEGLDDV
                     MVRLSRCCTPVPGDEIMGFVTRGRGVSVHRADCANAAGLSTQTQRVIDVEWDHDETAT
                     FVVSVEIEALDRSRLLRDVAEVLAEHHVNILTSSSQTSSDRVARFRFDFELADPSHLD
                     SILQAIARVDSVYEAYRLLPGHARN"
     gene            1815..>2273
                     /locus_tag="VIJ44_06910"
     CDS             1815..>2273
                     /locus_tag="VIJ44_06910"
                     /inference="COORDINATES: protein motif:HMM:NF024785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEY5165661.1"
                     /translation="MAEAEYRAPVGTHDVLPPESSRWSALVTAFATRAERFGYGLVIT
                     PVMEHVEVFQRVGAATDVVRKEMYELTDRGDRRLALRPEGTAPVVRAYVQHHPTPPWK
                     VWYLAPHFRYERPQKGRYRQHWQVGVEVLGVDDPTVDVEVIELAHGFYRDD"
BASE COUNT          421 a          871 c          653 g          328 t
ORIGIN      
        1 attcgggtgc tgctcatcaa gctcgctgac cgtctccaca acatgcgcac catctcgtcg
       61 ctcgccgaga cgaagcagcg gcgaatcgcg cgcgagaccc tcgacatcta cgcccccctc
      121 gcgcaccggc tcgggatcca agacctcaag tggcagctcg aggatctcgc cttcgaggtc
      181 ctgcacccga agcgctacgc cgagatcgag cagatggtcg cgagccgggc ccccgcccgc
      241 gacgaggagc tccgacacgt cctcgccgag ctgcgggacc gcctcgccga tctgcacatc
      301 gaagcccagg ttcagggccg cccgaagcac tactggtcga tctacgagaa gatggtcgtc
      361 aagggcaaag agttcgacga cgtccaagac ctcgtcggcg tgcgcgtcat cgtcgactcg
      421 gtgaaggact gctacggcgc gctcggctcg atccacgcgt tgtggtcacc ggtccaaggc
      481 cggttcaagg actacatcgc catgcccaag ttcaacctct accagtcgct gcacaccacc
      541 gtcgtcgggc ccgacggacg accggtcgaa gtccagatcc gaaccaagga gatgcaccgc
      601 cgcgccgagt tcggcatcgc cgcccactgg ggttacaagg aacgctcccc ggctgaggat
      661 ctggcttggc tccaacgcat ggtcgactgg caacaagaca cggacgaccc aactgagttc
      721 atggagaccc tcaaagtcga tctccactac gacgaggtgt tcgtcttcac acccaaaggc
      781 aaagtcgtca ccctcccgtc gggcgcgaac cccatcgact tcgcctacgc catccacacc
      841 gaggtcgggc atcgttgcat cggcgcccgc gtcaacggcc ggctcgtccc tctcgactcc
      901 acactcagct ccggggacac cgtagagatc ttcaccagca aggtcgaagg cgccgggccg
      961 agcctcgact ggatgcagtt cgtccacacc ccccgcgccc gggccaagat ccgccagtgg
     1021 ttctcccgcg aacgtcgcgt cgacgccatg gacacgggcc gcgaagagct cctcaaggcc
     1081 ctccgcaagg agggcctccc cacccagaag ctcacccagt ccgtggcact gaagcaggtc
     1141 gccgccgcgc tccattacac cgacctcgac acgctccacg ccgccatcgg cgaaggtcac
     1201 gtctcagccc gcgccgtcgc gcaacgcgtg caacgcgagc tacgcggagg agaagagcag
     1261 ctcccggtca ccgccaaccg gccaccagcc acctcccgcc cgcaacgaac tcgcaccgtc
     1321 ggcgtccacg tcgagggcct cgacgacgtg atggtccgcc tctcgcgctg ctgcacccct
     1381 gtccccggcg acgagatcat gggcttcgtg acccgcggtc ggggcgtgtc ggtccatcgc
     1441 gccgactgcg ccaacgccgc cgggctctcc acccaaaccc aacgggtcat cgacgtcgaa
     1501 tgggaccacg acgagaccgc gaccttcgtc gtctcggtcg agatcgaagc cctcgaccgc
     1561 tcgcgactcc tccgcgacgt cgccgaggtg ctcgccgagc accacgtcaa catcctgacc
     1621 agcagctccc agacctcgag tgaccgcgtc gcgcgcttcc gattcgactt cgagctcgcc
     1681 gaccccagcc acctcgactc catcctgcaa gccatcgccc gggtcgactc ggtgtacgag
     1741 gcgtatcgct tgctcccggg acacgcacgg aactagtccg gggacgcgca acagcccctc
     1801 ggtagcatca tccaatggcc gaagccgaat accgcgcgcc ggtcggaacc cacgatgtcc
     1861 tcccacccga atcgtcgcgc tggagcgcac tcgtcacggc gttcgcgacc cgagccgaac
     1921 ggttcggcta cggcctcgtc atcaccccgg tcatggagca cgtcgaggtg ttccagcgcg
     1981 tcggcgccgc caccgacgtg gtccgaaaag agatgtacga gctcaccgac cgtggtgacc
     2041 gccgcctcgc cctccgaccc gaaggcaccg cccccgtcgt gcgggcctac gtgcagcacc
     2101 accccacacc cccgtggaag gtctggtacc tcgccccgca cttccgctac gaacgacccc
     2161 agaagggccg gtaccgccag cactggcagg tcggcgtcga ggtgctcggc gtcgacgacc
     2221 cgacggtcga cgtagaggtc atcgagctcg cgcacggctt ctaccgcgac gac
//
LOCUS       DATCCH010000362         2218 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_19818, whole genome shotgun sequence.
ACCESSION   DATCCH010000362 DATCCH010000000
VERSION     DATCCH010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19818"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="VIJ44_06915"
     CDS             complement(<1..348)
                     /locus_tag="VIJ44_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165662.1"
                     /translation="MSVIVALAPVSAGRVGEQAAKTVILVIVLLIGFRISGKREMSQF
                     TVYDLAMIIALSNAVQNAMTGGLGNFPIGLATSSTIVVTAWGISRLLARRPRLATEVL
                     GTPTLLVNDGRVLT"
     gene            complement(345..878)
                     /locus_tag="VIJ44_06920"
     CDS             complement(345..878)
                     /locus_tag="VIJ44_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HEY5165663.1"
                     /translation="MPLLAQVFDYPSVNGAATIAAKTAIIYAFLILGLRLLGKRELGQ
                     LSLYDLVMVIILGNAVQNAMINNDNTLGGGLVAATVLLVLNWTLNRVITRSRTAERVL
                     VGEPILIVHDGKPLRDRMRRQGITMDQLASALREHGLDDVSAVHLAILESDGTISVVP
                     SGSPMLKTRRHYRGLRL"
     gene            962..1423
                     /locus_tag="VIJ44_06925"
     CDS             962..1423
                     /locus_tag="VIJ44_06925"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HEY5165664.1"
                     /translation="MGTLLIVAGSTAAIYLFLITILRFVGRRQLAQLSALDLVVFLLL
                     GSAVETAMIHGDVSLAAGLVAAATLLILNRLLTLVFLHAPQLSHLVNGGPVLLVHDGA
                     VIEEHLRRVGLTHPDLDAALRSRGFASSDGVREAVLETDGSITVVGERGGP"
     gene            1576..>2218
                     /locus_tag="VIJ44_06930"
     CDS             1576..>2218
                     /locus_tag="VIJ44_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013159491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165665.1"
                     /translation="MTPTVVLVVSAFLASAVEMVEALTIVLALGVTRGWRSVTIGAAA
                     AVAALTLVVATLGPALTQVPISALRLVVGTVLLVFGLQWLRKAILRSAGVRALHDEDE
                     VFAREIDESRHADRRTVARLDWYAFTLSFKGVFLEGLEVAFIVVTFGSTEGHIGLAAA
                     GAGAALIVVGTIGAFVHRPLSRVPENSLKFAVGVMLSAFGMFWSAEGAGVSWPG"
BASE COUNT          314 a          724 c          785 g          395 t
ORIGIN      
        1 ggtgaggacg cgcccgtcgt tgacgagcag ggtcggggtt ccgagcacct ccgtcgcgag
       61 gcggggccgg cgggcgagga gccggctgat gccccaggcg gtcacgacga tggtgctcga
      121 cgtcgcgagc ccgatgggga agttgcccaa cccgcccgtc atggcgttct gcaccgcgtt
      181 cgacaacgcg atgatcatcg ccaggtcgta cacggtgaac tgggacatct cgcgcttccc
      241 gctgatccga aagccgatga ggagcacgat caccaagatg acggtcttgg ctgcctgctc
      301 gccgacacga cccgcgctca ccggtgccag cgcgacgatg acgctcacag ccgcagtcca
      361 cggtagtgac gacgggtctt cagcatcggc gatccactcg ggacgacgct gatcgtcccg
      421 tcgctctcca ggatggcgag atggacggcg ctgacgtcgt cgaggccgtg ctctcggagc
      481 gccgaggcga gctggtccat cgtgatccct tgtcgtcgca tgcggtctcg caacggcttg
      541 ccgtcgtgga cgatcaggat gggctcaccg acgaggaccc gctccgcggt ccgagatcgc
      601 gtgatcactc ggtttagcgt ccagttcagg accagcaaga ccgtcgccgc gaccagcccg
      661 ccgccgaggg tgttgtcgtt gttgatcatg gcgttttgga ccgcgttgcc gagaatgatc
      721 accatgacca ggtcgtagag gctcagctgc ccgagctcgc gcttgccgag cagccggagg
      781 ccgaggatca agaaggcata gatgatggcc gtcttggccg cgatcgtcgc cgccccgttg
      841 acgctcggat agtcgaagac ctgcgcgagc aggggcatgc gaggactcgc cctcccgtgg
      901 ctggccggtt ccggcaatat cttggtccgt gccgacggtc gccgctgaca gcagctcctc
      961 aatgggcacc ctgctcatcg tcgcagggtc gaccgcggcg atctacctgt tcctgatcac
     1021 gatcctgcgt ttcgtcggac gtcgacagct ggcccagctg agcgcactcg acctcgtggt
     1081 gttcttgctg ctgggcagcg cggtggagac ggcgatgatc cacggggacg tgtcactcgc
     1141 cgccgggctc gtggcagccg cgacgttgct catcctgaac cggctgttga cgctggtctt
     1201 tctgcacgcc ccgcagctga gccatctcgt gaacggcgga ccggtcctgc tggtccacga
     1261 cggcgcggtc atcgaggagc atctccgccg ggtcggcctc acccatcccg atctcgacgc
     1321 cgcgctgcgg tcgcgggggt ttgcgagctc ggacggggtt cgcgaggcgg tgctcgagac
     1381 cgacgggagc atcaccgtgg tcggggaacg gggtgggcca tgacgactcg gcgaggcgcg
     1441 accccgcccg gtgaatccgg gcggcgttga aggtcacgcg ccaaagcatc aaccgccggg
     1501 tgagcgacag tgcggggtgg cgcacgagga gcctgccggt gagactcgag gtgtcgtgac
     1561 caggctgaca cgctcgtgac ccccaccgtc gtgctcgtgg tgagcgcctt cttggcgtcg
     1621 gccgtcgaga tggtcgaggc gctcacgatc gtgttggcgc tcggcgtcac ccgtgggtgg
     1681 cggtcggtga cgatcggggc cgcggcggcg gtcgcggcac tgaccctggt cgtcgcgacc
     1741 ttgggtcccg cgctgacgca ggttccgatc tcggccttgc ggctggtcgt cggcaccgtg
     1801 ctcctcgtct tcggcttgca gtggctccgc aaggcgatct tgcggtccgc cggggtgcgg
     1861 gcgctccacg acgaggacga ggtgttcgcc cgcgagatcg acgaatcgcg tcacgccgac
     1921 cgtcgcaccg tcgcgcgact cgattggtac gcgttcaccc tctcgttcaa aggcgtgttc
     1981 ctcgagggcc tcgaggtggc gttcatcgtc gtgaccttcg gcagcaccga aggccacatc
     2041 gggttggccg ccgccggcgc cggcgcggca ctgatcgtcg tcggcacgat cggcgccttc
     2101 gtccatcggc ccctcagccg cgtgccggag aactcgttga agttcgcggt cggcgtcatg
     2161 ttgagcgcct ttgggatgtt ctggtccgcc gagggcgccg gggtgtcgtg gccgggcg
//
LOCUS       DATCCH010000363         4763 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_19915, whole genome shotgun sequence.
ACCESSION   DATCCH010000363 DATCCH010000000
VERSION     DATCCH010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4763
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19915"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="VIJ44_06935"
     CDS             <1..796
                     /locus_tag="VIJ44_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HEY5165666.1"
                     /translation="PPERLERLTPVAHHIAELVNMALNPQAPYVGRSAFAHKAGLHVS
                     AIAKRPDAYEHIPPDQVGNGTRFVVSELAGRSTILLKAKELGLTLDGPQVNDVVDALK
                     HLEHEGYHFEVADGSLELLMRRATGWEQPWFDVESFRVMTDDRPADDLEGVSTEATIK
                     VHVNGERIIRAAEGNGPVNALDTALRAALGTRFPCLDRVHLTDYKVRVLDTQKGTGAV
                     TRVLIDTTNGDRSWTTIGVSENIIEASWQALYDSLVYGLLLDTTTD"
     gene            827..1375
                     /locus_tag="VIJ44_06940"
     CDS             827..1375
                     /locus_tag="VIJ44_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165667.1"
                     /translation="MAADPFVAPTLDDVPRQEPNLAPGVHMPAARPWLPDRPGDVVAG
                     GQPHGPLLGDPGPNIGYARTLAERLHDRLQLAPTEHLEDAVTVVSELAMKRASSYGRA
                     PVMADLECAALILGYLGGCDPEDAVWRAAAVTEASHDYPTRRAICDAVDLDALRLAPQ
                     VLSSRTRELRAQVRAAWLPGDA"
     gene            1454..2614
                     /locus_tag="VIJ44_06945"
     CDS             1454..2614
                     /locus_tag="VIJ44_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014742683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY5165668.1"
                     /translation="MGTFVLSDEHEALRASVRRFAEEQVAPYAAEADERAEFPWKAFE
                     AYRHSGFIGLPYPEEHGGDGADRVAYALLVEEIARVDASSSLFVIISRLASAPILAHG
                     SPGLQHHVIPKVAAGDWQGSYCLSEPHAGSDVASISTRAQRDGDHYVLSGRKFWITNA
                     GVSDFYTVFAKTDPDRGRGGISAFVVEGDTDGLSVGKLESKLGVRGSPTGEILLDDVA
                     VPAEHLVGEENAAFGYAMEALNGSRPIIGAQAVGIAQGALDIAAQYAMERSQFGSHIA
                     DFQGVQFMLADMATRLEAARLLVYRACSLLDSGSPDVASAAAMAKLFASDAAMQITTD
                     AVQVLGGAGYTRDFPVERMMRDAKITQIYEGTNQIQRIVVARGLLERMTERS"
     gene            complement(2723..3385)
                     /locus_tag="VIJ44_06950"
     CDS             complement(2723..3385)
                     /locus_tag="VIJ44_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165669.1"
                     /translation="MADTKWVRIAALAGLVFVVLIIVSGPVLHNSTPNITDSAQKIFD
                     SFKNHQRNIKASAALSGLAMSAVLIWASGLFHALRRAGGGNAGLAVTALGGVALAAAM
                     TVTSAAIGAATALRIHDLGPVGARFYFTLQQFTQGGTLFGLLVVVGATAAVCLRTGLF
                     ARWFAVVSVILAVGSIAGAFSIAYASNSIQTAGGIMLSLDTLWILVVSVYLWRKPELA
                     LQ"
     gene            3653..4078
                     /locus_tag="VIJ44_06955"
     CDS             3653..4078
                     /locus_tag="VIJ44_06955"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY5165670.1"
                     /translation="MFRKNKVERQLAQVPLFNRLSIDEVGRISHLLARIHRPAGETLT
                     REGEPGHEFMLVLEGSVQVLREGHSDVTLGPGSYLGEIALLESGAVRTATAVTTAPSL
                     IGYLSRDDFTSLLAESPELATQIERVMEERRGRDRQDFG"
     gene            4359..>4763
                     /locus_tag="VIJ44_06960"
     CDS             4359..>4763
                     /locus_tag="VIJ44_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018500334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5165671.1"
                     /translation="MDREARAGERAITVFLADDNVIVREGVRAILALEPDLEIVGTAG
                     DYDELVAGSEAAAPQVLVTDIRMPPSFQSEGIDAAKELRKRHPGTGIVVLSQYDDPEY
                     AVSLLRDGAAGYAYLLKDRVAEGDQLARAVRAV"
BASE COUNT          788 a         1733 c         1537 g          705 t
ORIGIN      
        1 cccccccgag cggctcgagc gtctgacccc ggtggcgcat cacatcgccg agctcgtcaa
       61 catggccctg aacccccagg ctccctacgt cggccgttcc gcgttcgccc acaaggccgg
      121 gttgcacgtc agcgcgatcg ccaaacgccc cgacgcctac gagcacatcc cgcccgacca
      181 ggtcggcaac ggcacccgct tcgtcgtctc cgagctcgcc ggccggtcca ccatcctcct
      241 caaagccaag gagctcggcc tcaccctcga cgggcctcag gtcaacgacg tcgtcgacgc
      301 cctcaagcac ctcgaacacg agggctacca cttcgaagtc gccgacggat ccctcgagct
      361 cctcatgcga cgcgccaccg ggtgggaaca gccgtggttc gacgtcgagt cgttccgggt
      421 catgaccgac gatcggccgg ccgacgacct cgaaggcgtc agcaccgagg cgacgatcaa
      481 ggtgcatgtc aacggggagc gcatcatccg cgccgccgaa ggcaacgggc ccgtcaacgc
      541 gctcgacacc gccttgcgcg ccgcgctcgg aacccggttc ccctgcctcg accgggtgca
      601 cctcaccgac tacaaggtgc gcgtcctcga cacccagaag ggcaccgggg ccgtcacccg
      661 agtgttgatc gacaccacca acggtgaccg gtcctggacc accatcggag tgagcgagaa
      721 catcatcgag gcgtcctggc aggccctcta cgactcgctc gtctacggtc tcctcctcga
      781 caccaccacc gactagctca ccaccccaag gcgaggacgc gaccacgtgg cagccgatcc
      841 gtttgtcgcc cccaccctcg acgacgtccc ccgccaagaa cccaacctcg ccccgggggt
      901 ccacatgccc gcggcgcggc cctggttgcc cgaccgtccc ggcgatgtcg tcgccggcgg
      961 ccaaccgcac ggcccgctcc tcggcgaccc cgggcccaac atcggctacg cgcgcaccct
     1021 cgccgaacgg ctccacgacc gcctccagct cgctcctact gagcacctcg aggacgcggt
     1081 gaccgtcgtc agcgagctgg ccatgaagcg cgcctcgtcg tacggacgag caccggtcat
     1141 ggccgacctc gagtgcgcgg ccctcatcct cggctacctc ggcggctgcg accccgagga
     1201 cgccgtctgg cgggccgccg ccgtcaccga ggcctcccac gactacccga cccgtcgagc
     1261 gatctgtgac gccgtcgacc tcgacgcgct ccgtctcgcc ccccaggtcc tgagcagccg
     1321 aacccgcgag ctccgggcgc aggtccgcgc cgcctggctt cccggtgacg cctgagcctc
     1381 accgggaacc tgtcggaggg cctccccgcg tttgctgacg tacacgtcag gtagccggat
     1441 atcctggggc ctggtgggca cgttcgtcct ctccgacgag cacgaggcgc tgcgggcctc
     1501 ggtccgtcga ttcgccgagg aacaggtcgc gccctacgcc gccgaggccg acgagcgcgc
     1561 cgagttcccc tggaaggcct tcgaggcgta ccgccactcc ggcttcatcg ggctgcccta
     1621 ccccgaggag cacggcggcg acggggccga ccgggtcgcc tacgcgctgc tggtcgaaga
     1681 gatcgcccgg gtcgacgcgt cctcctcgct cttcgtgatc atcagccgct tggccagcgc
     1741 cccgatcctc gcccatggat cccccggcct tcagcaccac gtcatcccga aagtcgccgc
     1801 cggcgactgg caaggctcct attgcctctc cgagccccac gcgggcagcg acgtcgcatc
     1861 gatctcgacc cgcgcgcagc gcgacgggga ccactacgtc ctgtcgggcc ggaagttctg
     1921 gatcaccaac gccggcgtct ccgacttcta caccgtcttc gccaagaccg acccggaccg
     1981 cgggcggggc gggatctcag cgttcgtcgt cgagggggac accgacgggc tcagtgtcgg
     2041 caagctcgaa tcgaagctcg gggttcgagg atcgcccacc ggcgagatcc tcctcgacga
     2101 cgtcgccgta cccgccgagc atctggtcgg ggaggagaac gccgcgttcg gctacgcgat
     2161 ggaggcactc aacggttccc ggcccatcat cggcgcccag gccgtcggca tcgcccaagg
     2221 cgccctcgac atcgcggcgc agtacgccat ggaacggtcc cagttcggca gccacatcgc
     2281 cgatttccaa ggtgtccagt tcatgctggc tgacatggcg acccggctcg aagccgcccg
     2341 actcctcgtc taccgcgcct gttccttgct cgattccggt tcccctgacg tcgcgtccgc
     2401 cgcggccatg gccaagctct tcgcctcgga cgccgccatg cagatcacca ccgacgccgt
     2461 ccaggtgctc ggcggcgcgg gctacacccg ggactttccc gtcgagcgca tgatgcgcga
     2521 cgccaaaatc acccagatct acgaaggcac gaaccagatc caacggatcg tcgtcgcacg
     2581 aggcctcctc gagcgcatga cggagcgttc gtagacggct gatcgagccg aggccggcgg
     2641 cgccgagcgg cgtgtacgcc gcaccaggct gtgtgctgtc gcggcccgac cgctcgccga
     2701 ccgggccacg acgccgtccg tgttactgga gcgcgagctc cggcttgcgc cagagataca
     2761 cgctcacgac gaggatccac agggtgtcca gcgacagcat gatcccgccg gccgtctgga
     2821 tggagttgct ggcgtaggcg atcgagaacg cgcccgcgat ggatcccacg gccaggatga
     2881 cgctgacgac ggcgaaccag cgggcgaaca gcccggtgcg aaggcacacc gccgcggtcg
     2941 caccgacgac cacgagcagt ccgaacagcg ttccaccttg ggtaaactgt tgaagcgtga
     3001 aatagaagcg cgcaccaacc gggcccaggt catggatacg gagcgccgtc gcggcaccga
     3061 tcgcagcgct ggtcaccgtc attgccgcgg cgagggcgac accgccgagc gcggtgaccg
     3121 ccaagcccgc gttgccgccc cccgctcgtc gcagagcatg aaacaggccc gaggcccaga
     3181 tcaggaccgc tgacatcgcc aggccggaga gtgcggcgct ggccttgatg ttgcgttgat
     3241 ggttcttgaa cgagtcgaag atcttctgcg ccgagtccgt gatgttcggc gtgctgttgt
     3301 gcaggacagg acccgaaacg atgatcagca ccacgaacac cagaccggcc agcgcagcga
     3361 ttcgtaccca cttggtgtca gccatgtccc ctctttcgtc ggtgtagaac cacgccacgc
     3421 ggcactggtt ctgtcggcca tatggccctc taactgagtg tctcgggcac cgtacgaagc
     3481 catccgagcc gtggcgaggg ggctagcccc gcaaccgcgg gggcgttggc acgaccacgc
     3541 tcgggacgtg agcactctgg ctgcgagacg gcactgggct tcaatgaagc cgaagtggcc
     3601 gacggttcgg cggccaagca atcgcggtac ggtcctgcac agggaggcgc cgatgttccg
     3661 gaagaacaag gtcgaacgac agttggcgca agtgccgctc ttcaaccggc tctcgatcga
     3721 cgaggtgggt cggatcagcc atctcctcgc tcgcattcat cgaccggcgg gcgagaccct
     3781 gacccgcgag ggtgagcctg gccacgagtt catgctcgtc ctcgagggaa gcgtgcaggt
     3841 tctgcgagag ggtcacagcg acgtcaccct cggtccgggc tcgtatctcg gcgagatcgc
     3901 gctgctggaa agcggggccg tccgtacggc gacggcggtc acgacagctc cgtcactgat
     3961 cggctacctc agccgtgatg acttcacgtc cttgttggcc gagtcgccgg agctggccac
     4021 gcaaatcgag agggtcatgg aggagcgccg cggtcgcgac cggcaggact ttggctgacg
     4081 acccttgagc gcgggggaat ttccaatgct caacccggcg gcttcgagcg acggtcggcc
     4141 tggtggcctc ggccatggcg gccccgggat atcggtggct gaggtcggcg gtgctcggcc
     4201 tggcccggag ctgaccctga cgaccgaagc gacggtccgg gttcggtgcc gcgtcggacc
     4261 ggcacggccc cttcggggcc gaggggaggc tggcatcccc gaggttgcgg gagtgagcca
     4321 ccttgccggc gccgccgcga cgggggctca atggacccat ggaccgcgag gccagggccg
     4381 gagagcgggc gatcacggtg ttcctcgcgg acgacaacgt gatcgtgcgc gagggcgtcc
     4441 gggccatcct cgccctcgag cctgacctcg agattgttgg caccgcgggc gactacgacg
     4501 agctcgtcgc cggttccgaa gcagcggcgc cccaggttct cgtgaccgac atccggatgc
     4561 ctccgagctt tcagagcgaa ggcatcgacg cggcgaagga gctccgcaag cggcacccgg
     4621 gcacgggcat tgtggtgctt tcgcagtacg acgacccgga gtacgcggtg tcattgctcc
     4681 gggacggcgc cgccggatat gcctacctcc tcaaggaccg ggtcgccgaa ggtgaccagc
     4741 tcgcccgagc ggtgcgcgcg gtc
//
LOCUS       DATCCH010000364         2250 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_61590, whole genome shotgun sequence.
ACCESSION   DATCCH010000364 DATCCH010000000
VERSION     DATCCH010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61590"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1044)
                     /locus_tag="VIJ44_06965"
     CDS             complement(<1..1044)
                     /locus_tag="VIJ44_06965"
                     /inference="COORDINATES: protein motif:HMM:NF013067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HEY5165672.1"
                     /translation="MTEPGAAVVLCVNCGSSSVKCAVYELGSGHEDRLAASSVDGSGP
                     AVLDEALRVADPFEAALTGAGHRIVHGGPHHYRPSRIDDALIADLQAAMPFAPLHLPA
                     ALTGIEAVTRRHPGLPQVACFYTAFHRTMPETSWHLPLPPALVEAGVRRYGFHGLSYE
                     YIVAHVGAETLGRAVLAHLGSGASLAAVRDGRSVHTTMGFTPSGGIVMSSRSGDLDPG
                     VLIYLTRQLGYDATRLEQLIDTESGLRALSGETSDMRALLDRRDAGDQRADLAVAVFC
                     SHIRMQIGAYAALLGGLDTVVFTGGIGAHATAVRAESCGGLAHLGLVIDEHRNASAEP
                     VISDPDSRVTVWAL"
     gene            complement(1041..>2250)
                     /locus_tag="VIJ44_06970"
     CDS             complement(1041..>2250)
                     /locus_tag="VIJ44_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019749186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoketolase"
                     /protein_id="HEY5165673.1"
                     /translation="GAELHESTRRLGELLRDIFVNNRDAANFRLVCPDETNSNRLGAV
                     FDVTDRCSLEPTIAIDDHVSADGRVMEILSEHNCQGWLEGYVLTGRHGLFASYEAFAM
                     VSASMLIQHAKWLEAAADLPWRVPVSSLNVLLTSTCWRNDHNGFSHQGPGLIDTVITK
                     RGNVARIYLPPDANCLLAVADHCLRSRDCVNLIVIDKQPQPQWLDLETAREHCRYGAS
                     VWAWAGSTAAGDEPDVVLASAGDIPTLELLAAADLLHQYVPELALHVVNVVDLMALFT
                     PDQHPHGMDAGRFVDLFTDERDVVFAFHGYPGAIHHALHGRPNASRFHVRGYNEEGTT
                     TTPFDMVVRNGISRYHLCLEALKRARRFPSDAESLIAYCNAQLKRHPGYIAQHLEDMP
                     EVRDWRWSGP"
BASE COUNT          372 a          722 c          824 g          332 t
ORIGIN      
        1 gagggcccag acggtgacgc gagagtcggg atcgctgatc accggttccg ccgacgcgtt
       61 ccggtgctcg tcgatcacga gcccaaggtg ggccagaccc ccacacgact cggcccgcac
      121 cgccgtggcg tgcgcaccga ttccgccggt gaacaccacg gtgtcgaggc cgccgagcag
      181 cgcggcgtag gcgccgatct gcatccggat gtgcgagcag aacaccgcaa ccgcgagatc
      241 ggcccgttgg tccccggcgt ctcgtcgatc gagcagcgcc cgcatgtccg aggtctcacc
      301 ggagagcgcc cggagacccg attcggtgtc gatcagctgc tcgaggcggg tggcgtcgta
      361 gccgagctgg cgggtcaggt agatgagcac gccggggtcg aggtcgccac tgcggctact
      421 catgacgatc cctcccgacg gggtgaatcc catcgtggtg tgcaccgagc gtccgtcgcg
      481 caccgcggcg aggctggcac cactgccgag gtgggcgagg acggcacgcc cgagggtctc
      541 ggcaccgacg tgggcgacga tgtactcgta cgacaggccg tggaacccgt agcggcgcac
      601 cccggcctcg acgagggccg gcgggagggg aaggtgccac gaggtctcgg gcatcgtgcg
      661 gtggaacgcg gtgtagaagc aggcgacctg gggcaagccg gggtggcggc gcgtgaccgc
      721 ctcgataccc gtgagtgcgg cggggagatg cagcggcgcg aacggcatgg ccgcctgcag
      781 gtccgcgatc aacgcgtcat cgatccggga cggccggtag tgatgggggc cgccgtggac
      841 gatccggtgg ccggcgccgg tcagcgccgc ctcgaacgga tccgcgaccc gcagcgcctc
      901 gtcgaggacc gcgggcccgg agccgtcaac gctcgacgcc gcgagccgat cttcgtggcc
      961 agatccgagc tcgtagacgg cgcacttcac cgacgacgac ccgcagttga cgcacaggac
     1021 gaccgccgcg cccggctcgg tcacggaccc gaccagcgcc aatcacggac ctcgggcatg
     1081 tcttcgagat gctgcgcgat gtatcccggg tgccgcttca gctgcgcgtt gcaatacgcg
     1141 atgaggctct cagcgtcgga cgggaagcgg cgggctcgtt tgagggcttc caggcacagg
     1201 tggtagcggc tgatgccgtt gcggaccacc atgtcgaacg gcgtcgtggt ggtgccctcc
     1261 tcgttgtagc cgcggacatg gaaccgtgac gcgttcggcc gaccatgcag cgcatggtgg
     1321 atcgcaccgg ggtagccgtg gaaggcgaac acgacgtcgc gctcgtccgt gaagaggtcg
     1381 acgaaccggc cggcgtccat gccgtgcgga tgctggtcgg gcgtgaacaa ggccatgagg
     1441 tcgacgacgt tgacgacgtg gagcgcgagc tcgggaacgt attggtgcag gagatcggcg
     1501 gcagcgagca gctccagggt ggggatgtca ccggctgacg ccaagacgac gtcaggctcg
     1561 tcgccggccg cggtcgaccc ggcccaggcc cacaccgacg cgccataccg gcagtgttcg
     1621 cgcgccgtct cgagatccag ccactgcggc tggggctgct tgtcgatgac gatgaggttg
     1681 acgcagtcgc ggctccgcag gcagtggtcg gcgacggcga ggagacagtt ggcgtccgga
     1741 ggcaggtaga tccgagcgac gttgcctcgc ttcgtgatga ccgtgtcgat cagcccgggg
     1801 ccttggtggc tgaacccgtt gtggtcgttg cgccaacacg tcgaggtcag cagcacgttc
     1861 agcgacgaca ccgggacccg ccacggaagg tcggcggcgg cttcgagcca tttcgcgtgt
     1921 tggatcagca tggacgccga gaccatcgcg aacgcttcgt agctcgcgaa cagcccgtgc
     1981 cgtccagtca ggacgtagcc ctcgagccac ccctgacagt tgtgctcact caagatctcc
     2041 atcacgcgcc cgtccgcgct gacatggtca tcgatcgcga tggtcggctc gagcgagcac
     2101 cggtcggtca cgtcgaagac ggcgccgagc cggttggagt tggtctcgtc ggggcagacg
     2161 agacggaaat tggcggcgtc acggttgttg acgaagatgt cccggagcag ctcgccgagg
     2221 cgacgagtcg actcatggag ctcggcaccg
//
LOCUS       DATCCH010000365         2281 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_394333, whole genome shotgun sequence.
ACCESSION   DATCCH010000365 DATCCH010000000
VERSION     DATCCH010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394333"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="VIJ44_06975"
     CDS             complement(<1..412)
                     /locus_tag="VIJ44_06975"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEY5165674.1"
                     /translation="MPSGDADPGTALRQRGPLVVGIVAIVVALDQATKAWAVDSLARA
                     PVSIIGTTVEFRLSRNMGSAFSLFQTLTPLLAVLAVAIALILVRAMRRTDDRVVIIAL
                     ALVLAGAFGNLGDRLFRSPGWLRGAVVDFIHLGGW"
     gene            complement(393..896)
                     /locus_tag="VIJ44_06980"
     CDS             complement(393..896)
                     /locus_tag="VIJ44_06980"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HEY5165675.1"
                     /translation="MAGKTKPTFAPLPPPAGTPTKSRGGKKIVLCPLSGFEVTPAPAN
                     LSAKTLDRLRQKLLDEKQRLRHQAEELTAEAEQLAREREAGDTQFDEESGEGDTVNIE
                     RERDLLLSATARQVVEEIDDAIERIKRGKYGSCKPAGRKISLERLEAIPWAQVCVDCK
                     ARAERRR"
     gene            complement(1417..1809)
                     /locus_tag="VIJ44_06985"
     CDS             complement(1417..1809)
                     /locus_tag="VIJ44_06985"
                     /inference="COORDINATES: protein motif:HMM:NF014633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein"
                     /protein_id="HEY5165676.1"
                     /translation="MRGSQADHRNGIAISDVDDLYSIEDDTIVLSVHVQPRAGRSAVL
                     GRHGRALKLRVAAPPVDERANAAATTLLAEEFGVRSGDIELIGGQRSRLKRFRIRNVD
                     QDEFATRLRRTVESADRGGGPRSRHRAE"
     gene            1826..>2281
                     /locus_tag="VIJ44_06990"
     CDS             1826..>2281
                     /locus_tag="VIJ44_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014135256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEY5165677.1"
                     /translation="MPFESLGRELDLVALEDRVLRRWVDNDVFAESLRRREGAPEWVF
                     YEGPPTANGRPGLHHVWARSFKDLYPRFHTMRGRYVARKGGWDCHGLPVELEVERELG
                     FSGKPDIEDYGIARFNQRCRDSVHRYVEDWSALTTRIGMWIDTANAYWTL"
BASE COUNT          289 a          719 c          844 g          429 t
ORIGIN      
        1 gccacccgcc gaggtggatg aagtcgacga ccgcgccgcg caaccagccc ggggagcgga
       61 agagccggtc gccgaggttg ccgaacgccc cggcgaggac cagggccagc gcgatgatca
      121 cgacccggtc gtcggtccgt cgcatggctc gcacgaggat gagcgcgatc gcgaccgcga
      181 ggactgcgag gagcggggtc agggtctgga agagactgaa ggcgctcccc atgttgcgac
      241 tgaggcggaa ctcgacggtg gtgccgatga tggagaccgg cgcgcgcgcg agcgagtcga
      301 cggcccaggc tttcgtggcc tggtcgaggg caacgacgat ggcgacgatc ccgacgacca
      361 aggggccgcg ctgacggagc gcggtcccgg ggtcagcgtc gccgctcggc acgcgccttg
      421 caatcgacgc acacctgcgc ccacgggatc gcctcgagac gttcgaggga gatctttcgg
      481 ccggcgggct tgcaggagcc gtacttcccg cgtttgatcc gctcgatcgc gtcgtcgatc
      541 tcctcgacga cttggcgggc tgtggcggag agcaacaggt cgcgttcgcg ctcaatgttc
      601 acggtgtcgc cttcaccgga ttcttcgtcg aactgggtgt cgccggcttc gcgttcgcgt
      661 gccagctgtt cggcttcggc ggtgagctcc tcggcttggt ggcggaggcg ttgcttctcg
      721 tcgaggagct tctggcggag ccggtcgagg gtcttggcgc tgaggttggc tggggccggg
      781 gtcacctcga atcccgacaa gggacagagg acgatcttct tgccgccgcg gcttttggtt
      841 ggtgtgccag ccgggggcgg gagcggggcg aaggtgggtt tggtcttccc ggccactgcg
      901 gccgtggact tggctggcgc ggccttcttg cccgcgacgc ttgtggtggg cgatgaccgc
      961 ttcgccgtgg cgcccccggg ggcgggcggc gccttcttgg cggtgggcct cgtcgaggcg
     1021 gccttcttcg agcccgcctt cttcgagctg gccttctttg gggccgcctt cttcgagctg
     1081 gccttcttcg gggcagactt ctttgggggc gacttcttcg agcccgactt cttcggggca
     1141 gacttctttg gggcggcctt ctttgccgtc gacttcgccg gtggcttccg ggcggccttc
     1201 gtggccgcgg gcttcttggc accggtcttc ttcggcgcca tccgcttcgc tccactggac
     1261 cggttgcggg acgtcgcgcc ccggcgagcc gccggcttct tgcctgccgt gcccggcatc
     1321 tcggtctccc ttggcccgcg ggggccggat cggaccccga aagagcggtg gatggtagcg
     1381 cccccgatca ggctcctcca gcgggacgtt cgggggtcac tcggcccggt gacgcgaacg
     1441 cggcccgccc ccgcggtcgg cggactccac ggttcggcgg agccgcgtcg cgaactcgtc
     1501 ttggtcgacg ttgcggatcc ggaaccgctt gagccgggac cgctgacctc cgatgagttc
     1561 gatgtcaccg gacctcacac cgaactcctc ggcgagcagc gtggtcgcgg cggcattggc
     1621 gcgctcgtcg accggaggcg cggccacgcg gagcttgagg gcgcgtccgt ggcggccgag
     1681 gacggcgctg cgcccagcgc gcggctggac atgcaccgag aggacgatgg tgtcgtcttc
     1741 gatcgagtac aggtcgtcga cgtcgctgat ggcgatcccg tttctgtgat cggcttgtga
     1801 acccctcacg atacgctcgg cggcgatgcc cttcgagtcg ttaggtcgcg agcttgatct
     1861 cgtcgcgctc gaggaccgtg tgctgcgacg ttgggtagac aacgacgtgt tcgccgaaag
     1921 tcttcgtcgc cgtgagggcg cgcccgagtg ggtgttctac gagggaccgc cgaccgcgaa
     1981 tgggcggcct ggtctccatc atgtctgggc ccggtcgttc aaggatctgt acccgcgttt
     2041 tcacaccatg cggggccggt atgtggcccg gaagggtggc tgggactgtc acgggctgcc
     2101 ggtggagctc gaggtggagc gtgagctggg gttctccggc aagcccgaca tcgaggacta
     2161 cgggatcgcg cggttcaacc agcgctgtcg tgactcggtg catcgatacg tcgaggattg
     2221 gtcggcgctc acgacgcgta tcgggatgtg gatcgacacc gcgaacgcct actggacgtt
     2281 g
//
LOCUS       DATCCH010000366         3224 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_290295, whole genome shotgun sequence.
ACCESSION   DATCCH010000366 DATCCH010000000
VERSION     DATCCH010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290295"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..139)
                     /locus_tag="VIJ44_06995"
     CDS             complement(<1..139)
                     /locus_tag="VIJ44_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007075302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEY5165678.1"
                     /translation="MTRVGVFGAAGRMGRTVCAAIAAEPDLDLVAAVDPYHAGIDLQQ
                     LG"
     gene            complement(149..1405)
                     /locus_tag="VIJ44_07000"
     CDS             complement(149..1405)
                     /locus_tag="VIJ44_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY5165679.1"
                     /translation="MIRRTRLDSGLRVVTEAMPQLRSVAIGFWVGTGSRDEPADWAGV
                     SHFLEHLLFKGTERRDAATIAEAIESVGGDMNAFTAQEVTTYYVRVPDERLDLAADIL
                     SDIVWHPSLRPDEIESERKVILEELHMRDDEPEDLVHDVFTSAVFPEHPIGREVIGSA
                     ETIQAMQRDDIAAFHHRHYHPSNVVVAAAGNLDHDHIVDLVAQAVDGLNGNRPPRDRY
                     LGEPPPRALALLERPAEQAHLVLGMRALPHQDPDRYALTVLNQALGGGMSSRLFQEVR
                     EQRGLAYSVYSYRAALEDTGILAVSAGTAPERFDELLAVLEDQLDRVVADGGVSDRER
                     RSAQGHLRGSLALSLESSGSRMHRLGRSELTLGEIPNLDELVARIDAVSTADITRVVE
                     RVLIPGRRTLAVVGPVSPERFADRVA"
     gene            complement(1451..>3224)
                     /locus_tag="VIJ44_07005"
     CDS             complement(1451..>3224)
                     /locus_tag="VIJ44_07005"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEY5165680.1"
                     /translation="ELVRKVQEANGPIQEVAYEPRFDYDDDVFDAVADSVRAGTIEAM
                     AIADKTARNSRLDEIESAIIAELAGADEAPFADRAGQVKKAFRSLQKQVVRTRIVEEG
                     IRIDGRSPGDLRALSADISIIPTAHGTGLFQRGETQVLSVATLGMPRMEQMLDTIGID
                     DRKRYMHHYNFPPFSTGETGRVGSPKRREIGHGALAERALVPVLPSAEEWPYTMRVVS
                     DVLSSNGSTSMASVCGSTLSLMDAGVPIKQPVAGIAMGLVHADGKYTTLTDILGAEDA
                     FGDMDFKVAGTREFVTALQLDTKIDGLPADVLTQALRQAHEARITILDVMNDAIANPR
                     DDVKPTAPKIISFEIPIDKIGEVIGPKGKVINTITQETGADVSVSDNGAVGTVTVGSK
                     ESSAVEEARRRIELILDPPTADLGAEYTGRVVNITKFGAFVNVLPGKDGLLHISKLGR
                     GKRIDRVEDVLSLGEEVTVRVDDIDNAGKLSLSLAGDDADGSDGQDRAPRADAPSPRP
                     ERDREPRERHDRAPREEREPRSDRGGDRGSDRGSDRGGDRDVASFEERWEAQAKDEFG
                     DLGPGEAARAGAPGGDGPRRRRRR"
BASE COUNT          438 a         1017 c         1197 g          572 t
ORIGIN      
        1 caccgagctg ttggaggtcg atgccagcgt ggtagggatc gaccgcggcg acgaggtcga
       61 ggtcgggttc cgcggcgatc gccgcgcaga ccgtgcgccc catccgaccc gcggcgccga
      121 acactccgac tcgtgtcacg ccggaaggct aggcgacccg atcggcgaag cgttcgggcg
      181 agaccggccc gacgacggcg agggtccggc ggcccgggat gaggacgcgc tcgacgaccc
      241 gggtgatatc cgcggtggag acggcatcga tgcgggcgac gagctcgtcg aggttgggga
      301 tctcgccgag cgtgagctcg cttcgcccaa gccggtgcat gcggctcccc gagctctcca
      361 acgacaaggc gagcgatccc cgcaggtgac cttgggcgct tcgacgctcc cggtccgaga
      421 cgccgccgtc ggcgacgacc cggtcgagct ggtcctcgag gaccgcgagg agctcgtcga
      481 atcgttcggg tgcggtcccg gccgacaccg cgaggatgcc ggtgtcttcg agcgcggccc
      541 ggtaggagta cacggagtac gccaagccgc gctgctcacg cacctcttgg aagaggcgcg
      601 atgacatgcc gcctccgagc gcctggttga gcaccgtcag cgcgtaccgg tcggggtcct
      661 ggtggggcag ggctcgcatg ccgaggacga ggtgggcctg ttcggccggg cgttcgagca
      721 gcgcgagggc ccgtggtggc ggctcgccca agtatcggtc gcgcggggga cggttgccgt
      781 tgagaccgtc gaccgcttgg gcgacgaggt cgacgatgtg gtcgtgatcg aggttgccgg
      841 ccgcggcgac gaccacgttg ctcgggtggt agtgacggtg gtggaaggcg gcgatgtcgt
      901 cgcgttgcat ggcttggatg gtctcggcgg agccgatcac ttcgcgtccg atgggatgtt
      961 cggggaacac ggcgctggtg aatacgtcat ggacgaggtc ttcgggttcg tcgtcacgca
     1021 tgtggagctc ttcgaggatg accttgcgtt ccgattcgat ctcgtcgggg cgcagcgacg
     1081 ggtgccacac gatgtcggag aggatgtcgg ccgccaggtc gaggcgctcg tcggggacgc
     1141 ggacgtagta ggtggtgact tcttgggcgg tgaacgcgtt catgtcgccg ccgaccgatt
     1201 cgatggcttc ggcgatcgtg gccgcgtcgc gccgttcggt ccctttgaag aggagatgct
     1261 cgaggaagtg gctgaccccg gcccagtcgg ccggttcgtc gcgggagccc gtcccgaccc
     1321 agaagccgat cgcgaccgac cggagctgtg gcatcgcttc ggtgaccacg cgcaggcccg
     1381 agtccagacg ggtgcgcctg atcatctggc cgtgatcggc atcgccgcgc aggggttgag
     1441 cctcggccgg ttagcggcga cggcgacggc gggggccgtc accaccggga gcgccggcgc
     1501 gggccgcttc gcccggcccg aggtcaccga actcgtcttt ggcttgggcc tcccagcgct
     1561 cctcgaacga cgccacgtcc cggtcgccac cgcggtcgct gcctcggtcg ctgcctcggt
     1621 cgccgcctcg gtcgctgcgt ggttcgcgct cctctcgtgg ggcgcggtca tgacgttcgc
     1681 ggggttcacg gtcgcgctcg ggtcgtgggg acggagcatc ggcccgcggg gcgcggtcct
     1741 ggccgtcgga cccgtcggcg tcgtcgccgg cgaggcttag cgagagcttc ccggcgttgt
     1801 cgatgtcatc aactcggacg gtgacttctt cgccgaggct gagcacgtct tcgacccggt
     1861 cgatgcgctt gccgcgaccg agcttggaga tgtgcagcag cccgtccttg ccggggagca
     1921 cgttcacgaa cgcgccgaac ttggtgatgt tgacgaccct gccggtgtac tcagcgccga
     1981 ggtccgcggt gggcgggtcg aggatcagct cgatgcgtcg acgggcttct tccaccgccg
     2041 acgattcctt ggatccgacc gtgacggtcc cgaccgcccc gttgtcggag accgacacgt
     2101 cggcgccggt ctcttgggtg atggtgttga tgaccttgcc cttcgggccg atcacctcgc
     2161 cgatcttgtc gatcgggatc tcgaagctga tgatcttcgg cgcggttggc ttcacgtcgt
     2221 cgcgggggtt ggcgatcgcg tcgttcatca cgtcgaggat ggtgatgcgt gcttcgtggg
     2281 cttggcggag cgcctgggtg aggacgtccg cggggagtcc gtcgatcttg gtgtcgagct
     2341 gaagggcagt gacgaactcc cgggtgccgg cgaccttgaa gtccatgtcg ccgaacgcgt
     2401 cttcggcgcc gaggatgtcg gtgagggtcg tgtacttgcc gtcagcgtgg acgaggccca
     2461 tggcgatgcc ggccacgggc tgcttgatgg gcacgccggc gtccatgagc gacagggtcg
     2521 atccgcacac cgatgccatc gacgtggagc cgttcgacga aaggacgtcg gagacgacgc
     2581 gcatcgtgta cggccattcc tctgcgctcg gcaggaccgg gacaagagcc cgctccgcga
     2641 gcgcgccgtg gccaatctcg cgacgcttcg gtgatccgac ccggccggtc tccccggtcg
     2701 agaacggcgg gaagttgtag tggtgcatgt agcgcttgcg gtcgtcgatg ccgatggtgt
     2761 cgagcatctg ctccatgcgc ggcataccga gcgtggcgac gctgagcacc tgggtctcac
     2821 cccgctggaa gaggccggtg ccgtgcgcgg tcgggatgat gctgatatcg gccgacaggg
     2881 ctcgaaggtc gcccggggag cggccgtcga ttcggatgcc ctcctcgacg attcgggtcc
     2941 gcacgacttg cttctgcagc gagcggaacg ccttcttgac ctgcccggcc cggtcagcga
     3001 acggggcctc gtcagcgccg gccagctcgg cgatgatcgc cgattcgatc tcgtcgaggc
     3061 gggagttgcg cgccgtcttg tcggcgatcg ccatcgcttc gatggtcccg gcccgcaccg
     3121 agtcggccac ggcgtcgaag acgtcgtcgt cgtagtcgaa gcgcggctcg taggcgacct
     3181 cttggatcgg accgttggcc tcttggacct tgcggaccaa ctcg
//
LOCUS       DATCCH010000367         5605 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_435780, whole genome shotgun sequence.
ACCESSION   DATCCH010000367 DATCCH010000000
VERSION     DATCCH010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5605
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435780"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..1593)
                     /locus_tag="VIJ44_07010"
     CDS             complement(130..1593)
                     /locus_tag="VIJ44_07010"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEY5165681.1"
                     /translation="MQLQELLADVDVDVRGDARVEVITLEHDSRRVEPGACFACIVGH
                     ATDGHRHAPSAVDAGAVALLVERPLDLGVPEARVANVRRALGPAAARLYGIPSAAVRC
                     LGVTGTNGKTTTTHLLEAIATAAGERVGLIGTVGARIAGRPLPVEHTTPEATDLQRLL
                     AHMRDDGVRTVAMEVSSHALAQHRIDGTHFAAVCFTNLSRDHLDYHRTLDDYFETKAL
                     LFDPARADAAAVNLDDSRGADLVARCRARDLPVTTFGVNHAADVVATDISVTRDGSHV
                     TLVERGERTPLQIRLLGSVNVANAVAAAVTARVAGFDPSAIATGLASVVNVPGRLERI
                     ESGQPFTVLVDYAHTPAALDAALHSARDLPGSQRVIVVFGCGGERDPEKRPLMGEVAA
                     GEAEVVIVTSDNPRSEAPDAIATAILDGTRAGSAEVHVELDRRQAIRRALADAEPGDV
                     VLIAGKGHEAQQVGGGAAVDFDDRVVAREELGAGAWN"
     gene            complement(1628..3292)
                     /locus_tag="VIJ44_07015"
     CDS             complement(1628..3292)
                     /locus_tag="VIJ44_07015"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEY5165682.1"
                     /translation="MTVLVFALIGIRLVDVQARNRGRYVRLGLSQRVRTVTIPAQRGS
                     IFDRNGNALAVSVDATSISANPRVIRDPAAYAAKLAPIVGVDPATLQARLSDHRHGFV
                     YIARKVDPPVERAVRALHLIGLDYTPEPKRYYPADPLAAPVLGVVGTDNAVLSGLEAG
                     SQTALAGQNGRLVVEQDPRGRDLPNGTRQDRPELKGSDLFLTIDQSMQYQAERVLADE
                     VAGAHAKGGMAILTDVRTGNILAMVSVDGPNGAIPAHPSSGLSPNRPLTDVFEPGSTA
                     KVVTIAAAIEAGLVTPSTVLSVPQSITVDNTKYEDVDTHPTLMTVADIVRQSSNVGTI
                     EIARMLGRDRFDAAIHAFGFGAPSGTSYPGEAAGILLPRAQYNATSLASMPIGSGIAV
                     TAMQLLDVYMTIANNGVAHPPRLVTSTLDAAGHRHQVTSVADHAVVSPNTAAQVRQML
                     EGVVAGGTGTQAQISGYRVAGKTGTARKPPYLKPPYNYVASFAGFAPADAPRLAAVVV
                     LDEPQGAVYGGEVAAPAFAKMMQYALTVERVPASSPSSSAATGPIP"
     gene            complement(3483..3920)
                     /locus_tag="VIJ44_07020"
     CDS             complement(3483..3920)
                     /locus_tag="VIJ44_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165683.1"
                     /translation="MAVAAPPARVRTASRRSAAAPAPRRVAPPRRDHLRVVAPARRAR
                     RVHRSVLLAAVGTVLAVLALVTFQVMTAQSQLTLDHVSHQVDDAQRRYDQARLVNAQL
                     SSPSRIAARALLLGLVPPAAPPVPVPVPSNAVAPVSARQASQP"
     gene            complement(3926..4879)
                     /gene="rsmH"
                     /locus_tag="VIJ44_07025"
     CDS             complement(3926..4879)
                     /gene="rsmH"
                     /locus_tag="VIJ44_07025"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEY5165684.1"
                     /translation="MGGFQHQPVMVREVIELLAPVPTGTVVDATVGGGGHARRLLEAR
                     PDLRLLGIDRDPAAVAAARAELEPFGNRARIVHGGFEDVAEIVGQASEGNVMGILFDL
                     GVSSPQLDLPERGFTYWGDAPLDMRMDSAQALTAERVVNEYTETGLAEVIANNGEERY
                     ARSIASRIVAHRPLHTTGDLVTAVKEAIPARARRRGGHPARRTFQAIRMEVNRELPNL
                     AAGLDESVHIIGPQGRVLVLAYHSLEDRAVKERFTRWAGEDPASQPPPGLPVEPTRPN
                     ALARLLTRRALRPQADEVAANPRAESARLRAIEKLPSPTGP"
     gene            complement(5060..5494)
                     /locus_tag="VIJ44_07030"
     CDS             complement(5060..5494)
                     /locus_tag="VIJ44_07030"
                     /inference="COORDINATES: protein motif:HMM:NF014439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEY5165685.1"
                     /translation="MATAFIGRYQHTLDDKGRLILPADFRKALEEGAYIGQFPGGCLA
                     IYTPERFSEVSEDISQSAKEGGTEAAVHASRVFFASAQAVTPDKQGRVPVAAYLRDWA
                     HLDREVIVTGQDKRIELWSPQSWQQVEREGQAELARRQSWGI"
BASE COUNT          762 a         1840 c         2160 g          843 t
ORIGIN      
        1 ccgcgacgag ggccgcgaag cacgcgcccg ggtcgaggag ccggctgtcg atggtgaacg
       61 accgggcgcg cgcgtccggc gtcccggcca ccactcggcc accggtggcg gcggcaatct
      121 cggacgcggt cagttccacg ccccggctcc cagctcttcg cgggcgacga cccggtcgtc
      181 gaaatcgacc gcggctcccc caccgacctg ttgggcctca tggcccttcc cagcgatgag
      241 gaccacgtcg ccgggctcgg cgtcggcgag ggcacggcga atcgcctggc gccggtcgag
      301 ctcgacgtgg acctcggcgg acccggcgcg cgtcccgtcg aggatcgcgg tggcgatcgc
      361 gtcgggcgct tccgagcgcg ggttgtccga cgtgacgatc acaacctcgg cctcgccggc
      421 ggcgacctca cccatgagcg gtcgcttctc ggggtcacgc tcgccgccac agccgaagac
      481 gacgatgacc cgttgcgacc cgggaagatc ccgggcgctg tgcaacgcgg cgtcgagcgc
      541 cgcgggcgtg tgcgcgtagt cgacgagaac ggtgaacggc tggccgctct cgatccgctc
      601 caggcgcccc ggtacattca cgaccgaggc caacccggtc gcgatcgcgg aaggatcgaa
      661 cccagcgacc cgcgccgtga ccgccgccgc gaccgcgttc gcgacgttga cggagccgag
      721 cagccggatc tgcaacgggg ttcgctcccc gcgttcgacg agggtgacgt ggcttccgtc
      781 ccgagtgacg ctgatgtcgg tggcgaccac gtcggcagcg tggttcacac cgaacgtggt
      841 gaccggaagg tcgcgcgccc ggcagcgggc cacgaggtcg gcgccgcggg aatcgtcgag
      901 gttgacggcg gcggcgtcgg cccgggccgg atcgaacaag agggccttgg tctcgaagta
      961 gtcgtcgagg gtgcggtgat agtcgagatg gtcgcggctc aggttcgtga agcagaccgc
     1021 agcgaagtgg gtgccgtcga tgcgatgctg ggccagcgcg tgcgacgaga cctccatcgc
     1081 gacggtgcgg acgccgtcgt cgcgcatgtg cgcgaggagc cgctgcaggt cggtggcctc
     1141 gggggtggtg tgctccacgg ggaggggccg gccggcgatc cgggcgccca ccgtcccgat
     1201 gaggccgacg cgctctccgg cggcggtggc gatggcctcg agcagatggg tggtggtggt
     1261 cttgccgttg gtgccggtga cacccaggca gcgcaccgcg gccgacggga tgccgtagag
     1321 gcgggcggcg gccggcccga gggcgcgccg gacgttcgcg acgcgcgctt cggggacccc
     1381 gaggtcgagc gggcgttcga cgaggagcgc gacggctcca gcgtcgacgg cgctcggggc
     1441 gtgccggtgc ccgtcggtgg cgtgacccac gatgcacgcg aagcaggcgc cgggttcgac
     1501 ccggcggctg tcgtgctcga gggtgatcac ctcgaccctg gcgtcgccgc gaacatcgac
     1561 gtcgacgtcg gccaggagct cctggagttg cacgcgcgcg gtcctttggt caaacaccgg
     1621 gaggcgttca gggtatcggg ccggtcgcgg cggacgatga ggggctggag gcggggacac
     1681 gctcgaccgt aagtgcgtac tgcatcatct tggcgaacgc cggcgcggcg acctccccgc
     1741 cgtagacggc accttggggc tcgtcgagga cgacaacggc ggcgaggcgc ggcgcgtccg
     1801 cgggcgcgaa cccggcgaac gacgcgacgt agttgtacgg cggtttgagg tacggcggct
     1861 tgcgggcggt gccggtcttg ccggcgacgc ggtacccaga gatctgggct tgtgttccgg
     1921 tcccgccggc gacgacgcct tcgagcatct gacgtacctg ggccgcggtg ttcggtgaaa
     1981 cgacggcgtg atcggcgacg gacgtgacct ggtggcggtg gccggcggcg tcgagggtgg
     2041 acgtgacgag ccgcggcggg tgagcgaccc cgttgttggc gatcgtcatg tacacgtcga
     2101 ggagctgcat ggcagtgacc gcgatgccgc tgccgatcgg catcgacgcg aggctcgtgg
     2161 cgttgtactg cgcccgcggg aggaggatcc cggcggcctc gccgggatag ctggtgccgc
     2221 tgggcgcccc gaatccgaag gcgtggatgg ccgcgtcgaa gcggtcccgg cccagcatcc
     2281 gggcgatctc gatcgtcccg acgttggagg actggcggac gatgtcggcg accgtcatca
     2341 acgtcgggtg agtgtcgaca tcttcgtact tggtgttgtc gacggtgatt gactgcggca
     2401 ccgacaggac cgtggacggg gtgacgaggc cggcttcgat cgcggcggcg atggtcacga
     2461 ctttggccgt ggagcccggc tcgaacacgt cggtgagcgg ccggttcggc gacaggccgg
     2521 acgacgggtg ggccgggatc gcgccgttgg ggccgtcgac gctgaccatc gccaagatgt
     2581 tcccggtacg gacgtcggtg aggatcgcca tgccgccctt ggcgtgcgcg cccgcgacct
     2641 cgtcggcgag gacccgctcc gcctggtact gcatcgactg gtcgatggtg aggaacaggt
     2701 ccgatccttt gagctcggga cggtcctggc gagtcccgtt cggcaggtca cggccgcggg
     2761 gatcttgttc gacgacgagt cgaccgttct ggcccgcgag ggcggtttgc gacccggcct
     2821 cgagaccgga caggaccgcg ttgtcggttc cgacgacgcc gaggaccggc gcggcgagcg
     2881 ggtcggccgg gtagtagcgc ttcggctccg gcgtgtagtc gaggccgatg agatgcaggg
     2941 cgcgcaccgc ccgctcgacc ggcgggtcga ctttgcgggc gatgtacacg aagccgtggc
     3001 gatggtcgct caagcgggcc tgcagcgtcg ccgggtcgac gccgacgatg ggcgccagct
     3061 tggcggcgta cgccgccggg tctcggatga cccgcgggtt ggcggagatg ctggtggcgt
     3121 cgaccgacac cgccagcgcg ttgccgttgc ggtcaaagat gctgccgcgc tgggcgggga
     3181 tcgtcaccgt gcggactcgc tgactgagac cgaggcgaac gtatcgaccc cggtttcggg
     3241 cctgcacgtc gacgaggcgg atcccgatca gcgcgaacac gagcacggtc acggtcagga
     3301 gggcggcgag gcgccgtggt gatgaggccg cgctgggggg cagacgatgg tggacccggc
     3361 gtcgcgtcac tggtcggggg gcgtggcgcg cgcgcgtagc gaccgccggc cgagccggcg
     3421 gtcgctcgat ccgcggggct cgcgagcccg gacgggcgcg ggcccgggtc gtcatcgaag
     3481 gctcatggtt gcgaggcctg acgggctgaa actggagcga ccgcgttgct cggcacgggg
     3541 accgggaccg gcggcgcggc gggtgggacc aggccgagca gcagtgctcg ggccgcgatc
     3601 cgcgacggtg aggagagctg cgcgttgacg agacgagcct ggtcgtagcg gcgttgggcg
     3661 tcgtcgacct ggtggctgac gtggtcgagg gtgagctgcg actgggccgt catgacctgg
     3721 aacgtgacga gggcgaggac cgcgaggacg gttcccaccg cggccaggag taccgagcgg
     3781 tgaacccggc gggcccgccg cgcgggggcg acgacacgaa ggtggtcacg gcgcggcggg
     3841 gcgacccggc ggggcgcggg cgcagcggcg ctacgacggg acgcggtgcg gacccgggcc
     3901 gggggtgcgg cgacggccac gggactcatg gtcccgtcgg tgacggcagc ttctcgatgg
     3961 ctcgcaggcg ggcgctctcg gcccggggat tggccgccac ttcgtcagcc tgggggcgaa
     4021 gcgctcgccg ggtgaggagg cgggccaggg cgttgggacg ggtgggttcg accggaagcc
     4081 cgggcggcgg ctggctcgcc gggtcttcac cggcccagcg ggtgaaccgc tccttcactg
     4141 cccggtcttc gagcgagtga taggcgagga cgagcacccg accctggggt ccgatgatgt
     4201 gcaccgactc gtcgaggccg gcagccagat tggggagctc gcggttgacc tccatgcgga
     4261 tggcctggaa ggtccgacgg gccgggtggc cgccgcggcg gcgggcccgg gccgggatgg
     4321 cctccttcac cgcggtgacg aggtcccccg tcgtgtgcag cggccggtgc gcgacgatgc
     4381 gcgacgcgat ggaacgggcg taacgctcct cgccgttgtt ggcgatgacc tcggccaggc
     4441 cggtctcggt gtactcgttc acgacacgct ccgcggtcag cgcctgagcg ctgtccatgc
     4501 gcatgtcgag cggcgcgtca ccccagtagg tgaacccccg ttcggggagg tccaactgcg
     4561 ggctgctgac gccgaggtca aacaggattc ccatgacgtt tccctcgctt gcttgtccca
     4621 cgatctcggc gacgtcttcg aagccgccgt gcacgatccg tgcccggtta ccgaagggtt
     4681 cgagctcggc tcgggccgcc gcgactgccg caggatctcg atcgatcccg aggaggcgga
     4741 ggtccggtcg agcctccagg agccggcggg catgcccacc gccaccgacc gtcgcatcca
     4801 cgacggtccc tgttggcacc ggggcaagga gctcgatcac ctcccggacc atgaccggtt
     4861 ggtgctggaa gccacccatg tgaactcctc gccgacccgg ggcccgtcag cagccccccg
     4921 gacggacgac gtccgttcgg atttctcccc gaagcggtcc ggtggagcgg gcggagtagg
     4981 ggcttcaccg cgccggccgg ggggctgctg acgggccatg gggccacgta cgtctaggtc
     5041 gtttctcctc tctctcgtcc tatatgcccc agctctggcg gcgagcgagt tcggcttggc
     5101 cttcccgctc gacttgctgc cagctctggg gggaccagag ctcgattcgc ttgtcctgac
     5161 cggtgacgat cacctcgcgg tccaggtgtg cccagtcgcg caggtacgcg gcgaccggga
     5221 cccggccttg cttgtcgggg gtgaccgctt gggcgctggc gaagaacacc cgcgaggcgt
     5281 ggacggcggc ttcggtgcca ccttccttcg cgctctggct gatgtcctcg ctcacttcgc
     5341 tgaacctttc tggggtgtag atggccagac agccgcccgg gaactggccg atataggcgc
     5401 cctcttcgag cgccttgcgg aagtccgccg ggaggatgag gcggcccttg tcgtccagcg
     5461 tgtgctggta ccgcccgatg aacgccgttg ccaccatgtc ccctcgccgg cggagcctcc
     5521 cggtgccacc ccggatactc cacaatcaac cactgcgctc caccctatgc ccccagtccc
     5581 cacctcgtca accccttctg cccca
//
LOCUS       DATCCH010000368         4148 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_601611, whole genome shotgun sequence.
ACCESSION   DATCCH010000368 DATCCH010000000
VERSION     DATCCH010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4148
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601611"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1372)
                     /locus_tag="VIJ44_07035"
     CDS             complement(<1..1372)
                     /locus_tag="VIJ44_07035"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY5165686.1"
                     /translation="MTDEPKTSGGSGATRRTESPEPSLVDGYIELREVGRGGFGIVYR
                     ALQVEFNRTVALKVLTNVSDDPGARARFERECLAMGSLSGHPNIVTVYAAGYTIDGRP
                     YIAMEFLPGGTLAERLAGRGISWSEAAKIGIKICGALQRAHDSGVLHRDVKPENVLVS
                     AYDEPKLADFGIARLHGGYETRSGIVTATFAHAAPELLEGKPPSAASDVYSLASTLYS
                     LITGHPPFVSEDEDTLPAVIARIATQPPPDLRGRGVPDPVCAALERALAKDPAARTPT
                     AGEFGRELQDAVRLVGEDPTDMVVAAPDGDGRRRPSTGRGGIRRLGFGSRRTVIAGIA
                     ALALLVGAGVGLVLSLGGGSKSTTPTTTSRIPLAPAMPTKLAGYRKDGAPTSSSLRVF
                     SGRPNYIADFPWTMNGCQGATYTTQWRALTTADLVAAGPTDVHDSTTLTPSAVKGATT
                     GHAGLVT"
     gene            1543..2475
                     /locus_tag="VIJ44_07040"
     CDS             1543..2475
                     /locus_tag="VIJ44_07040"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY5165687.1"
                     /translation="MSESAATVIVVEPGTPEERTVPVSERLFVGRECVGIDKAHRLVL
                     SDDTVSRQHLEIRLDLGEDRAVVLDMSTNGTRVNGVRIERAVPIPIHPGDQLQVGETL
                     LEFRSDRFFSTSQTDRRRTTRYVSQSSMVLVVGDLVGYSTISEVTDSSVVLDSLDVLW
                     DGLRRLLMEHGGTLNSYAGDALFAVWESDPIDDAAAHAADFVRAAQRHVRETAPALTI
                     RTLEGSPVQMGWAVVLGPVAVSTLTRAQPAVIGDTTNLAFRLSGRAARDGLPEILVNA
                     TAREILGDRYEWGEPHTVTVKGRSAEEVVFGLPD"
     gene            2699..3610
                     /locus_tag="VIJ44_07045"
     CDS             2699..3610
                     /locus_tag="VIJ44_07045"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEY5165688.1"
                     /translation="MAITAEPLIPIPAAPEGCSYLHLRVAPIAGACGAVLDGLDLARE
                     LDDEVIAEIRRAVLDHHVVFVREQRLSPERQVAFSRRFGPFSPVPFIEPTPDHPEVIA
                     VVREASERQSFTFGSLWHSDFSFLAEPPFASILHALEVPPYGGDTLWANQELAYSTLS
                     TEMQTMLSSAVGLHSAANAYSPKMQPIHDAFVGMTVRTSDEANRVERHPMVRRHPETG
                     RLALYINAQYTVGLEGFMGHEAKPLLDMLFAHAVRPEFTCRWRWSTGDVAFWDNRCLQ
                     HMVLADYTGHRRVMHRTTVAGDRPVGV"
     gene            3588..3788
                     /locus_tag="VIJ44_07050"
     CDS             3588..3788
                     /locus_tag="VIJ44_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165689.1"
                     /translation="MTGRSGSDQRGGRSRRRATCFDLVVVSALAVGGVLMTPLALPYV
                     LLVLAIAGVVMVLMEPLKVVVL"
     gene            complement(3829..>4148)
                     /locus_tag="VIJ44_07055"
     CDS             complement(3829..>4148)
                     /locus_tag="VIJ44_07055"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEY5165690.1"
                     /translation="RAQLGKPYVYAAAGPDSYDCSGLVMAAYASAGVYLPHYSGAQYA
                     SLPHISMNAMLPGDLLYWGDSGSEHVAIYLGDGRILESGGTGNDVHIGPIWGQPFGAA
                     RPA"
BASE COUNT          629 a         1418 c         1426 g          675 t
ORIGIN      
        1 cggtgacgag tcccgcatgt ccggtcgtgg cgcccttgac cgcgctcggt gtcaacgtgg
       61 tggaatcgtg cacgtcggtg ggacccgcgg ccacgagatc cgcggtggtc agcgcgcgcc
      121 actgggtggt gtacgtggcg ccctgacagc cattcatcgt ccacggaaag tcggcgatgt
      181 agttgggccg gccgctgaag acccgcaggc tcgaactcgt cggcgcccca tccttgcggt
      241 agccggcaag tttggtgggc atggcggggg ccaggggaat cctcgaggtc gtcgtcggcg
      301 tcgtcgactt cgagccgccg ccgagtgaaa ggacgaggcc cacgccggca ccgacgagaa
      361 gcgccagcgc cgcgatcccg gcgatcacgg tgcgtcgcga accgaacccg agccgcctga
      421 tcccgccgcg gcccgtcgag ggtcgacgcc ggccgtcgcc gtcgggcgcg gcgacgacca
      481 tgtccgtcgg gtcttcaccg acgagtcgaa ccgcgtcctg gagctcgcga ccgaactcgc
      541 cggccgttgg ggtgcgcgcg gcggggtcct tggcgagggc gcgctccagc gccgcacaga
      601 cggggtccgg cacgccgcgg ccacgcaggt cgggtggcgg ttgcgtggcg attcgggcga
      661 tcaccgccgg cagagtgtct tcgtcttcgc tcacaaacgg tggatgcccc gtgatcaagg
      721 agtagagcgt cgaggcgagc gagtagacgt ccgacgctgc cgacggtggc ttgccttcca
      781 gcagctccgg tgcggcgtgc gcgaacgttg ccgtgacgat tccactgcgg gtctcatagc
      841 caccgtggag gcgggcgatc ccgaagtcgg ccagcttggg ctcgtcgtag gccgagacga
      901 gcacgttttc gggcttcaca tcccggtgca agacaccact gtcatgagcc cgctggagcg
      961 cgccgcagat cttgatgccg atcttggctg cctccgacca cgagattccg cggccggcaa
     1021 gccgctcggc aagggtccct cccggcagga actccatcgc gatgtacgga cgaccgtcga
     1081 tggtgtagcc ggccgcgtac accgtgacga tgttcgggtg ccccgagagc gagcccatcg
     1141 ccaggcattc gcgttcgaat cgggcacgag cgccggggtc gtcggagacg ttcgtgagca
     1201 ccttcagcgc gacggtgcgg ttgaactcga cctgcaaggc ccgatacacg atcccgaacc
     1261 cgccgcggcc cacctcgcgc agttcgatgt agccgtcgac caggctcggc tccggtgatt
     1321 cggtgcgcct ggtcgcgccg cttccaccgc tggtcttcgg ctcgtcagtc atcgcgtggt
     1381 cctcgccgga tccgacccac cccgctcggc gacgcgaacg gggaggggac ggtcatcgat
     1441 gcgtaccgta ggctccgcac cactggctcg cgactcggca ccaggcgtgc gtgccggtcc
     1501 ggggcctccg ggcccgccgg cccgcgatta gacaggtggc ccatgtcgga atccgccgcg
     1561 acggtgatcg tcgtcgagcc cggaacaccc gaagaacgca cggtgccggt gtccgagcgg
     1621 ctcttcgtcg gacgcgagtg cgtcggcatc gacaaggcgc accgcctcgt gctctctgac
     1681 gacaccgtct cgcgccagca cctcgagatc cgcctcgatc tgggtgagga ccgggcggtg
     1741 gtgctcgaca tgagcacgaa cgggacccgc gtcaacgggg ttcggatcga acgcgccgtg
     1801 ccgataccga tccatcccgg cgatcagctc caggtgggcg agacactcct cgagttccgg
     1861 tctgaccggt tcttcagcac gagccagacc gaccgacgcc gcacgacgcg ctacgtcagt
     1921 cagagctcca tggtcctggt ggtcggggac ctggtcggat actcgacgat ctcggaagtc
     1981 accgacagct cggtcgtctt ggattcgctc gacgtcctct gggacggtct tcggcgattg
     2041 ctcatggagc atggggggac cctgaactcc tacgccggcg acgcgctgtt cgcggtgtgg
     2101 gaatctgacc cgatcgatga cgccgcggct cacgccgccg acttcgtacg ggccgctcag
     2161 cggcacgtcc gtgagaccgc tccggcgctg accatccgga cgctcgaagg gagcccggtc
     2221 caaatgggat gggcggtcgt cctggggccg gtcgcggtca gcacgctgac ccgcgcccaa
     2281 ccggcggtga tcggcgacac caccaatctc gcgtttcgcc tctcggggcg ggcggctcgt
     2341 gacggactgc cggagatcct ggtcaacgcg acggcccgcg agatcctcgg tgaccgctac
     2401 gaatggggcg agccacacac cgtcacggtc aagggccgaa gcgccgagga ggttgtgttc
     2461 ggtctaccgg actagccgcg tcgatcggtc ggcggacgat cctgccgctg tcggagacga
     2521 gggcttcgaa caccgagacg cagagtcgcc catgcgcatc gaatcgtcgg cgctttcccc
     2581 tgtcgtggat accgtccgaa gggacgagac ccagcctggg tcttgaggcg ggaccggccg
     2641 gcgagggtgc cggtgcgccc tcgaacgaag ccgccacctc attccgtatg cttgagtggt
     2701 ggcgatcacc gcggagccgc tcattccgat cccggcggcc cccgaagggt gctcgtatct
     2761 gcacctgcgg gtcgccccga tcgcgggtgc ctgcggtgcc gtcctcgacg gcttggacct
     2821 cgcccgcgag ctcgacgacg aggtcattgc cgagatccgc cgtgccgtcc tggatcatca
     2881 cgtcgtcttc gtacgcgaac agcgactgag cccagagcgc caggtcgcct tcagccgacg
     2941 cttcggaccg ttcagcccgg tgccgttcat cgaaccgaca ccggaccacc cagaagtcat
     3001 cgccgtcgta cgggaggcgt ccgagcgtca gagcttcacg ttcgggagct tgtggcactc
     3061 cgacttctcg tttctcgccg agcctccgtt cgcgtccatc ctccacgcac tcgaggtgcc
     3121 cccatacggg ggcgacacgc tgtgggcgaa ccaggagctc gcgtactcga ccctctcgac
     3181 cgagatgcag accatgctct cgagcgccgt cggactgcac agcgcggcga acgcgtactc
     3241 acccaagatg cagcccatcc acgacgcgtt cgtcggcatg acggtgcgaa ccagcgacga
     3301 ggccaaccgg gtcgagcgtc accccatggt tcggcgccac cccgagaccg gccgtctcgc
     3361 cttgtacatc aacgcgcagt acaccgtggg gctcgagggt ttcatgggac acgaggcgaa
     3421 gccgctcctc gacatgctct tcgcgcacgc ggtgcgcccc gagttcacct gtcgctggcg
     3481 atggtccacg ggcgacgttg ccttctggga caaccgctgc ctgcagcaca tggtgttggc
     3541 cgactacacc ggtcatcgac gggtgatgca ccgcactacg gtcgccggtg accggccggt
     3601 cggggtctga ccagcgtggc ggccggtcac gccgccgcgc gacgtgcttc gacctcgttg
     3661 tcgtctccgc gctcgcggtc ggcggcgtgc tcatgacgcc gctcgcgctg ccctacgtgc
     3721 tgctcgtcct cgccatcgct ggcgtcgtca tggtgctcat ggaaccgctg aaggtcgtgg
     3781 tgctttgacg gctcggcctc gtcggaagcc ggccggggcc agggtcggtc aggccggccg
     3841 ggcagcgccg aacggctggc cccagatggg accgatgtgg acgtcgttgc cggtgccgcc
     3901 ggactcgagg atgcgaccgt ccccgaggta gatcgcgacg tgctcgctcc cgctgtcacc
     3961 ccagtagaga aggtcgccgg gcaacatggc gttcatcgag atgtggggca gtgaggcgta
     4021 ctgcgcgccg gagtagtggg gcagatagac gcctgctgag gcgtaggccg ccatcaccag
     4081 accggagcag tcatacgagt cgggtcccgc ggcggcgtac acgtacggct tgccgagctg
     4141 cgcgcggg
//
LOCUS       DATCCH010000369         2567 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_61944, whole genome shotgun sequence.
ACCESSION   DATCCH010000369 DATCCH010000000
VERSION     DATCCH010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61944"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="VIJ44_07060"
     CDS             <1..569
                     /locus_tag="VIJ44_07060"
                     /inference="COORDINATES: protein motif:HMM:NF024935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY5165691.1"
                     /translation="QLRRFRSGFYRCLTGWADAAFELTDAALCAPAPVSSIPTLSLEP
                     TFHRSRGSLYKALARGQVDAGAMRELLVRHRPRRWPLVFAVDASTWARCDAETSPERG
                     FYYSASKHSAGKPIVAGWSYSWITGLGWAHDSWTAPMDARRIPPGRMPSTSPPPRSAT
                     SSAGSATPSRRRCSASTPATTRSRSPTP"
     gene            844..1002
                     /locus_tag="VIJ44_07065"
     CDS             844..1002
                     /locus_tag="VIJ44_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165692.1"
                     /translation="MAGTLIRVEVEHLPKPTSATKKTLWLWAAGPGFNLDTCWRGYLR
                     RFDIEHTF"
     gene            complement(1474..1740)
                     /locus_tag="VIJ44_07070"
     CDS             complement(1474..1740)
                     /locus_tag="VIJ44_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165693.1"
                     /translation="MIWTLLAVTGAIGVAASVAVGKSRAKARSGLNKPVHHIDPACAA
                     GGHKYQVFDTGYRCATCGNHVSSREGELYGRTEDGRHERRREPR"
     gene            complement(1906..2463)
                     /locus_tag="VIJ44_07075"
     CDS             complement(1906..2463)
                     /locus_tag="VIJ44_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020460478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165694.1"
                     /translation="MAGVLAEAAPILDERARRVLAGAGARQLGRGGIKLVAAAMGALA
                     DTVGKGVAELEAGLVADGRVRVKGAGRPAAERKDPGVWAALDALVDPLTRGDPMSPLR
                     WTTKSTVKLADALTQRGHRNSMKTQGLAGISLYPELRNCPAPSAPRILGIFNDVKRST
                     STPATRACRPSSRNSHRYNSRSWTS"
BASE COUNT          444 a          985 c          749 g          389 t
ORIGIN      
        1 agcagctacg gcgcttccgg tccggcttct accggtgcct gacaggctgg gccgacgccg
       61 cgttcgagct caccgacgca gcgttgtgcg cgccggcgcc ggtgagttcg ataccgacgt
      121 tgagcctgga accaaccttc caccgcagtc gcggcagcct ctacaaggcg ctcgcgcgcg
      181 ggcaggttga cgccggcgcg atgcgtgagc tgctggtgag gcaccgtccg cgcagatggc
      241 cgctggtgtt cgccgtggac gcgtcgacct gggcccgctg cgacgccgag acttctcccg
      301 agcgagggtt ctactactct gcgtccaagc actcggccgg gaagccgatc gtggccggct
      361 ggtcctactc ctggatcact ggcctgggct gggcccacga ctcgtggacc gcaccgatgg
      421 acgcccgccg gatcccccca gggcggatgc cgtcgacgtc accgccaccc aggtccgcga
      481 cctcgtcggc aggctcggcg acaccgagtc gccgacgctg ttctgcttcg acgccggcta
      541 cgacgcgatc gcgctcaccg acaccctgag cgacgttgcc gccgacatcg tggtccggat
      601 ccgcgatgac cgggtcatca cttctacacc gacgccacgc caccggcggc cggcacggtc
      661 gggcggcccc gacgccacgg cgaccggttc aggctcgccg atcccaacac ctggcccacc
      721 cccgacgtcg agtcgaccac taccgacctc cgctacggca ccgtcaaggt ctcggcctgg
      781 cacgggctgc acccacgact cggctgccgg ggacactggg ccgagcacga catcccgccc
      841 atcgtggccg gcaccctgat ccgggtcgag gtcgaacacc tgcccaaacc caccagcgcg
      901 accaagaaga ccctgtggct gtgggccgcc ggacccggct tcaacctcga cacctgctgg
      961 cggggctacc tgcgccgctt cgacatcgaa cacaccttct gagttcctca agaacaccct
     1021 cagctggagg acccccgcgc tgcgcacccc cgaacaagcc gaccgctgga cctggctcgt
     1081 cctcgccgcc tacacccaac tccacctcgc ccgagacctc gtcgacgacc tccgcctccc
     1141 atgggaacga caacgcaaaa ccggccgact cacccccgcc cgcgtccgaa gagggtttcg
     1201 acgacttgcc ccaacactgg gcaccccagc cagtccacca aaatcccgaa cccccggccc
     1261 aggacgcccc aaaggaaccc gccgagagcg acgaacccgc catccagcga tcaagaaggc
     1321 cgcctaatca aggtttaatc gcaagcctag gagaccgtac gagtcttctc cggcgcctcg
     1381 cgagagctga gggcctgggg cccgcgcgga aggtggccgc accgaccctc cccctcgtcc
     1441 ccagcgcttc taagccagcc atcttgtccg tgctcaccgg ggttcgcggc ggcgctcatg
     1501 tcgaccgtcc tcggtccggc catagagttc gccttcacgg ctggatacgt ggttgccgca
     1561 cgtagcacag cggtacccgg tgtcgaagac ctggtactta tggccgccag cggcgcacgc
     1621 cgggtcgatg tggtggacgg gcttattcag cccagagcga gccttagccc gcgacttgcc
     1681 gaccgcgacc gacgcggcga cgccgatagc cccggtgacc gcaaggagcg tccagatcat
     1741 cgtcggcgtc ataaaggtcg gtccgtcacc caaccaccag tagaacccat gccccgacct
     1801 ctcttccggt attccgcaag ggcatcttat ctgacgttcc gcacttctcg cagtcgatat
     1861 agccccccac ttgggggggt caggtgtaga tgcttgctgg gatgtttagg aggtccagga
     1921 cctgctgttg tagcggtgtg agttgcggct ggaaggtctg cacgctctgg tcgccggtgt
     1981 tgaggtggac cgtttgacgt cgttgaagat gcccaggatg cggggagcac tgggcgctgg
     2041 gcagttgcgc agttcggggt agagcgagat accggcgagg ccttgggttt tcatcgagtt
     2101 ccggtgacct cgctgggtga gcgcatcggc cagcttcacc gtcgacttcg tcgtccaccg
     2161 aagcggcgac atcggatccc cccgagtcaa cggatccacc aacgcgtcca acgccgccca
     2221 cacccccgga tccttacgct ccgccgccgg acgccccgcg cccttgaccc gaacccgccc
     2281 atcagcgacc agccccgcct ccaactccgc cacacccttg ccgaccgtgt ccgccaatgc
     2341 ccccatcgcc gccgccacca gcttgatccc accccggccg agctggcgag cccccgcacc
     2401 ggccaacacc cgccgcgccc gctcatccaa aataggcgcc gcctctgcta acacccccgc
     2461 caacgcggca gcgaacgccg caccgaaacc cgccgacttc ctggccactg caacaacatc
     2521 cgtgatctcc gccacagcag aagactagag accattcacg ctcaaac
//
LOCUS       DATCCH010000370         5700 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_353116, whole genome shotgun sequence.
ACCESSION   DATCCH010000370 DATCCH010000000
VERSION     DATCCH010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5700
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353116"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="VIJ44_07080"
     CDS             complement(<1..441)
                     /locus_tag="VIJ44_07080"
                     /inference="COORDINATES: protein motif:HMM:NF015605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5165695.1"
                     /translation="MTDIGWAGLGISASLVAVAVVLSLWRQLGLERSIAWSAIRALAQ
                     LLAIGLVLEVLLEPGVSSAWAWLWVAFMLLIAAWTVARRAPVVPGLFGLSLAACAASA
                     VLTLGVLFGFGVFTVNVRTIVPLAGLMIGNSMAATVLVARRLVEE"
     gene            complement(438..1163)
                     /locus_tag="VIJ44_07085"
     CDS             complement(438..1163)
                     /locus_tag="VIJ44_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEY5165696.1"
                     /translation="MTPLFRFEQVTVDGVRRPRLDGATGTVPEDGITVVAGPSGSGKS
                     TLLRCCNRLELPTSGRVLLRGEDVAAQDPLQLRRRVGMVFQRPTPFPGSVRDNLRVAE
                     PNLGDDDAVRALESVGLDASFLAGAATDLSGGEAQRVCLARTLVTRPEVVLMDEVTSS
                     VDPAARLGLETLARHLAETAVRVVWVTHDLEQLRRLADHVLILIGGRVAHSGPAATLE
                     SDSPPEVRRFLAAVAVDDEVGER"
     gene            complement(1223..1645)
                     /locus_tag="VIJ44_07090"
     CDS             complement(1223..1645)
                     /locus_tag="VIJ44_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165697.1"
                     /translation="MSRGLAARLALAYLAISGLFVGVWASVAPRSFFSDFPGLGHMWT
                     ASDGLYNEHLVRDVGDLNLALVVVTIAACIWFSRSLAIIAALAWIVYSAPHLAYHAFN
                     LDVVSAGDQVAEIVTLTVPILLAGFVIVATRNHPETAG"
     gene            complement(1642..2574)
                     /locus_tag="VIJ44_07095"
     CDS             complement(1642..2574)
                     /locus_tag="VIJ44_07095"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5165698.1"
                     /translation="MKVFLTGATGAIGPSTVRALLDAGHDVRAVARDPEKATRLRDAG
                     AEPVGVDLFDADATRSATEGCDAIAHLATNVPPIARMARRGAWATHNRLRTEATRHLL
                     AAAEANGIDTVVKESVTFVYPDHGDRWIDESVPPDLRTTLLAPTLEGEQLVTDFASTG
                     ARAVVLRFGLFYGPTSRSVDEGLRLARWRGATVAGPPDAYLSSIHSDDVATAVVAALG
                     APSGLYNVVDDHPLTRRDYLDAFGGAFDLPQLRLTPGWVLRLVAGSSAGALTASQRVS
                     NQRFRGATTWEPAYPSVQQGWAAIAESRHAASTR"
     gene            complement(2571..3053)
                     /locus_tag="VIJ44_07100"
     CDS             complement(2571..3053)
                     /locus_tag="VIJ44_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015037744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEY5165699.1"
                     /translation="MTEGVEWGLHCVTLAAVCPPDATLTAGRLAEYHGVPTPYLAKHL
                     QSLSRAGVLDAVPGARGGYRLARPAVAISVLDVVEAIDGSEPSFRCTEIRRQGPARLP
                     AREYRVPCSIHVVMQRADDAWRAELRHTSIADLVGMVAASAPPEALVKGLSWMQEVLR
                     "
     gene            complement(3126..3908)
                     /locus_tag="VIJ44_07105"
     CDS             complement(3126..3908)
                     /locus_tag="VIJ44_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014988770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEY5165700.1"
                     /translation="MSDAVFHLDGDRAVPTVLARGPWSPDAQHGGAPAALLARALERA
                     DPGPAHFVSRLTVELLRSVPLAPLTVRTRTLRPGKRVQWLEASLVVDDQEVARATALR
                     LRTDDTLALPVPEPPPLAVSPVADSVPYAIEFPQSDIGFWKAMELRTAAGSFADVGEA
                     TLWFRLAVPVVAGEEPTPLQRVAAAADFGNGVSAALERGRYLFINPDLTIYLHRHPVG
                     EWVALEARTYAEAHGVGLAESALHDQTGRIGRSLQSLLVDRL"
     gene            3937..4389
                     /locus_tag="VIJ44_07110"
     CDS             3937..4389
                     /locus_tag="VIJ44_07110"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY5165701.1"
                     /translation="MPEHSPKEVEREFQRFVERGSADDWSAWADQFTPDALYIEHAMG
                     TFRGREAIREWITVTMASVSGMTFPVEWHVIDGDRVIMYCWNVFEPLAGMTGDYRFAV
                     VTILEYAGDGQWASEEDIYNEKEAEAVLTRFLEDAAAAGHAPPAGPET"
     gene            4464..5213
                     /locus_tag="VIJ44_07115"
     CDS             4464..5213
                     /locus_tag="VIJ44_07115"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEY5165702.1"
                     /translation="MMLRTWTRLAGVAALAALTVGAAGTGVAHADATTTGPTSQTSHS
                     ITAVGDGAVEGTPNVLELELGVTTRDPSAATALAHNSELAVKVIGALKNAGVADKDVQ
                     TSNLSISPNVSGSGNHVDGYEVGNTVMAKLRDVNKAGDIVDAATKVSSNEIVVQSLSF
                     SFDDNSSMVAAARTLAVKRAQDQARQLADAAGVALGNLDSISSTSVPVGPPTETPGAR
                     ATPSPAAAPTPPVNPGSQTVSVEVTLVYQIG"
     gene            5318..>5700
                     /locus_tag="VIJ44_07120"
     CDS             5318..>5700
                     /locus_tag="VIJ44_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HEY5165703.1"
                     /translation="MLFPTITFAIFFMVVLLTSWLLIRRRLDWQLFMIAASYYFYAYY
                     DWRFAFLLAGSTVANWIVGRQIYRSDNDTTRRLWLVLGVVVNLGALGYFKYEEFFLSS
                     ATNLLHTLGIGVSPTIVSVALPIGIS"
BASE COUNT          891 a         1906 c         2153 g          750 t
ORIGIN      
        1 ctcttcgacg agccggcggg ccaccaggac ggtggcagcc atcgaattgc cgatcatcag
       61 ccccgcgagc gggacgatgg tgcgcacgtt gacggtgaac accccgaagc cgaacaggac
      121 gccgagggtc aggaccgccg aggccgcgca cgcggcgagc gacagcccga acaggccggg
      181 cacgacggga gcccgacggg cgacggtcca ggccgcgatg agcagcatga acgcgaccca
      241 cagccacgcc cacgccgacg acacccccgg ttcgagcaac acctcgagga cgaggccgat
      301 ggccagcagc tgggccaagg cgcggatcgc cgaccaggcg atgctgcgct cgagaccgag
      361 ctgacgccac agcgacagga ccaccgcgac cgcgacgagg ctggcggaga tcccgagtcc
      421 agcccagccg atgtcggtca tcgttcgccg acctcgtcgt cgaccgcgac cgcggcgagg
      481 aagcgccgca cctcgggtgg gctgtcggat tcgagggtcg cggcgggacc cgagtgcgcg
      541 acgcggccgc cgatgaggat cagcacgtga tcggcaaggc gccggagctg ctcaaggtcg
      601 tgagtgaccc acacgacacg caccgcggtc tccgccaggt ggcgggccag cgtctcgagg
      661 ccgagccggg ccgcggggtc gaccgacgac gtgacctcgt ccatgagaac gacctcggga
      721 cgggtgacga gggtgcgggc gagacagacc cgttgcgcct ccccgccgga gaggtcggtg
      781 gccgccccgg cgaggaagct ggcgtcgaga ccgacggact ccagggcccg gaccgcgtcg
      841 tcgtcgccga ggttcggttc cgcgacccgc aggttgtcgc ggaccgagcc gggaaacggc
      901 gtcggacgtt ggaagaccat cccgacccgg cggcggagct ggagggggtc ctgggcggcg
      961 acatcctctc cccgcagcag cacccggccg gacgtgggga gctcgagccg gttgcagcag
     1021 cggagcaacg tcgacttgcc cgaacccgac gggcccgcga cgacggtgat cccgtcctcg
     1081 ggcaccgtgc cggtcgcgcc gtcgagccgg gggcgacgga cgccgtcgac ggtgacctgc
     1141 tcgaagcgga acagcggagt cacagcgaca gcgatgccgc attgtcgcgc cgcttcggac
     1201 ggttccccgg cgcgacgtca tctcagcccg cggtctccgg gtggttccga gtggcgacga
     1261 tgacgaaccc ggccaagagg atcggaaccg tgagcgtgac gatctccgcg acctggtcgc
     1321 ccgcgctgac cacgtcgagg ttgaacgcgt ggtacgccag gtgcggcgcc gagtagacga
     1381 tccaggcgag cgcggcgatg atcgccaggg aacgcgagaa ccagatgcac gccgcgatcg
     1441 tcacgacgac gagcgccagg ttcaggtctc cgacgtcgcg gaccaggtgc tcgttgtaga
     1501 ggccgtcgga ggcggtccac atgtggccga gcccggggaa gtccgagaag aacgagcggg
     1561 gcgcgacgga ggcccagact ccgacgaaga gcccgctgat cgcgaggtac gcgagggcga
     1621 gccgggcggc gagtccgcgg ctcatcgggt cgacgccgcg tggcgggact cggcgatggc
     1681 ggcccagccc tgctggacgc tcggataggc cggctcccag gtcgtcgcgc cgcggaaccg
     1741 ctggttggag acccgttgcg acgcggtgag cgcgccggcc gaggagccgg cgacgaggcg
     1801 gagcacccag cccggtgtga gccgcagctg gggtaggtcg aacgcgccgc caaacgcgtc
     1861 gaggtagtca cggcgcgtca acgggtggtc gtccaccacg ttgtagaggc ccgatggcgc
     1921 gcccagcgcg gcgaccaccg cggtggcgac gtcgtcgctg tggatcgagg acaggtaggc
     1981 gtccggtggg ccggcgacgg tcgcgccgcg ccagcgggcg aggcggagcc cctcgtcgac
     2041 ggagcggctg gtcggcccgt agaacagccc gaagcgcagg accaccgcgc gggcaccggt
     2101 gctcgcgaag tcggtgacga gctgttcgcc ctcgagggtc ggtgcgagca gcgtggtgcg
     2161 gaggtcgggc ggtaccgatt cgtcgatcca gcggtccccg tggtcggggt agacgaaggt
     2221 gaccgactcc ttcacgaccg tgtcgatccc gttcgcttca gccgcggcga ggaggtgccg
     2281 ggtggcctcg gtgcggagcc ggttatgcgt cgcccaggcg ccgcgacgcg ccatgcgtgc
     2341 gatcggcggc acgttggtcg ccaggtgcgc gatggcgtca cagccctcgg tcgcgctccg
     2401 cgtcgcgtcg gcgtcgaaga ggtcgacgcc gaccggctcg gcgcccgcgt cgcggagccg
     2461 ggtggccttc tcggggtcgc gggcgaccgc ccggacgtcg tggcccgcat cgagcagcgc
     2521 ccggaccgtg ctcggcccga tcgcgccggt ggctccggtg agaaacacct tcatcgcagt
     2581 acctcctgca tccagctcag tcccttgacc agcgcttcgg gtggcgcgga cgccgcgacc
     2641 atgccgacga ggtcggcgat ggacgtgtgg cgcagctcgg cgcgccacgc gtcgtcggcg
     2701 cgttgcatga cgacgtggat cgagcacggc acgcggtact cgcgcgctgg gagacgggcc
     2761 gggccctggc ggcggatctc ggtgcagcgg aacgagggct cggagccgtc gatggcctcg
     2821 acgacgtcga ggaccgagat ggcaacggcc gggcgggcga ggcggtagcc gccccgggcg
     2881 cctgggaccg cgtcgaggac cccggcgcgc gagagcgact ggaggtgctt ggccagatac
     2941 ggggtgggca cgccgtggta ctcggcgagg cggcccgcgg tcagggtggc gtcggggggg
     3001 cacaccgcgg cgagcgtgac gcaatggagg ccccactcga cgccttcggt catgcgcact
     3061 tctcggatac taactgtccg ggttagccga cgccgacgtg cgggcgggtg cgagggccag
     3121 cgggatcaga gccggtcgac gaggaggctc tggagggagc ggccgatgcg cccggtctgg
     3181 tcgtggaggg cgctctcggc gagaccgacg ccgtgtgctt cggcgtaggt gcgggcctcg
     3241 agcgctaccc actccccgac ggggtggcgg tgcaggtaga tggtgagatc ggggttgatg
     3301 aagaggtacc ggccgcgttc gagggccgcg ctgacaccgt tgccgaagtc ggcggcggcc
     3361 gcgactcgct gcagcggggt gggctcctcc ccggcgacga ccgggaccgc gaggcgaaac
     3421 cacagcgtcg cctccccgac gtcggcgaac gagcccgccg cggtgcgcag ctccatcgcc
     3481 ttccagaacc cgatgtcgga ctgcgggaac tcgatggcgt aggggacgct gtcagcgacc
     3541 ggcgacacgg ccagcggcgg cggctccggg accggcaggg cgagggtgtc gtcggtgcgg
     3601 agtcgcaacg cggtggcgcg cgccacctcc tggtcgtcga cgacgaggct cgcctcgagc
     3661 cactggactc tcttgccggg gcggagggtc cgggtgcgaa ccgtgagcgg tgcgaggggg
     3721 accgatcgca gcagctcgac cgtcagccgg ctgacgaagt gcgccggtcc ggggtcggcc
     3781 cgctcgagcg cccgggccaa cagcgccgcg ggggcgccgc cgtgctgcgc gtcgggagac
     3841 cagggaccgc gcgcgagcac ggtcgggacc gcgcggtcgc cgtcgagatg gaagactgcg
     3901 tcgctcatcg ccggtggagc gtagaaggtg tgtcccgtgc ccgagcacag ccccaaggaa
     3961 gtcgagcggg agtttcagcg gttcgtcgag cggggctcgg cggacgactg gagcgcctgg
     4021 gccgatcagt tcacccccga cgcgctctac atcgagcacg cgatggggac cttccggggg
     4081 cgggaggcga tccgcgagtg gatcacggtg acgatggcgt ccgtctcggg tatgacgttc
     4141 ccggttgagt ggcacgtcat cgacggcgac cgggtgatca tgtactgctg gaacgtgttc
     4201 gagccgctgg cgggaatgac cggcgactac cggttcgcgg tcgtgacgat cctcgagtac
     4261 gcgggcgacg ggcagtgggc ctccgaggag gacatctaca acgagaagga ggccgaggcg
     4321 gtcctcaccc ggttcctcga ggacgccgcc gcggcgggcc acgccccgcc ggctggcccc
     4381 gagacctgat ccgcccccgg ctggtggtgg gaaccgcgcc gacaccctga gcgtccaagt
     4441 cctcttgaac gaaggaggac gtcatgatgc ttcgaacttg gacccggctc gccggggtgg
     4501 cggcgctcgc ggcactgacc gtcggggccg ccgggaccgg cgtggcccac gccgacgcca
     4561 cgacgacggg cccgacgagc cagaccagtc actcgatcac cgcagtcggg gacggagccg
     4621 tcgaaggcac ccccaacgtg ctcgagctcg agctcggggt cacgacccgg gacccgtcgg
     4681 cggcgacggc gctggcccac aacagcgagc tggcggtgaa ggtcatcggg gcgctcaaga
     4741 acgcgggtgt cgccgacaag gacgtccaga cctcgaacct gtccatttcc ccgaacgtca
     4801 gcggctccgg gaaccacgtc gacgggtacg aggtcggcaa caccgtgatg gccaagctgc
     4861 gtgacgtcaa caaggcagga gacatcgtcg acgccgccac gaaggtctcc agcaacgaga
     4921 tcgtggtcca aagcctttcg ttcagcttcg acgacaacag cagcatggtc gcggcggcac
     4981 ggacgctggc ggtcaagcgg gcccaggatc aggcccgcca gctggccgac gcggcagggg
     5041 tggcgctggg caacctcgac tcgatctcgt cgaccagcgt tccggtggga ccgcccaccg
     5101 agacgccagg cgcgcgtgcc acaccgagcc cagccgcggc tccgacgccc ccggtcaacc
     5161 ccggttcaca gacggtgtcg gtggaggtga ccctcgtgta ccagatcggt tgacgagccc
     5221 tgtcgggcga ccgcggtgag tccggccgca acggcccgat ggatccggtc ggcccaggga
     5281 gtttcgagag ggcgaggcgc gagactgtcc ggcgcccatg ctcttcccga cgatcacctt
     5341 tgccatcttc ttcatggtcg tgctcctgac cagttggttg ctgattcggc gccgcctcga
     5401 ctggcagctc ttcatgatcg cggcgtccta ttacttctac gcctactacg actggcgttt
     5461 cgcgttcctg ctcgcgggct cgacggtcgc caactggatc gtcggccgcc agatctaccg
     5521 gagcgacaac gacacgaccc ggcggctgtg gctggtcctc ggtgtcgtcg tcaacctcgg
     5581 ggcgctcgga tacttcaagt acgaggagtt cttcctcagc tcggcgacga acctgctcca
     5641 caccctcggg atcggtgtgt caccgacgat cgtgtcggtg gcgctgccga tcgggatctc
//
LOCUS       DATCCH010000371         2453 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_602382, whole genome shotgun sequence.
ACCESSION   DATCCH010000371 DATCCH010000000
VERSION     DATCCH010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602382"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            441..1313
                     /locus_tag="VIJ44_07125"
                     /pseudo
     CDS             441..1313
                     /locus_tag="VIJ44_07125"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(1360..>2453)
                     /locus_tag="VIJ44_07130"
     CDS             complement(1360..>2453)
                     /locus_tag="VIJ44_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018332881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165704.1"
                     /translation="DVALVAAGAVIVLAVFDSALRTFVLPRGVSTLLTRALFVPLRRV
                     FDVLAREGRSYEARDRVMALYGPLSLFGLVFFWMSLVIAAFTLIYRGGLGVSTWKHAF
                     ELSGSSFFTLGFASTPSSTVGYVLVFVEAASGLGILALLIAYLPTINSTFSRRELAVA
                     KLATRAGTPPSAVELLQRYHAINWNDRLPALWAEWEDWFADISETHTSLAVLVFFRSP
                     NPHRSWVTAAGALLDAASLAQSTLAIPWSPEAGLCIRSGALALREVAQFFAIPYDPDP
                     APNDPISIARSEFDEAYEALGGAGVPVRPDRERCWRDFAGWRVNYDSVLLALAGLTMA
                     PYAPWSSDRSLRFRRRVLRVRPARRRAPS"
BASE COUNT          339 a          884 c          896 g          334 t
ORIGIN      
        1 cctggccatg ccgctcgagg tgttccgggc cgcgttccgc tacgagtggt tcattcggcg
       61 tgatgcagca cccgacaccc gcgagacgtc cctgctcgac ccgtgacggc tgatcggtac
      121 cttccggacg ggggacgccg cggcccgctc ggtcgccgcc gcggtctcgg gcccgcccgg
      181 gcgcgatgag ctgggcgctg ctcggattcg ggctcgtcgc ggcggcgttg acgatcccgg
      241 cggtccgtac ggtgcgggtg tggctcgtcc tcttccccgc gttcgtcatc agcctcgtga
      301 ccgccgagct tcctggctgg tgcctcaccg tcaccgtcgc cggggccgca gccctcgtcg
      361 gggtcggggg cctcgccggc ggggccggct gggtcgggct cggcgcctcc ggcgtcgccg
      421 tgggagggct ggtccgccag atggtcctgg cccgacgggc cgcccccgtc gtggacgaga
      481 acctcggcgc gctcgacggc cgccatcggc cgccgccgcg gcggtcggcc acggaccggc
      541 tcaacccgtt ccggctcggt gaccgcgacg tcgttcgggt cgacgaggtg cggtacgcgc
      601 cgggcgccgg ccggcgtcac ctcctcgacg tctaccgcgc ccggagcggg acggatggcg
      661 cgccggtgct gctccagatc cacggtggcg catggatcag cggcgggaag cggacccaag
      721 gccggctgct catgaaccgc ctggcccggg caggctgggt gtgcgtcgcg aacaactacc
      781 agctgagccc cgcggtgaag cacccggagc acctggtcga ctgcaagcgg gcgttggcgt
      841 ggatccgcga gcacatcgcc gagtacggcg gcgacccgac ccgcgtcgtc gtcaccggcg
      901 ggtcggctgg cggtcacctc gcggcgctcg tggccctcac cgccaacgat ccggcgttcc
      961 agcccggctt cgagtccgtc gacacgtccg tgcttgcctg cgtctcgatg tacggcgcgt
     1021 attcgctcac cgagctgttc gcctggaccg ggcgggcaca gcggatcgcc gcgtggatgg
     1081 gccgcctcgt cgcgggagtc gacgtcgcga ccgctcgcca cgtgttcgat cgggcgtcgc
     1141 cgctccaagc cgtgcaccgg ggcgccccgc cgtttctgat catgcacggc accgtcgaca
     1201 acctcgttcc ggtcgagcaa gcccgccggt tcgtcctcgc gcttcgcacc gtggtgccgg
     1261 acaccgtcgt gtacgtcgag ctccccggcg cgccgcacgc gttcgacgtc gtgcgatcgg
     1321 tccgaaccac cgcggctctg gagggggccg agcggttcgt cagctgggtg ctcgacgccg
     1381 tgccggacgg acgcgcagca ccctccgccg gaaccgcaac gagcggtccg acgaccacgg
     1441 cgcgtacggg gccatcgtca gacccgcgag ggcgagcagc accgagtcgt agttgacgcg
     1501 ccaccccgcg aagtcccgcc agcagcgctc ccggtcgggg cgcaccggga cgcccgcacc
     1561 gccgagcgcc tcgtaggcct cgtcgaactc ggagcgggca atgctgatcg ggtcattcgg
     1621 tgccgggtcg gggtcgtacg ggatggcgaa gaactgggcg acctctcgga gcgccagcgc
     1681 ccccgagcgg atgcagagcc cggcttccgg tgaccacggg atcgccaggg tcgactgggc
     1741 gagcgacgcc gcgtcgagca gggccccggc cgccgtgacc caggaccggt gcgggttcgg
     1801 ggaccggaag aacacgagca ccgccagcga ggtgtgcgtc tcggagatgt ccgcgaacca
     1861 gtcctcccac tccgcccaca gcgccgggag ccgatcgttc cagttgatgg cgtggtaccg
     1921 ctgcagcagc tcgacggcgc tcggtggggt gcccgcccgg gtcgcgagct tcgccaccgc
     1981 gagctcgcgg cgcgagaacg tgctgttgat cgtcggcagg tacgcgatga gcaacgccaa
     2041 gatgccgagt cccgacgcgg cctcgacgaa gaccagcacg tacccgacgg tgctgctggg
     2101 cgtcgaggcg aaccccaggg tgaagaacga ggatccgctc agctcgaagg cgtgcttcca
     2161 cgttgagacg ccgagcccgc cccgatagat cagggtgaac gccgcgatca cgagcgacat
     2221 ccagaagaac accagcccaa agaggctgag cggcccgtag agggccatga cccggtctcg
     2281 ggcctcgtag ctgcggccct cgcgggcgag gacgtcgaac acccgacgga gcgggacgaa
     2341 cagggcgcgg gtgagcaggg tgctcacccc ccggggcagg acgaaggtcc gcagcgcgga
     2401 gtcgaagacc gcgagcacga tcacggctcc ggccgcgacc agcgcgacgt cgg
//
LOCUS       DATCCH010000372         2464 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_395567, whole genome shotgun sequence.
ACCESSION   DATCCH010000372 DATCCH010000000
VERSION     DATCCH010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395567"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="VIJ44_07135"
     CDS             complement(<1..808)
                     /locus_tag="VIJ44_07135"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEY5165705.1"
                     /translation="MPELPQMQALAERLHADLAGRTLTGSEPLGFAGLKTVTPSPDEL
                     IGRTLRAVGRRGKYLVLDFADEEAPSPRLLIHLSQAGRLDLEHPPKRNRGGKGGVVRL
                     RFGGDEQPEAAVLVREHGTERKAAWWVLAAGDDGPLARLGPEPDDDDFAELVLHGTDR
                     RRVHTLIRDQSTVAGVGRGYADDALWRAHLSPYATLAGLNDAQRQRLLDAVRAVLHDG
                     LDRERTRTGGLSEPRLGGHFEVHGRSGTPCPRCGDPMRRVSYDSYEVTYCP"
     gene            complement(859..>2464)
                     /locus_tag="VIJ44_07140"
     CDS             complement(859..>2464)
                     /locus_tag="VIJ44_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEY5165706.1"
                     /translation="DTPAAATASPSPDVAAFAFTAAGPWVIEPGQLRWNEGLDLVRAQ
                     TRADVPRLLEPHRLPPAGRLLRSVASLARALSVWYVRERGTSTSQAGLSRRLRRSFER
                     LGSTYVKLGQIISAFEGLFPDELVTEFKKLRDQVPPEPFAAIRAVVEEDLGRPLDEVF
                     AAFDREPIAAASIAQVHGARLVTGEEVVVKVQRPQVAALMRADVRALAWLAPHLVGRI
                     PVSALANPPALIELFAEQIVEELDFRLEAENMLDLARVFAETEQRAMVVPRPHPTLVT
                     PRVLVMERLRGFAFDDVASMHHAGIDTTAVLRAGLIACLEGAMLYGVFHGDLHSGNLL
                     VEADGRTVLFDFGITGRLAEPQRVAFLRLLLTGTAGDVKGQIAALRDLGAFPSDTDLD
                     AVFADLELDQPVKDPTQMSATEMVDELQELVKKLLGYGARAPKELMLFVKNLMFLNAA
                     TAALAPDLDLIEEIVHIHQYFITHHGDRLARELGLDPTAARVDVDAIKAGFFVPADVD
                     RLTFADLQERRRTILKRMGTKPRRRR"
BASE COUNT          361 a          802 c          930 g          371 t
ORIGIN      
        1 ccgggcagta ggtcacctca taggagtcat aggacacccg ccgcatcggg tccccgcacc
       61 gggggcacgg cgtcccgctt cgtccgtgga cctcgaagtg accgccgagc ctcggctccg
      121 acagcccgcc ggtgcgcgtc cgttcccggt cgagcccgtc gtggagcacg gcgcggaccg
      181 cgtcgaggag tcgttggcgt tgcgcatcgt tgaggccggc gagcgtggcg tacggggaga
      241 ggtgggcccg ccagagcgcg tcgtcggcgt agccgcgtcc gacgccggcg acggtggact
      301 ggtctcggat cagggtgtgc acccgacggc gatccgtgcc gtggaggacc agctcggcaa
      361 agtcgtcgtc gtcgggttcg ggtccgagtc gggcgagtgg cccgtcatcg ccggcggcca
      421 gcacccacca tgccgccttg cgctcggtgc cgtgctcgcg gaccaggacc gccgcctcgg
      481 gctgctcgtc gccaccgaat cggagtcgca cgacgccgcc cttgccgccc cggttgcgtt
      541 tgggggggtg ctcgaggtcg agtcgtcccg cctgggagag atggatgagg aggcgcggcg
      601 acggcgcctc ctcatcggcg aagtccaaga ccaggtactt gccgcgtcgt ccgacagctc
      661 gcagcgttcg gccgatcagc tcgtcgggtg acggggtgac cgtcttgagg ccagcgaacc
      721 cgaggggctc cgatccggtg agggtccggc cggcgaggtc cgcgtggagc cgctcggcga
      781 gcgcctgcat ctggggaagt tcgggcaccc cacgattctg tcgcgccgct gggtgagccg
      841 acggccgacc gctcggcgtc agcgacgacg acggggcttg gtgcccatcc gtttcaagat
      901 ggtgcgacgc cgctcctgga gatccgcgaa ggtgaggcgg tcgacgtcgg cggggacgaa
      961 gaacccggcc ttgatggcgt cgacgtcgac gcgggcggcc gtggggtcga ggcccagctc
     1021 gcgggccagc cgatcgccgt ggtgggtgat gaagtactgg tggatgtgga cgatctcctc
     1081 gatgaggtcg agatcggggg cgagggcggc cgtggcggcg ttgaggaaca tgaggttctt
     1141 gacgaacaac atcagctctt tcggcgcccg ggcgccgtac ccgaggagct tcttgacgag
     1201 ctcttggagc tcgtcgacca tctcggtggc cgacatctgg gtcgggtcct tgaccggctg
     1261 gtcgagctcc aggtcggcga agaccgcgtc gaggtcggtg tcggacggga aggcaccgag
     1321 gtctcgcagc gcggcgatct gacccttgac gtcaccggca gtcccggtga gcagcagacg
     1381 aaggaacgcc actcgctgcg gctcggcgag tcgcccggtg atcccgaagt cgaagaggac
     1441 ggtgcggccg tccgcttcga cgaggaggtt cccgctgtgg aggtcgccgt ggaagacgcc
     1501 atagagcatg gcgccttcca ggcaggcgat cagcccggcg cgcagcaccg cggtggtgtc
     1561 gatgcctgcg tggtgcatcg acgcgacgtc gtcgaacgcg aacccgcgga ggcgttccat
     1621 gacgagcacc cgtggtgtca ccagcgtcgg atgcggccgg gggacgacca tggcccgctg
     1681 ctcggtctcg gcgaacacgc gcgccaggtc gagcatgttc tccgcctcaa gccggaagtc
     1741 gagctcttcg acgatctgct ccgcgaacag ctcgatcagc gccggggggt tggcgagcgc
     1801 cgagaccggg atccgtccga cgagatgcgg cgccagccac gccagcgcgc gcacgtcggc
     1861 gcgcatcagc gccgcgacct gcgggcgttg gaccttcacc accacctctt cgccggtcac
     1921 gagtcgggcg ccatggacct gggcgatcga cgcggcggcg atcggctcac ggtcgaaggc
     1981 ggcgaagact tcgtcgaggg ggcggcccag gtcttcttcc acgacggcgc ggatcgcagc
     2041 gaagggctcg ggaggcacct ggtcccgcag cttcttgaac tcggtgacga gctcgtcggg
     2101 gaagagccct tcgaacgccg agatgatctg gcccagcttc acgtacgtgg acccgagccg
     2161 ttcgaacgaa cggcgcaatc gccgtgacag accagcctgg gaggtgctcg tgcctcgctc
     2221 ccgcacgtac cagacactga gggcccgggc gaggctcgcc accgagcgga gcagtcggcc
     2281 cgccggcggg agccggtgcg gttcgaggag ccgagggacg tcggctcggg tctgggcccg
     2341 gacgaggtcg agaccctcgt tccagcggag ctgtcccggt tcgatgaccc acggtccagc
     2401 cgccgtgaac gcgaacgcgg ctacgtcggg tgacgggctc gcagtggctg cggcgggggt
     2461 gtct
//
LOCUS       DATCCH010000373         4179 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_20998, whole genome shotgun sequence.
ACCESSION   DATCCH010000373 DATCCH010000000
VERSION     DATCCH010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4179
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20998"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..531)
                     /locus_tag="VIJ44_07145"
     CDS             complement(100..531)
                     /locus_tag="VIJ44_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY5165707.1"
                     /translation="MARQLSSADYEQLLRFRTGLRRFLRWSEEEATAAGLTPAQHQLL
                     LAIRGHADAAAPTIGDVADALLLRHHSAVGLVDRAEAAGLVRRRVDRDDARVVRLALT
                     PAGARRLAHLTNAHLEELAVLASTLGPLSEVLTERGYLRDA"
     gene            702..1580
                     /locus_tag="VIJ44_07150"
     CDS             702..1580
                     /locus_tag="VIJ44_07150"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEY5165708.1"
                     /translation="MRIGLFLPSVSPIATPEYLTAYAEAAEAAGFDSIWIGEHVVFLD
                     EYTSQYPYAADGRLALPADNGMLELFTTLTYLAAVTSRVRLGTAVCLVPQRNPVYTAK
                     EVANVDWLSGGRVDFGIGIGWLREEFEVLDAPFESRAAATRDYVEVMKTCWRDDVSTY
                     EGAHYTLPPCRMYPKPVQHPHPPLYFGGETDAALRRVAALGDGWHGFNHLPDSAAMSI
                     RRLEKFLADGGRTRADIDVTVGAYLQPVQPSHLPAYRDAGVDQLVLTGFALDAPGARD
                     VIAKLGDEFIGPASEL"
     gene            1612..1932
                     /locus_tag="VIJ44_07155"
     CDS             1612..1932
                     /locus_tag="VIJ44_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165709.1"
                     /translation="MSQRQLGDVATTKLFENDRVRVWEMQLEPGESSNVHEHQLDYVL
                     IQLQGDKIAGIFEPETKGAYPAGTVEGDVAPGNVIYIGRGGIETAKNTGQQPYREILV
                     ELKD"
     gene            1929..3020
                     /locus_tag="VIJ44_07160"
     CDS             1929..3020
                     /locus_tag="VIJ44_07160"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY5165710.1"
                     /translation="MSPAGSPEAPLTGLRVVELATLYSAPQVGAMLGDLGADVVKVEP
                     PGGDPMRRMGVIRDGVSRNWSWVARAKRSIMLDLDATNDRATFGDLVSAADVLIENLT
                     PGVRSRWNCTFDALAAVNPRLVVVAVSCYGLTGPYADRPGAGTLAEAFGGLTHLTGEP
                     DGPPMLASVPLGDTLTAFSGVIATLAACWDRDRGGGLGRLVDVSMYEPILQVLGGTIA
                     GYDPDDPPARSGSRVRGGAPRNVYRTGDGAYVAVSGTTDPQVARLLVLLDRDGPADRE
                     RYGRAETRVARADELDALVAAWIAARDRETVVDAFLRARIPVAPVNDVGAVLTDPHVA
                     ARGSVEPMLSARGPDLDSGRAAILRDWLG"
     gene            complement(3040..>4179)
                     /locus_tag="VIJ44_07165"
     CDS             complement(3040..>4179)
                     /locus_tag="VIJ44_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020557807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HEY5165711.1"
                     /translation="FGEHWTKGADDLLIDSTTEALQSAGVDKHDVDAFWLGTAMSGMS
                     GMTLSRPLELQNKPVTRVENFCATGSEALRNAAYAVASGAYDIAMAVGVEKVKDSGYQ
                     GLVGAGKTPTDGTNRTLTAAAMFAMCAPAYGTKYGVSDEQMREVLARISWKNHYNGAR
                     NPRAQFRKEVTMETICAAPLMAGGLRMFDCSGVADGSAAAVLVRAEDASKYTDKPLYV
                     KALSFVAGNASGNADPGYDYTWFPEIHAAGQDAYAQAGVTDPRGQIAMAEVHDCFTPA
                     ELLIYEDLGFAERGTAWKEVLAGTFDLAGDLPVNPDGGLKSFGHPVGASGLRMFFEAW
                     LQLRGEAPDERRITTADRGLALTHNLGGYPGEMVSFVGIVGSQPG"
BASE COUNT          621 a         1561 c         1384 g          613 t
ORIGIN      
        1 cgcccggcac cggcatcacc ggcgcctaac cgacgggcac ccgcccgcat cggtgccgcc
       61 gaggccaccc ccaaccgaac gaccgtcggg tacctccgct cacgcgtcgc ggaggtaccc
      121 gcgttcggtg agtacttcgg aaaggggccc cagcgtcgag gccagcaccg cgagctcctc
      181 gagatgcgcg ttggtgaggt gcgccagccg ccgggccccc gcgggtgtta acgcgaggcg
      241 caccacccgc gcgtcgtccc ggtcgacgcg tcggcgcacc agtcccgcgg cctcggcccg
      301 gtcgacgagc ccgaccgcgc tgtggtggcg caagagcagc gcgtcggcca cgtcaccgat
      361 cgtcggggcg gccgcgtcag cgtggccgcg gatggccaag agcagctgat gctgcgccgg
      421 ggtgagcccg gccgcggtgg cctcctcctc gctccagcga aggaaccggc gtagaccggt
      481 ccggaagcgg agcagctgct cgtagtcggc ggagctgagc tggcgcgcca tcaccagatg
      541 gtcccacggc gccgcacggt cgcgacgaga agccccgacg tgagcccgac ccgatcctct
      601 cccccgtgac ccgggcgcgc cgtcatcagc gacaccgcgt cgagggccct atgctcccgc
      661 ggccggctcc ggcacccgcg gtctcgcgcc aggagggcga cgtgcgcatc ggcctgttcc
      721 tgcccagcgt gtctcccatc gcgacgcccg agtacctcac cgcgtacgcc gaggccgccg
      781 aagccgccgg gttcgattcg atctggatcg gggagcacgt cgtgttcctc gacgagtaca
      841 cctcccaata cccctacgcc gccgatggtc gactggctct cccagccgac aacggcatgc
      901 tcgagctgtt caccaccctc acctacctcg cggcggtcac cagccgggtg cgtctcggca
      961 ccgcggtgtg cctggttccc caacgcaacc cggtgtacac cgccaaagag gtcgccaacg
     1021 tcgactggct ctcgggcggt cgggtcgact tcggtatcgg gatcggctgg ctccgcgagg
     1081 agttcgaagt cctcgacgca ccgttcgaat cccgagccgc ggccacccgc gactacgtgg
     1141 aggtcatgaa gacctgctgg cgtgacgacg tctcgaccta cgagggcgcg cactacaccc
     1201 tgccgccgtg tcggatgtac ccgaagccgg tgcaacaccc tcacccgccg ctctacttcg
     1261 gtggcgagac cgacgccgcg ctccggcgcg tcgccgcgct cggcgacggc tggcacggct
     1321 tcaaccatct gccggactcg gcagccatgt cgatccgccg gctcgagaag ttcctcgccg
     1381 acggtggccg gacccgcgcc gacatcgatg tgaccgtcgg cgcgtatctc caaccggtgc
     1441 aaccatcgca ccttcccgcc taccgcgacg ccggcgtcga tcagctcgtc ctcaccggat
     1501 tcgcgctcga cgcacccggc gcccgcgacg tgatcgccaa gctgggcgac gagttcatcg
     1561 gcccggcgag cgaactgtga tccaacaccg agccaggagg ggaagctcgc catgagccaa
     1621 cgtcaactgg gcgacgtcgc cacgaccaag ctgttcgaga acgaccgggt ccgggtctgg
     1681 gagatgcagc tcgaaccagg cgagagctcc aatgtccatg agcaccagct cgactacgtg
     1741 ctcatccagc ttcaaggcga caagatcgcc ggcatcttcg agccggaaac gaagggcgcc
     1801 taccccgccg ggaccgtcga aggcgacgtc gcgccgggca acgtcatcta catcgggcgg
     1861 ggtgggatcg agaccgccaa gaacaccggc cagcagccgt accgggagat cctcgtcgag
     1921 ctgaaggatt gagcccggcg ggcagccccg aggcaccgct cacgggcctt cgcgtcgtcg
     1981 agctggcgac gctctactcg gcaccccagg tcggcgccat gctcggcgat ctgggcgcgg
     2041 acgtggtcaa ggtcgagccg ccgggtggag acccgatgcg acgcatgggc gtcatccgcg
     2101 acggggtctc gcgcaactgg tcctgggtgg cccgcgccaa acggtcgatc atgctcgacc
     2161 tggacgcgac caacgatcgc gccaccttcg gtgatctggt gtccgctgcc gacgtcctca
     2221 tcgagaacct cacgccaggc gtgcggtccc gttggaactg caccttcgac gcgctggccg
     2281 ctgtcaaccc gcgtctcgtt gtcgtcgcgg tcagctgcta cggcctgacc ggcccgtatg
     2341 ccgaccggcc gggggccggg accctcgccg aggcgttcgg cgggctcacc cacctgaccg
     2401 gcgagccgga cggccccccg atgttggcgt cggtcccgct cggagacacc ctcaccgcgt
     2461 tctcaggtgt gatcgccacg ctggccgcgt gctgggatcg cgaccgcggc ggcggcctcg
     2521 ggcgcctcgt cgacgtcagc atgtacgagc ccatcctgca ggtcctcggc gggacgatcg
     2581 ccgggtacga ccccgatgac ccaccggcac gatcaggtag ccgagtccga ggcggcgcac
     2641 cccgaaacgt gtaccgaacc ggtgacggcg cgtacgtggc ggtgtcgggg acaaccgacc
     2701 cgcaagtggc gcggctgctg gtgctcctcg atcgggacgg ccccgcggac cgcgaacgct
     2761 acgggcgggc cgagacccgc gtggcgcgcg ccgacgagct cgacgccctc gtcgccgcat
     2821 ggatcgcggc gcgcgaccga gagaccgtcg tcgacgcgtt ccttcgggcc cgcatcccgg
     2881 tcgctccggt caacgacgtg ggcgccgtcc tcaccgaccc gcacgtcgcg gctcggggca
     2941 gcgtcgagcc gatgctgagc gcccggggcc cggacctcga ttccggtcgg gccgcgatcc
     3001 tccgagactg gctgggctga ccgggcgcga accggtgcgt cagccgggct ggctgccgac
     3061 gattccgacg aagctgacca tctcgccggg gtagccgccg agattgtggg tcagcgccaa
     3121 cccccgatcc gcggtcgtga ttcgccgctc gtcgggcgcc tcgccccgca gctgcagcca
     3181 cgcctcgaag aacatccgca gccccgacgc gccgacaggg tgcccgaagc tcttgagccc
     3241 cccgtccggg ttgacgggca ggtcaccggc gaggtcgaag gtcccggcga gcacttcctt
     3301 ccaagccgtg ccgcgttcgg cgaaccccag gtcctcgtag atcagcagct ccgccggcgt
     3361 gaagcagtcg tgaacctcgg ccatcgcgat ctggccccga gggtcagtga cacccgcctg
     3421 ggcgtacgcg tcttgacccg cggcgtggat ctcggggaac caggtgtagt cgtacccggg
     3481 gtcggcgttc cccgacgcgt tccccgcgac gaacgacaac gccttcacgt acaacggctt
     3541 gtcggtgtac ttggatgcgt cttcggcccg gaccagcacc gccgcggcgc tcccgtcggc
     3601 gacgcccgag cagtcgaaca tccgcagccc accggccatg agcggcgccg cacagatcgt
     3661 ctccatcgtg acttccttgc ggaactgggc ccgggggttc cgcgccccgt tgtagtggtt
     3721 cttccaggag atccgggcca gcacctcgcg catctgctcg tcgctcacgc cgtacttggt
     3781 gccgtacgcc ggcgcgcaca tcgcgaacat ggccgcggcg gtcagtgtcc ggttcgtgcc
     3841 gtccgtcggt gtcttgcccg ccccgaccag cccctgatag ccgctgtcct tgaccttctc
     3901 caccccgacc gccatcgcga tgtcgtacgc gcccgacgcc accgcgtacg ccgcgttgcg
     3961 cagcgcttcg gagccggtgg cgcagaagtt ctcgacccgg gtcaccggct tgttctgcag
     4021 ctccagggga cgcgacagcg tcattcccga catgcccgac atcgcggtcc cgagccagaa
     4081 cgcgtcgacg tcgtgcttgt ccacgccggc gctctggagc gcctcggtgg tcgaatcgat
     4141 gaggaggtcg tcggcgccct tcgtccagtg ctccccgaa
//
LOCUS       DATCCH010000374         5195 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_21160, whole genome shotgun sequence.
ACCESSION   DATCCH010000374 DATCCH010000000
VERSION     DATCCH010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5195
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21160"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            229..1389
                     /locus_tag="VIJ44_07170"
     CDS             229..1389
                     /locus_tag="VIJ44_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165712.1"
                     /translation="MRTPKRLGCRIWRRGAMGFCLVGVGFVGLLAGAGPAAADTQKVT
                     PITECSWKDPATGMYNSEFGYNNPNATSVTVAVGGQNAFSSGGLPQNVGQPTTFAAGK
                     HDNVFIVTSQQTIQWTLQYTNLNAPGGTACSSNPGAGAFYGTAIVFPILDCSWKDPTT
                     GLYNAQFGYNNPFSTSETVAAGGAQNLFSSGGLASNSGQPSTFMAGQHANVFIETIQS
                     NTNWQLTGKNLTAPGSTTCSANPGSGAYYGSTPLVYPVLECDFVDAGTGKTNSLFGYT
                     NPYSTSQTVAVGGQNNFSGSGIAQNAGQPSSFLGGTNANVFVVTHQGTITWSLTNHNV
                     SAPGTKTCSTNPVPIISGRFGSLVVAGAFAVVGVVVLVVRRRRRLARALGLS"
     gene            1544..1738
                     /locus_tag="VIJ44_07175"
     CDS             1544..1738
                     /locus_tag="VIJ44_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165713.1"
                     /translation="MRSGQSLDVSTTPGPHRLRAALSYLAVGDVGSEPIEIALADDGV
                     AEFLVQPTGSPVFKPGDVRG"
     gene            complement(1870..2748)
                     /locus_tag="VIJ44_07180"
     CDS             complement(1870..2748)
                     /locus_tag="VIJ44_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEY5165714.1"
                     /translation="MQGTFLPFRVVPEYSRLVVFRLGRMTGVKGPGIVGVVPLADRVM
                     KVDLREFYLEIPRQTAITKDNAPISIDFITFFKVVDVQASVVQVADFSGAAQNIAATT
                     LRSVVGDISLDDVLAKRDEINQLLRAKLDEVTERWGVKVTNVEIREITPPPAVQEAMT
                     RQMSAERTRRALVTEAEGDKTAAVLRAEGDKQANILQAEGAKQSNILQAEGEKQAAAL
                     RAEGFALALTNVYEVAKGVDAKTMGLQYLEALKSLGASPSTKFVLPLELSGLLQGVAG
                     YAGDAFRDGVSPARAK"
     gene            complement(2786..3406)
                     /locus_tag="VIJ44_07185"
     CDS             complement(2786..3406)
                     /locus_tag="VIJ44_07185"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEY5165715.1"
                     /translation="MNPNLAFVLFVIGMAGVVFEVLHPGLRLPGLAGLAAFVVSLILF
                     GDLPVQATGVVLIVVAFVLFVIDVKMAARGIAALLGTASLVLGGLYLYDPHVHAARVS
                     RPLLIGLAIALGAFFLIVARAAWRAQGLPVVSGTEALLGESAVVTETLHPTGQVRIKG
                     ESWAATLGDDTSATAPVGAKVVVWATEGLTLMVEPVPKMNGKSDPN"
     gene            3794..4882
                     /locus_tag="VIJ44_07190"
     CDS             3794..4882
                     /locus_tag="VIJ44_07190"
                     /inference="COORDINATES: protein motif:HMM:NF024836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY5165716.1"
                     /translation="MAFTFGLAETSEEKEAVYRFRYSVYVEEMGRYQDTADHANRRLV
                     EPEDDHSLVFFARDGVEVVATARLTWGGDGFSERQIRQYSLQPFLDELAPNLMAVGER
                     AMVEPQHRGSGLLDDLLEHSGTLVEDYDIRIVFGACEPHLLSLYLGMGQRTYADKNIN
                     SAEAGYLIPLVSLPKGADSLVGLGDTSDDPTGVPSCVRRVLSSEGGAVLSPMLMEPSQ
                     YWGEVNEALQELEHRGIGAFDGFSEDEVERCLSRSNIIECDTGDHVLKKGGVARNIFV
                     VLDGTLEVRDGDRVVGVLSRGEAFGEIAFLLERPRGLDVYAATDGARVLSLSEGTLRK
                     MIADDAMVAAKLLFNVSKMLCLRLIKAN"
     gene            4929..>5195
                     /locus_tag="VIJ44_07195"
     CDS             4929..>5195
                     /locus_tag="VIJ44_07195"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEY5165717.1"
                     /translation="MTPEPAQIVIVTGSVVARRETFGEVLAASLEHVRRSRAEPGCIS
                     HAVHQDVEDEQRLVFFERWADRESLDDHFRVPASADFVRTVTRLP"
BASE COUNT          876 a         1752 c         1728 g          839 t
ORIGIN      
        1 acctgccgcc gtgtagcgac gcgcgggcac gcgccggtca ccaggccgat cggtaccgtg
       61 gtgcgggctg ttgcaagcgt gcacagcagc aagacgacgg gatcgcttca ctgcgcgcgg
      121 cacgccggtc tgctggtcgg aatcggtgct gacccgggtc gaatcgttgc ccctcaaggc
      181 tttgacccac cgtgccgata ccacggcgag ggttgggagg agagggctat gcgaacaccg
      241 aaacggttgg ggtgtcgcat ctggcgccgt ggcgccatgg gtttctgcct ggttggtgtg
      301 ggcttcgtgg gcctgctcgc cggcgcgggg cccgcggccg ccgacacgca gaaggtgacc
      361 ccgatcaccg agtgcagctg gaaagatccg gccacgggga tgtacaactc ggagttcggg
      421 tacaacaacc cgaacgcgac ctcggtgacc gtcgccgttg gcggacagaa cgccttctcg
      481 agtggtgggc taccacagaa cgttgggcag ccgacgacct tcgctgccgg caagcacgac
      541 aacgtcttca tcgtcacgag ccaacagacc atccaatgga ccctgcagta caccaacctc
      601 aacgccccgg gcggtacggc gtgctcgtcg aacccgggtg ccggcgcgtt ctacgggact
      661 gccatcgtct ttccgatcct cgactgcagc tggaaggacc caaccaccgg tctctataac
      721 gcgcagttcg ggtataacaa cccgttctcc acttcggaga cggtggcggc cggcggcgct
      781 cagaacctgt tctcgagcgg cgggctcgcg agtaactccg ggcagcccag cacgttcatg
      841 gccggccagc acgccaacgt gttcatcgag acgatccaga gcaacacgaa ctggcagttg
      901 acgggcaaga acctcactgc tcccggcagc acgacgtgtt cggccaaccc cggttctggc
      961 gcctactacg ggagcacccc gttggtgtac ccggtcctcg aatgcgactt cgtcgatgcg
     1021 ggtaccggaa agacgaactc gctgtttggc tacaccaacc cgtattccac ttcgcaaacg
     1081 gtggccgtgg ggggccagaa caatttcagc ggcagcggga tcgcccagaa cgccgggcag
     1141 ccgtcgtcct tcctgggtgg gacgaacgcc aacgtgttcg ttgtgacgca ccaggggacc
     1201 atcacctggt ccctgacgaa ccacaacgtc agcgctcccg ggaccaagac ctgttcgacc
     1261 aatcccgtgc cgatcatctc cggccggttc ggctcgctgg tcgttgctgg ggccttcgcc
     1321 gtggttggag tcgtcgtcct cgttgttcgc cgacgccgcc gactggcgcg tgctctcggt
     1381 ctgtcctgag cggccaaccg gatcggtcac gccggcccgg cccgtgtcgc atgtgcccaa
     1441 ttgtcagctg gtggcgacgg tgcgagtgac ccggccaccg ggtcgggttc gagatgtccc
     1501 gcgtgcgtac acgatcctgt agggagaaca gaaggtcggc agcgtgcgca gcggccagtc
     1561 gctcgacgtc tcgacgacgc cggggccgca ccgcttgcgc gccgccctga gctacctcgc
     1621 cgtcggagac gtcggaagtg agccgatcga gatcgcgctc gctgacgatg gagtcgccga
     1681 gttcctcgtc cagccgaccg ggtcgccggt cttcaaaccc ggagatgttc gggggtgacg
     1741 aacctcctac ctggagctcg ttcccgccgc ggcgacatga gctcctgagc gagaccgagg
     1801 gaaccgctcc gggcgcgctc ggcgatccct tcgcgacctc ggagggactc cggccagatc
     1861 atcgaagggt tacttggccc gggctgggct caccccgtcg cggaacgcgt cgccggcgta
     1921 accagccacc ccctggagca ggccgctcag ctcgagcggc aagacgaact tcgtgctcgg
     1981 tgaggcacca aggctcttca gcgcctcgag gtactgcagc cccatcgtct tggcatcaac
     2041 gcccttggct acctcgtaga cgttggtgag ggcgagggcg aagccttcgg cacggagcgc
     2101 ggcggcctgc ttctcacctt cggcctggag aatgttggac tgcttcgccc cctcggcctg
     2161 gaggatgttg gcctgcttgt caccctcggc gcgcaacact gccgcggtct tgtcaccctc
     2221 ggcttcggtg acgagggcac gtcgggtccg ctcggccgac atctggcggg tcatggcttc
     2281 ttgcaccgcg gggggtggtg tgatctcgcg gatctcgacg ttggtgacct tgacgcccca
     2341 acgctcggtg acttcgtcga gcttggcgcg aagcagctgg ttgatctcgt cccgcttggc
     2401 gaggacgtcg tcgaggctga tgtcaccgac cacggaccgc agggtggtcg cggcgatgtt
     2461 ctgcgccgca ccggagaagt cggcgacttg gacgacgctg gcttgcacgt cgacgacctt
     2521 gaagaacgtg atgaagtcga tcgagatggg cgcgttgtcc ttcgtgatcg cggtctggcg
     2581 tgggatctcc aggtagaact cccgcaggtc gactttcatg acccggtcgg cgaggggcac
     2641 gaccccgacg atgccgggtc ccttcacgcc ggtcatgcgg ccgaggcgga agacgaccag
     2701 acggctgtat tccggaacca cgcggaacgg caggaacgtg ccctgcatcc tggctggtcc
     2761 actcattgcg gtacctccgt gtgattcagt tgggatccga tttgccgttc atcttcggga
     2821 ctggctcgac catgagggtg agaccttcgg tcgcccagac caccaccttc gccccgaccg
     2881 gagcggtcgc cgacgtgtcg tcgccgagcg tcgccgccca gctctcaccc ttgatccgca
     2941 cctggccggt cgggtggagg gtctcggtga cgacagccga ttcgccgagc agcgcctcgg
     3001 tgccgctgac cacgggaagc ccttgggcgc gccaggcggc ccgggcgacg atcaagaaga
     3061 acgccccgag cgcgatcgcc agtccgatca gcagcggccg cgacacgcgc gccgcatgca
     3121 cgtgcgggtc gtacaggtac agaccaccga gaaccagcga ggcggtcccg agcagcgccg
     3181 cgatcccgcg ggcggccatc ttgacgtcga tgacgaacaa gacgaacgcc accacgatca
     3241 ggacgactcc tgtcgcttgg accgggaggt cgccgaacag gatgagcgac accacgaacg
     3301 ccgcgagccc ggccagaccg gggaggcgca gcccggggtg caggacctcg aacacgacgc
     3361 cagccatccc gatgacgaag agcacgaacg cgaggttggg gttcacgtca ccgaccaccc
     3421 tcgtgcgatg ggcgatccga gggtgggcca cagcctggtg cgcacgatcc cacctcagcg
     3481 ccgccgcgcc tgacgacgac actcgcagcg taccgacgaa ctcgcccccg tggacgcggc
     3541 tcgcctcgcc tcgggcccgg gtcagactga cgtcgggatt ccggcgcccg atgggtgcga
     3601 ggccgccccg tcggagcgat gaccggcccc gcgaggcgac ccgggcggcc ccgagcccgg
     3661 gcccggcggc accatccact cgggccaccc aagccgtcgc caccgctcag ggcacggtcg
     3721 gtgcccaagc cgaccgcgga accggcccgg ccgggaggtg acacccgggg gcgactgcgg
     3781 gcagcattcg tcgatggcat tcacattcgg tctcgccgag acgtccgaag agaaggaagc
     3841 ggtctatcga ttccgctatt cggtctacgt cgaggagatg gggcgctatc aggacactgc
     3901 ggaccacgcg aatcgacgcc tggtcgaacc cgaagatgac cacagcctgg tcttcttcgc
     3961 ccgcgacggc gtcgaggttg tcgcgactgc ccggctcaca tggggtggcg acggcttctc
     4021 ggaacgccag atccgccagt acagcctcca gccgttcctc gacgagctgg cccccaacct
     4081 gatggcggtc ggggagcgag cgatggtcga accgcagcac cgcgggagcg ggctgctcga
     4141 cgacctgctg gaacactcgg gaactctcgt cgaggactac gacatccgga tcgtcttcgg
     4201 cgcctgcgaa ccgcacctgc tctccctcta cctcgggatg ggtcaacgga cctacgccga
     4261 caagaacatc aacagcgccg aagccggcta cctgatcccg ctggtttccc taccgaaggg
     4321 ggccgactcg cttgtcggcc tcggcgacac ctccgacgac cccaccggcg tgccgagctg
     4381 cgtccggcgc gttctctcca gtgagggcgg cgccgtcctc agcccgatgc tcatggagcc
     4441 cagccagtac tggggcgagg tgaacgaggc actgcaggag ctcgagcacc gaggcatcgg
     4501 agcgttcgac ggattcagcg aagatgaggt cgaacggtgc ctgtcgagaa gcaacatcat
     4561 cgaatgcgac accggcgacc acgtcctgaa gaagggcggg gtggcgcgca acatcttcgt
     4621 cgtgctcgac gggaccctcg aagtccgcga cggcgaccga gtggtcggtg tcctcagccg
     4681 cggcgaggcc ttcggtgaga tcgccttcct gctcgagcgg ccgcgagggc ttgacgtcta
     4741 cgccgcgacc gacggagcgc gggtgctgag cctcagtgag gggacgctgc ggaagatgat
     4801 cgctgatgac gccatggtcg ccgccaagct cctcttcaac gtctcgaaga tgctgtgcct
     4861 acgtttgatc aaggccaact gatgcccacg cgcgcggccc ccggggccag tcgagggagg
     4921 ggtggcgtat gacgcccgag ccagcgcaga tcgtgatcgt gacgggcagc gttgtggcac
     4981 gtcgcgagac cttcggcgag gtccttgccg cgtccctcga gcacgtccga cgctcgcgtg
     5041 cggagcccgg atgcatcagc catgccgtcc accaagacgt cgaagatgag caacggctcg
     5101 tcttctttga acgttgggcc gaccgtgagt ccttggatga tcacttccgg gtgccggcct
     5161 ccgccgactt cgtccggacc gtcacccggc tcccc
//
LOCUS       DATCCH010000375         5706 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_21239, whole genome shotgun sequence.
ACCESSION   DATCCH010000375 DATCCH010000000
VERSION     DATCCH010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5706
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21239"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="VIJ44_07200"
     CDS             <1..353
                     /locus_tag="VIJ44_07200"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEY5165718.1"
                     /translation="LPMGYEYWPQAAEAAIRHAIDVARTPVYVTENGIGTDDDEQRVR
                     FLRESLEGVARTISDGLDVRGFFHWSMLDNFEWAYGYRMRFGLVAVDRATQARTVKPS
                     ATWLGQVINRNRID"
     gene            418..1113
                     /locus_tag="VIJ44_07205"
     CDS             418..1113
                     /locus_tag="VIJ44_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5165719.1"
                     /translation="MGSVEKGTIVVVDDEANIADLVGLYLEREGFRVLKAPTGDAGLE
                     AFRTHRPRLVVLDVGLPDLDGLEVCKRIRLTSQVPIIFLTARDGEVDRVVGLELGADD
                     YLTKPFSPAELVARVKAVLRRTDGAPGPEVVQAGRVAIDVGRREVRVDDGTVELTTKE
                     FDLLKFLAERPGLALSRQQILDGVWGYDWFGDARTVDVHIAQVRKKLGDAVDIHTVRG
                     VGYRLESMRAADG"
     gene            1182..2579
                     /locus_tag="VIJ44_07210"
     CDS             1182..2579
                     /locus_tag="VIJ44_07210"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY5165720.1"
                     /translation="MFAIAFGVLVVAGIGAVGIARQTASAAAIKDLKQKVKPIATELD
                     TLGRQFRTNRLRGNAQQATRTCQLVTAVLRISSASVVAVNADGNVQEGVAPLLGSACA
                     NQANLALPRDVTASDLNVDQVLVGQQQSGVTGATAFVVDPLTPVGTVTPALVLTNGVD
                     TRPLGRAGPYLFLTGAIALLVAGLVSAYLARRMTRPIAAMRDTAQRIAGGDLSARVGV
                     MGQPDDELSSLAQSLDAMATELETARGHERVFLLSVSHDLRTPLTSIRGYAEAITDGM
                     MADPVEQARAGSIIAAEARRLERLVADLLDLARLDAHQFSLHPRPVDARTVVYDAVAG
                     FVPSARDWGLRLEVVRGDPAPLDTDPERLAQITANLVENALKYAQSTVRVDVDRRDGQ
                     VELRVDDDGPGIPVEDQNRVFQRLYTARDAPGRKVGTGIGLAIVHELAGAMGGEASCE
                     PLDAGGTRFRVTIPA"
     gene            complement(2563..2817)
                     /locus_tag="VIJ44_07215"
     CDS             complement(2563..2817)
                     /locus_tag="VIJ44_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165721.1"
                     /translation="MSTALFVFGYPTSIAVLARWILVVRQQRTRWFVAHELAVGAIVA
                     GWVLRHDTQAVTINASWLAVGTAWYVAAGRRQARAATRGS"
     gene            complement(2814..3692)
                     /locus_tag="VIJ44_07220"
     CDS             complement(2814..3692)
                     /locus_tag="VIJ44_07220"
                     /inference="COORDINATES: protein motif:HMM:TIGR03317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165722.1"
                     /translation="MTVPAFVADRSPRGLIVARGDDATSFLQSLVSQDLGPLGDGDHA
                     PALLLTPQGKLDVAFRILRTGDEWWLDTDADYAPRLLSSLNRFRIRVKVDLDDRTATT
                     GLVSTMGVPGDTVPGGVHPLPADWAGHPGVDLLGPLDAVRAATSTLDSTVERWTPERF
                     EAFRIEAGVPRLGVDIDDRTIPQEAFLERDAVSFTKGCFVGQELVCRIDTRGHVNRYL
                     RGVRIPDGPIPPRGATVVVDGTDRGTITSVAAVPGEARAVGLAMVRREVEPPTPAELR
                     WDTGHVDAELVTVGDR"
     gene            complement(3689..5221)
                     /locus_tag="VIJ44_07225"
     CDS             complement(3689..5221)
                     /locus_tag="VIJ44_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HEY5165723.1"
                     /translation="MTSDDGLRPSRDEFRALAVGHSVVPVWREVLADLETPVSAFVKL
                     VGAEPHGAPGFLLESVEHGQRWGRFSFLGRDPALTLVVRGRQVHVTGGTVPPGVPLDQ
                     GALAALDALLGAYRAPHLDPLPPFHGGIVGYLGYDVVREVERLPAVPPDDQGLPDAVL
                     SVTGHVTAFDHFRQRLYLIENVFLDTEPDVDAAYDRAAARLDALVDDLARPLPYSPSR
                     PPADRLTEIPAVRSTLGGGRYHDAVEVAREHILAGDIFQVVLAQRFDLDDPVDPFDVY
                     RVLRQVNPSPYMYVVRHPEVTLVGSSPEPMVQLLDGRVISRPIAGTRYRGQSDEHDRR
                     LAAELSENPKEQAEHVMLVDLARNDVGRVVDFGTEHVDEFMTLERYSHVMHLTSQVSG
                     QLAAGRTPVDVLRATFPAGTVSGAPKVRAMEIIDELEPVKRGPYAGVVGYVDFSGNLD
                     TAIAIRTMVWGPDGHASVQAGAGIVADSDPADEDLECANKARALLTAAAAARRLTSVA
                     LS"
     gene            complement(5218..5586)
                     /gene="hisI"
                     /locus_tag="VIJ44_07230"
     CDS             complement(5218..5586)
                     /gene="hisI"
                     /locus_tag="VIJ44_07230"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010666888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEY5165724.1"
                     /translation="MSPTATPIPIRDEDLGEIHWDATGLVPAIVQDQASGRVLMLAWM
                     NEASLRRTLDEGRTVFWSRSRQEFWRKGDTSGDRQWLREAYYDCDGDTLLLVVEQEGR
                     GACHTGEYSCFFRSFGPDRS"
BASE COUNT          839 a         1809 c         2223 g          835 t
ORIGIN      
        1 tgctcccgat gggttacgag tactggccgc aagccgcgga ggcggcgatc cgccacgcca
       61 tcgacgtggc gcgcaccccc gtgtacgtga ccgagaacgg gatcggcacc gacgacgacg
      121 agcagcgggt ccggttcctg cgcgaatcgc tcgagggggt ggcccgcacc atcagcgatg
      181 ggctcgacgt gcggggcttc ttccactggt cgatgctcga caacttcgag tgggcgtacg
      241 ggtatcggat gcggttcggg ctcgtcgcgg tggaccgggc cacgcaggcc cgcacggtga
      301 agccgagcgc gacgtggctg ggccaggtga tcaaccggaa ccgcatcgat tgatgatcgg
      361 ggcttcacac gacgctgacc gagacggtga acgctggaga ccactcgggg agtcagaatg
      421 ggcagtgtgg agaaagggac gattgtcgtc gtcgacgacg aggcgaacat cgccgacctg
      481 gtcggcttgt acctcgaacg ggaggggttc cgggtcctga aggccccgac gggcgacgcc
      541 gggctggagg cgttccgtac ccaccggccg cgtctggtgg tgctcgacgt cgggttgccc
      601 gacctcgacg ggctcgaggt ctgcaagcgg atccgactga cgtctcaggt gccgatcatc
      661 ttcttgaccg cccgagacgg tgaggtcgac cgtgtcgtgg ggctcgagct gggcgcggac
      721 gactacctga cgaagccgtt ctcgcccgcg gagctggtgg cccgggtgaa ggcggtgctg
      781 cgccgcaccg acggtgcacc gggcccggag gttgtgcagg cgggccgggt ggcgatcgac
      841 gtagggcgac gggaggtccg tgtcgacgac ggcaccgtcg agctcacgac gaaggagttc
      901 gacctgttga agttcctggc cgagcggcca gggctggcgt tgtcgcgtca acagatcctc
      961 gacggcgtgt ggggctacga ctggttcggg gacgcgagaa ccgtcgacgt gcatatcgcg
     1021 caggtgcgca agaagctcgg tgacgccgtc gacatccaca cggtgcgcgg cgtcgggtat
     1081 cgcctcgagt ccatgcgcgc cgctgacgga tgaggatcag gcgggcctcc cggtccgtcg
     1141 accggccccg tcgtcggctc cggacccggc tgttcgtcgc gatgttcgcg atcgcgttcg
     1201 gggtgctggt cgtcgccggg atcggcgcgg tcgggatcgc gcgccagacc gcgtcggcgg
     1261 ccgcgatcaa ggacctgaag cagaaggtga agccgatcgc gaccgagctc gacacgctcg
     1321 ggcgccagtt ccgcaccaac cggctccggg gcaacgccca gcaggcgaca cggacgtgtc
     1381 agctggtgac cgcggtgctg cggatctcga gcgcgagtgt cgtagccgtc aatgcggacg
     1441 ggaacgtcca agagggagtc gcgccgctgc tcggcagcgc gtgtgccaat caggccaacc
     1501 tggcgctccc ccgcgacgtc accgcgtcgg acctcaacgt cgaccaggtc ctggtcggcc
     1561 agcagcagtc gggggtgacc ggggctaccg cgttcgtcgt cgacccgttg acgccggtcg
     1621 ggacggtcac tccggcgttg gtgctcacga acggggtgga tacccgtccg ttggggcgcg
     1681 ccggcccgta tctgttcctg actggggcga tcgcgctgct ggtggccggg ttggtgtcgg
     1741 cgtacttggc gcggcggatg actcggccga tcgcggcgat gcgcgacacc gcgcagcgca
     1801 tcgcgggtgg tgacctctcg gcccgggtgg gggtgatggg tcagcccgat gacgagctct
     1861 ccagtctcgc ccagagcctc gacgcgatgg cgaccgagct cgagaccgcc cggggtcatg
     1921 aacgggtgtt cctgctcagc gtgtcgcacg acctgcgaac cccgctgacg tcgatccggg
     1981 ggtacgcgga ggcgatcacc gacgggatga tggcggaccc ggtcgaacag gcccgggccg
     2041 ggtcgatcat cgcggctgag gcgcgccgct tggagcgcct ggtcgccgac ttgctcgatc
     2101 tggcccgcct cgatgcgcac cagttctcgt tgcaccctcg gccggtcgac gctcggacgg
     2161 tggtgtatga cgcggtggcc gggttcgtgc cctcagctcg ggactggggg ttgcggctcg
     2221 aggtggtgcg cggcgacccg gccccgctcg acacggaccc ggaacggctg gcgcagatca
     2281 ccgcgaacct ggtcgagaac gcgttgaagt acgcacagag cacggtgcga gtcgacgtcg
     2341 accggcgtga cggtcaggtc gagctgcggg tcgacgacga cgggcccggc atcccggtcg
     2401 aggaccagaa ccgggtcttc cagaggctct ataccgcccg ggacgcgccg ggacgcaagg
     2461 tggggaccgg gatcgggttg gcgatcgtgc atgagctggc gggcgcgatg ggcggcgagg
     2521 cgagctgtga gccgcttgat gcggggggca cccggtttcg ggtcacgatc cccgcgtagc
     2581 ggcgcgtgct tggcgtcgcc ccgccgcgac gtaccaggcg gtgccgacgg cgagccatga
     2641 cgcgttgatc gtcaccgctt gggtgtcgtg tcgcagtacc cagccggcca cgatcgcgcc
     2701 tacggcgagc tcgtgcgcga cgaaccagcg ggtgcgctgc tgtcggacca ccaggatcca
     2761 gcgggcgagc accgcgatcg aggtcgggta gccgaagacg aacagggcgg tgctcaccgg
     2821 tcccccaccg tgacgagctc ggcgtcgacg tggccggtgt cccagcgcag ctcggccggg
     2881 gtgggcggct cgacctcgcg gcggaccatc gccaggccga cggcgcgggc ctcgccgggg
     2941 acggcggcga cgctggtgat ggtgccacgg tcggtgccgt caacgacgac ggtcgcgccg
     3001 cgtgggggta tcggtccgtc ggggatgcgg accccgcgga gataccggtt gacgtggccg
     3061 cgggtgtcga tgcgacagac cagctcttgg ccgacgaagc agcctttggt gaacgagacc
     3121 gcgtcgcgtt cgaggaacgc ctcttggggg atcgtgcggt cgtcgatgtc gacgccgagg
     3181 cgcggcaccc cggcttcgat gcggaacgcc tcgaaccgct cgggtgtcca tcgctcgacg
     3241 gtcgagtcga gggtcgaggt cgcggctcgc accgcgtcga ggggtccgag gaggtcgaca
     3301 ccggggtggc cggcccagtc ggcggggagg ggatggaccc cgccggggac ggtgtcgccg
     3361 gggacgccca tggtcgagac caggccggtg gtcgcggtgc ggtcgtcgag gtcgaccttc
     3421 actcggattc ggaaccggtt gagcgacgag agcaggcggg gcgcgtagtc ggcgtcggtg
     3481 tcgagccacc actcgtcgcc ggtccggaga atgcggaacg ccacgtcgag cttgccttgg
     3541 ggagtgagga gcagcgcggg ggcgtggtca ccgtcgccga gggggccgag gtcctgggag
     3601 acgaggcttt ggaggaagga ggtggcgtcg tcaccgcggg cgacgataag cccgcggggg
     3661 ctgcggtcgg cgacgaacgc ggggacggtc acgagagcgc gaccgaggtg agccggcgcg
     3721 ccgcggcggc ggcggtcagc aacgctcggg ctttgttggc acattcgagg tcctcgtcgg
     3781 cggggtcaga atcggcgacg atcccggcgc cggcttggac gctggcgtgc ccgtcggggc
     3841 cccagaccat ggtgcggatc gcgatcgcgg tgtcgaggtt gccggagaag tcgacgtagc
     3901 cgaccacacc ggcgtacggg ccccgcttga ccggctcgag ctcgtcgatg atctccattg
     3961 ctcggacttt cggcgcgccg ctcaccgtgc cggcaggaaa cgtggcgcgc agcacgtcga
     4021 cgggggtgcg accggcggcg agctggcccg acacctggct ggtcaggtgc atgacgtgcg
     4081 agtaccgctc cagggtcatg aactcgtcga cgtgctcggt accgaagtcc accacgcgcc
     4141 cgacgtcgtt gcgggctagg tcgacgagca tgacgtgctc ggcttgctcc ttcgggttct
     4201 cggacagctc cgcggccagg cgccggtcgt gttcgtcgct ttgaccgcgg tagcgggtgc
     4261 cggcgatagg ccggctgatg acgcgcccgt cgaggagctg gaccatcggc tcgggtgacg
     4321 agccgaccag ggtgacctcg gggtgacgga cgacgtacat gtacggcgag gggttcactt
     4381 ggcggagcac ccggtagacg tcgaacgggt cgaccgggtc gtcgaggtcg aaacgctgcg
     4441 cgaggacgac ttggaagatg tcgcctgcca ggatgtgctc gcgggcgacc tcgaccgcgt
     4501 cgtggtagcg gccaccgccg agggtggacc gcaccgcggg gatctcggtg agccggtcgg
     4561 ccggtgggcg cgacggcgag tacgggagcg gtcgggccag gtcgtcgacg agcgcgtcga
     4621 ggcgggcggc ggcacggtcg tacgcggcgt cgacgtcagg ttcggtgtcg aggaacacgt
     4681 tctcgatcaa gtagagccgc tggcgaaaat ggtcgaacgc ggtcacatgc cccgtgaccg
     4741 acagcaccgc gtcgggcaga ccctggtcgt cgggtggcac cgcggggagc cgttcgacct
     4801 cgcggaccac gtcgtagccg aggtagccga cgatcccgcc atgaaacggc ggcagggggt
     4861 cgaggtgcgg ggcccggtag gcgcccagca gcgcgtcgag cgccgcgagg gcaccctggt
     4921 cgagcggcac acccgggggg acggtcccgc cggtgacgtg gacctggcgt ccccggacga
     4981 cgagggtgag ggcgggatcg cggccgagga acgagaagcg gccccaccgt tgcccgtgct
     5041 cgaccgattc gaggaggaac ccgggggcgc cgtgaggttc ggcgccgacg agcttcacga
     5101 acgccgagac cggggtctcc aggtcggcga gcacctcgcg ccagaccggg acgacggagt
     5161 gcccgaccgc gagggcccgg aactcgtcgc gcgacggtcg gagaccgtcg tcgctggtca
     5221 cgagcggtcg ggcccgaagg accggaagaa gcagctgtat tcgccggtgt ggcaggcgcc
     5281 ccggccttct tgctcgacga cgaggagcag cgtgtcaccg tcacagtcgt agtaggcctc
     5341 acggagccac tgccggtcac ccgaggtgtc ccccttgcgc cagaactcct ggcggctgcg
     5401 gctccagaac acggtgcggc cttcgtcgag ggtgcggcgc aacgacgcct cgttcatcca
     5461 cgccagcatg aggacacggc cgctggcttg atcctggacg atcgcgggga cgagcccggt
     5521 ggcgtcccaa tggatctctc cgaggtcctc gtcccggatc gggatcgggg tcgcggtcgg
     5581 ggacatgggc gtcgagcgta cctctcgcct cgcgcggccc tgagccgcat tggcggacgc
     5641 gcgagactcc gcggccgatg agggggatgc tggagccacc gcgcgcgccg cgtcgcccga
     5701 cggagc
//
LOCUS       DATCCH010000376         2317 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_604303, whole genome shotgun sequence.
ACCESSION   DATCCH010000376 DATCCH010000000
VERSION     DATCCH010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604303"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VIJ44_07235"
     CDS             complement(<1..407)
                     /locus_tag="VIJ44_07235"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY5165725.1"
                     /translation="MVRWTITGLDHIPFDGPVLLASNHISFLDPMAILWLGDRRRRKV
                     RFMAKAELWKSRFCRFFLVRTGQIPVSRYSAAAGGSLDAAAKSLADGECVCIYPEGTI
                     SDDLEPMAAKVGIGRLAVERGAPILPVGVWGTHR"
     gene            628..1089
                     /locus_tag="VIJ44_07240"
     CDS             628..1089
                     /locus_tag="VIJ44_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165726.1"
                     /translation="MGLGATPRDGWRCGPRLGSTDAHGELTTELLGYVEGTDPKLLDD
                     LFAKPREHRIDNARARLADQPSLTAKVAELTRILDEDGYLATSQRVAPGLYLIIEHNC
                     AIWAVAQRYGQAGTSELEFIRAVLPEASGERTQHMAAGARRCAYEVREIAA"
     gene            1383..1967
                     /locus_tag="VIJ44_07245"
     CDS             1383..1967
                     /locus_tag="VIJ44_07245"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEY5165727.1"
                     /translation="MLSPVSRVTLDRPAAEALSKQWWVLLLAGIFSAVVGMVILSVQW
                     TLSDLATVVSIFFVVNGAFRAATPPVDNSGRGWNIGVGVAEILVGIAFLAWPGVSLLT
                     LAIFIGAWVFVHGVFDLSGGISSRHDVKFWWLFVIIGAIEVGLGILLLDRPVLSLALA
                     IAVAGVWALIIGTLEIIVAFEVKHLPDTLDRNAA"
     gene            complement(2051..>2317)
                     /locus_tag="VIJ44_07250"
     CDS             complement(2051..>2317)
                     /locus_tag="VIJ44_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165728.1"
                     /translation="GCPYLNTAAELRDAPEVVRHELVDFINEVGAYLEELARRAGLPA
                     PESAALELRLIAAGSFALMTVFGGDATPAMKARQAAAAIIEAAR"
BASE COUNT          367 a          814 c          771 g          365 t
ORIGIN      
        1 cggtgggtgc cccacacgcc gaccgggagg atgggggcgc cgcgctcgac agcgaggcgc
       61 ccgatcccga ccttcgcggc catcggttcg aggtcgtcgg agattgtccc ttcggggtag
      121 atgcacacgc actccccgtc agccaacgac ttcgccgccg cgtcgaggga gccccctgcc
      181 gcggcggagt agcgcgacac cgggatctgg ccggttcgca ccagaaagaa ccgacagaag
      241 cgcgacttcc agagctcggc tttggccatg aatcggacct tgcgacgacg gcggtcgccg
      301 agccacagga tcgccatcgg gtcgaggaac gagatgtggt tactggccag cagcaccgga
      361 ccgtcgaaag ggatgtggtc gagaccggtg atcgtccagc ggaccatccg gcaccacggg
      421 tacacgatgc cgcgtcccag gacccagacc ggatccctcg agcccgatct tctcggggag
      481 ctcaacgcga accgccgacc gcgccgcgct ccgatggctc gccgcgccgg aggtgggttg
      541 gaaacgtcga tcgccggacc tctcctgtgc ctcatcaacc aacgtgtgtg cggttggcgc
      601 gtcgcgtggg ttgatcctcg caggttggtg ggtctgggag cgactccccg cgatgggtgg
      661 cggtgcggcc cgaggctggg cagcaccgat gctcatggcg agctgaccac cgagctcctc
      721 ggctatgtcg aaggcacgga cccgaagctg ttggacgacc tcttcgccaa accacgggag
      781 caccggatcg acaacgcccg cgcccgcctg gctgaccagc caagcctcac ggccaaggtc
      841 gccgagctca cccgaatcct cgacgaggac ggctacctcg ccacctcgca gcgggtcgca
      901 cccgggctgt atctgatcat cgagcacaac tgcgccatct gggctgtggc gcagcgctac
      961 gggcaagcgg gcacgagcga gctggagttc atccgggccg tcctgcccga ggccagcggg
     1021 gaacgaaccc agcacatggc cgccggcgcc cgccgctgcg cctacgaggt gcgcgaaatc
     1081 gcggcctgag ccaccgctca tcgacaccgc gctggaccca gcgccccaaa gcttgccggc
     1141 aaccccggcg acggcggccg ccgttccgct ggtcaggctg gacgagcccg gtcgccaacg
     1201 actacgccgt gagccaaacc ccgcgatccg gaggactcgg tgacggatct tcgaccctca
     1261 ttcagcgtcg tgatccccgc ccgagtccgg tcggatcgcg accgtcggtc gatacggtgc
     1321 gcgtccagac ggcgcgctga cccaacacgg ctcgtctcaa tggcggcccg gaaggaacac
     1381 cgatgctgag ccccgtctct cgtgtcacgt tggatcgccc cgcggcggag gcgctcagca
     1441 agcagtggtg ggtgctgctg ctggccggga tcttcagcgc ggtcgtcgga atggtcatct
     1501 tgtcggtcca gtggacgctc tctgatctcg ccacggtcgt gtcgatcttc ttcgtcgtca
     1561 acggggcgtt ccgtgccgcg accccaccgg tcgacaactc cgggcgcggc tggaacatcg
     1621 gcgtcggggt ggcggagatc ctcgtcggga tcgcgttctt ggcctggccc ggtgtgagcc
     1681 tcctcacgtt ggcgatcttc atcggagcct gggtcttcgt ccacggcgtc ttcgatctct
     1741 ccggtggcat ctcgagccga cacgacgtca agttctggtg gctcttcgtg atcatcggcg
     1801 ccatcgaggt cggtctcggg atcctgctcc tggatcgccc cgttctctcc ctcgcgctgg
     1861 cgatcgccgt ggccggcgtc tgggccctca tcatcgggac cctcgagatc atcgtcgcct
     1921 tcgaggtcaa gcacctgccg gacaccctcg accggaacgc cgcctgaacc cgacatcccg
     1981 accacccacc aggtccggac cagccgaacg ggatcgcggc gcggcgactc gagctcggcc
     2041 gcggagtccg ctaccgagcc gcttcgatga tggcggcggc agcctgtcgc gccttcatgg
     2101 ccggcgtcgc gtcaccgcca aagacggtca tgagggcgaa tgatcccgcg gcgatgaggc
     2161 gcaactcgag ggccgcgctc tccggagcag gcaaccccgc ccggcgagcg agctcctcga
     2221 ggtacgcccc gacctcattg atgaaatcga ccagttcgtg gcggacaacc tcgggggcgt
     2281 cgcggagctc ggcggcggtg ttgagatacg ggcagcc
//
LOCUS       DATCCH010000377         5128 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_145966, whole genome shotgun sequence.
ACCESSION   DATCCH010000377 DATCCH010000000
VERSION     DATCCH010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5128
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145966"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1189
                     /locus_tag="VIJ44_07255"
     CDS             <1..1189
                     /locus_tag="VIJ44_07255"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY5165729.1"
                     /translation="EVARAEAERTVEAARAALAAARATELRRAAADERRALLASRLAE
                     VETRLAERDPEAQASAERHRLELLGRDEALATITRRLAEHRAGVDTLSDRLRDRRRRE
                     TEAARLSAEQLDTLRDERRGLEHELTGIRERLGRIDVEDAEARLRLDTTIEGLRTEFD
                     VEPHVALDAPAPTVAEGTTLAGRARELERDLRLMGPINPLALDEHRALQERHEFLQSQ
                     LDDVRGSRRELSRVLRAVDKEIVSVFEEAFADVTRHFSTLFSTLFPGGAGRLSLLDPD
                     DLLDTGIEIEARPSGKNVRRLSLLSGGERSLTALAYLFAVFRARPSPFYLLDEVEAAL
                     DDVNLHRFLDLVHEFRNEAQLVIVTHQKRTMEAGDVLYGVTMPPGGSTRVVSQRVADS
                     VGA"
     gene            1198..2247
                     /locus_tag="VIJ44_07260"
     CDS             1198..2247
                     /locus_tag="VIJ44_07260"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEY5165730.1"
                     /translation="MSTAALALPARLLSAGGSAADLQRACGPAGSRTWLCETVFRITG
                     NDGAADVADALARPLRIALVLVLAFLAARFLRRVIRHLVQRLKVGPELGSAAEARRNQ
                     RADTVATVLNSVSTIVIWSVAVITVVGELGLNLAPLLAGAGVAGVALGFGAQTVVRDF
                     LAGTFMLLEDQYGVGDIIDVGVPTGLGGTAVSGTVESVTLRVTRLRDVEGVVWHVPNG
                     EIRRVGNKGQQWSRTLLDVAVAPETSIPHARDVIGHVARDLWQDEDWHRRIVAEPEVW
                     GVEDLTGQHIVIRLVVKTVPIEQWNVARELRARLKEAFDTAGIAMAVPRQVVSYLDGP
                     EPGTQSDESAANDDG"
     gene            2261..3352
                     /gene="ftsY"
                     /locus_tag="VIJ44_07265"
     CDS             2261..3352
                     /gene="ftsY"
                     /locus_tag="VIJ44_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014138252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEY5165731.1"
                     /translation="MSSATVLLVYVIIFAFAALLIALPRVLGRRERRHRAEHPETSPS
                     TGPASVASTGPDRAALPSDAAPGVLGERLGRSRAPFRGLRDRWRSRRLDDETWDDLEE
                     TLLLADVGVPTTGRLLDSLRTEARRAGIRDADQLAPLLEAQIADELGDHTDRTLRHDV
                     GHPNVWLLVGVNGVGKTTTAAKLAQREVDAGHRVLLVAADTFRAAAAEQLAHWGEQVG
                     VDVTRGQAGGDPASVVFDAVQSAQARGIDTVVVDTAGRLHTKVNLMEELKKIRRIVER
                     FPDTLGEVLLVLDASTGQNGLAQARQFTDDIGVTGVVLTKLDGTAKGGIVLAVEAELG
                     VPVKLIGIGENATDLVAFAPRDFAAALIG"
     gene            3352..4671
                     /gene="ffh"
                     /locus_tag="VIJ44_07270"
     CDS             3352..4671
                     /gene="ffh"
                     /locus_tag="VIJ44_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEY5165732.1"
                     /translation="MFETLADRFDAIFRRLRSRGRLNEADVEEVAREIRLALLEADVN
                     VRVVKSFVNRLKARATGAEVAESLSPAQQVIKIVHEELAVTLGGAAGTLHPAKRPPAV
                     VVLVGLQGSGKTTAAAKLARHLEHQGRPALLVAADLQRPAAIEQLQVLGERAGVPVWA
                     SGTDPVTAATDGVREAERLGRPVVIVDTAGRLQVDADLMAELANLVDAIHPSDVLLTV
                     DAMIGQDAVRVATEFAAQVPLTGVVLTKLDGDARGGAALSVKEVTGLPILFAGVGEQI
                     DDFEPFYPDRLASRILGMGDVLTLIEKAEAAFDADQAQAAGEKLRSGRFTLEDFLDQM
                     RQMKKMGPLQSIVGMLPGVPKELRDAELDEGELARVEAIICSMTLAERRDPSLINGSR
                     RHRIAEGSGTSTQQVNALLKQFKVVQQMMRTMSKGPKGRRRLPARGR"
     gene            4799..>5128
                     /gene="rpsP"
                     /locus_tag="VIJ44_07275"
     CDS             4799..>5128
                     /gene="rpsP"
                     /locus_tag="VIJ44_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEY5165733.1"
                     /translation="MAVKIRLLRVGKAKQPSYRVVVADSRSPRDGRIIETIGHYGPRA
                     EPSTVDINADRALVWLRQGAQPTEQVQKLLTSLGIWSTFESEKGHPIVTKLNRRGFKT
                     GKVEAPTK"
BASE COUNT          793 a         2008 c         1641 g          686 t
ORIGIN      
        1 cgaggtggcg cgcgccgaag cggaacgcac cgtcgaggcc gcccgcgccg cgctcgccgc
       61 ggcacgggcc acggagctgc gccgcgccgc cgccgacgag cgccgcgcgc tcctcgcctc
      121 ccgcctggcc gaggtcgaga cccgcctcgc cgaacgggac cccgaggccc aggccagcgc
      181 cgaacggcac cgcctcgagc tgctcggacg cgacgaagcc ctcgccacca tcacccgccg
      241 actcgccgag caccgcgccg gagtcgacac cctctccgac cggctgcggg accggcgccg
      301 ccgtgagacc gaggctgccc gcctgtcggc ggagcagctc gacacgctgc gcgacgagcg
      361 gcgcgggctc gagcacgagc tcaccggcat ccgggaacgc ctgggccgca tcgacgtcga
      421 agacgccgaa gcgcggctgc gcctcgacac caccatcgaa ggtctccgca ccgagttcga
      481 cgtcgagccc cacgtcgcgc tcgacgcgcc tgccccgacc gtggccgagg ggaccacgct
      541 cgccggccgg gcccgcgagc tcgaacgcga cctgcgcctc atgggtccga tcaacccgtt
      601 ggccctcgac gagcaccggg ccctccaaga acgccacgag ttcctccagt cccagctcga
      661 cgatgtgcgg ggcagccgcc gagagctgag ccgggtgctg cgcgccgtcg acaaggagat
      721 cgtcagcgtg ttcgaggagg cgttcgcgga cgtcacccga cacttctcca ccctgttctc
      781 gacgttgttt cccggcggcg ccggccggct gtcgctcctc gaccccgacg acctgctcga
      841 caccgggatc gagatcgaag ctcgcccatc aggcaagaac gtccggcggc tctcgctgct
      901 ctccggcggt gagcggtccc tcaccgcgct cgcgtacctg ttcgcggtgt tccgggcccg
      961 cccgtcgccg ttctatctcc tcgacgaggt cgaagccgcg ctcgacgacg tcaacctgca
     1021 ccgcttcctc gacctcgtcc acgagttccg caacgaggcg cagctcgtca tcgtcacgca
     1081 ccagaagcgc acgatggaag ccggcgacgt gctgtacggg gtcacgatgc caccgggcgg
     1141 gtcgacccgg gtcgtgagcc agcgggtcgc cgactccgtc ggcgcctgaa ccgcgtcgtg
     1201 tcgaccgcgg cgctggcgtt gccggcgagg ctgctcagcg ccgggggaag cgccgccgac
     1261 ctgcaacggg cctgcggtcc tgccgggagc cgcacctggc tctgcgagac cgtctttcgg
     1321 atcaccggca acgacggcgc cgccgacgtg gccgacgccc tggcccggcc gctgcggatc
     1381 gcgttggtgc tcgtcttggc attcctcgcg gcccgcttcc tgcgccgcgt gatccgccat
     1441 ctcgtgcagc ggctcaaggt cgggcccgag ctcggctccg ccgccgaggc gcgccgcaac
     1501 cagcgggccg acaccgtcgc caccgtgctc aacagcgtct cgacgatcgt catctggagc
     1561 gtcgcggtca tcaccgtcgt cggcgagctc ggactcaacc tggccccgct cctcgccggc
     1621 gccggcgtcg ccggcgtcgc gctcggcttc ggcgcccaga ccgtcgtgcg cgacttcctg
     1681 gccggcacct tcatgctcct cgaggaccag tacggggtcg gcgacatcat cgacgtcggc
     1741 gtgcccaccg gactcggcgg gaccgccgtg tcgggaaccg tcgaatcggt caccttgcga
     1801 gtcacgcgcc tccgcgacgt cgaaggagtc gtctggcacg tccccaacgg cgagatccgc
     1861 cgcgtcggca acaagggcca gcagtggtcg cggacgctgc tcgacgtcgc cgtcgcaccc
     1921 gagacctcga tcccacacgc ccgcgacgtc atcggacacg tggcgcgcga cttgtggcag
     1981 gacgaggact ggcaccggcg aatcgtcgcc gaacctgagg tctggggtgt cgaagacctc
     2041 accgggcaac acatcgtgat ccgcctcgtc gtcaagacgg tgccgatcga acaatggaac
     2101 gtcgcgcgcg agttgcgcgc ccgcttgaag gaagcgttcg atacggccgg catcgcgatg
     2161 gcggtccccc gacaagtcgt cagctacctc gacggacccg aaccggggac ccagtctgac
     2221 gaaagcgcgg ccaacgacga cgggtagcat cgccgcgctg atgtccagcg cgaccgttct
     2281 gctcgtctac gtgatcatct tcgctttcgc cgccttgctc atcgcgctgc cgcgtgtcct
     2341 tggccgacgc gaacgccgcc accgagccga gcaccccgag acgagccctt caaccgggcc
     2401 cgcgagcgtg gcttccaccg gccccgaccg agctgcgctc ccctccgacg ccgccccggg
     2461 ggtgctcggc gagcgcctcg gacgcagccg cgcccccttc cgcggtctcc gcgatcggtg
     2521 gcggtcccgt cgtctcgacg acgagacctg ggacgacctc gaagagaccc tcctcctcgc
     2581 cgacgtcggg gtgcccacca ccggccgact gctcgattcg cttcgcaccg aggcacggcg
     2641 cgccgggatc cgcgacgccg accagctcgc gccgctgctc gaggcccaga tcgccgacga
     2701 gctcggcgac cacaccgacc gcacgttgcg tcacgacgtc ggtcacccca acgtgtggtt
     2761 gctcgtcggc gtgaacggcg tcgggaagac caccaccgcg gcgaagctcg cccaacgcga
     2821 ggtcgatgcc gggcaccggg tccttctcgt cgccgcggac acgttccggg ccgccgcggc
     2881 cgagcagctc gcccactggg gcgaacaagt cggtgttgac gtcacccgcg gtcaagccgg
     2941 tggggacccg gccagcgtcg tcttcgacgc ggtgcaatcc gcgcaggccc gcggcatcga
     3001 caccgtcgtc gtcgacaccg ccggtcggct ccacaccaag gtcaacctca tggaggagct
     3061 caagaagatt cgccggatcg tggaacgctt ccccgacacc ctcggcgagg tgctcctcgt
     3121 cctcgacgcg tcgaccggtc agaacgggct cgcgcaggcc cgccagttca ccgacgacat
     3181 cggcgtcacc ggcgtcgtcc tcaccaagct cgacggcacc gccaagggcg gcatcgtgct
     3241 cgccgtcgaa gccgagctcg gggtcccggt caagctgatc gggatcggcg agaacgccac
     3301 cgacctcgtc gccttcgcac cgcgcgactt cgccgccgcg ctcatcggct gatgttcgaa
     3361 accctcgccg accgcttcga cgccatcttc cgtcgtctgc gaagccgcgg ccggctcaac
     3421 gaagccgacg tcgaggaagt cgcgcgtgag atccgcctcg cgctcctcga ggccgacgtc
     3481 aacgtccggg ttgtcaagtc gttcgtcaac cggctcaaag cccgcgccac cggcgccgag
     3541 gtcgccgaga gcctcagccc ggcccagcag gtcatcaaga tcgtccacga agagctcgcc
     3601 gtcaccctcg gcggcgccgc cggcaccctg cacccggcca agcgcccacc ggcggtggtc
     3661 gtgctcgtcg gcctccaagg atcgggcaag accaccgccg cggccaagct cgcccgacac
     3721 ctcgagcacc agggccgtcc cgcgctcctc gtcgccgccg acctgcagcg ccccgccgcg
     3781 atcgagcaac tccaggtgct cggcgaacgc gccggcgtcc cggtctgggc cagcggcacc
     3841 gaccccgtga ccgccgcgac cgacggggtg cgcgaagccg aacggctcgg ccgccccgtc
     3901 gtcatcgtcg acaccgcggg ccgactccag gtcgacgccg acctcatggc cgagctcgca
     3961 aacctcgtcg acgccatcca ccccagcgac gtcctgctca ccgtcgacgc gatgatcggc
     4021 caagacgcgg ttcgcgtcgc caccgagttc gcggcccaag tcccccttac cggcgtcgtg
     4081 ctcaccaagc tcgacggcga cgctcgcggc ggcgcggccc tctcggtcaa agaggtcacc
     4141 gggctcccga tcctcttcgc cggggtcggc gaacagatcg acgacttcga gcccttctac
     4201 cccgaccggc tcgccagccg catcctcggg atgggtgacg tcctcaccct catcgagaaa
     4261 gccgaagccg cgttcgacgc cgaccaggct caagccgccg gggagaagct gcgctcggga
     4321 cggttcacgc tcgaagactt cctcgaccag atgcgccaga tgaagaagat gggtcccctc
     4381 caaagcatcg tcgggatgct ccccggcgtc ccgaaggagc tccgcgacgc cgagctcgac
     4441 gagggcgagc tcgcccgagt cgaagcgatc atctgctcca tgaccctcgc cgagcgccgg
     4501 gacccctcgc tcatcaacgg ctctcgccgt caccggatcg cggagggcag cggcacctca
     4561 acccagcagg tcaacgcgct gctcaaacag ttcaaggtcg tgcagcagat gatgcgcacc
     4621 atgagcaagg gccccaaggg tcgccgccgc ctcccggccc gaggtcgcta gcgacgcgaa
     4681 cgcggccggc gggcaccgac ccggcccgct caccgccacc caccatccac cgccgctcgt
     4741 ggcgtggccc cccggttcgc taccctcgcg agttcccgag acgaaagggc ccgactccgt
     4801 ggccgtcaag atccgcctgc tgcgcgtcgg caaggccaag cagcccagct accgagtggt
     4861 cgtggcggac agtcgctccc ctcgagacgg ccgcatcatc gagaccatcg gccactacgg
     4921 cccccgcgcc gagccgtcga cggtcgacat caacgccgac cgggctctgg tttggctccg
     4981 ccagggtgcc cagcccaccg agcaggtcca gaagctgctg accagcctgg ggatctggtc
     5041 gacgttcgag tccgagaagg gccacccgat cgtcaccaag ctgaaccggc gcggcttcaa
     5101 gaccggcaag gtcgaagccc ccaccaag
//
LOCUS       DATCCH010000378         2507 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_604472, whole genome shotgun sequence.
ACCESSION   DATCCH010000378 DATCCH010000000
VERSION     DATCCH010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604472"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="VIJ44_07280"
     CDS             complement(<1..475)
                     /locus_tag="VIJ44_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009742596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEY5165734.1"
                     /translation="MPTREAVIVDTVRTPIGKRNGMLAGWHPTDLLGHTLRALLGRHD
                     VDPGLVDDVVGGCVTQAGEQGCNVTRNAWVAAGLPWHVPATSVDRQCGSSQQAMHFVA
                     QGVIAGAYDLAIACGVESMTRSPMSSNARGGSGPFSAAFLEACNGVLGIQFWVAQV"
     gene            525..2321
                     /locus_tag="VIJ44_07285"
     CDS             525..2321
                     /locus_tag="VIJ44_07285"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY5165735.1"
                     /translation="MRIAALVKQIPAFEEMELGPDGRLRRDGLDLEMNPYCRRAVSQA
                     CELAAAVGDGTVTVLTLGPPAAEDTLREAIAWATEQDVTSEGVLITDPAFAGSDTLAT
                     ARALAAAIEREGGFDLVLTGRNSVDADTGQVGPELAELLGLPFATGARYLSLQGDTLH
                     VRCEHDDGFAQVEVSLPAVVSCAERLIDPCKVDPLGRAAVPAELLRTVSAADLGDGPW
                     GEAASPTRVGTVRLHAVERDRTVLDGPVDRQALRAVDLLVERGALGPNASDEQSETVP
                     PLQGERGDAVAVVVEPDRAQVTRELLGAAAQLAVGIDGHVVALCVEAPEPAMLASWGA
                     DAAVHLEGSTVEEDVAGAVADWVRVAAPWAVLATSTAWGREVAARAAAHLGAGLTGDA
                     VDLEVGDDGRLVAWKPAFGGQLVAAITATSLVQLATVRAGVLPLLVSRSATTIETRPM
                     AVARRSRVRVRNRTRDDDLDTLAEAATVVGVGVGVDPADYPALAPLLDTLGAELAATR
                     KVTDKGWQPRARQVGLTGRTIAPKLYVAVGLSGKFNHLVGVRAAGTILAVNVSPDALV
                     FGACDIGIVGDWHEVVPALVNELVGAGVTSGS"
     gene            complement(2326..>2507)
                     /locus_tag="VIJ44_07290"
     CDS             complement(2326..>2507)
                     /locus_tag="VIJ44_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY5165736.1"
                     /translation="VLSVALGALGPGDWSLDHAFGFSFPFDNGTALLISALVAIGGTA
                     VFLAAFWRPARDAAT"
BASE COUNT          344 a          891 c          920 g          352 t
ORIGIN      
        1 gcacctgagc cacccagaac tggatcccga gaaccccgtt gcaggcctcg aggaacgcgg
       61 cgctgaacgg gccactgcct ccccgagcgt tgctcgacat ggggctccgt gtcatcgatt
      121 cgacgccgca ggcgatcgcc aggtcgtacg caccggcgat gacgccctgg gcgacgaaat
      181 gcatcgcctg ctgcgacgat ccgcactgcc gatcgacgct cgtcgccggc acatgccacg
      241 gcagccccgc cgcgacccag gcgttgcgag tgacgttgca tccctgctca ccggcctggg
      301 tgacgcaacc gccgacgacg tcgtcgacaa gccctgggtc gacgtcgtgt cgtccgagca
      361 gcgcccgcag ggtgtggccg agcaggtcgg tcgggtgcca accggcgagc atgccgttgc
      421 gcttcccgat cggcgtgcgg acggtgtcga cgatgacggc ttcgcgggtg ggcattggcc
      481 tctcattccg attggagcgc tcgacagcgt acgctgcgcc gaccatgcga atcgccgccc
      541 tggtgaagca gatcccggcc ttcgaggaaa tggaactcgg gcccgatggt cggctgcgcc
      601 gcgacggact cgacctcgag atgaatccgt actgtcgacg cgcggtgtcc caggcgtgcg
      661 agctcgccgc cgccgtcggg gacggaacgg tcaccgtctt gaccctcggg ccgcccgcgg
      721 cggaggacac cttgcgcgaa gcgatcgcgt gggcgacgga gcaagacgtc acttcggaag
      781 gtgtgctcat caccgacccc gcgttcgcgg gctccgacac gctcgccaca gcgcgggccc
      841 tcgccgcggc gatcgaacgc gagggtggct tcgatctcgt cttgaccgga cggaactcgg
      901 tcgatgccga taccggccaa gtcgggcccg agctggccga gctgctcgga ttgcccttcg
      961 cgaccggcgc ccgctacctc agcctccagg gcgacaccct ccacgtccgt tgcgagcacg
     1021 acgacggctt cgcgcaggtc gaggtcagtc ttcccgcggt ggtgtcatgc gcggagcgcc
     1081 tcatcgatcc gtgcaaggtc gacccgctcg ggcgagcggc ggtgcccgcc gagctgctcc
     1141 gcacggtgag cgcggcggac ctcggcgacg ggccgtgggg tgaggcggcg agcccgaccc
     1201 gggtcgggac ggtgcggctc catgccgttg agcgcgaccg gacggtgctc gacggcccgg
     1261 tggaccgcca ggccttgcgc gcggtcgacc tgctggtcga gcggggcgcc ctcgggccca
     1321 acgccagcga cgaacagtcc gagacggtgc cgccgctcca aggtgagcgc ggtgacgcgg
     1381 tggccgtcgt cgtcgagccc gatcgggcgc aggtcacccg ggagctgctc ggtgccgccg
     1441 cgcagctcgc ggtcgggatc gacgggcatg tggtcgcgct gtgcgtcgag gcgcccgagc
     1501 cggcgatgtt ggcctcctgg ggtgcggacg ccgcggtgca cctggaggga tcgacggtcg
     1561 aggaggacgt cgccggcgca gtcgccgact gggtccgggt ggcggccccg tgggcggtcc
     1621 tggccacgag taccgcctgg ggacgcgagg tcgcggcgcg cgccgccgcc cacctcggcg
     1681 ccgggctgac tggggacgcg gtcgatctcg aggtcggaga cgacggtcga ctggttgcct
     1741 ggaagcccgc gttcggcggt cagctcgtcg ccgcgatcac cgccacctcg ctggtgcagc
     1801 tcgcgaccgt gcgcgccggc gtcctcccgc tgctcgtctc ccgctcggcc acgacgatcg
     1861 agactcggcc gatggcggtc gcccgccggt cacgggtccg ggtgcggaac cgcacccgtg
     1921 atgacgacct cgacacgctc gccgaggccg ccacggtggt cggcgtcggc gtcggggtcg
     1981 accccgccga ctatcccgcg ctcgcgccac tgctcgacac cctgggcgcc gagctcgccg
     2041 cgacgcgcaa ggtgaccgac aagggctggc agcctcgggc ccgacaggtg ggactgaccg
     2101 ggcgcaccat cgcgcccaag ctctacgtgg cggtcggcct cagcggcaag ttcaaccatc
     2161 tggtgggtgt ccgggcggcc gggacgatcc tcgccgtcaa cgtctcgccg gacgcgctgg
     2221 tcttcggcgc gtgcgacatc gggatcgtcg gggactggca cgaggtggtc cccgccctcg
     2281 tcaacgagct tgtcggcgcg ggcgtgacgt cgggttcgta gtcgatcagg tcgcagcgtc
     2341 gcgggctggc cgccagaacg cggcgaggaa caccgcggtc ccgccgatgg cgaccagggc
     2401 gctgatcagc aacgcggtcc cgttgtcgaa cgggaacgag aacccaaacg cgtggtcgag
     2461 cgaccagtcc ccggggccca gcgcgccgag cgcgacgctc agcaccg
//
LOCUS       DATCCH010000379         2205 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_230184, whole genome shotgun sequence.
ACCESSION   DATCCH010000379 DATCCH010000000
VERSION     DATCCH010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230184"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="VIJ44_07295"
     CDS             <1..578
                     /locus_tag="VIJ44_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY5165737.1"
                     /translation="SILSEAEVDGERLTQLEIDLFFMLLTVAGNETTRNQVSHGLLAL
                     LEHPDQLERLRANRELLPGAVEEMLRWASPVMHFRRTATEDLTLGGQAIAAGDKIVIW
                     YASANRDEKVFDDPMRFDIERSPNEHVAFGGGGPHFCLGANLARMEIRVLFEELLDRW
                     SHLELAGEPTWLRSNFINGIKHIPLRFTAAS"
     gene            complement(650..1480)
                     /locus_tag="VIJ44_07300"
     CDS             complement(650..1480)
                     /locus_tag="VIJ44_07300"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5165738.1"
                     /translation="MDLNGVNAVVTGGASGIGRATAIELARRGAHAVAIADINDTRLD
                     ETVVELETLGTRPFPVHCDVSKDADVERLRDETLAAFGHVDVVMNNAGVALLGPPDTL
                     SMAEWDWILQINLYGVIRGIRAFVPHLRHRGQGWVVNTASVAGLYAYAWDTIPYITAK
                     FGVVGLTESLALYLRPLGVGVSLLCPGLVDSNMADTARIGGVADPSAWIKEMPLEAPV
                     QPTVPGRLVCDAIEREQFLVLTHPEPIRERIAQRGADLDAFVAAQIERLPTPPNLPPR
                     "
     gene            complement(1480..>2205)
                     /locus_tag="VIJ44_07305"
     CDS             complement(1480..>2205)
                     /locus_tag="VIJ44_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020556472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5165739.1"
                     /translation="LERLETAADRLRSVAAPGATVQTVVADVTDEDQIRAAVAAAALP
                     TGRLHGVVASAGGSLHLGPLVLADVDAVRATLDLNVIGTFLTLKHAAPLLAASGAGSF
                     VGVSSHAGLDTFRFLGVYGAAKAGLDMVVRVAADELGPSRVRVNSVRPGIIDNELMAA
                     ITAGGPVLDSYLENIPLHRVGTVDDVGALARFLVGPESSWITGQCISVDGGQSLRKGA
                     DFSAFAEPGHRDQPGWQLMVEHD"
BASE COUNT          367 a          748 c          774 g          316 t
ORIGIN      
        1 tcagcatcct gagcgaggcc gaggtcgacg gggagcggct cacccagctc gagatcgacc
       61 tgttcttcat gctcctgacc gtcgccggca acgagaccac ccgaaatcag gtttcgcacg
      121 gcctactggc cctgctcgag caccccgacc agctggagcg gctccgcgcc aaccgtgaac
      181 tgctgccggg cgcggtcgag gagatgctcc gctgggcgag cccggtgatg cacttccgtc
      241 gcaccgccac cgaggacctg accctcggcg gtcaggcaat cgccgccggg gacaagatcg
      301 tgatctggta tgcgtcggcg aaccgcgacg agaaggtgtt cgacgacccg atgcggttcg
      361 acatcgaacg ctccccaaac gagcacgtgg cgttcggagg tggcggcccg cacttctgcc
      421 tcggcgccaa cctggctcgg atggagatcc gagtgttgtt cgaggagctc ctcgaccgct
      481 ggtcgcacct cgagctcgcc ggtgagccca cctggctccg gtcgaacttc atcaacggca
      541 tcaagcacat cccgctgcgg ttcactgccg ccagctgagc gacacgtccg cgcccggctc
      601 gcgtggagtg cgcgggtggg accgcgaggc gcggcgagcg ccgatcaggt cagcgaggcg
      661 gcaggttcgg tggtgtcggg agccgctcaa tctgggctgc gacgaacgcg tcgaggtccg
      721 ctcctcgctg cgcgatccgt tcccgaatgg gctcggggtg ggtgagaacg aggaactgtt
      781 cccgttcgat cgcgtcgcag accaggcggc ccgggacggt gggctgcacc ggggcctcga
      841 ggggcatctc cttgatccac gccgacgggt ccgcgacgcc gccgatgcgg gcggtatcag
      901 ccatgttcga gtcaacgagt cccggacaga gcagcgagac gccaaccccc aacggccgga
      961 ggtacaacgc caggctctcg gtgagcccca cgaccccgaa cttggcggtg atgtagggga
     1021 tcgtgtccca ggcgtaggcg tacaagcccg cgacggacgc ggtgttcacc acccacccct
     1081 ggccccggtg acggagatgg gggacgaacg cccggatacc gcggatcacg ccgtacaggt
     1141 tgatctgcaa gatccagtcc cattcggcca tcgagagggt gtcgggcggt ccgaggagcg
     1201 cgacgccggc gttgttcatg acgacgtcga catgcccgaa cgcggcgagg gtctcgtcgc
     1261 ggagccgctc gacgtcagcg tccttcgaga cgtcacagtg gaccggaaaa ggtcgcgtgc
     1321 cgagcgtctc gagctcgacg acggtctcat cgagccgagt gtcgttgatg tcggcgatcg
     1381 cgacggcgtg ggcgccgcga cgcgccagct cgatggcggt ggcccgtccg atgccgctgg
     1441 ccccaccggt gacgacggcg ttcacgccgt tcaggtccat cagtcgtgct ccaccatgag
     1501 ctgccaccca ggctgatccc gatggcccgg ctcggcgaac gccgagaagt ccgcaccctt
     1561 tcggagcgac tggccgccgt cgacgctgat gcactggccc gtgatccagc tcgactcggg
     1621 gccgacgagg aaccgggcga gagcgccgac atcgtcgacg gtgccgaccc ggtggagtgg
     1681 gatgttctcg aggtagctgt cgaggaccgg gccgccggcg gtgatcgccg ccatgagctc
     1741 gttgtcgatg atcccggggc gcaccgagtt gacccgaacc cgggacggac cgagctcgtc
     1801 cgcagcgacg cgaacaacca tgtcgagacc ggctttcgcg gccccgtaga cgccgaggaa
     1861 acggaacgtg tcgagcccgg cgtgggagga gaccccgacg aacgaacccg caccgctcgc
     1921 ggcgagcagc ggcgccgcat gcttcagggt caagaaggtt ccgatcacgt tgaggtcgag
     1981 cgtggcacgc accgcgtcga cgtcggcgag gaccagcggg ccgaggtgga gcgatcctcc
     2041 cgccgaggcg acgaccccgt gaagccggcc ggtcggcagc gcggccgcgg cgaccgcagc
     2101 ccggatctga tcctcgtcgg tgacgtcggc gaccacggtc tgcaccgtcg cgcccggagc
     2161 ggcaaccgac cggagccgat ccgcggccgt ctcgagacgc tcaag
//
LOCUS       DATCCH010000380         8909 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_437978, whole genome shotgun sequence.
ACCESSION   DATCCH010000380 DATCCH010000000
VERSION     DATCCH010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 8909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8909
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437978"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            234..2558
                     /locus_tag="VIJ44_07310"
     CDS             234..2558
                     /locus_tag="VIJ44_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018505813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEY5165740.1"
                     /translation="MSGPAAGRFVGQSVPRREDPRLLTGHGQFVDDVVVPGMLHAAFV
                     RSDLARGRITRLDVDAARAMPGVRAVLTGADLNGGLGSMQPTMFQSDPPPSPCVPLRP
                     LAEHDVRFVGDPIALVIAENRYLAEDACELIEVDLEALPPVVDPERAVEETDELVHPE
                     LGANVAQRVESPADPELDAIFDTAAHVVTETIVQHRHTNVPMETRGVVAGHQPATGEL
                     DVWISTQNPHEVRQVCSRMLRVPEHLVRVRMGDVGGGFGQKYFAPRDELAVIAAAHRL
                     GCTVKWIEDRRENLIASNHARADRMTVRMALDDTGHILGVHLDHLEDCGAYPVGGTGG
                     MGPFVAILFPGPYRIPKLGFRSTSVWTNTCGRGAYRGPWMMETVAREEMLDLAARAIG
                     LDPLELRRRNCIGPDELPFTTAMGMVLDHVTPSATLEQAVELIGYDEFRAEQRRAAAD
                     GRLLGIGIGLYVEPTSIASGNIGVETATIRVQPSGTVTVHLGTGSHGQSVETTMAQVV
                     AEHLGVDYDDVLVVQGDTASAPFGGGTGGSRTAVVAGGAAREASLQVREKAVQIAAHL
                     LEAAPEDLESGDGRLSVRGTPTRGVSLAEVARVALNAPLELPPDIAPGLEATVSYQAP
                     PITWSNACHVCTVEVDPATGKVELLRYVVSEDCGVMINPMVVEGQIAGGVVQGIGGVL
                     YEHLIYDEDGNPLTTTFLDYLVPTAAEVPAIEYGHVETRSPTPGGHKGMGEGGAIGSP
                     PCVFNAVADALAARGAVITEQPLTPSRILDALSR"
     gene            complement(2599..3711)
                     /locus_tag="VIJ44_07315"
     CDS             complement(2599..3711)
                     /locus_tag="VIJ44_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003943541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165741.1"
                     /translation="MTSPLSPLDDYPVHQTAEVIRHPATSDRNFYDRYYFMLHGSDDD
                     LMLIMGMGQYPNLSVQDAFALVRRGSRHRVVRASRALGVDRMNLSVGPFRLEVLEGLK
                     RVRYVLDPNDHGLSFDLTWTGAIPPLLEARHFIRSFERVTFDTERFAQTGFWAGTVTI
                     GDETVDVTGDRWRGTRDRSWGIRPVGEPEPPGIAAGAAPSMSMWNYAPMQFDDYSILY
                     ICSESPDGTRNLVDATRVWNDPDRPPEDLGRPEHAHEFRSGTRIVKHAVLSFPEALGG
                     PLDVDVTPLLEAHIGVGTGYGMDQDWRHGMYQGDLAVQGVDLDTVTNAERFFGIVDSA
                     ARFETNTGDVGYGLHEYMFLGPFPKYGLTGLLDGAP"
     gene            complement(3729..4370)
                     /locus_tag="VIJ44_07320"
     CDS             complement(3729..4370)
                     /locus_tag="VIJ44_07320"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY5165742.1"
                     /translation="MTAGLSDAKRRVLERLKRVEDATAAELAAALDLTEAGIRQHLDR
                     LERHGFVERRPVVQSPRGRPPGHWSLTPLAAELFPDRHADLTVELVDSIRAAVGEEGL
                     DHVIDARVERQHAAYQQVVPDATAPLRQRVAALARQRTAEGYMAEARADGDTVLLIEH
                     HCPVCRAAAACTGLCRGELELFRAVLGAEVDVERTQHLIAGDPRCVYRIRERS"
     gene            4444..6186
                     /locus_tag="VIJ44_07325"
     CDS             4444..6186
                     /locus_tag="VIJ44_07325"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEY5165743.1"
                     /translation="MPSENGNRVLAGSLVALQRDQQMVTKVGAWPVVVVWHEGRAYAI
                     EDRCPHMGFPLHRGTCEAGLLTCHWHHARFDLESGSTLDPWADDARAFDVELDGDEVW
                     VSVRSGGDDAARLQRRLREGLEDGLSLVVAKAVLGLLDAGVTPATLVQTGLEFGARNR
                     AAGWGAGLTVLVAVANVLADVAPADRAVALIHALVFVSNDTRGNPPHFGVPPLATDAL
                     TPARLAEWYRRFVDTRSADAAERVLTTALAEGTPVSDVEAMLFAAVTDHVFVDGGHAI
                     DFTNKAIEALGYAGPDIAPLILPTLVVGTTLSGRSEEGGAWRHPADLARLVRTANRAL
                     PDAVSSGLDRRGAFHDVRGLADRLLAEDPDAVVEALLDALRAGALEEQIGRAVAYAAS
                     LRVQRFHTQNDFGDWDTVHHTFTAANALHQGLARTPTVDLLRGAVHGALSVYLDRFLN
                     VPSARPPNAERGSLEALAACFDVQGAVDEAGQEAYGFLRGGGSRRDLVSTLAHLMLAE
                     DTGFHTYQHFEACVRQASAWPEGSEEGALILSGGARFLAAHAPTRRELPTVITIASRL
                     RRGEPLFEEASATE"
     gene            complement(6176..7309)
                     /locus_tag="VIJ44_07330"
     CDS             complement(6176..7309)
                     /locus_tag="VIJ44_07330"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEY5165744.1"
                     /translation="MARRAQLVSEDRAYAVRTVDEARATAARHVGDWRLDAAVTFGLP
                     EIDDRYHVWRVPVLDDATTRVGEIVIDARSAAVDAVRSTDAATVTARLALPDRESERP
                     APASPSRPISRSPLSNTVLCGDSELTLRALPPGSVDLVFTSPPYFNARPDYGDFVDYD
                     AYLDKMRAVLRECGRLLAEGRFLVINTAPVLLRRASRSQSSRRLAVPFDLHRILVEEG
                     FDFVDDIIWQKPEGAGWATGRGRRFAADRSPLQYKAVPVTEYVLVYRVHTDRLIDWNI
                     RAHHDPGAVDRSKIDGDYERTNVWRIPPASRRDHPAVFPQALAERVIRYWSFEDDVVL
                     DPFAGIGTVGVAAAALRRRFVLSEVDPGYVATMRRELTGSLIP"
     gene            complement(7370..7795)
                     /locus_tag="VIJ44_07335"
     CDS             complement(7370..7795)
                     /locus_tag="VIJ44_07335"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012398022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEY5165745.1"
                     /translation="MATVFTQVIDGELPGTFVWRDDRCVSFLSINPLRPGHALIVPRD
                     EVDHWIDLEPDLARHLMTVAQTVARAQQQAFQPSRVGLIIAGMEVPHTHLHVIPIDSE
                     ADLQFSNAARSVTPDELQETAAALRAALRADGATGVSDS"
     gene            7854..>8909
                     /locus_tag="VIJ44_07340"
     CDS             7854..>8909
                     /locus_tag="VIJ44_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY5165746.1"
                     /translation="MSAAPAPAAPAAADQDGFGELRACAAVPLPRLRSIPDFVGMPNG
                     DRPLADESTFWAAQGAALGWLLVCVVVTLLIGGGVASLLSWPYAVVLFGLFIWLPAYL
                     SAFRYATLFLEDVPALTGVRPSTPMTVVVSGSTAPASTLATLAHLAAQDYAGPVRVIL
                     VGGALADGDVHAVQRAASELRLDLDILRAGWLGSIEACNLVLPHVATTLMLALQAGAC
                     LHPSAVRLLVAHLESSPPETAAVSGHAFVRNRRCGARAEVLAADYAMDVDATQRIESL
                     YRGARVSEPACMLFQVAALRAVNGLPDREAGEVTVAWRFLARGWRVDHEPRAIAFTTE
                     PVTLGTAGRSRARSERGV"
BASE COUNT         1292 a         3053 c         3207 g         1357 t
ORIGIN      
        1 atagatcgcg ttcgacaggg tcacccaagc cgcctcgtgc tcgcggagga actcacgcgg
       61 cggcggcaag tacggccagg gcatgagatg gaaggagaag aagttcacgg cgacgtgacc
      121 gtatccggcg gagtaggttc ccggcgacgg ccgcgaccac ggaagagcgg tgtgaacgcg
      181 actccttcga cgcctgacgg ttcccgcccg gcctgtcgtc acgcgaggct gccgtgagcg
      241 ggcccgccgc cggtcggttc gtcggccaat cggtgccgcg ccgagaggat cctcgcctcc
      301 tcaccggcca cgggcagttc gtcgacgatg tcgtcgttcc cgggatgctg cacgcagcgt
      361 tcgtgcgcag cgatctggcc cgggggcgga tcaccagact cgacgtcgac gcggcgcgcg
      421 cgatgccggg agtccgggcc gtgctcaccg gagccgacct caacggcggt ctcgggtcga
      481 tgcagccgac gatgtttcag agcgatccgc caccttcgcc gtgcgtgccg ctccggccgc
      541 tcgcggagca cgacgtccgg ttcgtcggtg atccgatcgc gctcgtcatc gccgaaaacc
      601 ggtacctggc cgaagatgcc tgcgagctga tcgaagttga cctcgaggcc ctcccgcccg
      661 ttgtcgatcc cgaacgcgcc gtcgaggaga ccgacgagct cgtgcacccc gaactcggcg
      721 ccaatgtcgc ccagcgggtc gagtcgccgg ccgaccccga gctcgatgcg atcttcgaca
      781 cggccgcgca cgtcgtcacc gagacgatcg tccagcaccg tcatacgaac gttcccatgg
      841 agacccgggg ggtcgtggcc ggtcaccaac cggcgacggg cgagctcgat gtgtggatct
      901 cgacccagaa cccgcacgag gtccgccagg tttgtagtcg gatgctcagg gtccccgagc
      961 acctggtccg ggtccgcatg ggtgacgtcg gcggtggctt cggccagaag tacttcgcgc
     1021 cgcgcgacga gctggcggtc atcgccgcag cgcaccggct cggctgcacg gtcaaatgga
     1081 tcgaggaccg ccgcgagaac ctcatcgcgt cgaaccacgc tcgagcggac cggatgaccg
     1141 tgcgcatggc gctcgacgac accgggcaca tcctcggcgt ccatctggac cacctggagg
     1201 actgcggcgc gtatcccgtc ggcggaaccg ggggaatggg cccgttcgtc gcgatcctgt
     1261 ttcctggccc gtaccggatc ccgaagctcg ggtttcggag cacctcggtg tggacgaaca
     1321 cctgcggccg gggcgcgtac cggggaccgt ggatgatgga gaccgtcgcg cgtgaggaga
     1381 tgctggacct cgcggcccgc gccatcgggc tcgacccgct tgagctgcgg cgccgcaact
     1441 gcatcggacc cgacgagctc ccgttcacca ccgcgatggg catggtcctc gaccacgtca
     1501 ccccgtccgc gacgctcgag caggcggtcg agctcatcgg ctacgacgag ttccgcgctg
     1561 agcagcgacg cgccgccgcc gacgggcggc tgctcggcat cggcatcggc ctctacgtcg
     1621 agccaacctc gatcgcgtcc ggcaacatcg gcgtcgagac ggcgacgatc cgtgtccagc
     1681 cgagcggcac cgtgaccgtg cacctgggga cggggtccca cggccagagt gtcgagacca
     1741 cgatggcgca ggtcgtcgcc gagcacctcg gggtcgacta cgacgacgtc ctcgtcgtcc
     1801 aaggcgacac cgccagcgcc cccttcggcg gcggcaccgg tgggagccga accgcggtcg
     1861 tggccggcgg cgcggcgcgg gaagcgtcgc tccaagtgcg cgagaaggcg gtgcagatcg
     1921 cggcgcatct cctcgaggcc gcacccgagg atctcgagtc cggcgacggt cggctctcgg
     1981 tgcggggcac cccgacccgg ggggtgtcgc tcgccgaggt ggcacgggtg gcgttgaacg
     2041 cgccgctcga gctgccgccg gacatcgcac cgggtctcga agcgaccgtc agctaccagg
     2101 caccgccgat cacctggtcg aacgcctgtc atgtgtgcac cgtcgaggtc gatccggcca
     2161 ccggcaaggt cgaactgctg cgctacgtgg tgagtgagga ctgtggcgtc atgatcaacc
     2221 cgatggtcgt cgaaggccag atcgccgggg gtgtggtgca agggatcggc ggggtgctct
     2281 acgagcacct catctacgac gaggacggca acccgctcac gacgaccttc ctcgactacc
     2341 tggtgcccac cgcggcggaa gtcccagcga tcgaatacgg ccacgtcgag acgcgctccc
     2401 cgacgccggg ggggcacaag ggcatgggtg agggcggggc gattgggtca ccgccgtgtg
     2461 tgttcaacgc cgtcgccgac gcgctcgcgg cccggggcgc cgtcatcacc gagcagcccc
     2521 tcacgcccag ccgcatcctg gacgcgttga gccgctgacc cacgcggcgc cgcgacgcgg
     2581 cggtcgtggg tcgcggcgct agggggcgcc gtcgaggagg ccggtcagcc cgtacttggg
     2641 gaacgggccg aggaacatgt actcgtgcaa cccgtagccg acgtcgccgg tgttggtctc
     2701 gaaccgggcg gcggagtcga cgatcccgaa gaaccgctcg gcgttcgtca cggtgtcgag
     2761 gtcgacgccc tggacggcga gatcaccttg gtacatgccg tggcgccagt cctggtccat
     2821 cccatagccg gtgcccaccc cgatgtgggc ctcgagcagg ggggtgacgt cgacgtcgag
     2881 gggcccgccg agggcctcgg ggaaggacag caccgcgtgc ttcacgatcc gggtgccgga
     2941 ccggaactcg tgggcgtgct ccggtcgtcc gaggtcctcg ggaggccggt cggggtcgtt
     3001 ccaaacccgg gtggcgtcca ccaggttccg ggtcccatcg gggctctcgg agcagatgta
     3061 caggatcgag tagtcgtcga actgcatcgg cgcgtagttc cacatgctca ttgacggggc
     3121 ggctccggcg gcgattcccg gcggttcggg ctcgccgacg ggacggatac cccaggaccg
     3181 gtcccgggtg ccacgccagc ggtcgccggt cacgtcgacg gtctcgtcgc cgatcgtgac
     3241 cgtcccggcc cagaagccgg tctgggcgaa tcgctcggtg tcgaaggtga cacgctcgaa
     3301 cgatcggatg aagtggcgcg cttcgagcag cggagggatc gccccggtcc acgtcaggtc
     3361 gaacgagagg ccatggtcgt tggggtcgag cacgtaccgg actcgcttga gcccctcgag
     3421 gacctcgagt cggaacggtc cgaccgagag gttcatgcgg tcgacgccca gcgcacgcga
     3481 ggcgcgcacg acgcggtgcc gtgaccctcg ccgtacgagg gcgaacgcgt cctgcaccga
     3541 gaggttcggg tactggccca tgcccatgat gagcatcaag tcgtcgtcgg agccgtggag
     3601 catgaagtag tagcggtcgt agaagttccg gtccgacgtc gccgggtgcc gtatcacctc
     3661 agcggtttgg tgcacggggt agtcgtcgag tggtgacagc ggggaggtca tggccggaga
     3721 ctatgggatc aggaacgctc ccggatccgg tacacgcaac gcgggtcccc ggcgatgagg
     3781 tgctgggttc gctctacgtc gacctcggcg ccgagcacgg cacggaacag ctcgagctct
     3841 cctcgacaga ggccggtgca tgccgcggcg gctcggcaga ccgggcagtg gtgctcgatg
     3901 agcagaacgg tgtcaccgtc ggcgcgggct tccgccatgt agccctccgc ggtgcgctgg
     3961 cgcgccaacg ccgcgactcg ctgccgcagc ggtgccgtcg cgtcgggcac gacctgttgg
     4021 tacgccgcgt gttggcgctc gacgcgggcg tcgatgacgt ggtccaggcc ttcctcgccg
     4081 accgcggcgc ggatcgagtc gaccagctcc acggtcaggt cggcgtgacg gtcggggaag
     4141 agctccgccg cgagtggggt cagcgaccag tgcccgggcg gacgcccccg ggggctctgg
     4201 acgacggggc ggcgctcgac gaagccatgg cgttcgaggc ggtcgaggtg ctgtcggatc
     4261 ccggcctcgg tgaggtccaa ggccgccgcc agctcggcgg cggtcgcgtc ctcgacccgt
     4321 ttgagacgtt ccagtacccg ccgtttggcg tcgctgagcc ccgcggtcac ggcgacgacc
     4381 ctatccgagt tttcgcaagt gacctcttga cgaaatcgga agggaaggac attatgacaa
     4441 gcgatgcctt ccgaaaatgg gaaccgggtg ctggcggggt cgctcgtggc gctgcagcgc
     4501 gaccagcaga tggtcaccaa ggtcggtgcc tggccggtgg tggtggtctg gcacgagggg
     4561 cgggcctacg cgatcgagga ccgctgcccg cacatgggtt tcccactgca tcggggcacc
     4621 tgcgaagccg ggctgctgac gtgccactgg caccacgcgc gcttcgatct cgagtccggg
     4681 tcgacgctcg acccctgggc cgacgacgcc cgggccttcg acgtggagct cgacggcgac
     4741 gaggtctggg tgtcggtacg ctccggtggc gacgacgccg ctcgactcca gcggcgactc
     4801 agggaagggc tcgaggacgg cctgtcgctg gtcgtcgcga aggccgtcct cgggctgctc
     4861 gacgccggcg tcacacccgc gacgctcgtc cagaccggac tcgagttcgg tgcccgcaac
     4921 cgcgccgcag ggtggggcgc cgggctcaca gtgctcgtcg ccgtggcgaa cgtcttggct
     4981 gacgtggcgc ccgcggaccg tgcggtcgcg ctcatccacg cgctggtgtt cgtgtcgaac
     5041 gacacccgcg gcaacccgcc ccacttcgga gtgcctcccc tggcaactga cgcgctgaca
     5101 ccggctcgac tcgccgagtg gtaccgacgc ttcgtcgaca cccgctcggc ggacgcggcg
     5161 gaacgggtcc tgaccaccgc gctcgcggaa gggactccgg tctccgatgt cgaggcgatg
     5221 ctcttcgccg ccgtgaccga tcatgtcttc gttgacggtg gtcacgccat cgatttcacc
     5281 aacaaggcga tcgaggcgct cggctacgcg ggacccgaca tcgccccgct gatcctgccg
     5341 accctggtcg tcgggacgac gctcagtggg cgcagcgaag aaggcggcgc gtggcgccat
     5401 cccgccgacc tggcgcggct cgtgcgcacc gcgaaccgcg cgctgccgga cgccgtcagt
     5461 tccggcctcg atcggcgggg cgcgttccac gacgtgcgtg ggctcgcgga tcgccttctc
     5521 gccgaggatc cggacgcggt ggtcgaggcg ctgctcgacg cgctgcgagc cggcgccctc
     5581 gaggagcaga tcggtcgggc cgtcgcctac gcggcgtcgc tgcgggtgca gcggttccac
     5641 acccagaacg acttcggtga ctgggacacc gtccaccaca cgttcaccgc ggcgaacgct
     5701 ctgcatcaag gcttggcccg aaccccaacc gtcgacctcc tccgcggcgc cgtccacggt
     5761 gcgctgagcg tgtacctcga ccggttcctg aacgtgccgt ccgcccggcc gccgaacgcc
     5821 gagcgcggat ccctcgaggc cctcgcggcg tgcttcgacg tccaaggtgc cgtcgacgaa
     5881 gccggccagg aggcctacgg gttcctgcgc ggcggagggt cccgacggga cctggtctcg
     5941 accctcgccc acctcatgct cgccgaggac accggcttcc acacctacca gcacttcgag
     6001 gcctgcgtcc gccaggcctc ggcctggccc gagggctccg aggagggggc gctgatcctc
     6061 agtggcgggg ctcgcttcct cgcggcgcac gcgcccaccc ggcgcgagct gccgacggtg
     6121 atcaccatcg cgtcgcgttt gcgacgaggt gagcccttgt tcgaagaagc gtccgctacg
     6181 gaatgagtga gccggtcagc tctcggcgca tcgtggcgac gtatccgggg tcgacctcgc
     6241 tcagcacgaa ccgtcgtcgg agtgccgcgg cggcgacccc gacggtgccg atcccggcga
     6301 acgggtcgag caccacatcg tcctcgaagg accagtagcg aatcacccgc tcggcgagcg
     6361 cctgagggaa gaccgcgggg tgatcccttc ggctcgccgg tgggatgcgc cagacattgg
     6421 tgcgctcgta gtcaccgtcg atcttggagc ggtcgacggc gcccggatcg tggtgcgctc
     6481 ggatgttcca gtcgatgagc cggtcggtgt ggacccgata gaccaggaca tactcggtga
     6541 cgggtaccgc tttgtattgc agcggcgacc gatcggcggc gaaacggcgt cctcgcccgg
     6601 tcgcccagcc tgcgccttcg ggcttctgcc agatgatgtc gtcgacgaag tcgaacccct
     6661 cttcgacgag gatgcggtgc aggtcgaagg gaacggcgag ccgacgggac gactgggatc
     6721 ggctggcccg ccgcaacagc accggcgcgg tgttgatcac caggaagcgg ccttcggcga
     6781 ggagccggcc acattcgcga aggacggcgc gcatcttgtc gaggtacgcg tcgtagtcga
     6841 cgaagtcacc gtagtcgggt cgagcgttga aatacggcgg agaggtgaag accaggtcga
     6901 cgctgccggg agggagggcg cgcagggtga gctccgagtc gccgcacagc accgtgttcg
     6961 agaggggtga ccggctgatc ggtcgcgagg gtgaggccgg tgcgggacgc tcggactcgc
     7021 ggtcgggaag ggcgaggcgg gcggtcaccg tggcggcgtc ggtcgatcgg acggcgtcga
     7081 cggctgcgct gcgggcgtcg atcacgatct caccgacccg agtcgtggcg tcgtcgagga
     7141 ccggcacccg ccacacgtga tatcggtcgt cgatctcggg gagtccaaac gtcaccgccg
     7201 cgtcgagccg ccagtccccg acgtgccgtg ccgcggtggc gcgtgcctcg tcgaccgtac
     7261 ggaccgcgta cgcgcggtct tcgctgacca gctgcgcccg tcgcgccacg cgaagaccgt
     7321 accgggggac gcgggcgccg gtcgggcttt ctcccggtcc gacccgcacc tatgagtcgc
     7381 tgaccccggt cgcgccgtcg gcgcggagcg cggcacggag tgccgccgcg gtctcctgga
     7441 gctcgtcggg tgtcactgag cgggccgcgt tcgagaactg caggtcggcc tcggagtcga
     7501 tcgggatcac gtgcagatgc gtgtggggca cctccatccc ggcgatgatg agccccaccc
     7561 ggctcggctg gaacgcctgc tgttgggcgc gggcgacggt ctgcgccacc gtcatcaggt
     7621 ggcgagccag gtcaggttcg aggtcgatcc aatggtccac ctcatctcgg gggacgatca
     7681 gcgcatggcc gggccggagc gggttgatcg acaggaacga gacgcagcgg tcgtcgcgcc
     7741 acacgaacgt ccccggcagc tcgccgtcga tgacttgggt gaagacggtc gccacagccc
     7801 ggatggtagg cggcactgaa cttggggcgg ctggctgccg atacctgtcg gcgatgagcg
     7861 ccgcccccgc cccggctgca cccgcggccg ccgaccagga cggtttcggc gagctccggg
     7921 cctgcgctgc ggtcccgctg ccacggctcc gctcgatccc ggacttcgtc gggatgccga
     7981 acggcgaccg accgctcgcc gacgagtcga ccttctgggc cgcgcaaggc gccgcgctcg
     8041 gctggctctt ggtgtgcgtg gtcgtcacgt tgctgatcgg gggcggcgtg gcatcgctgc
     8101 tgtcgtggcc ctacgccgtc gtcctgttcg ggctgttcat ctggctcccg gcctacctca
     8161 gcgcattccg gtacgccacg ctgtttctgg aggacgttcc cgccctgacc ggggtgcgcc
     8221 cgagtacccc gatgaccgtc gtcgtctcgg gcagcaccgc gccggcatcg acgctcgcga
     8281 ccctcgctca cctcgcggcc caggactatg ccggcccggt ccgggtgatc ctcgtcggcg
     8341 gcgccctcgc cgacggggac gtgcacgcgg tgcagcgggc cgcgtcggag ctccgtctcg
     8401 atctcgacat cctccgcgcc ggatggctgg gatccatcga agcgtgcaac ctggtgttgc
     8461 cgcacgtcgc gacgacgctg atgctggcgc tccaggccgg ggcgtgcctg cacccgtcag
     8521 cggtgcggct gctggtggcg cacctcgagt cgtcgcctcc cgagaccgcg gcggtctcgg
     8581 gccacgcgtt cgtgcgcaac cgccgatgcg gcgcccgcgc cgaggtccta gccgccgact
     8641 acgccatgga cgtcgacgcc acccagcgca tcgagagcct gtatcgaggc gcgcgggtct
     8701 cggagccggc ctgcatgctg ttccaggtgg cggcgctgcg cgccgtgaac ggccttcccg
     8761 accgtgaggc cggtgaggtc accgtggctt ggcgattctt ggcccgcggt tggcgcgtgg
     8821 accatgagcc ccgcgccatc gcgttcacga ccgagccggt cacactggga acggcggggc
     8881 ggtcgcgggc ccgttctgag cgcggcgtc
//
LOCUS       DATCCH010000381         2586 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_438238, whole genome shotgun sequence.
ACCESSION   DATCCH010000381 DATCCH010000000
VERSION     DATCCH010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438238"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..2159
                     /gene="ftsH"
                     /locus_tag="VIJ44_07345"
     CDS             123..2159
                     /gene="ftsH"
                     /locus_tag="VIJ44_07345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEY5165747.1"
                     /translation="MSRPPSDMPPGRRPPGGPERRPGERPPPANPPPSWARMLPWLLI
                     LALVGVFALTNVFSGGNSSAKSLTYTQFLQAVDRGDVKKVTFNKSNGDITGTFSDSVS
                     GTKSFTSSGPTNDLQASTVKELQTKGVDFQYTQTSSNLFLSLLPLLLPVLLLIGFFVW
                     MARRQQGQMGAVMNIGRSRAKVYATEKPKTTFGDVAGYSAVKAEIAEVVDFLKYPQKF
                     KQIGARIPKGVLLVGPPGTGKTLIARAVAGEAGVPFVSVTGSDFMEMFVGVGAARVRD
                     LFQTARKQAPAIIFVDEIDSIGRKRGAGLGGGHDEREQTLNQMLAEMDGFEATEGIVM
                     MAATNRPDVLDPALLRPGRFDRTIVVPFPTQEERVAILKVHLRDKRVSSDLDVDIVAR
                     GTPGMAGADLANLVNEAALFAVRRGLSEIHMEDIEAARDRVLMGLKRDSMAINEEEKE
                     VIAFHEGGHAVLAYVLDHADPIHKVTILPTGMALGVTQQLPEEERHIYKREYIADSIV
                     VALGGRLAEEIVFGHVSTGAQNDLVRITELARKMVREWGMSERIGPMAWGAQGPVFLG
                     EDLVHTRDYSDDTARVIDEEVERILRQEESRARRILRLHRRGLEGVARALLEHETLDG
                     DEVSRIVDYAMGRKAGGFRKVKRADGSVMEVKPPAGNGGRKPRKAAARPRARGA"
     gene            complement(2166..>2586)
                     /locus_tag="VIJ44_07350"
     CDS             complement(2166..>2586)
                     /locus_tag="VIJ44_07350"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEY5165748.1"
                     /translation="LGVALGISWWLRRRRPEGPPRDAYPVPRQLDRADFARPDAGWLV
                     AYFSSEVCDSCQGLGPKVAALESAAVATCELDYTDHRDLHERYDISAIPMILLADAAG
                     VVHRAFIGATTATDLWAALAELRDPGSTPEPSLGQLD"
BASE COUNT          438 a          843 c          926 g          379 t
ORIGIN      
        1 gcgcgaccgc ccatcccgga gcggggcggt gggtccgagt cgcgtcccgg cggaccgggc
       61 cgtaccatgg gtctccggag gggtcgagca tcgcccgtcc gaagcccgac caggagtccc
      121 ccatgagtcg ccccccctcc gacatgccgc cgggccgccg tccccccggc gggcctgagc
      181 gtcggccggg cgaacggccg ccgccggcga acccgccgcc gtcgtgggcc cgcatgctgc
      241 cctggctgct gatcctcgcc ctcgtcgggg tcttcgcact caccaacgtg ttctcgggcg
      301 gcaacagctc agccaagagc ctcacctaca cccagttcct ccaggccgtg gatcgtggcg
      361 acgtcaagaa ggtcacgttc aacaagtcga acggggacat caccgggacg ttcagcgatt
      421 cggtgagcgg cacgaagagc ttcacgagca gcggcccgac caatgatctg caggcgagca
      481 cggtcaagga gctgcagacc aagggcgtcg acttccagta cacccagacc agctcgaacc
      541 tcttcttgag cctgctgccc ttgctcttgc cggtgctcct cctcatcggg ttcttcgtgt
      601 ggatggcccg ccgccaacag ggccagatgg gcgcggtgat gaacatcggg cggagccggg
      661 cgaaggtcta tgcgaccgag aagcccaaga cgaccttcgg cgacgtcgcc gggtacagcg
      721 cggtgaaggc cgagatcgcc gaggtcgtcg acttcttgaa gtacccccag aagttcaagc
      781 agatcggggc ccgcatcccg aagggggtgc tcctggtcgg tccgcccggg acgggcaaga
      841 ccctgatcgc ccgggccgtg gccggagagg ccggcgtgcc gttcgtgtcg gtgaccggct
      901 cggacttcat ggagatgttc gtcggtgtcg gtgcggcgag ggtccgtgat ctgtttcaga
      961 ccgctcgcaa gcaggctccg gcgatcatct tcgtcgacga gatcgattcg atcggccgca
     1021 agcgtggcgc cggcctcggt ggcgggcacg acgagcgcga gcagaccctt aaccagatgc
     1081 tcgccgagat ggacggcttc gaggcgaccg aaggcatcgt gatgatggcg gcgacgaacc
     1141 gtcccgacgt gctcgacccg gcgttgcttc gaccagggcg cttcgaccgc acgatcgtcg
     1201 tgccgttccc gactcaggag gagcgcgtcg cgatcctcaa ggtgcacctg cgcgacaagc
     1261 gcgtcagctc cgatctcgac gtcgacatcg tggcgcgcgg caccccgggg atggcgggcg
     1321 ccgatctcgc caacctggtg aacgaagccg cgctgttcgc ggtgcggcgc ggcctgtccg
     1381 agatccacat ggaagacatc gaagccgcgc gcgaccgggt gctgatgggc ctcaaacggg
     1441 actcgatggc gatcaacgaa gaggagaagg aggtcatcgc cttccacgaa ggcggtcacg
     1501 ccgtccttgc ctatgtcctc gatcacgcgg acccgatcca caaggtgacg atcctcccca
     1561 ccgggatggc gctcggggtg acgcaacagc tccccgaaga ggaacgccac atctacaagc
     1621 gggagtacat cgcggactcg atcgtggttg cgctcggagg tcggctcgcc gaggagatcg
     1681 tgttcgggca cgtgtcgacc ggtgcccaga acgacctggt gcgtatcacc gagctggccc
     1741 gcaagatggt gcgcgaatgg ggcatgagcg agcggatcgg tccgatggcg tggggcgcgc
     1801 agggtccagt tttcctcggt gaggaccttg tgcacacgcg ggattactcc gacgacacgg
     1861 cgcgtgtcat cgacgaagaa gtcgagcgca tcttgcgtca agaagagagc cgggcccggc
     1921 ggatcctgcg cttgcatcgg cgggggctcg aaggcgtcgc ccgggcgtta ctggagcacg
     1981 aaaccctgga cggcgacgag gtgtcgcgca tcgtggacta cgcgatgggg cgcaaggccg
     2041 gcgggttccg caaggtcaag cgcgctgacg gcagcgtgat ggaggtcaag cctccggccg
     2101 gcaacggggg ccgcaagccg agaaaggctg cggcccggcc ccgggcccgg ggcgcgtagc
     2161 ccacttcagt cgagctggcc gagggaaggt tcgggcgtcg agcccgggtc gcgcagctcc
     2221 gcgagagctg cccacaagtc ggtggcggtc gtcgcgccga tgaaggcgcg gtgcaccaca
     2281 ccggcggcgt cggcgagcag gatcatcggg atcgccgaga tgtcgtagcg ctcgtggaga
     2341 tcgcggtggt cggtgtagtc cagctcgcac gtcgccacgg ccgccgattc gagtgccgcg
     2401 accttaggcc cgaggccctg acaggaatcg cacacctccg acgagaagta cgcgacgagc
     2461 caccccgcgt cgggccgagc gaagtcggcg cggtcgagct gacgggggac ggggtaggcg
     2521 tcgcgggggg gtccttcggg tcggcggcgg cgaagccacc acgagatgcc cagtgcgacg
     2581 cccagg
//
LOCUS       DATCCH010000382         3529 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_605204, whole genome shotgun sequence.
ACCESSION   DATCCH010000382 DATCCH010000000
VERSION     DATCCH010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605204"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="VIJ44_07355"
     CDS             <1..434
                     /locus_tag="VIJ44_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine racemase C-terminal domain-containing
                     protein"
                     /protein_id="HEY5165749.1"
                     /translation="PAPILADRVSLTPVLSLRAQVTYVKTLPAGTRVSYGLRYELTRR
                     SRIVTVPVGYADGVPRNLGLRGGEVLIGGRRYPIAGTVTMDQLMVDVGDATAEVGDEV
                     VLLGRQGGDEVTAEEWAERLDTIAYEIVTGIGPRVPRRYVG"
     gene            431..1438
                     /locus_tag="VIJ44_07360"
     CDS             431..1438
                     /locus_tag="VIJ44_07360"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY5165750.1"
                     /translation="MSTRHRALKTVGVAAGVAAAAAGSAYVAQRAALAAIRRRSDPDA
                     GTLGPLEFDEARRLPSHDGGTLYTAVRGSGVPILLSHGVTITSRVWVKQFRSLPLRGM
                     QVIAFDHRGHGESTVGDSGHSVENLAADLRTVIEALDLHDAILVGHSMGGVAVQAFAL
                     RHPDVLHERVRGLVLASTLAKTSVSASRQLRWLAERVTGGFDLRKVMARPDLGSMFAR
                     IGFGREPLASHVELNREMLASCDSVTAREATTALLGLDLTAELSSLDIPTLVVGGTAD
                     VITPPAESRRLANLIPGARLHLFEGAGHMLMLERTEAFDDLLIDFARDLGVLPAAAGA
                     A"
     gene            1435..2337
                     /locus_tag="VIJ44_07365"
     CDS             1435..2337
                     /locus_tag="VIJ44_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEY5165751.1"
                     /translation="MTGSITDVPGLRVGHRARGGTGVTVVLVPPGTVGSGELRGGGPA
                     TRDLALLEPSRTVEHVDAVVLTGGSAFGLAVADGVVDWLAGQGRGYPTRAGPVPIVPT
                     AAIYDLVESGGTRPGPEDGRLAAEAAGADPVLVTGRVGAGTGATVGKWRGAEHAVPGG
                     LGSASAKVGDATVGALAVVNALGDVHDAAGNLLAGSRAPSDAASFPDPTPFDGPADPN
                     TTLVVVATDARCSKADCWLLAQSAQHGIVRAVHPSHTRHDGDLAIVLATGAVDAHLDR
                     LRVAATDVVAAAVRNAVTSGDDGS"
     gene            2337..2933
                     /locus_tag="VIJ44_07370"
     CDS             2337..2933
                     /locus_tag="VIJ44_07370"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEY5165752.1"
                     /translation="MTTLDPSPGWRTFDELATTASTCTKCPLAETRTQVVFGVGNPDA
                     GLVFVGEGPGAEEDRQGVPFVCRAGQLLTRLIEGIGLTRDEVYICNVVKCRPPGNRDP
                     LPAEIEACRPYLEAQLDFLDPRVVVTLGNFATRLLLGTKQGITKLRGTEFPYRDERAV
                     LIPTLHPSAVLRNGGAALAQARADFVLVKRTLARARSA"
     gene            2930..3415
                     /gene="tsaE"
                     /locus_tag="VIJ44_07375"
     CDS             2930..3415
                     /gene="tsaE"
                     /locus_tag="VIJ44_07375"
                     /inference="COORDINATES: protein motif:HMM:NF014426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEY5165753.1"
                     /translation="MSDVTVVANTAEQTRTIGRCIAPLLRAGDVVVVAGGLGAGKTTF
                     TQGLAAGLGVTDPVVSPTFTLTREYEGRLRLVHVDLYRLDRAQEVLDLGLEDVADGAV
                     LVVEWGDVAAAYLTPEHLEVHLAADIDAGSDDERTIAVTFRGRSWMPRANAVAGAIGA
                     A"
BASE COUNT          477 a         1299 c         1229 g          524 t
ORIGIN      
        1 ccccggcccc gatcctcgcc gatcgcgtct cgctcacccc ggtcctctcg ctgcgggcgc
       61 aggtcaccta cgtcaagacc ttgccggccg ggacccgcgt ctcctacgga ctccggtacg
      121 agctcacgcg tcgctcgcgc atcgtgaccg tcccggtcgg ctacgccgac ggggttcctc
      181 ggaacctcgg gttgcgcggc ggtgaggtgc tgatcggggg gaggcgctat ccgattgcgg
      241 ggaccgtgac gatggaccag ctcatggtcg acgtcggcga tgccaccgcc gaggtcggag
      301 acgaggtcgt gctccttggg cgccagggcg gtgacgaggt gaccgccgaa gagtgggcgg
      361 agcggctcga caccatcgcc tacgagatcg tgaccggaat cgggcctcgg gtcccgcgac
      421 ggtacgtcgg atgagcaccc gccatcgcgc gttgaagacg gtcggtgtgg ccgcaggtgt
      481 cgccgcggcg gcggccggtt cggcgtacgt cgcgcaacga gccgcacttg ccgcgatccg
      541 gcgccgctcc gaccccgacg ccgggacgct cggtccgctc gagttcgacg aggcccgccg
      601 actccccagc cacgacggcg ggaccctgta caccgcggtc cgcgggagcg gggtgccgat
      661 cctcttgtcc catggggtca cgatcacctc gcgagtctgg gtcaagcagt tccggtcgct
      721 tcctcttcgc ggcatgcagg tcatcgcgtt cgatcatcgg ggtcacggcg aatcgacggt
      781 cggggacagc ggccactcgg tggagaacct cgcggccgat ctgcgcaccg tgatcgaggc
      841 cctcgacctt cacgacgcga tcctggtggg ccactcgatg ggaggcgtcg cggtgcaggc
      901 cttcgcgctc cgccatcccg acgtcctcca cgaacgggtt cgcggtctgg tgctggcgtc
      961 cacactcgcc aagacgagcg tctccgcctc ccgccagctg cgttggctcg cggaacgcgt
     1021 caccggcggg ttcgacctgc gcaaggtcat ggcccgcccg gacctcgggt cgatgttcgc
     1081 gcgcatcggt ttcggccgtg agccgctcgc cagccacgta gagctcaacc gggagatgct
     1141 ggccagctgc gacagcgtca ccgcccgcga ggccacgacc gcattgctcg gcctcgacct
     1201 gaccgccgag ctgtcgagcc tcgacatccc aactctggtc gtcgggggca ccgcagacgt
     1261 gatcacaccg ccggcggagt ctcggcgtct agcgaatctc attcccggag cgcggctgca
     1321 cctcttcgaa ggcgcggggc acatgctcat gctcgagcgc accgaggcgt tcgacgacct
     1381 gctgatcgac ttcgcccgcg acctcggagt gctgccggct gcggccggtg ctgcatgacc
     1441 ggatcgatca ccgacgtgcc gggcctccga gtcgggcacc gggcccgggg cggcaccggc
     1501 gtcacggtcg tcctcgtccc gccgggaacc gtcggctcgg gagagctgcg cggcggtggc
     1561 cccgcgaccc gggacctcgc actgctcgaa ccgagccgaa cggtggagca cgtcgacgcg
     1621 gtggtcttga ccggcgggtc agcgttcggg ctcgcggtcg ccgacggagt cgtcgactgg
     1681 ctcgccgggc agggacgggg ctacccgact cgggccggcc cggtcccgat cgtcccgacc
     1741 gccgcgatct acgacctcgt cgagtccgga gggactcgac ctggccccga ggatggtcgc
     1801 ctcgctgccg aagcagccgg cgcggacccg gtgctggtca ctggtcgcgt gggggcgggt
     1861 acgggcgcca cggtgggaaa gtggcgcggc gccgagcacg ctgtgcccgg gggactgggc
     1921 tcggcgagcg cgaaggtcgg cgacgcgacg gtgggggcgc tcgcggtcgt caacgccctc
     1981 ggcgatgtcc acgacgcggc cggcaacctc ctcgcggggt cccgggcacc gagcgacgcg
     2041 gcgtcgttcc ctgaccccac cccgtttgac ggtcccgcag atccgaacac gacgctcgtg
     2101 gtcgtcgcca ccgacgcccg ctgctccaaa gcggactgtt ggctgctcgc ccagagcgct
     2161 cagcacggaa tcgtccgggc cgtgcatccc tcacacaccc gccacgacgg ggatctcgcc
     2221 atcgtgctcg ccaccggggc cgtcgacgcc cacctcgacc ggctgcgggt cgccgccacc
     2281 gacgtcgtcg cggcggcggt gcgcaacgcg gtcacctccg gcgatgatgg atcgtgatga
     2341 cgacgctcga cccgtccccc gggtggcgga ccttcgacga gctggccacc accgcgagca
     2401 cctgcaccaa gtgccccctg gccgagaccc gcacccaggt cgtcttcgga gtcgggaacc
     2461 ccgacgcggg tcttgtgttc gtcggcgagg ggccgggcgc cgaggaggat cgacaagggg
     2521 tccccttcgt ctgtcgagcc ggccagctgc tcacccggct catcgagggg atcgggctca
     2581 cccgcgacga ggtgtacatc tgcaacgtcg tgaagtgccg cccaccgggg aaccgggacc
     2641 cgctcccggc cgagatcgaa gcgtgtcggc cctacctcga ggcgcaactc gacttcttgg
     2701 atccccgggt cgtcgtcacg ctcgggaact tcgcgacccg tctcctgctc gggaccaagc
     2761 agggcatcac caagctgcgg ggcacggagt tcccgtaccg cgatgagcgt gccgtcctga
     2821 tccccaccct gcacccctcg gcggtcctgc gaaacggcgg cgccgcgctc gcgcaggctc
     2881 gggcggactt cgtactcgtc aagcgcacgc tggcgcgcgc tcgtagcgcg tgagcgacgt
     2941 caccgtggtg gccaacaccg cggagcagac ccgcacgatc ggacgctgca tcgcgccgct
     3001 gcttcgcgcc ggtgatgtcg tcgtcgtcgc cggtggcctc ggcgccggga agacgacgtt
     3061 cacgcagggt ctcgccgccg gcctcggcgt gaccgacccg gtcgtgagcc ccacgttcac
     3121 cctcacccgc gagtacgagg gtcgactccg gctcgtgcac gtcgacctgt accgactcga
     3181 ccgggcccag gaggtgctcg acctcggcct cgaggacgtc gcggacggcg cggtcctcgt
     3241 cgtcgagtgg ggtgacgtcg ccgcggccta cctgaccccc gagcatctcg aggtgcacct
     3301 cgccgctgac atcgacgccg gttccgacga cgaacgaacg atcgcggtca ccttccgggg
     3361 ccgatcctgg atgcctcggg ccaacgccgt cgccggtgcc attggggccg cctgatgctc
     3421 ctgctcgcca tcgacacctc caccccccgc gtcgcggtcg cgattggtga caccggccgg
     3481 gtccgtgccg aggtcggcct caccggtcgg cgacggcacg cggagcagc
//
LOCUS       DATCCH010000383         3485 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_146385, whole genome shotgun sequence.
ACCESSION   DATCCH010000383 DATCCH010000000
VERSION     DATCCH010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146385"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="VIJ44_07380"
     CDS             <1..541
                     /locus_tag="VIJ44_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011774193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEY5165754.1"
                     /translation="PEPLRPAQYILKARLQLMRDMGAPVSPFNAFLFLQGLETLSLRM
                     ERHCQNAQAVAEWLEARDEVEWVAYPGLQSSPWAARADKYLPRGKGAILAFGIKGGLE
                     AGKRFIEGLELFSHLANVGDVRSLAIHPASTTHSQLVPEEQAAAGVTPDLVRLCIGIE
                     TVDDILADLEAGFRSAKGA"
     gene            606..1730
                     /locus_tag="VIJ44_07385"
     CDS             606..1730
                     /locus_tag="VIJ44_07385"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020461770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEY5165755.1"
                     /translation="MSSPFGAWQPGDEPGRRRFVSVFGDRPHALEAGGRLEGVQVAYE
                     TWGELAPDRSNAVLVVHALTGDSHAAGPAGPGHPQPGWWDPIIGPGAALDTSRYFVVC
                     PNVLGGCQGTTGPASLAADGRPYGSRFPTITIRDQVVLEAALADRLGIERWHAVVGGS
                     MGGMRVLEWAVGFPDRVRRAVVISVGAKATAEQIAWCSVQIRAIRADPNWQNGNYGAG
                     TPGDGPHTGLSIARGIGQISYRSELEFDQRFGRDHQGDENPLAGGRYAVESYLDYHGE
                     KLTRRFDANSYIVLSQAMNHHDVGRGRGGVAAALSRVSAETTLAGISSDRLYPLRLQH
                     ELAELIPTAGDVEVIESIHGHDGFLVESDAVGKVVGRALS"
     gene            complement(1747..2346)
                     /gene="recR"
                     /locus_tag="VIJ44_07390"
     CDS             complement(1747..2346)
                     /gene="recR"
                     /locus_tag="VIJ44_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013599734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEY5165756.1"
                     /translation="MYEGPVQTLIDELGRLPGIGPKSAQRIAYYLLKAAPDDANRLAK
                     AITEAKARVSWCRRCFNVAQGELCGFCRDDRRDAALLCVVEEPRDIVAVERTHEFSGR
                     YHVLQGAISPIEGIGPEQLRVKELLRRVGDESVTEVILATNPNIEGEATAMYLAKLLQ
                     PLGVTVTRLASGLPVGGDLEYADEVTLGRALEGRREVGA"
     gene            complement(2366..2668)
                     /locus_tag="VIJ44_07395"
     CDS             complement(2366..2668)
                     /locus_tag="VIJ44_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEY5165757.1"
                     /translation="MRQMQRMQEDMASAQATLAAQTVEGSAGGGMVKATATGSGELRA
                     VSIDAEVVDPEDVEMLEDLVVAAVSEALRAAQELQTTTMGSVTGGLDLGSLGGLLG"
     gene            complement(2908..>3485)
                     /locus_tag="VIJ44_07400"
     CDS             complement(2908..>3485)
                     /locus_tag="VIJ44_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165758.1"
                     /translation="PPPAPRVPGRTIGAVRASGDSPPPTVRPEPPPPVVDPVPSAGDD
                     GGPLELDDVIVAWGELLPGFSPATRAAVQSAHPTRVDDDVVTFGVDPALLEAARPRFK
                     KEADTIRAALAARLGRSVKFNLVASEEFSAVGDATRPPPVERAHTEAPPAEAEPEPED
                     LADASDAPPRRPEERLTEALGATVVEEVPRD"
BASE COUNT          493 a         1232 c         1218 g          542 t
ORIGIN      
        1 ccccgagccg ctccgcccgg cgcagtacat cctcaaggcc cggctccagc tcatgcgcga
       61 catgggcgca ccggtgtcgc cgttcaacgc gttcttgttc ttgcagggcc tcgagacgct
      121 gtcgttgcgg atggaacgtc actgccagaa cgcccaggcg gtcgccgagt ggctcgaagc
      181 ccgcgacgag gtcgagtggg tcgcctaccc gggcctgcag tcgagcccgt gggcggcgcg
      241 cgccgacaag tacctcccgc ggggcaaggg cgcgatcttg gccttcggga tcaagggcgg
      301 cctcgaagcg ggcaagcggt tcatcgaggg ccttgagctg ttcagccatc tcgccaacgt
      361 cggcgacgtc cgcagcctcg cgatccaccc ggcgtcgacc acccactccc aactcgtacc
      421 cgaagagcaa gccgcggcgg gggtcacccc cgacctggtg cggctctgca tcggcatcga
      481 gaccgtcgac gacatcctcg ccgacctcga ggccggcttc cggtcggcga agggagcatg
      541 accccagcgc tgatcaccgc aacgatgacg cgccacccga cgacgcggtc gctcaccggg
      601 ccctgatgag ctcgccgttt ggtgcctggc agccgggcga cgaacccggc cggcgccggt
      661 tcgtgtccgt gttcggcgac cgcccgcacg ccctcgaggc cgggggtcgc ctcgaagggg
      721 tccaggtcgc gtacgagacc tggggtgagc tcgctcccga ccgatccaac gcggtgctcg
      781 tcgtccacgc cctgaccggt gacagccacg ccgccggacc cgccggcccc ggtcatcccc
      841 aacccggatg gtgggatcca atcatcgggc ccggtgccgc gctcgacacg tcgcgctact
      901 tcgtcgtgtg cccgaacgtg ctgggaggct gccagggcac gaccggtccc gcgtcgttgg
      961 cggcggacgg ccggccgtac ggctcccggt tcccaaccat caccatccgc gaccaggtcg
     1021 tgctcgaagc cgcactcgct gaccggctcg gcatcgaacg ctggcacgcc gtcgtcggcg
     1081 gatcgatggg cggcatgcgg gtgctcgaat gggcggtcgg cttcccggac cgggtccggc
     1141 gggcggtggt gatctcggtc ggcgcgaagg cgaccgcaga acagatcgcg tggtgttcgg
     1201 tgcagatacg cgccatccgg gccgacccga actggcagaa cggcaactac ggcgccggaa
     1261 caccgggcga cggaccccac accggattgt cgatcgctcg cggcatcggc cagatcagct
     1321 accggtcaga gctggagttc gaccagcggt tcggacgcga ccaccaaggt gacgagaacc
     1381 ccctcgccgg cggccgctac gcggtcgagt cctacctcga ctatcacggc gagaagttga
     1441 cgcgtcggtt cgacgccaac tcctacatcg tgttgagcca ggcgatgaac caccatgacg
     1501 tcggccgcgg gcgcggtggc gtcgccgccg cgctcagccg ggtgagcgcc gagaccacct
     1561 tggccgggat ctcctcggac cgcctctacc cactgcgact ccaacacgag ctggccgagc
     1621 tcatcccgac ggccggcgac gtcgaggtga tcgaatcgat ccacggccac gacggcttcc
     1681 tcgtcgagtc cgacgcggtg ggcaaggtcg tcggacgcgc gttgtcctga cgacgtctcg
     1741 gcacggtcag gcgccgacct cgcggcgacc ctcgagggcg cggcccaggg tcacctcgtc
     1801 cgcgtactcg aggtccccgc cgacggggag cccactggcg aggcgggtga cggtcacccc
     1861 gagcggctgg agcagcttgg cgaggtacat cgcggttgct tcaccctcga tgttcgggtt
     1921 ggtggcgagg atcacctcgg tgacagattc gtcgccaacg cgtcgcagca gctccttgac
     1981 gcgcagctgc tcgggaccga tcccctcgat cggggagatc gctccttgca agacgtggta
     2041 gcggccgctg aactcgtggg tgcgctccac ggcgacgatg tcgcggggtt cttcgacgac
     2101 acagagcaac gcggcgtcgc gtcggtcgtc gcggcagaac ccgcagagct caccctgggc
     2161 gacgttgaag caacgccgac accaactcac tcgggctttc gcctcggtga tggctttcgc
     2221 cagccggttc gcgtcgtcgg gcgcggcttt caacaggtag taggcgatgc gctgcgcgga
     2281 cttgggtccg atccccggga gtcggcccag ctcatcgatg agggtctgga ccgggccctc
     2341 gtagatattc atggtgggtt ctgcctcagc cgagcaggcc gcccagggac ccgaggtcca
     2401 ggcctccggt caccgaaccc atggtggtgg tttgcagctc ttgggccgcg cgtagcgcct
     2461 cgctcaccgc ggcgaccacc aggtcctcga gcatctcgac gtcctcgggg tcgacgacct
     2521 cggcgtcgat ggacacggcc cgcagctcgc cgctgccggt cgcggtcgcc ttgaccatcc
     2581 cgcccccggc ggatccctcg acggtctggg ccgctaacgt cgcttgcgcg ctcgccatgt
     2641 cttcttgcat gcgctgcatc tggcgcatca ggttcgcctg cgggttgccg ggcggcttct
     2701 gcttcgccac gaacactcct cgttcgggta gtgggtcagt tttcggcccg ttgcgtacgt
     2761 tcggctttac gtcggcagcc cgcaggcagt ttcacgcccc ctcctaccga gggaccgacg
     2821 gctctcgcaa ggtccgaagg tacccacggg ctgtgacgct caaacggcgt tggtgggcca
     2881 gtttggatgc cggctgaccc accaccccta gtcgcggggg acttcctcga cgacggtagc
     2941 gccgagggcc tcggtgagcc gctcctccgg tcggcggggt ggcgcgtcgg acgcgtcggc
     3001 gaggtcctcc ggctcgggtt cagcctccgc gggtggtgcc tcggtgtggg cccgttcgac
     3061 cggcggcggg cgggtggcgt caccgacggc gctgaactcc tccgacgcga ccaggttgaa
     3121 cttcacgctg cgtccgaggc gggcggcgag ggcagcgcgg atggtgtccg cctccttctt
     3181 gaaccgggga cgggctgcct cgaggagggc cggatcgacg ccaaacgtga ccacgtcgtc
     3241 gtcgacccga gtcggatgcg cggactgcac cgcggcacga gtcgcgggcg agaacccggg
     3301 gagcagctca ccccaggcga cgatcacgtc gtcgagctcc agtggtcccc cgtcgtcgcc
     3361 cgcggagggc accgggtcga cgacgggcgg gggcggctcg ggtcggaccg tcgggggtgg
     3421 cgagtcgccg ctggcccgca ccgcgccgat cgtgcggccg gggacccgcg gcgccggcgg
     3481 tggcg
//
LOCUS       DATCCH010000384         3841 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_22043, whole genome shotgun sequence.
ACCESSION   DATCCH010000384 DATCCH010000000
VERSION     DATCCH010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22043"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /gene="pdxT"
                     /locus_tag="VIJ44_07405"
     CDS             complement(<1..444)
                     /gene="pdxT"
                     /locus_tag="VIJ44_07405"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEY5165759.1"
                     /translation="MKVGVLALQGAFREHREILDALGVDTVEVRSPASLGAVDALILP
                     GGESTTIGQLLVTSGLLPALEERVADGLPVLGTCAGLILLATDVLDGRPDQLTLAALD
                     ATVRRNGYGTQLQSFEADLQVAGLTGPFPGVFIRAPQLERTGPDVE"
     gene            complement(475..1368)
                     /gene="pdxS"
                     /locus_tag="VIJ44_07410"
     CDS             complement(475..1368)
                     /gene="pdxS"
                     /locus_tag="VIJ44_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020572254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEY5165760.1"
                     /translation="MADPRPDRHGTARVKRGLAEMLRGGVIMDVVTPEQARVAEDAGA
                     VAVMALERVPADIRRDGGVARMSDPTMVEGIQQAVTVPVMAKCRIGHFAEAQVLEALG
                     VDYIDESEVLTPADEAHHVDKWAFTIPFVCGATNLGEALRRVAEGAALIRSKGEAGTG
                     NIVEAVRHLRAIFGDIRRLTVADEPERYAAAKELRAPIDLVTEVAERGELPVPLFCAG
                     GIATPADAALVMQLGAQSVFVGSGIFKSADPAVRAKAIVEATTHYADAGIVAKVSRGL
                     GEAMRGEEISTLDVKLAERGW"
     gene            complement(1426..2412)
                     /locus_tag="VIJ44_07415"
     CDS             complement(1426..2412)
                     /locus_tag="VIJ44_07415"
                     /inference="COORDINATES: protein motif:HMM:NF022702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HEY5165761.1"
                     /translation="MTRHCAATLAALAVLTASTGTAVTVRAEPLAAASNVAAAPLTGR
                     PDPTGVARRRPALAVKIENTPDARPQAGLDTADVVYEEQVEGGITRFWAVFNSVSPGD
                     VGPIRSVRAMDPNIVTPLHGIAAFSGGTLSNETLIRRAPVTWVDQTNAGPAFYRTPTR
                     AAPHNLYAHTALLWERPARPGPPPPLFHYRDTVAPHRGTPASTVTLGFAAPYNVTYTY
                     QAPTHTWMRSYGTTPHRAASGRPLAPTNVIIQFVTYGSGGDAQLLGDAPDQPAWVLRD
                     GTLITGRWAKPDPATPTQFTDPSGAPIALRPGPTWVELLPASASVRTTTTTR"
     gene            complement(2409..3347)
                     /locus_tag="VIJ44_07420"
     CDS             complement(2409..3347)
                     /locus_tag="VIJ44_07420"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEY5165762.1"
                     /translation="MSQGPPPLQPLAHTDFYREIRRNKLRTAVILFSFIALIAVAGIA
                     INFLLGFGVGGVIVALVIAGILGFVSYYNSDKVALAAARARPADGPEYQRYHNLVEGL
                     CIAAGLPKPRLYVVDDPAPNAFATGRNPKHAALAVTTGLLQEMDRVELEGVIAHELSH
                     VKNYDILASTVAVIAVGAVALLANIGLRFWIFGGNREGDDGGLGIILAIASIALLIFA
                     PIAAQMMQFAMSRKRELLADATGVQLTRYPPGLCSALKKLQTDQAVIRHANHATAQLW
                     IESPLDRNQDTKGSWLNRAFDTHPPLDERIKILEAM"
     gene            complement(3381..>3841)
                     /locus_tag="VIJ44_07425"
     CDS             complement(3381..>3841)
                     /locus_tag="VIJ44_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEY5165763.1"
                     /translation="AWSQIDVQLKRRYDLIPNLVETVKGYAAHERQTFEAVTNARSQA
                     MNAQQGGDVAQQAQAENTLSGALKSLFAVAEAYPDLKANQNFLQLQEELTSTEDRIAY
                     ARQFYNDSVLSYNNKIQVFPRNLLASSFNFVKREYFQAEPEATDPVKVQF"
BASE COUNT          531 a         1141 c         1539 g          630 t
ORIGIN      
        1 ttcgacgtcg ggtccggtgc gttcgagctg gggggcacgg atgaacaccc cggggaaggg
       61 tccggtgaga ccggccacct ggaggtcggc ctcgaaggac tggagctggg tgccgtagcc
      121 gttgcggcgc acggtggcgt cgagcgcagc gagggtcagc tggtcgggcc ggccgtcgag
      181 gacgtcggtg gcgaggagga tgagaccggc gcaggtaccg aggacgggga ggccgtcggc
      241 aacccgctct tcgagcgcgg ggagcagccc ggacgtcacg aggagctggc cgatggtggt
      301 cgactcgccg ccggggagga tcagcgcgtc gacggcgccg aggctggccg gtgaccggac
      361 ctcgacggtg tcgacgccga gggcgtcgag gatttcacga tgctcgcgga acgccccttg
      421 gagcgcgagg actccgacct tcatgacctg gggggcgtgc gtctgcccgc acgcctacca
      481 gccccgctcg gcgagtttca cgtcgagggt gctgatctct tcgccacgca tggcctcgcc
      541 gagcccgcgg gacactttgg ccacgatgcc ggcgtcggcg tagtgggtgg tggcttcgac
      601 gatcgccttg gcccggacgg cggggtcggc gctcttgaag atgccgctgc cgacgaacac
      661 cgattgggct ccgagctgca tcacgagcgc ggcgtcggca ggggtcgcga tccctccggc
      721 gcagaagagc gggacgggga gctcgccgcg ctccgcgacc tcggtgacga ggtcgatcgg
      781 ggcccgcagc tctttagccg cggcgtagcg ctcgggttcg tcggcgacgg tgagccggcg
      841 gatgtcaccg aagatggccc gcagatgccg gacggcttca acgatgttgc ccgtcccggc
      901 ttcgcccttg gagcggatga gggccgcgcc ctcggcgacg cggcgaagag cttctccgag
      961 gttggtggcc ccgcagacga aggggatggt gaaggcccac ttgtcgacgt gatgggcctc
     1021 gtcggcgggg gtgagcacct cgctctcatc gatgtagtcg accccgaggg cttcgaggac
     1081 ctgggcctcg gcgaagtggc cgatgcggca cttggccatg acggggacgg tgacagcctg
     1141 ctggatcccc tcgaccatgg tggggtcgct catgcgggcc acgccgccgt cgcggcgaat
     1201 gtcggccggg acgcgttcga gggccatgac cgcgaccgcg ccagcgtctt cggcgacgcg
     1261 ggcctgctcg ggggtgacga cgtccatgat gacgccgcca cggagcattt cggccaggcc
     1321 ccgcttgacc cgggcggtgc cgtgacggtc ggggcggggg tcggccacgg ccgcaagtgt
     1381 accggccgtc cccgacgaac cggccggggt tgtcgtcgcg ggccgctatc gggtggtggt
     1441 ggtggtacgg accgacgcgc tggcgggaag cagctcgacc caggtggggc cgggacgaag
     1501 ggcgatgggc gcgccggacg ggtcggtgaa ttgggttggg gtggcgggat ccggtttcgc
     1561 ccagcgcccg gtgatgaggg tgccgtcgcg gagcacccag gccggctggt cgggggcgtc
     1621 gccgagcagc tgggcgtcgc cgccgctgcc gtaggtgacg aactggatga tcacgttggt
     1681 gggtgcgagg ggccgtccag acgcggcccg gtggggcgtg gtgccgtagg agcgcatcca
     1741 ggtgtgggtc ggggcctggt aggtgtacgt gacgttgtac ggcgccgcga acccgagcgt
     1801 gacggtgctc gcgggggtgc cccggtgagg cgcgacggtg tcgcggtaat ggaacagcgg
     1861 cggcgggggg ccggggcggg ccggtcgttc ccagaggagc gcggtgtggg cgtagaggtt
     1921 gtgaggagcg gcgcgggtcg gggttcggta gaacgcgggg ccggcgttgg tctggtcgac
     1981 ccaggtgacg ggcgcgcgcc ggatcagggt ctcgttcgag agcgtgcctc cggagaaggc
     2041 cgcgatcccg tggagggggg tcacgatgtt ggggtccatg gcgcgcaccg agcggatcgg
     2101 cccgacgtcg ccgggtgaga cggagttgaa caccgcccag aagcgggtga ttccgccttc
     2161 gacctgttcc tcgtagacga cgtcggcggt gtcgaggccg gcttggggcc gcgcgtcggg
     2221 ggtgttctcg atcttgaccg cgagcgcggg tcggcgacgg gcgaccccgg tcgggtcggg
     2281 ccggccggtg agcggcgccg ccgcgacgtt cgaggcggcg gcgagcggct cggcccggac
     2341 ggtcacggcg gtcccggtgc tggcggtgag gacggcgaga gccgcgaggg ttgcggcgca
     2401 gtgccgcgtc acatcgcttc gaggatcttg atgcgctcgt cgaggggtgg gtgggtgtca
     2461 aacgcccggt tgagccagga gcccttggtg tcctggttgc ggtcgagcgg cgactcgatc
     2521 cagagctgag cggtggcgtg gttcgcgtgg cggatgactg cctggtcggt ctggagcttc
     2581 ttgagcgccg agcacagccc cggtgggtac cgggtcagct gcaccccggt ggcgtccgcg
     2641 agcagttccc gcttgcggct catcgcgaac tgcatcatct gcgccgcgat gggggcgaag
     2701 atcagcaacg cgatcgaggc gatcgcgagg atgatcccga ggcctccgtc gtcgccttcg
     2761 cggttcccgc cgaagatcca gaagcgcagc ccgatgttgg cgagcagcgc gaccgcgccg
     2821 accgcaatga cggcgaccgt ggaggcgagg atgtcgtagt tcttcacgtg ggatagctcg
     2881 tgggcgatga cgccttcgag ctcgacgcgg tccatctcct ggaggaggcc ggtcgtgacc
     2941 gccaacgcgg cgtgcttggg attgcgcccg gtggcgaacg cgttcggtgc cggatcgtcg
     3001 acgacgtaca gcctcggctt tgggagcccg gcggcgatgc agaggccctc gacgaggttg
     3061 tggtaccgct ggtactcggg accgtcggcg ggtcgggcgc gtgccgcggc gagcgccacc
     3121 ttgtcggagt tgtagtacga gacgaacccg aggatcccgg cgatgacgag cgcgacgatc
     3181 accccgccga caccgaaccc gaggaggaag ttgatcgcga tgccagccac cgcgatcaac
     3241 gcgatgaacg agaacaggat caccgcggta cggagcttgt tgcggcgaat ctcgcggtag
     3301 aagtcggtgt gcgcgagcgg ttggagcggt ggcggacctt gactcatgtt cgaactcgga
     3361 gtggtgtcag gtgcagccgg tcagaactgg accttgaccg ggtcggtggc ctcgggctcg
     3421 gcttggaagt actcgcgttt cacaaagttg aagctgctgg cgagcaagtt ccgcgggaac
     3481 acctggatct tgttgttgta gctgaggaca ctgtcgttgt agaactgccg cgcgtacgcg
     3541 atgcggtcct cagtggaggt cagttcttcc tggagctgca ggaagttctg gttcgctttc
     3601 agatccgggt acgcctcggc gaccgcgaag aggctcttca gggcgccgct gagcgtgttc
     3661 tcggcttggg cttgctgcgc gacgtcgccg ccttgctggg cgttcatcgc ctggctgcga
     3721 gcgttcgtga ccgcctcgaa cgtctggcgc tcgtgggcgg cgtagccctt gaccgtctcg
     3781 accaggttgg ggatcaggtc gtagcgccgc ttgagctgca catcgatctg ggaccaggcg
     3841 t
//
LOCUS       DATCCH010000385         2544 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_605844, whole genome shotgun sequence.
ACCESSION   DATCCH010000385 DATCCH010000000
VERSION     DATCCH010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605844"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1435
                     /locus_tag="VIJ44_07430"
     CDS             <1..1435
                     /locus_tag="VIJ44_07430"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEY5165764.1"
                     /translation="NGDDVIGKDGIEQVFESELRGTPHTRKLEVDSRGRIVRVISDTP
                     AKTGNDLKLTMDVNIQKVAEDSLQQGILQVRGMRDTAFKNQLKNFSAPGGSAIVLDAR
                     DGSVVAMASNPTFDVTKFTSGVPVDEFKTLTDPASNFPLLNRATQGLYPPGSTFKLIT
                     AIAALEHGEAIPGTAGYNFVDNGCLNFGNPPQQFCNAGKTPHGNVDLPHAIEVSSDVY
                     FYNLGFKFWRTLQGSNATNPPTPGNAKDGYGIQTVARQFGFGRSTTVGLPQEASGRIP
                     DQAFKQAINKHNPDPFTRQWLPGDSANVATGQGDVLVTPLQLARAYATFANGGTLTSP
                     RLASEVLAPGDHTTIVRDLPVQQDAKINLKPDVRATILSGLSGAVNASDGTAKAAFNG
                     YTGPPLAGKTGTAQQPPPQEDTAWFVGIINPDPTDPKQPQYVVVVNVEQAGFGGTVAA
                     PIARRIIDALNGNLNPAPVHVAQPQSD"
     gene            1435..>2544
                     /locus_tag="VIJ44_07435"
     CDS             1435..>2544
                     /locus_tag="VIJ44_07435"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEY5165765.1"
                     /translation="MSVSGRNRRYMQAATYTPRARRRTILDADLVARLDWILIGACVA
                     IVMIGILMIYSASRNIVSGDPAYFVKRQLLALTIGLVIAMVLLRIDYRKFRDFSLIGY
                     VLTVAMLFFVLSPIGSASKGHQAWYQLPFGFQFQPSEFAKFGLIVALAGYVNEHRDEI
                     DPWRLSVIVGLALVPIGLVQLQPDLGTNMVLIFAVIGLLAVAGVRGRYLVALALLGMT
                     LVFAVVSVGILQKYQVDRLTSVFDTSAQAKQGIAYNQDQSKKTISTGGFAGLGLFKGP
                     QTRLGFVPEQQTDFIFTAVGEELGFVGGATLLALFAIVLWRTWRTAQLAADLYGTLVC
                     TGVLAVFAFQIFENIGMTMGIMPVTGIPLPFMSYGG"
BASE COUNT          449 a          927 c          751 g          417 t
ORIGIN      
        1 caacggcgat gacgtcatcg gcaaggacgg catcgagcag gtcttcgaga gcgagctgcg
       61 cggcacgccc cacacccgca agctggaagt cgatagccgc ggtcgaatcg tgcgggtcat
      121 cagcgacact cccgccaaga ccggcaacga cctcaagctc acgatggacg tcaatatcca
      181 gaaggtcgcc gaagactcgt tgcagcaggg gatcctccag gtccgcggca tgcgcgacac
      241 cgcgttcaag aaccagctca agaacttctc ggcgcctggc ggatcggcca tcgtgctcga
      301 cgcccgagac ggttccgtcg tcgcgatggc ctcgaacccg acgtttgacg tcaccaagtt
      361 caccagcggc gtcccggtcg acgagttcaa gaccctcacc gatccggcca gcaacttccc
      421 gttgctgaac cgtgccaccc agggtctcta ccccccgggg tcgacgttca agctcatcac
      481 ggcgatcgcc gcgctcgagc acggcgaggc gatccccggg acggccggct ataacttcgt
      541 cgacaatggc tgcctgaact tcgggaaccc gccccagcag ttctgcaacg ccggcaagac
      601 cccgcacggc aacgtggacc tcccgcacgc catcgaggtc tccagcgacg tctacttcta
      661 caacctcggc ttcaagttct ggcgaacctt gcagggctcc aacgcgacca acccgcccac
      721 accgggcaac gccaaggacg gctacgggat ccaaaccgtg gctcgacagt tcggcttcgg
      781 acgctccacc acggttggcc tgccccaaga ggccagcggc cgcatccccg accaggcgtt
      841 caagcaggcg atcaacaagc acaacccgga cccgttcacg cgacagtggc tgcccggtga
      901 cagcgccaac gtcgcgaccg gccagggtga cgtcctcgtc accccgctcc agctcgcccg
      961 tgcgtacgcg acgttcgcca acggcgggac cctcacctca cctcgcctgg cgagtgaggt
     1021 gctcgccccc ggcgaccaca ccaccatcgt ccgggaccta cccgttcagc aggacgccaa
     1081 gatcaacctc aagcccgacg ttcgggccac gatcctgtcc ggcctctccg gcgcggtgaa
     1141 cgccagcgac gggacggcca aggccgcgtt caacgggtac acgggtccac cgctcgccgg
     1201 caagaccggc accgcccagc agcccccacc ccaggaggac acggcctggt tcgtggggat
     1261 catcaaccct gaccccaccg atccgaagca gccgcagtac gtggtcgtcg tgaacgtcga
     1321 gcaagccggc ttcggcggca ccgtcgcggc gcccatcgcc cggcgcatca tcgacgcact
     1381 caacggcaac ctcaacccgg cgccggtcca cgtcgcgcaa ccccaaagcg actgatgagc
     1441 gtctccggtc gcaaccgccg gtacatgcag gccgcgacgt acacgccacg agcccggcgc
     1501 cgaacgatcc tcgacgccga cctcgtggcg cggctcgact ggatcctgat cggcgcgtgc
     1561 gtcgcgatcg tcatgatcgg gatcctcatg atctactcgg cgtcgcgcaa catcgtcagc
     1621 ggcgatccgg cgtacttcgt gaagcgtcag ctcctcgccc tcaccatcgg tctggtcatc
     1681 gccatggtgc tgttgcgcat cgactatcgg aagtttcggg acttctcgct gatcggctac
     1741 gtgctcacgg tcgcgatgct gttcttcgtc ctgtcaccga tcggctccgc cagcaagggc
     1801 caccaggcct ggtatcagct tcccttcggg ttccagttcc agccgtcgga gttcgccaag
     1861 ttcgggctga tcgtggccct cgccggctac gtgaacgagc accgcgatga gattgacccg
     1921 tggcgcctca gtgtcatcgt cgggttggcc ctcgtcccga tcgggttggt ccagctccaa
     1981 cccgacctcg ggaccaacat ggtgctgatc ttcgccgtca tcggcctgct cgcggtcgcg
     2041 ggtgtccgcg gccggtacct cgtcgccctc gccctcctcg gcatgacgct ggtcttcgcg
     2101 gtcgtcagcg tcgggatctt gcagaagtat caggtcgacc ggctcaccag cgttttcgac
     2161 accagcgccc aggccaagca gggcatcgcg tacaaccagg atcaatccaa gaagacgatc
     2221 agcactggtg gattcgccgg cttggggctc ttcaagggtc cccagacccg gctcgggttc
     2281 gtcccagagc agcagaccga cttcatcttc accgcggtcg gcgaggagct cggcttcgtc
     2341 ggcggcgcga ccctgctcgc cctcttcgcc atcgtgctgt ggcggacctg gcgaaccgcc
     2401 cagctcgccg ccgacctgta cgggacgctg gtctgcaccg gtgtcctcgc ggtcttcgcc
     2461 ttccagatat tcgagaacat cgggatgacg atgggaatca tgccggtgac ggggatccca
     2521 ttgccgttca tgagctacgg cggg
//
LOCUS       DATCCH010000386         3546 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_22324, whole genome shotgun sequence.
ACCESSION   DATCCH010000386 DATCCH010000000
VERSION     DATCCH010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22324"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /locus_tag="VIJ44_07440"
     CDS             <1..829
                     /locus_tag="VIJ44_07440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY5165766.1"
                     /translation="ARGAVQTVSLVANPDGGRSAGLNRALHRARAAMVVRVDARSVPP
                     PDYMARCVARLAANPAIGVVGAIQRPATRDPGAGARGIARALRNPWLLGAAPYRRAGG
                     GGPADTAYLGAFRRHELLARGGYDERLHANEDFDLCARYRAAGQQVWIEPGLEVEYEA
                     RRTYRELWAQYRAFGEAKVSYWRATGERPNRRQVVALGGALTAVLAGLVVGRRPWRLV
                     TALVAALGVLALVDHSVDPEDRDVGTRVAAFGAYATFLTGWCCGIVRGLARRADPWD"
     gene            complement(826..2166)
                     /gene="nadA"
                     /locus_tag="VIJ44_07445"
     CDS             complement(826..2166)
                     /gene="nadA"
                     /locus_tag="VIJ44_07445"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEY5165767.1"
                     /translation="MLVETSGFALLADPGGEGTLRSGGMIAHGPAWNTAPQIDSGADN
                     PAVGDQAVPAGHYRSRQPERGSTLGRGGINMLRLQAPLPDRYTRASADELDGWIAAAK
                     SQLGSRLLVLGHHYQRPEVIRWADERGDSFKLAQFAAANEQATDIVFCGVHFMAESAD
                     ILTGDHQRVILPDLNAGCSMADMADIDQVLEAWDEIAAVTDIERIMPITYMNSSADLK
                     AFVGEHGGAVCTSSNARIILEWAFQQADKVLFFPDQHLGRNTGYEMGYDNSHMRVWDP
                     RRDLGGLDEPDVKEATFLLWKGHCTVHQRFQPTHVDAFRAEHPAGEVIVHPECCHEVV
                     AMADRVGSTERILEWVAAAPSGATIGVGTEIHMIQRLAADHPDKTVVSLDPLVCPCST
                     MFRIDGPHLAWCLETLAAGEIVNQIVVDDRTAELARLALQRMLDLPGIPTDTVD"
     gene            2151..3383
                     /locus_tag="VIJ44_07450"
     CDS             2151..3383
                     /locus_tag="VIJ44_07450"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEY5165768.1"
                     /translation="MFLRAPINLADRRRAGTSRAVLILAVLLIAARVAAVPIALGQDA
                     TKGRRKVLTGDVSRYHAIAVHRRTPYRDFSVEYPPLMLGAIEALDGGTLRSATVATMW
                     SQLLLDLGVVALMAWGWGRRAALAYLIIGLPFVFYPFLYLRLDLLSVALAVGGLALVR
                     RRRPALGGATLALACFAKVWPVLLAPGLLVRRSWRALVAFAAVGVAGMVAWVAWAGTD
                     APIQVLSMRGATGWEIESSVGAVVRAVTGDRVHIDHGAWRTGVMPGWASPLLAVLLFA
                     AVVATWLLADRNRTHGARVIEGTAALAAIGSFLLFSPLLSPQFVLWLVPFAAIVAARG
                     DWAIGRLTVLVVALSVVDLNVIQELVHTNSWWPQAIVLVRNVLLVILVGKCLVLLVRG
                     ARRPAMGLAAPQTVEAAA"
BASE COUNT          438 a         1219 c         1309 g          580 t
ORIGIN      
        1 ggcccgcggc gcggtgcaga ccgtcagcct cgtcgcgaac cccgacggtg ggcgcagcgc
       61 cggactgaac cgggcgctgc accgagcccg ggccgcgatg gtggtgcggg tcgacgcccg
      121 gtcggtgccg ccccccgact acatggcccg ctgcgtcgcc cgcctggccg cgaaccccgc
      181 catcggtgtc gtcggcgcga tccaacgacc cgcgacccgc gacccgggag cgggcgcgcg
      241 cggcatcgcc cgggcgttgc ggaacccgtg gctgctcggc gccgccccct atcggcgggc
      301 gggcggaggg ggaccggcgg acaccgcgta cctgggcgcg ttccgacgcc acgaactgct
      361 cgcccgcggc gggtacgacg agcgtctcca cgccaacgag gacttcgacc tgtgtgcccg
      421 ctaccgtgcc gcgggccagc aggtctggat cgagcccggt ctcgaggtcg agtacgaggc
      481 gcgccgcacc taccgggagc tctgggccca gtaccgggcg tttggtgagg ccaaggtcag
      541 ctactggcgc gcgacggggg agcgtcccaa ccgacgccaa gtcgtcgcgc tcggcggggc
      601 gctcaccgcg gtgctcgcgg gcctggtggt cggtcgccgt ccgtggcggc tcgtcaccgc
      661 gctcgtcgcg gcgctcgggg tcctcgcact cgtcgaccac tcggtcgatc ccgaagaccg
      721 cgacgtcggc acccgggtcg ccgccttcgg ggcgtacgcg acgttcttga ccgggtggtg
      781 ctgcgggatc gtgcggggct tggcgcgccg cgctgaccct tgggactagt cgacggtgtc
      841 ggtcgggatg ccgggaaggt cgagcatccg ttgcaacgcc aggcgggcca gctccgcggt
      901 ccggtcgtcg acgacgatct ggttcacgat ctcaccggcg gcgagggtct cgaggcacca
      961 ggcgaggtgc gggccgtcga tgcggaacat cgtcgagcac gggcacacca gcgggtcgag
     1021 cgacacgacg gtcttgtcgg gatggtctgc cgcgagccgc tggatcatgt ggatctcggt
     1081 gccgacaccg atggtggcgc cgctcggcgc ggcggcgacc cactcgagga tccgctcggt
     1141 cgagccgacc cggtcagcca tcgccaccac ctcgtggcag cactccggat gcacgatcac
     1201 ctcgccggcg ggatgctcgg cccggaacgc gtcgacgtgc gtgggttgga agcgctgatg
     1261 gacggtgcag tgacccttcc agagcaagaa ggtggcttcc ttcacgtcgg gttcgtcgag
     1321 tcccccgagg tcacgacgcg gatcccagac ccgcatgtgc gagttgtcgt agcccatctc
     1381 gtagccggtg tttcggccga ggtgctggtc gggaaagaac aacaccttgt cggcctgctg
     1441 gaacgcccac tccaagatga ttcgcgcgtt ggaggaggtg cagaccgcgc cgccgtgctc
     1501 gccgacgaag gccttcaggt ccgccgatga gttcatgtag gtgatcggca tgatgcgttc
     1561 gatgtcggtg accgcggcga tctcgtccca ggcttcgagg acctggtcga tgtcggccat
     1621 gtcggccatc gagcagccgg cgttcaggtc cgggaggatg acccgctggt ggtcaccggt
     1681 gaggatgtcg gcggactcgg ccatgaagtg cacgccgcag aagacgatgt cggtcgcctg
     1741 ctcgttggcg gcggcgaatt gggccagctt gaacgagtcg ccccgctcgt cggcccagcg
     1801 gatcacctcg ggtcgctgat agtggtggcc gaggaccagg agccgggagc cgagctggct
     1861 cttcgcggcg gcgatccagc cgtcgagttc gtcggcggac gcccgggtat accggtccgg
     1921 cagcggagcc tggagtcgca gcatgttgat ccctccccgg ccgagggtgg atcctcgttc
     1981 gggctggcgg ctccggtagt ggccggcggg gacagcctgg tcgcccaccg cggggttgtc
     2041 ggccccggag tcgatctggg gcgcggtatt ccaggccggc ccgtgcgcga tcataccgcc
     2101 cgaccgcagc gtcccctccc ccccggggtc ggcgagcagg gcaaaccccg atgtttctac
     2161 gagcacctat caacctcgct gaccgccgcc gggctgggac gtcgcgtgcc gtcctcatcc
     2221 tggcggtgct gctcatcgcg gcccgggtag cggcggtccc gatcgcgctc ggccaggacg
     2281 ccaccaaggg tcgccgcaag gtgctgaccg gcgacgtgag ccgataccac gccatcgcgg
     2341 tgcatcgccg caccccgtat cgggacttct cggtggagta cccgccgttg atgctcggcg
     2401 ccatcgaggc cctcgacggt gggacgttgc gcagcgcgac ggtggcgacg atgtggtccc
     2461 agctgctgct cgacctcggc gtcgtcgcgc tgatggcctg gggttggggg cgacgggctg
     2521 cgctcgccta tctcatcatc ggtttgccgt tcgtgttcta ccccttcctc tatcttcgcc
     2581 tcgacctgct gtcggtggcg ttggcggtcg gcgggttggc gctcgtgcgg cgtcgacgac
     2641 cggcgttggg gggcgcgacg ctcgccctcg cctgcttcgc gaaggtctgg ccggtgctgc
     2701 tcgcgccggg cctgctggtc cgacggtcgt ggcgggccct ggtcgcgttc gccgcggtcg
     2761 gggttgcggg catggtggcc tgggtggcct gggcggggac cgacgccccg atccaggtgc
     2821 tgtcgatgcg cggcgccacc ggctgggaga tcgagagcag cgtcggggcc gtcgtccggg
     2881 ccgtgaccgg tgatcgggtc cacatcgacc atggcgcgtg gcggaccggg gtcatgcccg
     2941 ggtgggccag cccgctcctc gcggtgctgc tcttcgccgc ggtggtcgcc acctggctcc
     3001 tcgccgaccg gaaccgtacc cacggtgccc gcgttatcga gggcaccgcg gcgctggccg
     3061 cgatcggatc cttcctgctc ttctcgccgc tgctgtcccc ccaattcgtg ctgtggctcg
     3121 tcccgttcgc ggcgatcgtc gccgctcggg gggattgggc gatcggccgg ctgactgtcc
     3181 tggtggtggc gctcagcgtc gtggacctca acgtgatcca ggagctggtg cacaccaaca
     3241 gctggtggcc ccaggcgatc gtgctggtcc gcaacgtgct gctcgtgatc ctggtcggga
     3301 agtgtctcgt gctgctggtc cgcggcgccc gccggccggc gatgggcctg gccgcacccc
     3361 agacggtgga agcggcggca taaccgtccg ggaccgccac aatggggtgg tggtgcaacg
     3421 ccccgagacc ggctcgatcc ccgacacgcc cggctcgtac cagttccagg acgcccaggg
     3481 gcgggtcctc tacgtgggga aggccaagag cctccgcagc cggctggcca gctacttcgc
     3541 gccacc
//
LOCUS       DATCCH010000387         2045 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_231737, whole genome shotgun sequence.
ACCESSION   DATCCH010000387 DATCCH010000000
VERSION     DATCCH010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231737"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VIJ44_07455"
     CDS             <1..250
                     /locus_tag="VIJ44_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007677152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY5165769.1"
                     /translation="AAGPIGCAAVDIGRLLGEGLARADHLTRAGIRDALERVKRLPAT
                     CGQPGTTLSFGHYDHGALHGDYLVLRTWAGGRSVQVST"
     gene            376..540
                     /locus_tag="VIJ44_07460"
     CDS             376..540
                     /locus_tag="VIJ44_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165770.1"
                     /translation="MAATALTTPTSEAAAPVSWEPCNEGRSGDFGAGCCDACGWPLDD
                     HTPSDAARAA"
     gene            complement(543..1406)
                     /locus_tag="VIJ44_07465"
     CDS             complement(543..1406)
                     /locus_tag="VIJ44_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEY5165771.1"
                     /translation="MPSTIWTGSISFGLVAVPVRVISATKSLDVRFNQLEEGTNARIR
                     YRKISEATGEEVSNAQIQKGYEISPGQYVVVDDDEMQALAPKSSRMIEIEDFVDLDDI
                     DPLYFEQPYYLTPDATALKPYKLLVEAMGELRKVAIGRVVMRSKERLVAIRPLDGVLC
                     LETMRYADEVLAREGLVPDDEGAEPSERELAMARQLVDALADGFDPDRYHDEYREQLM
                     DLIQRKAAGEQIVAEPTVEEPGKVLDLMAALEASLARTGTVEATSDAASDEPGPAKPT
                     KKRAARSRRSA"
     gene            1426..>2045
                     /locus_tag="VIJ44_07470"
     CDS             1426..>2045
                     /locus_tag="VIJ44_07470"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEY5165772.1"
                     /translation="MAVGSKAVIEVEGRHLTLTNLEKVLYPDAAFTKAAVIDYYARIA
                     PVMLGHLGGRAVTMVRFPNGVDGGSFFEKRCPPHAPDWVRRAPMDGLVACVVDDAPTL
                     VWMANLAALELHTLQARVDDPEHPTSMLFDLDPGAPAGVLTCCQVALDLRSALEGLGL
                     VSVIKTSGSKGLHLAVPVRGATADDTKSFARGLGQLLAKQEPDRVTV"
BASE COUNT          303 a          778 c          627 g          337 t
ORIGIN      
        1 cgcggccggc cccatcgggt gcgccgcggt ggacatcgga cggctcctcg gcgaaggtct
       61 cgctcgcgct gatcacctca ctcgggccgg gatccgcgac gccctcgagc gggtcaagcg
      121 gctccccgcc acctgcggtc agcccggcac cacgctgagc ttcgggcact acgaccacgg
      181 cgcgctccac ggcgactacc tcgtgctgcg aacctgggcc ggcggccgca gcgtccaggt
      241 ctccacctga cccacccgcc ggcgttcccc accgggcctc ccggccgcgg gcgtcacacc
      301 ctcatggtct cctcagaggg tcgccggccc cgcccgacgt cgaattacac tgatgtcatt
      361 ctgggaagga ccaccatggc cgccaccgcc ctcacgaccc cgacctccga ggccgccgcc
      421 cccgtctcct gggagccctg caacgaggga cgatcgggcg acttcggcgc cggctgctgc
      481 gacgcttgcg gctggcccct cgatgaccac acccccagcg acgccgcccg cgccgcctga
      541 gctcaggccg acctccgcga ccgagcggct cgcttcttcg tcggcttggc gggccccggc
      601 tcgtccgaag cggcgtcgct cgtcgcctcc accgtcccgg tacgagccag gctcgcttcg
      661 agggcagcca tcaggtccaa gaccttgccc ggctcctcca ccgtcggttc ggcgacgatc
      721 tgctcacccg ctgctttgcg ctggatgaga tccatgagct gctcgcggta ctcgtcgtgg
      781 taccggtcgg gatcgaaccc gtcggcgagc gcgtccacca gttggcgggc catcgccagt
      841 tcgcgttcgg acggctcggc cccctcgtcg tcgggaacca gtccctcgcg ggccaggacc
      901 tcgtcggcgt agcgcatcgt ctcgaggcac aagaccccat ccaacggtcg gatggcgacc
      961 agacgttcct tcgagcgcat caccacccgc ccgatcgcca cttttcggag ctcgcccatc
     1021 gcctcgacca gcagcttgta cggcttcaac gccgtggcat ccggggtgag gtagtacggc
     1081 tgctcgaagt acaacgggtc gatgtcatcg aggtcgacga agtcctcgat ctcgatcatc
     1141 cggctcgact tcggggccag cgcctgcatc tcgtcatcat cgacgaccac gtactggccc
     1201 ggggagatct cataaccctt ctgaatctgg gcgttcgaga cctcttcgcc ggtcgcctcg
     1261 gagatcttgc gataccggat ccgagcgttc gtaccctcct cgagctggtt gaaccgcacg
     1321 tcgaggctct tggtggccga gatcaccctc acgggcaccg cgaccagccc gaagctgata
     1381 ctccctgtcc agatcgtgct cggcatccca ctaggttcgc agagcatggc cgtcggatcc
     1441 aaagcggtca tcgaggtcga ggggcgccac ctgaccctga ccaacctcga gaaggtcctg
     1501 taccccgacg cagcgttcac caaggccgcg gtcatcgact actacgcgcg gatcgcgccg
     1561 gtgatgctcg gccatctggg cggccgggcg gtgacgatgg tgcggttccc gaacggcgtc
     1621 gacggtggct cgttcttcga gaaacgctgc ccaccccacg cccccgactg ggtgcggaga
     1681 gccccgatgg acggcctcgt cgcgtgtgtc gtcgacgatg cccccacgct ggtctggatg
     1741 gccaatctcg ccgcgctcga gctccacacc ctccaagcgc gagtcgacga ccctgagcat
     1801 ccgacatcga tgttgttcga cctcgatccc ggagcaccgg ctggcgtcct cacctgctgt
     1861 caggtcgccc tcgacctgcg atcggccctc gagggtctgg gtctcgtcag cgtcatcaag
     1921 acctccggga gcaagggcct gcacctcgcg gtcccggtgc gcggcgccac cgccgacgac
     1981 accaagtcgt tcgcgcgcgg cctcggccag ctgctcgcca agcaggaacc cgaccgcgtg
     2041 acggt
//
LOCUS       DATCCH010000388         2070 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_355658, whole genome shotgun sequence.
ACCESSION   DATCCH010000388 DATCCH010000000
VERSION     DATCCH010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355658"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="VIJ44_07475"
     CDS             <1..570
                     /locus_tag="VIJ44_07475"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY5165773.1"
                     /translation="PTVGNVHEVAQAVNPEARIVYVDHDPVAVTHSRALLGGNDRATA
                     IADDIREPRLVLQHAVATGLLDLGRPVAVLFIAVLPFIDDADRPAELIAEYMSATAPG
                     SYLAVSHPLRGDQLGTRDLAKVYDQSRSPNALRFRSRAQIEAMFGDLTVVDPGVVLMP
                     LWRPELTDDAEAPEVDADYPGLAGLGRRD"
     gene            648..>2070
                     /locus_tag="VIJ44_07480"
     CDS             648..>2070
                     /locus_tag="VIJ44_07480"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY5165774.1"
                     /translation="MERTSLISMSGRSLLPFLRGLAATLLDALTADELDPARPYGIGV
                     ELVEAHFTEVAALERTLAVLGAQLIDRAAARPGGAARLAEIMGAVAAGYAQGLQDRTR
                     TEQERITASAFAARVSAEEARWNSEARLEAVFAESVIGIVISEIDGRILEVNRALCEM
                     LGYTAVEITGRTFWEFVHPDDAPGFGEHVEDLVAGTTNHLRVEKLYSRKDGAAIWTDL
                     VLSLIRDPDEKPRYVVAMIENITERYHLQTDLQHQAQHDPLTGLPNRTVFFQRLDAAL
                     LTGEQVGICYLDLDGFKIINDTLGHDQGDEMLRAVAHRLMTDLGREGHLVARMGGDEF
                     VVLVEHGAQGERLQRVAQIALDSIRRPTLLGGHDVVVSASVGIVQSGDGGVGAAQLMK
                     AADTTLYWAKADGGDRYVLFDAERHRADVARFALSARIPEALADGEFVVEYQPLVRLR
                     DRQTIGVEALVRWKLPTGEQLPPG"
BASE COUNT          325 a          669 c          749 g          327 t
ORIGIN      
        1 cccacggtcg gaaacgtgca tgaggtggcc caggcggtca acccggaggc aaggatcgtc
       61 tacgtcgatc acgacccggt cgcggtcacc cacagccgag cactgctggg cgggaacgac
      121 cgcgcgacgg cgatcgccga cgacatccga gagcctcgcc tggtgctcca gcacgcggtg
      181 gccaccgggc tgctggatct gggccggccc gtcgcggtgc tgttcatcgc ggtgctgccg
      241 ttcatcgacg acgcggatcg gccggccgag ctgatcgcgg agtacatgtc cgcgacagcg
      301 cccgggagct acctcgcggt gagccatccg ctgcgcggtg accagctggg gacgcgggac
      361 ctcgcgaagg tctacgacca gtcccgctcg ccgaacgcct tgcggttccg gtcccgggcc
      421 cagatcgagg cgatgttcgg tgacctgacg gtggtcgacc cgggcgtggt gctgatgcca
      481 ctctggcggc cagagctgac cgatgatgcc gaagcaccag aggtcgatgc ggactatccc
      541 ggtctcgcgg gcctggggcg acgagactga ccgggtacgc cgaacggacc ggcagctcgg
      601 acgcgctgcc caccgcggat cagctcgccc gcgtctggaa gcggggtgtg gagcgaacca
      661 gcctgatctc gatgagcggg cggagcctgc taccgttcct tcggggtctg gccgccacgc
      721 tcctggatgc gctgacggcc gacgagctgg atccggcacg cccgtacggc attggcgtcg
      781 agctcgtcga agcccatttc accgaggtcg cggcgctgga gcggaccctt gccgtgctcg
      841 gcgcccaact catcgatcgc gcggccgctc ggcccggtgg cgccgcgcgg ctggccgaga
      901 tcatgggtgc tgtggcggct ggctacgcgc aggggctgca agaccgcacc cgcaccgaac
      961 aggagcggat caccgcatcg gcgttcgcgg ccagggtctc ggccgaggaa gcgcggtgga
     1021 acagcgaggc tcgactcgaa gcggtattcg ctgagtcggt cattgggatc gtcatctcgg
     1081 agatcgacgg gaggattctg gaggtcaacc gggccctctg cgagatgctc ggctacaccg
     1141 ccgtggagat caccgggcgt actttctggg agttcgttca cccggacgac gctccgggtt
     1201 tcggggagca cgtcgaggac ctggtggccg gtacgacgaa ccatctccgg gtggagaagc
     1261 tctactctcg caaggacggc gcggccatct ggaccgatct ggtgttgtcg ctgatccggg
     1321 acccggacga gaaaccccgt tacgtcgtcg cgatgatcga gaacatcacc gagcgctatc
     1381 acctgcagac cgacctgcag catcaggcgc agcacgatcc gctcaccggg ctgcccaatc
     1441 ggacggtgtt ctttcaacgc ctggacgccg cgctgctcac cggcgagcag gtgggtatct
     1501 gttacctgga cctcgacgga ttcaagatca tcaacgacac cctcggtcat gatcagggag
     1561 atgagatgct gcgggcggtc gcacaccggt tgatgacgga cctgggccgt gaaggccacc
     1621 tcgtggcgcg gatgggcggc gacgagttcg tggtgctcgt cgagcatggg gcgcaagggg
     1681 agcgattgca gcgcgtcgcg cagatcgcgc tggattcgat ccggcgcccg accctgctgg
     1741 gcggtcacga tgtggttgtc tccgccagtg tcgggatcgt gcagagcggc gacggtggcg
     1801 tcggagcggc ccagctgatg aaggccgcgg atacgacgct ctattgggcc aaggccgacg
     1861 gcggggaccg gtacgtgctg ttcgacgccg agcgccaccg cgccgacgtc gcccggttcg
     1921 cactgtcggc ccggatcccg gaggcgctgg ccgacgggga gttcgtggtc gagtaccagc
     1981 ccctggtccg gctgcgcgat cggcagacga tcggcgtcga ggccctggtg cgctggaagc
     2041 tgccgacggg tgagcagctc cctcccggcg
//
LOCUS       DATCCH010000389         3076 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_439987, whole genome shotgun sequence.
ACCESSION   DATCCH010000389 DATCCH010000000
VERSION     DATCCH010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439987"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="VIJ44_07485"
     CDS             complement(<1..360)
                     /locus_tag="VIJ44_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010592210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEY5165775.1"
                     /translation="MNATQGSLLGFLHDGPMTGWDLLQAVEGGLSRFWNITPSHVYRE
                     LRVLEDRQLVAAGEPGVRDRVPFSLTSAGRATFAAWIHQPPGTEQIRFPLLLTLWFGR
                     HLEPATLAEFVDESREEH"
     gene            433..1191
                     /locus_tag="VIJ44_07490"
     CDS             433..1191
                     /locus_tag="VIJ44_07490"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEY5165776.1"
                     /translation="MVEDVIAELGERGFATIPHAIADDEVASARAALETLVAGTPSGR
                     DDFEGRRTRRVYALFAKTRALDALAVHPDVLGVLDQVLGHYQLSAPAGISIGPDEVCQ
                     PLHPDDAIYPLPRPHPEVVVNVMWPLQDFTKSNGATRIIPGSHRWGDERPGPTAETVT
                     VEMAAGTALLYSGSVWHGGGANETDVDRLGVVLHYAASWLRPVENHVLAVPPAMARTL
                     PVRLQELLGYNIHPPFIGYVDGRHPRRLLQDTER"
     gene            complement(1357..2541)
                     /locus_tag="VIJ44_07495"
     CDS             complement(1357..2541)
                     /locus_tag="VIJ44_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165777.1"
                     /translation="MPRDDYRGDFDPHFELSHLSRQGLAVLGREYMLSGQLQDRSAMP
                     MVLGRLGVEARIGIAIDEWMSASPVYSARMQRALRFSGTDIGTLFKNLQLDIGFSHSY
                     MHVGYRLENEGYGEFWLDCCGALMDVEPFGEDMVRGMCHDIEDPTFDATAAATNPLIK
                     IRPIHRPPRSPADREPHCRWKVFIDDAPNAEPFEMHPNLAIVASSKLADVALVDPGDD
                     AEPGGRADYTDDFDPDFTLEDLSHRALVLTVQEFAVQCHVLCRSFLLTMAQRLPEDEL
                     RTVASAQWTGIAGLTPNGSAPRCGSTATMPARSPSCSSSTRASIPAPTSTPTSRSSTT
                     SASSCASVHLLHSRRAIPCPGSPTSTTTRIPRSMPSPASGTLGPEPIRCRETVRTGPG
                     RS"
     gene            2727..2855
                     /locus_tag="VIJ44_07500"
     CDS             2727..2855
                     /locus_tag="VIJ44_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165778.1"
                     /translation="MALRFGWGGLAGPPFPVWSIDYLIGAAGIFLTYVGFIDRRSA"
     gene            2866..>3076
                     /locus_tag="VIJ44_07505"
     CDS             2866..>3076
                     /locus_tag="VIJ44_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007568140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEY5165779.1"
                     /translation="MRRVVAVLAARSLTFAPVVLTRASPAQARGQRMRLTSPAFATGA
                     MIPIGFTCDDQDVPPPLRWSGLPALT"
BASE COUNT          446 a         1037 c         1092 g          501 t
ORIGIN      
        1 gtgttcctct cggctctcgt ccacgaactc ggcgagggtc gcgggctcga gatggcgccc
       61 gaaccaaagc gtgagcaaga gcgggaagcg gatctgctcg gtgccgggcg gctgatggat
      121 ccacgccgcg aacgtggcgc gaccggcgga ggtcagcgag aagggaaccc ggtcgcgcac
      181 gccgggctcc ccggcggcga ccagctggcg gtcttcgagg acccggagtt ctcggtagac
      241 gtgacttggg gtgatgttcc agaaccggga gaggccccct tcgaccgctt ggagcagatc
      301 ccatcccgtc atcggcccgt cgtggaggaa gcccagtagc gagccctgcg tggcgttcaa
      361 gtcggccacc ttgacattcc cttctggagg gtggacagtg tccaggatat tcccttaggg
      421 agggacgagc cgatggtaga ggacgtgatc gccgagctcg gggagcgcgg cttcgccacg
      481 attccgcatg ccatcgccga cgacgaggtg gcgagtgccc gcgccgcgct cgagacgctc
      541 gtggccggca caccgtcggg gcgcgatgac ttcgagggcc gtcggactcg ccgcgtctac
      601 gccctctttg ccaagacccg ggccctcgac gcgctcgccg tgcacccgga tgtcctcggg
      661 gtcctcgacc aggtcctcgg tcactaccag ctgagcgccc cggcggggat ctcgatcggc
      721 cccgacgagg tctgccagcc gctgcacccc gatgacgcca tctacccgct cccgcgcccg
      781 catcccgagg tcgtggtcaa cgtgatgtgg ccgctccagg acttcaccaa gtcgaacgga
      841 gccacccgga tcatccccgg cagccaccgc tggggcgacg agcggccggg gcccaccgcc
      901 gagacggtca cggtcgagat ggcggcgggc accgcgctgc tgtactccgg gagcgtgtgg
      961 cacggcggcg gtgccaacga gaccgacgtc gaccgactcg gggttgtcct gcactacgcc
     1021 gcgtcgtggc ttcgcccggt cgagaaccat gtcctcgcgg tcccgcccgc aatggcgcgc
     1081 acccttccgg tacgcctcca agagctgttg ggctacaaca tccatccccc gttcatcggc
     1141 tacgtcgacg ggcgacaccc ccggcgcctc ttgcaggaca cggagcgctg acccggcggg
     1201 gcgcggggtg tcgtcgggct gtccgcaccc gatcgttcac gccaccgcgc cgttagctcg
     1261 tctttcgccg gggggcgttc accgcggttg agcccgtgct cagccgggtc atggcgacat
     1321 ccgccggttc cggcgagggc tcggcctgcg gatcgatcac gatctcccag gcccggtgcg
     1381 caccgtttcg cggcaccgga tgggctcggg cccgagggtt cccgacgcgg gcgatggcat
     1441 cgatcgcggg atacgagtcg tcgtcgaggt gggcgagcca ggacagggaa tcgctctcct
     1501 cgaatgcagg agatggaccg aggcgcagga ggacgcgctg gtcgtcgagg acctcgacgt
     1561 cggtgtcgac gtaggtgcgg ggatggaagc tcgggtggag ctggaacagc ttggcgatcg
     1621 cgccggcatc gtcgccgtcg acccgcatcg cggcgcggag ccgttcggcg tcaacccggc
     1681 gatgccggtc cactgggcgc tggccacggt tcggagctcg tcctccggga gacgctgggc
     1741 catggtgagc agaaacgacc gacacagcac gtggcactgg accgcgaact cctgcaccgt
     1801 gaggaccagc gcccggtgcg agaggtcttc gagcgtaaag tcggggtcga agtcgtcggt
     1861 gtagtcggcc cgtccgccgg gttcggcgtc gtccccggga tcgacgagcg cgacgtccgc
     1921 gagcttcgag cttgcgacga tcgcgaggtt cgggtgcatc tcgaacggct cggcgttcgg
     1981 tgcgtcgtcg atgaagacct tccagcgaca gtggggctct cgatcggccg gtgagcgcgg
     2041 gggccggtgg atgggacgga tcttgatgag cgggttcgtc gcggcggcgg tggcgtcgaa
     2101 ggtggggtcc tcgatgtcgt ggcacatgcc ccgcaccatg tcctcgccga acggttcgac
     2161 gtccatgagc gcgccgcagc agtcgagcca gaactcgccg tagccctcgt tctcgaggcg
     2221 gtagccgacg tgcatgtacg agtgtgagaa cccgatatcg agctggaggt tcttgaacag
     2281 cgtcccgatg tcggttcccg agaaccgcag cgctcgttgc atccgtgccg agtagaccgg
     2341 gctggcgctc atccactcat cgatcgcgat cccgatccgc gcttcgacgc cgagtcggcc
     2401 gaggaccatc ggcatcgcgg agcggtcctg cagctggccg ctcagcatgt actcgcgccc
     2461 cagaacggcg agcccctggc gggagaggtg gctcaactcg aagtgggggt cgaagtcgcc
     2521 ccggtagtcg tcgcgtggca cgcgatggaa ccctacgcgg ccgaccggtg cgggtggctc
     2581 gtcgatgagt agcggctact caggacagcc gagccggctc gtgggacgct tccggcatgg
     2641 cctcgacacc accgactccg gttcgtccct ggtacttgcg tccgagggtc gcgatctggc
     2701 tcggaccact cctcatcgtc gcctgcgtgg ccctacggtt cggatggggc ggtctggccg
     2761 ggccaccatt cccagtttgg agcatcgact atctgatcgg cgcggcgggg atcttcctca
     2821 cctacgtcgg cttcatcgac cgccgcagcg cctaacctgc gcgccgtgcg gcgcgtcgtg
     2881 gcggtgctgg ccgcacgcag cctcacgttc gcgccggtcg tgctcacccg cgcgtcgccg
     2941 gcgcaggccc gcgggcaacg gatgcggctc accagcccgg cgttcgcgac gggggcgatg
     3001 atcccgatcg ggttcacctg cgacgaccag gatgtccctc cgccgttgcg ctggtcgggt
     3061 cttccggctc tgaccg
//
LOCUS       DATCCH010000390         5056 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_606545, whole genome shotgun sequence.
ACCESSION   DATCCH010000390 DATCCH010000000
VERSION     DATCCH010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5056
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606545"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="VIJ44_07510"
     CDS             complement(<1..453)
                     /locus_tag="VIJ44_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165780.1"
                     /translation="MTVVDADQHLFEPRTMWGDYGDPTDRDSMLAIVDDEAGNAWLQW
                     QGQRIGLAHVTTPGRPDDVGDQQRRARAGEPPRTRYDEALPDDFWEPTARLARLDQLG
                     VDAAVCFPNYGLFWERELAGDLPATLANMRAWNRWAVQAAHDGRGRLHP"
     gene            509..949
                     /locus_tag="VIJ44_07515"
     CDS             509..949
                     /locus_tag="VIJ44_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5165781.1"
                     /translation="MAVFNHLGQCVADLPRSRRFYEEVLGFEFWREIEPPDSPSDQLL
                     RLTAPIGMTACYLRRDGLVLELLHFAGAAAQPGPARSRGMNELGLTHLSVSVDDLDAT
                     CRAVQAHGGDVLNETNIGAAVFVRDPDGQLIELLPMSYRASVEP"
     gene            946..2493
                     /locus_tag="VIJ44_07520"
     CDS             946..2493
                     /locus_tag="VIJ44_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011600124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY5165782.1"
                     /translation="MTSELVHALTLGDLVRDHARSRPRQLAAIDGDVRLDYRTLDTRV
                     NQLADQLARAGVESGERICWLGQNSFRVLETLLAAAKLGALFCPANWRQSPEELAFVI
                     DDLDPRVVIWQEQEVGPAIEEARRQVTSSPRWVRHDDDTPDSYEAWRDAGSPEDRDRQ
                     VSPEAPLLIVYTAAFGGRPNGAMLSHTACVSQGLVFGHFTATSPSDVYLNSGPLFHLG
                     TLMYLLATFVAGGANVFVPRVDASLLCAVIERERCTGAFVVGPMLDQILAANGDGRYD
                     LSSLRTPRGRPEWNAMTSPDPSPWAAHPGGYGQTEAVGMMTFNCLGVNADGTHGRPSP
                     LIQIRVVDDTDREVDTGEVGEIVARGPTIMCGYWNRPVENVRRHRGGWHHTHDLGRRE
                     ADGSLTFIGPKTRMIKSAAENIYPTEVERCLATHPAVAECAVIGVPDPRWVQSVKAIV
                     VLRDSQTVTEHELMEHCRTQIAPYKKPRSVEFVVALPRDGLVVDYDALDARFGGGNYP
                     GGSTRSA"
     gene            2490..3248
                     /locus_tag="VIJ44_07525"
     CDS             2490..3248
                     /locus_tag="VIJ44_07525"
                     /inference="COORDINATES: protein motif:HMM:NF022586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HEY5165783.1"
                     /translation="MSGPVRSGAYRRRIRVVIDAPGSVAGGLEDDFHYFQVQVRHDAE
                     HVTAVDAQSRRWPWTTCPGAAQPLRALAGMPLSCRCLAVGDWTDPRQNCTHMFDLAGL
                     AVAHAARGGAIGTTRQYDVAIPFGAQGGRRTDVQLWRDGESTLTWTLEGFTCIDPPPY
                     AEVRWRGGFLRWADETFDPDAAEAAIVLRRACDIGMGRGMDLDAIPNAVDILDTQGRI
                     CHTMQPGIVEVAQRNRGTIRDYDGRVEELLADGP"
     gene            complement(3251..4096)
                     /locus_tag="VIJ44_07530"
     CDS             complement(3251..4096)
                     /locus_tag="VIJ44_07530"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEY5165784.1"
                     /translation="MKFGIALGRLNPAFFDVCSLEADRLGYESVWLPEHLVFTTRMSR
                     SPHPGEEHPPVPPTTPIFDALAYLSYLAGRTERVRLGTHVYNVGLRHPFVAARAVQTL
                     DVVSGGRAEFGVGASWLEEEWDAAELDFHTRGRRVDEALAVCRRLWTEAEVSHHGEFF
                     SFDGVVFEPKPPQRPGPPILVGGESDAALRRAARLGDGWIGMSHDFDSGARQITKLRT
                     LLADEGRDSDGFQFCLGGPVDSIADADRWEEIGATRLIVAPWARSKEAVEGMRRFAAE
                     MCLGP"
     gene            complement(4125..4910)
                     /locus_tag="VIJ44_07535"
     CDS             complement(4125..4910)
                     /locus_tag="VIJ44_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020554081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5165785.1"
                     /translation="MPLDPSAALLTDRVAVVTGGAIGIGEAIALAFAQFGAHVAICDR
                     DVENLDGTADGIEAAGRRVVAAELDVRDEEGCRDFVHTVADKLGAVDVLVNNAGGGFF
                     ADFVEVNAKGQDALVRENFASVTNFVRPVVEAMGERGGSIVNLTSIEAHRAAPGYAVY
                     SAMKAAVTSLTMSLALELGPRLIRVNCIAPDVIPTPGVGPMGVHTPLPVAGHVDDVAA
                     CAVFLASDLSRFVTGTTIHVDGGNLAAGGWHRRSDGQYVTHAP"
BASE COUNT          805 a         1775 c         1724 g          752 t
ORIGIN      
        1 gggatgcagg cggccccgcc cgtcgtgggc cgcctggacc gcccaacggt tccaagcccg
       61 catgttggcg agcgtcgcgg ggaggtcacc ggcgagctcc cgctcccaga agagcccgta
      121 gttcgggaag cacaccgcgg cgtcgacacc gagctggtcg agtcgggcca gtcgggccgt
      181 cggctcccag aagtcgtccg ggagcgcctc gtcgtagcgc gtgcggggcg gctcccccgc
      241 ccgggcgcgc cgctgctggt cgcccacgtc gtcgggccga ccgggcgtcg tgacgtgcgc
      301 caacccgatc cgctgcccct gccactggag ccatgcgttg ccggcttcgt cgtcgacgat
      361 cgccaacatc gagtcccggt cggtggggtc gccgtagtcc ccccacatgg tgcggggctc
      421 gaagagatgc tgatcggcgt cgacgaccgt catcgctcta tcgtgccacc cgctcacggg
      481 cttgtcaggg ccgtgggaac atgcgctcat ggcggtcttc aatcatcttg ggcagtgcgt
      541 cgctgacctg ccgcggtcgc ggcggttcta cgaggaagtg ctcggcttcg agttctggcg
      601 tgagatcgag ccgccggact cgccgtctga tcagctgctg cgcctcacag caccgatcgg
      661 gatgaccgcc tgctacctac gacgagacgg gctggtgctc gagctgctgc acttcgccgg
      721 ggccgcggcc cagccggggc cggctcggtc gcggggcatg aacgagctcg gcctgaccca
      781 cctctcggta tcggtcgacg acctcgatgc cacctgtcgc gcggtgcagg cgcacggcgg
      841 cgacgtgctc aacgaaacga acatcggggc cgcggtgttc gtccgtgacc cggatggtca
      901 gctcatcgag ctgttgccaa tgagctaccg ggcgtcggtg gagccttgac ctcggagctc
      961 gtgcacgccc tcaccctcgg cgacctcgtc cgagaccatg cccggagccg gccccgccag
     1021 ctcgccgcca tcgacggtga cgttcgactc gactaccgga cgctcgacac tcgggtgaac
     1081 cagctcgccg atcagctcgc ccgcgccggc gtcgagagcg gcgaacggat ctgctggctc
     1141 ggacagaact cgttccgggt gctcgagacg ctcctcgcgg ccgcgaagct cggggccctg
     1201 ttctgtcccg ccaactggcg tcagagcccc gaggagctgg cgttcgtcat cgacgatctc
     1261 gatcccaggg tcgtgatctg gcaagaacag gaggtcggtc cggccatcga ggaggcccgc
     1321 cgtcaggtga ccagctcacc ccgatgggtt cgccacgacg acgacacgcc cgacagctat
     1381 gaggcgtggc gcgacgcggg ctcccccgag gaccgtgacc gtcaggtcag ccccgaagcc
     1441 ccgctcctga tcgtgtacac cgcggcgttc ggtggtcggc cgaacggggc gatgctcagc
     1501 cacaccgcct gcgtcagcca aggcctcgtg ttcggccact tcacggccac gtcgccctcg
     1561 gacgtgtacc tcaacagcgg ccccctcttt cacctcggga cgctcatgta cctcctggcc
     1621 acgttcgtgg ccggcggcgc caacgtgttc gtgccccgtg tcgacgcgtc gctgctctgc
     1681 gcggtgatcg agcgggagcg gtgcaccggg gccttcgtcg tcggccccat gctcgaccag
     1741 atcctcgccg ccaacggtga cggtcgctac gacctctcga gtctgcggac cccacggggc
     1801 cgacccgaat ggaatgcgat gaccagcccc gatccgagcc catgggcggc gcacccgggt
     1861 ggctacgggc agaccgaagc ggtcggcatg atgacgttca actgtctcgg cgtgaacgcc
     1921 gacggaaccc acggccggcc gtcgcccctc atccagatcc gagtggtcga cgacaccgac
     1981 cgggaggtcg acaccggtga ggtcggcgag atcgtcgccc gcggacccac gatcatgtgc
     2041 ggctactgga accgcccggt ggagaacgtc cgacgccacc gcggcggctg gcatcacacc
     2101 catgacctcg gccgacgaga agccgacggc tccctcacgt tcatcggacc caagacccgc
     2161 atgatcaagt ccgcggccga gaacatctac ccgaccgagg tcgaacgttg cctcgcgacc
     2221 caccctgcag tcgccgagtg tgctgtgatc ggggtccccg atccccgatg ggtgcagagt
     2281 gtcaaggcca tcgtcgtgct gcgtgactcc cagaccgtga ccgaacacga gctgatggag
     2341 cactgccgga cgcagatcgc gccctacaag aagccacggt cggtcgagtt cgtcgtggcg
     2401 ctcccgcgag acggactagt cgttgactat gacgccctcg atgcgcgctt cggtgggggg
     2461 aactatcccg gcggaagcac ccgcagcgca tgagcggacc cgtgcgcagc ggcgcctacc
     2521 ggcgacggat ccgggtcgtg atcgacgcgc cgggctcggt cgccgggggt ctcgaggatg
     2581 acttccacta cttccaggtc caggtccgcc acgacgcgga gcacgtcacc gcggtcgacg
     2641 cccagtcgag gcgctggccc tggaccacgt gtcccggcgc ggcccaaccc ctacgagccc
     2701 tcgcgggtat gcccctgtcc tgccgatgcc tcgcggttgg ggattggacc gaccctcgac
     2761 agaactgcac ccacatgttc gacctcgcgg ggctcgcggt cgcgcacgca gcccggggcg
     2821 gggcgatcgg cacgacccgc cagtacgacg tggcgatccc gttcggggcc caaggtggcc
     2881 ggcgtaccga cgtccagctg tggcgtgacg gcgaatcgac cctgacctgg acccttgaag
     2941 ggtttacgtg catcgatccg cccccatacg cggaggtgcg ttggcgaggc gggttccttc
     3001 gatgggccga cgagaccttc gaccccgacg ccgcggaggc ggcgatcgtg ttgcggcgag
     3061 cctgtgacat cgggatgggc cgtgggatgg acctcgacgc gatcccgaac gcggtcgaca
     3121 tcctcgacac ccagggccgc atctgccaca ccatgcagcc gggcatcgtc gaggtcgcgc
     3181 agcgcaaccg agggacgatc cgcgactacg acggccgcgt cgaggagctg ctcgccgacg
     3241 gtccctaacg tcaggggccc aggcacatct cggctgcgaa gcgccgcatc ccctcgaccg
     3301 cttctttcga tcgggcccag ggggccacga tgaggcgagt cgcgccgatc tcctcccagc
     3361 ggtcggcgtc ggcgatcgag tcgaccggac cgccgagaca gaattggaac ccgtccgagt
     3421 cacgcccctc gtcagcgagc agtgtccgga gcttggtgat ctggcgagca ccggagtcga
     3481 agtcgtggct catgccgatc cagccgtcac ccagccgggc ggcgcgacgt agcgcggcgt
     3541 cgctctcccc tcccaccagg atgggaggcc cgggacgctg gggcggtttg ggttcgaaca
     3601 ccaccccgtc gaaggagaag aactcgccgt ggtgggagac ctccgcttcg gtccatagcc
     3661 gccgacagac cgcgagggcc tcgtcgaccc gacgaccgcg ggtatggaag tcgagctccg
     3721 cggcgtccca ctcctcctcg agccagctgg cgccgacacc gaactccgcc cgtccaccgg
     3781 agacgacgtc gagggtctgc accgcccggg cggcgacgaa gggatggcgg agaccgacgt
     3841 tgtacacgtg ggttccgaga cggacccgct cggtacgacc agcaaggtac gagaggtagg
     3901 ccaacgcgtc gaagatcggc gtggtgggcg gaacaggagg atgctcctca cccggatgcg
     3961 gtgagcggct catccgggtg gtgaagacga ggtgctcggg gagccagacc gactcgtagc
     4021 cgagccggtc ggcttcgagg gagcagacgt cgaagaacgc cgggttgaga cggccgagcg
     4081 cgatcccgaa cttcaccgcg agctccgacg ccagatcagg atcgtcatgg ggcgtgcgtg
     4141 acgtactggc cgtcggagcg gcggtgccaa ccgccggcgg cgaggttgcc gccgtcgacg
     4201 tggatggtgg tcccggtaac gaaccggctg aggtcgctcg ccaggaacac cgcacacgca
     4261 gcgacatcgt cgacatgtcc ggcgaccggg aggggcgtgt gaacacccat cggcccgacg
     4321 cccggggtgg gaatcacgtc gggggcgatg cagttcacgc ggatcaggcg aggaccgagt
     4381 tccaaggcga ggctcatcgt caggctcgtg accgccgcct tcatcgccga gtacaccgcg
     4441 taccccggcg ccgcgcgatg cgcctcgatg gaagtgaggt tcacgatcga cccgccccgc
     4501 tcccccatcg cctcgacgac cggtcggacg aagttcgtga cgctggcgaa gttctcccgc
     4561 accaacgcgt cttggccctt ggcgttgacc tccacgaagt cggcgaagaa cccgccgccg
     4621 gcattgttga cgagcacgtc gaccgcccca agcttgtcgg ccaccgtgtg cacgaagtcg
     4681 cgacagccct cctcgtcccg gacatcgagc tcggccgcga cgacccgccg cccggcagct
     4741 tcgatcccgt ccgcggtgcc gtcgaggttc tcgacgtctc ggtcgcagat cgcgacatgg
     4801 gcaccgaatt gggcgaacgc caaggcgatc gcctcgccga tcccgatcgc gcctccggtg
     4861 acgaccgcga cacggtccgt gagcagagcc gcggacgggt caaggggcac ggcgccggac
     4921 agtacccctc gttaaccgtt agcgtcgggc aggtgagcta ctcgatcgaa ggcaagcacg
     4981 tgctcgtgac gggcgcctcg tcagggatcg gtgccgcact cgcaacgggc ttcgcccggc
     5041 gaggtgcggt ggtcgg
//
LOCUS       DATCCH010000391         2054 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_606612, whole genome shotgun sequence.
ACCESSION   DATCCH010000391 DATCCH010000000
VERSION     DATCCH010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606612"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /locus_tag="VIJ44_07540"
     CDS             <1..862
                     /locus_tag="VIJ44_07540"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY5165786.1"
                     /translation="HQFDAAETLELIDRYQVTNVHLVPTQLTRLLRLPDATRAPFSGA
                     SLRVVYHGAAPCPPDVKRRMIDWWGPKLVEYYGGTEGAMLSTIDSPDWLDHPTSVGKP
                     WPTVQVLVLHDDGSPCAVGEEGTLYVRNLLGMDFEYHGDEDKTAAAHREPGVFTLGDI
                     GYLDTDGYLHLSDRRIDLIISGGVNIYPAEIEGVMQAHPAVRDVAVFGIPNDEFGEEV
                     KAAVELEPGHEASSALVTELLGYCRASLAGYKVPRSIDFEAVLPRHPTGKLYKRVLRD
                     AYWQDTGRRI"
     gene            complement(832..1116)
                     /locus_tag="VIJ44_07545"
     CDS             complement(832..1116)
                     /locus_tag="VIJ44_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165787.1"
                     /translation="MIAAACLGAGALVIAALGLVLAAVDAGLVASIAEGLPRTGTAIL
                     AFEGVTLALVMTQLARPAAVAVLAATGAAWFWAHHRAGGVRQIRRPVSCQ"
     gene            complement(1113..1262)
                     /locus_tag="VIJ44_07550"
     CDS             complement(1113..1262)
                     /locus_tag="VIJ44_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165788.1"
                     /translation="MALSSPPGTIRGPGPEDEMRDQDQSHPTIVVADAHRAAVLRNVE
                     ALGSP"
     gene            1378..1992
                     /locus_tag="VIJ44_07555"
     CDS             1378..1992
                     /locus_tag="VIJ44_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165789.1"
                     /translation="MAAGLDLGAVGPAIQGVVEQALDTVVARAVAEPLPVYSPDDVRA
                     ELEGPSAFTVTTLVAPALTGVAKRFSNRVVRLGSKASFSVKALLAALPPITSSVTLGA
                     RELHVLASFVVNRLGAADVPVDRRFVQRVTVNAYVWPRGGRELEAPQAVAVLRVAGLW
                     ATRPLARERNGEWVGRAADAINDADLSDRYERYRREREDLSPGQ"
BASE COUNT          293 a          717 c          741 g          303 t
ORIGIN      
        1 ccaccaattc gacgccgccg agaccctgga gctgatcgac cgctaccaag tgaccaacgt
       61 ccatcttgtg ccgacacagc tgacgcgctt gctgcgcctc cccgacgcga cccgcgcccc
      121 gttctccggt gcgagtctcc gcgtcgtcta ccacggtgcc gcgccgtgcc cgccggatgt
      181 gaagcggcgc atgatcgact ggtggggccc gaagctggtc gagtactacg gcggaaccga
      241 aggggcgatg ctgtcgacga tcgactcgcc cgactggctc gaccatccga ccagtgtcgg
      301 gaagccctgg ccgacggtgc aggtcctcgt cctccacgac gacggcagcc cctgcgcggt
      361 cggcgaggaa ggcacgctgt atgtccgcaa cctcctcggc atggacttcg agtaccacgg
      421 cgacgaggac aagaccgccg cagcgcaccg agagccgggc gtgttcaccc tcggcgacat
      481 cggctacctc gacaccgacg gctacctgca tctctccgat cggcggatcg acctcatcat
      541 ctcgggtggg gtgaacatct acccggcgga gatcgaaggg gtcatgcagg ctcaccccgc
      601 ggtgcgcgac gtggcggtgt tcgggatccc gaacgacgag ttcggtgagg aggtcaaggc
      661 cgcggtcgag ctcgaacccg gacacgaagc gtcatcggcg ctcgtgacgg aactcctcgg
      721 gtactgccgg gccagcctcg cgggctacaa ggtgccgcgc agcatcgact tcgaagccgt
      781 gcttcctcgt cacccgaccg gcaagctcta caagcgggtg ctgcgcgacg cctactggca
      841 ggacaccggg cgtcgaattt gacggacgcc gccggcacgg tggtgcgccc agaaccaggc
      901 ggcgccggtc gcagcgagca ccgcgacggc cgcgggtcgc gccagttgcg tcatcaccaa
      961 cgccagggtg acaccttcga aggcgaggat cgcggtgccg gtgcggggga gcccttcggc
     1021 gatcgaggcg accaggccgg cgtcgaccgc ggcgaggacg aggcccagcg ccgcgatcac
     1081 gagcgcgccc gccccgaggc acgccgcggc gatcacggcg agccgagcgc ctcgacgttg
     1141 cggaggaccg cggcgcggtg cgcgtccgcc accacgatgg tcgggtggct ctgatcctgg
     1201 tcgcgcatct cgtcctccgg gcccggcccc cggatcgtcc cgggtgggga tgagagggcc
     1261 atgagggctc ggtcagcggc ccgcccacgt tcgggccgcc cctaaggtcg ggggcatcag
     1321 cctggtccac cttttcctca gcgtgccgat cacctcggcg gcccggggtt cgcgttgatg
     1381 gccgccggcc tcgatctcgg ggccgttggc ccggcgatcc aaggcgtcgt cgagcaggcg
     1441 ctcgacaccg tcgtcgcccg ggcggtcgcg gagccgttgc cggtgtactc gccggacgac
     1501 gtgcgggccg aactcgaagg gccgtcggcg ttcaccgtga cgacgttggt ggcgccggcg
     1561 ttgacgggcg tggccaagcg gttctccaat cgggtggtgc ggctcggctc caaggcgtcg
     1621 ttctcggtca aggccctgct cgccgcgctg ccgccgatca cgtcgtcggt gacgctcggg
     1681 gctcgagagc tccacgtgct cgcgtcgttc gtcgtcaacc ggctaggtgc tgccgatgtc
     1741 ccggtcgatc gccggttcgt gcagcgggtc acggtcaacg cgtacgtctg gccccgcggc
     1801 ggccgcgagc tggaagcccc gcaggcggtc gcggtgttgc gagtcgcggg cctgtgggcg
     1861 acccgaccgc tggctcgtga gcgcaacggt gagtgggtgg ggcgcgccgc ggacgcgatc
     1921 aacgacgccg acctttccga ccggtacgag cgctaccggc gcgagcgaga agacctgtcc
     1981 cccggtcagt gaccggggcc gctatcggcc gagggcctcc acggtgcggt cgagctcggc
     2041 gggatcggcg gtgg
//
LOCUS       DATCCH010000392         4413 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_606736, whole genome shotgun sequence.
ACCESSION   DATCCH010000392 DATCCH010000000
VERSION     DATCCH010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4413
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606736"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..648
                     /locus_tag="VIJ44_07560"
     CDS             109..648
                     /locus_tag="VIJ44_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEY5165790.1"
                     /translation="MRLGPEPVRSFLWRRHRDVRRLGRPLMRSGSLDAIFATGVGGAP
                     MRSLEAVEAVAGEGLRGDRYSEHRGHWSPLDECQVTLVAAETLDEIETTFGVSVHAGQ
                     HRRNLVTRGLDLMNLYGRTFTIGDAVLAFDRPRPPCRYIASITEPGMTRALGARRGGI
                     CARVVTSGVVRLGDDIVIR"
     gene            complement(650..1579)
                     /locus_tag="VIJ44_07565"
     CDS             complement(650..1579)
                     /locus_tag="VIJ44_07565"
                     /inference="COORDINATES: protein motif:HMM:NF018447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HEY5165791.1"
                     /translation="MAVQSRLGAGIGRVAGTLLLATLVFGITLPAGAQTGGTDATVQS
                     LRQQAEAASGAYFAALAKSQVVQAQIDSLEAQLPQLRAEEQTRLRTAAHRAVSAYEGS
                     GAQLGIILGSGDLLTAARRAHWLEQLNAQDNRELANLRRAADRLTGQERALRAAQQAQ
                     AATLQDLQARGHDIDVKLQAAEARQRALDAAAAVAAQQAAPAGGGAPAGGSPGAGYSP
                     TPGSHPHHNDPFLICTRDRESGGNYGAFNPSGPYMGAYQFLQSTWDSAANHAGRPALI
                     GVQPNTASVYDQDDMAWNLYQWQGTGPWGGRCG"
     gene            1752..2459
                     /gene="aat"
                     /locus_tag="VIJ44_07570"
     CDS             1752..2459
                     /gene="aat"
                     /locus_tag="VIJ44_07570"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEY5165792.1"
                     /translation="MREGGPLTGAWGGPAPTGPTRWSLPDPREAAEELVGLGADLEPA
                     TLVAAYRQGLFPMRLEQDGPLGWWSPNPRGVIPLRGLHTARSLRRSARRFEVTVDTAF
                     MPVVRGCADPGRPHGWIDEEFMAAYSRLHALGWAHSIEVWGPRGTDVPMLAGGLYGVA
                     IGGLFAGESMFHRARDASKVALVALIDLLQKGGGVLLDVQWCTPHLATLGAVEMARSR
                     YLDLLEDAVNRPQLRFG"
     gene            complement(2469..3602)
                     /locus_tag="VIJ44_07575"
     CDS             complement(2469..3602)
                     /locus_tag="VIJ44_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEY5165793.1"
                     /translation="MTTTDAEFKALGRDDVNAVESILADDSIDFGAVAHSTPVGGDAV
                     FTWDYARTRAGLAKLYERAKTSQWNGATDLDWSVEVDVERVSETMQLAMAPLRGRLTD
                     LPGSPLKSWGEKEWTSLGVDAFRWRMSQFLHGEQGALVCTAKIVETVPWIDAKYYAAT
                     QVMDEARHVEVFARYLDTKLDGGFPVNPNLQLLIDDVLGDNRWDMTYMGMQIMIEGLA
                     LAAFGFMYQVTDEPLLKKLLRYVLSDEARHVAFGVLSLKEYYEGLGGREIRERQEFAF
                     EAAIRMRDRLMMQAVWERAGIDRATITRMLFEIRQPGVNPFQASLFAKIVPNCKKLGL
                     LDAGDGWLRDRFTEIGVIDFEDAPDTEVEYELFDAVAADRAAG"
     gene            3652..4023
                     /gene="arsC"
                     /locus_tag="VIJ44_07580"
     CDS             3652..4023
                     /gene="arsC"
                     /locus_tag="VIJ44_07580"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00014.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEY5165794.1"
                     /translation="MGDVLVYHNPRCSKSRGALELLNARGVDPDVVEYLQAPPDRAAL
                     ERIMDAIAEPPSALIRHDDRFRELGLDPNGYQDRATVAALLLAHPELMQRPVIIRGGR
                     GVIARPSERVLELLNPDPGAS"
     gene            4023..>4413
                     /locus_tag="VIJ44_07585"
     CDS             4023..>4413
                     /locus_tag="VIJ44_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY5165795.1"
                     /translation="MATTTDTSVRDELRSWLAGAWDPGVTVGEWWERLADSGWAVPTW
                     PAEWLGRDLPRELAPLVAAELRDAGAIGPPAGLGMLLAGPTILVHGTDEQKRRYLRPI
                     VTGEEGWCQLFSEPGAGSDLASLATRAV"
BASE COUNT          609 a         1532 c         1568 g          704 t
ORIGIN      
        1 gctgcgatcc gatgtccgtc gggatgctgg acgcctggcg caaccagtcc gacgagacgt
       61 tcatgctctc gatgctcccc acctggatcg gcgagggcta ggcccgcaat gcgtctcggc
      121 cccgagccgg tgcggtcgtt cctgtggcgg cgccaccgcg acgttcgtcg cctcggacga
      181 ccgctcatgc ggtcggggag cctcgacgcc atcttcgcca ccggcgtggg cggcgccccg
      241 atgaggtcgc tcgaggcggt ggaggcggtc gccggtgagg ggttgcgggg tgaccggtac
      301 tcagagcatc gggggcattg gagcccgctc gacgagtgtc aggtgaccct ggtcgcggcc
      361 gagaccctcg acgagatcga aaccacgttc ggcgtgtccg tccacgcggg gcagcatcgt
      421 cgcaaccttg tgacccgagg cctcgatctc atgaacctgt acggccgcac tttcacgatc
      481 ggcgacgcgg tgttggcctt cgatcggccc cgcccgccgt gtcgctacat cgcgtcgatt
      541 accgagcccg ggatgacgcg tgcgctgggt gcccgtcgag gcgggatctg cgctcgggtc
      601 gtcacctcgg gtgtggtccg gcttggtgac gacatcgtca tccggtaggt cagccgcagc
      661 ggccgcccca cggcccggtt ccctgccatt ggtacaggtt ccaggccatg tcgtcttggt
      721 cgtacaccga cgccgtgttg ggctgcacac cgatcagcgc gggacgcccc gcgtggttgg
      781 cggcgctgtc ccacgtcgac tgcaggaact ggtaggcccc catgtagggc ccggaggggt
      841 tgaacgcccc gtagttcccg ccgctctccc ggtcgcgggt gcagatgagg aacgggtcgt
      901 tgtgatgggg gtgcgaaccc ggggtcgggc tgtaccccgc gccgggtgac ccgcccgctg
      961 gcgcgccacc gcccgccggc gcggcctgtt gcgcggcgac cgcggcggcg gcatcgagcg
     1021 cgcgttggcg ggcctcggcg gcctgcaact tgacgtcgat gtcatggcct cgggcttgga
     1081 gatcttggag cgtcgcggcc tgggcctgtt gcgcggcgcg taaggcccgt tcctgtccgg
     1141 tgaggcgatc ggcggcccgg cgaaggttgg cgagttcccg gttgtcctgg gcgttgagct
     1201 gttcgagcca gtgggcccgt cgcgccgcgg tcagcaggtc tccgctgccg aggatgatgc
     1261 ccagctgcgc tcccgagcct tcgtacgccg agacggcccg atgcgccgcg gtgcgaagcc
     1321 gggtctgctc ctcggcgcgc agctggggga gttgggcctc gaggctgtcg atctgggcct
     1381 gaaccacctg ggacttggcg agcgccgcga agtaggcgcc cgacgcggcc tcggcctgct
     1441 gacgcaacga ctggactgtg gcgtcggtgc cgccggtctg ggccccggcc gggagcgtga
     1501 tcccgaacac cagggtcgcg agtagcagcg ttccggccac ccggccgatc cccgccccga
     1561 ggcgggactg gacggccaac cggatcctcc tcgggacggg ggctgcgacg ccggcttgtg
     1621 ccgcgtccct agccccggtg gggaacaaca tcacccacgg tagtgcgaga cggcctagcc
     1681 aggtggcgcc caagggcacc ggtacgctga cgccccgcca ttcgatccag gagtctgtcg
     1741 ttgcgagcac cgtgcgcgaa ggaggcccgc tgaccggggc gtggggcggc ccggccccga
     1801 cgggaccgac ccgctggtcg ctgcccgatc cccgagaggc ggccgaggag ctcgtcggcc
     1861 tcggcgccga tctggagccg gccacgctcg tcgctgccta ccggcagggg ctgttcccaa
     1921 tgcgcctgga gcaggacgga cccctcggct ggtggtcacc gaatccccgg ggcgtgatcc
     1981 ccctgcgggg cctgcacacg gcgcggtcgc ttcgccgctc ggcccggcgc ttcgaggtca
     2041 cggtcgacac cgccttcatg cccgtggttc gagggtgcgc cgatcctgga cgaccgcacg
     2101 gctggatcga cgaggagttc atggccgcgt actcgaggct gcacgcgctc ggatgggcgc
     2161 acagcatcga ggtgtggggc ccgcgcggca ccgacgtccc gatgctggcc ggcggcctct
     2221 acggggtcgc gatcgggggc ctcttcgccg gtgaatcgat gttccaccgg gctcgagacg
     2281 cttcgaaggt ggccctcgtc gccctcatcg acctcctcca aaaaggcgga ggggtcctcc
     2341 tggacgtcca gtggtgcacc ccacacctcg ccacgctggg cgcggtcgag atggcccgga
     2401 gccggtacct cgacttgctc gaagacgcgg tgaaccgacc ccagcttcgc ttcggctgag
     2461 acgcgccgtc agcccgccgc ccggtcggct gcgaccgcgt cgaagagctc gtactcgacc
     2521 tcggtatccg gggcgtcctc gaagtcgatc actccgatct cggtgaaccg gtcccgcagc
     2581 cagccgtcgc cggcgtcgag gagcccgagc ttcttgcagt tcgggacgat cttggcgaac
     2641 aacgacgcct ggaacgggtt cacgccgggc tggcggatct cgaagagcat ccgggtgatc
     2701 gtcgcgcggt cgatgccggc ccgttcccac acggcctgca tcatgagccg gtcgcgcata
     2761 cggatcgcgg cctcgaacgc gaactcctga cgctcgcgga tctcgcggcc gccgagccct
     2821 tcgtagtact ccttgagcga cagcacaccg aacgcgacgt ggcgggcctc gtcgctcaag
     2881 acataccgca gaagcttctt cagcagcggc tcgtcggtga cctgatacat gaacccgaac
     2941 gcggcgaggg caagtccctc gatcatgatc tgcatgccca tgtaggtcat gtcccagcgg
     3001 ttgtcgccga gcacgtcgtc gatgagcaac tgcaggttgg ggttcacggg gaagccgccg
     3061 tcgagcttgg tgtcgagata gcgggcgaag acctcgacgt ggcgggcctc gtccatcacc
     3121 tgggtcgcgg cgtagtactt ggcgtcgatc caggggacgg tctcgacgat cttggccgtg
     3181 caaaccagcg ccccttgctc gccgtggagg aactggctca tccgccaccg gaacgcgtcg
     3241 accccgaggc tggtccactc cttctctccc cacgacttga gcggcgagcc cggcagatcg
     3301 gtcaggcggc cccggagcgg ggccatggcg agttgcatcg tctcggagac ccgctcgacg
     3361 tcgacctcca ccgaccagtc gaggtcggtg gcgccgttcc actgggaggt cttggcccgc
     3421 tcgtagagct tggcgagacc ggcccgggtt cgggcgtaat cccatgtgaa cacggcatcg
     3481 ccgcccaccg gcgtcgagtg ggcgaccgca ccgaagtcga tcgagtcgtc ggcgaggatc
     3541 gactcgaccg cgttgacgtc gtcgcgaccc agggccttga actcagcgtc ggtcgtggtc
     3601 atcggggctc cgagggtcgt ggggatcagc accaaacggt accctgcgga tgtgggcgac
     3661 gtgctcgtgt accacaaccc ccggtgttcc aaatcccggg gcgcgttgga gctgctgaac
     3721 gcgcgaggtg tcgacccgga cgtcgtcgag tacctccagg cacccccgga ccgggcggcg
     3781 ctggaacgga tcatggacgc catcgccgag ccgccgtcgg cgttgatccg ccacgacgac
     3841 cggttccggg agctcggcct cgaccccaac gggtatcagg accgggccac cgtggcggcg
     3901 ctgctcctgg cccaccctga gctcatgcag cgacccgtga tcatccgggg tggacgtggt
     3961 gttatcgccc gcccgtccga acgagtcctg gaactcctca accccgaccc gggagcatcc
     4021 tgatggccac gaccaccgac acgtccgtgc gcgacgagct gcgttcctgg ctggccggtg
     4081 cctgggaccc cggtgtgacc gtcggtgaat ggtgggaacg gctggccgac tcggggtggg
     4141 cggtcccgac gtggccggcc gagtggctcg gccgcgacct gccccgcgag ctggctccgc
     4201 tcgtcgccgc ggagctgcgc gacgccgggg ccatcggacc gcccgcaggg ctcggcatgc
     4261 tcctcgccgg ccccaccatc ctcgtccacg ggaccgatga gcagaagcgg cggtacctgc
     4321 gcccgatcgt caccggcgaa gagggatggt gccagctgtt cagcgagccg ggcgccggct
     4381 cggacctggc gagcctcgcc acccgtgcgg tgc
//
LOCUS       DATCCH010000393         3563 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_440253, whole genome shotgun sequence.
ACCESSION   DATCCH010000393 DATCCH010000000
VERSION     DATCCH010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440253"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1607
                     /locus_tag="VIJ44_07590"
     CDS             <1..1607
                     /locus_tag="VIJ44_07590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY5165796.1"
                     /translation="IVIVLDNSILNVALPTIVQTLHASNSQLQWMVDSYTLVFAGLLL
                     TMGSVGDKYGRRPALQAGFLVFGAGSLLSALAGSADQLIASRAFMGIRGALIMPATLS
                     IITNVFPAHERPKAIGVWAATAGVGVALGPLTGGFLLEHFYWGSIFLVNLPIVMFGVI
                     AGIYLIPNSKDPNAPRLDLVGAALSIIGLSALLYAVIEAPTKGWTADSTLASFAIGGL
                     VMAAFFAWERRVDHPMLDMGFWKNPRFSGASGAIAITFFAMFGSIFLLTQYLQFVLGY
                     TPFQTGIRLLAFAIPMMVIAPFSPRLVDRIGTKFTVALGLVCVSVGLVLLGGVGATTG
                     YADIAWRMALAATGMALTMSPATESIMGSLPLTRAGVGSAVNDTTRQVGGAVGVAVIG
                     SVFSSIYGSKVVDALRGKGVPGSVVANAKSSLGAALQTAAHLPGAAGRVFADLAKTAF
                     IDGFHSGVYVGAAASALGIIAVLIWLPARAHADDVERQAADFDAHGHSGPRSTVAAPG
                     NGDGDAVREPPGDGVPVESAAPARTR"
     gene            1604..2221
                     /locus_tag="VIJ44_07595"
     CDS             1604..2221
                     /locus_tag="VIJ44_07595"
                     /inference="COORDINATES: protein motif:HMM:NF028169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY5165797.1"
                     /translation="MNDVLRPGRPRSAEADQAIVDAALAVFAEEGFDGLTMEAVAARA
                     GVGKATVYRRYPGKAELVIRAASCLSAAEAPEADTGSLRGDLQAISRNLVHLLTATVA
                     GRCVPELVAALPHCAQLAGEHRRFIAARRRLTVRAVGRAVARGELVADTDAEIVADLL
                     AGPIFYRHLVVRGCLDNRYADRVVDAVLGWLSHDIPAGAVCVVPA"
     gene            complement(2229..>3563)
                     /gene="kgd"
                     /locus_tag="VIJ44_07600"
     CDS             complement(2229..>3563)
                     /gene="kgd"
                     /locus_tag="VIJ44_07600"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="kgd; produces succinic semialdehyde; part of
                     alternative pathway from alpha-ketoglutarate to succinate;
                     essential for normal growth; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HEY5165798.1"
                     /translation="LVGRGDLTADECDAAFADFRARLDRAFEETHEPHATGIDPDAEP
                     LDVNESAEPADPVATAVDRSVLDRVVAGLTAWPDTFHVHPKLERILVAQRDAYHAGTV
                     EWPLAESLAFGSLVLEGTPVRVAGQDTRRGTFSQRHGVLVDAVDEAEYVPLQHLAPDQ
                     APFRLYDTVLSEYAALGFEYGYSVAAPETFTAWEAQFGDFMNAAQVTIDEFVVAAEDK
                     WGQRSGLTMLLPHGFEGQGPDHSSARIERFLVLCADDNLRVVYPSTAAQYFHVLRRQV
                     RAARRVPLVCFTPKRYLRMQQARSPVELLTRDTFHRVLDDRATDLDPATVRRVLLCTG
                     KLGHELMDARDQAGTPVAVVRLEQLYPWPEPEIRAVLARYPKESQLWWVQEEPANMGA
                     WTFVDGALRRSLGDTRRWDHIARHASASPATGSAKVHEREQQAVVGAAVGGA"
BASE COUNT          482 a         1273 c         1243 g          565 t
ORIGIN      
        1 tcatcgtgat cgtgctcgac aactcgatcc tgaacgtggc cttgccgacc atcgtgcaga
       61 ccctccacgc gtcgaacagt cagctgcagt ggatggtcga ctcctacacg ctggtgttcg
      121 cgggcttgct cctcacgatg ggcagtgtcg gcgacaagta cgggcggcgc cccgcgctcc
      181 aagccgggtt cctcgtgttc ggcgccgggt ccctgctctc ggcgctcgcc ggctcggctg
      241 atcagctcat cgccagccgg gcgttcatgg gcatcagggg cgcgctgatc atgccggcga
      301 cgctgtcgat catcacgaac gtgttcccgg cccacgaacg accgaaggcc atcggcgtgt
      361 gggccgcgac cgccggagtc ggcgtggcgc tcggtcccct caccggcggg ttcctgctgg
      421 agcacttcta ctggggctcg atcttcctcg tgaacctccc catcgtgatg ttcggggtga
      481 tcgcggggat ctacctgatc ccgaactcga aggaccccaa cgcgccccgc ctcgacctcg
      541 tcggcgcggc cctgtcgatc atcgggctca gcgcgctgct ctacgcggtg atcgaggccc
      601 ccacgaaggg atggaccgcc gactcgacgc tcgcgtcctt cgccatcggc gggctggtca
      661 tggccgcgtt cttcgcctgg gagcgtcgcg tcgaccatcc gatgctcgac atgggcttct
      721 ggaagaaccc gcggttcagc ggcgcgagcg gcgcgatcgc gatcacgttc ttcgccatgt
      781 tcggctcgat cttcctcctg acccagtacc tgcagttcgt gctcggctac acgccgttcc
      841 agaccgggat ccggctcctc gccttcgcga tcccgatgat ggtcatcgcc ccgttcagtc
      901 cccggctcgt ggaccggatc ggcaccaagt tcacggtggc gctcggtctg gtgtgcgtct
      961 cggtgggtct ggtcctgctc ggcggcgtcg gcgccacgac gggctacgcc gacatcgcgt
     1021 ggcggatggc gctcgccgcg accgggatgg cgctcaccat gagcccggcc acggaatcga
     1081 tcatgggctc gctcccgctg acccgggccg gtgtcggctc cgcggtcaat gacaccaccc
     1141 gccaggtcgg cggcgcggtc ggcgtcgcgg tcatcggcag cgtcttctcg tcgatctacg
     1201 gctcgaaggt cgtggacgcg ctgcgcggca agggggtgcc gggaagcgtg gtggcgaacg
     1261 ccaagtcgtc cctcggtgcc gcgctccaga ccgcggcgca tctccccggg gccgccggtc
     1321 gcgtcttcgc cgatctcgcc aagaccgcgt tcatcgacgg gttccacagc ggcgtctacg
     1381 tcggtgccgc ggcgtcggcg ctcgggatca tcgcggtgct gatctggctg ccggcgcgcg
     1441 cccacgccga cgacgtcgaa cgtcaagccg ccgactttga cgcgcacggc cactctgggc
     1501 ctcgcagcac ggtggcggcg ccaggaaacg gtgacggtga cgcggtccgc gaacccccgg
     1561 gtgacggcgt ccccgtcgag tcggcggcgc ccgcccggac gcgatgaacg acgtgctgcg
     1621 cccgggtcgg ccccgcagcg ccgaggccga ccaggccatc gtcgacgccg cgctggcggt
     1681 gttcgccgag gagggcttcg acggtctcac catggaggcc gtggccgccc gggccggcgt
     1741 cgggaaggcc acggtgtacc gccgctatcc gggcaaggcc gagctggtca tccgcgccgc
     1801 gtcgtgtctc agcgccgcgg aggcccccga ggccgacacc ggctcgctcc gaggggactt
     1861 gcaggccatt tcccgcaacc tcgtgcacct gcttaccgcg acggtcgccg gacgatgcgt
     1921 ccccgagctc gtcgccgcgt tgccccactg cgcgcagctc gccggcgagc atcgacgctt
     1981 catcgccgcc cgtcgtcgcc tcaccgtccg ggcggtgggc cgggccgttg cccgaggcga
     2041 gctcgtcgcc gacaccgacg ccgagatcgt cgccgacctc ctggccggac cgatcttcta
     2101 tcggcacctc gtcgtgcggg gatgcctcga caaccggtac gccgaccgcg tcgtcgacgc
     2161 cgtcctcggc tggctgagcc acgacatacc ggccggtgcc gtgtgcgtcg tgcccgcctg
     2221 agcgaagctc aagcgccgcc caccgccgcg ccgacgacgg cctgctgttc gcgctcgtgg
     2281 accttcgcgc tcccagtggc ggggctggcg ctggcgtgac gcgcgatgtg atcccaacgt
     2341 cgggtgtcgc cgagtgagcg gcgcaaggca ccgtcgacga aggtccaggc ccccatgttc
     2401 gccggctcct cctggaccca ccacagctgg gactccttcg ggtaccgggc gagcacggcc
     2461 cggatctccg gctcgggcca ggggtagagc tgctcgagac ggaccaccgc caccggagtg
     2521 ccggcctggt cgcgggcgtc catgagctcg tggccgagtt tgccggtgca gagcagcacc
     2581 cgccggacag tcgcgggatc gaggtccgtg gcgcgatcgt cgagcacccg gtggaaggtg
     2641 tcgcgggtga gcagctcgac cggcgaccgg gcctgttgca tccgcaggta ccgcttgggg
     2701 gtgaagcaca ccagcggcac ccgtcgggcg gcgcggacct ggcggcgcag cacatggaag
     2761 tactgcgccg cggtcgacgg gtagacgacg cgcaggttgt cgtcggcgca caggaccagg
     2821 aaccgttcga ttcgggcgct cgagtggtca gggccctggc cttcgaagcc gtgcggcaac
     2881 agcatcgtca gccccgaccg ctggccccac ttgtcctctg cggcgacgac gaactcgtcg
     2941 atggtgacct gcgccgcgtt catgaagtca ccgaactggg cttcccaggc ggtgaacgtc
     3001 tcgggggctg ccaccgagta gccgtactcg aatccgaggg ctgcgtactc ggacaggacc
     3061 gtgtcgtaga ggcgaaacgg cgcctgatcg ggggcgagat gctggagcgg gacgtactcg
     3121 gcttcgtcga cggcgtcgac caggacacca tgacgctggc tgaacgttcc ccgacgggtg
     3181 tcctgaccgg cgactcgcac cggtgtgccc tccaagacga gcgaaccgaa cgccaacgac
     3241 tcggccagcg gccactccac cgtccccgcg tggtacgcgt cgcgctgggc cacgaggatc
     3301 cgctccaact tcgggtggac gtggaacgtg tcgggccagg cggtcaaccc ggcgaccacg
     3361 cgatccagga ccgaccggtc gacggcggtc gcgaccgggt ccgccggctc cgccgactcg
     3421 ttcacgtcga ggggctcggc gtcggggtcg attccggtgg cgtggggctc gtgggtctcc
     3481 tcgaacgccc ggtcgagccg ggcccggaaa tcggcgaacg cggcgtcgca ttcgtcagcg
     3541 gtgaggtccc cgcgaccgac gag
//
LOCUS       DATCCH010000394         2180 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_606930, whole genome shotgun sequence.
ACCESSION   DATCCH010000394 DATCCH010000000
VERSION     DATCCH010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606930"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1560)
                     /locus_tag="VIJ44_07605"
     CDS             complement(<1..1560)
                     /locus_tag="VIJ44_07605"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEY5165799.1"
                     /translation="MAKHIFVTGGVASSLGKGLTAASLGRLLKSRGLRVTLQKLDPYI
                     NVDPGTMNPFQHGEVFVTEDGGETDLDLGHYERFVDVPLTKKSNATTGSIYQEVLAKE
                     RRGAYLGQTVQVIPHITNEIKDRILRLADDDVDVVITEVGGTVGDIEILPFLEAIRQF
                     RKDVGRDNVFYVHVTLVPFIGPSGEQKTKPTQHSVTELRSRGIQPDAIVCRSDRPIPI
                     RLKEKISQLCDVPEEGIVSAIDASNLYEIPLVLHDEGLDNYVCRVLQLDHHEPDLSSW
                     RALVSRIESAEQTVRIGIVGKYVNLPDAYLSVAEAIRHGGYGCGARIELDWIASDDAE
                     GLLAEGRLRELDGIVIPGGFGVRGIEGKIAAAGYAREHQIPYLGLCLGLQCAVIEFSR
                     DACGLAGANSSEFDPHTPHPVIDLMDEQRDIVDLGGTMRLGIYPARLSPGSIVHDAYG
                     EEVIYERHRHRYEVNNRYRARLEEAGLVCSGTSPDSRLVEFVELGRDTHPFYVSTQAH
                     PEFTSRPDRPHP"
     gene            complement(1636..>2180)
                     /locus_tag="VIJ44_07610"
     CDS             complement(1636..>2180)
                     /locus_tag="VIJ44_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEY5165800.1"
                     /translation="VTGHLRELAMPHAVLTVDVSAASLTDDGSDAVTFLLAPNPGEPP
                     KPVARAASGGELSRAMLALRLVLTEGPPTLVFDEVDAGIGGEAGTAVGRLLADLASRH
                     QVLCVTHLAQVAAHAQTQVVVDKREIAGRTVTAASVVDGDARVAELSRMLAGLVDSAH
                     AQRHAAELIERAAGARNLTR"
BASE COUNT          338 a          757 c          731 g          354 t
ORIGIN      
        1 cggatgaggc cggtcggggc ggctcgtgaa ctccgggtgg gcttgcgtcg acacgtagaa
       61 cgggtgcgtg tcacgaccga gctcgacgaa ctccaccaga cgcgagtcgg gcgaggtgcc
      121 gctgcacacc agcccggcct cctcgagccg ggcccggtac cggttgttca cctcgtatcg
      181 gtgccggtgc cgttcgtaga tgacctcttc tccgtacgcg tcgtgcacga tcgaccccgg
      241 gctgagacga gccgggtaga tgccgagccg catcgtccca ccgaggtcga cgatgtcacg
      301 ctgctcatcc atcaagtcga tgaccggatg cggggtgtgc gggtcgaact cgctcgaatt
      361 ggcaccggcg agcccgcacg cgtcgcgcga gaactcgatc accgcgcact gcaacccgag
      421 acacaacccg aggtatggga tctgatgctc tcgcgcgtac ccggcggcgg cgatcttgcc
      481 ttcgatcccg cgcaccccga acccgccggg gatgacgatg ccgtcgagct cccgcagccg
      541 tccctcggcg aggagccctt cggcgtcgtc ggacgcgatc cagtccagct cgatcctcgc
      601 cccgcacccg tacccgccgt gccggatcgc ttccgcgacc gacaggtacg cgtcgggaag
      661 gttcacgtac ttgccgacga tgccgatccg caccgtctgc tcggcgctct cgatgcggct
      721 caccagagcc cgccacgacg aaagatcggg ctcgtgatgg tcgagttgca ggacccggca
      781 cacgtaattg tcgagccctt cgtcgtggag gacgagcggg atctcgtaga ggttggaggc
      841 gtcgatcgcc gagacgatcc cctcctcggg gacgtcacag agctgggaga tcttctcctt
      901 gagccggatc gggatcggcc ggtcggagcg gcacacgatg gcgtccggct ggatgccgcg
      961 gctgcgtagc tcagtgaccg aatgctgggt cggcttggtc ttctgctccc ccgacgggcc
     1021 gatgaagggg accagcgtca catgcacgta gaacacgttg tcgcgcccga cgtccttgcg
     1081 gaactgtcgg atcgcctcga ggaacggcag gatctcaatg tcaccgacgg tgccccccac
     1141 ctcggtgatg accacgtcga cgtcgtcgtc ggcgaggcgc aggatccggt ccttgatctc
     1201 gttggtgatg tgcgggatca cctgcacggt ctgacccagg tacgcgccgc gccgctcctt
     1261 ggccagcacc tcctggtaga tcgacccggt cgtggcgtta gacttcttgg tcaacggcac
     1321 gtccacgaac cgctcgtagt gaccgagatc gaggtcggtc tcgcctccgt cttcggtgac
     1381 gaacacctcg ccgtgctgga acgggttcat cgtcccgggg tcgacgttga tgtacgggtc
     1441 gagcttctga agcgtgacgc gcaacccgcg gctcttgagc agtcgaccga gcgaagcggc
     1501 ggtgagtcct ttccccagac tggaagcgac cccaccggtc acgaagatgt gcttcgccac
     1561 gggcccaaaa cctatcacga ggtcgcgaca attctcgtga tcggccagaa acacgcgcgc
     1621 ctgcccccgg cgggctcagc gggtgaggtt gcgtgccccc gcggcccgtt cgatcagctc
     1681 cgcggcgtgc cgttgcgcgt gcgccgagtc gacgagcccc gcgagcatcc gcgacagctc
     1741 cgccacccgg gcgtcgccgt ccacgacgct cgccgcggtg acggtacgcc cggcgatctc
     1801 gcgcttgtcg accacgacct gggtctgggc gtgcgccgcg acttgggcca gatgggtcac
     1861 gcagagcacc tgatggcgtg acgccaggtc ggcgaggagc cggccgaccg cggtgccggc
     1921 ctctccgccg atcccggcgt cgacctcgtc gaacaccagc gtcggtggcc cctcggtgag
     1981 gacgagccgc aacgccaaca tggcgcgcga cagctctccc cccgacgcgg cccgggcgac
     2041 cggcttcggc ggctcccccg ggttgggggc gaggaggaac gtgaccgcgt cggacccgtc
     2101 gtcggtcaga ctcgccgcgg acacgtcgac ggtgaggacc gcgtggggca tcgccagctc
     2161 ccgcagatgc ccggtcaccg
//
LOCUS       DATCCH010000395         2573 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_440444, whole genome shotgun sequence.
ACCESSION   DATCCH010000395 DATCCH010000000
VERSION     DATCCH010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440444"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1393
                     /gene="rpsA"
                     /locus_tag="VIJ44_07615"
     CDS             <1..1393
                     /gene="rpsA"
                     /locus_tag="VIJ44_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015748244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEY5165801.1"
                     /translation="TIVEFDDGDIVTGKVVKIDSDEVLLDIGYKSEGVIPSRELSIRN
                     DIDPSEVVTLDEELEALVLTKEDKDGRLILSKKRAQYERAWGTIEELKNTEGIVSGPV
                     IEVVKGGLILDIGLRGFLPASLVDLRRVRDLQPFVGKTLECKIIELDKNRNNVVLSRR
                     AYLEETQREQRDEFLANLKPGEIRHGVVSSVVNFGAFVDLGGMDGLVHVSELSWKHVD
                     HPSSVVTVGDEVTVQVLDVDLSRERISLSLKATQQDPWQEFADGHQVGELVYGRVTKL
                     VPFGAFVQVGEGIEGLVHISEMAGHHVEAPEQVVTPGEELWVKIIDIDLERRRISLSI
                     KQAAEGGVVAAEYQDQYGEHAYDAQGNYIGPDYGTGEAPAAPAGEGGDVAAAQAEGAA
                     DTVASLETAAETSAETAAETPAETSVETPAETSAETPAETIPESDAATGAKAPAVSLV
                     DGEPSAEGTPPSG"
     gene            1532..2251
                     /gene="cpaB"
                     /locus_tag="VIJ44_07620"
     CDS             1532..2251
                     /gene="cpaB"
                     /locus_tag="VIJ44_07620"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HEY5165802.1"
                     /translation="MGNRRTLIAAAAMILAAVAGIGVYFYVSSADQRAQQKVAVVDAF
                     VASQDIPKGTTGENAVANGLITASKVLRGSVPPGAVNDTSLLKGKVAASTIGAKQFIT
                     GSSFVAPSQGGGGSLAAAIGTNGLVAVTVSVDAAHGVADGIAPGDHVDIAVAGDTGAQ
                     YLLTGVRVLAVGQETTASAQGGNGQPSTTAANSGLITFEVSPADALRVVAANRSGSLY
                     LTLESLGTNTGNTPSVPASGR"
     gene            2255..>2573
                     /locus_tag="VIJ44_07625"
     CDS             2255..>2573
                     /locus_tag="VIJ44_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165803.1"
                     /translation="MAVEYGSMAGSGYQPAPARYDVAIIEPDGRRRLRLGTALATAAQ
                     FDAVEELVQSLRPGRPVVAVFGPGLAAPYGFQQVNRLIATYPDLGVIFAVDEVSTPLL
                     QAAI"
BASE COUNT          439 a          878 c          889 g          367 t
ORIGIN      
        1 cacgatcgtc gagttcgacg acggcgacat cgtgaccggc aaagtcgtga agatcgacag
       61 cgacgaagtc ctgctcgaca tcggctacaa gtccgaaggg gtcatcccct cacgcgagtt
      121 gtcgattcgc aatgacatcg acccgagcga ggtcgtgacc ctcgacgagg agctcgaggc
      181 cctggtcctc accaaagagg acaaagacgg gcggctcatc ctctccaaga agcgggccca
      241 gtacgaacgg gcctggggca ccatcgagga gctcaagaac accgagggca tcgtctccgg
      301 tccggtcatc gaagtcgtca aaggcggcct gattctcgat atcgggttgc ggggcttcct
      361 ccccgcgtcg ctcgtcgacc tgcgccgggt ccgggaccta cagcccttcg tcggtaagac
      421 cctcgagtgc aagatcatcg agctcgacaa gaaccgcaac aacgtcgtct tgtcgcggcg
      481 cgcgtacctc gaggagaccc aacgggagca gcgcgacgag ttcctcgcca acctcaagcc
      541 cggtgagatt cgtcacggtg tcgtctcgtc ggtcgtcaac ttcggtgcct tcgtcgacct
      601 cggcggcatg gacggcctgg tgcacgtttc agagctgtcg tggaagcacg tcgatcaccc
      661 cagctcggtg gtgacggtcg gtgacgaggt cacggtccag gtcctcgacg tcgacctgtc
      721 ccgggagcgc atctccctct ccttgaaagc cacccagcag gacccatggc aggagttcgc
      781 cgacgggcat caggtcgggg agctggtcta cgggcgggtc acgaagctcg tcccgttcgg
      841 cgcgttcgtg caggtcgggg agggaatcga agggctggtc cacatctccg agatggccgg
      901 ccaccacgtc gaggcccccg agcaggtcgt caccccgggc gaggagctct gggtgaagat
      961 catcgacatc gacctcgagc ggcgccgaat ttcgctctcg atcaagcagg ccgccgaagg
     1021 cggcgtcgtc gccgccgagt accaggacca gtacggcgag cacgcctacg acgcccaggg
     1081 caactacatc ggacccgact acggcaccgg cgaagccccc gcggcccctg ccggcgaggg
     1141 tggagacgtc gcggcggcgc aagccgaggg cgcggccgac accgtggcca gcctcgaaac
     1201 cgccgccgag accagcgccg agaccgcggc tgagacccca gctgagacca gcgtcgagac
     1261 cccagctgag accagcgccg agaccccagc tgagaccatc cccgaaagcg acgccgcgac
     1321 gggggccaag gccccggccg tcagcctggt ggacggcgag ccgtcggccg aggggacccc
     1381 accctcgggg tgaaccctcg acggccacgt gacccgagcc caccgggtcg cggtgccccc
     1441 ggttggccct caaggtccgc cgggtgccgg ccgaccatga agtcgggact gcccttgggg
     1501 cgctagggag acccgaccga ggagcgagcc tgtgggtaat cgccgcaccc tgatcgcggc
     1561 cgccgccatg atcctcgccg cggtggcggg aatcggcgtc tacttctatg tgtcctctgc
     1621 ggaccaacgg gcccagcaga aagtggccgt ggtcgacgcg ttcgttgcgt cccaggacat
     1681 cccgaagggg accaccgggg agaacgccgt cgcgaacggg ttgatcaccg cgtcgaaggt
     1741 cctgcggggc agcgtgccac ccggcgcggt caacgacacc agcctcttga aaggcaaggt
     1801 cgcggcctcg acgatcgggg caaaacagtt catcaccggc tcgagcttcg tcgccccctc
     1861 ccagggtggc ggcgggtcgc tggccgcggc gatcgggacc aacggtctgg tggcggtcac
     1921 ggtgtcggtc gacgccgcgc acggcgtcgc cgatggcatc gcccccgggg accacgtcga
     1981 catcgcggtc gccggtgaca ccggcgccca gtatctgctg accggggtcc gggtcctcgc
     2041 ggtgggccaa gagaccacgg cgagcgccca gggcggcaac ggtcaaccga gcacgaccgc
     2101 agcgaacagc gggttgatca ccttcgaggt gtcgcccgcc gacgcgctgc gtgtggtggc
     2161 cgcgaaccgc agcggctcgc tgtacctgac gttggagtcg ctggggacca acacaggtaa
     2221 cacgccttcg gtcccagcta gcggacggta gggcatggcc gtcgagtacg gcagcatggc
     2281 gggcagcggc taccaaccgg cgccggcgcg ctacgacgtc gcgatcatcg aacccgatgg
     2341 gcggcgccgg ttgcggctcg gcaccgcgct ggcgacggcg gcgcagttcg acgcggtcga
     2401 ggagctggtg cagtcgctgc gtccgggtcg cccggtggtg gcggtcttcg ggccgggtct
     2461 cgccgcgccg tacgggttcc agcaggtcaa ccggctgatc gccacgtacc ccgacctggg
     2521 tgtgatcttc gcggtcgacg aggtgtcgac ccctttgctg caggccgcga tcc
//
LOCUS       DATCCH010000396         4651 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_440662, whole genome shotgun sequence.
ACCESSION   DATCCH010000396 DATCCH010000000
VERSION     DATCCH010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4651
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440662"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3705)
                     /locus_tag="VIJ44_07630"
     CDS             complement(<1..3705)
                     /locus_tag="VIJ44_07630"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HEY5165804.1"
                     /translation="MTHHESHPVAVVGMSAIMPDAPSADAFWHNITAGRYSISPTPAD
                     RWDQALYYDEDHSVPDKTYSVIGGWVREFDWNPAAWRLPLPPMVAAQMDEGQRWAISA
                     ARAALTDAGWPGWPVDSDRVAVILGNALGGEKHYQSSMRIRFPEMLRRLEQSATLATL
                     APETREHIIEETRRGYLDNVFAITEDTMPGELANVIAGRVANLFNLRGMNYTTDAACA
                     SGLAALNSAVTGLADHQFDAVVTGGIDHNMGVEAFVKFCKIGALSATGTRPFDAGADG
                     FVMGEGAALFVLKRLEDAERDGDRVYAVVLGIGGSSDGKGKGITAPNPVGQQLSVQRA
                     WERAGVDPSTAGAVEAHGTSTRVGDASELTSLTAVFGASGAAPGSIALGSVKSNIGHL
                     KAAAGAAGLFKMVRSLHEKQLPPSLNFVDPNPNVDWDTLPFAVTTELRPWPEPAKDVR
                     RGGVSAFGFGGTNFHVVLEEHVPGRHRPPARSFASAGIPGAAPAPAGAPPTSQGATTP
                     GAGASASVGAAKAPLRGALVLGGRDDADLASQVQAALAQARAGVTPARTAPNPALASA
                     AVRVAVDHTDAADLATKLTKLANAFASGNPATFRLLRQQGIFLGRGPAPKAAFLYTGQ
                     GSQYVNMLKSLAAEEPVVKATFDEADRVMTPLLGRPLTSFIFVDGDDPAAVQTLNQQL
                     MQTEITQPAVLAADISLTRLLAAYGVQPDMVMGHSLGEYGALVAAGALTLDSALEAVS
                     ARGREMTKVSMADNGAMAAVFAPMAEIQRIVDQADGYVVIANINSTSQAVVGGATDAV
                     ERIIERFTAEGFTATRIPVSHAFHTSIVAPASVPFVDALRRLDLRPPTMPVVANVTGD
                     FYPQGATTETVLEYAGKQIASPVQFVKGLQSLYAAGARVFVEVGPKKALHGFVEDVLS
                     SEHDDVVALFTNHPKVGDDVAFNQALCGLYAAGIGLDRHETPEVPVGSTAPTARSTSP
                     AETTATQTVDALDAPADATIRELGLLFADVLEKGMHLYAGTGAAGWQPSAPPAPVPAP
                     APEPARPAGPPAVEPVVITGAALGLPGTEHIFDDDNVAKILAGQNFISVLGDDARQRM
                     VDMRITRLVKDAASGGGSFATIDNPQDVIKLAGVLAPLDVVEQFGVDKARDDALDTTT
                     RLAIGAGFDALRDAGIPLVMSYKTTTLGTQLPDRWGLPEALRDETGVIFASAFPGYNR
                     FAEALEGYALNRGRREGLLAVEALRARMPDE"
     gene            complement(3781..>4651)
                     /locus_tag="VIJ44_07635"
     CDS             complement(3781..>4651)
                     /locus_tag="VIJ44_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY5165805.1"
                     /translation="YSMQREQGGGPLSEKQGFTHKLIVPHAVRLEAARAFMEDTADRL
                     DRGLGDNGAMNTEGAIAKYMATEAGNAAADAAIQAHGGYGYTRPYVVEKIRRDVRITT
                     IYEGTSEIMEMTIARGRWQAHLKSGGRHYLDVATEMASLHVRNPLVGADSASLAARSL
                     AVVLDCCRVGRLTRNQHILFRIGELVAATETAVVFAQAADQAMAGRRFEKSPTRFTGD
                     ALAAMSRVFARESAHKVALDGSRWVAGALEPDSPVLAGLDGIPADAIRTAQGGLVADM
                     DLVADVLYGRTPA"
BASE COUNT          618 a         1521 c         1802 g          710 t
ORIGIN      
        1 ttcgtcgggc atccgggcgc gcaacgcctc gacggccagc aggccctccc ggcgaccgcg
       61 gttgagcgcg tagccctcga gggcctccgc gaagcggttg tagccgggga aggccgaggc
      121 gaagatgacc ccggtctcgt cccgcagcgc ttccggcagg ccccatcggt cgggcagctg
      181 ggtgcccagc gtcgtggtct tgtagctcat gacgagcggg atgccggcgt cgcgcagggc
      241 gtcgaagccg gccccgatcg ccagccgcgt cgtggtgtcg agggcatcgt cgcgggcctt
      301 gtcgaccccg aactgctcga cgacgtccag gggtgcgagc accccggcga gcttgatgac
      361 gtcctgaggg ttgtcgatcg tcgcgaagct cccgcccccc gaggcggcgt ccttgaccag
      421 ccgggtgatc cgcatgtcga ccatccgctg gcgggcgtcg tcgccgagga cgctgatgaa
      481 gttctgaccg gcgaggatct tcgcgacgtt gtcgtcgtcg aagatgtgct cggtcccggg
      541 cagcccgagg gccgcacccg tgatgacgac gggctccacg gccggcggac ccgccggccg
      601 ggccggctcc ggagcgggag caggaaccgg ggccgggggc gcactcggct gccagccggc
      661 ggcaccggtc ccggcataca ggtgcatgcc cttctcgagc acgtcggcga agagcagccc
      721 gagctcacgg atcgtggcgt cggccggggc gtccagcgcg tcgaccgtct gcgtcgcggt
      781 cgtctccgcc ggggacgtgg accgcgcggt cggcgcggtc gagccgacgg ggacctccgg
      841 ggtctcgtgc cggtccagac cgatgccggc cgcgtagagc ccgcacaggg cctggttgaa
      901 cgccacgtcg tctcctacct tcgggtggtt ggtgaagagc gcgacgacgt cgtcgtgctc
      961 ggagctgagc acgtcctcga cgaagccatg cagcgccttc ttcgggccga cctcgacgaa
     1021 gacgcgagcg ccagcggcat acaggctctg caggcccttg acgaactgga cgggggaggc
     1081 gatctgtttc ccggcgtact ccaggactgt ctcggtcgta gcgccctggg ggtagaagtc
     1141 acctgtcacg ttggcgacga cgggcatggt cggcggcctg aggtcgagcc ggcgcagcgc
     1201 gtcgacgaac ggcacggagg ctggcgcgac gatcgaggtg tggaaggcgt ggctgaccgg
     1261 gatccgggtc gctgtgaagc cctctgcggt gaagcgctcg atgatccgct cgacggcgtc
     1321 ggttgcgccg ccgacgacgg cctggctcgt gctgttgatg ttcgcgatga cgacgtagcc
     1381 gtcggcctgg tccacgatcc gctggatctc ggccatcggc gcgaacaccg cggccatggc
     1441 gccgttgtcg gccatggaca ccttggtcat ctcgcggcca cgggcgctga ccgcctccag
     1501 ggcgctgtcg agggtcagcg cgccggcggc gacgagggcg ccgtactcac cgaggctgtg
     1561 ccccatcacc atgtcgggct gcaccccgta tgccgcgagc agccgggtca gcgagatgtc
     1621 ggcggcgagc accgcaggct gggtgatctc ggtctgcatc agctgctggt tgagcgtctg
     1681 cactgctgcc gggtcgtcgc cgtcgacgaa gatgaacgag gtcagaggcc gccccaggag
     1741 cggggtcatc acccggtccg cttcgtcgaa ggtcgccttg accacgggct cctcggcggc
     1801 cagggacttg agcatgttga cgtactgcga gccctgcccg gtgtaaagga aggcggcctt
     1861 gggggcaggg ccgcgcccga ggaatatccc ctgctggcgc agcaaccgga acgtcgcggg
     1921 gttgcccgag gcgaacgcgt tggcgagctt ggtgagcttg gtcgcgaggt ccgccgcgtc
     1981 ggtgtggtcg accgcgaccc ggacggccgc ggaggccagc gccgggttcg gcgcggtgcg
     2041 cgcgggtgtg acaccggcgc gggcctgtgc caaggcagcc tgcacctggg aggcgaggtc
     2101 ggcgtcgtcg cggccaccga ggaccagggc gccgcgcagg ggggccttcg ctgcgccgac
     2161 ggaggcggac gcaccggctc ctggggtggt ggcgccctgg ctcgtgggag gtgcgccggc
     2221 cggcgcgggt gccgcacccg ggatgccggc ggatgcgaag ctcctcgcgg gcgggcggtg
     2281 ccgcccgggc acgtgctcct cgaggacgac gtggaagttc gtgccgccga agccgaaggc
     2341 actgacgccc ccgcgacgca cgtccttcgc cggctccggc caggggcgca gctcggtggt
     2401 gacggcgaac gggagggtgt cccagtcgac gttggggttg gggtcgacga agttcaggct
     2461 cggggggagc tgcttctcgt ggagcgaccg caccatcttg aacaacccgg cagcgcccgc
     2521 ggcggccttg agatggccga tgttcgactt caccgacccg agggcgatcg agccgggggc
     2581 ggcaccggag gcgccgaata cagcggtgag gctggtcagc tccgaggcgt ctccgacacg
     2641 ggtggaggtg ccgtgggcct cgacggcccc ggccgtcgag gggtcgacac cggcgcgctc
     2701 ccaggcgcgc tggacagaga gctgctggcc gacggggttc ggcgccgtga tgcccttgcc
     2761 cttgccgtcg gacgagccgc cgatgccgag gacgacggcg tagacgcggt ccccgtcgcg
     2821 ttcggcgtcc tcgagccgct tgagcacgaa caaggctgcg ccctcgccca tgacgaaacc
     2881 atcggccccg gcgtcgaatg gccgggtgcc ggtggccgag agggcaccga tcttgcagaa
     2941 cttcacgaac gcttccacgc ccatgttgtg gtcgatgccg ccggtgacga cggcgtcgaa
     3001 ctggtggtcg gcgaggccgg tgacggcgga gttcagggcg gccaggcccg aggcgcacgc
     3061 ggcatccgtg gtgtagttca tcccgcgcag gttgaacagg ttcgcgaccc tgccggcgat
     3121 gacgttcgcg agctcaccgg gcatggtgtc ctcggtgatc gcgaagacgt tgtcgaggta
     3181 gccgcgccgc gtctcctcga tgatgtgctc gcgggtctcg ggggcgaggg tggcgagcgt
     3241 ggccgactgc tcgagccgcc tgagcatctc cgggaacctg atccgcatgc tcgactggta
     3301 gtgcttctcg ccgccgaggg cgttgcccag gatcaccgcg acccggtcgc tgtcgaccgg
     3361 ccagccgggc cagccggcgt cggtgagggc cgcccgggcg gcggagatgg cccagcgctg
     3421 gccctcgtcc atctgtgccg cgaccatcgg tgggagcggc agccgccagg cggccgggtt
     3481 ccagtcgaac tcgcgcaccc agccgccgat cacgctgtac gtcttgtcgg ggacgctgtg
     3541 gtcctcgtcg tagtagaggg cctggtccca gcggtctgcc ggggtcggcg agatggagta
     3601 cctgccggcg gtgatgttgt gccagaacgc atccgcgctg ggtgcgtcgg gcatgatcgc
     3661 cgacatgccg acgacggcga ctgggtggct ctcgtgatgc gtcatggtgg ggtccgaatc
     3721 ggtgtggttg cggttcgagg tcgggtgggg tcgcgatgct gaggggtcgg cgggaaggcg
     3781 tcaggcgggg gtgcggccgt acagcacgtc ggcgacgagg tccatgtcgg cgaccaggcc
     3841 gccctgggcg gtgcggatgg cgtcggcggg gatgccgtcg agcccggcga ggaccgggct
     3901 gtcgggttcc agcgcgccag cgacccaccg ggatccgtcg agggcgacct tgtgcgcgga
     3961 ctcccgggcg aagacccggg acatggcggc gagggcgtcc ccggtgaagc gggtcgggga
     4021 cttctcgaag cggcggccgg ccatcgcctg gtccgccgcc tgagcgaaga ccacggcggt
     4081 ctcggtggcg gcgacgagct cgccgatccg gaagaggatg tgctggttgc gggtcagccg
     4141 tcccacgcgg cagcagtcga ggacgacggc gagggaccgc gccgcgagcg aggcggagtc
     4201 ggcgccgacg agcgggttgc ggacgtgcag cgacgccatc tcggtcgcca catcgaggta
     4261 gtggcggcca ccggacttca gatgggcctg ccaacgtccc cgggcgatgg tcatctccat
     4321 gatctcggag gtgccctcgt agatcgtcgt gatccgcacg tcgcggcgga tcttctccac
     4381 gacgtagggc cgggtgtagc cgtacccgcc gtgtgcctgg atcgcggcgt cggcggcggc
     4441 gtttcccgcc tcggtggcca tgtacttggc gatggcgccc tcggtgttca tcgcgccgtt
     4501 gtcgccgagg ccgcggtcga gccggtccgc cgtgtcctcc atgaacgcgc gggccgcctc
     4561 tagacgcacg gcgtgcggga cgatgagctt gtgcgtgaag ccctgtttct cgctgagcgg
     4621 gccgccaccc tgctcgcgct gcatcgagta g
//
LOCUS       DATCCH010000397         3668 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_607550, whole genome shotgun sequence.
ACCESSION   DATCCH010000397 DATCCH010000000
VERSION     DATCCH010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3668
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607550"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..562
                     /locus_tag="VIJ44_07640"
     CDS             173..562
                     /locus_tag="VIJ44_07640"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY5165806.1"
                     /translation="MTLTDDDARELFDRRRTAWLAGDVDAYLDCWVDDLVLETPGRVI
                     RGRADYEQMVRGSLGWAAPQSFVVHHLAVDGDVVLADWTITVERRDDREPVTWWGMSA
                     CELRDGRIVWWREYYQDPAALGRAARR"
     gene            577..1725
                     /locus_tag="VIJ44_07645"
     CDS             577..1725
                     /locus_tag="VIJ44_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006380182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY5165807.1"
                     /translation="MDLTFTPTEETFRAECRTWLEANVPRGLPSGDTRAGFAAHLDWE
                     RALSDARWAVVSWPDRYGGRDASPMEWLIFEEEYYRAGGPQRVTQNGIFLLAPTVFEF
                     GTAEQQDRVLPRMASAQDLWCQGWSEPNAGSDLAGIQSRAARDDVAGGWRLSGQKTWT
                     TRGAFCTHLFGLFRSDPDAERHRGLTYFLVPLAAPGITVRGFGRLDGDEGFAEVFLDD
                     VFVADHDVLGAVHDGWNVAMATTSSERGLTLRSPGRFLATAERLNRLFRARGADADPA
                     LADAVVRAWIDAEAYRWQTFFTVTRMTDGESLGAESSLTKVFWSELDVRLHEHALELL
                     GSEAEWDDGDDRAWVKGYQFALSGPIYAGTNEIQRNVIAERVLGLPRR"
     gene            1729..2706
                     /locus_tag="VIJ44_07650"
     CDS             1729..2706
                     /locus_tag="VIJ44_07650"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEY5165808.1"
                     /translation="MRFAFTEEQLAVRDAARDLLAHECPPAVVRRAWDGDARRTDAWG
                     HLAEMGVLGALAPETAGGLGLGFVDLILVLEETGYVALPDPIVEHAAVAVPLLADPAP
                     AATGAQTLTATLDGSRIAVWADTADVVAVGDDTGQRLVSRDTATLEPVRSVDGARRLF
                     RVGPIPDDAPTVSDLHAFDRGALGVAAQLVGLGRRMLDLTVGYASERRQFGVPIGSFQ
                     AVKHQLADARIALEFARPLVYRAAWSLDQRDADASLHVSMAKAQASDAAHLAGRAALQ
                     CHGAIGYSYEYDLHLYLKRAWALEASGGDAGWHRRRVGRAIGVVTPGRT"
     gene            2777..>3668
                     /locus_tag="VIJ44_07655"
     CDS             2777..>3668
                     /locus_tag="VIJ44_07655"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010039486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEY5165809.1"
                     /translation="MPEAYLVDAVRTPVGKKGGGLSAVHPADLGAHVIRALVERSDLD
                     PLAVEDVFFGCVDQLGPQAGDLARTCWLAAGYPEEVPGTTIDRQCGSSQQALHFAAQA
                     VMSGTNDVIVAGGVQNMTMIPIAAALTAAEPFGFEDPFSGSEGWVARYGTQEVSQFRG
                     AELIAEKWGITREEMEGFALESHERAIRAQDESRFEHEIVPLGDARADEGPRRGTSLE
                     KMAQLPTLVDGGQLTAAVSSQISDAASAMLVMSEQAVRDHGVTPRARVHHLSVRGADP
                     IYMLTAPIPATAYALAKTGMS"
BASE COUNT          505 a         1397 c         1263 g          503 t
ORIGIN      
        1 gctgccgcgt gcgcgtcgtg agcaccggcc cgacccggcg cgggcggcgc tccaacaccg
       61 ccaccgcggc gatcccgacg agcgacgtgg cgaacgacac gagagcgata gtcatcatct
      121 cgtccaacgt cgccgcgacg agtcctgttc cccggtccgg tctcggcacc cgatgacgct
      181 caccgacgac gacgcccggg aactgttcga ccggcggcgc accgcctggc tggccggcga
      241 cgtcgacgcc tacctcgact gctgggtcga cgacctggtc ctcgagacgc ccggccgggt
      301 catccggggc cgcgccgact acgagcagat ggtgcgaggg tcgctcggct gggccgcgcc
      361 acagtcgttc gtcgtgcatc acctcgcggt cgacggcgac gtcgttctcg ccgactggac
      421 catcaccgtc gaacgtcgcg acgaccgcga gcccgtgacg tggtggggca tgagcgcgtg
      481 cgagctgcga gacggccgga tcgtgtggtg gcgcgagtac taccaggacc cggcggcgct
      541 gggccgagca gcgcgccggt agcgtctctc gccgccatgg atctgacgtt cacccccacc
      601 gaggagacgt tccgcgccga gtgccggacg tggctcgagg cgaacgtgcc ccgcggcctt
      661 ccgtcgggtg acacccgagc cgggttcgcg gcccacctcg actgggaacg ggcgctctcc
      721 gacgcccgct gggccgtcgt gtcgtggccg gaccgctacg gcggacgcga cgcgtccccg
      781 atggagtggc tcatcttcga ggaggagtac taccgagccg ggggccccca acgcgtcacg
      841 cagaacggga tcttcctgct cgccccgacc gtcttcgagt tcggaaccgc cgagcaacaa
      901 gaccgggtcc ttccccgcat ggcttcggcc caggacctgt ggtgccaggg ctggtccgaa
      961 cccaacgccg gcagcgacct cgccggaatc cagagccggg ccgcacgcga cgacgtcgcg
     1021 ggcggctggc gactgtcggg tcagaagacc tggaccaccc gcggcgcgtt ctgcacccac
     1081 ctcttcggcc tgttccgaag cgatcccgac gccgaacgtc accgcggcct cacctacttc
     1141 ctggtccccc tcgccgcgcc cgggatcacc gtccggggct tcggtcgcct cgacggtgac
     1201 gaagggttcg ccgaggtgtt cctcgacgac gtgttcgtcg ctgaccacga cgtgctcggc
     1261 gccgttcatg acggctggaa cgtcgccatg gccacaacga gctccgagcg gggcctcacc
     1321 cttcgcagcc cgggccggtt cctcgccacc gccgagcgcc tgaaccggct cttccgagct
     1381 cgcggggccg acgccgatcc ggccctcgcc gacgcggtcg tccgcgcctg gatcgacgcc
     1441 gaggcctacc ggtggcagac cttcttcacc gtcacccgca tgaccgacgg cgagtccctc
     1501 ggcgccgaat cgagcctgac caaagtgttc tggtccgagc tcgacgtgcg cctccacgag
     1561 cacgcgctcg agctgctcgg ctccgaggcc gagtgggacg acggcgacga ccgggcctgg
     1621 gtcaagggct accagttcgc cctgtccgga ccgatctacg ccggcacgaa cgagatccaa
     1681 cgcaacgtca tcgccgagcg cgtgctcggg ctcccccgcc ggtagcgcgt gcggttcgca
     1741 ttcaccgagg agcagctcgc ggtccgcgac gcggcgcgtg acctgctcgc gcacgagtgc
     1801 cccccggcgg tggtccgccg cgcctgggac ggcgacgccc gacgcaccga cgcgtggggg
     1861 cacctggccg agatgggagt gctcggcgcg ctcgcacccg agaccgcggg tggactcggg
     1921 ctcggcttcg tcgacctgat cctggtcctg gaggagaccg gctacgtggc gctgcccgac
     1981 ccgatcgtcg agcacgcggc cgtggcggtc ccgctgctgg ccgaccccgc gccggccgcg
     2041 accggagcgc agacactcac ggccacgctc gacgggtccc gcatcgcggt ctgggccgac
     2101 accgccgacg tcgtcgcggt cggcgacgac accggccagc ggctcgtctc ccgcgacact
     2161 gccacgctcg agccggtgcg gtcggtcgac ggcgcccgcc ggctcttccg ggtgggcccg
     2221 attcccgacg acgcgccgac cgtatccgac ctccacgcct tcgaccgcgg cgcgctcggc
     2281 gtcgcggcgc agctcgtcgg gctcgggcga cggatgctgg acctgaccgt cgggtacgcg
     2341 agcgagcggc gccagttcgg tgtccccatc ggctcgttcc aggcggtcaa gcaccagctg
     2401 gccgacgccc gcatcgcact cgagttcgcg cgcccgctcg tctaccgggc ggcctggtcc
     2461 ctcgaccaga gggacgccga cgcgtcgctg cacgtctcga tggcgaaggc ccaggcgtcc
     2521 gacgccgcgc acctcgccgg gcgagccgcg ctccagtgcc acggcgccat cggctactcc
     2581 tacgagtacg acctgcatct ctacctgaag cgcgcctggg ccctcgaggc cagcgggggc
     2641 gacgccggct ggcaccgccg tcgggtgggt cgggcgatcg gggtggtgac gcccggccgg
     2701 acgtgacgcc gcacccccgg tcggggccgg ccggccgggc cgtatcgtgg tggcccgtcc
     2761 acacccagga gcagtgatgc ctgaggccta cctcgtcgac gccgtgcgca ccccggtggg
     2821 caagaagggt ggcggcctct ccgcggtgca tcccgccgac ctcggcgccc acgtgatccg
     2881 ggccctcgtg gaacgcagcg acctcgaccc cctggccgtc gaggacgtct tcttcgggtg
     2941 cgtcgaccag ctcggccccc aggccggtga cctcgcccgc acctgctggc tcgccgccgg
     3001 gtaccccgaa gaggttcccg gcaccaccat cgaccgccag tgcggctcgt ctcagcaggc
     3061 gctgcacttc gccgcccagg ccgtcatgag cggcaccaac gacgtgatcg tggccggcgg
     3121 cgtccagaac atgaccatga tcccgatcgc ggcggcgttg accgccgccg agccgttcgg
     3181 gttcgaggac ccgttctcgg gctcagaggg ttgggtcgcc cggtacggca cccaggaggt
     3241 gtcgcagttc cgaggcgcgg agctgatcgc cgaaaagtgg ggcatcaccc gcgaggagat
     3301 ggagggattc gccctcgaga gccacgaacg ggccatccgg gcccaggacg agagccgctt
     3361 cgagcacgag atcgtccccc tgggcgatgc ccgagccgac gaggggcccc gccggggcac
     3421 ctcgctcgag aagatggccc agcttcccac cctcgtcgac gggggccagc tgaccgccgc
     3481 ggtgtcgtct cagatctcgg acgcggccag cgcgatgctc gtcatgtccg agcaggcagt
     3541 ccgggaccac ggggtcaccc cccgagcccg ggtccatcac ctctcggtcc gcggcgcaga
     3601 cccgatctac atgctgaccg ccccgatccc ggccaccgcg tacgcgctcg ccaagaccgg
     3661 gatgtccc
//
LOCUS       DATCCH010000398         4141 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_608443, whole genome shotgun sequence.
ACCESSION   DATCCH010000398 DATCCH010000000
VERSION     DATCCH010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608443"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            6..455
                     /locus_tag="VIJ44_07660"
     CDS             6..455
                     /locus_tag="VIJ44_07660"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:135..137,aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY5165810.1"
                     /translation="MGDPAPDFTVPGVENGQRRDFHLADYRGQKVVLAFYPGDFTPGU
                     TRQLRSYRDDFAVFEQAGAVVLAISPQDVDSHERWAEQEGFKFALLADPDKHVIDDYG
                     IASRLTGVRRSIFLIDTAGIVQWRYTGTVRAIFKKPQQLTRILNRMS"
     gene            455..865
                     /locus_tag="VIJ44_07665"
     CDS             455..865
                     /locus_tag="VIJ44_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009741982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEY5165811.1"
                     /translation="MDLDEARQFLRVNHRAVLATNRSDGRPQLSPVAVAVDPDGRVVV
                     STRATALKANNVARDPHVSLCAFTDGFYGGWVQIDGDADIQTLPDAMEPLVDYYRSVS
                     GDHPDWDDYRAAMQRDRRVLLRITITRAGPDRSG"
     gene            905..1366
                     /locus_tag="VIJ44_07670"
     CDS             905..1366
                     /locus_tag="VIJ44_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010693779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY5165812.1"
                     /translation="MDVSITVNGQAQRHDVEARALLVQFLREDCELTGTKIGCDTSSC
                     GACTVLLDGESVKSCTMLAAQADGAAVTTIEGLATGPEMHPVQRAFQDQHGLQCGFCT
                     AGMVMATVSLLAEVPNPSERDVRLGLEGNLCRCTGYHNIVRAVLAAAGERA"
     gene            1363..2184
                     /locus_tag="VIJ44_07675"
     CDS             1363..2184
                     /locus_tag="VIJ44_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEY5165813.1"
                     /translation="MIPAPFDYVRASTLDDVTAALAEHGDDAKVLAGGMSLLPLMKLR
                     LATPSVLIDVARVPDLSFIRDTGNHLTIGALTRHRDLETSDVLAEHCAVLRAVAAQVG
                     DNQVRHRGTLGGSVAHGDPASDLPAVLLALDATFVARGPDGERSIAAADFFLGFLETA
                     LAPNEVLTEIRVPKTGPTGFSYQKFNRRAQDWAIVGAVAVTNGTTRVGLVNMASTPHR
                     ATEVEAALASGATIDDAAEHAADGTEAPADLNASPEYREHLARVLVRRALTEAQT"
     gene            2203..2796
                     /locus_tag="VIJ44_07680"
     CDS             2203..2796
                     /locus_tag="VIJ44_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015784032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEY5165814.1"
                     /translation="MTVAAALLAAGRGSRLGADATAKPLLELSGRRLVDWALDAAITA
                     GTRPVLLVVGHGRDAVAAAAPPTVQVITATDWGLGIAHSLRAALDALEPHPEIDAVCV
                     GLADQPRVGPDAYRRLVDAHQHGATLAVATYTGQRANPVLLDRSLWPEARTLHGDTGA
                     RALMSTHTITEVDCTGTGDPTDVDTLDDLRALEGRTE"
     gene            2793..3497
                     /locus_tag="VIJ44_07685"
     CDS             2793..3497
                     /locus_tag="VIJ44_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019068117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY5165815.1"
                     /translation="MKLEHNFRVGVPVDEAWTTLLDLERIAPCMPGAQLQEVDGDEYR
                     GIVKVKVGPITAQYKGIARLTEADEAAGRLIITAEGRDTRGQGNASATITARLSADGD
                     GTQVAVDTDLNVTGKVAQFGRGVMADVSSKLLGQFVDCLSTSILGPGAPTTSTPPTPA
                     AAPAPGPSSTPGAAPASSASPASPASPGPRRIDAPEAEAVDLMRAAGGAVAKRLVPLI
                     AAGVVVVGVAVWFLLR"
     gene            3540..4073
                     /gene="ruvC"
                     /locus_tag="VIJ44_07690"
     CDS             3540..4073
                     /gene="ruvC"
                     /locus_tag="VIJ44_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012181989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEY5165816.1"
                     /translation="MFDRDGPVLGVDPGLSQCGYGVVRRDGRRLAAVACGILRTDPGA
                     PLPERLATLEADLDEFLREVGPCALAVERVLFQANVRTAISVGQASGLALAVAARAGV
                     PVTHYSPNEVKLAIAANGAAGKSQVQAMVTRVLRLSEQPPPDAADALALAVCHLWRMP
                     LRASVARAVAADGDRRS"
BASE COUNT          627 a         1666 c         1287 g          561 t
ORIGIN      
        1 tcaccattgg tgatccggca cctgacttca ccgtccccgg ggtggagaac gggcaacgtc
       61 gcgatttcca cctcgctgac taccggggcc agaaggtggt tctcgccttc tatcccggtg
      121 acttcacccc tggctgaacc cgtcagctgc gctcgtaccg cgacgacttc gcggtgttcg
      181 agcaggccgg tgcggtcgtg ctggccatct ccccccaaga cgtcgacagc catgagcgct
      241 gggccgagca agaagggttc aagttcgcat tgctcgccga ccccgacaag cacgtcatcg
      301 acgactacgg gatcgcgtcg cggctcaccg gggtgcggcg atccatcttc ctcatcgaca
      361 ccgccgggat cgtgcagtgg cgctacaccg gtacggtgcg ggccatcttc aagaaacctc
      421 agcagctgac ccgcatcctc aaccggatga gctgatggac ctcgacgagg cccgccagtt
      481 cctccgcgtc aaccaccgcg cggtgctggc caccaaccgg tccgacgggc ggccccagct
      541 ctcaccggtc gcggtcgccg tcgacccgga cggccgcgtc gtcgtcagca cccgagcgac
      601 cgcgctcaag gccaacaacg tcgcacgcga cccacacgtc tccctctgcg ccttcaccga
      661 cgggttctac ggcggctggg tccagatcga cggcgacgcc gacatccaaa ccctccccga
      721 cgcgatggaa cccctcgtcg actactaccg cagtgtcagc ggcgaccacc ccgactggga
      781 cgactaccgc gccgcgatgc agcgggaccg ccgcgtgctg ctgcggatca cgatcacccg
      841 agcgggaccc gaccgctcgg ggtaaaccgg gtagattgct gcccgcccgt cgcccaggag
      901 ccccgtggac gtctcgatca ccgtcaatgg acaagcgcaa cgacacgacg tcgaggcgcg
      961 cgcgctcctc gtccagttcc tccgagagga ctgcgagctg accggcacca agatcggctg
     1021 cgacacgtcg tcgtgcgggg cctgcaccgt cctcctcgac ggcgaatcgg tgaagtcgtg
     1081 cacgatgctc gccgcgcaag ccgacggcgc ggcggtcacc acgatcgaag ggctcgccac
     1141 cggacccgag atgcaccccg tccagcgcgc cttccaggac cagcacggcc tgcaatgcgg
     1201 cttctgcacc gccgggatgg tcatggcgac cgtttcgctc ctcgctgagg tccccaaccc
     1261 gagcgaacgc gacgtccgcc tcggcctcga aggcaacctc tgtcgttgca ccgggtacca
     1321 caacatcgtc cgggcggtcc tcgccgcggc cggggagcgc gcatgatccc cgcccccttc
     1381 gactatgtcc gcgcctccac cctcgacgac gtcaccgcgg cgctcgccga gcacggcgac
     1441 gacgccaagg tcctcgccgg cgggatgtcc ctgctgcccc tcatgaagct ccggcttgcc
     1501 accccctcgg tcctcatcga cgtcgcccga gtgcccgacc tgagcttcat ccgcgacacc
     1561 gggaaccacc tcaccatcgg ggcgctcacc cgccaccgtg acctcgagac cagcgacgtc
     1621 ctcgccgagc actgcgccgt cctgcgtgcc gtcgcggccc aggtcggcga caaccaggtc
     1681 cgacatcgcg gcaccctcgg cggctccgtc gcgcacggcg accccgcctc ggatctcccc
     1741 gcggtcctgc tggccctcga cgccaccttc gtggcccgcg gccccgacgg tgagcgcagc
     1801 atcgccgccg ccgacttctt cctcggcttc ctcgagaccg ccctggcccc caacgaggtc
     1861 ctcaccgaga tccgcgtccc gaagaccggc ccgaccgggt tctcgtacca gaagttcaac
     1921 cgccgggccc aggactgggc cattgtcggc gcggtcgcgg tcaccaacgg caccacccga
     1981 gtcggcctgg tcaacatggc cagcaccccg caccgcgcca ccgaggtgga ggcagccctc
     2041 gcctccgggg ccaccatcga cgacgccgcc gagcacgccg ccgacggtac cgaagccccc
     2101 gcggatctga acgcgtcacc cgagtaccgc gagcatctcg cccgggtact ggtgcgccgc
     2161 gcgctcaccg aggcgcaaac ctgagcgacc ggcgatgagg ccgtgaccgt cgcggccgcg
     2221 ctcctcgccg ccggacgcgg atcccgcctc ggcgccgacg ccaccgccaa acccctcctc
     2281 gagctgtccg ggcgtcgcct cgtcgactgg gccctcgacg ccgcgatcac cgccgggacc
     2341 cgcccggtgc tcctcgtcgt cggccacggc cgcgacgccg tcgccgcggc ggcaccgccc
     2401 acggtccagg tcatcaccgc caccgactgg ggtctcggta tcgcccattc ccttcgcgcc
     2461 gcgctcgacg cgctcgaacc ccaccccgag atcgacgcgg tctgcgtcgg actcgccgac
     2521 caacctcggg tcggccccga cgcctaccgg cggctcgtcg atgcccacca gcacggcgcc
     2581 accctcgctg tcgccaccta caccggtcag cgcgccaatc cggtgctcct cgaccggtcc
     2641 ctctggcccg aagcccggac cttgcacggc gacaccggcg cccgcgcgct catgagcacg
     2701 cacaccatca ccgaagtgga ctgcaccggc accggcgatc caaccgacgt cgatacgctc
     2761 gacgacctgc gcgccctcga ggggagaacc gaatgaagct cgagcacaac ttccgggtcg
     2821 gcgtcccggt ggacgaggcc tggacgacac tcctcgacct cgaacggatc gcgccctgca
     2881 tgcccggagc tcagctccaa gaagtcgacg gcgacgagta ccgcggcatc gtcaaggtca
     2941 aggtcggccc catcaccgcg cagtacaaag gcatcgcccg tctcaccgag gccgacgagg
     3001 cggcgggacg gctgatcatc accgccgagg gtcgcgacac ccgcggtcag ggcaacgcct
     3061 ccgcgacgat caccgcgcgt ctcagcgccg acggcgacgg cacccaggtc gccgtcgaca
     3121 ccgacctcaa cgtcaccggc aaagtggccc agttcggtcg cggggtcatg gccgacgtct
     3181 cgtcgaagct gctgggccag ttcgtcgact gtctgagcac cagcatcctc ggacctgggg
     3241 ctcccaccac ctccaccccg cccactcccg ccgcggcgcc cgcaccgggc ccgtcaagca
     3301 cccccggggc ggcacccgcc agctcggcgt cgccggcctc cccagcgtcg cccgggccgc
     3361 gacgcatcga cgcgcccgaa gccgaggccg tcgacctcat gcgggccgcc gggggagcag
     3421 tggcgaagcg cctcgtcccg ctcatcgccg ccggcgtcgt cgtggtcggg gtcgcggtgt
     3481 ggttcctcct ccgctgaaga tccgccaccg acgcacccct cgcccctagc atcgaataca
     3541 tgttcgatcg cgacggcccg gtcctcggtg tcgacccggg cctctcccag tgcggctacg
     3601 gcgtggtccg ccgcgacggc cggcggctcg cggccgtcgc ctgcgggatc ctgcgcaccg
     3661 accctggggc tccgctgccc gaacggctcg ccaccctcga ggccgacctc gacgagttcc
     3721 tccgggaagt agggccgtgc gccctcgcgg tggagcgagt cctgttccaa gccaacgtgc
     3781 gcaccgcgat ctcggtcgga caggccagcg gcctcgcgct cgcggtcgcg gcccgagccg
     3841 gggtccccgt gacccactac agcccgaacg aggtgaagct ggcgatcgcc gcgaacggcg
     3901 ccgctggcaa gagccaggtg caggcgatgg tgacccgggt gctgcgtctc tccgaacagc
     3961 caccaccgga cgccgccgac gccctcgcgc tcgcggtgtg ccatctttgg cgcatgccgc
     4021 tgcgcgcatc cgtggcccgt gctgtcgccg cagacgggga ccgccgctcg tgatcggatc
     4081 gctgcgcggc accgtgctgg aacgcaccgc gaccggtgag gcgatcgtcg aggtcggcgg
     4141 c
//
LOCUS       DATCCH010000399         2694 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_234326, whole genome shotgun sequence.
ACCESSION   DATCCH010000399 DATCCH010000000
VERSION     DATCCH010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234326"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /locus_tag="VIJ44_07695"
     CDS             complement(<1..814)
                     /locus_tag="VIJ44_07695"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5165817.1"
                     /translation="MEQLDGKVAVITGAARGIGEAIATRFAAEGMRLVLADVDPGALG
                     DAVAHHHANGTEVLGVPTDVSDAGAVDALAAAARERFGTSHVICNNAGVSGHARPSWD
                     QPEREWQWVLGVNLLGVVNGIRSFVPTLLAQDEGHVVNTASLAGFLAAPHLSPYAATK
                     HAVVALSTSLFHELAMTGSQVGVSVLCPSFLRTEIMDTDRAWPEGLGTSPGHADDPGS
                     ELLHGVFEHAVDEGLDPADLADRVVDGIRTRRFLVTTHQEQCVRAVQMTARVV"
     gene            1055..1546
                     /locus_tag="VIJ44_07700"
     CDS             1055..1546
                     /locus_tag="VIJ44_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165818.1"
                     /translation="MAHRATPPPVSGARRGMTIGLVALGVAIVGLIVAIAIVASSGGG
                     SSHAATTTTTVPATTTTPAISADPQGYAVALYNAWKTGDSVAASRVASPQAVTQMFAV
                     PYQAQASTTGPVDPYSFEGCQGAAGSTICSWQAQGLNTITMRVRNTTGGLPVLVVDIQ
                     RSP"
     gene            complement(1615..2583)
                     /locus_tag="VIJ44_07705"
     CDS             complement(1615..2583)
                     /locus_tag="VIJ44_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEY5165819.1"
                     /translation="MGIAKVWVYAETDGAKPTSSTLELLTKARELGDTVEAVYIGADA
                     DTLAGPLGEHGATTVYAVDPGDGLPGVIGAAALKELVATHQPDLIVFAQSYDGRDAVA
                     RLSAVLDRPVITNGTAVSVDGDRVIVGTAAFGGNTLVDTAFTGSAPYLAAIRPKSFPA
                     EPSGGGPATVEKVAAADAGRAGAAKVLERHVEEREGPKLEEATVVVSGGRGLGSAEAY
                     APLVEELAKLLHGASGASRAIVDAGWVPYAKQVGQTGKVVKPKLYVALGISGATQHLV
                     GMKGSDNILAINKDGEAPIFSVADLGVVGDVHKVVPKLIEAIKAKQ"
BASE COUNT          370 a          968 c          947 g          409 t
ORIGIN      
        1 ccacgacccg ggccgtcatc tggaccgccc gcacgcactg ctcctggtgg gtcgtgacga
       61 ggaaccgccg ggtgcggatc ccgtcgacga cccggtccgc gaggtccgcg gggtcgaggc
      121 cctcgtcgac ggcgtgctcg aacaccccgt gcaggagctc gctgcccgga tcgtcggcat
      181 gaccaggcga cgtgcccaga ccctcgggcc acgcccggtc ggtgtccatg atctcggtgc
      241 gaagaaacga cgggcacagc accgacacac cgacctggga gccggtcatc gccagctcgt
      301 ggaacagcga cgtcgacagc gcgacgacgg cgtgcttcgt cgccgcgtac ggcgacaggt
      361 gcggggccgc gaggaacccg gcgagcgacg cggtgttcac gacgtggccc tcgtcctgag
      421 cgagcaacgt cggcacgaag gaacggatcc cgttgaccac gccgaggagg ttcacgccga
      481 ggacccactg ccattcacgc tcgggttgat cccagctcgg ccgagcgtga ccggagacac
      541 cggcgttgtt gcagatgaca tgggacgtgc cgaaccgctc ccgggccgcg gccgcgagcg
      601 cgtcgacagc accggcgtcg gagacatcgg tggggacccc aagcacctcg gtgccgttgg
      661 cgtggtggtg ggcgacagcg tcgccgaggg cgccgggatc gacgtcggcc agcaccagcc
      721 gcatcccctc ggcggcgaag cgcgtcgcga tcgcctcgcc gatccctcgg gccgcgcccg
      781 tgatcaccgc gaccttgccg tccagctgct ccacgttgac ccctgtgtgt tgggttcgcc
      841 ccggcgctgg gtccggccgg tcaagcaccg gaccggtcct gccctgcccc gaccgctcga
      901 cgacacccgg gctcgatcct cccacgcccg gtcactcgct gcctccgagg gtgacagacc
      961 cgcgcccaag ccgggagcca tcgatgattg aaccctgccc tcggcgcgct gcggggtgtc
     1021 ccgatacggg cgggccggtc ggtagcgtcc agttgtggcc caccgcgcga ccccgccgcc
     1081 ggtgtccggc gcccgccggg gcatgacgat cgggctcgtc gccttgggcg tggcgatcgt
     1141 cgggttgatc gtcgcgatcg cgatcgtcgc ctcgtccggc ggcggaagct cgcacgccgc
     1201 gacgacgacc acgacggtcc ctgccacgac cacgaccccg gcgatcagcg ctgaccccca
     1261 gggctacgcc gtcgcgctgt acaacgcctg gaagaccggt gacagcgtcg cggcgtcacg
     1321 ggtcgcgtcg ccccaggccg tcacccagat gttcgcggtg ccgtaccagg cgcaggcgag
     1381 cacgaccggg ccggtcgatc cctactcgtt tgaaggctgc cagggtgccg cggggtcaac
     1441 gatctgctcg tggcaggccc agggcttgaa cacgatcacg atgcgggtgc gcaacacgac
     1501 cggtgggctg ccggtcctgg tggtcgacat ccagcgcagc ccctgatcgg gcacgctggg
     1561 ttttgcccgg tgttcgccgc ggcaggcgcc gcgcgaggtc accggccatg ggcgctactg
     1621 cttggctttg atcgcctcga tgagcttcgg gacgaccttg tggacgtcgc cgacgacgcc
     1681 gaggtcggcg accgagaaga ttggcgcctc gccgtccttg ttgatggcga gaatgttgtc
     1741 ggagcccttc atcccgacga ggtgctgggt cgcccctgag attcccagcg cgacgtacag
     1801 cttcggcttg acgaccttgc cggtctggcc gacctgcttg gcgtagggca cccagccggc
     1861 gtcgacgatg gcacgcgacg cgccggatgc cccgtgcagg agcttggcga gctcctcgac
     1921 gagcggcgcg taggcctcgg cgctgccgag accacgaccg cccgacacta cgaccgtcgc
     1981 ctcttcgagc ttgggtccct cacgttcctc gacgtggcgt tcgagcacct tcgcggcgcc
     2041 ggcccggccg gcatcggcgg ctgcgacctt ctccaccgtc gccggaccac ctccgctggg
     2101 ttcggcggga aacgacttcg ggcggatcgc ggcgaggtac ggcgccgacc cggtgaacgc
     2161 ggtgtcgacc agggtgttgc caccgaacgc ggcggtgccg acgatgaccc ggtcaccgtc
     2221 gacgctcacg gcggtgccgt tggtgatcac cggccggtcg agcaccgcgg agaggcgggc
     2281 gaccgcgtcg cgcccgtcgt agctctgcgc gaacacgatg aggtccggct ggtgggtggc
     2341 gaccagctcc ttgagcgcgg cggcgccgat gacgccaggg agcccgtcgc cgggatcgac
     2401 ggcgtacacg gtcgtcgccc cgtgctctcc gagcgggccc gcgagcgtgt cggcgtcggc
     2461 gccgatgtac accgcctcga ccgtgtcgcc gagctcccga gccttcgtga ggagctcaag
     2521 cgtgctcgag gtgggtttcg cgccgtcggt ctcggcgtac acccaaactt tggcgattcc
     2581 catcgtcctg cccctcagat gactttgagc gcttcgagga acgcgacgac gcgttcgtgg
     2641 gcgttgccgt cgtcctcgat cttctcgccg gcctcgcgtg ccggtgcggc cgcg
//
LOCUS       DATCCH010000400         2316 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_148146, whole genome shotgun sequence.
ACCESSION   DATCCH010000400 DATCCH010000000
VERSION     DATCCH010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148146"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..1210)
                     /locus_tag="VIJ44_07710"
     CDS             complement(29..1210)
                     /locus_tag="VIJ44_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009153707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-transfer protein"
                     /protein_id="HEY5165820.1"
                     /translation="MSDSNQLRDAGAVVGIGATEFSKDSGRSEMRLAVEACEAAIADA
                     GLEPRQIDGMVTFTADTNPEIEIARNLGIGELSFFSRIHHGGGAACATIQQAVLAVNA
                     GVADYVVCYRAFNERSGHRFGTGVQGRPALATADSAHFAWYAPFGLLTPAQWVAMLAQ
                     RYLHEYGATSEDFGRIAVADRKHAAANPAAWFYGQPITLEDHQASRWIVEPLHLLDCC
                     QESDGGQALVVTTPERARDLAQPPVIVRAAAQGAGENQDMMTSYYRDSITGLPEMGLV
                     ARQLWNTSGLGPDDIQAAIIYDHFTPLALPQLEEFGFCGRGEAKDFIRDGNIELGGRL
                     PINTNGGQLGEAYIHGMNGIAEGARLIRGTSTNQPAGVEHVLVTAGTMVPTSALLLGA
                     A"
     gene            complement(1207..1623)
                     /locus_tag="VIJ44_07715"
     CDS             complement(1207..1623)
                     /locus_tag="VIJ44_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019807666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEY5165821.1"
                     /translation="MTTTTSLRAADVSVGDQLPGLDIPLTRTLIVSTAIASRDYQDVH
                     HDPGLAQERGSKDIFMNILSTNGFVGRYITDWAGPDALLKAVKIRLGAPNYPGDTMVM
                     TGTVTKKDGSVVEVSLRGANSLGDHVTGTVTLELRP"
     gene            complement(1620..>2316)
                     /locus_tag="VIJ44_07720"
     CDS             complement(1620..>2316)
                     /locus_tag="VIJ44_07720"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEY5165822.1"
                     /translation="ATRDGAGWRLDGFKPSVPAAHVAQQVLVPARTDDAVTVFLLDPH
                     TSGVTIERATTTNREIHPHLHLDGAVAEPLGAPDAGNRVLSWMLDRALIGLCGLQVGI
                     CEAALALAAGYSSNRVQFGKPLSTFQGVALKAADGYIQTEGIRVTMLQAAWRLDAGRE
                     ASSDVLVAKWWASEGGQRVVHIAQHLHGGMGADVDYPVHRYFLWGKQIEDTLGGSSAQ
                     LARLGEALAGAPR"
BASE COUNT          308 a          741 c          883 g          384 t
ORIGIN      
        1 ggggtggcgg ggtcgccgag gggattggtc acgccgcccc gagcagcagc gcgctggttg
       61 gcaccatggt cccggcggtg acgagcacgt gctcgacccc ggcgggctga ttggtggacg
      121 tccctcgaat cagcctcgcc ccttcggcga tgccgttcat cccgtggatg taggcctcgc
      181 ccagttgccc accgttggtg ttgatgggca ggcgaccgcc gagctcgatg ttgccgtccc
      241 ggatgaagtc cttggcctcc ccccggccgc agaacccgaa ctcctcaagc tgcggcaacg
      301 cgagcggggt gaagtggtcg tagatgatcg cggcctggat gtcgtcgggt ccgaggccgc
      361 tggtgttcca gagctggcgg gccacgaggc ccatctcggg caacccggtg atggagtccc
      421 ggtagtagct ggtcatcatg tcctggttct cgcccgcgcc ttgcgccgcg gcgcggacga
      481 tcactggcgg ttgggccagg tcgcgggccc gctcgggtgt ggtcacgacg agggcttggc
      541 cgccgtcgct ctcctggcag cagtcgagga ggtgtagcgg ctccacgatc cagcgggacg
      601 cctggtggtc ctcgagcgtg atgggctggc cgtagaacca ggccgcgggg ttcgccgcgg
      661 cgtgcttgcg gtccgcgacc gcgatgcgcc cgaagtcttc gctggtggcg ccgtactcgt
      721 ggaggtagcg ctgggcgagc atcgccaccc actgcgccgg agtcaggagc ccgaacggcg
      781 cgtaccaggc gaagtgcgcc gagtccgcgg tggcgagcgc tgggcggccc tgcacgccgg
      841 tcccgaagcg gtgcccggac cgttcgttga aagcccggta gcagaccacg tagtcggcga
      901 cgccggcgtt gaccgcgagg accgcctgtt ggatcgtcgc gcaggcagcg ccgccgccgt
      961 ggtggatgcg ggagaagaag gagagctcac cgatgccgag gttgcgggcg atctcgatct
     1021 cggggttggt gtcggcggtg aaggtcacca tcccgtcgat ctggcgcggc tcgagacccg
     1081 cgtcggcgat ggcggcttca caggcctcga ccgccaggcg catctcggag cgcccggagt
     1141 ccttggagaa ctcggtggcg ccgatgccga cgacggcgcc ggcgtcgcgc agctggttcg
     1201 agtcgctcac gggcgaagct ccagtgtgac cgtcccggtc acgtggtccc cgaggctgtt
     1261 cgcgccgcgc agtgacacct cgacgacgga gccgtccttc ttggtgacgg tcccggtcat
     1321 gaccatggtg tcgccggggt agttgggggc accgagccgg atcttcacgg ccttgaggag
     1381 cgcgtcgggg ccggcccagt cggtgatgta gcggccgacg aagccgttgg tgctgaggat
     1441 gttcatgaag atgtccttcg atccgcgttc ttgggcgagg ccgggatcgt ggtggacgtc
     1501 ttggtagtca cgggacgcga tcgcggtgga cacgatcagc gtccgggtca acgggatgtc
     1561 gagccccggg agttggtcgc cgacgctgac gtcggcggcg cgaagggagg tggtggtcgt
     1621 catcgtggcg ctcctgctag tgcttcgccg agacgagcga gctgggcgct cgacccgccg
     1681 agcgtgtctt cgatctgctt gccccacagg aagtagcggt ggaccgggta gtcgacgtcg
     1741 gcgcccatcc cgccgtggag gtgctgggcg atgtgcacga cccgctgccc accttccgag
     1801 gcccaccact tggcgaccag gacgtcgctg gacgcctctc ggccggcgtc gaggcgccat
     1861 gcggcctgga gcatcgtgac gcggatgcct tcggtctgga tgtagccgtc cgcggctttg
     1921 agggccacgc cttggaaggt ggagagcggc ttcccgaact ggactcggtt cgagctgtac
     1981 ccggcggcga gcgccagcgc ggcctcgcag atccccacct ggaggccgca cagcccgatc
     2041 agcgcccggt ccagcatcca ggagaggact cggttcccgg cgtcgggcgc gccgaggggc
     2101 tcggcgacgg ccccgtcaag gtgcaggtgc gggtggatct cccggttggt cgtcgtggct
     2161 cgctcgatgg tgacgcccga cgtgtgcggg tcgagcagga acaccgtgac cgcgtcgtcg
     2221 gtgcgggcgg gcaccaggac ctgttgcgcg acgtgcgccg cggggactga cggcttgaac
     2281 ccgtcgagtc gccacccggc gccgtcgcgg gtggcg
//
LOCUS       DATCCH010000401         2916 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_608960, whole genome shotgun sequence.
ACCESSION   DATCCH010000401 DATCCH010000000
VERSION     DATCCH010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608960"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..1157)
                     /locus_tag="VIJ44_07725"
     CDS             complement(42..1157)
                     /locus_tag="VIJ44_07725"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEY5165823.1"
                     /translation="MTAGPESPIRVAVLADTVTGRAGMGRYAREVLRGLARREDVALV
                     PIVPRVAAGAIEALDLARPVLDQIEVPGRTRVEQGLWERYRLGTVLERHPVDVVHGIK
                     HIVPRTNRPTVLTVHDLVTLTWPQQADLVRRLLLPRQYRASLRSAQVLVTYSEATRRR
                     LADFDPALGAKAVTAPIGFTPELLSADPLPVPDLVGRTFALVVGDLSPRKNIGFLVEL
                     WPEIFAATGGLLLAVVGPDGARSRSTRHRVAELVEAGMAVRPGRVAEGALRWCYEHAR
                     VVLLPTLEEGFGLPVLEAAVFQVPVVANPEPALVEVGQGWPTFVDLNDRARWIEAIVE
                     TSPTGETRHSPLTAWPTWDEHVSGLVGAYRLAIRATT"
     gene            complement(1154..2341)
                     /locus_tag="VIJ44_07730"
     CDS             complement(1154..2341)
                     /locus_tag="VIJ44_07730"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY5165824.1"
                     /translation="MMLGNPTRVLMVSSLWPPAVLGGAETYAAGLAERLRSAGHEVGV
                     VTLGVPGADVVGEVPPCPYRLDEFASQPRWRRAIFHALDLYRPATRRVIAEACQRFQP
                     DVVHTHCVQGLSSLALEIPSRLGIGHVHTLHDYWLLCQRASLVKRDGTACEERCRSCR
                     VISSSRNWQIARHPPDVVIAVSEAIAAEHRQLPWVEPRLRVIHNPIDPEPHARPGTHD
                     PLTFGYLGQVTKTKGVATLVEAFTRADLPRARLVIAGDGSLRAELQAAGLHNVELPGW
                     VDAADREAFFAETDCLVVPSEWKDPAPLVVNEARAHGIPVIGARIGGIPELVAPASQP
                     LLFRSGDAAELGVRLAEFASAPDRYRGDRDDAPGWPEHLQAIQDAYERARSTAVTRAA
                     EKQ"
     gene            complement(2338..>2916)
                     /locus_tag="VIJ44_07735"
     CDS             complement(2338..>2916)
                     /locus_tag="VIJ44_07735"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY5165825.1"
                     /translation="AARTRLGLRGAPLAVCVGRLVFQKGQDILVSAWPEVRRRVPDAQ
                     LAVIGEGETHAELAGLSTPGVTIVGPVADVRPWLVAADIVVQPSRWEGRSSSVLEALA
                     SGRSVVATDVEGMREAIGEDPVTRAGAIVPPNDPDALAAAIAARLANSNVVEAEARYD
                     ASRVARFGLEPWREAIATVTRDALNRSGDSSR"
BASE COUNT          455 a          993 c         1049 g          419 t
ORIGIN      
        1 gaaccggcgg tcgtgtcgct cctcgtcggg gcgcgtgccg gtcaggtcgt ggcgcggatg
       61 gcgagccgat aggccccgac gagccccgag acgtgttcgt cccaggtggg ccacgccgtc
      121 agcggcgaat gccgggtctc cccggttggg ctggtttcga cgatggcctc gatccaccgg
      181 gcccggtcgt tgagatccac gaacgtcggc catccctgcc cgacctcgac gagcgccggc
      241 tctgggttgg cgaccacggg cacctgaaag accgcggcct cgaggacggg gagcccgaaa
      301 ccttcctcca aggtggggag cagcacgacg cgcgcgtgct cgtagcacca ccgcaacgca
      361 ccctcggcga cgcgcccggg ccggaccgcc atgccggcct cgaccagctc cgcgacgcgg
      421 tgtcgtgtcg agcggctcct ggcgccgtcc gggccgacga ccgcaaggag gagtccaccc
      481 gtcgcggcga agatctcggg ccacagctcg acgaggaagc cgatgttctt gcggggcgaa
      541 agatcgccca ccacgagtgc gaacgttcgc ccaacgaggt caggcacggg cagcgggtcg
      601 gcggagagca gctcgggcgt gaacccgatc ggcgcagtga ccgccttggc tcccaaagcg
      661 gggtcgaagt cggcgagccg gcggcgggtc gcctcgctgt acgtgaccaa cacctgcgcg
      721 ctccggagcg aagcccggta ctggcgtggc agcaacagtc gccgaaccag gtcggcttgt
      781 tggggccagg tgagggtgac gagatcgtgg accgtgagca cggtcgggcg attcgttctc
      841 ggcacgatgt gcttgattcc gtgcaccacg tcgacggggt ggcgctcgag cacggttcca
      901 agccgatatc gctcccacag gccctgttcg acccgggtgc gccccgggac ctcgatctgg
      961 tcaagtactg gtcgggcgag gtcgagggcc tcgatcgcgc cggcggcgac ccgcgggacg
     1021 atcgggacca gggccacgtc ctcccgacgg gcgagacccc ggaggacctc tcgggcatat
     1081 cgacccatgc cggcgcgccc ggtgacggtg tcggccagca ccgcgactcg aatgggcgac
     1141 tcggggcccg cggtcactgt ttctccgcgg cgcgtgtcac ggccgtcgag cgggcgcgct
     1201 cgtaggcgtc ctgaatcgcc tgcagatgtt cgggccagcc aggtgcatcg tcacggtctc
     1261 cccggtagcg atcgggagcg gacgcgaact cggccagtcg aacaccgagc tcggcggcgt
     1321 cgccggaccg gaacagcagc ggctggctgg ccggtgcgac gagctcgggg atgccgccga
     1381 tgcgcgctcc gatgaccggg atcccgtggg cccgcgcttc gttgacgacg agcggcgcgg
     1441 ggtccttcca ttccgacggg acgaccaggc aatccgtttc ggcgaagaac gcctccctgt
     1501 cggccgcgtc gacccaaccc ggaagctcga cgttgtgaag gccggcggcc tgcagttccg
     1561 ctcggagaga gccgtcgccg gcgatgacga gacgagctcg tggaaggtca gcgcgagtga
     1621 acgcctcgac cagcgtcgcg acgcccttcg tcttcgtaac ctggccgagg tacccgaagg
     1681 tcagcgggtc atgggtaccc ggtcgggcgt ggggttccgg gtcgatcggg ttgtggatga
     1741 cgcggaggcg aggctcgacc catggcagtt ggcgatgctc agcggcgatg gcctccgaga
     1801 cggcgatcac aacgtcaggt ggatgccgcg cgatctgcca gttccggctg gatgagatga
     1861 cgcggcacga gcggcatcgc tcttcgcacg ccgtgccgtc gcgtttcacc agcgacgcgc
     1921 gctggcagag cagccagtag tcgtgaagcg tgtggacatg cccgatcccg aggcgggacg
     1981 gaatctcaag cgccaggctt gacagccctt ggacgcaatg agtgtggacg acgtcaggtt
     2041 ggaaccgctg acaggcctcg gcgattaccc ggcgtgtcgc gggtcggtac agatcgagcg
     2101 cgtggaagat ggcacgacgc cagcgtggct gcgaggcgaa ctcgtcgaga cggtacgggc
     2161 acggcggcac ctcaccgacc acgtccgcgc ccggaacccc gagcgtgacc acgccgacct
     2221 cgtgtccagc cgagcgcagt cgttcggcga gcccggcggc gtacgtttcg gcgccgccca
     2281 acaccgcggg gggccagaga ctcgacacca tgagcactcg ggtcgggttg cccagcatca
     2341 tcgagacgag tcacccgacc tgttcagcgc gtctcgcgtc accgtggcga tcgcctcgcg
     2401 ccacggctcg agtccgaatc gcgccacacg ggacgcgtcg tatcgggcct cggcctcgac
     2461 gacgttggag ttggcgagcc gcgccgcgat tgctgccgcg agcgcgtccg gatcgttggg
     2521 cggcacgatc gcgccggctc gggtcaccgg gtcctcacca atcgcctcgc gcatgccttc
     2581 gacatcggtt gcgacgacgc tgcgaccgga ggcgagcgcc tcaagcaccg aggacgaccg
     2641 cccttcccat ctggacggct ggacgacgat gtcggcggcg acgagccagg gacggacatc
     2701 ggcgacgggg ccgacgatgg tcaccccggg cgtcgacagc ccggcgagct ccgcatgcgt
     2761 ctctccttcg ccgatcaccg cgagttgggc gtcggggact cggcgacgca cctcgggcca
     2821 tgcgctgacg agtatgtctt ggcccttctg gaacaccagc cggccgacgc acaccgcaag
     2881 cggcgcgccc ctgagtccca atcgggtccg tgcggc
//
LOCUS       DATCCH010000402         3570 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_443592, whole genome shotgun sequence.
ACCESSION   DATCCH010000402 DATCCH010000000
VERSION     DATCCH010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3570
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443592"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..1239
                     /locus_tag="VIJ44_07740"
     CDS             94..1239
                     /locus_tag="VIJ44_07740"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HEY5165826.1"
                     /translation="MTAFAELVAGRGVVLCCGTGGVGKTTTAAVISLIAARQGRNAVV
                     VTIDPAKRLADTLGLAELGNTAREIPRERWDLDGDAAPGGHLSALMLDTRATFDGLVR
                     RYAQDGAQATRILENRFYRNIAGALSGTQEYMAMEKLHELYDEGGYDLIVVDTPPSRH
                     ALDFLDAPQRLTRLLDNRIFRLLMMPTRTYLRLAGAAVQRFLRTVGKVLGTEVLDDIV
                     AFFRAFEGMEQGFRDRAARVDELLAEPQTAFVLITSPRRDAVDEAEFFAAQLREANIP
                     IDALIVNRVEPDFGSSASVSSLEDRAEALDAAPSDAAHRLAARFRNLADYVDLGQRER
                     YDLADVGQRIGAGTIAYVPSLPHDVVDFSALDAVARHLFGDPTHGGE"
     gene            1249..1629
                     /locus_tag="VIJ44_07745"
     CDS             1249..1629
                     /locus_tag="VIJ44_07745"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY5165827.1"
                     /translation="MATVLVAADGAWIRDQVRTALTGPDLELIEVNRGQEVRDVVARG
                     HADLVILDLQIANMGGIAVAIDLHLEASAARLPNVPILLLLDREHDRFLAKRAAADAE
                     LVKPIDPGTLRRTVKQLLGQRAAV"
     gene            1703..1779
                     /locus_tag="VIJ44_07750"
     tRNA            1703..1779
                     /locus_tag="VIJ44_07750"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1737..1739,aa:Pro,seq:cgg)
     gene            complement(1860..2891)
                     /locus_tag="VIJ44_07755"
     CDS             complement(1860..2891)
                     /locus_tag="VIJ44_07755"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018653766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEY5165828.1"
                     /translation="MRVGVVGATGLVGHEMLRVLEERKFPVGELRVFASPRSEGRRLH
                     FGDDDVVCQRLADGCFDGLDLVIVDVDDPLAAAWAPQAVAAGAKVVDNSAAFRMDPDV
                     PLVVAEVNPSDARTLPKGIVSCPNCTTMILVTALAPLHRAATIDRLVVSTYQSVSGAG
                     QSGMRELAEQWTKGAGQEETLRRAGAIPGAITPGEVWSRPVAGNVIPLAGSLEDRGYT
                     SEEWKLVRESRKILHAPDLNVSPTCVRVPVYVGHSMSANVRFVHGLSRADAAAVLADA
                     SGVTLVDGGDGAPTPLEGAGVDPVLVGRLRDDPFEERTLNLWVTGDNLRKGAALNAVQ
                     LAELLAAPN"
     gene            complement(2895..>3570)
                     /locus_tag="VIJ44_07760"
     CDS             complement(2895..>3570)
                     /locus_tag="VIJ44_07760"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012784200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEY5165829.1"
                     /translation="DPRIVPKARRLPRVSYEEMLDLSATGGRVLALRSVEFARNYHVP
                     VHVRSSFTWEPGTWVGEEDPSMEQAIISGVTHDVSEAKVTIARVPDRPGIAARLFRTL
                     ADESVNVDMIVQNVSSAGHTDISFTVPKDDLTRTLKVMETIVDDLEASGVTHDADVGR
                     VSIVGAGMKTNPGIAASMFEVLAGDDINIEMISTSSIRISCVVQAADVERAVRALHHA
                     FQLERG"
BASE COUNT          547 a         1235 c         1224 g          564 t
ORIGIN      
        1 gccgttcgtc ttcggggaga tcggaccggc ccacgtggag gtgctcgccg acgcgctcac
       61 cgcgggcatc gtcgcgctcc ccgcaccacc gtcgtgacgg cattcgccga gctggtcgcc
      121 gggcggggcg tggtgttgtg ctgcggaacc ggtggcgtcg gcaagaccac cactgcggcc
      181 gtcatctcgc tcatcgcggc acgacagggc cgcaacgcgg tggtggtgac gatcgacccg
      241 gcgaagcgtc tcgccgacac gctcgggctc gccgagctcg ggaacactgc tcgcgagatc
      301 ccccgtgagc gatgggacct cgatggtgac gcggccccgg gcgggcacct ctcggcgctg
      361 atgctcgaca cccgggccac gttcgacggg ctcgtccgcc ggtacgccca ggacggggcg
      421 caagccaccc gcatcctcga gaaccgcttc taccgcaaca tcgccggcgc gctctcgggc
      481 acccaggagt acatggcgat ggagaagctg cacgagctct acgacgaggg tggctatgac
      541 ctcatcgtcg tcgacacccc accttctcgg catgccctcg acttccttga cgccccgcaa
      601 cgcctgaccc ggctgctcga taaccgcatc tttcgattgc tgatgatgcc aacccggacc
      661 taccttcggc ttgcgggcgc ggccgtgcaa cgcttcctcc ggaccgtcgg gaaggtcctc
      721 ggcaccgagg tcctcgatga catcgtcgcc ttcttccggg cgttcgaagg aatggagcag
      781 ggcttccgtg atcgggcggc ccgggtcgac gagttgctcg ccgagccgca gaccgcgttc
      841 gtgctcatca cgtcacctcg gcgtgacgcc gtcgacgagg cagagttctt cgcggcccag
      901 ctccgcgaag ccaacatccc gatcgatgcg ctcatcgtca accgggtcga acccgacttc
      961 ggttcctcgg cgtccgtgag ttccttggag gaccgcgccg aggcgctcga cgcggcgccg
     1021 agtgacgcgg ctcaccgcct cgcggcgcgg ttccggaacc tcgccgacta cgtcgacctc
     1081 gggcagcgag agcgatacga cctcgccgac gtcgggcagc ggatcggggc aggaacgatc
     1141 gcctacgtgc cgtcccttcc ccacgacgtc gtcgacttct cggccctcga cgcggtcgcc
     1201 cgccacctct tcggcgaccc gacacacggt ggcgagtagg ttccccccgt ggccactgtc
     1261 ctcgttgccg ctgacggcgc ctggatccgc gatcaggtgc gcacggccct gaccggaccc
     1321 gatctcgagc tcatcgaggt caaccgcggc caggaggtac gcgacgtcgt cgcccgcggc
     1381 catgccgatc tggtcatcct cgacctccag atcgccaaca tgggcggcat cgcggtcgcc
     1441 atcgacctgc atctcgaggc atccgcggcg cggctcccga acgtgccgat cctgttgctc
     1501 ctcgaccgtg agcacgaccg gttcctcgcg aaacgggccg ccgcggacgc cgagttggtg
     1561 aaacccatcg atcccgggac gctgcggcgc accgtcaagc agctcctcgg tcagcgcgcc
     1621 gccgtctagt cgcgccagcg atcggtcggc gtccgcgccc ggcggggcga cacgggcccg
     1681 ccactacact cacctcttct tacgggctgt ggcgcagttt ggtagcgcgc ttcgttcggg
     1741 acgaagaggt cccgggttca agtcccggca gcccgaccat cccctccccg ttggcctcac
     1801 gcctggtgct cgcatcgggt cctggaccgt gtcacggatc gaaccggcgg caatgccgcc
     1861 tagttcggcg cggcgagcag ctcggcgagc tgcaccgcgt tgagcgcggc gcctttgcgc
     1921 aggttgtccc cggtgaccca gaggttgagg gtccgttcct cgaacgggtc gtctcggagc
     1981 cgtccaacga ggacggggtc gactccggcc ccttcgagag gagtgggcgc gccgtctcca
     2041 ccgtcgacga gggtcacgcc ggacgcgtcg gcgaggacgg cggcggcgtc ggcacgtgag
     2101 agtccgtgga cgaatcggac gttggcgctc atggagtgcc cgacgtagac ggggacacgg
     2161 acacaggtcg ggctgacgtt gaggtcgggc gcgtggagga tcttgcggct ctcgcgcacc
     2221 agcttccatt cctcggatgt gtagccccgg tcctcgaggg accccgcgag ggggatgacg
     2281 ttccccgcga cggggcggga ccagacctca cccggggtga tggcgccggg gattgcgccg
     2341 gcgcgccgca gggtctcttc ttgaccggcg cctttcgtcc actgctcggc gagctctcgc
     2401 atcccgctct gtcccgcgcc ggacaccgac tggtaggtgg agacgacgag ccggtcgatc
     2461 gtcgcggcgc gatgcagggg cgccagcgcg gtgacgagga tcatcgtggt gcagttggga
     2521 caggacacga tgcccttagg gagcgtgcgg gcgtcgctcg ggttgacctc ggcaacgacg
     2581 agaggcacgt cgggatccat gcggaacgcg gccgagttgt ccaccacctt cgcgcccgcg
     2641 gcgacggcct gcggggccca cgcagctgcg agggggtcat cgacgtcgac gatcacgagg
     2701 tcgaggccgt cgaagcagcc gtcggcgagg cgctgacaga ccacgtcgtc gtccccgaag
     2761 tgaagacggc gaccttcgga acggggcgag gcgaagaccc gaagctcccc gacagggaac
     2821 ttgcgttcct cgaggactcg cagcatctcg tgaccgacca ggccggtggc gccgaccact
     2881 ccgactcgca cgcctcagcc ccgctcgagt tggaaggcgt ggtgcagggc ccgcacggcg
     2941 cgttcgacat cggcggcctg gacaacacag gagatgcgga tcgacgaggt ggagatcatc
     3001 tcaatgttga tgtcgtcgcc ggcgaggacc tcgaacatcg aggccgcgat gcccgggttg
     3061 gtcttcatgc cggcaccgac gatggagacc cggccgacgt cggcgtcatg agtcacgccg
     3121 ctggcctcga ggtcgtcgac gatggtctcc atgaccttca acgtccgggt caggtcgtcc
     3181 ttggggaccg tgaacgagat gtcggtgtga ccggcgctcg acacgttctg cacgatcatg
     3241 tcgacgttga cggattcgtc cgcgagggtt cggaacaagc gggcggcgat cccgggacgg
     3301 tccgggacac gcgcgatcgt gaccttcgcc tccgagacgt cgtgcgtgac gccagagatg
     3361 atcgcctgct ccatggacgg gtcctcctct ccgacccacg tgcccggctc ccaggtgaag
     3421 ctggaacgga cgtggaccgg gacgtgatag ttgcgagcga actcgaccga gcgcagcgca
     3481 aggacccgac cgccggtcgc agagaggtcg agcatctcct cgtaggacac ccggggcagt
     3541 cgacgagcct tcgggacgat gcggggatcc
//
LOCUS       DATCCH010000403         2297 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_357718, whole genome shotgun sequence.
ACCESSION   DATCCH010000403 DATCCH010000000
VERSION     DATCCH010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357718"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="VIJ44_07765"
     CDS             complement(<1..908)
                     /locus_tag="VIJ44_07765"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEY5165830.1"
                     /translation="MVAGHAGIAEAGPSMQYVAEQFTPEEEAVLRPYVTNLDLPVFAL
                     VNLPEVVKGALFARYSRSPKSLRRLFVDEFVGDLDLTGDLTVDASVGLTRAEELYDRV
                     FLEYGDDSVAQLGGVHLACEQASNVLTKVLEWGRLMAYLEQSTRYIPYTARLAGRYRY
                     HRPPIILESALGLRFIADLDAAFDDYAACLPVLLEWARDRYPKEPGDSDFVYKQSLKA
                     KACDAARGILPAATLSNVGIYGTGQAFEALLLRMRAHPLPEARGYAAMMLDELRKVIP
                     SFLTRVDRPERGGAWSAYLAETRTDTA"
     gene            complement(1013..1660)
                     /locus_tag="VIJ44_07770"
     CDS             complement(1013..1660)
                     /locus_tag="VIJ44_07770"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165831.1"
                     /translation="MIRPGQPWGAPTAGPPDATGAGSDRDLAALADAHRGARLHFEPD
                     ERCEFARAVGLTPAGPGVTDLAVDALDVDDVGLAVNMVVAGVAPDRLGPWSRARPVTV
                     RVDGRPVHDGPATTVVVANGQFRRGVDLVPRGHPGDGRLEVQVYALRPGERAGMRRRL
                     PGGGHVPHPRIVTTTGRQVEIRWAAPAPLEVDGTSSPRRSSVEVTVRSPAFVLVV"
     gene            complement(1657..>2297)
                     /locus_tag="VIJ44_07775"
     CDS             complement(1657..>2297)
                     /locus_tag="VIJ44_07775"
                     /inference="COORDINATES: protein motif:HMM:NF019758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA helicase"
                     /protein_id="HEY5165832.1"
                     /translation="RIEREITRLERRVRGRSDSLGLQFDRVLGLLESWGYVDGWSLTP
                     PGEILARLYTETDLLLAEAVREGRLDGLTTPETAAVVSCFTYERRGSDDAATPPLRWP
                     TRRVAERSREIESLWQTLVKAEDDAGLPQTRPPDPGFTPYIYEWVLGESLAAVLDDEE
                     LAGGDFVRNVKQCIDLLRQVAEVAPEAATRDAARDAADAAFRGVVAAASTVP"
BASE COUNT          328 a          796 c          854 g          319 t
ORIGIN      
        1 gcggtgtcgg tccgggtctc ggccaagtag gcactccacg cgcccccccg ctccggacgg
       61 tcgacgcggg tgaggaacga cgggatcacc ttgcggagct cgtcgagcat catggccgcg
      121 tacccccggg cctcggggag cggatgggcc cgcatgcgca gcagcagcgc ctcgaacgcc
      181 tgcccggtgc cgtagatccc gacgttcgac aaggtggcgg cggggaggat gccccgtgcc
      241 gcgtcgcacg ccttcgcttt caacgactgc ttgtacacga agtcggaatc gcccggttct
      301 ttcgggtacc ggtcccgagc ccattcgagg agcacgggca gacaggccgc gtagtcgtcg
      361 aacgcggcgt cgagatcggc gatgaaacgc aaaccgagcg ccgactcgag gatgatcggc
      421 ggccggtggt accggtaccg gccggcgagg cgcgcggtgt acgggatgta tcgggtcgac
      481 tgctcgaggt aggccatgag ccggccccac tccaagacct tggtgagaac gttcgaggcc
      541 tgctcgcacg ccaggtgcac acccccgagc tgggcgaccg agtcgtcgcc atactcgagg
      601 aacacccggt catagagctc ctcggcccgg gtgagcccga cgctggcgtc gaccgtcagg
      661 tcaccggtga ggtcgaggtc accgacgaac tcgtccacga acaagcgacg cagactcttg
      721 gggctgcgcg agtaccgggc gaacaacgcc cccttgacca cctcgggcag gttcacgagc
      781 gcgaacaccg gcaggtccag gttcgtcacg tacgggcgca gcaccgcctc ttcctcgggc
      841 gtgaactgct cagcgacgta ctgcatgctc ggtcccgctt cggcgatccc ggcgtggccg
      901 gcgaccatgg aggagcgatc gtaccgacgg cctcccggag ccacgcggat gaggtgtttc
      961 gcggcctttg tcgggttcgc cggtcgctgg ggggtgtcgg gtcgggcgaa ggtcagacca
     1021 cgaggacgaa cgcggggctg cggacggtga cctccaccga ggatcgacgg gggctggacg
     1081 tcccgtcgac ctcgagcggc gccggtgccg cccagcggat ctcgacctgc cggcccgtgg
     1141 tggtgacgat gcgggggtgc gggacgtggc ccccgccggg gaggcgtcgc cgcatcccgg
     1201 cccgctcccc gggccggagg gcgtacacct ggacctcgag gcgcccgtcg ccggggtggc
     1261 ctcggggcac caggtcgacc ccgcggcgga actgcccgtt ggcgacgacg acggtggtgg
     1321 cgggcccatc gtggaccggc cggccgtcga cccgcaccgt gaccggccgg gcccgagacc
     1381 aggggccgag ccggtcggga gcgacgccgg cgacgaccat gttcacggcc aatccgacgt
     1441 cgtcgacgtc gagggcgtcg accgcgaggt cggtgacacc ggggccggcg ggggtcaatc
     1501 cgacggcccg ggcgaactcg cagcgctcgt cgggttcgaa gtggagtcgg gcgccgcgat
     1561 gcgcgtcggc gagtgctgcc aagtcccggt cgctgccggc gccggtcgcg tcgggcggcc
     1621 cggcggtcgg tgccccccac ggctgaccgg gccggatcac ggcaccgtgc tcgcggcggc
     1681 cacgacaccc cggaacgcgg cgtcggcggc gtcgcgtgcc gcgtcgcggg tcgccgcctc
     1741 aggtgccacc tcggcgacct ggcgcagcag gtcgatgcac tgcttgacgt tgcggacgaa
     1801 gtcgccgccg gcgagctcct cgtcgtcgag gaccgcggcg agggactcgc ccagcaccca
     1861 ctcgtagatg tagggcgtga agcccggatc cggaggacgg gtctggggca ggccggcgtc
     1921 gtcttcggct ttgaccaggg tctgccacag cgattcgatc tcgcgggacc gctccgcgac
     1981 gcgccgcgtc ggccatcgca gcggcggggt cgctgcgtcg tcggagccgc gccgctcgta
     2041 ggtgaagcag gacacgaccg ccgcggtctc cggtgtcgtc agcccgtcga ggcgtccctc
     2101 ccggaccgct tccgcgagca acaggtcggt ctcggtgtac aggcgggcca ggatttcacc
     2161 cggtggggtc agcgaccagc catcgacgta gccccacgat tcgagcagcc cgagcacccg
     2221 gtcgaattga aggccgaggc tgtcgcttcg gccccggacc cgtcgctcca ggcgggtgat
     2281 ctcacgctcg atgcgtt
//
LOCUS       DATCCH010000404         2018 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_24490, whole genome shotgun sequence.
ACCESSION   DATCCH010000404 DATCCH010000000
VERSION     DATCCH010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24490"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="VIJ44_07780"
     CDS             <1..239
                     /locus_tag="VIJ44_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009357672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY5165833.1"
                     /translation="AKTDGAPDDWYVIGDRCSHADYALSEGDVWTDEREIECPKHGST
                     FSLLTGEPLSLPATKPVPVFGVRVDGDDVYVELP"
     gene            236..1036
                     /gene="sufC"
                     /locus_tag="VIJ44_07785"
     CDS             236..1036
                     /gene="sufC"
                     /locus_tag="VIJ44_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEY5165834.1"
                     /translation="MTSHLAVRGLYASVNGVDVLRGVDLDIASGEVHALMGPNGSGKS
                     TLSHVLMGRADYEVTAGSITLDGTELLGMPTWRRAELGLFLAMQYPISVPGVPVGALL
                     GAAAAARGEAVDDLDARVHAEAGRLAIADEFLTRGVNDEFSGGEQKRMETMQLALLHP
                     KIAILDELDSGLDVDALRDIARRVAALTRDAALGVLAITHYARLLSELRPDRVHVLMA
                     GRIVTSGGPELADQLEAGGYEGLASELGVDELTIRATKPSDPFAEPGF"
     gene            1168..>2018
                     /locus_tag="VIJ44_07790"
     CDS             1168..>2018
                     /locus_tag="VIJ44_07790"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5165835.1"
                     /translation="MPASSPRSDFSVVADRLAFSRGGQVILDGVNVAIGPRTRLGIVG
                     PNGVGKSTLLRILGGLQEPDRGQVTRAPATLRVAYLDQERGRAHETVRAWFERRSGVA
                     AAAATFDAATEAVAARRPGADDAYAAALDAYLDGGCADFEARVATVVADLGLAPGILD
                     TSTDTLSGGEAARVALASLELVRADVLLLDEPTNDLDFDGLDRLERYLASLSGGVVVV
                     SHDRAFLERTVTSVGELDEHTHRAQVYAGGWHAYLEARGTARRHAEDDYATYRSERDR
                     LGRRAQR"
BASE COUNT          281 a          774 c          665 g          298 t
ORIGIN      
        1 cggccaagac cgatggcgcc cccgacgact ggtacgtgat cggcgaccgc tgctcccacg
       61 ccgactacgc cctctccgaa ggcgacgtct ggaccgacga acgcgagatc gagtgcccca
      121 agcacgggtc gacgttctcg ctgctcaccg gcgagcccct cagcctgccc gccaccaagc
      181 cggtgccggt cttcggcgtc cgcgtcgatg gcgacgatgt ctacgtggag ctcccgtgac
      241 gagtcacctc gccgtccgtg ggctgtacgc ctcggtcaac ggggtcgacg tgctgcgtgg
      301 tgtcgacctc gacatcgcct cgggcgaggt acacgccctc atgggcccga acgggtcggg
      361 caagtccacg ctgtctcacg tcctgatggg ccgagccgac tacgaagtca ccgcgggctc
      421 gatcaccctc gacggcaccg agctgctcgg gatgccgacc tggcgtcgag ccgagctcgg
      481 cttgttcctc gcgatgcagt acccgatcag cgtcccgggc gtccccgtcg gggcgctcct
      541 cggtgccgcc gcggcagccc gcggcgaggc cgtcgacgac ctcgacgccc gagtgcacgc
      601 cgaagccggt cggctcgcca tcgccgacga gttcctcacc cgaggcgtca acgacgagtt
      661 ctccggcggt gagcagaagc gcatggagac catgcagctc gcgctcctgc accccaagat
      721 cgcgattctc gacgagctcg actccgggct cgacgtcgat gccctccgcg acatcgcccg
      781 ccgcgtcgcg gccctgaccc gcgacgccgc cctcggagtc ctcgccatca cccactacgc
      841 ccgactcctc agcgagcttc gtcccgaccg ggtgcacgtg ctgatggcgg ggcggatcgt
      901 gaccagcgga ggaccggagc tcgccgacca gctcgaagcc ggcggctacg aaggtctggc
      961 gtccgagctc ggcgtcgacg agctcacgat ccgtgccacc aagcccagcg atcccttcgc
     1021 cgaacccggg ttctgaccgc tcccgggttc atcccagccc ccggcggacg aggccaaatg
     1081 gcgttgggac gtcacgccgc ggccactacc ctccctgacg tgtagcgcgc ggcgttcggt
     1141 ttcccttcga cctctgggat cgcgcccgtg cctgcctcgt ctccccgctc cgacttctct
     1201 gtcgtcgctg accgtctcgc gttcagccgc ggcggccagg tcatcctcga cggggtcaac
     1261 gtcgcgatcg gcccccgaac ccggctcggg atcgtgggac cgaacggtgt cggcaagtcg
     1321 accctgctgc gcatcctcgg tggcctccag gagcccgacc gcggacaggt gacgcgggca
     1381 ccggccaccc tgcgcgtcgc ctacctcgat caggagcgcg gacgggcgca cgagacggtc
     1441 cgcgcctggt tcgagcgacg gagcggtgtg gccgccgccg ctgccacctt cgacgccgcg
     1501 accgaagcgg tcgctgcgag gcgcccgggc gccgacgacg cctacgctgc cgccctcgac
     1561 gcctacctcg acgggggctg cgccgacttc gaggcccgag tcgcgaccgt cgttgccgac
     1621 ctcggcctcg ccccggggat cctcgacacc tcgacggaca ccttgtccgg tggcgaggcc
     1681 gcacgggtcg cgctggcgtc cctggagttg gttcgcgctg acgtgctcct gctcgacgag
     1741 ccgaccaacg acctcgactt cgacggcctc gaccgactcg agcggtacct ggcgtcgctc
     1801 tcgggtggtg tcgtcgtcgt gagccatgac cgggcgttcc tcgaacggac cgtcacgtcg
     1861 gtcggcgagc tcgacgagca cacccaccgg gcccaggtgt acgccggggg atggcacgcc
     1921 tacctggaag cgcggggcac ggcgcgccgc cacgccgagg acgactacgc cacctaccgt
     1981 tcggagcggg atcgcctcgg gcgccgggcc caacgtca
//
LOCUS       DATCCH010000405         3658 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_149139, whole genome shotgun sequence.
ACCESSION   DATCCH010000405 DATCCH010000000
VERSION     DATCCH010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3658
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149139"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /gene="murI"
                     /locus_tag="VIJ44_07795"
     CDS             complement(<1..495)
                     /gene="murI"
                     /locus_tag="VIJ44_07795"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEY5165836.1"
                     /translation="MTAPGPIGIFDSGLGGLTVARSILDLVPEEPVVYFGDSGRFPYG
                     PKPGDEVLKYALQIGDLLVDRGARLVVVACNSAAAAALDTLAARLPVPVVGVIEPGLR
                     AALRVSDSRRIGVIGTVGAIASGAYQQAARRLDPTVELTCAACPGFVEFVEAGDVESE
                     QVHIL"
     gene            complement(492..1481)
                     /locus_tag="VIJ44_07800"
     CDS             complement(492..1481)
                     /locus_tag="VIJ44_07800"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014394399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEY5165837.1"
                     /translation="MNTMDGDIEDAVRILRRGGLVAFPTETVYGLGADAANPDALRRL
                     YAVKGRPRSHPVIVHLATGSLAALAPWTEAFTPAARALADACWPGPVTLVVRRAGGVP
                     AEVTGGSDTVAIRVPDQPVAQQLLTRFGGGIAAPSANRFGRVSPTTAAHVRADLDTDV
                     DIILDGGPCRVGLESTVVDCTDHDPLILREGALAREEVEAIVGRPVPLRTDGSRAAPG
                     TLDSHYAPSTRVVLVTDHEIGSRAARLVADGQRVGVLTQHPNADLPGNVEVLDAPGNL
                     DDLAHELYALLRDADDRHLDVLLAVPPAPVGIGAAVADRLTRAAGLRVPERAP"
     gene            1615..1992
                     /locus_tag="VIJ44_07805"
     CDS             1615..1992
                     /locus_tag="VIJ44_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5165838.1"
                     /translation="MITRVRTVSVCVADQDRARDFYVDRLGFEVRTDEPMGPPGSPRW
                     LEAAPPGGDTTLVLLTPPGMEGRIGTHTGYVLACDDLAATHHVLLAAGVAFADEPSEQ
                     PWGLWATFEDPDGNAFGLVQRSE"
     gene            complement(1997..2932)
                     /locus_tag="VIJ44_07810"
     CDS             complement(1997..2932)
                     /locus_tag="VIJ44_07810"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010839545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEY5165839.1"
                     /translation="MKTETVLGLIGETPLVGIHALSPNPSVRIYAKLEGQNPGGSSKD
                     RIALAMIDLAERDGTLKPGDTILEPSSGNTGIGLALVAKLRGYHLRVVLPDNVSVERR
                     QLLEIFGADIVPSDGAEGSNGAIRRAEQLAADEPSLRLLFQYGNPANPLAHYEGTGPE
                     IWRDCPEVDVFVAGLGTSGTLMGVGRFLKEHKPDVRIVAVEPPAGELVQGLRSLDDGF
                     VPPIFDPTVLDRKLIVRPRESIEWLRRLLDECGVFAGISSGAAVAGAVKAAADLDEGT
                     IVVLLPDGGWKYLSSGAWTDPLDVVAERATRINYW"
     gene            complement(3020..3295)
                     /locus_tag="VIJ44_07815"
     CDS             complement(3020..3295)
                     /locus_tag="VIJ44_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HEY5165840.1"
                     /translation="MPVEVRLPTLLRSQAGGAATISADGATVGDVFRTLIAEHPGLAD
                     SLIDDTGQLHRFVNVYRNDDDIRYLDQLDTKLADGDVISILPAVAGG"
     gene            complement(3306..>3658)
                     /locus_tag="VIJ44_07820"
     CDS             complement(3306..>3658)
                     /locus_tag="VIJ44_07820"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HEY5165841.1"
                     /translation="CGLLAGPAETDGEPTGVVTSVYPCTNADASARTYTVDSRELLRA
                     MRDAQSRHEDLVGVWHSHTHSDAYPSDTDVRQALEPFWIYVLVSLMHAEPVLRAYRIR
                     DGAVGECEVVLADS"
BASE COUNT          534 a         1234 c         1384 g          506 t
ORIGIN      
        1 gaggatgtgc acctgctcgg attcgacgtc gccggcctcc acgaactcca cgaaaccggg
       61 acaggcggca cacgtcagct ccacggtcgg gtcgaggcgc cgcgccgcct gctggtaggc
      121 ccccgacgcg atggccccga cggtgccgat gacgccgatc cgccgtgagt cgctgactcg
      181 aagcgcggct cgcagcccgg gctcgatcac gccgacgacg gggaccggga ggcgtgccgc
      241 cagggtgtcg agtgcggccg cggcggcgct gttgcaggcc acgacgacga ggcgggcgcc
      301 ccggtcgacg aggaggtcgc cgatctgcaa cgcgtacttc agcacctcgt cgccgggctt
      361 gggcccgtag gggaaccgcc ccgagtcccc gaagtacacg acgggctcct cgggaacgag
      421 gtcgaggatg gagcgggcga cggtgaggcc gccgaggccg gagtcgaaga ttccgatggg
      481 gccgggcgcc gtcacggggc acgctccggc acccgtaggc ccgcggcgcg ggtcaggcgg
      541 tccgcgaccg cggctccgat cccgaccggc gccgggggga ctgccagcag cacgtcgaga
      601 tggcggtcat cggcgtcgcg gagcagcgcg tagagctcat gggcgaggtc gtcgaggttc
      661 ccgggagcgt cgaggacttc gacgttgccc gggaggtcgg cgttcgggtg ttgggtgagc
      721 acaccgacac gctgaccgtc ggccaccagc cgggcggcgc gcgacccgat ctcgtggtcg
      781 gtgaccagca ccacgcgggt actcggcgcg tagtgcgagt cgagggtgcc gggcgcggcg
      841 cggctcccgt cggtgcgcag cgggaccgga cgacccacga tggcttcgac ctcctcgcgg
      901 gccagcgcgc cctcgcgcag gatcagggga tcgtggtcgg tgcagtcgac gacggtcgac
      961 tcgaggccga cgcgacacgg gccgccatcg aggatgatgt ccacatcggt gtcgagatcg
     1021 gctcgcacgt gcgcggcggt ggtcgggctc acccgcccga accggttcgc cgacggggcc
     1081 gcgatgccgc cgccgaaccg ggtgagtagc tgctgggcca ccggttggtc gggaacgcgg
     1141 atggcgacgg tgtcggatcc gccggttacc tcggcgggta cgccgccggc tcgtcgcacc
     1201 accagggtga cgggcccggg ccagcacgcg tcggcgagcg ctcgcgccgc cggtgtgaag
     1261 gcctcggtcc acggggccag ggcggccagg ctgccggtgg cgaggtgcac gatcaccggg
     1321 tgggaccggg ggcggccctt gacggcatag agccgtcgga gcgcgtcggg gttggcggcg
     1381 tcggcaccga gcccgtagac cgtctcggtc gggaacgcca ccagcccgcc ccgtcggagg
     1441 atccgcacgg cgtcctcgat gtcgccgtcc atggtgttca tcgccttcat catcgccgac
     1501 cgccggtcac cagcgtcgag accgggcggc ctcgaccgga cgccggcggg tcgggtgata
     1561 aagctcaccg gcggtgccgc cgctgagacg acgggcgggc gggaggagca caggatgatc
     1621 actcgggtgc gtacggtcag cgtctgcgtc gcggatcagg atcgggcccg ggacttttac
     1681 gtcgaccggt tgggcttcga ggtgcggacc gatgagccga tgggccctcc ggggtcgccg
     1741 cgatggctcg aggcggcacc cccgggcggc gacaccacct tggtgctctt gaccccgccg
     1801 ggcatggaag gccgcatcgg cacgcacacg gggtacgtgt tggcctgcga cgatctcgcg
     1861 gccacgcatc acgtgctcct cgcggcgggg gtcgcgttcg cggacgagcc cagcgagcag
     1921 ccctgggggc tgtgggccac cttcgaagac cccgacggca acgccttcgg gctcgtgcag
     1981 cgcagcgagt agggcatcac cagtagttga tccgcgtggc gcgttcggcg acgacgtcga
     2041 gcgggtcggt ccacgcgccc gacgacaggt acttccaacc gccgtcgggg aggagcacga
     2101 cgatggtgcc ctcgtcgagg tcggcggcgg ctttcaccgc gcccgctacc gccgctcccg
     2161 acgagatccc ggcgaagaca ccgcactcgt cgagcagccg acgcagccac tcgatcgact
     2221 cgcggggtcg gacgatgagc ttgcggtcca ggaccgtcgg gtcgaagatc ggcgggacaa
     2281 agccgtcgtc gagcgaacgc agaccctgga cgagctctcc ggccggcggc tccacggcga
     2341 cgatccggac gtcgggcttg tgttccttga ggaaccgccc gacccccatg agcgtcccgc
     2401 tggtcccgag ccccgcgacg aacacgtcca cctcggggca gtcgcgccag atctcggggc
     2461 cggtcccttc gtagtgggcg aggggattcg ccgggttgcc gtactggaag agcagccgca
     2521 ggctcggctc gtcggccgcc agctgctcgg cccggcggat ggcgccgttg ctgccctcag
     2581 ccccgtccga ggggacgatg tcggcgccga agatctccag caactggcgc cgctcgaccg
     2641 agacgttgtc gggcaacacc acccggaggt gatagccccg cagcttcgcg acgagggcca
     2701 ggccgatgcc ggtgttgccc gaggagggtt cgaggatcgt gtcaccgggc ttcagggtgc
     2761 cgtcgcgctc ggcgagatcg atcatggcca gcgcgatgcg gtccttcgac gacccgccag
     2821 ggttctggcc ctcgagcttg gcgtagatgc gcaccgaggg gttcggcgaa agcgcatgga
     2881 tcccgaccag cggcgtttca ccgatcaggc cgaggacggt ctcggtcttc atcgccgctg
     2941 cgcttgcggg cgggcgcgcg acgcgagcga cgcgagcgac tcgggcgagg cgtcccggct
     3001 cgccgcgaca cctcccgggc taaccgccgg caacggcggg caggatcgag atgacgtcgc
     3061 cgtccgccag tttcgtgtcg agctggtcga gatagcggat gtcgtcgtcg ttgcggtaca
     3121 cgttgacgaa acggtgaagt tggccggtgt cgtcgatgag gctgtccgcc agcccgggat
     3181 gctcggcgat cagcgtgcgg aagacatcgc cgaccgtcgc gccgtcagcg gaaattgtgg
     3241 ccgcaccacc ggcttgtgag cgcagcagcg tcgggagtcg gacctcgacc ggcatgcgac
     3301 gcagtctagg agtccgcgag gacgacctcg cactccccca ccgcgccgtc ccggatccgg
     3361 taggcgcgca gcaccggctc ggcgtgcatg aggctcacga ggacgtagat ccagaacggc
     3421 tcgagcgcct gacggacatc ggtgtcggac gggtaggcgt cactgtgcgt gtgggaatgc
     3481 cagacgccga cgaggtcctc atgtcgcgac tgggcgtcgc gcatggcgcg gaggagctcc
     3541 cgcgagtcca cggtgtaggt gcgagctgac gcgtccgcgt tcgtgcacgg atagaccgag
     3601 gtcacgaccc cggtgggctc gccgtcagtc tcggccggcc ccgcgaggag gccgcaag
//
LOCUS       DATCCH010000406         2006 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_611340, whole genome shotgun sequence.
ACCESSION   DATCCH010000406 DATCCH010000000
VERSION     DATCCH010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611340"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..1243)
                     /locus_tag="VIJ44_07825"
     CDS             complement(44..1243)
                     /locus_tag="VIJ44_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011254935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase"
                     /protein_id="HEY5165842.1"
                     /translation="MTLKDKAAIVGIGATPYYRRGQSLPQTPLELAGKATLAALEDAG
                     LTVDDVDGLALYSMGLGGDTSLFAQTIGIPEVRFTAMLTGGGGGAAGSVGLAAAAVAS
                     GMATCVVSLMTLQQAASRFGASYAPRGKPGAQYSAPPSPEGNFIQPAGLMAPGQMFAV
                     LANRHMHRYGTSRDHFAEIVISTRENARRRETALMREPLDRDQYFAARMIAEPLCLYD
                     FCLECDGAVAVVTVSTERAKDLRQPPVAITASAHGGHGRWGQAITWMNMPDDEFASSG
                     HRPVARRLYEMAGLGPSDVDVALLYDHFSPMVLMQIEDYGFCDVGEGGRFVADGNIRW
                     PEGSLPVNTHGGNLSEAYIIGMTHVKEAVEQLRGSAVNQVAGARVALATGGPASLPTS
                     SLLLTRP"
BASE COUNT          335 a          689 c          697 g          285 t
ORIGIN      
        1 cggagggtca cggcgcttgc cgccgatgag gtcggtgcgt cgttcatggt cgggtcagca
       61 gcagtgagga cgtcggcagg gaagccgggc ctcccgtcgc cagcgccacc cgggcgccgg
      121 ccacctggtt gaccgcagag ccgcgcaact gctcgacggc ttccttcacg tgcgtcatcc
      181 cgatgatgta ggcctccgaa aggttcccgc cgtgggtgtt gaccggaagc gacccctcgg
      241 gccagcggat gttgccatcg gcgacgaacc ggccgccctc tccgacgtca cagaacccgt
      301 agtcctcgat ctgcatcagc accatcgggg agaaatggtc atagaggagc gcgacgtcca
      361 cgtcggacgg tccgaggccc gccatctcgt agaggcggcg agcgacaggt cggtggcccg
      421 acgacgcgaa ctcgtcgtct ggcatgttca tccaggtgat ggcctgaccc caccgcccgt
      481 ggccaccgtg agccgacgcg gtgatcgcca ccggcggctg ccgcaggtcc ttcgcccgtt
      541 ccgtcgacac ggtcacgacc gcgacggcgc cgtcacactc gaggcagaag tcatagagac
      601 acaggggctc ggcgatcatc cgggccgcga agtactggtc gcgatcgagc ggttcacgca
      661 tcagagcggt ctcgcggcga cgggcgttct cacgggtcga gatgacgatc tcggcgaaat
      721 ggtcgcgact cgtcccgtag cggtgcatgt gccggttggc gagcaccgcg aacatctgac
      781 ccggagccat gaggccggcc ggctggatga aattgccctc cggggacggg ggcgccgagt
      841 actgggcgcc gggcttgcct cgcggcgcat acgacgcccc gaaccggctc gcggcttgtt
      901 gcagcgtcat gaggctgacg acgcaggtcg ccatgcccga cgcgaccgcc gccgcggcga
      961 gccccaccga accggcggct ccgccaccgc caccggtgag catggcggtg aaccgcacct
     1021 cggggatccc aatggtctgc gcgaacaagg acgtgtcacc gccaagaccc atgctgtaaa
     1081 gggcgagacc gtcgacatcg tcgacggtga ggccggcgtc ctcgagcgcc gccagggtgg
     1141 ctttgccggc aagctcgagc ggtgtctgtg gcagggactg gccacgcctg taatacggcg
     1201 tcgcgccgat cccgacgatc gccgccttgt ccttcagggt catgccgggg gcatcatggc
     1261 acggtgcgtc gcgacgacat gggggcagct gggccaagtg cgacgccgcg gtcgtgtggc
     1321 aaggcacgca gatgtcgtgt tacgccgaga gcaccccggt cgtggtcgtg gacggtgacg
     1381 ggcacgagct gatcgatgtc gacgggcgcc gctacctgga cgccatcgca tcgctgtggg
     1441 tgaacacggc gacaccgtcg acctcgtgcg aggcctccag cccgatctcg tcgcttcggt
     1501 tgccgacccg agcctcggcg cgatcaactc ggtccgtctg gccgccgcac cgctcgaccc
     1561 ccgaagagca agccggctgg ccccggtggt cgtggtcctc aaccgcttcg acgccgccga
     1621 cctgctccat cgaaccaacc ggtcatggct gagcgagcgg ttcacctgcg gctggtgccc
     1681 gacgccgccg cgctcgccga gctggtgcgg gccgaggcga cgacggaccg acagccagct
     1741 gaggcgggac gggcgccgaa gcggcccccg ggagaccggc cagggggaac gaaaccggcc
     1801 tcaccggggt cgccgctcga ggctcccgca tcctcgccga cccggatagc gccctgaggt
     1861 ccagccgatg aacaggatgt aacaccggca cgaagagcgg gaccatctcg ggcggtgcga
     1921 gcgttgctac cagcacgacg acacggaggt caccaggaat gttcggaacc aagtcacgga
     1981 tgatcgagcc cgaccaggcc ctacca
//
LOCUS       DATCCH010000407         2080 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_444899, whole genome shotgun sequence.
ACCESSION   DATCCH010000407 DATCCH010000000
VERSION     DATCCH010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444899"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            319..792
                     /locus_tag="VIJ44_07830"
     CDS             319..792
                     /locus_tag="VIJ44_07830"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEY5165843.1"
                     /translation="MPDQRNEPFDRPPRDEIPVISRAHVAAQESSDDDLVWVAAGMHH
                     VIIVTTGRRSGREHKVTVPYWVDSDGHRVVVASFAGSAQHPAWYLNLADRSANPEVLC
                     KVQDGQFWSRPETLSGHDYVTTWAALTTDRPFYNDYQSETERRIPLVRLAENRPA"
     gene            complement(853..1644)
                     /locus_tag="VIJ44_07835"
     CDS             complement(853..1644)
                     /locus_tag="VIJ44_07835"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY5165844.1"
                     /translation="MTFVDTSRATPGVTDPHRSAKNSRTLKTTIWLPAHPNGTYPLIA
                     FAHGCGGSARDYAPLLQAWAAAGYVVAAPDFPVSSLHSPGVACATDIANQPGDISFVI
                     TRVLALDRARHGLGHIVDRAHIGVAGHSLGGVTTLGVAFRSCCRDRRIMAAISFAGTP
                     LIRGTDFHGVATPLMLVHGDADQTVNYSASVTAFAKAAGPRDLLTILGGMHSGYLVGS
                     DPVSQAVRRATLDFWAGYLRNDSAALARLRSDAVIPGATTLQSAG"
     gene            complement(1838..2017)
                     /locus_tag="VIJ44_07840"
     CDS             complement(1838..2017)
                     /locus_tag="VIJ44_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165845.1"
                     /translation="MIERPEVVAAHRELCEASDAVRHLLVVETLTQTQIDEARDRLAR
                     AYERAQAIDAEALHV"
BASE COUNT          347 a          691 c          728 g          314 t
ORIGIN      
        1 ggccggccac gaggaccagt cggcgagcgt tccccgatcg aaccacccca gccccgcgac
       61 cactcacgag gacggacggg ttcgctgggg cgagccgcgc tctcggccgt gcgggtgacg
      121 gggcgggacc cgaccgactc ggttcgcagc catgaggcaa tgtcggcgtc ggccgacgcc
      181 cacttgaacc ggcacccgag cgagcgacgt cccctccgtt ccccgtggtg cttgggtccc
      241 gccctgggga tcccttccga ccgagatgag tgccacggcg gacggatcgt gctcgtggac
      301 cggcactagc gtcgcccgat gcccgatcag cgcaacgagc cgttcgaccg accgccccgc
      361 gacgagatcc cggtcatcag tcgcgcccac gtggcggcac aagaatcctc ggacgacgac
      421 ctcgtgtggg tcgccgccgg gatgcaccac gtgatcatcg tgacgaccgg acgacgatca
      481 ggtcgagagc acaaggtcac cgtgccctac tgggtcgact cggacgggca tcgcgtcgtc
      541 gtcgcctcgt tcgccggttc ggcgcagcac ccggcgtggt atctcaatct cgctgaccgc
      601 tcggcaaacc cagaggtcct ctgcaaagtc caagacggcc agttctggtc gcgacccgag
      661 accctgtccg gccacgacta cgtcacgaca tgggccgcgc tcaccacgga ccgtcccttc
      721 tacaacgact accaatccga gacggaacgg cggattccgc tggtgcgcct ggcagagaac
      781 cggcccgcct gagccggggc cagacctggt tggcactcgg tcaaggtggc cggcacgccg
      841 acccggggct cgtcagccgg cgctctgcag ggtcgtcgcc ccaggtatga ccgcgtcgga
      901 gcgaagacgg gcaagagcgg cggaatcgtt tcgcaaatag ccggcccaaa agtcaagggt
      961 ggcgcgccgg acggcctgcg acacagggtc gctgccgacc agatatccgc tgtgcatccc
     1021 accgaggatg gtgagcaggt cgcgtggacc ggcggccttg gcgaacgcgg tcacgctcgc
     1081 ggagtagttc accgtctggt ccgcgtcgcc gtgtacgagc atgagcggcg tcgccacacc
     1141 gtgaaagtcg gtaccgcgga tcagcggcgt accggcgaag gagatcgctg ccatgatccg
     1201 gcgatcacgg cagcaggagc ggaacgcgac cccgagggtg gtgacaccac cgagcgagtg
     1261 gccggccacg ccgatgtgtg cacggtcgac gatgtggccg aggccgtgac gggctcgatc
     1321 gagcgcgagg acccgggtga tgacgaagct gatgtcgccg ggttggttgg cgatgtcggt
     1381 ggcgcatgcc accccggggg aatggaggct cgagaccggg aagtcgggag ctgccacgac
     1441 atacccggcc gcggcccagg cctggagcag tggggcgtag tcgcgtgcgc ttccgccgca
     1501 cccgtgcgcg aacgcgatca atggatacgt gccattgggg tgggcgggta gccaaatcgt
     1561 ggtcttgagc gtccggctgt tcttggcgct gcgatgcgga tcggtgacgc caggggtcgc
     1621 ccgactcgtg tccacgaacg tcacggtgcg aacggggggg ccgctcggct cgccctgggc
     1681 cccggcgggc actgcggcgc tcgatgaagc tacgaccgtg agggctgcca ccgccgaaag
     1741 gacccacgcc gcggggtgaa ccctccccgt cactgtcacc gccttgcgta ccagttgcgc
     1801 gcgaccccaa atggttcaaa tcgacgacgc aattggatca gacgtgtagt gcctcggcgt
     1861 cgatggcctg ggcgcgttcg taggctcgag cgaggcggtc gcgcgcctcg tcgatctggg
     1921 tctgggtgag ggtttcgacc accaagaggt gtcggacggc gtcactcgcc tcgcagagtt
     1981 cacggtgggc ggcaaccacc tcaggacgct caatcacgct cctcaatcta gtccctagct
     2041 gggtggcgat attggccgtg cccgagctgg acgattggtc
//
LOCUS       DATCCH010000408         2213 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_358493, whole genome shotgun sequence.
ACCESSION   DATCCH010000408 DATCCH010000000
VERSION     DATCCH010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358493"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1174)
                     /locus_tag="VIJ44_07845"
     CDS             complement(<1..1174)
                     /locus_tag="VIJ44_07845"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HEY5165846.1"
                     /translation="MAELCEPDAIYWCDGSQAEYEHLCRALVDAGTFVELDPERRPGS
                     YWATTDPSDVARVEDRTFICSEQEGDAGPTNNWMDPAEMRGRLQDLFRGSMHGRTMYV
                     VPFSMGPLGSPLSYIGVEITDSPYVALSMIVMTRAGKGALDVLGDDGPFVPCLHSLGA
                     PLADGEADTVWPCNPDEKWIVHFPETREIWSYGSGYGGNALLGKKCFALRIASVIARD
                     EGWLAEHMLILKLTSPKGAVRYIAAAFPSACGKTNLALLVPTIPGWKAECVGDDIAWM
                     KFGADGRLYAINPENGFFGVAPGTNRKTNPNAMRTVEANTVFTNTALTDDGDIWWENI
                     DGPVPEHLTDWHRQDWTPASDTPAAHPNSRFCVPLDQCPVLAPEYDDTDGVPISAFL"
     gene            1328..1582
                     /locus_tag="VIJ44_07850"
     CDS             1328..1582
                     /locus_tag="VIJ44_07850"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEY5165847.1"
                     /translation="MSWLSWIGVGFLAGVLAKGVTGRHRPGCLGTIIIGIVGGIVGGA
                     LFKAAGGEGINSFSLYSILVAFVGATVLLFVWGLLARNRG"
     gene            complement(1583..1810)
                     /locus_tag="VIJ44_07855"
     CDS             complement(1583..1810)
                     /locus_tag="VIJ44_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165848.1"
                     /translation="MQGVVRIYDPQTGEGIVVRDTDRAELILAPEALAGSLFRMLRQG
                     QRINFELDGDSRVIRVRIGAEADLGLPTAQI"
     gene            complement(1810..1998)
                     /gene="rpmB"
                     /locus_tag="VIJ44_07860"
     CDS             complement(1810..1998)
                     /gene="rpmB"
                     /locus_tag="VIJ44_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018655297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEY5165849.1"
                     /translation="MASVCDVCGKAPRFGMRLSHSHRRTKRRWNPNVQRVRAIVNGTP
                     KRVHVCTSCLKAGKVQKP"
BASE COUNT          340 a          731 c          794 g          348 t
ORIGIN      
        1 acaggaacgc ggagatcggg accccgtcgg tgtcgtcgta ctcgggggcg aggacggggc
       61 actggtcgag ggggacacag aaccgggagt tcgggtgcgc cgcgggggtg tccgacgccg
      121 gtgtccagtc ctggcggtgc cagtcagtga ggtgctcggg gaccgggccg tcgatgtttt
      181 cccaccagat gtcaccgtcg tcggtgaggg cagtgttggt gaagaccgtg ttggcctcga
      241 cggtgcgcat ggcgttgggg ttggtctttc ggttcgtgcc cggcgcgaca ccgaagaacc
      301 cgttctcggg gttgatcgcg tacagacgcc cgtcggcacc gaacttcatc cacgcgatgt
      361 cgtcaccgac gcactcggcc ttccaacccg ggatggtcgg aaccaacagc gccaggttcg
      421 tcttgccgca cgccgagggg aacgcggcgg cgatgtagcg caccgccccc ttcggggagg
      481 tcagcttgag gatcagcatg tgctcggcga gccagccctc gtcgcgggcg atcaccgacg
      541 cgatccgcaa cgcgaagcat ttcttcccga ggagggcgtt gcccccgtac cccgagccgt
      601 acgaccagat ctcccgggtc tcggggaagt gcacgatcca tttctcgtcg gggttgcacg
      661 gccagacggt gtcggcctcg ccgtcggcga ggggggctcc gagcgaatgc aggcacggga
      721 cgaacgggcc gtcatcgccg aggacgtcga gggcgccctt cccggcccgg gtcatgacga
      781 tcatcgacag cgccacgtac ggcgaatcgg tgatctcgac cccgatgtag gagaggggcg
      841 agcccagcgg acccatgctg aacgggacca cgtacatggt ccgaccgtgc atggagccgc
      901 ggaacaggtc ctggaggcgg ccccgcatct cggccggatc catccagttg ttggtcggac
      961 cggcgtcgcc ctcctgctcg gagcagatga aggtgcggtc ctcgacccgg gccacgtcac
     1021 tcgggtccgt cgtcgcccag taggagccgg ggcgccgctc cggatcgagc tcgacgaagg
     1081 tgccggcgtc gacgagagcc cggcacagat gctcgtactc ggcctgggag ccgtcgcacc
     1141 aatagatggc gtcgggttcg cagagctcgg ccatggtctc gacccagccg agcaggcgct
     1201 catgcttggt cggggcgtcc accacgagtg acaacgaagc agctcctcgg cgggcaacgg
     1261 tggacggcga gtctactggg ggtccctccc gagccgccga gtcgagccgg tcgatacgct
     1321 gccggccatg agctggctgt cgtggatcgg ggtcggattc ctcgccggcg tgctggccaa
     1381 gggcgtcacc ggccggcacc ggccgggatg cctggggacg atcatcatcg ggatcgtggg
     1441 cgggatcgtc gggggcgcgc tgttcaaagc ggccggcggg gagggcatca acagcttctc
     1501 gctgtactcg atcctcgtcg cgttcgtcgg ggcgacggtc ctgctgttcg tttggggcct
     1561 gctggcccgc aaccggggct gatcagatct gggcggtggg caacccgagg tcggcctccg
     1621 cgccgatccg gacccggatg acgcggctat ccccgtcgag ctcgaagttg atgcgctgac
     1681 cctggcggag catgcggaac aacgagccgg ccagcgcctc gggggcgagg atcagctcgg
     1741 cccggtcggt gtcgcgcacc acgatcccct ccccggtctg ggggtcgtag atccgcacga
     1801 cgccctgcat cagggctttt ggaccttccc ggccttgagg cacgaggtgc aaacgtggac
     1861 acgcttcggg gtcccgttca cgatggcccg gacccgctgc acgttggggt tccagcgccg
     1921 cttggtgcgc cgatgggagt ggctcaggcg catgccgaac cgcggcgcct tgccgcagac
     1981 gtcacagacc gatgccatcg cgcactccga ggtcgagggg acccgcgacg atacccggcc
     2041 acgccgaccg cgcgcgaagc ggacccaact acgatccggc gcgtgccggt cctcgacaac
     2101 atcgacgggg ccgccctcgc acgggttgtt gccacctacc gtgacgccct gcgcgcccat
     2161 caagaggagc tgaaccggct caacgtctac ccggtgcccg acggtgacac cgg
//
LOCUS       DATCCH010000409         4321 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_237686, whole genome shotgun sequence.
ACCESSION   DATCCH010000409 DATCCH010000000
VERSION     DATCCH010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4321
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237686"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="VIJ44_07865"
     CDS             <1..696
                     /locus_tag="VIJ44_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018179216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5165850.1"
                     /translation="ARGLAADGADVAVNYRRDDAAAQETVAAIEALGRRAHAYQASVD
                     DAEADTRMVEAVLSDFGYVDILVNNAGIASRGQRTADTDPAELERVWRTHAFGAFILS
                     QLVLPGMRTQPRGDIVMISSVATSFMAAHSVPYNLAKASLEALGRTLAKEEREHGIHV
                     NTVAPGLVVSEMGRRLVRATQGIEDIHDLDRRSPFGHVCTPEEVADVVRFLVSDGARY
                     LTEQRLTVDGGSF"
     gene            858..2021
                     /locus_tag="VIJ44_07870"
     CDS             858..2021
                     /locus_tag="VIJ44_07870"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEY5165851.1"
                     /translation="MQLVAIVVALVGPVLVAVVAWRVLRRQDADSRQLAAQHQQVFAE
                     QRDAAVQAAIDHVLAHNRQALTAERELAGKDLDGKKSLIDQQLATMTAKLDDVSTMVQ
                     QVEAERARSFGELNAQLSRQHEGLAALLHTTQSLREALSSTKARGQWGERMAEDVLRL
                     AGFVENVNYRKQRALDTGRGIPDYTFFLPNRLLLHMDVKFPLDNYVRFCDATSDLERK
                     RYRDDFLRDVRARVRELSDRDYVDPGGGTVDFVLLFIPNEQLYAFIHEQDETILDDAV
                     RHGVVFCSPLTLFVVLALIRQMVENLRLARTSDEILSLLGQFSDQWTKFKGQMEKVGQ
                     RIDAASKEYTALAGTRTNTLERPLRRINELRTRDLELVEDPGSDEQPPLALEA"
     gene            2093..2479
                     /locus_tag="VIJ44_07875"
     CDS             2093..2479
                     /locus_tag="VIJ44_07875"
                     /inference="COORDINATES: protein motif:HMM:NF008528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HEY5165852.1"
                     /translation="MQRLFALAVLTVIVEVTVIIEVGRRIGALNTVGLLIVVSAVGAV
                     IVKAQGLTALRRAITDLEDRRVPGAALADAALLAAAGALVLIPGFVTDVFGLLLLVPP
                     VRSGVRLVLRRRWSKRFVVQGYELEA"
     gene            complement(2689..3441)
                     /locus_tag="VIJ44_07880"
     CDS             complement(2689..3441)
                     /locus_tag="VIJ44_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165853.1"
                     /translation="MLPFERLRALARYAGDDTGLVVEAADCLADFAVDPTQLVTVCRR
                     LLAHHPSSGPLWWLCTQVLTATDPSGGARDAVRALERDRTATRLSSLLPFPHDEPIAV
                     LGWPDVTGEALAERPDLDIVVVRTDYRPPRAADRRVRVVDEVEAMAASPSHLLVEVLA
                     AGPTTAVVPHGVGDLHRSLDDVALWLVVPVGRLLPARLFDVLRQQVGEEPDVEAIDVT
                     VATQTAGPWGLDVPARLGAHVNCPVAPELLRI"
     gene            complement(3447..>4321)
                     /locus_tag="VIJ44_07885"
     CDS             complement(3447..>4321)
                     /locus_tag="VIJ44_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY5165854.1"
                     /translation="AHGIPEVKSHYLASLHRADIIACQLFSEPVAGSDLAGIQTRAER
                     DGDEWILNGQKVWTSGAHYSDIGEIITRTSTDKPKHKGLTMFLVDMKAPGVEVRPLRQ
                     MTGGASFNEVFFADVRVLDTHRLGDVDEGWTVALTTLMNERASIGGGGAAGFSLSVTR
                     LRELAYHYDLADDPIMRQRLADIYVRMTVARYTNLRAMAKIRAGQLPGPEMSIAKLAL
                     TQNMQRVAQTLGELLGPRLVADTGEWGTFAWSEFVLGLPGMRVAGGTDEVMRNIVGER
                     VLGLPKEPQGQAGR"
BASE COUNT          705 a         1516 c         1432 g          668 t
ORIGIN      
        1 gcgcgtgggc tcgccgccga cggcgccgac gtcgcggtga actatcgccg agatgatgcg
       61 gcagcccagg agacggtcgc tgccatcgag gcgctcggtc gccgcgccca cgcctaccag
      121 gcgtcggtcg atgacgccga ggccgacacc cgcatggtcg aagccgtgct ctcggacttc
      181 gggtacgtcg acatccttgt caacaacgcc gggatcgcca gccggggcca gcggaccgcc
      241 gacaccgatc ccgccgaact cgaacgggtt tggcgcaccc acgctttcgg tgccttcatc
      301 ctgagccagc tggtgctgcc ggggatgcgt acccagccgc gcggtgacat cgtcatgatc
      361 tccagcgtgg ccaccagctt catggccgcc cactcggtgc cgtacaacct cgcgaaggcg
      421 tcgctcgagg cgctgggccg cacgctggcc aaggaagagc gggagcacgg catccacgtc
      481 aacacggtcg ctcccggact cgtcgtctcc gagatgggac ggcgcctggt gcgggcaacc
      541 cagggcatcg aggacatcca cgacctcgac cgccgatccc cgttcggcca cgtctgcacc
      601 cccgaggaag tcgccgatgt cgtgcgcttc ctcgtctccg acggggcccg ctacctcacc
      661 gaacagcgtc tcaccgtgga cggcggcagc ttctgacccc accctggtgg gcgcgaagac
      721 ggggatcccg ccgatcgtcg gcaccagcct gagggccgga cccacccggc cggggcgcca
      781 ggcccggtcc gacggggcgt ttgtgcaggt cagcggccat ccctgggggt gtcacacccc
      841 cgtgtgacgc tctagccatg cagttggtcg ccattgtggt ggcgctcgtg ggacccgtgc
      901 tcgtggcggt ggtcgcgtgg cgggtgttgc gccgccagga cgccgattca cgacagctcg
      961 ctgcgcagca ccagcaggtc ttcgccgagc agcgagacgc cgcggtgcaa gccgcgatcg
     1021 atcatgtcct cgcccacaac cgccaggcgc tgaccgccga acgggagctg gccggcaaag
     1081 acctcgacgg caaaaagtca ctgatcgatc agcagctcgc caccatgacc gcgaaacttg
     1141 acgatgtctc cacgatggtc cagcaggtgg aggcggaacg ggcccgctcg tttggtgagc
     1201 tgaacgccca gctgtcccgc cagcacgagg gcctcgccgc gctcctccac accactcaga
     1261 gcctccggga ggccctctcg agcaccaagg ctcgaggtca gtggggcgag cggatggccg
     1321 aagacgtgct ccgcctcgcc ggattcgtcg agaacgtcaa ctaccgcaaa cagcgggcgc
     1381 tcgataccgg ccggggaatc cccgactaca cgttcttcct gcccaacaga ctcctgctgc
     1441 acatggacgt caagttcccg cttgacaact acgtgcggtt ctgtgacgcc acttccgacc
     1501 tcgagcgcaa gcggtaccgc gacgacttcc tccgtgacgt tcgggcccgg gtacgcgagc
     1561 tcagcgaccg ggactacgtc gaccccggcg gcggaacggt cgatttcgtg ctgctcttca
     1621 tcccgaacga gcagctctac gcgttcatcc acgagcagga cgagaccatc ctcgacgacg
     1681 cggtccgaca cggtgtcgtg ttctgctctc cgctcacact cttcgtcgtg ctcgcactca
     1741 tccgccagat ggtcgagaac ctgcggctcg cccgaacctc ggacgagatc ctgagcctgc
     1801 tgggtcagtt ctccgatcaa tggacgaagt tcaagggcca gatggaaaag gtcggacagc
     1861 ggatcgacgc cgcgagcaag gagtacaccg cgctggccgg gacccgcacg aacacactcg
     1921 agcggccgct ccgccgcatc aacgagctgc gaacccgtga cctcgagttg gtcgaagatc
     1981 cgggctcgga cgagcagcca cccctcgccc tggaggcttg agggtcgacc aaccggtgcg
     2041 tcgcccggcg cacgacgacc gccgtctcgg acggcaccgg tacgctgaga tggtgcagcg
     2101 actcttcgca ctcgcggtgc tcaccgtcat cgtcgaggtc acggtgatca tcgaggttgg
     2161 gcggcgaatc ggggcgctca atactgtcgg gctgctcatc gtggtgtccg cggtcggtgc
     2221 ggtgatcgtg aaggctcagg gcctcaccgc gttgcgtcgg gcgatcaccg acctcgaaga
     2281 ccgccgggtt cccggcgcgg cgctggccga tgctgcgctg ctcgccgccg ccggcgccct
     2341 ggtgctcatc cccggcttcg tcaccgacgt cttcgggctg ctgctgctcg ttccgccggt
     2401 ccggtcaggg gtgcgcctgg tcttgcgccg acggtggtcg aagcgcttcg tcgtgcaggg
     2461 gtatgagctc gaggcctgag tcagtacggg ccatcacagc acgagtccct ccagccgcaa
     2521 accggacagc ggtaatgcgc gtgttcctcg cgcatcgcgg cgccacagtg ccagcacatc
     2581 gtcgagaggt cctgcggtgc gggccctcga ccttcgggag cggcggggtc ggtgccggtc
     2641 ccggtcacga cctcaccgta gcgagcaggg tcgcacccaa cggtgatctc agattcggag
     2701 gagctccgga gcaaccggac agttgacgtg ggcgccgagc cgggccggca cgtcgagacc
     2761 ccacggtccc gcggtctgcg tcgccaccgt cacgtcgatg gcctccacgt cgggttcctc
     2821 gcccacctgc tgacggagga catcgaacag ccgagccggc aagagccgac cgaccggcac
     2881 cacgagccac aacgcgacgt cgtcgaggga acggtgcaga tcgccaacac cgtgcggcac
     2941 gaccgcggtc gtcgggcccg cggcgaggac ctcgacgagg aggtgactcg gcgacgccgc
     3001 catcgcctcg acttcgtcga cgacccgcac ccgacggtcc gcggctcgag gcggccggta
     3061 gtcggtgcgc accacgacga tgtccaggtc gggtcgctcg gcgagggcct cacccgtgac
     3121 atccggccag ccgaggacgg cgatcggctc gtcgtgaggg aacgggagca acgacgagag
     3181 ccgggtcgcc gtgcggtcac gctcgagggc ccgcaccgcg tcacgcgcgc cgccgctcgg
     3241 gtcagtcgcg gtgaggacct gggtgcacaa ccaccacagt ggaccgctgc tgggatgatg
     3301 ggcgaggagt cgtcgacaga ccgtgaccag ctgggtggga tcgacggcga agtccgcgag
     3361 acagtccgcg gcctccacga cgaggccggt gtcgtccccc gcatagcggg cgagggcccg
     3421 gagtcgctcg aacgggagca ctagccctag cgcccggctt ggccttgggg ctccttcggg
     3481 agaccgagca cccgctcgcc gacgatgttg cgcatcacct cgtcggtgcc tcccgcgact
     3541 cgcatgcccg ggagtccgag cacgaactct gaccaggcga aggtgcccca ctcgccggtg
     3601 tcggcgacca gacgcggtcc gaggagctcc ccgagggtct gcgcgactcg ctgcatgttc
     3661 tgggtgagcg caagcttcgc gatcgacatc tccggtcccg ggagctgacc agccctgatc
     3721 ttggccatcg cccgcaggtt cgtgtaccgc gccaccgtca tccggacgta gatgtccgcc
     3781 agccgttggc gcatgatcgg gtcatcggcc aagtcgtagt ggtatgccag ctcgcgtagc
     3841 cgagtcacgc tgagactgaa cccggccgcc cctccgcccc cgatcgatgc ccgctcgttc
     3901 atcaacgtgg tgagcgcgac tgtccaaccc tcgtcgacgt caccgaggcg atgcgtgtcg
     3961 aggacccgga catcggcgaa gaacacctcg ttgaacgagg cgcccccggt catctgccgc
     4021 aagggacgaa cctcgacacc gggcgccttc atgtcgacca ggaacatcgt cagacccttg
     4081 tgtttcggct tgtccgtcga cgtgcgcgtg atgatctcgc cgatgtcgct gtagtgcgcc
     4141 cccgaggtcc acaccttctg gccattgagg atccactcgt caccatcgcg ctcagcgcgc
     4201 gtctggatac ccgcgagatc ggacccggcg acgggttcgg agaacagctg gcaggcgatg
     4261 atgtcggccc ggtgcagcga cgccagatag tggctcttca cctcggggat gccatgggcg
     4321 a
//
LOCUS       DATCCH010000410         4487 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_25449, whole genome shotgun sequence.
ACCESSION   DATCCH010000410 DATCCH010000000
VERSION     DATCCH010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4487
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25449"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="VIJ44_07890"
     CDS             <1..487
                     /locus_tag="VIJ44_07890"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY5165855.1"
                     /translation="PDEPLATADELAPLPVLGMELGVHGMDHTPTIGMDDTALDTHVV
                     RSRTDLQALIGAPARSFAYPFGKHDAAARNAVAAAGYDVSFAVAREAGRFAADRVFVR
                     RGDPLPLFRFKLSEPYRLLSRGAGRMSGLRRRVRTVVHLVSRPQGRVRSRSTNPGEID
                     E"
     gene            480..1151
                     /locus_tag="VIJ44_07895"
     CDS             480..1151
                     /locus_tag="VIJ44_07895"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016433766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEY5165856.1"
                     /translation="MSEQVFNDGAAFGSRCVDVVDIARRTLDLALSLVLLLFLALPMV
                     LIAAAVQMDSGGPALFRQRRVGRDGRVFTIFKFRTMHPNCDDGRLRDLVARELNGEDT
                     SEGGSFKLSNDPRVTRIGRLLRRTSLDELPQILNVVRGDMSLVGPRPCLEWEAEMFAP
                     EFRSRFTVRPGITGLWQVSGRSELGTVDMLKLDLEYVRHRTLWIDIVIMLRTAPTLLR
                     GSGAR"
     gene            1148..2122
                     /locus_tag="VIJ44_07900"
     CDS             1148..2122
                     /locus_tag="VIJ44_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016433767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY5165857.1"
                     /translation="MTTLFLATTGGHLTQLAGIAERIPHDPDSVWVTHSNEQSESLLA
                     DRDVQYIPYVGVRDVRGVLGCVPVAHALHGRRHITRAISTGSGIALGFLPYLAARGVE
                     CHYIESAARVAGPSVTGRLLRRVPRVHTYTQHRHWSDEHWPYGGNGFDAYEPVCESRT
                     LGDPIRVVVTVGTAREFPFRRLMVRLSKLLAPGGGLEQATGLPVEVLWQTGGAPVDDL
                     PITPIPFLPVADLNAALVRADIVVSHAGTGSALADLAAGRYAVIVHRIAQFGEAGDDH
                     QQELARELDARGLALHRDASEITVDDLLATLSSGVRRVAEVPAFELRP"
     gene            2119..3171
                     /locus_tag="VIJ44_07905"
     CDS             2119..3171
                     /locus_tag="VIJ44_07905"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5165858.1"
                     /translation="MTTSTIVAADPLTDEAWLGLACTPRASLFTSPPWINAVCGTYGF
                     TPRSVVAVDHRGEPTGGFAWVTIDDARGTRACSLPFSDRADPLVDDETGWRSLFDAAD
                     LGTAHPYALRCLDDSPAVADPRLRITSEAAWHGTPLDATPDELYRRISSASRRAIAVS
                     ERAGVRVEVRTDVDAVRSFHRLHVRLRKKKYRLLAQPMEFFERIWREFAPSDRINTLL
                     ACFRDEVIAGAVFLEWNGAFYYKFGASAAEHLAFRPNDAIYWTGIRCAIERGLGLVDW
                     GLSDLDQPGLVAFKRKWASTERRLLTLRAGMRAPRPEQVRFASELSELTRLLTDETVP
                     DAITTQAGALLYRYFC"
     gene            3203..4471
                     /locus_tag="VIJ44_07910"
     CDS             3203..4471
                     /locus_tag="VIJ44_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003967473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEY5165859.1"
                     /translation="MTNTQGGPVVVVGQGYVGLPLAMRATEVGHRVIGFDLDKSRVDR
                     LRRAESFIDDVTDADLEAAIASGYSATDDPAELAGFGTAVVCVPTPLRDGAPDLGFIE
                     ESARLLAPHVTCGCCVILESTTYPGTTEEVFEPILEAGSGLRTGRDFHLGYSPERIDP
                     NNASFGLRNTPKIVSGVDEASAVAVRAFYDGIVEQTVAAPAVREAEMAKLLENTFRHV
                     NIALVNELAIYCHELGIDVWSVIDLAGTKPFGFMKFTPGPGVGGHCLPIDPSYLSWRV
                     RRALGRTFRFVEIANDVNDNMPGYVVTRVVEHLNRRRLAVNGSRVLLIGLTYKRNSGD
                     ARQSPAVAVARRLKHLGAELRAVDPLLTAADVPDGVELVASVADEVAAADVIVVLTDH
                     DDIDWDLIGRHAGRVLDTRNRLAGSVVDRL"
BASE COUNT          647 a         1597 c         1527 g          716 t
ORIGIN      
        1 ccccgacgag ccactcgcca ccgccgacga gctcgccccg ctgccggtcc tcggaatgga
       61 gctcggagtg cacgggatgg accacacccc gacgatcggc atggacgaca ccgccctgga
      121 cacccacgtc gtccgttctc gtaccgacct ccaggcgctg atcggggcgc ccgcgcgttc
      181 gttcgcctat cctttcggga agcacgacgc cgccgcccgc aatgccgtgg ccgccgccgg
      241 gtacgacgtg tccttcgccg tcgcccgcga ggccggccgt tttgcggcgg accgcgtctt
      301 cgtccgccgc ggcgacccgt tgccactgtt ccgtttcaag ctgtccgagc cgtaccggct
      361 cctttcacgg ggggccggac gaatgtccgg cctgcggcgg agggtgcgga cggttgtcca
      421 cctcgtgtcc cgtccacaag gtcgcgtacg gagtcgatcc acgaaccccg gggaaatcga
      481 tgagtgagca ggtgttcaac gacggagccg ccttcggatc ccggtgcgtc gacgtcgtcg
      541 acatagcacg tcggacgctc gatctcgcac tctcgctcgt gctgttgctg ttcctggcgc
      601 tcccgatggt cctgatcgca gcggcggtcc agatggacag cggcggaccg gccctcttcc
      661 ggcaacgccg ggtgggccgc gacggccggg tgttcacgat cttcaagttc cgcaccatgc
      721 acccgaactg cgacgacggt cggctgcgcg accttgtcgc ccgcgagctg aacggtgagg
      781 acacctccga gggcggcagc ttcaagctga gcaacgaccc ccgcgtcacc cgtatcgggc
      841 gcttgttgcg gcggaccagc ctcgacgagc tgccccagat cctcaacgtg gtgcgcgggg
      901 acatgtcgct ggtcggcccg cgcccgtgtc tggagtggga ggcggagatg ttcgcgcccg
      961 agttccgctc ccggttcaca gtccgtccgg ggatcaccgg gctatggcag gtcagcgggc
     1021 gcagcgagct gggcacggtc gacatgctga agctggatct ggagtacgtc cgccaccgga
     1081 cactgtggat agacatcgtc atcatgctcc gcaccgcgcc gacattgttg cgcgggagcg
     1141 gggctcgatg acgacactct tcctcgccac gaccggcggc cacctcacgc agctggcggg
     1201 catcgccgag cggataccgc atgatcccga ctcggtgtgg gtgacgcact cgaacgagca
     1261 gagcgagtcg ctgctcgccg accgcgacgt gcagtacatc ccctacgtcg gcgtccgtga
     1321 cgtgcgcggg gtcctggggt gcgtgccggt cgcgcacgcg ttgcacggcc gcaggcacat
     1381 cacgcgcgcg atctccaccg ggtcgggcat cgccctcggt ttcctgccct acctcgcggc
     1441 ccggggcgtg gagtgccact acatcgagag tgcggcgcgt gtcgccggcc cctcggtgac
     1501 cggccggttg ctgcgccggg ttccccgcgt ccacacctac acccagcacc ggcactggtc
     1561 cgacgagcac tggccctacg gcggaaacgg cttcgacgcc tacgaacctg tttgcgagtc
     1621 ccggacgctc ggtgacccga tccgcgtcgt agtcaccgtc ggcacggcgc gggagtttcc
     1681 gttccggcgg ctgatggtgc ggctgtcgaa gctgcttgcc ccaggcggcg ggctcgaaca
     1741 ggccaccggg ctccccgtcg aggtgctctg gcagacgggc ggcgctccgg tggatgacct
     1801 gccgatcacg ccgatcccgt tcctgcccgt cgccgacctg aacgctgcgc tggtccgcgc
     1861 cgacatcgtc gtgagtcacg ccggtactgg ttcggcgctg gccgacctcg ctgccggccg
     1921 ttacgccgtg atcgtccacc ggatcgccca attcggcgag gccggtgacg accaccagca
     1981 ggagctcgcc cgcgagctcg acgcccgcgg gctcgccctg catcgcgacg cgagcgagat
     2041 cactgtggac gacctgctcg cgaccctgtc cagcggagtg cgccgcgttg ccgaggtgcc
     2101 tgcgttcgag ctgcgtccgt gaccacttcg accatcgttg cggccgaccc cctcacggac
     2161 gaggcctggc tcgggctcgc ctgtaccccg cgtgcgagcc tgttcacctc cccgccgtgg
     2221 atcaatgccg tgtgcggcac ctacgggttc acaccgcggt ccgtcgtcgc ggtggaccac
     2281 cgcggggagc cgaccggcgg gttcgcctgg gtcacgatcg acgacgcccg gggaacgcgg
     2341 gcgtgcagcc tgccgttctc cgaccgggcc gacccgctgg tcgacgacga gacgggttgg
     2401 cgctcgctgt tcgacgccgc cgacctgggc accgcgcacc cgtacgcgct gcgctgcctg
     2461 gacgactcgc ccgccgtcgc cgacccgcgg ctgcggatca ccagtgaggc tgcgtggcac
     2521 gggacgccgc tggacgcgac gcccgacgag ctgtaccgcc ggatcagcag cgcgtcccgg
     2581 cgcgcgattg ccgtgtcgga acgcgccggc gtccgggtcg aggtccggac cgatgtcgac
     2641 gcggtgcgca gcttccaccg gctccacgtg cggttgcgta agaagaagta ccgcctgctc
     2701 gcccagccca tggagttctt cgagcggatc tggcgggagt tcgccccgag cgaccgcatc
     2761 aacacgctgc tggcgtgctt cagggacgag gtgatcgccg gggcggtctt cctcgagtgg
     2821 aacggcgcct tctactacaa gttcggggcc tcggccgccg agcatctcgc gttccggccg
     2881 aacgacgcca tctactggac cgggatccgt tgcgccatcg agcgcgggct cggcctcgtc
     2941 gactggggac tgagcgacct cgaccagccg gggctcgtgg ccttcaaacg gaagtgggcc
     3001 tcgaccgagc gcaggctcct cacactgcgc gcgggcatgc gggcaccgcg gccggaacag
     3061 gtgcggttcg cgagcgagct ttcggagctc acccggctgc tgaccgatga gacggtcccc
     3121 gacgccatca ccacgcaggc cggcgccctg ctctaccgct acttctgctg aacgagtacc
     3181 gcaaacatga ggggaaacgt tcatgacgaa cacccaaggg gggccggtcg tcgtcgtcgg
     3241 ccagggctac gtgggtctcc cgctcgcgat gcgcgccacc gaggtcggtc accgcgtgat
     3301 cggtttcgat ctggacaaga gccgcgtgga ccggctccgc cgggccgaaa gcttcatcga
     3361 cgacgtcacc gacgccgatc tggaggccgc gatcgcctcc ggatacagcg cgaccgacga
     3421 tccggccgag ctggctgggt tcggtaccgc tgtggtctgc gtgccaacac cgctgcgtga
     3481 cggggcaccc gatctgggct tcatcgagga gtcggcccgg ttgctcgccc cgcacgtcac
     3541 gtgcggctgc tgcgtcatcc tcgagtccac cacgtatccc ggaacgaccg aggaggtctt
     3601 cgagccgatc ctggaggcgg gatcggggct gcggaccgga cgcgacttcc acctcggtta
     3661 cagcccggag cgcatcgacc ccaacaatgc ctcgttcggc ctgaggaata cacccaagat
     3721 cgtctccggc gtcgacgagg cctcggccgt cgccgtccgg gcgttctacg acgggatcgt
     3781 cgagcagacc gttgcggcgc cggctgtgcg cgaggccgag atggccaagc tgctcgagaa
     3841 caccttccga cacgtcaaca tcgcgctggt caacgagctc gcgatctact gccacgaact
     3901 cgggatcgac gtgtggtcgg tgatcgacct ggccggcacc aagccattcg ggttcatgaa
     3961 gttcaccccc ggtcccgggg tcggggggca ctgcctgccc atcgacccgt cctacctgtc
     4021 ctggcgggtg cggcgagcgt tgggccggac gttccggttc gtcgagatcg ccaacgacgt
     4081 gaacgacaac atgccaggct acgtggtgac ccgggtggtc gagcatctga accggcggcg
     4141 gctggcggtc aacggcagcc gagtgctgct gatcgggctg acctacaagc gcaactccgg
     4201 tgacgcccgg cagtcgcccg cggtcgcggt ggcgcggcgg ctgaagcacc tgggtgcgga
     4261 actgcgcgcc gtcgatccgc tgctcaccgc cgccgacgtg cccgacgggg tcgagctcgt
     4321 ggcatccgtc gccgacgagg tggccgccgc cgacgtgatc gtcgtgctca ccgaccacga
     4381 cgacatcgac tgggacctga tcggccggca cgccggccgt gtgctcgaca cccgaaaccg
     4441 gttggccgga tcggtcgtcg accggctctg aaggccgggc cgtcgat
//
LOCUS       DATCCH010000411         5067 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_67400, whole genome shotgun sequence.
ACCESSION   DATCCH010000411 DATCCH010000000
VERSION     DATCCH010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5067
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67400"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2228
                     /locus_tag="VIJ44_07915"
     CDS             <1..2228
                     /locus_tag="VIJ44_07915"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HEY5165860.1"
                     /translation="RYPERVDVARRRLSRPLTFAEKILVAHADDPETVGLERGVDYSN
                     YRPDRVAMQDATAQMALLQFMTANLAQVAVPTTVHCDHLIQAHIGAAPDLKTALDTNS
                     EVYEFLRSVSSKYGIGFWKPGSGIIHQVVLEQYAFPGGMMIGTDSHTPNAGGLGMVAI
                     GVGGADAVDVMAGWPFNTRVPRLIGVRLLGRLSGWTAAKDIILRVAGILTVKGGTGAI
                     VEYFGPGTESISATGKATICNMGAEIGATCSLFPYDVRSAHYLTATGRDEISGLADEY
                     AEFLRGDPDVEADAERFYDRVIEIDLDDLEPHIVGPHTPDLDRSLDELVRVAPEERYP
                     LDISYALVGSCTNSSYEDIGRAAHVARQASAAGLKVRSPLLITPGSEQVRATIERDGL
                     LDDLEAIGATVLANACGPCIGQWQRDDIKPGERNTIVSSFNRNFPRRNDGNAETLSFI
                     GSPETVVAMALTGRLDHDFIRTPIEAPDGHQVTLEAPAADELPAKGFDPGQSGFVPPA
                     AVGDEVNVVVKPDSERLELLEPFPPWDGQDLLSLRVLMKAVGKCTTDHISPAGPWLRY
                     RGHLDNISKNLFTGVNNAFSLDEPGTGIDGRDGSTQPLPDLARSYRDAGIVWVAVGDE
                     NYGEGSSREHAAMEPRHLNGRAIIVRSFARIHETNLKKQGVLALTFADPDDYERILVD
                     DTIDIVGLGDLAPDRPVTVRIHHADGRSTDITCRHTMSDEHIGWFRAGSALNVLRGDR
                     G"
     gene            2237..2638
                     /locus_tag="VIJ44_07920"
     CDS             2237..2638
                     /locus_tag="VIJ44_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165861.1"
                     /translation="MRVAGLLSVAVILAACGGGSSGGAAHRAASTTTATSSSGGSSVN
                     IGIICSTPTDAADAVVNAWMSGDRAAARRCGTPTAVATLFGRAAGPERWVLQHCAGTV
                     CSFTHPDGTVQLTVGGSDAAGWVVARVRFGR"
     gene            complement(2647..4002)
                     /locus_tag="VIJ44_07925"
     CDS             complement(2647..4002)
                     /locus_tag="VIJ44_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_052607803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CUAEP/CCAEP-tail radical SAM protein"
                     /protein_id="HEY5165862.1"
                     /translation="MRVLLVSCYELGHQPLGVAGPAARLRAAGHEVRALDLSVDAWDP
                     SQLDWAQRVACSVPMHTATRLARQVIDEVRRRRPEVLVACYGLYGSAMVETADRALAG
                     ETDEALLAWVDGDEGDQVVHLGRDAARGGAPRPARELLPALDRYARLVRGDTEQLVAS
                     VEASRGCVHRCRHCPVPVVYDGRIRIVALDAVLADVEQRVAAGAEHVTFADPDFLNGF
                     AHARRVVHAVHDRFPDLSFDCTVKVEHVLAHPEVWPEFAAAGCVFVVSAFESVDDATL
                     GRLDKGHTVADEATAVALLREHGIEVRPSWLPFTPWTTLEQMRALLEFVARHDLVSNV
                     DPVQYTIRLLLPPGSLLLDHQDVVAHLDGYDPERASHSWRSADPAMDALQGELSAVVE
                     ARLAAGDSTPAVYQAVRAAVGLDPVAVDEERAARVPRLSEPWFCCAEPTAAQLAPLSA
                     R"
     gene            complement(4046..>5067)
                     /locus_tag="VIJ44_07930"
     CDS             complement(4046..>5067)
                     /locus_tag="VIJ44_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEY5165863.1"
                     /translation="EAARVIERLDARHPVDAVVAVDDQGTLAAARAGEQLGFPHNPPA
                     AVARTHDKAAMRDALAAAEVPQPRYAVFEDPDAVPDVGWPCVVKPTGLSGSRGVIRAD
                     DAGAARAAATRAARIAGGGSLLVEEYVPGVEVAVEALLRAGELTVLAVFDKPDPLEGP
                     YFEETIYVTPSRLPADVLARVERVTGDAARALGLTEGPVHAEVRVDEDRVWVVEVAAR
                     SIGGLCARTLRLGAGISLEEVIVRHALGLPIDDLRRAPDASGVMMLPIPRAGTLVGVQ
                     GQDEATSVPGIVGLEITIVSGRSVEPVPEGDRYLGFLFARASTPAAVEQALRAAQDRL
                     HIEIR"
BASE COUNT          782 a         1796 c         1805 g          684 t
ORIGIN      
        1 gccggtaccc ggagcgtgtc gacgtggccc gccgccggct cagccgtccg ctgaccttcg
       61 ccgagaagat cctcgtggcc cacgccgacg atcccgagac ggtcggcctc gaacggggcg
      121 tcgactactc gaactaccgg cccgaccggg tcgcgatgca agacgccacc gcgcagatgg
      181 ccctgctgca gttcatgacg gccaatctgg cgcaggtggc ggtccccacc accgtgcact
      241 gcgatcacct catccaggcg cacatcggag cggcacccga cctcaagacc gcactcgaca
      301 cgaactcgga ggtgtacgag ttcctgcgca gcgtctcgtc gaaatacggg atcgggttct
      361 ggaagcccgg ctccgggatc atccaccagg tcgtcctcga gcagtacgcg ttcccgggcg
      421 ggatgatgat cggcaccgac agccacacac cgaacgccgg tgggctcggc atggtcgcga
      481 tcggcgtcgg cggcgcggac gcagtcgatg tgatggcggg ctggccgttc aacacccggg
      541 tgccccggct catcggggtg cgactcctcg gccggctgtc gggttggacc gcggcgaagg
      601 acatcatcct gcgcgtcgcg gggatcctca ccgtcaaagg cggcaccggg gccatcgtcg
      661 agtacttcgg gcctggcacc gaatcgatct cggcgaccgg gaaggcgacg atctgcaaca
      721 tgggggccga gatcggcgcg acctgctcgc tgttccctta cgacgtccgc tcggcgcact
      781 acctgacagc caccggacgg gacgagatca gcggactcgc cgacgagtat gccgagttcc
      841 tgcgaggcga ccctgatgtc gaggccgacg cggagcggtt ctatgaccgg gtgatcgaga
      901 tcgatctcga cgacttggag ccccacatcg tcggcccgca cacccccgat ctcgaccggt
      961 ccctcgacga gctggtgcgg gtcgcgcccg aggagcggta cccgctcgac atctcgtacg
     1021 cgctggttgg gtcgtgcacg aactcgtcct acgaggacat cggccgagcg gcgcacgtcg
     1081 cccgccaagc cagcgccgcc ggcctgaagg tccggtcacc gctgctgatc acacccggat
     1141 ccgagcaggt ccgggcaacg atcgaacgcg acggcctgct cgacgacctg gaggccatcg
     1201 gcgccaccgt cctcgccaac gcctgcggac cgtgcatcgg acagtggcag cgcgacgaca
     1261 tcaagccggg ggagcgcaac accatcgtca gctcgttcaa ccgcaacttc ccgcgccgaa
     1321 acgacggcaa cgccgaaacg ctgtcgttca tcggatcacc cgagacagtg gtggcgatgg
     1381 cgctgaccgg acgactcgac cacgacttca tccgcacgcc gatcgaagcg cccgacgggc
     1441 accaggtgac gctcgaggct cccgccgccg acgagctgcc cgcgaaaggc ttcgacccgg
     1501 gccagtccgg cttcgtgcct cccgccgcgg tcggcgacga ggtcaacgtc gtcgtgaaac
     1561 cggattcgga gcgcctcgag ctgctggaac cgttcccgcc gtgggacggt caggacctgc
     1621 tcagtctgcg ggtgctcatg aaggccgtcg ggaagtgcac gaccgaccac atctcgcccg
     1681 cggggccctg gctgcgatac cgcggtcacc tggacaacat ctcgaagaac ctgttcactg
     1741 gcgtcaacaa cgcgttctcg ctcgacgagc ccggcaccgg gatcgacgga cgcgacggca
     1801 gcacccagcc gcttcccgat ctggcccgct cctaccgcga cgctgggatc gtctgggtcg
     1861 cggtcggcga cgagaactac ggcgaagggt cgtcgcgcga gcacgcggcg atggaacccc
     1921 gccatctgaa cggccgggcg atcatcgtcc ggtcgttcgc tcggatccac gagacgaacc
     1981 tgaagaagca gggggtgttg gccctgacgt tcgccgaccc cgacgactac gagcggatcc
     2041 tcgtcgacga caccatcgac atcgtgggcc tgggcgacct cgcgcctgac cggccggtca
     2101 cggtccgcat ccaccacgcc gatgggcgaa gcaccgacat cacctgtcgc cacaccatgt
     2161 ccgacgagca catcggctgg ttccgagccg gttcggcgct caacgtcctc cgcggggacc
     2221 gcggttgatt cgacgcgtgc gggtcgccgg gctgctcagc gtggcggtca tcctggccgc
     2281 gtgcggcggt ggcagcagcg ggggcgcagc acaccgcgcc gcgtcgacga cgaccgcgac
     2341 gagcagctcg ggtggctcct cggtcaacat cgggatcatc tgctccacac cgaccgatgc
     2401 cgccgacgcg gtcgtcaacg cgtggatgtc aggcgaccgg gctgcggcgc ggcgatgcgg
     2461 cacgccgacg gcggtggcga cccttttcgg tcgcgccgcg ggtcccgaac gctgggtcct
     2521 ccagcattgc gcggggacgg tgtgctcgtt cacgcatcca gacgggacgg tgcaactgac
     2581 cgtgggggga tccgacgccg cggggtgggt tgtggcgcgg gtgcgcttcg ggcgctgacc
     2641 cgcgggtcag cgtgcgctga gcggcgccag ctgcgccgcg gtgggctcgg cgcagcagaa
     2701 ccagggctca ctgagccgag gaacccgggc cgcccgttcc tcgtcgacag cgacggggtc
     2761 gaggccgacg gcggcgcgca ccgcctggta gacggccggg gtcgagtcac cggcggcgag
     2821 gcgcgcctcg acgaccgcgc tcagctcacc ttggagggcg tccatcgccg ggtcggcgga
     2881 ccgccacgag tgactggccc gctcggggtc gtatccgtcg aggtgcgcca ccacgtcttg
     2941 atggtcgagg agcagcgacc cgggagggag gagcagacgg atcgtgtact ggactgggtc
     3001 gacgttgctg accaggtcgt ggcgtgccac gaactcgagg agcgcccgca tctgctcgag
     3061 ggtcgtccag ggggtgaacg ggagccacga gggtcgcacc tcgatcccgt gctcgcggag
     3121 cagagcgacc gcggtggcct catcggcgac ggtgtgaccc ttgtcgagcc ggcccagcgt
     3181 ggcgtcgtcg acgctctcga acgctgagac cacgaacacg caaccggcgg cggcgaactc
     3241 cggccacacc tcgggatggg ccagcacgtg ttcgaccttg acggtgcagt cgaagctcag
     3301 atcggggaac cggtcatgga ccgcgtggac cacccgtcgg gcgtgggcga acccgttcag
     3361 gaagtcgggg tccgcgaacg tcacgtgctc ggcgcccgcc gcgacccgct gctcgacgtc
     3421 ggcgagaacc gcatcgagcg ccacgatgcg gatccggccg tcgtacacga ccggtaccgg
     3481 gcagtgccgg cagcggtgca cgcacccccg gctcgcttcg accgacgcca ccagctgctc
     3541 ggtgtcaccg cggaccagcc gggcgtagcg gtcgagagcg ggaagcagct ctcgcgccgg
     3601 gcggggcgcg ccgccccggg cggcgtcccg gccgaggtgc acgacctgat cgccctcgtc
     3661 cccgtcgacc caggcgagga gggcctcgtc ggtctctccg gcgagggccc ggtccgcggt
     3721 ttcgaccatc gccgagccgt acaggccgta acacgccacg aggacctcgg gtcggcggcg
     3781 ccggacctcg tcgatcacct gacgggccag gcgggtcgcc gtgtgcatcg gcaccgagca
     3841 cgccacccgt tgcgcccagt cgagctggct cgggtcccac gcgtcgaccg agaggtcgag
     3901 ggcccggacc tcgtgtcccg cagcccgcag gcgcgccgcc ggtccggcga cacccagcgg
     3961 ctggtggccc agctcgtagc aagagacgag caggacgcgc acgagtcgag ggtaccggtg
     4021 cgtggccgac cggctcgccg tcgggtcagc ggatctcgat gtggagccga tcctgcgcgg
     4081 cgcggagggc ctgttcgacc gcggcgggtg ttgaggcccg tgcgaacaag aacccgagat
     4141 accggtcgcc ctccgggacg ggctcgaccg accggccgct cacgatggtg atctcaagtc
     4201 cgacgattcc cgggacgctc gtcgcctcgt cttggccctg caccccgacg agcgtcccgg
     4261 cgcgcgggat cggcagcatc atcaccccgg acgcgtcagg ggcgcgtcgg aggtcgtcga
     4321 tgggaaggcc gagggcgtgg cgcacgatga cctcctcgag gctgatcccg gccccgaggc
     4381 gaagagtgcg ggcgcacagc ccgccgatgg agcgggccgc gacctccacg acccaaaccc
     4441 ggtcctcgtc gacgcggacc tcggcgtgaa ccggcccttc ggtcaagccg agggcacggg
     4501 cggcgtcccc ggtgacccgc tccacgcgag cgaggacgtc ggcgggcagg cgcgacggag
     4561 tgacgtagat cgtctcctcg aagtagggcc cttcgagcgg gtcgggcttg tcgaagaccg
     4621 cgaggacggt gagctcaccg gcccgcagga gggcctcgac ggccacttcg acgccgggca
     4681 cgtactcctc gacgaggagc gacccgccac ccgcgatgcg ggccgcgcgc gtcgccgcgg
     4741 cgcgggcggc gcccgcgtcg tcggcccgga tgacaccccg gctgcccgag aggccggtcg
     4801 gcttcacgac gcacggccag cccacgtcgg ggaccgcgtc gggatcctcg aacaccgcgt
     4861 atcggggttg ggggacctcg gccgcggcca gcgcgtcacg catcgccgcc ttgtcgtggg
     4921 tccgggccac tgcggccggc gggttgtggg ggaacccgag ctgctcaccg gcccgggccg
     4981 cggcgagggt gccttggtcg tcgacggcga cgacggcgtc gacaggatga cgggcatcca
     5041 ggcgctcgat cacccgggcc gcttcgt
//
LOCUS       DATCCH010000412         8172 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_358940, whole genome shotgun sequence.
ACCESSION   DATCCH010000412 DATCCH010000000
VERSION     DATCCH010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 8172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8172
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358940"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      180..375
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            399..1322
                     /locus_tag="VIJ44_07935"
     CDS             399..1322
                     /locus_tag="VIJ44_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013283571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5165864.1"
                     /translation="MSVLTRRAWRASSWTARAGAAVAVLITLVVSGVATTTPASAISP
                     TAAGATRPKRIVSLSPTATEMLFAIGAGHQVVAVDDQSNYPKAAPRTKLSGFQPNVES
                     ITGYRPDLVVLSDGTVADSLRQLGIRVLVDPAANTLADSYRQLTELGAVTGNRAGATR
                     VIGSMKTDLRSLAASVPKRSPEPTVYYELDNTFFSATSKTFIGQILAMAGLKNIADQA
                     DSSGSGYPQLSQEYIITANPSFVFLADSKCCAQNAATVSTRPGWSRIAAVTGKHVVTL
                     DDDIASRWGPRVVQLLRTIVDATQGRQAKAA"
     gene            1319..2359
                     /locus_tag="VIJ44_07940"
     CDS             1319..2359
                     /locus_tag="VIJ44_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014440329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEY5165865.1"
                     /translation="MRTAAAHLKSRWVLAGLVAVAIALLVGVAVGPTSIPLGRIVTEV
                     TRHLPLIGGGHGLDHQQATIVWQLRLPRVVLALLVGAMLAGAGASYQGVFRNPLADPY
                     LLGAAAGAGLAVTIVIATAPGLSGGGLNPLPIAAFIGALGAVLVAYLVGRASGGGSTA
                     SLLLAGVAVASFLTAVQTYVQQQHADTIREVYSWILGRLTTAGWSDVLLLLPYFVVTS
                     VILLAHRRILDVLAVGDAEATTLGISATKARIIIVVTASFATAAAVSVSGLIGFVGII
                     VPHTVRLLTGPSYRTILPLSLLFGAAFLTFADLAARTVAAPGEIPIGLVTAFVGAPFF
                     ILVLRTSRLAPS"
     gene            2356..3144
                     /locus_tag="VIJ44_07945"
     CDS             2356..3144
                     /locus_tag="VIJ44_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013021516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5165866.1"
                     /translation="MSGIAAHGLVVEHGQRRVVDGVDLVVEASEWVTVVGPNGAGKTT
                     LLHVLAGIVTADAGTVEIDGRDAARLRTRERARLVALVPQVPVVPPAIRVLDYVLLGR
                     TPHLPFLGTETAADLARAHDTLALLGLAALAERRVETLSGGERQRVLLARALLQEPQV
                     LLLDEPTSALDIGHQQDVLELIDTLRHEHGLTVVSTLHDLTLAGLYADRLVLLAKGRV
                     VDRGSATEVLTPDRLRQHFGAHVTVLHGDDGPVVIPHRTARSVP"
     gene            3141..4085
                     /locus_tag="VIJ44_07950"
     CDS             3141..4085
                     /locus_tag="VIJ44_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HEY5165867.1"
                     /translation="MTSPAPTAPGNVGQRIVSLLPSATEIVYALGLDDALVGVTFECD
                     YPADARTKRVVSDTSLPPGLSPAEIDQAVTERTDAHESIYTLDEGALRALNPDLVLTQ
                     DLCAVCAVDVSEVTDALDHLGCHAHVLTLDPQTLDEVLTDIQTVADVTGRPERGRDCI
                     AALRDRLARVRAAVNGLPRPPVFVLEWTDPPFNAGHWLPDMVSVAGGEPVLARAGAYS
                     EAVTWPAIVAAAPDVVVVAPCGYGLAGTVELLDDRALRAELAATPAGTAGQVWAVDAS
                     AYFVRPGPRLVDGVEILAGILHPDAWSPPAPDVAARLR"
     gene            complement(4112..5512)
                     /locus_tag="VIJ44_07955"
     CDS             complement(4112..5512)
                     /locus_tag="VIJ44_07955"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY5165868.1"
                     /translation="MPSVGATRRRAARLLAVVVTVGTVVGATGAAAPAASARPKPAWQ
                     SPTLDGIVQARPLVVGDIVVVATENNSLYGLAVSDGHRVWGPRHVGAPVPLSALASVN
                     RSADGCGDIDPLGITGSPVADLTAAPPRVYAVAEVLAPGSRVPVHELFGVEATSGRVV
                     VVPTPIDPPAMTHPELEQQRAGLAFANGTVYVGFGGLYGDCGIYHGFVVAARADGSGI
                     VGFFEAASESGNAGGAVWAPTGVTIDASGRLYAATGNSLHPPSGSAIDNSDAVVRLDP
                     TSGSVLDVFQPSSWRDDNASDLDLGSTAPVLLSDGHLFQVGKQNRAYLLDPASLGGAD
                     HHTPLASLDLCPAFGGNATLGSSVYVACQDGVQQVLVDTTRPGLRHGWSTTGGADGPI
                     TVGAGLVWSVNRSAGTLDGLDPATGHLVAHHPVALDSSQHFPIVAVAAGHVLVEAGRR
                     VVAFTVTGRSTTTTSS"
     gene            complement(5505..7724)
                     /locus_tag="VIJ44_07960"
     CDS             complement(5505..7724)
                     /locus_tag="VIJ44_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020392240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY5165869.1"
                     /translation="MAALLDRLGRAAARHKWITIAAWIIAAVAVAVVAVAAGGKTVDV
                     FSIPGAQSQQATDLLNRRFPSRAGDTAAVVFAARPGATLRDPQLAAAVAASQTRLGGL
                     AHVTQVAGPATPGLGGAFESRDGTVAYANVQYNTKATQLPANAFDQLEAAAAPARQAG
                     LRVVYGGAVADAFNQQTTDSADAIGILVAVVILLVAFGSVIAMSIPIGTALFGLAVGL
                     SLIFALAAVTDIGTVAPTLATMIGLGVGIDYSLFILTRHRQNLAAGMTVIDSIGLANG
                     TAGQAVLFAGGTVVIAICGLALAGIPYVTWLGFTSAMVVLVMITAALTLIPALLAVAG
                     SHLNRVRTPRLRRSARGHDHHALTGAQSHGWERWARFMSRHRWPAVFASLAILLVLAA
                     PMLGMRLGQVDDGSAPRSTTQRQAYDLISQGFGKGANGPLVLAVSFPTPGDTKAATIV
                     DTAVTRVPGVAAVAPPQLSPTRDAAVIVVVPSSAPQSAATETLVDRLRASVLPTAVKG
                     TGAAAYVGGITAAYIDLGQRIQDRLPLFIGAVVTLSFLLLMMVFRSVFVPLKAAVMNL
                     LSIGASFGVIVAVFQWGWLKDVVGVSETTPIVAYVPMMMFAILFGLSMDYEVFLLSRI
                     REDYHETRDNLDSVINGLSVTARVITSAALIMISVFLGFVASPEPTVKMFGLGLAVAV
                     FVDATVVRLVLVPATMELLGDANWWVPGWLDRILPRIEIEEGAPPAPVAQPVATPGA"
     gene            complement(7843..>8172)
                     /locus_tag="VIJ44_07965"
     CDS             complement(7843..>8172)
                     /locus_tag="VIJ44_07965"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5165870.1"
                     /translation="PARARQALTAPGVTTVVAVIADEVVGFATVLSDGVLDAYLSALL
                     VAESHRGEGIGAGLLQTGYRSSGATRVDLLAADGTDDFYARFPHRRFSGFRLHPDAAL
                     DPTPGPA"
BASE COUNT         1206 a         2947 c         2857 g         1162 t
ORIGIN      
        1 gaccccggcg gctactgcga tccatcgtcc ctgacgcatg cgctggttcc ttccgcgagg
       61 cgccgctgcc cgggcgcctt tgtcgtcgag ttgggagaac ctaccaagcg gcgcgccgat
      121 caccgccatc gcgccggtcg ctccgccgcg acgccttcac cggtcggtgg cggagcggag
      181 tgattgacga aatccgcggc cgaggctaga catcgggccg tcgtagtgaa cgggaagccg
      241 gtgcaagtcc ggcgcggtcc tcgccactgt gaccggggag cgaactcctt gttgccactg
      301 gacctcacgg tctgggaagg cggagtgagc actgatccgg gagccaggag accggctgcg
      361 gcaccttgac aggttcaatc cacgaaggat gggtgcagat gtctgtgttg acgcggcgcg
      421 cttggcgcgc atcgagttgg accgcgcgcg ccggcgcggc ggttgccgtg ctgatcacgc
      481 tcgtggtgtc gggcgtcgcg acgacgacgc cggcgtcggc gatatcgccg accgcggcgg
      541 gcgcgacgcg gccaaagcgc atcgtgtcgc tgtcgccgac cgcgaccgag atgctcttcg
      601 cgatcggcgc cggccatcag gtcgtcgcgg tcgacgatca gtcgaactat ccgaaggcgg
      661 cgccgcgcac gaagctctcg ggcttccagc ccaacgtcga atcgatcacc gggtatcgac
      721 ccgacctcgt cgtcctctcc gatggcaccg tcgccgattc gcttcgtcag ctggggatcc
      781 gggtgctggt cgacccggcc gcgaacactc tcgccgacag ctaccggcag ctgaccgagc
      841 tcggtgcggt gaccggcaac cgagccggtg cgacacgggt gatcgggtcg atgaagactg
      901 acctgcgcag cctggcggcg agcgtcccga agcgttcacc ggagccgacg gtgtactacg
      961 agctcgataa cacgttcttc tcggcgacgt cgaagacgtt catcggccag atcctggcca
     1021 tggccgggtt gaagaacatc gccgaccagg cggacagcag cgggtcgggc tatccgcagc
     1081 tgtcccagga gtacatcatc accgcgaacc cgagcttcgt cttcctggcc gacagcaaat
     1141 gctgcgcgca gaacgccgcg acggtgtcga cccgacccgg ctggtcccgg atcgcggcgg
     1201 tgaccggcaa acatgtggtc accctcgacg atgacatcgc gtcgagatgg ggtccgcggg
     1261 tcgtgcagct gttgcgcacg atcgtcgacg ccacacaggg gaggcaggcg aaagcggcgt
     1321 gaggaccgcg gccgcgcacc tcaagtcccg atgggtgctg gccggtctgg tcgcggtggc
     1381 gatcgcgttg ctcgtcggcg tcgcggtcgg tccgacgagc atcccgctcg gccggatcgt
     1441 caccgaggtc actcgtcacc tgccgctgat cggcggcggc catgggctcg atcatcagca
     1501 agcgacgatc gtgtggcagc ttcggctgcc gcgggtggtg ctggccctgc tggtcggggc
     1561 gatgctggcc ggggccggcg cctcgtatca gggggtgttc cgcaacccgc tcgccgaccc
     1621 gtacctgctc ggcgccgccg cgggcgcggg cctggccgtg acgatcgtca tcgctaccgc
     1681 gcccggcctc tccggcggcg ggctcaaccc gctgcccatc gccgcgttca tcggcgcgct
     1741 cggcgcggtg ctcgtcgcct acctggtcgg ccgggccagc ggcgggggat ccacggcgtc
     1801 gctgctgttg gccggggtcg ccgtcgctag cttcctcacc gccgtgcaga cctacgtcca
     1861 acagcagcac gccgacacca tccgggaggt ctactcctgg atcctgggac ggctcacgac
     1921 ggcgggctgg agcgacgtgc tgttgctgct cccgtacttc gtggtcacgt cggtgatcct
     1981 gctcgcccac cggcggatcc tcgatgtcct cgcggtcggc gacgccgagg caaccaccct
     2041 cggcatctcg gcgacgaagg cccgaatcat catcgtggtc accgcctcgt tcgcgacggc
     2101 cgcggccgtg tccgtcagcg ggttgatcgg cttcgtcggg atcatcgtgc cgcacaccgt
     2161 ccggctcctc accgggccga gctaccggac catcctcccg ttgtcgctcc tgttcggtgc
     2221 ggcgttcctc accttcgctg atcttgcggc ccggaccgtc gccgcgcccg gcgagatccc
     2281 gatcgggctt gtgaccgcgt ttgtcggcgc cccgttcttc atcctggtgc tacgcacctc
     2341 gcggctcgct ccgtcgtgag cggcatcgcg gcccacgggc tcgtcgtcga gcacgggcag
     2401 cgccgggtgg tcgacggcgt cgacctcgtc gtcgaggcca gcgagtgggt gacggtcgtc
     2461 ggaccaaacg gggcggggaa gaccacgctc ctgcatgtct tggcggggat cgtcaccgcc
     2521 gatgcgggga ccgtcgagat cgacggacgc gacgcagccc ggctccgcac ccgggaacga
     2581 gcccgcctcg tcgcgctcgt cccgcaggtg ccggtcgtac cgcctgccat ccgcgtcctc
     2641 gactacgtgc tgctcggccg aactccccac ctcccgttcc tcgggaccga gaccgcagcg
     2701 gacctggccc gagcccacga cacgttggcg ctcctcggcc tggccgcgct cgccgagcgc
     2761 cgagtcgaga ccctctccgg cggtgaacgc cagcgggtcc tcctggcccg cgcgctgctg
     2821 caggagccgc aggtgttgct cctcgacgag cccaccagcg cgctcgacat cggtcaccaa
     2881 caagacgtct tggagctcat cgacaccctc cgtcacgaac acgggctgac ggtcgtgtcc
     2941 accctccacg acctgacgtt ggcggggctg tacgccgacc ggctcgtcct gctcgccaag
     3001 ggacgcgtcg tcgaccgtgg ctccgccacc gaggtgctga ccccggaccg gctccgccag
     3061 cacttcggcg cgcacgtcac cgtcttgcat ggtgacgacg gacccgtcgt tatcccgcac
     3121 cgcaccgcgc gcagcgtgcc atgaccagcc ccgcgccgac cgcaccgggc aacgtcggac
     3181 agcggatcgt gtcgctgctc ccctcagcga ccgagatcgt ctacgcgctc gggctcgacg
     3241 acgccctcgt cggagtcacg ttcgaatgtg actatcccgc cgacgcccgc acgaaacggg
     3301 tggtgtcgga cacctcactg ccacccggcc tgtcaccggc tgagatcgac caagccgtga
     3361 ccgagcgcac cgacgcgcac gagtcgatct acaccctcga cgaaggggcg ctgcgcgccc
     3421 tcaaccccga tctcgtcctc acccaggatc tgtgcgcggt gtgcgcggtg gacgtgagcg
     3481 aagtcaccga cgccctcgac catctcggct gtcacgccca cgtcctcacc ctcgaccccc
     3541 agacgttgga cgaggtgctc accgacatcc agaccgtcgc cgacgtcact ggacgacccg
     3601 agcgcggccg cgactgcatc gcggcgctgc gcgaccgcct cgcccgggtc cgcgccgcgg
     3661 tcaacggcct cccccgtccc ccggtgttcg tgctcgaatg gaccgacccg ccgttcaacg
     3721 ccggtcactg gctcccggac atggtgagcg tcgccggcgg tgagccggtg ctggcccgcg
     3781 ccggcgcgta ctccgaggcg gtcacgtggc cggcgatcgt cgccgccgcg cccgacgtcg
     3841 tcgtcgtcgc accgtgcggc tacgggctgg ccggcaccgt cgagctcctc gacgatcgcg
     3901 ccctccgcgc cgagctggcg gccaccccgg ccgggaccgc ggggcaggtc tgggccgtcg
     3961 acgcgtcggc gtacttcgtt cgacctggcc cccgcctggt cgacggcgtc gagatcctcg
     4021 ccgggatcct gcaccccgac gcttggtcac ctcccgcccc ggatgtcgcc gcgcggttgc
     4081 gctgagactc gcggccggcg cccacgccgg actaggagga ggtggtggtg gtcgagcgtc
     4141 cggtcaccgt gaacgccacg actcgacgtc ccgcctccac aaggacgtgt cccgccgcga
     4201 ccgccacgat cgggaagtgc tgactcgaat cgagggccac gggatggtgc gccaccaggt
     4261 gcccggtggc cgggtccagg ccgtcgaggg tccctgcgga ccggttcacc gaccagacca
     4321 gcccggcacc gacggtgatg ggaccgtcgg cgccgccggt ggtgctccag ccgtgccgca
     4381 acccgggtcg tgtggtgtcg acgagcacct gctgcacccc gtcctggcag gccacgtaca
     4441 ccgacgagcc gagcgtcgcg ttcccgccga acgcgggaca caggtccagt gacgccagcg
     4501 gggtgtgatg gtcggcgccg ccgagcgagg ccgggtcgag caggtaggcg cggttctgct
     4561 tcccgacctg gaagagatgc ccgtcgctga ggagcaccgg cgcggtcgag cccaggtcga
     4621 ggtcgctggc gttgtcgtcg cgccacgagc tgggctggaa gacgtccagg accgagccgc
     4681 tggtcgggtc gaggcggacc acggcgtcgc tgttgtcgat cgccgacccc gacggggggt
     4741 gcagcgagtt accggtcgcg gcgtagaggc gacccgacgc gtcgatggtg acgcccgtcg
     4801 gtgcccacac cgcaccgccg gcgttgccgg actcgctcgc cgcctcaaag aacccaacga
     4861 tgccactccc gtcggcgcgg gccgcgacca cgaaaccgtg atagatcccg cagtcgccgt
     4921 ataggccgcc gaacccgacg tacaccgtcc cgttcgcgaa cgcgagcccg gcccgctgct
     4981 gctcgagctc ggggtgggtc atcgcgggcg ggtcgatcgg tgtcgggacc acgacgacac
     5041 gccccgaggt ggcctcgacc ccgaacagct cgtggaccgg cacccgactg ccgggggcca
     5101 ggacctcggc gacggcgtag acccgcgggg gcgcagccgt gaggtcggcg accggtgagc
     5161 cggtgatgcc gagcgggtcg atatcgccgc agccgtcggc ggaccggttg acggacgcca
     5221 gcgccgacag cgggaccggc gcgcccacgt ggcgcggccc ccagactcga tgcccgtcac
     5281 tcaccgcgag cccgtacaag gagttgttct cggtggcgac gaccacgatg tcgccgacca
     5341 ccaacggtcg ggcctggacg atcccatcga gggtcggcga ctgccacgcc ggcttcggcc
     5401 gcgccgacgc cgcgggcgca gcggcgccgg tcgcccccac gaccgtcccg accgtgacga
     5461 ccacggcgag gagccgggcg gcgcgccgcc gggtcgctcc gacgctaggc accgggtgtc
     5521 gcgaccggct gcgccaccgg cgctggcgga gcgccttcct cgatctcgat gcggggcagg
     5581 atacggtcga gccagcccgg gacccaccag ttggcgtccc cgaggagctc catcgtcgcc
     5641 ggcaccagca ccaggcggac caccgtcgcg tcgacgaaca ccgcgacggc gagaccgagc
     5701 ccgaacatct tcaccgtcgg ctcgggactg gcgacgaagc cgaggaacac gctgatcatg
     5761 atcagcgccg cggaggtgat gacccgcgcc gtcaccgaga gaccgttgat gacactgtcg
     5821 aggttgtctc tggtctcgtg gtagtcctca cggatccgcg acagcaggaa cacctcgtag
     5881 tccatcgaca atccgaacag gatggcgaac atcatcatcg gcacgtaggc cacgatcggc
     5941 gtcgtctccg acacgccgac gacatctttg agccagcccc attggaacac cgcgacgatc
     6001 accccgaacg acgcgccgat cgacagcagg ttcatcactg cggccttcaa cgggacgaac
     6061 accgaccgga acaccatcat cagcaacagg aacgacaagg tgacgaccgc gccgatgaac
     6121 agcggcaaac ggtcctggat ccgctgaccg agatcgatgt acgcggcggt gatgccaccg
     6181 acgtaggccg cggctccggt gcccttcacc gcggtgggta gcacgctggc ccgcagacgg
     6241 tcgacgagcg tctcggtcgc cgcggactgc ggcgctgatg acgggacgac gacgatgaca
     6301 gccgcgtccc gagtcgggct gagctggggc ggggcgaccg ccgccacgcc gggtactcgc
     6361 gtcaccgcgg tgtcgacgat ggtggccgct ttcgtgtcgc ccggcgtcgg gaacgacacg
     6421 gcgaggacca gcggaccgtt ggcacccttt ccgaatcctt ggctgatgag gtcgtacgcc
     6481 tggcgttggg tggtgctcct cggcgccgac ccgtcgtcga cctgtccgag gcgcatcccg
     6541 agcatcgggg ccgcgaggac caacaagatg gcgaggctgg cgaacaccgc gggccaacgg
     6601 tgccgcgaca tgaaccgggc ccatcgctcc cacccgtgac tctgcgcccc ggtgagcgca
     6661 tggtggtcgt ggccacgcgc cgaccggcgc agccggggag tccgcacccg gttgagatgg
     6721 gaccctgcga cggcgaggag cgcggggatc agggtcagcg cggcggtgat catgaccagc
     6781 acgaccatcg cggaggtgaa cccgagccac gtgacatacg ggatcccggc gagcgccaac
     6841 ccgcagatcg cgatgacgac cgttcccccg gcgaacagca ccgcctgacc ggcggtcccg
     6901 ttcgcgagac cgatcgagtc gatcaccgtc atgcccgcgg cgaggttctg gcgatggcgg
     6961 gtgaggatga acagcgagta gtcgatcccg acgccgaggc cgatcatcgt ggcgagggtc
     7021 ggcgcgaccg tcccgatgtc ggtcaccgcg gccagggcga agatcagcga gagcccgacc
     7081 gccaacccga acagggcggt gccgatcggg atgctcatcg cgatcacgga gccgaacgcg
     7141 accagcagga tcacgaccgc cacgaggatg ccgatggcgt cagcgctgtc ggttgtctgc
     7201 tggttgaacg catcggcgac agccccgccg tacacgaccc gcagcccggc ctgacgggcg
     7261 ggggccgccg cggcctccag ctgatcgaac gcgttcgcgg gaagctgggt ggccttcgtg
     7321 ttgtactgga cgttggcgta cgcgacggtc ccgtcgcggg actcgaacgc tcctccgagc
     7381 cctggcgtcg cggggccggc gacctgggtg acgtgggcca gcccgcccag gcgggtttgg
     7441 gaggcggcga ccgcggccgc cagctgcggg tcgcgcagcg tcgcgccggg ccgcgccgcg
     7501 aacacgaccg cggcggtgtc gccggcgcgg cttgggaacc gccggttgag gaggtcggtc
     7561 gcctgttgcg attgggcgcc cgggatcgag aacacgtcga cggtcttccc gccggccgcg
     7621 accgcgacga cggcgaccgc gacggccgcg atgatccagg cggcgatggt gatccacttg
     7681 tgacgggccg cggcgcggcc cagtcggtcc aacagtgcag ccacgtggcc tccctatcgt
     7741 cgcccgctga ccggcgagcg tgtccgcggt cccaccacgt cgaccattcg tcccgccggg
     7801 tcgaggccgg taccgggctc gaggcccggg ccgggcacca tgtcaggccg ggcccggcgt
     7861 cgggtcgagc gccgcgtccg ggtgcagccg gaagcccgag aagcggcggt gagggaaccg
     7921 ggcgtagaag tcgtcggtgc cgtcggcggc caagaggtcg acccgcgtcg cgccgctcga
     7981 gcggtacccg gtctggagca ggccggcgcc gatcccctca ccgcggtggg actcggcgac
     8041 gagcaacgcc gagagatacg cgtcgaggac cccgtcgctg agcaccgtcg cgaatccgac
     8101 cacctcgtcg gcgatgacgg cgaccacggt ggtgacgccc ggcgcggtga gcgcctggcg
     8161 cgcccgggcg gg
//
LOCUS       DATCCH010000413         2751 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_446256, whole genome shotgun sequence.
ACCESSION   DATCCH010000413 DATCCH010000000
VERSION     DATCCH010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446256"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VIJ44_07970"
     CDS             <1..231
                     /locus_tag="VIJ44_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019180510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY5165871.1"
                     /translation="VVDDDGAGVPPEERDRVFERFTRLDEGRARDGGGAGLGLAMVHA
                     IAERHGGTVEVGDAPIGGARFVVRLPAGSVAP"
     gene            245..991
                     /locus_tag="VIJ44_07975"
     CDS             245..991
                     /locus_tag="VIJ44_07975"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEY5165872.1"
                     /translation="MAELTLVTFNTHYGLRSLHDHATPYDLGAVLVDLAADAMVIQEV
                     WRPDGIRGAVDDFADTHGYTMHHVVTGRATCLRRRWPHHHPDGEGTIGTAILTRVPAQ
                     RRPDVLVGPTFGDPAPRRAVVHVEVGDDDDAAPLQLVGVHLTSRLPHGPPLQLRRLAR
                     ALPGRGTAAVVAGDCNFWGPPVVMLLADGWRRTVTGRTWPAGRPHSQIDQVLVRPGDV
                     RVLDAEVGADVGSDHRPLRVRVGWPNASGS"
     gene            complement(988..1611)
                     /locus_tag="VIJ44_07980"
     CDS             complement(988..1611)
                     /locus_tag="VIJ44_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165873.1"
                     /translation="MSPARRAVRDDLTRLGKRTVTGPSFGFSARLERRLVGGVEALRG
                     VTLLALPRRQRRRLPAVTVAAASVAGVVLAGALLGAFGHGGTGALQLAAAVDTTVVMP
                     GGQTVPGRTGLGLPDGSVVWTGPNGRAAAGPVELGPGIEGFVDRGQLRLQSLPAGSGP
                     SNLAPVTTPPTPSVVHVPAGHVPKVTVPTVPSVGSPTTLALHRGDRH"
     gene            complement(1608..2219)
                     /locus_tag="VIJ44_07985"
     CDS             complement(1608..2219)
                     /locus_tag="VIJ44_07985"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY5165874.1"
                     /translation="MGPPNPEPWQPSRAAPASFDERALIARARSDPSAFGVLYRAYID
                     RIYAFAYRRTWTREAAEDVTAATFERALRHLERFDVDGAGFGPWLFRIAANELVDHYR
                     REGRTRTDRGQRGLHVLAGHAVEDNVDAIEREDEVRDMIEALGTLRPRYQRALTLRYL
                     ADLSADEAATAMGCSKPVLAVTLHRALGALRRAVEPDSSDPDA"
     gene            2447..2638
                     /locus_tag="VIJ44_07990"
     CDS             2447..2638
                     /locus_tag="VIJ44_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEY5165875.1"
                     /translation="MRVRVDAEACTGHGRCYSLAPDLFDADDVGHCVLVHEEVPEGLE
                     DQARVGVQNCPEQAISVED"
BASE COUNT          369 a          957 c         1051 g          374 t
ORIGIN      
        1 gtcgtcgacg acgacggcgc cggggtgcca cccgaggagc gtgaccgggt cttcgagcgg
       61 ttcacccgcc tcgacgaggg tcgcgcccgc gacggtggtg gcgccggcct cgggttggcg
      121 atggtgcacg ccatcgccga acgtcatggc ggcaccgtgg aggttggcga cgcgccgatc
      181 ggtggtgccc ggttcgtggt ccgcctccct gcggggagtg tcgccccgta gggttccggc
      241 ccgcatggcc gagctgactc tcgtcacgtt caacacgcac tacggattgc ggtcgctgca
      301 cgaccacgcg acgccgtatg acctcggggc cgtgctcgtc gacctcgccg cggacgccat
      361 ggtcatccaa gaggtgtggc gacccgacgg gatccgaggc gcggtcgacg acttcgccga
      421 cacgcacggg tacacgatgc accacgtcgt gaccggccgg gcgacgtgtc tccggcggcg
      481 gtggccgcac catcatcccg acggtgaagg aacgatcggc accgcgatcc tcacccgggt
      541 cccggcccag cggcgtcccg acgtgctcgt cggcccgacc ttcggtgacc cggcgccccg
      601 gcgggcagtc gtgcacgtcg aggtcggcga cgacgacgac gccgcgccgt tgcagctggt
      661 gggtgtgcac ctgacgtcgc gtctgccgca tggtccgccc ttgcagctcc gacgactggc
      721 ccgcgcgctc ccgggacgcg ggaccgcggc ggtggtcgct ggcgactgca acttctgggg
      781 gccaccagtc gtgatgctgc tcgccgacgg gtggcgacgg acggtgacgg gacggacgtg
      841 gccggcgggt cgaccgcaca gccagatcga ccaggtgctc gtccgccccg gtgatgtccg
      901 ggtcctcgac gccgaggtcg gcgccgacgt cggctccgat caccggccgc ttcgggtccg
      961 ggtcgggtgg ccgaacgcgt cgggctctta gtgccgatcg ccccggtgaa gcgccagcgt
     1021 ggtcggcgag cccaccgacg gcaccgtcgg gacggtcacc ttcggcacgt gaccggccgg
     1081 cacgtggacc accgacgggg tcgggggtgt cgtcaccggc gccaggttgc tcgggccgct
     1141 gccggcggga agtgactgga gtcgcagctg cccacggtcg acgaaaccct caatgccggg
     1201 accgagctcg accgggccgg ccgcggcccg cccgttgggt ccggtccaca cgacggagcc
     1261 gtctgggaga ccgagaccgg ttcggcccgg gacggtctgc ccgccgggca tgacgaccgt
     1321 ggtgtcgacg gccgcggcga gctggagggc gccggtgccg ccgtgaccga acgcgccgag
     1381 gagcgcgccg gcgagcacga ccccggcgac gctcgccgcg gcgacggtga ccgccggcag
     1441 ccgtcgccgc tgacggcgcg gcagggcgag cagcgtcacc ccacggagcg cttcgacgcc
     1501 gccaacgagt cggcgctcga ggcgggcgct gaacccgaac gacggtccgg tgacggtccg
     1561 cttcccgagt cgggtgagat cgtctcggac cgcgcgtcgg gcgggactca tgcgtccggg
     1621 tccgacgagt cgggctccac cgcgcggcgc agcgcgccga gggcccggtg caaggtgacc
     1681 gcgaggaccg gcttcgagca ccccatggcg gtggcggcct cgtcggcgga gagatcagcg
     1741 aggtagcgca gggtgagcgc gcgctggtaa cggggacgaa gggtcccgag cgcctcgatc
     1801 atgtcgcgga cctcgtcttc gcgttcgatg gcgtcgacgt tgtcctcgac cgcgtggccg
     1861 gcgaggacgt gcaggccccg ttggccgcgg tcggtgcggg tgcgtccttc gcgtcggtag
     1921 tggtcgacga gctcgttggc cgcgattcgg aacagccacg gaccgaaccc ggcgccgtcg
     1981 acgtcgaagc gctcgaggtg gcgcagcgcc cgctcgaagg tggccgcggt gacgtcctcg
     2041 gcggcctcgc gcgtccaggt gcgacggtac gcgaaggcgt agatccgatc gatgtaggcg
     2101 cggtacagaa ccccgaacgc cgaggggtcc gagcgggccc gggcgatgag cgcccgctca
     2161 tcgaaggatg ctggcgctgc cctcgacggt tgccacggtt ctggattggg cggccccata
     2221 gccccagaac gctaggcgca gccaccaggt ccgtcgagca accggctacg tgacggccga
     2281 ggccgatgac gccgggggca gggatggggc aggcggcgcc accggccccg gctcgaccgg
     2341 ggtcgtgggt acgacgacag ggatcggcat gcatcgggga caacgacgtc cggccgcacc
     2401 gatttcacgt tctgccccac gatttctggc cggtaccctg ccggcgatgc gggtgcgggt
     2461 cgacgccgag gcgtgcacag gtcacggtcg ctgttacagc ctcgcccccg acctgttcga
     2521 cgccgacgac gtcggccatt gcgtcctcgt gcacgaggag gtccccgagg ggctcgagga
     2581 ccaggctcgc gtcggggtcc agaactgccc cgagcaggcg atcagcgtcg aggactgacc
     2641 ccggcccacg gccgcggcgg ggcttgtacc gtgcggcgtc gtggactcct cggctccgat
     2701 gaatggcgcg acggtggtgg tcactggcgc caacgccggc gtcggcctcg a
//
LOCUS       DATCCH010000414         3886 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_446775, whole genome shotgun sequence.
ACCESSION   DATCCH010000414 DATCCH010000000
VERSION     DATCCH010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446775"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..1189
                     /locus_tag="VIJ44_07995"
     CDS             182..1189
                     /locus_tag="VIJ44_07995"
                     /inference="COORDINATES: protein motif:HMM:TIGR03544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEY5165876.1"
                     /translation="MASMDVTPQELRDVEIREAWRGYHRDDVDELLERAAATIEHLEE
                     EVHSAQSRATQAPTPPPVRTPAPVAPTPAPAPERRPSGIDTDVIQRTLVLAQKAADEA
                     VHEARAKAQQMLVESEAKAQSLVSEAEASARRIADTERRRLEEEIGQLTSARDTLNAD
                     VDALERYEQEYRIRLREAIHAELETLENAAHGTSDRPSLHAVQVPAARSWSASAVPET
                     WSGASEGVTPVPPEPSAALPSAGSPTVNIQAVPAHEQWDSDPRGNWSETPTAAVVDDS
                     SEHGLPEHEGDSLDDDAFFASLREAVRDDTPLSDREPTGEQYLDTAEGDDQRKLFRRR
                     R"
     gene            complement(1186..>3886)
                     /gene="ileS"
                     /locus_tag="VIJ44_08000"
     CDS             complement(1186..>3886)
                     /gene="ileS"
                     /locus_tag="VIJ44_08000"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEY5165877.1"
                     /translation="GMWLDTANAYWTLTNDYVESVWWHLRQLWDAGSIYEGFKVVPYC
                     GRCGTALSSHEVAQGYEDVTEPSVYVRFPLVDDDRDLLVWTTTPWTLVSNVAAAVGRE
                     LRYVHVRGGDGRRDLVLAAARVGPVLGDDVEIVGDVPVEELIGRRYQRPFEVLDVPGD
                     ACRVVAAEFVTVDDGSGIVHLAPAFGEVDRQVAERAGLPVLNPVDAEARFDTSVSAYA
                     GRFVKDVDPLILDDLSARGLVERVVDYTHAYPHCWRCGTPLIYWAKPTWFVRTSEHRD
                     ELLRQNEGVGWHPEHIKHGRFGDWLENNVDWALSRDRYWGTPLPFWRCAGGHDTCVGS
                     VAELAELAGRDLSGLDLHRPEVDDVTIRCPVCEGRARRVEPVLDAWFDSGVMPASQFH
                     FPFENAETFERRFPADFVCEAIDQTRGWFYSLLAANTLVFGRAPYRNVVCLAHILDRD
                     GQKMSKSRGNVIDPWTILRSQGADALRWYFFSAGSPWTNRRVDERTIDESTRRFLLTL
                     WNTASFFVTYANLDGWEPGPPVAAEHVLDRWIRSRLASTVREVTDALEDFDALRGAQA
                     LEGLVDDLSNWYVRRSRARFWKSSDPLASATLHECLVTVTRLLAPFCPFTADELHRVV
                     APSRQSVHIGDWPDVDDRAYDAELESGMALARQLVTLGLAARNEAKLKVRQPLRRALV
                     LLRPGVELPTELAAQVAEELNVRVLEPVHDLEGLLDYTVVPDFRRLGPRIGPLLPRLK
                     QALADADGAALRRALATDGRVVVDLGGEQVELGPDDVEVRAHAHEEFVLAEDRDLAVA
                     LDTTVDDELRAEGLARGLVRTLNDHRKAQGLDLADRVRVELRASGAIADAAQRHTAWI
                     AEEVLATSFKVETPANGPRDGFALLEVDGVPVRVRLERDDSDA"
BASE COUNT          678 a         1436 c         1254 g          518 t
ORIGIN      
        1 ccggctgctc atcgagctca ccgactgggc cgtccgaccc tttcgtcgca tcatcccgcc
       61 ggtcgggatg ttcgacatct ccttcatgat cctcgtgttc ttcctgttga tcttccgcac
      121 tgccatcggc tgctgacgcg gcatcgcctc ggccgggacc gcatcgctag cattgacccc
      181 tatggcttcc atggatgtca ccccgcagga gctgcgcgac gtcgagattc gtgaagcgtg
      241 gcggggctac caccgcgacg acgtcgacga gctgctcgaa cgcgccgcgg cgacgatcga
      301 gcacctcgaa gaggaggtcc actcggcgca gagccgcgcc acgcaggcac cgaccccccc
      361 gcccgtccgc accccggctc cggtcgcgcc gacaccggca ccggcaccag aacgccggcc
      421 ctccggcatc gacaccgacg tcatccaacg gaccctcgtc ctcgcccaga aggccgccga
      481 cgaagcggtc cacgaagctc gcgccaaagc ccagcagatg ctcgtcgaat ccgaagcgaa
      541 agcccagtcg ctcgtaagcg aggccgaagc gagcgcgcgc cgcatcgcgg acaccgaacg
      601 gcgccgcctc gaggaggaga tcggccaact cacctcggcg cgagacacat tgaacgccga
      661 cgtcgacgcc ctcgagcgct acgagcagga gtaccggatc cgcctccgcg aggcgatcca
      721 cgccgagctc gaaaccctcg agaacgctgc tcatggaacc agtgaccggc cctccctgca
      781 cgcggtccag gtgccggccg cccgctcgtg gtcggcgtcg gccgttcccg aaacctggtc
      841 gggcgcgtcc gaaggcgtga caccggtgcc gcccgaacca tcggccgcgc tgccgtcggc
      901 ggggagcccg accgtgaaca tccaagcggt gcccgcccac gagcagtggg attccgatcc
      961 ccgcggcaac tggagtgaaa cccccaccgc ggcggtggtc gacgactcat ccgagcacgg
     1021 gctccccgaa cacgaaggtg acagcctcga cgacgacgcg ttcttcgcgt cgctgcgtga
     1081 agcggttcgc gacgacacgc cgctcagcga ccgtgaaccc accggcgagc agtacctcga
     1141 caccgcggaa ggcgacgatc agcggaagct cttccgccgc cggcgctaag cgtcggagtc
     1201 gtcgcgctcg agccgcaccc gcaccggcac gccgtccacc tcgaggagcg cgaagccgtc
     1261 gcggggcccg ttggctgggg tctcaacctt gaacgacgtc gccagcacct cctcggcgat
     1321 ccatgccgtg tggcgctggg cggcgtcggc gatcgcaccg gaggcgcgga gctcgacccg
     1381 gacccggtcg gcgaggtcga ggccctgcgc cttgcggtga tcgttgagcg tacggaccag
     1441 cccgcgcgct agaccctcgg cgcggagctc gtcgtcgacg gtcgtgtcga gcgcgaccgc
     1501 caaatcgcgg tcttcggcga ggacgaactc ctcgtgggcg tgggcacgca cttccacgtc
     1561 gtcggggccc agctccacct gctcgccccc gaggtccacc acgacacgtc cgtcggtcgc
     1621 cagcgcccgg cgcagcgccg cgccgtcagc gtcggcgagc gcctgcttca agcggggcag
     1681 caacgggccg atccgaggtc cgagccggcg gaagtcgggc accacggtgt agtcgagcag
     1741 cccttcgagg tcgtgcaccg gctcgaggac ccggacgttg agctcctcgg cgacctgggc
     1801 tgccagctcc gtcggcagct cgacaccggg acggagcagc accagcgcgc gccgtaacgg
     1861 ttggcgcacc ttcagcttgg cctcattgcg agccgcgagg ccgagggtga cgagctgacg
     1921 ggcgagcgcc atgccggact ccagctccgc gtcatacgca cggtcatcga cgtcgggcca
     1981 atccccgata tgcaccgact gccgcgacgg cgccacgaca cggtggagct cgtcggcagt
     2041 gaagggacag aacggcgcca gcagccgagt cacggtcacg aggcactcgt gcaacgtcgc
     2101 cgacgccagc gggtccgacg acttccagaa ccgggcccgt gaccgacgca cgtaccagtt
     2161 cgacaagtcg tcgaccaggc cctcgagtgc ctgggcgcct cgcaacgcgt cgaagtcctc
     2221 gagggcatcc gtcacctcgc ggaccgtcga cgccagccgc gaccggatcc aacggtcgag
     2281 gacatgctcc gcggcaaccg ggggccccgg ctcccagccg tccaggttgg cgtaggtgac
     2341 gaagaatgac gcggtgttcc acagcgtgag gaggaaccgg cgagtcgact cgtcgatcgt
     2401 ccgctcgtcc acccgacggt tcgtccacgg tgagcccgcg gagaagaagt accagcggag
     2461 cgcgtcggca ccctgggatc gcaggatggt ccacgggtcg atcacgttcc cacgcgactt
     2521 cgacatcttc tgcccgtcgc ggtcgaggat gtgagcgagg cacacgacgt tgcggtatgg
     2581 cgcccgcccg aacaccagcg tgttcgcggc gagcagcgag tagaaccagc cccgggtctg
     2641 gtcgatcgcc tcgcacacga agtcggcagg gaagcgccgc tcgaacgtct cggcgttctc
     2701 gaacgggaaa tgaaactgcg acgccggcat cacaccggag tcgaaccacg cgtcgaggac
     2761 cggctcgacg cgccgcgctc gtccttcgca caccgggcag cggatcgtca cgtcgtcgac
     2821 ctcggggcgg tgcaggtcca gcccggacag gtcgcgaccg gcgagctccg cgagctccgc
     2881 gaccgaccct acgcaggtgt cgtggccccc agcgcagcgc cagaacggca ggggcgtccc
     2941 ccaataccgg tcccgagaca gcgcccagtc gacgttgttc tccagccagt caccgaaccg
     3001 gccgtgcttg atgtgctcgg gatgccatcc gacgccctcg ttctgacgca ggagctcgtc
     3061 gcgatgctcc gacgtccgga caaaccacgt cggcttcgcc cagtagatga gcggcgtccc
     3121 acaccgccag caatgggggt acgcgtgggt gtagtcgacg acccgctcga cgagaccccg
     3181 agctgacaga tcgtcgagga tgagtgggtc aacgtccttc acgaaccgtc ccgcgtacgc
     3241 cgagaccgag gtgtcgaagc gcgcctcggc atccaccggg ttcagcaccg ggaggcccgc
     3301 ccgctcagca acctggcggt cgacctcacc gaacgcgggc gccagatgca caattcccga
     3361 cccgtcgtcg accgtcacga actcggccgc gacgacgcgg cacgcgtcgc cgggaacgtc
     3421 caagacctca aagggtcgct ggtagcgccg accgatcagc tcctcgacgg gcacgtcgcc
     3481 cacgatctcg acatcgtcac cgaggaccgg acccacccgt gccgcggcca ggacgagatc
     3541 ccgccgcccg tcgccgccgc gcacgtgcac ataccggagc tcgcgcccga cagccgcggc
     3601 gacgttcgac accagcgtcc acggggtcgt ggtccacacc aagaggtcgc gatcgtcgtc
     3661 gaccagcggg aagcgcacgt acaccgacgg ctccgtcaca tcctcgtagc cctgcgcgac
     3721 ctcgtgactc gacaacgcgg tcccacaccg gccgcagtac gggacgacct tgaagccctc
     3781 gtagatggaa ccggcgtccc aaagctgacg cagatgccac caaacgctct cgacgtagtc
     3841 gttcgtcaac gtccagtagg cgttcgcagt gtcgagccac atcccg
//
LOCUS       DATCCH010000415         2159 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_447108, whole genome shotgun sequence.
ACCESSION   DATCCH010000415 DATCCH010000000
VERSION     DATCCH010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447108"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1117)
                     /gene="secF"
                     /locus_tag="VIJ44_08005"
     CDS             complement(<1..1117)
                     /gene="secF"
                     /locus_tag="VIJ44_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009611379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEY5165878.1"
                     /translation="MSRLGDLGHHLYRGDVSYDFVGRRRRWYTISAAILVVSIAALLL
                     RGLNLGVDFSGGNVVTFPTSSGTVAQARAVADGIGAKDVTVTTVSSTSGRSIRVQTET
                     LDSTKAQALTDGLVREFNLDPTSVNVQAISAAWGSEITKKALTGLVVFLLLISLFLSL
                     YFEWRMAVGALVALAHDLVITVGVYALVGFTVTPATVIGVLTILGYSLYDTVVVFDKV
                     RENSRGLAAGNRMTYSQAANLAVNQTLVRSINTSIIALLPVASILFIGPLLGADTLKD
                     LALALFVGIGAGTYSSIFIATPVAAQLKEREPAMQALAKRVGAREAAGKVATGAVVGA
                     RGRSRAAARAAAAGTDSAVEDFDLEGGDGADGADGGVD"
     gene            complement(1117..>2159)
                     /gene="secD"
                     /locus_tag="VIJ44_08010"
     CDS             complement(1117..>2159)
                     /gene="secD"
                     /locus_tag="VIJ44_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEY5165879.1"
                     /translation="LVTCSTDGSEKFILGPELIPVSDISGASAGLLTTSTGATTGAWV
                     VNLTFNGTATSKFAQITRQMFAATNGSDANRFAIVLDAQVISAPTVNGVIADGRPQIS
                     GSFTQQTASDLANQLKYGALPLAFKSENLQTISPTLGSDQLRAGIIAGLLGLGLVVIY
                     SLFYYRGLGLVTVASLLVAGLLTFGSLVLLGHTMGYTLSLAGIAGAIVSIGITADSFV
                     VLFERIRDEMRDGRTLRVAVETGWKRAQRTILAADAVSLIAAVVLYLLAVGGVQGFAF
                     TLGLTTLIDVFVVFLFTKPLLTRLARTKFFGNGHRWSGVSADRLGVSRPTPSSRAAAR
                     KTPTTPTQPKEA"
BASE COUNT          372 a          725 c          761 g          301 t
ORIGIN      
        1 cgtcgacgcc gccgtcagcg ccatcagcac cgtcgccgcc ctcgaggtcg aagtcctcga
       61 ccgcgctgtc ggtaccggcc gccgccgccc tggccgctgc ccgcgaccgc ccgcgggcac
      121 cgacgacagc accggtcgcc accttgccag cagcctcgcg cgctcccacg cgcttggcga
      181 gggcctgcat cgcgggctcc cgctccttga gctgcgcggc gaccggggtc gcgatgaaga
      241 tcgaggagta cgtgccggcg ccgatgccga cgaacagcgc gagcgccagg tccttcagcg
      301 tgtcggcgcc cagcagcggc ccgatgaaca ggatcgaggc caccggcagc agcgcgatga
      361 tcgaggtgtt gatcgaccgc accaaggtct ggttgaccgc gaggttggcg gcctggctgt
      421 acgtcatgcg gttgccggcg gcgagaccgc gcgagttctc gcgcaccttg tcgaagacga
      481 ccacggtgtc gtacagggag tagccgagga tcgtcagcac gccgatgacc gtggccggcg
      541 tgacggtgaa ccccacgagg gcatagacgc ccaccgtgat gacgaggtcg tgcgcgagcg
      601 cgacgagggc gccgaccgcc atccgccact cgaagtacag gctgaggaac agcgagatca
      661 gcagcaggaa cacgaccaga cccgtcaagg ccttcttggt gatctcgctc ccccaggccg
      721 ccgagatcgc ctgcacattg accgatgtcg ggtcgaggtt gaactccctg acgaggccgt
      781 cggtgagcgc ctgcgccttg gtgctgtcga gggtctccgt ctgcacccgg atcgaccgac
      841 cgctcgtgga cgacaccgtc gtgaccgtga cgtccttggc cccgatgccg tcggcgaccg
      901 cacgggcctg cgcgacggtg ccgctggacg tcgggaacgt gaccacgttg ccgccggaga
      961 agtcgacgcc gaggttgagc ccgcgcagca gcagggccgc gatcgagacg accaggatcg
     1021 cggccgagat cgtgtaccac cgcctgcgcc ggccgacgaa gtcgtacgag acgtcgccgc
     1081 ggtagaggtg gtgaccgagg tcgccgagac gggacatcag gcctccttcg gctgcgtcgg
     1141 ggtggttggc gtcttgcgcg ccgcggcccg agaggacggg gtcggtcgtg acacgcccaa
     1201 ccggtcggca gagacacccg accagcgatg gccgttgccg aagaacttcg tgcgcgcgag
     1261 ccgggtcaac agaggcttgg tgaacaagaa caccacgaag acgtcgatca aggtggtgag
     1321 ccccagagtg aatgcgaagc cctgaacccc gccgaccgcg agcaggtaca gcacgaccgc
     1381 ggcgatcagc gatacggcgt cagcagccag gatcgtgcgc tgcgcccgct tccagccggt
     1441 ctccaccgcc acgcgcaagg tacggccgtc acgcatctcg tcccggattc gttcgaacag
     1501 cacgacgaac gagtcggccg tgatcccgat cgagacgatc gcacccgcga tgccggccag
     1561 ggacagcgtg tagcccatcg tgtgcccgag gagcaccaac gagccgaacg tgagcaggcc
     1621 ggcgaccagg aggctcgcca cggtcacgag gccgaggccc cggtagtaga acagcgagta
     1681 gatgaccacc agtccgaggc ccagcagccc ggcgatgatg cccgcgcgga gctggtcgct
     1741 gccgagcgtc ggcgagatcg tctgcaggtt ctcggacttg aacgccagcg gcagcgcgcc
     1801 gtacttgagc tggttggcga ggtcgctcgc ggtctgctgg gtgaagctgc cggagatctg
     1861 cggccggccg tcggcgatga ccccgttgac ggtcggagcc gagatcacct gggcgtccag
     1921 gacgatggcg aaccggttgg cgtcactgcc gttcgtcgcc gcgaacatct gccgggtgat
     1981 ctgggcgaac ttcgacgtcg ccgtgccgtt gaaggtcagg ttgacgaccc acgcgccggt
     2041 cgtcgcgccg gtgctggtgg tgagcaggcc ggcggaggcg ccactgatgt ccgagaccgg
     2101 gatcagctcg ggcccaagga tgaacttctc cgagccgtcc gtcgagcagg tgacgagct
//
LOCUS       DATCCH010000416         2019 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_447419, whole genome shotgun sequence.
ACCESSION   DATCCH010000416 DATCCH010000000
VERSION     DATCCH010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447419"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VIJ44_08015"
     CDS             complement(<1..221)
                     /locus_tag="VIJ44_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165880.1"
                     /translation="MFNSSGRAVVGAFAATRVAIGVAFVIAPGRLTRGSAATDTLMTR
                     TFAVREVVLGVGGLLAVAPADSRPSDVRL"
     gene            complement(506..790)
                     /locus_tag="VIJ44_08020"
     CDS             complement(506..790)
                     /locus_tag="VIJ44_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165881.1"
                     /translation="MSIVVLSETPNMTSKQYDRVAAELGLSNSLPEGCRAHIAGVGPD
                     GSTWRDVSVWDSPEQAKHFMDTTLRPAMERHGATPIWGPPTTWELHQLVT"
     gene            complement(1134..1799)
                     /locus_tag="VIJ44_08025"
     CDS             complement(1134..1799)
                     /locus_tag="VIJ44_08025"
                     /inference="COORDINATES: protein motif:HMM:NF024884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HEY5165882.1"
                     /translation="MEPTLHRPSIDARLGWSGFAIAVLGPVAVASALLPLRGDLQPSN
                     VALVLVLAVLVAAVSFDYLFTRPYYSFSINNGDDVQTVVRLLAVGLAVGEIVARSRQS
                     RAIAQRRSREAEHLRHVAELSAGGESPGRLVTVVQNELTQIRDLKSCRFERPPFPTSL
                     PQLRHRGIRLETEPPDQIGLHDQLELPVRGGGRVLGRFVLTMPTDGTGSACPLKAVPL
                     PSR"
BASE COUNT          348 a          650 c          681 g          340 t
ORIGIN      
        1 cacaaccgaa cgtctgacgg tcgactgtcg gctggggcca ccgccaacag gcctcccacg
       61 ccaagaacca cctcgcggac ggcaaaggtt cgtgtcatca gggtgtccgt agcagcagac
      121 cctctagtaa gccggccagg cgcaattacg aacgctacgc cgatcgccac cctcgtggcg
      181 gcgaacgcgc cgacgacagc cctgccgctc gagttgaaca cggagcgaag gtagcccgtc
      241 cgaccggggg cgtccccgca gacgcgtctc attcccccca atgggtggat gagcgacggt
      301 cagggacggt cacgacctca caaattcgat cgagaagacc gacgatcacc ggcgcgacaa
      361 atcgcagcgt cgacgagctt tggacaggcg cctcgcagtc ggatcgccct gtaagggcga
      421 cgtcattcgt atgcacgtgt cggttccctg gctgttgacc cacgagacgt cttggcgcga
      481 tcacgttgac ggcgacgtct actcctcagg tgaccagctg gtgcagttcc caggtcgtcg
      541 ggggccccca tatcggcgtg gccccgtggc gctccatggc cggccggagt gtcgtgtcca
      601 tgaagtgttt ggcctgttcc gggctgtccc agacgctgac gtcccgccaa gtcgagccgt
      661 ccggtcccac tcccgcgatg tgcgcccgac agccctcagg caacgaattg ctcagcccca
      721 gctcggcggc gacgcggtcg tattgcttgc tggtcatgtt cggcgtttcg gacagcacga
      781 cgatcgacat ggttgctccc ttttcggtgg tgggcggtca tgatctcggt catctaggtt
      841 cgtcggttgg ctgttcgccc tggggttcag gccggggcgg ggtgatgcag cggcgtcttc
      901 agtcgagcag ggcaagccac atggcgctga agtgtgcgaa tcgcgactca agatttgcct
      961 gaccatccca cggcaagagc gacttgatcc gcacacaccc tttggtccga gttacgaccc
     1021 gcggagccat cgcacggcac caccaagacg ccggttccgc ctcacgggcg tacgcaaccc
     1081 gcgtcaggtg gggtcaccgc cggactcggc gagcgcggcg ccgcgctgat ccgctaacgc
     1141 gacggcaaag gaacggcttt cagcgggcag gcggagcccg tcccgtccgt gggcatggtc
     1201 agcacgaacc gcccgaggac gcgcccgccg ccgcgaacgg gtagctcgag ctgatcgtgc
     1261 agaccaatct ggtcgggagg ttctgtttcg aggcggatgc cacggtggcg taactggggc
     1321 agcgatgtcg ggaacggtgg tcgttcgaac cggcagctct tgaggtcgcg gatctgggtg
     1381 agttcgtttt gcacgaccgt cacgaggcga cccggggatt cgccgccggc gctgagctct
     1441 gcgacgtgtc gcaggtgctc cgcttctcgg cttcgtcgct gtgcgatggc tcgactctgc
     1501 cggcttcgag ccacgatctc accgacggcg agcccgacgg cgagcagccg gacgaccgtc
     1561 tggacgtcgt cgccgttgtt gatcgagaac gagtagtagg gacgggtgaa caagtagtcg
     1621 aatgacaccg cggcgacgag gaccgcgagg accaggacca gcgcgacgtt cgagggttga
     1681 aggtcccccc gaagcggaag cagtgcggac gcgacggcga cgggaccgag cacggcgatg
     1741 gcgaaccctg accacccgag tcgagcgtcg atggacggac ggtgcagcgt tggttccata
     1801 gaatggtgga tcctcgatct cgggcacgtc gccgtgacag atcccgccgt tcaccgatcg
     1861 taccgttcga ctccgagcgc acttcggtgc cccgaccggc cggtcgggcc gagggggcgc
     1921 ccggtatcag gcaccgacag gacagcccgg accggctcac cgaatcgggt gtcgcctgcc
     1981 gcttggacgt cgtcgccgtt gttgatcgag aacgagtag
//
LOCUS       DATCCH010000417         2150 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_447477, whole genome shotgun sequence.
ACCESSION   DATCCH010000417 DATCCH010000000
VERSION     DATCCH010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447477"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1335
                     /locus_tag="VIJ44_08030"
     CDS             <1..1335
                     /locus_tag="VIJ44_08030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEY5165883.1"
                     /translation="DQARGWLADHANLESLGIPTGTTRRIQHPTLARMEALATLLGSP
                     QLEYPVLHLTGTNGKTSATRLATALLVESGLSVGAYTSPHLERVNERISWNEHEIDDE
                     TLDELLTLVATIEPHLPEAPSYFEILTGVAYRWFADVAVDAAVIEVGVGGRWDATNVA
                     AGRVAAVTNVTVDHVEYLGRTPLDIARDKAGIVKPGGTLVLGETDPTLQAPFLERGAS
                     RVLERGRDFGVTANRVAHGGRLISLFTPEARYDDVFLSLHGAHQADNAAVAVAVVEAL
                     LDRPLDADLVTAVLGQARSPGRLEVVGHAPLLLLDGAHNVAGAHALRDALAEAFPASA
                     RTLVVGLLREKDPPEMLEALGVADAAHVIVCEAPSPRAHDPEVVAAAAHRLGPPADRV
                     EIVSDVAAAVDRALTVTPADGQVVVSGSLYTVGAARAARGHPSGGETAAGPG"
     gene            1346..1759
                     /gene="ndk"
                     /locus_tag="VIJ44_08035"
     CDS             1346..1759
                     /gene="ndk"
                     /locus_tag="VIJ44_08035"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012851898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEY5165884.1"
                     /translation="MSSRTLFLCKPDAVERGLVGEIVHRVERKGIRIVAMDLRRIDAD
                     LAGRHYEEHRDKPFFADLVTFITSGPLVAMVLEGGGDTWAVVRRLMGATNPREADPGT
                     VRGDLALETGQNLVHGSDGPESAAREIALFFPGLA"
     gene            1950..>2150
                     /locus_tag="VIJ44_08040"
     CDS             1950..>2150
                     /locus_tag="VIJ44_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019983803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEY5165885.1"
                     /translation="MAVDLGTANTVVYARGQGIVLNEPSIVAINARDGRILAVGAQAK
                     RMLGRTPGHIEAIRPLKDGVIAD"
BASE COUNT          304 a          825 c          708 g          313 t
ORIGIN      
        1 gaccaagctc gggggtggct tgcggaccac gcgaacctgg agtcgctcgg catcccgacc
       61 ggcaccaccc gacggatcca gcatccgacg ctcgcccgga tggaggccct cgccacgctc
      121 ctcggatcac cgcagctcga gtatccggtg ctgcacctca cgggcacgaa tggcaagacg
      181 tccgccaccc ggctcgccac cgcgctcctg gtcgagagcg gactgtccgt cggtgcttac
      241 acgagcccgc acctcgagcg ggtgaacgag cggatctcct ggaacgagca cgagatcgac
      301 gacgagaccc tcgacgagct gctgaccctc gtcgcgacga tcgagcctca tctccccgag
      361 gcgccgagct acttcgagat cctcaccggc gtcgcctacc gctggttcgc ggacgtcgcc
      421 gtcgacgccg cggtcatcga ggtcggtgtc gggggccgtt gggacgccac caacgtcgcc
      481 gccggacgcg tcgccgcggt cacgaacgtc accgtcgacc acgtcgagta cctcggacgc
      541 acccccctcg acatcgcccg agacaaggcc gggatcgtca agcccggcgg caccctcgtc
      601 ctcggcgaga ccgacccgac cctccaagcc ccgtttctcg agcggggcgc gtcccgggtc
      661 ctcgagcgag gacgcgactt cggggtcacc gccaaccgtg tcgcccacgg cggccgtctc
      721 atcagcctct tcactccaga agcccggtac gacgacgtgt tcctctcgct ccacggggcg
      781 caccaggccg acaacgctgc ggtcgccgtg gcggtggtcg aggcgctcct cgatcgaccc
      841 ctcgacgccg atcttgtcac cgctgtgctg ggccaggccc gctcccccgg acggctcgaa
      901 gtcgtcgggc acgccccgct gctcctcctc gatggtgccc acaacgtcgc cggggcccac
      961 gcgctgcgag acgcgctcgc ggaggcgttt cccgcgtcgg cccgaaccct cgtggtcggc
     1021 ctcctgcgcg agaaggaccc gcccgagatg ctcgaggcct tgggtgtcgc cgacgccgcc
     1081 cacgtcatcg tctgcgaggc gccgagcccg cgggcccacg accccgaggt ggtcgccgcc
     1141 gcggcccacc ggctcggccc ccccgccgac cgggtcgaga tcgtctccga cgtcgccgcc
     1201 gcggtcgacc gcgcgctgac cgtcaccccc gccgacgggc aggtcgtcgt gagcgggtcg
     1261 ctgtacaccg tcggagcggc ccgcgccgcc cggggccacc cttccggcgg cgagaccgcg
     1321 gctggtcccg gatagggtcc cggcggtgtc gtcccggacc ttgttcctct gcaagcctga
     1381 tgcggtcgag cggggcctgg tcggcgagat cgtccatcgc gtcgagcgca aggggatccg
     1441 catcgtcgcg atggacctcc ggcgcatcga cgccgacctg gctggacgtc actacgagga
     1501 gcatcgcgac aagcccttct tcgccgactt ggtgacgttc atcaccagcg gcccgctggt
     1561 ggcgatggtc ctcgaggggg gaggcgacac ctgggccgtg gtccggcggc tcatgggggc
     1621 gaccaaccct cgcgaggccg atccggggac cgtccgcggc gacctcgcgc tcgagacggg
     1681 ccagaacctg gtccacggct ccgacggtcc cgagtccgca gcacgcgaga tcgccctctt
     1741 ctttccgggg ctggcctgaa attccgacgt cgggggcgtc cggctggttg aatcagtgca
     1801 gcgccgccgt ccggtggtgc acccgaggct gtggaggacg gctggaccgc cctcctcgga
     1861 ggtcctagcc tgtgcccact cccggacctc gtcgacgcac cggacggtct gtgaacgccc
     1921 cttggtattc caacgtgatt ggccgcgata tggcggtcga cctcggcacg gccaacaccg
     1981 ttgtctacgc gcgcggccag ggaatcgtcc tcaacgagcc gtcgatcgtc gccatcaacg
     2041 cccgtgacgg ccgcatcctc gccgtcggtg cccaagccaa gcgcatgctg ggccgcaccc
     2101 cgggccatat cgaggccatc cggcccctca aagacggggt catcgccgac
//
LOCUS       DATCCH010000418         2231 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_447523, whole genome shotgun sequence.
ACCESSION   DATCCH010000418 DATCCH010000000
VERSION     DATCCH010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447523"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="VIJ44_08045"
     CDS             complement(<1..232)
                     /locus_tag="VIJ44_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012015020.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HEY5165886.1"
                     /translation="MQRRYRVIIAKPGLDGHDRGAKVIARALRDAGFEVIYTGLFQTP
                     EQVAETCLQEDADAVGLSVLSGAHLSLFSRVLA"
     gene            335..796
                     /locus_tag="VIJ44_08050"
     CDS             335..796
                     /locus_tag="VIJ44_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165887.1"
                     /translation="MPPASSDRRLLLVTASVVVLAALLVGALLFFATGGAQGTPSPKP
                     VYIGLESDLRATLDQGPRLYFAHPFGGTGFWLDTEGAHLVALVARLPGSPSCTVAWKN
                     LSRGYYDCHGRKLSARQLDRYAVQFPKVGPQQGGVIVDFRHVDPAPQGPPR"
     gene            complement(804..>2231)
                     /locus_tag="VIJ44_08055"
     CDS             complement(804..>2231)
                     /locus_tag="VIJ44_08055"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEY5165888.1"
                     /translation="ANAVIANNAARRPKHLWTEQIGGELLTQYQAEDEHDEAIFCAHE
                     VMRLTDTEGYRFRDLAVFYRTNAQSRVIEEMFVRAGIPYRVVGGVRFYDRREIKDTLS
                     YLRAIVNPDDEVSWKRIVNVPKRGVGDTSVAKVDTHARASGLTFRDALREAAVAGVSG
                     RALGGIQDLLDLVSLFEQAAEKGVARTIESILEHSGYLAELESERSIEAQGRIENLQE
                     LIGVGREFDTALDAGDLTGLPAIAGVGSPGESGGSTDTPVVAPTGLDRIQAFLEAVSL
                     VTDLDTAEGEGDDHSAVTLMTLHAAKGLEYPVVFIAGLEDGVFPHLRSLGDPDSLEEE
                     RRLCYVGITRARERLYLCHAWRRTLYGATDYYSPSRFLAEIPEELVHRIGEERTRGHG
                     VSAHREAVVDAALRGATPTGGGASGAQHLGIKIGDDVVHDTFGEGVVIDLEGRGDSIE
                     AVVQFRDLGEKRLLLAWAPLRRAGT"
BASE COUNT          313 a          762 c          782 g          374 t
ORIGIN      
        1 gggccaggac ccgcgagaac aggctgaggt gggcgccgga cagcaccgag agcccgaccg
       61 cgtcggcgtc ctcttggagg caggtctccg ccacctgctc cggtgtctgg aacagcccgg
      121 tgtagatcac ctcgaagccg gcgtcgcgca gcgcgcgagc gatcaccttg gcgccacggt
      181 cgtggccgtc gagacccggc ttggcgatga tgacgcggta gcgccgctgc atgggctggc
      241 acctcggtga tcccaccggc ggccgccggc gggtgggcgc cagcgtatgc agccccgact
      301 ggaccgggcc acccccgccg ggtaccaaag ggtcgtgccc cctgcctcct ctgaccggcg
      361 cctcctgctc gtcaccgcct cggtcgtggt gctcgccgcg ctcctggtcg gagccttgtt
      421 gttcttcgcg accggtgggg cgcaaggcac cccgagcccc aagccggtct acatcggctt
      481 ggagagcgac ctccgagcga cgctggacca aggcccgcgg ctgtatttcg cgcacccctt
      541 cggtggcacc gggttctggc tcgacaccga gggtgcgcat ctggtggcgc tggtggctcg
      601 gctgcctggc agcccgtcgt gcaccgtggc ctggaagaac ctgagccgcg gctattacga
      661 ctgtcacggc cgcaagctgt ccgcccgaca gctggaccgc tacgcggtgc agttccccaa
      721 ggtcggtcct cagcagggcg gcgtcatcgt ggacttccgc cacgtcgacc cggcgcctca
      781 gggtccaccg cgctgagatc gggctaggtg ccggcgcgtc gcaggggcgc ccaggcgagc
      841 aggagccgtt tctcgcccag atcgcggaac tgcacgaccg cttcgatcga gtcgccgcga
      901 ccctcgaggt cgatgaccac gccttccccg aaggtgtcgt gtaccacgtc gtcgccgatc
      961 ttgatcccga gatgctgcgc gccgctcgcg ccaccgccgg tgggtgtggc cccgcgcagc
     1021 gcggcgtcga cgacggcttc gcggtgcgcg ctgaccccgt ggccgcgggt gcgctcctcg
     1081 ccgattcggt gaacgagctc ctcggggatc tcggcgagga agcggctcgg ggagtagtag
     1141 tcggtcgccc cgtagagcgt gcgccgccag gcgtggcaca ggtagagacg ctctcgggcc
     1201 cgggtgatgc ccacgtagca gagccgacgt tcctcttcga gcgagtcggg gtcaccgagg
     1261 ctgcgcaggt gcgggaacac gccgtcctcg agaccggcga tgaacacgac cgggtactcg
     1321 aggcccttgg cggcgtgcag ggtcatgagg gtgaccgcgc tgtggtcgtc gccttcacct
     1381 tcggcagtgt cgaggtcggt gacgagcgag acggcctcga ggaacgcctg gatccggtcg
     1441 aggccggttg gcgcgacgac cggggtgtcc gtcgagccac cgctctcgcc ggggctcccg
     1501 actccggcga tcgcgggtag gccggtgagg tcgccggcgt cgagagcggt gtcgaattcg
     1561 cggccgacgc cgatgagctc ttggaggttc tcaatccgtc cctgcgcctc gatcgagcgt
     1621 tcggactcga gttccgcgag gtatcccgaa tgctcgagga tcgattcgat cgtgcgggcg
     1681 acgcccttct cggcagcctg ttcgaacagg ctgacgaggt ccaagaggtc ctgaatgcct
     1741 cccagggcgc ggccggacac tccggcgacc gccgcttcgc ggagcgcgtc acgaaaggtc
     1801 aaccccgatg ctcgagcgtg ggtgtcgacc ttcgccacgg acgtgtcgcc gaccccgcgt
     1861 tttgggacgt tgacgatccg tttccacgag acctcgtcgt cggggttcac gatcgcccgc
     1921 aggtacgaga gcgtgtcctt gatctctcga cggtcataga aacgcacacc cccgacgacc
     1981 cgatacggaa tcccggctcg gacgaacatc tcctcgatga cgcggctctg cgcgttcgtt
     2041 cgatagaaga ccgccaggtc ccggaaccgg tacccctcgg tgtcggtgag ccgcatcacc
     2101 tcgtgggcgc agaagatggc ttcgtcgtgc tcgtcctccg cctggtactg ggtgaggagc
     2161 tcgccgccga tctgttcggt ccagagatgt ttgggccgcc gggcggcgtt gttggcgatc
     2221 accgcgttgg c
//
LOCUS       DATCCH010000419         5263 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_150846, whole genome shotgun sequence.
ACCESSION   DATCCH010000419 DATCCH010000000
VERSION     DATCCH010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5263
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150846"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1173)
                     /gene="glnA"
                     /locus_tag="VIJ44_08060"
     CDS             complement(<1..1173)
                     /gene="glnA"
                     /locus_tag="VIJ44_08060"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEY5165889.1"
                     /translation="MESRTPKEVLDLAKSVDAAFVDLRFCDLPGIMQHFSMPIHELTE
                     DGFDDGYGFDGSSIRGFQEIQESDMLLIPDPDTAVVDPFREHVTINLNCFVRDPVTGE
                     SYSRDPRYVAKKAEEYLKGTGLADTVYFGPEAEFYIFDQVRFDQNQYSGYYFLDSVEG
                     VWGSGREFELDGEPNLGYKPRYKEGYFPVPPMDSYQDLRSEMVRVMEEVGIEIEVQHH
                     EVGTAGQAEIDMRFDTMLTMADKLMLYKYVVKNVARARGKSATFMPKPIFMDNGSGMH
                     VHQSLWKGGEPLFFDEKGYGGISDMARWYIGGLLKHAATIIAFSNPTTNSFKRLVPGY
                     EAPVNLVYSQRNRSACVRIPITGTNPKAKRIEFRVPDPSANPYLAFPAMLMAGLDGI"
     gene            1364..2686
                     /locus_tag="VIJ44_08065"
     CDS             1364..2686
                     /locus_tag="VIJ44_08065"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006542200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEY5165890.1"
                     /translation="MQHQLDYVLRTVEERGVRFVRLWFTDVLGFLKSFEITPAELETA
                     LEEGMAFDGSAIEGYSRVQESDMLLIPDANTFEIVPWHGDEAPTALIFCDVHRISNEP
                     FEGDPRFVLKRNMERAREKGFTFYVGPEMEYFYFASEGSPEPLDHAGFFDLTTERVSN
                     LRKQTIVTLEAMGIPVEYSHHELGPSQHEIDLRYTDALTMADNVISFRHVVKKIADDV
                     GVYATFMPKPISGDFGSGMHTHLSLFEGDVNAFHDASDALGLSKVGKCFIAGLLHHAR
                     EITAVTNQWVNSYKRLSVRYEAPVYVCWARNNRSALVRVPVTKKGKESSTRIEFRSPD
                     PACNPYLAFSVMLAAGLKGIDEGYELPAEATNNIFELTPEERAAEGIASLPESLGEAI
                     DVMERSEFVAETLGEHIFEHFVRNKRAEWDDYRSQVTPYELDRYLGIL"
     gene            2695..3381
                     /locus_tag="VIJ44_08070"
     CDS             2695..3381
                     /locus_tag="VIJ44_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009078027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5165891.1"
                     /translation="MEPLLIFPDPPPALLAQTLDLAGYRWKAVANVAVAAQHEPRDGW
                     SGAVVVCDEDPEGAFGMCRALRKRDAGLDPVLLLVSGAQLGELELREDLFDDFCLSPF
                     HQKELEARLRHLFWRSGLGTRPEIVEYGDLILNLETYQAALGDRPLDLTYMEYELLKF
                     FATHPGKVFTREQLLSRVWGYEYYGGARTVDVHVRRLRAKLGEEHANWIQTVRSVGYR
                     FGQGRALPRS"
     gene            complement(3416..3565)
                     /locus_tag="VIJ44_08075"
     CDS             complement(3416..3565)
                     /locus_tag="VIJ44_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165892.1"
                     /translation="MAGALVFVVAMVVVVPVGVMVTGALWSAVFGWLEPADIEPQPGS
                     SSGTS"
     gene            complement(3601..>5263)
                     /locus_tag="VIJ44_08080"
     CDS             complement(3601..>5263)
                     /locus_tag="VIJ44_08080"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY5165893.1"
                     /translation="DAAPGESLLSVLRERLGLRSAKDGCAPQGQCGCCTVLVDGAPRV
                     ACVTPATRVDGRHVTTIEGLDPQHRDELASAFLATGASQCGFCTPGILMRASALLDRG
                     AIDPAGIERALAAHLCRCTGWRTVIEAIDAAPTARTSRRLDDAARRAALEGGVPQRVG
                     AAVPLGSGGFADDVAPDGALVAVPLPVGSDAAAVDAVGMCWVVAETLHDARSRAGKVQ
                     GRRSTRDDDGPLPRPSRPDGGVRLTTSWVEPAYLEPDASWCEPGGTPASPLANGGAFG
                     AKTDSLAPAAARALADRLGSTVRVVYSREDVVRLGAKRPPIAATAHLDGRTLQIAGEY
                     VGSPMRWDPAPPVNDLAIEERWVAAARPGPPTSPALRAFPLAEQTVLREGAVTESGLD
                     RGALCVDERVAATRLLTVAAAPGGALGGAAVELDPVTGALARVEVRVGAGDPLDAVVL
                     RSYCVGAAHMALGWVLTEAVAVDAVSGDVLDLTIRSFGILRARSTPPIDVTVLDDPGA
                     PRPRAGDAVFAAVAAAAWEAITAFEGTRPETFPARSTAAARAART"
BASE COUNT          841 a         1785 c         1832 g          805 t
ORIGIN      
        1 gatgccgtcc aggccggcca tcagcatcgc cgggaacgcc aggtacgggt tggccgacgg
       61 gtccggcacg cggaactcga tccgcttggc cttgggattg gtgccggtga tcgggatccg
      121 cacgcacgcc gaacggttcc gctgcgagta caccaggttg accggagctt cgtagccggg
      181 cacgagccgc ttgaacgagt ttgtggtcgg gttcgagaac gcgatgatcg tcgccgcgtg
      241 cttcaaaagc ccgccgatgt accagcgggc catgtccgag atcccgccgt agcccttctc
      301 gtcgaagaac agcggctcgc cgcctttcca cagcgactgg tggacatgca tccccgagcc
      361 gttgtccatg aagatcggct tcggcatgaa ggttgctgac ttgccacgcg cccgagccac
      421 gttcttcacg acgtacttgt agagcatcag cttgtcggcc atggtcagca tggtgtcgaa
      481 gcgcatgtcg atctcggcct ggccggcggt gcccacctca tggtgctgca cctcgatctc
      541 gatgccgacc tcttccatga cccgcaccat ctcggaccgg aggtcttggt acgagtccat
      601 cggcgggacc gggaagtagc cctctttgta gcgaggcttg tacccgaggt tgggttcgcc
      661 gtcgagctcg aactcgcgcc cggagcccca cacgccctcg accgaatcca agaagtagta
      721 gccggagtat tggttctgat cgaagcgaac ctggtcgaag atgtagaact ccgcctcggg
      781 cccgaagtag accgtgtcgg cgagcccggt gcctttcagg tactcctcgg ccttcttggc
      841 gacgtagcgc gggtcacggc tgtacgactc gccggtgacc gggtcgcgta cgaagcagtt
      901 gaggttgatc gtgacgtgct cgcggaacgg gtccaccacc gcggtgtccg gatccgggat
      961 caggagcatg tcggactcct ggatctcctg gaagccgcgg atcgacgaac cgtcgaagcc
     1021 gtagccgtcg tcgaagccgt cttcggtcag ctcgtgaatc ggcatcgaga agtgctgcat
     1081 gatgccgggc agatcgcaga agcgcaggtc gacgaaggca gcgtcgacgc tcttggcgag
     1141 gtccaggacc tccttcgggg tacggctctc cactgtgttc ctccgtgacg gtcggcgata
     1201 accccacccg gtcgttccgt gaccgcggct cgccgatgtt ggggcgatgc tggcacgtca
     1261 aatggcacgc cgcctgatcc tgactgtgaa cgccgtatga acaggcccgg gaagcggtgc
     1321 gccgcccgcc ggcccggagg ggatcgcctg ccaaactgga gacatgcagc atcagctcga
     1381 ttacgtcctt cggaccgtgg aggagcgcgg ggtgcgattc gtgcggttgt ggttcaccga
     1441 cgtgctcggg ttcctcaaga gtttcgagat caccccggcc gagctcgaga ccgccctcga
     1501 ggagggcatg gccttcgacg ggtccgcgat cgagggctac agccgggtgc aggagtcgga
     1561 catgctcctg atcccggatg ccaacacgtt cgagatcgtc ccctggcacg gtgacgaggc
     1621 cccgacggca ctgatcttct gtgacgtcca tcgcatctcg aacgagccct tcgagggcga
     1681 cccgcggttc gtcctgaagc gcaacatgga gcgggcccgg gagaagggat tcacgttcta
     1741 cgtcgggccc gagatggagt acttctactt cgcgtcggaa ggctcacccg agccgctcga
     1801 ccacgccggg ttcttcgacc tgaccaccga acgggtgtcg aaccttcgca agcagaccat
     1861 cgtcactctc gaggcgatgg ggatcccggt cgagtacagc caccacgagc tcggcccgag
     1921 ccagcacgag atcgacctgc ggtacaccga cgcgttgacg atggccgaca acgtcatctc
     1981 gttccgccac gtggtgaaga agatcgccga cgacgtcggc gtgtacgcca cgttcatgcc
     2041 gaagccgatc tcgggcgact tcggctccgg gatgcacacc cacctgtcgc tcttcgaggg
     2101 cgacgtgaac gccttccacg acgcgagcga cgcccttggt ctctccaagg tcgggaagtg
     2161 tttcatcgcc gggctgctcc accacgcccg cgagatcacc gcggtcacga accagtgggt
     2221 caactcatac aagcggctca gcgtccggta cgaggcgccg gtctatgtgt gctgggcccg
     2281 gaacaaccgg tcggcgctgg tgcgggtccc ggtcaccaag aagggcaagg agagctcgac
     2341 gcgcatcgag ttccgatccc ccgatccggc ctgcaacccg tatctcgcgt tctcggtgat
     2401 gttggccgcc ggcctcaaag gcatcgacga gggctacgag ctccccgccg aggcgacgaa
     2461 caacatcttc gagctcaccc cggaggagcg ggccgccgag ggaatcgctt cgctgcccga
     2521 atcgttgggc gaggcgatcg acgtgatgga gcggtccgag ttcgtcgccg agacgctcgg
     2581 cgagcacatc ttcgagcatt tcgtgcgcaa caagcgtgcc gagtgggacg attaccggtc
     2641 ccaggtgacc ccctacgagc tcgaccggta tctcggcatc ttgtgatgga gatcatggag
     2701 ccgcttctca ttttccccga cccgccgccg gcgctgctgg cccagaccct cgacctggcc
     2761 gggtaccgct ggaaggcggt cgcgaacgtc gccgtcgcgg ctcagcatga gccgcgcgac
     2821 gggtggtccg gagcggtcgt ggtgtgcgac gaggacccgg agggggcgtt cggcatgtgc
     2881 cgggcgttgc ggaaacgcga cgccggcctg gacccggtgc tgctcctcgt ctccggcgcg
     2941 cagctcggcg agctggagtt gcgcgaggac ctcttcgatg acttctgcct ctcgccgttc
     3001 caccaaaagg agctcgaggc tcgcctccgc cacctgttct ggcgcagcgg gctcggcacc
     3061 cgacccgaga tcgtcgaata cggcgacttg atcctgaacc tcgagacgta tcaggcggcg
     3121 ctcggcgatc gacccctcga cctgacctac atggagtacg agctgctgaa gttcttcgcc
     3181 acccacccgg ggaaggtctt cacgagggag cagctgctct cccgggtgtg gggctacgag
     3241 tactacgggg gggcccgcac cgtcgacgtc cacgtgcgtc gcctgcgcgc caagctgggc
     3301 gaagagcacg cgaactggat ccagaccgtg cgatcggtcg ggtaccgatt cggtcagggc
     3361 cgggcgctgc cgcgcagctg atcgccgaac cgcggcccgg tatcggccgg ggtcgctacg
     3421 acgtcccgct actcgagccg ggttggggct cgatgtcggc cggctccagc cacccgaaca
     3481 ccgcgctcca gagggcgccg gtcaccatga cgccgaccgg caccaccaca accatcgcca
     3541 cgacgaacac cagcgcaccg gccacgaacg ggacgctact cggcgatccc gccgccctgg
     3601 tcaggtgcgg gcggcacggg cggcggcggt cgagcgggcc gggaacgtct cgggtctcgt
     3661 cccctcgaac gcggtgatcg cctcccaggc cgcggcggcg accgcggcga acaccgcgtc
     3721 cccggcccgg ggtcgggggg cgccggggtc gtcgagcacg gtgacgtcga tcggaggggt
     3781 cgatcgggcc cgcaagatcc cgaacgatcg gatggtgagg tcgaggacgt cgccactcac
     3841 cgcgtccacg gccacggctt cggtcaggac ccagccgagc gccatgtgcg cggcgccgac
     3901 gcagtacgag cggagcacga ccgcgtcgag cggatcaccg gcaccgacgc ggacctcgac
     3961 tcgggccagc gcaccggtca cagggtcgag ctccaccgcg gcgccgccga gcgccccgcc
     4021 cggcgcagcg gccacggtga gaagccgcgt cgccgcgacc cgttcgtcga cgcagagcgc
     4081 gccgcgatcg agtcccgatt cggtgacggc gccctctcgt aacacggtct gctcggccag
     4141 cgggaaggct cgcagcgcgg gcgaggtcgg cggccccggc cgggcggcgg ccacccagcg
     4201 ttcctcgatc gccaggtcgt tgaccggcgg tgcgggatcc cagcgcatcg gcgaaccgac
     4261 gtactccccg gcgatctgca gggttcggcc gtcgaggtgc gcggtggcag cgatcggggg
     4321 ccgtttggcg ccgagccgga cgacgtcctc ccgcgagtac acgacgcgga ccgtggatcc
     4381 gagccggtcg gcaagcgccc gggcggccgc cggggccaag gagtcggtct tggcgccgaa
     4441 cgcacccccg ttggcgagcg gcgacgcggg cgtcccaccc ggctcacacc acgacgcgtc
     4501 cggctcgaga taggcgggct cgacccagct ggtggtgagc ctcactccac cgtcaggccg
     4561 cgacgggcgc ggaagcgggc cgtcgtcgtc gcgcgtcgag cggcgtccct gaaccttgcc
     4621 ggcacggctg cgggcatcgt ggagggtctc ggcgacgacc cagcacatcc cgacggcgtc
     4681 gaccgcggcc gcgtcggagc cgacgggaag cggaacggcg acgagggcgc cgtcgggggc
     4741 gacgtcgtcg gcgaagccgc cgctgcccag cggcacagcg gcaccgaccc gttgggggac
     4801 gccgccttcg agggcggctc gccgcgccgc gtcgtcgagc cggcgagacg tccgggccgt
     4861 tggggcggca tcaatcgcct cgatgaccgt ccgccagccg gtgcagcggc acaggtgcgc
     4921 ggcgagggcc cgctcgatac cggccgggtc gatggcgccc cggtcgagga gggccgaggc
     4981 tcgcatcagg attcccggcg tgcagaatcc gcactgggac gcgccggtcg cgaggaaagc
     5041 tgaggcgagc tcgtctcggt gttggggatc gagcccttcg atggtcgtga cgtggcgccc
     5101 gtcgacgcgc gtggcgggcg tgacgcacgc gacccgaggc gcgccgtcga cgaggacggt
     5161 gcagcacccg cattgacctt ggggggcgca tccgtccttc gcggagcgga gcccgagccg
     5221 ctcgcgcagc accgacaaga gcgactcgcc gggagcggcg tcg
//
LOCUS       DATCCH010000420         2621 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_26475, whole genome shotgun sequence.
ACCESSION   DATCCH010000420 DATCCH010000000
VERSION     DATCCH010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26475"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="VIJ44_08085"
     CDS             complement(<1..577)
                     /locus_tag="VIJ44_08085"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY5165894.1"
                     /translation="MRTRLSVAVVGIILAALLAACRPLPPPPPPPPPPPPPGPLSLGW
                     SDPGVTGPIAADAPENLVWATGGGQLWGLNAATGAVVAHHALTLDGVQHFPTPNVVSS
                     WVVIESRQQVAGFPTPANPSGVAWTSAVLDGEVQSRPAIVGATVVVATEDDSLYGLSL
                     LDGHTLWGPTNFGTPEPLADIRTLGGVPNCGN"
     gene            774..1358
                     /locus_tag="VIJ44_08090"
     CDS             774..1358
                     /locus_tag="VIJ44_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019354494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY5165895.1"
                     /translation="MADQARFADLAMSYMDALYSAAMRMTRNPADAEDLVQETYLRAY
                     RGFGGFKDGTNLKAWLYKILTNTFINSYRAKKRRPEETDLDDTEDFYLYRRIGGLEAA
                     AAERTPETEVLEAIPEAVVKEALEALPEQFRMAVLLADVEGFSYKEIAEIMDVPIGTV
                     MSRLHRGRKQLQERLWSFAQERGLLPEPATAGGS"
     gene            1404..1640
                     /gene="rsrA"
                     /locus_tag="VIJ44_08095"
     CDS             1404..1640
                     /gene="rsrA"
                     /locus_tag="VIJ44_08095"
                     /inference="COORDINATES: protein motif:HMM:TIGR03988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol system anti-sigma-R factor"
                     /protein_id="HEY5165896.1"
                     /translation="MDCEQAFHRMYHYLDGELTVWRRWVITRHLDRCPPCAHGFDFEI
                     ELRQVIASRCRDTVPPELKLRVANALGLPVEEER"
     gene            1663..1881
                     /locus_tag="VIJ44_08100"
     CDS             1663..1881
                     /locus_tag="VIJ44_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165897.1"
                     /translation="MKTVRLAAVTVGGLLVFASPAGASTPVVAGQMWYFWLAPILTVS
                     AIGFVLSLWSGYIRKVLFPKYRGRKVQD"
     gene            1878..>2621
                     /locus_tag="VIJ44_08105"
     CDS             1878..>2621
                     /locus_tag="VIJ44_08105"
                     /inference="COORDINATES: protein motif:HMM:TIGR03883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEY5165898.1"
                     /translation="MTSAAPIAAERASAPDPVDWALAQRVARRIAGRDLLTDSYLAAS
                     LRDDFAAVTHQAEELVGDYTGLRADGAARAAVLDRSGWVDVNVASMRRLLGPLTDRLG
                     DRMSHSPMAPIGRSVAATELGVLLGWFAQRVLGQYDLLVPDDTDGGALDGDAVYYVGP
                     NVLGLEKRFAFRPRDFRLWVAIHEVTHRAQFCGVPWMRGYFLDLVGRAMGMVDTDPRA
                     LVKSLLRAADEIRQGRNPLDDGGLVALLAT"
BASE COUNT          398 a          859 c          922 g          442 t
ORIGIN      
        1 tgttcccgca attcgggacg cccccgagag tccggatatc agcgagcggt tcgggggtgc
       61 cgaagttggt gggaccccag agggtgtggc cgtcaaggag gctgagcccg taaagcgagt
      121 cgtcctcggt ggccacgaca acggtggccc cgacgatcgc ggggcgcgac tgcacctcgc
      181 cgtcgaggac cgctgacgtc catgccacgc cggacggatt cgccggggtg gggaagccgg
      241 caacctgctg cctgctctcg ataacgaccc agctgctcac gacgttcggt gtcgggaagt
      301 gctgcacgcc atccagcgtg agggcatggt gcgcgacgac cgcgccggtc gcggcgttga
      361 gcccccacag ctgtccgcca cccgttgccc acacgaggtt ctcgggcgcg tcggcggcga
      421 tgggcccagt taccccggga tcgctccatc cgaggctcag cggtccgggt ggtgggggag
      481 gcggcggggg cggcggggga ggcgggagcg ggcggcaggc ggcgaggagc gcagccagga
      541 tgattccgac gacggcgacc gacagtctcg tgcgcatctc gtcctccggg cctgctctgg
      601 cggtttgtcg accaccgagc gcttcaacct taaaggtccc cgcagcgtgc ggtaccgatg
      661 cagacccgga atgggccagt cgagcgggcc tttcggtcgg ctgggggagt gttggtgagt
      721 tgttcgcgat acccgaatgg tttcgtcgca cctcgggcgt atgctccgcg cctatggccg
      781 atcaggcccg tttcgccgac ctcgcgatgt cctacatgga tgcgttgtac tcggctgcca
      841 tgcggatgac ccgcaatccg gcggacgccg aggacctcgt ccaagagacg tatctgcgcg
      901 cctaccgggg cttcggtggg ttcaaggacg gcacgaacct caaggcctgg ctctacaaga
      961 tcctgacgaa caccttcatc aactcgtacc gggccaagaa gcggcgtcct gaggagaccg
     1021 atctcgacga caccgaggac ttctacctct accggcggat cggcggcctc gaagctgccg
     1081 ccgccgagcg gaccccagag acggaggtcc tcgaggcgat cccggaagcg gtcgtgaaag
     1141 aggcgctcga agccctgccg gagcagttcc ggatggcggt gctgctcgcc gacgtcgagg
     1201 gcttctccta taaggagatc gccgagatca tggacgttcc catcgggacc gtcatgagcc
     1261 ggctgcatcg tggaagaaag cagctccaag agcggttgtg gagtttcgcc caggaacgcg
     1321 ggctgcttcc cgaaccggca accgccggtg ggtcctagct tcggactcgg gaacggcaca
     1381 gggggaaatt gaggaccgtc aggatggatt gcgaacaggc gttccatcgc atgtaccact
     1441 atctggacgg tgagctcacc gtctggcggc ggtgggtcat cacccggcac ctcgaccggt
     1501 gcccaccgtg cgcgcacggc ttcgatttcg agatcgagtt acgccaggtc atcgccagtc
     1561 gctgtcgcga cacggtcccc ccggagctga agctgcgagt cgcgaacgcg ctcggcctcc
     1621 cggtcgaaga ggaacggtga tccggcccgt atgctggctg gcgtgaagac ggttcgactt
     1681 gccgcggtga ccgtgggggg actcctggtg ttcgcgtcgc ccgcgggcgc gtcgacgccc
     1741 gtcgtggcgg gccagatgtg gtacttctgg ctcgcaccga tcctcacggt gagcgcaatc
     1801 ggtttcgtgc tgtccctgtg gagtgggtac atcagaaagg tgctgttccc gaagtaccgg
     1861 ggtcgcaagg ttcaggattg acttccgccg cgccgatcgc tgcggaacgg gcctcggcgc
     1921 ccgacccggt cgactgggcg ctcgcacagc gggtggcgcg tcgcatcgcc ggccgggacc
     1981 tgctcaccga ctcgtatctc gcggcgtcgc ttcgcgacga cttcgcggcg gtcacgcacc
     2041 aggccgagga actcgtcggc gactacaccg gcctgcgcgc cgacggcgcg gcgagggcag
     2101 cggtcctgga ccgctccggt tgggttgacg tcaatgtcgc gtcgatgcgc cgcctcctcg
     2161 gtccgctgac cgaccggctc ggtgatcgca tgtcccacag cccgatggcc ccgatcggcc
     2221 gttcggtcgc cgcgaccgag ctcggtgtcc tcctcggctg gttcgcgcag cgggtcctcg
     2281 gccaatacga cttgctggtt cccgacgaca ctgacggggg cgccctcgac ggcgacgccg
     2341 tctattacgt gggcccgaac gtgctcggac tcgagaagcg gttcgcgttt cggcctcggg
     2401 acttccggct ctgggtcgcg atccatgagg tcacccaccg ggcccagttc tgcggcgtcc
     2461 cgtggatgcg gggctacttc ctcgacctgg tgggccgggc gatggggatg gtcgacaccg
     2521 acccgcgggc gttggtcaaa tcgctgctcc gcgccgccga cgagatccgt cagggccgca
     2581 acccgctcga cgacggggga ctcgtcgcgc tgctcgccac c
//
LOCUS       DATCCH010000421         3133 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_448274, whole genome shotgun sequence.
ACCESSION   DATCCH010000421 DATCCH010000000
VERSION     DATCCH010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448274"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="VIJ44_08110"
     CDS             <1..284
                     /locus_tag="VIJ44_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="anion-transporting ATPase"
                     /protein_id="HEY5165899.1"
                     /translation="PEPFTRADEPVFDALAAGPGRDALVAAAGPGTDAALDATRLAVA
                     LRRSRAVHLAHLREAVDVPLLYLPYLFVRDHGLRVTRVVAEHLAAELGR"
     gene            281..1468
                     /locus_tag="VIJ44_08115"
     CDS             281..1468
                     /locus_tag="VIJ44_08115"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HEY5165900.1"
                     /translation="MTSASLEPLLASREVVVFCGSGGVGKTTTAAAAGLAAVTELGGK
                     VLVLTIDPARRLADALGLEGIGNVERRVPDEVLRAAGLEPRGQLFAAMLDTKASWDDL
                     VLRHAPNEETAYQILENRLYQNITARFVQSHDYIAMERLYDLHTTGTYDLIVVDTPPT
                     RNAIDFLEAPSRMADFFGGPLLRWLTMPYRLGGRRGARVLNFASRPFYQLADRVLGSQ
                     FLQDIAEFFLNFQSMYDGFVVRAHAVERLLHDRRTSFSVVTTLEGAPLREAEFFCREL
                     AARGFPLGAIVLNRVLPEFLTSPEGHTAAAVLCDRAESLAGPLAELGVDALDDPDRTA
                     RVLRTIGSTFLDYGVVATRETDLRNELARAPDVVATVPAFADDIHDIAGLARISRALL
                     ASP"
     gene            1479..2966
                     /locus_tag="VIJ44_08120"
     CDS             1479..2966
                     /locus_tag="VIJ44_08120"
                     /inference="COORDINATES: protein motif:HMM:NF023702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY5165901.1"
                     /translation="MAIGLSDLAQANTGLRGAPFRHLQRLVASWPPLADLCFADLIAL
                     APVEGEEGHRFVVLAHVRPVTGQTLYPGELVGTVVQEVERPLVARAWRKHEIVGGDAT
                     VLGSPERARTQCIPLMLEGEPIAIVSRESPTTSGRRQGELERAYLDTFERFARMMTEG
                     SFPFPRDEVQLEEAPRVADGVILLDEQLCIRFTSPNAISSLHRMGIHSYTHGVQLAEI
                     GFDQDAADDAYRLRVPVTEEIERGETSLLIQAIPLLEHGRPTGAMILVRDVTDLRRRD
                     RALMSKDATIREIHHRVKNNLQTIAALLRLQGRRLQSEEAQEAIQESERRIRSIAIVH
                     ETLSREAADVVNFSDIVGPLVRVVEDTVSTPELRLHFDVEGDAGELPGEVVTPLAVVL
                     NELMQNAVDHAFPINEDGAAEGHVRVWLRRGEGELAVDVVDDGVGLPPGFSLDRSRGL
                     GLSIVHALVTSELGGSMEMRDEGGTRVSLRVPVKPAVRVEGLPAR"
     gene            complement(2967..>3133)
                     /locus_tag="VIJ44_08125"
     CDS             complement(2967..>3133)
                     /locus_tag="VIJ44_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEY5165902.1"
                     /translation="IETARRICTACLVNDECLEFALATNQEAGIWGGTTEEERRKLRK
                     VWVARQRAAC"
BASE COUNT          458 a         1124 c         1061 g          490 t
ORIGIN      
        1 tccccgagcc gttcacccgc gccgacgagc cggtcttcga tgcgctcgcg gccgggccgg
       61 gccgcgacgc gctcgtcgcc gcggcgggac cgggcaccga cgccgcgttg gatgccaccc
      121 gcctcgcggt ggcgttgcgc cgctcccggg ccgtgcacct ggcacatctg cgtgaagcgg
      181 tcgacgtgcc gttgctgtac ctcccgtacc tcttcgtccg agaccacggg ttgcgagtca
      241 cccgggtcgt cgccgagcac ctcgcggcgg agctcggccg gtgacgtcgg ccagcctcga
      301 gccgctgctc gcctcccgcg aagtcgtggt gttctgcggg tcgggcggtg tcgggaagac
      361 gaccaccgcc gccgccgcgg gtctcgccgc ggtgaccgag ctcggcggca aggtcctggt
      421 cctcaccatc gacccggctc ggcgcctggc cgacgcgctc ggtctcgagg gcatcggcaa
      481 cgtcgagcgt cgcgtccccg acgaggtgct ccgcgccgcg gggctcgagc cacgggggca
      541 gctgttcgcc gccatgctcg acacgaaggc gtcgtgggac gacctggtgc ttcgccacgc
      601 gcccaacgag gagaccgcgt accagatcct cgagaaccgc ctctatcaga acatcacggc
      661 ccggttcgtg caaagccacg actacatcgc gatggaacgc ctctatgacc tgcacaccac
      721 tggcacctac gacctgatcg tcgtcgacac cccacccacg cggaacgcca tcgacttcct
      781 cgaggccccg agtcgcatgg cggacttctt cggcggcccg ctcttgcgct ggctgacgat
      841 gccgtaccgg ctcgggggcc ggcggggagc gcgggtgctc aactttgcca gccggccctt
      901 ctaccagctc gccgaccggg tgctcggcag ccagttcctc caggacatcg ccgagttctt
      961 cttgaacttc cagagcatgt atgacgggtt cgtggtccgg gcccacgcgg tcgaacggct
     1021 cctccacgac cggcggacct cgttcagcgt cgtcaccacc ctcgaagggg cgccgttgcg
     1081 cgaagccgag ttcttctgca gggagctcgc ggcccgcggc ttcccgctcg gcgcgatcgt
     1141 gttgaaccgg gtcctacccg agttcctgac gtcccccgag gggcacaccg cggccgccgt
     1201 gctgtgcgac cgggccgagt cactcgccgg accgctcgcc gagctgggcg tcgacgcgct
     1261 cgacgacccc gaccgcaccg cgcgagtgct gcgcactatc gggtcgactt ttctcgacta
     1321 tggcgtcgtc gcgacgcgcg aaaccgatct gaggaacgag ctggcgcggg ccccggacgt
     1381 ggtggcgacg gtccccgcgt tcgccgacga cattcacgac atcgcgggac tggcccgcat
     1441 cagccgcgcc ctcctcgcga gtccgtaacc tctccaccat ggctatcggg ctgtccgacc
     1501 tcgcgcaggc gaacaccggt ctgcgcggcg caccgttccg ccacttgcag cgcctcgtcg
     1561 cctcgtggcc gccgctggcc gacctgtgct tcgccgatct catcgcgttg gcgccggtcg
     1621 agggcgaaga gggacaccgg ttcgtcgtac tggcccacgt gcggcccgtg accggccaga
     1681 ccctctaccc cggtgagctg gtcgggaccg tggtccagga ggtcgagcgt cccctcgtcg
     1741 cccgggcgtg gcgcaagcac gagatcgtcg gtggtgacgc gacggtcctc ggctcaccgg
     1801 aacgggcccg cacccagtgc atcccgctca tgctcgaggg cgagccgatc gccatcgtca
     1861 gtcgagaatc gccgaccacg tcgggccggc gccaaggcga gctcgaacgc gcctacctcg
     1921 acaccttcga gcggttcgcc cgcatgatga ccgagggctc gttcccgttc ccacgcgacg
     1981 aggtgcagct cgaggaggcc ccccgggtcg ctgacggcgt gatcctgctc gacgagcagc
     2041 tgtgcatccg gttcacgagc ccgaacgcga tcagctccct gcaccgtatg gggatccact
     2101 cgtacaccca cggggtgcag ctggccgaga tcggattcga ccaagacgcc gccgacgacg
     2161 cctaccggct acgggtgccg gtcaccgagg agatcgagag gggtgagacg tcgctgctga
     2221 tccaggccat cccgctcctc gaacacggac gtccgacggg ggcgatgatc ctggtgcgag
     2281 acgtgaccga tctccggcga cgtgaccgcg cgctgatgtc gaaggacgcc acgattcgcg
     2341 agattcacca ccgggtgaag aacaacctgc agaccatcgc cgcgttgctg cgcctgcaag
     2401 ggcgacggtt gcagtccgag gaggcccaag aggcgatcca ggagtcggag cgtcggatcc
     2461 gctcgatcgc gatcgtccac gagacattgt cccgagaggc cgccgacgtg gtgaacttct
     2521 ccgacattgt cggtcccctc gtccgggtcg tcgaagacac ggtgtcgaca ccggagctgc
     2581 ggctgcactt cgatgtcgaa ggcgacgccg gcgagctccc cggtgaggtc gtcaccccgt
     2641 tggcggtcgt gctcaacgag ctcatgcaga acgccgtcga ccacgcattt cccatcaacg
     2701 aggacggcgc ggccgaaggt cacgtccggg tctggctgcg acgcggggaa ggggagctgg
     2761 cggtggacgt cgtcgacgac ggcgtcggcc tcccgcccgg gttctcgctg gaccggtccc
     2821 gaggattggg gctctccatc gtgcacgccc tggtgaccag cgagctcggc ggctcgatgg
     2881 agatgcgcga cgaaggcggg acgcgtgtca gcctgcgggt gccggtgaaa ccggcggtgc
     2941 gggtcgaagg actcccggcc cgctgatcag caggcagcgc gctggcgggc cacccagacc
     3001 ttgcggagct tgcggcgctc ctcctcggtg gtgccccccc agatgccggc ctcctggttt
     3061 gtcgccaacg cgaactcgag gcactcgtcg ttgacgaggc aggcggtgca gattcgacgg
     3121 gcggtctcga tct
//
LOCUS       DATCCH010000422         2360 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_360037, whole genome shotgun sequence.
ACCESSION   DATCCH010000422 DATCCH010000000
VERSION     DATCCH010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360037"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="VIJ44_08130"
     CDS             <1..562
                     /locus_tag="VIJ44_08130"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEY5165903.1"
                     /translation="RAGRFRSFGDGSAICFPVVALFGERYITVGAGTVIGPYVSLSAG
                     ISPAHELGDRAVVTIGDRCLIGKGSGIVGHDAIEIGDDVYTGHNVYITDANHGYEDPD
                     TPIGRQFVDPRPVRIGAGSWLGHGTIVLPGSSIGRHVVVGAGSVVTGALPDFSVAVGN
                     PARVIRTQTLDAQGLVVGVEDVAGDG"
     gene            complement(517..2223)
                     /locus_tag="VIJ44_08135"
     CDS             complement(517..2223)
                     /locus_tag="VIJ44_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165904.1"
                     /translation="MPERPQRSPGGVRTVAVRYLPVVAVIAVIAVVVAVIGGGGGGGS
                     RPTPAKPGALPLTFDEASARGVNVNWGPTCDTSTGRVAVPFSYAPPCVQPPRGPNGGA
                     TAPGVSADTINVVLYQQQPDVLQQAFFQQTGSDASLAAEARTTQAYVDFFQAHYETYG
                     RKVHLITFRATGSPDDDVAAKADAITVAAQLHAFASFGGPSLTSAYADELAARHVLCL
                     GDCTLAVSTAFVQGRAPYVWPTLPTPEQASQQWAQFVYQQLNGGRAIHAGDPALRRQT
                     RRFGVVHFDDGTGIFDEAYNRFAGLLKKHHTTIAVNLKYQLDLTQAQENARNLIASLK
                     RAHVTSVIMATDPLTPSFLTKEATAQGYFPEWVVLGYAYSDTAVFARTYDQRQWAHAF
                     GVTLLPTRTADSVDEFATILRWQSGHGPEAKTFRVLVQAPLIFFTGVHLAGPNLTAQT
                     FRDGLFRYPADRPLIPTALHTSWGRHGIWPGTDYTWGDDASIIWWDPNISGPDEVSDQ
                     GRGLYRYALGGRRYLPGHLASDVGLYDTAHSATVDTVLPPEARTKNYPSPATSSTPTT
                     KP"
BASE COUNT          348 a          738 c          874 g          400 t
ORIGIN      
        1 gcgggccggc cggttccgtt cgttcggtga tgggagcgcg atctgcttcc ccgtcgtcgc
       61 gctgtttggc gagcggtaca tcacggtggg ggccggcacc gtgatcggcc cgtacgtcag
      121 cctgtcggcg gggatctccc cggcccatga gctcggtgac cgagccgtgg tcacgatcgg
      181 tgatcgatgc ttgatcggga agggcagcgg aatcgtcggg cacgacgcga tcgagatcgg
      241 cgacgacgtc tacaccggtc acaacgtgta tatcaccgac gccaaccacg ggtatgagga
      301 tccggacacg ccgatcgggc gacagttcgt cgacccgcgt ccggtccgca tcggcgcggg
      361 ctcctggctc gggcacggca cgattgtgct ccccggctcg tccatcgggc gtcatgtggt
      421 cgtcggcgcg ggatcagtcg tcacgggcgc actccccgat ttcagcgtgg cggtcgggaa
      481 ccccgcccgg gtgatccgaa cccagacgct cgacgctcag ggtttggtcg tcggtgtgga
      541 ggacgtcgcc ggcgacgggt agttcttcgt gcgggcctcg ggtggcagca cggtgtccac
      601 ggttgccgag tgcgcggtgt cgtacaagcc gacgtcgctg gcgagatgac cgggcaggta
      661 tcggcgtccg ccgagcgcgt agcggtacag cccgcggccc tggtcgctga cctcgtccgg
      721 tccggagatg ttcgggtccc accagatgat cgaggcgtcg tcaccccacg tgtaatcggt
      781 gcccggccag atgccgtgac ggccccagga ggtgtgcagc gcggtcggga tgagtggacg
      841 atcagctgga taccggaaga gcccgtcgcg gaaggtctgc gccgtcaggt tgggtccagc
      901 gaggtgcacg ccggtgaaga agatcagcgg ggcctggacg agcacccgga atgttttcgc
      961 ctccgggccg tggcccgatt gccagcgcag gatggtggcg aactcgtcga ccgagtccgc
     1021 ggtccgggtc ggcagcaggg tgacgccgaa cgcgtgcgcc cactggcgct ggtcgtacgt
     1081 gcgggcgaag accgcggtgt cgctgtaggc gtacccgagc accacccact ccgggaagta
     1141 gccctgcgcc gtcgcctcct tggtgaggaa cgagggcgtc agcgggtcgg tggccatgat
     1201 gacgctcgtc acgtgcgctc gcttcaagct ggcgatgagg ttgcgggcgt tctcctgcgc
     1261 ttgggtgagg tccaactggt atttgaggtt gaccgcgatc gtggtgtggt gcttcttgag
     1321 gagaccagcg aatcggttgt aggcctcgtc gaagatgcct gtcccgtcat cgaaatgcac
     1381 gacaccgaac cgtcgggtct ggcggcgcag ggccgggtcg ccggcgtgaa tcgcgcgacc
     1441 gccgttgagc tgttggtaga cgaactgcgc ccattgttgg gacgcctgct cgggggttgg
     1501 cagcgtgggc cagacgtatg gtgcgcgtcc ttgcacgaat gcggtggaaa cggccagcgt
     1561 gcagtcgccg aggcacagga cgtgacgcgc cgcgagctcg tcggcgtacg ccgacgtgag
     1621 gctgggacca ccgaacgagg caaaggcgtg gagttgggcc gcgacggtga tggcgtccgc
     1681 cttcgcggcg acgtcgtcgt ccggtgagcc cgtcgctcga aaggtgatga ggtggacctt
     1741 gcgcccgtag gtctcgtagt gggcttggaa aaagtcgacg taggcctggg tggtgcgggc
     1801 ttcagcggcg aggctggcgt cgctcccggt ctgctggaag aacgcctgtt ggagcacatc
     1861 gggttgctgt tggtacagca cgacattgat cgtgtcggcc gagacgccag gtgccgtggc
     1921 gccgccgttc gggccccgag gaggctggac acacggcggg gcgtaggaga acgggaccgc
     1981 gactcgtccg gtgctggtgt cgcacgtcgg tccccagttc acgttcacgc cgcgggcgct
     2041 ggcctcgtcg aacgtcaacg gcagcgcacc gggcttggcc ggagtggggc gactaccgcc
     2101 accgcccccg cctccgatga ccgccaccac gaccgcgatg accgcgatga cggcgacgac
     2161 tggcaggtac cggacagcga ccgttcgaac accgccgggt gaacgctggg gcctctcagg
     2221 catcgctcac cggggcatgg tcacccgcgg tccaggtgcg cggtccgatc cccgttcgat
     2281 tcgctgcacc gtgcaccgtc gtaacccggg gcctccttcg gcgggcgagc gtacccccca
     2341 cccccagcga ctccggggtc
//
LOCUS       DATCCH010000423         2220 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_448619, whole genome shotgun sequence.
ACCESSION   DATCCH010000423 DATCCH010000000
VERSION     DATCCH010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448619"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /gene="lepB"
                     /locus_tag="VIJ44_08140"
     CDS             <1..399
                     /gene="lepB"
                     /locus_tag="VIJ44_08140"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEY5165905.1"
                     /translation="GDIVVFKAPPGERTGGINDLVKRVIGLPGETVTERRDSTSSTYN
                     IYVNGRRLHEPYLPTDASSGPEGSSVPPGCGAPADQSPGCVVPPGRVFVLGDNRNQSK
                     DARTFGPITESSIVGRVFVRIWPLSNLSFL"
     gene            complement(430..1131)
                     /locus_tag="VIJ44_08145"
     CDS             complement(430..1131)
                     /locus_tag="VIJ44_08145"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEY5165906.1"
                     /translation="MAPITFAHRGARLLHPENTIPAFRAGLEAGARGLETDAWLSSDG
                     EVVLTHDALVAGPLFGLWKLRVAHTSAARLARRGVPRLTDLFAELGHDYDLSIDVKDP
                     AAAAPIIDMVRRVGDPARTWLCSPSLDQLRTLRDGATDVRLVHSTIRRRLPDSLERHA
                     ADVAAARVDAMNLHYSEWTKGLVALFHRFDVLTFAWDVQEVRHLRGMLDLRIDAVYCD
                     HVERMVSTVSEWSPG"
     gene            1196..1552
                     /locus_tag="VIJ44_08150"
     CDS             1196..1552
                     /locus_tag="VIJ44_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165907.1"
                     /translation="MAIVIKADCPGCGVVRLGARDLTVRVCTDDGSGAYCFRCAECGS
                     AVSHEASPPVCEMLVSAGVKRVDWGWPNDLDDRPGGPRFTHDDLLDFHLLLDHDDEEW
                     SRQLAALTQDADSTST"
     gene            1630..1764
                     /locus_tag="VIJ44_08155"
     CDS             1630..1764
                     /locus_tag="VIJ44_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165908.1"
                     /translation="MSLGPAEILVLAVVVLVFIGPGDSRKRLDTADQRGRTRAVTSPT
                     "
     gene            1842..>2220
                     /locus_tag="VIJ44_08160"
     CDS             1842..>2220
                     /locus_tag="VIJ44_08160"
                     /inference="COORDINATES: protein motif:HMM:NF013097.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEY5165909.1"
                     /translation="MTVIEHLTELRRRILISIFAIVATASLCYWFAPDIIRFFLHYYK
                     DVTHGKKTAFIFTGPLDAFVTRLKVATYSGILLALPVWLWQLWRFITPGLSKREKRYA
                     IPFLASSLVLFAAGATVAMLILPK"
BASE COUNT          334 a          743 c          786 g          357 t
ORIGIN      
        1 ggcgacatcg tcgtgttcaa ggctccgccg ggtgagcgca ccggggggat caacgatctc
       61 gtcaaacggg tgatcggcct cccgggcgag acggtcaccg aacgccgcga cagcacttcg
      121 agcacttaca acatctacgt caacggccgg cgtctccatg agccgtacct gccgacggac
      181 gcgtcgtcag gaccggaggg gtcgtcggtc ccgcctggtt gcggtgcccc cgccgatcag
      241 agcccggggt gtgtcgtccc gcccggacgg gtgttcgtgc tcggcgacaa ccggaaccag
      301 tcgaaggacg cccgaacgtt cgggccgatc acggaatcgt cgatcgtcgg gcgggtcttc
      361 gtccggatct ggcccctcag caacctgtcg ttcctgtagt cgcggctccg gtccggggcc
      421 cggccgggtc tagcccgggc tccattcgct gacggtcgag accatgcgct ccacatggtc
      481 gcagtacaca gcgtcgatcc ggaggtcgag catcccccgc aggtgccgga cctcttggac
      541 gtcccacgcg aaggtgagca cgtcgaaccg gtggaagagc gccacgagac ctttggtcca
      601 ctcggagtag tggaggttca tcgcgtccac tcgggccgcg gcgacgtcag cggcgtggcg
      661 ttcgagcgag tcggggagtc gccgccgtat ggtcgagtgc accagacgca catcggttgc
      721 gccgtcgcgc agggtgcgta gctggtccag gctcggtgag cacagccagg tgcgcgccgg
      781 gtcaccgacg cggcgcacca tgtcgatgat cggcgccgcc gcggccggat ccttgacgtc
      841 gatcgagagg tcgtagtcgt ggccgagctc ggcgaagagg tcggtgagcc gcgggacgcc
      901 acggcgggcg aggcgcgccg cgctggtgtg cgcgacccgc agcttccaga gcccgaacag
      961 cgggccggcg accaaggcgt cgtgggtgag gacgacctct ccgtcggagc tgagccacgc
     1021 gtcggtctcc aggcctcggg ccccagcctc gaggccggcc cggaacgccg ggatcgtgtt
     1081 ctcggggtgg aggagccgag ccccccggtg agcgaaggtg atgggcgcca cagggccgca
     1141 tcctggccga tcagccggcc tcaaggggcc caccgggggt gccgatgcag gagttatggc
     1201 catcgtgatc aaggcggact gccccgggtg tggggtggtg cggctgggag caagggatct
     1261 caccgtccgg gtctgcaccg acgacggttc gggggcgtac tgcttccgat gcgccgagtg
     1321 cggatcggcg gtctcccacg aggccagccc tccggtctgc gagatgctcg tctcggcggg
     1381 ggtcaaacgg gtcgactggg gttggccaaa cgacctcgac gaccggcccg gtgggccccg
     1441 gttcacccac gacgacctcc tcgacttcca tctgctgttg gaccacgacg acgaggagtg
     1501 gagccgacag ctcgccgcgt tgacccagga cgccgactcg acctcgacct gatcgctccc
     1561 ggcccgacaa gggtcgtggc cagggcccga cggtacgatg ccggacggac tcgcgcgaag
     1621 ggagcgtccg tgtcgctcgg cccagccgag atcctggtgc tggcggtggt ggtgctggtg
     1681 ttcatcggcc ccggcgactc ccggaagcgg ctcgacaccg cggaccagcg tggccggacc
     1741 cgtgccgtga cctcgccgac gtgatggtca cgaacgtcgg ggcggacggc acccccgagg
     1801 ggtcggcgta ggacgtgtct ccaaagagtc ccgatgcccg gatgacggtc atcgagcatc
     1861 tcaccgagct gcgtcgacgg atcctcatct cgatcttcgc catcgtcgcc acggcgtcgc
     1921 tctgctactg gttcgcgccc gacatcatcc ggttcttcct ccactactac aaggacgtca
     1981 cgcacgggaa gaagaccgcc ttcatcttca ctgggccgct cgacgcgttc gtcacccggc
     2041 tcaaggtcgc cacctattcg gggatcctgt tggcgctgcc ggtgtggctc tggcagctgt
     2101 ggcggttcat caccccgggg ttgagcaaac gggagaagcg gtacgcgatc ccgttcctcg
     2161 ccagctcgct ggttctcttc gccgcgggcg cgacggtcgc gatgctgatc ctgccaaagg
//
LOCUS       DATCCH010000424         2925 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_448742, whole genome shotgun sequence.
ACCESSION   DATCCH010000424 DATCCH010000000
VERSION     DATCCH010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448742"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /locus_tag="VIJ44_08165"
     CDS             complement(<1..910)
                     /locus_tag="VIJ44_08165"
                     /inference="COORDINATES: protein motif:HMM:NF028011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate cyclase regulatory domain-containing
                     protein"
                     /protein_id="HEY5165910.1"
                     /translation="MDADASTWAERGLYDPDALTAADRLATLRYLAERGATTDDLATA
                     VSDGNLPGLAGELLQRRRPRLTTREATRRAGIPIELGQRINRTAGLGDPAADEATFFE
                     ADIVTFRLFAAAAEMFGVESALQFSRVVGAALSSVADAAMTNFGQGVAPRLDAEQAGE
                     LARTQALEAATLILFDEVPHVLASLFMHHCEAATRRTQASGNLDTSDLTVGFLDLVGS
                     TTLAERLPPAELGALISTFESDATELVSGSDGRVVKTIGDEVMFVTTDAHAACAVALE
                     LAERVECHAVLPHLRGGLAAGPLVRGY"
     gene            1067..1825
                     /locus_tag="VIJ44_08170"
     CDS             1067..1825
                     /locus_tag="VIJ44_08170"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEY5165911.1"
                     /translation="MRHRHRVVLIGAVALATLAAACGGSGGPTLDRKATAAPSTTPAT
                     LPSFISLIARVSVPNVAVYDAPTSPTPSHTFANPWLLNADPKLPVQLVFLVEQRQGPW
                     LQVLLPIRPNGTTGWVRSRDVSVQQNEFRITVELHAHRLVVYNGESVLLSDTVAVGMP
                     ATPTPTGTYFIRVLLKAPDPTTVYGPYAYGLSGHSEALNTFDGGDAELGIHGNNDASV
                     LGTDASHGCIRMSNDKITMLAGVLPLGTPVQITA"
     gene            1874..>2925
                     /locus_tag="VIJ44_08175"
     CDS             1874..>2925
                     /locus_tag="VIJ44_08175"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HEY5165912.1"
                     /translation="MADILKIADGLWRGELDTTQYHPFGHVDDLSEVGDRSGFVASFA
                     NVSAFDTDAGLVLVDSGSPVFSGAAHASLRRWTDAAVHTTVFTHGHIDHCFGVERYDA
                     DNAERSAPPVTVIAHEAVPVRFDRYRATGGFNTIINRRQFGLGPNFEFPTEYRYPDRT
                     YRDHLDLEIGGERFELHHARGETDDHTWVWIPSRQVLCTGDLFIWASPNCGNPQKVQR
                     YPKDWAVALRTMAALGAVTLLPGHGLPIVGADRVRQALQETAALLEYLHDSTVELMNQ
                     GATLDEIVHSVHAPPELLERPYLRPVYDEPEFVVRNTWRLYGGWYDGNPSRLKPAPDV
                     TVAREVAALAGGPGALA"
BASE COUNT          472 a         1035 c          970 g          448 t
ORIGIN      
        1 cgtagccgcg cacgagggga ccagcagcga gaccgccacg taggtgcggc agcacggcgt
       61 gacactcgac tcgttccgcc agttcgagcg cgacagcgca agccgcatga gcgtcggtcg
      121 tgacaaacat gacctcgtca ccgatggtct tgaccacccg accgtcgcta cccgagacca
      181 gctcggtggc gtcactctcg aacgtgctga tcagggcccc cagctctgcg ggcggaagcc
      241 gctccgcgag ggtcgtcgag cccacgaggt cgagaaaccc gacggtcaga tccgaggtgt
      301 cgaggttgcc cgaggcctgg gtgcgtcgcg tcgccgcctc gcaatggtgc atgaacaggc
      361 tggcgagtac gtgcgggacc tcgtcgaaga ggatcagcgt cgccgcttcg agcgcttggg
      421 tgcgcgccag ctcaccggcc tgttcggcgt cgagacgggg ggcgacgccc tgcccaaagt
      481 tcgtcatcgc ggcatcggcg acgctcgaga gcgcggctcc gaccacccgg gagaactgca
      541 gggccgattc gaccccgaac atctccgccg ccgcggcgaa cagacgaaat gtcacgatgt
      601 cagcctcgaa gaaggtcgcc tcgtcggccg ccgggtcgcc cagtccggcg gttcggttga
      661 tccgctggcc caactcgatc ggtatcccgg ctcgtcgggt cgcttctcga gtggtgagcc
      721 ggggccgacg acgctggagg agttcaccgg cgaggccggg aaggttcccg tcagacaccg
      781 cggtggcgag gtcgtcagtc gtcgcgcccc gttccgccaa gtagcggagg gtcgcaaggc
      841 ggtcggccgc ggtgagcgcg tcggggtcgt agagaccgcg ttcggcccac gtgctcgcgt
      901 cggcgtccac gccacgccac ggtatccagt cgctcgatgt accggggccg ctgccgcgcg
      961 gggatctccg ggcgccttca gaccgcgccg acgggcaagg gcagagacgg ccgcgatcgc
     1021 cgaccgccga gagcagtcgc gaggtgacgg ccggtagcat cgccgcgtgc gccatcgcca
     1081 tcgcgtcgtg ctcatcggag cggtggcact cgccacgctc gccgcggcgt gtggtggaag
     1141 tgggggcccg accctcgacc ggaaggcaac cgccgcgccg agcacgacac cggcgacgct
     1201 gccctcgttc atcagtctga tcgcccgggt cagcgtcccg aatgtcgcgg tctacgacgc
     1261 gcccacgtcg ccgacgccgt cacatacctt cgcgaacccg tggctgttga acgccgaccc
     1321 gaagcttccg gtccaactcg tcttcctcgt cgagcagcgc caggggccct ggctccaggt
     1381 gctgctcccg attcgaccca atggcacgac cggctgggtg cgcagccgag acgtgtccgt
     1441 gcagcagaac gagtttcgga tcacggtcga gcttcacgcg catcgcctcg tcgtctacaa
     1501 cggcgagtcg gtgttgctga gcgacacggt ggcggtgggc atgcctgcga ccccgacgcc
     1561 gacggggacg tatttcatcc gcgtcctcct caaggcaccc gatccgacca cggtctacgg
     1621 cccgtacgcg tacggcctct ctggtcattc cgaggcgctc aacacgttcg acggtgggga
     1681 cgccgagctc ggcattcacg gaaacaacga cgcgtcggtg ctcggcaccg atgcgagcca
     1741 cggctgcatc cgaatgagca acgacaagat caccatgttg gccggcgtcc tgcccctcgg
     1801 tacgccggtc cagatcaccg cctgaccggc gacgacctca ccggatccaa ctgcagtctg
     1861 agagggcacc gacatggctg acatactcaa gatcgctgac gggctctggc gaggtgagct
     1921 cgacaccacc cagtaccacc cgttcggcca cgtcgacgac ctcagcgagg tgggtgaccg
     1981 ttcgggcttc gtcgcgtcgt tcgcaaacgt cagcgccttc gacaccgacg ccggcctggt
     2041 cctggtggat agcggcagcc cagtcttctc gggcgccgcg cacgcgagcc tccgacgctg
     2101 gactgacgct gccgtccaca ccaccgtgtt cacgcacggt cacatcgatc actgcttcgg
     2161 cgtggagcgc tacgacgccg acaacgccga acggtccgct ccacccgtga ccgtcatcgc
     2221 ccacgaggcg gtccccgtac gtttcgatcg ctaccgcgcc accggcgggt tcaacacgat
     2281 catcaaccgc cgtcagttcg ggctgggccc gaacttcgag ttcccgaccg agtaccggta
     2341 cccggaccgc acctatcgcg atcacctcga cctggagatc ggcggagaac ggttcgagct
     2401 ccaccatgcc cgtggggaga ccgacgatca cacctgggtg tggatcccgt cccgccaggt
     2461 gctgtgcacc ggggacctgt tcatctgggc ttcgcccaac tgcgggaacc cccagaaggt
     2521 ccagcgctac ccgaaggact gggctgtggc gctgcggacc atggctgcac tcggcgcggt
     2581 gacgctgctc ccgggccacg gccttccgat cgtcggggcc gaccgggttc gccaggccct
     2641 ccaggagacg gccgcgctcc tcgagtatct ccacgactcg acggtggagc tgatgaacca
     2701 gggagcgaca ctcgacgaga tcgtccactc ggtgcacgct cccccggagc tgctcgagcg
     2761 gccgtacctt cggccggtct acgacgagcc ggagttcgtc gtgcgcaaca cgtggcggct
     2821 atacggcggt tggtacgacg gcaacccgtc gcgcctcaag ccggcacccg acgtgaccgt
     2881 cgcccgggag gtcgcggcgc tcgccggtgg ccccggggcg ttggc
//
LOCUS       DATCCH010000425         2713 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_241246, whole genome shotgun sequence.
ACCESSION   DATCCH010000425 DATCCH010000000
VERSION     DATCCH010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241246"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VIJ44_08180"
     CDS             <1..251
                     /locus_tag="VIJ44_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020385810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEY5165913.1"
                     /translation="NPVSDMPTWRLLVAPAKGTFRRGSNANLLVPGATVGVVVTLRDE
                     QPVMAPHGGHVVEWLVEDGDPVAPGQPLVRLHPEAVPV"
     gene            248..1258
                     /locus_tag="VIJ44_08185"
     CDS             248..1258
                     /locus_tag="VIJ44_08185"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEY5165914.1"
                     /translation="MTGAPTFKTAQGAPYARMLGVGGYRPARVIPNSEIVDRIDSSDE
                     WIRERSGIQTRHAAAADESVIDMALVASEKALANAGVTADQLGCVIVATVTHLMQTPS
                     AAAVLGHRLGTTKAAAFDISAACAGFCHALALANDMIRGGSAEYVLIVGVEKLSDMVD
                     PYDRGSAFIFGDGAGAAVVGPSDVPGIGPVVWGADGGQANAITQDVPWHTLRDDPTHK
                     FPTLSMNGQQVFRWAVFQMAPVCQEALDRAGVRADQLDAFIPHQANLRIVEAMVKKIG
                     LPDNVAVARDIVDTGNTSAASVPLAMERLLDNGEAPHGGTALLIGFGAGLSFAAQVVT
                     LP"
     gene            1351..1596
                     /locus_tag="VIJ44_08190"
     CDS             1351..1596
                     /locus_tag="VIJ44_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018556746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEY5165915.1"
                     /translation="MATQQEILSGLADIVNEVAGVPVADVQLDKSFVDDLDIDSLSMV
                     EVVVAAEEKFDVKIPDDDVKGLKTVGDAVSYIAAAGA"
     gene            1690..>2713
                     /locus_tag="VIJ44_08195"
     CDS             1690..>2713
                     /locus_tag="VIJ44_08195"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017945076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEY5165916.1"
                     /translation="MTTPLGGDVPTTWAAMLAGTPGGRTLTEPWAEDLPVRFAATALV
                     DPLEVMDRVEARKLDRSEQFALIAAREAWADAGTPEVDPLRLGVVVASGIGGAVSLLN
                     AWDTMREKGARRVSPHTIPALMPNGPAGYVGIEFKARAGVHTPVSACASGAEAVTYAV
                     HMIRSGRADVVIAGGTEACIHPLPMAAFAAMMALSKRNDEPGRASRPYDKGRDGFVLG
                     EGAGVLILESAEYAAARGARVYGIVAGSGITSDGHHIAQPDPVGAGAKRAIEFALADA
                     GATPADVVHANAHATSTPQGDVAEASAIRAVLGLDADHVAVSATKSMTGHLLGAAGAI
                     ESIATVL"
BASE COUNT          400 a          997 c          948 g          368 t
ORIGIN      
        1 ccaaccccgt ctccgacatg ccgacctggc gccttctggt cgccccggcc aagggcacct
       61 tccgccgcgg cagcaacgcc aacctcctcg tacccggcgc cacggtcggg gtcgtcgtca
      121 ccctgcgcga cgagcagccc gtcatggccc cgcacggcgg gcacgtcgtc gagtggctcg
      181 tcgaggacgg cgacccggtc gctccgggcc aaccgctggt gcgcctccac cccgaggcgg
      241 tgccggtatg actggggcgc ccacgttcaa gacggcccag ggcgctccgt acgcccggat
      301 gctcggtgtc ggcggctacc gcccagcccg cgtgatcccc aactccgaga tcgtcgaccg
      361 catcgactcc tccgacgagt ggatccgcga gcgctccggc atccagacac ggcacgcggc
      421 cgcggccgac gagtcggtca tcgacatggc gctcgtcgcg tccgagaagg cgctggcgaa
      481 cgcaggcgta acggccgacc agctcggctg cgtgatcgtg gccacggtca cccacctgat
      541 gcagacgccg tcggcagcgg cagtcctcgg tcaccggctc ggtacgacca aggcggccgc
      601 cttcgacatc tcggctgcct gcgccgggtt ctgccacgcc ctcgcgctgg ccaacgacat
      661 gatccgaggt ggctccgcgg agtacgtgct catcgtcggg gtcgagaagc tcagcgacat
      721 ggtcgacccg tacgaccgcg gctcggcgtt catcttcggc gacggcgcgg gtgcggccgt
      781 ggtcggcccg tccgacgtgc ccggcatcgg gccggtggtg tggggcgccg acggcgggca
      841 ggccaacgcg atcacgcagg acgtgccctg gcacaccctg cgcgacgacc cgacgcacaa
      901 gttcccgacg ctgtcgatga acggccagca ggtgttccgc tgggcggtct tccagatggc
      961 gccggtctgc caggaggccc tggacagggc gggcgtgcgc gccgaccagc tcgacgcgtt
     1021 catcccgcac caggccaacc tgcggatcgt cgaggccatg gtgaagaaga tcgggctgcc
     1081 cgacaacgtg gccgtcgcgc gggacatcgt cgacacgggc aacacgtcgg cggcgtccgt
     1141 cccgctcgcc atggaacggc tgctcgacaa cggcgaggcg ccgcacggcg gcaccgcgct
     1201 cctgatcggc ttcggtgcgg gcctgtcgtt cgcggcgcag gtggtcaccc tcccgtgatc
     1261 ggcgtttgcg cctcgggccg atagcctgca acgaccacac cgcagcaagc ggtgggaccg
     1321 aacaatcccc tgacgaagga gtcccccagc atggccaccc agcaggagat cctctccggc
     1381 ctcgccgaca tcgtgaacga ggtcgcgggc gtcccggttg ccgacgtcca gctcgacaag
     1441 tcgttcgtcg acgatctgga catcgactcg ctgtcgatgg tcgaggtcgt cgtcgcagcc
     1501 gaagagaagt tcgacgtcaa gatcccggac gacgacgtca agggcctcaa gaccgtcggc
     1561 gacgccgtgt cctacatcgc ggcagcgggc gcctgagcgg cgtccgccca ccgaaccacc
     1621 cagaccgcta ggagggccgc agccgtgggc cagcagatcg acaccgatgt cgtggtgaca
     1681 gggctcggga tgacgacgcc gctcggcggt gacgtcccga ccacctgggc agccatgctc
     1741 gccggtaccc ccggtggccg caccctcacc gagccctggg ccgaagatct gccggtacgt
     1801 ttcgcggcga ccgccctggt cgacccgctc gaggtgatgg accgtgtcga ggcacgcaag
     1861 ctcgaccgga gcgagcagtt cgccctgatc gccgccaggg aagcctgggc cgacgccggg
     1921 accccggagg tcgacccgct gcgcctcggc gtggtggtcg cctccgggat cggcggcgcg
     1981 gtcagcctgc tcaatgcatg ggacaccatg cgcgagaagg gcgcccgacg ggtgtccccg
     2041 cacacgatcc cggcgctgat gcccaacggg ccggccggct acgtcggcat cgagttcaag
     2101 gcgcgggccg gcgtccacac tcccgtctcc gcgtgcgcgt ccggcgccga ggccgtcacg
     2161 tacgcggtgc acatgatcag gtcggggcgc gccgacgtgg tgatcgccgg cggtaccgag
     2221 gcctgcatcc acccccttcc gatggccgct ttcgcggcga tgatggcgct gtccaagcgc
     2281 aacgacgagc cgggcagggc gtcccggccg tacgacaagg gccgcgacgg gttcgtgctc
     2341 ggcgagggcg ccggtgtcct gatcctcgag tcggccgagt acgccgcggc gcggggggca
     2401 cgggtctacg gcatcgtcgc aggatcgggc atcacgtccg acgggcacca catcgcgcag
     2461 cccgacccgg tgggcgccgg ggccaagcgg gccatcgagt tcgcgctcgc cgacgcgggc
     2521 gccactccgg ccgacgtcgt ccacgccaac gctcacgcga cctcgactcc gcagggcgac
     2581 gtggccgagg cctcggcgat ccgggccgtg ctcgggctcg acgccgacca cgtcgccgtc
     2641 tccgcgacga agtcgatgac cgggcacctg ctgggcgcgg cgggcgccat cgagtccatc
     2701 gcgaccgtgt tgg
//
LOCUS       DATCCH010000426         5434 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_27035, whole genome shotgun sequence.
ACCESSION   DATCCH010000426 DATCCH010000000
VERSION     DATCCH010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5434
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27035"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VIJ44_08200"
     CDS             <1..270
                     /locus_tag="VIJ44_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HEY5165917.1"
                     /translation="SSNYAVGYARCTSPAGPCTKPHNGPLLGSGPQGAGPGGPDTFVD
                     QTSQLWLVYHAWDPAAVGTAANGRRTMRLSRLDLSGAAPILVAGP"
     gene            291..1430
                     /locus_tag="VIJ44_08205"
     CDS             291..1430
                     /locus_tag="VIJ44_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165918.1"
                     /translation="MTACAVIAVAGAIGAIATLSSGKASHRSVVAAQPAQSATPPGCT
                     AGAAAPSPPPAQPVAPAAGVTVPSANLIDTKGLVPGAVTETTDGAAWAIVRDPRATQP
                     DGMIARIDVGTHALTEITPVVTGCTATALAAHGTSLWVGTCNPAATGDTTGGAELIRV
                     DSAGQIDARIALPTSCVDAVAVGDTTTWVTSAPRSATAPRLFRVDQATGHVDAVPISA
                     GEQLTGIATLGDDLWSARTSAAGSRLVRTDAHSSAETASVATGAVRLLGISGTDLWTE
                     DNQHSALDTHDAHTGALTATVPIPNLQAATIGASGVWFEQATTTSLRITIGFVAGTVA
                     APVVTFTGAGPDRTGLPFLGALTATAHGAWLATQDQLFVIGPPRP"
     gene            complement(1494..1754)
                     /locus_tag="VIJ44_08210"
     CDS             complement(1494..1754)
                     /locus_tag="VIJ44_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165919.1"
                     /translation="MFDLCQEPLPSIDSRPEPAQVAQLALTRAASARVQWSRATLVSL
                     LTPSERQPPLPDLSGKDYLFVVESALRTTPAYQQAQVAARSG"
     gene            complement(1777..3774)
                     /locus_tag="VIJ44_08215"
     CDS             complement(1777..3774)
                     /locus_tag="VIJ44_08215"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEY5165920.1"
                     /translation="MVAHRLGIRRWPRPVAVLTLLVVGVVGSAFLGGIAAAQTTPTSS
                     VCDLIPDLSVRQQCRNALAGLPTAPTLPTGAIPSAPTISGVEHIASYGVALHVEATGD
                     LLVREQIRYDFGVVPHHGIFRDIPVRFDYPKRDATDRVYPLTVVSVAASPGTPAQYTT
                     ETYSTNGVGYERIKIGDPNRTISGEHLYTIAYRVRGVLNGFANHDEMVWNAVGGQWTV
                     PIQDVSVRVDTPAAITAVNCNEGPTGSTLPCASAQATGTTGSFTQPSLDPMAGVTVTV
                     AIPTGAVPTPKPILEQRRTLGRAFAVTPVTAGVSSGLLVLFIAGFLIVMWLFGRDRRY
                     RGSAVDQSFGTSDGTDERAPLFEHTETPVEFTPPDDLRPGQVGTLVGFRAHPLDVTAT
                     IIDLAVRKYLVIEEVDPENHARWLKKDWKLTKLKAADDQLKPYERQLFNGLLKGGDHT
                     NLSALRDHFATTMNKVESELLDDAMQQGWFRRKPQSLWLTVGAIGVLVLAAGIGLTIA
                     LALTTHAALIGIPVIVLGLLVTVGARWAPARTAKGSAVLRRVNGFRRFINESEKDRAR
                     FAEQKQLFSEYLPYAIVFGATDQWAKAFAGLAGDPADTSSWYRSSVPFEYLAFSNVIH
                     GFTVTASGTLTSTPPSTSGSSGFGGGGFSGGGAGGGGGGSW"
     gene            complement(3900..5297)
                     /locus_tag="VIJ44_08220"
     CDS             complement(3900..5297)
                     /locus_tag="VIJ44_08220"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY5165921.1"
                     /translation="MAGVRRWAPLVGALAVAATLLVAGGADAAAPSPVGAAGDWPQLA
                     HDNRSTFQNLGAGITPASARRLRVVWRWSAPGPVNGTPAVVGGHVYVVSLQGTASLDP
                     RNGQVQWLNRAATGTSSPTVRDQRVYVLDGGTTLHCLDASTGTEIWKTKVDAQQYANA
                     YSSPVVSGRLVIVGIASVAELAAQNPVTFRGSVVAFDRTTGAVVWRHATVEPPANGVG
                     VWSSVSVDEVTGTVFASTGNNYTTAGPTSDAIVAMDLTTGGTRWVRQVDTGDVFTLPH
                     PQSADSDFGTNPVLFDTTIGGKVRHLLAAGQKSGRFWVLDRISGAVVWHRTVSTGSAL
                     IGGVFNNGASDGHRLVLAGNDGGPGSARVGEPPAAHSSELVALNPANGRVLWRRHLDG
                     WVWAPITLSQRVGFVAVETEMQAFDISTGRLLARFPTEGTITSGPALSGGRAFFGSGL
                     SYFGTKPGRTFYALG"
BASE COUNT          794 a         1901 c         1993 g          746 t
ORIGIN      
        1 agctccaact acgcggtcgg ctacgcgcgg tgcacctccc ccgccggtcc gtgcacgaag
       61 ccgcacaacg gtccgctgct cgggtcgggg ccacaaggcg ccgggcccgg cggtcccgac
      121 accttcgtcg accaaaccag ccagctctgg ctcgtctatc acgcctggga ccccgccgcg
      181 gtcggcaccg cggcgaacgg ccgtcgcacc atgcgcctca gccgcctcga cctcagcggc
      241 gcggccccga tcctggtggc gggaccgtga gccacgcccg gcggcgaatc atgaccgctt
      301 gcgcggtcat cgcggtcgcg ggagcgatcg gcgcgatcgc caccctgagc agcgggaagg
      361 ccagtcaccg ctcggtggtg gccgcccagc ccgcgcagag cgccacaccg ccaggctgca
      421 ccgccggtgc ggcggcgccg agcccgccgc cagcccagcc ggtcgcgccg gccgcgggcg
      481 tcaccgtccc cagcgccaac ctcatcgaca ccaaaggcct cgtcccaggc gcggtcaccg
      541 agaccaccga cggcgccgcg tgggccatcg tccgcgaccc ccgcgccacc caacccgacg
      601 ggatgatcgc ccgcatcgac gtcggcaccc acgccctcac cgagatcacg cccgtggtga
      661 ccggctgcac cgccaccgcg ctcgccgcgc acggcaccag cctctgggtc gggacctgca
      721 atccggccgc gaccggtgac accaccggcg gcgccgagct catccgcgtc gacagcgcgg
      781 gccagatcga cgcgcggatc gccctcccaa cctcgtgtgt cgacgcggtc gccgtcgggg
      841 acacgacgac ctgggtgacc agcgcacccc gcagcgccac cgcaccccgc ctgttccgcg
      901 tcgaccaggc caccggacac gtcgacgcgg tcccgatcag cgccggcgaa cagctcaccg
      961 ggatcgccac cctcggcgac gacctctggt ccgctcgaac cagcgccgcg gggtcccggt
     1021 tggtccgcac cgacgctcac tcgagcgccg agaccgcatc ggtcgcgacc ggagcggttc
     1081 gactcctcgg catctccggg accgacctgt ggaccgagga caaccagcac tcggccctcg
     1141 atacacacga cgcccacacc ggcgcgctga ccgcgacggt cccaatcccg aacctgcagg
     1201 ccgccaccat cggagccagc ggcgtctggt tcgagcaagc gaccaccacc tcgctccgga
     1261 tcaccatcgg gtttgtcgcc ggcaccgtcg cggcgccggt ggtgacgttc accggcgcgg
     1321 ggcccgaccg caccggcctc ccgttcctcg gcgccctcac tgccaccgcc cacggcgctt
     1381 ggctcgccac ccaggaccaa ctgttcgtga tcggcccgcc caggccttga tcccccgcgc
     1441 ctcgctggcg cccagcgtcg ttcgcccgcg cctgtccgga tctgccagcg aggtcagccg
     1501 ctgcgggccg cgacctgcgc ctgctggtag gccggggttg tgcgcaacgc ggactccacc
     1561 acgaagagat agtccttgcc gctcaagtca gggagtggcg gttgacgctc ggatggcgtg
     1621 aggagcgaca ccagcgtggc tcgtgaccat tggacgcggg ccgatgcggc gcgggtgagc
     1681 gccagttggg ccacctgggc cggctcggga cggctgtcga tcgacgggag cggctcctgg
     1741 cacaggtcga acaccttgcc agggtttcgg gtcgggctac caggatccgc cgccgccgcc
     1801 gccggccccg ccgccagaga acccgccgcc gccgaacccg ctcgaccccg aggtggatgg
     1861 cggcgtggag gtgagcgtcc cggaggcggt gacggtgaac ccgtgaatga cgttcgagaa
     1921 cgccaggtac tcgaacggga cactggaccg gtaccaggag ctggtgtcgg cggggtcgcc
     1981 agcgaggcca gcgaacgctt tggcccactg gtcggtggcg ccgaagacga tggcgtaggg
     2041 caggtactcg gagaacagct gcttttgctc cgcgaaccgg gcccggtcct tctccgattc
     2101 gttgatgaag cggcggaagc cgttgacgcg tcgcagcacc gcggatcctt tggcggtgcg
     2161 ggcgggcgcc catcgggccc cgacggtgac gagcaggccg aggacgatga cggggatgcc
     2221 gatgagcgcg gcgtgggtgg tgagcgccaa cgcgatcgtg agcccgatcc cggcggcgag
     2281 gacgagcacg ccgatcgcgc cgacggtgag ccacagcgac tggggtttac gacggaacca
     2341 tccttgttgc atggcgtcgt cgagcagctc gctctcgact ttgttcatcg tggtcgcgaa
     2401 gtggtcgcgg agcgcggaga ggttggtgtg gtccccgcct ttgagcagcc cgttgaacag
     2461 ctgacgttcg tacggcttga gctggtcgtc ggcggctttg agcttggtga gcttccagtc
     2521 cttcttcagc caccgcgcgt ggttctcggg gtcgacctcc tcgatgacga ggtacttgcg
     2581 gaccgcgagg tcgatgatgg tggcggtgac gtcgagggga tgggcccgga acccgaccag
     2641 ggtgccgacc tggcccggcc gcaggtcgtc gggtggggtg aactcgaccg gggtctcggt
     2701 gtgctcgaac agcggggcgc gctcgtcggt gccgtcggag gtgccgaacg actggtcgac
     2761 cgcggacccg cggtagcgac ggtcacggcc gaagagccac atgacgatca ggaaccccgc
     2821 gatgaacagc accagcaggc cgctggaaac cccggcggtg acgggggtga cggcgaaggc
     2881 gcgccccagg gtccggcgct gctcgaggat gggcttcggg gtcgggacgg ccccggtcgg
     2941 gatcgcgacg gtgacggtga cgccggccat ggggtcgagc gacggctggg tgaacgaccc
     3001 ggtggtcccg gtcgcttgcg cgctcgcgca cggcagcgtc gagccggtgg gcccttcgtt
     3061 gcagttcacc gcggtgatcg cggccggggt gtcgactcgg acactgacgt cctggatcgg
     3121 caccgtccac tggccgccga ccgcgttcca gaccatctcg tcgtggttcg cgaagccgtt
     3181 gaggaccccg cggacccggt aggcgatggt gtagaggtgc tcgccgctga tggtccggtt
     3241 cggatccccg atcttgatgc gctcgtagcc gacaccgttg gtgctgtagg tctcggtcgt
     3301 gtactgggcc ggggtgcccg gcgaggccgc caccgagacg acggtgaggg gatagacccg
     3361 gtcggtggcg tcgcgcttgg ggtagtcgaa ccggaccggg atgtcgcgga agatgccgtg
     3421 gtggggaacg acgccgaagt cgtatcggat ctgctcgcgg acgaggagat caccggtcgc
     3481 ctcgacgtgg agggcgaccc cgtaggaggc gatgtgctcg acgccggaga tggttggcgc
     3541 ggacgggatc gccccggtcg ggagggtcgg tgccgtcggc aggccggcca acgcgttgcg
     3601 gcactgctgg cggacgctga ggtccgggat caggtcacag accgagctgg tcggtgtggt
     3661 ctgggccgcg gcgatgcccc cgaggaacgc ggaccccacg acaccgacga ccaggagggt
     3721 cagcaccgcg acgggtctcg gccaacgtcg gatgccgaga cgatgggcga ccatgcgcga
     3781 tcctcggttc tctgggacca cggcgatcgg gcggtcgcgc cggagtcgcg gttcgagcgt
     3841 cgggagcata aggcaccggc cgtcggttcg ggtcggggcg ggcgggtcgg gtgggcaggt
     3901 caacccaggg cgtagaaggt gcgaccgggc ttcgtcccga agtagctgag cccgctgccg
     3961 aagaaggccc gcccgccgga cagggccggc ccgctcgtga tggtgccctc ggtcgggaag
     4021 cgggccagca gccggccggt gctgatgtcg aaggcttgca tctcggtttc gacggcgacg
     4081 aagccgaccc gttgcgacag ggtgatcggg gcccagaccc acccgtcgag gtgtcgacgc
     4141 cacaggacgc gaccgttcgc ggggttgagc gcgacgagct cgctgctgtg cgcggcaggg
     4201 ggttcgccga cccgagccga gcccggcccg ccgtcgttgc cggcgaggac gagccgatgc
     4261 ccgtcggagg cgccgttgtt gaacaccccg ccgatcaggg cgctgccggt actcaccgtg
     4321 cggtgccaca cgacggcgcc gctgatccgg tcgaggaccc agaatcgccc cgacttctgt
     4381 cccgcggcga gcaggtgacg gaccttgccg ccgatcgtgg tgtcgaagag gacggggttg
     4441 gtgccgaagt ccgagtcggc tgactgcgga tgcgggaggg tgaagacgtc accggtgtcg
     4501 acctggcgga cccaacgggt gcctcccgtg gtcaggtcca tggcgacgat cgcgtccgag
     4561 gtcggtcccg cggtggtgta gttgttcccg gtggaggcga agacggtgcc ggtgacctcg
     4621 tcgacggaca ccgaggacca gaccccgaca ccgttcgcag gtggttccac ggtggcgtga
     4681 cgccagacga ccgcgccggt ggtgcggtcg aacgcgacga cgctgccccg gaacgtcacc
     4741 gggttctgcg ccgcgagctc ggcgaccgaa gcgatcccga cgatgacgag ccgccccgag
     4801 acgaccggtg acgagtaggc gttcgcgtac tgctgagcgt cgaccttcgt cttccagatc
     4861 tcggtgccgg tcgacgcgtc gaggcagtgg agcgtcgtgc ccccgtcgag gacgtacacc
     4921 cgctggtcgc gaaccgtggg tgacgacgtc cccgtcgcgg cgcggttgag ccactggacc
     4981 tgcccgttgc ggggatcgag cgacgcggtg ccctgcaacg acacgacgta cacgtggccg
     5041 ccgacaacgg ccggggtgcc gtttaccgga cccggggccg accaccgcca cacaacccgg
     5101 aggcgccgcg ctgacgcggg ggtgatcccc gccccgaggt tctggaacgt cgagcggttg
     5161 tcgtgggcca gctggggcca gtcccccgcg gcccccaccg gtgatggcgc cgcggcgtcg
     5221 gcgccgcccg ccacgaggag ggtcgccgcg acggccaacg cgccgacgag gggcgcccag
     5281 cgccgcacgc cggccacgcg gcactccaat cggtggcgca ccgctcagcc gagccgcgcg
     5341 agctcggctc gcgaccgggc cgcgaaccgc ggggcccggg cgcattcgga caattccacg
     5401 gcgcggcgga ggagctcctc acctcggtat cggt
//
LOCUS       DATCCH010000427         3893 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_241506, whole genome shotgun sequence.
ACCESSION   DATCCH010000427 DATCCH010000000
VERSION     DATCCH010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3893
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241506"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1681
                     /gene="rho"
                     /locus_tag="VIJ44_08225"
     CDS             <1..1681
                     /gene="rho"
                     /locus_tag="VIJ44_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019876280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEY5165922.1"
                     /translation="GVKAPTRMRKSELIDAILDAAESGGGTTGNGDEPADPSSPAVPA
                     STSDEGDGRPRTIRSARASELERDSVAALVAEEEALPSGDAAEDSVVAPRPARSAADS
                     DASGTKPTTGSDDRDATDTDAPGTDAPGTDVPGTDSEPPGEFDDERASYGDGNSRRRR
                     RRRGRGGPEGEPRSPEPAGEPVEVNGLLELRDEGYGFLRTTGYLAGARDVYVSASQVR
                     RFALRKGDFLHGFSRPQASNEKYPALLRVDHINNMTPDEARERPRFEDLTPLFPDERL
                     RLELPDNPLETTGRIVDLISPIGKGQRGLIVSPPKAGKTTILKQLVYSIERNNPEVHL
                     IVLLVDERPEEVTDMRRHVTRGDVVSSTFDRPAEEHTIVADLAIERARRLVELGTDVV
                     VVLDGITRLARAYNLAAPASGRVLSGGVDSTALYPPKKFFGAARNIEEGGSLTIMATA
                     LIETGSRMDEVIFEEFKGTGNMELRLDRRLAERRIYPAIDVEASSTRHEELLFERNQL
                     NQVWKLRRVLNALDGDSSGGKGLELLLDKLRTTKSNDEFLAEIAKAPTIAG"
     gene            1756..1965
                     /gene="rpmE"
                     /locus_tag="VIJ44_08230"
     CDS             1756..1965
                     /gene="rpmE"
                     /locus_tag="VIJ44_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEY5165923.1"
                     /translation="MKTDIHPEYVTCTVHCSCGNEFTTRSTVPSLRVELCSECHPFYT
                     GKQKLVDTGGRVERFQRRAAKAKKS"
     gene            1971..2897
                     /locus_tag="VIJ44_08235"
     CDS             1971..2897
                     /locus_tag="VIJ44_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HEY5165924.1"
                     /translation="MPAPRYMGGQAVMEGVMMRGEHSWAVAVRTPDGDIELAVHDAPR
                     WGEQWSKIPVVRGVVALAESLALGFRALSWSAERQLPEEERISQRAMGWTIAVALVFF
                     TAVFIVLPTLINQGVADTLGVGGLTYHLAEGAIRLAVFLGYLTLIGFLPDIKRVFQYH
                     GAEHKAIAAYENGDELTPESAQRYTTEHVRCGTNFLLTVMVVTIFVYAFVGRPALPWL
                     ITSRILLIPVIAGLAYELIRAAAPRMDRRWVRVLMRPGLTLQRLTTRPPTRDQLAVAI
                     TSLRAVMTVEQLADVDARALVPAAPSPAFGTA"
     gene            3456..>3893
                     /locus_tag="VIJ44_08240"
     CDS             3456..>3893
                     /locus_tag="VIJ44_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011893284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY5165925.1"
                     /translation="MRFGLFYEHQLPRPWTDDSEFELLQNALEQCELADTLGIEYVWE
                     VEHHFLEEYSHSSAPEVFLAAVSQRTRNLRLGHGIVQTPPPFNHPARVAERVAMLDLV
                     SDGRVDFGTGESSSEAELGGFLIDPDEKRAMWEEGLRVALRCMT"
BASE COUNT          650 a         1400 c         1270 g          573 t
ORIGIN      
        1 gggcgtgaag gcacccaccc ggatgcgcaa gtccgagctg atcgacgcca tcctcgacgc
       61 cgccgagagc ggcggcggca ccaccggaaa cggtgatgag cccgccgacc cgtcatcacc
      121 ggctgtccca gcgagcacct ccgacgaggg cgacggccgg ccccgcacca tccgatcggc
      181 acgagcttcc gagctcgaac gggactcggt cgccgcgctc gtcgccgagg aggaagccct
      241 tcccagcggc gacgccgccg aggattccgt ggtcgcccct cggccggccc gcagtgccgc
      301 cgacagcgac gccagcggta ccaagccgac gacgggatcc gacgatcgcg acgcgaccga
      361 caccgacgca cccggcaccg acgcacccgg caccgacgtg cccggcaccg actcggagcc
      421 gccaggcgag ttcgacgatg aacgggcctc gtacggtgac ggtaactcgc gtcgccgacg
      481 ccgccgccgg ggtcgaggcg gccccgaagg cgagccccga tccccggagc ctgcgggcga
      541 accagtcgag gtcaacgggc tgctcgagct ccgcgacgag ggctacgggt tcctgcgcac
      601 caccggctac ctggcaggcg cgcgcgacgt gtacgtctcc gcgtcccagg tgcggcgatt
      661 tgcgctccgc aagggcgact tcctccacgg cttctctcgc ccgcaggcat ccaacgagaa
      721 gtacccggcg ttgctgcgcg tcgaccacat caacaacatg acgcccgacg aggcccgtga
      781 gcgaccccgg tttgaggacc tgaccccgct cttccccgac gagcggctcc gcctcgagct
      841 gcccgacaac ccgttggaga ccaccggccg gatcgtcgat ctcatctcgc cgatcggcaa
      901 gggacagcgg ggactgatcg tctcgccgcc gaaggccggc aagaccacga tcctcaagca
      961 gctcgtgtac tccatcgagc gcaacaaccc tgaggtgcac ctgatcgtgc tcctcgtcga
     1021 cgagcgtccc gaggaagtga cggacatgcg ccgccacgtg acgcggggtg atgtcgtctc
     1081 cagcaccttc gaccggcccg ccgaggagca caccatcgtc gccgacctgg cgatcgaacg
     1141 agcaaggcgc ctggtcgagc ttggcaccga tgtggtggtc gtcctcgatg gaatcacccg
     1201 acttgcccgg gcgtacaacc tggcggcgcc ggcgtcggga agggtcctct cgggtggcgt
     1261 cgactccacc gcgctgtacc cgcccaagaa gttcttcgga gccgcccgca acatcgaaga
     1321 gggcgggtcg ctgaccatca tggcgaccgc cctcattgag accggcagcc ggatggacga
     1381 agtgatcttc gaggagttca aaggcaccgg gaacatggag ctccgcctcg accggcgcct
     1441 ggctgagcgg cggatctacc ccgccatcga cgtcgaggcg tcgagcaccc gccacgagga
     1501 gctgctcttc gaacgcaacc agctcaacca ggtgtggaag ctgcggcggg tcctcaacgc
     1561 cctcgacggt gacagctcgg gtggcaaagg cctcgagctg ctcctcgaca agctccgcac
     1621 caccaagagc aacgacgagt tcctcgccga gatcgccaag gctcccacaa tcgcggggtg
     1681 agccgccgcc gctcgatcac gaaccgccgc ccgggcgatc cgaccaccgt tgggtcacgg
     1741 tagattggta ggaccatgaa gaccgacatc caccccgagt acgtcacctg caccgtccac
     1801 tgctcgtgtg gcaacgagtt cacgacccgc tcgacagtgc cgtcgctacg cgtcgagctg
     1861 tgctccgagt gccatccctt ctataccggc aagcagaagc tcgtggacac cggcgggcgg
     1921 gtcgaacgct tccagcgccg agccgccaaa gccaagaagt cctagcgccc atgcccgcgc
     1981 cccgatacat gggcggccaa gccgtcatgg agggcgtgat gatgcgcggc gagcacagct
     2041 gggccgtcgc cgtccgtacg ccggacggcg acatcgagct cgcggtgcac gacgcgccgc
     2101 gctggggtga gcagtggtcc aagatccccg tcgtgcgagg cgtcgtggcg ctcgccgaat
     2161 cgctggccct gggattccgg gccctctctt ggtcagcgga gcgtcagctc cccgaggagg
     2221 agcgcatctc gcaacgggcg atgggatgga ccatcgccgt cgcgctggtg ttcttcaccg
     2281 cggtgttcat cgtcctgccc accctcatca accagggtgt cgccgacacg ctcggcgtgg
     2341 gagggctcac ctaccacctc gcggagggag ccatccgact cgcggtcttc ctcgggtacc
     2401 tcaccctcat cgggttcttg cccgacatca aacgggtctt ccagtaccac ggcgccgagc
     2461 acaaggcgat cgccgcctac gagaacggtg acgagctcac ccccgaatcg gcgcagcggt
     2521 acacgaccga acatgtccga tgtgggacga acttcttgct gaccgtcatg gtcgtgacga
     2581 tcttcgtcta cgccttcgtg ggccgtccgg cgctcccgtg gctcatcacg tcacggatcc
     2641 tgctgattcc cgtcatcgcc gggctcgcct acgagctgat ccgagccgca gcgccccgca
     2701 tggatcgacg ctgggtccgg gtcctcatgc gcccgggact gaccttgcag cgtctgacga
     2761 cacggccccc gacccgcgat cagctcgcgg tggcgatcac ctcgctgcgg gccgtgatga
     2821 cggtcgagca gctggccgat gtcgacgccc gcgctctggt ccccgcagct ccgagccccg
     2881 ccttcgggac ggcctagacc tcgcaccgac cgggagctag tgcccctgcc cgtcgcttcg
     2941 ttgtcgacgg gcgtgcccac acgcgcggga cggcgcgtcg aaccaacggc ccgacccgtt
     3001 tgctggccag ggcacgtcgg taggccggct gggcggcgaa gagcgcggtg accgcctgga
     3061 aggggcagcg ggtcatccca ggcgaaccag gctcgctcat cgacccgcag cacggaccgc
     3121 atccatcgcg agaatcgcag gccctcgcgt tggtgatcgt cgcgatacgc cccgacctcg
     3181 aagggctcga ccgaccatga gatcctcggc ccgcgcgacg ctcgccgctt ccaggcgctc
     3241 gagcatgaga accggtttcc cgactcttga cgaccgcccg gagcccgaga tcggcgacgt
     3301 agcgagcgag gtcgtcgcgc cctcgaggga ccaggaccct cggggtcggg atccgatgct
     3361 cctcgcaggc acggtggagc tgcgcaccca ccgcgacgcg ccactcgccg atcgagaccg
     3421 ggccggtcga gaacgggtgg cactatcctg gcccggtgcg gttcggactg ttctacgagc
     3481 atcagctccc gcgcccttgg actgacgact ccgagttcga gctgctccag aacgcgctcg
     3541 agcagtgcga gctcgccgat acgttgggga tcgagtacgt ctgggaggtc gagcaccatt
     3601 tcctcgagga gtactcccac tcgagcgcac cggaggtgtt cttggctgcg gtctcgcagc
     3661 ggacccgcaa tctgcggctg gggcacggca tcgtccagac gcctccgccg ttcaaccatc
     3721 cggcgcgggt cgcggagcga gtggcgatgc tcgacctcgt ctctgacggc cgcgtcgact
     3781 tcgggaccgg cgagtcgtcc tcggaggcgg aactcggcgg gtttctcatc gacccggacg
     3841 agaagcgcgc catgtgggag gaagggctgc gtgtcgcgtt gcggtgcatg act
//
LOCUS       DATCCH010000428         2884 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_449813, whole genome shotgun sequence.
ACCESSION   DATCCH010000428 DATCCH010000000
VERSION     DATCCH010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449813"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1206)
                     /locus_tag="VIJ44_08245"
     CDS             complement(<1..1206)
                     /locus_tag="VIJ44_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012948208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY5165926.1"
                     /translation="MRVSLTVNDFLDRATLVYGDRCGIIDEPDQPATPLGSLTYREVG
                     ELAAAQAAGLDALGVGVGERVAIVSHNAARLLVALFGVSGSGRTLVPINFRLNADEIG
                     YIVEHSGATVLLVDPELDPVLAGVAAKHRFVLGADDPEWVRFGVEPERWEPDEDATAT
                     INYTSGTTARPKGVQLTHRNVWINAVTFGWHLGVTDRDVYLHTLPMFHCNGWGMPYAV
                     TGMGATHVVLRRVDGAEILRRVEHHGVTALNGAPTVVNMVLDAARDWPGEIPGRGRVR
                     ITVAGASPPTRTIERVETELGWEFVQIYGLTETAPLLTMNRSRAEYDDLDPATRATRL
                     ARAGAPVVGVRIQVDPDGEVQARSNVVMDGYWNQPEDTARAIRDGWFHTGDGGHIDED
                     HYLVISDRKM"
     gene            1470..1829
                     /locus_tag="VIJ44_08250"
     CDS             1470..1829
                     /locus_tag="VIJ44_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165927.1"
                     /translation="MIHVPTDASTIQRAVDQAKPGTLVLVAAGVYHEAVSVTHSNIVI
                     RGESRTGTIVDCAFSTDGTKANAFEVTANGVAIENLTARNTVDVARVGVPPGPPAGWR
                     ARPASGEDRGRASARAR"
     gene            complement(1810..>2884)
                     /locus_tag="VIJ44_08255"
     CDS             complement(1810..>2884)
                     /locus_tag="VIJ44_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011604879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HEY5165928.1"
                     /translation="ATLTDLRERLARTRLPNQIAGIGWDQGTELGVLTDLLGSWRDQY
                     DWRRVEAELNGYEHIQTSIDGQRLHALHARSGSPDALPLLLVHGWPGSIVEFLDALPA
                     LTETFHVVAPSLPGYGFSGPTTTPGWNPRRIAHACTDLMAALGYDRYGAQGGDWGSIV
                     CANVADVDPDHVIGLHLNFVIVPHPADAPPLTPEEQRALAALGDWSRDEVAYQQIQGT
                     RPQTLGYSLEDSPSGLGAWIIEKFRAWSGGGDALDTFSTDRLLDNITLYWVTRTATSS
                     TRLYWEMRRAGRDALPQSRVEVPTGIANYPAELTRMPRAWVEHRYNVTHWVDQPRGGH
                     FAAMQVPDLFVEDLRAFFTSLGH"
BASE COUNT          442 a          975 c         1041 g          426 t
ORIGIN      
        1 catcttccgg tccgagatga cgagatagtg gtcctcgtca atgtgaccac cgtccccggt
       61 gtggaaccag ccgtcccgga tcgctcgggc cgtgtcctcg ggttggttcc agtagccgtc
      121 catcacgacg ttggagcggg cctggacctc accgtcggga tccacctgga tgcggacccc
      181 cacgacaggt gcaccggcgc gggccaaccg ggtggcgcgc gtcgcagggt cgaggtcgtc
      241 gtactcggcg cgggaacggt tcatcgtgag gagcggtgcc gtctcggtca gcccgtagat
      301 ctggacgaac tcccaaccga gctcggtctc cacccgctcg atcgtccggg tgggcgggga
      361 ggcgccggcg accgtgatcc ggacccgacc tcgtcccggg atctcgccgg gccagtcgcg
      421 ggccgcgtcc aggaccatat tcacgaccgt gggcgccccg ttgagcgccg tcacgccgtg
      481 gtgttcgact cggcggagga tctcggcccc gtcgacccgt cgcaagacca cgtgcgtcgc
      541 acccatcccg gtgacggcgt acggcatccc ccagccgttg cagtggaaca tgggcagcgt
      601 gtggaggtac acgtcgcggt cggtgacgcc cagatgccag ccgaacgtca ccgcgttgat
      661 ccacacgttg cggtgggtga gctggacccc cttggggcgg gccgtggtgc cactggtgta
      721 gttgatggtc gcggtcgcgt cctcgtcggg ctcccaccgc tcgggttcaa cgccgaagcg
      781 cacccactcg ggatcgtcag cgccgaggac gaaacgatgc ttggcggcga cgcccgcgag
      841 gaccgggtcg agctcggggt cgacgagcag aacagtcgct ccggagtgct cgacgatgta
      901 gccgatctcg tcggcgttca gccggaagtt gatcggaacc aaggttcggc ccgagcccga
      961 cactccgaac agcgcgacga gcaatcgggc cgcgttgtgc gacacgatcg ccacccgctc
     1021 cccaacaccg acaccgagcg cgtcgagccc ggcggcctgc gctgctgcca gctcccccac
     1081 ttcccggtag gtgagtgatc cgagcggtgt cgcgggctga tcgggctcat cgatgatccc
     1141 gcaccgatcg ccgtagacga gcgtcgcccg atcgaggaag tcgttgaccg tcaacgaaac
     1201 gcgcatgcgg cagctccccc cggcgcggcg aacgagtgcc gcgcaaccta gtcggcgtgc
     1261 tccgctcccc caagcgaatc ccgagcggtc caagctcgac cgccagtggt tagggctcgc
     1321 ggagcagccg cgggcatcgt ggcggaggtc atgacggagg tggggatgcg cacacagcgt
     1381 cgggtgctcg gcgtcgtcgc gatcgtggtg ctcgtggcga gcgcgctcgg cggcgtcgcc
     1441 agcgcgtccg cgccccgcgc cagggcacgg tgatccacgt cccgaccgac gcgtcgacga
     1501 tccagcgcgc cgtcgaccag gccaagcccg gcacgctggt cctcgtcgcg gcgggcgtgt
     1561 accacgaggc cgtctccgtc acgcactcga acatcgtgat ccgcggcgag agtcggacgg
     1621 ggacgatcgt cgactgtgcc ttcagcaccg acggcaccaa ggcgaacgcc ttcgaggtca
     1681 ccgccaacgg cgtcgcgatc gagaacctaa cggcgcgcaa cacggtcgac gtggctcgcg
     1741 tcggggtgcc gccggggcca ccagctggct ggcgggcccg accggcttcc ggtgaggacc
     1801 gcgggcgggc tagtgcccga gcgaggtgaa gaaggcgcgg aggtcctcga cgaagaggtc
     1861 ggggacctgc atcgccgcga agtgcccgcc gcggggctgg tcgacccagt gggtgacgtt
     1921 gtaccggtgc tccacccacg cgcggggcat gcgggtgagc tcagcgggat agttggcgat
     1981 cccagttggc acctcgaccc gggactgcgg gagcgcgtca cgacccgctc ggcgcatctc
     2041 ccagtagaga cgggtcgacg aggtcgcagt tcgggtcacc cagtagaggg tgatgttgtc
     2101 gagcagccga tccgtcgaga acgtgtcgag cgcgtcgccc ccgcccgacc aggcccggaa
     2161 cttctcgatg atccacgcgc cgagaccgct cggtgagtcc tcgaggctgt acccgagggt
     2221 ctggggtcgg gtgccctgga tctgctgata cgccacctcg tcgcgggacc aatcgccgag
     2281 ggcggcgagc gcccgctgtt cctcgggggt cagcgggggc gcgtcggcgg ggtggggcac
     2341 gatgacgaag ttcaggtgca gcccgatgac gtggtcgggg tcgacgtcgg cgacgttggc
     2401 gcacacgatc gacccccagt cgccgccctg agcgccgtag cggtcgtagc cgagcgcggc
     2461 catgaggtcg gtgcacgcgt gagcgatgcg ccggggattc cagcccgggg tcgtggtcgg
     2521 tcccgagaac ccgtagccgg gcagcgaggg tgcgacgaca tggaacgtct cggtcagggc
     2581 tgggagcgcg tcgagaaact cgacgatcga gcccggccag ccgtgcacga gcagcagcgg
     2641 cagcgcatcg ggagagccgg aacgggcgtg gagcgcatgc agtcgctgcc cgtcgatcga
     2701 ggtctggatg tgctcgtagc cgttgagctc ggcctccacc cgccgccagt cgtactggtc
     2761 tcgccacgac cccagcagat cggtgaggac acccagctcg gtgccctggt cccaaccgat
     2821 cccggcgatc tggttgggca gccgagtccg cgccagtcgc tcgcgcagat cggtgagtgt
     2881 cgcc
//
LOCUS       DATCCH010000429         3572 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_152091, whole genome shotgun sequence.
ACCESSION   DATCCH010000429 DATCCH010000000
VERSION     DATCCH010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3572
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152091"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="VIJ44_08260"
     CDS             complement(<1..424)
                     /locus_tag="VIJ44_08260"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5165929.1"
                     /translation="MSALDGKVALITGAARGVGEATARRFAVEGARVILGDVLDEPGR
                     AAAATIGGAARFVHLDVTSEDSWRDAVAGIRDDEGHLEVLVNNAGILRVGSLVDTSTE
                     TFLEVMGVNQLGPFLGMRTAAPLLIESSPAAIVNVSSTQ"
     gene            complement(429..3056)
                     /locus_tag="VIJ44_08265"
     CDS             complement(429..3056)
                     /locus_tag="VIJ44_08265"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5165930.1"
                     /translation="MGHVTLKNLFAKKLRLVLTSLAVVLGVAFMSGTFVLTDTLGSVF
                     DNLFTTTTKGVDAVVRGREPFKAQGRNANSTRPPVPDSLVPVVQRTPGVANAQGNLLR
                     YALVQDRNGQAIQNQAPAFGVAWYPVKTEVNKSLELVTRFKGARSTQPAALDEVALDV
                     QTANDGGYRIGDRVRIGFATTPPRLFRLSGVFRFGGSDQGLAGATLAAFVPATAQQVM
                     NPPTQQATWDQIDVRAANGLSETAARDHLNVALRRGGFRQYEAITGTTLAQEQSNNVK
                     NNLSFFNTFLLIFALVALFVGAFIIYNTFSITVAQRARELGLLRALGASGSQVVGSVA
                     LEAFVVGLLSSVLGLGLGILLVSPLEGLLSAFGISLPTGPVQIQARTIIVALVAGTLV
                     TFVSAIAPARRAARIPPIAALQDQASVPTSGRRRYAWGGAFTILGVAFLTYGLYGGGS
                     NAAVTVGVAAALVFIGVAMLSPLVARPVARLLTLPAERRHSVTGVLAQQNAMRNPRRT
                     ASTAAALMIGLALVSLISIFGASAKASFSDAIDNQTRADFILSPKNFLPFSSAAAQAV
                     RSGFAAISRHPATVVEFRSGTAEAGGQATAISGTSPDFQKVFAVPVQHFNQRGYATNG
                     VLIWKDVVGQHTCGADEHSVCRNGSTIAMRFPEGPAQTVPIVGVFTDRKALPSGDDYL
                     VSLNGWEQRFPQTLDSTVVILKPSDVSTGGAQRVVDSVAKQFGGIKAQNKAQFKDSQV
                     AQFNQILGLMYVLLAFAVFIALIGIVNTLALSIYERTREIGLLRAVGMTRVQLRRMIR
                     GEAVIVAVFGSLLGLVIGVGFGAAIVQSLKDQGIGLTVPVGQLVLFVGLTGLAGLLSG
                     WLPARRAARLDVLGAINAE"
     gene            complement(3067..>3572)
                     /locus_tag="VIJ44_08270"
     CDS             complement(3067..>3572)
                     /locus_tag="VIJ44_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006375255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5165931.1"
                     /translation="ASEKQLTLIRRDNIGFVFQSYNLIPTLNALENITLPMALAGRKP
                     DPAWLDHVIDIVGLRDRMSHRPTELSGGQQQRVAVARALVSRPNVIFADEPTGNLDSR
                     AGAEILSFMRQAVDDLGQTIVMVTHDPIGAGYADRVVFLADGRIVDEMAGPTADSVLD
                     RMKRIGD"
BASE COUNT          582 a         1139 c         1318 g          533 t
ORIGIN      
        1 cttgggtgga tgacacgttg acgatggcgg ccgggctcga ttcgatgagc agcggcgccg
       61 cggtgcgcat cccgaggaac gggccgagct ggttgacgcc catcacctcg agaaaggttt
      121 cggtgctcgt gtcgaccagc gaccccaccc ggaggatccc ggcgttgttg acgagcacct
      181 cgaggtgtcc ctcgtcgtct cggatcccgg cgacggcgtc acgccaggag tcctcggagg
      241 tcacgtcgag gtgcacgaag cgggccgcgc caccgatggt cgccgcggcg gctcgtcccg
      301 gctcgtcgag gacgtcaccg aggatgacgc gggcaccttc gacggcgaat cgtcgggcgg
      361 tggcctcgcc gacgccgcgg gccgcgccgg tgatcagcgc gacctttccg tcgagggcgc
      421 tcacccggtc attctgcgtt gatcgcgccg agcacgtcga ggcgcgcggc gcggcgcgcg
      481 ggaagccagc cggacaacag ccccgcgagg ccggtgagac cgacgaacag cacgagctga
      541 ccgaccggaa cggtgaggcc gatcccctgg tccttgagcg actggacgat cgcggccccg
      601 aacccgaccc cgatgaccag cccgagcagc gaaccgaaga ccgcgacgat caccgcctca
      661 ccgcggatca tcctgcggag ctgcacccgg gtcatgccga cggcgcggag caacccgatc
      721 tcgcgggtcc gctcatagat cgacagcgcg agggtattga cgatgccgat gagcgcgatg
      781 aacaccgcga aggcgaggag gacgtacatc aacccgagga tctggttgaa ctgcgccacc
      841 tggctgtcct tgaactgggc tttgttctgc gccttgatgc cgccgaactg cttcgccacg
      901 ctgtcgacga ctcgctgcgc cccgccggtc gagacgtccg acggcttcaa gatgacgacg
      961 gtgctgtcga gggtctgggg gaaccgctgc tcccagccgt tgagcgagac caggtagtcg
     1021 tcgcctgatg ggagcgcctt gcggtcggtg aacacgccga cgatgggaac cgtctgggcc
     1081 ggtccttcgg ggaaccgcat ggcgatggtc gacccgtttc ggcacaccga gtgctcgtcg
     1141 gcgccgcagg tgtgctggcc cacgacgtcc ttccagatga gcacgccgtt ggtggcgtag
     1201 ccgcgctggt tgaaatgctg gaccggcacc gcgaagacct tctggaagtc cggggatgtg
     1261 ccggagatcg cggtcgcttg acccccggcc tccgcggtgc cgctgcggaa ctcgaccacc
     1321 gtcgccgggt gtcggctgat ggccgcgaac ccggaccgga cggcctgagc ggccgcggag
     1381 ctaaagggca ggaagttctt cggcgagagg atgaagtcgg cgcgggtctg gttgtcgatg
     1441 gcgtcggaga acgacgcctt cgccgacgcg ccgaagatgg agatgaggct gaccagcgcc
     1501 aacccgatca tgagcgcggc ggcggtcgac gcggtgcggc gggggttgcg catggcgttc
     1561 tgctgggcga gcaccccggt gacgctgtgg cgacgctcgg ccggcagggt gaggaggcgg
     1621 gccaccggtc gggcgaccag cgggctgagc atggccacgc cgatgaacac gagcgcagcc
     1681 gcgacgccga cggtgaccgc ggcattcgag ccgccgccgt agaggccgta ggtgaggaac
     1741 gcgacgccga ggatcgtgaa cgctccgccc caggcgtagc ggcgacggcc cgaggtcgga
     1801 accgacgcct ggtcttgcag cgccgcgatc ggagggatcc gcgccgcgcg gcgtgccggg
     1861 gcgatggcgg atacgaacgt caccaacgtt cccgcgacga gtgcgacgat gatggtccgg
     1921 gcctggatct ggaccgggcc cgtcgggagg ctgatgccga aggcgctcag cagcccttcg
     1981 agcgggctca cgagcaagat gccgagcccg agtcccagca cggacgacag caaccccacg
     2041 acgaacgcct ccaacgcgac ggacccgacg acctgggagc cggacgcgcc caacgcgcgg
     2101 agcagcccca gctcccgagc tcgttgcgcc acggtgatcg agaaggtgtt gtagatgatg
     2161 aacgccccga cgaagagggc caccagggcg aagatcaaga ggaaggtgtt gaagaacgag
     2221 aggttgttct tgacgttgtt gctctgctcc tgggcgagcg tcgtcccggt gatcgcctcg
     2281 tactgccgga agccgccgcg gcgcagcgcg acgttcaggt ggtcacgagc cgcggtctcg
     2341 gacaggccgt tggcggcacg gacgtcgatc tggtcccacg tcgcttgctg ggtcggcggg
     2401 ttcatgacct gctgcgcggt ggcggggacg aacgccgcga gggtggcgcc ggccaggccc
     2461 tgatcgctcc caccgaagcg gaacacaccg gagaggcgaa acagccgcgg tggggtggtc
     2521 gcgaagccga tacggacccg gtccccgatc cggtagccac cgtcattggc ggtctgcacg
     2581 tcgagggcca cctcgtcgag tgcggccggt tgggtcgagc gggcaccttt gaagcgggtg
     2641 acgagctcga gcgacttgtt gacctcggtc ttcacgggat accaggcgac cccgaacgcg
     2701 ggcgcctggt tctggatggc ctgcccgttg cggtcctgca ccagcgcgta ccgcagcaag
     2761 ttcccctgcg cgttggcgac ccctggggtt cgttgcacta ccgggacgag cgagtcgggg
     2821 accggggggc gggtggagtt ggcgttgcgg ccctgggcct tgaacggctc tcggcctcgc
     2881 accacggcgt cgacaccctt cgtcgtggtc gtgaagaggt tgtcgaagac gctgccgagg
     2941 gtgtcggtca gcacgaaggt gcccgacatg aaggcgacgc cgagcaccac cgccagtgac
     3001 gtcaggacga gacgaagctt cttggcgaac aggttcttga gcgtgacgtg gcccatcccg
     3061 ggccgctcag tcgccgatgc gcttcatgcg gtccaggacc gagtcggcgg ttgggccggc
     3121 catctcgtcg acgatgcgtc cgtcggcgag gaagacgacc cggtcggcgt acccggcgcc
     3181 gatgggatcg tgggtcacca tgacgatcgt ctggccgagg tcgtcgacgg cttgacgcat
     3241 gaacgagagg atctccgcgc cagcacgcga gtcgaggttg ccggtcggct cgtcggcgaa
     3301 gatcacgttc ggccggctga cgagggcccg ggcgaccgcg acgcgctgct gttggccgcc
     3361 ggacagctcc gtcggacggt gcgacatccg gtcgcgcaga ccgacgatgt cgatgacatg
     3421 gtcgagccac gccgggtcgg gtttccggcc cgcgagggcc atcgggagcg tgatgttctc
     3481 gagcgcgttc aaggtgggga tcaggttgta ggactggaag acgaagccga tgttgtcgcg
     3541 tcggatgagg gtcagctgct tctcggacgc cg
//
LOCUS       DATCCH010000430         2475 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_450143, whole genome shotgun sequence.
ACCESSION   DATCCH010000430 DATCCH010000000
VERSION     DATCCH010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450143"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="VIJ44_08275"
     CDS             complement(<1..816)
                     /locus_tag="VIJ44_08275"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEY5165932.1"
                     /translation="MNVDLVQLAGSRGRLSSTRVDPVTAEVVRGAMETVCFEMATYVS
                     RTATTPILNQSNERNATILDARGRLAALSVGIPQFMLTSTLPVRFALEFLGVDEFNEG
                     DVFVANDPYHGGGHLPDYNVFAPVFADDGTGDRRMVLIASIQCHHGDTGGGVPGGYNV
                     TAGDIWGEGVRWPVVKVLDRGVERRDVLYALQTNNRIPDYIGDLRAQIGAAQLGVQRL
                     GNILDRFGVPGVEGSVDVMIDYAASRFREEVTSWPDGVYEADAYVDHDPLGHPD"
     gene            918..2435
                     /locus_tag="VIJ44_08280"
     CDS             918..2435
                     /locus_tag="VIJ44_08280"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY5165933.1"
                     /translation="MESPRLGISAHAAATPNKAALISGDVTRTFAEFEDRTNRLAHAL
                     WVRGVRAGDRVAVMLPNGIEFFEAWAAATKLDAPVVLVNWHLKADELAYILGDSKARV
                     LIAHRRLAAQYEDALAGVDGCDVVVVPDEYEAIIGRHTEAPARAIDALDAPIFYTSGT
                     TGRPKGVVHGRPDSGRARQNMQGQLALWGWTEGDTYVVTGPAYHAGPGGWAMAALYVG
                     ATTVILESFDARTWLRLVEAHQVTRTFMVPAHFIRILEVPETERRALDLSSLQLIAHA
                     GAPCPVPVKHRIIEALAPCEIAELYGMSEGGATRIAMAEWLERPGSVGRPWPGTEVRI
                     LDSDNGEVLAPGATGIIYVRPPGGARFHYHDDVTKTADAWRDDAFTVGDVGHLDEDGY
                     LYLTDRAVDMVIRGGVNIYPREIEDVLYTHPTVVDCAVFGVPHDQLGEELMAVVETRT
                     PTTPDELREFCRDHLADFKVPAHIELVDELPRQPNGKVLKRVLRDEHWAGRVSRI"
BASE COUNT          390 a          852 c          857 g          376 t
ORIGIN      
        1 gtcgggatgg cccagcgggt cgtgatcgac gtacgcgtcg gcctcgtaca cgccgtcggg
       61 ccagctggtg acctcttcgc ggaaccgact ggcggcgtag tcgatcatca cgtcgacgct
      121 gccttcgacc ccggggacac cgaagcggtc gaggatgttc ccgagccgct gcaccccgag
      181 ctgcgcagcc ccgatctggg cgcgcagatc accgatgtaa tccgggatcc ggttgttggt
      241 ctgcagcgcg tacaagacgt cgcggcgctc gacgccacgg tcgaggacct tgacgaccgg
      301 ccagcggacc ccttcacccc agatatcgcc cgcggtcacg ttgtacccgc cgggcacgcc
      361 gcccccggtg tcgccgtgat ggcactggat gctggcgatc agcaccatcc gccggtcccc
      421 ggttccgtca tcggcgaaga ccggtgcgaa cacgttgtag tcggggaggt gaccgccgcc
      481 gtggtacggg tcgttggcga cgaacacgtc accctcgttg aactcgtcga cgccgaggaa
      541 ctcgagcgcg aaccgcaccg ggagcgtcga cgtgagcatg aactgcggga tgccgaccga
      601 cagcgcagcc agacgcccgc gggcgtcaag gatggtggcg ttgcgctcgt tgctctggtt
      661 caagatcggc gtcgtcgcgg tgcgcgacac gtaggtcgcc atctcgaagc agaccgtctc
      721 catcgccccg cggacgacct cggcggtgac cgggtcgacc cgcgtgctgc tgagtcggcc
      781 ccggctgccg gcaagctgca ccagatcgac gttcatgatc ggctcctccc gggctccccc
      841 cggaacctga cgcccatgtc acgtctatcc taaacccgac gtcgtcggag acggttgccc
      901 gcacgccagg gagggccgtg gagtcgcctc ggctcgggat cagcgcccac gcggcggcga
      961 cgccgaacaa ggccgcgctc atcagcggcg acgtgacgcg gacgttcgcc gagttcgagg
     1021 accggaccaa ccgacttgcg cacgccctgt gggtgcgagg cgtgcgggcc ggggaccgtg
     1081 tggcggtgat gctccccaac ggcatcgagt tcttcgaggc gtgggccgcg gcgacgaagc
     1141 tcgacgcccc ggtggtgctc gtgaactggc acctgaaggc cgacgagctc gcgtacatct
     1201 tgggggactc gaaggcccgg gtgctcattg cgcaccgccg gctcgcggcc cagtacgagg
     1261 acgccctcgc cggagtcgac ggctgtgacg tcgtcgtggt ccccgacgaa tacgaggcca
     1321 tcatcggtcg gcacacagag gccccggcga gggccatcga cgccctcgac gcgccgatct
     1381 tctacacgtc cggcacgacc ggccggccca agggcgtcgt tcacgggcgg ccggattcgg
     1441 gtcgggcccg tcagaacatg caaggccagc tggcgctgtg gggctggacc gagggcgaca
     1501 cgtatgtcgt gaccggcccg gcgtatcacg ccggcccggg cggctgggcg atggcggcgc
     1561 tgtacgtcgg cgcgacgacg gtcatcctcg agtcgttcga cgcccggacg tggctccgtc
     1621 tcgtcgaggc gcaccaggtg acacgcacgt tcatggtccc ggcgcacttc atccgcatcc
     1681 tcgaggtgcc agagaccgaa cgtcgcgccc tcgacttgtc atcgctgcag ctgatcgccc
     1741 atgccggtgc gccgtgcccg gttccggtga agcaccggat catcgaggcg ttggcgccgt
     1801 gcgagatcgc cgagctgtac gggatgagcg agggtggcgc gacccgaatc gcgatggccg
     1861 agtggctcga acggccgggc agcgtcggcc ggccttggcc cgggaccgag gtccgaatcc
     1921 tcgactccga caacggcgag gtgctggccc ccggagcgac agggatcatc tatgtgcggc
     1981 caccgggtgg ggcccggttc cactaccacg acgacgtcac caagacggct gacgcctggc
     2041 gcgacgacgc gttcaccgtc ggcgacgtgg gtcatctcga cgaggacggg tacctgtacc
     2101 tcaccgaccg ggcggtcgac atggtcattc gaggaggggt caacatctac ccgcgggaga
     2161 tcgaggacgt cttgtacacc catcccacgg ttgtcgactg cgcggtgttc ggtgtgcccc
     2221 acgaccagct cggtgaggag ctcatggcgg tcgtcgagac ccgcacccca acgacacccg
     2281 acgagctccg cgagttctgt cgggaccatc tcgccgactt caaggtgccg gcccacatcg
     2341 agctcgtcga cgagctcccc cgccaaccta acggcaaggt cctcaagcgc gtcctccgag
     2401 acgagcattg ggccgggcgg gtgtcccgga tctgagctcg gcgcgggtca gtcgaggagc
     2461 agctccgcga gccgg
//
LOCUS       DATCCH010000431         4134 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_617394, whole genome shotgun sequence.
ACCESSION   DATCCH010000431 DATCCH010000000
VERSION     DATCCH010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4134
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617394"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="VIJ44_08285"
     CDS             complement(<1..497)
                     /locus_tag="VIJ44_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018564244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEY5165934.1"
                     /translation="MTGAASVWVDGALVDLAAARVSPLDHGLLVGDGVFETLAVYGGV
                     PFAWRRHHDRLRRSAAGLGLTVPPGAELRAAAEAVLQANGIRDGRLRVTVTGGPSPLG
                     SERGDAAPTVIVAGAPTRQRPPVDDVVTVPWPRNERGATAGLKTISYAENVRALAYAQ
                     QRGAS"
     gene            650..2077
                     /locus_tag="VIJ44_08290"
     CDS             650..2077
                     /locus_tag="VIJ44_08290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEY5165935.1"
                     /translation="MIALAVAAAVMIGSIIAVNYVIDTKLNSVKRVTVKTAAAPSQGA
                     NYLLLGSDTRAFVKTKQQQNAFGTPGTVNSDTMMVIHVEPGAKKTLIVSFPRDLWVNI
                     PGMGMNKINAAFGVGPDKAIQTLKADFNIDINHFVSVDFQSFQAVVNAIGSVPVYFPY
                     PARDLETGLNVSGGCLSLNGQTALAYTRSRTLQFYSSVQKQWLLADPTADIGRINRQQ
                     QFIRELAGIAVQRSLEDPLTANTIVDRVLQYLVLDQSLSKTDIFTLVDAFRSVNPNDT
                     SHLEFQTLPWATGPDQNGLSVLYVKAPDDQGVLAQLRDFSGKATTTPTVTITPQNVKV
                     RFLDGGGASASASSALFDLVRRGGFQSGGVGTVSSPVAQTEVRYRPGAFTQGALVLEY
                     LQPAARLVADPTIKGADVAVVLGRDFRSIVLPAGPAGVGAGASAGSASAGTPSTGDTT
                     AQTSSGTSVDPARFGPPIPKGPPCK"
     gene            2100..3380
                     /locus_tag="VIJ44_08295"
     CDS             2100..3380
                     /locus_tag="VIJ44_08295"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009944678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEY5165936.1"
                     /translation="MGVVGAGYVGLTTGACFAHLGHDVVVADIDADKVAQLEKGDIGI
                     LEEGLPELVSEGLATRRLRFVVGAADAAADAEFVFLCVPTPRGSDGDADLSVVEAVVR
                     EVAPLLRPGTVVVNKSTMPVGSTTLVARQLANAGAVTELGVASNPEFLREGTAARDFL
                     HPYRVVIGADDPAVAVRVSELYRDVQAPVLVTNPASAEMIKYASNAFLAMKVSYVNAI
                     ANLCEAVTADVREVTLGMGYDPRIGFEFLHPGPGWGGSCFPKDTAALLHTAARVGDSF
                     PLLASVISTNEEQRDRVVSKVRDALDRTDGRRVGVWGLTFKANTNDLRDSPAIAVARH
                     LVADGMSVRAYDPMGGESATDLVPGLEVVTDPYEACADAEVLVVLTEWDEFRWFDFTR
                     VAGLMAARSIVDARNLLDPAAMRHLGFAYAGIGR"
     gene            3380..>4134
                     /locus_tag="VIJ44_08300"
     CDS             3380..>4134
                     /locus_tag="VIJ44_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007630010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY5165937.1"
                     /translation="MARAVVTGGAGFIGSHLCTALRARGEEVVAVDNLSTGERDNLTH
                     LLGDPGFAFLERDVIDGLTVDGPVDAVLHFASAASVPEYLARPLETLRVGAEGTRHAL
                     ELARDRGARFLVASTSEIYGDPLVHPQPEGYWGNVNPVGPRSVYDEAKRYGEALTMAY
                     HRTHGVDTKIVRIFNCFGPGLLPGDGRVVSNFLVQAMAGEPLTVYGDGTQTRSFCFID
                     DEVRGILALLDSRWNGPMNIGNPDEHTVLELAKL"
BASE COUNT          628 a         1470 c         1381 g          655 t
ORIGIN      
        1 tccgacgccc cccgttgctg tgcgtacgcc agggcacgaa cgttctcggc ataggagatg
       61 gtcttgagac cggcggtggc gccgcgttcg ttgcgcggcc acggcacggt cacgacatcg
      121 tcgaccggcg gccgctgccg agtgggggcg ccggcgacga tgaccgtcgg ggccgcgtcg
      181 ccgcgctccg aacccagcgg ggacggcccc ccggtgacgg tgacgcgcaa gcggccgtcg
      241 cggatcccgt tcgcctgcag gaccgcctcc gcggcggcgc gcagctccgc accgggtggc
      301 accgtcagcc cgagtcccgc cgccgatcgg cgaaggcggt cgtggtgtcg ccgccacgcg
      361 aacgggaccc ctccgtagac ggcgagggtc tcgaacaccc cgtcgccgac gaggagaccg
      421 tgatcgagtg gcgacacccg cgccgctgcg aggtcgacga gcgcgccgtc gacccacacc
      481 gaggctgcac cggtcatggc tgctcggccc gaccgctgcg ctgctggggt tggcacttca
      541 cgggcttgag ggtacgcgcc ccgcggggag ccgccgcgac cccgtcggta cactgagcgc
      601 tcccatgggg ctctctcggt cggtggttcg cgcagtctca aagcggtacg tgatcgcttt
      661 ggccgtcgcg gccgcggtga tgatcggcag catcatcgcg gtcaactacg tcatcgacac
      721 gaagctcaac agcgtgaagc gggtgacggt caagaccgcc gccgccccgt cgcagggcgc
      781 gaactacctg ctcctcggct ccgatacgcg agccttcgtg aagacgaagc aacagcagaa
      841 tgcgttcggc acgcccggaa ccgtcaactc ggacaccatg atggtcatcc acgtcgagcc
      901 gggtgcgaag aagacgctga tcgtgtcgtt cccacgcgac ctctgggtca acattcccgg
      961 gatggggatg aacaagatca acgccgcgtt cggcgtcggt cccgacaagg ccatccagac
     1021 cctcaaggcc gacttcaaca tcgacatcaa tcacttcgtc tcggttgact ttcagagttt
     1081 ccaagccgtc gtcaacgcga tcgggtccgt acccgtctac ttcccgtacc cggcccgcga
     1141 cctcgagacc ggcctgaacg tctccggggg ctgcctgtcc ctcaacggtc agaccgcgtt
     1201 ggcgtacacc cggtcccgga cgctgcagtt ctacagctcg gtgcagaagc agtggctgct
     1261 cgccgacccg acggcggaca tcggtcgcat caaccgtcag cagcagttca ttcgggagct
     1321 ggccggcatc gcggtgcagc gcagcctcga ggacccactc accgccaaca ccatcgtcga
     1381 ccgggtgttg cagtacctgg tgctggacca gagtctctcg aagactgaca tcttcaccct
     1441 cgtcgacgcg ttccggagcg tgaatccgaa cgacaccagt cacctcgagt tccagaccct
     1501 gccctgggcg accggccccg accagaatgg tttgtcggtg ctgtacgtga aggcacccga
     1561 cgatcagggc gtgctggccc aactgcgcga cttcagcggc aaggccacca cgacgccgac
     1621 ggtgacgatc accccccaaa acgtgaaggt gcggttcctc gacggtggcg gtgcgtccgc
     1681 gtcggcgtcg agcgcgttgt tcgacctcgt gcgccgcggc gggttccagt ccggcggcgt
     1741 cgggaccgtc tcgtcgccgg tggcccagac cgaggtgcgc taccggcccg gggcgttcac
     1801 ccagggcgcc ttggtgttgg agtacctcca gcccgcggct cgactcgtgg cggacccgac
     1861 gatcaagggc gccgacgtcg cagtcgtgtt gggtcgcgac ttccggtcga tcgtcctccc
     1921 ggccggaccg gctggcgtgg gcgccggggc gtcggcgggc tccgcgtcgg ccggcacccc
     1981 ttcgaccggt gacaccaccg cccagacgtc gagcgggaca tcggtcgacc cggctcggtt
     2041 cggaccgccg atcccgaagg gtccgccgtg caagtgacgg cgccgcggcc gtgagtcgag
     2101 tgggcgttgt cggggccggg tacgtcgggc tcaccaccgg cgcctgcttc gcgcatctgg
     2161 gacatgacgt ggtcgtcgcc gacatcgacg ccgacaaggt ggcccagctc gagaagggcg
     2221 acatcggcat cctcgaggaa ggcctccccg agctggtgtc cgaaggtctc gccacgcgcc
     2281 gcttgcggtt cgtcgtcggc gcggccgatg cggccgcgga cgccgagttc gtgttcctct
     2341 gcgtcccgac cccgagaggg tccgacggcg acgccgacct ctcggtcgtc gaggcggtcg
     2401 tgcgcgaggt cgcgccactg ctgcgccccg ggacggtcgt ggtcaacaag tcgacgatgc
     2461 cggtcggctc gacgacgctc gtcgcccgac agctcgcgaa cgcgggcgcg gtcaccgagc
     2521 tcggtgtggc atcgaaccct gagttcctcc gggaggggac tgccgcccgc gacttcctcc
     2581 atccgtaccg ggtcgtcatc ggtgccgacg atcccgcggt agcggtccga gtgtcggagc
     2641 tgtaccgcga cgtgcaagcg ccggtcctgg tcaccaaccc ggcgtcggcc gagatgatca
     2701 agtacgcctc gaacgcattt ctcgccatga aggtgtcgta cgtcaacgcg atcgcgaatc
     2761 tgtgtgaggc agtcaccgcc gacgtccgcg aggtgaccct cggcatgggc tacgaccctc
     2821 ggatcgggtt cgagtttctc catcccgggc ccggctgggg cggctcgtgc ttccccaagg
     2881 acaccgcggc cctcctccac accgcggctc gggtcgggga cagcttcccg ctcttggctt
     2941 cggtgatctc gaccaacgag gaacagcgcg accgggttgt gagcaaggtc cgagacgcac
     3001 tcgaccggac cgacggccgg cgggtcgggg tctggggcct caccttcaag gccaacacca
     3061 acgatctacg agattcgcct gcgatcgcag tcgcccggca cctcgttgcc gacgggatgt
     3121 cggtgcgggc ctacgacccc atgggcggcg agtccgcgac cgacctggtc ccagggctcg
     3181 aggtcgtcac cgacccgtac gaggcgtgcg ccgacgccga ggtgctcgtg gtgctgaccg
     3241 aatgggacga gttccggtgg ttcgacttca cccgcgtggc cggcctcatg gccgcccggt
     3301 ccatcgtcga cgcccgcaac ctgctcgacc ccgcggcgat gcgccacctc gggttcgcgt
     3361 acgcgggtat cggtcgctga tggcgcgggc cgtcgtcacc ggcggcgccg ggttcatcgg
     3421 ctcacatctc tgcaccgcgc tccgggcccg aggcgaggaa gtggtcgccg tcgacaacct
     3481 ctccaccggc gaacgcgaca acctcaccca tctgctcggc gaccctggct tcgccttcct
     3541 cgagcgggac gtcatcgacg gtctcaccgt cgacggcccg gtcgacgcgg tgctgcactt
     3601 cgcgagcgcc gcgagtgtcc ccgagtacct ggcgcgcccc ctcgagacgc tccgggtcgg
     3661 tgccgaaggg acccgccacg cgctcgagct cgctcgcgac cgcggtgcac ggttcctcgt
     3721 ggcctcgacg agcgagatct acggcgaccc gctcgtccat ccgcaacccg agggctactg
     3781 gggcaacgtc aacccggtcg gtccccgatc ggtctacgac gaagcgaaac ggtacggcga
     3841 agcgctgacg atggcgtacc accgcaccca tggtgtcgac acgaagatcg ttcgcatctt
     3901 caactgcttc ggcccggggt tactgcccgg cgacgggcgc gtggtctcga acttcctcgt
     3961 ccaagccatg gccggtgaac cactcacggt ttacggcgac gggacccaga cccgctcgtt
     4021 ctgcttcatt gacgacgagg tccgggggat cctcgcgctg ctcgactcgc gctggaacgg
     4081 tccgatgaac atcggcaatc cggacgagca caccgtgctc gagctggcca agct
//
LOCUS       DATCCH010000432         3558 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_617937, whole genome shotgun sequence.
ACCESSION   DATCCH010000432 DATCCH010000000
VERSION     DATCCH010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617937"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..1188)
                     /locus_tag="VIJ44_08305"
     CDS             complement(121..1188)
                     /locus_tag="VIJ44_08305"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY5165938.1"
                     /translation="MRPAPLPVDVDDVSPSWLSEVLDVPVTEVEVLARHSGTTGRARI
                     ALTYGHDAPDRPASMFVKLAPFDERQRRFVDAVGLGIAEARFYRDVAASVAVRVPRVW
                     HAAVENGRYVMVLEDLVSSGCRFPSPDDRDLTAVTGRLVDELARLHAPFWGSPALDDG
                     WLAFVGDGGRLAFGAGGPFIARAVAQFAAEMPAVFSRLGELYIEAAPRVAELWAAPPR
                     TLAHGDPHLGNLFVDGERPGFFDWAMVMPRAGMWDVAYVLCGSVPTELRRAHEAEWIR
                     RYCDGLARRGVALDEAEAWDQYRLYAIYAWVSSTSTAGVGDRWQTETVGRGGMARATA
                     AVDDLDAVAHLEARLAGTVSS"
     gene            1423..1716
                     /locus_tag="VIJ44_08310"
     CDS             1423..1716
                     /locus_tag="VIJ44_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165939.1"
                     /translation="MARIRILDPTATPPELTPEPGPDAGSLAGRLVGLRTDETWQSWE
                     WVIDEWKQSLDAAGASIRVWNAGNRIGDEGERTFQELDEFASSVDVAIVGLGN"
     gene            1991..3082
                     /locus_tag="VIJ44_08315"
     CDS             1991..3082
                     /locus_tag="VIJ44_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011601660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165940.1"
                     /translation="MTDSNLASRVDCGPDGCTIDWLASARLEVDDDPVAFTKLATDAG
                     WGDGLPLIPPTEERVRAFVAASGRFPDECLCDLPPRNGRATVEKVAINAVMAGAPADA
                     MPLLCSATEALADPIFNLFALNTTTSCVVPGLFVNGPSRDRLGIPYDVGCFGGAAGPG
                     PAIGRATRLMMRNVGGQVVGVSSKSVFGSPGRVSGIVVGEWEERSPWSPLAERRGVVG
                     DAVTVHGCTGTIDVADIVADNGVDLLELIGKSLAFPGTNAFIGAHHGAEILVCLAPPW
                     ADLIAAAYPDLTDVQERLHADAALPVSWWPAPHQAKAHEAGRVDDAGLVHLVEGPDHM
                     LVMVCGGLGNLHALALHSFGPTRAVTRPF"
     gene            3084..>3558
                     /locus_tag="VIJ44_08320"
     CDS             3084..>3558
                     /locus_tag="VIJ44_08320"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5165941.1"
                     /translation="MALRVLNMLGDRAAAAARDVVPDAEIITVPNDAIPDDLRGEVLF
                     AAWSLGHPVFDRLDDLGVRWMHLPGTGIDAWPRTLLEGRTVTCARGVSAIPIAEFVLG
                     AILAFEKRFPDIWLDTPPKHWNVADLSELADKTVGLVGLGGIGSAVAHRVRAFDAR"
BASE COUNT          524 a         1320 c         1206 g          508 t
ORIGIN      
        1 tcgacgccct cgactaccgc gggcgactct cggtcgagta cttcgacctg ccggagtacg
       61 gctggcccct cgacgatccg ctcgcctggg cgcgggccct cgccgagcac gtccgagccc
      121 tcatgagctg accgtcccgg cgaggcgcgc ctcgagatgc gccaccgcgt cgaggtcgtc
      181 gaccgcggcg gtggcgcggg ccatcccgcc ccgcccgacc gtctcggtct gccagcggtc
      241 cccgacgccc gcggtcgagg tcgacgacac ccaggcgtag atcgcgtaga ggcggtactg
      301 gtcccacgct tcggcttcgt cgagcgcgac gccccggcgg gcgagcccgt cgcagtagcg
      361 gcggatccac tccgcctcat gagcgcgtcg cagctcggtg gggaccgagc cgcacaagac
      421 gtaggccacg tcccacatgc cggcccgagg catcaccatg gcccagtcga agaacccggg
      481 ccgctcgcca tcgacgaaca ggttcccgag gtgggggtcc ccgtgcgcga gcgtccgcgg
      541 cggtgccgcc cacagctcgg cgacccgtgg agccgcctcg atgtacagct cgcccaaccg
      601 actgaacacc gcgggcatct cggccgcgaa ctgcgccacc gcgcgggcga tgaacgggcc
      661 cccggcaccg aacgcgagcc gcccaccgtc acccacgaac gccagccatc cgtcgtcgag
      721 cgccggcgac ccccagaacg gggcgtgcaa ccgggcgagc tcgtcgacga gacgtccggt
      781 caccgcggtg aggtcacgat cgtcggggct cggaaaccga cagccgctcg agacgaggtc
      841 ctcgagcacc atcacgtagc ggccgttctc gaccgcggca tgccacaccc gagggacccg
      901 caccgcgacg ctcgccgcga cgtcccgata gaagcgggcc tccgcgatcc cgagcccgac
      961 cgcgtcgacg aaccgtcgct ggcgctcgtc gaagggggcg agcttcacga acatcgacgc
     1021 cggccggtcg ggggcgtcgt ggccgtaggt gagcgcgatg cgggcccgtc cggtggtgcc
     1081 cgagtggcgc gcgagcacct cgacctcggt cacgggaacg tcgaggacct ccgacagcca
     1141 cgatgggctg acgtcgtcga cgtcgacggg caggggcgcg ggcctcacct caccgcgacc
     1201 gtggcgtcgt gcccggtcgc gtccggaacc gtcggcaccg gcccggtgcg gtgcgacccg
     1261 aaggagccga tgctggcatc tcgccctttc tcctgaccgc ttcggccagt ctcgcacagc
     1321 cggctcgccg caccggtggc cgccagccca agacgatcga ttccgcagcg gaccgcggtc
     1381 ggtacgatcg gctgtgccgg ccccagagag aagagaggtg cgatggcccg catcaggatc
     1441 ctggatccca ccgcgacacc acctgagctc acccccgaac cgggacctga cgccgggtca
     1501 ctcgccgggc gcctcgtcgg tttacgcacc gacgagacgt ggcagtcgtg ggagtgggtg
     1561 atcgacgagt ggaagcagtc cctcgacgcg gccggtgctt cgatccgggt ctggaacgct
     1621 ggcaaccgaa tcggcgacga aggcgagcgg accttccagg agctcgatga gttcgccagc
     1681 agtgtcgacg tcgcgatcgt cgggctcggc aactgaggct cctgcacctc gtggagcgtt
     1741 cacgacaccg tcgcggcagc tctcgaggac aagacggcgg tcgtcgtcgt aaccgcagag
     1801 ttcgaatccc tcgcgcacac catggccgcc aatgccggcc gggcgggtct gcgcgtccat
     1861 gtcctcccgt acccactgga aacgcgagcc gaggccgacg ttcgggccat cgcgcgtcag
     1921 cactggccag ccttgctcca aacgatcggg gcctcgccgt gacggcgccc gctcctgctc
     1981 ggtcggcgcg gtgacggaca gcaacctcgc gtcacgggtc gactgcggac cggacgggtg
     2041 cacgatcgac tggctggcca gcgctcgcct cgaggttgac gacgatccgg tggcgttcac
     2101 caagctcgcg accgacgccg ggtggggtga cggtctgccg ctcatcccgc caaccgagga
     2161 acgagtgcga gccttcgtcg ccgcctcggg gcggttcccc gatgaatgtc tctgcgacct
     2221 gcccccgcgc aacgggcgcg ccaccgtgga gaaggtggcg atcaacgcgg tgatggccgg
     2281 cgcacctgcc gatgccatgc cgctgctgtg ctcggcgacc gaagcgctgg ccgaccccat
     2341 cttcaacctg ttcgcgctca acaccacgac ctcctgcgtt gtcccgggcc tcttcgtgaa
     2401 cggtccgagc cgagaccggc tcgggatccc ctatgacgtc ggctgcttcg gcggggctgc
     2461 gggtcccgga cccgcgatcg gtcgcgccac tcgcctcatg atgcgcaacg tcggcggtca
     2521 ggtcgtcggt gtgtcctcga agagcgtctt cgggagcccg ggtcgcgtca gcgggatcgt
     2581 ggtcggcgaa tgggaggaac gctcgccgtg gtcaccactc gccgagcggc gcggcgtcgt
     2641 cggggacgcg gtcacggtgc atgggtgcac cgggacgatc gacgtcgccg acatcgtcgc
     2701 cgacaacggc gtcgatctcc tcgagctgat cggcaaatcc ctcgcgttcc ctggcaccaa
     2761 cgcgttcatc ggcgcgcatc acggcgccga gatcctcgtc tgtcttgctc cgccgtgggc
     2821 cgacctcatc gcggccgcgt acccggacct caccgacgtc caggaacgac tccacgccga
     2881 cgcggcgctg cccgtctcgt ggtggccggc tccgcaccag gcgaaggccc acgaggccgg
     2941 ccgcgtcgac gacgccgggc tcgtgcacct cgtcgagggc cccgaccaca tgctggtcat
     3001 ggtctgcgga gggctcggga acctgcatgc gctggcgctg cacagcttcg ggccgacgcg
     3061 cgccgtcacc cgaccgttct gacgtggcgc tgcgcgtact gaacatgctc ggcgatcggg
     3121 ccgccgcggc ggcacgcgac gtcgtccccg acgccgagat catcaccgtt ccgaacgacg
     3181 ccatccccga cgacctccgc ggggaagtgc tgttcgcggc ctggtcactc ggccatcccg
     3241 tctttgaccg gctcgacgac ctcggtgtgc gttggatgca ccttcccggc accgggatcg
     3301 acgcctggcc gcgcacgctg ctcgaggggc gcacggtcac ctgcgcccgc ggcgtcagcg
     3361 cgatccccat cgccgaattc gtgctcggcg ccatcctggc gttcgagaaa cggttccccg
     3421 acatctggct cgacacgccc cccaaacatt ggaacgtcgc cgacctcagt gagctcgccg
     3481 acaagactgt cgggctcgtc gggctcggcg ggatcggatc cgcggtcgcg caccgcgttc
     3541 gcgccttcga cgctcggg
//
LOCUS       DATCCH010000433         2568 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_70321, whole genome shotgun sequence.
ACCESSION   DATCCH010000433 DATCCH010000000
VERSION     DATCCH010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70321"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1771)
                     /gene="ppdK"
                     /locus_tag="VIJ44_08325"
     CDS             complement(<1..1771)
                     /gene="ppdK"
                     /locus_tag="VIJ44_08325"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013555502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEY5165942.1"
                     /translation="MQYVYAFEEGSKEQKYLLGGKGANLAEMTNLGLPVPPGFTITTE
                     ACNAYMSHDHVLPDGLLDEVATRLADLETKMGKTLGADSDPLLVSVRSGAPFSMPGMM
                     DTVLNLGLNDTSVTGLAKQTNNERFAYDSYRRFVQMFGKIVLDIPGEHFETALEALRD
                     ARGVATDPELSADDLAELVETFKSIVKDATQHEFPQEPHEQLRYAIEAVFKSWDGRRA
                     RDYRKLEGIPDDLGTAVNVQTMVFGNKGDDSGTGVAFTRDPATGEPRAYGDFLRNAQG
                     EDVVAGIRVTEPLDAMANHFSDCHHQLLDTMQRLEHHYRDMCDIEFTIEQGRLFMLQT
                     RVGKRTAAAALRMAVDMQAEGLIDTREAVLRIQPAQLDQLLHPQFDPKATYTPLTKGL
                     NASPGAAVGTVCFTADDAEARRENGERVILVRPETSPDDLHGMIAAEGILTSRGGLVS
                     HAAVVARGMGKPAVCGAAALDIDVNAGQLAVGDTIVRLGDTISINGTTGEVVLGAVPV
                     ITPEPTGPFETILAWADEFRRLGVRTNADLPNDAIVARRFGAEGIGLCRTEHMFLGDR
                     LPIVQRYILADSEAEEAAALTELE"
     gene            complement(1834..>2568)
                     /locus_tag="VIJ44_08330"
     CDS             complement(1834..>2568)
                     /locus_tag="VIJ44_08330"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEY5165943.1"
                     /translation="VIQAARDEEGRLRAAWQVLSDPVQRQRYDDLAGLVPSADGDHHD
                     LAPDHDHDDDQDHDQDHDDDEGEDDSVDSAVPATRDRSRRNPPSMITAEGLEIPSAGR
                     RATAAGIDTITLLVLYVVIVYPIIFGLGLTGIPAVIAGIGTPVFLIVVYLIIPTYRGG
                     QTLGKRYTYTMVVDRATGQLPTLSQVVRRYTIPAFAVVLLQQTGGLIALMYAVSFLMN
                     RDQVSLADRMAKTAVIIARYRPDRLA"
BASE COUNT          347 a          676 c         1066 g          479 t
ORIGIN      
        1 gctcgagctc ggtgagggcg gcggcctctt cggcttcgct atcggcgagg atgtaacgct
       61 gcacgatggg gagtcggtcg ccgagaaaca tgtgctcggt gcggcagaga ccgatccctt
      121 cggcgccgaa gcggcgggcg acgatggcat cgttggggag gtcggcgttg gtgcgcacgc
      181 cgaggcgtcg gaactcgtcg gcccaggcca ggatggtctc gaacggcccg gtgggttcgg
      241 gggtgatgac cggcaccgcg ccgaggacga cttcgccggt ggtgccgttg atggagatcg
      301 tgtcgccgag gcggacgatg gtgtcgccga cggccagttg gccggcgttc acgtcgatgt
      361 cgagggcggc ggcaccgcac accgcgggtt tgcccatgcc tcgggcgacg accgcggcgt
      421 ggctgacgag gccgccccgg gaggtgagga tgccttcggc cgcgatcatg ccgtggaggt
      481 cgtcgggtga ggtctcgggg cggaccagga tgacgcgttc accgttctcg cgtcgggctt
      541 cggcgtcgtc ggcggtgaag cagacggtgc cgaccgcggc gccgggtgag gcgttcagcc
      601 ctttggtgag cggggtgtag gtggccttcg ggtcgaactg gggatggagc agctggtcga
      661 gttgggcggg ctggatgcgc agaaccgctt cgcgggtgtc gatgagacct tccgcttgca
      721 tgtcgacggc catgcggagc gcggcggcgg cggtgcgttt cccgactcgg gtctggagca
      781 tgaagagtcg accctgttcg atggtgaact cgatgtcgca catgtcgcgg tagtggtgtt
      841 cgagtcgctg catggtgtcg agcagctggt ggtggcagtc ggagaaatgg ttggccatgg
      901 cgtcgagggg ttcggtgacg cggatgccgg cgacgacgtc ttcgccttgg gcgttgcgga
      961 ggaagtcgcc gtacgcgcgg ggctcgccgg tggcggggtc gcgggtgaac gcgacaccgg
     1021 tgccggagtc gtcgcctttg ttgccgaaga ccatggtctg gacgttgacg gcggtgccga
     1081 ggtcgtcggg gatgccttcg agcttgcggt agtcgcgggc gcggcgtccg tcccaggatt
     1141 tgaagaccgc ttcgatggcg tagcggagct gctcgtgggg ttcttggggg aactcgtgtt
     1201 gggtggcgtc cttgacgatg cttttgaagg tttcaacgag ctcggcgagg tcatcggcgc
     1261 tcagctcggg gtcggtggcg acaccgcggg cgtcgcggag tgcttcgagg gcggtttcga
     1321 agtgctcgcc ggggatgtcg aggacgatct tgccgaacat ctgcacgaag cggcggtagg
     1381 agtcgtacgc gaagcgctcg ttgttggttt gtttggcgag gccggtgacc gaggtgtcgt
     1441 tgaggccgag gttcaagacg gtgtccatca tcccgggcat ggagaacggt gcgccggagc
     1501 ggaccgagac gaggaggggg tcgctatccg cgccgagggt tttgcccatc ttggtttcga
     1561 ggtcggcgag tcgggtggcg acctcgtcga ggagcccgtc ggggaggacg tggtcgtggc
     1621 tcatgtaggc gttgcaggct tcggtggtga tggtaaagcc gggcggtacg gggagaccga
     1681 ggttggtcat ctcggcgagg ttggcgccct tgccgccgag caggtacttc tgctccttgg
     1741 agccctcctc gaacgcgtag acgtactgca cgaaccctcc tgggacgtcg atgatctgag
     1801 cctagtgacg gcgccgcggg ggtcggtcgg ggttcaggcc aggcggtcgg gtcggtagcg
     1861 ggcgatgatg accgcggtct tggccatacg gtcggcaagg gagacctggt cgcggttcat
     1921 gaggaacgag acggcgtaca tgagcgcgat gagtccgccc gtctgctgga ggagcacgac
     1981 ggcgaaggcc gggatggtgt agcggcggac gacttggctg agggtgggga gctgtcccgt
     2041 ggctcggtcg acgaccatcg tgtacgtgta gcgcttcccg agggtctggc cgccccggta
     2101 ggtggggatg atgaggtaga cgacgatcag gaatacgggg gtgccgatcc cggcgatgac
     2161 ggcggggatg ccggtgaggc cgaggccgaa gatgatcggg tagacgatca cgacgtacaa
     2221 gacgagcagg gtgatggtgt cgatgccggc ggcggtggcg cgccggcccg cggaagggat
     2281 ctcgaggcct tcggcggtga tcatcgacgg cgggttgcgt cgggaccggt cgcgggtggc
     2341 gggcacggcg ctgtcgacgg agtcgtcctc gccctcgtcg tcgtcgtggt cttggtcgtg
     2401 gtcttggtcg tcgtcgtggt cgtggtcggg ggcgaggtcg tggtggtcgc cgtccgcgga
     2461 cggcacgagt ccggccaggt cgtcgtagcg ctggcgttgg acggggtcgc tgaggacctg
     2521 ccaggcggcc cggaggcggc cttcctcgtc gcgggcggct tggatgac
//
LOCUS       DATCCH010000434         3552 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_243594, whole genome shotgun sequence.
ACCESSION   DATCCH010000434 DATCCH010000000
VERSION     DATCCH010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243594"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="VIJ44_08335"
     CDS             complement(<1..598)
                     /locus_tag="VIJ44_08335"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HEY5165944.1"
                     /translation="MDRGRLEALSDGVFAVALTLLALNLTVAGPGHGPLGHQLTHQWP
                     AYVAYAISFFTIGIIWVNHHALFRNFAAVDRVLVFSNLLLLFFVVAIPFATATMAAYL
                     RGGGADARLATAVYAGVFEGMSLAFGLVFVWPIYRRLLIAPLDPASARRATIRFTIGN
                     LAYVVAIAVAFLSPAAALAISALVAVYYVFERTPAPPRG"
     gene            complement(649..960)
                     /locus_tag="VIJ44_08340"
     CDS             complement(649..960)
                     /locus_tag="VIJ44_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase accessory protein"
                     /protein_id="HEY5165945.1"
                     /translation="MSDVLADDLTRLTGATAPPRDNGALAFQAPWEGRALALAVALVE
                     RLEIPWDDFRACLIASIADQPDRPYYESWAAALEQLVLDHELTTREALDAATPIERPV
                     L"
     gene            complement(957..1520)
                     /gene="nthA"
                     /locus_tag="VIJ44_08345"
     CDS             complement(957..1520)
                     /gene="nthA"
                     /locus_tag="VIJ44_08345"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HEY5165946.1"
                     /translation="MAPVEARVEALEAVLAEQGLLDAAAVDAIVSHFEEDLSPLNGAK
                     VVARAWVDDGYKARLLAHGTAAIAELGFAGPEGDHMVVVANTAAVHNLVVCTLCSCYP
                     WPILGLPPAWYKTPAYRSRAVREPRALLEEMGTVIPDDVEIRVWDSSAEVRYLVLPER
                     PVATDQLDEAELADRVTRDSMIGVERL"
     gene            complement(1550..2197)
                     /gene="nthB"
                     /locus_tag="VIJ44_08350"
     CDS             complement(1550..2197)
                     /gene="nthB"
                     /locus_tag="VIJ44_08350"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit beta"
                     /protein_id="HEY5165947.1"
                     /translation="MDGVHDLGGIAGFGAVEIEADEPMFHQDWERRVFRLTTAANMTG
                     TVSSGGAFRHSIERMEPAHYLNSGYYEHWLTGIATLVVERGLVTRAELDELAGGRFPL
                     SRVDRGIAPDLGADDQTVPRFEIGREVRVRAWHPAGHTRAPRYVQGKRGVVVRHDGAF
                     SVPDVEAHSDRRRLEPTYSVRFTAGELWGEGGAPGEVVHVDLWESYLEDDRTERR"
     gene            complement(2746..>3552)
                     /locus_tag="VIJ44_08355"
     CDS             complement(2746..>3552)
                     /locus_tag="VIJ44_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165948.1"
                     /translation="VTVATVLVAALLGLGGLGAYVARYRRRDPVFPKRRSISVLALYV
                     SVYSVVVIGAGKIAGASVDQRIVTPGYVPALLLGAVLFEVTIQRLARIGERRRVAIAP
                     GLVVLVALAYFASTAVSFTSQTWRNGEIARGYTQKNSMGFELVRSVETLAPGVLVATN
                     RPWTLFAATGREPIVPSPGVVAPELALTPILESQLASQACTRPVYLAWFTYAAQWPYT
                     PAQLAGHVDLHPLQVLSDGVLYSVRPKGADPDCSEPVASKRGATVDGDAF"
BASE COUNT          625 a         1189 c         1209 g          529 t
ORIGIN      
        1 caccgcgcgg cggggccggg gtgcgttcga acacgtagta gaccgcgaca agcgcgctga
       61 tcgccagcgc cgccgccggg gagagaaacg cgaccgcgat ggcgacaaca tacgcgaggt
      121 tcccgatcgt gaaccggatc gtcgcccggc gcgccgatgc cggatccagg ggagcgatca
      181 gaagccggcg atagatgggc cacacgaaca cgagaccaaa ggccagggac atcccttcga
      241 agacacccgc gtataccgcg gtggcgagac gcgcatccgc acccccaccg cgcagatatg
      301 ccgccatcgt cgcagttgcg aacggaatcg ccacgacgaa gaacagcaac aacaagttcg
      361 agaacacgag cacgcgatcg acggcggcga agttacggaa caaggcgtga tggttcaccc
      421 agatgatccc gatcgtgaag aagctgatcg cgtaggccac gtaggccggc cactggtgcg
      481 tgagctggtg tccgagcggg ccatgtcccg gccccgcgac ggtgagattg agggcgagca
      541 gcgtcaacgc gaccgcgaag accccgtcgc tcagcgcctc gagtctcccg cgatccatct
      601 cgtcctttga ccctgtcgag cttgttcggc ctaccgcgcg gcacccgcct acagcacggg
      661 ccgctcgatc ggcgtcgcgg cgtcgagcgc ctcgcgagtc gtgagctcat gatcgagtac
      721 gagctgctcg agcgccgcgg cccaactctc gtagtacggc cggtcgggct ggtccgcgat
      781 gctggcgatg aggcacgccc ggaaatcgtc ccaggggatc tcgaggcgct cgacgagggc
      841 gaccgccaac gccaaggccc gaccttccca cggcgcctgg aatgccaacg ccccgttgtc
      901 gcgtggcggg gcggtggcgc ccgtcaggcg ggtcaggtcg tcggcgagaa cgtcgctcac
      961 agtcgttcga ccccaatcat cgaatcgcgc gtcacgcgat cggcgagttc ggcttcgtcg
     1021 agctggtcag ttgcgacggg tcgttctggg agcacgaggt agcgcacctc ggcgctggag
     1081 tcccagactc gaatctccac gtcatcgggg atcacggttc ccatctcctc gaggagcgca
     1141 cgcggctcgc gcaccgcccg cgagcggtag gcgggggtct tgtaccaagc cggcgggagg
     1201 ccgaggatgg gccacggata gcacgagcac agggtgcaga cgaccaggtt gtgaaccgca
     1261 gccgtgttcg cgacgacgac catgtggtcg ccctcgggtc cagcgaaccc cagctcggcg
     1321 atcgccgcgg tcccgtgagc gagcagccga gccttgtacc cgtcgtcaac ccatgcccgg
     1381 gcgacgacct tcgcgccgtt cagcgggctg agatcctcct cgaagtggga cacaatcgcg
     1441 tctaccgccg cggcgtcgag gagcccctgc tcggccaaga ccgcctcgag cgcctcaacc
     1501 cgtgcctcga ccggggccat tggctctgga tggtgggcgt ggtcgtagct caccggcgct
     1561 ccgtgcgatc gtcttcgagg tagctctccc agaggtcgac gtggaccact tcgccaggcg
     1621 cgccaccttc tccccacagc tccccggcgg tgaagcgaac cgagtaggtc ggctcaagtc
     1681 gccgtcggtc actatgcgcc tcgacatcgg ggaccgagaa cgcaccgtcg tgtcggacca
     1741 cgacaccgcg cttgccctga acgtaacgcg gtgccctcgt gtggccggcc gggtgccaag
     1801 cccgcacccg tacctcgcgg ccgatctcaa agcggggaac ggtctggtcg tcggcgccta
     1861 ggtcgggagc gattccacgg tcgacccggg agagcgggaa ccgaccgccg gcgagttcgt
     1921 cgagctcggc gcgggttacc aggccgcgct cgacgacgag ggtggcgatg cccgtcaacc
     1981 agtgctcgta gtagccggaa ttgagatagt gggcaggctc catccgctcg atgctgtgac
     2041 ggaaggcccc tccgctgctg acggttccgg tcatgttggc ggcggtggtc aggcgaaaga
     2101 cgcgccgctc ccagtcctga tggaacatcg gctcatcggc ttcgatctcg accgcgccga
     2161 agccggcgat gccgccgagg tcgtggacac cgtccatgcg tccagtctcg cacgaccccc
     2221 gcccgccggt ctcgggctca ccgagtcact ccaggtgccg gccgagccga cgagcagcga
     2281 gaccgcggtc tagccgatga cgaacccgaa gacacccggg aggccgccag acggagctcc
     2341 cgacggcctc cggtcccgcg acgcagaccg gctgccggag ggtcggtgct cgtgccgaca
     2401 aacaccggca acgaccgggt cgtgatctcg ttcgcggtcc cggcaacgac cgcggcgatc
     2461 atcgcgtcgg ctcctccatt ggctgggcgt cggtgcccgc caggccgtca cccgcggacc
     2521 gccaaccgtc atcgaggtcc caatgtcggg cgactcgcgg ttcagtacgc cgcgagtcga
     2581 agcctcggca cggtcccgat cggagccggt gacaactcac ggcttgttgg acacgagtcg
     2641 gggttgtgca cccaccgatg cgttccgcgg agcgtggtca ggtatccggc cgtgacgccg
     2701 gtggtgtctg aagcaggcgc ggtcagcggc ggaggttgtc gcgagctaga acgcatcacc
     2761 gtcgacggtc gcaccacgct tcgaagcgac gggctcggag cagtcgggat cggcaccctt
     2821 gggccggacc gaatagagca ctccgtcgct cagtacctgg agcgggtgga gatcgacgtg
     2881 accggcgagc tgcgcggggg tgtagggcca ctgggccgcg tacgtgaacc aggctaggta
     2941 gacggggcgg gtgcaggctt ggctggcgag ctgcgattcg aggataggtg tgagggccag
     3001 ttccggagcc accactcccg ggctggggac gatgggttct cgacccgtcg ccgcgaagag
     3061 cgtccacgga cggttggtgg ccaccaacac tcctggagcc aacgtttcga cgctgcgcac
     3121 aagctcgaaa cccatcgagt tcttctgtgt gtatccccgt gcgatctcgc cgtttctcca
     3181 ggtttgagac gtgaaggaaa ctgcggtcga cgcgaaatat gcaagcgcga ccaagacgac
     3241 gagcccgggc gcgatggcga cccgtcgtcg ttcgccaatg cgcgccagtc tctggattgt
     3301 gacttcgaag agcaccgcgc ccaggagcag cgccgggacg taacccgggg tcacgatgcg
     3361 ttgatcgacg ctcgctcccg cgatcttgcc ggccccgatc acgacgactg aatagaccga
     3421 cacatagagg gccaggacag aaatggagcg tcgttttggg aacaccggat cgcggcgccg
     3481 gtaccgggcg acataggcgc cgagtcctcc aaggccgagc aacgcggcta ccagtaccgt
     3541 cgcgaccgtc ac
//
LOCUS       DATCCH010000435         3786 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_451643, whole genome shotgun sequence.
ACCESSION   DATCCH010000435 DATCCH010000000
VERSION     DATCCH010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451643"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1210
                     /locus_tag="VIJ44_08360"
     CDS             <1..1210
                     /locus_tag="VIJ44_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020528486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEY5165949.1"
                     /translation="AFKDTVNFWRTWIGRSQYAGRWRESVNRSALVLKLLVSQTHGSL
                     VAAPTFGLPEKIGGERNWDYRYTWIRDASFTLFALIRLGYTEEAGAFMRWIHDRCMDL
                     NPDGSLQIMYGHDGRKTLTEQTLPHLEGYRGSSPVRIGNGAYDQLQLDIYGELMDAVY
                     LYNKHGQPIPYELWTNLVRLVDWVCDHWQLPDEGVWEVRGGRQEFLYSRLMCWVALDR
                     GIRLADRRSFPYPFEKWRSVRDAIYHEMMTIFWDERRRTFVQYPGVDTVDASTLLMPL
                     VKFVSPTDPRFLDTLKAIDADLVDDSLVYRYKIGEAAQDGLMGDEGTFNMCSFWFAEA
                     VARSGDVQRGRFFFEKMLGYANHLGLYGEELGPRGEHLGNFPQAFTHLGLISAAHSID
                     RKLSHAGWVG"
     gene            1263..2885
                     /locus_tag="VIJ44_08365"
     CDS             1263..2885
                     /locus_tag="VIJ44_08365"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5165950.1"
                     /translation="MGTGRRSLIGALGVALVAGSLGGIAGSAGAATGPKHGGSIIYSV
                     EAETPGYCLPGGSQLAPAGIEVASAIYDTLTTINTKNQYVPYLAQSVTPNATLDQWTI
                     TLRPNIKFHNGEALDANAVKLNLDTYRGLNPKIPSPLSSFTFKNVASVDVTGPLTVVV
                     TTKTPWAAFPAYLYGSGRTGIVAPAQLADNNTCSTNLIGTGPFKLALGGYRLNESTTV
                     VRNPHYWRPGLPYLDSIKFVPAQEAAQQLNGLQAGDLDVIQTRDAHNIIQLRQRKASG
                     QISEQDTDNGTDVGYGMLNVGAAPFNDMNARLALAYAGDANQINQIRNQGLETLATGP
                     FAPATPYYLTFAQAGLPTHNVAKAKAYARLYSQAHGGQTLSYTYLTPGADPELLALAE
                     LTKQQDAQAGINVNIQQVDQATGISNAIAHQFQFLAWANHPQADPDTQYAWWYSGSPV
                     NFGQINDPVINQLLDQGRVESNPAKRIQIYRQLNKQFATKLYNLWAFYTKWSVGAQKN
                     IGGLNGAPLPDGHGQAYALFEGIVPTVTLYKK"
     gene            complement(2914..>3786)
                     /locus_tag="VIJ44_08370"
     CDS             complement(2914..>3786)
                     /locus_tag="VIJ44_08370"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5165951.1"
                     /translation="PAPMSGATVVVTGANAGVGLETAAGLVALGARVVVTARDEAKGA
                     RAADRIRARGGPGTVEVAPLDLASFASIRRFAREVGPGLGRLDVLVNNAGLVQLDHTV
                     TEDGFETTFGVNHLGHFLLTTLLLDQLRSSAPARVVVVASLAHRSARSGLDFDDLQAE
                     RGYSPIGAYNRSKLANIYFTRELARRLVGSGVTANSLHPGFVASRLGRDGDGGVLADI
                     AMTLGRPFAISPARGARTSVYLASSPAVADVTGRYFVRCREAHPARVATDDDAAQRLW
                     SVSEELVSSVPDPG"
BASE COUNT          675 a         1404 c         1169 g          538 t
ORIGIN      
        1 cgccttcaaa gacaccgtca acttctggcg cacgtggatc ggccgctccc agtacgcggg
       61 ccgctggcgc gagagcgtca accgctccgc gctggtcctc aaactgctgg tctcccaaac
      121 ccacgggtca ctggtcgcgg cgccgacctt cgggctcccc gagaagatcg gcggcgaacg
      181 caactgggac taccgctaca cctggatccg cgacgcctcg ttcaccctgt tcgcgttgat
      241 ccggctcggc tacaccgagg aggccggggc gttcatgcgc tggatccacg accgctgcat
      301 ggacctgaac ccggacgggt cgctgcagat catgtacggc cacgacggcc gcaagaccct
      361 caccgagcaa accctcccgc acctcgaggg ctaccgcggg tcgtcgccgg tccggatcgg
      421 aaacggcgcc tacgaccagc tccagctcga catctacggc gagctgatgg acgccgtcta
      481 cctctacaac aagcacgggc agcccatccc ctacgagctg tggacgaacc tggtccgcct
      541 cgtcgactgg gtctgtgacc actggcagct ccccgacgaa ggcgtctggg aggtccgcgg
      601 cggacgccaa gagttcctgt actcgcggct catgtgctgg gtggccctcg accggggcat
      661 ccgtctcgcc gaccgacggt cgttcccgta cccgttcgag aaatggcgga gcgtccgcga
      721 cgccatctac cacgagatga tgacgatctt ctgggacgag cggcgccgga cgttcgtcca
      781 gtaccccggc gtcgacaccg tcgacgcctc gacgctgctc atgccgctgg tcaagttcgt
      841 cagccccacc gatcctcgct tcctcgacac cctcaaggcc atcgacgccg acctcgtcga
      901 cgattccctg gtctaccggt acaagatcgg cgaagccgcc caggacgggc tgatgggtga
      961 cgaggggacg ttcaacatgt gctcgttctg gttcgccgag gccgtcgccc gctccgggga
     1021 cgtccaacgg ggccggttct tcttcgagaa gatgctcggc tacgccaacc acctcgggct
     1081 ctacggcgaa gagctcggac cccgcggcga gcacctcggc aacttccccc aggccttcac
     1141 ccatctcggc ctcatcagcg ccgcccactc gatcgaccgc aagctctccc acgccggctg
     1201 ggtcggctga ccgcggggac ccggcgccgc ggcctacgat gcgcccggtc aagggggaca
     1261 tcatgggcac aggacgtcgg tcacttatcg gcgcgttggg cgtggcgctc gtcgcggggt
     1321 cgctcggcgg gatcgcgggg tccgcgggag cggccacggg cccgaagcac ggcggctcga
     1381 tcatctactc cgtcgaggcc gagaccccgg gctactgcct cccgggcggt tcgcagctgg
     1441 caccggcggg catcgaggtc gcctccgcga tctacgacac cctcacgacc atcaacacca
     1501 agaatcagta cgtcccgtac ctcgcgcagt cggtgacgcc gaatgccacc ttggaccagt
     1561 ggacgatcac gcttcggccc aacatcaagt ttcacaacgg tgaggcgctc gacgccaacg
     1621 cggtgaagct caacctcgac acctaccggg gactgaaccc caagatcccg tcgccattga
     1681 gcagcttcac gttcaagaac gtggcgagcg tcgacgtcac cggtccactc accgtggtcg
     1741 tcacgaccaa gaccccgtgg gcggccttcc ccgcctacct ctacggcagc ggacggaccg
     1801 gaattgtcgc cccggcgcag ctcgccgaca acaacacctg ctcgacgaac ctgatcggga
     1861 ccggaccgtt caagctggcg ctcggggggt accggctgaa cgagtcgacc acggtcgtga
     1921 ggaatccgca ctactggcgt ccgggactcc cgtacctcga ctcaatcaag ttcgtgcccg
     1981 cccaggaggc ggcgcagcag ctcaacggcc tccaggccgg cgacctcgac gtgatccaga
     2041 cccgcgacgc ccacaacatc attcagctgc gccaacgcaa ggcgagcggc cagatctccg
     2101 agcaggacac cgacaacggc accgatgtcg gctacgggat gctcaacgtc ggggcggcgc
     2161 cgttcaacga catgaacgcc cggctcgccc tcgcgtacgc cggcgacgcg aaccagatca
     2221 accagatccg caaccagggc ctcgagacgc tcgcgacggg tcccttcgcc ccggccacgc
     2281 cctactacct gacgtttgcc caggccgggc tgcccaccca caacgtggcg aaggccaagg
     2341 cgtacgcccg cctgtactcc caagcccacg gaggccagac cctctcctac acctacctga
     2401 cccccggcgc cgaccccgag ctgctcgcgc tcgccgagct caccaagcaa caggacgcgc
     2461 aggcgggaat caacgtcaac atccagcagg tcgaccaagc aacgggaatc agcaacgcga
     2521 tcgcgcacca attccagttc ctggcgtggg ccaaccaccc ccaggctgac cccgacacgc
     2581 agtacgcctg gtggtacagc ggatcgccgg tcaacttcgg tcagatcaac gaccccgtga
     2641 tcaaccagct cctcgatcag ggccgggtcg aatcgaaccc cgcgaaacgc attcagatct
     2701 accggcagct gaacaagcag ttcgcgacga agctctacaa cctatgggcc ttttacacga
     2761 aatggtcggt cggcgcccag aagaacatcg gcggcttgaa cggcgcgccg ctccccgatg
     2821 gccacggaca ggcctacgcc ctgttcgagg gcatcgtccc gaccgtcacg ctgtacaaga
     2881 agtaaccgag acgagctgag ctggtcgcgt ccgtcacccg gggtcgggga cggacgagac
     2941 cagctcctcg ctcaccgacc agagacgctg cgccgcgtcg tcgtcggtcg cgacgcgagc
     3001 ggggtgtgcc tcgcggcagc gtacgaagta ccgcccggtg acgtccgcga ccgcggggct
     3061 cgacgccagg tagaccgacg tccgcgcccc ccgggcgggg ctgatcgcga acggtcgacc
     3121 gagcgtcatg gcgatgtcgg cgaggacccc gccatcaccg tcccggccga gccggctggc
     3181 gacgaaaccc ggatgcaacg agttcgcggt cacgccgctt cccaccaggc gccgggccag
     3241 ctcccgggtg aagtagatgt tcgccagctt cgaccggttg tacgccccga tcgggctgta
     3301 cccccgctcg gcttggaggt cgtcgaagtc caggccactc cgggccgacc ggtgcgcgag
     3361 cgacgcgacg accacgaccc tcgccggggc gctggaccgc agctggtcga gcagcagcgt
     3421 cgtgaggagg aaatggccga gatggttcac cccgaacgtc gtctcgaagc cgtcctcggt
     3481 cacggtgtgg tccagctgca ccagcccggc gttgttcacc aacacgtcga gtcggccgag
     3541 acccggcccg acctcccggg caaagcggcg gatcgacgcg aagctcgcca gatccagcgg
     3601 ggcgacctcc accgtgccgg ggccgccccg agcccggatc cggtccgcgg ccctcgcccc
     3661 ttttgcctcg tcccgggcgg tgaccaccac ccgggcgccg agggcgacca ggcccgccgc
     3721 agtctcgagg ccgacgccgg cgttggcgcc ggtgaccacc accgtcgcgc cactcatcgg
     3781 agccgg
//
LOCUS       DATCCH010000436         3028 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_451896, whole genome shotgun sequence.
ACCESSION   DATCCH010000436 DATCCH010000000
VERSION     DATCCH010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451896"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="VIJ44_08375"
     CDS             complement(<1..624)
                     /locus_tag="VIJ44_08375"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY5165952.1"
                     /translation="MHIEQSWTRLAVILAVAIGAGMGAVTGAGSAAHATSVTKPSSSA
                     VTTTSQTLVDTTRQRTLETMFFSPRRSTGPAPLLVFVHGYDQSPSDYRNLLKAWASAG
                     YVVAAPKFPSTNRDAASLDASDYHNEPMDVSFVISQVIAQSAASTGPLSGRVDATRVG
                     VAGHSLGAEVVLGLLNQCCRDPRVRAAISLAGSEQFNPGEPAFPPSGY"
     gene            complement(859..1536)
                     /locus_tag="VIJ44_08380"
     CDS             complement(859..1536)
                     /locus_tag="VIJ44_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165953.1"
                     /translation="MFGRDLARRWLALVLLVALPVVFYLTSRASEGGHAFTAGGLGLS
                     WAIAGASLFTALGARGVDQRLVFDGYRPVELLGARLLLLDAAGVVLSALFAALLIVVS
                     GTPDVSSLVLALVLVALVAVPLGLVLAVLVPHELEATLLLIGLIGIEMILPSASSTVG
                     DLPLGGPQELLDRAAGKAHPVAVATAVGRSALWIVALTVIGAVIWAWRTRVDRPATPD
                     PLPTAED"
     gene            complement(1569..2192)
                     /locus_tag="VIJ44_08385"
     CDS             complement(1569..2192)
                     /locus_tag="VIJ44_08385"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5165954.1"
                     /translation="MSRSPASALRVRGVSKRFGHRVVLNTVDLDVRPGEAVALVGENG
                     AGKTTLLRICAGLIRADGGTVTVDGRIGYCPQQATFLESLRVDEHLILFGRAHGLGRA
                     DARRRGQMLLAGLEFSADPGQTIRELSGGARQKLNLTLALLGDPQVLLLDEPYQGFDR
                     GSYISFWDQIARWCAAGRGVLVVTHLLAELDRVDRIVGLPTTTSSSR"
     gene            complement(2242..>3028)
                     /locus_tag="VIJ44_08390"
     CDS             complement(2242..>3028)
                     /locus_tag="VIJ44_08390"
                     /inference="COORDINATES: protein motif:HMM:NF020135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HEY5165955.1"
                     /translation="VDHVSAVTFRPDDVVLVATKSQQTADALAELTDAAPPDTAVICA
                     QNGVENERLALRRFSNVYGVCVMCPASHLEPGVVRAHRAPVPGILDLGRYPSGVDATA
                     AELAAELTSAGFLSDPKADIMRWKYKKLLLNLGNAIEAICGPETRGGALYERAHREGV
                     ACLDAAGIAYVTDADDAARRGDLIPRPPGGEPRGGSSWQSMTRQTGNIEADYLNGEIV
                     LLGRLHGVPTPVNAVLQRLANQLASARTAPGSMTADDILALIS"
BASE COUNT          487 a          977 c         1144 g          420 t
ORIGIN      
        1 gtacccgctg ggtgggaacg ccggctcgcc cgggttgaac tgctccgaac ccgcgagcga
       61 gatcgcggcc cgaacccggg gatcgcgaca gcactggttg agcagtccca acacgacctc
      121 ggcgccgagg gagtgacccg cgacaccaac tcgcgtcgcg tcgactcgac ccgagagcgg
      181 cccggttgag gccgcgctct gggcgatgac ctgggagatg acgaagctca cgtccatcgg
      241 ctcgttgtgg tagtcgctcg cgtcgaggct ggctgcgtcg cggttcgtcg acgggaactt
      301 cggtgcggcg acgacgtagc ccgccgaagc ccaggctttg aggaggttgc gatagtcgga
      361 cggggactgg tcgtagccgt ggacgaagac gaggagcggc gccgggcccg ttgaccgacg
      421 aggcgagaag aacattgtct cgagggtgcg ttgacgggtc gtgtcgacca gggtctggct
      481 ggtggtcgtc accgcggagc tcgacggttt cgtcaccgac gtcgcatggg ctgcgctccc
      541 cgcgccggtg accgccccca tgccggctcc gatcgcgacc gcgaggatca ccgccagccg
      601 cgtccaggac tgctcgatgt gcaccgacgg acggtaacgc cccccggccg tcaggatcac
      661 gagacgctcg atgaggacgg tggtcggcga gctgggctcg gctcgccgcg ccgggggagc
      721 acaacgacga gccgagccac agccgtggcg cccgcatatg agggtctcgc cgtcgcgggc
      781 tgctcggact cgagcactac gaaacggagc gtctcgggga ttgccccgtg ctgcggcgct
      841 tcgaaccagc tcggccggtc agtcctcggc ggtggggagc ggatctgggg tcgcgggacg
      901 gtcgacgcgg gtgcgccagg cccagatcac cgccccgatg acggtgaggg cgacgatcca
      961 caacgccgag cggcccaccg cggtcgcgac ggcgaccggg tgcgccttcc cggcggcgcg
     1021 gtcgaggagc tcctgggggc cgccgagggg aaggtcaccg accgtgctgc tcgccgacgg
     1081 gaggatcatc tcgatgccga tcaggccgat gagcaacaag gtggcttcga gctcgtgggg
     1141 gaccagcacc gcgaggacga ggccgagggg gaccgcgacc aacgcgacga gcacgagtgc
     1201 gaggacgagg ctgctcacgt ccggggtgcc cgagacgacg atgagcagcg cggcgaagag
     1261 ggcgctgagc acgaccccgg ccgcgtcgag gaggaggagg cgcgcgccga gcagctcgac
     1321 gggccggtag ccgtcgaaga cgaggcgctg gtcgacgccc cgggcgccga gcgcggtgaa
     1381 gaggctggcg ccggcgatcg cccagctcaa cccgagtccg ccggcggtga acgcgtggcc
     1441 gccctcggat gcgcggctcg tgagatagaa gaccaccggg agggcgacga ggagaacgag
     1501 cgccagccag cggcgggcaa ggtcgcgtcc gaacatctca gccgcggtga gggtacgggc
     1561 gaacccgttc atcgcgacga cgacgtcgtc gttgggagcc cgacgatgcg gtcgacgcga
     1621 tccagctcgg cgagcagatg ggtgacgacc agcacgccgc gtcccgcggc gcaccatcgc
     1681 gcgatctggt cccagaagct gatgtagctg ccgcgatcga agccctggta cggctcgtcg
     1741 aggaggagga cctggggatc gccgagcaag gccagggtga ggttcagctt ctggcgggcg
     1801 ccaccggaga gctcccggat ggtctgtccc gggtcggccg agaactcgag accggccagg
     1861 agcatctggc cgcggcggcg cgcgtcggcc cgaccgaggc cgtgggcgcg gccgaacagg
     1921 atgagatgct cgtcgacgcg cagcgactcg aggaacgtcg cctgttgggg gcagtagccg
     1981 attcgtccgt cgaccgtcac cgtgccgccg tcggctctga tcagtccggc gcagatgcga
     2041 agcaaggttg tcttgccggc gccgttctca ccgacgaggg cgacggcttc gccgggccgc
     2101 acgtcgagat cgacagtgtt gagaaccacg cggtgcccga atcgcttcga gacccctcgc
     2161 acccgcagcg cggaggccgg cgagcggctc accggcggtg gcgtatcgag cgagctgatc
     2221 gacgggtatg ggcgatgtcg ttcacgagat cagggccaag atgtcgtccg cggtcatcga
     2281 gccgggcgcg gtgcgagccg aggcgagctg gttggcgagc cgctgcaaga ccgcgttgac
     2341 cggtgtcggc acgccgtgga gtcgcccgag caggacgatt tcgccgttca ggtagtcggc
     2401 ttcgatgttg ccggtctggc gggtcatgct ctgccacgac gagccaccgc gtggctcgcc
     2461 tcccggggga cgagggatga ggtcgccgcg ccgggcggcg tcgtcggcgt cggtcacgta
     2521 cgcgatgccg gccgcatcga ggcacgcgac accttcacgg tgcgcgcgtt catagagcgc
     2581 tccgcctcgg gtctcgggcc cgcagatcgc ctcgatggcg ttgccgaggt tcaacaacag
     2641 cttcttgtac ttccagcgca tgatgtccgc cttcgggtcg gacaagaagc cagccgacgt
     2701 caactcggcg gccagctcgg ccgcggtggc gtccaccccg gatgggtacc ggccgaggtc
     2761 gaggattccc ggaaccgggg cccggtgggc ccggaccaca ccaggttcga gatggctcgc
     2821 cggacacatg acacagaccc catacacgtt cgagaatcgg cgcagggcga gtcgttcgtt
     2881 ctcgaccccg ttctgggcac agatcaccgc ggtgtccggc ggtgccgcgt cggtcaactc
     2941 ggcgagcgcg tcagcggttt gctggctctt ggtggccacg aggacgacat cgtcgggtcg
     3001 gaaggtgact gcagagacgt ggtcgacg
//
LOCUS       DATCCH010000437         3131 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_362066, whole genome shotgun sequence.
ACCESSION   DATCCH010000437 DATCCH010000000
VERSION     DATCCH010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362066"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            266..865
                     /locus_tag="VIJ44_08395"
     CDS             266..865
                     /locus_tag="VIJ44_08395"
                     /inference="COORDINATES: protein motif:HMM:NF015553.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium resistance transporter"
                     /protein_id="HEY5165956.1"
                     /translation="MVATVLLAVGLYATTNIDNLVVLTAFYSDPSYRSGQVTAGEYLG
                     LAMIVAVSAGGAAAASAVPHRYIGLLGLVPLTLGAVKLAALLRGARPPDPDTTVGAGR
                     HRPFVVSAVTVGNGGDNIAAYVPIFATRSFGEAAVIVVVFFVAAGVWCAAARAIAHAG
                     WTSRAMNRFSPIVVPVVYVALGLSILTFSGTLGWLARAG"
     gene            complement(885..1394)
                     /locus_tag="VIJ44_08400"
     CDS             complement(885..1394)
                     /locus_tag="VIJ44_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165957.1"
                     /translation="MRPGRARWRRAAMMVGAAVGVVTFGPAASSGAASSPAPTACALL
                     SAGEVQTALGGSVADGSLTAAPDGTQSVCDWVVTLSSSRGYGVQLDVYGGRTAADFAL
                     QRKIARGRTGTIKHLGARAFSERAVVADQVFDDLWVQHATINFRVEILKDLGSKPLEP
                     LARLVLSRL"
     gene            1509..2033
                     /locus_tag="VIJ44_08405"
     CDS             1509..2033
                     /locus_tag="VIJ44_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017303893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEY5165958.1"
                     /translation="MIERDAARRHPKQLPARRRPTRRASRRRVTLATVIALGALLSAC
                     SSSSVTTVGGCAISHGASCEGTYMKGADIVRGNLYGANLSRADLRQAVLVGAILTGAN
                     LTNTNLQGADLRHAHLEDANLNRADLRGARFDRAWLAGATFVGARGVSLATLESGIVC
                     RTVTASGVVLNRDC"
     gene            complement(2017..3042)
                     /locus_tag="VIJ44_08410"
     CDS             complement(2017..3042)
                     /locus_tag="VIJ44_08410"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY5165959.1"
                     /translation="MTADPATFGVQPDVDAPGSEPRRRWRPTRLQWALGIWLVAVGVM
                     LWKWHLTTDRTHLFIIIGTGLIAAGAGRLRTVARVVIDWAPLFFILAAYDSLRGHADK
                     WFPVNVTPQIHADQFLFGGTVPTVELQHALFTPGHPHVWDYAAFFVYLTHFFAALFVA
                     ALLWKFAYPKFRRFAALFVTLSFAAFATYALFPAAPPWLASQNHALAPTAKIVDEIWT
                     HLGLQNGASLLSARSHEANPVAAIPSLHAAYTMLLMLFFWRSAGRWRWLLPLYPLAMG
                     FSLVYTGEHYVIDILLGWLYAVVVYFVGTWLMDRWARRRSRVSVEAPEPEPPPPVLAG
                     AALSSRD"
BASE COUNT          469 a         1114 c         1087 g          461 t
ORIGIN      
        1 gcccgcgacg acgggttggc ccgtcgggcc ggtcccgccg atcacgagcg ctcgtcggct
       61 cacggcccct cgatttccgt ccggaacgcc ggtgcccgat gagcgtcccg cacacgacct
      121 gttctagtgg tcggagccgg cccgcgtcgt cggcgtcgcg gcgggccgat gtccaccggt
      181 gcgtgctccg ccatggccga gcccgtggcc tcggcggcgg ctagcgtccg cgtccgcgcg
      241 tgccgcgcga actgctcgga gcttcgtggt cgccaccgtc ctcctcgccg tcgggttgta
      301 cgccacgacc aacatcgaca acttggtcgt cctgaccgcg ttctactccg acccgtcgta
      361 ccggtcgggg caggtcaccg cgggtgagta cctcgggttg gcgatgattg tggcggtcag
      421 cgccggtgga gccgccgcgg cgtcggccgt gccccaccgc tatatcggcc tgctcggcct
      481 cgtcccgttg acactcggtg cggtcaagct cgccgccctc ctccgcggcg cccgtccgcc
      541 cgaccccgac accacggtcg gcgccgggcg ccaccgcccc ttcgtcgtca gcgcggtcac
      601 cgtcggcaac ggtggcgaca acatcgccgc gtacgtcccg atcttcgcca cccggtcgtt
      661 cggcgaggcc gccgtgatcg tcgtcgtctt cttcgtggcc gcaggggtct ggtgcgccgc
      721 cgcccgggcc atcgcccatg ccggctggac cagccgggcc atgaaccgct tcagccccat
      781 cgtcgtcccg gtcgtgtacg tcgcgctcgg cctgtcgatc ctcacgttct ccggaacgct
      841 cgggtggctc gcccgagccg gatgacgcct cggcccgggc ggcgtcagag cctggagagc
      901 acgagacggg cgagcggttc cagtggcttc gaccccagat ccttgaggat ctccactcgg
      961 aagttgatcg tggcgtgctg gacccagaga tcgtcgaaga cctggtcggc gacgacggcc
     1021 cgttcgctga aggcgcgggc accgaggtgc ttgatcgtcc ccgtacgtcc ccgggcgatc
     1081 ttgcgttgca aggcgaaatc ggccgcggtt cgccccccgt agacgtcgag ctgcacgcca
     1141 tatccccgag acgagctcaa ggtgacgacc cagtcgcaca cgctctgggt cccgtcgggt
     1201 gccgcggtga ggctcccgtc ggcgacgctg ccacccaacg cggtctgcac ctcgcctgcg
     1261 ctcaggaggg cgcaggcggt cggcgccggg ctcgacgcgg cccctgacga cgccgcaggc
     1321 ccgaacgtga ccacgccgac ggcggcaccg accatcatcg ccgctcgccg ccaccgagcg
     1381 cgtcctggtc gcatcctcgc gcacggtagc ggcggctccg cctccggctc ggcgtccccc
     1441 gagcagctag gtcatcagcg tcgaggggcg atccccttat gggccgcgga cgccactacg
     1501 ttcggccgat gatcgagcgc gatgccgccc ggcgacaccc caagcagctg ccagcccgac
     1561 gccgacccac tcggcgggcg tctcggcgac gcgtgaccct ggccacggtg atcgcgctcg
     1621 gtgcgttgct ctcggcctgc tcgtccagct cggtcaccac cgtgggcggt tgtgccatct
     1681 cgcacggcgc gagttgtgag ggcacctaca tgaagggggc cgacatcgtt cggggcaacc
     1741 tgtacggagc gaacctcagc cgcgctgatc tccgccaagc tgtcctcgtc ggcgccatcc
     1801 tcaccggagc gaacctgacc aacacgaacc tccagggagc cgatctccgc cacgcccacc
     1861 tcgaggacgc gaacctgaac cgcgccgact tgcgcggggc tcgcttcgac cgggcctggc
     1921 tcgcgggtgc gacgttcgtg ggcgcgcgcg gcgtgagcct cgcgaccctc gagtcgggca
     1981 tcgtgtgccg gacggtcacg gcgtccggcg tcgtcctcaa tcgcgactgc tgagcgcggc
     2041 cccggcgagt accggcggtg gcggctccgg ctcaggcgcc tcgaccgaga cccgagatcg
     2101 gcgacgagcc cagcggtcca tgagccaggt gcccacgaag tacacgacga ccgcgtagag
     2161 ccaacccaag aggatgtcga tgacgtagtg ctcaccggtg tacacgaggc tgaatcccat
     2221 cgcgagggga tacagcggaa gcagccaccg ccaccgtccg gccgaccgcc agaagaacag
     2281 catgagcagc atcgtgtagg cggcgtggag cgacgggatc gccgcgaccg ggttggcctc
     2341 gtggctgcgg gccgagagga ggctggcgcc gttctggagg ccgaggtggg tccagatctc
     2401 atcgacgatc ttcgccgtcg gggccagggc atggttctgg ctggccagcc acggtggcgc
     2461 ggcgggaaac agggcgtagg tcgcgaacgc cgcgaagctc aaggtgacga atagggccgc
     2521 gaaacggcga aacttcgggt aggcaaactt ccacagcaac gccgcgacga acaacgcggc
     2581 gaagaagtgc gtcaggtaga cgaagaacgc ggcatagtcc cagacgtgag ggtgcccggg
     2641 cgtgaagagc gcgtgctgga gctccaccgt cgggacggtg ccgccaaaga ggaactggtc
     2701 ggcgtggatc tgaggggtga cgttgaccgg gaaccacttg tcggcgtgac cccgaagcga
     2761 gtcgtaggcc gcgaggatga agaacagcgg ggcccagtcg atcacgaccc gggccacggt
     2821 ccgcagccgt ccggctccgg ccgcgatgag gccggtcccg atgatgatga acaagtgggt
     2881 tcggtcggtg gtcagatgcc acttccacag catcacgccg acggcgacga gccagatccc
     2941 gagcgcccac tgcagccggg tgggccgcca acgtcgtcgg ggttcggagc caggcgcgtc
     3001 gacgtcgggc tgcaccccga aggtggccgg gtcagccgtc atccgccgtc catcgtctgt
     3061 tccccggcgc gcaaggcctc gaatatacgg gaccgacccc gatgcggggg ttcgcggacc
     3121 ttcagcgtgg g
//
LOCUS       DATCCH010000438         7355 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_453038, whole genome shotgun sequence.
ACCESSION   DATCCH010000438 DATCCH010000000
VERSION     DATCCH010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7355
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453038"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /gene="rpsF"
                     /locus_tag="VIJ44_08415"
     CDS             <1..352
                     /gene="rpsF"
                     /locus_tag="VIJ44_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEY5165960.1"
                     /translation="AGPKEERPMRPYEVMVIYDAELDDADIRAAVDRATKLLESKGAE
                     LGPVDHWGKRRLAYRLNRRWEGYYVVIQARAEPAATDELNRTLALADEVLRHKVMRIP
                     EAVYGKTLGTPADG"
     gene            415..888
                     /gene="ssb"
                     /locus_tag="VIJ44_08420"
     CDS             415..888
                     /gene="ssb"
                     /locus_tag="VIJ44_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEY5165961.1"
                     /translation="MPDSTVTIVGNVTRDPEMRYTPSGVAKASFGVAVSRRWQNRTSH
                     EWEEQTSFFNVVCWREMAENVCESIGKGARIVVSGRLEQRSWETENGDKRSVVEIVAD
                     DIGPSLRWATAEVKRNERRGGFDSPGGGTGGGGGGQAPAPAERTGADLGEGEEPF"
     gene            889..1134
                     /gene="rpsR"
                     /locus_tag="VIJ44_08425"
                     /pseudo
     CDS             889..1134
                     /gene="rpsR"
                     /locus_tag="VIJ44_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013015421.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
     gene            1350..1796
                     /gene="rplI"
                     /locus_tag="VIJ44_08430"
     CDS             1350..1796
                     /gene="rplI"
                     /locus_tag="VIJ44_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017624959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEY5165962.1"
                     /translation="MKIVLREDVDHLGHKGDLLEVADGYARNFLVPRGLAMKATKGVV
                     AQANAMRRSRSARDARDQAAAEDVAARVGAATITLSVRAGEGGKLFGSVTSIDVAEAV
                     QAQTGVEVDRRAIDLPEPIKELGNAEVPVRFHPEVIATLHLEVVAE"
     gene            2006..3370
                     /gene="dnaB"
                     /locus_tag="VIJ44_08435"
     CDS             2006..3370
                     /gene="dnaB"
                     /locus_tag="VIJ44_08435"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEY5165963.1"
                     /translation="MVQALEPVRRRAGSGRVPPHNLEAEESLLGAMLLSRDAITAAVE
                     AGVEASDFYKPAHTHLFDAVMSLYGQGEPVDPVTVAEELRRADLLDSLGGRQTLLHLQ
                     AATPASANATHYAKIVNELALLRRLIGVAGDIAEMGYDDSQDVVETLDRAESLVFEVA
                     ERRVSESMVKVSDALQETLDQLEALYGSSGDITGVPTGYNDLDHLLLGLQPSNLIVVA
                     ARPGAGKTSLALGAAANVAMEARKPVLFFSMEMGTLELTKRLLAGEARVDARRLQTGN
                     IPEADWTRLSHAVGRLAEAPLFIDDNPHCTVMEMRAKARRTKARHGDLGLVVVDYLQL
                     MTPMAKRAENRQVEVAEISRGLKILARELDCPVMALSQLNRQLEYRADKRPMLADLRE
                     SGSLEQDSDVVVFIYRDDIYNPDSDQRGTAEIIVAKHRNGPTGTTRLAFVDRLTKFAN
                     MAPE"
     gene            complement(3403..3609)
                     /locus_tag="VIJ44_08440"
     CDS             complement(3403..3609)
                     /locus_tag="VIJ44_08440"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEY5165964.1"
                     /translation="MAPGAFSPAHILIVLTVALVVLGPKELPKVVRAGAKAMRELRAL
                     RARLDEELHALIDDEEEAQPGPDG"
     gene            complement(3638..3829)
                     /locus_tag="VIJ44_08445"
     CDS             complement(3638..3829)
                     /locus_tag="VIJ44_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011172713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEY5165965.1"
                     /translation="MIPILGEIIGWEALLVIGLIALLFGSSQLPKLARSVGQASRELK
                     RGLHEEPDSETEPKADKDK"
     gene            complement(4064..5212)
                     /locus_tag="VIJ44_08450"
     CDS             complement(4064..5212)
                     /locus_tag="VIJ44_08450"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEY5165966.1"
                     /translation="MDSATLTNQASEDTGLDHWGGDSYRDGLDRLVHALDTEAGLNPL
                     GELALEEQILANLRNRLRVTAWVEQHPELLDEPVERPIIVLGLPRTGTTLLHELFHRD
                     PANRSLMRWEALDSVPPPDAAHFADDPRVDTERAAAEAMDALNPQFRAIHYEAPDGPT
                     ECVAVLSQDFRSLLWSVVANVPSYTRWLLETDPTPAYDYHRRVLQLLQSGAPGRWALK
                     TPHHALFLDAVVDHYPDARLVMTHRDPVTVVASLCSLAGTLGATFSDIDHADDHVRTW
                     TAVGQTLVERVMDFRDRRGDDLFVDIGYDDLVADPLGTVVRILGHFGELLSEPAAASM
                     QAYLADDPHRQVGRHRYSPQGTGIDRDDIGRRFARYADRFAIPPRVSP"
     gene            5263..5496
                     /locus_tag="VIJ44_08455"
     CDS             5263..5496
                     /locus_tag="VIJ44_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165967.1"
                     /translation="MHARYPYPDRSIADKGAFMLAISTMGATPTAIFYIVGFALFILA
                     GIGFKPAGDRVHLVGLGLAALAFVSAWNALAAT"
     gene            complement(5529..6188)
                     /gene="nucS"
                     /locus_tag="VIJ44_08460"
     CDS             complement(5529..6188)
                     /gene="nucS"
                     /locus_tag="VIJ44_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015804815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease NucS"
                     /protein_id="HEY5165968.1"
                     /translation="MRVVVARCSVEYAGRLGARLPPALRLVILKADGSIAIHADAKAY
                     KPLNWMSPPCTISEVDGVIIASNRGGEELRVQLHEVVFDSCFELGDDPGLAKDGVEAE
                     LQALLAGSVHVLGDGLRLVRREYPTDLGPVDLLCRDGLGRAVAVEIKRVGEIAGVEQL
                     LRYQERLDRDGSLAPTRAMLAATRIKPQARVFAASRGVECVEVNLDELRSGAPADLRL
                     F"
     gene            6273..6794
                     /locus_tag="VIJ44_08465"
     CDS             6273..6794
                     /locus_tag="VIJ44_08465"
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HEY5165969.1"
                     /translation="MLATYNSDAYNIVKVLHILCAIVGFGAVTLNGLYGAQAKKRGGS
                     EAAAIGQAVYRVSAVGEYFIYAVFVLGIGLVAMGNNLFDFGQTWVWLSMVLYITGLGL
                     SHGVLRPRVRRMNALMVELAGSSGSAPGGPPPQAVQLKALAKQVATTEMAVNLLLVVI
                     LVFMVFKPGGHSI"
     gene            6843..7118
                     /locus_tag="VIJ44_08470"
     CDS             6843..7118
                     /locus_tag="VIJ44_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165970.1"
                     /translation="MDFYTEVLLELMAAMGAALFVANALALVRRRSDARQAARDAVRK
                     SRPGSPVRKQARLATTGTLAQAPLARTVTFMLLGLVVAIWGIASLTT"
BASE COUNT         1264 a         2538 c         2461 g         1092 t
ORIGIN      
        1 cgccggtccg aaggaggaac ggccaatgcg gccgtacgaa gtcatggtca tctacgacgc
       61 cgaactcgac gacgccgaca tccgggccgc cgtcgaccgg gcaaccaagt tgctcgaatc
      121 caagggcgcc gagctcggac ccgtcgatca ttggggcaag cgccgtctgg cgtaccgctt
      181 gaatcggcgc tgggagggct actacgtcgt catccaagcc cgcgccgagc ccgccgccac
      241 cgacgaactg aaccgcacgc tggccctcgc cgacgaggtg ctccgccaca aggtgatgcg
      301 gatcccagaa gcggtctacg gcaagacgct cggcacccca gccgacggat aggcaccacc
      361 cacgcggcac cgcgccccag gccggacgcc gcaccgcacg aggaggtcac agcgatgccc
      421 gatagcaccg tcacgatcgt cgggaacgtc acgcgcgacc ccgagatgcg ctacacgccg
      481 agcggtgtcg ccaaggcgtc gttcggtgtc gccgtgagcc gccggtggca gaaccgaacc
      541 agccacgagt gggaggagca gaccagcttc ttcaacgtcg tgtgctggcg cgagatggcc
      601 gagaacgtgt gcgaatccat tggcaaaggc gcccgcatcg tcgtcagtgg tcgtctcgag
      661 cagcgctcat gggagaccga gaatggcgac aagcgcagcg tggtcgagat cgtcgccgac
      721 gacatcggtc ccagcctgcg ttgggcgacc gcggaagtga aacgcaacga acgtcgaggc
      781 gggttcgaca gccccggcgg aggaaccggt ggcggtggcg gcggtcaagc gcccgcaccc
      841 gcagagcgaa ccggcgccga cctcggcgag ggggaggaac ccttctagat ggcacgacca
      901 cgacggacca ccaagcgcga tgcccccgag cgcaaaccga agaagaaagt gtcgatcctc
      961 gacaccgagc gaatcgtctg ggttgactac aaggacgtgg ccctcctccg ccgcttcatg
     1021 tcggagcgag ccaagatccg ggcgcgccgc gtcaccggca acgacgcgca gcagcagcgg
     1081 gccgtggcgc gagcgatccg agtcgcgcgc gagatggcgc tgctccccta ctcggtgcgc
     1141 caggtaaccc atcgcaacaa gggaaagcgc gaccgtggcg acggcggggt gttggcggcc
     1201 ccgcgcaacg agcccatgcc gaccccgccc gaaggcgaca ccggccccgg cgagcccccc
     1261 gcggacatcg agacacccga ggccaccgag ctggacgcca cgacccccga gctggtcgcg
     1321 ccgacggaac cgaccgtcga ggaatcggca tgaagatcgt tctccgcgag gacgtcgatc
     1381 accttgggca caagggtgac ctgctcgagg tcgcggacgg ctacgcccgc aacttcctgg
     1441 tgccccgcgg gctggccatg aaggccacga aaggcgtcgt cgcgcaggcc aacgcgatgc
     1501 ggcgcagccg gtccgctcgt gacgctcgcg accaagctgc ggccgaggat gtcgcagctc
     1561 gggtcggtgc cgcgacgatc acgctcagcg tccgcgccgg ggagggcggc aagcttttcg
     1621 gctccgtgac cagcatcgac gtggccgagg cggtccaagc ccagacgggc gtcgaagtgg
     1681 accggcgcgc catcgacctg cccgagccca tcaaagagct ggggaacgcc gaggtaccgg
     1741 ttcggttcca ccccgaggtc atcgccaccc tccatctcga ggttgtcgcc gaataggccg
     1801 gctggggggt gtcacacctc ccgaacacaa tcaacgggtg aacaagttct ccacacccct
     1861 ggggatgagc agccccgtca tccaccgcaa catcacaggg gtttcgctct accaatccac
     1921 aggcagcggg gtccatgctg gccgttccgg tgcggggctg aacgcccggt aaacgaagaa
     1981 ccaacggggg agcggggcgg tcgaggtggt ccaagcactc gaaccagtgc ggcgccgtgc
     2041 cggaagcggc cgcgtcccac cgcacaacct cgaggccgaa gaatcactgc tcggagcgat
     2101 gctcctctcg cgggacgcga tcacggccgc ggtcgaagct ggcgtcgagg catccgactt
     2161 ctacaagccg gcgcacacgc acctcttcga cgccgtgatg tcgctgtacg gccaaggcga
     2221 gcccgtcgac ccggtcaccg tcgccgaaga gcttcgacgc gctgacctcc tcgactcgct
     2281 cgggggacgt cagaccctcc tgcacctgca ggcggccacg ccggcgtccg cgaacgccac
     2341 gcactacgcc aagatcgtca acgagctcgc gctgctccgg cgcctcatcg gcgtcgccgg
     2401 cgacatcgcc gagatgggct acgacgactc ccaagacgtc gtcgagacgc tcgaccgcgc
     2461 cgagtcgttg gtgttcgaag tcgcggagcg gcgcgtgtcc gaatcgatgg tcaaggtctc
     2521 cgacgcgctc caagagaccc tcgaccagct cgaggccctc tacggcagca gcggtgacat
     2581 caccggagtc ccaaccggat acaacgacct cgaccatctc ctgctcggcc tccagccctc
     2641 gaacctgatc gtggtcgcgg cacgtcctgg tgccggcaag acgagcctgg ccctcggcgc
     2701 cgccgccaat gtcgcgatgg aagcacgcaa gccggtcctg ttcttctcga tggagatggg
     2761 cacgctcgaa ctcaccaagc gactgctcgc cggtgaagcc cgggtggacg cacgtcggct
     2821 ccagaccgga aacattcccg aagccgactg gacccggttg agccacgccg tcgggcggct
     2881 cgcggaggcc ccgttgttca tcgacgacaa cccccactgc accgtgatgg agatgcgggc
     2941 caaggctcgg cgaacgaagg cccgccacgg cgacctcgga cttgtggtcg tcgactacct
     3001 gcaactgatg actccgatgg cgaaacgagc cgagaaccgc caagtcgaag tggccgagat
     3061 ttcccggggt ctcaagatcc tcgctcgcga gctggactgt ccggtcatgg ccctgtcgca
     3121 gctcaaccgc cagctcgagt atcgggccga caagcgcccc atgctcgccg acctgcggga
     3181 gtcgggctcg ctcgagcagg actccgacgt cgtggtgttc atctaccgcg acgacatcta
     3241 caaccccgac tccgaccagc ggggcacggc cgagatcatc gtggccaagc accgcaacgg
     3301 cccgaccggg acgacgcgtc tggcgttcgt cgatcgactc accaaattcg ccaacatggc
     3361 acccgagtaa gcggatccga ccgacgacgg ctacccgacc ggtcagccgt cgggaccggg
     3421 ctgggcttct tcctcgtcgt cgatgagggc gtgcagttcc tcgtcgagcc gggcccggag
     3481 ggcccgcagc tctcgcatcg ctttcgcgcc cgcccgcacg accttcggga gctccttcgg
     3541 gccgaggacg acgagggcca cggtcaagac gatcaggatg tgggcgggcg agaacgcacc
     3601 gggagccatg ggtcagtcct gccctcgtga acgagggcta cttgtccttg tcggctttgg
     3661 gctcggtttc ggagtcgggc tcctcgtgga ggccccgttt gagctcacgg ctcgcctgtc
     3721 cgaccgagcg ggccagcttg ggaagctggg agctgccgaa cagcaaggcg atcagtccga
     3781 tcacgaggag cgcctcccag ccgatgattt caccgaggat cggaatcatg gggtcctcct
     3841 tgcaggacac tcgtcctgcg ctggggttgt gagagccctg gttggcgtct ccctaaggta
     3901 cgccaccgca ggcagcgatg gatcaccgcg tggtggagcc accggtcggc cctcgccgcg
     3961 ggcccagcgg agcgtgccgt gtggtccacg tcctgagcat cgggttctcg gatcacggtc
     4021 cggcgagacg ggcgtgatga cgggatgaag gtcccgcgcc cgctcagggc gacacccgcg
     4081 gtgggatcgc gaatcggtcg gcgtagcggg cgaaccgccg cccgatgtca tcgcggtcga
     4141 taccggtgcc ttgcggtgag taccggtggc gccccacctg gcgatgggga tcgtcggcga
     4201 gataggcctg catgctggcg gcggctggtt cgctgagcaa ctcgccgaag tgcccgagga
     4261 tccgcacgac ggtgccgaga gggtcggcga cgaggtcgtc gtacccgata tcgacgaaca
     4321 ggtcatcgcc gcgtcgatcc cggaagtcca tcacccgttc cacgagggtc tggcccactg
     4381 cggtccaggt tcggacgtgg tcgtcggcgt gatcgatgtc actgaacgtc gcgcccaggg
     4441 tgccggccag gctgcacagc gacgcgacga ccgttaccgg gtcgcggtga gtcatgacca
     4501 accgggcgtc ggggtagtgg tcgacgaccg cgtcgaggaa cagcgcgtgg tggggcgtct
     4561 tgagggccca tcgtcccggg gcgccggact gcaagagctg caagacccga cggtgatagt
     4621 cgtacgccgg cgtggggtcg gtctcgagca gccagcgcgt gtaagagggg acattggcga
     4681 cgaccgacca caacaggctg cggaagtcct gggacagcac cgcgacacac tcggtggggc
     4741 cgtcgggcgc ctcgtagtgg atggctcgaa actgcgggtt gagggcgtcc atggcctcgg
     4801 cggcagcacg ctcggtgtcg acccgggggt cgtcagcgaa atgcgcggcg tccgggggtg
     4861 gcacggagtc gagcgcctcc cagcgcatca gcgaccggtt ggcgggatcg cggtggaaga
     4921 gctcatggag gagcgtcgtg ccggtgcgag gcaagcccag cacgatgatc ggtcgctcca
     4981 ccggctcgtc gagaagctcg ggatgctgct cgacccaggc ggtgacgcgc agccggttgc
     5041 gcagattcgc gaggatctgt tcttcgaggg ccagctcgcc gagggggttc aatcccgctt
     5101 cggtgtcgag ggcgtggacg agccgatcca gaccgtcccg atacgaatcg ccaccccagt
     5161 gatcgagtcc ggtgtcctcg ctggcctggt tcgttagggt cgccgagtcc acgtccgaat
     5221 gttctccact ggttctcagg ggcgtggtcc gatatccacc agttgcacgc ccgttatccc
     5281 taccctgacc gaagcattgc cgacaagggg gctttcatgc ttgccatcag caccatgggt
     5341 gcgacaccca ccgcgatctt ctacatcgtt ggtttcgcac tcttcatctt ggccggaatc
     5401 ggcttcaagc cggccggcga ccgcgtgcat ctcgtcgggc tcgggctggc ggcgctcgcg
     5461 ttcgtatcgg cctggaacgc gctcgcggcc acctgacctg acacgccccg gccggggcgt
     5521 cgcccgacct agaacagccg gaggtcggcg ggcgccccgg atcggagctc gtcgaggttc
     5581 acctcgacgc attcgacacc gcgactggcg gcgaagaccc gcgcctgggg tttgatgcgc
     5641 gtcgcggcca gcatggctcg ggttggcgcg agcgacccgt cacggtcgag ccgctcctgg
     5701 tagcgaagga gctgctcgac ccccgcgatc tcgccgacgc gtttgatctc cacggcgacg
     5761 gcgcggccca gcccgtcccg acacagcagg tcaactggtc cgagatcggt ggggtactcg
     5821 cgtcggacca ggcggaggcc gtcgccgagc acatggacgc tgcccgcgag cagcgcctgc
     5881 agttccgctt cgacgccgtc cttggccaac cccgggtcgt cgccgagctc gaagcacgaa
     5941 tcgaatacga cctcgtggag ttgcactcgg agctcctcgc cgccgcggtt gctggcgatg
     6001 atcaccccgt ctacctcgct gatcgtgcac ggcgggctca tccagttgag cggtttgtag
     6061 gccttcgcat ccgcgtggat cgcgatcgac ccgtcggcct tcagaatcac cagccgcaac
     6121 gccggcggca aacgagcacc gaggcgaccg gcgtactcca ccgagcagcg ggcgacgacc
     6181 acgcgcacga ggcgggttgt agcacgcgct cgacccccac ggcgcctacg atgccgccgc
     6241 gcaccgggag gccaaacgga ggggagagcc ggatgctcgc gacgtacaac agcgacgcat
     6301 acaacatcgt caaggtcctg cacatcctct gcgccatcgt cggcttcggt gccgtaacgc
     6361 tcaacgggct gtacggcgcc caagccaaga agcgaggggg ctcggaagcc gccgcgatcg
     6421 gccaagccgt ctaccgggtc tctgcggtcg gcgagtactt catctacgcc gtcttcgtcc
     6481 tcggcatcgg cctcgtcgcc atgggcaaca acctgttcga cttcggtcag acctgggtgt
     6541 ggctctcgat ggtgctctac atcaccggtc tcgggctgtc ccacggcgtg ctgcggccac
     6601 gggtccggcg gatgaacgcc ttgatggtcg agctcgccgg cagttccggt tccgctcccg
     6661 gcgggccgcc accgcaagcc gtccaactca aagcgctggc caagcaggtg gccacgaccg
     6721 agatggccgt caacctcctg ctggtcgtca tcttggtgtt catggtcttc aaaccgggag
     6781 gccattcgat atagcccgcc tcgggacgcc ggtggtctcc ggcgcagggg taccgtgcgc
     6841 aggtggactt ctataccgag gtacttcttg agctcatggc cgcgatgggc gccgcgctct
     6901 tcgtcgccaa cgcgttggcg ctcgtccggc gccgctccga cgcccgccaa gcagcccggg
     6961 acgcggtgag gaagagccgt cccggaagcc ccgtgcgcaa acaggcccgg ctcgccacga
     7021 ccggcaccct cgcccaggct ccgctcgccc gcaccgtgac cttcatgctc ctcggcctcg
     7081 tcgtcgcgat ctggggcatc gcgagtctca cgacctgacc gaaccggagc gggaacccgc
     7141 gcacccaaaa cggcccgggc catccgactc accgcgggaa ccgtcggaac caaccatgat
     7201 ctggccgtga ggcgggcgcg cggcgaacag cgcgacgttc atcgacgcct acaccccgag
     7261 catcggacat gacgcctgcc agtcgccgtg ccgccgctgg gtcgagccac tcgtacctgc
     7321 ttccccggcg gcgcctgtgc acccgaacct cgccg
//
LOCUS       DATCCH010000439         2094 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_453160, whole genome shotgun sequence.
ACCESSION   DATCCH010000439 DATCCH010000000
VERSION     DATCCH010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453160"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="VIJ44_08475"
     CDS             <1..607
                     /locus_tag="VIJ44_08475"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="activates fatty acids by binding to coenzyme A; in
                     Mycobacterium may be involved in virulence; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEY5165971.1"
                     /translation="GSDLGPARFAVDARVKVLDEDTGREVDPGTDQVGVVAVRGRIPL
                     GYHRDAEKTAQTFRVVDGVRYSIPGDYATVAADGAIQLLGRGSACINTGGEKVYPEEV
                     EQLLREHRDVFDCLVVGVPDDRFGEMVVALVQPEVATSVDAASLAEWCRGRLAGYKRP
                     KRFFAVESLERSAAGKADYRRLRRLAAALADAEGITAPAGS"
     gene            complement(535..>2094)
                     /locus_tag="VIJ44_08480"
     CDS             complement(535..>2094)
                     /locus_tag="VIJ44_08480"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY5165972.1"
                     /translation="QTAFRNRLAVLQLVRVGLAVAVMVLPALVGAPGAPASVPLAYLL
                     VVGAAELGRRRFARLASPLLSALVLVDGAFLAAAILLTGGNDTSPLLFLAFLEVVAVT
                     LLASHRTGLKVAVWCALLLFLGRTAASAGVLHLSHPGPDRAAAVSALAFLLFAVGAAA
                     FQSVNERTLRHTGSELAALVDLGSALERARRPGEVVGVLVAHLRTRLRFARVAVMVRV
                     DGSWLTWGAHAPSGGDTGPLGRVANAVLADGQARLVRTVDDGLLAAQLPGAHNVVVAS
                     LTGDEEPLGLVAAEWGHGAQARIPGLTVSSLLQSVAHAALALRNARLLDEVERLATRD
                     EVTGLANRRLFEETFPLEVGRHDRTGAPLSLVVLDVDNFKAINDEFGHPVGDGVLRDV
                     GHALAANTKAFDLAARYGGDEFVVLLPGCSAADAPRVAERMRAAASRAVQGPTAVALS
                     AGAATMPNNAGDGAQLLAAADEALYKAKRGGRDRVETAHPLEPSSTQEPAGAVIPSAS
                     ASAAARRRNRR"
BASE COUNT          327 a          760 c          751 g          256 t
ORIGIN      
        1 cggttcggat ctcggtccgg cccgcttcgc ggtcgacgcc cgggtgaagg tcctcgacga
       61 ggacaccggg cgtgaggtcg accccggaac cgatcaggtc ggcgtcgtcg cggtccgcgg
      121 ccgaatcccc ctcggctacc accgcgacgc cgagaagacc gcgcagacgt tccgcgtcgt
      181 cgacggggtg cgctactcga tccccggtga ctacgcgacc gtggccgccg acggcgcgat
      241 ccagctcctc ggtcggggct cggcctgcat caataccggc ggtgagaagg tgtaccccga
      301 agaggtggag cagctgctcc gcgagcatcg ggacgtgttc gactgcctgg tcgtgggcgt
      361 ccccgacgac cggttcggcg agatggtggt cgcgctggtg caaccggagg tcgcgacgtc
      421 ggtcgacgcg gcgtcactgg cggagtggtg tcgcggtcgc ctcgcgggct acaagcgacc
      481 gaagcggttc ttcgccgtcg agagtctgga gcgctccgcg gctggcaagg ccgactatcg
      541 gcggttgcgt cgtctcgccg cggcgctggc cgacgccgaa gggatcacgg caccggcggg
      601 ctcctgagtc gacgacggct ccagggggtg tgccgtctcg acccggtcgc gtccacctcg
      661 cttcgcctta tacaaagcct cgtctgccgc ggccagcagc tgagccccat cgcccgcgtt
      721 gttgggcatc gtcgccgcgc ccgcgctcaa cgccactgcg gtcggcccct gcaccgctcg
      781 gctcgccgcg gcccgcatcc gttccgccac ccgaggggcg tccgcggcgg agcacccggg
      841 gagcagcacg acgaactcgt cgccgccata tcgggccgcg agatcgaacg ccttggtgtt
      901 cgccgcgagc gcgtggccga cgtcgcggag gacgccgtca cccacgggat gcccgaactc
      961 gtcgttgatc gccttgaagt tatcgacgtc gaggacaacc aggctgaggg gcgctcccgt
     1021 ccgatcgtgg cgaccaacct cgagcgggaa cgtctcttcg aacagtcgtc ggttggcgag
     1081 gccggtcact tcgtcgcggg tggcgagccg ctccacctcg tcgagcaggc gggcgttgcg
     1141 cagggccagc gccgcgtgcg cgacggattg cagcaacgac gagaccgtca ggccggggat
     1201 ccgggcctgg gccccgtgcc cccactcggc cgcgaccagg ccgagtggct cctcatcccc
     1261 agtcagcgat gcgaccacga cgttgtgcgc cccggggagc tgcgcggcga gcaggccgtc
     1321 gtcgaccgtt cggaccaacc gagcctggcc gtcggcaagg acggcgttgg cgacccgccc
     1381 caggggcccc gtgtcaccgc cggagggggc gtgggcaccc caggtcagcc acgacccgtc
     1441 gacccggacc atgacggcga cccgagcgaa ccgcagccgg gtgcggagat gggccacgag
     1501 gaccccgacg acctcaccgg gccgccgggc ccgttcgagc gccgatccaa gatcaacgag
     1561 cgccgcaagc tccgatccgg tgtgacgcag ggtccgctcg ttcacggact ggaacgcagc
     1621 agcacccacc gcgaagagca agaacgccaa cgcgctcacc gcggcggctc ggtccggacc
     1681 gggatggctc aaatgcagga cgccggcgga ggccgcggtc cggccgagga agagcagcag
     1741 cgcgcaccac accgcgacct tcaggccggt gcggtgcgag gccagcaggg tcaccgccac
     1801 cacctcgagg aacgcgagga acagcaacgg gctggtgtcg ttcccgccgg tgagcaggat
     1861 cgccgcggcg aggaacgccc cgtcgacgag gaccaacgcc gagagcaacg gggacgccag
     1921 gcgggcgaag cgccgccggc caagctcggc cgccccgacg acgagcagat acgccaacgg
     1981 caccgatgcc ggggcgcccg gcgcgccgac gagcgccggg agcaccatga ccgccacggc
     2041 cagccccacc cgtaccagct gcaggacggc cagccggttg cgaaacgccg tctg
//
LOCUS       DATCCH010000440         2614 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_619803, whole genome shotgun sequence.
ACCESSION   DATCCH010000440 DATCCH010000000
VERSION     DATCCH010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619803"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VIJ44_08485"
     CDS             <1..238
                     /locus_tag="VIJ44_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013504641.1"
                     /note="activates fatty acids by binding to coenzyme A;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEY5165973.1"
                     /translation="PDPEMGERVHAVVQPVRAEDGGPDLERELLAFCREHLSHYKCPK
                     AIDFDDELPRQPTGKLYKRLLRDRYWGTKSSRIV"
     gene            235..1026
                     /locus_tag="VIJ44_08490"
     CDS             235..1026
                     /locus_tag="VIJ44_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020540749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03084 family metal-binding protein"
                     /protein_id="HEY5165974.1"
                     /translation="MTGPNYPGLLADLAAEEAALDRVVADLDDTGWATPTPAEGWDVR
                     DSVAHLAFTEDLARAALTDGEVFARRRDALTDAPDPSEVLVGAGRSMTGRAVLEWWRE
                     ARAAVLAQLEAHEPRDRLPWIEGPMSAMSFATARLMETWAHGQDVRDALGREPEVSAR
                     LRHVADLGVRTRPFSYGARGVELPAAEVRVELVGVDGERWVWGPDKVEDVVRGPALDF
                     CLVVTQRVNPSDTALRVQGPLARDWLGIAQAFAGPATEHRPPRRT"
     gene            complement(1036..1854)
                     /locus_tag="VIJ44_08495"
     CDS             complement(1036..1854)
                     /locus_tag="VIJ44_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007668651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY5165975.1"
                     /translation="MSAGSSQPVLVDVVDGVARLTLNRPDASNAISLELAQALADATN
                     ELAAADDVRAVLLRGAGPRFCAGGDVKAFAAVGDALGEELGSVLDALHRAVTDLAELD
                     APVVAAVQGSAAGAGLSLVLGADLVVAGTSARFVLAYTGIGLVPDGGSTWYLARLVGL
                     RRATELALTNRVLDANEALAWGIVTRVVPDDEVDTEAEALVTQLAAGPTGALADAKRL
                     LRRSLQASLAAQLRDEAIAMVRAGESRDGQEGVRAFVEKRPPRFGEDGGDPDRG"
     gene            complement(1851..>2614)
                     /locus_tag="VIJ44_08500"
     CDS             complement(1851..>2614)
                     /locus_tag="VIJ44_08500"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY5165976.1"
                     /translation="ERDGDQWRITGQKVWSSRAAYAHRGFLLARTDPTVPKHTGITAF
                     AIDMAAPGVDVRPLRQMNGDAHFSEVFLDDLAVADSDRIGPIGGGWGVARTALANERG
                     AVGAVSAGTGVSIGRLETLARDRCRSGDPRWRDRLAAAHASSEAARFTRNRARARAEV
                     GQTPGPEGSGAKLRGSATFKATVDLAVGILGPSGVAGSDADALEWRTLFLTAPSISIR
                     GGTDEIQRNIIGEHVLGLPPEPRVDIDRPWNEVPR"
BASE COUNT          361 a          921 c          975 g          357 t
ORIGIN      
        1 ccccgatccc gagatgggcg aacgcgtcca cgcggtggtg caaccggtgc gcgccgagga
       61 cggcggcccg gacctggaac gcgagctcct ggccttctgt cgcgagcacc tgtcgcacta
      121 caagtgcccg aaggccatcg acttcgacga tgagctgccg cgccagccga ccggcaagct
      181 gtacaagcgg ctgctgcggg accgctactg gggcaccaag tcgagccgga tcgtatgacc
      241 gggcccaact atccgggact gctggcagac ctggcggccg aagaagcggc cctggatcgc
      301 gtcgtcgccg atctcgacga caccggctgg gcgacaccga cgccagccga aggctgggac
      361 gtacgcgatt cggtcgcgca cctggcgttc accgaggacc tggcccgcgc cgcgttgacc
      421 gacggggagg ttttcgcgcg acgccgtgac gcgctgacgg acgcaccgga cccgagcgag
      481 gtcctcgtcg gggccggccg gtcgatgacc ggacgggcgg tgctcgagtg gtggcgggag
      541 gcgcgcgccg cggtgctcgc tcagctcgag gcccacgagc cgcgggaccg tctgccgtgg
      601 atcgagggcc cgatgtccgc gatgtcgttc gccaccgccc ggctcatgga gacctgggct
      661 cacggtcaag acgtccgcga tgcgctgggc cgagaacccg aagtcagcgc ccggttgcgc
      721 cacgtcgctg atctcggggt gcggacccga ccgttttcct acggggcgcg aggtgtcgag
      781 ctgcccgccg ccgaggtgcg ggtcgagctg gtcggtgtcg acggtgaacg gtgggtgtgg
      841 gggcccgaca aggtcgagga cgtggtgcgt ggcccggcgc tggacttctg cctggtggtc
      901 acccaacgtg tcaacccgtc cgacaccgca ctccgggtgc aaggtccgtt ggcacgagac
      961 tggctcggaa tcgcgcaggc gttcgcgggt cccgccactg agcaccgtcc gccgcgtcga
     1021 acgtgaccgc tcgggtcatc cgcggtcggg gtcgccgccg tcctcgccga atcgcggcgg
     1081 tcgcttctcg acgaaggccc gaacgccttc ctgcccgtcg cgggattcac cggcgcgtac
     1141 catcgcgatc gcctcgtcac ggagctgcgc cgcgagcgac gcctgcagcg agcggcgcaa
     1201 cagccgcttc gcgtccgcca acgcgccggt cgggcccgcc gcgagctggg tcaccagggc
     1261 ctcggcctcc gtgtcgacct cgtcgtcggg gacgacccgg gtgacgattc cccaggcgag
     1321 ggcctcattg gcgtcgagga cccggttcgt caacgccagc tcggtggctc ggcgcagccc
     1381 cacgaggcgg gcgagatacc aggtggagcc gccgtcggga acgagaccga tccccgtgta
     1441 ggcgaggacg aagcgagccg acgtcccagc cacgacgagg tccgcgccga gcacgaggga
     1501 caggcccgcg cccgcggcgc tgccctggac cgcggcgacg accggggcgt cgagctcggc
     1561 gaggtcggta accgcccggt gcagcgcgtc gaggaccgag cccagctcct ccccgagggc
     1621 gtcaccgacc gcggcgaagg ccttcacgtc acctccggca cagaatcggg gtccggcccc
     1681 gcgcagcagc accgcccgaa catcgtcggc ggcggcgagc tcgttcgtgg cgtcggccaa
     1741 cgcctgggcg agttcgaggc tgatggcgtt cgaggcatcc ggccggttca gggtcaggcg
     1801 cgccacgccg tcgacgacgt cgaccaggac gggctgggat gacccggcgc tcatcgcggc
     1861 acctcgttcc agggtcggtc gatgtcgaca cgcggctccg gcggaaggcc gagtacgtgc
     1921 tccccgatga tgttgcgctg gatctcgtcg gtgccgccgc ggatcgagat cgacggtgcg
     1981 gtgaggaaca gggtgcgcca ctccaaggcg tcggcgtcgg aaccggcgac gccactcgga
     2041 ccgaggatcc cgaccgcgag gtcgacggtg gctttgaacg ttgcgctgcc gcgcagcttc
     2101 gcccccgatc cctcgggtcc cggggtctgc ccgacttcgg cgcgggcacg ggcccggttg
     2161 cgggtgaagc gcgccgcctc gctcgaggcg tgcgcggcgg ccaggcgatc ccgccaccgc
     2221 gggtcgccgg accggcaccg atctcgggcc agcgtctcga gccggccgat ggacacgccc
     2281 gtcccggcgc tgacggcacc gacggcgccc cgttcgttcg ccagcgcggt gcgggccacc
     2341 ccccagccgc cgccgatggg gccgatgcgg tcggagtcgg cgacggcgag gtcgtcgagg
     2401 aacacctcgc tgaagtgcgc gtcgccgttc atctgacgga ggggtcggac gtcgacgccg
     2461 ggtgcggcca tgtcaatggc gaacgcggtg atcccggtgt gcttcggaac cgtggggtcg
     2521 gtgcgggcga ggaggaaccc ccggtgggcg tacgcggccc ggctgctcca gaccttctgg
     2581 ccggtgatac gccattggtc gccgtcgcgc tcgg
//
LOCUS       DATCCH010000441         2947 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_619850, whole genome shotgun sequence.
ACCESSION   DATCCH010000441 DATCCH010000000
VERSION     DATCCH010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619850"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="VIJ44_08505"
     CDS             <1..764
                     /locus_tag="VIJ44_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003988070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEY5165977.1"
                     /translation="PANEATLAEVCVVACAEAWRGDGEIVVSPIGVIPTLGARLAALT
                     FEPDLVYTDGDALQLSAPIPIGVDAEKHVEAWLPFAKMFDILWAGRRHVMMGATQVDR
                     FGNQNIACIGPWARPKAQLLGVRGAPGNTVNHATSYWVPNHSPRVFVPTVDCVSGVGY
                     DRAAAAGPAASEYHEIRRVISNLGVFDFETPDHAMRIRSVHPGVTVEAILEATGFPLV
                     VPDQVPTTAAPTPEDVRLLDDVLDPSGLRSAELPG"
     gene            764..1819
                     /locus_tag="VIJ44_08510"
     CDS             764..1819
                     /locus_tag="VIJ44_08510"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018720042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEY5165978.1"
                     /translation="MHPTLHTELCDLCGIRVPVVQTGMGWVAGPRLTAATANAGGLGI
                     LAAGTMDFEQLRAAIAETRSRTDAPFGVNLRSDAPDASARCELLIREGVKVASFALAP
                     QQALIARLKEAGVVVIPSIGARRHAEKVAAWGVDAVLVQGGEGGGHTGAVPTSLLLPQ
                     VVDTVDIPVIAAGGFFDGRGLVAALAYGAAGVAMGTRFLLTTDSPVGDEVKALYLAKS
                     VTDTLVTTQVDGVPHRVLRTDFTERLDRGRPLTSLARAVRNALRFQHLSGTRWRDMLH
                     EGAGMRRTQELSWNQVVMAANTPMLLKAAMVDGRPDLGVMSSGQVVGVIDDLPSVAEL
                     IDRIVHDAEAVLKRLGA"
     gene            1816..>2947
                     /locus_tag="VIJ44_08515"
     CDS             1816..>2947
                     /locus_tag="VIJ44_08515"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY5165979.1"
                     /translation="MSTVQSTDGLVEPGFGPVADAFRANFDDHDDVGAACCVYLDGRP
                     VVDLWGGLADRASARAWARDTIVIVFSCTKGVTAIAANQLIAAGLLNPDAPIADYWPE
                     FAANGKAAITVGMALSHRAGLAAIDGDLTLADIAAWDPVVAAIAAQAPNWEPGTAHGY
                     HSRSYGWIVGELIRRVSGQASPGAYIAQHIAEPLGLDFVLGVPEDQDARVATLYPAPN
                     ADALDQFAAAAPESLFARVFTGPSGLLRYDDQWNTRLLRAAQLPSSNGHGRATALARM
                     YAACLQPLDGVRLLSDDALDQATEVRSDGPDVVLGQPLTFGLGFALPPTLGPGAGPRS
                     FGHPGAGGSLGFADRDRGLAFAYVMNQMRFDASGADPRAASLS"
BASE COUNT          402 a         1155 c          963 g          427 t
ORIGIN      
        1 gcccggcgaa cgaagccacc ctcgccgagg tgtgtgtcgt cgcgtgcgcg gaggcgtggc
       61 gcggcgacgg cgagatcgtc gtcagcccca tcggcgtcat cccgacgttg ggcgcccgcc
      121 tcgcggcgct gaccttcgaa cccgacctcg tgtacaccga cggcgacgcg ctccagctca
      181 gcgcaccgat ccccatcggt gtcgacgccg agaagcatgt cgaggcgtgg ctgccgttcg
      241 ccaagatgtt cgacatcttg tgggccggac gccgtcacgt gatgatgggc gcgacccaag
      301 tcgaccggtt cggcaaccag aacatcgcct gcatcggccc gtgggcgcga cccaaagccc
      361 agctgctcgg ggtgcgcggg gcacccggca acacggtcaa ccacgccacg agctactggg
      421 tgcccaatca ctctccccgc gtgttcgtcc cgaccgtgga ctgcgtctcc ggcgtcgggt
      481 acgaccgggc cgcggcggcc gggcccgcgg cgtcggagta ccacgagatc cgtcgcgtga
      541 tctccaacct cggggtcttc gacttcgaga cccccgacca cgccatgcgg atccgctcgg
      601 tgcatccggg cgtcaccgtc gaagccatcc tcgaggccac cggcttcccg ctcgtcgtgc
      661 ccgaccaggt ccccaccacg gcggctccga cccccgagga cgttcgcctc ctcgacgacg
      721 tcctcgatcc gagcgggctg cggtcggccg agctacccgg ctgatgcacc cgacgttgca
      781 caccgagctg tgcgacctct gcgggatccg tgtccccgtc gtgcagaccg ggatgggctg
      841 ggtggccggc cctcgcctca ccgccgcgac cgcgaacgcc ggcggtcttg gcatcttggc
      901 cgccgggacc atggacttcg agcagctacg cgccgcgatc gccgagaccc ggtcccgcac
      961 cgacgcgccg ttcggcgtca acctgcgctc cgacgctccc gacgcctccg cccggtgcga
     1021 gctgctcatc cgcgaaggcg tgaaggtggc gtcgttcgcc ctcgcgcccc aacaggccct
     1081 gatcgcccgc ctcaaggagg ccggcgtggt cgtgatcccc tccatcggtg cccgccgcca
     1141 cgccgagaag gtcgcggcgt ggggcgtcga cgcggtgctc gtccaaggcg gcgaaggagg
     1201 cggccacacc ggggcggtcc caacctcgct gctgttgccc caagtcgtcg acaccgtcga
     1261 catcccggtc atcgccgccg gcggcttctt cgacgggcgc ggcctggtcg cggcgttggc
     1321 gtacggcgcg gcgggcgtcg ccatgggcac ccgcttcctc ttgacgacgg acagcccggt
     1381 tggcgacgag gtcaaggcgc tctacctcgc caagagcgtg accgacacgc tcgtcaccac
     1441 ccaggtcgac ggggttccgc accgggtgct gcgcaccgac ttcaccgaac gcctcgaccg
     1501 cggccgcccg ctcaccagcc tggcccgggc ggtccgcaac gcgctgcggt tccagcacct
     1561 ctcggggacc cgctggcgcg acatgctgca cgaaggcgcc gggatgcgcc gaacccagga
     1621 gctctcgtgg aaccaggtcg tgatggcggc caacaccccg atgctgctca aagcggcgat
     1681 ggtcgacggc cgacccgacc tcggcgtcat gtccagcggc caggtcgtcg gtgtcatcga
     1741 cgacctaccg agcgtcgccg agctcatcga ccgtatcgtc cacgacgccg aagccgtcct
     1801 caaacggctg ggcgcatgag caccgttcag tccaccgacg ggctcgtcga gcccggcttc
     1861 ggccccgtcg ccgacgcgtt ccgggccaac ttcgacgacc acgacgacgt cggcgccgcc
     1921 tgctgcgtgt acctcgacgg tcgaccggtt gtcgacctct ggggtggcct cgccgaccgg
     1981 gcctcggcgc gcgcttgggc ccgcgacacg atcgtcatcg tcttctcgtg caccaagggc
     2041 gtcaccgcga tcgcggccaa ccagctcatc gccgccgggc tcctcaaccc tgacgcgccg
     2101 atcgccgact actggcccga gttcgccgcc aacggcaaag ccgcgatcac cgtcgggatg
     2161 gcgctctccc accgggcagg gctcgccgcc atcgacggtg atctcaccct ggccgacatc
     2221 gcggcgtggg atcccgtcgt cgccgccatc gccgcccagg cccccaactg ggagcccggc
     2281 accgcccacg gctaccactc ccgctcctac ggctggatcg tcggcgagct gatccgccgc
     2341 gtctcaggtc aggccagtcc cggcgcgtac atcgcgcagc acatcgcgga accgctcgga
     2401 ctcgacttcg tcctcggggt ccccgaggac caagacgccc gagtggcgac cctgtacccg
     2461 gcgcccaacg ccgacgcgct cgaccagttc gccgcggcag cccccgagtc gctcttcgcc
     2521 cgcgtcttca ccggcccgtc cggcttgttg cgctacgacg accaatggaa cacccggctg
     2581 ctgcgcgccg cgcagctccc gtcgtcgaac gggcatggcc gcgccaccgc gttggcccgg
     2641 atgtacgcgg cgtgcctgca acccctcgac ggggtgcggc tcctctccga cgacgcgctc
     2701 gaccaggcga ccgaggtccg ctccgacggg cccgacgtcg tgctcggcca gccgctcacc
     2761 ttcgggctcg gcttcgcgct ccccccgacg ttggggccgg gggcgggacc ccgatcgttc
     2821 gggcaccccg gcgcgggagg atcgctcggc ttcgccgacc gcgaccgtgg cttggcgttc
     2881 gcctacgtca tgaaccagat gcgcttcgac gccagcggtg ctgacccgcg ggcggcgtcg
     2941 ctcagcg
//
LOCUS       DATCCH010000442         4597 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_245403, whole genome shotgun sequence.
ACCESSION   DATCCH010000442 DATCCH010000000
VERSION     DATCCH010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4597
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245403"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="VIJ44_08520"
     CDS             <1..631
                     /locus_tag="VIJ44_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010640857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY5165980.1"
                     /translation="RVVGIDASSDVIAEANELRDAAGPDAANARFVVGDVYDLDFADA
                     SFDVVHAHQVLQHLSEPVRALSSMRRVLKPGGALGVRDGDYAGFVWAPIDRLLDRWME
                     LYHLITRRNGGEADAGRYLLGWVQQAGFVDMVATSSTWTFADPERRAWWGGLWADRVE
                     QSSFAEQAIQYGLSDRAELGAIALAWRRWAERPDAYFAVLHAEVLARRG"
     gene            739..1353
                     /locus_tag="VIJ44_08525"
     CDS             739..1353
                     /locus_tag="VIJ44_08525"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018178584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY5165981.1"
                     /translation="MGVYSDQVVPRLTNRLLGTKEFGKLRREACEGLHGDVIEVGFGS
                     GLNVAHLPDAVTGVWAVEPSGTATKLAAKRMAASPTPVHVVGLDGARLEFPDHRFDAA
                     LSTMTLCTIPDVVAALQELRRVLKPGATFHFAEHGRAPVAKVARAQDRFNAMQMRLAG
                     GCHLNRDVAALITEAGFEIEQLRTFFLKGPKAWGYMSIGWARSH"
     gene            complement(1464..1760)
                     /locus_tag="VIJ44_08530"
     CDS             complement(1464..1760)
                     /locus_tag="VIJ44_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165982.1"
                     /translation="MSGPRRRKPPPQRSRRRRAASARDFWGSEPTDDPPADVIAASDD
                     PTVMIRSLGPPPFPGNETIAEHYFAATYDKAAALAVALAAASGLLATEDPTPDA"
     gene            complement(1757..>4597)
                     /locus_tag="VIJ44_08535"
     CDS             complement(1757..>4597)
                     /locus_tag="VIJ44_08535"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY5165983.1"
                     /translation="AWAGGPGAPSADGDEVLAMLTAAGAPASGWIPHGVVVLPGASTA
                     DALATPVGEVLGWLVAAGAGQAGDDLGPSAHWLGRVAIWAVELTARGAVVPVLRQRRR
                     GNGSTSASSGSYSVRWTPALIDPTRLTQVASTMPGTVLALDPHVDRRALTRSALTGMV
                     GAICRDGARRLEVPAPPPRVRTAADVAEAFLARLDGSAFDAPVRIAGEISARGERWAR
                     SVTREHAPLVVRLDPPDDRGAWHLAVLAPGPDGALTPIEQAIVNAGSDRSAFEDELTR
                     LERMLPALLRPGSTRRGQVILSQDEAWELMVTTGPRLSGAGFDVRVPALSRRRPSPTL
                     RVFADPSSTTTVGANQLANVRWSALFDDVELTADDIARLAKEAQPLIRSGGRWVAIDQ
                     ADLQAAAETLAERAATTQLSGAEMLRLALGLEGSPLSGGISVAGGGWAADLLSSAANL
                     SAEPAGAPDGFVGDLRDYQAEALAWLGFLDSAGLGGCLALDMGLGKTPTMLAHLLAGA
                     AEGPALVIAPAAVVGNWAAEAARFTPGLRVVVHHGTNRASPDEIAAEVDGADVVITTY
                     GTAVRDVDAIADVEWARLVLDEAQAVKNPTNETSQQLRRIPARTRVALTGTPIENGLG
                     DLWAILDFTNPGLVGPRPQFIAHLSHEGGATAAAEDALRALNGILVFRRTKAEPVIAA
                     ELPDRIDELDHCSMTPEQIGMYQAVLDKLVIGTGGPEGEEPRKGQILAAITALKQICN
                     HPAAYQDDDRPLEGRSGKLARLEEIVAAVFAAGERLLVFTHFAEWGQHLAEHLTERTG
                     TPVACYHGGLSRTVRDRLIADFQAGEGPGALVLSLKAGGTGLNLTNASHVVLYDRWWN
                     PAVEDQARDRAWRIGQTRTVICHRLICPGTVDERVEEVVAGKRRIADLVLAKSTSLGD
                     LDSTQLRVALGIRPDAVLTEDLALTDEDGAAA"
BASE COUNT          618 a         1502 c         1776 g          701 t
ORIGIN      
        1 ccgcgtcgtt ggcatcgacg cttcgagcga cgtgatcgct gaggccaacg aactccggga
       61 cgccgccggt cccgatgccg ccaacgcccg gttcgtcgtt ggggacgtgt acgacctcga
      121 cttcgctgac gcgtcgttcg atgtcgtgca cgcccaccag gtactccaac acctgtccga
      181 gccggtgcgg gccctgtcct ccatgcggcg cgtcttgaaa cccggcgggg ccctcggggt
      241 ccgggacggc gactacgccg gattcgtctg ggcgccgatc gaccggctcc tcgaccggtg
      301 gatggagctc taccacctca tcacccggcg taacggcggc gaggccgacg ccgggcgcta
      361 cctgctcggc tgggtgcaac aggccgggtt cgtggacatg gtcgcgacca gctcgacctg
      421 gacgttcgcc gaccccgagc gtcgggcttg gtggggcggc ctctgggccg accgagtcga
      481 gcagtcgtcg ttcgcggagc aggcgattca gtacgggctg agcgaccggg ccgagctggg
      541 cgcgatcgcg ctggcgtggc gccgatgggc ggagcggcca gatgcctact tcgcggtgct
      601 gcacgccgag gtcctcgccc gacggggata acggatctcc gcgtgttcga ggttctcttc
      661 ggcgccgtgg ccctcacggg cttctcgttc ggcccgtgac cgggcgtgcc ggtcgcgctc
      721 cgccggtacc ctgccgacgt gggcgtctac agcgatcagg tcgtgccccg gctcacgaac
      781 cggttgctcg gcacgaagga gttcgggaag ctgcggcgcg aggcatgtga aggcctgcac
      841 ggtgacgtca tcgaggtggg attcgggtcg gggctcaacg tggcgcacct gccggacgcg
      901 gtgacgggcg tctgggccgt cgagccctcg ggaacggcaa ccaagctggc tgcgaagcgg
      961 atggccgcgt cgccgacacc ggtgcacgtg gtgggtctcg acggggcgcg gctcgagttc
     1021 ccggatcacc gcttcgacgc ggcactgtcg acgatgacgc tctgcaccat ccctgacgtc
     1081 gtcgccgccc tccaggagct gcgacgggtg ctgaaaccgg gagccacctt ccacttcgcg
     1141 gagcatggcc gcgcgccggt cgcgaaggtc gctcgcgctc aggaccggtt caatgcgatg
     1201 cagatgcgcc tcgcgggcgg ctgtcacctg aaccgcgacg tcgccgcgct catcacggag
     1261 gccggtttcg agatcgaaca gctccgcacc ttcttcttga aggggccaaa ggcgtggggc
     1321 tacatgtcga tcgggtgggc ccggtcgcat tgacgcgccc gggtcattgg cccggtgagc
     1381 cacggtcgcc gaccggccga gctggtgatc ctcgaacggc gcgagcgggg gcgagtcgct
     1441 cgaccgcggg tcccggtcgg agctcaggcg tccggcgtcg ggtcttcggt ggcgaggagt
     1501 cccgaggcgg ccgcgagcgc gaccgcgagc gcggcggcct tgtcgtaggt cgcggcgaag
     1561 taatgctcgg cgatcgtctc gttgccgggg aacggtgggg gcccgaggga tcggatcatg
     1621 acggtgggat catccgacgc tgcgatgacg tcggcgggcg ggtcgtcggt gggctcgctg
     1681 ccccagaagt cacgcgcgga ggccgcgcgg cgacggcggc tccgttgcgg gggaggcttg
     1741 cggcgacgcg gtccgctcat gcggcggctc catcctcgtc ggtcaacgcc aggtcctcgg
     1801 tgagcaccgc gtcgggtcgg atgccgagcg ccacccggag ctgggtgctg tcgaggtcgc
     1861 ccaacgacgt cgacttcgcc aggaccagat cagcgatacg gcgtttgccc gccacgacct
     1921 cctcgacccg ctcatcgacg gtgccgggac agatgagccg gtgacagatc acggtgcggg
     1981 tctggccgat gcgccacgct cggtctcggg cttggtcctc cacggctggg ttccaccagc
     2041 ggtcatagag gacgacgtgg ctggcgttgg tcaggttcaa ccccgtcccg ccggccttca
     2101 acgacagcac cagggccccg ggtccctcgc ccgcctggaa gtcggcgatg aggcggtccc
     2161 gcacggtgcg cgacagcccg ccgtggtagc aggccaccgg cgtgccggtg cgctcggtga
     2221 gatgctcggc gaggtgctga ccccattcgg cgaagtgggt gaacacgagg agtcgttcgc
     2281 cggcggcgaa caccgcggcg acaatctcct cgaggcgggc cagcttgccc gagcgaccct
     2341 cgagcggacg gtcatcgtct tgatacgcgg ccgggtggtt acagatctgt ttgagggctg
     2401 tgatcgcggc gaggatctga ccctttcggg gttcctcacc ctcgggaccg ccggtgccga
     2461 tcaccagctt gtcgaggacc gcctggtaca tcccgatctg ttcgggggtc atcgagcagt
     2521 ggtcgagctc gtcgatccgg tcggggagct cggccgcgat gaccggctcg gccttggtgc
     2581 gtcggaagac gaggattccg ttcagggctc ggagcgcgtc ctcggcggcg gcggtggcgc
     2641 caccctcgtg ggagaggtgg gcgatgaatt gtggtcgggg gccgacgagc ccagggttgg
     2701 tgaagtcgag gatggcccac aggtcgccga ggccgttctc gatgggggtg ccggtgagcg
     2761 cgacccgggt tcgggcgggg atccggcgca gctgttgtga ggtctcgttc gtggggttct
     2821 tgaccgcctg ggcttcgtcg aggacgagcc gcgcccattc cacgtcggcg atcgcgtcga
     2881 cgtctcggac cgcggtcccg taggtggtga tgacgacgtc ggcgccgtcg acctcggcgg
     2941 cgatctcgtc gggggaggcg cgattggtgc cgtggtgcac gacgactcgc aggccgggag
     3001 tgaaacgggc ggcttcggcg gcccagttgc cgacgacggc tgcgggagcg atgacgagcg
     3061 cgggcccttc ggccgcgccg gcgaggaggt gggcgagcat ggtcggtgtc ttgccgagac
     3121 ccatgtcgag ggcgagacag ccgccgagcc cggccgagtc gaggaagccg agccaggcca
     3181 gggcttcggc ttggtagtcg cggaggtcgc cgacgaatcc gtcgggggcg ccggccggtt
     3241 cggccgacag gttggcggcg gagctgagca gatcggcggc ccagccgccg cccgcgaccg
     3301 agatcccgcc cgagaggggc gagccctcga gaccgagagc gaggcggagc atttcggcgc
     3361 ctgacagctg ggtggttgcc gctcgttccg cgagcgtctc ggccgcggct tgcaggtcgg
     3421 cctggtcaat cgcgacccat cgtcccccgg agcggatcag gggttgggct tccttggcga
     3481 gtcgggcgat gtcgtccgcg gtgagctcga catcgtcgaa gagggccgac catcgcacgt
     3541 tggcgagctg gttggcgccg acggtggtgg tcgaggacgg gtcggcgaac acccgcagcg
     3601 tcggggaggg ccgacgccgc gagagggccg gtactcgcac atcgaagccg gcgccgctga
     3661 gccgaggacc ggtcgtcacc atcagctccc aggcctcgtc ctggctgagg atgacctggc
     3721 cgcggcgagt gctgccgggg cggagcagcg ccggcagcat ccgctcgagg cgcgtcagtt
     3781 cgtcttcgaa ggcgctgcgg tccgagccgg cgttgacgat cgcctgctcg atgggggtca
     3841 aggcgccgtc cggtccggga gcgagtaccg cgagatgcca ggcgccgcgg tcatcgggag
     3901 gatcgaggcg cacgacgagg ggcgcgtgtt ctcgcgtcac cgaccgggcc caccgctccc
     3961 cccgggctga gatctcgccg gcgattcgca ccggggcgtc gaaggcgctg ccgtcgagtc
     4021 gggcgaggaa tgcctcggcg acatcggcgg cggtgcgaac gcgtgggggt ggggcgggaa
     4081 cctcgaggcg acgagcgccg tcgcggcaga tcgcgccgac catcccggtg agcgcggagc
     4141 gggtcagggc ccgtcggtcg acgtgcgggt cgagggccag cacggtcccg ggcatggtgc
     4201 tggcgacctg ggtgaggcgg gtggggtcga tgagggccgg ggtccagcgc accgagtagg
     4261 agccgctgga ggcactggtg ctcccgtttc ctcggcggcg ttggcgcagc accggcacca
     4321 ccgcgccgcg tgccgttagc tccacggccc agatcgcgac gcggcccagc cagtgagcgc
     4381 tcggcccgag gtcgtcgccg gcttgtccgg ctccggctgc gaccagccat cccaagacct
     4441 caccaaccgg tgtggcgagc gcatcggcgg tgctggcgcc ggggagcacg acgacgccat
     4501 gcgggatcca acctgatgcc ggggcacccg cagcggtgag catggcgagg acctcgtcgc
     4561 cgtcggcgga cggggcgcca ggcccgcccg cccatgc
//
LOCUS       DATCCH010000443         6352 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_620347, whole genome shotgun sequence.
ACCESSION   DATCCH010000443 DATCCH010000000
VERSION     DATCCH010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6352
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620347"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="VIJ44_08540"
     CDS             complement(<1..656)
                     /locus_tag="VIJ44_08540"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165984.1"
                     /translation="MSLVHNVVGPPTAQRVLLLVHGYGADERDLGGLLPYLDPEGRFL
                     TVLPRGPVAAPPGFAWYDIGGITAGHREDVTFLDSLDQLDGLLDTVCEEHDRDRSEAI
                     VAGFSQGGGLAVALGLRRSERPHPAGVMALSSYLPDVEGVEYDWGATRQIPVLVQHGI
                     DDPLIPVERGRALATTLKEHELPVVYEEYPMGHAVALESIQAAKRWLDAVVAGERPSE
                     P"
     gene            711..1271
                     /locus_tag="VIJ44_08545"
     CDS             711..1271
                     /locus_tag="VIJ44_08545"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5165985.1"
                     /translation="MPLTVPALHDDMIVLRPPEAGDAAAITAAVQDPDIPRFTMVPSR
                     YTIDDAAAFIERSAGLWDSGDAAPFVIVDATTGTLLGSVGVHDLGLESGPAHVGYWVA
                     AAARGRGVATRALRLVARWTLEDLRLPRVEVYVFVENERSQRVATRAGFAREGIRAFV
                     GHPTGRGELVVLSKSGIAPLDTTARP"
     gene            1420..2355
                     /locus_tag="VIJ44_08550"
     CDS             1420..2355
                     /locus_tag="VIJ44_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165986.1"
                     /translation="MSGSRLEQHWVYPIVDGTGRRFSGPANAECRTRHTDYRRALLDG
                     WITRGEGPLESGFRSIREGDVLWLYAGVDAGVVGRAHVSKLAGRPEPWVTFTLDRPAS
                     RLLAQDPVPGNLVRRGFANMIDGPVPLADHPEACEGFQWWVDQLDDRDRRRLEPIGVS
                     SLRHTLTRQPSLLDPPALGALVRTLRSQDLAVGVATEPDGVARIVGLNGKLLVMGLAV
                     PGSQAAVPRDVLRSVGMLAWSGWSLAQRAPKLGLEPQLFLACKSAPSVELVRFLEDQH
                     HLVLWVHAAQVDFGPRTRLRWQSGLGATRPLARHG"
     gene            complement(2416..3231)
                     /locus_tag="VIJ44_08555"
     CDS             complement(2416..3231)
                     /locus_tag="VIJ44_08555"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HEY5165987.1"
                     /translation="MATDQELVDQLDQVWVSIGALGATLSEPEWKTPTEVPGWTVQDN
                     LAHIVALEWLGLGRPAPDHEIPDGLPHVKNDFGRLNEVFVDSRRDRTGEEALAEFRSV
                     TADRLAQLRAFGPDDFGADSFTPVGPGTVRDLLPFRVFDSWVHEQDMRRAVGRPGDLD
                     GPVADMAFGRVVASMPFVVGKKAQAPQGSTVCFDLTPPLAQRFAIRTAGRAQLLDAIP
                     TDVTVRIATDGETFVRLACGRLDPEKALADGAVDLDGDDAVGRRVVEALNFLF"
     gene            complement(3233..3964)
                     /locus_tag="VIJ44_08560"
     CDS             complement(3233..3964)
                     /locus_tag="VIJ44_08560"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF012697.4"
                     /note="catalyzes the formation of
                     (R)-4'-phosphopantothenate in coenzyme A biosynthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEY5165988.1"
                     /translation="MTDAADVVRTLVAARLPAARPLVLGIAGGVAAGKTRLADDVRDV
                     LTGVRAEVVATDGFLYPNAELTRRGLLARKGFPETYDTDQLRGFLTAVRAGELPQTVP
                     SYSHDTYDVRDAARTVEPLDVLIIEGINVLATAADLLDVGVYLDAADDDLEGWYRERF
                     VTLWAAGRDDPTSFYRSFADLDPDAVEALATRVCRSVNQVNLRDHIAPSRDLATCVIV
                     KGSDHRVLRVTMRDDDATSGPSTRS"
     gene            complement(3961..4395)
                     /locus_tag="VIJ44_08565"
     CDS             complement(3961..4395)
                     /locus_tag="VIJ44_08565"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY5165989.1"
                     /translation="MTSAAEAVRALVVAYAEQLDAGDLDGVAALFEHAVVRAARTGTE
                     LVGRDAVRAMYDPVIRYDDGTPHTQHVLANLEVRVDEDARTATSHCVFTVWQATRANG
                     LRPVLSGRYDDSFACVGAAWRFTERVIHADLLGDLSAHMRSR"
     gene            4494..5138
                     /locus_tag="VIJ44_08570"
     CDS             4494..5138
                     /locus_tag="VIJ44_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014374344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEY5165990.1"
                     /translation="MAIRAVIFDLGGVVMPSPLDVFRAYEARHGLPHRFLSEVVIETG
                     DHGAWSRFERGELDVEGFGVAFEAECAAAGGVVVVSELLGEIGTGTGPRPEMLAALHQ
                     IRVHGLATAALTNNWSTGEDRRMGSHYPEMVALFDVVVESAVEGLRKPDPRIYELACA
                     RLDVRPDQTVFLDDLGVNLKTARALGMTTIKVSEPAAALAELETILGFGLAPVS"
     gene            5138..5764
                     /locus_tag="VIJ44_08575"
     CDS             5138..5764
                     /locus_tag="VIJ44_08575"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY5165991.1"
                     /translation="MELILVRHAQPMRVEQGTVSGPADPGLSDTGHVQARRLAEWLAA
                     EPIDVVLTSPLCRARETAAPLAATLGVTAEVVDGVAEYDAAAGEYIPIEELRATKDER
                     WFATIEGRWADVGGVDPQEFQRRVVPAVDELIARFPGQRVVVVTHGGVLNVYLAHVVG
                     TEQLLWFHPEYTSISRVVAARSGQRSLATLNETAHLTARRVHIEEVAP"
     gene            5761..>6352
                     /locus_tag="VIJ44_08580"
     CDS             5761..>6352
                     /locus_tag="VIJ44_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020013936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEY5165992.1"
                     /translation="MTVHTERDGPVLIVTIDRPEARNAVDAPTAERLADAFRTFEGDD
                     DAAVAILTGAGGTFCAGADLKAMGDRRGNRVTADGDGPMGPTRMLTTKPVLAAVEGYA
                     VAGGLELACWCDLRVVARDAVFGVYCRRWGVPLIDGGTVRLPRLIGLSHALDLILTGR
                     GVSGDEAWRLGLANRLTEPGEALEIARGLDHDLAALP"
BASE COUNT          903 a         2251 c         2281 g          917 t
ORIGIN      
        1 ggctcgctgg ggcgctcccc ggcgacgacc gcgtcgagcc aacgcttggc cgcctgaatg
       61 ctttcgagcg ccaccgcgtg tcccatcggg tactcctcgt acaccaccgg gagttcgtgc
      121 tccttgagcg tcgtggcaag cgcccggccc cgctcgacgg ggatcaacgg gtcgtcgatg
      181 ccgtgctgca ccaacaccgg gatctgtcgc gtggcgcccc agtcgtactc gacgccttcg
      241 acatcgggga ggtacgacga cagggccatc accccggcgg gatggggccg ctcgctgcgc
      301 cgcaacccga gcgccaccgc gaggccgccc ccctgggaga agcccgcgac gatggcctcc
      361 gagcggtcgc ggtcatgttc ctcgcacacc gtgtcgagga ggccgtcgag ttggtccagc
      421 gaatcgagga acgtgacgtc ctcgcggtgt ccggcggtga tcccaccgat gtcgtaccag
      481 gcgaacccgg ggggcgcggc gaccggcccc cggggcaaca cggtgaggaa ccggccttcg
      541 gggtcgaggt acgggaggag cccgccgagg tcgcgctcgt cggctccgta tccgtggacg
      601 agcagcagca cccgctgcgc ggtcggcggt cccacgacgt tgtggacgag gctcatcggg
      661 ctcccgaaat ttcggcgttg tgtgcccgac gatgatggca cgatccatcg gtgcccctca
      721 ccgtcccggc gctccacgac gacatgatcg tcctccggcc tcccgaggcc ggtgacgcgg
      781 cggccatcac cgccgcggtg caggaccccg acatcccgcg gttcacgatg gtcccgtcgc
      841 ggtacaccat cgacgacgcg gcggcgttca tcgagcgcag cgccgggttg tgggactcgg
      901 gtgacgccgc cccgttcgtg atcgtcgacg cgacgaccgg gacactgctc ggatcagtcg
      961 gggtccacga cctcggcctc gaatccggtc ccgcgcacgt cgggtactgg gtcgcggcgg
     1021 cggcgcgggg ccggggggtc gccacccggg cgcttcgtct cgtcgcgcgg tggaccctcg
     1081 aggacctcag gctgcctcga gtggaggtgt acgtgttcgt cgagaacgag cggtcgcagc
     1141 gggtcgcgac tcgcgccggg ttcgcccggg aggggatccg cgccttcgtc gggcatccga
     1201 ccgggcgcgg cgagctcgtc gtgctctcga agtcgggcat cgccccgctc gacaccaccg
     1261 cccgaccctg aaccggggcg gggatggcga cgtgccgctc cgcgtgggcg aaggcgggga
     1321 ccaccgcgta gcattcgacc aggcagggtt caggtcggcc ccgtagggaa cgaccgaagg
     1381 ggcaaattca gcgatgagcc acggacagac cggcgccaga tgagcggctc gaggctggaa
     1441 cagcactggg tctacccgat cgtggacggc accggccgcc gattctcggg gcccgcgaac
     1501 gccgagtgcc gcacccggca caccgactac cgccgggcgc tcctcgacgg atggatcacc
     1561 cgcggtgagg ggcccctcga gtcggggttc cgctccatcc gggagggcga cgtcctgtgg
     1621 ctctacgccg gcgtcgacgc cggtgtcgtc ggccgggccc acgtcagcaa actcgcgggc
     1681 cgccccgagc catgggtgac gttcaccctg gaccggcccg ccagccggct gctggcccag
     1741 gaccccgtgc ccggcaacct ggtccgacga ggattcgcca acatgatcga cggcccggtc
     1801 ccgctcgccg accatcccga ggcgtgcgaa ggcttccaat ggtgggtcga ccagctcgac
     1861 gaccgcgacc ggcgccgcct cgaaccgatc ggcgtgtcgt cgttgcgcca caccctcacc
     1921 cgtcagccgt cactgctcga cccaccggcg ctcggcgctt tggtccgcac gctgcggtcc
     1981 caggatctcg ccgtgggcgt tgccaccgaa ccagacggcg tagcccgcat cgtcgggctc
     2041 aacggcaagc tgctggtcat gggcctggcg gtgcccggca gccaggccgc ggtcccccgc
     2101 gacgtgctcc gatccgtcgg aatgctcgcc tggtccggat ggtcgctcgc gcaacgcgca
     2161 ccgaagctgg ggctcgaacc gcagctgttc ttggcgtgca aatcggcgcc gagcgtcgaa
     2221 ctcgtccggt tcctcgagga ccaacaccac ctggtgctct gggttcacgc cgcgcaagtc
     2281 gacttcgggc ctcgcacccg gctgcgttgg cagtccggcc tgggcgcgac ccgtccgctg
     2341 gcgcgtcacg ggtagcccca ctcggcctcg atttcgtcag cctccgggca ccgagcctct
     2401 cggggcgccg gtgcgtcaga acaagaagtt cagcgcttcg acgacgcggc gaccgacggc
     2461 gtcgtcaccg tcgaggtcca cggcgccgtc ggcgagggcc ttctcggggt cgagacgtcc
     2521 gcaggcaagc cggacgaagg tctcaccgtc ggtggcgatg cggacggtca cgtcggtcgg
     2581 gatggcgtcg agcagctgcg cccgtccggc ggtgcggatc gcgaagcgct gagcgagtgg
     2641 cggcgtgagg tcgaagcaca cggtgctccc ctggggggct tgggccttct tgccgaccac
     2701 gaagggcatc gaggcgacga cacgtccgaa cgccatgtcg gcgaccgggc cgtcgaggtc
     2761 cccgggcctg ccgacggccc gacgcatgtc ctgctcgtgc acccaggagt cgaacactcg
     2821 gaacgggagc aggtctcgga cggtccccgg cccgaccggg gtgaacgagt cggcgccgaa
     2881 gtcgtcgggt ccgaaggccc gcagctgggc gagccggtcg gcggtcacgg accggaactc
     2941 ggcgagggcc tcctcgccgg tccggtcgcg gcgcgagtcg acgaacacct cgttgagacg
     3001 gccgaagtcg ttcttgacgt gcgggagacc gtcggggatc tcgtggtcgg gcgccggccg
     3061 gccgaggccc agccactcga gggcgacgat gtgcgcgagg ttgtcctgaa cggtccagcc
     3121 cggcacctcg gtcggtgtct tccactccgg ctcggagagg gtggcgccca gggcgccgat
     3181 cgaaacccag acctggtcga gctggtctac gagctcctgg tcggtcgcca tgtcagctcc
     3241 tggttgacgg tcccgaggtg gcgtcatcgt cgcgcatcgt cactcggagc acccggtggt
     3301 ccgagccttt cacgatcaca caggtggcga ggtctcggct cggcgcgatg tggtcgcgca
     3361 ggttcacctg gttgacactg cgacagaccc tggtggcgag cgcctcgacg gcgtcggggt
     3421 cgaggtcggc gaacgagcgg tagaaggacg tggggtcgtc gcggcccgcg gcccagaggg
     3481 tcacgaaccg ctcccggtac cacccctcga ggtcgtcgtc agcggcgtcg aggtacacac
     3541 cgacgtcgag caggtccgcg gccgtggcga ggacgttgat cccctcgatg atgagcacat
     3601 cgagcggttc gacggtgcgc gccgcgtcgc gcacgtcgta ggtgtcgtgg gagtagctcg
     3661 ggaccgtctg ggggagctcg ccggcgcgga ccgcggtgag gaagcctcgc agctggtcgg
     3721 tgtcgtaggt ctccgggaac ccctttcggg ccaggagccc gcggcgggtg agctcggcgt
     3781 tcgggtacaa gaagccgtcg gtggcgacga cctccgcccg caccccggtg agtacgtcac
     3841 ggacgtcatc cgccagccgg gtcttgcccg ccgcgacacc gccggcgatg cccagcacca
     3901 acgggcgtgc cgccgggagc cgcgccgcga cgagcgtgcg cacgacgtcg gccgcgtcgg
     3961 tcaccggctt cgcatgtgcg ccgagaggtc gccgagcagg tcggcgtgga tgacccgttc
     4021 agtgaaccgc caggcggccc cgacacacgc gaacgagtcg tcgtagcggc cggagaggac
     4081 gggtcgcagc ccgttggccc gcgtggcttg ccagaccgtg aagacacagt ggctcgtggc
     4141 ggtccgggcg tcctcgtcga cccggacctc gaggttcgcc aacacgtgct gggtgtgggg
     4201 ggtcccgtcg tcgtaacgga tcaccgggtc gtacatggcc cggaccgcgt cgcgaccgac
     4261 gagttcggtc cccgtgcgcg ccgcccgcac cacggcatgc tcgaacagcg ccgcgacacc
     4321 gtcgagatcg ccggcgtcga gctgttcggc gtacgccacc accagtgctc gcaccgcctc
     4381 ggccgctgac gtcatgggcg aatcatgccc cggcccgcgg cgtcgtgcgc cgtgcacaac
     4441 gcggcgccgc gggtgaccgg gacgcctgag gccacgagta gcgtccgcct cgcatggcga
     4501 tccgggcggt gatcttcgac ctcggcggcg tggtgatgcc gtcacccctc gacgtgttcc
     4561 gcgcctacga ggcccgccac gggctcccgc atcggttcct gagcgaggtt gtcatcgaga
     4621 ccggcgacca cggcgcctgg tcacggttcg agcgcggcga gctcgacgtc gagggattcg
     4681 gcgtcgcgtt cgaagcggaa tgcgccgcgg cgggcggcgt cgtcgtcgtg tcggagctgc
     4741 tgggcgagat tgggaccgga acgggtcctc gaccggagat gctcgcggcc ctccatcaga
     4801 tccgggtgca cggtctggcc acggcggcgc tgacgaacaa ctggagcacc ggcgaggacc
     4861 gccggatggg gtcgcattat cccgagatgg tggcgctgtt cgacgtcgtc gtggagtcgg
     4921 cagtcgaggg gctccgcaaa cccgaccccc gcatttacga gttggcctgc gcccgcctcg
     4981 acgtgcgacc agaccagacc gtgttcctcg acgacctcgg cgtgaacctc aagaccgccc
     5041 gggcgctcgg tatgacgacc atcaaggtga gcgaacccgc cgcggcactc gcggagctcg
     5101 agaccattct cggcttcggg ctcgcgcccg tcagctgatg gagctcatcc tggtccgtca
     5161 cgcccagccg atgcgcgtcg agcagggcac ggtgtccggt ccggccgacc ccggcctcag
     5221 cgacaccgga cacgtccaag cgcggcggct cgccgaatgg ctcgcggccg aaccgatcga
     5281 cgtcgtactg accagcccgc tgtgccgcgc ccgcgagacc gcggcgccgc tcgccgcgac
     5341 gctcggcgtg accgccgagg tcgtcgacgg cgtcgccgag tacgacgcgg cggcgggcga
     5401 gtacatcccc atcgaagagc tgcgggccac caaagacgaa cggtggttcg cgaccatcga
     5461 agggcgctgg gccgacgtcg gaggtgtcga cccccaggaa ttccaacgcc gggtcgtgcc
     5521 cgcggttgat gagctcatcg cgcgattccc cgggcagcgc gtcgtcgtgg tgacccacgg
     5581 cggtgtgctc aacgtctacc tcgcccacgt cgtcggcacc gagcagctgc tgtggttcca
     5641 tcccgagtac acgtcaatca gccgggtcgt cgcggcacgc tcgggacagc gcagcctcgc
     5701 gaccctcaac gagaccgcgc acctgaccgc gcgccgggtg cacatcgagg aggtcgcacc
     5761 gtgaccgtgc acaccgaacg ggacggaccg gtcctgatcg tcaccatcga tcggcccgag
     5821 gctcgcaacg cggtcgacgc cccgaccgcg gagcggctcg ccgacgcgtt ccgaaccttc
     5881 gagggcgatg acgacgctgc ggtggcgatc ctgaccggcg ccggcgggac gttctgcgcc
     5941 ggcgcggacc tcaaggcgat gggtgaccgc cgaggtaacc gggtcaccgc cgacggtgat
     6001 gggccgatgg gaccaacccg gatgctcacc accaagccgg tcctcgccgc ggtcgagggc
     6061 tacgccgtcg ccggcggcct cgagctggcc tgctggtgtg atctgcgcgt cgtggcccga
     6121 gacgcggtgt tcggtgtcta ctgccgacgc tggggcgtgc ccctcatcga cggcgggact
     6181 gtccggctcc cgcggctcat cgggctcagc cacgccctgg acctgatcct caccggccgc
     6241 ggcgtctcgg gggacgaggc gtggcgcctc ggtttggcca accggctcac cgaacccggg
     6301 gaggcgctcg agatcgcccg ggggctggac cacgacctcg ccgcgctccc cc
//
LOCUS       DATCCH010000444         2221 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_620358, whole genome shotgun sequence.
ACCESSION   DATCCH010000444 DATCCH010000000
VERSION     DATCCH010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620358"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="VIJ44_08585"
     CDS             complement(<1..422)
                     /locus_tag="VIJ44_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003927895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY5165993.1"
                     /translation="MNATVPIDAATGADDAAAIARGWIEDHVPVVWRRAAHDGGIEAI
                     RRVRTRGDYEQWYPEFGDSGLVVPTWPPEYGGLGVDATVAHAIDRVLAPYHLGRLNPL
                     GLNLCAPALFAHGTDAQRRRFLPPIVRNTERWCQLFSEP"
     gene            complement(419..1225)
                     /locus_tag="VIJ44_08590"
     CDS             complement(419..1225)
                     /locus_tag="VIJ44_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018637204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY5165994.1"
                     /translation="MTNAATPGLDAVLDGGTLRLTLDRPARRNALDDAMTEALIGHLE
                     HANVDEAVRVVVLAGRGDDFCAGFDLSTRGTATPERPRPGSIQRRLPAGAHRLIALLC
                     SVQVPVVAAVSGWAAGIGLHLALAADFCVAARDARLWEPFATRGFTPDSGGTWLLPRL
                     VGIARAKELLLLGRELSGSEAEAWGLIHTAVDGPDVTGRADELAATLAAGPTVALGLT
                     RWLVNTGGGLDLDRHLANEAFALELASRTADFREGLAALAERRRPDFGGR"
     gene            complement(1222..>2221)
                     /locus_tag="VIJ44_08595"
     CDS             complement(1222..>2221)
                     /locus_tag="VIJ44_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013426382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165995.1"
                     /translation="PAEHRDLMKGDPLRGVHAFYSASGREWWAPLGANRPVWRRNALV
                     GVLDKPSEFAGRAVHEWTGQVFRDDERLVSAQLRLMVRTEREAARQHDGGERRGPEPY
                     SDEDLAAIDEQYGQERRRGPEPRWWDDVEEGDELDPRVKGPLTVTDMICWHVGMGMGL
                     YGVRPYRLAFENRRRIPRFYHRDELNIPDVMQRVHWDPEFARRAGNPTTFDYGRMRET
                     WLIHVCTDWMGDDAWLWKLDCEFRRFNYVGDTHWMRGRVVRKYLAEGDRPAVDLELSG
                     ENQRHELTTPGHATVLLPSRSHGPVRLPDPPGGATSLEAALGAIAARYREPAGSRR"
BASE COUNT          299 a          792 c          817 g          313 t
ORIGIN      
        1 ggctcgctga acagctggca ccagcgttcg gtgttgcgaa cgatgggagg aaggaaccgt
       61 cgccgttgcg cgtcggtgcc gtgcgcgaac agcgccggtg cgcacaggtt caggcccagc
      121 gggttcaggc ggccgaggtg atacggcgcc agcacccgat cgatggcgtg ggcgacggtg
      181 gcgtcgacac cgaggccgcc gtactcgggc ggccacgtcg ggaccacaag tccggagtcg
      241 ccgaactcgg ggtaccactg ctcgtagtcg ccgcgggtgc gcacccgtcg gatggcttcg
      301 ataccgccgt cgtgggcggc gcggcgccac acgaccggca cgtggtcctc gatccacccg
      361 cgggcgatcg ccgcggcgtc gtcggcaccg gtggccgcat cgattggcac cgtggcgttc
      421 atcggccccc gaagtcgggg cggcgccgct ccgcgagcgc ggcgaggcct tcccggaaat
      481 cggcggtgcg cgaggcgagc tcgagcgcga acgcctcgtt ggcgaggtgg cggtcgaggt
      541 cgaggccacc cccggtgttc acgagccagc gggtgagccc gagcgcgacg gtcggcccgg
      601 cggcgagcgt ggccgcgagc tcgtcggccc ggcccgtcac gtcgggtccg tcgaccgcgg
      661 tgtggatcaa cccccacgct tcggcctcgc tccccgacag ctcccggccg agcaggagga
      721 gctccttggc gcgagcgatc ccgaccagcc gggggagcag ccacgtgccg ccactgtcgg
      781 gcgtgaagcc ccgggtcgcg aacggctccc acagccgggc gtcgcgcgct gcgacgcaga
      841 agtcggcggc gagcgccagg tgcaggccga tgcccgcggc ccaccccgac acggcggcga
      901 cgaccggtac ctgcaccgag cagaggaggg cgatgaggcg gtgcgccccg gccgggaggc
      961 gacgttggat gctcccggga cggggccgct ccggggtggc ggtgccgcgg gtcgagaggt
     1021 cgaacccggc gcagaaatcg tcgccgcggc cggcgaggac gacgactcgg accgcttcgt
     1081 ccacgttggc gtgctccagg tgaccgatca gggcctcggt catcgcgtcg tccagggcgt
     1141 tgcggcgggc cgggcggtcg agcgtgagac ggagcgtccc gccgtcgagc acggcgtcga
     1201 ggcccggcgt ggcggcgttg gtcatcgacg gctcccggcc ggttcgcggt accgggccgc
     1261 gatggccccg agcgcggcct caaggctggt cgcgccccca ggcggatccg ggagccgcac
     1321 cggcccatgg gaccggctgg ggagcaggac ggtggcgtgg cccggcgtgg tcagctcgtg
     1381 gcgctgattc tctccggaca actcgaggtc gaccgcgggt cgatcgccct ccgcgaggta
     1441 cttgcgcacg acgcggcccc gcatccaatg ggtgtcgccc acgtagttga accgtcggaa
     1501 ctcgcagtcg agtttccaga gccaggcgtc gtcgcccatc cagtcggtgc acacgtggat
     1561 caaccaggtc tcgcgcatgc ggccgtagtc gaaggtcgtc gggttcccgg ctcggcgcgc
     1621 gaactccgga tcccagtgca cccgttgcat cacgtcgggg atgttcaact cgtcgcgatg
     1681 gtagaagcgc gggatgcgcc gccggttctc gaacgccagc cggtacgggc gcaccccata
     1741 cagccccatg cccatcccga catgccagca gatcatgtcg gtcacggtca ggggaccctt
     1801 cacgcgtggg tcgagctcat cgccttcctc gacgtcgtcc caccagcggg gctcggggcc
     1861 gcgccggcgt tcttgcccgt actgctcgtc gatcgccgcc aagtcctcgt cgctgtacgg
     1921 ctccggtccc cgccgttcgc cgccgtcgtg ttgacgagcg gcctcccgtt cggtgcggac
     1981 catgagccgc agctgagccg acaccaaccg ctcgtcgtcc cggaacactt gtcccgtcca
     2041 ctcgtgcaca gcgcggccgg cgaactcgct cggcttgtcg agtacaccga cgagcgcatt
     2101 gcgccgccag accggccggt tcgccccgag tggcgcccac cactctcgtc cgctcgccga
     2161 gtagaaggcg tgcaccccgc gcagtgggtc gcccttcatg aggtcgcggt gctcggcggg
     2221 g
//
LOCUS       DATCCH010000445         4268 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_404802, whole genome shotgun sequence.
ACCESSION   DATCCH010000445 DATCCH010000000
VERSION     DATCCH010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4268
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404802"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2088)
                     /locus_tag="VIJ44_08600"
     CDS             complement(<1..2088)
                     /locus_tag="VIJ44_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5165996.1"
                     /translation="MAATQTSGVRPRAGTYAVLFTLTLATLVYEIALTRIFSVVMWYH
                     FAFVAISVALFGLTIGALIVHHFPDRFPAPAARRQLGWCALLFSASIVPCLLIQLSIK
                     FIPELNLAGLWSVLMTCSVVSIPFVFSGIAVTIALTRFPRRVNRLYAADLVGAAVGCA
                     LVVWLLNRLDGPSVIIVVGALGAVGALFFVLDASIKWAPWLAAFLAVALATLAVVNGY
                     MSKDGNALVKVNWVKGQRDGSHLNVTWNAFSRLTVDGHPSAPTGITIDGTAGTALLPF
                     DGNPASASWRRDDFTSLVHYVRPNADTAVIGSGGGADVLSSIAFRDRSTTGIEINPGI
                     LHLANGVYGNYTGHLDRVPNVRFVNDEARSYLTRNNKRFDIIQISLIDTWAAESAGAY
                     ALSENSLYTTHAWQVYFDRLKTGGVLSVTRFYTFPGSGKPLEVYRTAALAAQVLHDRG
                     VKNPRAHLLIYHGPRLSVGVELGTVLVSPHGFTQQELATVAATASRLGFTPVLTPTVV
                     TDPRFAALASPGGPGPGVRGIAEDISPPTDDHPFFFQMANLHTFLSGSGLQDNFATRP
                     VFVLLLLAIAIVLLALGFILLPLLVTTKRGDHRGMGPFYLYFSGIGLGFLLVELSQLQ
                     RLSIFLGNPTSGLTVVLFSVLLFSGLGSMLTERFVRIDNPRSVLVPLLVLLAVIVAFG
                     AATPLIIRQFESAT"
     gene            2269..3042
                     /locus_tag="VIJ44_08605"
     CDS             2269..3042
                     /locus_tag="VIJ44_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010477123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HEY5165997.1"
                     /translation="MTTDAVHRTFRERAVDVANRVGERFLRWLDRYFGRHSLVGDGTF
                     FDPADFPWVKRVEAGFPQIRAELDEVLRYHADLPNFQDISTDQYQLTNDDRWKTFFFY
                     GYGFKAGNNVDRCPDTDRLLGEIPGMTTAMFSILSPHKKIPPHGGPYKGVLRYHLGIL
                     VPDPVDGCGIRVGHDIRQWREGQSLVFDDTFEHEAWNDTDGVRVVLFVDFKRPLRGPA
                     RWINETVLKVIGVSPFIQDAKARHNAWERRFEEIRNRGA"
     gene            complement(3070..>4268)
                     /locus_tag="VIJ44_08610"
     CDS             complement(3070..>4268)
                     /locus_tag="VIJ44_08610"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY5165998.1"
                     /translation="DRLHAFPRYELYQLGVDTGLYPYYRSVESASRPRVTVEGREVIN
                     FGSNNYLSLSYHPRVIDAAVNATRRFGSGVTGSRLLNGTLTLHRELESELADFYGLPS
                     CLVFSTGYVANASAIAGFLGRHDVAIVDKEAHNSLLSGVRLSGAAMRRFRHNDVAHLE
                     KVLAGLPAEVGKGVIVDGVYSMSGDTAPLAEMVEICRRYPNTFLLDDEAHGLGVLGER
                     GRGALEQQGVLNDVDLVTVTFSKSLGSCGGALLGPSDAIELLTVTSDLFIFTASNTPG
                     SVAAALEALRILREEPERPARLQANVDLFVGLLAERGVPTNPAQSAIVTIPLRRADDA
                     SVAMVFRDLFEGGVFVNPVISPAVTSEAGLIRLSLMVEHDAAMLEEASELLYKVIAEH
                     DQFAAR"
BASE COUNT          805 a         1342 c         1469 g          652 t
ORIGIN      
        1 cgtcgccgac tcgaactggc ggatgattaa gggcgtggca gcaccgaacg ccacgatcac
       61 cgcgagcagc accagcagcg gcacgagcac agaacgcggg ttgtcgatcc ggacgaaccg
      121 ttcggtcagc atgctgccga gaccgctaaa gagcagcacg gagaacaaga ccacggtaag
      181 ccccgaggtc gggttgccga ggaagatgct cagtcgctgc agctgcgaca actcgaccag
      241 caagaaaccc aacccgattc ctgagaagta caggtagaag ggacccattc cccgatgatc
      301 gccacgtttg gtggtcacca gcagcgggag gaggatgaaa ccgagagcca atagcacgat
      361 cgcgatcgcc aagagcaaca ggacgaagac gggccgggtg gcgaagttgt cctggagtcc
      421 cgagccgctg aggaacgtgt gcagattcgc catctggaag aagaagggat ggtcgtccgt
      481 cggcggcgag atgtcctcgg cgatgccgcg cacgccaggg cccggccctc ccgggctggc
      541 gagggcagcg aagcgagggt cggtgaccac ggttggcgtc aagaccggcg tgaagccgag
      601 tcgtgatgcc gtcgccgcca ccgttgcgag ctcctgctgc gtgaaaccgt ggggactgac
      661 gagcacggtc cccaactcca cccccaccga caggcgggga ccgtggtaga tcagcagatg
      721 ggcgcgtggg ttcttcaccc cccggtcatg gagtacctga gccgcgagcg cggcggtgcg
      781 gtacacctcg agcggcttac ccgaaccggg gaaggtgtag aaccgggtga cggagagcac
      841 cccgccggtc ttcaaccggt cgaagtagac ctgccatgcg tgcgtcgtgt agagcgagtt
      901 ctcgctgagg gcatacgcgc ccgcggattc agcagcccag gtgtcgataa gtgagatctg
      961 gatgatgtcg aagcgcttgt tgttacgcgt gaggtagctc cgagcctcgt cgttcacgaa
     1021 ccggacgttc gggacccggt cgagatgacc ggtgtagttg ccgtagacgc cattggcgag
     1081 atgaaggatt ccggggttga tctcgatacc agtggtggac cggtcacgga acgcgatcga
     1141 agacagcacg tccgcgccgc cgcccgagcc gatgacggca gtgtcggcgt tcggccggac
     1201 gtagtgcacc agactggtga agtcgtcgcg acgccaggac gccgaagccg ggttgccgtc
     1261 gaacggaagc agggcagtcc ccgcggttcc gtcgatggtg atcccggtcg gagccgacgg
     1321 atgtccgtcg accgtaaggc gggagaaggc gttccacgtc acgttgaggt gcgacccgtc
     1381 ccgctgtccc ttcacccagt tgaccttgac gagcgcgttg ccgtctttgg acatgtatcc
     1441 gtttacgacc gcgagcgtcg ccagcgcgac ggcgaggaac gcggcgagcc acggggccca
     1501 cttgatcgat gcgtccagca caaagaacaa ggcgccgacc gcgcccagag cgccgaccac
     1561 gatgatgacg ctgggcccgt caagccggtt gagcagccag acgactaagg cacagccgac
     1621 cgcggcgccg acgaggtccg cggcgtacag gcggttgacg cgtctcggga atctcgtcag
     1681 ggcgatcgtg acggcgattc cgctgaagac gaacggtatc gacacgaccg agcacgtcat
     1741 caggaccgac cagagaccgg ccagattcag ttccgggatg aacttgatcg agagttggat
     1801 cagcaggcac gggacgatcg aggcactaaa gagaagcgcg caccagccca attggcgtcg
     1861 tgcggccgga gccgggaaac ggtccgggaa atggtggacg atgagcgcac cgatcgtgag
     1921 cccgaacagc gccaccgaga tcgcgacgaa cgcgaagtga taccacatga ccacgctgaa
     1981 gattcgggtg agcgcgatct cgtacacgag tgtcgcgagc gtcagggtga agaggaccgc
     2041 ataggtgccg gcgcgcgggc ggacccccga ggtttgtgtt gcagccatga ccctcggctc
     2101 cccccccacc gacgtcggac gaggcagcct gtccgagccg gtgatactaa cgacatccgc
     2161 gagaaccccg ggcgtcggcg tgaggccggg tcggcaccga tcatgccggg ttccggtcgc
     2221 ggtgctgacg tgttgacaga tcccgagggc gagtaaccta acgaggtcgt gacgaccgac
     2281 gccgtgcatc gcaccttccg ggaacgggcc gtcgacgtcg cgaaccgggt aggggagcgg
     2341 ttcttacgct ggctcgaccg ctacttcggg cgccattcgc tggtcgggga cggcaccttc
     2401 ttcgatcctg cggacttccc gtgggtgaag cgggtggagg ccgggttccc gcagatccgc
     2461 gccgagctcg acgaggtgct gcgctaccac gccgatctcc caaacttcca ggacatctcc
     2521 accgaccagt accagctgac caacgacgac cgttggaaga ccttcttctt ttacgggtac
     2581 ggcttcaagg ccggcaacaa cgtcgaccgg tgccccgaca ccgaccggct cctaggcgag
     2641 atcccgggca tgacgaccgc gatgttctcg atcctctcgc cccacaagaa gatcccgccg
     2701 cacggcggcc cgtacaaagg ggtgttgcgt taccacctcg ggatcctcgt gcccgacccc
     2761 gtcgacgggt gcgggatccg ggtcggtcac gacatccgcc agtggcgcga aggtcagagc
     2821 ctggtcttcg acgacacctt cgagcacgag gcgtggaacg acaccgacgg ggtccgggtt
     2881 gtcctcttcg tcgacttcaa gcggccgttg cgcggcccgg cccgctggat caacgagacg
     2941 gtgctcaagg tgatcggcgt gtcaccgttc attcaggacg ccaaggctcg tcacaacgcc
     3001 tgggagcgac gcttcgagga gatccgcaac cgcggcgcct gaccgccggc gccgaccggt
     3061 ggcccccggc tagcgggcgg cgaactggtc gtgttcggcg atcaccttgt agagcagctc
     3121 ggaggcctct tcgagcatcg cggcgtcgtg ttccaccatg aggctcaaac ggatgaggcc
     3181 ggcctcgctg gtcaccgcgg gtgagatcac cgggttcacg aacacgccac cttcgaagag
     3241 gtcccggaac accatcgcga ccgaggcgtc atcggcgcgc cgcaacggga tggtgacgat
     3301 cgcgctttgg gccgggttcg tggggacgcc gcgctcggcg agcaacccga cgaagaggtc
     3361 cacgttggct tgcagacgag ccggccgttc gggctcctcg cgcaagatgc ggagcgcctc
     3421 gagtgccgcg gcgaccgaac cgggagtgtt cgacgcggtg aagatgaaca ggtccgacgt
     3481 gaccgtgagc agctcgatcg cgtcgctcgg gccgagcagc gcgccgccgc agcttccgag
     3541 cgacttggag aacgtgacgg tcacgagatc gacgtcgttg aggacgccct gttgctccag
     3601 cgccccccgt ccccgttcgc cgaggacgcc gaggccgtgg gcttcgtcgt cgagcaggaa
     3661 cgtgttgggg tatcgacgac agatctcgac catctcggcg agcggtgcgg tgtcgccact
     3721 catcgagtag acgccgtcga cgatgacgcc tttcccgacc tcggccggca ggccggccag
     3781 caccttctcg agatgggcga cgtcgttgtg tcggaagcga cgcatcgccg cccccgagag
     3841 gcgcacgccg ctcagcagcg agttgtgggc ctccttgtcg acgatggcga cgtcgtggcg
     3901 gcctaggaac ccggcgatgg cgctggcgtt cgccacgtag ccggtggaga acacgagaca
     3961 cgacggcagc ccgtagaagt cggcgagctc actttcgagc tcgcggtgca gggtcagcgt
     4021 cccgttgagc agtcgggacc cggtgacccc gctcccgaac cggcgggtcg cattcaccgc
     4081 cgcgtcgatg acccgagggt gatacgagag gctcagatag ttgttcgacc cgaagttgat
     4141 gacctcgcgg ccctcgacgg tgactcgggg ccgggaggcg ctctccaccg accggtagta
     4201 cgggtagagg ccggtgtcca cgccgagctg gtagagctcg tatcgcggga aggcgtgcag
     4261 tcgatcgg
//
LOCUS       DATCCH010000446         2941 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_454297, whole genome shotgun sequence.
ACCESSION   DATCCH010000446 DATCCH010000000
VERSION     DATCCH010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454297"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1205
                     /locus_tag="VIJ44_08615"
     CDS             <1..1205
                     /locus_tag="VIJ44_08615"
                     /inference="COORDINATES: protein motif:HMM:NF018938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HEY5165999.1"
                     /translation="GYTKDLPAYLRAALPAVAAGRTKIITNAGGINAASAARAAIDTA
                     RELGLSGLTVATVLGDDLMPRLDELAGATGLTHLESGAPFGAFPTPALFASAYLGARP
                     IVEALAAGADVVITGRVADAALFLAPLVHEHGWAWDDWDRLAAGTLVGHLLECSGQSA
                     GGNLSRDWWTIPRPWDLPYPIAEVEADGSALITKPESSGGRVSFDTVRHQLLYEVHDP
                     AAYLAPDVVCDFTSAEFSDEGDDRVRVTGVRGQPATSTYKALLAHHAGWAGETRVAFS
                     WPDAREKATATAAIFAERVRLAGLAVAEWCFELWGVDALGGPTVPPTTAEAPEVVLRA
                     AWRCDDARTTALVGREMVPLTLSGPAAGMTGMGRGGASPSELLGLWPTLIDKDLVDPR
                     VTVAVETV"
     gene            1205..1597
                     /locus_tag="VIJ44_08620"
     CDS             1205..1597
                     /locus_tag="VIJ44_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5166000.1"
                     /translation="MTSCRLHDLGCGYRSGDKGDISDIALFADDTEVYELIRAAVTAE
                     RVKAHFAGLVHGPVERYEAPNVLALNFVLHDALGGGAPRSLRADNLGKTLGGALVRLE
                     IEVPEHLAHRRALTPDVRWALTILDRLS"
     gene            complement(1547..>2941)
                     /locus_tag="VIJ44_08625"
     CDS             complement(1547..>2941)
                     /locus_tag="VIJ44_08625"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY5166001.1"
                     /translation="WSLAAIAAERDRSQTRLSHQALHDTLTGLPNRQQALERLRRIDR
                     HGRQGGPDVAVLFLDLDRFKVLNDSVGHDAGDRLLVEMGRRLQEALRPGDLVTRFGGD
                     EFVIVCEQLDGVEEAHALAERLLAVAREPLDLDGLETVVTASIGIALADGRPPAALLR
                     DADAAMYRAKEKGRDRAELFDAQLRDDVIARLDTERELRHALDHGGLLLYYQPIVSLQ
                     TGAVTGLESLLRWDHPTRGLLTPTDFLGVAEESGLMRPIGEWIRAEVCRTAARWHQEH
                     PEWVRLVTSVNVSAAELSDPHLATNIAKTVLDSDLDPSLLSFEITERVLFQDVEAARG
                     LFNELHELGVQLALDDFGTGYSPLVHLKHFAIDSVKIDQVFVAGLGSDPFDDAIVDAV
                     VDLSNQLDLVSVAAGVETAAQEERLHAIGCRQAQGHRLAPPMPGPLAEAWLARRIPLA
                     ESVEDGQRPADIGG"
BASE COUNT          429 a         1022 c         1054 g          436 t
ORIGIN      
        1 gcggctacac caaggacctg cccgcctacc tgcgcgccgc gttgccggcc gtcgcagcgg
       61 gccgcacgaa aatcatcacg aacgcgggtg gcatcaatgc cgccagcgcg gcgcgagccg
      121 cgatcgacac tgctcgggag ctcggcctga gcgggttgac cgtcgccacc gtcttgggcg
      181 acgacctgat gccgcgcctc gacgagctgg cgggcgctac gggactcacc cacctcgaat
      241 cgggggcccc gttcggggcg ttccccacgc cggcgctgtt cgcgtcggcg tacctcggag
      301 ctcgccccat cgtggaggcc ctcgccgcgg gagccgacgt cgtgatcacc ggtcgcgtcg
      361 ccgacgccgc cttgttcctc gcgcccctcg tccacgagca cggttgggcg tgggacgact
      421 gggatcgcct cgcggcgggg acgctcgtcg ggcatctcct cgagtgctca ggccagagcg
      481 ccggcgggaa cctcagtcgg gactggtgga ccatcccccg gccgtgggat ctgccgtacc
      541 cgatcgcgga ggtcgaggct gacggcagcg cgttgatcac caagcccgag tcctcagggg
      601 gacgggtgag cttcgacacc gtccgccatc agctcctcta tgaggtacac gaccccgcgg
      661 cgtacctcgc cccggacgtg gtctgtgact tcaccagcgc cgagttcagc gacgagggcg
      721 acgaccgggt ccgggtgacc ggcgtgcgcg gacagccggc aacctcgacc tacaaggcgc
      781 tcctcgcgca tcacgccggg tgggccggcg agacgagagt cgcgttctcc tggcccgacg
      841 cccgcgagaa ggcgaccgcg accgcggcga tcttcgccga acgcgtccgc ctggccgggc
      901 tggcggtggc cgaatggtgc ttcgagctct ggggtgtcga cgcgctcggc ggcccgaccg
      961 tcccgcccac gaccgccgag gcccccgagg tggtgctgcg cgccgcctgg cggtgcgacg
     1021 acgcccgcac gaccgcgctc gtgggacggg agatggtgcc cctcaccctg tcggggccgg
     1081 ccgccggcat gaccgggatg ggacggggcg gcgcgagccc gagcgagctg ctggggctct
     1141 ggccgacgct gatcgacaaa gaccttgtcg acccccgcgt caccgtggcc gtggagacgg
     1201 tgtgatgacg tcgtgtcggt tgcacgacct cggctgtggc taccgctccg gcgacaaggg
     1261 cgacatctcg gacatcgcgt tgttcgccga cgacaccgag gtttacgagc tgatccgcgc
     1321 cgcggtgacg gccgaacgag tcaaggctca tttcgccggt ctcgtgcacg gccccgtcga
     1381 acggtatgag gcgccgaatg tcctggcgtt gaacttcgtc ctccacgacg cgctcggcgg
     1441 aggggcacca cggtcgctgc gcgccgacaa cctcggcaag accctgggcg gcgcgttggt
     1501 gcgcctcgag atcgaggtcc ccgagcatct cgcacaccgt cgggcgctaa cccccgatgt
     1561 ccgctgggcg ctgaccatcc tcgaccgact cagctagcgg gatgcgtcgg gcgagccagg
     1621 cctcggcgag cgggccgggc atcggcggcg cgaggcggtg gccttgggcc tgccggcacc
     1681 cgatggcgtg gagccgttcc tcttgggcgg cggtctccac acccgcggcg accgacacca
     1741 gatcgagttg attcgacagg tcgacgacgg cgtcgacgat cgcgtcgtcg aagggatccg
     1801 accccagccc ggcgacgaag acctgatcga tcttgaccga gtcgatcgcg aagtgcttga
     1861 gatgcaccag cggcgagtac ccggtcccga agtcgtcgag ggctagttgg accccgagct
     1921 cgtgcagctc gttgaacagc ccccgggcgg cttcgacgtc ttggaacagg acccgctcgg
     1981 tgatctcgaa cgacagcagc gacgggtcga gatctgagtc caagaccgtc ttggcgatgt
     2041 tcgtcgccag gtgcggatcg gagagctcgg ccgcggacac gttgacgctc gtgacgagac
     2101 ggacccattc cgggtgctcc tggtgccatc gcgccgcggt gcggcacacc tccgcccgga
     2161 tccactcccc gatcgggcgc atgaggcccg attcctcggc cacgccgagg aagtcggtcg
     2221 gggtgagcaa ccctcgagtc ggatgatccc agcgcagcaa cgactcgagg cccgtcaccg
     2281 ctccggtctg cagcgacacg atcggctggt agtagaggag cagcccgccg tggtcgagcg
     2341 cgtgccgcag ctcgcgttcg gtatcgagcc tggctatgac gtcgtcgcga agctgcgcgt
     2401 cgaacagctc ggcccggtca cggcctttct ccttcgcccg gtacatggcg gcgtcggcgt
     2461 cgcgaaggag cgccgcgggg ggccggccgt cggcgagggc gatcccgatg ctggcggtga
     2521 cgacggtctc gagcccatcg aggtcgagcg gctcgcgggc aacggccagg agccgttcag
     2581 cgagggcgtg ggcctcctcg accccgtcga gctgctcgca cacgatgacg aactcgtcgc
     2641 cgccgaaccg ggtcacgaga tcgccgggac gcaacgcctc ctggaggcgc cgacccatct
     2701 cgacgagaag tcggtcgccg gcgtcatggc ccacgctgtc gttgaggacc ttgaaccggt
     2761 cgaggtcgag gaagagcacc gcgacatcgg gcccgccttg ccgtccgtgc cgatcgatcc
     2821 gccgcagccg ttcgagggcc tgctgccggt tggggagacc ggtgagggtg tcgtgcaagg
     2881 cctgatggga gaggcgcgtc tggctccggt cgcgttcggc ggcgatggcg gcgaggctcc
     2941 a
//
LOCUS       DATCCH010000447         6431 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_246148, whole genome shotgun sequence.
ACCESSION   DATCCH010000447 DATCCH010000000
VERSION     DATCCH010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6431
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246148"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="VIJ44_08630"
     CDS             <1..655
                     /locus_tag="VIJ44_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY5166002.1"
                     /translation="PSKIICLGLNYEPHIREMGHDPPDVPTLFAKYAAALVGPRDPIV
                     LPHVSTSVDWEAELAFVIGSPVRHADEPTARAAIAGYTICNDVSMRDWQRRTPQWLQG
                     KTFESSTPVGPVLVTPDEVDDARDLRVRCEVDGELRQDARTSELVFDPVAIVQYVSMI
                     LTLLPGDLISTGTPGGVGAGMSPPVFLRPGQVVRTVIDGIGELVNECVAETDPRTTP"
     gene            complement(670..1485)
                     /locus_tag="VIJ44_08635"
     CDS             complement(670..1485)
                     /locus_tag="VIJ44_08635"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5166003.1"
                     /translation="MTVEARAALPPGRVVADIGLIAGRNLRKVLRSPGLIVFSTIQPL
                     MQLVLFAFVFGAIATVGRGTSYKDFVVPAVLVQAITFSAMGSGVGIAYDLQSGMVDRF
                     RSLPIARSAFLVGRTTSDSLRLGIQSVLLVGASLAIGFSFHNGVLAAVGMVVVVVLFG
                     MALTAFSAWIGLAVKDPETVQPAVFIPVLPLVFTSSAFAPVSRLPGWMQLVARWNPIT
                     SAIDTARGLALGDKTLFAVSHTHLTTAAGHFAIWWVVIVSVFTALAVHRYRVG"
     gene            complement(1515..2519)
                     /locus_tag="VIJ44_08640"
     CDS             complement(1515..2519)
                     /locus_tag="VIJ44_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5166004.1"
                     /translation="MDSTGVVVEGLIKRFGSFVALGGVDFVVPEGTVVGLLGPNGAGK
                     TTVIRILSTLLRPDGGRARVAGYDIVREPQHVRAVIGLTGQFAAVDEDLTGRENLVLV
                     GRLGRLSKVVAQQRAEALLDAFDLADAADRVLRGYSGGMRRRLDLGASLIATPAVLFL
                     DEPTTGLDPRSRLRLWDVITDLRDEGTTIVLTTQYMEEADQLAQNISVIDGGRIIAEG
                     TADQLKTKVGGDVLDLRVGDRARVGDAAATLATAFSVPKAEITIDAELGTVLVPVQSG
                     AAALVDAVRALDAAGVPVADLGLRRPSLDDVFLSLTGHHAEEPRPDAPEPRARRRVRA
                     "
     gene            2674..3192
                     /locus_tag="VIJ44_08645"
     CDS             2674..3192
                     /locus_tag="VIJ44_08645"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY5166005.1"
                     /translation="MRDRFDGCLNEHGASLTTWAVLRSTEHEDGLSQRELASRMSIES
                     PTLVRHLDRLEDEGLVVRRRDAQDRRVVRVGLTPAGHRRYAELRDVAANVDSQLRSLL
                     DDDEIATLERAPPIPSPDPGDQLRRDLGGEPLVARARPSGHRGSRPQAGGSPGVGPGA
                     PGRPEHRGARDR"
     gene            3227..4543
                     /locus_tag="VIJ44_08650"
     CDS             3227..4543
                     /locus_tag="VIJ44_08650"
                     /inference="COORDINATES: protein motif:HMM:NF015715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEY5166006.1"
                     /translation="MGVLEDVGRSLREGFFMFWETLWPLILGFALSGAVQAFVSRDQM
                     ERVMGDHRPRTVTRASAIGMASSSCSYAATAMAKSLFEKGADFTTALIFMFASTNLVL
                     ELGIVLVVLLGWQFALAEFVGGPIMIALLALTGSFVVGKSIAARARRNLRAAREADDP
                     DAMAGVSEERQAELEREPWRKKLRSRAAWSDAATYTVADLTMLRRELAVGYLVAGFLT
                     VLVPVQVWNDVFIHGHGWWTSVENVVVGPFVAIISFVCSIGNVPLAAALWHGGISFGG
                     VIAFIFADLITFPLLLIYRRYYGWRVTLRLLLWFWAVMAVAGLAVEGLFDVTGLIPRT
                     RPRDIVPTTFQWNYTTFLNLAFLVVAGYLYWLYRNRDRFGGGAGYAIDPVCGMQVQTA
                     NAPASTHHDGRSYWFCSDRCADQFRRDPDVHTPAAGPVSNPAPGPS"
     gene            complement(4580..>6431)
                     /locus_tag="VIJ44_08655"
     CDS             complement(4580..>6431)
                     /locus_tag="VIJ44_08655"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HEY5166007.1"
                     /translation="DLEAHGIESTWETRERVVVALTGAPGADTLIRRAARMARRTKGD
                     LIGVHVRPTDGLASRTSDLLVSHRDLLADLGGAYHEVGGDDVGEALVQFARSERATQL
                     VVGASRRRRWTELVHGSVISRILRWADTIDVHVIATETPDPDREVGSPIRVVRSGISR
                     RRQGIACVLAVLALPLLTVALTAVRSDVSLPSDLLFYLLVVIGVAAVGGLVPAVLSAV
                     AGFLLSKWFFTQPFHTLNVDRDDNLVALIVFVVVAVVVGELVSRVARRRAQASRAQAD
                     AETLAALSGTLLSDHDPVPALMAGLRDAFGCQSVSVLRRSGDGWRVEVSAGDPVPQTP
                     DDAQVSVSLDADTTLAVCGEGIGEEDLQMLRAFVGQLALAVERRRLRAEAAAAEGLAE
                     ANELRTALLAAVSHDLRTPLAAIKAAVTSLLQPDVTLDAGSKNELLDAIDEETDRLTK
                     LVGNLLDMSRLQTGSFELVWRDVGLEEVVAAALQSLGDTTGRVVVDVPETLPRVRADA
                     ALLERAIANVVANALQASPPDRPVRIFAGASAERVELTIVDSGPGIPRSKREEVFQPF
                     QRLGDNPQGTGVGLGLAVARGFVEAMDGELSLDDTPGGGATVAISVPVTR"
BASE COUNT          990 a         2300 c         2211 g          930 t
ORIGIN      
        1 cccgtcgaag atcatctgcc tggggctcaa ctacgagccc cacatccgcg agatgggcca
       61 tgacccaccc gacgtcccga cgctgttcgc caagtacgcg gcggcgctcg tcggcccccg
      121 cgacccgatc gtcttgccgc acgtgtccac cagcgtggac tgggaagccg agctggcgtt
      181 cgtgatcggc tcaccggtgc gccacgccga cgagccgacg gcccgggccg cgatcgcggg
      241 gtacacgatc tgcaacgacg tgtcgatgcg cgactggcag cgccgcacgc cgcagtggct
      301 gcagggcaag acgttcgaat cgtcgacacc ggtcgggccg gtcctggtca cccccgatga
      361 ggtcgacgac gcccgcgacc tccgggtgcg ctgcgaggtc gacggtgaac tgcgccagga
      421 cgcccgcacc tcggagttgg tattcgatcc ggtcgcgatc gtccagtacg tgagcatgat
      481 cctgaccttg ctgcccggcg acctcatctc cacgggaacg cccgggggcg tcggcgccgg
      541 gatgagccca ccggtgttcc tccgaccggg ccaggtggtc cggaccgtga tcgacgggat
      601 cggcgagctc gtcaacgagt gcgtcgcgga gaccgaccct cgtacgaccc cgtagccgcc
      661 gaaggggggt cagccgacgc ggtaccggtg gaccgcgagc gcggtgaaga ccgagacgat
      721 cacgacccac cagatcgcga aatggcccgc ggcggtcgtg agatgggtgt ggctcactgc
      781 gaacagcgtc ttgtcgccaa gcgcgagacc acgcgcggtg tcgatcgccg aggtgatcgg
      841 gttccagcgc gccaccagct gcatccaccc cggaagacga gacactggcg cgaacgctga
      901 gctggtgaac accaggggca gcaccgggat gaacaccgcc ggctgcaccg tctcggggtc
      961 cttgaccgcc aatccaatcc aggccgagaa cgcggtgagc gccatcccga acaagacgac
     1021 gaccacgacc atccccacgg ccgcgaggac accgttgtgg aacgagaacc cgatcgcgag
     1081 cgaggcaccg acgagcagca ccgactggat cccgaggcgc aggctgtcac tcgtggtgcg
     1141 cccgacgagg aacgccgacc gggcgatcgg caacgaccgg aaccggtcga ccatgcccga
     1201 ttggaggtcg tacgcgatcc caacgccgga ccccatcgcc gagaaggtga tcgcctggac
     1261 gagcacggcc gggacgacga agtccttgta gctggtgcca cgcccgaccg tggcaatcgc
     1321 gccgaacacg aacgcgaaca gaaccagctg catcaacggc tggatggtcg agaacacgat
     1381 gaggcccggg ctgcgcagca ccttgcgcag gttgcgtccc gcgatcaacc cgatgtcggc
     1441 cacgacccgc cccggcggga gcgcggcccg ggcttcgacc gtcatcggag gggcgtcggc
     1501 gagattccgc atcgtcaggc ccgcacccgc cgtcgcgccc gcggctcggg agcgtcggga
     1561 cggggctctt ccgcatgatg accggtcagg ctgagaaaga cgtcgtcgag cgacggtcgc
     1621 cgcaacccga ggtcggcgac tggcaccccg gcggcatcca gagcccggac cgcgtcgacg
     1681 agcgcggccg cgcccgactg gacggggacg agaacggtgc ccagctcggc gtcgatcgtg
     1741 atctcggcct tcgggacgct gaacgccgtc gccagcgtcg ccgcggcgtc gccgacccgg
     1801 gcccggtcac cgacccgaag atcgaggacg tcaccgccga ccttggtctt gagctgatcg
     1861 gcagtgcctt cagcaatgat gcgacccccg tcgatcacgc tgatgttctg ggcgagctga
     1921 tcggcctctt ccatgtactg cgtggtgagc acgatcgtgg tcccctcgtc gcgcagatcg
     1981 gtgatgacgt cccagagccg cagccggctg cgagggtcga gcccggtggt cggctcgtcg
     2041 agaaacagca ccgccggcgt cgcgatcagg ctcgccccga ggtcgaggcg gcgtcgcatc
     2101 ccaccggagt agccacgcaa cacccgatcc gcggcatccg cgaggtcgaa cgcgtccagc
     2161 agcgcctcgg cgcgctgttg ggccaccacc ttgctcaggc ggccgagccg gccgacgagg
     2221 acgaggttct cgcgtcccgt gagatcctcg tcgaccgcgg cgaactggcc cgtcagcccg
     2281 atcaccgccc ggacgtgctg gggttcgcgg acgatgtcgt agcccgccac ccgggctcgc
     2341 ccgccgtcgg gtcgcaacaa ggtcgacagg atccggatca ccgtcgtctt gccggcgccg
     2401 ttcggtccga ggagccccac cacggttccc tcggggacga cgaagtcgac accgccgagc
     2461 gcgacgaacg agccgaaccg tttgatcagc ccttcgacca cgacacccgt cgaatccacg
     2521 acgagcgaac ctatcggctg tcgggtgcga tctcgatcac gcgccgctcg gctcgtcccg
     2581 gcggacggct tgccccgaat ttcgttttgc acctaacctt tcgtcgccta tggaagagcg
     2641 ctcggccgtc agctgtccat cacgggacag atggtgcggg accgcttcga cggctgcctg
     2701 aacgagcacg gggcgagcct gaccacctgg gcggtgctgc ggagcaccga gcacgaggac
     2761 ggcctgagcc aacgagagct ggcgtcgcgg atgagcatcg agagcccaac cctcgttcgt
     2821 caccttgacc ggctcgaaga cgaggggctc gtggttcggc gccgcgatgc ccaggatcgt
     2881 cgagtcgtcc gggtcgggct gacaccggcc ggccatcgtc gctacgccga gctgcgcgac
     2941 gtcgcggcga acgtcgactc ccagctccgc tccctgttgg acgacgatga gatcgcgacc
     3001 ctcgaacgcg cgcccccgat cccctccccg gaccccggag atcagctacg gcgcgacctc
     3061 ggaggcgagc cgctcgtggc acgggcccga ccgagcggcc atcggggctc aagaccgcag
     3121 gctgggggct cacccggggt cgggccgggg gctccgggcc ggcctgagca ccggggcgcg
     3181 agagatcggt gaaccgcttg gtatagatgc ggatggtgtt ggcctgatgg gagtcctcga
     3241 ggacgtcgga cgcagcctgc gtgagggctt cttcatgttc tgggagaccc tctggcccct
     3301 gatcctcggt ttcgcgctgt ccggcgcagt gcaggcgttc gtctcacggg accagatgga
     3361 acgagtcatg ggcgaccatc gaccgcggac ggtgacacgg gccagcgcca tcgggatggc
     3421 atcgtcgtcg tgctcgtacg cggcgacggc gatggccaag tcgttgttcg agaagggcgc
     3481 cgacttcacg acggcgttga tcttcatgtt cgcgtcgacg aacttggtgc ttgagctcgg
     3541 gatcgtgctc gtggtcctcc tggggtggca gttcgcgctg gcggagttcg tcggcggccc
     3601 gatcatgatc gcgctgctcg cactcaccgg ctcgttcgtg gtcggcaagt cgatcgccgc
     3661 ccgggcgcgc cgcaacctgc gtgcggcacg agaagcggac gatcccgacg cgatggccgg
     3721 cgtcagcgag gagcgccagg cggagctgga acgcgaaccg tggcgcaaga agctgcgctc
     3781 ccgggcggcc tggtccgacg cggcgaccta caccgtcgcc gacctgacca tgctgcgacg
     3841 cgagctggcc gtcggctacc tcgtcgccgg gttcctcacc gtcctcgtcc cggtccaagt
     3901 gtggaacgac gtgttcatcc acgggcacgg atggtggaca tcggtcgaga acgtcgtggt
     3961 cgggccgttc gtcgcgatca tcagcttcgt ctgctcgatc gggaacgtgc ccctcgccgc
     4021 cgccctgtgg cacggcggga tcagcttcgg tggcgtcatc gcgttcatct tcgccgacct
     4081 catcaccttt cccttgctgc tcatctaccg ccgctattac ggctggcgag tgacgctgcg
     4141 gctgctgctg tggttctggg ccgtcatggc cgtggccggc ctcgcggtcg agggtctctt
     4201 cgacgtcacc ggcctcatcc cccgcacccg accccgcgac atcgttccca ccaccttcca
     4261 gtggaactac acgacgttct tgaacctcgc gttcctcgtc gtcgccggct acctgtactg
     4321 gctgtaccgg aaccgggacc gcttcggtgg cggcgccggg tacgcgatcg acccggtgtg
     4381 cggcatgcag gtccagaccg ccaacgcgcc ggcgtcgacc caccacgacg ggcggtccta
     4441 ctggttctgc tccgaccggt gcgcggacca gttccgccgc gaccccgacg tgcacacccc
     4501 ggccgccggg ccggtgtcga acccggctcc gggcccgagc tgacaggccg cggcgcgcga
     4561 ccgggtcccg cggctcggct cagcgggtga cggggacgct gatcgcgacc gtcgcgcccc
     4621 cgcccggcgt gtcgtcgagg ctcagctccc cgtccatggc ctcgacgaac ccgcgggcca
     4681 cggccaaccc gagaccgacg cccgtgccct ggggattgtc acccaggcgc tggaacggct
     4741 gaaacacctc ctcgcgcttc gagcgcggga tccccggccc gctgtccacg atggtcagct
     4801 cgacccgctc ggcgctcgcg ccggcgaaga tccgcaccgg ccggtccggc ggtgaggcct
     4861 gcagcgcgtt cgcgacgacg ttggcgatcg cccgttccaa cagggcggca tcggctcgaa
     4921 cccggggcag ggtctccggg acgtcgacta ccacccggcc cgtggtgtca cccaggctct
     4981 gcaacgccgc ggcgaccacc tcttcgagac cgacgtcgcg ccacaccagc tcgaaggatc
     5041 cggtctggag gcgactcatg tcgaggaggt tgccgaccag cttggtgagc cggtcggtct
     5101 cctcgtcgat cgcgtcgagc agctcgttct tggagcccgc gtcgagggtc acgtccggtt
     5161 ggagcaggct cgtcaccgcg gccttgatcg ccgccagcgg ggtgcgcagg tcgtgggaca
     5221 ccgcggcgag gagcgcggtg cgcagctcgt tcgcctcggc gagcccttcc gccgcggcgg
     5281 cctcggcccg caggcggcgt cgctccaccg ccaacgcgag ctgcccgacg aaggcccgca
     5341 gcatctgcag gtcttcctca ccgatgccct cgccgcacac cgcgagcgtc gtgtcggcgt
     5401 cgagggagac gctcacctgg gcgtcgtcgg gagtctgcgg gaccggatcg ccggcgctga
     5461 cctcgacccg ccagccgtca ccggagcggc gcagcaccga caccgactgg cacccgaagg
     5521 cgtctcgcag cccggccatc aacgctggca ccgggtcgtg gtcgctgagc agggtgccgc
     5581 tcagcgcggc gagggtctcg gcgtccgctt gcgcccggga cgcctgcgct cgtcgtctcg
     5641 ccacccggct gaccagctcg ccgaccacga ccgcgaccac gacaaacacg atcaaggcga
     5701 cgaggttgtc gtcgcggtcc acgttcaggg tgtggaacgg ctgggtgaag aaccatttgg
     5761 agagcaagaa cccggccacg gcgctcagca ccgcggggac gagccccccg acggcggcga
     5821 ccccgatgac gacgagcagg tagaagagca ggtcgctggg gaggctgacg tcgctccgca
     5881 ccgcggtgag tgccaccgtg agcagcggca gggccaggac cgcgagcacg caggcgatgc
     5941 cttgacggcg acgcgagatg ccggagcgga cgacccggat cggtgagccg acctcgcggt
     6001 ccgggtcggg ggtctcggtg gcgatcacat gcacgtcgat ggtgtcggcc caccgcagga
     6061 tccgcgagat caccgacccg tggaccagct ccgtccaccg ccgccgccgg ctggccccca
     6121 ccacgagctg ggtggcccgt tcggaccgcg cgaactgcac cagcgcctcc ccgacgtcgt
     6181 cgccgccgac ctcgtggtac gcgccgccga ggtccgcgag gaggtcgcgg tggctcacga
     6241 gcaagtccga tgtccgcgag gcgaggccgt ctgtcgggcg gacgtggacg ccgatcaagt
     6301 cacctttggt ccggcgagcc atccgcgccg cccgtcggat cagcgtgtca gcgccgggcg
     6361 cgccggtcag ggccaccacg acccgctccc gggtctccca ggtgctctcg atcccgtgcg
     6421 cctcgaggtc c
//
LOCUS       DATCCH010000448         2149 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_620993, whole genome shotgun sequence.
ACCESSION   DATCCH010000448 DATCCH010000000
VERSION     DATCCH010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620993"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="VIJ44_08660"
     CDS             <1..530
                     /locus_tag="VIJ44_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009156988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEY5166008.1"
                     /translation="PACRNGDLEMPHEPEVEQRAAQPYVGIRMPVTMEGFGAVIDAGF
                     PEIFGWLEEHGIARAGPPFIRYLVIDMEAQLEVELAVPVRGEVEGDGRVRVDVLPAGR
                     YVTFRHVGSYDGLIASNAALQQWAQEQGIALDSWATERGSAWRGRVEHYVTDPSTEPD
                     PAKWEVDVAYLATGS"
     gene            complement(788..1861)
                     /locus_tag="VIJ44_08665"
     CDS             complement(788..1861)
                     /locus_tag="VIJ44_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006537992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5166009.1"
                     /translation="MEPIKIGWLGAALDGPGGGYDKIHRLAFDEATEQGILSRPYEFV
                     LHSENGLPNGSAKSAIDGFKYLVDEGCIAVAGAYSSDNGMVVGPYANELGVPLVSWCG
                     TERFWGPYCFRLGNGDCGGDPALIVGWLKKHGYERVAVLSEVSPNGEEYFRYFRMECR
                     RRGVTVAAVETVSQTPADLAHNLDNLRSVGADALVYLGYGILAASGLLREALDKLAWD
                     PPRIMTTAFMFYLMGFEKFEGWVGIDQLCPDNRLQQRFHERYVARYGDDPPMWPNAIP
                     VLAFDTARVLVEAAFRAPVLTGWGMKEGFEQIRFMPSATGGPQTHIAGSPYDHQLFKG
                     DWLLYGRIRNGKLEFEGLFEPWE"
BASE COUNT          355 a          690 c          731 g          373 t
ORIGIN      
        1 tcccggcttg ccggaacgga gatctcgaga tgccgcatga acccgaggtc gagcagcgtg
       61 ccgcgcagcc gtacgtcggt atccggatgc cggtcaccat ggaggggttc ggagcggtca
      121 tcgacgcggg tttcccagag atcttcgggt ggctggagga gcacggcatt gcgcgtgccg
      181 ggccgccgtt cattcgctac ctcgtcatcg acatggaggc ccagctcgag gtcgagctgg
      241 cggtgccggt acgaggtgag gtggagggag acggccgcgt ccgcgttgac gtcctcccgg
      301 ccggccggta cgtgacgttc cggcatgtcg ggtcgtacga cggtctcatc gccagcaacg
      361 cggcgctgca acagtgggcc caggagcagg gcatcgcgtt ggacagctgg gccaccgaac
      421 gcggttcggc ttggcgcggc cgcgtcgagc actacgtgac ggatccgtcg acggagcccg
      481 acccggccaa atgggaggtc gatgtggcgt acctggcgac cggaagttga gcggtgctcg
      541 tcgtgacgac ggggcgtcgg cgatcgcgtt ctattgggac ggttttgaag cggtggcaca
      601 tcggcgcggg cggtggcgtc gaggccggct tatcggtgga tggtgtcgac cggcttcctc
      661 gctcaggaga cactgagccg tagaacgtgc cccgggcgcc ggcggtcaca tgaccgtgcg
      721 aaacgagtta ctgacgaccg gcgcgggatc ccggagaggc atggattgaa ctcgacctcg
      781 gccgtcatca ctcccacggt tcgaagagtc cttcgaactc gagctttccg ttgcggatgc
      841 gtccgtagag gagccagtct cccttgaaga gctgatggtc gtacgggctg ccggcgatgt
      901 gggtttgtgg cccgccggtg gcgctgggca tgaatcggat ctgctcgaag ccttccttca
      961 tcccccagcc ggtgagcacc ggtgctcgga acgctgcttc gaccaacacg cgcgcggtgt
     1021 cgaaggcgag gacggggatc gcattcggcc acatcggcgg atcatcgccg taacgcgcga
     1081 cgtagcgctc gtggaaccgt tgctggaggc gattgtcggg acagagctgg tcgatgccga
     1141 cccagccctc gaacttctcg aagcccatca ggtagaacat gaatgccgtg gtcatgatgc
     1201 gcgggggatc ccacgccagc ttgtcgaggg cttcacgaag caagcctgag gcggcgagga
     1261 tgccgtagcc gaggtagacg agggcatcag caccgacgct gcgaaggttg tcgaggttgt
     1321 gcgcgaggtc ggcgggggtt tggctcacgg tctcgaccgc ggcgaccgtg acgccgcgac
     1381 gacgacattc catgcggaag tagcggaagt actcctcacc gttgggtgag acctcgctga
     1441 gcaccgcgac ccgctcgtag ccatgcttct tgagccaccc gacgatgagg gcgggatcgc
     1501 ccccgcagtc accgttgccg agccggaagc agtaggggcc ccagaaccgc tcggtcccgc
     1561 accacgacac cagtggcacc cccagctcgt tggcgtaggg gcccaccacc atcccgttat
     1621 ccgagctgta cgcgccggcg acggcgatgc agccttcgtc gacgaggtac ttgaacccgt
     1681 cgatcgcgct cttcgccgac ccattgggca agccgttctc ggagtgcagc acgaattcat
     1741 acgggcggct caggattccc tgctctgtcg cctcgtcgaa ggccaggcgg tgaatcttgt
     1801 cgtacccgcc acccgggcca tccaaggcgg caccgagcca cccgatcttg atcggctcca
     1861 tcccatttcc tctcgttcgg gcttcagtcc gacgacttct tcgaacatcc ttatctagcg
     1921 aaggaacgtg ggggccgcca agcttcgcgc tgctcgcacg accttggtcc tcaccgggtg
     1981 gccgcacgtc gcccgcggca ccgcctcccg acgagcagac atccagacgt tgggttctga
     2041 gtgctcgaga ccttccccgc gtcgcgggcg cggcgattca cccgatcggc acctcgaccg
     2101 cctcgccgcc ggagatccac gaacggcccg cggctttgac gtcggccca
//
LOCUS       DATCCH010000449         3002 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_363654, whole genome shotgun sequence.
ACCESSION   DATCCH010000449 DATCCH010000000
VERSION     DATCCH010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363654"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..2067)
                     /locus_tag="VIJ44_08670"
     CDS             complement(124..2067)
                     /locus_tag="VIJ44_08670"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEY5166010.1"
                     /translation="MGRPSGQIGVTDDQGRARRHIPALDGLRGAAVAAVLLFHAGHLT
                     GGWLGVDLFFVLSGFLITSLLLAERARRDRISLSAFWARRGRRLLPALFLVLIGVGVY
                     AVVWAAPRELAQIRSDGLATLGYVANWHQIGQGHSYWDLFRAPSPLEHTWSLAIEEQF
                     YLVWPIVVVGLLAWGRKPRRVLIAALIGIVASFTTMLVLYSPGSNPQRVYLGTDTRAS
                     SILIGAALAALVATHGWTARRESQVVLEGLGVVSLGFLGWAWLRLSGTTPAVYQGLLL
                     ACSLAAGLLIAAAAHPRPGLVARVFSFRPLRALGVISYGVYLWHWPIYLVADSSRVGL
                     SGWPLTGVQVAITLAFAITSYYLIESPIRHGALRGWRIRALAPAALAATAVIVVVATI
                     PPPTPRTNPVAALRHLTAAAQSQSKTVTTPTTTSSLPPRPFKLTVTGDSIAFRLMPAF
                     QSLGGPLGFSTTDRSTVGCALERGATAHRLPDGTITPADLNCSTGWATAVAQDRPDVV
                     FVSLAGQVVGDWQINGQWVHPCEPAYDQWYEGQLVEGLSVLTSGGARVALALPAPAFG
                     GDFAARGECIRAVEQRAANEVPGAHTVDFKTLVCPQNRCISEIDGITLREDGMHYIGP
                     AAELVVRWLVPRLRVVAAAPSLP"
     gene            2245..2571
                     /locus_tag="VIJ44_08675"
     CDS             2245..2571
                     /locus_tag="VIJ44_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5166011.1"
                     /translation="MLWFERQIVATLMSPAVDERWRDEIEAYVDGALRSMPEYLRAGV
                     GAESLAFGAWARIQGALGRLDRASLDQRVERWKTSRFDPVRQYVRLLQSLVLFAENEL
                     IPETTP"
     gene            2568..>3002
                     /locus_tag="VIJ44_08680"
     CDS             2568..>3002
                     /locus_tag="VIJ44_08680"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEY5166012.1"
                     /translation="MTTLETDVLIIGSGAGGAVTAATLAGAGRDVTVVEEGPWIDPDA
                     MEPFSLDEMVTKYRHRGLAAALGTPGIAYAEGRCVGGSTEINSGLYHRLPAELAEEWR
                     RSYQIDEFTPAVLDRYAAGIESELSVSKLPGAPPPSSAVLERG"
BASE COUNT          499 a         1052 c         1050 g          401 t
ORIGIN      
        1 ggctgctgcc gcgggtgctc agcctcgttg ccccggcggc caactcgacc agcgcgtcgc
       61 agagctcggc caagaagtag taacgggccg cgcggcccca cctggcgacc gcgacatgcg
      121 ccgctacggc agcgagggcg cggcggcgac gacgcggagg cggggcacga gccaccgcac
      181 cacgagctcg gccgcgggcc cgatgtagtg catcccatcc tctcggagcg tgatgccgtc
      241 gatctcgctg atgcaacggt tctgggggca cacgagggtc ttgaaatcaa cggtatgcgc
      301 gcctggcacc tcgttcgcgg cgcgctgctc gaccgctcgg atgcattcgc cgcgggcggc
      361 gaagtcaccc ccgaacgcgg gtgccggcaa ggcgagcgcg acccgggctc ctccgctcgt
      421 gagcaccgag agtccctcga ccagctggcc ttcgtaccac tggtcgtagg cgggctcgca
      481 cggatgcacc cactggccgt tgatctgcca gtcgccgacg acctggccgg ccagggagac
      541 gaacacgacg tcgggtcggt cctgggccac tgcagtggcc caaccggtgc tgcagttgag
      601 gtcggcgggc gtgatcgtcc cgtcgggaag gcggtgtgcc gtcgcgccac gctcgagggc
      661 gcacccgaca gtggagcgat cggtggtgct gaacccgagt ggtccgccca gactctggaa
      721 cgcgggcatt agccggaacg cgatcgaatc gccggtaacc gtcagcttga acggccgcgg
      781 tggcaggctc gacgtcgtgg tcggcgtggt gacggtcttc gattgggact gtgcggccgc
      841 ggtgagatgt cgcaacgccg ccaccgggtt ggtgcgcggt gtgggcggtg ggatggtggc
      901 gacgacgacg atcactgcgg tcgcggccaa cgccgccggc gcgagcgcgc ggatacgcca
      961 cccccgcagc gcgccgtggc ggatcggcga ttcgatgagg tagtagctgg tgatagcgaa
     1021 cgcgagggtg atcgcgacct gcacgccggt gagcggccag ccgctcagac cgacccgcga
     1081 cgagtcggcg acgaggtaga tcggccaatg ccacaggtag acgccgtagg agatcacgcc
     1141 gagtgcgcgg aggggacgga acgagaagac ccgagccacc agcccggggc gcgggtgcgc
     1201 ggcggcggcg atgagcagcc cggccgccag ggagcacgcc aggagcaatc cctgatacac
     1261 cgcgggggtc gttcccgaga gtcgaagcca cgcccacccg aggaagccga ggctcacgac
     1321 tccgagccct tctagcacca cctgggattc acggcgggcg gtccagccgt gcgtggccac
     1381 cagcgccgcc agcgccgcac cgatcaggat cgacgacgcc cgggtgtccg tgccgaggta
     1441 cacccgctgg ggattcgatc ccgggctgta cagcacgagc atcgtcgtga acgacgccac
     1501 gatgccgatc agcgccgcga tcagcacccg gcgcggtttc cgaccccacg ctaagagtcc
     1561 gacgacaacg atgggccaga ccagatagaa ctgctcctcg atcgccaggc tccacgtgtg
     1621 ctccagcggt gacggggcgc ggaacaaatc ccagtacgag tggccctgcc cgatctgatg
     1681 ccagttggcg acgtagccga gcgtcgcgag gccgtcactg cggatctggg ccagttctcg
     1741 cggcgcggcc cacaccacgg cgtacacgcc cacgccgatc aagaccagga acagcgccgg
     1801 gagcagccgt cggcctcgac gcgcccagaa cgccgagagc gagatccgat cgcgccgcgc
     1861 ccgctcggcg aggagcagcg acgtgatgag gaagccggac aggacgaaga agaggtcgac
     1921 cccgagccaa ccaccggtca agtgcccggc gtggaaaagg agcaccgcgg cgacggcggc
     1981 gccccgcaga ccgtcgagcg cggggatgtg gcggcgcgcc cggccctggt catcggtgac
     2041 gccgatctga cccgacggtc gacccactgc ctcgatgtcc ccctcccccc gctcgtggcc
     2101 gcaggtatcc gccgcgggat tgtgacctgc gctcccggag gctcacggcg cggaccgcga
     2161 caccagtttg gcccgtgtcg tgggtcttgg gggcgtcaac gccaccgagg gggaaacgaa
     2221 ggccccccgg taggctttcg ccccatgttg tggttcgagc gccagatcgt cgcgacgctc
     2281 atgagcccgg ctgtcgacga gcgatggcga gacgagatcg aggcctacgt cgacggcgcg
     2341 ctgcgatcca tgcccgaata ccttcgagcc ggggtgggcg ccgaatcgct cgccttcggg
     2401 gcgtgggccc gaatccaggg agcccttggt cgcctcgacc gcgcgtcgct cgaccagcga
     2461 gtcgagcgtt ggaagaccag ccgcttcgat cccgtccgcc agtacgtgcg gctgctccag
     2521 tccctggtcc tcttcgcgga gaacgagctg atccccgaga cgacaccgtg accaccctcg
     2581 aaaccgacgt gctcatcatc ggctccggcg ccggtggtgc agtcactgcc gcgaccctgg
     2641 ccggagccgg ccgagacgtg accgtcgtcg aagagggtcc ctggatcgac cccgacgcca
     2701 tggagccctt ctccctcgac gagatggtca ccaagtaccg ccaccgaggc ctggccgccg
     2761 cccttggcac ccccggcatc gcctacgccg aggggcgctg cgtcggtggc agcacagaaa
     2821 tcaacagcgg cctctatcac cgactccccg ccgagctcgc cgaggaatgg cgccgctcgt
     2881 atcagatcga cgagttcact cccgcggtcc tcgaccggta cgcggccggc atcgaatccg
     2941 agctgtcggt ctccaagctc cccggcgccc cgccgccgtc ctccgcggtg ctggagcgag
     3001 ga
//
LOCUS       DATCCH010000450         5340 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_155354, whole genome shotgun sequence.
ACCESSION   DATCCH010000450 DATCCH010000000
VERSION     DATCCH010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5340
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155354"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="VIJ44_08685"
     CDS             complement(<1..614)
                     /locus_tag="VIJ44_08685"
                     /inference="COORDINATES: protein motif:HMM:NF025389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4239 domain-containing protein"
                     /protein_id="HEY5166013.1"
                     /translation="MTWLGSLPAWAIFPLFAAGTLLLTVAVDGILRRRLVPDDARANA
                     SPSAATTLQALATIYAVLAAFVIVDEYGQLRDAQSQVATKAAELSVIVENSRNLPLDS
                     GARIRAATIDYARVEVRDGLPKLVQTSQPSIQSDAALERIYRVVASIEPSAESDKIAY
                     QQILVALAGVSETRTNLISSAKATIPVALFFILAVLGVAILGVGT"
     gene            complement(630..2846)
                     /locus_tag="VIJ44_08690"
     CDS             complement(630..2846)
                     /locus_tag="VIJ44_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEY5166014.1"
                     /translation="MRRRFPFKPSQVVLVLGLLIAAGALASGVLPRITEWHDNSHTAR
                     QVFVDVPDPIYWMFYATVATMLFVCAWLVSMRVRNYARGGPDDRRTTRANAHRRLREF
                     RSGVWMRTLLRDPAAGIMHSCIYFGFLVLFAATVILEIDHQLPPSLKFLHGTVYEAYK
                     ATANLFGIVFLVGILWAIGRRYVQRPYRIRIKTKPEDAVILATFLIIGVTGFLIEGFR
                     IALENRPHFEQWSFVGWGLSALWSNWAHGTLSDLHRTMWVIHFGAFLAFLVILPTTKL
                     RHMFTSPMNMYLGPKDRPKGAMKPMPNLMETELETFGASTVEDFTWKQLFDTDACTIC
                     GRCTSVCPAHATGKPLDPREIVLKVGEVMAATGDPPVSPPVGLVDDITVTADSMFERI
                     TAEELWACTTCKACDEICPVDIEILDKILDMRRYLSLMESDFPTELGATYRSMENSSN
                     VYGMNQGERADWAKDLDGIPIIDSSGPFDHEYLYWVGCAGSFDDRNKRASRAIAKLLH
                     RAGIDFAILGPSELCTGDPARRSGNEYIFQMLATQNIEALQGLGVKKIITQCPHCFNT
                     LKNEYPQLGGNYEVVHHSQFLMELIDDGRLALQGATLEERVTYHDSCYLGRHNDVYLA
                     PRNVVGSLGGIDIVEMPRNGTRGMCCGAGGARMWMEEHTGKKVNTERSQEALATGATR
                     IAVACPFCYVMMEDGVKEAQRDDDVKVQDIAEVLLEAIERIETAPVPPTAEFTSGI"
     gene            complement(2914..3336)
                     /locus_tag="VIJ44_08695"
     CDS             complement(2914..3336)
                     /locus_tag="VIJ44_08695"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY5166015.1"
                     /translation="MTRRSALPDLAKVPLFSACSKKDLQQINRRAESVQVQAGKVLVR
                     EGAAGAEFFVILDGTAEVSMHGKNVATLGPGAFFGDLALLDKAPRNATVTAVTPMELM
                     VLGQREFAAMIDEVPGFAHKLLSGLAHRLREYDVKTWE"
     gene            3445..5307
                     /gene="dnaK"
                     /locus_tag="VIJ44_08700"
     CDS             3445..5307
                     /gene="dnaK"
                     /locus_tag="VIJ44_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEY5166016.1"
                     /translation="MSKAVGIDLGTTNSVVSTLEAGEPAVIPNAEGSRTTPSVVAFSK
                     TGEVLVGEVAKRQAITNPDRTIRSVKRHMGTSWSIDIDGQHYTPQEISARILQKLKRD
                     AEAYLGDTVQQAVVTVPAYFDDAQRQATKEAGEIAGLEVLRIINEPTAAALAYGLDKE
                     NDLTILVFDLGGGTFDVSVLELGDGVFEVKSTAGNTHLGGDDWDQRVIDWMVAEFKGA
                     HGVDLAADKMATQRLKEAAEKAKIELSSLQETTINLPFITATQEGPLHLELSLSRAKF
                     QELTADLLDACKGPFEQAIRDSGLTSDGIHRVILVGGSTRMPAVQGVVKDVTGKEGDK
                     GVNPDEAVAVGAAIQAGVLKGDVKDVLLLDVTPLSLGIETKGQIMTRLIERNTTIPTK
                     RSEVFTTADDNQPSVEIKVLQGESDMAYRNKMLGTFQLVGMPPAPRGVPQIEVTFDID
                     ANGIVHVSAKDLGTGKEQAMTITGGTALGKEEIDRMVKEAEAHAEEDRKRHDEAETRN
                     NADSLVFQTEKLLKEQADKVSAEDRTKIESALGELKTAIAGEDIDAVRQKHEALLTAS
                     QEFAQRLYQAAQAQQSASGTSGEGAASDTAQPSDDEVADAEIVDEGHDGHEKSA"
BASE COUNT         1001 a         1696 c         1796 g          847 t
ORIGIN      
        1 gtgccgacgc cgaggatcgc gacgcccaac accgcgagga tgaagaacag cgccaccggg
       61 atcgtcgcct tggccgagct gatcaggttc gtgcgggtct cggacacccc ggccaacgcc
      121 accaggatct gctgatacgc gatcttgtcc gattcagcac tgggttcgat cgaggccacc
      181 acccggtaga tgcgctcaag ggcggcgtcg gactggatgc tgggctgcga ggtctgcaca
      241 agtttgggaa gaccgtcccg gacctcgacg cgggcgtagt cgatcgtcgc ggcccggatc
      301 cgggcgccgc tgtcgagagg gaggttgcga ctgttctcga cgatgacgct cagctcggcg
      361 gccttggtgg cgacctggga ctgggcgtct cggagctggc cgtactcgtc gacgatgacg
      421 aacgccgcca acaccgcgta gatggtcgcc agggcctgca gcgtcgtcgc ggcgctcggg
      481 ctcgcgttgg cacgagcatc gtcggggacc aaccggcgcc gcaagatgcc gtcgacggcc
      541 accgtgagca gcaaggtccc ggcggcgaag agcgggaaga tcgcccacgc ggggagcgag
      601 ccgagccagg tcatccgggg cggatcagct cagattccgg aagtgaactc ggcggtcggt
      661 gggaccgggg cggtctcgat ccgctcgatc gcctcgagga gcacctcggc gatgtcttga
      721 accttgacgt cgtcgtctcg ctgcgcctcc ttcacaccgt cttccatcat cacgtaacag
      781 aacgggcagg ccaccgcgat ccgagtcgcg ccggtggcga gcgcttcttg ggaacgctcg
      841 gtgttgacct tcttgccggt gtgttcctcc atccacatgc gggctccgcc ggcgccgcag
      901 cacatgccgc gcgtcccgtt gcggggcatc tccacgatgt cgatgccacc taacgacccg
      961 acgacgttgc ggggagcgag gtacacgtcg ttgtggcgac cgaggtagca cgagtcgtgg
     1021 taggtcacgc gctcttcgag cgtcgcgcct tggagggcga gtcgtccgtc gtcgatgagc
     1081 tccatgagga attgcgagtg gtgcacgacc tcgtagttcc cgccgagctg tgggtattcg
     1141 ttcttgaggg tgttgaagca atgcgggcat tgcgtgatga tctttttcac gccgaggccc
     1201 tggagggcct cgatgttctg ggtggcgagc atctggaaga tgtactcgtt gccggaccgt
     1261 cgagctggat cgccggtgca gagctcgctc ggtccgagga ttgcgaagtc gatcccagcg
     1321 cgatgaagga gtttggcgat tgcccggctg gcgcgcttgt tgcgatcgtc gaagctgccg
     1381 gcgcagccga cccagtacag gtactcgtgg tcgaagggcc cggagctgtc gatgatcggg
     1441 atcccgtcaa gatccttcgc ccaatcggct cgttcgccct ggttcatgcc gtacacgttc
     1501 gacgagttct ccatcgaccg gtaggtggcg ccgagctcgg tggggaagtc gctctccatc
     1561 agggacaggt accggcgcat gtcgaggatt ttgtccagga tctcgatatc aaccgggcag
     1621 atctcgtcgc aggccttgca ggtggtgcag gcccagagct cttcggcggt gatgcgctcg
     1681 aacatggaat ctgcggtcac ggtgatgtcg tcgacgagcc cgaccggtgg cgagaccggg
     1741 ggatctccgg tcgcggccat gacttcaccg actttgagga cgatctcacg cggatcgaga
     1801 ggcttgccgg tggcgtgcgc cgggcagacc gacgtgcagc ggccgcagat cgtgcacgcg
     1861 tcggtgtcga agagctgctt ccaggtgaag tcctcgaccg tggacgcacc gaaggtctcg
     1921 agctcggtct ccatcaggtt cggcatcggc ttcatcgcgc ccttgggccg gtctttcggt
     1981 ccgaggtaca tgttcatcgg tgacgtgaac atgtgacgca gcttcgtggt cgggaggatg
     2041 acgaggaacg cgaggaacgc accgaagtgg atcacccaca tggtgcggtg caggtcggac
     2101 agggtcccgt gcgcccagtt ggaccacagc gccgacagtc cccaaccgac gaatgaccac
     2161 tgctcgaagt ggggccggtt ctcgagcgcg atccggaacc cctcgatcag gaaccccgtg
     2221 acgccgatga tgaggaacgt cgcgaggatc accgcgtcct cgggcttggt cttgatccgg
     2281 atccggtagg gccgttgcac gtatcggcgt ccgatcgccc acaggatccc gacgaggaag
     2341 acgatcccga acaggttcgc ggtggccttg taggcctcgt agacggtccc gtggaggaac
     2401 ttcagcgacg gtgggagctg atggtcgatc tcgaggatca ccgtcgcggc gaacaacacc
     2461 aggaagccga agtagatgca ggagtgcatg atccccgcgg cagggtctcg gagaagggtc
     2521 cgcatccaca cgcccgagcg gaactctcgc agccggcggt gcgcgttggc gcgagtcgtc
     2581 cgccggtcgt cgggaccgcc gcgcgcgtag ttgcggaccc gcatcgagac cagccaggcg
     2641 cagacgaaca gcatcgtcgc gaccgtggcg tagaacatcc agtagatcgg gtccgggacg
     2701 tcgacgaaga cctgccgggc cgtgtgtgag ttgtcgtgcc actcggtgat ccggggcaac
     2761 acaccggacg ccaacgcacc ggcggcgatg aggagcccga ggaccaagac gacctggctc
     2821 ggcttgaacg ggaaccgacg gcgcacggaa ggaaacctaa tactgcgcct cttggtgggc
     2881 cgactaccgg tcccgacgct cagggaaggt cgatcactcc caggttttga cgtcgtattc
     2941 gcgcagtcgg tgcgcgagcc cgctcaggag cttatgggcg aagcccggca cctcgtcgat
     3001 catcgccgcg aactcccgct ggccgagcac catcagctcc atcggcgtca cggcggtcac
     3061 cgtcgcgttg cgaggtgcct tgtcgagcag ggccaggtca ccgaagaacg ccccgggtcc
     3121 gagcgtggcc acgttcttgc cgtgcatgga cacctcggcg gtcccgtcga ggatgacgaa
     3181 gaactcggcc ccggcggctc cctcccggac gaggaccttc ccggcctgga cttggacgct
     3241 ctcggcccgc cggttgatct gctggaggtc ctttttcgag caggccgaga acaggggaac
     3301 cttcgcgagg tcgggcagcg cacttcgccg agtcatgtcg gcaatgtacc gagggtgtca
     3361 acacgaccca tccgggaata gggcccggga ccagaaagtt gatactgtca cactcaagat
     3421 tttccacatg gaggcaaata gatcatgtcc aaggccgtcg gtatcgacct cggcaccacg
     3481 aactcggtcg tctcaactct ggaggcgggc gagccagccg tcatccccaa cgccgaaggg
     3541 tcgcgaacga ccccctcggt ggtcgcgttc tccaagaccg gcgaggtgct cgtcggggag
     3601 gtcgccaagc gccaggcgat cacgaacccg gaccgaacca tccggtcggt caagcgccat
     3661 atgggcacgt cgtggagcat cgacatcgac gggcagcact acacgcccca ggagatctcg
     3721 gcccgaatcc tccaaaagct caagcgtgac gccgaggcgt acctgggtga caccgtccaa
     3781 caggccgtgg tcaccgtgcc cgcgtacttc gacgacgccc agcgtcaggc caccaaggag
     3841 gccggcgaga tcgcgggtct cgaagtcctg cgcatcatca acgagccgac cgcagccgcg
     3901 ctcgcgtacg ggctcgacaa ggagaacgac ctcaccatct tggtcttcga cctcggcggg
     3961 ggaacgttcg acgtgtcggt cctcgagctc ggcgacgggg tcttcgaggt caagtcgacc
     4021 gccgggaaca cccacctcgg tggcgatgac tgggaccagc gggtcatcga ctggatggtc
     4081 gccgagttca aaggcgccca cggcgtcgat ctcgcggccg acaagatggc gacgcagcga
     4141 ctcaaggagg cggccgagaa ggccaagatc gagctgtcct cgctgcagga aaccacgatc
     4201 aacctgccgt tcatcaccgc cacccaagaa gggccgctgc acctcgagct cagcctgtcc
     4261 cgggcgaagt tccaggagct gaccgccgat ctgctcgacg cttgcaaagg cccgtttgag
     4321 caggcgatcc gcgactcggg actaacgagc gacgggattc accgcgtgat cctcgtcggc
     4381 ggctcgacgc gcatgccggc cgttcagggt gtcgtcaagg acgtgaccgg caaggaaggc
     4441 gacaagggcg tcaacccgga cgaggcggtc gccgtcggtg ccgcgatcca agccggggtg
     4501 ctcaagggtg acgtcaaaga cgtcttgctc ctcgacgtca cgccgctgtc gctcggcatc
     4561 gagacgaagg gtcagatcat gacccgactc atcgagcgga acacgacgat cccgacgaag
     4621 cgctccgagg tcttcaccac cgcggacgac aaccagccca gcgtggagat caaggtcctg
     4681 cagggtgaga gcgacatggc gtaccgcaac aagatgctcg gcaccttcca gctggtgggt
     4741 atgccaccgg ccccgcgggg tgtaccgcag atcgaggtca cgttcgacat tgacgccaac
     4801 ggcatcgtgc acgtgagcgc gaaggacctc gggaccggca aggaacaggc catgaccatc
     4861 accgggggca ccgccctcgg caaggaagag atcgaccgga tggtcaaaga ggccgaagcc
     4921 cacgccgagg aggaccgcaa gcgccacgac gaggccgaga cccgcaacaa cgccgactcg
     4981 ctcgtcttcc agaccgagaa gctcctgaag gagcaggccg acaaggtctc cgccgaggac
     5041 cgcaccaaga tcgaatcggc gctcggggag ctgaagacgg cgatcgccgg cgaggacatc
     5101 gacgccgtcc gccagaagca cgaagcgttg ctcaccgcga gccaggagtt cgcgcaacgc
     5161 ctctaccagg cggcccaggc acaacagtcc gcgtccggca cctccggtga gggcgccgcg
     5221 tccgataccg cccagccatc cgacgatgag gtcgccgacg ccgagattgt cgacgagggc
     5281 cacgacggtc acgagaaaag cgcgtgatgc ccgaagaatc gctcggcggg gacgccgaac
//
LOCUS       DATCCH010000451         3902 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_30654, whole genome shotgun sequence.
ACCESSION   DATCCH010000451 DATCCH010000000
VERSION     DATCCH010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3902
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30654"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..754
                     /gene="purQ"
                     /locus_tag="VIJ44_08705"
     CDS             71..754
                     /gene="purQ"
                     /locus_tag="VIJ44_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEY5166017.1"
                     /translation="MTARVAIVVFPGTNCELDVARAVAGLGGEPELVFHTETTLGNAD
                     AVVVPGGFAHGDYLRTGALARFSPVMAAVAAHAASGGPVVGICNGFQVLTEAALLPGA
                     LQKNAGLKFRCETVACRVDSTRSVLTSRARAGSVLRLPINHFEGAYICDPATLERLRA
                     EDGIVLRYVENPNGSLDGIAGITNAAGNVVGLMPHPERASDALLGSADGIVLLESLLV
                     AAGERAVAA"
     gene            complement(776..1180)
                     /locus_tag="VIJ44_08710"
     CDS             complement(776..1180)
                     /locus_tag="VIJ44_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5166018.1"
                     /translation="MAPKQLTTEHKEAMAAGRTEGRAVKAYLDALAEHRPRRGRQRTA
                     ESIRQRLTAITAELGAATSLRRLQLIQERRDLESELEKKAGPAADLAALEADFAKVAK
                     AYAERKGITYATWREMGISSEVLTRARITRGS"
     gene            1267..3507
                     /gene="purL"
                     /locus_tag="VIJ44_08715"
     CDS             1267..3507
                     /gene="purL"
                     /locus_tag="VIJ44_08715"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEY5166019.1"
                     /translation="MSTEPLHRTLGLSDDELVAITDLLGRPPSDLELAMYAVMWSEHC
                     SYKSSKLHLGRFPTRAPWVLVGPGEGAGVIDVGDGLAVALRIESHNHPSAVEPYQGAA
                     TGVGGIIRDVFSMGARPIALMDSLRFGPLDDVRTRYLFESVVSGISGYGNAVGVPTVG
                     GEAVFDECYRDNPLVNVLCLGLVSQDRMVLARAGDAGNLAVLLGASTGRDGIGGASVL
                     ASAGFDEGSDSKRPSVQVGDPFEEKRLIEACLELLAAGLAVGVQDLGAGGLSCAASET
                     AASSGAGMDVDVGRVARREPQMTPVEVMTSESQERMLAIVDPARLDEVLRLCARWDVR
                     ATVVGRVTDTQRFRVYDGVLDDDDRPAPALADVPIASLGDGPVYSRPVERPAAQDALV
                     AADPWPTLAASFPGDADLGAELLALLATPTIADKSWVWRQYDHQLFLNTVVGPGGDAS
                     VLRLPGTERGLAFSTDGKARFCRLDPNTGGRLAVLEAARNVACAGAEPRALVNCLNFG
                     NPEHPDVMWQFQQTVEGISEACRALTIPVVGGNVSFYNESRGANIDPTPIVGVLGVIE
                     RLDSVPPPAASGPGQRIVLLGETLVELGGSEWAARHALRGGAAPSADLTAGRRLHELV
                     RDLVIERRVRGVHDCADGGVAVTLTEMAIAGGCGFRVAPEAGMAPAAWCFAESASRIV
                     LAVDPAESPTVMRRASEAGVPATELGETSGARLTIDGALDVALTDAERAWREALPHAL
                     GASPAH"
     gene            complement(3518..>3902)
                     /locus_tag="VIJ44_08720"
     CDS             complement(3518..>3902)
                     /locus_tag="VIJ44_08720"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF013730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEY5166020.1"
                     /translation="GKTTIGVAVVDELRSRGARAALVDAPDVVAHLSSDEPSDALGWL
                     ARLLTATGAIVVIASDDPRRAARDRLRAEVPGFVEIFVDGGPGRDHYEEPVAPELRVP
                     THDRSPEASVAQVVAWLERNVVGPL"
BASE COUNT          518 a         1382 c         1364 g          638 t
ORIGIN      
        1 ctgctcgcca acccggtgat cgaggcgtac gacatcgagc tcaccgagct cgtctcggcc
       61 cggtgaaccg atgactgccc gcgtcgccat cgtggtgttc cccggcacga actgcgagct
      121 cgacgtcgcc cgagccgtgg ccggactcgg cggcgaaccc gagctggtgt tccacaccga
      181 gaccaccctc gggaacgccg acgccgtggt ggtccccgga ggattcgccc acggcgacta
      241 cctccgcacc ggggcgctag cccggttctc gccggtcatg gccgcggtcg ccgcgcatgc
      301 cgcctcgggc ggaccagtgg tggggatctg caacggcttc caggttctca ccgaggccgc
      361 cctgctcccc ggggccctcc agaagaacgc cggcctcaag ttccggtgcg agaccgtggc
      421 gtgtcgcgtg gatagcaccc gctccgtgtt gaccagccgg gcccgggccg gttcggtcct
      481 ccggcttccg atcaaccact tcgagggggc gtacatctgc gacccggcca cactcgagcg
      541 gctccgcgcc gaggacggca tcgtgttgcg ctacgtcgag aatcccaacg ggagtctcga
      601 cggcatcgcc gggatcacga acgctgccgg caacgtcgtc ggcctcatgc cccatcccga
      661 gcgcgcctcc gacgcgctcc tgggctccgc cgacggcatc gtgctgctcg agtcgctgct
      721 cgtcgcggcc ggcgagcgag ccgtcgccgc ctgacgccga actcccgagg ggggctcagc
      781 tccctcgggt gattctggct cgggtgagga cctcgctgct gattcccatc tcccgccacg
      841 tcgcgtaggt gatccctttg cgctcggcgt acgccttcgc caccttggcg aagtccgcct
      901 cgagcgccgc gagatccgcc gccggccccg ccttcttctc cagctcggac tcgaggtcgc
      961 gacgctcctg gatcagttgg agtcgccgca gcgacgttgc cgcgccgagc tcggcggtga
     1021 tggcggtcag ccgttgtcgg atcgactcag ccgtgcgctg ccggccgcgg cgtggtcgat
     1081 gctcagccag ggcgtccaag taggccttca cggcgcgccc ctcggtccgg cccgcggcca
     1141 tcgcctcttt gtgctcggtc gtcagctgtt tcggtgccat ttgccatcct cggagagacc
     1201 catttcccct attctgtcac ttggcatttg tgtaaatcga gccgccggta ggctccgaag
     1261 gtggccgtga gcaccgagcc tctgcaccgg acgcttggcc tcagcgacga cgagctggtc
     1321 gccatcaccg atctgctggg tcgccctcct tcggatctcg agctggcgat gtatgccgtc
     1381 atgtggtccg agcactgctc gtacaagtcg tcgaagctgc atctcggtcg cttcccgacc
     1441 cgagccccat gggtgctggt cggccccggt gaaggcgccg gtgtcatcga cgtcggggac
     1501 gggctggccg tcgcgttgcg gatcgagagt cacaaccatc cgtcggcggt ggagccgtac
     1561 cagggtgcgg cgactggcgt gggcgggatc atccgagacg tcttctccat gggggcgcgc
     1621 ccgatcgcgt tgatggactc gctgcgcttc ggtccactcg atgatgtccg gacccggtat
     1681 ctcttcgaga gcgtcgtgtc cgggatcagc ggttacggca acgcggttgg tgtcccgacg
     1741 gtgggtgggg aagcggtctt cgacgagtgc taccgcgaca atccactggt caacgtcttg
     1801 tgtctcggcc tggtgtctca ggaccgaatg gttctggccc gcgccggcga cgccgggaac
     1861 cttgcggtgc tgctcggtgc gtcgacggga cgcgacggga tcggcggggc cagcgtgctc
     1921 gcgtcggcgg gtttcgacga gggctctgat agcaaacgac cctcggtgca ggtcggcgac
     1981 ccgttcgagg agaagcgcct catcgaggcg tgcctcgagt tgctcgccgc ggggctggcc
     2041 gtcggcgtcc aagatctcgg cgccggtggt ctgtcgtgcg cggcgtcgga gacagcggcg
     2101 agcagcggtg ccggaatgga tgtcgatgtg ggacgggtcg cgcgccggga gccccagatg
     2161 accccggtcg aggtcatgac gtcggagagt caggagcgga tgctcgcgat cgtcgacccg
     2221 gcacgacttg acgaagtgct ccgcctgtgc gcccgctggg atgtccgtgc caccgtcgtg
     2281 ggacgcgtca cggacaccca acggttccgg gtctacgacg gcgtgctcga tgacgacgat
     2341 cggcccgcac ccgctctcgc cgacgttccg atcgccagcc tcggtgacgg cccggtctac
     2401 tcgcggccgg tcgagcggcc cgcggctcag gacgcgctcg tcgccgctga cccctggccc
     2461 accctcgccg catcgtttcc gggcgacgcc gacctcggcg cggagctcct ggcgctgctc
     2521 gccacgccca cgatcgccga caagtcgtgg gtgtggcgcc aatatgacca tcagctcttc
     2581 ctcaacaccg tggtgggccc cggcggtgac gccagtgtgc tgcggctccc cggcacggag
     2641 cggggcctcg cgttctcgac cgacggtaag gcccggttct gtcgcctcga ccccaacact
     2701 ggcggtcgcc tcgcggtgct cgaagccgct cgcaacgtgg cgtgcgccgg cgccgagccg
     2761 cgggcgctcg tgaactgcct caacttcggc aaccccgagc accccgacgt gatgtggcag
     2821 ttccagcaga ccgtcgaggg catcagcgag gcgtgccgcg cgctgacgat cccggtcgtc
     2881 ggcgggaacg tgagcttcta caacgagtcg cgtggtgcca acatcgaccc gaccccgatc
     2941 gtcggggtac tcggcgtcat cgaacggctc gactcggtcc cgccgccggc ggcctccggc
     3001 ccggggcaac ggatcgtgct cctcggtgag acgctcgtcg agctcggagg ctccgaatgg
     3061 gcggcacggc acgctcttcg cggcggcgcc gccccctcgg cggacctcac cgcgggccgc
     3121 cggctccacg aacttgtccg cgacctggtg atcgagcgcc gagtgcgggg ggtgcacgac
     3181 tgcgccgacg gcggcgtggc ggtgacgctc accgagatgg cgatcgccgg cggctgcggc
     3241 ttccgggtcg cacccgaggc cggtatggcg ccagcggcct ggtgcttcgc cgagtccgcg
     3301 tcgcggatcg tcctcgcggt cgatcccgcc gagagcccga cggtcatgcg ccgagcgagc
     3361 gaggcggggg ttccggcaac cgagctcggc gagaccagcg gtgctcgact cacgatcgac
     3421 ggagccctgg atgtcgcgtt gaccgacgcg gagcgggctt ggcgcgaggc gctgccgcac
     3481 gcgctcggtg catcacccgc gcactaatgg gtgcggatca gagcgggccg accacgttgc
     3541 gttccagcca cgcgacgacc tgcgccaccg acgcctcagg cgaacgatcg tgggtcggga
     3601 cccgcaactc gggtgcgact ggctcctcgt agtgatctcg ccccggtccc ccgtcgacga
     3661 agatctccac gaagcctggc acctcggctc ggagccggtc ccgggcggca cggcggggat
     3721 cgtcggaggc gatgaccacg atcgcgccgg tcgcggtgag gaggcgggcg agccatccga
     3781 gcgcgtcgct cggctcgtcg gacgacaggt gggcgaccac gtccggcgcg tcgacgaggg
     3841 cggcccgagc gccccggctt cgcagctcgt cgacgacagc caccccgatg gtcgtcttgc
     3901 cc
//
LOCUS       DATCCH010000452         3642 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_73071, whole genome shotgun sequence.
ACCESSION   DATCCH010000452 DATCCH010000000
VERSION     DATCCH010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73071"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /gene="atpH"
                     /locus_tag="VIJ44_08725"
     CDS             <1..444
                     /gene="atpH"
                     /locus_tag="VIJ44_08725"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HEY5166021.1"
                     /translation="DLFRFARTVQSSDDLRMSLSDPKLPINRRLALIDELLDGKALAA
                     STALISMVVAAGHAGDLPAIVERFVELAAAERERELAEVRSAIPLDQAQTKRLADALS
                     HATGKRVEVKVIVDPSVLGGIVARVGDTVIDGTIRHRLEQLKEQV"
     gene            488..2098
                     /gene="atpA"
                     /locus_tag="VIJ44_08730"
     CDS             488..2098
                     /gene="atpA"
                     /locus_tag="VIJ44_08730"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEY5166022.1"
                     /translation="MPELTINADEIADALRRHVDTFVPSVDQARVGRVLEVGDGIARV
                     AGLPDIAVNEVLEFTGGTLGLALNLDEDSIGAVILGDAHEVEEGQAVKATGRILSVPV
                     GNSLIGRVVDALGQPIDGKGPLVAEAQRRLEVQAPGIVYRKPVHEPLQTGIKAIDAMT
                     PIGRGQRELIIGDRKTGKTTVAIDTILNQRGSGVKCIYVAVGQKGTSVAQTVATLESR
                     GAMEYTVVVSAPASEEAVFKYLAPYAGCAMGQHWMEHGEAALIVYDDLSKQAEAYRQL
                     SLLLRRPPGREAYPGDVFYLHSRLLERAAKLNDEEGAGSLTALPIIETKAGDVSAYIP
                     TNVISITDGQIYLVDDLFKSGVRPAVDVGISVSRVGGAAQVKAMKSVAGTLKIDLAQF
                     RDLEAFATFGSELDAVSRAQLDRGQRLVELLKQPLNSPMPVEEQVIVIFAGTQGLVDD
                     IPVDQVRRFEQDLLDWFRARHSGLLSEIRLSGALPDGDAILEAITNFKTEFAATIQAE
                     ARAGDADPMATDAEAPGDPASDKTLQTE"
     gene            2188..3135
                     /locus_tag="VIJ44_08735"
     CDS             2188..3135
                     /locus_tag="VIJ44_08735"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019629367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEY5166023.1"
                     /translation="MASGQERILRRRIKSVESTKKITRAMELIAASRIVKAQARVHAA
                     VPYSDTITEVMHDLAAAGAGSDSPLLNPRSDVGKVAHIVVTADRGLCGAYNSTVIRAA
                     EGSMREQSAQGRDYGLIVVGRKAEAYFRFRHYRIDAAFTGFSEQPTYEDARVIARAVE
                     GPFLGGEYGLVELIYTRFISVGSQEVVVRPLMPLDPETIAASGTRSGSDDDGVGPAYE
                     FEPSPDSILEQLLPRYVEARTYAALLNAAASEQAARQRAMKAATDNADDLIVRLTRVM
                     NRARQDAITTEIMEIVGGAEALRDEEVEPKSADFLVPEH"
     gene            3151..>3642
                     /locus_tag="VIJ44_08740"
     CDS             3151..>3642
                     /locus_tag="VIJ44_08740"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440627.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEY5166024.1"
                     /translation="MTVTADSATELKEGRVVAIAGPVVDVEFPPDTLPEINTAVEMDI
                     ELEGDTITVVAEVAQQIGEGRVRVICMKPTDGLKRGTVVRNTGRGITMPVGDGVLGHV
                     FNVIGEPLDTTADQITGIEDRWEIRRDAPPFDQLEPKSQMFETGIKVIDLLEPYVEGG
                     KIGL"
BASE COUNT          632 a         1307 c         1186 g          517 t
ORIGIN      
        1 gacctgttcc gcttcgcgcg cacggtgcag agctccgacg accttcggat gtcgctcagc
       61 gacccgaagc tccccatcaa ccggcggctc gcgctgatcg acgagctcct cgacggcaag
      121 gccctggccg ccagcaccgc gctgatctcg atggtcgtcg ccgccggcca cgccggcgac
      181 ctgcccgcca tcgtcgaacg gttcgtcgag ctcgccgccg ccgaacgcga acgcgagctg
      241 gccgaggtgc gcagcgccat cccgctcgac caagcccaga ccaagcggct cgccgacgcg
      301 ctcagccacg cgaccgggaa acgagtcgag gtcaaggtga tcgtggaccc gtcggtcctc
      361 ggcgggatcg tcgcccgggt cggcgacacc gtgatcgacg gcacgatccg tcaccgtctc
      421 gagcagctca aggagcaggt ctgatgcgtg cgccgatcca tcccggcttc atcacggagg
      481 aggcgtcatg ccggaactga ccatcaacgc cgacgagatc gcggacgccc tccgtcgcca
      541 cgtcgacacg ttcgtgccca gcgtcgacca ggctcgggtt ggccgagtcc tcgaggtcgg
      601 cgacggcatc gcccgggtgg ccggactacc cgacatcgcg gtcaacgagg tgctggagtt
      661 caccggtggc acgctcggcc tcgccctgaa cctcgacgag gactcgattg gcgcggtcat
      721 cctcggcgac gcccacgaag tcgaggaagg ccaggcggtg aaggccaccg gccggatcct
      781 ctcggtgccc gtcggcaact cgctcatcgg acgggtggtc gacgccctcg gccaacccat
      841 cgacgggaaa gggcccctcg tcgctgaggc ccaacgccgc ctcgaagtgc aggcccccgg
      901 gatcgtgtac cgcaagccgg tccacgagcc gcttcagacc ggcatcaagg ccatcgacgc
      961 catgaccccg atcgggcgcg gccagcgcga gctcatcatc ggtgaccgca agaccggcaa
     1021 gacgaccgtc gccatcgaca cgatcctcaa ccagcgcggc agcggcgtga agtgcatcta
     1081 cgtcgccgtc ggtcagaagg ggacgtccgt cgcgcagacc gtcgcgaccc tcgaatcccg
     1141 gggcgcgatg gaatacaccg tcgtcgtcag cgcgcccgcc tccgaagagg cggtgttcaa
     1201 gtacctggcg ccctacgccg gttgcgccat gggccagcac tggatggaac acggcgaggc
     1261 cgcgctcatc gtctacgacg acctctccaa gcaagccgag gcctaccggc agctgtccct
     1321 gctgctccgc cgcccgccgg gccgtgaggc gtaccccggc gacgtcttct acctccacag
     1381 ccggctgctc gagcgggccg cgaagctcaa cgacgaggaa ggcgccgggt cgctgacggc
     1441 gctgcccatc atcgagacga aggccggtga cgtgtcggcc tacatcccga cgaacgtcat
     1501 ctcgatcacc gacggtcaga tctatctcgt cgacgacctc ttcaagtcgg gggtccgtcc
     1561 cgcggttgac gtcggcatct cggtgtcccg ggtcgggggc gcggcgcagg tcaaggcgat
     1621 gaagagcgtc gccggcacgc tcaagatcga cctcgcccag ttccgcgacc tcgaggcgtt
     1681 cgcgaccttc ggctccgagc tcgacgcggt gtcgcgcgca caactcgatc gcgggcaacg
     1741 gctcgtggag ctgctcaagc agccactgaa ctccccgatg cccgtcgagg agcaggtcat
     1801 cgtcatcttc gccggcaccc aaggccttgt cgacgacatc ccggtcgacc aggtgcggcg
     1861 cttcgagcag gatctgctcg actggttccg agcccgtcac agcgggctgc tctcagagat
     1921 ccggctctcg ggcgcccttc ccgacggcga tgcgatcctg gaagcgatca cgaacttcaa
     1981 gaccgagttc gcggcgacga tccaggccga ggctcgagcg ggcgacgccg acccgatggc
     2041 cacggacgcg gaggccccgg gggacccggc gtcggacaag accctccaga ccgaatagcg
     2101 atgctggacc ggacgccccc acccgttccc atgacgcgac ggcgtcgccc cggtggcgcg
     2161 gcgcgccgtg cgcccgggcg gcgctagatg gcgagcggtc aggagcggat cctccgccgg
     2221 aggatcaaga gcgtcgagtc gacgaagaag atcacccggg cgatggagct gatcgccgcg
     2281 agccggatcg tgaaggccca ggctcgggtt cacgccgcgg tgccgtactc cgacaccatc
     2341 accgaggtca tgcacgacct ggccgcagcc ggtgcgggct ccgacagccc gctgctcaac
     2401 ccgcgtagcg acgtgggcaa ggtcgcgcac atcgtcgtca cggctgaccg tggactctgc
     2461 ggggcgtaca actcgacggt gatccgcgcc gcggaagggt cgatgcgcga acagagcgcc
     2521 caaggtcgcg actacgggct catcgtcgtc ggacgcaaag ccgaggccta cttccgcttc
     2581 cgtcactacc gcatcgatgc tgcgttcacc ggcttctccg agcagcccac ctacgaagac
     2641 gcccgggtca tcgcccgggc cgtcgaaggg cccttcctcg gaggcgagta cgggctcgtc
     2701 gagctcatct acacccgttt catctcggtc ggctcccaag aggtcgtggt gcgccccttg
     2761 atgcccctcg acccggagac gatcgccgcg agcggcaccc gatccggatc cgacgacgac
     2821 ggggtcggtc ccgcctacga gttcgagccg tcccccgact cgatcctcga gcagctcctg
     2881 ccccgctacg tcgaggcgcg cacgtacgcg gcgttgctga acgccgccgc atccgagcaa
     2941 gcggctcgcc aacgagccat gaaggccgcg accgacaacg cggatgacct catcgtccgg
     3001 ctgaccaggg tgatgaaccg ggcccgtcag gacgccatca ccaccgagat catggagatc
     3061 gtcggtggtg ccgaggcgtt gcgcgacgaa gaggtcgaac ccaagtccgc cgatttcctc
     3121 gtccccgagc attagtccag gagtctgagc atgaccgtca ccgctgattc ggccaccgag
     3181 ctcaaggagg gccgcgtcgt cgccatcgcc gggccggttg tcgacgtcga gttcccaccc
     3241 gacaccctgc ccgagatcaa caccgcggtc gagatggaca tcgagctcga aggcgacacg
     3301 atcaccgtcg tcgccgaggt tgcccagcag atcggcgaag gccgggtgcg ggtcatctgc
     3361 atgaagccca ccgacgggct caagcgaggg acggtggtcc gcaacaccgg ccgcggcatc
     3421 accatgccgg tcggcgacgg ggtgctcggc cacgtcttca acgtgatcgg cgagccgctc
     3481 gacaccaccg cggaccagat caccggcatc gaggaccggt gggagatccg ccgcgacgcg
     3541 ccaccgttcg atcagctcga gccgaagtct cagatgttcg agaccggcat caaggtcatt
     3601 gacctgctcg agccgtacgt cgaaggcggc aagatcgggc tc
//
LOCUS       DATCCH010000453         2513 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_31122, whole genome shotgun sequence.
ACCESSION   DATCCH010000453 DATCCH010000000
VERSION     DATCCH010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31122"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="VIJ44_08745"
     CDS             <1..760
                     /locus_tag="VIJ44_08745"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY5166025.1"
                     /translation="GDEWVVNGQKVWNSFAHFADWGILLARTDWDVPKHRGISFFLVD
                     MRTPGVEARPLRQITGVAHFNETFLTDVSIPSDQLLGAVNGGWGVAQTTLMNERALIS
                     GGGSGLGFRDYVEVARHYRCTQDPEIRQRLAAAYTRFEILKWLGQRAQAAMRSGRPLG
                     PESSVAKLAVSEHTARSGDLALAIEGAHAMLSGRDAYEDGFWQQQFLGQWGIRIGGGT
                     EQIQRNILGERVLGLPSEPRPDKTEPFRDIPKNS"
     gene            924..>2513
                     /locus_tag="VIJ44_08750"
     CDS             924..>2513
                     /locus_tag="VIJ44_08750"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY5166026.1"
                     /translation="MKALDGLRVVELGGEIAAPYAAKLLADLGADVVKVEPLTGDPLR
                     RWTPFPDDVDRAGLLFRYLNGGKRSCVVDLDTTAGVAWLRDTLDGADVLVESLGAGRI
                     EALGIDLTTPGPTLRPLAVVRIAPYGQSGPYVGRPTSGLVLQALGGWVSNHGVPEMDP
                     VQVGARMHEYAAGTYAAAAVLTAARTARQGHTTAVVDLAVMECLVGTLAYPNLVMEDL
                     LAAGLTPPQARHFPLPGIVRCRDGWVGINALTGQHFVDACTMFELDEFGSRQPELAEG
                     GPLLDEFYARLEPWLRARDAADIVELSQAFRVPAAPVGDGRMLLDYAQFRERPFFIGE
                     DGVTMPGPPYRLSATPASRRGPAPALGQHAGDEPSATPDPAPGPAADVLPFAGLRVVD
                     LGSFWAGPYCTMYLGALGADVVKVESTRRPDGFRFSGARPEMGVDWYERSGVFAGTNL
                     NKRNVTLELTTEAGRALLRRLVDGADVVLENFSARVVEQFELGYEQLREINPNVIMVR
                     MPGFGLVGPWRDYVGWAMVIEQ"
BASE COUNT          365 a          932 c          836 g          380 t
ORIGIN      
        1 cggcgacgag tgggttgtca acggtcagaa ggtgtggaac tcgttcgcgc atttcgctga
       61 ctggggcatc ctcctcgccc ggacagactg ggatgtcccc aagcaccggg ggatcagctt
      121 cttcctcgtc gacatgcgaa cgcccggtgt cgaagcccgc cccctgcgtc agatcaccgg
      181 cgtcgcgcac ttcaacgaga cctttctcac cgacgtgagc atcccctcgg accagctcct
      241 cggcgcagtc aacggcggat ggggcgtcgc ccagaccacg ctgatgaacg agcgcgcgct
      301 gatcagcggc ggcggttccg gcctcgggtt ccgggactac gtcgaggtcg cccgccacta
      361 ccgctgcacc caggatccgg agatccgcca gcggctcgcc gccgcctaca cgcgcttcga
      421 gatcctcaaa tggctcggtc aacgggccca ggccgcgatg cgatccgggc ggcctctcgg
      481 ccctgagagc tccgtggcca agctcgccgt ctccgagcac accgcccgca gcggcgacct
      541 ggcgctggcg atcgaagggg cgcacgccat gctctcgggc cgcgacgcct acgaagacgg
      601 gttctggcaa cagcagttcc tcggacagtg gggaatccgc atcggcggcg gaaccgagca
      661 gatccagcgc aacatcttgg gtgagcgggt cctcggtctt ccgtcagaac cgcgtccgga
      721 caagaccgag ccgttccgcg acatcccgaa gaacagctga tccgacaccc ctcctgacgt
      781 cgccggcgcg agccgagacg tcgagcgggt cgcagaccac cccgaatacg ctccgcggcc
      841 gtggaccccg acgacaggct gatccgactc ctcgcgatcc gccccacgcg acgccgggtc
      901 gatctccttc tcggcgggct ggggtgaagg ccctcgacgg gctccgagtc gtcgagctcg
      961 gcggcgagat cgccgccccg tacgccgcga agctcctcgc cgacctcggc gccgacgtcg
     1021 tcaaggtcga gcccctgacc ggggatccgt tgcgccgctg gaccccgttc cccgacgacg
     1081 tcgaccgagc cggcttgctc ttccgatacc tgaacggagg caagcgcagc tgcgtcgtcg
     1141 acctcgacac caccgccggc gtggcgtggc tccgcgacac cctcgacggg gccgacgtcc
     1201 tcgtcgagag cctcggcgcc ggccgcatcg aagcgctggg aatcgacctg acgacgccgg
     1261 gtccgacgct ccggcccctg gcggtggtgc gcatcgcccc gtacgggcaa tcgggcccgt
     1321 acgtcggtcg gcccaccagc gggctggtgc tccaagcgct cggcggctgg gtgtcgaatc
     1381 acggggtccc tgaaatggat ccggtgcagg tcggggcccg catgcacgag tacgccgccg
     1441 ggacctacgc cgccgcggcg gtcctgaccg ccgcccggac agctcggcag ggccacacca
     1501 ccgcggtcgt cgacctcgcg gtgatggagt gcctggtcgg gacgctcgcg tacccgaact
     1561 tggtgatgga ggacctcctc gcggcgggat tgacgccacc ccaagcccgc catttccccc
     1621 ttccgggaat cgtccgctgc cgggacgggt gggtcggcat caacgcgttg acgggtcagc
     1681 acttcgttga cgcctgcacg atgttcgagc tcgacgagtt cggctcccgc caaccggagc
     1741 tcgccgaggg gggtccgctg ctcgacgagt tctatgcccg cctcgaaccc tggctccggg
     1801 cccgcgacgc ggccgacatc gtcgagctca gccaggcgtt tcgcgttccc gcggccccgg
     1861 tcggagacgg gcgcatgctg ctggactacg cccagttccg tgaacgcccg ttcttcatcg
     1921 gcgaagacgg cgtcacgatg cccgggcccc cgtaccggct ctctgccacc cccgcctccc
     1981 ggcgcggtcc cgcgcccgcg ctcggtcagc acgccggcga cgagccgtcg gcgactcccg
     2041 atcccgcccc tggccccgcc gccgacgtcc tcccattcgc gggtctccgg gtggtggacc
     2101 tgggttcgtt ctgggccggc ccgtactgca ccatgtacct cggtgcgctc ggcgccgacg
     2161 tcgtcaaagt cgaatcgacc cgacgccccg acgggttccg gttctccggc gcgcgcccgg
     2221 agatgggtgt cgactggtac gagcgcagcg gggtcttcgc gggaacgaac ctgaacaagc
     2281 gcaacgtcac cctcgagctc accaccgagg cgggccgagc gttgctccgc cgcctcgtcg
     2341 acggcgccga cgtggtcctg gagaacttct cggcccgggt cgtcgagcag ttcgagctcg
     2401 gctacgagca gctgcgcgag atcaacccga acgtgatcat ggtgcggatg cccgggttcg
     2461 gcctggtcgg cccgtggcgc gactacgtcg gctgggccat ggtcatcgag cag
//
LOCUS       DATCCH010000454         5355 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_155965, whole genome shotgun sequence.
ACCESSION   DATCCH010000454 DATCCH010000000
VERSION     DATCCH010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5355
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155965"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..554
                     /locus_tag="VIJ44_08755"
     CDS             162..554
                     /locus_tag="VIJ44_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5166027.1"
                     /translation="MAAVTTVTGISVRAFPMTRPEPLRVATPRQSRTSPATFRRRRIV
                     TAVLALGVVVVTGQAGAALGGSSLAAPERRPSLTASHTVVVGPGDTLWGIAHRLAPNA
                     DPRPIVDALTAVYGPGPLTPGETLRLPA"
     gene            607..1092
                     /gene="nrdR"
                     /locus_tag="VIJ44_08760"
     CDS             607..1092
                     /gene="nrdR"
                     /locus_tag="VIJ44_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019631065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEY5166028.1"
                     /translation="MRCPFCLADDDKVVDSRPADDGAAVRRRRECLACGRRFTTQERV
                     VELPLMVVKRSGEREPFERSKLVGGIDRAVAGSPIGPEAVEALAAEIEEEARAGGAEV
                     GSERLGLGVVERLQVLDPVSYLRFASVYQGFESAADFERAVIALRKTTKPKRGTGRAP
                     S"
     gene            1239..4019
                     /locus_tag="VIJ44_08765"
     CDS             1239..4019
                     /locus_tag="VIJ44_08765"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011292572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HEY5166029.1"
                     /translation="MAIAPEQIGIGIRRYFTRPGVHPYDTVEWERRDARIQNYKDGTD
                     AFFQPDVEFPVTWTQNATNIVAQKYFRGTPGTPEREWSLKQVIDRVVDTITAWGMRDG
                     YFVDNQEGAAFRNELKFILLNQRAAFNSPVWFNIGVEGVPNQASACFILAVEDTMDGI
                     LNWYREEGIIFKGGSGSGINLSGIRSSQEHLAGGGTASGPVSFMRGADASAGTIKSGG
                     KTRRAAKMVILNADHPDIEEFIWCKAREERKARALRDAGFDMDLDGVDAYSIQYQNAN
                     NSVRVTDEFMRAVAEDDDWQLKQVTAGQASRTIKARDLMRQISQAAWECADPGMQYDT
                     TINRWHTAPKTGRINASNPCSEYMHLDNSACNLASINLLRYLNDDDSFDTEGFAHTVE
                     VMFTAQEILVGNADYPTEKIADTSGRFRQLGLGYANLGALLMAQGLPYDSDAGRAWTS
                     AITALMTGHAYATSARTAGRMGPFAGYADNADAMLNVLRMHRGEAAKINEELVPPELL
                     GAAQRSWDEAVEVGEAHGVRNSQATVLAPTGTIALMMDCDTTGIEPDLALTKAKKLVG
                     GGTMLIVNQTVPRALKKLGYSSEECAAIVKHIDEHKSIIGSPQFKAEHLPVFACSMGD
                     NTIHYMGHIRMMAAAQPFISGAISKTANVPESATVEEIEQLYLDSWKLGLKAVALYRD
                     NCKVAQPLATQKKAGSSDDGGLSREVERIVETVIVQRPVRQKLPRTRTSKTFSFRVAD
                     CHGYATVGEFEDGRPGEVFLKVAKQGSTLSGIMDAFAISVSLGLQYGVPLRAFVEKFT
                     NMRFEPAGMTDDPDIRFASSLVDYIFRRLAVEYLTLEERTDLGIFTIHERTQPTLPGV
                     EEATTPSHPSVDVLPPEDRAPSSSAVPPEVPFLPVDREVEAMLCYVCGDIMQRAGSCY
                     ACPSCGATSGCS"
     gene            complement(4021..4494)
                     /locus_tag="VIJ44_08770"
     CDS             complement(4021..4494)
                     /locus_tag="VIJ44_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY5166030.1"
                     /translation="MAEQVSATREIGAPAKQVWAMVSDVTRMGEWSPETETAAWLRGA
                     AGPQPGARFRGMNRNGKKKWNTVATIVGAEPGRLLSFRVTAAGFKVAEWRYTFESTAT
                     GCRVTESWLDQRGRIARALGKPVSGVEDRATHNRAGMEQTLERLKAVAESSPVPS"
     gene            4716..>5355
                     /locus_tag="VIJ44_08775"
     CDS             4716..>5355
                     /locus_tag="VIJ44_08775"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY5166031.1"
                     /translation="MRIDRSIRRALSVFDADRLHWLDTAAALGPLAGLRFGPTTAWVL
                     SDADAARTMLVTDGDSWTRPPTARVPIRLGVGENLFTQTDKAWAFLQPQIAPPFRKRA
                     LDARLAELDGLVDQEVASLPMGERLDLDAAMGGIALIVAAWVLLGEHLARSRADELAD
                     HQRQIVSWVGHRIGRATAAVPLALGAPGRAMREHRRALEAYADEIVARAQQRG"
BASE COUNT          933 a         1883 c         1684 g          855 t
ORIGIN      
        1 cggaacggac ggcccggggc ggggggttga cggcgaacga gtgttcgtgc ttgactacga
       61 acaagcgttc gtcgtccgcc cgccgggcag gctcgaacgc cgggagagcg tcacaccctc
      121 gtgttctgct cctctcgatg accacgaaac caggaggagg tatggccgcc gtcacgaccg
      181 tcaccggcat cagcgtccgg gcgtttccca tgacccgccc ggagcccttg cgcgtagcca
      241 ccccgcgcca gagccggacg tcccccgcga cctttcgacg ccgccgcatt gtcaccgcgg
      301 tgctcgcgct cggggtcgtg gtcgtgacgg ggcaggcggg tgccgcgctc gggggctcat
      361 ccctcgcagc tcccgagcgc cgccccagcc tcaccgcctc gcacaccgtt gtcgtaggac
      421 ctggagacac cctgtggggc atcgcccacc ggttggcccc gaacgccgac cctcggccca
      481 tcgtcgacgc cctcaccgcg gtctacggcc ccgggccgct caccccgggc gaaaccctcc
      541 gcctcccggc gtgaagcacg ccgcagcccc atcgcgcttg aggcgggaca gcgttaccgt
      601 gaccgggtgc gctgcccgtt ctgtctggcc gacgacgaca aggttgttga ttcccgtccc
      661 gccgacgacg gtgccgcggt ccgccgccgg cgcgagtgtc tcgcctgcgg acgccggttc
      721 acgacccagg agcgtgtcgt cgagcttccg ctcatggtgg tgaaacgttc cggggaacgt
      781 gagccgttcg agcgctccaa gctcgtcgga ggcatcgacc gggccgtcgc cggcagcccc
      841 atcggacccg aagccgtcga agccctcgca gccgagatcg aggaagaagc ccgcgccggt
      901 ggcgccgagg tcgggagcga acgcctgggc ctcggcgtcg tggagcgcct ccaggtcctc
      961 gacccggtct cgtatctgcg cttcgcctcc gtctaccaag gcttcgagtc cgccgccgac
     1021 ttcgaacgag cggtgatcgc gctgcgaaag acgacgaagc ccaagcgggg gaccggccga
     1081 gcccccagtt gagatattcg ccctccacgg ttgactcggc gtagttccag tgatgtaatt
     1141 cacgccgcat ctcgtgttgc taatatcatc aaccacaaca tcttgtggtg agcgggctcg
     1201 agacggccga gagtcggccg gagggggagg acacacccat ggccatagcg ccggagcaga
     1261 tcggcatcgg gatccgccgg tacttcaccc gtccgggcgt ccacccgtac gacaccgtcg
     1321 aatgggaacg acgagacgcc cggatccaga actacaagga cggcaccgac gccttcttcc
     1381 aacccgacgt cgagttcccc gtcacctgga cccagaacgc caccaacatc gtggcccaga
     1441 agtacttccg agggacgccc gggacacccg aacgtgagtg gtccctgaag caggtcatcg
     1501 accgggtcgt cgacaccatc accgcctggg gcatgcgcga cggctacttc gtcgacaacc
     1561 aggaaggcgc ggccttccgc aacgagctca agttcatcct gttgaaccaa cgcgccgcgt
     1621 tcaacagccc ggtctggttc aacatcggtg tcgaaggcgt ccccaaccaa gcgagtgcct
     1681 gcttcatcct cgcggtcgaa gacaccatgg acgggatcct caactggtac cgcgaagaag
     1741 gcatcatctt caaaggcggc tccggttcgg gaatcaacct ttccggcatc cgatcctccc
     1801 aggagcacct cgccggtggc ggtaccgcca gcggtcccgt cagcttcatg agaggtgccg
     1861 acgcctcggc gggcaccatc aaatcgggtg gcaagacgcg tcgcgccgcg aagatggtca
     1921 tcctcaacgc cgatcatccc gacatcgaag agttcatctg gtgcaaggct cgcgaagagc
     1981 gcaaggcccg cgcccttcgg gacgccggct tcgacatgga cctcgatggc gtcgacgcct
     2041 actcgatcca gtaccagaac gcgaacaact cggttcgtgt caccgacgag ttcatgcgcg
     2101 ccgtcgccga ggacgacgac tggcagctca agcaggtgac agccggccag gcttcccgga
     2161 ccatcaaggc ccgggacctg atgcgccaga tctctcaagc cgcttgggag tgtgccgatc
     2221 ccgggatgca gtacgacacc acgatcaacc gttggcatac cgctccgaag acgggtcgga
     2281 tcaacgccag caacccctgc tcggagtaca tgcacctcga caactccgcg tgcaacctgg
     2341 ccagcatcaa cctcctcaga tatctcaacg acgacgactc gttcgacacc gaaggttttg
     2401 cgcacaccgt cgaggtgatg ttcaccgcgc aggagattct ggtcggcaac gctgactacc
     2461 cgacggagaa gatcgccgac accagcggcc gattccgcca gctcgggctc ggctacgcga
     2521 acctcggcgc cttgctgatg gcccagggtc tcccgtatga ctccgacgcc gggcgagcct
     2581 ggacctcggc gatcactgcg ttgatgaccg gccatgccta cgcgaccagc gcgcggaccg
     2641 cgggccgaat gggtcccttc gccggttacg ccgacaacgc agacgccatg ctcaacgtgc
     2701 ttcgcatgca ccgaggcgaa gccgccaaga tcaacgagga gctcgtgccc cccgagctcc
     2761 tcggcgccgc gcagcgttcg tgggacgagg ccgtcgaggt cggcgaagcg cacggcgtcc
     2821 gcaactcaca ggccaccgtg ctcgccccca cgggaacgat cgcgctcatg atggactgtg
     2881 acaccaccgg catcgagccc gacctggctt tgacgaaggc caagaagctc gtcggtggcg
     2941 gcacgatgct gatcgtgaac cagacggtcc cacgcgccct caagaagctc ggttactcct
     3001 ccgaagagtg cgctgcgatc gtcaagcaca tcgacgagca caagtcgatc atcggcagtc
     3061 cccagttcaa ggccgagcac ctaccggtct tcgcgtgctc gatgggcgac aacaccattc
     3121 actacatggg ccacatccgg atgatggccg cagcccagcc gttcatcagt ggcgcgatct
     3181 ccaagaccgc caatgtgcct gaatcggcga cggtcgagga gatcgagcag ctgtacctcg
     3241 attcctggaa gttgggcctc aaagcggtcg ccctctaccg cgataactgc aaggtcgcgc
     3301 agcccctcgc cacccagaag aaggcaggtt cctcagacga cggtgggctg tcccgcgaag
     3361 tggagcgaat cgtcgagacc gtgatcgtgc aacgcccggt ccgccagaag ctgccgcgaa
     3421 cacggacgtc gaagaccttc tcatttcggg tcgcggactg ccacggctac gcgaccgtcg
     3481 gcgaattcga ggacgggcgc ccaggcgagg tgttcctcaa ggtcgccaag caaggctcca
     3541 cgttgtccgg gatcatggat gcgttcgcga tctcggtcag cctcggcctc cagtacgggg
     3601 ttcctctgcg ggcgttcgtg gagaaattca ccaacatgcg gttcgagccg gcaggcatga
     3661 ccgacgatcc cgacattcgt ttcgcgagca gcctggtcga ctacatcttc cggcgcctgg
     3721 ccgtcgagta cctcacgctc gaggagcgca ccgacctcgg gatcttcacg attcacgagc
     3781 gcacccagcc gaccctgccc ggtgtcgaag aggcaacgac tccaagtcac ccttcggtcg
     3841 acgttctccc ccccgaggac cgggcgccat cttcgagcgc cgttccgccc gaagtgccgt
     3901 ttcttccagt agaccgggaa gtcgaggcga tgctctgtta cgtctgcgga gacatcatgc
     3961 agcgagcagg ctcgtgctac gcgtgcccaa gctgcggagc gacgagcggc tgctcgtgag
     4021 tcagctcggg acgggagagg actccgcgac ggccttgagg cgctcgaggg tctgttccat
     4081 ccccgcccgg ttgtgcgtgg cgcggtcctc gaccccgctc accggcttgc cgagtgccct
     4141 cgcgatgcgg ccgcgctggt cgagccaact ctcggtgaca cgacatccgg tcgcggtcga
     4201 ctcgaacgtg tagcgccact cggcgacctt gaagcccgcc gcggtgacgc ggaaggacag
     4261 cagccgaccg ggttcagcgc cgacgatcgt cgccacggtg ttccatttct tcttgccgtt
     4321 gcggttcatg ccgcggaacc gcgccccggg ctgcggaccc gccgcgcccc gtaaccaggc
     4381 ggccgtctcg gtctcgggcg accactcgcc cattcgggtc acgtcggaga ccatcgccca
     4441 tacctgcttc gccggcgcgc cgatctctcg ggtcgcgctc acctgttcag ccatgggccg
     4501 caccttagcg acgcgggtcg aatcaacgaa cccatccgct caccacagcc ccgtccaccg
     4561 gtgggcgaag ctggcccacc gcgcgcggcc ggtgacgacg ttcacctccg gtcgggtgcg
     4621 ctctgtccga tacaggatcg ccgtcggtcc ggggcgagac tgtcgactag ctgctctggt
     4681 cgaacagaag gggcgtcatg aggagtgcgc cgaccgtgcg gatcgaccgt tcgatccgcc
     4741 gggctctgag cgtgttcgat gctgaccgac tgcactggct cgacacagcg gcggcgctcg
     4801 gaccccttgc cgggctgcgc ttcgggccca ccaccgcgtg ggtcctcagc gacgctgacg
     4861 ccgctcgaac gatgctcgtc accgacggtg actcctggac gcgaccacca accgcccggg
     4921 tgccgatccg gctcggtgtg ggagagaacc tcttcactca gaccgacaag gcgtgggcgt
     4981 tcctgcagcc ccagatcgca cctccgttcc gcaagcgtgc cctcgacgca cgtctcgccg
     5041 agctcgacgg gctcgtcgac caggaagtgg cttcgctgcc gatgggtgag cggctcgatc
     5101 tcgatgccgc gatgggcggc attgcgctca tcgtggctgc ttgggtgctc ttgggcgagc
     5161 acctcgcccg cagccgcgcc gacgagctgg cggaccatca gcgacagatc gtcagctggg
     5221 ttggtcatcg aatcggtcga gcgaccgccg cggtgccgtt ggcattgggc gcgcctggcc
     5281 gcgccatgcg cgagcaccgg agagcgctgg aggcctatgc cgacgaaatc gtggcccgtg
     5341 cccagcagcg cggac
//
LOCUS       DATCCH010000455         2204 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_366013, whole genome shotgun sequence.
ACCESSION   DATCCH010000455 DATCCH010000000
VERSION     DATCCH010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366013"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="VIJ44_08780"
     CDS             <1..558
                     /locus_tag="VIJ44_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEY5166032.1"
                     /translation="GRLGYPRRARRLWEAASRIASDGWPEDLTELPGVGRYTAGALRA
                     QTDDADVAAVDVNIRRVVERVVGRRLSVRDTETEMVRVGRPLRGRDRLLALMDVGALL
                     CRPADPRCSSCPLRRGCRTRGALPPVQRARPAPFDGSFRQRRGSVLARLRAGPTPLAL
                     LDHEALESLRRDGLAVADDTTARLP"
     gene            complement(451..1158)
                     /locus_tag="VIJ44_08785"
     CDS             complement(451..1158)
                     /locus_tag="VIJ44_08785"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011431437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEY5166033.1"
                     /translation="MGRRVVVIDNYDSFVYNLVQYLGELGAEPVVYRHDDLDLAGLQE
                     LEADALLVSPGPGRPEDAGLSNDAIVDCAARGVPVLGVCLGHQCIGHVFGGEIVRASQ
                     VMHGKTSVISHDGAGVFAGLPNPLRATRYHSLVVDRGSVPAELIVSAESEDGSVMGLR
                     HRSLPVEGVQFHPESILTESGHALLRNFLSPRRGRRTRLDLGQPRRGVVGDGEPIAAK
                     RFQCFMVEQREWRGTGA"
     gene            1209..1469
                     /locus_tag="VIJ44_08790"
     CDS             1209..1469
                     /locus_tag="VIJ44_08790"
                     /inference="COORDINATES: protein motif:HMM:NF018487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CrgA"
                     /protein_id="HEY5166034.1"
                     /translation="MPKSKPRKGPPAAARTRSRYTPPKPKTAKRSPLWVPAAMFATLG
                     AGVIVILGNYLELLPGGTARNGYLFLGLGLLIGGFVMSTQLR"
     gene            complement(1550..1840)
                     /locus_tag="VIJ44_08795"
     CDS             complement(1550..1840)
                     /locus_tag="VIJ44_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5166035.1"
                     /translation="MVGEALFAVSTVGWVVRVVIALVAMLAIYLVGAGTLRKFKIAPD
                     SEPDPSQVVPVDARFACIVCGAEVTMTAAQDGAAIEAPRHCREDMVPVGPVG"
     gene            complement(1857..>2204)
                     /locus_tag="VIJ44_08800"
     CDS             complement(1857..>2204)
                     /locus_tag="VIJ44_08800"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013422445.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEY5166036.1"
                     /translation="SVRGADPIWMLTAPIPATAHALARSGMSLDDIDLVEINEAFAPV
                     VLAWQQEHHLDMSRVNVNGGAIALGHPLGCSGARLMATLLNELERSGGRFGLQTMCEG
                     GGMANATILERLG"
BASE COUNT          355 a          805 c          737 g          307 t
ORIGIN      
        1 ggccgcctcg gctacccccg ccgagcccgg cgcctctggg aggccgcgtc ccgcatcgcg
       61 agtgacgggt ggcccgagga tctcaccgag ctgcccgggg tcgggcgcta caccgcgggg
      121 gcgctccgcg cccaaaccga cgacgccgac gtcgcggccg tcgacgtcaa cattcgacgc
      181 gtcgttgagc gggtcgtcgg acgacggctc agtgtccgcg acaccgagac ggagatggtc
      241 cgcgtcggtc ggccgttgcg aggccgcgac cggctcctgg ccctgatgga cgtcggggcg
      301 ctgctgtgcc gccccgccga cccgcgttgc agcagctgtc cgctccggcg ggggtgccgc
      361 acccgggggg cgcttccccc ggtgcaacga gcccgaccag ccccgttcga cggctcgttc
      421 cgccagcgcc gcgggtccgt cctggcccgg ttacgcgccg gtcccacgcc actcgcgctg
      481 ctcgaccatg aagcactgga atcgcttcgc cgcgatgggc tcgccgtcgc cgacgacacc
      541 acggcgcggc tgccctaggt cgagacgggt tcgacgaccc cggcgggggg acaagaagtt
      601 cctgagcagc gcgtggcccg actcggtgag aatcgactcg gggtggaact ggaccccttc
      661 gaccgggagg cttcggtggc gcagccccat gacggagccg tcctcgctct ccgcgctcac
      721 gatcagctcc gcgggaacgg acccgcggtc cacgaccagc gagtgatacc gggtcgcccg
      781 caatgggttc gggagaccgg cgaacacccc cgccccgtcg tggctgatca ccgaggtctt
      841 gccgtgcatc acctgcgaag cgcgcacgat ctcgccgcca aagacgtggc cgatgcactg
      901 atgcccgagg cacacgccga ggaccggcac cccccgcgcc gcgcagtcga cgatggcgtc
      961 attcgagaga ccggcgtcct cgggacgtcc cggccccggc gagacgagca gagcgtccgc
     1021 ctcgagctct tgcaggcccg ccaagtcgag atcgtcgtgg cgatacacga ccggctccgc
     1081 gccgagctct ccgagatatt gcacgaggtt gtacacgaac gagtcgtagt tatcgatcac
     1141 gaccacgcgc cggcccacca gcggcactgt agaggagtga gtgaagccgg gaagtagggt
     1201 tcgcgctcgt gccgaagagc aagcctcgca aaggaccgcc cgcggccgct cggacgcggt
     1261 cgcgatacac gcctccgaag cccaagaccg cgaagcgaag cccactgtgg gtcccggccg
     1321 cgatgttcgc caccctcggg gcgggcgtca tcgtcatcct cggcaactac ctcgaactgc
     1381 tccccggagg cacagcccgc aacgggtacc tgttcctcgg gcttggcctc ctcatcggcg
     1441 ggttcgtcat gtccacccag ttgcgctgac ccgaatccgg agaaatgaca caactgtcat
     1501 tcctccccag gttgctccac agtctgtgga gaaccccgga gaacccgggt cagccgacgg
     1561 gtccgaccgg caccatgtcc tcccgacagt ggcgaggcgc ctcgatggcg gcgccgtctt
     1621 gggctgcggt catggtgacc tcggccccgc agacgatgca ggcgaaccgg gcgtcgaccg
     1681 ggaccacctg ggagggatct ggctccgagt cgggggcgat cttgaacttc cgcaacgtcc
     1741 ccgccccgac gaggtagatg gcgagcatcg ccaccagggc gatgaccacc cggacgaccc
     1801 aacccaccgt cgaaaccgcg aagagcgcct cgccgaccac caagagtcag cgactcctag
     1861 ccgagccgct cgaggatcgt ggcgttggcc atccccccgc cttcacacat ggtctgcaga
     1921 ccgaagcgcc ctcccgagcg ctcgagctcg ttcaggaggg tcgccatcaa gcgggcgccg
     1981 gagcagccga ggggatgccc gagcgcgatc gcgcctccgt tgacgttgac gcgactcatg
     2041 tcgaggtgat gctcctgctg ccacgccagc accacgggcg cgaacgcctc gttgatctcg
     2101 accaggtcga tgtcgtcgag gctcatcccg ctgcgggcga gcgcatgcgc ggtcgcgggg
     2161 atcggagcgg tgagcatcca gatcgggtcc gcgccccgca ccga
//
LOCUS       DATCCH010000456         3959 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_407251, whole genome shotgun sequence.
ACCESSION   DATCCH010000456 DATCCH010000000
VERSION     DATCCH010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407251"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..1259)
                     /locus_tag="VIJ44_08805"
     CDS             complement(75..1259)
                     /locus_tag="VIJ44_08805"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009742234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEY5166037.1"
                     /translation="MPGSVIVSFARTPIGKLSGALGSLTAMELGGRAIQGALERGGVG
                     PEQVDYVVMGHVLQGGQGQITARQAAARAGIPMTVPALTINKVCLSGLNAVYLADQMI
                     QAGDAEIVVAGGMESMTNAPYLLPKARAGYRMGNGELLDSLIQDGLWCAFDAVHMGSG
                     TERYTGRFGGLTREAQDEFAAKSHERAAVAQKEGRFADEITPVQIPQRKGDPILVEED
                     EGVRPETTAESLGRLKPAFDADGSITAGNASQISDGAAAIIVTSRSKAEELSLTPVAE
                     LVSFGMVAGPDTSLLRQPSRAIKRALEPIDKTVSDLDLLELNEAFAAVGLASMKDLGI
                     SDDIVNVNGGAIALGHPIGMSGARVLLTLINELRRRGGGLGAAALCGGGGQGDAAIVR
                     VA"
     gene            1491..2834
                     /gene="ccrA"
                     /locus_tag="VIJ44_08810"
     CDS             1491..2834
                     /gene="ccrA"
                     /locus_tag="VIJ44_08810"
                     /EC_number="1.3.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043880 - crotonyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonyl-CoA carboxylase/reductase"
                     /protein_id="HEY5166038.1"
                     /translation="MSEIDAIRDAILARAPGDEVGALPLPGTFRAAVVRADEQDMFAG
                     IPSEAKDPRKSVHIDDVPLPELAPDEAYVAVMASSINFNTVWTAIFEPLPTFQFLARL
                     GKESVWGARHDQPFHVLGSDAAGVVVRVGSAVRKWRPGDAVTVHCNYVDDQDPSAHDD
                     SMLASNQRIWGFESNYGGLADLTVVKANQLMPKPAHLTWEEAAVNALCNSTSYRMIVS
                     PHGAQMIQGQTVLVWGASGGIGAYACQYVLNGGGTPVGVVSSPDKVALLHELGVEHVI
                     DRTAEGYRFWSDEHTQDPGEWRRLGKKIRELAGTDPDIVFEHPGRQTMGASVFVAKRG
                     GLVVTCAATSGYTIEYDNRYLWMNLKTLKGCHFANYRESWAANQLVCEGKIQPTLSQV
                     FTLEETGTAAYEVHHNRHEGKLGILCLAPEEGLGVTNPERRAELLPQITLFRRHA"
     gene            2886..3290
                     /gene="mce"
                     /locus_tag="VIJ44_08815"
     CDS             2886..3290
                     /gene="mce"
                     /locus_tag="VIJ44_08815"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HEY5166039.1"
                     /translation="MTVLTEIDHIAIAVHDLEAAIAYYRDTFGCEVAHREVVEADGVE
                     EALLKVADSYIQLLTPTRDDSPVAKSLATRGEGLHHVGYRVQDCAAALARVRAEGHRV
                     IDEEPRPGSRGTTVAFLHPKSAFGTLIELVQE"
     gene            3403..>3959
                     /locus_tag="VIJ44_08820"
     CDS             3403..>3959
                     /locus_tag="VIJ44_08820"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY5166040.1"
                     /translation="MTFAAACVVALVVLYGLAVHTSIGQHLDNDALLGRSQNLRVQQA
                     TNHLLHTIDVFSLALIGGAIVLGAIARARLRLALGAAALILGAIALTEGLKHALPRPA
                     LLNPDPLPLPSFPSGHTAVALSLALALLLVVPVRWRGPTAVAGALYGAAVGAATLTAA
                     WHRPSDVIGAFLVTTGWAAAVGAVLV"
BASE COUNT          616 a         1467 c         1276 g          600 t
ORIGIN      
        1 gcgggggctc gtcaccgggc cagtgtcggt tgtgccgtgt ttgggccggg ccgggaccga
       61 tgggctcggt cggttcaggc cacccgcacg atcgcggcgt cgccttggcc gccgccaccg
      121 cagagcgccg cggcgccgag accgccgcca cgccggcgga gctcgttgat gagggtcaac
      181 agcacccgcg ctccggacat cccgatcgga tggccgagcg cgatcgcgcc gccgttcacg
      241 ttcacgatgt cgtcgctgat cccgagatcc ttcatcgagg ccagaccgac cgcggcgaac
      301 gcctcgttca gctccaagag gtcgaggtcc gacaccgtct tgtcgatcgg ctccaacgcc
      361 cgcttgatgg cgcgtgatgg ctggcgcagc agggaggtgt ccgggcctgc caccatgccg
      421 aagctgacga gctcggcgac cggggtcaag ctgagctcct cggccttcga gcggctggtg
      481 acgatgatcg ccgccgcgcc gtcggagatc tgggaggcgt taccggcggt gatcgagccg
      541 tcggcgtcga aggctggctt gagccggccc agcgattcgg cggtggtctc gggccggacg
      601 ccttcgtcct cttccacgag gatgggatcc cccttgcgtt gggggatctg gaccggcgtg
      661 atctcgtcgg cgaagcggcc ttccttctgg gcgaccgcgg cccgctcgtg gctcttcgcc
      721 gcgaactcgt cttgggcctc ccgggtcagc ccgccgaacc ggccggtgta ccgctcggtg
      781 cccgaaccca tgtgcacggc gtcgaacgcg caccaaagac cgtcctggat cagggagtcg
      841 agcagctcgc cgttacccat ccggtagccg gcgcgggcct tgggcagcag atacggcgcg
      901 ttcgtcatcg attccatgcc accggcgacg acgatctcgg cgtcgccggc ctggatcatc
      961 tggtcggcca ggtacaccgc gttcagtccc gacaggcaca ccttgttgat cgtcaacgcc
     1021 ggaaccgtca tcgggatgcc agcgcgcgcg gcggcctgcc gggcggtgat ctgaccttgt
     1081 ccgccttgga ggacatggcc catcacgacg tagtcgacct gctctggtcc gacgccgccg
     1141 cgctcaagcg cgccttggat ggcgcgtccc ccaagctcca tggccgtcaa cgacccgagc
     1201 gcacccgaca acttcccgat gggcgtgcgg gcaaacgaga cgatcacgga gccgggcatc
     1261 ggacctcctg tttctcacaa gcggcgagca agtagccatc gatggtaccc ctggctcgcc
     1321 gggcgcgccc gagtcggaca cgcccgcgta tggttctgac ccatgatcac gattcccgaa
     1381 ccgcgaggag cgcgaacgcc gcgccccgac cgtccccgcc gccggtgtcc cgaccacgaa
     1441 gtgcggcccg tgctcggcga ggccggtccg agacgcccgg cacgacaccg atgagcgaga
     1501 tcgacgccat tcgcgacgcg atcctcgcca gggcgcccgg cgacgaggtc ggcgccctcc
     1561 cgttacctgg caccttccga gccgcggtcg tgcgcgccga cgagcaagac atgttcgccg
     1621 gcatcccctc cgaagccaaa gacccgcgca agtcggttca catcgacgac gtgccgctcc
     1681 ccgagcttgc ccccgacgag gcgtacgtcg ccgtcatggc atcgtcgatc aacttcaaca
     1741 cggtctggac cgcgatcttc gaaccgctcc ccacgttcca gttcctcgct cggctcggca
     1801 aggagagcgt gtggggagcg cggcacgatc agccgttcca tgtgctcggg agcgacgccg
     1861 cgggtgtcgt cgtgcgtgtc ggctccgcgg tgcggaaatg gcgaccgggc gacgcggtga
     1921 ccgtgcactg caactacgtc gacgaccagg acccgtcggc ccacgacgac tcgatgcttg
     1981 cctccaacca gcggatctgg ggtttcgagt cgaactacgg cggcctcgcc gacctcacag
     2041 tggtcaaggc caatcagctc atgcccaagc cagcccacct cacctgggag gaggccgcgg
     2101 tcaacgccct gtgcaactcg acgagctacc gaatgatcgt cagcccccac ggcgcccaga
     2161 tgatccaagg ccagaccgtc ctggtgtggg gggcgagcgg agggatcggc gcctacgcct
     2221 gccagtacgt cctcaacggg ggagggaccc cggtcggggt ggtgtcctcg cccgacaagg
     2281 tggcgctcct ccacgagctc ggggtcgagc acgtgatcga ccgcacggcc gagggctacc
     2341 ggttctggag cgacgagcac acccaagacc ctggtgagtg gcgccgactc gggaagaaga
     2401 tccgtgagct ggccggcacc gaccccgaca tcgtcttcga gcacccggga cgacagacga
     2461 tgggcgcctc ggtgttcgtc gcgaaacggg gtggcctcgt cgtcacctgc gccgcgacga
     2521 gtggctacac gatcgagtac gacaaccgat acctgtggat gaacctcaag acgttgaaag
     2581 gctgtcactt cgccaactac cgcgaatcgt gggcggcgaa ccagctcgtc tgcgaaggca
     2641 agatccaacc gaccctgtcc caggtcttca ccctcgagga gaccggcacc gccgcgtacg
     2701 aggtccacca caaccgtcac gaaggcaagc tcggcatctt gtgtctcgcc cccgaggaag
     2761 ggctcggggt cacgaacccg gaacggcgcg ccgagctgct cccccagatc accctctttc
     2821 gacgccacgc ctgacacgca ccctcgaacg aatcggattc ggtcggcggc ggatagcgtg
     2881 aacgcatgac cgtgctcacc gagatcgacc acatcgcgat cgcggtccat gacctcgagg
     2941 ctgcgatcgc ctactaccgc gacaccttcg gctgtgaggt cgcgcaccgc gaagtcgtgg
     3001 aagccgacgg cgtcgaggaa gcgctactca aggtggccga ttcgtacatc cagctgctca
     3061 ccccgacgcg agacgactca ccggtcgcga agtcgctggc gacccgaggg gaaggcctcc
     3121 accacgtcgg ctaccgggtc caggactgcg ccgcggcgct ggcccgggtg cgagccgagg
     3181 ggcaccgcgt gatcgacgag gaaccccgcc cggggagccg aggcacgacc gtcgccttct
     3241 tgcaccccaa gtcggcgttc gggacgctca tcgagctcgt ccaggagtaa cggtccggca
     3301 tccgccggct cctccgaccg tcgcccccga ggggtccgcc gccagactgg acccatcgac
     3361 gctgcgcccg acgacctggc ccgccggcgc gccaccgcgc tgttgacgtt cgccgccgcg
     3421 tgcgtcgtcg ccctggtcgt cctgtacggc ctcgccgtcc acaccagcat cgggcagcac
     3481 ctcgacaacg acgccctcct cggccggtcc cagaacctcc gggtccagca ggccaccaac
     3541 catctcctcc acaccatcga tgtgttctca ctcgcgttga tcggcggggc gatcgtgctc
     3601 ggcgccatcg ctcgcgcccg gctccgcctc gcgctcggcg cggccgcgct gatcctcggc
     3661 gccatcgccc tcaccgaggg cctcaagcac gcgctccccc gacccgcgct gctgaacccg
     3721 gacccactcc cgcttccgag cttcccgagt ggccacaccg ccgtcgccct ctccctcgcc
     3781 ttggccctgc tcctcgtggt ccccgtccga tggcgcggcc ccaccgccgt cgccggcgcc
     3841 ctctacggcg ccgccgtcgg tgccgccacg ctcactgcgg cctggcatcg gcccagcgac
     3901 gtcatcggcg cgttcttggt caccaccggc tgggccgcag ccgtcggcgc ggtcctcgt
//
LOCUS       DATCCH010000457         3348 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_407509, whole genome shotgun sequence.
ACCESSION   DATCCH010000457 DATCCH010000000
VERSION     DATCCH010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407509"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1290)
                     /locus_tag="VIJ44_08825"
     CDS             complement(<1..1290)
                     /locus_tag="VIJ44_08825"
                     /inference="COORDINATES: protein motif:HMM:NF019974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate- and nitrite sensing domain-containing
                     protein"
                     /protein_id="HEY5166041.1"
                     /translation="MSVPLFALVGLAGVTIHSRFDALSNQQQYGHLLPAFDALTHLGR
                     AAADEGVAAQWFIQVRQGDPPASKALIFDTRARTDDATHALWATYPALVGRVTSQTLT
                     AVRAVRDRLQALIDVARVQVDAHINPGPAFSDVAASAVAASAAVARDVPDRDLSTGLL
                     GVVNLRRGQLAAATEGSIVTNVLLDGRGDVTAWDAAIHDQDNATNAFLETATQKESGA
                     YTRAVSPPAVTPLRTNGAALPTAFPATNLSLYDYQHLYLAKQAQTDRGINAVQTVIGQ
                     SAITKEADARHQLVLASGGTALLIGIVLLLAWALVQSVNKPLRALTRAARNMSERRLP
                     QLVDTLRAGGELSPGQLEELTPIRVQADDEIGALARAFSTIQHVTVAVAEEQAALLRK
                     GIGDLYVNLARRNQSLLDRQLALLDDLEGRAAEPEELA"
     gene            complement(1446..>3348)
                     /locus_tag="VIJ44_08830"
     CDS             complement(1446..>3348)
                     /locus_tag="VIJ44_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEY5166042.1"
                     /translation="AIAGPLRAVAGTVGAAVVLAGLGVLGALLAPAVPLRRVATSIAR
                     GARWFARQWRLADRPPSRRPPPSPPTDLDQPTVEPRVDEPPVDDYADPTAASAVYDVE
                     RDRPPTDPPSEDATQPDARPVPVVEEAGGQMALDVGRRGHPGAWKLPPPTALTRSSAA
                     KADRRLVEEGGRVLEAALHQHGVDAHLVGMTVGPTVTRYELELAPGVKVNRITSLAHD
                     VAYAMATPDVRILAPIPGRSAIGVEVPNKQRQLVTLGDILASPEAKQATSPLEVGLGR
                     DIAGRPVMLDLATLPHVLIAGATGAGKSSCINSLVTSLLMRNTPDQVRLILVDPKRVE
                     LGAYNDLPHLLTAVVTNPKKAANALDWAVREMDLRYEMLAEVGVRDVAGFNAAYDRGD
                     LPTPGDPDPVTGRGYERLPYIVVVVDELNDLMMVAARDVEASICRIAQMARAVGIHLV
                     IATQRPSVDVITGVIKANVPSRMAFSVTSLADSRVILDQSGAEKLVGQGDMLLVTASE
                     TRPRRIQGAWVDEEGVHNVAAHWRRQVVEPHYVDGIAGDGSPGTSRPGGNASDDDDLL
                     EEAMELVVRSGLGSTSMLQRKLRIGFARAGRIMDLLERRGVVGPSEGSKARTVLMTVD
                     ELEARPVST"
BASE COUNT          436 a          949 c         1445 g          518 t
ORIGIN      
        1 ggcgagctcc tcgggttcgg cggcacggcc ttcgaggtcg tcgaggaggg cgagctggcg
       61 gtcgaggagg ctctggttgc ggcgggcgag gttgacgtag aggtcgccga tgcctttgcg
      121 gagcagcgcg gcttgctctt cggcgacggc gacggtgacg tgctggatgg tgctgaacgc
      181 gcgcgcgagg gcgccgatct cgtcgtcggc ttggactcgg atcggggtga gctcttcgag
      241 ctggccgggt gagagctccc cgccggcgcg gagggtatcg acgagctgcg ggaggcgacg
      301 ttcggacatg ttgcgagcgg cgcgggtgag ggcgcgcagc ggcttgttga cggactggac
      361 gagcgcccag gcgaggagga gcacgatgcc gatgagcagc gcggttccgc cggaggccag
      421 gacaagctgg tggcgggcgt cggcttcctt ggtgatggca ctctgaccga tgacggtctg
      481 caccgcgttg atgccccgat cggtctgggc ttgtttggcg agatagaggt gctggtagtc
      541 gtagaggctg aggttcgtcg ccgggaacgc ggtcgggagg gcggcgccgt tggtgcgcag
      601 cggggtgacg gccggggggc tcacagcgcg ggtgtaggcg ccggattctt tttgggtggc
      661 ggtctcgagg aacgcgttgg tggcgttgtc ctggtcgtga atggcggcgt cccaggcggt
      721 gacgtcaccg cggccgtcga ggagcacgtt ggtgacgatc gagccttcgg tggcggcggc
      781 gagctggccc cggcgaaggt tgacgacgcc gaggagcccg gtggagaggt cgcggtcggg
      841 gacgtcgcgg gcgacagccg cggaggccgc caccgcgctg gccgcgacgt cgctgaacgc
      901 cggaccgggg ttgatgtggg cgtcgacttg gacccgggcg acgtcgatga gcgcctggag
      961 gcgatccctg acggcccgca ccgcggtgag ggtctggctg gtgacccggc cgacgagcgc
     1021 ggggtaggta gcccagaggg cgtgggtggc gtcgtcggtg cgggcccggg tgtcgaagat
     1081 gagggccttc gaggcggggg gatcgccctg gcggacctgg atgaaccatt gggcggcgac
     1141 gccttcgtcc gcggcggcgc gtccgaggtg ggtgagggcg tcgaaggcgg ggaggaggtg
     1201 cccgtattgc tgctggttgg acagcgcgtc gaagcggctg tggatggtga ccccggcgag
     1261 gccgacgagc gcgaagagag ggacgctcac caccaagatg agcttcgacc ggaatggaag
     1321 ctttcccagc atgcagtccc cggggcgcat gcccgccggg atggtttacg gcacggggtt
     1381 gagcaccctt gaggcccgac cggggaacgc gccgaggggt ggcgggaggc ccgggtgggg
     1441 tggggtcagg tggagacggg gcgggcttcg agctcgtcga cggtcatgag gaccgtgcgg
     1501 gccttcgagc cttcggaggg gccgaccacg ccgcggcgtt cgaggagatc catgatgcgg
     1561 ccggctcggg cgaacccgat tcgcagcttg cgttgcagca tcgacgtgga gcccagcccg
     1621 gagcggacca cgagctccat ggcctcttcg aggaggtcgt cgtcgtcgct ggcgttaccg
     1681 ccggggcggc tggtgccggg gctgccgtcg ccggcgatgc cgtcgacgta gtggggctcg
     1741 acaacttggc gtcgccagtg ggcggcgacg ttgtggacgc cttcctcgtc gacccaggcg
     1801 ccctggatgc gtcgggggcg ggtctcgctg gcggtgacga gcagcatgtc gccttggccg
     1861 acgagcttct cggcgccgga ctggtcgagg atgacgcgcg agtcggcgag gctggtgacc
     1921 gagaacgcca tgcggctcgg gacgttggcc ttgatgacgc cggtgatgac gtcgaccgag
     1981 ggacgctggg tggcgatcac caggtggatg ccgacggcgc gggccatctg ggcgatgcga
     2041 cagatgctcg cctcgacgtc gcgggcggcg accatcatga ggtcgttgag ctcgtcgacg
     2101 acgacgacga tgtacgggag gcgttcgtag cctcggccgg tgaccgggtc ggggtcgccg
     2161 ggggttggga ggtcgccgcg gtcgtacgcg gcgttgaacc cggcgacgtc gcggacacca
     2221 acctcggcga gcatctcgta gcgcaggtcc atctctcgga ccgcccaatc gagcgcgttg
     2281 gcggccttct tggggttggt gaccaccgcg gtgaggaggt gcgggaggtc gttgtaggcg
     2341 ccgagctcga cgcgtttggg gtcgacgagg atgaggcgga cctggtcggg cgtgttgcgc
     2401 atcaagaggg aggtgaccag cgagttgatg cagctcgact tgccggctcc ggtggcgccg
     2461 gcgatgagga cgtgcgggag cgtcgcgagg tcgagcatga cgggacgacc ggcgatgtcg
     2521 cgtccgaggc cgacctcgag cgggctggtg gcttgcttcg cttcggggga ggcgaggatg
     2581 tcgccgaggg tgacgagctg gcgttgtttg ttgggaactt cgacaccgat ggcgctgcga
     2641 ccggggatgg gcgcgaggat gcgcacgtcc ggtgtcgcca tggcgtaggc gacgtcgtgg
     2701 gcgaggctgg tgatgcggtt gaccttgacg ccgggggcga gttcgagttc gtagcgggtc
     2761 acggtcggac cgacggtcat gccgacgagg tgcgcgtcga ccccgtgttg gtgcagcgcg
     2821 gcttcgagga cccggccgcc ttcctccacg aggcgccggt cggctttcgc ggcgctgctg
     2881 cgggtgaggg cggtgggcgg cgggagcttc caggctccgg ggtggccgcg acggccgaca
     2941 tcgagggcca tctggccgcc ggcctcctcg acgacgggga cgggacgggc atcgggttgg
     3001 gtggcgtcct cggagggagg atcggtcggc gggcggtcgc gttcgacgtc gtagaccgca
     3061 ctggcggcgg tggggtcggc gtagtcgtcg accgggggct cgtcgacgcg gggctcgacc
     3121 gtcggctggt cgagatcggt cggcggggag ggcggcggcc gacggctggg gggacggtcg
     3181 gcgaggcgcc attggcgggc gaaccaacgg gcgccgcggg cgatcgaggt cgcgactcgc
     3241 cgcagcggga cggcgggcgc gaggagcgcg ccgaggacgc cgagacccgc gaggaccacg
     3301 gcggcgccga cggtgccggc cacggcccgc agcggaccgg cgatcgcg
//
LOCUS       DATCCH010000458         2160 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_407670, whole genome shotgun sequence.
ACCESSION   DATCCH010000458 DATCCH010000000
VERSION     DATCCH010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407670"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..1258)
                     /locus_tag="VIJ44_08835"
     CDS             complement(101..1258)
                     /locus_tag="VIJ44_08835"
                     /inference="COORDINATES: protein motif:HMM:NF012427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY5166043.1"
                     /translation="MRILFTSSPGWGHVHPLVPLAQAFLERGDEVLWGTGAESARRLA
                     AEGIPAAGAGLEAQVAMAELFRRFPEVHDVAPASRSDFMFPRLFGTIKAPAMFADLAP
                     VAREWSPDLVVSDTAEFAGPLIAATLGIPAVSHAFGGLLPEPRVSATAEAVEALWKEA
                     GLDPRPYGGMYDHLYLDIFPPSLQPAERPHVSATQLLQPGAFASAGDEVTPDWVTSST
                     GTPLVYVTLGTVFSNDAALAAVVEGVRALDVRVVVTVGPHGDPAALGVQPSNVHVARY
                     IPQEQLLPACAAVVSHGGSGTFLAALAAGLPQVCVPQAADQFFNAAAAVRSGIGFAFL
                     PDEVDVTAIRGAVQQLLTESAYSAAARRISTEIAAMPDARTVAGALAQLFG"
     gene            complement(1774..>2160)
                     /locus_tag="VIJ44_08840"
     CDS             complement(1774..>2160)
                     /locus_tag="VIJ44_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003884464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5166044.1"
                     /translation="DQDEALAFWTDKVGFEIREDVTVPEMGNFRWLSVGPRDQDQIAL
                     VLMAIPGPPVLDEATRAQVAELTAKGFASTLFLTTDDAMKAYEELSARGVEFTEKPTV
                     QPYGIDCGFRDPSGNSIRLAQRTPMS"
BASE COUNT          334 a          702 c          775 g          349 t
ORIGIN      
        1 cgaggtattc gtgtcggcgg gcgcgttctc catgggcttc gattggtgct gaggtcgttc
       61 tgaccacgtt tgccgaggtt cgaagcgagg gccgagtcac tcagccgaag agttgtgcga
      121 gcgctcccgc gacggtgcgc gcgtcgggca tcgcggcgat ttcggtgctg atgcggcgcg
      181 ccgccgccga gtaggccgac tcggtgagga gttgctgcac tgcgccgcgg attgccgtca
      241 cgtcgacttc gtcgggcagg aaggcgaagc cgattccgct tcggaccgcg gctgccgcgt
      301 tgaagaactg atcggcggct tgaggcacgc acacctgagg gagtcccgcg gcaagtgctg
      361 cgaggaaggt gccggatccg ccgtgcgaca cgacggcggc acaggccggt aggagttgct
      421 cttgcgggat gtaacgcgcc acgtggacat tgctcggctg gaccccgagt gcggccgggt
      481 cgccgtgcgg accgacggtg acgacgactc gtacgtcgag cgcgcgaaca ccctcgacga
      541 ccgccgcgag cgcggcatcg ttgctgaaca cggtgccgag ggtcacgtag accagcggcg
      601 tccccgtgga cgacgtgacc caatccggcg tgacttcgtc gcccgcggat gcgaatgcgc
      661 cgggctgcaa tagttgcgtc gccgagacgt gcgggcgctc ggcgggctgc aggctcggcg
      721 ggaagatgtc gaggtacaga tggtcgtaca tgcctccgta gggccgcggg tcgagccccg
      781 cttccttcca caacgcctcg acggcttcgg ccgtcgcgga cactcgcggt tcagggagca
      841 ggccgccgaa cgcgtgactg accgccggga tccccagcgt tgccgcgatg agcgggccgg
      901 cgaactcggc ggtgtcgctg accacgaggt cgggcgacca ctcgcgggcg accggagcga
      961 ggtcggcgaa catggctggt gctttgatcg tcccgaacag ccgggggaac atgaagtcgg
     1021 atcgtgaggc cggcgcgacg tcgtggacct ccgggaaccg acggaagagt tccgccatag
     1081 ccacctgagc ttcgagaccg gcaccggccg ccgggatgcc ctcagccgcg aggcgtcgtg
     1141 cgctctcggc gccggttccc cacaggacct cgtcgccacg ttcgaggaag gcctgggcga
     1201 gcgggaccag ggggtggaca tggccccaac cgggcgatga agtgaacaag atccgcatcg
     1261 gagacctcct gcgccgcgag cggctgctct gtccggagga ccataacccg aacatcacta
     1321 gaagtctagt gacttgattt ctagctaatt gtcttatcgt ggcggtactc gccgccgccc
     1381 tcgacgctga ccggcgcgtc gcgtggatag ggccccgggg tcgccgcccc ggggacgcgc
     1441 gacctcgggc cacccagccc cggcgtctgg gccgtgcatc tggcagcggg aatcaagacc
     1501 acggccaacg ctgtcgctcg cacgtgagtg agaggcagca acggtctggt tggttgtcgc
     1561 gacttgtctg cgcgcaaagg tcgtggccct cgtcacccgg gcacctggcg ggcgtgcgac
     1621 ggaccgccta acttggcgct cagcgtgcgg gtatctgagc gtggcgctca gcgtcagctc
     1681 cggtgctgca cactgccgcc cgacttgtcc gtgtgagaac gggccagagc ctgggctcac
     1741 acggacaggt cggcagagct cgtcggcaac gggtcagctc atcggggtcc gctgggcgag
     1801 ccggatgctg tttcccgacg ggtcgcggaa gccgcagtcg atgccgtagg gctggaccgt
     1861 gggcttctcg gtgaactcca cgccacgtgc cgacagctcc tcgtacgcct tcatggcatc
     1921 gtccgtcgtg aggaagaggg tgctcgcgaa gcccttggcc gtgagctccg cgacctgggc
     1981 ccgcgtcgcc tcgtccagga ccggcggccc cgggatagcc atgagcacga gggcgatctg
     2041 gtcctggtct ctcggtccga cggacagcca gcggaagttc cccatctcgg gcaccgtcac
     2101 gtcctcccga atctcgaagc cgaccttgtc ggtccagaag gcgagcgcct cgtcctggtc
//
LOCUS       DATCCH010000459         4999 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_75826, whole genome shotgun sequence.
ACCESSION   DATCCH010000459 DATCCH010000000
VERSION     DATCCH010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4999
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75826"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1057
                     /gene="cofH"
                     /locus_tag="VIJ44_08845"
     CDS             <1..1057
                     /gene="cofH"
                     /locus_tag="VIJ44_08845"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HEY5166045.1"
                     /translation="DQLRRDTVGETVTFVRNRNINYTNICTFKCRFCAFSKGPLSLNL
                     RGDPYLLDVGEIQRRVVEAVEHGATEVCLQGGIHPDFDGAYYLGVTRAVKDVAPDIHV
                     HGFTALEVTEGARRLGIPLRDYLRELKAAGLSTLPGTAAEILDDEVRAIICPDKVTTD
                     EWLDAHRDAHSVGLRSNVTIMFGSVEAPVHVARHLTRTRSLQKETGGFTEFVPLPFVH
                     MATPISLQGRARRGPTFREVLLLHAVGRIAYRGWIDNIQISWVKVGIRGAQQALQAGC
                     NDLGGTLMDENISRAAGASHGQELDDDGFAAIVGPLGRPLEQRTTLYGRTRTVGRALR
                     PPVPEPTPVALTARAAL"
     gene            1060..2064
                     /locus_tag="VIJ44_08850"
     CDS             1060..2064
                     /locus_tag="VIJ44_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HEY5166046.1"
                     /translation="MPASDGSTVDVVGVGNALVDVLSLATDEFLDDHGLTKGAMALID
                     ADQAERLYAAMGPGTEMSGGSAANTMVGVSSLGGTSAFVGRIRDDTLGRVFAHDIQAS
                     GVRYDTAAAPAGHPTGRCLILVTPDAERTLNTFLGAASELAVADVSETLVRGAPVTYL
                     EGYLFDQPDAKEAFRHAARLAHAAGRRVALSLSDTFCVERHHADFLALVEHGVDILFG
                     NESEIRALYRVNRLDDAFELVARHCELAALTRGARGSVVVTPEGIHEVEVHPVAGPVI
                     DTTGAGDQYAAGFLFAFTHGSDPVTCGRVGALAASEVISHLGARPERPLAELAAPLLP
                     "
     gene            2061..3104
                     /locus_tag="VIJ44_08855"
     CDS             2061..3104
                     /locus_tag="VIJ44_08855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEY5166047.1"
                     /translation="MKLPRYRTGDPELDDAVAALVERVADDDADLVFELVVSAIRLAR
                     DHADRGDLKMANAALKEMRYSFHVFEPYRAARKVAIFGSARTQPDDPLYHQARRFAEA
                     VAARDWMVITGAGPGIMEAGIEGAGPDHAFGVSIRLPFEAASTQFIADDPKLINFRYF
                     FTRKLAFVKEADAFVLLPGGYGTMDETFELLTLMQTGKAQPGPVVLLDTPGGTYWEGW
                     LEFVRGELLTNRYVSGPDLELLFVTDDVDAAVGEVTDFFANYHSQRFVEGRLVLRVRR
                     APPPATLDALSEEFRDIVRRGAIERVDPSPAEIADDDALDLERVAFWFDRHGWARLRE
                     LIDRLNGHEPHTN"
     gene            complement(3101..3298)
                     /locus_tag="VIJ44_08860"
     CDS             complement(3101..3298)
                     /locus_tag="VIJ44_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5166048.1"
                     /translation="MSEPGLGDVVETLRGIEERLRDAAYDRLRAAADGDEDAVKEERR
                     LQQARRAVERALRALGAEPDG"
     gene            complement(3392..4426)
                     /locus_tag="VIJ44_08865"
     CDS             complement(3392..4426)
                     /locus_tag="VIJ44_08865"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY5166049.1"
                     /translation="MDLTGQRSEADVQAVVADAETVVAVGGGTHREVGNPVIAATPVD
                     APRGIVSYDPAELTVTAWAGTTVGELDATLAADHQECVLDGRDPTGTIGGLLAVGLSG
                     HRRLRYGPLRDRVLEVRFVTAAGRVVRGGGPTVKNVTGYDMPRLLVGSFGTLGILTRV
                     ILRCQPRPQCIRWFGTDLEPTAVRARCFQPSAIFWHPDTGSQVLLEGHPDDVDAQARA
                     ASLDPLDEAPTFPDGPHQGRISVAPDRISQLGHELESIGEVTWTAEWGVGTVHVAALS
                     EAALSEARAAAHVAQGWLLRTAGAAGLDGFGIEFPNGALMARIKMAFDPEAKLAPGRL
                     PIPQPEREAS"
     gene            complement(4417..>4999)
                     /locus_tag="VIJ44_08870"
     CDS             complement(4417..>4999)
                     /locus_tag="VIJ44_08870"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY5166050.1"
                     /translation="LDGGVASQVDAVQQIARGNGAVSVRVAADDAERALLWKGRKSAF
                     GAIARIAPDYYLHDAVVPRTRLVDVLQQVYAIAAAQHLTMMNVFHAGDGNLHPLIVFD
                     AREPGIWERVTEAGDAILRACVDAGGVLSGEHGIGLEKRDAMPMVFGPDDLDAQARLR
                     DAFDPSGRANPDKVLPRGSRCGELMRVPDGAWI"
BASE COUNT          719 a         1697 c         1779 g          804 t
ORIGIN      
        1 cgatcaactg cgccgcgaca cggttggtga gaccgtcaca ttcgtccgca accggaacat
       61 caactacacg aacatctgca ccttcaagtg ccggttctgc gcgttctcga agggtcccct
      121 gtcgctgaac ctgcgcggcg acccgtacct cctcgatgtc ggtgagatcc aacgccgggt
      181 cgtcgaggcg gtcgagcacg gcgccaccga ggtgtgtctc caaggcggta tccatcctga
      241 cttcgacggc gcgtactacc tcggcgtcac ccgcgccgtg aaggatgtcg cccccgacat
      301 tcacgtgcac gggttcaccg cgttggaggt caccgagggg gcccgccgcc tcgggatacc
      361 gctgcgcgac tatctgcgtg agttgaaggc cgccggtctc tcgaccctcc cgggcaccgc
      421 ggcggagatc ctcgacgacg aggtgcgggc gatcatctgc cccgacaagg tcaccaccga
      481 tgagtggctc gacgctcatc gcgatgctca ctcggtgggg ctgcgctcca acgtgacgat
      541 catgttcggt tcggtcgagg caccggtcca cgtcgctcgt cacctgactc gcacccgctc
      601 cctccagaag gagaccggcg ggttcaccga gttcgtgccc ctgccgttcg ttcacatggc
      661 gacgccgatc tccttgcagg gccgcgcccg gcgcggaccg acgttccgcg aggtgctcct
      721 cctccacgcg gtgggccgta tcgcgtaccg gggatggatc gacaacatcc agatctcatg
      781 ggtgaaggtc ggcatccggg gggcgcaaca ggcgttgcag gccggctgca acgacctcgg
      841 cgggacgctg atggacgaga acatctctcg ggcggcgggc gcgtcgcatg gacaggagct
      901 cgacgacgat gggttcgcgg cgatcgttgg cccgctcggt cgaccgttgg agcagcgcac
      961 caccttgtac gggcggaccc ggaccgtcgg acgggcgctg cggccgccag tcccggagcc
     1021 gaccccggtc gcgttgaccg cccgcgccgc gctgtgagcg tgccggcatc ggatggttcg
     1081 accgtcgacg tcgtcggagt cggcaacgcg ctcgtcgacg tgctcagcct tgccaccgac
     1141 gaattcctcg acgaccatgg gctgaccaag ggcgcgatgg cgctcatcga cgccgaccag
     1201 gctgagcggc tctacgccgc gatgggaccc ggaacggaga tgtcgggggg ctcggcggcc
     1261 aacaccatgg ttggtgtgtc atcgctcggc ggaaccagcg ccttcgtcgg tcggatccgt
     1321 gacgacaccc tcggtcgggt gttcgcgcac gacatccagg cctccggggt ccggtacgac
     1381 actgcggcgg cgcccgctgg tcacccgacc gggcggtgct tgatcctcgt cacccccgac
     1441 gccgaacgga ccctcaacac ctttctcggc gccgcgtctg agctggcggt ggcggatgtc
     1501 tccgagacgc tggtgcgagg ggcgccggtc acctacctcg aggggtacct gttcgaccag
     1561 ccggatgcga aagaggcgtt ccggcacgcg gcgaggttgg cgcacgcggc gggacggcgg
     1621 gtggcgctca gtctctccga cacgttctgt gtcgagcgtc accacgccga cttcttggcg
     1681 ctggtcgagc atggagtcga catcctcttc ggcaacgagt cggagatccg cgccctctac
     1741 cgggtgaacc gcctcgacga cgcgttcgag ctggtggcac ggcactgcga gcttgcggcg
     1801 ttgacccgcg gggctcgggg ctcggtggtg gtcacccccg agggcatcca cgaagtcgag
     1861 gtgcacccgg tggcgggacc ggtcatcgac accaccggtg ctggtgacca gtacgcggcg
     1921 gggttcctgt tcgcgttcac gcacggctca gacccggtga cgtgcggccg ggtcggggcg
     1981 ctggcggcgt ccgaggtgat ctcccatttg ggggcgcgcc cggaacgccc gctggctgag
     2041 ctcgcggcgc cgctgctgcc gtgaagctcc cccggtaccg gaccggagac ccggagctgg
     2101 acgacgccgt cgcggcgctg gtcgagaggg tcgccgacga cgacgcggac ctggtgttcg
     2161 agctggtggt gtcggcgata cgactggccc gggaccacgc cgaccgcggg gacctgaaga
     2221 tggcgaacgc cgcgttgaag gagatgcggt actcgttcca cgtcttcgag ccgtaccgag
     2281 cggcccgcaa ggtcgcgatc ttcggatcgg cccgcaccca acccgacgat ccgctctacc
     2341 accaggcgcg gcgattcgcg gaggcagtcg cggcccggga ctggatggtc atcacggggg
     2401 ccggcccggg gatcatggaa gccggcatcg agggggcggg ccccgaccat gccttcggcg
     2461 tgagtatccg gctgccgttc gaagcggcgt cgacccagtt catcgcggac gatccgaagc
     2521 tgatcaactt ccggtacttc ttcacccgca agctggcgtt cgtcaaggaa gcggatgcgt
     2581 tcgtgctgct acccggcggg tacgggacca tggacgagac attcgagctg ctcaccctga
     2641 tgcagacggg gaaggcccaa ccggggccgg tggtgttgct cgacactccg ggtggcacgt
     2701 actgggaagg ctggctcgag ttcgtgcgcg gagagctcct caccaaccgg tacgtgtccg
     2761 gtcccgacct cgagctgttg ttcgtcaccg acgacgtcga cgccgcggtc ggtgaggtca
     2821 ccgatttctt cgcgaactat cactcgcaac ggttcgtgga gggtcgcctg gtgcttcggg
     2881 tccggcgagc gcctccgccc gcgaccctgg acgccctcag tgaggagttc cgcgacatcg
     2941 tgcggcgcgg ggcgatcgag cgggtcgacc cgtcaccggc tgagattgcc gacgatgacg
     3001 ctctggatct cgaacgggtc gcgttctggt tcgaccggca cggctgggcc cggcttcgag
     3061 agctcatcga ccggctcaac ggccacgagc cacacacgaa ctaaccgtcg ggttcggccc
     3121 cgagggcgcg cagggcccgt tcgactgcgc ggcgcgcctg ttgcagacgc cgctcttctt
     3181 tgaccgcgtc ttcgtcgcca tcggcggccg cgcgcagccg gtcgtaggcg gcgtcgcgga
     3241 gtcgctcttc gatgccgcgg agcgtctcga cgacgtcgcc gagtcccggt tcagacacga
     3301 tcaatcctcg gtgggctcgg ggacagcggc actgcgctgg tcggcgcggc cgggccggtc
     3361 atcgagccgg aggcggcagg gtcggggcgg ttcacgaagc ctcgcgttcg ggttggggga
     3421 ttggcagccg tcccggcgca agcttggctt ccgggtcgaa tgccatcttg atccgagcca
     3481 tgagtgcccc gttgggaaac tcgattccga atccgtcgag gccggccgca ccggcggttc
     3541 ggagcaacca accctgcgcg acgtgggccg cggcgcgggc ctcgctcagc gccgcctcgc
     3601 tcagcgcggc gacgtggacc gtcccgacgc cccactcggc ggtccacgtg acctcgccaa
     3661 tcgattccag ctcgtgaccg agctggctga tccggtcggg cgcgacggag atgcggccct
     3721 ggtggggtcc gtcgggaaac gtcggtgcct cgtcgagcgg gtcgagtgac gccgcacgtg
     3781 cttgggcgtc gacgtcgtcg gggtggccct cgaggaggac ctggctgcca gtgtccgggt
     3841 gccaaaagat ggcggacggc tggaagcacc gggctcgcac cgcggtgggc tcgaggtcgg
     3901 tgccgaacca gcggatgcac tgcggacggg gctggcagcg caggatgacc cgggtcagga
     3961 ttccgagcgt cccgaaggag ccgaccagga gccggggcat gtcgtagccg gtgacgttct
     4021 tgaccgtcgg tcccccaccc cgcacgaccc ggccggcggc ggtcacgaac cgcacctcga
     4081 ggacacggtc ccgaagcggc ccgtatcgga ggcgacggtg tccggacagg ccaaccgcga
     4141 ggagcccgcc gatggttccc gtcgggtcgc gcccgtcgag gacgcactct tggtggtcgg
     4201 cggccaacgt cgcgtcgagc tcaccgaccg tcgtcccggc ccaggcggtg acggtcagct
     4261 ccgcggggtc atagctcacg atccctcggg gcgcgtcgac gggcgtcgcg gcgatgactg
     4321 ggttgcctac ctcgcggtgc gttcctccgc cgaccgcgac gaccgtctcg gcgtcggcga
     4381 cgaccgcttg cacgtcggct tcggagcgct ggccagtcag atccatgcgc cgtccgggac
     4441 ccgcatcagc tcgccgcagc ggctgccgcg tgggaggacc ttgtcggggt tcgctcgccc
     4501 ggacgggtcg aaggcatcgc gcaggcgagc ctgcgcgtcg aggtcgtcgg gtccgaagac
     4561 catgggcatg gcgtcccgct tctcgagccc gatgccgtgc tcgcctgaca ggacaccgcc
     4621 cgcgtcgacg caggcccgca ggatcgcgtc tcctgcttca gtgacccgtt cccagatccc
     4681 cggttcgcgg gcatcgaaca cgatgagcgg gtggaggttg ccgtcgccgg cgtggaagac
     4741 gttcatcatg gtcaggtgct gcgccgcggc gatggcgtac acctgttgga ggacgtcgac
     4801 gagacgggtt cggggcacca ccgcgtcgtg gaggtagtag tcgggtgcga tgcgggcgat
     4861 ggcgccgaac gccgacttgc ggcccttcca gagcagcgcc cgttcggcgt catccgcggc
     4921 cactcgcacg ctcaccgcac cgttgccccg cgcgatctgt tgcacggcat ccacttggct
     4981 cgcgacgccg ccgtcgagg
//
LOCUS       DATCCH010000460         2323 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_408067, whole genome shotgun sequence.
ACCESSION   DATCCH010000460 DATCCH010000000
VERSION     DATCCH010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408067"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1022
                     /locus_tag="VIJ44_08875"
     CDS             <1..1022
                     /locus_tag="VIJ44_08875"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY5166051.1"
                     /translation="GWRRIVPVGAKTALKDLRELRRARAFRVAADGPWRGRDLAFVWQ
                     RHELFQTAGLRLASELRVPSVMFVPATLVWQARQWGVRRPGWSRAVEHFGERVPLRGA
                     DVVACGTEAVVAEVVRLGVDERRVVVTPTGVDTELFTPDASGTAVRAQLGLEHRFVVG
                     WVGSFRRFHALDQAVAALAGAEDVTLLLVGDGPERRRIEELGRAAGVHVVCTGTVDHR
                     RLQGYLAAMDVGLLLAAPGAAFHYSPLKLAEYLAAGIAVVAARAGELPEQLRDGTDAL
                     LVPPGDTVMLRTALLRLRDDPEERARLGRAARTAAVERWSWDRSIRRVLDHLADRGGG
                     ASGAG"
     gene            1151..1954
                     /locus_tag="VIJ44_08880"
     CDS             1151..1954
                     /locus_tag="VIJ44_08880"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY5166052.1"
                     /translation="MTGLPELRPYVASLAERRRFMWHLARTDLKAEHYDTALGQLWVI
                     LDPLLTAAVYFLFRSVIKPSGNAANRNFVLSHLVWGVFFFTYTNNAITGGARSVLQGR
                     NLILNASFPRVLLPIVAIIKSLLDFLPTIVVYFVLHAILRQPFGLSLIALPLLVLIQT
                     VFNLGIGMLLAPLMVFYRDTGGFLPYLTRLWLYVTPVLYTIAEIPKPLKAFLRWNPLY
                     PLFGALEPVFKGQFPPGTYVLGAAAWAIAFFIIGSIVFLARERDFAVRL"
     gene            2046..>2323
                     /locus_tag="VIJ44_08885"
     CDS             2046..>2323
                     /locus_tag="VIJ44_08885"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY5166053.1"
                     /translation="MRNRAKRSMLIEALRGVSFEVPVGSVYGVVGRNGAGKTTLFRTI
                     AGILPPTYGRVTVWGHVTPLLSLGVGFNRELTGRENILLGGLTAGLSP"
BASE COUNT          312 a          817 c          777 g          417 t
ORIGIN      
        1 ccggctggcg ccgcattgtc cccgtcgggg cgaagaccgc actgaaagat ctccgcgagc
       61 tgcggcgcgc gcgggccttc cgggtggcgg ccgacggtcc gtggcggggg cgagacctgg
      121 ccttcgtctg gcaacgccac gagctgttcc agaccgcggg actgcgactc gcctccgagc
      181 tgcgggttcc gtcggtcatg ttcgttccgg cgaccctcgt ctggcaggca cggcaatggg
      241 gtgttcgccg gccgggctgg agccgggcgg tcgagcactt cggcgagcgt gtcccgttgc
      301 gtggggccga cgtggtcgcc tgcgggaccg aagcggtcgt cgccgaggtc gtccggctcg
      361 gagtcgacga gcggcgggtc gtcgtcacgc ccacgggagt ggacacggag ctgttcaccc
      421 ccgacgcgag cggcacagcc gtgcgggcgc agctcgggct cgagcatcgt ttcgtcgtcg
      481 gctgggtcgg gagcttccgc cggtttcacg cactggacca ggccgtcgcc gcgcttgcgg
      541 gcgccgagga cgtcacattg ctcctggtcg gcgacggacc ggagcgccgg cgcatcgagg
      601 agctcgggcg ggcggccgga gtgcacgtcg tgtgcacggg aacggtcgac catcgccggc
      661 tgcagggcta cctcgccgcg atggacgtgg gcctgttgct cgcggcgccg ggagccgcgt
      721 tccactactc gccgttgaag ctggcggaat acttggcggc aggcatcgcg gtcgtcgcgg
      781 ctcgggccgg agaactgccc gaacagctcc gcgacgggac cgacgcgctg ctcgtgccgc
      841 cgggcgacac cgtgatgctc cgaaccgcgt tgctgcgatt gcgggacgat cccgaggaac
      901 gggcccggtt gggacgagcg gcacggaccg cagccgtcga gcggtggtcg tgggaccggt
      961 cgatccgtcg ggtgctggat catctggccg atcgtggcgg aggggcttcc ggcgctggct
     1021 agcatctgtc gccacgccgg gcgcgcccgc gcccgcatgg aaccccccgg ccggaacaac
     1081 cctgcctgga acacgtcgac agggcactat ggcagccgca tctcgaactg caacggtgta
     1141 cgagccgacc gtgaccggcc tgcccgagct acggccctat gtggcctcgc tcgccgagcg
     1201 acgccggttc atgtggcatc tcgcgcgcac agacctgaag gccgagcact acgacaccgc
     1261 gctcggccaa ctctgggtga tcctcgatcc tctgctcacg gccgccgtct acttcttgtt
     1321 ccgcagcgtc atcaagcctt cgggcaacgc ggccaaccgc aactttgttc tctcccacct
     1381 ggtgtggggc gtgttcttct tcacgtacac caacaacgcg atcacgggcg gggccaggtc
     1441 ggtcctccag ggccgcaacc tcatcctcaa cgcgtcattt ccgcgggttc tgctgcccat
     1501 cgtcgcgatc atcaagtcgc tgctcgattt ccttccgacg atcgtcgtct acttcgtgct
     1561 gcacgcgatc ctcaggcagc ccttcggcct gtcgctcatc gcactcccgc tcctcgtcct
     1621 cattcagacc gtcttcaacc tcggcatcgg gatgctcctc gccccgctca tggtctttta
     1681 tcgagatacc ggcgggttct tgccctacct cacgaggctc tggctgtacg tgacacctgt
     1741 gctgtacacg atcgccgaga tcccgaagcc gttgaaggcg ttcctccggt ggaatcccct
     1801 gtacccgctg ttcggcgcgc tcgaaccggt cttcaagggt cagttccccc cgggcacgta
     1861 cgtcctcggg gccgcggcct gggcgattgc gttcttcatc atcgggagca tcgtctttct
     1921 cgctcgggaa cgcgattttg ccgtccgtct ctgaggtccc gccggcggtt cgcgtcgaag
     1981 acctgtgggt gagctttcgc gccacgcgag agcgccgacc gacgctcagg ggcaccgtcg
     2041 ggagcgtgcg caatcgcgcc aaacggtcga tgctcatcga agcgctccgc ggcgtcagct
     2101 tcgaggttcc ggtgggctcc gtctacggcg tcgtcgggcg caacggtgcc ggaaagacca
     2161 cgctgttccg caccatcgcc ggcatccttc cacccacata cgggcgcgtg accgtctggg
     2221 gccatgtcac cccgctgctc tccctcggcg tcggtttcaa ccgcgagctg accgggcggg
     2281 agaacatcct cctcggcggg ctcaccgccg gcctctcccc cga
//
LOCUS       DATCCH010000461         4665 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_76571, whole genome shotgun sequence.
ACCESSION   DATCCH010000461 DATCCH010000000
VERSION     DATCCH010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4665
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76571"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1356
                     /locus_tag="VIJ44_08890"
     CDS             <1..1356
                     /locus_tag="VIJ44_08890"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEY5166054.1"
                     /translation="LSIARADNLFRGLGRADDAENIVRAAELRVVDDPVLRNGLVALR
                     ATFLLFEGRLTDAVHLVEPLLGEGADVAYAQGALVGSVAFALGGRPNEAVVVAARGEQ
                     ARIALGEQVQLAAPGIYLVARAQALLEAGQMRDATELAQLGYDGAVEMGAPHGQAWFA
                     SILGRIDLHRGKAGSAARWSREGGVVWRELRHPAARWGFGALATAQALTGDLDAAEAT
                     YAEVDAEPTTPVRVFDPEIDRGRASIAAQRGERTRACEILVDAADRAEAGGAFGIAAG
                     ALHDLARLGETRLAVSRLDAIAPHVDGDYMAARLEHAHALLADDPDRLDAVSEDFERI
                     GALLLASEAASSAGAMHRHAAMRRKATASAQRAVALLAECEGARPFGVEPEVPGVSLT
                     RREREVAELAATNLTSREIAEKLFVSTRTVENHLQRAYEKLGVRGRRELRQAIAPGGD
                     A"
     gene            1353..2516
                     /locus_tag="VIJ44_08895"
     CDS             1353..2516
                     /locus_tag="VIJ44_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013872367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY5166055.1"
                     /translation="MNPPTDPDVRAELIATVRRFVQRDVMPVASELEHADEYPADLVA
                     TMRHLGLFGVTIAEDYGGLGLDLLTYIGIIEELAAGWMSLSGILNTHVMVANLISASG
                     TDEQRARWLPSLAAGDVRGALSLSEPDAGSDTAALTCRAERDGDEYVITGTKMWVTNG
                     VRAGLVALAARTAEGITCFLIDKEPGDQFGGITVSRNIGKLGYRGIETVEMVYDGHRV
                     PAAAVLGGDAGLGRGRHFILAALEVGRINIAARAVGVARAAFEASIRYAQQRHTFGVP
                     IAEHQAIQLKLADMATKLEAARLLTRNAAERRSAGERTDVEAGMAKLFASEAAFEIAT
                     DAMRIHGGMGYTTELPVERYYRDAPLMIIGEGTNEIQRLVIARGLLRRHAVDE"
     gene            2582..3094
                     /locus_tag="VIJ44_08900"
     CDS             2582..3094
                     /locus_tag="VIJ44_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEY5166056.1"
                     /translation="MTVYDRPFGRCLEDFEIGDIYRHWPGKTITEYDDHLFCLITMNH
                     HPLHLDAWYAENETQFGKNVVVGNLVYSVVLGMSVPDVSGAAIANLEVEELKHQRPTF
                     HGDTIYAETKVLDRKASASKPDRGIVTVETRGFNQRGEEVVYFRRKVMVPTRDAAPPR
                     KRPYPLPENG"
     gene            3091..3522
                     /locus_tag="VIJ44_08905"
     CDS             3091..3522
                     /locus_tag="VIJ44_08905"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEY5166057.1"
                     /translation="MSPADPTCTVCRIVTGALSAHIVLDEPQAVAFLDHRPLFPGHTL
                     VVPRDHVVTLPDLPGDEVAPFFLAVQRVAAAMPDALDAAGTFVAANNIVSQSVPHLHF
                     HVVPRRPKDGLRGFFWPRHRYETPEEMAAVAERLRRRLRVL"
     gene            3543..4058
                     /locus_tag="VIJ44_08910"
     CDS             3543..4058
                     /locus_tag="VIJ44_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019629468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5166058.1"
                     /translation="MTDSGQLQGWFAGRIPEGWFSGPPDVLADREEILVVGILAEPDA
                     GPDADDETRHAARKARIQGHREDTRQHRMRIADEAEARFARKVAWGARCGADEVVFTS
                     LGVPVMTRLRMNERRVLDTLVHAGVARSRSEALAWCVRLVHENESDWIEQLRRALVHV
                     EQVRAEGPAAR"
     gene            4143..>4665
                     /locus_tag="VIJ44_08915"
     CDS             4143..>4665
                     /locus_tag="VIJ44_08915"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY5166059.1"
                     /translation="MSGGATLAFWGVRGSTPCAGPRYARYGGNTSCVSLEAEGHQPII
                     FDLGTGLREYGDAITARFTAELEANNLTSPYLATVLLTHLHWDHIIGLPFFTPAFRPD
                     ACVDIHGPEQRDGLGATFSGVMHPPYFPITPAEMGGTLTFIDTNADDFPVNGAKVRSR
                     WVRHTDPTLGFRVE"
BASE COUNT          715 a         1761 c         1521 g          668 t
ORIGIN      
        1 ctgtccatcg ctcgagctga caacctcttc cggggtctgg gtcgtgccga cgacgccgag
       61 aacatcgtgc gggccgccga gctccgcgtc gttgacgacc cggtgctgcg caacggtctc
      121 gtcgccctgc gcgccacctt cttgctgttc gagggacggc tcaccgacgc cgtccacctc
      181 gtcgagccgc tgctcggcga gggcgccgac gtcgcgtacg cccagggcgc actggtcgga
      241 tcagtcgcgt tcgcgctcgg cggacgcccc aatgaggcgg tcgtggtcgc agcccgggga
      301 gagcaggccc gcatcgccct cggcgaacag gtccagctcg ccgcgcccgg catctacctc
      361 gtggcccgcg cccaagccct gctcgaggcg ggccagatgc gcgacgccac cgagctcgcc
      421 cagctcggct acgacggcgc ggtcgagatg ggcgcccccc acggtcaagc ctggttcgcg
      481 tcgatcctcg gccgcatcga cttgcaccgc ggcaaggcgg gcagcgcggc gcggtggagc
      541 cgcgaaggcg gcgtggtgtg gcgcgagctg cgccaccccg ccgcgcggtg gggcttcggt
      601 gccctggcga cggcgcaggc cctcaccggc gatctcgatg ccgccgaagc cacctacgcg
      661 gaggtcgacg ccgagccgac gaccccggtg cgggtcttcg accccgagat cgaccgcggc
      721 cgcgcgtcga tcgccgcgca acgcggcgaa cggacccgag cgtgcgagat cctcgtcgac
      781 gcggcggacc gggccgaggc cggcggggcg ttcggcatcg ctgccggtgc gctccacgac
      841 ctcgcgcgcc tcggtgagac ccgactggcg gtctcgcgtc tcgacgccat cgcgccccac
      901 gtcgacggcg actacatggc cgcccgactc gagcacgccc acgccctcct cgccgacgac
      961 cccgaccgtc tcgacgccgt ctcggaggac ttcgagcgca tcggggcgct cctgttggcc
     1021 tccgaagccg cctcgtcggc gggcgcgatg caccgccacg ccgcgatgcg ccgcaaggcc
     1081 accgcctcgg cccaacgggc cgtggcactg ctcgccgagt gcgaaggggc ccgaccgttc
     1141 ggtgtggaac ctgaggttcc cggcgtgtcc ctcacccgac gggaacgaga ggtcgccgag
     1201 ctcgcggcga ccaatctgac cagccgggag atcgccgaaa agctgttcgt cagcacccgc
     1261 accgtcgaga accatctgca acgggcctac gagaagctgg gggtccgggg ccggcgcgag
     1321 ctgcgccaag ccatcgcccc cgggggagac gcatgaaccc gcccaccgat ccggacgtcc
     1381 gcgccgagct gatcgccacg gtgcgccgat tcgtccaacg agacgtgatg cccgtcgcct
     1441 cagagctcga acatgccgac gagtacccgg ccgacctcgt cgccaccatg cgccacctcg
     1501 ggctgtttgg cgtcaccatc gccgaggact acggcgggct cggtctcgac ctgctcacct
     1561 acatcggcat catcgaagag ctcgccgcgg gctggatgtc cctgtcggga atcctcaaca
     1621 cccatgtgat ggtggccaat ctcatcagcg ccagcggcac cgatgaacaa cgcgcccgct
     1681 ggctcccgtc actcgccgcc ggcgacgtgc ggggcgcgtt gtcgctctcc gagcctgacg
     1741 cgggcagcga caccgcggcc ctcacctgtc gggcggaacg cgacggcgac gagtacgtca
     1801 tcaccggcac caagatgtgg gtgacgaacg gcgtccgggc ggggctcgtc gctctcgccg
     1861 cccgcaccgc cgaaggcatc acgtgcttcc tgatcgacaa ggagccgggc gaccagttcg
     1921 gtggtatcac cgtcagccgc aacatcggca agctcggcta ccgggggatc gagaccgtgg
     1981 agatggtcta tgacggccat cgtgtccccg cggccgcggt gctcggcggc gacgccgggc
     2041 tcggacgtgg ccgccatttc atcctcgccg cgctcgaggt cggtcgcatc aacatcgcgg
     2101 cacgcgccgt cggggtcgcc cgggccgcgt tcgaggcgtc gatccggtac gcgcagcaac
     2161 gccacacgtt cggggtcccg atcgccgagc atcaagccat ccagctcaag ctggccgaca
     2221 tggccaccaa gcttgaagcg gcgcgtctcc tgactcggaa cgccgccgag cggcgcagcg
     2281 ccggtgagcg caccgacgtg gaggccggca tggcgaaact gttcgccagc gaagccgcct
     2341 tcgagatcgc caccgacgcg atgcggatcc acggtggcat ggggtacacg accgagctcc
     2401 cggtcgagcg ctactaccgc gacgccccac tgatgatcat tggcgaaggt acgaacgaga
     2461 tccaacggct ggtcatcgcc cgcggtctgc tgcgccgaca cgccgttgac gagtaagcac
     2521 cggccaccga ccggaggccg accgcggcac gcccccggaa cccctcggta cgcttcgagc
     2581 catgaccgtc tacgaccgcc cgttcggccg gtgcctcgag gacttcgaga tcggcgacat
     2641 ctatcggcac tggccgggca agacgatcac cgaatacgac gaccacctct tctgtctcat
     2701 caccatgaac caccatccgc tgcacctcga tgcctggtat gccgagaacg agacccagtt
     2761 cggcaagaac gtcgtggtcg gcaacctcgt gtactcggtc gtgctcggca tgagcgtccc
     2821 cgacgtcagc ggcgccgcga tcgccaacct cgaggtcgag gagctcaagc accagcggcc
     2881 gaccttccac ggagacacga tctacgccga gaccaaggtg ctggaccgca aggcgtcggc
     2941 gagcaaacct gaccggggga tcgtgaccgt cgagacccgg ggcttcaacc agcgcggtga
     3001 ggaggttgtt tacttccgcc gcaaggtcat ggtccccacc cgggacgcgg cgccgccccg
     3061 gaaacgtccg taccccctgc ccgagaacgg gtgagtcccg ccgaccccac ctgcacggtc
     3121 tgtcgcatcg tcaccggcgc gcttagcgcc cacatcgtcc tcgacgagcc ccaggccgtg
     3181 gcgttcctcg atcaccgccc cctcttcccc gggcacaccc tcgtcgtccc ccgcgaccac
     3241 gtcgtgacgc tccccgacct ccccggggac gaggtcgccc ccttcttcct ggccgtccag
     3301 cgggtcgccg ccgcgatgcc cgacgcgctc gacgccgctg ggaccttcgt cgccgccaac
     3361 aacatcgtca gccagagcgt cccgcacctg cacttccacg tggtgccccg ccgcccgaag
     3421 gacgggcttc ggggcttctt ctggccccgc caccgctacg aaacccccga ggagatggcc
     3481 gccgtcgccg agcggctgcg ccgccgcttg cgggtcctgt agtggtgtaa taatgtaatc
     3541 gcatgacgga ttcgggacag ctgcagggtt ggttcgccgg gaggatcccc gaggggtggt
     3601 tcagcggacc gcccgacgtt cttgccgacc gggaggagat cctcgttgtc ggcattctgg
     3661 ccgaacccga cgcgggccca gacgccgacg acgagacccg acacgcggcc cgcaaggccc
     3721 gtatccaagg ccaccgcgag gacacccgcc agcaccggat gcggatcgcc gacgaggccg
     3781 aggcccggtt cgcccgcaag gtcgcctggg gtgcccgctg cggcgccgac gaggtcgtct
     3841 tcaccagcct cggggttccc gtcatgaccc ggctgcgcat gaacgagcgc cgggtgctcg
     3901 acaccctcgt ccacgccggc gtcgcccgca gccgatccga ggcgctcgcc tggtgcgtcc
     3961 ggctcgttca cgaaaatgag agtgactgga tcgaacagct gcgccgcgcc ctcgtccacg
     4021 tcgagcaggt ccgcgccgaa gggcccgccg cccgctgaaa ctcgtcttgc tccgggtcgc
     4081 gacgtcgcgg tgggtggtgc ctcgctgacg gtgagggcac ccggaggggt agggtcctgg
     4141 cgatgtcggg gggagccacg ctggcttttt ggggcgtccg gggctcgacc ccgtgcgcgg
     4201 gtcctcgcta cgcgcgctac ggcggcaaca cctcgtgtgt ctccctcgag gctgagggcc
     4261 accagccgat catcttcgac ctcgggactg gcctgcgcga gtacggcgac gcgatcacgg
     4321 cccgcttcac cgccgagctc gaagcgaaca acctcaccag cccgtacctc gccaccgtcc
     4381 tgctcaccca cctccactgg gaccacatca tcgggctgcc gttcttcacg ccggcgtttc
     4441 gccccgacgc ttgcgtcgac atccacggcc ccgagcagcg cgacggcctc ggcgccacct
     4501 tcagcggggt gatgcatccc ccgtactttc cgatcacccc ggcggagatg ggcggcaccc
     4561 tcacctttat tgacaccaac gccgacgact tcccagtcaa cggggccaag gtccgctccc
     4621 gctgggttcg ccacaccgat cccaccctcg ggttccgcgt cgagc
//
LOCUS       DATCCH010000462         2323 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_408200, whole genome shotgun sequence.
ACCESSION   DATCCH010000462 DATCCH010000000
VERSION     DATCCH010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408200"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="VIJ44_08920"
     CDS             complement(<1..554)
                     /locus_tag="VIJ44_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY5166060.1"
                     /translation="MTLHVEHRDRVQVLTLDDPDRRNALSASMVDEIVAAVEAAEVDD
                     GTGAIVVTGAAPAFCSGADTSNLRALGDAEPSDSGSVRSLYDGFLAVRDCTLPTVAAV
                     NGPAVGAGFNLALCCDVRMVGESARFDSRFMTIGIHPGGGHTWMLERAVGPQAAAAMV
                     VFGERIEGRRAAELGLAWRCVPDG"
     gene            complement(551..1585)
                     /locus_tag="VIJ44_08925"
     CDS             complement(551..1585)
                     /locus_tag="VIJ44_08925"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY5166061.1"
                     /translation="MRILVTGLGSFWGSKVAQRLENEPGVELVVGVDTQEPVVALEHT
                     EYVRTDSSFSILERIVRATKVDTILHTHLIVDSTKDSGRALHEINVIGTMNLLAAAGA
                     AGSTIRKVVLKSATLEYGSSYDDPYFFRETMRRSHGPKTRVERSLLEAASVVGDFAED
                     NPHVTVTKLDFANVLGDHVSSPLARMLRLPAVPEVFGFDPRLQFVHEDDVIGALAFAT
                     RRDVPGVFNVAGDGTMPWSEVCAIVGKRRVPMPPVLTSLAAEPLRLLRLVEIPPEVLA
                     LLRYGRGVDTTAFVQAGYQYGYSTAGTVDAFARGLRLQTTVGQPAYRYERDVENFFRH
                     SPAVIRDGET"
     gene            complement(1585..>2323)
                     /locus_tag="VIJ44_08930"
     CDS             complement(1585..>2323)
                     /locus_tag="VIJ44_08930"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY5166062.1"
                     /translation="VARRLYDPIYRRWFRAEWEGLEHIPAEGGALLVGNHGGAIPSDA
                     PVVMHGIETELGRPVYGMADNLFRLVPVVGTLWSRAGGVPAHPDNAYRLLHDEQQLAL
                     VFPEGTKGTGKLARDRYQLRRFGRGGFVEIAMRAGVPVVPLAVIGNEEAMPIVYKASR
                     LARLMNLPYFPVTANQFVFGPVLGLVMPLPSKFRIRVLPPVYFDAAPNQERYNRSQVM
                     DQAERIRTHIQEALYEMLRNRRSVWFG"
BASE COUNT          389 a          842 c          748 g          344 t
ORIGIN      
        1 tcgccgtcgg gtacgcagcg ccacgccaaa cccagctcgg cggcgcgtcg cccctcgatc
       61 cgctccccga acaccaccat cgccgcagcc gcctgcgggc cgacggcccg ctcgagcatc
      121 caggtgtgcc cgcccccggg gtggatcccg atggtcatga agcgggaatc gaagcgggcc
      181 gattcgccga ccatgcgcac gtcacaacac aacgccaggt tgaacccggc accgaccgcc
      241 ggtccgttca ccgcggcgac ggtcgggagc gtgcagtcac gcaccgccag gaacccgtcg
      301 tagagggacc gaaccgaccc cgagtcgctg ggctcggcgt cgccgagggc ccggaggttc
      361 gacgtgtcgg ccccagaaca gaacgccggt gccgcgcccg tgacgacgat cgccccggtg
      421 ccgtcgtcga cctccgccgc ctcgaccgcg gcgacgatct cgtcgaccat cgaggcgctc
      481 aaggcgttac gccggtcggg atcgtcgagg gtcaggacct gtacccggtc ccgatgctcg
      541 acgtggaggg tcatgtctcg ccgtcgcgga tcacggcggg ggagtggcgg aagaagttct
      601 caacgtcgcg ctcgtagcgg tacgccggtt gaccgaccgt cgtctgaagg cgcagccccc
      661 gggcgaacgc gtcgacagtg ccggccgtcg agtagccgta ctggtagccg gcctgcacga
      721 acgcggtggt gtccacgcca cgcccgtagc gcagcagcgc caggacctcg ggtgggatct
      781 cgacgaggcg gagcagtcgg agcggctccg cggcgaggct ggtcagcacc ggtggcatcg
      841 gcacccgccg tttcccgacg atcgcacaca cctcgctcca gggcatcgtg ccgtcaccgg
      901 ccacgttgaa gaccccgggc acgtcgcgcc gcgtcgcgaa ggccaacgcg ccgatcacat
      961 cgtcttcgtg cacgaactgg agcctcgggt cgaacccgaa gacctcgggc accgccggga
     1021 ggcgcagcat gcgagccagc ggcgacgaga cgtggtcccc gaggacattg gcgaagtcga
     1081 gcttggtcac cgtgacatgc gggttgtcct cggcgaagtc accgactacc gaggccgcct
     1141 cgagcagcga cctctcgacg cgtgtcttcg gtccgtggga gcggcgcatc gtctcgcgga
     1201 agaagtacgg gtcgtcgtag ctcgacccgt actccagtgt ggcgctcttc aacaccacct
     1261 tgcggatcgt gctgcccgcc gcccccgccg ccgcgagcag gttcatcgtc ccgatgacgt
     1321 tgatctcgtg caacgcccgc ccgctgtcct tcgtcgagtc gacgatgagg tgggtgtgca
     1381 agatcgtgtc caccttcgtc gcccgcacga tccgctccag gatcgagaac gacgaatcgg
     1441 ttcgaacgta ctcggtgtgc tccaacgcga cgacgggctc ttgggtgtcg acaccaacca
     1501 ccagctccac accgggttcg ttctcgagcc gctgggcgac cttgctgccc cagaacgacc
     1561 cgaggccggt gacgaggatg cgcatcagcc gaaccacacg ctgcgccggt tccgcagcat
     1621 ctcgtagagg gcttcttgga tgtgggtgcg gatccgctcg gcctgatcca tgacctgact
     1681 gcggttgtac cgctcctggt tcggagcggc gtcgaagtag accggcggca gaacccggat
     1741 gcggaacttc gacggtagcg gcatcacgag cccgagcacc ggcccgaaca cgaactggtt
     1801 cgccgtcacc gggaagtacg gcaggttcat caacctcgcc agtcggctgg ccttgtacac
     1861 gatcggcatc gcctcctcgt tgccgatcac cgccagcggc acgaccggca ccccggcccg
     1921 catcgcgatc tccacgaacc cgcctcggcc aaaacggcgc agctgatacc gatcccgcgc
     1981 cagcttcccc gtccccttcg tcccttccgg gaacaccagt gccagctgct gctcatcgtg
     2041 gagcagccgg tacgcattgt ccggatgagc cgggacacca cccgcccggg accacagggt
     2101 gcccaccacc ggtaccaggc ggaacaggtt gtcggccatc ccgtagaccg gacggcccag
     2161 ctcggtctcg atgccgtgca tcaccaccgg cgcatcggaa gggatcgcgc caccgtggtt
     2221 cccgactagc agcgcaccac cctcggcggg gatgtgctcg agcccctccc actccgcacg
     2281 gaaccagcgc cgatagatcg ggtcatagag ccttcgggcg acc
//
LOCUS       DATCCH010000463         2200 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_408202, whole genome shotgun sequence.
ACCESSION   DATCCH010000463 DATCCH010000000
VERSION     DATCCH010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408202"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            604..1284
                     /locus_tag="VIJ44_08935"
     CDS             604..1284
                     /locus_tag="VIJ44_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY5166063.1"
                     /translation="MAHRVLLAEDDRATRESLVRALELDGYEVRAVGDGAAALDAFAA
                     EHPDVVVLDLMMPHVDGLTVCRRLRARNHQAPILIATARTEVSDRVSGLDAGADDYLP
                     KPFALDELLARIRALLRRATYETPDRELRVADLRVDEAARRVWRGGRELELTKTEFDL
                     LQLLARNAGIVLSHSTIYERIWGYDFGSDSKSLAVYIGYLRRKTEEHGASRLIQTVRG
                     VGYTLRDA"
     gene            1281..>2200
                     /locus_tag="VIJ44_08940"
     CDS             1281..>2200
                     /locus_tag="VIJ44_08940"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEY5166064.1"
                     /translation="MTLRARLALSLAVLAALSVVIVSVASYVATDRRLHDDVDAALIG
                     YATRLSDPDGRSAHMLCDTLSQSRVVPDGSSFQDFGGVLAGLPEGALECVSSSGHVLA
                     WTGAVELTVDASTRHSANRYPSSENERGTPLSPPTASGALTPAPSIPQPGSSTTTAAT
                     RALISSPRTVSVDGSPYRLVVVSVPGGGAIQIARSLDNTNHVLGSIRDLALAVGVAVI
                     ALAALAGWVIARRTTRPVLRLTDAAEAVGATGQLEIPVPPGGKDEVGRLARAFGSMLD
                     ALNRSRTQQQRLVQDAGHELRTPLTSLRAN"
BASE COUNT          315 a          789 c          753 g          343 t
ORIGIN      
        1 ccgggggaag ccggcccacc cgcacggtgg ggcggcggcc agcacggacc caaaggatca
       61 gctcgggatc cggccggccg accgtggtta ggcgaccgtc ggccgctagc cagcgtcctc
      121 ttgggcttcg cgctgcgcgc tctcgccctt ctcgtgggtc gcgtcctcgt tcggcttgaa
      181 cgttccgctc ggtgcgccgt tggtcgcggc gggcgccgtc gtggacgagc cgttggtctg
      241 gcactttctg agcccgcacc gaacgcggca acgccgatgg cgccgccgcc gagcatcgat
      301 cccgccatcg cggtcgcgag gacggccgtt cgagtgagat tcacatgcat ctcccgttcc
      361 tgggctgaca ggtcgcctcc gagatcagag gttgcgcttg accctgacgg gacgaggtaa
      421 ggccgccgct gaaccgactc aaaggatcgg acaagaacgt gaagcctgct cacgccacga
      481 ggtggcgccg cgacgcgcgg cgggtcccgt tcgatccccg gcgacgacgt cgccgcaccg
      541 gtccgactcc aacggagccc cgttccttac cctggtctca cctagacggg atagaccacc
      601 catatggcgc accgcgtctt gttggctgaa gatgaccggg ccacccgaga gtcgttggtg
      661 cgcgcgctgg aactcgacgg atacgaggtc cgagcggtcg gggacggcgc ggcggcgctg
      721 gacgcgttcg cggcggagca ccccgatgtc gtcgtgctgg acctgatgat gccgcacgtc
      781 gacgggctga ccgtctgtcg gcgtctccgg gcccgcaacc accaagcccc gatcctcatc
      841 gcgacggccc ggaccgaagt gtccgatcgg gtgtcgggtc tcgacgccgg cgccgacgac
      901 tatctaccga agccgttcgc gctggacgag ctcctcgccc ggattcgggc cttgctgcgc
      961 cgagcgacgt acgagacacc cgaccgggag ctgcgggtgg cggacctgcg cgtcgacgag
     1021 gcggcccggc gggtctggcg tggcggccgg gagctcgagc tgacgaagac cgagttcgac
     1081 ctgttgcagc tcttggctcg caacgccggg atcgtgctga gccattcaac gatctacgaa
     1141 cggatctggg gctatgactt cgggtccgac tcgaagtctc ttgccgtcta catcggctac
     1201 ctgcgccgca agaccgaaga gcacggtgcg tcgcgtctca tccaaacggt ccgcggggtc
     1261 gggtacacgc tgcgagacgc atgacgctcc gggcgcggct ggcgctgtcg ttggcggtgt
     1321 tggccgccct gtcggtggtc atcgtgtcgg tggccagtta cgtggccacc gaccgtcgcc
     1381 tccacgacga tgtcgacgcc gcgctcatcg ggtacgccac ccggctctcg gatccggacg
     1441 ggcgctcggc gcacatgctc tgcgacacgt tgagtcaatc gcgcgtggtc ccggacggca
     1501 gctcgttcca ggacttcggt ggcgtgctcg ccggtctgcc cgaaggagcg ctggagtgcg
     1561 tgagcagcag cggtcacgtc ctggcctgga ccggcgcggt cgagctcacc gtcgacgcct
     1621 cgacgcgtca ctcagccaac cggtacccat cctccgagaa cgagcgggga accccgctct
     1681 caccgcccac cgcctcaggc gcgctgacgc ccgcgccgtc gataccgcaa cccgggtcgt
     1741 cgacgacgac cgccgcgacg cgggccctga tctcctctcc ccggacggtc agtgtcgatg
     1801 ggagccccta ccggctggtc gtcgtttccg tcccgggcgg cggcgcgatc cagatcgccc
     1861 gcagcctcga caacacgaac cacgtgctcg gctcgatccg tgatctggcc ctcgcggtcg
     1921 gcgtcgcggt gatcgccctg gccgcgctcg cgggctgggt catcgcgcgg cggacgaccc
     1981 ggccggtcct gcgcctcacc gacgccgccg aggctgtcgg cgccaccggg cagctcgaga
     2041 tccccgttcc accgggagga aaggacgagg tcgggcgact cgcccgggcg ttcgggtcga
     2101 tgctcgacgc tctcaaccgg tcccgcaccc agcagcagcg gctggtccaa gacgccggcc
     2161 acgaactgcg caccccgctg accagcctgc gggccaacat
//
LOCUS       DATCCH010000464         2126 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_77573, whole genome shotgun sequence.
ACCESSION   DATCCH010000464 DATCCH010000000
VERSION     DATCCH010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77573"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VIJ44_08945"
     CDS             complement(<1..324)
                     /locus_tag="VIJ44_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016700211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY5166065.1"
                     /translation="MTTRVELVTTSGVFSLDGDDFEVENNIWIVGDDDEVLIVDAAHD
                     AAPIAEGVGDRMVVAIVCTHGHNDHINAAAALAQRVGRPPVLLHPDDRMLWDVVYPDT
                     PPDGSL"
     gene            complement(321..1412)
                     /locus_tag="VIJ44_08950"
     CDS             complement(321..1412)
                     /locus_tag="VIJ44_08950"
                     /EC_number="1.1.1.306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020546874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050607 - mycothiol-dependent formaldehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0010127 - mycothiol-dependent
                     detoxification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)mycothiol dehydrogenase"
                     /protein_id="HEY5166066.1"
                     /translation="MESEVRGVVSAATGAPVTVETILVPDPGPGEAVVRVQACGVCHT
                     DLHYREGAITDAFPFLLGHEAAGIVEAVGDDVVGVAPGDFVVLNWRAVCGTCRACRRG
                     RPWYCFDTHNASQPMTRADGTPLSAALGIGAFAERTLVHAGQCTPVDRRVKPEAAGLV
                     GCGVMAGFGAATFTGGVGRGDTVAVFGCGGVGDAAIAAAVLAGARQVIAVDVDARKLG
                     WAREFGATDVVDAGQTDPVARIRDLTGGLGADVCIEAVGNPAVLEQAFYARDLAGTVV
                     QVGVPSPEMMLPAIPMIDFFGRGGALKPSWYGDCLPSRDFPALVDLAVQGKFPLDRFV
                     SETIPLDEVEEAFARMERGEVLRSVVVFE"
     gene            1507..>2126
                     /locus_tag="VIJ44_08955"
     CDS             1507..>2126
                     /locus_tag="VIJ44_08955"
                     /inference="COORDINATES: protein motif:HMM:NF014827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HEY5166067.1"
                     /translation="MEITTVMSEHGHEQVVLVADPASRLRAVIAIHSTALGPSLGGIR
                     FWRYDHDDDGIRDALRLAEAMTWKAAVAGLDQGGGKAVVFVDDPDAPRPAGLLQALGR
                     AIDTLGGRYLAAEDVGATTRDMDLIATVTPWVTGVSEADGGSGDPSPVTARGVLHAIR
                     AVVRARDGADATLAGCRVAIQGAGKVGAHLARLAVEAGASVVVADL"
BASE COUNT          320 a          779 c          736 g          291 t
ORIGIN      
        1 gaggctgccg tcgggtggtg tgtcgggata caccacgtcc cagagcatcc ggtcatcggg
       61 gtgcaacagc accgggggcc gaccgacgcg ctgggcgagc gccgcggcag cgttgatgtg
      121 gtcgttgtgc ccgtgggtgc acacaatcgc gacgaccatg cgatcgccga cgccctcggc
      181 gatgggggcg gcgtcatggg ccgcgtcgac gatgaggacc tcgtcgtcgt cgccgacgat
      241 ccagatgttg ttctcgacct cgaagtcgtc accgtcgagg gagaagaccc cgctcgtggt
      301 gacgagctcg actcgggtcg tcattcgaac accaccaccg atcgcagcac ctcaccgcgc
      361 tccatgcgag cgaaggcctc ctcgacctcg tcgaggggga tggtctcgga cacgaaccgg
      421 tccaacggga acttgccctg caccgcgagg tcgacgaggg ccgggaagtc gcgagacgga
      481 aggcagtccc cgtaccacga cggcttgagc gcaccgcccc gaccgaagaa gtcgatcatg
      541 gggatcgccg ggagcatcat ctcggggctc gggaccccga cctgcaccac cgtcccggct
      601 aggtcgcggg cgtagaaggc ctgctcgagc acggccgggt tgccgaccgc ctcgatgcac
      661 acatccgccc ccaacccgcc ggtcaggtcc cggatccgcg ccaccggatc cgtctgtccg
      721 gcgtcgacga cgtcggtggc cccgaactcc cgggcccagc cgagcttgcg ggcgtcgaca
      781 tcaacggcga tcacctggcg ggccccggcg agcacggccg cggcgatcgc ggcgtcgccg
      841 accccgccgc agccgaacac cgccaccgtg tcgcctcgcc cgacgccacc ggtgaaggtc
      901 gcggccccga acccggccat caccccgcag ccgacgagac cggccgcttc gggtttgacg
      961 cgccggtcaa ccggggtgca ctgaccggcg tggaccagcg tgcgttccgc gaacgccccg
     1021 atgcccagcg cggcgctgag tggtgtcccg tcggcccggg tcatcggctg cgacgcgttg
     1081 tgggtgtcga agcagtacca agggcgtccg cgccgacacg cccgacacgt tccgcacacg
     1141 gcccgccagt tcaacaccac gaagtcaccc ggcgcgacgc ccacgacatc gtcaccgacc
     1201 gcttcgacga tgccggccgc ctcgtgcccg aggaggaacg ggaacgcgtc ggtgatcgcg
     1261 ccctcgcggt agtgcaggtc ggtgtggcac accccgcacg cctggacccg cacgacggct
     1321 tcaccgggcc caggatccgg gacgaggatc gtctcgaccg tcaccggggc gccggtcgcg
     1381 gcgctgacca cacctcgcac ctcactctcc atgtcggtcc tccgtctcgc cgtcggtgtg
     1441 tcggcccgtc cgctcgatgc tcgggcagcg aacctaaccg gagcgcaccg cgtagcgtca
     1501 cggtgcgtgg agatcacgac cgtcatgagc gaacacggcc atgagcaggt ggtgctcgtc
     1561 gcggatcccg cttcacgtct gcgggccgtc atcgcgatcc actcgaccgc gctcggtccg
     1621 tccctcggtg ggatccgctt ctggcgctac gaccacgacg acgacgggat ccgcgacgcg
     1681 ctgcgcctcg cagaggcgat gacctggaag gccgcggtcg ccggactgga ccaggggggc
     1741 gggaaggcag tcgtgttcgt cgacgatccc gacgccccac gtccggcggg gttgctgcag
     1801 gctttgggcc gggccatcga caccctcggc ggccgatatc tcgccgccga ggacgtgggt
     1861 gccaccaccc gggacatgga cctcatcgcg acggtcaccc cgtgggtgac cggggtgagc
     1921 gaagccgacg gtggatcggg cgatccgtcg cccgtcaccg cgcgcggggt cctgcatgcc
     1981 atccgggctg tggtgcgcgc ccgggacggg gccgacgcca cgctcgccgg gtgtcgcgtc
     2041 gcgatccaag gcgccgggaa ggtcggagcc cacctggccc gcctcgccgt cgaagccggc
     2101 gcgtcggtgg tggtcgccga cctcaa
//
LOCUS       DATCCH010000465         2868 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_33748, whole genome shotgun sequence.
ACCESSION   DATCCH010000465 DATCCH010000000
VERSION     DATCCH010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33748"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="VIJ44_08960"
     CDS             complement(<1..505)
                     /locus_tag="VIJ44_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005460067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5166068.1"
                     /translation="MADLVARRAARQGALWGLVFGAVIVSSVSGYVSAYPTPTARARL
                     ETSLGTNPGLQALLGPARRISTVGGFTAWRGLGLVMLVGAVWGMLAATRLVRGEEEAG
                     RWEILLAGQTTRQRAAAQALVGLAAGWGALFATTAVVVVADGQSSDARFSVSASLFFA
                     VSLVASAG"
     gene            complement(558..1484)
                     /locus_tag="VIJ44_08965"
     CDS             complement(558..1484)
                     /locus_tag="VIJ44_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003985641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5166069.1"
                     /translation="MAVRTDGLSKRFGANEVLRALDLEVPRGEVLGYLGPNGSGKTTT
                     IRLLLGLLRPSAGRAEIFGFDSQRQAVEAHRRLAYVPGEASLWPSLTGAETLHLLGRV
                     QGSLDRAYRDLLVERFQLDPSKKVRAYSKGNRQKLALIAAFMARPDLLVLDEPTNGLD
                     PLMEQAFRACVAEARSEGQTVFLSSHILSEAEALCDRVGILRDGELVEIGTLTEMRHL
                     SALSVEATFDSAPPDLSRIAGVTVVEVDDHRVRCQVHGTVEPLLKVLAASGVHELLSR
                     EPSLEELFLAHYGARSAPAAAAAATAKSGHEH"
     gene            complement(1841..2398)
                     /locus_tag="VIJ44_08970"
     CDS             complement(1841..2398)
                     /locus_tag="VIJ44_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006378463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEY5166070.1"
                     /translation="MAVGYLEDLESRPAHLATDAVLRLASALDTTPGALLGGDVDRVP
                     GPGRSAARRGLDALSREEAQRRLATGGVGRLVFHAAKRGPVALPVNYLLVDGQVVFRT
                     ATDTSLAAAAGQDPVSFEVDHIDEAMSEGWSVLVTGRLERIEDPTERRRLVERGPTPW
                     AGEDRSVVLRVVVSDISGRRISTGW"
     gene            complement(2506..>2868)
                     /locus_tag="VIJ44_08975"
     CDS             complement(2506..>2868)
                     /locus_tag="VIJ44_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5166071.1"
                     /translation="RAEVGPMSVSPRKFGLPPRALEVPVAWQAAEHPALFPTLNGQLR
                     IRDAEHETIELRLTGEYTPPLGAIGVIGDRFAGHQAATASLRGYLVEVAQRLGAKLAE
                     HTPPLSPPPSRTPLERSS"
BASE COUNT          456 a         1061 c          952 g          399 t
ORIGIN      
        1 cgcccgccga tgcgaccagc gaaacggcga agaacagcga cgcgctgaca ctgaagcgcg
       61 catcggacga ctgtccgtcc gcgaccacca ccacggcggt ggtcgcgaac agcgcccccc
      121 agccggcagc cagcccgaca agggcctggg ccgcggcgcg ctgtcgcgtc gtctggccgg
      181 ccaggaggat ctcccaccgg ccggcttcct cctcgccccg caccaggcgc gtggcggcca
      241 gcatccccca gaccgctccg acgagcatga ccagcccgag gccgcgccac gcggtgaagc
      301 cgccgaccgt gctgatacgc cgggctggac cgagcaaggc ctgcaagccc gggttcgtgc
      361 ccagcgacgt ctccaggcgg gcccgtgcgg tcggcgtcgg ataggcactg acgtagcccg
      421 agaccgacga cacgatcacc gcgccgaaca ccagacccca cagtgccccc tggcgggccg
      481 ctcggcgcgc gacgaggtcc gccaccatcc aagcggagcg acctcccgcc ggcgcgcggc
      541 gcatcgccag actcgtgtca gtgctcatgg cccgactttg cggtagcggc agcggcggcg
      601 gccggcgcgc tcctggcgcc gtagtgcgcg aggaacagct cctcgagtga cggctcccgg
      661 ctcaacagct cgtgcacgcc tgaggccgcc agcaccttca ggagcggctc gacggttccg
      721 tggacctggc agcggacgcg gtggtcgtcg acttcgacca cggtgacccc ggcgatccgg
      781 gataggtctg gtggcgccga atcgaaggtc gcctcgaccg acagggccga caggtgtcgc
      841 atctcggtca gggtcccgat ctcgaccaac tccccgtctc gaaggatccc gacccgatcg
      901 cagagtgcct cggcctcgct gaggatgtgc gacgacaaga acacggtctg gccctcgctt
      961 cgcgcctcgg cgacgcacgc ccgaaacgcc tgttccatca gcgggtccag cccgttggtc
     1021 ggctcgtcga gcaccagcag gtcagggcgg gccatgaacg cggcgatcag ggccagcttc
     1081 tggcggttgc ctttcgagta ggcccgcacc ttcttggacg gatcgagctg gaaccgctcc
     1141 accagcagat cccgataggc gcggtccaac gagccctgca ctcgaccgag caggtgcagc
     1201 gtctcggccc cggtcagcga cggccacagg cttgcctcgc cgggcacgta cgccaagcgc
     1261 cgatgagcct caaccgcctg gcgctgactg tcgaacccga aaatctcggc tcgcccggca
     1321 ctggggcgca acaggcccaa cagaagccgg atcgtcgttg tcttccccga gccgttcggg
     1381 cccagatagc ccaacacctc gcctcggggc acctccagat ccaaggctcg gagcacctcg
     1441 ttggccccga aacgcttgga gagcccgtcg gtgcggaccg ccatctcact cacgagtctc
     1501 ccttcgtcgc gccgaccggc tccggacctc aggggcagga ccccgccacc gcccgatcga
     1561 accagtgggc gatcactctc cctggtccgt gcactgggca tgacaattcg cgagtgcccg
     1621 ctgggtccgc gacgctaccc agcccgcccg ggcgaactct gacgtcttcg ggtgtttcga
     1681 cgcgcgcggg ccgcgggcgc gtcaacgacc gcccacggtc aaacgtgacg cgtgccccgg
     1741 tcggctcgcc tgttgtccgg tgagcagaca accttctcca gcatccgtgc aggtgcgcgt
     1801 cggccacgcg acctcgccgc cgttctcgaa tggtcctcgt tcaccaaccc gtcgagatgc
     1861 gacggccgga aatgtcgctc accacgacac ggagcacgac gctgcggtcc tctccggccc
     1921 agggcgtggg gccgcgttcg acaagtcggc ggcgttccgt ggggtcctcg atgcgttcga
     1981 gccgaccggt caccagcacg ctccagccct cgctcatggc ctcgtcgatg tggtccactt
     2041 cgaagctgac cgggtcttgg cccgcggccg cggccaggct ggtgtcggtc gcggtgcgaa
     2101 acacgacttg gccatccacg agcaggtagt tgacgggcaa cgccaccggc ccgcgcttgg
     2161 cggcatggaa gaccagacga ccgacgcctc cggtggctaa ccggcgctgc gcctcttcgc
     2221 gagacaaggc gtcgagccct cgacgtgccg cggaccgacc gggccccggg acccggtcga
     2281 cgtcgccacc caacagcgcg cccggcgtcg tgtccagcgc cgacgcgagt cgcaacaccg
     2341 cgtccgtcgc caggtgagcc ggccgagatt cgagatcttc gaggtagccc actgccatgc
     2401 ccgtgcgcgc cgctaactct tgggggctca agcaaagctg ccgccgacga tgggcaaccc
     2461 gccgccccag gtctccgggt ccgggcatgt cgactacggc ctgactcacg atgaacgctc
     2521 gaggggcgtt ctcgaaggtg gagggctcag cggtggcgtg tgctcggcca acttggcgcc
     2581 gaggcgctgg gccacctcga ccagataccc ccgaagcgac gcggtcgcgg cctgatgtcc
     2641 ggcgaaacga tcaccgatga caccgatggc gcccagcggc ggggtgtact cgccggtgag
     2701 ccgaagctcg atcgtctcat gctcggcgtc gcggatgcga agctgcccgt tcagggtcgg
     2761 aaacagtgcc gggtgctcgg cggcctgcca ggcaaccggg acctccaggg cccggggtgg
     2821 caggccgaac ttccgggggc tgacggacat cgggccgact tcggcccg
//
LOCUS       DATCCH010000466         2245 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_371704, whole genome shotgun sequence.
ACCESSION   DATCCH010000466 DATCCH010000000
VERSION     DATCCH010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371704"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VIJ44_08980"
     CDS             complement(<1..533)
                     /locus_tag="VIJ44_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEY5166072.1"
                     /translation="MNPIGCAAVRDAAPELALGILDGAERAELLLHLAGCPRCQEHVN
                     ELAGVADGLTRLAPEVEPPFGFSRRVDAAIRGGRRRWVRRWGTVAVAAAAAAIIAVVA
                     VRVIDAGRPSPPVAVPALHTVAMIGANNVRVGRVAVSGTSPASLAVNVDYSIPDGTYT
                     LELDHPGGTDHRVGTLTV"
     gene            complement(530..1054)
                     /locus_tag="VIJ44_08985"
     CDS             complement(530..1054)
                     /locus_tag="VIJ44_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY5166073.1"
                     /translation="MAAGDADAASAFVERFQRRVFGLATTILGDHRAAEDVAQEALLR
                     AWRHAAVYDPRRGSVVTWLLTITRNLSIDAMRVRRPIAIDPDALLGLAPPAADRGPAD
                     LAVVESDVGRLRDALGTLPAEQRRAIVLAGVVGLTAREVAEREAIPLGTAKTRIRTAL
                     QRLRTALVSEDRTE"
     gene            1255..1794
                     /locus_tag="VIJ44_08990"
     CDS             1255..1794
                     /locus_tag="VIJ44_08990"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEY5166074.1"
                     /translation="MRKLLVGVTVVAATLAPTALLVPGASASVASKSKPPVKLSEKVN
                     DRGTATATGNSIELDQRDYSFTPTYVKIPAGASSIAVTVKNMGSTQHTFTVPSEQIDK
                     VLNPGDSVTVTVTIPGPGAIAFYCRFHKSLGMQGAFFDKKGAKLISKVTANGSGSSGS
                     AATTPTTKASSGSSGGYGY"
     gene            complement(1865..>2245)
                     /locus_tag="VIJ44_08995"
     CDS             complement(1865..>2245)
                     /locus_tag="VIJ44_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5166075.1"
                     /translation="RGPRVHRLVARRARRMTPTAATGPVDGCDLCEAARITPWYHEDD
                     VCWIAECEICAVPMVVWRVHDTDPPPEALGHMHDRLAAVVGEHFGFEHYVDDNMRNIP
                     DHYHAHGRPKGGFFGHGLTRPARP"
BASE COUNT          341 a          738 c          864 g          302 t
ORIGIN      
        1 acggtgaggg tcccgactcg atggtcggtg ccgccggggt ggtcgagctc gagggtgtag
       61 gtgccgtcag ggatggagta gtcgacgttg acggcgaggc tggccggtga ggtccctgac
      121 accgcgacgc gtccaacgcg gacgttgttg gcgccgatca tggcgacggt gtgcagcgcc
      181 ggtacggcga cgggaggcga cgggcgtccg gcgtcgatga cgcgcaccgc gaccacagcg
      241 atgatcgccg ccgccgcggc ggcgaccgcg acggtccccc agcgtcgcac ccagcggcgt
      301 cgaccaccac gaatggcggc gtcgacccgg cgggagaacc cgaagggcgg ctcgacctcg
      361 ggggcgaggc gggtgagacc gtcggcgacg ccggcgagct cgttgacatg ctcctggcag
      421 cggggacagc ccgcgaggtg caggagcagc tcggcgcgtt cggcgccgtc gaggatgccg
      481 agggccagct cgggagcagc gtctcgcacc gcggcgcagc cgatggggtt cattcggtcc
      541 ggtcctcgct caccagcgcg gtgcggagtc gttgcagcgc ggtgcggatg cgggtcttgg
      601 cggtcccgag cgggatggct tcgcgttcgg cgacctcgcg ggcggtgagg ccgacgacgc
      661 cggcgaggac gatggcgcgg cgctgctcgg cggggagggt tccgagcgcg tcgcggaggc
      721 gcccgacatc ggattcgacg acggcgaggt cagcggggcc gcggtcggcg gccggagggg
      781 cgaggccgag gagggcgtcg gggtcgatgg cgatcggtcg gcggacgcgc atggcgtcga
      841 tcgacaggtt gcgggtgatg gtgagcagcc aggtgacgac gctgccccgg cgggggtcgt
      901 agaccgcggc gtggcgccag gcgcgcagga gtgcctcctg ggcgacgtct tcggcggctc
      961 ggtgatcgcc gaggatcgtc gtcgccaggc cgaacacgcg acgttggaag cgctcgacga
     1021 acgccgacgc ggcgtcggcg tcgccggctg ccatccccgc caccaaagca tcgtcggcga
     1081 ctgtccacat gacaggtgat acggcggcca ccggccaccg gattgccccc accgtagatc
     1141 gcccggtgac ccccgccacc tccgggcggg tcctacgctc agggggcggg ccgccgtagc
     1201 gatcggccct aggcgcccga tcgtcgggcc cgagccacac ggggagaacg tcggatgcgc
     1261 aagctgctgg tcggagtcac ggtcgtggcg gccacgctgg cgccgaccgc gctgctcgtc
     1321 cccggggcgt cggcctcggt ggcatccaag tcgaagccgc cagtgaagct gtccgagaag
     1381 gtcaacgaca ggggcaccgc caccgcgacg ggtaacagta tcgagctcga ccagcgtgac
     1441 tactcgttca caccgacgta cgtgaagatc ccggccggag cgagctcgat cgccgtcacg
     1501 gtcaagaaca tgggttcgac ccagcacacc ttcaccgtgc ccagcgagca gatcgacaag
     1561 gtcctcaacc ctggagactc ggtgacggtg acggtcacga tcccgggtcc gggcgcgatc
     1621 gccttctact gccggttcca caagtcgctc ggcatgcaag gcgcgttctt cgacaaaaag
     1681 ggcgccaagc tgatcagcaa agtcaccgcc aacgggtccg gcagttccgg aagcgccgcg
     1741 acgaccccga cgaccaaggc gtcgagcggc tccagcggcg ggtacgggta ctgaccggcc
     1801 cggtcacctg agcggcccgg ccgaccagcc gggccggggc gtttccgatc tcgggccggt
     1861 gcggctacgg gcgtgcgggt cgggtcaggc cgtggccgaa gaagccgcct ttggggcggc
     1921 cgtgggcgtg atagtggtcc gggatgttgc gcatgttgtc gtcgacgtag tgctcgaagc
     1981 cgaagtgctc gcccacgaca gcggcgagcc ggtcgtgcat gtggccgagc gcctcaggcg
     2041 gcggatcagt gtcatggacc cgccagacga ccatcggcac cgcgcagatc tcgcactcgg
     2101 cgatccagca cacgtcgtcc tcgtggtacc agggggtgat acgcgccgct tcgcacaggt
     2161 cacagccgtc gacgggcccg gtcgccgcgg tcggggtcat gcgtcgagcg cggcgcgcaa
     2221 cgagccgatg aactcgtggg cctcg
//
LOCUS       DATCCH010000467         2347 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_371929, whole genome shotgun sequence.
ACCESSION   DATCCH010000467 DATCCH010000000
VERSION     DATCCH010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371929"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..124
                     /locus_tag="VIJ44_09000"
     CDS             29..124
                     /locus_tag="VIJ44_09000"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5166076.1"
                     /translation="MHSSGFAGLYLAHQERRIRHFHRVLEDYVGS"
     gene            121..447
                     /locus_tag="VIJ44_09005"
     CDS             121..447
                     /locus_tag="VIJ44_09005"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HEY5166077.1"
                     /translation="MTTYMPVAAESDVRPGRARSVQAGELCLLLCNVDGVVYAVADVC
                     THDRGPLGDGRLRGPAIECPRHGARFDVRDGSVLAPPAVQPLATFATRVVDGRVEVVI
                     PEEDPR"
     gene            444..1643
                     /locus_tag="VIJ44_09010"
     CDS             444..1643
                     /locus_tag="VIJ44_09010"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014213967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY5166078.1"
                     /translation="MSGPLEGIRVVELGVWVAGPAAGGILADWGAEVIKIEPHDGDPA
                     RGFLAMLGGDVPFNPPFELDNRSKRSIAVDLGASAGQTVALELIDRADVFLTNIRLDA
                     LERLGLDAASLQHRRPSLVYGIITGYGLEGPERGRAAYDIGAFWARSGIAGLLASPGG
                     EPPFQRGGMGDHGAGMALAAGICAALTARARTGDGQVVATSLLRHGMYTIGFDLNTAL
                     RFGVPIGTATHATMGNPAINCYQDRDGRWFWLIGLDGDRHWPDLCRAVGHPEWIEDLR
                     FATGRARREHLDELIPALDTIFATRTREEWGEILDREGMWWAPVQTTDEILADPQAWA
                     GGGFVDVPDGSSTQTMINSPVDFLGTPSAPRGMPPELGEHTDEVLAELGRDAATIAAL
                     RADGIVA"
     gene            1700..>2347
                     /locus_tag="VIJ44_09015"
     CDS             1700..>2347
                     /locus_tag="VIJ44_09015"
                     /inference="COORDINATES: protein motif:HMM:NF037667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family peptidase"
                     /protein_id="HEY5166079.1"
                     /translation="MIRRPTAVVLAAVVALIISAPPSAARQSSTSGIGDPYFPTLGDP
                     GYRVRHYDLALRVDPRTDTLAGTATISATAVHRLTRFRVDLAGLHVTRVAVDGKRTAV
                     VQDHDKIVISPGTALATGAGFSLTVTYGGHPSPGVVPGIGLANGWITNGSTSFTLGEP
                     DAARRWFPANDHPSEKATFTFTITVPAGLTAVANGRLVHQSQTGAGVTDKWQEDAP"
BASE COUNT          332 a          900 c          776 g          339 t
ORIGIN      
        1 cgccgcgtca cccccggtgc aggccgggat gcattcctca ggattcgccg ggctgtacct
       61 cgcgcatcaa gaacgccgca tccgccactt ccatcgcgta ctcgaggact acgtcgggtc
      121 gtgaccacgt acatgcccgt cgccgccgag agcgacgttc ggccgggtcg cgcccgctcg
      181 gtccaggccg gtgagctctg cctactgctc tgcaacgtcg acggcgtcgt ctacgccgtc
      241 gccgatgtct gcacccacga ccgcggaccg ctcggcgacg gccggctccg gggcccggcg
      301 atcgagtgtc cccgccacgg ggctcgcttc gacgtgcgcg acgggtcggt cctcgctccc
      361 ccggccgtgc agccgctggc gacgttcgcg actcgcgtcg ttgacggacg cgtcgaagtc
      421 gtgatcccgg aggaggaccc gaggtgagcg gaccactcga gggcatccga gtcgtcgagc
      481 tcggcgtctg ggtcgccggt cccgccgcgg gtgggatcct cgccgactgg ggcgcggagg
      541 tgatcaagat cgagccgcac gacggcgacc ccgcccgggg gttcctcgcc atgctgggcg
      601 gcgacgtgcc cttcaacccg ccgttcgagt tggacaaccg ctccaaacgc agcatcgcgg
      661 tcgacctggg cgcatccgcc ggtcagaccg tcgctctcga gctcatcgac cgggccgacg
      721 tgttcctcac caacatccgc ctcgacgcgc tcgagcggct cggcctcgac gccgccagcc
      781 tccagcaccg tcgcccgagc ctcgtgtacg ggatcatcac cggctatggg ctcgagggac
      841 ccgaacgggg ccgcgccgcg tacgacatcg gtgcgttctg ggcccgcagc gggatcgcgg
      901 ggctgctcgc gtcgcccggg ggggaaccgc cgttccagcg gggcgggatg ggcgaccacg
      961 gggccgggat ggcgctggcc gccgggatct gcgccgcgct caccgcccgg gcccgcaccg
     1021 gcgacggtca ggtcgtcgcg acgtcgctcc tgcggcacgg gatgtacacg atcggcttcg
     1081 acctcaacac cgcgctgcgg ttcggtgtcc cgatcggcac cgcgacccac gccacgatgg
     1141 ggaacccggc catcaactgc taccaggacc gcgacggccg ctggttctgg ctcattggcc
     1201 tcgacggtga ccgccactgg cccgacctgt gtcgcgccgt cggtcatccc gaatggatcg
     1261 aggatctccg cttcgccacc ggtcgggctc gacgcgagca cctcgatgag ctcatcccgg
     1321 ccctcgacac gatcttcgcc acccggacac gcgaagagtg gggtgagatc ctcgaccgcg
     1381 aagggatgtg gtgggcgccg gtgcagacca ccgacgagat cctcgccgac ccgcaggcct
     1441 gggccggcgg aggtttcgtc gacgtcccgg acgggtcaag cacccagacc atgatcaact
     1501 cgccagtcga tttcctcggc acccccagcg cgccgcgggg catgccaccc gagctcggcg
     1561 agcacaccga tgaggtactc gcggagctcg gccgcgacgc cgccaccatc gccgcgctcc
     1621 gagccgacgg gatcgtcgcc tgaggcgctc gttcgcgtcg cgtcccggtt ccatcgactg
     1681 atcggtaggg tcgaccagag tgatccgccg accgacggcc gtcgtcctcg ccgccgtcgt
     1741 ggcgctcatc atctcggccc ccccgagcgc ggctcgccag tccagtacct ccgggatcgg
     1801 cgacccctac ttccccaccc tcggtgaccc cggttaccgg gtccgccact acgacctcgc
     1861 gctgcgcgtt gacccgcgga ctgacaccct cgccggcacc gcgacaatca gcgccaccgc
     1921 cgtgcaccgc ctgacccgct tccgggtcga cctcgccggt ctgcacgtca ctcgcgtggc
     1981 cgttgacggc aagcgaaccg cggtggtcca ggaccacgac aagatcgtga tctcacccgg
     2041 gaccgcgctg gccaccgggg ccgggttctc actcaccgtc acctacgggg gccatccgtc
     2101 acccggggtc gtgccgggca tcggtctcgc caacggctgg atcaccaacg gttcgacctc
     2161 gttcacgctc ggcgagcccg acgcggcccg gcggtggttc cccgccaacg atcacccgag
     2221 cgagaaggcg acgttcacct tcacgatcac cgtcccggct ggcctcaccg cggtcgccaa
     2281 cgggcggctg gtccaccaga gccagaccgg cgcgggcgtc accgacaagt ggcaggagga
     2341 cgcgccg
//
LOCUS       DATCCH010000468         3166 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_34778, whole genome shotgun sequence.
ACCESSION   DATCCH010000468 DATCCH010000000
VERSION     DATCCH010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34778"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..379
                     /locus_tag="VIJ44_09020"
     CDS             95..379
                     /locus_tag="VIJ44_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020546325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEY5166080.1"
                     /translation="MREMLIQLDVDAGDRRWQERANCLGVDPDLFFPERGASTKEAKG
                     VCGGCEVRTECLEYALRHGEKFGIWGGMSERERRRIRRQRALARRGLASA"
     gene            complement(411..1304)
                     /locus_tag="VIJ44_09025"
     CDS             complement(411..1304)
                     /locus_tag="VIJ44_09025"
                     /inference="COORDINATES: protein motif:HMM:NF019454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5166081.1"
                     /translation="MLECVANVSEGRDMDVLRTIARACGPALVDVHADADHHRSVFTL
                     VGPGHRDAAGGARSLAAAVAEQCSIVGHAGEHPRFGALDVVPFVALDDTEIERAQAAA
                     EARAFAEWWAREFAVPVFLYDDADPGRRDLPSARSNCFRRRKPDFGPDTPHPRLGATA
                     VGARRRLVACNCVLPDRDVGVARRIAREIRERSGGLRGVRALGFDLAGAGHAQVSMNL
                     VELDRTGIEDACSQVRSLAQRYGTDVASVEVVGLVAQRDLDRCSDAFLRWTHLDARST
                     VEARVGNGPRWWPGDPPARLP"
     gene            1407..2414
                     /locus_tag="VIJ44_09030"
     CDS             1407..2414
                     /locus_tag="VIJ44_09030"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEY5166082.1"
                     /translation="MPSTRMQIGLALPQYDYSVAGERPLRFETIVEHARLAERGGADS
                     VWLSDHLFLDLAKYGGSSRREGCFDPIVTLAALARAVPRVRLGTLVLLEALRPAAVLA
                     KALATVDCVSGGRLDVGLGAGWYEPEYAALGRAMPRAGERLDRLADTLEVVKGLLGGG
                     PFTYEGVYHRAHAATNRPRAAQRPRPRVFVGGKGDRLLRLAAGHADGWNTCWVWTPDA
                     YRERLQVCDEACARVGRDPATMWRTLGLYALCGENERDLGRRFDRLRDRSPPGVLDGM
                     DLATFRVGRLVGTVDDVRAQLHAWEALGIDTIMLGVGAVPFQVAARDDVELLLEATTR
                     R"
     gene            complement(2433..2693)
                     /locus_tag="VIJ44_09035"
     CDS             complement(2433..2693)
                     /locus_tag="VIJ44_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEY5166083.1"
                     /translation="MDETPEEYAWMLHARCRGASPTEFFPSDGLGVESAQHICEGCPV
                     RTECLEYALANRIEHGVWGGASERERRRILRRRRELVPSPPS"
     gene            2965..>3166
                     /locus_tag="VIJ44_09040"
     CDS             2965..>3166
                     /locus_tag="VIJ44_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5166084.1"
                     /translation="MAIDPEELEEIPPQGHVRVVYTGPVAPHWEIHRVFGDQRAIDEL
                     RQRAMARLQLLPVYDPQFRRNRE"
BASE COUNT          448 a         1106 c         1149 g          463 t
ORIGIN      
        1 cgccgttgta attccatcgg cggcatttcg gcccgaccgc cccggacgcc cagccgggaa
       61 ccggtctgat cacgagcgaa cgagggggca gccgatgaga gaaatgttga tccagctcga
      121 cgtcgatgcc ggagatcggc gttggcaaga gcgagcgaac tgcctcggcg tcgatcccga
      181 tctgttcttc cccgagcggg gcgcctccac caaggaggcg aagggggtct gcgggggctg
      241 cgaggtgcgc accgagtgcc tcgagtacgc cttgcgccac ggcgagaagt tcgggatctg
      301 gggcggcatg tccgagcggg aacgccggcg catccgccgc cagcgggcgt tggcccggcg
      361 cggcctcgcc agcgcctgaa cccgcgaccc accgttccgg tgatgcgcgg ctacgggagc
      421 cgcgccggag ggtcgcccgg ccaccaacgc ggaccgttgc cgacccgggc ctcgacggtc
      481 gagcgggcgt cgaggtgggt ccaccggagg aacgcgtccg agcagcggtc gaggtcgcgc
      541 tgcgcgacga gcccgacgac ttcgacgctc gcgacatcgg tcccgtaccg ttgcgcgagg
      601 gaccgcacct gcgagcacgc atcctcgatt cccgttcggt cgagctcgac caggttcatc
      661 gacacctgag cgtgcccggc cccggccagg tcgaagccga gcgcccgcac gcctcgcagc
      721 ccgcccgacc gctcgcgtat ctcccgcgcg atccggcggg ccacgccgac gtcgcggtcc
      781 gggagcacgc aattgcacgc gacgagccgc cggcgagccc ctacggccgt cgcgccgagt
      841 cgggggtgcg gggtgtcggg tccgaagtcg ggtttgcggc ggcggaagca attcgaccgc
      901 gcgctcggga gatcgcgccg gcccggatcg gcgtcgtcgt acaagaacac cggaaccgcg
      961 aattcgcgtg cccaccactc ggcgaaggcg cgggcttccg ccgccgcctg cgcgcgctcg
     1021 atctcggtgt cgtcgagggc cacgaacggc acgacgtcca gagccccgaa gcgggggtgc
     1081 tcgccggcgt gaccgacgat cgagcactgc tcggctaccg cggcggcgag cgatcgcgcg
     1141 ccgccggcgg cgtcacggtg gccgggcccg accaaggtga agaccgatcg gtgatggtcg
     1201 gcgtcggcgt gcacgtcgac gagcgccggg ccgcacgcgc gggcgatcgt gcggagcacg
     1261 tccatgtctc gtccttcgga aacgttggcg acgcactcca gcacggcggc cagcgtacga
     1321 ctccgcgtcg agacccgaat ggagcgccgc gacgtctcga cgcgtgccgt gtgtggccgc
     1381 gccccgcacc tcggtaacct cgcccggtgc cgtcgacgcg catgcagatc gggctcgcgc
     1441 tgccgcagta cgactactcc gtggcggggg agcgacccct gcggttcgag acgatcgtcg
     1501 agcacgcgcg cctcgcggaa cggggaggtg ccgactcggt ctggctctcc gaccacctct
     1561 tcctcgacct cgcgaagtac ggcggatcct cccggcggga agggtgcttc gacccgatcg
     1621 tcacactggc cgcgctggcc cgcgcggttc cgcgcgtgcg gttggggacg ctcgtgctgc
     1681 tcgaggcact ccgaccggcc gcggtgttgg ccaaggcctt ggcgacggtc gactgtgtga
     1741 gcggcggtcg cctcgacgtg ggcctcggtg cgggttggta cgagcccgag tacgccgcac
     1801 tcgggcgggc gatgccgcgt gccggggagc gcctcgaccg gctcgccgac acgctcgagg
     1861 tcgtcaaggg tcttctcggc ggcggtccgt tcacatacga gggcgtgtac caccgcgcgc
     1921 acgcggcgac gaaccggccg cgcgccgcgc agcgtccccg cccgcgggtg ttcgtcggcg
     1981 ggaagggcga ccggttgctg cggctcgccg ccgggcacgc cgacggttgg aacacctgct
     2041 gggtgtggac tcccgacgcg taccgcgagc ggctgcaggt ctgtgacgag gcgtgcgcgc
     2101 gcgttggtcg tgacccggcg acgatgtggc gaacgctcgg gctctacgcg ctctgcggcg
     2161 agaacgagcg cgatctcggg cggcggttcg accgcttgcg cgaccggtcg ccgcccgggg
     2221 tgctcgacgg catggatctc gcgacgttcc gcgtcggccg cctcgtcggc accgtcgacg
     2281 acgtgcgggc gcagctgcac gcctgggagg cgctcggtat cgacacgatc atgctcggcg
     2341 tcggtgcggt gccgttccag gtggcggctc gcgacgacgt cgagctcctg ctcgaggcga
     2401 ccactcgccg gtgatgacgg cgcctggtcc ggtcagctcg ggggcgaagg aaccagttcg
     2461 cgacgacggc gcaggatgcg ccgccgctcg cgctcggacg ctccgcccca gacaccgtgt
     2521 tcgatgcggt tggccaacgc gtactcgagg cattccgtcc tgaccgggca cccttcacag
     2581 atgtgctggg ccgactcgac accgagaccg tcggacggga agaactcggt cgggctggcg
     2641 ccgcggcaac gcgcgtgcag catccaggcg tattcctcgg gagtctcgtc cacgatcacc
     2701 tccccttcgt tggagcctta cggctcaccg cactgcttgg ttcgacgcgg acacggggtt
     2761 tcgtcaggcg cttctccatc agaccaactg cccggatcca acgatcgaca aactctcaga
     2821 cgttcgagcg gcccgattgg cttcacgttt cgtgtgggca tttcatgaat ttccggcgcc
     2881 ggacggtgtg gcggatgggc aaaccgaccg ggtgtccgcc gggagtagcg ctaccctgac
     2941 ccgtcgtctg acgaggagcg agtgatggcc atcgaccccg aagagctcga agagatcccg
     3001 ccgcagggtc atgtgcgggt cgtgtacacc ggcccggtcg cgccccattg ggagatccac
     3061 cgggtgttcg gagaccaacg ggcgatcgac gagctccgcc agcgggcgat ggcgcggctg
     3121 cagttgctgc ccgtctacga cccgcaattc cgccgcaacc gcgagc
//
LOCUS       DATCCH010000469         4621 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_81207, whole genome shotgun sequence.
ACCESSION   DATCCH010000469 DATCCH010000000
VERSION     DATCCH010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4621
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81207"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="VIJ44_09045"
     CDS             complement(<1..789)
                     /locus_tag="VIJ44_09045"
                     /inference="COORDINATES: protein motif:HMM:NF012531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit alpha"
                     /protein_id="HEY5166085.1"
                     /translation="MPELDGCGIGFVADVTGEASRAIVELALNGLACVRHRGAVAADG
                     LSGDGAGILAPLPRAFFARVGGAALGRELDERRVGVVFAFLDLDDDVARKTAEAAVAE
                     ACTAEGLELAGWRTVPVDGSHLGPHALADLPMLTQGLLVRPEGIDDAEAERRAFRARR
                     RAEARCREEGVRHYFASWSFCTVTYKALVISDRLPLFYPDLAADDFTAGHVVFHSRFS
                     TNTLPAWERAQPFRHLCHNGEINTLQGNEHRMLARGHLGSETAGL"
     gene            1039..2148
                     /locus_tag="VIJ44_09050"
     CDS             1039..2148
                     /locus_tag="VIJ44_09050"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEY5166086.1"
                     /translation="MDETPPVIAVLGGGQLGRMLALAGLPLGLAFRFLDPSLAAPAGP
                     LGALHTGRLDDAAAVRAVAAGAEVVTYEWEGVAADAARAAEAFAPLRPGGRPLEVAQD
                     RGAEKATFERLGIHVPAFETVDDYDGLERAVAAVGLPAVLKTRRGGYDGKGQRVLHKT
                     ADVDEAWRTLGDVPLILEQLVSFRRELSIVAVRGLDGTTACYPLVENEHQDGILRVTR
                     APAPGLAPGLQREAEAIAAKLLSDFDYVGVLAVELFEHDGRLLANELAPRVHNSGHWT
                     IEGAETSQFENHLRAILGWPLGATTPRGVSAMVNCLGVLPPRDAILGVTGAHLHDYRK
                     PSRAGRKVGHITVTADSVEERDRRLAAVRGVTDGS"
     gene            complement(2161..3306)
                     /locus_tag="VIJ44_09055"
     CDS             complement(2161..3306)
                     /locus_tag="VIJ44_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEY5166087.1"
                     /translation="MAGPLAGVKIVELVGIGPGPFAAMLLADMGAEVLRVHRVQSVER
                     GFDPGGVPVLDRNRRSVGVDLKHPDGVETVLRLVAGAEALLEGFRPGVTDRLGVGPDA
                     CLARNPRLVYGRMTGWGQEGPMAHTAGHDINYIALAGALAHFGRAGDKPTPPLNMVGD
                     FGGGGMFLAFGIVCGILEARTSGQGQLVDAAMVDGTAVLMSMMWGLRQLGAFDEQHRG
                     GNVLDTGAPFYDTYECADGAFIALGSLEPQFYAELLAKTGLDDEDLPAQMDREGWPRL
                     RERFTELFKTKTRAEWCALLDASDACFAPVLTLSEAAEHPHIKARGTIVEYEGVLQPG
                     PAPRFSRTPPELARPAAKPGEHTDDALRDWGFSAAEVASLRDAGAVR"
     gene            3426..4439
                     /locus_tag="VIJ44_09060"
     CDS             3426..4439
                     /locus_tag="VIJ44_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5166088.1"
                     /translation="MRLRLDPADEYMHPLETASNFNESMYFNCFDARERVGGFLRLGN
                     RANEGYAELTTCLYLPDGRVAFTFHRPEISTNDAFDAGGMRFEVTEPFTALRAGYRGK
                     ICLLEHPLEMADPRRAFTENPWTDAEVELDYRGLSPMLGGEPVNDDGSPLTEDPTQGF
                     ARGHYEQHVGARGVIRVGDEEWQIDGYGLRDHSWGPRFWQTPWWYRWLTGNFGDDSGF
                     VLSIITARDGAQRVGGVWFADGEYRSLEHVTIDTTWRTDDQYHEHIHAVAVAGGEEHV
                     IDGTVLSLIPLRNRRRSPDGEQLITRISEGMTEWKSDGRVGYGLSEYLDQIVDGQPVG
                     VAA"
BASE COUNT          674 a         1682 c         1634 g          631 t
ORIGIN      
        1 gaggcccgcg gtctccgaac cgagatgacc gcgcgccaac atccgatgct cgttgccttg
       61 caaggtgttg atctcgccgt tgtggcagag gtgacggaag ggctgcgcgc gctcccacgc
      121 ggggagcgtg ttcgtcgaga accggctgtg gaacaccacg tgccccgcgg tgaagtcgtc
      181 ggcggcgagg tcgggataga agaggggcag ccggtcgctg atgaccaacg ccttgtacgt
      241 gacggtgcag aacgaccagc tggcgaagta gtgacggacg ccttcctcac gacagcgggc
      301 ctccgcccga cgtcgggccc gaaacgcccg acgttcggct tcggcatcgt cgatgccctc
      361 gggtcggacc agcaggcctt gggtgagcat cggcaggtca gccaaggcgt gggggccgag
      421 gtgcgagccg tcgacgggaa cggtccgcca gccggccagc tcgagcccct cggcggtgca
      481 ggcctcggcc accgccgctt cggcggtctt gcgggcgacg tcgtcgtcga ggtccaagaa
      541 cgcgaacacc actccgacgc ggcgctcatc gagctcacgg ccgagcgccg ccccgccgac
      601 gcgggcgaag aaggcccgtg ggagcggcgc gaggatgccg gccccgtcgc ccgagagacc
      661 gtcggcggcg accgcgccgc gatgacggac acaggccagc ccgttcaggg cgagctcgac
      721 gatcgcgcgc gacgcttcgc cggtgacgtc ggcgacgaag ccgatgccgc acccgtcgag
      781 ttcgggcatg cgcagacgat cttgatgcgt ggacacggtc tcctcgcggg ggtttgcaca
      841 cggttccgcg gggtcgcggg atccaagatc cggcacgccg tccacgtccc ggaaccttcc
      901 tggaaccgtg ggtcaagctg gggcgacacc ggtgtcgcga cgcgcatcgt accggacctg
      961 atcgccggcg cgggcctttt ccggcccggc ggcgctcccg tcgctcccgg acgcgcgccg
     1021 gtgcggtaga acgcgggcat ggacgagacc ccgcccgtga tcgccgtcct cggcggcggg
     1081 cagctcgggc gcatgctcgc cctcgccggg ctgcccctcg ggctcgcgtt tcggttcctc
     1141 gacccgtccc tggcggcgcc ggcgggcccg ctcggcgcgc tgcacaccgg gcggctcgac
     1201 gacgccgccg cggtgcgggc cgtcgcggcc ggcgccgagg tcgtgacgta cgagtgggaa
     1261 ggcgtcgccg ccgacgcggc acgagccgcg gaggcgttcg cccccctccg gccagggggc
     1321 cggcccctcg aggtcgccca agaccgcggc gccgagaaag ccaccttcga gcggctgggg
     1381 atccacgtcc cggcgttcga gaccgtcgac gactatgacg gcctcgagcg cgccgtggcc
     1441 gcggttggac ttcccgcggt cctcaagacc cgccgcggcg ggtacgacgg gaaaggccaa
     1501 cgggtcctcc ataagaccgc agacgtcgac gaggcctggc ggacgctcgg cgacgttccc
     1561 ctgatcctcg aacagctcgt ctcgttccga cgggagctgt ccatcgtcgc ggtgcgaggc
     1621 ctcgacggga ccaccgcgtg ctacccgctg gtcgagaacg agcatcaaga cgggatcctg
     1681 cgggtcacgc gggcgcccgc gcccgggctg gcccccgggc tgcaacgtga agcggaagcg
     1741 atcgcggcga agctcttgag cgacttcgac tacgtcggtg tcctcgcggt cgagctgttc
     1801 gagcacgacg gtcgactcct ggccaacgag ctcgcgcccc gggtccacaa ctcgggccac
     1861 tggacgatcg agggggccga gaccagccag ttcgagaacc atctgcgcgc catcctcggc
     1921 tggcccctcg gtgcgaccac gccccgcggc gtcagcgcca tggtgaactg cctcggcgtg
     1981 ctccccccac gcgacgcgat cctcggtgtc accggcgcgc acctccatga ctaccggaag
     2041 ccgagccgcg ccgggcgcaa ggtcggccac atcacggtga ccgccgacag cgtcgaggag
     2101 cgcgaccggc gactcgccgc ggtgcgcggg gtcaccgacg ggtcgtgaac gcctctcggg
     2161 tcagcggacc gcgccggcgt cacgcagcga ggcgacctcc gcggcgctga aaccccagtc
     2221 gcgtaacgcg tcgtccgtgt gctcaccggg cttcgccgcc ggccgggcca gctcgggtgg
     2281 ggtgcgcgag aaccgggggg ccggccctgg ctggagcacc ccctcgtact cgacgatggt
     2341 cccccgcgcc ttgatgtggg gatgctccgc cgcttcgctg agggtcagca ccggagcgaa
     2401 acaggcgtcg ctggcgtcca ggagcgcgca ccactccgcc cgggtcttgg tcttgaacag
     2461 ctccgtgaac cgttctcgca gccgcggcca gccctcccgg tccatctgcg ctgggaggtc
     2521 ctcgtcgtcg aggccggtct tggcgagcag ctcggcgtag aactgcggct cgagggagcc
     2581 gagcgcgatg aacgcaccgt cggcgcactc gtaggtgtca tagaacgggg cacccgtgtc
     2641 gagcacgttg ccgcctcggt gctgctcgtc gaacgcgccg agctggcgga gcccccacat
     2701 catcgacatg aggaccgccg tcccgtcgac catcgccgcg tcgaccagct ggccctgccc
     2761 cgacgtccgg gcctcgagga tcccgcacac gatgccgaag gcgaggaaca tgccaccccc
     2821 accgaagtcg ccgaccatgt tgagcggcgg ggtcggcttg tcgccggcgc gtccgaagtg
     2881 cgcgagcgcg cccgcgaggg cgatgtagtt gatgtcgtgc ccggcggtgt gcgccatcgg
     2941 gccctcttgg ccccatccgg tcatccgccc gtagaccagc cgcgggttgc gggccaggca
     3001 cgcgtcagga cccacgccga gccggtcggt gacccccggc cggaagcctt ccaggagcgc
     3061 ctccgcgccc gcgacgagcc gcaggaccgt ctcgaccccg tcggggtgct tgagatcaac
     3121 gccgaccgag cggcggttgc ggtccagcac cggcacgccc cccgggtcga agccgcgctc
     3181 gacggactga acgcgatgca cccgcaggac ctcggcgccc atatcggcga gcagcatcgc
     3241 cgcgaacggc cccgggccga tcccgacgag ctccacgatc ttcacccctg ccagcggccc
     3301 ggccatgcgt cctccctgac gtcggcgtca gactaggagg accggggctg agcccgcctc
     3361 ggccgcccga gggtggcgtc cggggctcgc ttgacccgat cgtcgacccc gggttcgata
     3421 cggggatgcg actgcgcctc gaccctgccg acgagtacat gcacccgctc gagacggcca
     3481 gcaacttcaa cgagagcatg tacttcaact gcttcgacgc ccgagaacgc gtcggtggct
     3541 tcctgcggct cggcaaccgc gccaacgagg gctacgccga gctcacgacc tgcctgtacc
     3601 tccccgacgg gcgggtggcg ttcacgttcc accggcccga gatctcgacc aacgacgcct
     3661 tcgacgccgg cggcatgcgc ttcgaggtga ccgagccctt caccgcgctc cgggccgggt
     3721 accggggcaa gatctgtctc ctcgagcacc cactggagat ggccgacccg cggcgcgcgt
     3781 tcaccgagaa cccgtggacc gacgccgaag tcgagttgga ctaccggggc ctgtctccga
     3841 tgctcggcgg ggagcccgtc aacgacgacg gcagccccct caccgaagat ccgactcagg
     3901 gcttcgcccg tggccactac gagcagcatg tcggcgcccg cggggtgatc cgcgtcggtg
     3961 acgaggagtg gcagatcgac ggctacggcc tgcgcgacca ctcctggggt ccccggttct
     4021 ggcagacacc gtggtggtac cggtggctga ccggcaactt cggtgacgac agtggcttcg
     4081 tgctctcgat catcaccgcc cgcgatggcg cccagcgcgt cggcggtgtc tggttcgccg
     4141 atggcgagta ccggtcgctc gagcacgtga cgatcgacac gacgtggcgg accgacgacc
     4201 agtaccacga gcacatccac gccgtcgccg tcgccggtgg cgaggagcac gtcatcgacg
     4261 gcacggtcct ctcgctgatc ccgctccgca accggcgccg cagtcctgac ggcgagcagc
     4321 tcatcacccg catctccgag gggatgaccg aatggaagag cgacggccgg gtcgggtacg
     4381 gcctctcgga gtacctcgac cagatcgtcg acggccagcc cgtcggcgtg gcggcctgag
     4441 cggcgccgcg tcgagcagcc gctcggcgcg agccggctcg taggctgctg cgccttggat
     4501 gacgcggcag tcgcggcggc ggtgacccgc cacctcgggg gcgacgccac cgtggaaggg
     4561 ctccggcgcc tctccggtgg cgcgtcccgg gagacctggg ctttcgacgc gcgggtgggc
     4621 g
//
LOCUS       DATCCH010000470         2323 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_35359, whole genome shotgun sequence.
ACCESSION   DATCCH010000470 DATCCH010000000
VERSION     DATCCH010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35359"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VIJ44_09065"
     CDS             <1..260
                     /locus_tag="VIJ44_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004218283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEY5166089.1"
                     /translation="HASLRDDFQVSTPELDVLVDVLVDAGAIGARLTGAGFGGCVVAA
                     TTPDRRDEILGRACDRYRARTGLEPRAFAVRAVDGAEPLQP"
     gene            complement(308..976)
                     /locus_tag="VIJ44_09070"
     CDS             complement(308..976)
                     /locus_tag="VIJ44_09070"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEY5166090.1"
                     /translation="MPFPSRLVYDGETVTLDLRPHWWYFSRHIVSGVPLLLVVILAFR
                     ISDSDIYNVVKWPVVILVLIWSLWLGLKYLQWLRTYFVVTDQRVLYRTGVLARHGVEI
                     PLDRISNINFHQGIWERIIGAGDLEVQSAGEEGDTLFENVRHPDGVQHEIYRQMEGHA
                     THASGADAIGEAVARAVRDQAGHGRAGEDVADKIEQLARLRDEGHITPDEFEQRKNRL
                     LDEL"
     gene            complement(987..2294)
                     /locus_tag="VIJ44_09075"
     CDS             complement(987..2294)
                     /locus_tag="VIJ44_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HEY5166091.1"
                     /translation="MRDRHRWRNWGGNQRCTPAAIEAPRSDLEVTEAVRRARHADQTI
                     KVVGTGHSFTDIACTTGRMMSIDDLDRVVDVDLDTSCVTVEAGITIHRLNEELAVRGL
                     AFANLGDIDRQTIAGAISTATHGTGATFGGLATFVRALELVTADGDVLRCSADEEPEV
                     FHAARVGVGALGVVTKVTLQCVPAFNLHHLEQRRHLDGVLEELDDLVDANEHFEFYWM
                     PHTDSCATIANNRTEDPPVVKSAYKAWRAEVFYPNYFFGTIVALGRFVPSLVPRLNAV
                     AAGGVGTTELTNRSDRILVSTRLVRFVEMEYAIPRERARDAVHGVQAVIERNDLRIGF
                     PIEVRFTAPDDIPLSTAHGRDTCYVAVHVPRGIDFEPYFRGVEALMNELDGRPHWGKM
                     HYQTAETLASRYPQWDRFQAVRRKLDPEGRFANAYLDRVLGPA"
BASE COUNT          355 a          745 c          843 g          380 t
ORIGIN      
        1 gccacgccag cctccgcgac gactttcagg tctcgacccc cgaactcgac gtgctcgtcg
       61 acgtgctcgt cgacgcgggc gcgatcggcg cccggctgac cggtgccggc ttcggcggct
      121 gcgtcgtggc cgcgaccacc cccgaccgtc gtgacgagat cctgggccgc gcctgcgatc
      181 ggtatcgggc gcgcaccggg ctggagccgc gcgccttcgc ggtccgggcc gtcgatggcg
      241 ccgagccgct ccagccctga tcggcgcggg cagcaccccg cgtggagcgg agtgctcgga
      301 gtgggtgtta cagctcgtcg aggagccggt tcttgcgttg ctcgaactcg tcgggggtga
      361 tgtggccctc gtcgcggagg cgggcgagct gttcgatctt gtcggcgacg tcttcgccgg
      421 cccgaccgtg gccagcctgg tcgcgcaccg cacgggcgac ggcctcgccg atggcgtcgg
      481 cgccgctggc gtgggtcgcg tggccctcca tctggcggta gatctcgtgc tggacgccgt
      541 cggggtggcg gacgttctcg aagagggtgt cgccctcttc gcccgcggac tgcacttcga
      601 ggtcgccggc gccgatgatc cgctcccaga tcccttgatg gaagttgatg ttgctgatgc
      661 ggtcgagggg gatctcgacg ccgtgacggg cgaggacccc ggtccggtac aggacccgct
      721 ggtcggtgac gacgaagtag gtgcgcagcc actggaggta cttgagtccc agccagaggc
      781 tccagatcag cacgaggatg acgacgggcc atttcacgac gttgtagatg tcggaatcgg
      841 agatccggaa cgccaagatg acgaccagca acagcggcac cccactcacg atgtggcgcg
      901 agaagtacca ccagtgcggg cgcagatcga gcgtgaccgt ctcgccgtcg tagacgagcc
      961 gactcggaaa cggcatgacg cgcaggctac gcggggccga gcacccgatc gaggtaggcg
     1021 ttcgcgaacc gaccttcggg gtcgagcttg cgacgcacgg cctgaaatcg atcccattgc
     1081 gggtaccgag acgcgagtgt ctccgcggtt tggtagtgca tctttcccca gtggggccgc
     1141 ccgtcgagct cgttcatgag cgcttcgaca ccgcggaagt acggctcgaa gtcgatcccg
     1201 cgagggacgt gcaccgcgac gtaacacgtg tcgcgcccgt gggcggtgga gagcgggatg
     1261 tcgtcgggcg cggtgaaccg cacctcgatc gggaacccga tccgaagatc gttgcgttcg
     1321 atgacggctt ggaccccatg gacggcgtcg cgggcgcgct cgcgcgggat ggcgtattcc
     1381 atctcgacga aacggaccag ccgggtgctg accaggatcc ggtcgctgcg gttggtgagc
     1441 tcggtggtcc cgacgccgcc ggcggcgacc gcgttgagac gcgggaccag tgagggcacg
     1501 aaccgcccga gcgcgacgat cgtgccgaag aagtagttgg gatagaacac ctcggcccgc
     1561 cacgccttgt aggcgctctt gaccacgggc gggtcctcgg tgcggttgtt cgcgatcgtg
     1621 gcacacgaat cggtgtgcgg catccagtag aactcgaaat gctcgttggc gtcgacgagg
     1681 tcgtcgagct cctcgaggac accatcgagg tggcggcgtt gctcgagatg atgaaggttg
     1741 aacgcaggca cgcactgcaa cgtcaccttg gtgacgacgc cgagcgcccc gacgccgacc
     1801 cgggcggcgt ggaacacctc gggctcctcg tcagcggagc agcgcaacac gtcgccgtcg
     1861 gcggtcacga gctccagggc ccgcacgaag gtcgccaatc ccccgaacgt cgcgccggtg
     1921 ccgtgggtgg cagtcgagat ggctccggcg atcgtctggc ggtcgatgtc accgaggttg
     1981 gcgaacgcca ggccccgaac cgcgagttcc tcgttgaggc ggtggatggt gatcccggcc
     2041 tcgacggtga cacacgacgt gtccaggtcc acgtcgacga cccggtcgag gtcgtcgatc
     2101 gacatcatcc gaccggttgt gcacgcgatg tcggtgaacg agtggccggt gccgaccacc
     2161 ttgatcgtct ggtcggcgtg gcgggcccgc cggacagctt cggtgacctc gaggtcgctg
     2221 cgcggcgctt cgatcgccgc gggggtgcag cgctggttcc caccccagtt tcgccagcgg
     2281 tgacggtcgc gcatcggcgc agatcgtatg gggatggcgg gcg
//
LOCUS       DATCCH010000471         4851 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_35793, whole genome shotgun sequence.
ACCESSION   DATCCH010000471 DATCCH010000000
VERSION     DATCCH010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4851
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35793"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VIJ44_09080"
     CDS             complement(<1..443)
                     /locus_tag="VIJ44_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015888876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5166092.1"
                     /translation="MGVFDGKVAVVTGAGRGIGRAEAQLLAEHGAAVVVNDLGGERTG
                     EGTDQRPAQLVVDEIVAAGGRGAANYDDISTWEGGAALINQAVEEFGGLDVVINNAGI
                     LRDKMSFNMTEEEWDAVIRVHLKGHFVPARHAAAYWRAKSKETGEP"
     gene            828..1085
                     /locus_tag="VIJ44_09085"
     CDS             828..1085
                     /locus_tag="VIJ44_09085"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY5166093.1"
                     /translation="MTHPDKGTEMFLYELSVAESFRGRGTAAAPVTALRDLARQRGCY
                     DMWVLTDADNAAALATYQSTGANVSTSHGMLSWDFDPPDSH"
     gene            complement(1110..1904)
                     /locus_tag="VIJ44_09090"
     CDS             complement(1110..1904)
                     /locus_tag="VIJ44_09090"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY5166094.1"
                     /translation="MIHYDVGDDHVARITLDRPDAKNALTIEMRDGIVDAIRDARANG
                     DVRAVLITGTGDAFCAGMDLGASTMAQAGSADFETRSTSEALRIGVQSFIRELWELDK
                     PTVAAVNGTAVGPGAHLALACDFVIVHDDTRFLWSFARWGLVVDAGGAYLLPRLVGLP
                     RAKAMVMLGDGARGAEAVDLGLAYRCVSRDELTSVADTLAARLAAGPTRSLGLSKRLL
                     NASFESSLDRSLELEGAYQSLAATSRDLVEGMAAFKERRDPGFTGR"
     gene            complement(1901..3079)
                     /locus_tag="VIJ44_09095"
     CDS             complement(1901..3079)
                     /locus_tag="VIJ44_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5166095.1"
                     /translation="MSWNRVAVVGAGLIKFGELFEQSYEQMAAGAFAAAVEAVDKGFE
                     PAQVEAAFVATQRGTLWGQEGIGGNTVPSAIGLAGIPCTRIENACPSGSDAFRVGAMA
                     VASGVHDVVLVIGVEKMRDKSSEEGLLARAAAGHPIFTRGETAPVLFAPFAARHMHEF
                     GTTREMLASVAVKNHYNGARDPYAHFQNELTIDDVLASAPVCHPLHLLDCCPQTDGAA
                     ATLLVRADLADRFTDHPVYVAGFGVATDHPYLHEKSSFVGLKATQLAAERAYAMADLG
                     PSDIDCAEVHDCFTITEILDIEDLGFVDKGKGGVASLAGETALTGRIPVNTSGGLLAK
                     GHPIGATGVAQLTECWWQLRGEAGDRQVETHHGNALQHNVGGRGSGVSVINILTTNYP
                     "
     gene            complement(3076..4485)
                     /locus_tag="VIJ44_09100"
     CDS             complement(3076..4485)
                     /locus_tag="VIJ44_09100"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEY5166096.1"
                     /translation="MTPAVQLLAVGAAAPAWRLAAPEVGAAWGRSASASQSAVCAPDE
                     DVLTLAAEAASQALAASGLQTDIVDGLWWGTTRPPFAEGPSHAVLARALGLSHRSGGA
                     LCSGSPHSGIEALLGAADAVGAGSARVALVVVSDALVPGLGTPFEARSGAGAAALLLV
                     SHGGHASLGPRVTRTHPYLDRYRGDGELATRDLYDGRLFREEIFLPTMRELGEQLGAL
                     NISRWSLPDPDGRLGATIARQLGADSPASAPVYRALGDTGAAAALLGAIGALDTAGTV
                     GVVAWGGGRATGVTFTAREPVRGAATVAGAIARGRAASYAEVLRARRQLVPSGETVQM
                     GVPPESAQFVRGADEMLGLLGARCVDCGTINTPPSIHPHCISCGSAKFVLEPLARHGQ
                     VHTFVVNHTMPAPFVAPLPIAVIDLDDGARVMLQVTEHEAPLEIGAEVELVLRRYAHE
                     RGVPVYGYKARTIGVEGTT"
     gene            4585..4659
                     /locus_tag="VIJ44_09105"
     tRNA            4585..4659
                     /locus_tag="VIJ44_09105"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4618..4620,aa:Cys,seq:gca)
BASE COUNT          689 a         1693 c         1741 g          728 t
ORIGIN      
        1 ggctcaccgg tctctttgct cttcgcccgc cagtacgcgg cggcgtgacg cgcgggcacg
       61 aaatgacctt tgaggtggac gcggatcacc gcgtcccatt cctcttcggt catgttgaag
      121 ctcatcttgt ctcggaggat cccggcgttg ttgatgacga cgtcgaggcc gccgaactcc
      181 tcgacggcct ggttgatcag cgcggcgccg ccctcccagg tggagatgtc gtcgtagttg
      241 gcggcgcccc gaccgccggc ggcgacgatc tcgtcgacca ccagctgggc gggccgttgg
      301 tcggtgccct caccggtgcg ttccccgccg aggtcgttca cgaccacggc ggctccgtgt
      361 tcggcaagca gctgcgcttc ggcgcggccg atgccccgcc ccgcgccggt cacgaccgca
      421 accttcccgt caaagacacc catcacgctc tccatcccgg ttcacttcgc acgagtcgcc
      481 cgcctggcga tcgcggccct gggcgcgcca cccggggttc aagccgggtc ggagggcgcc
      541 gttccgcctg ctccggccgc gggtgcgtgc cgggcgacct gacgtgggcg tcaggatggt
      601 accggaccga cggggacgac ccgcctcccg gcggtggctg ttcggtggtc cctcacccag
      661 atcgcggaac tgctggactg gccctcgtga gattcgacgc atcgcgtcag cgctcgttga
      721 cggcccggcg aagcccgaat tcgcccaacg gttccttcca tccgttggcc gtttcctctt
      781 cgtggcctac gacggaggaa cccatcggat ctgtcagcgg ggtcgagatg acccaccctg
      841 acaaaggcac ggagatgttc ctctatgaac tctccgtcgc ggaatcgttt cggggtcgag
      901 gaaccgccgc cgcgcccgtc accgcgctcc gcgatctcgc ccgccaacgg ggctgctacg
      961 acatgtgggt cctgaccgac gcggacaacg cggccgccct cgcgacctac cagtcgaccg
     1021 gtgccaacgt ctcgacgagt cacgggatgc tctcatggga tttcgacccg ccagattcac
     1081 actgagcccc gcccgccccc gggattcggt cagcgacccg tgaatccggg gtcgcggcgt
     1141 tccttgaacg cagccatccc ttcgacgagg tcacgcgagg tcgcagcgag ggactggtag
     1201 gccccctcga gctcgagcga ccggtccagg gagctctcga agctcgcgtt gagcagccgt
     1261 ttcgagaggc cgagggagcg ggtcggaccg gcggcgaggc gcgccgcgag cgtgtccgcc
     1321 accgatgtga gctcgtcgcg gctcacgcag cggtaggcga gaccgaggtc gacggcttcc
     1381 gcgccgcggg cgccgtcccc gagcatgacc atggccttgg cgcgggggag gcccaccagg
     1441 cgggggagga ggtaggcgcc gccggcgtcg acgacgagac cccaccgggc gaaggaccac
     1501 aggaatcggg tgtcgtcgtg gacgatcacg aagtcgcagg ccaacgcgag gtgcgcgccg
     1561 gggccgacgg cagtgccgtt caccgccgcc accgtgggct tgtcgagctc ccacagctca
     1621 cggatgaagc tctgcacccc gatacgcagc gcctcggagg tgctgcgggt ttcgaagtcg
     1681 gccgatccgg cctgggccat tgtggacgcc ccgagatcca tgccggcgca gaaggcgtcc
     1741 ccggtcccgg tgatcagcac ggcgcggacg tcgccgttcg cgcgggcgtc gcggatcgcg
     1801 tccacgattc cgtcccgcat ctcgatggtg agtgcgtttt tggcgtcggg acggtcgagc
     1861 gtgattcgcg cgacgtggtc gtcacccacg tcgtagtgga tcacgggtag ttcgtggtga
     1921 ggatgttgat gaccgacacg ccggagccgc gaccgccgac attgtgttgg agcgcgttgc
     1981 cgtggtgggt ttcgacctgg cggtcaccgg cctcgccgcg aagctgccac cagcattccg
     2041 tgagctgcgc gacaccggtc gcgccgatcg gatgaccctt ggcgaggagg ccgccggagg
     2101 tgttcaccgg gatgcgtccg gtgagcgcgg tctcgccggc gagcgacgcg acgcccccct
     2161 tccccttgtc gacgaagccg aggtcttcga tgtcgaggat ctcggtgatg gtgaagcagt
     2221 cgtggacctc ggcgcagtcg atgtcggaag gaccgagatc cgccatcgcg tacgcgcgtt
     2281 ccgcggcgag ctgggtggct ttcagtccga cgaagctcga tttctcgtgc aggtagggat
     2341 ggtcggtggc gactccgaac ccggcgacgt acaccgggtg gtcggtgaac cggtccgcga
     2401 ggtcggcgcg caccaggagg gtggcggcgg ctccgtcggt ctgggggcag cagtcgagga
     2461 gatggagcgg gtgacagacg ggagctgacg cgaggacgtc gtcgatggtg agctcgttct
     2521 ggaaatgggc gtacggatcg cgggcgccgt tgtagtggtt cttgaccgcg acggacgcca
     2581 gcatctcgcg ggtggtgccg aactcgtgca tgtgacgggc tgcgaacggt gcgaacagca
     2641 ccggggcggt ctcgcctcga gtgaagatcg gatggccggc cgcggcgcgg gcgagcagcc
     2701 cttcctcgct cgacttgtcg cgcatcttct cgacgccgat caccaggacg acgtcgtgga
     2761 cgccgctggc gaccgccatc gcgccgaccc gaaaggcgtc ggagccggac gggcaggcgt
     2821 tctcgatccg agtgcagggg atcccagcca gcccgatggc cgacgggacc gtgttgccgc
     2881 cgatgccttc ctggccccac agcgttcccc gttgggtggc gacgaacgcc gcttcgacct
     2941 gggcgggttc gaaacccttg tcgacggctt cgaccgcggc cgcgaacgct ccggcggcca
     3001 tctgctcgta gctctgctcg aacagctccc cgaacttgat cagtccggcg ccgacgaccg
     3061 cgactcgatt ccaactcacg tggtgccctc caccccgatg gtccgggcct tgtagccgta
     3121 gaccggcacg cctcgctcgt gggcgtaccg gcgcaggacg agctcgactt cggcgccgat
     3181 ctcgagtggc gcctcgtgct ccgtgacctg gagcatgaca cgcgcgccgt cgtcgagatc
     3241 aatgaccgcg atcggcagcg gcgcgacgaa cggtgccggc atggtgtggt tcacgacgaa
     3301 cgtgtggacc tggccgtggc gggccagcgg ttcgagcacg aacttggcgc tcccacagct
     3361 gatgcaatgc gggtggatcg acggcggggt gttgatcgtg ccgcagtcaa cgcagcgggc
     3421 cccgaggagg cccaacatct cgtcggcgcc gcgcacgaat tgggcgctct ccgggggcac
     3481 gcccatctgc accgtctcgc ccgacgggac cagctgacgc cgggctcgga gcacctcggc
     3541 gtagctcgcg gcgcggcccc gcgcgatcgc gccggccacg gtcgcggcgc cgcggacggg
     3601 ttcccgcgcg gtgaaggtga ccccagtggc ccggccgcca ccccacgcga ccacgccgac
     3661 ggtcccggcg gtgtcgagcg ccccgatcgc gccgaggagc gccgcggcag ccccagtgtc
     3721 accgagcgcc cggtagaccg gcgccgacgc gggcgagtcc gcgccgagct ggcgggcgat
     3781 ggtcgcaccg agacgaccgt cggggtccgg gagggaccac cggctgatgt tgagtgcgcc
     3841 gagctgttcg cccagctcgc gcatcgtggg gaggaagatc tcttcccgga agaggcgccc
     3901 gtcgtacagg tcgcgggtcg cgagctcgcc gtcgccgcgg taccggtcga ggtacgggtg
     3961 ggtccgggtg actcggggcc cgagggacgc gtggccgcca tgcgagacga gcagcagcgc
     4021 ggcggcgccg gcacccgagc gagcctcgaa cggcgttccc agcccgggca cgagcgcgtc
     4081 ggacaccacg acgagggcga cgcgcgcgct cccggcaccc accgcatcgg ccgcgccgag
     4141 cagcgcttcg atcccggagt ggggagagcc gctgcacaac gcgccgccgg accggtgcga
     4201 aaggccgagc gcccgggcga gcacggcgtg gctcgggcct tcggcgaacg gcggccgggt
     4261 cgtgccccac cagaggccgt cgacgatgtc ggtttggaga cccgaggcgg cgagcgcctg
     4321 gctggccgcc tccgcggcga gcgtgaggac gtcctcgtcc ggggcgcaga ccgccgactg
     4381 gctggccgac gccgagcggc cccacgcggc cccaacctcg ggtgccgcga gccgccaggc
     4441 cggtgcggcc gcaccgacgg cgaggagctg cacggccggc gtcacgaccc cgatgctacg
     4501 cgggtgagcg gccgccacct tcggcgaagg cggcccggat cggcccggta gcctccactc
     4561 ttctgccgcc gacctggcgg ccccggcggc gtagccgagt ggcccaggca agggtctgca
     4621 aaaccctgga cgtgggttca attcccaccg ccgcctccag accgttcgcc ctgttcaagc
     4681 ggagttttcg gctgccggac gagcccaacg cgaccgcttg cagcccccgg acggcccgat
     4741 tgctcgttcg cccgttcggc gattctgact gggctgttcc gtaccgcccc ccgctccgcg
     4801 cgaccagttc tgagttggga gaggccggtg ccacacggac ccccgtcgtc c
//
LOCUS       DATCCH010000472         2713 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_82404, whole genome shotgun sequence.
ACCESSION   DATCCH010000472 DATCCH010000000
VERSION     DATCCH010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82404"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..664)
                     /locus_tag="VIJ44_09110"
     CDS             complement(17..664)
                     /locus_tag="VIJ44_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase"
                     /protein_id="HEY5166097.1"
                     /translation="MGPDRSRELRAAGHAASTELEGRHHAREVTLPLCRRAIRASALA
                     IRAVHRGALEDGRARITEVADLLAQAAAATEQHPGIRDAGYVHDAQKEYAEACLTLAY
                     VAHETAPDAATLGVDIPAYLNGMAEAASELRRQVLDRLRDGDTTEAQTLFAVMDDVYD
                     LLVTVDYPDALTGGLRRSTDALRAVLERTRGDITNAIVLARFDQTVSGDQPAASS"
     gene            complement(673..1242)
                     /locus_tag="VIJ44_09115"
     CDS             complement(673..1242)
                     /locus_tag="VIJ44_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5166098.1"
                     /translation="MAVVVILVLGILWAAVLVPPILRARAEGGGSGVSGGVGEVMSMF
                     TSAFGRARGTGGDQDLPAMQPLMGPVGPVAAMGAMHTPGAMSSAQKRRRDVLIGLLVA
                     VGITLVMALFSGANIMFLVLNLLADACLGGYVYLLLQMKSRQRGRRPQARPLEPTGRH
                     LRSIPGMAAEPASASGAEPALLLRRTATY"
     gene            complement(1371..1871)
                     /gene="moaC"
                     /locus_tag="VIJ44_09120"
     CDS             complement(1371..1871)
                     /gene="moaC"
                     /locus_tag="VIJ44_09120"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEY5166099.1"
                     /translation="MTASEFTHLDPLGRARMVDVTAKEPSHRRAVARCRIYMAPATAG
                     KIASGAVTKGDVLAVARVAGIQAAKQTPSLIPLCHPLLVGSVFVNFRIEEDHVEVECQ
                     VDTIDRTGVEMEALTAAAVSALTIYDMCKSLDRGMIIGELALWEKTGGRSGHWRRADD
                     HQVGDG"
     gene            complement(1985..>2713)
                     /locus_tag="VIJ44_09125"
     CDS             complement(1985..>2713)
                     /locus_tag="VIJ44_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY5166100.1"
                     /translation="DLAMTTNGVRLPALAHDLAAAGLRRVNVSLDSLRPETFRALTRR
                     DELDQVLAGIDAALDAGLDPVKVNCVVIRGVNDDEVVDLAAYGRARGVGIRFIEFMPL
                     DAPGEWSMDQVVPAAEILDRIDRVFPLADRGGPVDHVEPAAVHRYADHVGDVGVIASV
                     TEPFCGDCDRVRITAEGRFRTCLFALDETDLRAILRSGRPAEDIDTELAAAISGAVSA
                     KWAGHRIGQVNFVRPARSMSQIGG"
BASE COUNT          402 a          877 c         1038 g          396 t
ORIGIN      
        1 gaggcgggtc gcggcgtcag gacgacgctg ccggttggtc gccactcacg gtctggtcga
       61 agcgggcgag gacgatggcg ttggtgatgt cgccgcgtgt gcgttcgagg acggcgcgga
      121 gcgcgtcggt tgagcgtcgg agccccccgg tgagcgcgtc gggatagtcc acggtgacga
      181 gcaggtcata gacgtcgtcc atgaccgcga acaaggtctg ggcttcggtg gtgtcgccgt
      241 cacggagacg gtcgagcact tggcggcgga gctcgctggc ggcttcggcc atcccgttga
      301 ggtaggcggg gatgtcgacg ccgagggtgg cggcgtcggg ggcggtctcg tgcgcgacgt
      361 aggcgagcgt cagacaggct tcggcatatt ccttctgggc gtcgtggacg tagccggcgt
      421 cgcggatccc agggtgctgt tccgtggcgg ccgcggcctg ggcgaggaga tcggcaactt
      481 cggtgatccg ggcccgcccg tcctcgaggg cgccgcggtg cacggcgcgg atcgccaggg
      541 cactggcgcg aatcgctcgt cgacacagcg gcaaggtgac ctcgcgcgcg tgatgacggc
      601 cttcgagttc ggtgctggcg gcgtggcccg ctgcgcgcag ctcgcgcgag cggtccggcc
      661 ccaccgggcg gctcagtacg tcgcggtgcg gcgcaggagc agcgccggct cagccccgga
      721 ggcggaggcg ggttcggccg ccatgcccgg gatcgagcgg aggtgacgac cggtgggctc
      781 gaggggtcgc gcctggggcc gccgtccgcg ttggcgggac ttcatctgga gcagcaggta
      841 gacgtacccg ccgaggcagg catcggcgag gaggttcaac accaagaaca tgatgttggc
      901 gccgctgaac aaggccatca cgagcgtgat gccgacggcg acgaggagac cgatgagcac
      961 gtcccggcgt cgcttctggg ctgagctcat cgcgcccggg gtgtgcatcg cacccatcgc
     1021 cgcgaccggg ccgacgggac ccatgagcgg ctgcatcgcg ggaaggtcct gatcgccgcc
     1081 ggtgccgcgg gcccgtccga acgccgacgt gaacatcgac atgacctcgc cgacgccgcc
     1141 ggagacacct gatccgccac cctccgcgcg tgctcggagg atcgggggga ccagtactgc
     1201 agcccacaag attccgagga ccaggatgac gacgacggcc acggtgcggt aggtgctcct
     1261 gtgctcagtc cggttcgggc acgacgacgt ccaatggatc cgccgtggga atactgagaa
     1321 ggttagtcag accgggcccg gcggagtcaa tccgcagcgg cgagaaagct tcacccgtcg
     1381 ccaacctggt ggtcgtcggc gcgccgccag tgaccagacc tgccgccggt cttttcccat
     1441 aaggccagtt cgccgatgat catgcctcga tcgagcgatt tgcacatgtc gtagatggtc
     1501 agggccgaga ccgcagcggc ggtgagggcc tccatctcga cgccggtgcg gtcgatggtg
     1561 tcgacctggc actcgacctc gacgtggtcc tcctcgattc ggaagttcac gaacaccgac
     1621 ccgaccagga gggggtgaca cagcgggatg aggctcggcg tctgcttggc ggcctggatc
     1681 ccggcgaccc tggccaccgc gaggacgtca cccttggtga cggccccgga ggcgatcttg
     1741 ccggccgtgg cgggcgccat gtagatccgg cagcgggcga cggcccgccg gtggctgggc
     1801 tccttcgccg tgacgtcgac catccgggcc cgcccgagag ggtcgaggtg ggtgaactcg
     1861 gacgcggtca cgcgccctca gtgtatgggg cgaccgaccg gcccggtcgt ttcggggcga
     1921 gctcgatgct cggacggtgc cgcggaccaa ccggaagggg gtggcggcgg gccgaccgga
     1981 ggcctcagcc gccgatctgg ctcatggacc gcgccgggcg tacgaagttg acctgaccga
     2041 tccgatggcc ggcccacttg gcgctcactg cgcccgagat cgccgcggcg agctcggtgt
     2101 cgatgtcctc ggcaggacga cccgatcgca ggatggcccg gaggtcggtc tcgtcgagcg
     2161 cgaacaggca ggtccggaag cgcccctcgg cggtgatccg cacccggtcg cagtctccgc
     2221 agaacggctc ggtgacgctg gcgatgacgc cgacgtcgcc gacgtgatcg gcgtagcggt
     2281 gcaccgcggc cggttcgacg tggtcgaccg gtccgccgcg atcggcgagc gggaagaccc
     2341 gatcgatgcg atcgaggatc tcggcggccg ggacgacctg gtccatcgac cactcaccgg
     2401 gcgcgtcgag cggcatgaac tcgatgaagc ggatcccgac gccacgggcc cgaccgtacg
     2461 cggcgaggtc gacgacctcg tcgtcgttga cgccgcggat gacgacgcag ttcaccttga
     2521 ccgggtcgag gccggcgtcg agcgcggcgt cgatgccggc gaggacctga tcgagctcgt
     2581 cgcgtcgggt cagggcgcgg aaggtctcgg ggcgcagcga gtcgagcgag acgttcacgc
     2641 ggcgtagccc ggccgcggcg aggtcgtggg cgagcgcggg gagccgcacc ccgttcgtcg
     2701 tcatcgccag gtc
//
LOCUS       DATCCH010000473         3671 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_160483, whole genome shotgun sequence.
ACCESSION   DATCCH010000473 DATCCH010000000
VERSION     DATCCH010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3671
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160483"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1300
                     /locus_tag="VIJ44_09130"
     CDS             <1..1300
                     /locus_tag="VIJ44_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5166101.1"
                     /translation="RHSVPARITLDAGGGQTRVVDVPPVGSASVENGTVAVPVTFPAV
                     TTDHVRVTIDAVRPVNTIEYYSNLPTNLPVAIAELGIPGVQRPAPAATVPNACRSDLV
                     TLDGQPLPVRISGNTTAAVSGAALTVEACDPTGGTAAPIHLRAGVHTVRAVPGTQTGL
                     NLDSLVLASNSGGGAVALQGPVDTLATTAAPAPRIDVTSNGETTIRARVHGATGPFWL
                     VLGQSFNQGWQATVNGHDLGRPQLVDGMSNGWHVDPGNNHDFTLTLTWTPQSRIWLAL
                     AISGLAMLLCALLALFARRGLATAGESTDQPVELASPFLAGGVTPSRRAVISTTGAIT
                     VAGALFVTPWVGLVAGAATLAVLFRPRLRWLLAVGAPAALAVAGGYVFIQQWRYMYPA
                     VFEWPTFFDGVHVVGWLAVVLLTADAVVEVVRSKPRPDTE"
     gene            1419..2141
                     /locus_tag="VIJ44_09135"
     CDS             1419..2141
                     /locus_tag="VIJ44_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY5166102.1"
                     /translation="MSTTEVPGSTSRYRKLSVIVPVYNERNTVAEIVRQMRAVELPVA
                     REIVIVDDGSDDGTRDVLSQLADSTVHVVLHPHNRGKGAAIRTGLAQITGDLVLIQDA
                     DLEYDPEDWPKLISPVLKGRARVVYGSRFTGERRNMLFLHWVGNRFLSLLTNVLYNTT
                     LSDMETCYKLFDRELIDAMDLRSDGFDFEPEVTAKILRRGIRIYEVPISYSGREFDEG
                     KKITWRDGLVALKTLVKYRFIN"
     gene            2355..>3671
                     /locus_tag="VIJ44_09140"
     CDS             2355..>3671
                     /locus_tag="VIJ44_09140"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEY5166103.1"
                     /translation="MAETKGLAYQPALDGLRALAVGSVIAYHLDAGVAHGGFLGVDTF
                     FVLSGFLITSLLIGEWGQSRTINFAKFWLRRARRLLPALLLVLFAIAVYAAVSAPATQ
                     LNQLRGDGLATLFYGANWRFIASGQSYFTLFTTASPLRHMWSLAIEEQFYLVWPLITF
                     ACLWLARGRRWLLATVCVVGTAASALTMASLYQRADPSRAYYGTDSRAQLLLVGCLLA
                     LVLARWSPTTTRARTAVSVAGAVGVAYCFFAWTQITDTASWMYRGGYLLFGLAVAAVI
                     TSAVQPGRFPVRAALSVGPLVWVGRISYGLYLWHWPVIVICSPDRVGLDGWQLAVVRL
                     ALTFGIATLSYYLVELPIREGRLFRRRFGLAIPTAFVGAGVAVMVATAGAAPLPTYLQ
                     GATHVVKAAGESTKAPTPTPGVATPGLPAAPPRRLLLVGDSLAVSLL"
BASE COUNT          498 a         1405 c         1133 g          635 t
ORIGIN      
        1 ccgccactcc gtcccggctc gaatcactct cgatgccggc ggcggtcaga cccgcgtcgt
       61 cgacgtccca ccggtcggca gcgcctcggt cgagaacggc accgtcgccg tgccggttac
      121 cttccccgcc gtgaccaccg accacgtccg cgtcacgatc gacgccgtcc gcccggtgaa
      181 cacgatcgag tactactcca acctccccac caacttgccc gtcgccatcg ccgagctcgg
      241 catccccggc gtgcaacggc ccgccccagc cgcaaccgtg cccaacgcat gccgttccga
      301 cctcgtgacc ctcgacggcc agccgctccc ggtccgcatc agcgggaaca ccaccgctgc
      361 ggtcagcggc gccgcgttga cggtcgaggc ctgcgacccg accggtggca ccgccgcacc
      421 gatccatctc cgcgccggcg tgcacacggt gcgagccgtc cccgggaccc agaccggact
      481 caacctcgac agtctcgttc ttgcctccaa ctcgggtggg ggagcggtcg cgctacaagg
      541 cccggtcgac accctcgcga ccaccgccgc tcccgcgccg cggatcgacg tcaccagcaa
      601 cggcgagacc accatccgag cccgcgtgca cggcgccacc ggacccttct ggctcgtgct
      661 cggacagagc ttcaaccagg gctggcaagc caccgtcaac ggccacgacc tcgggcgacc
      721 ccagctcgtc gacggaatgt ccaacgggtg gcacgtcgac cccggcaaca accacgactt
      781 caccctgacc ctcacctgga ccccgcagag ccgcatctgg ctcgccctcg cgatctctgg
      841 tctcgccatg ctcctctgcg cgctcctcgc cctcttcgcc cgacgcgggc tcgccaccgc
      901 tggtgaatcg accgaccagc cggtggagct cgcgtcaccg ttcctcgccg ggggcgtcac
      961 cccctcgcgt cgcgcggtga tctccaccac gggcgcgatc accgtcgcgg gtgccttgtt
     1021 cgtcacgccg tgggtcggtc tcgtggccgg cgccgccacc ctggcggtcc tgttccggcc
     1081 ccgcctgcgt tggctacttg ccgtcggcgc gcccgccgcc ctcgccgtcg ccggcgggta
     1141 cgtgttcatc cagcagtggc ggtacatgta cccggcggtc ttcgagtggc ccacgttctt
     1201 cgacggcgtc cacgtcgtcg ggtggctggc tgtcgtcctg ctcaccgccg acgcagtggt
     1261 tgaggtcgtc cgctccaagc cgcgacccga cacggagtag ctcacctcga tgtggcattg
     1321 gtcccgaccg gcccgccgcc ggccaccccg ggtggggtcg caccgtgtgg cggcgctgcg
     1381 cgacactgac gactcgactg actcgtaccg gagggtcggt gtcgaccact gaagtccccg
     1441 ggtccacctc ccggtaccgc aagctctcgg tgatcgtgcc cgtctacaac gagcgcaaca
     1501 ccgtggctga gatcgtgcgc cagatgcgcg ctgtcgagtt acccgtggcg cgtgagatcg
     1561 tcatcgtcga cgacgggagt gacgacggca cccgtgacgt cctgtcccag ctcgccgatt
     1621 ccaccgtcca cgtcgtcctg cacccccaca accgcggcaa aggcgccgcg attcggaccg
     1681 gtctggccca gatcaccggg gatctggtct tgatccaaga cgccgacctc gaatacgacc
     1741 ccgaggactg gccgaagctg atctccccgg tcctcaaggg ccgagcccgg gtggtctacg
     1801 gatcccggtt caccggggag cgtcggaaca tgctgttcct gcactgggtc ggcaaccggt
     1861 tcctgtctct gctgacgaac gtgctctaca acaccaccct ctccgatatg gagacctgct
     1921 acaagctctt cgaccgcgag ctcattgacg ccatggacct tcgatccgac ggattcgact
     1981 tcgaacccga ggtcacggcg aagattctgc gccgcgggat ccgcatctac gaagtgccga
     2041 tctcgtactc gggccgagag ttcgacgaag gcaagaagat cacttggcgg gacggcctgg
     2101 tcgccctaaa gaccttggtc aagtaccggt tcatcaacta acgatccgcg ccgccccgcg
     2161 acacctgagg cgcccgccga cgtcgcggcc ccgcggcgcc gaacccttcg ctgctggcac
     2221 gcgccacgac cacgccctgc tctcggtcct gaggccccga gacgctcact ccgagctgtc
     2281 ggtctctcga cccgaatcga tccacctgta tggtcggtgt cggacggcac gtgtcggacg
     2341 ccgaggggga aggtgtggcc gagaccaagg ggttggcgta ccagccagcc ctcgacgggc
     2401 tgcgggcgct cgcggtcggc tccgtcatcg cgtaccacct cgatgccggc gtcgcccacg
     2461 gtggattcct gggggtcgac accttcttcg tgctgtcggg tttccttatc acgtcgctcc
     2521 tgatcggtga atggggccag agccgcacca tcaacttcgc caagttctgg cttcgccgag
     2581 cccgtcggct gctccccgcg ctgctgctgg tgctgttcgc gatcgcggtc tacgccgcgg
     2641 tcagcgcccc cgcgacccag ctcaaccaac tccgcggtga tgggctcgcc acactcttct
     2701 acggggcgaa ctggcggttt atcgcctccg gccagtcgta cttcacgctt ttcaccaccg
     2761 cgtcgccgct gcggcacatg tggtcgctgg caatcgaaga gcagttctac ctggtttggc
     2821 cgctgatcac gttcgcgtgc ttgtggttgg ctcgtggtcg ccgctggctc ctggcgacgg
     2881 tctgtgtcgt cggcaccgcg gcgtcggcgc tgacgatggc atcgctgtac caacgcgccg
     2941 acccgtcgcg cgcgtactac ggaaccgaca gccgggctca gcttctgctc gtcgggtgcc
     3001 tgctcgcact ggtgctcgcc cgctggtcac cgacgacgac ccgggcccgg accgcggtca
     3061 gcgtcgccgg ggcggtcggt gtcgcgtact gcttcttcgc ctggacccag atcaccgaca
     3121 ccgcctcgtg gatgtaccgc ggcgggtatc tcctcttcgg cctcgccgtc gccgcggtga
     3181 tcacctcagc cgtccaaccc gggcggttcc cggtccgcgc cgccctgtcg gtcggaccgc
     3241 tggtctgggt gggtcgcatc tcgtacggcc tctatctctg gcactggccg gtgatcgtca
     3301 tctgctcacc ggaccgcgtc ggcctcgacg gctggcagtt ggccgtcgtc cggctcgcgc
     3361 tcaccttcgg gatcgcgacc ttgtcctact acctcgtcga gctgcctatc cgtgagggcc
     3421 gcctcttccg tcgccggttc ggtctcgcga tcccgaccgc gttcgtgggc gccggtgtcg
     3481 ccgtgatggt ggcgaccgcc ggcgccgcgc cgcttcccac gtacctgcag ggagccaccc
     3541 acgtcgtgaa ggccgccggc gagtcgacga aggccccgac gccgacgcca ggcgtcgcga
     3601 cgccgggcct gcccgcggct cccccccgcc ggctgctgct cgtcggtgac tcgctcgcgg
     3661 tgagcctcct g
//
LOCUS       DATCCH010000474         2137 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_36241, whole genome shotgun sequence.
ACCESSION   DATCCH010000474 DATCCH010000000
VERSION     DATCCH010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36241"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..1137)
                     /locus_tag="VIJ44_09145"
     CDS             complement(106..1137)
                     /locus_tag="VIJ44_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018331669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY5166104.1"
                     /translation="MELRTLGRSGVQVSVYCLGAMMFGAWGNPDHDDCVRIIHRALDE
                     GINFIDTADVYSAGESEEIVGRAISGRRDDVVLATKFFAPMGSDPNRRGGSRRWILQE
                     VEDSLRRLQTDHLDLYQIHRPDDVVDIDDTLGALSDLVHQGKIRYLGHSTFPADYIVE
                     AQWVAERRGRERFVSEQPPYSILMRGIERSVLPACERYGIGVIPWSPLAGGLLTGRYR
                     KGQEPPEGSRLARGFGARFENERQRQVHEQRLDAVEELLAVAANAGVSLTHLAHAFVL
                     EHPAVTSAIIGPRTMEQLDDVLAGVEVRLDVATLDAIDEVVRPGGDLTAGDPWQPPGL
                     VRANRRRPR"
     gene            1157..1657
                     /locus_tag="VIJ44_09150"
     CDS             1157..1657
                     /locus_tag="VIJ44_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018433255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY5166105.1"
                     /translation="MTITSDELAAREEIRDVLFRYTRGVDRLDLDLVRSCYHTDAHDD
                     HGAFRGDVDGFVDWVASALSYFESTMHFIGNQLIDVEGDVAHTESYCIAYHRRGPRDG
                     EVPHDLVTALRYVDRLERRADRQWKIAERVCVFDWSRRDPVVDQWAMDGAVRGRRGPD
                     DPVWSR"
     gene            complement(1679..>2137)
                     /locus_tag="VIJ44_09155"
     CDS             complement(1679..>2137)
                     /locus_tag="VIJ44_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012998595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase"
                     /protein_id="HEY5166106.1"
                     /translation="NVVMDGYWNQPEDTARAIRDGWFHTGDGGHLDEDHYLVISDRKK
                     DVIITGGENVSSIEVEDCLFSHPSVAEVAVIGVPDDKWGETIKALVVTAEGGDVTEED
                     LIGHCRERLAHYKCPRSVEFRSELARTATGKLQKFKLREPYWTDRDRQVG"
BASE COUNT          341 a          780 c          674 g          342 t
ORIGIN      
        1 ccacaatctg gtcgtcgacg ccggcttcac ctcggccatg acgacgaacc aagtcgactt
       61 cgccgggctc agggccgact aaccctcaag tcggcgccgg ccgcgtcagc gagggcggcg
      121 ccggttggcc cggacgagac cggggggctg ccacggatcc ccagcggtga ggtcgccacc
      181 gggccgcacc acctcgtcga tggcgtccag ggtcgcgaca tcgagccgga cctcgacgcc
      241 ggcgaggaca tcgtcgagct gctccatcgt gcggggcccg atgatcgccg acgtcaccgc
      301 cggatgctcg agcacgaagg cgtgcgccag gtgggtcaaa ctcacgccgg cgttggccgc
      361 cacagccaag agctcctcca ccgcgtcgag gcgctgctcg tggacctggc gctgccgctc
      421 gttctcgaac cgggcgccga agccccgcgc cagccgcgac ccctccgggg gctcctggcc
      481 cttccggtac cggccggtca gcaaccctcc cgcgagcgga ctccacggga tcaccccgat
      541 tccgtagcgc tcacatgcgg gcagcaccga ccgctcgatc ccacgcatca ggatcgaata
      601 tggcggctgc tccgacacga accgctctcg ccctcgccgc tccgcgaccc actgcgcctc
      661 gacgatgtag tcggccggga acgtcgagtg gcccaggtat cggatcttcc cctgatgcac
      721 cagatccgag agcgcaccga gcgtgtcatc gatgtccacc acatcatcgg gacgatggat
      781 ctggtacaga tcgaggtggt cagtctggag tcgccgcaga ctgtcttcga cctcttgaag
      841 aatccaccgg cgcgacccgc cccgccggtt cgggtccgac cccatcggcg cgaagaactt
      901 cgtcgccaga accacgtcgt cgcggcggcc gctgatcgcc cggcccacga tctcctccga
      961 ctccccggcc gagtagacgt cggcggtgtc gatgaagttg atcccctcgt cgagggcccg
     1021 gtgaatgatg cggacacaat cgtcatggtc agggttgccc cacgctccga acatcatcgc
     1081 cccgaggcaa tacacactga cctgcacgcc gctacgccca agcgtcctca gctccatccc
     1141 cgtaccctag gtgcccatga cgatcaccag cgatgagctc gccgcccggg aagagatccg
     1201 tgacgtcctg ttccgctaca cgcgaggagt cgaccggctc gacctcgacc tcgtccgctc
     1261 ctgctaccac accgacgcgc acgacgacca cggagcgttc cgcggcgatg tcgacgggtt
     1321 cgtggactgg gtcgccagcg cattgtcgta ctttgagtca accatgcact tcatcggcaa
     1381 ccagctcatc gacgtcgagg gcgacgtggc ccacaccgag tcgtactgca tcgcgtatca
     1441 ccgtcgggga ccccgcgacg gcgaagttcc gcacgacctg gtgaccgcgc tgcgttacgt
     1501 cgaccggctc gagcgccgcg ccgaccggca gtggaagatc gccgagcggg tgtgcgtgtt
     1561 cgactggagc cgccgcgacc cggtcgtcga ccagtgggcc atggacggcg cggtgcgcgg
     1621 gcgacgcggc cccgacgatc cggtctggag ccgctaggcc gcctcggcgg cggtgtcgtc
     1681 agccgacctg acggtcccgg tcggtccagt acggctcccg cagcttgaac ttctgcagct
     1741 tgccggtcgc ggtgcgggcc agctcgcttc ggaactcgac cgagcgcggg cacttgtagt
     1801 gggcgaggcg ctcccggcag tgcccgatca ggtcctcctc ggtgacgtcg cccccttcgg
     1861 cggtgactac gagggccttg atcgtctcac cccacttgtc gtcggggacg ccgatcacgg
     1921 ccacctcggc cacactggga tgggagaaca ggcagtcctc cacctcgatc gacgagacgt
     1981 tctcgccacc ggtgatgatc acgtccttct tccggtccga gatgacgaga tagtggtcct
     2041 cgtcgaggtg accgccgtcc ccggtgtgga accagccgtc ccggatcgcc cgggccgtgt
     2101 cctcgggttg gttccagtag ccgtccatca caacgtt
//
LOCUS       DATCCH010000475         2094 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_36734, whole genome shotgun sequence.
ACCESSION   DATCCH010000475 DATCCH010000000
VERSION     DATCCH010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36734"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..161)
                     /gene="argC"
                     /locus_tag="VIJ44_09160"
     CDS             complement(<1..161)
                     /gene="argC"
                     /locus_tag="VIJ44_09160"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:NF013298.4"
                     /note="catalyzes the reduction of N-acetyl-5-glutamyl
                     phosphate to N-acetyl-L-glutamate 5-semialdehyde in
                     arginine biosynthesis and the reduction of
                     N-acetyl-gamma-aminoadipyl-phosphate to
                     N-acetyl-L-aminoadipate-semialdehyde in lysine
                     biosynthesis; involved in both the arginine and lysine
                     biosynthetic pathways; lysine is produced via the AAA
                     pathway, lysine from alpha-aminoadipate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEY5166107.1"
                     /translation="MICAMAYRAGIVGGSGYAGAELLRLLAHHPDLEVVTATGDRRAG
                     IRVADLYPSL"
     gene            170..562
                     /locus_tag="VIJ44_09165"
     CDS             170..562
                     /locus_tag="VIJ44_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5166108.1"
                     /translation="MHSERVGLLAEGVELGPLPLTVSEAANDRYWAGAGVEHPARQAG
                     WLYPPMAANLTVLLVQTRLTDPMLHTAQRLECHRGGRAPADLIVEGIVTRRFRKRERD
                     YMVVEAVVSVAGEEPLWTSVATFTPVAR"
     gene            523..927
                     /locus_tag="VIJ44_09170"
     CDS             523..927
                     /locus_tag="VIJ44_09170"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEY5166109.1"
                     /translation="MDVGRDLHAGGAMTARERTRTLMLTAERLRWYSRRGNFHADRDT
                     ARDLGLPDLVAQGMQVAGPAYGLLLDEWGDEWLSTGMIELTFVGMVTAEETVEARVEV
                     DGDDAIVAITGVGDGGVRVVGRAARRGAPGSY"
     gene            complement(921..>2094)
                     /locus_tag="VIJ44_09175"
     CDS             complement(921..>2094)
                     /locus_tag="VIJ44_09175"
                     /inference="COORDINATES: protein motif:HMM:NF036799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5166110.1"
                     /translation="RITLRTSRVNAILGTELRDTDVLDALRPLEVDVTGNGEDIVAIP
                     PTFRPDLEREIDLIEEVARRVGFDRIGRTVARPRDQVGGLTAAQTERRHVTDALVGVG
                     AAEVTTIPLVAPALLAMLGVGDTVALANPLRADESVLRASLLPGLLTVAAANTARGRP
                     DLALFEVGAVFFPPDTDQLLPVERLDVGALLTGTLERRPLEADRPVDAYDAVDLVRVV
                     ADTLELDDWDLEAATISGYHPGRAARIRAGGAVVGAVGELHRDVLATVGLAGPVVGFD
                     ADLEALRAARRRDRTFRPLSPYPPSTIDLAFVVDDTVPAAAITKTLRDAAEGLAEEVR
                     AFDEFHSPTLGAGKRSVGFALRFRAPDRTLTDADVATLRRRCIDAVTTAYGATLRQ"
BASE COUNT          292 a          657 c          864 g          281 t
ORIGIN      
        1 agggacgggt acaggtcggc gacccggatc ccggcccgac ggtcaccggt cgcggtgacc
       61 acctcgagat caggatggtg ggccaggagg cggagcagct cggcgccggc gtagcccgac
      121 ccgcccacga tccccgcgcg atacgccatg gcgcatatca tacacactga tgcatagcga
      181 gcgggtgggt ttgctggccg agggggtcga gctgggtccg cttcccctca cggtcagcga
      241 ggcggccaac gaccggtatt gggccggagc cggcgtggag catccggccc gacaggccgg
      301 gtggttgtac ccgccgatgg cggcgaacct gaccgtcttg ttggtgcaga cccggctgac
      361 cgacccgatg ctgcacaccg cgcagcggct cgagtgtcac cgcggcggac gagcacccgc
      421 ggacctgatc gtcgagggga tcgtgacccg ccggtttcgg aaacgcgaac gcgactacat
      481 ggtcgtcgag gcggtggtgt cggtcgccgg cgaggagccg ctgtggacgt cggtcgcgac
      541 cttcacgccg gtggcgcgat gaccgcacgc gagcggacgc gcacattgat gttgaccgcg
      601 gagcggctgc gctggtactc gcggcgcggc aactttcatg ccgatcgcga cacggcgcgg
      661 gacctcggcc tccctgatct ggtggcgcag ggcatgcagg tggccggccc ggcgtacggg
      721 ttgctcctcg acgagtgggg cgatgagtgg ctcagcaccg ggatgatcga gctgaccttc
      781 gtcgggatgg tgaccgcgga ggagacggtg gaggcccgcg tcgaggtcga cggggacgac
      841 gccatcgtgg ccatcaccgg ggtcggcgac ggcggggtgc gggtcgtcgg tcgcgccgcg
      901 cgtcgcggtg ccccggggag ctactgacgc agcgtcgcgc cgtaggcggt ggtgacggcg
      961 tcgatgcagc gtcgtcggag cgtggcgacg tcggcgtcgg tgagcgtccg gtcgggggcc
     1021 cggaaccgca acgcgaagcc gacgctccgc ttgcccgctc cgagcgtcgg ggagtggaac
     1081 tcatcgaagg cgcgcacctc ttcggcgagc ccttcagcgg cgtcgcggag ggtcttggtg
     1141 atcgccgcgg caggcacggt gtcgtcgacg acgaaggcga ggtcgatcgt cgacggcggg
     1201 tagggcgaga gcggtcggaa ggtacggtcg cggcggcggg cggcgcgcag cgcctcgagg
     1261 tcggcgtcga agccgacgac cgggccggcc agcccgaccg tggcgaggac atcgcggtgg
     1321 agctccccca ccgcgccgac gacggcgcca ccggcgcgga ttcgcgcggc ccgaccgggg
     1381 tggtacccgg agatggtcgc ggcttccagg tcccagtcgt cgagctcgag ggtgtcagcg
     1441 acgacgcgga ccaggtcgac cgcgtcgtag gcgtccacgg gccggtcggc ctcgaggggg
     1501 cggcgctcca gggtgccggt caggagagcg ccaacgtcga ggcgctcgac cgggaggagc
     1561 tgatcggtgt ccggggggaa gaagaccgcc ccgacctcga acagcgcgag gtcggggcga
     1621 cctcgcgcgg tgttcgcggc cgcgacggtg aggagtccgg ggagcagcga ggcccggagc
     1681 accgattcgt cggcgcggag cgggttggcg agcgcgacgg tgtcgccgac tccgagcatg
     1741 gcgagcagcg cgggggcgac cagtgggatg gtggtgacct cggcggcgcc gacgccgacg
     1801 agcgcgtcgg tgacgtggcg gcgttcggtc tgggctgcgg tgaggccacc gacctggtcg
     1861 cgggggcggg ccacggtccg cccgatgcgg tcgaagccga cgcgccgggc gacctcttcg
     1921 atcaggtcga tctcccgctc gaggtcaggg cgaaacgtgg gggggatcgc gacgatgtcc
     1981 tcgccgttgc cggtgacgtc gacctcgagg gggcggaggg cgtcgaggac gtcggtgtcg
     2041 cgcagctcgg tgccgaggat ggcgttgacg cggctggtgc gaagcgtgat gcgg
//
LOCUS       DATCCH010000476         2315 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_36776, whole genome shotgun sequence.
ACCESSION   DATCCH010000476 DATCCH010000000
VERSION     DATCCH010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36776"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..972
                     /gene="folP"
                     /locus_tag="VIJ44_09180"
     CDS             121..972
                     /gene="folP"
                     /locus_tag="VIJ44_09180"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEY5166111.1"
                     /translation="MPWAYQLGDRVHDLTDRALVMGILNRTPDSFYDRGATFDLDDLM
                     RRAGQLIGDGADLLDVGGVKAGPGPDVDEAEELDRVVPTIEALHDRVEVPISVDTWRA
                     SVLDAACDAGAVIGNDISGFADPHYLAVAARHQASVVATHIRLAPRVPDPEPRYVDLL
                     RDVATFLTERAEQARAAGLSADQIALDAGLDLGKTPAMSAQLLRETDRLADLGYTLLL
                     SASNKRFLGDLLGLDLHDRRDVSLAAAAYGVAHGARIIRVHDVAGTIRVCRTVEAILT
                     EAPVAVR"
     gene            969..2000
                     /locus_tag="VIJ44_09185"
     CDS             969..2000
                     /locus_tag="VIJ44_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5166112.1"
                     /translation="MSVHLVTGNDPVLRGDAVTSLVDELLDGGDRSLTVEEFTAAGRG
                     DTDDVVGNDEVVGAVVNAASSPPFMTSQRIVIVRDIGTLRAADAVPLVAYLDDPLSST
                     ELILVVGGGKTPDALDKGARAHGTVTGPTSEKTADVLAFELGGHDLDLTRDATDAIRA
                     HVGDDAGLVPGLVATLASAHPPEARLDVDDVTPYLGEAGSIPVWDLTNAIERGDIPGA
                     LATVRRLLTVTSPRQPKPMHPLQITAMLHGHYRRLLKLDDPAVRTNEDAAAAIGGRTN
                     PRAAGFRLRQARALGTDGLRRAFDLLAHADLDLKGARAIPEDAVVELLVARLAALGSP
                     ARRRTTGAR"
     gene            complement(2061..>2315)
                     /gene="rpsT"
                     /locus_tag="VIJ44_09190"
     CDS             complement(2061..>2315)
                     /gene="rpsT"
                     /locus_tag="VIJ44_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEY5166113.1"
                     /translation="KGQIKRNRQNETRRLRNKAVRTELKSRVKGAVVAAAEGSPDADD
                     QIRIAQKRLNKAGAKGRIHPRQASRRTSRLMKRAAKGAQT"
BASE COUNT          321 a          924 c          739 g          331 t
ORIGIN      
        1 ccgtcgacca ccgccaccac gaccacgctc ccgacggttc cagcctcgac ggccgcgccg
       61 acgcggtgat gccggccgcg acgagcccgg cttcgaatcg tcggcgtagg ctccgccgag
      121 atgccctggg cctatcaact cggcgaccgg gtccacgacc tcaccgaccg ggccctggtg
      181 atgggaatcc tgaaccggac ccccgactcg ttctatgacc gcggcgccac gtttgacctc
      241 gacgacctca tgcgacgcgc cggccagctc atcggcgacg gcgctgacct cctcgacgtc
      301 ggcggcgtga aggccggccc cggacccgac gtcgacgaag ccgaggagct cgaccgtgtc
      361 gtcccgacca tcgaggccct ccacgaccga gtcgaggtcc cgatctccgt cgacacctgg
      421 cgggcgtcgg tgctcgacgc cgcctgcgat gcgggggccg tgatcggcaa cgacatcagc
      481 gggttcgccg acccgcacta cctggcggtc gcggctcgac accaggcgtc ggtggtcgcc
      541 acccacatcc ggctcgcacc ccgcgtcccc gatcccgaac cccgctacgt cgacctgctc
      601 cgcgacgtcg ccacgttcct gaccgagcgg gccgagcagg cccgcgccgc ggggctgtcg
      661 gcggaccaaa tcgccctcga cgccggcctc gacctcggca agacccccgc catgagcgcc
      721 cagctcctcc gcgagaccga ccggctcgcc gacctcggct acaccctctt gctgtcggcg
      781 tcgaacaagc ggttcctcgg tgatctgctc ggcctcgacc tccacgaccg gcgcgacgtg
      841 tcgctcgccg ccgcggcgta cggcgtcgcc cacggcgccc gcatcatccg ggtccacgac
      901 gtggccggca ccatccgggt gtgccgcacc gtcgaggcca tcctcaccga agcaccggtg
      961 gcagtccgat gagcgtccac ctcgtgaccg gcaacgatcc ggtcctgcgc ggcgacgcgg
     1021 tcacctccct cgtcgacgag ctcctcgacg gcggcgaccg gtccctcacc gtcgaggagt
     1081 tcaccgccgc cggccggggc gacaccgacg acgtcgtcgg caacgacgaa gtggttggcg
     1141 cggtggtcaa cgcggcgtcg agcccaccgt tcatgacctc tcagcggatc gtcatcgtcc
     1201 gagacatcgg gactctccgc gccgccgacg ccgtgccgct cgtcgcgtac cttgacgatc
     1261 cgctgtcgag caccgagctg atcctggtcg tgggcggcgg caagactccc gacgcgctcg
     1321 acaagggtgc acgagcccac ggcaccgtga ccggcccgac atcggagaag accgccgacg
     1381 tcctcgcgtt cgagttgggc ggccacgacc ttgacctgac ccgcgacgcc accgacgcca
     1441 tccgcgctca tgtcggtgac gacgccggcc tcgtgccggg cctggtggcg acgctggcca
     1501 gcgcgcaccc accggaggcg cgtctcgacg tcgacgatgt caccccgtac ctcggggagg
     1561 ccggctcgat cccggtctgg gacctcacga acgccatcga gcgaggcgac atccccgggg
     1621 cgctcgccac cgtccggcgg ctcctcaccg tcacgagccc tcgccagccc aagccgatgc
     1681 acccgttgca gatcaccgcg atgctgcacg gccactaccg gcgtctcctc aagctcgatg
     1741 accccgcggt gcggaccaac gaggacgccg ccgccgcgat cgggggccgc accaacccgc
     1801 gagccgccgg gtttcggctc cgccaggccc gggcgctggg caccgacggc ctccgtcggg
     1861 ccttcgacct cctggcccac gccgacctgg atctcaaagg ggcgcgtgcg atccccgagg
     1921 acgccgtcgt ggagctgctc gtcgcccgtc tcgctgccct cggctcaccg gcgcggcgcc
     1981 gcacgaccgg agcgcgttag ccccacgccc gatgtcgcga gccggctcaa cccatccgcg
     2041 ggccagaccg ggcgctgacg ctaggtctgt gcgcccttcg cggcgcgttt catgagccgc
     2101 gacgtccgtc gggacgcctg acgggggtgg atccgaccct tcgcgccggc cttgttgagc
     2161 cgcttctgag cgatccgaat ctggtcgtcg gcgtccgggg agccttcggc ggccgcgacc
     2221 accgcccctt tgaccctcga cttgagctcg gtgcgaaccg ccttgttccg caaccgtcgc
     2281 gtctcgtttt gacggttccg cttgatctga ccctt
//
LOCUS       DATCCH010000477         2235 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_412421, whole genome shotgun sequence.
ACCESSION   DATCCH010000477 DATCCH010000000
VERSION     DATCCH010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412421"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VIJ44_09195"
     CDS             complement(<1..195)
                     /locus_tag="VIJ44_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5166114.1"
                     /translation="MTVLAGVFLGVASAFAVGDWLAIAKASTRLEYLCKPGATVALVG
                     VAATLDAAHGDTRAWFVAALV"
     gene            333..1181
                     /locus_tag="VIJ44_09200"
     CDS             333..1181
                     /locus_tag="VIJ44_09200"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEY5166115.1"
                     /translation="MLKRVRGQLVGLPGVSAALDVQRRYTEERGSALAASITLYGFLA
                     LFAVTALAIAALGFLSAGGAHVARDLPNRLGVSGEAARLVHRAVDSARRSRAVATVVG
                     LVGLAWTGTSLAIVVGDAYNAAWHVDRRGLVDRARGLAWLAGAVVPIAAGAAATALWS
                     PLPGFFAPLVVCVSIATNAALFAWTSWILPNRRVPVGALIPAAVLGGVALEALKVLGA
                     YAVPHLVARSSEIYGTIGVVFALLAWLLVFGRVVVYVPIVEARRWEHTHGDQRVELEV
                     PALPAR"
     gene            complement(1223..1828)
                     /gene="pdxH"
                     /locus_tag="VIJ44_09205"
     CDS             complement(1223..1828)
                     /gene="pdxH"
                     /locus_tag="VIJ44_09205"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041328297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEY5166116.1"
                     /translation="MTAGLVEQDASADPMSQFRRWFGEAEAAGVVDRDAMVVATADRS
                     GNPSARVVLLRGVDERGFRFYTNFESAKGRELAERPRAALVLHWREQGRQVRITGAAT
                     RLGDEESRAYWRHRPRASRLSAWASRQSEPIADRPTLETAAAEIADRFGVEDVPLPPF
                     WGGYLVTPDAVEFWQHRDDRLHDRLRYQRLNEGWVRQRLQP"
     gene            1969..>2235
                     /locus_tag="VIJ44_09210"
     CDS             1969..>2235
                     /locus_tag="VIJ44_09210"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY5166117.1"
                     /translation="MAFHHVAITTRDPEATHAFYTEAMGFELVRVEAAPAAEGGWARH
                     LFYDTGNGECLAVWDLHDNPAVPTEFDTSISRGLGLPTWTNHLAF"
BASE COUNT          284 a          816 c          798 g          337 t
ORIGIN      
        1 caccagcgcg gcgacgaacc aggcccgggt atcgccgtgg gcggcgtcga gcgtcgcggc
       61 caccccgacc aacgccacgg tcgcgcccgg cttgcacagg tattcgaggc gggtcgaggc
      121 cttggcgatc gccagccagt cgccgaccgc gaaggcgctc gcgacgccga ggaacactcc
      181 ggcgaggacc gtcacagctg ggttctactc gcgaacaccc accgcgcccg tacgatccgg
      241 tccgtgcgcc gcgggtgggt gaaccgacac gccgggaatc gtcccgggtg gggcgaccgc
      301 cggttgcctc gcggcaggcc tcagggccgt gcgtgctgaa gcgtgtccgc ggccagctcg
      361 tcggcctgcc tggggtctcc gcggcccttg acgtccagcg tcgctacacc gaggagcgag
      421 gctcggccct cgcggcatcg atcaccttgt acgggttcct cgccctgttc gcggtgacgg
      481 cgctcgcgat cgcggcgctc gggtttctct ccgcgggcgg ggcccacgtg gcgcgggacc
      541 tgccgaaccg gctcggcgtg agcggtgagg ccgcccgcct ggtgcatcgg gccgtcgact
      601 cggcgcgccg gagccgggcg gtcgccaccg tcgtggggct ggtcggtctg gcttggaccg
      661 gcaccagcct cgccatcgtc gtcggcgacg cgtacaacgc ggcgtggcat gtcgaccggc
      721 ggggtctcgt ggaccgggcc cgcgggctgg cttggctggc cggcgcggtg gtgccgatcg
      781 ccgcgggcgc ggccgcgacc gcgctgtgga gtccgttgcc cggattcttt gcgcctctgg
      841 tcgtgtgcgt gtcgatcgcg acgaacgcgg ccctgttcgc ctggacgtcg tggatcctgc
      901 cgaaccgccg ggttccggtc ggggcgctga tccccgcggc ggtgctcggc ggggtcgcgc
      961 tcgaggccct gaaggtgctc ggcgcgtacg cggtcccgca tctcgtggcc cgctcctcgg
     1021 agatctacgg aacgatcggg gtcgtcttcg cgttgctcgc ctggctgctc gtcttcggac
     1081 gggtcgtggt ctacgtcccg atcgtcgagg cgcgccggtg ggagcacacc cacggcgacc
     1141 agcgggtcga gctggaggtg ccggcgcttc ccgcccgttg accgagaacg ctcgcgtcgc
     1201 cggcaaaccg tcggcgttgt cctcagggct ggagccgttg acggacccag ccctcgttca
     1261 agcgttgata gcgaagccga tcgtggaggc ggtcgtcgcg gtgctgccag aactcgacgg
     1321 cgtcgggagt caccaggtag ccaccccaga acggcggcaa cgggacgtcc tcgacaccga
     1381 accggtcggc gatctcggcc gccgccgtct cgagcgtcgg gcggtcggcg atgggctcac
     1441 tttgacgtga cgcccaggcg ctcagccggc tggcccgggg acggtgtctc caatacgccc
     1501 gtgactcctc gtcgccgagg cgggtcgccg ctccggtgat ccgcacctgt cggccctgct
     1561 cacgccagtg gagcacgagc gccgctcggg gacgctcggc aagctcacga cccttcgcgc
     1621 tttcgaagtt cgtgtagaag cggaagcccc gctcgtcgac gccgcggagc agcaccactc
     1681 gggcgctcgg gttcccgctg cggtccgcgg tcgccaccac catcgcgtcg cgatcgacga
     1741 ccccggccgc ttccgcttcg ccgaaccagc ggcggaactg cgacatcggg tccgccgacg
     1801 cgtcctgctc caccagcccg gccgtcacgg gccatcaccc ggcccgagcc gagccctgcg
     1861 gttcccgccg ggcagaccgg atcgtgacac tcaggccacg gtagtgatcc gagggccgcc
     1921 caccgcgcga agatcgtggc aacgtcacca ccaacgagga gccgccgtat ggcgttccat
     1981 cacgtcgcga tcaccacccg cgatcccgag gcgacccatg ccttctacac cgaggcgatg
     2041 gggttcgagc tggtccgcgt cgaggccgct ccggccgcgg aggggggatg ggcccgccat
     2101 ctcttctacg acaccgggaa cggcgagtgc ctggcggtgt gggacctcca cgacaacccc
     2161 gcggtgccca ccgagttcga cacctcgatc agccgagggc tcggcctgcc gacctggacg
     2221 aaccacctgg ccttc
//
LOCUS       DATCCH010000478         2430 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_37494, whole genome shotgun sequence.
ACCESSION   DATCCH010000478 DATCCH010000000
VERSION     DATCCH010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37494"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /locus_tag="VIJ44_09215"
     CDS             <1..919
                     /locus_tag="VIJ44_09215"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5166118.1"
                     /translation="RPPRATNYARRHAGVRAHNRWLADFCAEAPGRRAGIGQIFLNDI
                     DDAVADVRWIKEHQLRGGALLPGRPDDSEFPPLYDAVYDPLWSVCEELEVPVTHHSGQ
                     GSPDYGRNDAASVMWLVETQWFSHRPFWQLIMGGVFERHPDLSFVVTEQGCSWIPDTL
                     RMLDAYHAQMASGRVGELKYTEDQRLSLTPSEYFARNCYVGVSFPSPAESRAMRTVGL
                     DRVMWGSDYPHNESTYPHTTAGLRRAFADWDPVEVRQVTSENTARIYGFDLDALSPLA
                     VDVGPSVEEVTRPLDTVPADSASPAFTRR"
     gene            complement(937..>2430)
                     /locus_tag="VIJ44_09220"
     CDS             complement(937..>2430)
                     /locus_tag="VIJ44_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020549658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY5166119.1"
                     /translation="MLIDESDRTVTFGEFRDRAARVAAGLHALGVGEGTAVSWQLPTR
                     IDTIVLSAALGRVGAVQNPIIHLYREREVGFALRETGARLFFVPGVWRGFDYPALAAR
                     VSAELAEAPSIVVVADGLPDGDPSVLPPPSAGRPAAEAPVRWIYYTSGSTADPKGVCH
                     TDPSLIAGGWGLAVALDMSPDDVGSIAFPYAHIGGPDYLVTMLSLGFPAVLIEAFSAA
                     EVLPVLRRRGVTMVGGSTAFYLAFLTEQRKTPGEPILPSLRLMSGGGAPKPPELHYEV
                     QREIGGRGTVHGYGMTEVPMIAQGSPHDTDEQLANTDGKPVLGIDVRIVTRDGDVASL
                     DEEGEIRLRGPMVFRRYTDPALTSDAFDPDGYFRTGDLGKLRADGHLVVTGRLKDVIV
                     RKGENVSAKEIEDILYTHPKVDDVAVVGLPDPDRGERVCAVVQIADEDQRFSFEEMVA
                     HCRDAGLMVQKVPEQLELRAEMPRAATGKIVKAKLRAEYAARPWSPA"
BASE COUNT          384 a          862 c          826 g          358 t
ORIGIN      
        1 ccgaccaccc cgcgccacga actacgcgcg gcgccacgcc ggggtccggg cccacaatcg
       61 gtggctggcc gatttctgcg ccgaggcgcc cggtcggcgg gccgggatcg gtcagatctt
      121 cctcaacgac atcgacgacg ccgtcgcgga cgtgcggtgg atcaaggagc accagctgcg
      181 gggtggtgcc ctcctccccg gccgacccga cgattccgag ttcccgcccc tctacgacgc
      241 ggtctatgac ccgctgtggt cggtgtgtga agagctcgag gtgccggtca cccaccactc
      301 ggggcaaggg agccccgatt acggccgcaa cgacgcggcg agcgtgatgt ggctcgtcga
      361 gacccagtgg ttctcgcacc ggcctttctg gcagctgatc atgggcggcg tcttcgaacg
      421 gcatcccgac ttgtcgttcg tcgtcaccga acaaggctgc tcgtggatcc ccgacacgct
      481 ccggatgctc gacgcctacc acgcccagat ggcgtcggga cgcgtcgggg aactgaagta
      541 caccgaggac cagcggctct cgctcacccc gagcgagtac ttcgcccgca actgttacgt
      601 cggggtgagc ttcccgagcc ccgccgaatc gcgagccatg cgcaccgtgg ggctcgaccg
      661 cgtcatgtgg ggcagcgact acccccacaa cgagagcacc tatccgcaca ccaccgcggg
      721 gctgcgacgc gccttcgccg actgggatcc cgtcgaggtg cgccaggtca cgagcgagaa
      781 cactgcccgc atctacggct tcgacctcga cgccctcagc ccgctcgccg tcgacgtcgg
      841 gccaagcgtc gaggaagtca cgcggccgct tgacaccgtg ccggccgact cggcgagtcc
      901 cgccttcacc cgccggtagt cagcaccgac gcttcgtcac gccgggctcc acggtcgagc
      961 cgcgtactcg gcccggagct tcgccttcac gatcttgccc gtcgcggcgc ggggcatctc
     1021 ggcgcgcagc tcgagctgtt cgggaacctt ctggaccatg agcccggcgt cgcggcagtg
     1081 cgccaccatc tcctcgaacg agaaccgctg gtcctcatcg gcgatctgga ccaccgcgca
     1141 gacccgctcc ccgcgatcgg ggtcggggag cccgacgacc gcgacgtcgt cgaccttggg
     1201 gtgcgtgtag aggatgtcct cgatctcttt ggcgctgacg ttctcgccct tgcggacgat
     1261 gacatccttg agccggccgg tgacgacgag atgaccgtcg gcgcgcagct tcccgagatc
     1321 gccggtccgg aagtagccgt cggggtcgaa cgcgtcggag gtgagagccg ggtcggtgta
     1381 cctgcggaac accatcggcc cccggaggcg gatctcgccc tcctcgtcga gcgacgcgac
     1441 gtcgccgtca cgggtcacga tccgcacgtc gataccgagg acgggcttcc cgtcggtgtt
     1501 ggcgagctgc tcgtcggtgt cgtggggcga gccttgagcg atcatgggca cctcggtcat
     1561 gccgtagccg tgcaccgtgc cgcgaccgcc gatctcgcgc tgcacctcgt agtgcagctc
     1621 gggcggcttc ggggcccctc cgcccgacat cagccgcaag ctgggaagga tcggttcgcc
     1681 tggcgtcttg cgttgctcgg tgagaaacgc aaggtagaac gcggtgctcc cgccgaccat
     1741 cgtcacgccg cggcggcgaa ggaccggcag gacctccgcg gccgagaacg cctcgatgag
     1801 caccgcggga aacccgaggc tcagcatcgt cacgaggtag tcggggccgc cgatatgggc
     1861 gtacgggaac gcgatcgaac cgacgtcgtc gggtgacatg tcgagcgcca ccgccaggcc
     1921 ccaccccccg gcgatgagcg acgggtcggt gtggcacacc cctttgggat cggcggtgct
     1981 ccccgacgtg taatagatcc aacggaccgg ggcctcagct gccgggcggc ccgccgacgg
     2041 cggcgggagc accgacgggt ccccatcggg caagccgtcg gcaacgacca cgatggaagg
     2101 ggcctcggca agctcggcgg agacacgcgc cgcgagagcg gggtagtcga agccgcgcca
     2161 cacgcccggg acgaagaaca gacgagctcc ggtctcgcgg agcgcgaaac cgacctcgcg
     2221 ctcgcggtac aggtggatga tggggttctg gactgccccc acgcgaccga gagccgctga
     2281 gagcacgatc gtgtcgatgc gcgtcggcaa ctgccaggac actgctgttc cctcgccgac
     2341 gccgagggcg tgcaggcccg ccgcgacccg ggcggcccgg tctcggaact cgccgaaggt
     2401 gaccgtgcgg tccgactcat cgatgagcat
//
LOCUS       DATCCH010000479         2057 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_162967, whole genome shotgun sequence.
ACCESSION   DATCCH010000479 DATCCH010000000
VERSION     DATCCH010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162967"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="VIJ44_09225"
     CDS             <1..394
                     /locus_tag="VIJ44_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5166120.1"
                     /translation="SRARLDYPGVALGQTRCPKAVDKRRGASFVCTVPVGNLKLQVRV
                     AQRDANGHIQLAAQQAVIQKTSAEQFVAQHASISATVSCGTQPVLVLAPGARFPCSVS
                     FTDGTMQTVSVRVVDTAGTVVIETPAKP"
     gene            complement(435..1367)
                     /locus_tag="VIJ44_09230"
     CDS             complement(435..1367)
                     /locus_tag="VIJ44_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5166121.1"
                     /translation="MAGLVGSIAAGASTPRRPSTTSTTTPPTPTTTPPAPAAQAPGGS
                     VAWHPAGRQVLGQTVLYLGSLGGADLAWMPPSLVRPVVVPGSGDPGGPWPWGGQVDPG
                     NRPNLVAAFNGGFKWSDFSGGVLAFGHSSRDLAAGQASFVVFADGSFTVGLWGRDVNP
                     GPNVVAVRQNLSMLVDGGAPTAAAGSSGSWGASVGGVATMRSAVGVDAKGGLVWAGGR
                     LSPVDLANALVAGGAIRGMEMDINPAWVNFNLYQPGLDGVVHGAPVYGATGPDRYLSS
                     NSRDFIAVLIRAVVVPGAPGKVGLSPVDGPVHIP"
     gene            complement(1540..>2057)
                     /locus_tag="VIJ44_09235"
     CDS             complement(1540..>2057)
                     /locus_tag="VIJ44_09235"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEY5166122.1"
                     /translation="ARSRSEPATEEGAKHPPLINPTRARRPSPRRSVMADMNDFNRSI
                     IDEFRANNGKVGGQFGGAPMLLLTTTGAKSGQSRTVPVVYLPDGNRYLVFASKAGAPT
                     NPDWYHNLVANPTVSVEVGSDRFDANAVVLTGDERDRFYAKQAELFPGFQEYQDNTTR
                     VIPVVALQPQA"
BASE COUNT          308 a          744 c          723 g          282 t
ORIGIN      
        1 ctcccgcgcc cgcctcgact accccggcgt cgcgctcggc cagacgcgct gccccaaggc
       61 agtcgacaag cgacgcggcg cgtcattcgt ctgcaccgtc ccggtgggca acctgaaatt
      121 acaggtccgg gtcgcgcaac gtgacgccaa cggccacatc cagctcgcag cccaacaggc
      181 ggtgatccaa aagacgtctg ccgagcaatt cgtggcccag cacgcttcga tctccgccac
      241 ggtcagctgc gggacccagc ccgtgctggt cttggcgccc ggcgcccgct tcccatgctc
      301 ggtgagcttc accgatggca cgatgcaaac ggtgtcagtg cgggtcgtcg acaccgccgg
      361 caccgtcgtc atcgaaacgc cggccaagcc gtaaggcgcc cgagaccggg gccgggctgg
      421 ccaccgccga cggactacgg aatgtgcacc ggcccgtcaa ccggtgacag ccccaccttg
      481 cctggggcac cggggacgac cacagcgcgg atcagcaccg cgatgaagtc ccggctgttc
      541 gatgacaggt accggtcggg gccggtcgcc ccgtacaccg gcgccccgtg cacgaccccg
      601 tcgaggcccg gctggtacag gttgaagttc acccacgccg ggttgatgtc catctccatg
      661 ccacggatgg cgccaccggc caccagcgcg ttcgccaggt cgacgggact gaggcgaccc
      721 ccggcccaca ccaggccgcc cttggcgtcc acgccgacgg cggatcgcat cgtcgcgacg
      781 ccccccaccg acgcgcccca cgagcccgag gacccggccg cggcggtggg cgcgccaccg
      841 tcgaccagca tcgagaggtt ctggcggacc gcgacgacat tcgggccggg attgacgtcg
      901 cggccccaca gcccgacggt gaacgaaccg tcggcgaaca cgacgaagga cgcctgacct
      961 gccgcgaggt cacgggacga gtggccgaac gcgaggaccc caccgctgaa gtcgctccac
     1021 ttgaagccgc cattgaacgc cgccaccagg ttggggcggt tccccggatc gacctggcct
     1081 ccccacggcc acgggccgcc cggatcgccc gagcccggga caacgacggg ccgcaccagg
     1141 ctcgggggca tccacgccag gtcggcgccg cccagcgacc cgaggtacaa gacggtctgg
     1201 ccgagcactt ggcggcctgc cgggtgccag gcgaccgacc cgccgggagc ctgcgccgcg
     1261 ggcgccgggg gcgtcgtcgt cggggtcggg ggggtggtcg tcgacgtcgt cgacgggcgg
     1321 cggggcgtgg acgccccggc cgcgatcgac cccaccagtc cggccatcag gatcgtcgcg
     1381 gccgcgacga tcctgatggc gacgtgcccg catcgacccg ttcgcacggc ggaagtatgg
     1441 cgtgcgggga ggccgaccgg ctcgcgcggc gccgcggtac ggcgagcccg ggcttcctcg
     1501 gtcaggtcgc gcgtcgcgtc ccgccgttct cgtcggcggc tacgcctgag gttgcaacgc
     1561 gacgacgggg atgacccgcg tcgtgttgtc ctggtactcc tggaagccgg gaaacagctc
     1621 ggcctgcttg gcgtagaacc ggtcgcgctc gtcgccggtc aagacgaccg cgttggcgtc
     1681 gaagcggtcg gacccgactt ccactgacac cgtggggttc gcgaccaggt tgtggtacca
     1741 gtccgggttc gtgggggccc cggccttcga cgcgaagacg aggtagcggt tcccgtcggg
     1801 gaggtacacg accggaacgg tccgagactg gccgctcttg gcgccggtcg tcgtgagcag
     1861 cagcatcggc gcgcccccga attggccgcc gactttgccg ttgttggctc ggaactcgtc
     1921 gatgatggac cggttgaagt cgttcatgtc agccatcacg gacctccggg gggacggtcg
     1981 acgcgcgcgc gtcgggttga tcagtggcgg gtgcttcgcg ccttcttcgg tggcgggctc
     2041 gcttcgagac cgggcag
//
LOCUS       DATCCH010000480         5320 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_163362, whole genome shotgun sequence.
ACCESSION   DATCCH010000480 DATCCH010000000
VERSION     DATCCH010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5320
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163362"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1307)
                     /locus_tag="VIJ44_09240"
     CDS             complement(<1..1307)
                     /locus_tag="VIJ44_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014381102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEY5166123.1"
                     /translation="MSGPVTVGIDIGTSSVKAIAADEDGAVIASARIPHAFRVPTPGG
                     FEHDAADAWRRGPRQALGALGPLDVRGVSVAAMVPSLTAVDHDGIPVQPGLLYGDERG
                     RTETRGNPVETGELLTFLRWLVAAQPDAAGYWPAQAVANHELSGEAILDTSTAATAYP
                     LFDWKTWDPAVAAAVGARPDQFPRLVPTGWEAQRVDGNGPPLASGCIDALAEQLVAGA
                     DHDGDVLVLLGTTLIVWAVTTKTNDAPGLYLLPHTAPDKMLVGGPSNAGGLFCNWAAG
                     MLGAPSGPVDPRRVPVWVPYPRGERSPLDDPDRRAQLLDLDLTHDAASVRRAAFEASG
                     FVTRRILDIARDHHGITPRRLVASGGGTRVDDWVQALADATNLPVDCVAVPEGGALGS
                     AWLARIAAGLEEPTAMTDGRRWARVGRRFEPDRMWVDPVAERY"
     gene            complement(1304..2056)
                     /locus_tag="VIJ44_09245"
     CDS             complement(1304..2056)
                     /locus_tag="VIJ44_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007616889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEY5166124.1"
                     /translation="MALALALCDEADGLTMRHFGAHDLRVDTKPDNTPVTEADHAVEK
                     MIHERVEASRPGDAILGEEFGLVGESRRRWIVDPIDGTKSFVRGVPVWATLVALETDG
                     ELVVGAVSAPALRRRWWARRGGGAYMNGDPIRVSGVERLDDAHLCGPNERYFDEVSLG
                     DQYRSLARRSWRAVGFADFWGHALVAEGAVDAMIEPILGIWDIAALRPIVEEAGGRIS
                     DLTGDGWAEGAPCVTTNGKVHDEVLDLMRRTT"
     gene            complement(2145..3326)
                     /locus_tag="VIJ44_09250"
     CDS             complement(2145..3326)
                     /locus_tag="VIJ44_09250"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013426539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEY5166125.1"
                     /translation="MGDAVIVAAARTPIGTARRGSLASFSAFDLGRIAIEEALKRSGI
                     PADDLDDIVLGEVLQGGGDIARYVAVELGLTGVPGMAHNRHCASGMAAVQTAAANIRA
                     GMDTVVIAGGTESISQSPQVFRKLPGAYAGVQPWLSPSHPETPDAPTMDMSITVGWNT
                     AQRCNVTREEMDDWAYQSHQRAVAAIDDGRLREEIFAVEVPAGRGETRIFDTDEYPRR
                     DTTPEKLAKLAPLHPEIPGFSITAGNASGLNDGACAVVVCDGDYAAAHGLKPLAVIRS
                     WASAGVPPGDTGLGPTVAIPKALQRAGLTVDDISLVEINEAFASMAVASSRILGFAHD
                     IVNVNGSGCSLGHPVACTGARMITTLLYELGRRGGGYGVASMCAGGGMGSATVLEVPA
                     A"
     gene            3436..4968
                     /locus_tag="VIJ44_09255"
     CDS             3436..4968
                     /locus_tag="VIJ44_09255"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEY5166126.1"
                     /translation="MPDWGELQVDELRFMAGERPDAVAYRNLDAGTAITFGEWDRDSN
                     ALARGLVDLGVAKGDRVSVYLPSDGVLRWIVAYAAIHKAGAVVVPTNTRLTEPELASI
                     LRHAEVSAILTDETLLRSALAVRPDVASLRLAVSAGAIDGDDVVAWDQVRVDDMSAFQ
                     VDVDVSDLADVMYTSGTTGLPKGIAVRHGNLAMIPNIEPRWTGEGWIHGAPMFTFAGI
                     AFIYNPMKMGLVGLYQPRFDAGRWLEYVEHERPAFCMLVPAYAELIVAHPDFARRDLS
                     SLRQVSIGSAPLAPKTQLLLIERLPDATVGNSYGMTEAGPAYIVMPKEEISNRIGSVG
                     KPIGPMEIKIVGEGDVELAPREVGELLLRMRGKQREYYKNDEATAATWTTDGWLRSGD
                     LAYLDEDGFLYICGRKKDMIIRGGNNIYATDVEAVLLEHPDVREAAVTGVPHPVLGED
                     VAAWVVPRTDSELDRDQLLAFVAERLADYKRPRQLHLVDGLPRNATGKVMKHRLEVPP
                     AP"
     gene            5049..>5320
                     /locus_tag="VIJ44_09260"
     CDS             5049..>5320
                     /locus_tag="VIJ44_09260"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HEY5166127.1"
                     /translation="MALTELKGQTSGTQRVVIERGPVRVFAEALGDADDVYQRDEAPV
                     PPTFPFVMSYWGSLGSGGAAGLPIERLRGPGRAILHGEQAFEYHRWP"
BASE COUNT          792 a         1810 c         1892 g          826 t
ORIGIN      
        1 tcgtaccgtt ccgcgaccgg gtcgacccac atgcgatcgg gttcgaagcg gcgaccaacc
       61 cgggcccatc gtcggccgtc cgtcatcgcg gtcggctcct cgagcccggc ggcgatgcgg
      121 gccaaccatg ccgagccgag cgctccccct tctgggaccg cgacgcagtc gaccgggagg
      181 ttggtggcgt cggcgagagc ctggacccaa tcgtccacgc gggtcccacc accggaggcg
      241 acgagccgac gcggggtaat cccgtggtgg tcgcgggcga tgtcgaggat ccgtcgggtc
      301 acgaaccccg acgcctcgaa cgcggcgcgt cggaccgacg ccgcgtcgtg ggtgaggtcg
      361 aggtcgagga gctgggctcg acggtcggga tcgtcgagtg gggaccgctc accgcgcggg
      421 tacgggaccc agaccggtac ccgtcgagga tcgacgggtc cgctcggcgc gccgagcatc
      481 ccggcggccc agttgcagaa cagtccaccg gcgttgctgg ggccaccgac gagcatcttg
      541 tcgggcgcgg tgtgcgggag caggtacagg ccgggcgcgt cgttcgtctt ggtggtcacg
      601 gcccacacga tgagagtcgt cccgaggagc acgagcacgt cgccgtcgtg gtcggccccc
      661 gcgaccagct gctcggcgag ggcgtcgatg caccccgatg ccaggggcgg cccgttcccg
      721 tccacccgct gcgcttccca cccggttggc acgagccgtg ggaactggtc gggtcgggca
      781 ccgacggcgg ctgcgaccgc ggggtcccaa gtcttccagt cgaacagcgg ataggccgtc
      841 gcggcggtgg acgtgtcgag gatcgcttcg ccgctcagct catggttcgc gaccgcttgg
      901 gccggccagt acccggcggc gtcgggttgg gccgcgacga gccaccggag gaaggtgagc
      961 agctcaccgg tctcgaccgg gttgccacgg gtctcggtgc gaccccgctc gtcgccgtag
     1021 agcagaccgg gctggacagg gatgccgtcg tggtcgacgg ctgtcagtga cggcaccatc
     1081 gccgcgacgc tcaccccgcg cacgtcgagc ggcccgagtg ctcccagggc ctgccgggga
     1141 ccgcgtcgcc aggcgtcggc ggcgtcgtgt tcgaagccgc cgggcgtcgg gacgcggaac
     1201 gcgtgtggaa tccgggcgct ggcgatcacg gcgccgtcct cgtcagccgc gattgccttg
     1261 accgatgagg tcccgatgtc gatcccgacg gtgacgggcc cgctcacgtc gtccttcgca
     1321 tcaggtcgag gacctcgtcg tggaccttcc cgttcgtcgt gacgcagggc gcaccctcgg
     1381 cccagccgtc gccggtcagg tcactgatcc gcccgccggc ctcctcgacg atgggtcgca
     1441 acgctgcgat gtcccagatg ccgaggatcg gctcgatcat cgcgtccacg gcgccttcgg
     1501 cgacgagcgc gtggccccag aagtcggcga agccgaccgc tcgccaggag cggcgggcca
     1561 gcgaccggta ctggtctccg aggctcacct catcgaagta tcgctcgttc ggtccgcaga
     1621 gatgggcgtc gtcgagccgc tcgaccccag acacgcggat cgggtcgccg ttcatgtagg
     1681 cgccaccccc gcgtcgagcc caccaccgtc gtcggagcgc gggcgccgat accgcgccga
     1741 cgacgagctc cccgtcggtt tcgagggcca ccagcgtcgc ccagacgggc acaccccgga
     1801 cgaagctctt ggtgccgtcg atcgggtcga cgatccagcg gcgccgcgac tcgccgacga
     1861 gtccgaattc ctcaccgagg atcgcgtcgc ccggccgact ggcttcgacc cgttcgtgga
     1921 tcatcttctc gacggcgtgg tccgcctcgg tgaccggggt gttgtccggt ttggtgtcca
     1981 cgcgcaggtc gtgggcgccg aagtggcgca tggtgagccc gtcggcctcg tcacagagcg
     2041 cgagcgcgag cgccaagtcg gcttcgagat cggagctggt cagtggaagg tcctgcctcg
     2101 tctggggcgc agtgcgttct cgaggaggga acggcacgcc cggtctaagc ggccgggacc
     2161 tcgaggacgg tggccgaccc cattccacca ccggcgcaca tgctcgccac gccgtacccg
     2221 ccgcctcgtc gacccagctc gtagagcagc gtcgtgatca tgcgggcccc ggtgcacgcc
     2281 accgggtgcc cgagactgca gccgctcccg ttgacgttca cgatgtcgtg cgcgaagccc
     2341 aggatccggc tcgaggcgac ggccattgac gcgaaggcct cgttgatctc gacgagcgag
     2401 atgtcgtcga cggtgagtcc cgctcgctgc agcgccttcg ggatcgcgac ggtggggccc
     2461 agaccagtat cgccgggtgg cacgccagcc gacgcccagg accggatgac tgccaacggc
     2521 ttgaggccgt gcgccgcggc gtagtcaccg tcgcagacca cgacggcgca agcaccgtcg
     2581 ttgagtccgg acgcgttccc ggctgtgatg gagaagccgg ggatctcggg gtgcaacggg
     2641 gccagcttcg ccagcttctc tggcgtggtg tcgcgtcgcg ggtactcatc ggtgtcgaag
     2701 atccgggtct cgccgcgtcc cgccgggacc tcgaccgcga agatctcttc gcgcagacga
     2761 ccgtcgtcaa tggcggcaac ggcgcgttga tgggactggt acgcccagtc gtccatctcc
     2821 tcgcgggtca cgttgcaccg ctgggcggtg ttccagccga ccgtgatgga catgtccatc
     2881 gtcggggcgt cgggggtctc ggggtggctc ggggagagcc agggttggac gccggcgtac
     2941 gcaccgggaa gctttcggaa cacctgcggc gactggctga tgctctcggt gcccccggcg
     3001 atcacgacgg tgtccatgcc cgctcggatg ttcgccgccg cggtctggac ggccgccatc
     3061 ccggaggcgc agtgacggtt gtgggccatc ccggggacgc cagtgagacc gagctcgacc
     3121 gcgacgtagc gggcgatgtc gccgccgcct tggagcacct cgccgagcac gatgtcgtcg
     3181 agatcgtcag cggggatccc cgaccgtttc agcgcttcct cgatggcgat ccgaccgagg
     3241 tcgaaggcgc tgaaactcgc cagggagccg cgccgggcgg tcccaatcgg ggtgcgcgca
     3301 gcagccacga tgacggcgtc tcccacggtg atgctcctca tcttgtcggc cacgcgtcgc
     3361 gtggcgcggg ccacagcgga tgccgggcac cctaccctga cgttggcgtc aggtcgctgg
     3421 gctacgttcg cgctcatgcc ggattggggc gagctgcagg tcgatgagct gcgattcatg
     3481 gccggcgaac ggcccgacgc ggtcgcgtac cgaaacctcg acgcgggcac cgcgatcacc
     3541 ttcggcgagt gggatcggga ctcgaacgcg ctcgctcgcg gcctggtgga cctgggcgtg
     3601 gcgaaaggcg accgggtctc ggtctatctg ccgagcgacg gggtgctgcg ctggatcgtg
     3661 gcgtacgccg cgatccacaa ggccggggcg gtggtcgtcc cgacgaacac ccgcctgacc
     3721 gagcccgagc tggcctcgat cctccgccac gccgaggtgt cggccatcct caccgacgag
     3781 accttgctcc gttcggcgct ggcggtccgg cccgacgtcg cctccctccg attggcggtc
     3841 agcgccggtg cgatcgatgg ggacgacgtc gtcgcttggg accaggtccg ggtcgacgac
     3901 atgagcgcgt tccaggtcga cgtcgacgtg tccgacctgg cggacgtgat gtacacgtcg
     3961 ggaacgacgg ggcttcccaa aggcatcgcg gtgcgtcacg gcaacctcgc catgatcccg
     4021 aacatcgagc cgaggtggac cggcgaaggg tggattcacg gcgctccgat gttcaccttc
     4081 gccgggatcg cgttcatcta caacccgatg aagatggggc tggtcggctt gtatcagccc
     4141 cggttcgacg cgggacgctg gctcgaatac gtcgaacacg aacggccggc tttctgcatg
     4201 ctggtcccgg cgtacgccga gctgatcgtc gcccaccccg atttcgcccg tcgggacctg
     4261 tccagtctcc gacaggtgtc catcgggagc gctccgctgg cgccaaagac tcagctcttg
     4321 ttgatcgagc gactcccgga cgcgaccgtc gggaactcgt acggcatgac cgaggcggga
     4381 ccggcgtaca tcgtgatgcc gaaggaggag atcagcaatc ggatcgggtc ggtgggcaag
     4441 ccgatcggtc cgatggagat caagatcgtc ggtgagggcg acgtcgagct ggccccccga
     4501 gaagtcgggg agctcctgtt gcgtatgcgg ggcaagcaac gggagtacta caagaacgac
     4561 gaggccacgg cggccacgtg gaccaccgac ggctggctgc gcagcggcga tctcgcctac
     4621 ctcgacgaag acggcttcct ctatatctgc ggccgcaaga aggacatgat catccggggt
     4681 gggaacaaca tctacgccac tgatgtcgag gcagtcctcc tcgaacatcc ggacgtgcgc
     4741 gaggcggcgg tcactggcgt cccccacccg gtgctgggtg aagacgtcgc ggcgtgggtg
     4801 gtgccgcgca ccgacagcga gctcgaccgc gaccagttgc tcgccttcgt cgctgagcgt
     4861 ctcgccgact acaagcggcc gcggcagttg caccttgtcg atggcttgcc tcgcaacgcg
     4921 accggcaagg tcatgaagca ccgcctcgag gtgccgccgg caccctgacg tcggaccgaa
     4981 cctcggtccg ccccagcagg gccgcatctc ggcgcgagta gcgtcgccca tccgaaggag
     5041 gcagatcgtt ggcactcacc gagctcaagg gtcaaacgag cgggacgcag cgggtcgtga
     5101 tcgagcgcgg gccggtgcgg gtcttcgccg aggcgctcgg agacgccgac gacgtctatc
     5161 agcgtgacga ggcaccggtg ccgcccacgt tcccgttcgt catgtcgtac tggggatcac
     5221 tcggtagcgg cggcgcggcc gggctgccga tcgagcgtct gcgcggcccg ggccgcgcca
     5281 tcctgcacgg cgagcaggcc ttcgaatacc accgttggcc
//
LOCUS       DATCCH010000481         3086 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_163958, whole genome shotgun sequence.
ACCESSION   DATCCH010000481 DATCCH010000000
VERSION     DATCCH010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163958"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="VIJ44_09265"
     CDS             <1..774
                     /locus_tag="VIJ44_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020556439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY5166128.1"
                     /translation="VPSARMTGLGDGETRFRGRVALVTGAGQGVGAGIAAALAAAGAT
                     VAVNDLYADRAEAVAARISAEGGRAHACVADVTDLAAVEAMVGDVEAGVGPVDVLVNN
                     AGIPADGFVPRPFRAMPVADWERFVQLNLYGVLHGVKATVDGMCDRGWGRIVTVSSEA
                     GRMGLPIGISLYGASKAAAMGFSRHLAVELLGTGVTANCVALGLMATGPDDGRRPPSP
                     TTRLGDPVDVAAAVCFLASDEAEWITGQVLGVNGGALTT"
     gene            795..1148
                     /locus_tag="VIJ44_09270"
     CDS             795..1148
                     /locus_tag="VIJ44_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5166129.1"
                     /translation="MPKGIFIALSNATSDDVDDDFNEWYDAVHAREMLALPGVQSFRR
                     FRLAPAQVLPGDDVTKHRYLALYEVDVDDWEKFAAENQHAFVDGRITIRPELIELDPM
                     VLTMAFEEITPETSA"
     gene            complement(1158..>3086)
                     /locus_tag="VIJ44_09275"
     CDS             complement(1158..>3086)
                     /locus_tag="VIJ44_09275"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY5166130.1"
                     /translation="VPGAAVHIPANRTSVWWRHARVVQVIAATCLLWGGGYLVWRLGW
                     TGRGVNPVLFGALFAAEAYAWVSLALYAFLAWSVPTTTRPPLPDVVPTIDVFVCTYDE
                     PIAVVEATLTGSRAIRVPHRTYLLDDGDRPEMAALAFSLDAQHITRRDHSHAKAGNIN
                     HALGVTDSELILVLDADHVPRPDILDATVGYFRDGDVALVQTPHDFSNRDSVQHTKSQ
                     RHEQSLFYDVIAPGKDRHNAMFWCGSATLVRRDALVSVGGVLTDTVAEDFHTTIAMHA
                     RAWKTHYHAEALVQGRAPHDLAGFLLQRARWARGNLGVFRTSENPLTCRGLRPKQRLS
                     YFASLFNYFSGLQRLVLLSVLIVTLASGQLPMHASALTLVALWLPWSTSAFLTTIALG
                     RGALGPLDSTRYGLMTMGINVSGVLTLLRSRAGSFKVTPKEGTDDGGLQVLRRLGLLT
                     GVGVAMTAAWLARVLAVGGVLALPSMPSFATGVVIALGAWELACILKVLVPLIRRRQR
                     RTRYRLPVNLRARAAGTTVRVDVRDLTSAGLSFEMAAAVRVNTRLDLLTRLPDRVGGL
                     HDTVLPVEVRSCRRAAEPGMFRVGCEIRELAPDVRQLLNEFCEVVVSTAQLAGVLVPA
                     APLPGEPRPISWRDHRAS"
BASE COUNT          507 a         1028 c         1120 g          431 t
ORIGIN      
        1 gtcccgtcgg ccaggatgac aggcctgggc gacggagaga ctcgatttcg gggccgagtc
       61 gcgctcgtca ccggcgcggg acaaggtgtc ggggccggca tcgcggccgc gctcgccgcg
      121 gccggtgcga cggtggcggt caacgatctc tacgccgacc gggctgaggc ggtcgcggcg
      181 cggatcagcg ccgagggtgg tcgggcgcac gcgtgcgtgg ccgatgtgac cgacctcgcc
      241 gcggtggagg cgatggtcgg cgacgtcgag gccggcgtgg gaccggtgga cgtgctcgtg
      301 aacaacgccg gaatccctgc cgacgggttc gtcccgcgac ccttccgggc gatgccggtc
      361 gccgattggg agcggttcgt ccagttgaac ttgtacggcg ttctgcatgg cgtcaaggcg
      421 accgtggacg gcatgtgcga ccgggggtgg ggtcgcatcg tcacggtgtc gtccgaagcc
      481 ggtcggatgg gtctcccgat cggcatctcg ctgtacggcg cgagcaaggc cgccgcgatg
      541 ggcttctcgc gtcatcttgc cgtcgagctc ctcgggaccg gggtgacggc caactgcgtg
      601 gccctcggct tgatggccac cgggcccgac gacggccgcc gcccaccgtc gccgacgacc
      661 cggctcggag atcccgtcga cgtcgccgcc gcggtctgct tcctcgcttc cgacgaggcc
      721 gagtggatca ccggtcaggt gctcggcgtc aacggcgggg ccctcacgac ctgaaccgca
      781 aacaaggagc agctatgccg aaggggattt tcatcgcctt gtccaacgcc accagcgacg
      841 acgtcgacga cgacttcaac gaatggtacg acgcggtcca cgcccgcgag atgctggccc
      901 tccccggcgt ccaatccttc cgccgcttcc ggctcgcgcc ggcgcaggtc ctccccggcg
      961 acgacgtcac caagcaccgc taccttgcgc tctacgaggt cgacgtcgac gactgggaga
     1021 agttcgcggc cgagaaccag cacgcgttcg tcgacggtcg gatcacgatc cgtcccgagc
     1081 tgatcgaact cgacccgatg gtcctgacga tggccttcga ggagatcacc cccgagacct
     1141 cggcgtagcc ccgctcctca ggaagcccgg tggtcacgcc acgagatcgg ccgcggctcg
     1201 cccgggagtg gcgccgcggg aacgaggacc cccgccagct gcgcggtcga gacgaccacc
     1261 tcacagaact cgttgaggag ctggcggaca tcgggcgcga gctcgcgaat ctcacacccg
     1321 acgcggaaca tcccgggctc cgccgcccgg cgacaggagc gaacttcgac tggcaggacg
     1381 gtgtcgtgca gccccccgac acgatcggga agacgggtca ggaggtcgag tcgcgtgttc
     1441 actcggaccg ccgcggccat ctcgaaggac aggccggctg aggtcaggtc gcgcacgtcg
     1501 acgcgcaccg tcgttcccgc ggcacgagcg cgaaggttga ccggaagccg gtagcgggtg
     1561 cggcgctggc gacgccggat gagcggcacc aagaccttca ggatgcaagc cagctcccag
     1621 gcccccaggg cgatgaccac gccggtggcg aaggacggca tgctcgggag cgccagcacg
     1681 cccccgacgg cgaggacccg cgccagccac gcggccgtca tcgccacgcc gactccggtc
     1741 agcaacccca acctccggag cacctgcagg ccgccgtcgt cggtcccctc tttgggtgtg
     1801 accttgaacg agccggcacg cgaccggagc aacgtgagta cgccactcac gttgattccc
     1861 atcgtcatca gcccgtagcg cgtcgagtcg aggggtccga gcgcgccacg tcccagcgcg
     1921 atcgtggtca ggaacgccga cgtcgaccac gggagccaga gcgccaccag ggtcagggcc
     1981 gacgcgtgca tcgggagctg cccggacgcc agtgtgacga tcaggacgct caggagcacc
     2041 aggcgctgca ggccggagaa gtagttgaac agggacgcga agtagctcag gcgctgcttc
     2101 gggcgtagcc cgcggcacgt cagcggattc tcactggtgc ggaagacccc gaggttgccg
     2161 cgagcccatc gggcgcgctg cagcaggaac ccggcgagat cgtgcggggc gcggccctgg
     2221 acgagggcct cagcgtggta atgggtcttc caagcgcggg cgtgcatcgc gatggtggtg
     2281 tggaagtcct cggcgacggt gtcggtcaac acgccgccga ccgatacaag cgcgtcgcgg
     2341 cggaccaggg tcgccgagcc gcaccagaac atggcgttgt gccggtcctt gccgggagcg
     2401 atgacgtcat agaacagcga ctgttcatgc cgttgcgact tggtgtgctg caccgagtcg
     2461 cggttcgaga agtcatgcgg ggtctggacc aacgccacgt cgccgtcccg gaagtatccg
     2521 acggtggcgt cgaggatgtc cggccggggg acgtggtcgg cgtcgagcac gaggatcagc
     2581 tcactatcgg tgacacccaa cgcgtgattg atgttgcccg ctttggcgtg cgaatggtcg
     2641 cgtcgggtga tgtgctgggc gtcgaggctg aacgcgaggg cggccatctc gggccggtcg
     2701 ccgtcgtcga gcaggtaggt gcggtgcggg acgcgaatcg cccgggagcc ggtcagggtg
     2761 gcctcgacga ccgcgatcgg ctcgtcgtag gtgcagacga acacgtcgat cgtcgggacc
     2821 acgtcgggaa ggggcggtcg ggtcgtcgtc gggaccgacc aagccaagaa ggcgtacagg
     2881 gcgaggctga cccaggcgta ggcctcggcg gcgaagaggg ccccgaagag aacggggttg
     2941 acgccccggc cggtccagcc gagacgccag accaagtagc cgccgcccca caacagacag
     3001 gtcgcggcga tgacctgcac gacgcgggcg tgccgccacc agacactcgt tcggttggcc
     3061 gggatgtgca cggcggcgcc gggcac
//
LOCUS       DATCCH010000482         3274 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_164062, whole genome shotgun sequence.
ACCESSION   DATCCH010000482 DATCCH010000000
VERSION     DATCCH010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164062"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..821)
                     /locus_tag="VIJ44_09280"
     CDS             complement(96..821)
                     /locus_tag="VIJ44_09280"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY5166131.1"
                     /translation="MTEPLRRLWDDEAEAWAAWARAPDHDTFWHFTRWRLLEVLPEPG
                     GLTVDLGGGEGRLARELTTHGHRVVGIDSSETLTRLAVTHDEATAAAVGDLAALPVAT
                     ETADLAVACMSLQDVDDLDGAVREAARILRPGGRLAVAIVHPINSSGHFADDSASAPF
                     VIAGSYLSEFRYSDHLERDGLAMTFHSVHRPLEAYAQALNDAGLLIETIREPTWDSPT
                     GMSRFTGWDRIPIFCFIRAVKPR"
     gene            complement(818..1186)
                     /locus_tag="VIJ44_09285"
     CDS             complement(818..1186)
                     /locus_tag="VIJ44_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5166132.1"
                     /translation="MGQDITVTARRGAAPSVMTFDCNRSITGMAIERYASADDAHGSR
                     PPDVLARRLFDVGAWRVTIYSSVVTVEAPPERWEALEPKVEHAIRHLFGYYGDEAGWS
                     PDALRAIGVEPIKPPEPVAE"
     gene            complement(1205..1777)
                     /locus_tag="VIJ44_09290"
     CDS             complement(1205..1777)
                     /locus_tag="VIJ44_09290"
                     /inference="COORDINATES: protein motif:HMM:TIGR03086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03086 family metal-binding protein"
                     /protein_id="HEY5166133.1"
                     /translation="MSVNLPEVHARAVLDTTRAFIAGIGSDQWALPSNCADWDIRALA
                     NHLVGGNLWVAPLLDGQTIEDVGDRLNGDLLGADPLTAYDASAGQAAETFRAPGAMDR
                     MVAVSYGPVPGSVYCGHRFLDVLIHGWDLAESTGQDTNLPEDLVEACWEVVEPQREML
                     EGSGMFGTELSVPAGADRQSQLLAVLGRHE"
     gene            complement(1823..2479)
                     /locus_tag="VIJ44_09295"
     CDS             complement(1823..2479)
                     /locus_tag="VIJ44_09295"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEY5166134.1"
                     /translation="MTHPTLRLERRCWSAGDRVVVGIDEVGRGAWAGPVTVAAVVPAP
                     EHVRGIRDSKMLTRTERERAAIAVRGWAVAIGVGHASYEECDALGMTAALRTAANRAL
                     TELAEQGYEPDRIILDGKHDYLRRGDRVVTVVKGDQSVLAVAAASCVAKVTRDAMMRD
                     EAVHYPPYDFESNVGYPAPMHQHALRAYGPSAIHRRSWIFMDGLSWRGLPPPPGRLFA
                     "
     gene            2559..2768
                     /locus_tag="VIJ44_09300"
     CDS             2559..2768
                     /locus_tag="VIJ44_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5166135.1"
                     /translation="MQPGTRVEVRSRFDRRWARGFEVDAVVAEVDEPDPGYRIRRRSD
                     GAVLPSLFVSDEVREEKRGRSMWWM"
     gene            complement(2821..3111)
                     /locus_tag="VIJ44_09305"
     CDS             complement(2821..3111)
                     /locus_tag="VIJ44_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HEY5166136.1"
                     /translation="MGTYVMLTTLGPDGWATVRENPERIRQVTHEVESMGLKVVAQYA
                     LMGQWDFLNVIEAPDEATLAKAAITLAARGTMRTMTLQAVPIDELIERLNSD"
BASE COUNT          490 a         1069 c         1194 g          521 t
ORIGIN      
        1 gagccggtgg tcgcgaccaa ggcctagaac ccggcgtcgt tggtgcgtga tccgtcaccg
       61 ccaccgtctc ggctggcggg gcaacaacgg ccgccctacc gcggtttcac tgctcggatg
      121 aagcagaaga tcgggatgcg atcccaaccc gtgaaccgtg acatgccggt cgggctgtcc
      181 cacgtgggct ctcggatcgt ctcgatcagg aggccggcgt cgttgagggc ctgcgcgtag
      241 gcctcgagcg ggcgatgcac ggaatggaac gtcatcgcga gaccgtcacg ctcgagatga
      301 tcggagtacc ggaactccga caggtaggac ccggcgatga cgaacggggc gctggcgctg
      361 tcgtcggcga agtgtcccga cgagttgatg ggatggacga tggcgacggc gaggcgaccg
      421 ccgggacgca ggatccgggc cgcttctcga acggcaccgt cgaggtcgtc gacatcctgc
      481 aaggacatgc aggccacggc caggtcggcg gtctcggtgg cgaccggaag ggctgcgagg
      541 tcccccactg cggccgccgt cgcctcgtcg tgggtgaccg cgaggcgtgt cagggtttcc
      601 gagctgtcga ttccgacgac ccgatgaccg tgggtcgtca actcgcgggc caggcgaccc
      661 tcgccacctc cgaggtcgac ggtcagcccg cccggttcgg ggaggacctc gaggagccgc
      721 cagcgggtga agtgccagaa cgtgtcgtgg tccggggccc gagcccaggc ggcccacgcc
      781 tcggcctcgt cgtcccagag ccgacggagc ggctcggtca ctccgcgaca ggctcgggag
      841 gtttgatcgg ctcgacgccg atggcgcgca gcgcgtcggg tgaccagccg gcctcgtcgc
      901 cgtagtagcc gaagaggtga cggatcgcgt gctcgacctt gggttcgagc gcctcccacc
      961 gttcgggtgg ggcttcgacg gtcaccacgc tcgagtagat cgtcactcgc cacgcgccga
     1021 cgtcgaagag acgtcgcgcc agcacgtcgg gggggcgtga gccgtgcgcg tcatcggccg
     1081 acgcgtaccg ttcgattgcc atgccggtga tggaacggtt gcagtcgaag gtcatgacag
     1141 acggtgcggc gccgcgtcgc gccgtcacgg tgatgtcttg acccatgaag tcgagcctag
     1201 aacgtcactc gtggcggccg aggacggcga gcaactgcga ctggcggtcg gcgcccgctg
     1261 ggacgctgag ttcggtgccg aacatcccgc ttccttcgag catctcgcgt tgcggctcga
     1321 cgacttccca gcaggcctcg acgaggtctt cggggaggtt ggtgtcctgg ccggttgatt
     1381 cggcgaggtc ccatccgtgg atcaggacgt cgaggaagcg atgcccgcag tacaccgaac
     1441 cggggacggg tccgtacgac accgcgacca tgcggtccat cgctcccggg gcacggaagg
     1501 tttccgcggc ctgacccgcc gatgcgtcgt acgcggtgag ggggtcggcg ccgaggaggt
     1561 cgccgttcag ccggtctccg acgtcttcga tggtctgacc gtcaaggagg ggggccaccc
     1621 agaggttgcc gcccacgagg tggttcgcga gcgcccggat atcccagtcg gcgcagttcg
     1681 atggcagcgc ccactggtcg gacccaatcc cggcgatgaa ggcccgggtc gtgtcgagga
     1741 ccgcacgcgc atggacttcg ggcaagttga cgctcatgta ctcctccggg ggacgagggc
     1801 gccgcgtcgg atgggactga ccctatgcga agagccggcc cggaggcgga gggaggcccc
     1861 gccaggacag gccgtccatg aagatccagg accgccgatg gatcgcactg ggaccgtagg
     1921 cccggagcgc atgttggtgc atcggtgccg gatagccgac gttggattcg aagtcgtacg
     1981 ggggatagtg caccgcctcg tcgcgcatca tggcgtctcg cgtgaccttc gcgacgcagg
     2041 acgccgcggc gaccgcgagg accgactggt cgcccttgac gacggtcacg acccggtcac
     2101 cgcggcggag gtagtcgtgc tttccgtcca agatgatccg gtcaggctcg tagccctgtt
     2161 cggcgagctc ggtgagggcc cggttcgctg ccgtacgcag ggcagccgtc atcccgagcg
     2221 cgtcgcattc ttcgtacgag gcgtgtccca caccgatcgc aacggcccag ccgcgcacgg
     2281 cgatcgcggc gcgttcgcgc tcggttcggg tcagcatctt cgaatcgcgg atgccgcgca
     2341 cgtgctcggg ggcggggacc acggcagcga cggtcaccgg cccggcccag gcaccccgcc
     2401 cgacttcgtc gataccgacg accacccggt caccggcgct ccagcagcgg cgctcgagcc
     2461 ggagcgtggg gtgggtcacg acggcgaaac tatctgttca cgggctcgtc accgcggctc
     2521 cgttccgttt tcgtgagggg gtcggtagca tcagaggcat gcagcctggt acccgcgtcg
     2581 aggtccggtc ccggttcgac cggcgctggg cccggggctt cgaggtcgac gccgtcgtcg
     2641 ccgaggtgga cgaacccgat cccggctacc ggatccgtcg ccgatcggat ggggcggtcc
     2701 tgccgtcgct gttcgtcagc gacgaggtcc gggaggaaaa gcgcggtcgc tccatgtggt
     2761 ggatgtgacc ggcgcgtccc ggtccacccc gtgtggtgac gccaccgacg gcggcggcga
     2821 ctagtccgag ttgagccgct cgatcagctc gtcgatcggg acggcctgga gggtcatggt
     2881 ccgcatcgtg ccgcgggccg cgagcgtgat cgcggccttg gccagggtcg cctcgtcggg
     2941 ggcctcgatg acgttgagaa agtcccactg ccccatcagg gcgtactggg cgacgacctt
     3001 gagccccatc gactcgacct cgtgggtcac ctgccggatc cgctcggggt tctcgcggac
     3061 ggtggcccac ccgtcgggtc cgagcgtcgt gagcatcacg taggttccca cggcgcggag
     3121 cgtagagcgt cgccgcgagg cctgtcagga ctcgatgcgg gcgatgttct cggcctcgcc
     3181 gggatcgggc tcggtgctga accaggcgtc gagcatctcc cgggccaact cggcgctggt
     3241 caaccggagt cccatgacga cgatgttggc gtcg
//
LOCUS       DATCCH010000483         2833 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_164082, whole genome shotgun sequence.
ACCESSION   DATCCH010000483 DATCCH010000000
VERSION     DATCCH010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164082"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            236..1141
                     /gene="cysD"
                     /locus_tag="VIJ44_09310"
     CDS             236..1141
                     /gene="cysD"
                     /locus_tag="VIJ44_09310"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009739650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEY5166137.1"
                     /translation="MPTQLAHLDALEAESIHIIREVAAEFERPVLLFSGGKDSAVMLR
                     LAEKAFWPARIPFPVMHVDTGHNFDEAIDYRDRRVAELDLQLVVASVQASIDAGRVVE
                     EHGPRASRNRLQTVTLLDAITEHRYDAVFGGGRRDEEKARAKERVYSFRDEFGQWDPK
                     NQRPELWSLYNGRHRKGEHIRVFPLSNWTELDIWQYIATDGVEIPSIYYAHRRPVVRR
                     DGMLLAVTPYLGHLDGETPETLLVRFRTVGDATCTGAVESAAATVADVIAEVAASRVT
                     ERGETRADDRISEAGMEDRKREGYF"
     gene            1142..2398
                     /locus_tag="VIJ44_09315"
     CDS             1142..2398
                     /locus_tag="VIJ44_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014694721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY5166138.1"
                     /translation="MELLRFATAGSVDDGKSTLIGRLLLETKQIFEDQLEAVERTSRD
                     RGFDYVNLALLTDGLRAEREQGITIDVAYRYFATPRRKFIIADTPGHTQYTRNMVTGA
                     STADMALVLIDARKGVLEQSRRHAVIASLLQVRHLVLCVNKMDLVDFDESRFDEICAE
                     FTEFAAKLDIDDLAFIPVSALHGDNVVSHSPSTPWYEGPTLLHHLEHVYIGSDRNLID
                     ARFPVQYVIRPMSHDHHDYRGYAGQVASGMFRPGDEVLVLPSGFTTSIEAIDEYGGPV
                     DEAYPPMSVSVRLADDLDISRGDLLCRPNNAPTVTQDVDAMVCWFTDRPLREGAVYAV
                     KHTTRSARARVQRLQYRLDVNSLHRDEGQASLTMNDVGRVSLRTTTPLFVDEYRRNRV
                     TGSFILIDESNFETVGAGMILGASAP"
     gene            2410..>2833
                     /gene="cysC"
                     /locus_tag="VIJ44_09320"
     CDS             2410..>2833
                     /gene="cysC"
                     /locus_tag="VIJ44_09320"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020555338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEY5166139.1"
                     /translation="MRPDVVWHDEGISREDRWAARGVHGATVWLTGLSGSGKSTIAAA
                     LAGVLTGRGVLAYTLDGDNLRHGLNGDLGFSAADRAENIRRVAEVARLCADAGLVVLV
                     PVISPYRAGRDHARAIHDASDLVFVEVFVDAPLEVCETR"
BASE COUNT          453 a         1087 c          869 g          424 t
ORIGIN      
        1 gaccgtcgcc ctcgccggcc ggatcatcgc gtgcccgtgc tgcggctacg cccacgcgct
       61 gcgacccgtc ggccgccacg accgccgcct cgtcgtcgac tgcggcgacc ggaccctcgt
      121 gatgggggag ggatcccacc tctaccgccg gccccgggtc ctgatcccca cttactgaca
      181 ccggaccgcg ccgccgggtc gacccgccgg gcccatcggc caaactggtc ggtccatgcc
      241 gacccagctg gcccacctcg acgccctcga agccgagtcg atccacatca tccgtgaggt
      301 cgcggctgag ttcgaacgcc cggtcctgct gttctccggc ggcaaggact ccgcggtgat
      361 gctccgcctg gccgagaagg cgttctggcc ggcccggatc ccgttcccgg tcatgcacgt
      421 cgacaccggc cataacttcg acgaggcgat cgactaccgg gaccgccgag tcgccgagct
      481 cgatttgcag ctcgtcgtgg cgtcggtcca ggcctcgatc gatgccggcc gcgtcgtcga
      541 ggagcacggc ccgcgcgcct ctcggaaccg tctccagacc gtgacgctcc tcgacgccat
      601 caccgagcac cgctacgacg cggtcttcgg cggcgggcgc cgcgacgaag agaaggcccg
      661 agcgaaagag cgcgtgtact cgttccggga cgagttcggc cagtgggacc ccaagaacca
      721 gcgtcccgag ctgtggtcgc tctacaacgg ccggcaccgc aagggtgagc acatccgcgt
      781 cttccccctc tcgaactgga ccgagctcga catctggcag tacatcgcca ccgacggggt
      841 cgagatcccc tccatctact acgcgcaccg gcgccccgtc gtccgccgtg acggcatgct
      901 gctcgctgtc accccctacc tcgggcacct cgacggtgag acgcccgaga cgctgctggt
      961 ccggttccgc accgtcggcg atgccacctg caccggcgcc gtcgaatccg ccgccgcgac
     1021 cgtcgccgac gtcatcgccg aggtcgccgc ctcacgggtc acggagcggg gcgagacccg
     1081 agccgacgac cgcatctccg aagccggcat ggaggaccgc aagcgggagg gctacttcta
     1141 gatggagctc ctccgcttcg cgaccgccgg ctccgtcgat gacgggaaga gcaccctcat
     1201 cggccggctg ctcctcgaga cgaaacagat cttcgaagac cagctcgaag ccgtcgagcg
     1261 caccagccgc gaccgaggct tcgactacgt caacctggcg ttactcaccg acgggctccg
     1321 agccgaacgc gagcagggca tcaccatcga cgtcgcctac cggtacttcg cgaccccccg
     1381 acgcaagttc atcatcgccg acacccccgg acacacccag tacacccgca acatggtgac
     1441 cggcgcgtcg accgccgaca tggccctcgt cctcatcgac gcccgcaaag gggtgctgga
     1501 gcagtcccgc cgtcacgccg tgatcgcctc cctcctccag gtcaggcatc tcgtcttgtg
     1561 cgtcaacaag atggacctcg tcgacttcga cgagtcccgc ttcgacgaga tctgcgccga
     1621 gttcaccgag ttcgcggcca agctcgacat cgacgacctc gcgttcatcc ccgtctccgc
     1681 cctccatggc gacaacgtcg tgtcgcactc gccgagcacc ccctggtacg agggcccgac
     1741 gctgctccac catctcgaac acgtctacat cggctctgac cgcaacctca tcgacgctcg
     1801 tttccccgtc cagtacgtca tccggccgat gagccacgac caccacgact accgcggcta
     1861 cgcggggcag gtggcgagcg gcatgttccg acccggcgac gaggtgcttg tcctcccgag
     1921 cgggttcacg accagcatcg aagccatcga cgagtacggc ggcccggtcg acgaggcgta
     1981 tcccccgatg tcggtgagcg tccggctcgc tgatgacctc gacatcagcc gcggtgacct
     2041 gctgtgtcgc cccaacaacg cgccgacggt gactcaagac gtcgacgcca tggtctgctg
     2101 gttcaccgac cggcccctac gggaaggcgc ggtctacgcg gtgaagcaca ccacccgcag
     2161 cgcccgggcc cgagtgcagc gccttcagta ccgcctggac gtgaactcgc tacaccgcga
     2221 cgagggccag gcatcactga cgatgaacga cgtgggccgg gtcagcctgc gcaccacgac
     2281 cccgctgttc gtcgacgagt accgccgcaa ccgggtcaca ggctcgttca tcctcatcga
     2341 cgaatcgaat ttcgagaccg tcggcgccgg gatgatcctc ggcgcgtcgg cgccgtgagc
     2401 gtctcgccgg tgagacccga cgtcgtctgg cacgacgagg ggatctcccg ggaggaccgc
     2461 tgggcggccc gcggcgtgca cggcgccacc gtgtggctca ccggcctctc gggctccggg
     2521 aagtcgacga tcgccgccgc gcttgctggc gtgctcaccg gtcgaggagt cctcgcttac
     2581 accctcgacg gcgacaacct ccgccacggc ttgaacggcg acctcgggtt ctccgccgcg
     2641 gaccgggccg agaacatccg gcgtgtcgcc gaggtggccc gcctctgcgc cgacgccggc
     2701 ctcgtcgtcc tggtccccgt catcagcccc taccgggccg gacgggacca cgcccgcgcc
     2761 atccacgacg ccagcgacct ggtcttcgtc gaggtgttcg tcgacgcgcc tctcgaggtg
     2821 tgcgagaccc gcg
//
LOCUS       DATCCH010000484         2403 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_164436, whole genome shotgun sequence.
ACCESSION   DATCCH010000484 DATCCH010000000
VERSION     DATCCH010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164436"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..117)
                     /locus_tag="VIJ44_09325"
     CDS             complement(<1..117)
                     /locus_tag="VIJ44_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEY5166140.1"
                     /translation="MPNGARHHILSVLVENKFGVLSRIAGLFARRGFNIVSLA"
     gene            complement(126..1856)
                     /locus_tag="VIJ44_09330"
                     /pseudo
     CDS             complement(126..1856)
                     /locus_tag="VIJ44_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018725895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
     gene            complement(2060..>2403)
                     /locus_tag="VIJ44_09335"
     CDS             complement(2060..>2403)
                     /locus_tag="VIJ44_09335"
                     /inference="COORDINATES: protein motif:HMM:NF018533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formamidopyrimidine-DNA glycosylase"
                     /protein_id="HEY5166141.1"
                     /translation="LRAVVGKRRTAIKAALMNQAAIAGLGNLLVDEILWRAGIDPTRS
                     AADLDRTEQGRLARTISTTLRVLGRRGGSHTGDLMSARHPGGFCPRDGAPLRRDTVGG
                     RTTYWCPAHQR"
BASE COUNT          380 a          813 c          801 g          409 t
ORIGIN      
        1 cgccaacgac acgatgttga agccccgccg ggcgaagagg cccgcgatgc ggctgagcac
       61 accgaacttg ttctccacca ggacgctcag gatgtggtga cgcgctccat ttggcacagg
      121 ttctcctacc gtccgccgcg ccggtcgtgg cccgggtcca agatgatgtc gtcgttcgac
      181 cctcccgccg gaaccatcgg gtacaccttc tcccgatagt cgacgcggaa gtccacgacc
      241 accgggcggt cgtcgatctc gttcgccttc tcgatcgtgg cgacgacatc gttggggtga
      301 tcgacccgaa atccgacgca gcccatcgct tcggcccact tgacgtagtc ggggaggtcc
      361 ggtgacaggt acacctcgct gtagcgctcc tcgtagaaga gctcctgcca ctggcgaacc
      421 atgcccaggt acgcgttgtt gagaatggcg atcttgaccg ggatccgctc cgcgctggcg
      481 gtcacgagct cctgggccgt catctggaaa cagccgtctc catcgacggc ccacaccagc
      541 cgttcgggct tcccaacctt cgcgccgatc gccgccggca ccgcgaagcc catcgtcccg
      601 aggccgccag agttgaccca ggtatacgga aagttgaacc gccaccgctg gctggtccac
      661 atctggtgct ggccgacccc ggacaccacg atcgtgtcgt ccggagtgtg gtcacgcagc
      721 atgtcgatca cgaactgcgg cttgagtggt ccgtcctcgt gctggtccag gaccaacggg
      781 tagtgctcct gccattcgcg cagctgggta tgccacggct ccagtgacgg ccacgaccgc
      841 ttggcccggt cggcgcgcac cgcctgggtc aactcctcga tgaccagtcg gcagtccccc
      901 acgatgggca cgtccggacg ccggaccttg ccgagctcgg cggggtcgat gtcgacgtgg
      961 atgatcttcg cgtcgggcgc gaaagcgccc accttgccgg tgacccgatc gtcgaagcgt
     1021 gatccgagcg ccacgaggag gtcagcgcgc tgcatcgagg tgacagcggt gtagttgccg
     1081 tgcatgccag gcatgccgag gcacagctcg tggtcgtcgg ggaacgcgcc gcgggccatg
     1141 agcgtcgtca ccaccgggaa accggtgagc tccaccagtt cccggagcgc ctcggcggcc
     1201 cgggccttga ggatcccacc gccggcatag atcacggggc gttgcgcgtc ggccatcagc
     1261 cgcgccgcgt ccttgatctg cttggagtgt ccccgcatcg tcggcttgta gccgggcagg
     1321 tccaccgtct cgggccagta ccactccatc atctggttcg agatgtcctt cgggacgtcc
     1381 accagcacag gtccgggccg cccagtcgtg gcgacgtgga aggcctcccg cacgatccgg
     1441 gggatgtcgc tcgcatcggt gacgagccag ttgtgcttgg tgaccggcat cgtgatcccg
     1501 accgtgtcgc actcttgaaa cgcgtcggtg ccgatcaccg cgtaggcgac ctgaccggtg
     1561 atcacgacga tgggcaccga atccatatac gcgtcgcaga gcggcgtgac gatattggtc
     1621 gcgcctggac cgctggtcac catcgccacg ccgggccggc cggtggcgtc ggcgtacccc
     1681 tcagccatgt ggccggcgcc ctgctcgtgg cggacgagga tgtgacggat cgaactgtca
     1741 atcagcgggt cgtacaccgg caggatcgct ccgcccggga ggccgaacat gacctcggtg
     1801 ccttccatct cgagactctt gatgagggct tgcgccccgg tcagctgcat tgcaggtcct
     1861 tgccttggtc gggatacgaa acacggctcg ggatatgaaa cgcccctcgc tttggcgagg
     1921 ggcgggagcg gcgcacgcgc gcgtggaggc gggcgtgcgc tacgtaacta cgaggatctg
     1981 gtcgcggctc atgaccaggc cattgtgcca gacgcgacgc cgctcggcaa ccgcgtactg
     2041 accacgaacc ggctgagcgt cagcgctggt gtgccgggca ccagtaggtg gtgcgaccac
     2101 ccacggtgtc ccggcgcagc ggcgcaccgt cgcgagggca gaagccgccg gggtggcggg
     2161 ccgacatgag atcgccggtg tgagacccgc ctcgtcgccc caagacccgc agcgtcgtgc
     2221 tgatcgtccg ggcgaggcgt ccttgctcgg ttcggtcgag gtccgcggcg gaccgggtgg
     2281 ggtcgatccc ggcccgccag aggatctcat cgacgaggag gttgccaagc ccggcgatcg
     2341 cggcctggtt catcaaggcg gccttgatcg cggttcgacg cttcccgacc acggctcgca
     2401 gtc
//
LOCUS       DATCCH010000485         5651 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_38391, whole genome shotgun sequence.
ACCESSION   DATCCH010000485 DATCCH010000000
VERSION     DATCCH010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5651
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38391"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="VIJ44_09340"
     CDS             complement(<1..804)
                     /locus_tag="VIJ44_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5166142.1"
                     /translation="MPPRWSVLCLVALLAGLVAVPLVEAPASAQTDPAVAVALSALQQ
                     AQSAAHVAADQVMATTQQQAAVQAKIADNQARITQLEAQIPLLRAQEAQLRRVLQQRA
                     AALYASSGPLGGYDTLIPDPSLTAMRRQVLANAAAKRDHQNADQLAALQTQLAQAEAD
                     VKTQQTALAQQSAQLNQLAAQLQAQQALMNQRVADANSALERAKELGVLRAAGREPIQ
                     GPSVLTAAQMAGWIRSRGYNPHIETTIDDLAQIYIQEGNDEGVRGDFAFA"
     gene            909..1799
                     /locus_tag="VIJ44_09345"
     CDS             909..1799
                     /locus_tag="VIJ44_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012889335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03620 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEY5166143.1"
                     /translation="MSAELGRIGVWTFVLDRLPMGQARELAAELEELGFGAIWVPEAL
                     GRDPLVNAVLLLAGTRECVVATGIASIYARSPSAMASGWTTVSEAFPDRFLLGLGVSH
                     EPMVKGIHHQTYGPPVETMHGYLDAMDATPSFAAEPTAAPERVLAALGPRMLALAAAA
                     TRGAHPYLVPPEHTVRARATLGSGPLLAPEQMAVLETDPHRARAVARDSLRIYLPGLP
                     NYVNNLRRLGFTDEDVAAPLSDRLVDAIVAWGDEEAIRVRVQAHLDAGADHVCVQVLP
                     GDDPERVAADYRRLAPALMP"
     gene            1796..2287
                     /locus_tag="VIJ44_09350"
     CDS             1796..2287
                     /locus_tag="VIJ44_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5166144.1"
                     /translation="MTESPIRAVRRVVVASDDDGRAGVVVDGPSPHVHTLPGMPADLG
                     LIDLWYTDGTPRDRTSVDPADRELSVAPVQGGTLFRVVQLPPDGELPHDEHGAPQLFW
                     HETSTVDYSLLLEGTLWLLTGHDEIELHPFDAIVVRGGRHAWSNRADTPARLVTVGVG
                     LPD"
     gene            complement(2299..3495)
                     /locus_tag="VIJ44_09355"
     CDS             complement(2299..3495)
                     /locus_tag="VIJ44_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014143091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY5166145.1"
                     /translation="MAEFPKIISVDDHVVEPPGVWQDRLPAKYRDVGPRTMRAPLGEM
                     TFVGGKFSYSPGTDGPMCDWWLYEDLVYPLTRLSAAVGFDRDEVKVTAITFDEMRPGC
                     FDPKARLEDMDANWTESSLSFPTFPRFCGQTFLEAKDRDLALLCVQAYNDWMFDEWCG
                     PSGGRLIPLPLIPLWDARLAADEVHRSAARGGHAVCFTEIPPFLGLPSVHDQDGYWDP
                     FFAACAETDTVVCMHIGSSSKMPSTSPDAPAGVGSTLTYMNAAMSMVDYLMSGVFETH
                     PALKIAYSEGQIGWIPYILERADAVWEHNRAWAGLEGRVRQPPSQYYREHVYGCFFDD
                     AHGLQSLEQVGADNITFETDYPHSDSTWPHTREIAEKLTKNLDDETIYKIMRANAIRM
                     LSLDVT"
     gene            complement(3537..4358)
                     /locus_tag="VIJ44_09360"
     CDS             complement(3537..4358)
                     /locus_tag="VIJ44_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5166146.1"
                     /translation="MRTLLAFLLSVVVVVLVPFADLGLWVQRELVSTSSFVRLGEDVL
                     SQAPVQAALGQRIVAELEQRVPGLAAQDSTVRSIVDQALRDPALGSAFDNALASTHDQ
                     LRSGLDPLELDLNPVLPLVRQQLPAALAARVPVSATLNPVIVLRRRDAPVLWEGVQLV
                     QGGAAAVPLLALALLAAAVLVARRRGGLCIALGAFITVISLGVLGLVKPGRSLLEHQV
                     GDPTQRAAFGSGYDTVLRSFVQQTIVMALAGVVLAVVGGVLVWQHDRNRRPTGWA"
     gene            complement(4861..5538)
                     /locus_tag="VIJ44_09365"
     CDS             complement(4861..5538)
                     /locus_tag="VIJ44_09365"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY5166147.1"
                     /translation="MEGLVAGYGEVEVLHGVDLAVSPGTVVALLGANGAGKSTLCAVA
                     SGLLAPTAGRVCLEGRDVSDLSADERARAGLLLVPESRGIFPGLSVEENLRVFLRSDP
                     ERKTAYDRFPILFERRRQTAGLLSGGEQQMLSLAPALARPPKVFIADEPTLGLAPLAG
                     REVIRALLELRELGVAILLVEEKAREVMQVADSVAFMALGRVVWFGSREDADEERLAA
                     VYLGTNL"
BASE COUNT          884 a         1986 c         1950 g          831 t
ORIGIN      
        1 cgcgaacgcg aagtcgccgc gaacgccttc gtcgttgccc tcttggatgt agatctgggc
       61 gaggtcgtcg atcgtggtct cgatgtgcgg gttgtacccg cgggaccgga tccagccggc
      121 catctgcgcc gcggtgagca ccgaggggcc ttggatcggt tcgcgccccg cggcgcgcag
      181 gacgccgagc tccttggctc gttcgagggc cgagttggcg tccgccacgc gttggttcat
      241 cagcgcctgt tgcgcttgga gctgcgccgc gagttggttc agctgcgcgc tttgctgcgc
      301 gagggcggtc tgttgggtct tgacgtcggc ttcggcctgg gcgagctggg tttgcaacgc
      361 ggcgagctga tcggcgttct gatggtcccg tttcgcggcc gcgttcgcca gcacctggcg
      421 tcgcattgcg gtgagcgacg ggtcggggat cagcgtgtcg tagccaccga gcggtcccga
      481 cgaggcgtac aacgccgcgg cgcgctgttg gaggacacga cgcagctggg cctcttgagc
      541 ccggagcagc gggatctggg cctcgagctg ggtgatccgg gcctgattgt cggcgatctt
      601 ggcttggacc gcggcttgct gctgggtggt ggccatcacc tggtccgcgg cgacatgcgc
      661 cgcggactgg gcctgctgga gcgcgctgag cgccacagcc acggcgggat cggtctgcgc
      721 cgaggcgggt gcttcgacga gcggaaccgc gaccaggccg gccaggagcg cgaccaggca
      781 gaggaccgac cagcggggag gcacgaaccg ataggttagg gaatcaggaa ccacaccagc
      841 aacagtggcc tcagtcccgc accagggaag ggccctacgc taccggccat ggtcccgggt
      901 cccgtgagat gagcgccgag ctgggtcgga tcggggtctg gaccttcgtc ctggatcgcc
      961 tcccgatggg acaggcccgg gagctggccg ccgagctaga agagctcggg ttcggggcga
     1021 tctgggtccc ggaggccctg gggcgggacc cactcgtcaa cgcggtgctg ctgcttgccg
     1081 gaacccggga gtgcgtggtg gcgaccggga tcgccagcat ctatgcacgc agtccgtccg
     1141 cgatggcgtc gggctggacg acggtcagcg aggccttccc cgaccggttc ctcctcgggc
     1201 tgggggtgag ccacgagccc atggtgaagg gcatccacca ccagacgtac gggccaccgg
     1261 ttgagaccat gcacggctac ctcgacgcga tggacgccac cccgtcgttc gccgccgagc
     1321 cgaccgcggc gccggaacgg gtcctcgccg cgctcggccc ccggatgctg gccctcgccg
     1381 cggcggcgac tcgcggtgcg cacccgtact tggtgccgcc cgaacacacc gtccgggccc
     1441 gggccacgct cgggtctggg ccgttgctcg ccccggagca gatggcggtg ctcgagaccg
     1501 acccgcatcg ggcgcgggcc gtcgcccgcg acagcctgcg catctacctt ccgggcctcc
     1561 ccaactacgt caacaacctg cgtcggctcg gcttcaccga cgaggacgtc gccgcgccgc
     1621 tcagcgatcg gctggtcgac gccatcgtcg cctggggcga cgaggaagcc atccgcgtcc
     1681 gagtccaggc gcacctcgac gccggcgccg accacgtgtg cgtccaggtc ctccccggag
     1741 acgatcccga gcgagtcgcc gccgactacc ggcgcctcgc gccggccctg atgccgtgac
     1801 cgagagcccg atccgtgccg tccggcgcgt cgtcgtcgcg agcgacgacg acggtcgagc
     1861 gggtgtcgtc gtcgacgggc cgagcccgca cgtccacacc cttcccggca tgcccgccga
     1921 tcttggtctc attgacctct ggtacaccga cggcacgccc cgcgaccgca cgagcgtcga
     1981 ccccgcggac cgggagctca gtgtcgctcc cgtccaaggc ggcacgctgt tccgcgtggt
     2041 ccagttgcca cccgacggcg agctccccca cgatgagcac ggcgccccac agctgttctg
     2101 gcacgagacg tcaacggtcg attacagcct gctcctcgaa ggaacgctat ggctgctgac
     2161 cggccacgac gagatcgagc tgcacccctt cgacgcgatc gtcgtgcgcg gtggtcgcca
     2221 cgcctggagc aaccgcgcgg acaccccggc ccgtctcgtc acggtgggcg tcggcctccc
     2281 cgactgacca gccccggatc aggtgacgtc gagggaaagc atccggatcg cgttggcccg
     2341 catgatcttg tagatcgtct cgtcgtcgag gttcttcgtg agcttctccg cgatctcgcg
     2401 ggtgtgcggc cacgtcgaat ccgagtgcgg gtagtcggtc tcgaaggtga tgttgtccgc
     2461 gccgacctgc tcgagggact gcagcccgtg cgcgtcatcg aagaagcatc cgtacacgtg
     2521 ctcgcggtaa tactggctcg gcggctggcg gacccgaccc tcaagtccgg cccaggcccg
     2581 gttgtgctcc cagaccgcat cggcccgctc gaggatgtag ggaatccagc cgatctggcc
     2641 ttcggagtac gcgatcttca gcgccgggtg cgtctcgaac accccggaca tcaggtagtc
     2701 gaccatcgac atcgcggcat tcatgtatgt gagcgtggat ccgacccccg ccggcgcgtc
     2761 gggcgacgtt gacggcatct tcgacgacga gccgatgtgc atgcagacca cggtgtcggt
     2821 ctcggcgcac gcggcgaaga acggatccca gtagccgtct tggtcgtgga ccgacgggag
     2881 gccgaggaac ggcgggatct ccgtgaagca caccgcgtgg cccccacgcg ccgcactgcg
     2941 gtggacctcg tccgcggcca accgcgcatc ccagaggggg atcaacggca gcgggatgag
     3001 acgtcccccg gacggcccgc accactcgtc gaacatccag tcgttgtagg cctgcacgca
     3061 gagcagcgcg agatcccggt ctttggcttc gaggaacgtc tggccgcaga agcgtgggaa
     3121 ggtcgggaag gagaggctcg actcggtcca gttggcgtcc atgtcctcga gccgggcctt
     3181 cgggtcgaag catcccggtc gcatctcgtc gaaggtgatc gcggtgacct tgacctcgtc
     3241 gcggtcgaag cccaccgcgg ccgacaagcg agtcagcggg tacaccaggt cttcgtagag
     3301 ccaccagtcg cacatcgggc cgtcggtgcc cggcgagtag ctgaacttgc cccccacgaa
     3361 cgtcatctca ccaagcgggg ctcgcatggt gcgcggtccc acgtctcggt acttggcggg
     3421 cagtcggtcc tgccacaccc ccggtggctc gaccacgtgg tcgtcgacgg agatgatctt
     3481 cgggaactcg gccatgcggc gagcataggc ctgacggtcc gtcagctcag ggggtgtcac
     3541 gcccagccgg ttggtcgccg gttccgatcg tgctgccaca ccagcactcc cccgaccacc
     3601 gcgaggacga cgccggcgag ggccatcacg atggtttgct gcacgaacga tcggagcacg
     3661 gtgtcgtagc cggagccgaa cgcggctcgt tgcgtggggt caccgacctg gtgctcaagt
     3721 agcgaccggc cgggtttgac caacccgagg accccgaggg agatcaccgt gatgaacgcg
     3781 ccgagggcga tgcacagccc accccggcga cgcgctacga gcacagccgc ggcgagcagc
     3841 gcgagcgcca ggagcggcac cgccgcggcc ccgccctgga cgagctgcac cccctcccac
     3901 agcaccggcg cgtcgcggcg gcgcagcacg atcaccgggt tgagcgtcgc gctgaccggc
     3961 acccgggcgg cgagcgccgc gggcagctgc tggcggacga gcggcaacac cgggttcaga
     4021 tcgagctcga gggggtcaag gccgctgcgg agctggtcgt gcgtgctcgc cagagcgttg
     4081 tcgaacgccg acccgagcgc ggggtcgcgc aaggcctgat caacgatcga ccgcactgtc
     4141 gagtcttggg ccgcgagccc ggggacccgc tgctccagct cggcgacgat ccgttgcccg
     4201 agcgcagcct gcactggggc ctgggacaag acgtcttcac cgaggcgcac gaacgatgac
     4261 gtgctgacga gttcccgttg cacccacagc ccgaggtcgg cgaagggcac gagcaccacg
     4321 acgacgaccg aaaggaggaa ggccaggagc gtgcgcatag gcgccgttag tgtggccgcc
     4381 cgcgccggcc gggagacatc atgccgctcc cgcgccccca cccgatggct ccagcccgct
     4441 gcccggcgga ggctcgcggg ccaggtcatc gtcgtcatgg gcggcaggac ccggctgagc
     4501 acggcgatgg catcggcgtt cgcccgcctg ggcgaccggg atcatcaccc tcgatcgtcc
     4561 ggacaagctc acgcacgcgc cgacgagctc cgattcgacg accgggtccg gctggtcctg
     4621 cgccatcaga tgcacagtca gtttctggtc cgagcgcggg ctggccgaag cgaagcgctt
     4681 cgtcgcgatg agctcggcgc ccggcctcgt atccggcgac aggaccgagg ggtaccaggc
     4741 agtggccgag cgtcgaccag tgcaattcaa aggcaagtga ggcgctcggc ccgcccgacc
     4801 accaccccgg ctgaagaccc cggctaaagg tcgccggacc ggtcgaggac agattcgccc
     4861 ttacaggttc gtgccgaggt acaccgccgc gagtcgctct tcgtcggcgt cttcacggga
     4921 cccgaaccag accacacgcc cgagcgccat gaaggcgacg ctgtcggcaa cctgcatgac
     4981 ctcgcgcgcc ttctcctcaa ccagcaggat ggccacaccg agctcgcgca gttccagcaa
     5041 agcgcgaatc acctcccgtc ccgcgagcgg tgccaagccg agcgtcggct cgtccgcgat
     5101 gaacaccttc ggcggccgcg ccaacgccgg cgcgagactg agcatctgct gctcaccccc
     5161 ggacaggagc cctgcagtct ggcgtcgccg ctcgaaaaga atcgggaagc gatcgtaagc
     5221 cgtcttgcgc tcggggtccg agcggaggaa cacccggagg ttttcctcga cgctcaggcc
     5281 agggaagata ccccgcgatt cgggaaccag cagcagccca gctcgagcgc gctcgtcggc
     5341 cgacaggtca ctgacgtcgc ggccctcgag acaaacgcgg cccgcggtcg gcgcgaggag
     5401 ccccgaggcg acggcgcaca gcgtcgactt cccggccccg ttcgcgccca ggagcgcgac
     5461 gaccgtcccc ggcgacaccg cgaggtcgac gccgtgcaat acctcgacct cgccatagcc
     5521 cgcgacgagc ccttccaccg cgagtgcagc gttcggtacc gcgagcgcct ctccatcggt
     5581 gggcgcgtcg agtaacggtg ccggtcccgc cgcagcacca cccgtggtcg tggtcccttc
     5641 acgcttgagc g
//
LOCUS       DATCCH010000486         3002 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_413542, whole genome shotgun sequence.
ACCESSION   DATCCH010000486 DATCCH010000000
VERSION     DATCCH010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413542"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="VIJ44_09370"
     CDS             complement(<1..431)
                     /locus_tag="VIJ44_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HEY5166148.1"
                     /translation="MTTLPGAALAAAWRRDLADRTAVPLDAAGCAAPSAPVLDTTIAH
                     LRREAAIGGYAAEAEARPTLAALRTSLGALVDLPGECVALAPNATTAFTAMLGAWPLR
                     PGARIGTLPSEYGSNRLALDALAGRRDWTLVELGTDGAGRL"
     gene            complement(428..1408)
                     /gene="egtD"
                     /locus_tag="VIJ44_09375"
     CDS             complement(428..1408)
                     /gene="egtD"
                     /locus_tag="VIJ44_09375"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017594548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HEY5166149.1"
                     /translation="MTTSPLDVHVDVHLSDATIADALAADVHAGLTRSPKELPPKWFY
                     DDRGSQLFDEITRLSEYYPTRAERSILTARAAEIADVTGADTLVELGSGTSEKTQLLL
                     GALAEAGTLRRFVPFDVSEATLRTAAQAVSEEYGVTVHAVVGDFERHLGALPEGGVRL
                     VAFLGSTIGNLAPAPRAAFLADVAGRLRPGDTLLLGTDLVKAVDRLEAAYNDAAGVTA
                     EFNRNVLRVINRALGADFVPERFDHVARWVPAASWVEMRLRARGAQRVRVADLDLAVG
                     FADGEELRTEISAKFTPDQVERELARAGFALQRWWTDSAGDFGVSLATRT"
     gene            complement(1416..2153)
                     /locus_tag="VIJ44_09380"
     CDS             complement(1416..2153)
                     /locus_tag="VIJ44_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5166150.1"
                     /translation="MCRHLAYLGPPIALTELLRDAPHGLVHQAQHPLHQTSGTANPDG
                     YGIAWYEAGAPEPRRRRSTTPIWADPELMTVARTVSSPAVLGAARLASPGSPVEVSGN
                     APFLAERFAFSLNGIVEGWHDGIGTELRQLVSPRRGAAIDGVTDGETLFGLALDLLDD
                     GAPFADALAGVVAAVEALTTGRLNLLLTDGATMAATACGNSLFGRQRDGAALIASEPI
                     DADAAWSPVPDRTVVVATATAMTEAPL"
     gene            complement(2159..>3002)
                     /locus_tag="VIJ44_09385"
     CDS             complement(2159..>3002)
                     /locus_tag="VIJ44_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017579537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEY5166151.1"
                     /translation="PHQTSRGRRPCVAAPDCSGSPEVHLPGGPFVMGTDTEPWAYDNE
                     RPAHLVDLPPFRIDRTPVTNGAYRAFVDDGGYDEPSWWSTAGWAWRNEAQLVAPEGWR
                     REGASTWSVRQFGCRADLAPGEPVQHACWYEADAFARWADKRLPTEAEWEYAASWSAK
                     GTKRRYPWGDRDPTPTLANLARLQLGPSDVRAHPGGATAAGVLAMVGDVWEWTSTTFA
                     GYPNFAAFPYREYSEVFFGDEYRVLRGGSWATHPSVCRTTFRNWDFPIRRQIFCGFRC
                     AGDA"
BASE COUNT          418 a         1017 c         1164 g          403 t
ORIGIN      
        1 tcgaggcgac ccgcgccgtc ggtgccgagc tcgacgagcg tccagtctcg acgtccggcg
       61 agggcgtcga gcgccaggcg gttgctgccg tactccgacg ggagggtacc gatccgagcc
      121 cccggtcgga gcggccacgc accgagcatc gccgtgaacg ccgtggtggc gttcggcgcg
      181 agcgcgacgc actcgccggg gaggtcgacg agggccccga ggctggtccg gagcgccgcg
      241 agggtgggac gcgcttcggc ctcggccgcg tagccgccga tcgcggcttc gcgtcgcaga
      301 tgggcgatcg tggtgtcgag gaccggtgcc gacggggcag cgcagccggc cgcgtcgagc
      361 ggcaccgcgg ttcggtcggc gaggtcgcga cgccacgccg cggcgagcgc cgcgccaggg
      421 agggtcgtca cgtgcgggtg gcgagcgaga caccgaagtc gccggccgag tcggtccacc
      481 agcgttggag cgcgaagccg gcccgggcca gctcccgttc gacctgatcg ggcgtgaact
      541 tggcgctgat ctcggtgcgg agctcctcgc cgtcggcgaa cccgacggcg aggtcgagat
      601 ctgccacccg cacccgttgc gcgccgcggg cgcggaggcg catctcgacc cacgacgccg
      661 ccggaaccca ccgggcgaca tggtcgaagc gttcgggtac gaaatcggcg ccgagcgcac
      721 ggttgatcac ccgcagcacg ttgcggttga actcggcggt cacgccggcc gcgtcgttgt
      781 aggccgcctc gagccggtcc accgccttga cgaggtcggt gccgagcagg agcgtgtcac
      841 cgggacgaag gcggccggcg acgtcggcca agaacgcggc gcgaggcgcg ggcgccaagt
      901 tcccgatcgt gctgccgagg aacgcgacga ggcggacgcc gccctcgggc agggccccga
      961 ggtggcgttc gaagtcgccc accacggcat ggacggtgac gccgtactcc tcgctgacgg
     1021 cctgggccgc ggttcgcagc gtggcttcgc tcacgtcgaa cggcacgaag cggcgcagcg
     1081 tgcccgcttc ggccagggca ccgagcagca gctgggtctt ctcggacgtg cccgagccca
     1141 gctcgacgag cgtgtcggcc ccggtgacgt cggcgatctc cgcggcgcgg gccgtgagga
     1201 tcgaccgttc ggcccgggtc gggtagtact cggagaggcg ggtgatctcg tcgaagagct
     1261 gggagccgcg gtcgtcgtag aaccacttcg ggggcagctc cttgggcgac cgggtcagcc
     1321 ccgcgtggac gtccgcggcg agcgcgtcgg cgatcgtggc gtcggagaga tggacgtcga
     1381 cgtggacgtc gagcgggctc gtggtcatgg cttcctcaca ggggggcctc ggtcatggcg
     1441 gtggcggtgg ccacgacgac ggtccggtcc gggaccgggg accaggctgc gtcggcgtcg
     1501 atgggttcgg acgcgatcag ggccgcgccg tcgcgttggc gcccgaacag ggagttgccg
     1561 cacgcggtgg cggccatcgt ggcgccatcg gtgagcaaca ggttcaggcg ccccgtggtc
     1621 agcgcctcca cggcggccac gacaccggcg agcgcgtccg caaatggggc gccatcgtcg
     1681 aggagatcca aagccaggcc gaacagtgtc tcgccgtcgg tgacaccgtc gatggcggcg
     1741 ccccgccgcg gggagacgag ctggcgcagc tcggtgccga tcccgtcgtg ccagccctcg
     1801 acgatcccgt tgagcgagaa cgcgaagcgc tccgcgagga acggtgcgtt ccccgacacc
     1861 tcgaccggag accccgggga ggccagccgg gccgcgccga gaaccgcggg ggaggagacg
     1921 gtgcgggcga cggtcatgag ctcggggtcg gcccagatcg gtgtcgtcga ccgccgccgc
     1981 cgaggttcgg gggcacccgc ctcgtaccag gcgatgccgt acccgtcggg gttggcggtc
     2041 ccgctggtct ggtgaagcgg gtgctgggcc tggtggacga gaccgtgggg ggcgtcgcga
     2101 agcagttcgg tgagcgcgat cggcggtccg aggtaggcga gatggcgaca catggcgttc
     2161 aggcgtcgcc ggcgcagcgg aagccgcaga agatctggcg tcgaatcggg aagtcccagt
     2221 tgcgaaacgt cgtgcggcag acgctcgggt gcgtcgccca cgatccgccg cgcaagaccc
     2281 ggtactcgtc accgaagaac acctcggagt actcgcggta cgggaacgcg gcgaagttcg
     2341 ggtagccggc gaaggtcgtg gacgtccatt cccagacgtc gccgaccatg gccagcacgc
     2401 ccgccgccgt ggcgccgccc gggtgggcgc ggacgtcact cggaccgagc tggagccgtg
     2461 ccaggttggc gagggtcggc gtcgggtcac ggtcccccca cggatagcgg cgtttggtcc
     2521 ccttggcgct ccacgacgcc gcgtactccc actccgcttc ggtgggcagg cgcttgtcgg
     2581 cccagcgcgc gaaggcgtcc gcctcgtacc agcacgcgtg ctgcaccggt tcgcccggcg
     2641 cgagatcggc tcggcacccg aactggcgga cactccaggt gctcgcaccc tcgcggcgcc
     2701 agccttcggg ggccaccagt tgcgcctcgt tgcgccacgc ccagcccgcg gtcgaccacc
     2761 agctcggctc gtcatatcca ccgtcgtcga cgaaggctcg gtacgcgccg ttggtgaccg
     2821 gagtccgatc gatgcgaaac ggggggaggt ccaccagatg ggccgggcgc tcgttgtcgt
     2881 acgcccacgg ttcggtgtcg gtccccatga cgaacggtcc gcccgggaga tgcacctcgg
     2941 ggctcccgct gcagtcgggc gcggcgacgc agggacggcg gcctcggctg gtttggtgtg
     3001 gg
//
LOCUS       DATCCH010000487         2638 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_165375, whole genome shotgun sequence.
ACCESSION   DATCCH010000487 DATCCH010000000
VERSION     DATCCH010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165375"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="VIJ44_09390"
     CDS             <1..642
                     /locus_tag="VIJ44_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEY5166152.1"
                     /translation="SGGHSSLLLVPDVTSDVRKLGQTMDDAAGEAFDKVARVLGLPFP
                     GGPYVDRAAREGDPASIAFPRGLMGPRDLADGAEHRFDFSFSGLKTAVARWVELRERD
                     GEPVPVADVAAAFQEAVVDVLTAKAVRAAREHGTADLVLGGGVAANSRLRLVAEERCA
                     AAGVRLRVPRPGLCTDNGAMVAALGAELVRRGRAPSALDHAADSSMPVTQVLA"
     gene            complement(741..1955)
                     /locus_tag="VIJ44_09395"
     CDS             complement(741..1955)
                     /locus_tag="VIJ44_09395"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018562787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY5166153.1"
                     /translation="MAELRTAEGRALLAAAVRGLDAGDDVLPLATALRADGSDPALVA
                     VALTQARLRIRARAKFGVDADRMYWTAAGLEQATRASVAAHRAERYARVAPHLLADLC
                     CGVGGDLVAFARAGLEVLGVDHDPATVAVARANVDELDLAARVDVRQDDVTTTDLTGC
                     DAAFIDPSRRTASGRRRFDPEGYSPPLSYSLALAGRIPATGLKVAPGIPHELVPAAAE
                     VEWVSVGGHVKEAALWFGPLTDAAGRRRATLLPADGTGTTGTTGGSAVTLLDDPTLGA
                     PPVAAAGRYLHEPDGAVIRAGLVAEVADLLDGWLLDPTIAYVSTDSEARSPYTRRYAV
                     EEVVPFQLKRLRALLHARGVGDVVVKKRGTAVEPEELRRRLRLSGDGPTRTLLLTRVS
                     GAPLVLVCDPAP"
     gene            2159..2452
                     /gene="groES"
                     /locus_tag="VIJ44_09400"
     CDS             2159..2452
                     /gene="groES"
                     /locus_tag="VIJ44_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEY5166154.1"
                     /translation="MSVSIKPLEDRILVKSVEAEQTTASGLVIPDTAKEKPQEGEVLA
                     VGPGRFEDGQRLPLDIKVGDKVIYSKYGVTEVKYNGEEYLILSSRDVLAVLES"
BASE COUNT          357 a          990 c          951 g          340 t
ORIGIN      
        1 agcggcggcc actcgtcctt gctgctcgta cccgacgtga cctccgacgt gcgcaagctc
       61 ggccagacca tggacgacgc ggccggcgag gccttcgaca aggtggcgcg cgtgctcggc
      121 ctgcccttcc ccggcgggcc ctacgtcgac cgggcggccc gcgagggcga ccccgcgtcg
      181 atcgcgttcc cgcgcggcct gatggggccg cgggacctgg ccgacggggc cgagcaccgg
      241 ttcgacttct cgttctccgg cctcaagacg gccgtcgcgc ggtgggtcga gctgcgggag
      301 cgcgacggcg aaccggtacc cgtcgccgat gtcgccgccg ccttccagga ggcggtcgtc
      361 gacgtgctca ccgccaaggc cgtgcgcgcc gcacgcgagc acgggaccgc cgacctcgtc
      421 ctcggcggcg gggtcgctgc gaactcccgg ctgcgcctcg tggcggagga gcggtgcgcg
      481 gccgccgggg tccggctgcg ggtgccccgc cccggcctgt gcacggacaa cggggcgatg
      541 gtggcggcgc tcggcgccga gctggtacgc cgcggccggg caccgtcggc cctcgaccat
      601 gccgccgact cctcgatgcc ggtcacccag gtcctggcct gacctggtcc tcgcccggct
      661 tggctgaaac cggggcgggt tgcccggccg gctggccaac cggcgtctgt ctcggccagg
      721 aaggagccgg ggcaggtcag tcatggcgcg gggtcgcaga cgaggacgag aggtgcgccg
      781 gacacccggg tgagcagcag ggtgcgcgtc gggccgtcgc cggacagccg cagccgccgc
      841 cgcagctcct ccggctcgac cgcggtgccg cgcttcttga ccacgacgtc gccgaccccc
      901 ctggcgtgca ggagggcgcg cagccgcttg agctggaacg gtacgacctc ctcgaccgcg
      961 tagcgccgcg tgtacggcga cctggcctcg gagtcggtgc tcacgtacgc gatcgtcggg
     1021 tcgaggagcc acccgtcgag gaggtcggcg acctcggcga ccaggccggc ccggatgacc
     1081 gcgccgtccg gctcgtggag gtaccgcccg gccgcagcga cgggaggcgc cccgagggtg
     1141 gggtcgtcca gcagcgtcac ggctgagccg cccgtggtgc ccgtggtgcc cgtgccgtcg
     1201 gcggggagca gggtcgcgcg ccggcggccg gctgcgtcgg tcagcgggcc gaaccacagc
     1261 gccgcctcct tgacgtggcc gcccacggag acccactcga cctcggcggc cgccggcacg
     1321 agctcgtgcg ggatccctgg tgcgaccttg agcccggtgg cgggtatgcg gccggccagc
     1381 gcgagcgagt acgacaacgg cggcgagtag ccctcgggat cgaagcggcg gcgccccgat
     1441 gcggtccgcc tggacgggtc gatgaacgcg gcatcgcacc cggtgaggtc ggtcgtggtc
     1501 acgtcgtcct gccgtacgtc cacccgggcc gccaggtcga gctcgtcgac gttggcgcgg
     1561 gcgacggcga cggtcgccgg gtcgtggtcg acgcccagca cctcgagacc ggcccgcgcg
     1621 aaggccacga gatcgccgcc gacgccgcag cacaggtcgg ccagcaggtg cggcgccacc
     1681 cgggcgtacc gttcggcgcg gtgcgccgcc accgacgccc gcgtggcctg ctcgagcccg
     1741 gccgcggtcc agtacatccg gtcggcgtcc accccgaact tcgcccgtgc gcggatccgc
     1801 agccgcgcct gggtcagtgc cacggcgacc agcgcggggt cggaaccgtc ggcccgaagc
     1861 gccgtcgcga ggggcagcac gtcgtcgccc gcgtccagcc cgcgtacggc ggcggcgagc
     1921 agggcccgcc cctccgcggt acgcagctcc gccacctggc cgcctctccc tcgcgctcca
     1981 accctacgtg ctgctggcac tcgggttgac ggagtgctaa cggttgccta gtgtgttcga
     2041 cgttggcact ctcaccccga gtgtgccagc gtcccaacca agcgacaccg ggcctggccc
     2101 cgcgacgaca ggcccgcggt cgcctccaaa cagacgaatc gtcggaggta aaccaaccgt
     2161 gtcggtctcc atcaagccgc tcgaggatcg catcctcgtc aagtcggtcg aagccgagca
     2221 gaccaccgcg tcgggcctcg tgatccccga caccgccaag gagaagcccc aggagggcga
     2281 ggttctcgct gtcggtccgg gtcgcttcga ggacggtcag cggctcccgc tcgacatcaa
     2341 ggtcggcgac aaggtgatct acagcaagta cggcgtcacc gaggtgaagt acaacggtga
     2401 ggaatacctc atcctgtcct cgcgtgacgt gctcgccgtc ctcgagagct gatccccggc
     2461 tccaccctgt tcgactgctg cgcgacaacg ccccgggcgg cccagcacaa ccgggctccc
     2521 ggggcgatct cgctccgcac cacccacgaa agggaacccc gccagaatgg ccaagatcct
     2581 cgagttcgac gagaacgccc gccgcagcct tcagcgcggt gtcgacgccc tcgccaac
//
LOCUS       DATCCH010000488         2133 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_165388, whole genome shotgun sequence.
ACCESSION   DATCCH010000488 DATCCH010000000
VERSION     DATCCH010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165388"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="VIJ44_09405"
     CDS             complement(<1..873)
                     /locus_tag="VIJ44_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEY5166155.1"
                     /translation="MTLTDRLLLGPGPSNPYPEATSAFTRPMLGHLDPEFLALLDETM
                     ERLRVVFGTKNRLTFPVSGTGSAGMEACFVNLLEPGDTAIIGVNGVFGERMCEVARRC
                     GAEIVRVDAAWGHAIEPEFLLVAQRTHPRARLVAVVHAETSTGVENEIAPLGALQATD
                     TLLVVDAVTSLAGIPLEVDRWGIDACYSATQKCLGVSPGLAPLTLSERAQARVRSRST
                     PPQSWYLDLGLIERYVEGAQRAYHHTAPIAMIMSLHAALGAILDEGLETVWARHRRVG
                     ARLQHELPRLGFRLL"
     gene            complement(939..1322)
                     /locus_tag="VIJ44_09410"
     CDS             complement(939..1322)
                     /locus_tag="VIJ44_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5166156.1"
                     /translation="MAAPVREPAGEFIGVLRTMTGDDLHRVAASLDSDAICDEVDWWR
                     ATIAIDKVLRHQHCTRRAARAATDAAHAVQDAASRAGIALPDAHITRVARAAAEVARG
                     LAAGASDRPVVGMLLEYWGEITSPV"
     gene            complement(1371..>2133)
                     /locus_tag="VIJ44_09415"
     CDS             complement(1371..>2133)
                     /locus_tag="VIJ44_09415"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5166157.1"
                     /translation="DAIDRTLAVLRDYHHAIDTAGATRVRATATSAARDATNRDEFFT
                     AARDALGFEPELLTGDEEAHLSFTGATAALTDEPAPYLVIDIGGGSTEFTVGTTQPDA
                     AISIDVGCVRITEQFLHTDPPAPEELSAAILTVRDHLGDVERAVPRLHDAKTVVGLAG
                     TITTVAAVEQGVPYSRAHVHRFRLTRDAAEDVFRTLALEPTAGRRHNPGLEPGRVDTI
                     VGGAIVLVATMRALDLDELLVSEDDILDGLARSVA"
BASE COUNT          281 a          629 c          904 g          319 t
ORIGIN      
        1 gaggagccgg aacccgaggc ggggcaattc gtgttggagg cgggcgccga cgcgccggtg
       61 gcgggcccag acggtctcga ggccctcgtc gaggatggcg ccgagcgccg cgtgcaacga
      121 catgatcatg gcgatcggcg cggtgtggtg ataggcgcgc tgcgcgcctt cgacgtagcg
      181 ctcgatgaga ccgaggtcga ggtaccagga ttgcggcggc gtcgaccggg atcggacccg
      241 ggcctgggcc cgttcggaca gtgtgagtgg tgcgaggccg ggcgagacgc cgaggcactt
      301 ctgggtggcg gagtagcagg cgtcgatgcc ccaccggtcg acttcgaggg ggatccccgc
      361 gaggctggtc acggcgtcga cgacgagcag cgtgtcggtg gcttgcagcg cgccgagcgg
      421 ggcgatctcg ttctcgaccc cggtggacgt ctcggcgtgc accaccgcga cgaggcgggc
      481 gcgggggtgg gtgcgctgag cgacgaggag gaactcgggt tcgatcgcgt ggccccaggc
      541 ggcgtcgacg cggacgatct cggcgccgca gcggcgcgcg acctcgcaca tgcgctcccc
      601 gaagaccccg ttgacaccga tgatggcggt gtcgccgggc tcgagcaggt tgacgaagca
      661 ggcctccatc ccggccgacc cggttccgct gaccgggaac gtcagccggt tcttggtgcc
      721 gaacacgacc cggaggcgct ccatggtctc gtcgaggagc gcgaggaact cggggtcgag
      781 gtggccgagc atcggccggg tgaacgcgct cgtggcctcg gggtacgggt tcgagggtcc
      841 agggccgagg aggagccggt cggtgagggt catcgccggt gatcctacga gtgggtgctc
      901 gcggcctcgg ccccgtattc gccgggggct gacgacgctc agaccgggct ggtgatctcg
      961 ccccagtact cgaggagcat gccgaccacg gggcgatccg acgcgccggc ggcgaggccg
     1021 cgtgcgacct cggcggcggc gcgggcgacg cgggtgatgt gcgcgtccgg aagggcgatt
     1081 cccgctcggc tggccgcgtc ttggacggcg tgggcggcgt cggtggcggc gcgcgcggcg
     1141 cggcgggtgc agtgctgatg gcgcagaacc ttgtcgatgg cgatcgtggc ccgccaccag
     1201 tcgacctcgt cgcagatcgc gtcggagtcg agcgacgccg cgacgcggtg caggtcgtcg
     1261 ccggtcatgg tgcggagcac cccgatgaac tcgcccgcgg gttcgcggac cggcgcggcc
     1321 attcgggcat tgtcggacga atggtgcacc aagtggtgga tttcgaggtc ttaggcgacg
     1381 ctgcgggcca agccgtcgag gatgtcgtct tcggagacga ggagctcgtc gaggtcgagg
     1441 gctcgcatgg tggccacgag gacgatggct ccgccgacaa tggtgtcgac ccggccgggt
     1501 tcgaggcccg ggttgtggcg ccgcccggcg gtcggttcga gggcgagggt gcggaagacg
     1561 tcttcggcgg cgtcgcgggt gagccggaag cggtggacgt gtgctcgcga gtaggggacg
     1621 ccttgttcga cggcggcgac ggtggtgatg gtcccggcga gcccgacgac ggtcttggcg
     1681 tcgtgcagcc gggggacggc gcgttcgacg tcgccgaggt ggtcgcggac ggtgaggatc
     1741 gcggcggaga gctcctcggg ggcgggaggg tcggtgtgga ggaactgctc ggtgatgcgc
     1801 acacagccga cgtcgatcga gatggcggcg tcgggttggg tggtgccgac ggtgaactcg
     1861 gtggagccgc cgccgatgtc gatgacgagg tagggggcgg gctcgtcggt gagcgcggcg
     1921 gtggcgccgg tgaaggagag gtgggcttcc tcgtcgccgg tgaggagctc cggttcgaag
     1981 ccgagcgcgt cgcgcgcggc ggtgaagaac tcgtctcggt tggtagcgtc gcgggcggcg
     2041 ctggtggcgg tggcccgcac tcgggtcgcg ccggcggtgt cgatcgcatg gtggtagtcg
     2101 cgcaagacgg ccagcgtccg gtcgatggca tcc
//
LOCUS       DATCCH010000489         2628 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_414093, whole genome shotgun sequence.
ACCESSION   DATCCH010000489 DATCCH010000000
VERSION     DATCCH010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414093"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="VIJ44_09420"
     CDS             complement(<1..280)
                     /locus_tag="VIJ44_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5166158.1"
                     /translation="MIGSAVVPAPWGPIRLAANEDAVVAVDVLATPDSFAAACLRRLG
                     EVGRSSAEVPASVRRRLEQATEAISALLAGEPGAAESVPLALPGASGWD"
     gene            complement(291..1562)
                     /gene="fabF"
                     /locus_tag="VIJ44_09425"
     CDS             complement(291..1562)
                     /gene="fabF"
                     /locus_tag="VIJ44_09425"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEY5166159.1"
                     /translation="MPPSSDPARRAVITGMGCVMPNVGDFPTYWRNLVAGVSGTRRIQ
                     SFDPTNFEVKIAAEVLDFDPTVAMDAKMARRMSRFIQFAMAAGKEAVASSGIDFTAMS
                     EDQRDRVGVVMNTGGGGIEQIIDGTHVADTKGPRFVSPFAVPALSGSMGACLLSMEYH
                     LTGPVITQVAACATSVIAFHDALRLVREGECDVVITGGAEAPVLPMAIAALGNMGALS
                     KRNDDGEHASRPFDSTRDGFVLGEGGGAVIVESLEHALARGATPLAEILGGALTADAF
                     HISAPEPTGRGATRAMTNALRNAGVTTDEIDYIVAHGTATPLNDVTETRAIKAAFGAQ
                     ARRVPISSPKSMIGHLVGAAGIASALAAIGAIRDQVIPPTANLHSPDPECDLDYVPLT
                     ARPAKIDTVMVNGFGFGGQNAVAVFRRYTGA"
     gene            complement(1642..>2628)
                     /locus_tag="VIJ44_09430"
     CDS             complement(1642..>2628)
                     /locus_tag="VIJ44_09430"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEY5166160.1"
                     /translation="SGFEINDENRTEVGVVFGCGAGGQQLMIDNHHILERKGPRFVTP
                     TFIAYGLVDSASGLIAIETGAIGHNVCIASACATGTHNVAEGAEAIRRGDCIAVISGS
                     TESPLLEVAHAGFGNMRGLGMPRPGEPITTVSRPFDKTRDGFVLGEGAGSLFLEDLEL
                     AKARGARIYAEVVGYGSAADGWDMIQPVAHGSGSARAMKMALDRRGVPADEVDLLNPH
                     GTSTPMGDVRESEAIWHVFGDHTPKIAISATKSLTGHMMGAAGSFEAFATVMSVAEQT
                     ITATLNYREPDPECDLFIATETHDARVRYALSNNIGLGGHNGAVIFKHYDGD"
BASE COUNT          377 a          901 c          951 g          399 t
ORIGIN      
        1 gatcccagcc gctcgcgccg ggcagcgcca ggggcaccga ctcggctgcc ccgggttcgc
       61 ccgcgagcag ggcggagatc gcctcggtag cctgctcgag gcgtcgccgg acggaggccg
      121 ggacttcggc ggaggaacgg ccgacctcgc ccaggcgacg caggcaggcc gccgcgaagg
      181 agtccggcgt ggccagcacg tcgacggcga cgaccgcgtc ctcgttggcg gccaggcgga
      241 tcggtcccca gggcgccggg accaccgcgc tcccgatcac gcgagtccga tcatgccccg
      301 gtgtagcgcc ggaagacggc caccgcgttc tggccgccga agccgaagcc gttgaccatg
      361 accgtatcga tcttcgccgg acgggcggtc agcggcacat agtcgaggtc gcactcgggg
      421 tcggggctgt ggaggttggc cgtcggggga atgacctggt cgcggatcgc accgatggcc
      481 gccagggcgc tggcgatccc ggcggcgccc acgagatgcc cgatcatcga cttcggcgag
      541 ctgatcggga cccgcctcgc ctgcgctccg aaggcggcct tgatcgcccg ggtctcggtc
      601 acgtcgttga gcggcgtggc cgtcccgtgg gccacgatgt agtcgatctc gtcggtcgtc
      661 accccggcgt tgcggagggc gttggtcatc gcccgggtcg cgccccggcc ggtcggctcg
      721 ggggcgctga tatggaacgc gtcggcggtc agggcaccgc ccaggatctc ggccaggggc
      781 gtcgccccgc gggccagggc atgctcgagc gactcgacga tcacggcccc gccaccttcg
      841 cccaggacga acccgtcgcg ggtcgaatcg aacggacgcg acgcgtgctc gccgtcgtcg
      901 ttgcgcttgc tcagggcgcc catgttgccc agggcggcga tcgccatcgg caggaccggg
      961 gcctcggcgc caccggtgat cacgacatcg cactccccct cgcggaccag gcggagcgca
     1021 tcgtggaagg cgatcaccga cgtggcgcag gcggcgacct gggtgatcac cggcccggtc
     1081 aggtggtact ccatcgagag caggcaggca cccatcgagc cggacagggc cggcacggcg
     1141 aaggggctga cgaagcgcgg tcccttcgtg tcggcgacgt gggtcccgtc gatgatctgc
     1201 tcgatccccc cgccgccggt gttcatcacg acgccgaccc ggtcgcgctg gtcttcgctc
     1261 atcgccgtga agtcgattcc ggacgaggcc acggcctcct tgccggcggc catcgcaaac
     1321 tggatgaagc ggctcatccg gcgggccatc ttggcgtcca tcgcaacggt cgggtcgaag
     1381 tcgaggacct ccgccgcgat cttcacctcg aagttcgtgg ggtcgaacga ctggatccga
     1441 cgggtgcccg aaacgcccgc caccaggttg cgccagtacg tcgggaagtc gccgacattg
     1501 ggcatcacgc aacccattcc ggtgatcacg gcgcgtcggg cggggtcgga actgggcggc
     1561 acgggctacc tctggggcgg acggtcgggc gcgacgattg tagggcccgg ccgctggcgc
     1621 agcgaccgcg gccgggtcgg gtcagtcccc gtcgtagtgc ttgaagatga ccgccccgtt
     1681 gtgcccgccc agaccgatgt tgttcgaaag tgcgtagcgg acccgcgcat cgtgggtctc
     1741 ggtcgcgatg aagaggtcgc attcggggtc gggttcgcgg tagttgaggg ttgcggtgat
     1801 cgtctgctcg gccaccgaca tgaccgtggc gaatgcctcg aacgagccgg ccgcacccat
     1861 catgtggccg gtcaacgact tcgtggcgct gatcgcgatc tttggcgtgt ggtccccgaa
     1921 cacgtgccag atcgcctcgg attcgcgaac atcgcccatc ggcgtcgacg tgccgtgggg
     1981 gttgagcagg tcgacctcgt cggccggcac tccccgccgg tcgagggcca tcttcatcgc
     2041 ccgggcggag ccgctgccgt gggcgaccgg ctggatcatg tcccagccgt cggccgccga
     2101 gccgtagccc accacttcgg cgtagatgcg cgcgccgcgc gccttggcca gctcgaggtc
     2161 ctcgaggaag agcgagccgg cgccctcccc cagcacaaag ccgtcacggg tcttgtcgaa
     2221 cgggcgcgat acggtcgtga ttggctcgcc gggccggggc atccccaggc cgcgcatgtt
     2281 gccgaaaccg gcgtgggcaa cctcgagcag gggcgactcg gtcgagccgc tgatcacggc
     2341 gatgcaatcg ccgcgccgaa tcgcctcggc accctcagcc acgttgtggg tccccgtggc
     2401 gcaggccgag gcgatgcaca cgttgtggcc gatcgcgccg gtctcgatcg cgatcaggcc
     2461 ggaggccgag tcgaccaggc cgtaggcgat gaaggtcggg gtgacgaagc ggggtccctt
     2521 gcgctcgaga atgtggtggt tgtcgatcat cagctgctgt ccaccggcgc cgcagccgaa
     2581 gacgacgccg acctcggtcc ggttctcgtc gttgatctcg aagcccga
//
LOCUS       DATCCH010000490         2197 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_414669, whole genome shotgun sequence.
ACCESSION   DATCCH010000490 DATCCH010000000
VERSION     DATCCH010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414669"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VIJ44_09435"
     CDS             <1..257
                     /locus_tag="VIJ44_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY5166161.1"
                     /translation="DGVAVNSLAPVAAVRTPGADALVGDVLDANPEIVEPLELFVEAA
                     LALAVADPRLLTGRICYSRPLLDELGLDYRELDGSARVGS"
     gene            complement(261..1427)
                     /locus_tag="VIJ44_09440"
     CDS             complement(261..1427)
                     /locus_tag="VIJ44_09440"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY5166162.1"
                     /translation="MGPRRTLGLVALVAGTAVALSAVPAGAATGGGPVRATYAGPGPY
                     PVGLTTLQLPDRAVSVFYPAKRGSERGKPQASYDQREPYPPDLQALVPAQYDTRITFA
                     AFAGLPGSTTGGPFPVVLFSHGAGSFRLDNASLNAGIASWGFVVVAPEFPERDRAEVT
                     RASLNGGNANSPSSLGLEGQQLRGDADAMLAALRLVETAGRDRHSALDHLVDGKRVAA
                     IGHSAGGGSAFTLLPNPSVKAAIGWAPVPPAQGITEPAKPTMIIAGLDDIAITPARVD
                     SLYGSLPRPKRLVEIGNVGHNGFTDICIAIRQGGGLIEFARQQHLVPDALLALGENGC
                     QMTNLEPRAGFRVIQHFTVAELRFALGIDRSPVGLGNDIVRAFPGVQITYRHSP"
     gene            complement(1489..>2197)
                     /locus_tag="VIJ44_09445"
     CDS             complement(1489..>2197)
                     /locus_tag="VIJ44_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007512156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEY5166163.1"
                     /translation="RAGAPPAVVTASDQPSEYPPIPAATVVLVRDGPDGIESLMLHKN
                     SKLAFGGMWVFPGGRVDPGDRAGAADDGDAARRAAVREAGEEAGLEVDPVSMVWFSHW
                     LPPSIAPKRFATWFFIAPAPSGDVTIDGGEIHDSAWMRPGDALARRDALEIELAPPTW
                     MTLHHLAEFDTVDELLADARQRTPVFYETRVAKGPDGLTAMWRGDAGYESGEPATPGP
                     RHRLRIDAGPWRLERTI"
BASE COUNT          312 a          759 c          804 g          322 t
ORIGIN      
        1 ccgacggggt ggcggtcaac agcctcgccc cggtggctgc ggtgcgcaca ccgggcgccg
       61 acgcgctcgt cggggacgtg ttggacgcca accccgagat cgtcgagccg ctcgagctct
      121 tcgtcgaggc cgcgttggcg ctcgccgtcg ccgacccgcg gttactcaca gggcggatct
      181 gctactcccg tccgctactc gacgagctcg ggctcgacta ccgagagctc gacgggtcgg
      241 cccgcgtcgg atcctgagcc tcagggcgaa tgccggtacg tgatctgcac gccggggaag
      301 gcacggacga tgtcgttgcc gagcccgacc ggtgaccggt cgatcccgag cgcgaatcgc
      361 agttccgcca cggtgaagtg ctggatgacc cggaatccgg cacgcggctc gaggttggtc
      421 atctggcagc cgttctcgcc gagcgccaga agggcgtcag gcacgaggtg ctgctggcgg
      481 gcgaactcga tcaggccgcc cccctggcgg atcgcaatgc agatgtcggt gaatccgttg
      541 tggccgacat tgccgatctc gaccaggcgc ttcgggcgcg gcagtgaccc gtagagcgaa
      601 tcgacccggg cgggggtgat cgcgatgtcg tcgagcccgg cgatgatcat cgtgggcttg
      661 gcgggctcgg tgatcccttg cgccggagga acgggggccc acccgatcgc ggctttcacc
      721 gacgggttgg gcaggagggt gaacgccgag ccacctccgg ccgagtgccc gattgcggcc
      781 acccgcttcc catcgacgag atggtcgagg gctgagtggc ggtcacggcc ggcggtctcg
      841 accaggcgaa gtgccgccag catcgcgtcg gcgtcgcctc ggagctgttg gccctcgaga
      901 ccgaggctcg acggcgagtt ggcgttgccg ccattgaggc tggcccgggt gacctcggcg
      961 cggtcgcgct cggggaactc gggggcgacg acgacgaagc cccaagacgc gattccggcg
     1021 ttgagggagg cgttgtccag ccggaagctt cccgcaccgt ggctgaagag gacgaccggg
     1081 aagggaccac cggtggtgct gccgggcagc cccgcgaacg ccgcgaaggt gatgcgggtg
     1141 tcgtactggg ccggcaccag cgcctgcagg tccggcgggt agggctcgcg ttggtcgtag
     1201 ctcgcctgcg gctttcctcg ctcggagccc cgcttcgccg gatagaagac cgagacggcc
     1261 cggtcaggga gctggagcgt ggtgagcccg accggatacg gaccaggccc cgcgtaggtg
     1321 gcccgcaccg ggccgcctcc ggtcgccgcg cccgcgggca cggccgaaag cgccacggcc
     1381 gtgccggcca ccaaggccac gaggcccagg gtccgtcggg gtcccatggt gcgctgagcg
     1441 tacgcgggtg gccacgacct tcgctgggtc tcgaccggtt cccgtcgctc agatcgtccg
     1501 ctcgagccgc cagggtcccg cgtcgatgcg gagccggtga cgaggtcccg gcgtcgcggg
     1561 ttcaccggat tcgtatcccg cgtcgcctcg ccacatcgcg gtgagaccat cggggccctt
     1621 cgccactcgg gtctcgtaga acaccggcgt ccgctggcgt gcgtcggcga gcagctcgtc
     1681 gacggtgtcg aactcggcca ggtggtgcaa cgtcatccac gtcggcggcg cgagctcgat
     1741 ctcgagcgcg tcccgtcgtg cgagggcgtc acccggacgc atccacgccg agtcgtggat
     1801 ctcgccgccg tcgatggtga cgtcccccga cggtgcggga gcgatgaaga accaggtcgc
     1861 gaaccgcttg ggcgcgatcg acggcggcag ccagtgcgag aaccacacca tcgataccgg
     1921 gtcgacctcg agaccggcct cttcgcccgc ctcgcgcact gcagcccgcc gcgccgcgtc
     1981 accgtcgtcg gcggcaccgg cccggtcgcc ggggtcgact cgaccgcccg ggaacaccca
     2041 catgcctccg aacgccagct tcgagttctt atggagcatc aagctctcga tcccgtccgg
     2101 cccgtctcgg acgagcacga ccgtcgcggc ggggatcggc gggtactccg aaggctggtc
     2161 cgaggcggtc acgacggctg gcggggctcc agcccgg
//
LOCUS       DATCCH010000491         3895 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_39733, whole genome shotgun sequence.
ACCESSION   DATCCH010000491 DATCCH010000000
VERSION     DATCCH010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3895
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39733"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..1845)
                     /locus_tag="VIJ44_09450"
     CDS             complement(184..1845)
                     /locus_tag="VIJ44_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013423104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEY5166164.1"
                     /translation="MEFNLADLFEIVADTVPDRLALVAGEARRTYRDLDERATRLANH
                     LRTAGISPGDHVAVYAWNRAEWLEAELGIYKARAAVINVNYRYVADELEYLLENSDAV
                     AVVFERAFAPLVLAARPHAPPLRHFVVLADGSTDEASEQALAALDAVPYEEALAAASP
                     DRDFGPRSSDDPYLLYTGGTTGLPKGVVWRAEDIFFAALGGGGFGQPPIRSPEELAGR
                     VSTDERGTAALVNAPMMHGGGQWVTFITFYGGNTAVLNCDHHYDGDRVLRLAERERAS
                     SIMVVGDAMARPLADALSAPNASYDLSALAGIGSGGAILSKAVKEELRSLVPEVMVTD
                     SFGASETGAAGTVLDFDGPAAGPKFTVSDWVTVLDDEGQPVEAGSGQIGRLARRGHIP
                     LAYYKDPAKTAATFVTDPDGARWVVPGDYATIEADGTLNLLGRGSVCINTGGEKVYPE
                     EVEATLKAHPDVFDAVVVGVPDERFVERVAAVVTPRPGASVTLEDLQGFCRARLASYK
                     MPRQLHVTDDIPRTPVGKPDYRWAKRLVTQPASASAMATPATPSP"
     gene            1919..2719
                     /locus_tag="VIJ44_09455"
     CDS             1919..2719
                     /locus_tag="VIJ44_09455"
                     /inference="COORDINATES: protein motif:HMM:NF005909.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5166165.1"
                     /translation="MPVETTGLLDGRVAIVSGVGPGLGRSIALTLARAGADVALAARR
                     PEPLTDIAAAVEGLGRRAAVIPTDVADRVQCDRLAAETVDRLGRVDVLVNNAFTEEDW
                     HDPFDGFDPERWREPIAVNLFGSLQLTQAVVPHLRAAGSGSVIMITTLSVKNPVPLLA
                     GYAASKRALTTAAQVMARELGPHHIRVNCVAPGHIRGGSLDEYFAWIAERRGVDVDVV
                     AEEIASMNALPHLATPDEIAGAVLFFASDLSRVVTGQALDVNCGRTLG"
     gene            2743..3522
                     /locus_tag="VIJ44_09460"
     CDS             2743..3522
                     /locus_tag="VIJ44_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY5166166.1"
                     /translation="MLLKDKVGIVSGVGPGLGKEIALAFAREGADLVLGARTESYLAE
                     VQAEVEGLGRRARYAPTDITDQAQCDRLVQTAIDEFGRVDVLVNNAFAADVFQLFEDV
                     DLDAWRRIVDVNLFGTLQLTKSVIPVMKDQGGGSIVFVNSMIVRKILPLQGGYATSKG
                     ALLTAAHVLAKELGPHKIRVNSIVPGWMWGPSVQGYFALMEKQTGKAVQEQYDEIASQ
                     IALGLIPTDDDCANAAVFFASDLSSVITGQALDVNGGEVFH"
     gene            3600..>3895
                     /locus_tag="VIJ44_09465"
     CDS             3600..>3895
                     /locus_tag="VIJ44_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003934407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY5166167.1"
                     /translation="MLRERMVTMGIEPATTVSAGGSCRLLPAADRHWVAMNLARRDDL
                     DLLAAWMRRPWDGPVWDAVAGALHDQPAEAAVERAQLLGIPAAIAVPPAAPRRAS"
BASE COUNT          557 a         1344 c         1388 g          606 t
ORIGIN      
        1 agcccgatcg agcgcaagcc gtgcacgagt tggttggtcg tcgcgagcag cttgcccgcg
       61 ctgacctcgg tgccgtgggg atcgacgagg gccagatggt cagggttcgc ggccgcgatg
      121 gcccagaatc cgagctgggg cgcgtcggtc gtcgtggttc cggtcacgtt ctgctcctgg
      181 tgctcatggg gatggtgtcg cgggcgtcgc catcgcggac gccgacgcgg gctgtgtcac
      241 gagccgcttc gcccagcggt agtcgggctt cccgacgggg gtgcggggga tgtcgtcggt
      301 cacgtgcagc tggcgaggca tcttgtacga ggcgagcctc gcccggcaga acccctggag
      361 gtcttcgagg gtgacgctcg cgccgggtct cggggtgacg accgcggcga cgcgctcgac
      421 gaagcgttcg tcggggacac cgacgacgac ggcgtcaaac acgtccgggt gagccttgag
      481 tgtcgcttcg acctcttcgg ggtacacctt ctcgccgccg gtgttgatgc agaccgaccc
      541 ccgtccgagc aggttcagcg tcccgtcggc ctcgatggtc gcgtagtcgc cggggacgac
      601 ccaccgggct ccgtccgggt cggtgacgaa cgtcgccgcg gtcttcgccg ggtccttgta
      661 gtaggcgagc gggatgtgac ctcggcgggc gaggcggccg atctggcctg agcccgcttc
      721 caccggttgc ccctcgtcgt cgagcaccgt gacccagtcg ctgaccgtga acttggggcc
      781 cgcggccggg ccgtcgaagt ccaggaccgt cccggcggcg ccggtctcgg acgcgccgaa
      841 cgagtccgtg accatcactt ccggcaccag tgatcgcagc tcctccttga cggccttgga
      901 gaggatggcg ccgcctgagc cgattcccgc cagggccgac aggtcatagg aggcgttcgg
      961 cgcgctgaga gcgtcggcga gcgggcgggc catggcgtca ccgaccacca tgatcgagct
     1021 cgcccgttct cgttcggcga gccggagcac ccggtcaccg tcgtagtggt ggtcgcagtt
     1081 gagcaccgcg gtgttgccgc cgtagaaggt gatgaacgtc acccattggc cgcccccgtg
     1141 catcatcggg gcgttcacaa gggccgcggt cccgcgctcg tcggtcgaga ctcgaccggc
     1201 gagctcctcg ggggagcgaa tcgggggctg gccgaagccg ccgccgccga gcgcggcgaa
     1261 gaagatgtcc tcggctcgcc acacgacgcc tttcgggagg ccggtggtgc cgcccgtata
     1321 gagaaggtac gggtcgtcgg acgaccgggg accgaagtcc cggtcgggac tcgcggcggc
     1381 gagcgcctcc tcatacggga cggcgtcgag cgcggcgagg gcctgctccg acgcctcatc
     1441 ggtgcttccg tcggccagga ccacgaagtg gcggagcggg ggcgcgtgcg ggcgcgcggc
     1501 gaggaccagc ggggcgaagg cccgttcgaa gacgaccgcg acggcgtcgg agttctcgag
     1561 gaggtactcg agctcgtcgg cgacgtaccg gtagttcaca ttgatgaccg ccgctcgggc
     1621 cttgtagatg ccgagctcgg cttcgagcca ctcggcccgg ttccacgcgt agaccgcgac
     1681 gtggtcgccg ggactgatcc ccgcggtgcg caggtggttg gcgaggcggg tggcccgctc
     1741 gtcgaggtcg cgataggtcc ggcgggcctc gcccgccacc agcgccaacc ggtccgggac
     1801 ggtgtccgcc acgatctcaa acaggtcggc gaggttgaac tccatcggcc cggaagtaga
     1861 acatgttcct gtccggcccg gcaaggcatg ccgcgtccgg gcacgaggga gccacgccat
     1921 gcccgtcgag accactgggt tgctcgacgg tcgcgtggcg atcgtctctg gggtgggccc
     1981 cggcttgggc cgctcgatcg cgctcaccct cgcccgggcc ggtgccgacg tcgccctcgc
     2041 cgctcgccgt cccgagccgc tgaccgacat cgccgccgcg gtcgaggggc tcggccggcg
     2101 ggcggcggtg atcccgaccg acgtcgccga ccgcgtgcag tgcgaccggc tggccgccga
     2161 gaccgtggac cgtctgggcc gagtcgatgt cctggtgaac aacgcgttca ccgaggagga
     2221 ctggcacgac ccgttcgatg gtttcgaccc cgagcgctgg cgagagccga tcgcggtcaa
     2281 cctgttcggc tcgctgcagc tgacccaagc cgtcgttccg cacctgcgcg ccgccggcag
     2341 cggatccgtg atcatgatca cgaccctctc ggtcaagaac ccggtgccgc tgctcgccgg
     2401 ctacgcggcg tccaagcggg cactcacgac cgcggcgcag gtgatggcaa gagagctcgg
     2461 cccgcatcac atccgggtga actgcgtcgc gcccggccac atccggggcg ggtcgctcga
     2521 cgagtacttc gcctggatcg ccgagcggcg tggagtcgac gtcgacgttg tcgccgagga
     2581 gatcgcctcg atgaacgcgc tgccgcacct cgccacaccc gatgagatcg ccggtgcggt
     2641 cctgttcttc gccagtgacc tgtctcgcgt cgtcaccggc caggccctcg acgtcaactg
     2701 cggtcgaacc ctcgggtaga ccgcgggggg aaaggaaacc caatgctgct caaggacaaa
     2761 gtcggaatcg tctccggggt cggccccgga ctcggcaagg agatcgcact cgcgttcgct
     2821 cgggagggcg ccgacctggt gctgggcgcc cgcaccgagt cgtacctggc cgaggtgcaa
     2881 gccgaggtcg aggggctcgg ccgccgagcc cggtacgcgc ccaccgacat cacggaccag
     2941 gcgcaatgcg accgcctggt gcagaccgcg atcgacgagt tcggccgtgt cgacgtgctc
     3001 gtcaacaacg cgttcgccgc tgacgtcttc cagcttttcg aagacgtcga tctcgacgcg
     3061 tggcgtcgga tcgtcgacgt caacctgttc gggaccctcc agctcaccaa gtcagtgatc
     3121 ccggtgatga aggaccaggg cggcggctcg atcgtcttcg tcaactcgat gatcgtccgc
     3181 aagatccttc cgctccaagg tggctacgcg acgtccaaag gtgcgctcct caccgcggcg
     3241 cacgtgctcg ccaaggagct cggcccgcac aagatccggg tcaactcgat cgttcccggt
     3301 tggatgtggg gcccgtcagt gcagggatac ttcgcactca tggagaagca gacgggcaag
     3361 gcggtccagg agcagtacga cgagatcgcc tcgcagatcg cgctggggct catcccgacc
     3421 gatgacgact gcgcgaacgc tgccgtgttc ttcgcgtccg atctctcgtc ggtcatcacc
     3481 ggccaagctc tcgacgtcaa cggcggcgag gtgttccact gacccgccgc gacgccgaag
     3541 cggccctcga tgcggccgcg acccgagccg gtgcccggct gggtgtcgac gggcccgcct
     3601 tgctccgcga acgcatggtg accatgggca tcgagccggc gacgaccgtc tccgccggcg
     3661 ggtcgtgccg cctcctcccc gccgccgatc gtcactgggt ggcgatgaac ctcgcccggc
     3721 gcgacgacct cgacctgctc gcggcgtgga tgcgacggcc ctgggacggc ccggtttggg
     3781 atgcggtcgc cggcgccctg cacgaccagc ccgcggaggc cgcggtcgaa cgggcccagc
     3841 tgctcggcat ccccgccgcg atcgcggtgc caccggctgc gcctcgccgg gcctc
//
LOCUS       DATCCH010000492         2241 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate SMAG_U7694
            k141_40737, whole genome shotgun sequence.
ACCESSION   DATCCH010000492 DATCCH010000000
VERSION     DATCCH010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37962069
            Sequence Read Archive: SRR3985394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 14:10:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,896
            CDSs (total)                      :: 1,876
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 20
            tRNAs                             :: 18
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 12
            CDSs (without protein)            :: 12
            Pseudo Genes (ambiguous residues) :: 0 of 12
            Pseudo Genes (frameshifted)       :: 2 of 12
            Pseudo Genes (incomplete)         :: 11 of 12
            Pseudo Genes (internal stop)      :: 1 of 12
            Pseudo Genes (multiple problems)  :: 2 of 12
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40737"
                     /isolate="SMAG_U7694"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.2999 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VIJ44_09470"
     CDS             <1..255
                     /locus_tag="VIJ44_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009612384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEY5166168.1"
                     /translation="EPIWAIGTGRNATPDDADTTIGVIRDALRSLFAGTADEIRIQYG
                     GSVKPGNIADLMRRPEIDGALVGGASLDPDDFARIVQYGR"
     gene            293..526
                     /gene="secG"
                     /locus_tag="VIJ44_09475"
     CDS             293..526
                     /gene="secG"
                     /locus_tag="VIJ44_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEY5166169.1"
                     /translation="MLNAIILVVDIAAALTLIFLILLHSGRGGGLSDMFGGGQLGGSM
                     AGSTVVERNLDRLTIVAATVFTFATVILSLRLQ"
     gene            676..>2241
                     /locus_tag="VIJ44_09480"
     CDS             676..>2241
                     /locus_tag="VIJ44_09480"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY5166170.1"
                     /translation="MNSPRWLRGAVAVALGGLLASVVATPTLAVAGRARPRAASGGEI
                     TIGAEQEPSCADWIDACAGETWGVWTMEEHTMPRVFDIVARGGQWVYQPSVLMAAPPT
                     VSTVGGNQVVTYQLNPKAVWSDGQPITATDFKYTWDQIAHGANIYDRTGYDRIASVDD
                     SNPRTAVVTFSSSFAGWRQLFGGGFYGVFPSHLLAGKDRDAQMKDGYTWSGGPFKIQT
                     WDKGVRIVLVPNPNYWGPKPKLAKVTFRFLTDTAAEFQAFQGGEVSAIYPSPEPDVVR
                     TLKRGISGSSTVFNANTGNIEALWMNNAAFPFDSVKVRQAVGYAIDRDAIVRLLFAPL
                     GVTHATNSLNPPILAPYSDQAAFAGYHRDLRKVDQLMTADGWQKSGGIWQKNGRAAQF
                     TIQSTAGDKRRELIEQVLQQQLHEAGFTLTVDNKDANSLFGQILPAGNFQLGLYADVV
                     TDLDPGLCATFCAKNIPTAANSTSGQNYTRTSIPTLDPLLETVDSTTTDAARVQASKA
                     ADRILADQQVTLPL"
BASE COUNT          366 a          867 c          675 g          333 t
ORIGIN      
        1 gagccgatct gggccatcgg caccggtcgc aacgccaccc ccgacgacgc cgacaccacc
       61 atcggcgtca tccgcgacgc cctccgctcg ctgttcgccg ggaccgccga cgagattcgc
      121 atccagtacg gcggcagcgt caagcccggc aacatcgccg atctcatgcg ccgccccgag
      181 atcgacgggg ccctcgtcgg tggcgcgtcg ctcgaccccg acgacttcgc ccgcatcgtg
      241 cagtacgggc gctgacgccg agcccgtcac ggccggcagg taggatgatt aggtgctcaa
      301 cgcgatcatc ctcgtcgtcg acatcgcggc cgcgctgacc ctgatcttcc tcatcctctt
      361 gcactccggg cgaggcggcg ggctctccga catgttcggc ggcggccagc tcggcgggtc
      421 gatggctggc tccaccgtcg tggaacgcaa cctcgaccgc ctcaccatcg tcgccgccac
      481 cgtcttcacc ttcgccaccg tcatcctttc cctgcgtctc cagtagcacc accccctgac
      541 cgcgacggcc cgaccgcggc gcgctcggcg cgacgcgtcc caggcgtcac cggcgcccct
      601 cgccggtatc ccccgaatcg agcggggcct gtgatagcac ggtccctcgg ccggaccgga
      661 caagggggtc gtggcgtgaa ctcgcctcgt tggctccgcg gcgcggtcgc ggtggcgctc
      721 ggcgggctct tggcctctgt cgtcgcgacg ccgacgctcg cggtggccgg ccgggcccgt
      781 ccccgggccg cgtcgggtgg cgagatcacg atcggtgccg agcaggagcc cagctgcgcc
      841 gactggattg acgcctgcgc cggggagacc tggggtgtgt ggacgatgga agagcacacc
      901 atgccaaggg tgttcgacat cgtggcccgc ggcgggcaat gggtgtacca gcccagtgtc
      961 ctcatggccg cgccccccac ggtctccacc gtcggtggca accaggtcgt cacctaccag
     1021 ctcaacccga aggcggtctg gtccgacggg cagccgatca ccgccaccga cttcaagtac
     1081 acgtgggacc agatcgccca cggcgccaac atctacgacc gcaccggcta cgaccgcatc
     1141 gcgtccgtcg acgactccaa tcctcggacc gcggtggtca ccttcagctc ctcgttcgcc
     1201 ggttggcgcc agctgttcgg cggcggcttc tacggcgtgt tccccagcca tctgctcgcc
     1261 ggcaaggacc gcgacgccca gatgaaggac ggctacacct ggtccggcgg accgttcaag
     1321 atccagacct gggacaaagg ggtccgcatc gttctcgtcc ccaaccccaa ctactggggg
     1381 cccaagccga agctggccaa agtcacgttc cggttcctca ccgacaccgc ggcggagttc
     1441 caggccttcc aaggtggcga ggtgtcggcg atctacccgt cccccgagcc ggacgtcgtc
     1501 cgcaccctca aacgggggat cagcggcagc agcaccgtgt tcaacgcgaa caccggcaac
     1561 atcgaagcgt tgtggatgaa caacgccgcg ttcccgttcg actcggtcaa ggtccgccag
     1621 gcggtcgggt acgccatcga ccgtgacgcc atcgtccggc tcctcttcgc tccgctcggg
     1681 gtgacgcacg ccaccaactc gctcaacccg ccgatcctcg ccccgtacag cgaccaggcc
     1741 gcgttcgccg ggtaccaccg tgatctccgc aaggtcgacc agctcatgac cgccgacggc
     1801 tggcagaagt cgggggggat ctggcagaag aacggccgcg cggcccagtt cacgatccaa
     1861 tccaccgccg gtgacaagcg gcgcgagctc atcgagcagg tcctccaaca gcagctccac
     1921 gaggccggct tcaccttgac tgtcgacaac aaggacgcca acagcctctt cggccagatc
     1981 ctccccgccg ggaacttcca gctcgggctc tacgccgacg tcgtcactga cctcgacccg
     2041 gggctgtgcg ccaccttctg tgcgaagaac atcccgaccg ccgccaacag cacctcgggc
     2101 cagaactaca cccgcaccag catccccacc ctcgacccct tgctcgagac ggtcgacagc
     2161 accaccaccg acgccgcccg ggtccaagcc tcgaaggccg ccgatcggat cctcgccgac
     2221 cagcaggtca cgctccccct c
//
